LOCUS       DAXHSF010000001        55645 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.001, whole genome shotgun sequence.
ACCESSION   DAXHSF010000001 DAXHSF010000000
VERSION     DAXHSF010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 55645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55645
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.001"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            52..1005
                     /locus_tag="ACFM8S_000001"
     CDS             52..1005
                     /locus_tag="ACFM8S_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFC6313155.1"
                     /translation="MTHTVHLHLEETDNLALQRLCGSFDNNLDLLAKALDIHISRRFE
                     HFTFNGAFAHAGKRALLKLLETAQTRDLNDGDIRLAAVEAQTEDAGHQEKNHDHAYYF
                     RTKRGSIGGRTPRQNGYIRALLNHDIVFGLGPAGTGKTYLAVAAAVDAMEKHQVERII
                     LVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVTKLIEKGLIEIAPLA
                     YMRGRTLNGAYIILDEAQNTTPEQMKMFLTRIGFGAKAVITGDTSQIDLPKNIKSGLK
                     DAREKLHNVEGLYFHTFTGEDVVRHPLVQKIVEAYESAEHD"
     gene            complement(1109..1432)
                     /locus_tag="ACFM8S_000002"
     CDS             complement(1109..1432)
                     /locus_tag="ACFM8S_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313156.1"
                     /translation="MKKCILGILTACAAMPAFADRIGDLEARLAQLEHRVAVLESGSN
                     TVKIDLFGSNSTMYVCSVTPFQKTFEASDRNEGVARQKVRQACNRETSAMFCEDEAIR
                     CRKFD"
     gene            complement(1580..2941)
                     /locus_tag="ACFM8S_000003"
     CDS             complement(1580..2941)
                     /locus_tag="ACFM8S_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFC6313157.1"
                     /translation="MLKKWLNKMLPSGRSSKKAESKTVIPAERHNIRAEMLSFAAENV
                     IRRLKGAGFQAYVVGGAVRDLLLGIEPKDFDVATDATPEQVHKLFRRSRIIGRRFQIV
                     HVMNGAEIIEVTTFRGGAKVHQNAHGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
                     EILDFHNGIADVAARRLVMIGDAAERYQEDPVRILRAIRLSGKLGFDLSEETAAPIAE
                     SICRLKHEPVARLFDEIMKLLFSGHARECLKRLNGFDIPDDIHPLLNALRVSDGIAGK
                     MTVLALKNTDERLRADKSVSVGFVLAALMWPELERHWKSNLQQGLKPVPALSDAINTM
                     RETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAET
                     GNADRALAEWWTAFQTASQEQRTEMTKNEAAARHEKNEGQAKKRRRRRRKPKPKVEGT
                     DWE"
     gene            complement(3302..5002)
                     /gene="recJ"
                     /locus_tag="ACFM8S_000004"
     CDS             complement(3302..5002)
                     /gene="recJ"
                     /locus_tag="ACFM8S_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFC6313158.1"
                     /translation="MSVKIQTRSINTDVFNHLLTAGADPLIARLCASRGVQSPAELDD
                     KLAALLPYQTLTNCEAAAGRLADAVGRKEKILIVADYDADGATACAVGLDGLAAMGAK
                     VDFLVPNRFEHGYGLTPELAEIAAAQGVDLLITVDNGIASIAGVARAQALGLDVIVTD
                     HHLPADTVPDCIIVNPNQKGCGFQSKSLAGVGVIFYVLTALRAELRRRNYFSDGLKEP
                     NLGDLLDLVALGTVADVVPLDHNNRILVSQGLKRMRSGKMRPGIRALFEVARRDWRKA
                     QPFDMGFALGPRINAAGRLDDMSVGIACLLARDDSEAQELAAQLNNLNIERREIEQSM
                     LQDALNAFPETLPSGQTTLVAYRDDFHQGVVGIVASRLKDRFYRPTIVFAPADNGEVR
                     GSGRSIPNLHLRDALDLVSKRHPDLILKFGGHAMAAGLSILEHNIPAFQTVFEEAVRE
                     MVCEDDLSQTFITDGSLKACDITLEQAQNLARHVWGQGFAPPSFTDEFHVVRQQPLGA
                     EGKHKKAWLQKDGCEFEAMFWRCSEDIPEYIRTVYRPVANEWRNNLELQLYIDYWEAV
                     "
     gene            complement(5071..5789)
                     /locus_tag="ACFM8S_000005"
                     /pseudo
     CDS             complement(5071..5789)
                     /locus_tag="ACFM8S_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258153.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5836..6348)
                     /gene="yjgA"
                     /locus_tag="ACFM8S_000006"
     CDS             complement(5836..6348)
                     /gene="yjgA"
                     /locus_tag="ACFM8S_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFC6313159.1"
                     /translation="MFEQEDEWVSKTQMKKQMNGLQDLGMELTKLSSDTLKKIGLDED
                     LYEAIVTYKKITSNGALKRQAQFIGRLMRDTDPAPIEAFLAKLRGDDAAHNAFLQRVE
                     RTRMELLADDGALTQFMSDFPNADAGKLRTLIRNTKKEQEQNKPPKNFRALFQELKTV
                     MESQGGTGEA"
     gene            6502..7833
                     /gene="pmbA"
                     /locus_tag="ACFM8S_000007"
     CDS             6502..7833
                     /gene="pmbA"
                     /locus_tag="ACFM8S_000007"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFC6313160.1"
                     /translation="MLFNHTASELLDLCRRTLDLAKAAGATAAEADFSESIGQSVSVR
                     LGEIEQIEFQQDKSLDITVYVGKRKGRAGTADFSEQALRDTVRAAADIARHTAEDGCA
                     GLADACLMAKHIGDPDLYHEWDLDTEAAVELAKQCEQAALNEDERIENSEGAAVQTGH
                     YQYVYGNTHGFAAHRQGTHHSISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQT
                     AARRTLRRLGSRSIPTGSYPVLFDTTVSGGLIGHLVGALSGGALYRQSSFLIDSIGKK
                     VLPDFLNLREEPHIPRSFRSSYFDAEGVATAPRFVIQNGIVKGYFLSSYSARKLGMQT
                     TGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVEN
                     GVIAYPVHEITVAGRLQDMYRDIVGVADDALRRSSNKIGSILIAGMTVAGS"
     gene            complement(8130..8333)
                     /locus_tag="ACFM8S_000008"
     CDS             complement(8130..8333)
                     /locus_tag="ACFM8S_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFC6313161.1"
                     /translation="MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMEGFKTLKE
                     GQRVSFDVTTGPKGKQAANIQAA"
     gene            8576..8878
                     /gene="clpS"
                     /locus_tag="ACFM8S_000009"
     CDS             8576..8878
                     /gene="clpS"
                     /locus_tag="ACFM8S_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFC6313162.1"
                     /translation="MTAQHQSDTLLHRLNTLPPKRYGVFLLNDDYTTMEFVVEILTEI
                     FMLGQEQAVAVMLLVHHEGKGLCGTYTRDIAQTKQQQVMQRAKAEGHPLQCIVEEI"
     gene            8880..11171
                     /gene="clpA"
                     /locus_tag="ACFM8S_000010"
     CDS             8880..11171
                     /gene="clpA"
                     /locus_tag="ACFM8S_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFC6313163.1"
                     /translation="MLAPELEQILQQLYREARKAHYEFISLEHLLLVLIEEDASVPNV
                     LKLCGADLKVVSEQLAASVAENTPLIPEHLLDTVETRPTLGFQRVMQRAMVHTQSAGK
                     AAVEPLDVLVALMSETDSHTVYFLKLQSVTRFEVLRCIAHGSPDEDEDDGNYSSDGMD
                     DDNENRTKPGKNPLSAYTVNLNAEVKAGRIDPLIGRKHEMERLVQILCRRRKNNPLLV
                     GEAGVGKTALAEGLAHQIVNGGIPDALKDAEVYALDMGSLLAGTKYRGDFEARVKSVL
                     KQLEKIPHAILFIDEIHTIIGAGSTSGGTMDASNLLKPALAKGSLRCIGATTYDEYRT
                     IFDKDHALSRRFQKIDVVEPTVSETVQILRGLKPMFEAFHQVRYTQGALEAAAELSAR
                     YINERFLPDKAIDVMDEAGAAQRILPKSKQKKVIGKAQIETVIAKVARIPEKTVSHDD
                     KQVLQFLGRDLKNMVYGQENAIDALVAAVKMSRSGLALPDKPIGSFLFSGPTGVGKTE
                     AAKQLAYSLGVPLQRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEAINKQPHCVL
                     LLDEIEKAHPDIFNVLLQVMDAGKLTDNNGKSADFRNVILIMTTNAGAESLSRPSLGF
                     TAKRERGDEMQAINKLFTPEFRNRLDAIIPFAPLSEPVITKVVDKFLLQLEHRLLDKK
                     VEAEFTSALHKYLAEKGFDPQMGARPMHRLIQEKIRKPLADELLFGKLSDGGFVRIDW
                     DAAKEEAVLKFKKSKVKIKTASA"
     gene            complement(11210..14308)
                     /locus_tag="ACFM8S_000011"
     CDS             complement(11210..14308)
                     /locus_tag="ACFM8S_000011"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFC6313164.1"
                     /translation="MNLETKPIAETPNFIVLDQYEKIEQSGSYQSENRLEAELIADLQ
                     NQGYEYRKDLNSQSRLLENLRAQLQRLNDVAFSDGEWARFLTEYLDRPSENITDKTRK
                     IHDDHIYDFAFDDGRLKNIYLLDKKNLARNHVQLINQFEQTGTHANRYDVTVLVNGLP
                     LVQIELKKRGVAVREAFNQVHRYSKESFNSENSLFKFLQIFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWARSDNHPIKDLKDFTATFLQKSVLLSVLLHYSVFDANNTLLIMRPYQ
                     IAAAERILWKINSSALAKNWSKPESGGYVWHTTGSGKTLTSFKAARLATESAFIDKVF
                     FVVDRKDLDYQTMKEYQRFSPDSVNGSESTTGLKRNLEKDDNKIIVTTIQKLNNLMKS
                     EDNLPVYHQRVVFIFDECHRSQFGEAQKNLKKKFKKFCQFGFTGTPIFPENALGAETT
                     AGVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKAVEAEQDEKKLSAAENRQALL
                     HPERIREITQYILNQFRQKTHRLNAGGKGFNAMFAVSSVDAAKCYYEAFKTQQAGSLH
                     PLKVATIFSFAANEEQNAVGEIVDETFEPEAMDSSAKEFLQAAINDYNACFKTNFGTD
                     SKAFQNYYRDLAKRVKNQEVDLLIVVGMFLTGFDAPTLNTLFVDKNLRYHGLMQAFSR
                     TNRIYDATKTFGNIVCFRDLEQATIDAITLFGDKNTKNVVLEKSYEEYMNGYTDSQTG
                     EARRGYLDVAKELRQRFPDPDKIETEKDKKDFAKLFGEYLRAENVLQNYDEFAALREL
                     QSVDAADEDAMKAFQEKYYLSDEDVQEMRQVPMPSERAVQDYRSAYNDIRDWLRRQKA
                     GEQKEQSKIDWDDVVFEVDLLKSQEINLDYILQLVFEHHKKIKGKAELVEEIRRIIRA
                     SIGHRAKEGLIVDFINDTDLDKVPDVPAILETFYTYGQEVMRHEAAGLIAAEGLNETA
                     AKRYLTGSLKRGYASENGTELTETLPKMSPLNPQYLTKKQSVFQKIAAFVEKFAGIGT
                     DI"
     gene            complement(14738..15325)
                     /locus_tag="ACFM8S_000012"
     CDS             complement(14738..15325)
                     /locus_tag="ACFM8S_000012"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFC6313165.1"
                     /translation="MVWKTLGEVFDLKNGYTPSKSNKEYWENGSIPWFRMEDIRENGR
                     ILDNSLQHISKSAVKGGKLFPANSIIMATSATIGEHALITVPFLANQRFTSLSLKPEF
                     ADKLSIYFLYYYCFNLSEWCKKNTTTSSFASVDMNGFKRFPIPIPPLPEQEKIVAILD
                     KFDTLTHSISEGLPHEIALRRKQYEYYREQLLAFR"
     gene            complement(15351..15947)
                     /locus_tag="ACFM8S_000013"
     CDS             complement(15351..15947)
                     /locus_tag="ACFM8S_000013"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFC6313166.1"
                     /translation="MDMQSKAKKLIEMIQTAPVEWKPLGGENGIAIIKTGQAVSKQKI
                     SNNIGSYPVINSGKEPLGYIDEWNTENDPIGITTRGAGVGSITWQEGRYFRGNLNYAV
                     TIKNRTELDVRFLYHILLEFEQEIHALCTFTGIPALNASNLKKLLIPIPPLETQQKIV
                     KILDKFTELEAELALRKRQYRYYRDFLLDFDNQIGGDS"
     gene            complement(16113..17123)
                     /locus_tag="ACFM8S_000014"
     CDS             complement(16113..17123)
                     /locus_tag="ACFM8S_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HFC6313167.1"
                     /translation="MSIILYTANDGTAQFALQEFGGQLWLTQADMAELYQTTKQNISK
                     HIKTILAEQELEEKATVNFQLTVQNENGRKVNRKIAHYSLPMIIAVGYRVRSARGIQF
                     RQWATERLDEYLTKGFAIDDERLKGTGGGDYWKELLNRIRDIRSSEKALYRQVLDLYA
                     TSQDYNPKSSESQTFFAAVQNKLHYAASRQTAAELIYSRADSSKDFMGLTTFQGAIPT
                     LNEAKIAKNYLTEDELFRLNRLVSAFFDLAEIKAQEQSPMYMRDWIAELDKFSGLYGQ
                     GTLQGAGSISRKQAEQKAEREYRAYEARILSPVEQAYLESVKALEKTAVQQIKQKKDR
                     TK"
     gene            complement(17120..18670)
                     /locus_tag="ACFM8S_000015"
     CDS             complement(17120..18670)
                     /locus_tag="ACFM8S_000015"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFC6313168.1"
                     /translation="MMTEMQQRAQLHRQIWKIADEVRGAVDGWDFKQYVLGTLFYRFI
                     SENFTDYMQAGDSSIDYAAMPDSIITPEIKDDAVKVKGYFIYPGQLFCNIAAEAHQNE
                     ELNTKLKEIFTAIESSASGYPSEQDIKGLFDDFDTTSSRLGSTVADKNKRLAAVLKGV
                     AELDFGSFEDHHIDLFGDAYEYLISNYAANAGKSGGEFFTPQSVSVSKLIARLAVHGQ
                     EKVNKIYDPACGSGSLLLQAKKQFDEHIIEEGFFGQEINHTTYNLARMNMFLHNVNYN
                     KFHIELGDTLTNPKLKDSKPFDAVVSNPPYSINWIGSGDPTLINDDRFAPAGVLAPKS
                     KADFAFILHALNYLSGRGRAAIVSFPGIFYRGGAEQKIRQYLVEGNYVETVIALAPNL
                     FYGTGIAVNILVLSKHKDNTDIQFIDAGGFFKKETNNNVLTEEHIAEIVKLFADKADV
                     PHIAQNAAQQTVKDNGYNLAVSSYVEPEDTREIIDIKQLNAEISETVAKIERLRREID
                     EVIAEIEA"
     gene            complement(18743..19747)
                     /gene="rfaD"
                     /locus_tag="ACFM8S_000016"
     CDS             complement(18743..19747)
                     /gene="rfaD"
                     /locus_tag="ACFM8S_000016"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFC6313169.1"
                     /translation="MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLSKGEKFKNLA
                     ECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGACSDTMNHDGLYMMENNYQYTLDL
                     LDWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKEGL
                     TAQVVGFRYFNVYGQHEQHKGRMASVAFHHFHQYREHGYVNLFGSNDGYGNGEQTRDF
                     VSVEDVAKVNLYFFDHPELSGIYNLGTGRSQQFNELAAATVNACRAAEGKPEMSLKEL
                     VEEEFIRYIPFPDALKGKYQSFTQADITKLREAGYKEEFFDVKSGVDRYVKWMLENLA
                     "
     gene            complement(19788..20907)
                     /locus_tag="ACFM8S_000017"
                     /pseudo
     CDS             complement(19788..20907)
                     /locus_tag="ACFM8S_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221166.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            complement(20909..22144)
                     /locus_tag="ACFM8S_000018"
     CDS             complement(20909..22144)
                     /locus_tag="ACFM8S_000018"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC6313170.1"
                     /translation="MTISTMAQTHDTRLQKTLLFPSQQGWKSKTFLKPDSHIRLATVF
                     SGIGAVEQAFHRLNLNHTIVFAGDIDPYVKKSYLGNYKLNEDFWHNDITQFDARKFRN
                     QVDILVGGSPCQAFSMVGKRAGLEDTRGTLFYEFARVVDEVQPKIFIYENVKGLLNHD
                     NGKTWKVVKSVFYSLGYDLYFQIMNSKDYGIPQHRERIFVVGFHTPPINGFQFPEKIE
                     LEHTMQDFLEDYTDSKYFLREKGVKFVTSSKNRQKRYTQINGEIALCQKANQQFNWHG
                     DFIFQAARESEFDDFIFDVNNVEEKYYLSEKIKNYVLAGGTKNFKTSTETDLPVARPL
                     LQTMHKMHRAGVDNYVTHNRGRIRKLTPRECLRLMGFRDDFKILVSDTQMYRQAGNSI
                     VVDVLIAILKQMDITLYGE"
     gene            complement(22181..23152)
                     /gene="hldA"
                     /locus_tag="ACFM8S_000019"
     CDS             complement(22181..23152)
                     /gene="hldA"
                     /locus_tag="ACFM8S_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose synthase"
                     /protein_id="HFC6313171.1"
                     /translation="MSAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPV
                     PVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTGNDEAADALDALMVQDGVASYLM
                     RDKQIATTVKLRVVARNQQLIRLDFEEHPNCEVLEQIKQKYREILPEYDAIIFSDYGK
                     GGLSHISDMIDWAKHAGKTVLIDPKGDDYEKYVGATLITPNRAELKEVVGSWKNESEL
                     TEKAQNLRRHLDLTAVLLTRSEEGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMG
                     LGLAAGCTMPEAMYLANTAAGVVVAKLGTAVCSFAELIKALSGQSTM"
     gene            complement(23207..23947)
                     /gene="pyrF"
                     /locus_tag="ACFM8S_000020"
     CDS             complement(23207..23947)
                     /gene="pyrF"
                     /locus_tag="ACFM8S_000020"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFC6313172.1"
                     /translation="MNPLISDFQTPQQRTPVIVALDFSNEKDTLGFVRNLDPTLCQIK
                     IGKELFTATGRNLAESLINQGFKLFLDLKYHDIPHTVAQACKVAADMGVWMVDMHASG
                     GRRMMEAAAEAVAGYGTKPLLIGVTVLTSMEQSDLAEIGLNTAPEEQVIRLAKLAQSS
                     GLDGVVCSAQEAAPLRRELGQDFVLVTPGIRLDVAGNNDDQRRIMTPAEALAAASTYL
                     VMGRPVTQAADPVAVLREVNRVANLEAN"
     gene            complement(24473..25120)
                     /gene="adk"
                     /locus_tag="ACFM8S_000021"
     CDS             complement(24473..25120)
                     /gene="adk"
                     /locus_tag="ACFM8S_000021"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFC6313173.1"
                     /translation="MKALLLGAPGAGKGTQAQFITAAFGIPQISTGDMLRAAIKAGTP
                     LGLEAKKIIDEGGLVRDDIIIGMVKERIAQDDCKNGFLFDGFPRTLAQAEAMVEAGVD
                     LDAVVEIDVPDSVIVDRMSGRRVHLASGRTYHVTYNPPKVEGKDDVTGEDLIQRDDDK
                     EETVKKRLAVYHEQTEVLVDFYSKLEGEHAPKYIKVDGTQPVEAVKAEVLGALGK"
     gene            25335..26174
                     /gene="htpX"
                     /locus_tag="ACFM8S_000022"
     CDS             25335..26174
                     /gene="htpX"
                     /locus_tag="ACFM8S_000022"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFC6313174.1"
                     /translation="MKRIFLFLATNIAVLVVINIVLAVLGINSRGGAGSLLAYSAVVG
                     FSGSIISLLMSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHS
                     PEPNAFATGASRNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGVV
                     NTFVVFLSRIIANLIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRA
                     DAGAAKLVGAPKMISALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARL
                     KSL"
     gene            complement(26475..27035)
                     /locus_tag="ACFM8S_000023"
     CDS             complement(26475..27035)
                     /locus_tag="ACFM8S_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313175.1"
                     /translation="MTANFAQTLVEIQDSLYRVVSTVQYGDDNLKRLTADKRKQYELN
                     FKISEGSTRVESDFKETLVRFGRDMLQDMPPKIRSATLVALTTLLVGGALGYGYLEYL
                     KQVASEGYQTERLYNAVDRLAESQERITSAILKGARGADFVQIGRRSYSREDISEANR
                     RAERVPYGAELVSDGNFTAVLSDIGD"
     gene            complement(27149..27346)
                     /locus_tag="ACFM8S_000024"
     CDS             complement(27149..27346)
                     /locus_tag="ACFM8S_000024"
                     /inference="COORDINATES: protein motif:HMM:NF019096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD1 domain-containing DNA-binding protein"
                     /protein_id="HFC6313176.1"
                     /translation="MKENTPPQNQKPVSILDTATGETLTFPSVQAAAKALNTPYGSIR
                     TKLDGRSNPDNLVCNRYKVLL"
     gene            complement(27368..27541)
                     /locus_tag="ACFM8S_000025"
                     /pseudo
     CDS             complement(27368..27541)
                     /locus_tag="ACFM8S_000025"
                     /inference="COORDINATES: protein motif:HMM:NF017109.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
     gene            complement(27749..28141)
                     /locus_tag="ACFM8S_000026"
     CDS             complement(27749..28141)
                     /locus_tag="ACFM8S_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HFC6313177.1"
                     /translation="MAKSFNQAASELTDIFPNISLTDFDGVNYPVTVNCPMHGNVRYS
                     TFNALIKSKYGCPECAKMSKTQTPPNVGKPLLILDTTTNETLTFPSVTAAGAALGVHF
                     QQINHRLKGRTSPDNLISNRYKVLGYDR"
     gene            complement(28258..28668)
                     /locus_tag="ACFM8S_000027"
     CDS             complement(28258..28668)
                     /locus_tag="ACFM8S_000027"
                     /inference="COORDINATES: protein motif:HMM:NF017109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HFC6313178.1"
                     /translation="MALTFTQAVSKLTSKFPHLNLVEFNGVRYPTVIVCPIHGRVTCS
                     TFKSMLDSKSGCPKCASYGVNSHKIPEDTIDKLSKNTVLEDTVTGETLTFPSRASAAR
                     SLGINPAAITDRIKGRVHTETLLAGRYKVHICTK"
     gene            complement(28682..29065)
                     /locus_tag="ACFM8S_000028"
     CDS             complement(28682..29065)
                     /locus_tag="ACFM8S_000028"
                     /inference="COORDINATES: protein motif:HMM:NF017109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HFC6313179.1"
                     /translation="MPKSLSLQDVQLRFSSKFPDKTVLKFTKNYEPVTIQCPLHGSVV
                     YGNLQAAMKSSTGCPECTRNPEKPSPNAVAIRLEDTETGEIYEFASTLAASKFIGCSN
                     STLAVRLSGRTPFDRLIRYRYRLVK"
     gene            complement(29531..30829)
                     /locus_tag="ACFM8S_000029"
     CDS             complement(29531..30829)
                     /locus_tag="ACFM8S_000029"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFC6313180.1"
                     /translation="MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTL
                     VVGGKKTILRLIPSGILHESLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIA
                     PTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRAADLLHPEKLR
                     EKLDAVLAYYNVQLQHLHNAEPVKAEDVMAVIEKVAPRIAPMITDVSRVLNEKNKNGE
                     KLLFEGAQGALLDIDYGTYPFVTSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVG
                     SGPFPTELFDEVGAGLAERGHEFGSVTGRARRCGWFDAAALKRSIQINGISGMCITKL
                     DVMDGVETINICVGYELPDGGKTDILPCGSDAVEACKPIYETMPGWSESTFGVKSYDA
                     LPANAKAYLKRIEEVCGAPVAIVSTGPDREETIVLHHPFA"
     gene            complement(30933..32084)
                     /locus_tag="ACFM8S_000030"
     CDS             complement(30933..32084)
                     /locus_tag="ACFM8S_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HFC6313181.1"
                     /translation="MQTWQLPEHIADVLPTNARQLESAREQLLALFRVHGYELVQPPL
                     MEYAHSLLTHIDAGLSLKTILVTDRLSGRQLGIRADITPQVARIDAHLLSVNQGINRL
                     CYAGPVLHAQPDGLLNMREPLQAGAEMYGFADIRGDIELIDLMLKSMKIADMGKVLLS
                     LGHIGIFRALSDAAHLDAGQSATLLALMQDKDTGAVEAQVKAWKLDGMWAKAFSLLPR
                     LYGGREVLSDARGRLPDLSAVGGALGELQAVCDAFPDCEIHIDLSELRVDNYHTGLLY
                     AAYAADFHDAVARGGRYDGLGGYFGRARPATGFSFDLRSFIGRLPTIERQPAVLVDAE
                     DAEAAREAVEALREQGQCVVIDYGIGHNVSEELAGRLKKTDGVWQVVKR"
     gene            32419..33798
                     /gene="norM"
                     /locus_tag="ACFM8S_000031"
     CDS             32419..33798
                     /gene="norM"
                     /locus_tag="ACFM8S_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter NorM"
                     /protein_id="HFC6313182.1"
                     /translation="MLLDLNRFSFSVFLKEVRLLTALALPMLLAQVAQVGIGFIDTVM
                     AGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPMIAQLYGAGKTDEVGETGRQGIW
                     FGLFLGVFGMVLMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYA
                     SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGLATMAVFWFSALALW
                     IYIAKENFFRPFGLTAKFGKPDWAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFG
                     EDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRARYISGVSLVLGWML
                     AVITVLSLVLFRSPLVSMYNNDPAVLSIAATVLLFAGLFQPADFTQCIASYALRGYKV
                     TKVPMFIHAAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAIALVWCLELCSR
                     EMVRSHKAV"
     gene            33977..35017
                     /gene="murB"
                     /locus_tag="ACFM8S_000032"
     CDS             33977..35017
                     /gene="murB"
                     /locus_tag="ACFM8S_000032"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFC6313183.1"
                     /translation="MQPIRYRTDLTPYNTFGLRAQARAFIALEHADGLRDIVRLPEFN
                     RDTVLWLGGGSNILLMEDYAGLVVHMENKGIREIARSDGMVLIEAQAGEIWHDFVLHA
                     VALGLNGLENLSLIPGTVGASPVQNIGAYGVEAKDVIHSVRCFDLDTETFVTLSNADC
                     RFAYRESLFKQEGKGRYVIVSVVFALKTHFVPNLGYGDLAAAVAELSAGRVPTAKDVS
                     DAVCAIRNSKLPNPNVLGNVGSFFKNPVVSAEKAATLLQRHPDMPRYPQPDGSVKLAA
                     GWLIDQCRLKGFQIGGAAVHDRQALVLVNKNNASANDVRQLAQHIKFTVFARFQVELH
                     AEPNWLPASFSL"
     gene            35029..35679
                     /locus_tag="ACFM8S_000033"
     CDS             35029..35679
                     /locus_tag="ACFM8S_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFC6313184.1"
                     /translation="MPVTRPAKINTYTRIIDASLALFNEEGERNISTNHIAAHLGISP
                     GNLYYHFRNKDEIIVQLFKRYSEALLEYLNEAVLPSDVEDSINYMAGIYDVMWEYRFL
                     FSDVNTLLARSAELLGEHNTFTQAKVSPLLVNLLTQLNGLNIIQADQTAMNDLAVNMW
                     MITKYWFDFDSSLRGRTKLTEDSKARGIRRTLSLLRPYLLPEHRKKYDRKIGNGNP"
     gene            35734..36531
                     /locus_tag="ACFM8S_000034"
     CDS             35734..36531
                     /locus_tag="ACFM8S_000034"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC6313185.1"
                     /translation="MRAVPPPHISITGLGYLGLPLAQKFYQHGSRVAAIKRSLTSDDI
                     NLPIHLDTIDLNQDSAFQSANLARDTSFWRHHANKPVWFCLLPPSSLTHYADTVKQWA
                     ELARACNVQHLIFTSSTSVYGDTARECDEIALPDPQTESARQILAAEQHLLDSGISNI
                     DILRLGGLYCAERHPVSRLVQKQNIPGGNRPINIVHRNIAVESLFQTAFNPGGRRLKN
                     IIEPRHPTRREFYTEEAAKLGLPAPDFAPDDSVGKIIRTVCDNGLSL"
     gene            36582..37148
                     /locus_tag="ACFM8S_000035"
     CDS             36582..37148
                     /locus_tag="ACFM8S_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032934 - sterol binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFC6313186.1"
                     /translation="MSALLPIINRLILQSPDSRSELAAFAGKTLTLNIAGLKLAGRIT
                     EDGLLSAGNGFADTEITFRNSAVQKILQGGEPGAGDIGLEGDLILGIAVLSLLGSLRS
                     RASDELARIFGTQADIGSRAADIGHGIKQIGRNIAEQIGRFSREPESANIGNEALADC
                     LDEISRLRDGVERLNERLDRLERDIWID"
     gene            37168..38058
                     /locus_tag="ACFM8S_000036"
     CDS             37168..38058
                     /locus_tag="ACFM8S_000036"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFC6313187.1"
                     /translation="MNSPFHNIGIVTRPNTPDIQDTAHTLITFLKQHGFTVYLDEVGI
                     KEGCIYTQDTVGCHIVNKTELGQYCDLVAVLGGDGTFLSVAREIALRAVPIIGINQGH
                     LGFLTQIPREYMTDKLLPVLEGKYLAEERILIEAALIRKGKTAERAIALNDAVLSRGG
                     AGQMIEFEVFVNREFVYTQRSDGLIVSTPTGSTAYSLAAGGPIMQAGLHAFTLVPICP
                     QSMTNRPIAIPDTSEIEILVTQGGDARVHFDGQTHIDVQNLDRITIRRYRNPLRILHP
                     TDYQYFKTLRQKLHWGEQLV"
     gene            38083..38841
                     /locus_tag="ACFM8S_000037"
     CDS             38083..38841
                     /locus_tag="ACFM8S_000037"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFC6313188.1"
                     /translation="MNSKISSDHTEDAVRLSKRMAQLGLCSRREADGYIGQGWVTVNG
                     KTAVLGQKVSPADRIELNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITSE
                     NRWEGDTSRIRFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
                     VRVRGKLDEKGLALLNHGLSLDGEKLRPAQVEWQNEDQLRFVLKQGKKRQIRRMCELV
                     GLRVVGLKRIRMGKVKLGRLPPGKWRYLAPGESF"
     gene            complement(38896..39561)
                     /locus_tag="ACFM8S_000038"
     CDS             complement(38896..39561)
                     /locus_tag="ACFM8S_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFC6313189.1"
                     /translation="MTVLDREQVLSAFKNRKSCRHYDAARKISAEDFQFILELGRLSP
                     SSVGSEPWQFVVVQNPEIRQAIKPFSWGMADALDTASHLVVFLAKKNARFDSPFMLES
                     LKRRGVTEPDAMAKSLARYQAFQADDIKILDDSRALFDWCCRQTYIALGNMMTGAAMA
                     GIDSCPVEGFNYADMERVLSGQFGLFDAAEWGVSVAATFGYRVQEIATKARRPLEETV
                     IWA"
     gene            complement(39675..40448)
                     /gene="folE2"
                     /locus_tag="ACFM8S_000039"
     CDS             complement(39675..40448)
                     /gene="folE2"
                     /locus_tag="ACFM8S_000039"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HFC6313190.1"
                     /translation="MNTIADVQSSRDLRNLPINQVGIKDLRFPITLQTAEGIQSTIAR
                     LTMTVYLPAEQKGTHMSRFVALMEQHAEALDFAQLRKLTTEMVALLDSRAGKISVSFP
                     FFRKKTAPVSGIRSLLDYDVCLTGEIKDGAYGHSMKVMIPVTSLCPCSKEISQYGAHN
                     QRSHVTVSLTADAEVGIEEVIDYVEAQASCQLYGLLKRPDEKYVTEKAYENPKFVEDM
                     VRDVATSLIADKRIKSFVVESENFESIHNHSAYAYIAYP"
     gene            40640..41797
                     /locus_tag="ACFM8S_000040"
     CDS             40640..41797
                     /locus_tag="ACFM8S_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HFC6313191.1"
                     /translation="MKFATKAIHSSYDCDEHNRALMPPIYQNSMFALHEIGENVPYRY
                     SRLSNPTRQILEDTVADLEHGAAGFAFSSGMAGIDAVWRTFLRPGDTIVAVADIYGGA
                     YDLLVDVYQKWGVNVVFADLGNPDNLDELLKAHKVKLVWLETPSNPLLRLVDIKVLAA
                     KAKAAGALVGIDNTFATPYLQQPLDMGCDFVFHSATKYLCGHSDVLMGIVVAKTKELA
                     QPLHDMMVHTGAVAGPLDCWLVLRGIKTLALRMNAHCQNALEIARRLEAHPAIEKVFH
                     PGLPSHEHYELAKTQMPKGIGGVVTVYLKNDTREAANSVIKNMKLVKMASSLGGVESL
                     VNHCYSQSHSGVPHNVKMEMGIKVGLLRFSIGIEDADDIWNDISAALDTTL"
     gene            complement(41860..42861)
                     /gene="hemB"
                     /locus_tag="ACFM8S_000041"
     CDS             complement(41860..42861)
                     /gene="hemB"
                     /locus_tag="ACFM8S_000041"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFC6313192.1"
                     /translation="MQFPYRNVPASRMRRMRRDDFSRRLMREHTLTADDLIYPVFVLE
                     GSAREEDVPSMPGVKRQSLDRLLFTAEEAVKLGIPMLALFPVVTANKTERAEEAYNPE
                     GLVPAAVRALRERFPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALC
                     HAEAGAQVVAPSDMMDGRIGAIREALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSS
                     GNLGKADKKTYQMDPANTDEALHEVALDIQEGADMVMVKPGLPYLDVVRRVKDEFGVP
                     TYAYQVSGEYAMLQAAIANGWLDGGKVVLESLLAFKRAGADGILTYYAIEAAKMLKR"
     gene            complement(43044..43328)
                     /gene="yhbY"
                     /locus_tag="ACFM8S_000042"
     CDS             complement(43044..43328)
                     /gene="yhbY"
                     /locus_tag="ACFM8S_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFC6313193.1"
                     /translation="MTDTKLNTKEILELKARAHHLHPVVMVGQQGLTDAVIKETDAAL
                     TAHELIKVRVFGDDRAERIEICTALCEAVDAQPVQHIGKLLVLWRKNIEG"
     gene            43438..44058
                     /locus_tag="ACFM8S_000043"
     CDS             43438..44058
                     /locus_tag="ACFM8S_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HFC6313194.1"
                     /translation="MAVRSKSSKAWLHEHVNDHYVHMAQKDGYRARAAYKLLEINDKD
                     KLIKPGTVLADLGSAPGSWSQVAAKLTGSSGAVFALDILPMEAIGGVSFIQGDFREND
                     VLAQFETLLDNRPLDLVICDMAPNMSGNAVSDQVRSFYLCELALDFASQHLKTGGSFL
                     VKVFQGAGYQEYMAAMREIFGTVQTRKPEASRNRSSEIYLLGKNKR"
     gene            44122..46089
                     /gene="ftsH"
                     /locus_tag="ACFM8S_000044"
     CDS             44122..46089
                     /gene="ftsH"
                     /locus_tag="ACFM8S_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFC6313195.1"
                     /translation="MGNTFKSILVWVALGIGLMAAFNALDGKKEDNGQIEYSQFIQQV
                     NNGEVSGVNIEGSVVSGYLIKGERTDKSTFFTNAPLDDSLIKTLLDKNVRVKVTPEEK
                     PSALAALFYSLLPVLLLIGAWFYFMRMQTGGGGKGGAFSFGKSRARLLDKDANKVTFA
                     DVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILLAGSPGTGKTLLAKAIAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGN
                     DEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRPGRFDRQVVVPLPDIRG
                     REQILNVHSKKVPLDKSVDLLSLARGTPGFSGADLANLVNEAALFAGRRNKVKVDQSD
                     FEDAKDKIYMGPERRSMVMHEDEKRATAYHESGHAIVAESLPFTDPVHKVTIMPRGRA
                     LGLTWQLPERDRISMYKDQMLSQLSILFGGRIAEDIFVGRISTGASNDFERATQMARE
                     MVTRYGMSDKMGVMVYAENEGEVFLGRSVTRSQNISEKTQQDIDAEIRRILDEQYQVA
                     YKILDENRDKMETMCKALMEWETIDRDQVLEIMAGKQPSPPKDYSHNLRENADAAEDN
                     APHAPTREETEAPAPADTASTESEQQPENKA"
     gene            46311..46742
                     /locus_tag="ACFM8S_000045"
     CDS             46311..46742
                     /locus_tag="ACFM8S_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HFC6313196.1"
                     /translation="MKKVEKNILVLHGADKMFELVDKVEDYPNFLPWYSKTEVIGRNG
                     NELKARLFMDYMHVRQSFATHNRNIPGREIRMELLEGPFKTLRGTWKFIDLGDDMCKI
                     EFNLEYDFSNAVLSALISPVFNHLSATLVEAFVKEADRRYA"
     gene            46735..47013
                     /locus_tag="ACFM8S_000046"
     CDS             46735..47013
                     /locus_tag="ACFM8S_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFC6313197.1"
                     /translation="MLEIEIVYGLPDRQVLKTMQLAEGTTVRAAALQSGLDGIFEDLN
                     LHSAPLGIFGKAVKDDTPLRDGDRIEVYRPLLIDPKEARRKRVQNQEE"
     gene            47066..47644
                     /gene="pth"
                     /locus_tag="ACFM8S_000047"
     CDS             47066..47644
                     /gene="pth"
                     /locus_tag="ACFM8S_000047"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFC6313198.1"
                     /translation="MSNKIKMLVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEE
                     KKFFGEVARAALPDGDVWLLKPATFMNRSGQAVAALAQFYKIKPEEILVVHDELDIPC
                     GRIKFKLGGGNGGHNGLKDIQAKLGTADYYRLRLGIDHPGDRNLVVGYVLNKPSTEHR
                     RQIDDAVAKSLQAIPDILAGKWEEATRFLHSK"
     gene            47704..47910
                     /locus_tag="ACFM8S_000048"
     CDS             47704..47910
                     /locus_tag="ACFM8S_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HFC6313199.1"
                     /translation="MSELNRLIQTESVAVIEETLNFLLYECSIDDAPSAEEIAVWRDM
                     LAARGGKFLRLSKLCQTWLEEEQA"
     gene            47907..48269
                     /locus_tag="ACFM8S_000049"
     CDS             47907..48269
                     /locus_tag="ACFM8S_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFC6313200.1"
                     /translation="MNLPRNRFILLSALWFAGSIYSLLFKAAETAPPPFPHFDKVAHL
                     ALFFAQIWLLTKAFRTDNRPIPYRSLMVFALCFALFSECAQAWFTATRTGSLGDVLAD
                     LTGAALALFTARAACRPD"
     gene            48500..49915
                     /locus_tag="ACFM8S_000050"
     CDS             48500..49915
                     /locus_tag="ACFM8S_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFC6313201.1"
                     /translation="MNLHAKDKTQHPENVELLSAQKPITDFKGLLTTIISAVVCFGIY
                     HILPYSPDANKGIALLIFVAALWFTEAVHITVTALMVPILAVVLGFPDMDIKKAMADF
                     SNPIIYIFFGGFALATALHMQRLDRKIAVSLLRLSRGNMKVAVLMLFLVTAFLSMWIS
                     NTATAAMMLPLAMGMLSHLDQEKEHKTYVFLLLGIAYCASIGGLGTLVGSPPNLIAAK
                     ALNLDFVGWMKLGLPMMLLILPLMLLSLYVILKPNLNERVEIKAESIPWTLHRVIALL
                     IFLATAAAWIFSSKIKTAFGISNPDTVIALSAAVAVVVFGVAQWKEVARNTDWGVLML
                     FGGGISLSTLLKTSGASEALGQQVAATFSGAPAFLVILIVAAFIIFLTEFTSNTASAA
                     LLVPIFSGIAMQMGLPEQVLVFVIGIGASCAFMLPVATPPNAIVFGTGLIKQREMMNV
                     GILLNILCVVLVALWAYAVLM"
     gene            50034..50543
                     /locus_tag="ACFM8S_000051"
     CDS             50034..50543
                     /locus_tag="ACFM8S_000051"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC6313202.1"
                     /translation="MIILHTNKGDIKIELDFDKAPVTAKNFEQYVKDGFYDGVIFHRV
                     IKGFMIQGGGMDENMNEKETRDPIQNEASNGLPNDKYTIAMARTSDPHSASAQFFINT
                     ADNAFLNFRSKELYGKTVVQDWGYAVFGKVVDGFDVVDAIEGVSTKRHGYHDDVPSEP
                     VVIIKAEAV"
     gene            50710..52092
                     /locus_tag="ACFM8S_000052"
     CDS             50710..52092
                     /locus_tag="ACFM8S_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFC6313203.1"
                     /translation="MASIARDIFKAYDIRGIVGKTLTDEAAYLIGKAIAAKAAEKGIT
                     RIALGRDGRLSGPELMEHIRRGFTDSGINVLNVGMVATPMLYFAAVNECGGSGVMITG
                     SHNPPDYNGFKMMLGGDTLAGEAIQELLSIIEKDGFAAAGKQGSVTEKDISGEYLKHI
                     TGHIRLKRPMNIAIDAGNGVGGAFAGKLYKGLGNEVTELFCDVDGTFPNHHPDPSKPK
                     NLQDLIAALKNGDAEIGLAFDGDADRLGVVTKDGNIIYPDRQLMLFAQDVLNRNPGAK
                     VIFDVKSTRLLAPWIKEHGGKAIMEKTGHSFIKSAMKETGAPVAGEMSGHIFFKERWF
                     GFDDGLYAGARLLEILSASDNPSEVLNNLPQSISTPELNIALPEGSNGHQVIDELAAK
                     AEFEGATEIITIDGLRVEFPDGFGLMRASNTTPILVLRFEADTQEAIERIQNQFKAVI
                     ESNPNLIWPL"
     gene            complement(52112..52867)
                     /locus_tag="ACFM8S_000053"
     CDS             complement(52112..52867)
                     /locus_tag="ACFM8S_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFC6313204.1"
                     /translation="MIKIRNIHKTFGENTILRGIDLDVGKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEDGQIEFDNERPLKIDFSKKPSKHDILALRRKSGMVFQQYNLFPHKTAL
                     ENVMEGPVAVQGKPAAQAREEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLDTMKELAQEGWTMVVVTHEIKFALEVATTVVVMDG
                     GVIVEQGSPQDLFDHPKHERTRRFLSQIQSTKI"
     gene            complement(52877..53593)
                     /locus_tag="ACFM8S_000054"
     CDS             complement(52877..53593)
                     /locus_tag="ACFM8S_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFC6313205.1"
                     /translation="MFNNFLASLPFMTETRADMIVSAFLPMVKAGFAVSLPLAAASFV
                     IGMVIAVAVALVRIMPAGGIARKILLKLVEFYISVIRGTPLLVQLVIVFYGLPSVGIY
                     IDPIPAAIIGFSLNVGAYASETIRAAILSVPKGQWEAGFSIGMTYMQTFRRIVAPQAF
                     RVAVPPLSNEFIGLFKNTSLAAVVTVTELFRVAQETANRTYDFLPVYIEAALVYWCFC
                     KVLFLIQARLEKRFDRYVAK"
     gene            complement(53583..54410)
                     /locus_tag="ACFM8S_000055"
     CDS             complement(53583..54410)
                     /locus_tag="ACFM8S_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6313206.1"
                     /translation="MMLKKFVLGGIAALVLAACGGSEGGSGASSAPAQSAVSGSLIER
                     INNKGTVTVGTEGTYAPFTYHDKDGKLTGYDVEVTRAVAEKLGVKVEFKETQWDSMMA
                     GLKAGRFDVVANQVGLTSPERQATFDKSDPYSWSGAVLVARNDSNIKSIDDIKGVKTA
                     QSLTSNYGEKAKAAGADLVAVDGLAQSLTLIEQKRADATLNDELAVLDYLKKNPNAGV
                     KIVWSAPADEKVGSGLIVNKGNDEAVAKFSTAINELKADGTLKKLGEQFFGKDISVQ"
     gene            54695..55603
                     /locus_tag="ACFM8S_000056"
     CDS             54695..55603
                     /locus_tag="ACFM8S_000056"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HFC6313207.1"
                     /translation="MNTPQSAIIPDHAQAGIFIEADFAANRLNDIKAACRASLDALTA
                     LKARFPDDILGLTIAFGSKAWATFGHTDEGSEIKPFSEMGNGLAPSTQHDMSIHIQSF
                     RQNAAYALAQSVLGAFGDSICVASEEHGLRLYQDRGLDGFVDGTENPQGDENVREVAI
                     IPEGKPDAGGSYVLLQKYLHDLKKWDAVPVAEQEASVGRGKETDDEFGRDVRLPDSHL
                     GRVNLKENGVGLKIVRRSLPFGKISGEHGLMFTAYCRTLHNIEAQLLSMFGDTDGKTD
                     LLLRHLSAAVSGGYYYAPSVERLQNL"
BASE COUNT        13211 a        15240 c        13716 g        13478 t
ORIGIN      
        1 taatccacta tagattaaaa tcacacaata tccaactatt cggaccttca tatgacacat
       61 accgtccacc tgcatttaga agaaaccgac aacttggcgt tgcagcgtct gtgcggttct
      121 tttgacaaca accttgattt acttgctaaa gcactcgata tccacatcag ccgccgtttt
      181 gaacatttca ctttcaacgg cgcatttgca cacgccggca aacgcgcact gctcaaactc
      241 ttggaaacgg cgcagacgcg cgacctaaac gacggcgaca tcaggcttgc cgccgtcgaa
      301 gcccaaaccg aagatgccgg tcatcaagaa aaaaaccatg accacgccta ttatttccgc
      361 accaagcgcg gcagcatcgg cggcagaacg ccacggcaga acggctatat ccgcgcccta
      421 ctcaaccacg acatcgtatt cggtctcggg ccggcaggta cgggcaaaac ctatctcgcc
      481 gtcgccgccg ccgtcgatgc gatggaaaaa caccaagtcg aacgcatcat tttagtgcgg
      541 cctgccgtcg aagccggcga gaaactgggt tttctgcccg gagacctgac ccagaaagtc
      601 gatccctacc ttcgtccgct ttatgatgcc ctctatgacc tgatgggctt tgaccgtgtc
      661 accaagctga ttgaaaaagg cctgattgaa atcgccccgc tcgcctatat gcgcggcagg
      721 acgctcaacg gcgcatacat catcctcgac gaagcgcaaa acaccacgcc cgaacaaatg
      781 aaaatgttcc tgacccgcat cggcttcggc gcgaaagccg tcattaccgg cgacaccagc
      841 caaatcgacc tgcccaaaaa catcaaatcg ggattaaaag atgcgcgtga gaaactgcac
      901 aacgtggaag ggctgtattt ccacaccttt accggcgaag acgttgtccg gcatcctctg
      961 gtgcaaaaaa tcgtcgaagc ctacgaatcg gcagaacacg actgacattc caatgccgtc
     1021 tgaaacccga tgcatggaaa tcgggcagag aaaaacggat acggaaacca agctccgtat
     1081 ccgtttcttt atccgtccaa ccgatacatc aatcgaattt tctgcatcgg attgcctcat
     1141 cttcgcaaaa cattgccgaa gtttcgcggt tgcacgcctg acgcactttc tgccgcgcca
     1201 cgccttcatt ccgatcgctt gcctcaaacg tcttctgaaa aggcgtaacg ctgcatacat
     1261 acatggtgga atttgaaccg aaaaggtcga ttttgacggt attgctgccg ctttccaata
     1321 cggcgacacg gtgttccaac tgcgccagac gtgcttccaa atcgccgatt ctgtcggcaa
     1381 atgcaggcat ggcggcacac gcggtcaaaa tgcccaaaat acattttttc atcaattttt
     1441 ccttgtttct tgaaccctgc ccacccggac atcctttttc cgagaggcag ggttagactt
     1501 tatttacggg gatgaatccc cttacaaatc aggataatac atagaacggc actttatgct
     1561 tcatcttgtc tgttgaccct tattcccaat ccgttccctc aaccttcggc ttgggtttgc
     1621 gcctgcgacg gcggcgtttt ttcgcctgtc cttcgttttt ttcgtgtcgg gcggcggcct
     1681 cgtttttggt catctccgtc cgttgttcct gcgatgccgt ctgaaacgcc gtccaccact
     1741 cggcaagggc gcggtccgca ttgccggttt cggcgcgcaa gagcaggaaa tcataggcgg
     1801 cacggaaacg cgcctgtgca aacagtttgt gcggccttgc gcctttgcgg ttttcaaact
     1861 gcggctggaa catccaaatt tcgcgcatcg tggcggaaaa gcgttgcggt acgccccaac
     1921 cgcgttcgac ggtttcgcgc atcgtattga ttgcatcgga cagggcgggc acgggtttca
     1981 aaccctgttg cagattgctt ttccaatggc gttccaactc gggccacatc agggccgcca
     2041 gtacgaaacc gaccgaaacc gatttgtcgg cacgcagccg ctcatcggta tttttcaggg
     2101 caagcaccgt catttttccg gcgatgccgt ctgaaacgcg caaggcattg agcagcggat
     2161 ggatgtcgtc cggtatgtca aatccgttca aacgttttag acactcgcgc gcgtgccctg
     2221 aaaacagcag tttcataatt tcgtcgaaca gccttgccac aggttcgtgc ttcagacggc
     2281 atattgattc ggcaatcggt gcggcggttt cttccgacag atcaaatccc aatttgcccg
     2341 acaggcggat ggcgcgcaaa atcctgacag ggtcttcctg atagcgttcg gcggcatcgc
     2401 caatcataac cagcctgcgg gcggcaacat cggcaatccc gttgtggaaa tccaaaatct
     2461 cttctttttc cggatcgtaa tacaaggcat tgcaggtaaa atcgcgccgc atcgcatctt
     2521 cttcgatgct gccgtaggtg ttgtccttca tgattctgcc gtgcgcgttc tgatgaacct
     2581 tcgcaccgcc gcgaaacgtc gttacttcga taatctccgc accgttcatc acatggacga
     2641 tttgaaaacg cctgccgatg atgcggctgc ggcggaagag tttgtgcacc tgttcgggcg
     2701 tggcatcggt tgcgacatcg aaatctttgg gttcgatgcc gagcagcaga tccctgaccg
     2761 cgccgccgac cacataagcc tgaaaccccg cccctttcag acggcgtatg acgttttcgg
     2821 cggcaaagct caacatttcg gcacggatgt tgtgtctttc ggcaggaatg accgttttac
     2881 tttccgcttt tttactgctc cgaccggaag gcagcatctt attcaaccat tttttcagca
     2941 taaatattct aacccgaaga gcggcatctc cacgggcaac cgtgttcaga cggcatcagg
     3001 cggtacgatt ccgcaagatg ccgtaaccat aaacaaaagg cgtagaaact acgccgtccg
     3061 aatcacgccg ccctcgcggc ggcggcaacg cgccattata acagattgcc ctttaaaaaa
     3121 atcagaccct gcttttgtgg cggagtctga aatttgatgc accttatata ttgcgtccct
     3181 aagaagggat gattaacaaa aattaacgcc ctttactttc ttttccgatt gggaacattc
     3241 ccgtcgtaca aacaggcaaa tgaaggaaca gaaatcacat tcaaacggcg ttccgccgcc
     3301 tctacaccgc ttcccagtaa tcgatataca gctgcaattc gagattgttg cgccattcgt
     3361 tggcaacggg gcggtaaacc gtgcggatgt attcggggat gtcttcgctg caacgccaaa
     3421 acatggcttc aaattcgcag ccgtcttttt gcagccacgc ttttttgtgt ttaccctccg
     3481 cgcccaaagg ctgctggcgg acgacgtgaa attcgtcggt aaagcttggc ggcgcgaagc
     3541 cctgccccca aacgtgtcgg gcaaggtttt gcgcctgttc cagcgtgatg tcgcaggctt
     3601 tcaggctgcc gtcggtgatg aaggtttgcg acaaatcgtc ttcgcacacc atctcgcgca
     3661 cggcttcttc aaagaccgtc tgaaacgcgg gaatattgtg ttcaagtatg ctcaaacctg
     3721 ccgccatcgc gtgtccgccg aattttaaaa tcaaatcggg atggcgtttg gacaccaagt
     3781 ccaaagcatc gcgcaggtgc aggttgggaa tggaacgccc cgaaccgcgc acttcgccgt
     3841 tgtcggcagg cgcaaacacg atggtcggac gataaaaacg gtctttgagg cggctggcga
     3901 cgataccgac cacgccttga tggaagtcgt cgcgatacgc caccaaagtc gtctgacctg
     3961 aaggcagggt ttcaggaaag gcgttcagcg cgtcttgcag catagactgc tcgatttcgc
     4021 gacgctcgat attgaggttg tttaactgag ccgccagttc ctgagcttcg gaatcatctc
     4081 gcgccaacag gcaggcgatg ccgaccgaca tatcgtccag ccgtccggcg gcgttgatgc
     4141 gcgggcccaa cgcaaaaccc atatcaaacg gctgcgcctt gcgccagtcg cgccgcgcca
     4201 cttcaaacaa ggcgcggata ccggggcgca ttttgccgga gcgcatccgt ttcaaacctt
     4261 gcgacacgag gatgcggttg ttgtggtcga gagggacgac atcggcaacc gtgccgagtg
     4321 cgaccaaatc caaaagatcg cccagattcg gctctttcag gccgtctgaa aaataattgc
     4381 ggcggcgcag ttcggcacgc aacgccgtca acacataaaa aatcacgccc acgcccgcca
     4441 agcttttgct ttggaaaccg cagccttttt ggttcggatt gacgatgatg cagtcgggca
     4501 cggtatcggc aggcagatgg tggtcggtta caatcacatc caaacccaga gcctgcgccc
     4561 tcgccacgcc tgcgatgctg gcgataccgt tatccaccgt aatcagcaaa tccacgccct
     4621 gcgcggcagc gatttcggca agttcgggcg ttaagccgta gccgtgttca aagcggttgg
     4681 gcacaaggaa atccactttt gcccccatcg ccgccaaacc gtccaaaccg acggcacacg
     4741 ccgtcgcgcc gtcggcatcg tagtcggcga caatcaggat tttttccttg cgcccaaccg
     4801 catccgccaa acgtccggcg gcggcttcgc agtttgtcag cgtttgatac ggcaaaagcg
     4861 cagcaagctt gtcgtccaat tcggcaggac tttgcacacc gcgcgaagca caaagccggg
     4921 cgattaaagg atcggcaccg gcggtgagca aatgattaaa aacgtcggta ttgatggatc
     4981 gggtttggat tttgactgac ataacgtggg gttttactgt gcgtgtgtgc ttaaaatgcc
     5041 gtctgaaagc ttttcagacg gcattgctgt tcatttgcgg gcggcatcca cgaccgcacc
     5101 cagagccgcc gcaggcaaag ccagtaccgc gcccgccgca tccagtatca aaaagggggg
     5161 ggcgtatata agatatttgc aaacagcttg gatttgtcgg tatgtttttc agtaaccgtg
     5221 taataaatat cggcaggcac actttgctca aaatgataat cggcgttcag tttttgcggc
     5281 gtggcgtagt atttgccttt ggcggatacg cagcgcgtgt aaatagtccg attgtcaagt
     5341 tcgaccgctt caaactcaag ctgtttcagc ttggcgatgt cggcaggctt gtcggtatcg
     5401 tagcgcaggc aaaggccttc ggtactgaaa ttctggctgc caggcgattc gagtttgacc
     5461 ggcagggctt ggtggcgagc atagctcggg gtatcctcaa ctatttggaa gggtttgtcc
     5521 agccctgcct tcaaaatgcc cgtcagcttc gccgaatctt cgggattgac gacgaaccag
     5581 tatttcccgc ccatcatcac cagactgccc ttttccaatt gggcattgtc tttggcaacc
     5641 acaccgaagg cgcggatttg atccttgtca acgtgtttat aggcggtcgt ttggctgaac
     5701 gggtcgttca tgccccacat cattgccgta cagccgttca acatcaaggc tgccgccaaa
     5761 acggcggcgg tcgttttacg gaacaacatt ttatcttcca atcagaatga cggcggatgc
     5821 cgtctgaaaa tatgcctagg cttcccctgt tccgccttgg ctttccataa cggttttcaa
     5881 ctcttgaaac agggcgcgga aatttttcgg cggtttgttc tgttcctgtt cttttttggt
     5941 attgcggatg agcgtcctca gcttgcccgc atcggcgttt ggaaaatcgg acataaactg
     6001 cgtcagcgcg ccgtcgtccg ccagcagctc catccgcgtc cgctccacac gttgcaaaaa
     6061 ggcgttgtgc gccgcatcgt cgccgcgcag cttggcaaga aatgcctcga tgggcgcggg
     6121 atcggtatcg cgcatcagtc tgccgataaa ttgcgcctgc cgcttgagcg cgccgttgga
     6181 tgtgattttt ttataggtaa cgatggcttc gtacaaatct tcgtccaagc cgattttttt
     6241 caacgtgtcg cttgagagct tggtcagctc catacccaaa tcctgcaaac cgttcatctg
     6301 ctttttcatt tgggttttgc tgacccattc gtcttcttgt tcaaacatct tcattctcaa
     6361 attttatccg acgggcgcat tttaacagaa tgattccgcg ttcagacggc attttgcacc
     6421 gtccgccctg caaacggacg cttttgcccc tgccgcccga aacggttaaa atgccgcaac
     6481 gctccaccaa gaaagatttc tatgctgttc aaccacaccg cttccgaact tctcgacctc
     6541 tgccgccgca cgctcgactt ggcaaaagca gcgggcgcga ccgccgccga agccgatttc
     6601 agcgaatcca tcggacaaag cgtcagcgtg cggctgggcg aaatcgaaca aatcgagttc
     6661 cagcaggaca agtcgctgga cattaccgtt tacgtcggca aacgcaaagg ccgcgctggc
     6721 accgccgatt tctccgaaca agccctgcgc gacaccgtcc gcgccgccgc cgacatcgcc
     6781 cgccacaccg ccgaagacgg ttgcgccgga cttgccgatg cctgtctgat ggcaaaacac
     6841 atcggcgacc ccgaccttta ccacgaatgg gatttggata cggaagccgc cgtcgagttg
     6901 gcaaaacaat gcgaacaagc cgccctgaat gaggatgagc gcatcgaaaa ctccgaaggc
     6961 gcggcggtgc aaaccggcca ttaccaatac gtttacggca acacccacgg ttttgccgca
     7021 caccggcaag gcacgcacca cagcatttcc tgcagcgtcg ttgccgccga cgaaaacggg
     7081 atgcagcgcg actactggta cgattccgcc tgccgccatc cggatatgga cagccccgaa
     7141 accatcggtc aaaccgccgc ccgccgcact ttgcgccgac tcggcagccg cagcatcccg
     7201 acaggcagct accccgtcct cttcgatacc accgtttcgg gcggtcttat cggacacctc
     7261 gtcggcgcac tctccggcgg cgcgctctac cgccaaagca gtttcctgat cgacagcatc
     7321 ggcaaaaaag tcctgcccga tttcctcaac ctgcgcgaag aaccgcacat cccccgctct
     7381 ttccgcagca gctattttga tgcggaaggc gttgccaccg caccgcgctt cgtgattcaa
     7441 aacggcattg tcaaaggcta tttcctcagc agttacagcg cgcgcaaact cggtatgcag
     7501 accacgggca acgccggcgg cgcgcacaac ctgtatttga accatacgca cgaaacgcaa
     7561 tccgacctgc tgaaagaaat ggggacggga ttgttggtaa ccgaactgat gggacagggc
     7621 gcgaacacca ttaccggtga ctactcgcgc ggcgcggcgg gtttttgggt ggaaaacggc
     7681 gtgattgcct accccgtcca cgagattacc gtagccggac gcttgcagga tatgtaccgc
     7741 gacattgtcg gcgtggcgga tgacgctttg cggcgttcgt ccaacaaaat cggctcgatt
     7801 ctgattgcgg ggatgacggt tgccggaagc tgaacgccct gccgatgccg tctgaagacg
     7861 aaggcagcac gttttgcccc gtgcctgccc gtttgccgtt ttatgccgca ccgccttttg
     7921 ccgacaacat tgcacatttt ttcatcagca ctgattttcc cttatccgtc aaaatactaa
     7981 gccacttgtc cacaacaggg tacacacaag ttcaggataa ggcgtgcggg gattggggtt
     8041 tttccgccgc cggcaaaacg gcagacaaaa acggcgggac attgcctgtc ccgccgtttt
     8101 caaatataca tttgaccgta catcaggaat taagcagcct gaatgttggc ggcctgtttg
     8161 cctttagggc cggtggttac gtcgaaagag acgcgttggc cttctttcag ggttttgaaa
     8221 ccttccatat tgattgctga gaagtgagcg aacaaatctt cgccgccttc atcaggagtg
     8281 atgaaaccaa aacctttagc gtcgttaaac cattttacga taccggttgc cattagaaac
     8341 ttcctatact caaaaattaa caaaatcagc aaaacaaggc atacggcaaa ccgagccgtc
     8401 tggcgttcct tccagcttga tatactcggc ggatgcccaa tcctgcttaa ccgtcttttt
     8461 acatttgtta agacaaaacg tcaagctatg ttttcaaaaa agtgggaaaa taggcaaaag
     8521 taccgaaaat acaacaaaaa tgccgtctga acgcggcttt caaatgggac acactatgac
     8581 cgcgcaacac caatccgaca cgctcttgca ccgtctgaac accctgccgc cgaaacgtta
     8641 cggcgttttc ctattgaacg acgattacac cacgatggaa tttgtcgttg aaatcctgac
     8701 cgaaatcttc atgctcggac aagaacaggc ggtagcggta atgctcttgg ttcatcacga
     8761 aggcaaaggc ctgtgcggca cttacacgcg cgatattgcc caaaccaaac agcaacaagt
     8821 catgcagcgg gcaaaagccg aagggcatcc gctgcaatgt attgtcgagg agatttaata
     8881 tgcttgcacc cgaattggaa cagattttgc agcagcttta ccgcgaggcg cgtaaggctc
     8941 attatgaatt tatcagcctc gagcatctgc ttttggtact catcgaagaa gatgcctccg
     9001 tgccgaacgt cttaaaactc tgcggcgcgg atttgaaagt ggtgtccgaa cagctcgccg
     9061 ccagcgttgc cgaaaacacc cccctgattc ccgaacacct tttagacacg gtcgaaaccc
     9121 ggcccacgct cggcttccaa cgcgtgatgc aacgggcgat ggtgcatacc cagtctgccg
     9181 gaaaagccgc agtcgaaccg ttggacgttt tggtcgcgct gatgagcgaa accgacagcc
     9241 acaccgtcta tttcctcaag ctgcaatcgg ttacgcgttt tgaagttttg cgctgtattg
     9301 cccacggctc tcccgatgaa gatgaagacg atggcaacta ttcttcagac ggcatggacg
     9361 acgataatga aaaccgcacc aaaccgggca aaaacccttt atcggcgtac accgtcaacc
     9421 tcaacgccga agtcaaagcc ggccgtatcg accctttgat tggtcgcaaa cacgaaatgg
     9481 aacggctggt gcaaatccta tgccgccgcc gcaaaaacaa tccgcttttg gtcggcgaag
     9541 cgggcgtggg caaaaccgcg ctggcggaag gtttggcaca tcaaatcgtc aacggcggca
     9601 ttccagacgc gctcaaagat gccgaagtgt acgcgctgga tatgggctcg ctgttggcgg
     9661 gcacgaaata ccgcggcgac tttgaagcgc gggtcaaatc cgtcttgaaa cagctcgaaa
     9721 aaatcccgca cgccattttg tttatcgacg aaatccacac catcatcggc gcaggcagca
     9781 ccagcggcgg caccatggac gcgtccaacc tgctcaaacc cgcgctggca aaaggttcgc
     9841 tgcgctgcat cggcgcgacc acttacgacg aataccgcac cattttcgac aaagaccacg
     9901 ccttaagccg ccgcttccaa aaaatcgacg tggtcgaacc caccgtttcc gaaaccgtac
     9961 aaatcctgcg cggcttgaag ccgatgtttg aagccttcca ccaagtccgc tatacgcaag
    10021 gcgcactcga agccgccgcc gaactctccg cacgctacat caacgagcgt ttcctgcccg
    10081 acaaagccat cgacgtgatg gacgaagcag gcgcggcgca acggattctg cccaaatcca
    10141 aacagaaaaa agtcatcggc aaagcacaaa ttgaaaccgt catcgccaaa gttgcgcgga
    10201 ttcccgaaaa aaccgtgtcg cacgacgaca aacaagtgct gcaattcctc ggtcgcgatt
    10261 tgaaaaacat ggtttacggt caagaaaacg ccatcgacgc gctggttgcc gccgtcaaaa
    10321 tgtcgcgttc cggccttgcc ctgcccgaca aaccgatagg cagcttcctc ttctccggcc
    10381 cgaccggcgt gggcaaaacc gaagccgcca aacagcttgc ctactcgctg ggcgtaccgc
    10441 tgcaacgctt tgatatgtcc gaatacatgg aacgccatgc cgtatcgcgc ctcatcggcg
    10501 cgccaccggg ctacgtcggc tttgaacaag gcggcctttt gaccgaagcc atcaacaaac
    10561 agccgcattg cgtattgctc ttggacgaaa tcgaaaaagc ccaccccgac attttcaacg
    10621 tcctcctgca agtcatggac gcaggcaaac tgaccgacaa caacggcaag agtgccgatt
    10681 tccgcaacgt catcctaatt atgaccacta acgcaggtgc ggagagtctc agccgaccca
    10741 gcctcggctt taccgccaaa cgcgagcgcg gcgacgaaat gcaggctatc aacaagctct
    10801 tcacgcccga gttccgcaac cgcttggatg cgattatccc gtttgcgccc ttatccgaac
    10861 ccgtcatcac caaagtcgtg gacaaattcc tgctccagct cgaacaccgg ctcctcgaca
    10921 aaaaagtcga agccgaattc acgtcggcat tgcacaaata tctggcggaa aaaggttttg
    10981 acccgcaaat gggcgcgcgc ccgatgcacc gcctgattca ggaaaaaatc cgcaaaccgc
    11041 tcgccgacga actcctgttc ggcaaactat ccgacggcgg cttcgtacgg atagactggg
    11101 atgcggcaaa agaagaagcc gtgttgaagt ttaagaaaag caaggtcaaa ataaaaaccg
    11161 cctccgcata aaatcaaaaa tgccgtctga aatttcagac ggcattttgt caaatatcgg
    11221 tccctattcc ggcaaacttc tccacaaacg ccgcaatctt ttgaaaaaca ctttgtttct
    11281 tcgtcagata ttgcgggttg agcggactca ttttcggcag ggtttcggtc agttccgtgc
    11341 cgttttcgct ggcatagccg cgtttgagcg agccggtcaa atagcgtttg gcggcggttt
    11401 cgttcaggcc ttcggcggca atcaatcctg ccgcttcgtg ccgcatcacc tcttgcccgt
    11461 aggtgtaaaa ggtttccagt atggcgggaa cgtcgggtac tttgtccaaa tccgtatcgt
    11521 tgatgaaatc cacaatcaga ccctctttgg cgcggtgtcc gatacttgcg cggatgatgc
    11581 ggcggatttc ttccaccagc tccgctttgc ctttgatttt tttgtggtgt tcgaaaacca
    11641 gttgcaggat gtaatccaaa ttgatttcct gagatttgag caaatccacc tcaaaaacca
    11701 catcgtccca atcgattttc gattgctctt tctgttcgcc tgctttttgg cggcgcagcc
    11761 agtcgcggat gtcgttgtag gcggaacggt agtcctgcac cgccctttca gacggcatcg
    11821 gcacttgccg catttcctgc acgtcttcat cgctcaggta gtatttttcc tgaaacgcct
    11881 tcatcgcatc ttcgtccgcc gcatccacac tctgcaattc acgcagcgcg gcaaattcat
    11941 cgtagttctg caatacgttt tccgcccgca ggtattcgcc gaagagtttg gcaaaatctt
    12001 ttttgtcttt ttccgtttcg attttgtcgg gatcggggaa acgttggcgc aattcttttg
    12061 ccacatccag ataaccgcgc cgcgcttcgc cggtctggct gtcggtatag ccgttcatat
    12121 attcttcgta gcttttttcc agcactacat ttttggtgtt tttatcgccg aagagcgtga
    12181 tcgcatcaat ggttgcctgc tccaaatcgc ggaagcagac aatattgccg aaggttttgg
    12241 tagcatcata aatgcggttg gtgcgagaaa acgcctgcat caggccgtga tagcgcaggt
    12301 ttttatcgac gaacagcgta ttcagcgtcg gcgcgtcaaa acccgtcaaa aacatgccga
    12361 ccacaatcag caaatccact tcctggtttt tcacccgttt tgccaaatcg cggtagtagt
    12421 tctgaaaggc tttgctgtcc gtgccgaaat tggttttgaa acaggcgttg taatcgttga
    12481 tggcagcctg caaaaattct ttcgcgctgc tgtccatcgc ttccggttca aaggtctcgt
    12541 caacaatttc accgacggcg ttctgctctt cgttggccgc aaaggaaaaa atggtggcca
    12601 ctttcagcgg gtgcaggctg cctgcctgtt gtgttttgaa cgcttcataa tagcacttcg
    12661 ccgcatccac gctgctgacg gcaaacatgg cgttgaagcc tttgccgcct gcgttcagcc
    12721 ggtgcgtttt ctgcctgaac tggttcagga tatattgcgt gatttcgcgg atgcgttcgg
    12781 ggtgcagcag ggcttggcga ttttcggcgg cactcagttt cttctcgtcc tgttccgctt
    12841 ccacggcttt gaactgcggg cgcacatcgt tgtaatccac tttgaatttc aatacttttt
    12901 catcgcggat ggcatcggta atcacataag aatgcagctc ccgcccgaac acgcccgccg
    12961 tggtttccgc gcccaaagcg ttttcgggaa aaatcggcgt gccggtaaag ccgaactggc
    13021 agaatttttt aaatttcttt ttcaggtttt tttgcgcttc gccgaattgc gagcggtggc
    13081 attcgtcgaa aataaagaca acccgctgat ggtaaaccgg cagattatct tcactcttca
    13141 tcaggttgtt cagcttttgg atggtggtaa cgatgatttt gttgtcgtct ttttccaaat
    13201 tgcgtttcaa gcccgtcgtg ctttccgaac cgtttacgct gtcgggagaa aaacgctggt
    13261 attccttcat cgtctgatag tccaaatcct tcctgtccac cacgaagaaa accttgtcga
    13321 taaacgccga ttctgtcgcc agacgcgccg ccttaaagct ggtcagcgtt ttgccgctgc
    13381 ccgtggtatg ccaaacatag ccgccgcttt cgggcttact ccaattcttt gccagagccg
    13441 aactgttgat tttccacaaa atgcgttcgg cggcggcaat ctgatacggc cgcataatca
    13501 gcagcgtatt attcgcatca aacacgctgt aatgcagcaa aacgctcagc aatacgcttt
    13561 tctgcaggaa cgtggcggta aagtctttca aatccttaat cggatgattg tccgaccgcg
    13621 cccaattcat cgtgaaatcg aagctatttt tgtcgcgctt ggtggtgttg gcgaaatagc
    13681 gcgtgtccgt gccgttggaa atcacgaaga tttgcaggaa tttgaacagc gaattttcgc
    13741 tgttgaagct ctctttgctg taacggtgca cctgattgaa tgcctcgcgc accgccacac
    13801 cgcgcttttt caattcaatc tgcaccagcg gcaggccgtt taccaacacg gtaacgtcgt
    13861 aacggtttgc atgcgtgccc gtctgctcaa actggttgat aagctgcaca tggttgcggg
    13921 caaggttttt cttgtccagc agataaatgt ttttcagacg gccgtcatca aaagcgaaat
    13981 cgtaaatatg gtcgtcgtgg attttgcggg ttttatcggt aatgttttca gacggcctgt
    14041 ccagatattc cgtcaaaaac cgcgcccatt cgccgtctga aaacgccaca tcgttcagcc
    14101 gctgcaactg cgcgcgcagg ttttccagca gcctgctttg gctgttcaaa tccttgcggt
    14161 attcgtaacc ctgattctgc aaatcggcga ttaactccgc ttccaaccgg ttttccgatt
    14221 ggtagctgcc cgactgttcg attttttcat attggtcgag cacgatgaaa ttcggcgttt
    14281 cggcgatggg tttggtttcg aggttcatag ttcgtccttt gcagggtcgg gttttaaaca
    14341 gtttgatgtt cggattgggc tgcctgaaaa tgtgcaggct gctttggtcg gcttcggcgg
    14401 aatgcggggt ggttgtgtcc gccgtagggt gggcttcagc ccaccgcccc acctgccgtt
    14461 gttttactgt tgttgcagct tgctgctgtt tcggtgggct gaagcccacc ctactttgtc
    14521 tgcgacttct accccaccga tgccgtctga atcatcccaa tcgggttttt ctctttgttt
    14581 tgagcatcta tcgccatatt ttccggtttc cacacgctgc ggcggaatgc ggggcggttg
    14641 ccgctcaccg tagggtgggc ttcagcccac cgccccgcct gcccttgttt tactgttgtt
    14701 gcagattgcc gtttttttcg gtgggctgaa gcccacccta ccgaaaggca agcagctgtt
    14761 cgcggtaata ttcgtattgt ttgcggcgca gggcgatttc gtgcggcagg ccttcgctga
    14821 tggagtgggt cagggtatcg aatttgtcga ggatggcgac gattttttct tgttcgggga
    14881 gtggagggat ggggattggg aagcgtttaa aaccattcat atccacagaa gcaaaactcg
    14941 atgttgtcgt attctttttg caccattcac taagattaaa acaatagtaa taaagaaaat
    15001 aaatgctgag tttatcggca aattcaggct ttaaagataa actggtaaat ctttggttgg
    15061 ctaagaatgg aacggttatt agtgcatgct cacctatggt tgcagatgta gccatgataa
    15121 tagaattggc tggaaatagc ttaccgccct ttactgcgct ttttgatata tgctgtaatg
    15181 aattatctaa aatacgacca ttttccctaa tatcttccat tctaaaccaa gggattgagc
    15241 cattttccca atattcttta tttgatttgg aaggggtata gccattcttc aagtcaaaca
    15301 cctcccccaa cgtcttccaa accacatctt tcagacggca ttgatagcca tcagctatcc
    15361 cccccgattt gattgtcaaa atctaaaaga aagtcgcggt aataccggta ttggcgtttg
    15421 cgcagggcta attcagcttc cagctcggtg aatttgtcaa gtatttttac aattttttgt
    15481 tgggtttcca ggggtgggat agggattaat aattttttca agttactggc atttagagca
    15541 ggaatacctg taaatgtaca taaggcatga atctcttgtt caaattcaag taatatatga
    15601 tagagaaaac gaacatccaa ttctgttcta ttcttaatag taactgcata atttaagttg
    15661 ccacgaaaat atcttccttc ttgccacgtt attgagccga ctcccgctcc tcttgttgtg
    15721 atgccgatag ggtcattttc agtgttccat tcatcaatat agccaagcgg ctctttgcca
    15781 ctgttaataa ctggataact accaatatta tttgagattt tctgcttact tacagcttgt
    15841 cctgttttta tgatagctat cccattttct ccacccaacg gcttccactc caccggtgcc
    15901 gtctgaatca tctcaatcaa ttttttcgct ttgctttgca tatccatggt tgttttccgt
    15961 ttctttcccg ctgtggcggg atgtggggcg gttgtggggt gcttcggctc actctgtccg
    16021 tttgccgtta tttctgcggg ctgaagtccg tcctgctttc aggctgcctg caatggtgtg
    16081 caggctgctt tttcaggtag cctgaatggt agttattttg tgcggtcttt tttctgtttg
    16141 atctgttgca cggctgtttt ttccaacgct ttaacgcttt ccagataggc ttgctccacc
    16201 ggcgacagga tgcgcgcttc ataggcgcgg tattcgcgtt cggctttctg ctctgcctgt
    16261 ttgcggctga tgctgcctgc accctgtaat gtgccttgtc cgtacagccc ggaaaatttg
    16321 tccaattcgg ctatccagtc gcgcatatac atggggcttt gctcctgcgc tttgatttcc
    16381 gctaggtcga agaaggcgga aaccagacgg ttcaggcgga acagttcgtc ttcggtcaga
    16441 tagtttttgg cgattttggc ttcattcagc gtggggattg cgccttgaaa ggtggtcagc
    16501 cccataaagt ctttgctgct gtcggcacgg ctgtatatca gctcagctgc ggtttgccgg
    16561 ctggcggcat agtgcagttt gttttgaacg gcggcaaaaa aggtttggct ttcgctgctt
    16621 ttggggttgt agtcttggct ggtggcatat aaatcaagca cttgccggta tagggctttt
    16681 tcgctgctgc ggatgtcgcg aatgcggttg agcagttctt tccaatagtc gccgccgcct
    16741 gtgcctttca ggcgttcgtc gtctatggca aagcctttgg tcagatattc gtccagccgt
    16801 tcggttgccc attggcggaa ttggatgccc cgcgcggaac ggacgcggta gccgacggca
    16861 ataatcatgg gcagggaata atgggcgatt ttgcggttta ccttgcgccc gttttcattt
    16921 tgaactgtca actggaagtt gacagttgcc ttctcttcca attcttgctc tgcaagaatg
    16981 gttttaatgt gtttgctgat attttgtttg gtggtttggt acagttctgc catgtccgcc
    17041 tgcgtcagcc aaagctgtcc gccaaattcc tgcaaggcaa attgggcagt gccgtcgttg
    17101 gcggtgtata ggatgatgct catgcttcaa tctctgcaat cacttcgtca atttcacgcc
    17161 gcagccgttc gattttggca acggtttcgc tgatttcggc gttaagctgt ttgatgtcga
    17221 taatttcgcg ggtgtcttcg ggttcaacat agctgctgac ggcgaggttg tagccgttgt
    17281 ctttgacggt ttgctgggcg gcgttttggg cgatatgcgg cacatcggct ttatcggcga
    17341 agagtttgac gatttcggca atgtgttctt cggttaagac gttgttgttg gtttcttttt
    17401 taaagaagcc gcctgcgtcg atgaattgga tgtcggtatt gtctttgtgt ttggacaaaa
    17461 ccagtatatt gacggcgatg ccggtgccgt aaaagagatt gggcgcaagg gcgatgacgg
    17521 tttccacgta gttgccctcc accagatatt ggcggatttt ctgctctgcg ccgccgcgat
    17581 agaaaatgcc ggggaatgag acgatggcgg cgcggcctct gccggaaagg tagttcagtg
    17641 cgtgcagaat gaaggcaaaa tcggctttgg atttcggggc gagtacgcct gcaggggcaa
    17701 agcggtcgtc gttgattaag gtggggtcgc cgctgcctat ccagttgatg gaatagggcg
    17761 gattggaaac gacggcatca aagggtttgc tgtctttgag tttgggattg gtcagtgtgt
    17821 cgcccaattc gatgtggaat ttgttgtaat tgacgttgtg cagaaacata ttcatgcggg
    17881 cgaggttgta ggtggtgtgg ttgatttcct gcccgaagaa gccttcttcg atgatgtgct
    17941 cgtcaaactg ttttttcgcc tgcaagagca ggctgcccga gccgcaagct gggtcgtaga
    18001 ttttgtttac tttctcctgc ccgtgcaccg ccagccgcgc aatcagcttg gatacggata
    18061 cgctttgcgg ggtgaaaaat tcgccgccgg atttgcctgc gttggcagcg tagttggaaa
    18121 tcaggtattc gtaggcatcg ccgaaaaggt cgatgtggtg gtcttcaaaa ctgccgaaat
    18181 cgagttccgc cacgcctttt aggacggcgg caaggcgttt gttcttgtcg gcaacggtgc
    18241 tgccgagccg gctgctggtg gtgtcgaagt cgtcaaacag gcctttaatg tcttgttcgg
    18301 acggatagcc ggaggcggag ctttcaatcg cggtaaaaat ttctttcagc ttggtgttga
    18361 gctcttcgtt ttgatgggct tcggcggcaa tattgcaaaa aagctggccg gggtagatga
    18421 aatagccttt gactttgacg gcatcgtctt tgatttcggg cgtgatgatg ctgtccggca
    18481 tagcggcgta atcaatactg ctgtcgcctg cctgcatata gtcggtaaag ttttcgctga
    18541 taaagcggta gaaaagtgtg ccgagaacgt attgtttaaa gtcccagccg tccaccgcgc
    18601 cgcgtacttc gtcggcaatt ttccaaattt ggcggtgcag ttgggcgcgt tgttgcattt
    18661 cggtcatcat cgaaatccat ataaaagtta aacaaatcga aatcgcctga tattttcaga
    18721 cgatttttta cgggcattca aattaagcca aattttccag catccatttg acgtagcggt
    18781 cgacgcctga tttgacatcg aaaaattctt ccttatatcc ggcttcgcgc aatttggtga
    18841 tgtcggcttg ggtgaagctt tggtatttgc ctttgagcgc gtcgggaaat gggatgtagc
    18901 ggataaattc ttcttctacc aactctttta aactcatttc aggtttgcct tcggcggcgc
    18961 ggcaggcgtt gacggtggca gcggcgagtt cgttaaactg ttggctgcga cctgttccta
    19021 ggttgtagat gccggaaagt tcgggatggt cgaagaagta gaggttgact ttggcaacgt
    19081 cttcgacgct gacaaagtcg cgggtttgtt cgccgttgcc gtagccgtcg ttgctgccga
    19141 acaggttgac gtaaccgtgt tcgcggtatt ggtggaaatg gtggaaggcg acggatgcca
    19201 tacggccttt gtgttgttcg tgttgtccgt acacgttgaa gtagcggaag ccgacgactt
    19261 gggcggtcaa gccttctttc atgcggcggc gcaatacttg gtcaaacagg aatttggaat
    19321 agccgtacac attgagcggt ttttcgagtt cgcgctcttc acggaagatt tcgcctttac
    19381 catacacggc ggcactggag gcataaagga agggaatgcg ttcgtcctga caccagtcca
    19441 acaaatccag cgtgtactgg tagttgtttt ccatcatata caaaccgtcg tgattcatgg
    19501 tgtcggaaca cgcgccttga tggaaaacgg cttcgatgtt ttgataaggt aaaatgtgtt
    19561 ccctcacttg gcggatgaat tcatgtttgt cgagataatg ggcgatttcg cactcggcaa
    19621 ggtttttgaa tttttcgcct ttgctcaaat tatcgacggc aacaatgtca gtaataccgc
    19681 gttgattaag tgctttgacg atgttgctgc cgataaagcc ggccgcgcct gttacgatga
    19741 tggtcatatt cggtttcctt tgttttgagt aaaattaata aacttagtta caagaaatca
    19801 tcgatattgt cctgaaacac aaatttgcga tgatattcca aatatttttc tcttccttcg
    19861 ggatatattc tcatcttaat tttttgcatt tggattgata ttaagttttt cccatgtgta
    19921 acggcttagt cgagtaccgc aaatcaaacg accatcatcc aagaaagtaa tataaccttg
    19981 atcgaacaac caatcataag tcggcgttaa agctaaccca tttaaataat ctaatgcctc
    20041 tttctcattt ttctcagtaa tacaaaccat ataaggttta atatgactag cccttaacaa
    20101 aatctcatct gtaattaatg taaatggaca ttgcggcata tgatttataa catccttgcg
    20161 atatttctgc tgcccttttc tacttttctg tttaatgatt tcttgctcta cgaattgatt
    20221 aaattctgaa gccggtattt ctaatttttc tcttgataag agttgcgggt gcacgataga
    20281 gcgaaattga taatccaaaa atattcggaa ataaaataat ggttctgaat cttctatatc
    20341 tttgacaggc aatagtttaa gaattgataa ataactaatt ttgggcaata caatcttacg
    20401 ccaaattgac catatatcat cctccgaacg gatataaccc ctacggtaac ctttagaatt
    20461 ttccaaatgg tctgatacat catggattga aaagaaaata ttttctgtag gcagattatt
    20521 aatacttaaa acgtaatcat cataaaacct tgaaatatca tgatgatatt tctgttcttg
    20581 tttgtgatat tcaacttttg catcaaccaa ataacgctgt aaatttttct tattaaaaaa
    20641 acaatttttc tgaatgaaag gatattctac cctccttcca ttttttgtct cagtaatccc
    20701 ccaattttca tactcaaaaa atgtacttag ttttcgacca ctatcatcaa tataactacc
    20761 tacatatttg cgtgcttccc cattaccacc aaaaacagat aacttgttat tcctgtgaat
    20821 aaaactatct tccgcacgta aattttgaat actatcgaca ataaaatact ctatgttttc
    20881 aatagtaatt ctttgaaaat ccggcatttt actctccata aagcgtaata tccatttgtt
    20941 ttaatatagc gattaataca tcaacaacaa tactgtttcc cgcttggcga tacatttgag
    21001 tatcactaac taaaatttta aaatcatctc taaaacccat tagccgcaaa cattccctag
    21061 gtgttaattt acgaatacgt ccacgattat gagtaacata attatcaacc ccggcacgat
    21121 gcattttatg catagtttgc aataatgggc gagctacagg caaatcagtt tccgtactgg
    21181 ttttaaaatt ttttgttcct cctgccaaaa cataattttt gattttttca gaaagataat
    21241 atttttcctc aacgttattt acatcaaaaa taaagtcatc aaattcagat tcgcgggctg
    21301 cctgaaaaat aaaatcacca tgccaattaa attgttgatt tgctttttgg cataaggcaa
    21361 tttctccatt aatctgtgta taacgttttt gtctattttt agaactggta acaaatttca
    21421 cacccttttc acgtaaaaaa tatttgctat cagtatagtc ctccaaaaaa tcttgcatag
    21481 tatgttctaa ttcaatcttt tcaggaaact gaaaaccatt tataggaggg gtatgaaagc
    21541 caacaacaaa aatacgctca cgatgttgag gaatcccata atccttacta ttcattattt
    21601 ggaaatataa gtcataacca agtgaataaa aaacactttt tacaactttc caagtttttc
    21661 cattatcatg attgagcaag ccctttacat tttcataaat aaatatcttt ggttggactt
    21721 catccacaac gcgagcaaat tcatagaata atgttcctcg tgtatcttct aatcctgcac
    21781 gtttgccaac catggaaaat gcttggcaag gactgcctcc aactaaaata tcaacttgat
    21841 ttctaaactt tctcgcatca aattgagtaa tgtcgttatg ccaaaaatct tcatttaatt
    21901 tatagtttcc aagataactt tttttaacgt atggatcaat atctcctgca aaaacaatgg
    21961 tatggtttaa atttaatcgg tggaatgcct gttcaaccgc accaatcccg ctgaatacgg
    22021 ttgctaatct aatatgtgaa tcaggtttaa gaaaagtttt agatttccaa ccttgttgac
    22081 tgggaaagag caaagttttt tgtaatcgag tatcgtgtgt ctgtgccatt gtcgaaatag
    22141 tcatacttat atcgttctgt ttatcttatc aatatgaaaa ctacattgtt gattgccctg
    22201 acaatgcctt gatcaattcg gcaaacgagc aaaccgccgt accgagtttc gccacgacaa
    22261 ccccggccgc agtattggca aggtacatgg cttcgggcat ggtgcagcct gccgccaaac
    22321 ccaagcccat tccggcaatg acggtgtcgc ccgcaccgga tacgtcgtaa acttcttggg
    22381 cgcgggtggg ctggtaaatc ggttcgcctt cgctgaacaa ggtcatgcct tcttcgcttc
    22441 gggtcagtaa aacggcggtc aggtcgaggt ggcggcgcag gttttgcgct ttttcggtca
    22501 gctcgctttc gtttttccaa ctgccgacca cttctttcaa ttcggcgcgg ttaggcgtaa
    22561 tcagagttgc accgacatat ttttcgtaat cgtcgccttt ggggtcgatt aagacggttt
    22621 tgccggcgtg tttcgcccaa tcgatcatat cggagatatg cgacaggccg cctttgccgt
    22681 agtctgaaaa aatgattgcg tcgtattcgg gcaagatttc gcggtatttc tgcttgattt
    22741 gttccaacac ttcgcagttg ggatgttctt caaaatcaag acggataagc tgctggttgc
    22801 gggcgacgac gcgcagtttg acggtggtgg cgatttgttt gtcgcgcatc agataggagg
    22861 cgacgccgtc ctgcaccatc agcgcatcga gcgcgtcggc ggcttcgtcg ttgccggtta
    22921 cggacaacag ccctgccctg ccgcccaacg aagcgatgtt gcgcgcgaca tttgccgctc
    22981 cgcccgcgcg ttggtcgatt cgtccgattt tcgccaccgg cacgggggct tcgggcgaaa
    23041 tacgggacac atcgccgaac caatagcggt cgagcatcac gtcgccgaca accaggactt
    23101 tggcttgcgc gaaacgggat ttgagggttt cttgttggaa cttggcggac atattgcctc
    23161 cgttgtattt tttcagacgg cctcagcctg taaggccgtc tgaaaatcag tttgcttcaa
    23221 ggtttgccac gcggttcact tcgcgcaata cggctaccgg atcggcagct tgggttacag
    23281 gacgacccat taccaaataa gtcgaagcgg cagccaaggc ttcggccggt gtcatgatgc
    23341 ggcgctggtc atcattattg ccggcaacgt ccaagcggat gccgggcgtg accaagacaa
    23401 aatcttgtcc caactcgcgg cgcagcggtg cggcttcttg ggcggaacag accacgccgt
    23461 ccaagcccga actttgcgcc agttttgcca agcggatgac ttgttcttca ggggcggtgt
    23521 tcaaaccgat ttccgccaaa tcgctttgtt ccatgctggt caacacggtt acgccgatta
    23581 agagcggctt tgtaccgtat ccggcaacgg cttctgctgc ggcttccatc atacggcggc
    23641 cgcccgatgc gtgcatatcg accatccaaa cgcccatatc ggcagcgact ttgcaggctt
    23701 gcgcgacggt gtggggaata tcgtggtatt tcaaatcgag gaaaagtttg aaaccttgat
    23761 tgattaagct ttctgccaaa ttgcgtcccg tcgcggtaaa cagctctttg ccgattttga
    23821 tttgacacaa tgtcgggtca aggttgcgga cgaatccgag cgtgtctttt tcgttggaaa
    23881 aatcaagggc gacgatgacg ggggtgcgtt gttgcggagt ttggaagtcg gagattaaag
    23941 gattcatggt gttttcgttt cgggttttca gacgaccttt atcgtctttt tcgcagtata
    24001 gtggattaac tttaaaccgg tacggcgttg tctcgcctta gttcaaagag aacgcttctc
    24061 taaggtgctg aagcacaaag tgaatcggtt ccgtactgtt tgtactgcct gcggcttcgt
    24121 cgccttgtcc tgatttttgt taatccacta taacaacgta aattcaaacc gtaggtcggg
    24181 cattgatgtc cgacctacgc ggactcaagc ctccgacaaa ctgtttcgga taaatttgaa
    24241 ccggtacatc agccgtcatt cccgcgcagg cgggaatcca ttgataaaac tcagttcatc
    24301 agatttaaac agcgattgcc caaattcaga aatggattcc cgcctgcgcg ggaatgacgc
    24361 caaatgatta tttttcggtt gcaacacgct gtttatcaaa aacaggtcgt ctgaaaacct
    24421 gattttctgt tttcagagtg cgaagcctgc ccgtgggtac gaccttttcg atttatttgc
    24481 ccaatgcgcc caatacttcg gctttcacgg cttctaccgg ctgagtgccg tcaactttga
    24541 tgtatttagg cgcgtgttcg ccttccagtt tgctgtaaaa atcgaccaaa acttcggttt
    24601 gctcgtggta aacggcaagg cgttttttca cggtttcttc tttgtcgtcg tcgcgctgaa
    24661 tcaaatcttc gccggttacg tcgtctttgc cttcaacttt gggcgggttg taggtaacgt
    24721 ggtaagtacg gccggaagcc aaatgcacgc ggcggccgct catgcggtcg acaatcacgc
    24781 tgtcaggcac atcgatttca acgactgcat ccaaatccac gcctgcttca accatcgctt
    24841 cggcttgtgc caatgtgcgc gggaaaccgt caaacaggaa accgtttttg cagtcgtctt
    24901 gcgcgatgcg ttctttgacc atgccgataa tgatgtcgtc gcgcaccaag ccgccttcgt
    24961 caatgatttt tttcgcttcc aaacccaagg gcgtgcctgc cttaatcgcg gcacggagca
    25021 tgtcgccggt agagatttgc ggaatgccga acgctgcggt gatgaattgc gcctgagtgc
    25081 ctttgcccgc gcccggtgcg cctaaaagta atgctttcat gaagtgatcc ttttaaatgt
    25141 gtttattgga atcaaacaga tggtttacga ttctacacgg cttggcacaa tagttcaaaa
    25201 accaactgca caaactgcca tttgcaagca aatgttttgc atacctttgc cgagcctgat
    25261 attcacaagg cggcaacttt ttgggacaat ccccaaaatt tcaaaacatc acaccctacc
    25321 aaaaaggaac atccgtgaaa cgcatttttc tgtttttggc taccaatatc gctgttttgg
    25381 tcgtaatcaa cattgttttg gcggttctgg gcatcaacag ccggggcggc gcgggcagcc
    25441 tgttggcgta ttccgccgtc gtcggcttca gcggctcgat tatttcgctg ctgatgtcca
    25501 aattcatcgc caaacaatcg gtcggtgcgg aagtcatcga cacgccgcgc accgaagaag
    25561 aagcctggct tttgaacact gtcgaagccc aagcgcggca atggaacctg aaaacgcccg
    25621 aagtcgccat ctaccactcc cccgaaccca atgcctttgc cacgggcgca tcgagaaaca
    25681 gctccctgat cgccgtcagc accggtttgc tcgaccatat gacgcgtgac gaagtggaag
    25741 ccgtattggc gcacgaaatg gcacacgtcg gcaacggcga tatggttacg ctgacgctga
    25801 ttcaaggcgt ggtcaatacc tttgtcgtgt tcctgtcgcg cattattgcc aacctgattg
    25861 cccgaaacaa cgacggcagc cagtcccagg gaacttattt cctggtcagc atggtattcc
    25921 aaatcctgtt cggcttcctt gccagcttaa ttgtcatgtg gttcagccga caacgcgaat
    25981 accgcgccga cgcgggcgcg gcaaaactgg tcggcgcgcc gaaaatgatt tccgccctgc
    26041 aaaggcttaa aggcaacccg gtcgatttgc ccgaagaaat gaacgcaatg ggcatcgccg
    26101 gagatacgcg cgactccctg ctcagcaccc acccttcgct ggacaaccga atcgcccgcc
    26161 tcaaatcgct ttaaaccgat ttgaaacggc aaaaaccgta cgcgcaagca gtacggtttt
    26221 ttgtatatgc tgtctgaaaa catcaggttt ccataccgac accaaccgta tccatttccg
    26281 caccggatac gccgcattga tataccctga taaaccgtat ccggacagaa aatatagtgg
    26341 attaacaaaa accagtacgg cgttgcctcg ccttagctca aagaaaacga ttctctaagg
    26401 tgctgaagca ccaagtgaat cggttccgta ctatttatac tgtctgcggc ttcgtcgcct
    26461 tgtcctgatt tttgttaatc ccctatatca gataaaacag cggtaaaatt gccgtctgaa
    26521 accaactctg cgccatacgg gacacgctct gcacgtctat ttgcctccga tatatcctcc
    26581 ctactgtagg aacgtctgcc gatttgcacg aaatcggcac ctctagcacc cttcaggatg
    26641 gcggacgtta tccgttcttg ggattctgca agacggtcga cggcattata cagacgctcg
    26701 gtctgatacc cttccgaagc aacctgcttc aggtattcca aataaccgta acccaacgcc
    26761 cctccgacaa gcagggtcgt caacgctacc agcgttgccg aacggatttt agggggcata
    26821 tcttgaagca tatctctacc gaaccgaacc aaagtctctt taaagtcgga ctctacacgc
    26881 gtagaaccct cggaaatctt gaagttcaac tcatactgct tccgtttgtc cgctgtcaac
    26941 cgcttgaggt tgtcatcccc gtattggacg gttgacacaa ccctgtacag actgtcctgt
    27001 atttcgacca gcgtctgtgc aaagttggcg gtcataactg gattcccaac ccttgccgcc
    27061 gattctgata ctcaaaacgg tatccaggcg ttccagcagg gcttcgggag acagcagtgc
    27121 gtattcttca atattttcca taggttgatt atagcagaac cttatacctg ttacagacaa
    27181 ggttgtcggg atttgaacgt ccgtcgagct tggttcgtat agagccgtag ggggtgttta
    27241 gggctttggc ggcagcttgt acgctgggga acgtgagtgt ttcgcctgtg gcggtgtcga
    27301 ggatggagac aggtttttgg ttttggggag gcgtattttc cttcatttta agtttcccgc
    27361 actccggaca cccatacttt gactttatag ctgtgtcgta atacaaaagt tggacaaccc
    27421 cgtgcgtagg acatctaaca ctggaaggtt tataaatgcc actgaactcc aacaattcta
    27481 aatgtggaaa tttctcttga aagcgtgatt tggcggtatc gaaagtcatt cgcttaccca
    27541 taatttgtct ccataggtta tgtgcaaatg gattacacaa ccaaaatata gaaattttca
    27601 aattttttga gacctacctg aaatcctgcg caagcacaat gtaaagaaaa atggtatacg
    27661 tctttactaa atctttacat aaattaactc taaccgggat ttgaacgtcc gtcgagcttg
    27721 gttcgtagga tttagggtgt tgtgaacctt acctatcgta cccaagcact ttgtacctgt
    27781 ttgaaataag gttgtcgggc gacgttcgac ccttgagcct gtgattgatt tgttggaaat
    27841 gtacacctaa agcagcacct gcggcagtaa cgcttgggaa cgtgagtgtt tcgttggtcg
    27901 ttgtgtcgag gatgaggagg ggcttcccta catttggagg tgtttgggtt tttgacatct
    27961 tagcacactc aggacacccg tactttgact ttataagggc attaaatgtc gaataacgaa
    28021 cgttcccgtg cataggacaa ttaacggtta ctgggtaatt cacaccgtca aagtcggtta
    28081 gagagatatt agggaatata tcagtaagtt cagaagctgc ttggttgaaa gatttggcca
    28141 tttgagactc caaaattgat tgtcaaatgg atttaatagc aaatttataa aaatgtcaaa
    28201 tttttcgaga agcacctgat agtggcgagg ggtatacact gttaaaaagt gtatacgtca
    28261 tttagtgcag atgtgaacct tatacctccc tgcaagtaaa gtctctgtgt gaacccgacc
    28321 ttttatacgg tcagtgatag ctgctgggtt tatacccaat gaccttgcag cagatgctct
    28381 tgaggggaat gtaagtgttt cgccagttac agtatcctcc aatactgtat ttttagataa
    28441 tttatctatt gtatcttctg gaattttgtg ggaattgaca ccataagatg cacattttgg
    28501 acacccactt ttagagtcca acatactttt gaatgtagag caagtaaccc tcccgtgtat
    28561 aggacagacg attaccgtcg ggtaacgaac gccattgaac tccacaagat tcaaatgtgg
    28621 aaatttagag gttagcttag aaactgcttg agtaaatgta agtgccatag tggttgtgaa
    28681 cttactttac caacctataa cgatacctaa tcaatcggtc aaacggagtg cgtccgctta
    28741 aacgtacagc taaagtgctg ttggagcaac cgataaattt actggcagcc agtgtacttg
    28801 caaactcgta gatttcccct gtctctgtgt cctccaacct aatggcaacg gcgtttggag
    28861 agggcttttc gggatttcga gtacattcag gacatcctgt tgaggatttc atggcagctt
    28921 gtagattccc gtaaacaacc gacccatgca aagggcattg gattgttacc ggttcgtagt
    28981 ttttagtgaa cttcaaaacc gtcttatcag gaaatttact ggaaaacctt aactgaacat
    29041 cttgtagcga gagtgatttt ggcattatag acctttctaa aatgaaagca ttatacaacc
    29101 aaaatataga aattttcaaa ttttctctga acgcactggt atccgatgga gctaaatgta
    29161 aagaaaaatg gtatacgtct ttactaaatc tttacacatt ttaactgtat agtgtttgac
    29221 aaacaacagg cataggcgca taatgcaccc gtagttagat agatagtaag gagataacca
    29281 atgacttagc acctgccccc ctctctctcg cgcaccccgc ccctcgcttg cggctaatgg
    29341 gctttgcgcg acatatacac tttcatctcc tgcatggctt aactgcctat gcaatagtac
    29401 acacatcaac aaatcaggct ggcacgtctt ggcattggct gacgtaccat tgtagcccga
    29461 aatgttgata ccctactatt tatgaccaac ggcggattag ggcttcagac ggcaatttta
    29521 ctgcaaaacc ttatgcgaac ggatgatgca gcacaatcgt ttcttcgcgg tcggggccgg
    29581 tggagacgat ggcgaccggc gcaccgcaga cttcttcaat ccgtttcaaa tatgctttgg
    29641 cattggcagg taatgcgtcg tagcttttca cgccgaaagt ggattcgctc caaccgggca
    29701 tggtttcgta aatcggcttg caggcttcta ccgcatcaga gccgcaaggc aggatgtcgg
    29761 ttttgccgcc gtcgggcaat tcatagccga cgcagatatt gatggtttca acgccgtcca
    29821 ttacatcgag tttagtaata cacataccgg aaatgccgtt gatttggatg gagcgtttca
    29881 atgcggcggc atcaaaccag ccgcagcggc gtgcgcgtcc ggttaccgaa ccgaattcgt
    29941 gtccgcgttc tgccaaacct gcgccgactt cgtcgaacaa ttcggtcggg aacgggcccg
    30001 aaccgacgcg cgtggtatag gctttgacga tgcccaaaac ataatccagc atttgaggac
    30061 ctacgcccgc gcctgcggaa gcggctcccg ccagacagtt ggacgaggta acgaagggat
    30121 aagtgccgta gtcgatgtcc aacaacgcac cttgcgcgcc ttcaaacagc agtttttcgc
    30181 cgtttttgtt tttctcgttc aacacgcggg acacgtcggt aatcatcggc gcaatgcgcg
    30241 gcgcgacttt ttcgataacc gccatcacgt cttccgcttt aaccggctcg gcattgtgca
    30301 gatgttgcag ttggacattg taataggcaa ggacggcatc cagtttttca cgcagttttt
    30361 caggatgcag caaatcggcg gcgcgaatgg cgcggcgtgc cactttgtct tcgtaggcag
    30421 ggccgatgcc gcggccggtc gtgccgattt tgcctttgcc gcgcgatgct tcgcgggctt
    30481 ggtcgagcgc gatgtggtaa ggcaggatca gcgggcaggt cggcgcgatt ttcagacggc
    30541 cttcgacgtt tttcacgcct gccgcgttca actcgtcgat ttcgcccaac agggcttcgg
    30601 gggagacgac aacgcccgaa ccgatgaagc agtccaaact ttcatgcagg atgccgctcg
    30661 gaatcaggcg caaaatggtt tttttgccgc cgacaaccaa ggtatggccc gcattgtggc
    30721 cgccttggaa gcgcaccacg ccgccggctt cttccgccag ccagtcaacg attttacctt
    30781 taccctcgtc gccccactgt gcgccgatta ctacaacatt tttagccata gccatataac
    30841 ctatcgatat taaaaattat tgcggaaatc cccaaaccgg tgcggctttg cgccccgcca
    30901 cgatttgcct ttcatccgcc atgaacgggt atttagcgtt tcacgacctg ccaaacgccg
    30961 tccgtctttt tcagacggcc tgcaagctct tccgaaacat tgtgtccgat accgtagtca
    31021 atcacgacac actgcccttg ttcacgcaag gcttcgactg cttcgcgcgc cgcttcggca
    31081 tcttccgcat cgaccaacac ggcgggctgc cgttcgatgg tgggcaaacg cccgataaag
    31141 ctgcgcaagt cgaaactgaa tcccgttgcc gggcgcgccc tgccgaaata tccgcccaat
    31201 ccgtcataac gcccgccgcg cgcgaccgcg tcgtggaaat cggcggcata ggcggcatac
    31261 agcaagcccg tgtggtaatt gtcgacacgc agctcggaca agtcgatatg gatttcacaa
    31321 tcggggaatg cgtcgcacac cgcctgcaat tcgcccaacg cgccgccgac cgccgacaaa
    31381 tccggcaacc gtccgcgcgc gtcggacaac acttcacgcc cgccgtacag gcgcggcaac
    31441 agcgagaatg cttttgccca catgccgtcc agcttccaag ccttgacctg cgcttcgacc
    31501 gccccggtat ctttatcctg catcaaggca agcagcgttg cggactgccc cgcatccaaa
    31561 tgtgccgcat cggacaaggc gcgaaatatg ccgatatgcc ccagcgaaag cagcactttg
    31621 cccatatcgg caattttcat gcttttcagc ataaggtcta tcagctcgat gtcgccacgg
    31681 atgtcggcaa aaccgtacat ttccgcccct gcctgcaagg gttcgcgcat attcagcaga
    31741 ccgtcgggct gcgcgtgcaa caccggaccg gcataacaca accgattaat cccttggttg
    31801 acggataaaa gatgggcatc gatacgcgcc acctgcggcg tgatgtcggc gcgtatgccc
    31861 aactgcctgc cgctgagcct gtccgttacc aaaatggttt tcagggaaag ccccgcatcg
    31921 atatgcgtca gcagggaatg tgcgtactcc atcagcggag gctgtaccag ttcataaccg
    31981 tgtacgcgga acagtgccaa caactgctcc ctcgcgcttt caagctgccg cgcgttcgtg
    32041 ggcagtacgt cggcgatatg ttccggaagc tgccatgtct gcatgagaat ctgccttcta
    32101 ttttatcatg taaatacaaa aggctgcccg atccgcaatc agacggcatc tttgccgcaa
    32161 cgcatcacgc cgctgtcaaa acggcgatgc ttccttccga ttcagaatcc gaacagcccc
    32221 tgtccgacgg ctcaatgccg tcaagtcgtt aaaaccaaac tttaccataa aatacacaca
    32281 atctgaatat acttttatac cctgtatccg ccgtctgacg gcacggcatt tttttactat
    32341 ataatgtgca ccattatttc tcatcacttc ctgctgccgt tttctgcttc agacggcatt
    32401 tttgttgatt gaacacttat gctgctcgac ctcaaccgct tttccttttc cgtcttcctg
    32461 aaagaagtcc gcctgctgac cgctcttgcc ctgcccatgc tgttggcgca ggtcgcgcag
    32521 gtgggcatcg gttttatcga tactgtgatg gcgggtggtg caggcaagga agacttggcg
    32581 gcggtggctt tgggcagcag cgcgtttgcc acggtttata ttacctttat gggcattatg
    32641 gcggcgctga acccgatgat tgcccagctt tacggcgcgg gtaaaaccga cgaagtgggc
    32701 gaaacggggc ggcaggggat ttggttcggg ctgtttttgg gcgtgttcgg catggtcttg
    32761 atgtgggcgg cgattacgcc gttccgcaac tggctgacct tgagcgatta tgtggaaggc
    32821 acaatggcgc agtatatgct gttcaccagc ttggcgatgc cggcggcaat ggtacaccgc
    32881 gcactgcacg cctacgcctc cagcctgaac cgcccgcgcc tgattatgtt ggtcagcttt
    32941 gcggcgtttg tgttgaacgt gccgctgaac tatattttcg tttacggcaa attcggtatg
    33001 cccgctttgg gcggcgcagg ctgcggactg gcgacgatgg cggtgttttg gttcagcgcg
    33061 ctggcattgt ggatttatat cgccaaggaa aatttcttcc gcccattcgg actgacggcg
    33121 aaattcggca aaccggattg ggcggtgttc aaacagattt ggaaaatcgg cgcacccatc
    33181 gggctgtctt attttttgga agccagcgcg ttttcgttta tcgtgttttt gattgcgcct
    33241 ttcggcgagg attatgtggc ggcgcagcag gtcggcatca gtttgtcggg gattctctat
    33301 atgattccgc aaagcgtcgg ctcggcgggg acggtgcgca tcggcttttc gcttgggcgg
    33361 cgcgaatttt cgcgggcgcg ttatatttcg ggcgtgtcac tggtgttagg atggatgctc
    33421 gccgtgatta ccgtgctttc cttggtatta ttccgttcgc cgctggtaag tatgtacaac
    33481 aatgatccgg cggttttaag catcgccgcc accgtcttac tgttcgccgg cttgttccaa
    33541 ccggcagact tcacccaatg tatcgcctcc tacgccttgc gcggctacaa agttacaaag
    33601 gtgccgatgt tcatccacgc cgccgccttt tggggctgcg gtctgctgcc gggctacctg
    33661 ctcgcctacc gtttcgatat gggcatttac ggcttctgga cggcattgat tgcctcgctc
    33721 accatcgccg ccatcgcctt ggtgtggtgc ttggaattgt gcagtaggga gatggtcaga
    33781 tcgcataagg ccgtctgaaa actccgccga ttaaaaatat agtggattaa caaaaaccag
    33841 tacggcgtta cctcgccttg ccgtactatt tgtactgtct gcggcttcgt tgccttgtcc
    33901 tgatttttgt taatccacta tagaaacgat gggctgaagc ccgccactgg caatactcat
    33961 tttaaagttc aaacccatgc aacccatccg ataccgaacc gaccttaccc cctacaacac
    34021 tttcggcctg cgcgcccaag cccgagcctt tattgcgctt gaacacgccg acgggttgcg
    34081 cgacatcgtc cggctacccg agttcaaccg agatactgtt ttatggctgg gcggcggcag
    34141 caatatcctt ttgatggagg actacgccgg tctggtcgta catatggaaa acaaaggcat
    34201 acgcgaaatt gctcgttcag acggcatggt tctgattgaa gcgcaggcgg gcgaaatctg
    34261 gcacgatttt gtcctgcacg ccgtcgcgct gggtttgaac ggtttggaaa acctgagcct
    34321 gattccgggt acggtcggcg cgtcgcccgt gcagaacatc ggcgcatacg gcgtagaggc
    34381 gaaagatgtg attcacagcg tgcgctgctt cgatttggat acggaaacct ttgtaaccct
    34441 ttccaatgcc gactgccgct tcgcctaccg cgaaagcctg ttcaagcagg aaggtaaagg
    34501 gcgttatgtg attgtttcgg tcgtatttgc attaaaaacg cattttgtgc cgaacttggg
    34561 ttacggcgat ttggcggccg ccgttgccga actgagcgcg ggcagggtgc cgacggcgaa
    34621 agatgtttcc gatgcagtgt gtgcaatccg caacagtaaa cttcctaatc ctaacgtgct
    34681 tggcaatgtc ggcagtttct ttaaaaaccc cgtcgtcagc gcagaaaaag ccgccacctt
    34741 gttgcagcgg catcctgata tgccgcgcta tccgcagccc gacggttcgg tcaaactcgc
    34801 cgccggctgg ctgatcgacc aatgtcgtct gaaaggcttt caaatcggcg gcgcggcggt
    34861 acatgacagg caggctttgg tcttagtgaa caaaaacaat gcctcggcaa acgatgtccg
    34921 gcaattggcg caacacatca aatttacagt atttgctcgg tttcaggtag aattgcacgc
    34981 cgaacccaat tggctgcctg cttcgttcag cctgtaaatc caaggagtat gcccgtgacc
    35041 cgccccgcca aaatcaatac ttacacacgc atcatcgacg ccagccttgc gcttttcaac
    35101 gaggaaggcg agcgcaacat cagcaccaac catattgccg cccacttggg catcagtccg
    35161 ggcaacctct attaccactt ccgcaacaaa gacgaaatca tcgtccaact gttcaaacgt
    35221 tacagcgaag ccctgctgga atacctcaac gaagccgtgt tgccgtctga tgtggaagac
    35281 tccatcaatt atatggcggg catttacgac gtgatgtggg aataccgctt cctattcagc
    35341 gacgtgaaca ccctgcttgc acgcagtgcc gaactattgg gcgaacacaa taccttcacc
    35401 caagccaaag tctccccgct cttggtcaac ctgctgaccc agctcaacgg tctgaacatc
    35461 atccaagccg accaaaccgc catgaacgac ctcgccgtca atatgtggat gatcacgaaa
    35521 tactggttcg acttcgacag ctccctgcgc ggtcgtacca agctgaccga agactccaaa
    35581 gcacgcggca tccgccgcac cttaagcctc cttcgtcctt atcttttgcc cgaacaccgc
    35641 aagaaatacg accggaaaat cggcaacggc aacccgtaaa cccacagcgt ccgaatcagc
    35701 cttcagacgg cacttacccg acggtatcag cgaatgcgcg cagtcccccc gccccatatc
    35761 tccatcacag gcctaggtta cctcggcctg ccgctggcac aaaagtttta ccaacacggc
    35821 agccgcgtcg ccgccataaa acgcagcctg acttcggacg acatcaacct gcccatacac
    35881 ctcgatacca tcgacctcaa tcaagacagc gcgtttcaaa gcgcgaacct tgcccgagat
    35941 acaagctttt ggcggcacca tgccaacaaa cccgtttggt tctgcctctt gccgccatca
    36001 tcgctgacac attacgccga taccgtcaaa caatgggcag aacttgcccg ggcgtgcaac
    36061 gtgcaacacc tgattttcac aagcagtacc agcgtttacg gcgatacagc gcgcgaatgc
    36121 gacgaaatcg ccctacccga tccgcaaacc gagtccgccc gccaaatcct cgccgccgaa
    36181 caacacctgc tcgacagcgg catctccaac atcgacatcc tgcggctggg cgggctttat
    36241 tgcgccgaac gccatcccgt cagccgcctt gttcaaaagc aaaacatccc gggcggcaac
    36301 cgccccatca acatcgtcca ccgtaatatc gccgtcgaaa gcctgtttca gacggcattt
    36361 aaccccggcg gcaggcggct gaaaaacatt atcgaaccgc gccacccgac acgacgcgaa
    36421 ttctatacgg aagaagccgc caaactcggc ctgcccgcgc cggatttcgc acccgacgac
    36481 agcgtgggca aaatcatccg taccgtttgc gataacggct taagcctgta aaataagcgt
    36541 caacagcaac aaacaggcat tctccgcccc gatcagaaaa tatgtccgcc ctcctcccca
    36601 tcatcaaccg cctgattctg caaagcccgg acagccgctc ggaacttgcc gcctttgcag
    36661 gcaaaacact gaccctgaac attgccgggc tgaaactggc gggacgcatc acggaagacg
    36721 gtttgctctc ggcgggaaac ggctttgcag acaccgaaat taccttccgc aacagcgcgg
    36781 tacagaaaat cctccaaggc ggcgaacccg gggcgggcga catcgggctc gaaggcgacc
    36841 tcatcctcgg catcgcggta ctgtccctgc tcggcagcct gcgttcccgc gcatcggacg
    36901 aattggcacg gattttcggc acgcaggcag acatcggcag ccgtgccgcc gacatcggac
    36961 acggcatcaa acaaatcggc aggaacatcg ccgaacaaat cggcagattt tcccgcgaac
    37021 ccgagtccgc aaacatcggc aacgaagccc ttgccgactg cctcgacgaa ataagcagac
    37081 tgcgcgacgg cgtggaacgc ctcaacgaac gcctcgaccg gctcgaacgc gacatttgga
    37141 tagactaacc ttcagacggc atccgacatg aacagccctt ttcacaacat cggcatcgta
    37201 acccgcccca acacgcccga catccaagac accgcacaca cgctgattac ctttttgaag
    37261 cagcacggct ttaccgtcta tctcgacgaa gtcggcataa aggaaggctg catctatacc
    37321 caagacaccg tcggctgcca tatcgtcaac aagaccgaac tggggcaata ctgcgacctg
    37381 gtcgccgttt taggcggaga cggcaccttt ctctccgtcg cccgcgaaat cgccctgcgc
    37441 gccgttccga ttatcggcat caaccaaggg catttgggct tcctgaccca aattccccgc
    37501 gaatatatga cggacaagct attgcccgtt ttagaaggga aataccttgc cgaagagcgc
    37561 atcctgattg aagccgcact gatccgaaaa ggcaaaaccg ccgaacgcgc catcgccctc
    37621 aacgatgccg tcctctcccg tggcggcgcc ggacagatga ttgagtttga agtcttcgtc
    37681 aatcgggaat tcgtctatac ccagcgttcg gacgggctga ttgtctccac ccccaccgga
    37741 tcgaccgcct attcgcttgc cgccggcggc cccatcatgc aggcaggttt gcacgccttc
    37801 acgctcgtcc ccatctgccc gcaatccatg accaaccgcc ccatcgccat tccagacacg
    37861 tccgaaatcg aaatcctcgt tacccaaggc ggcgacgcgc gcgtccattt cgacggtcaa
    37921 acccatatcg acgtgcaaaa cctcgaccgc attaccatcc gccgctaccg aaacccctta
    37981 cgcatcctac atccgaccga ctaccaatat ttcaaaaccc tgcgccaaaa actgcactgg
    38041 ggcgagcaat tagtctaagc cggcctttac ccgaaagtat ccatgaattc caaaatttcc
    38101 tccgaccata cagaagatgc cgtgcgcctc tccaaacgca tggcgcaact ggggctttgt
    38161 tcacgccgcg aagccgacgg ctacatcgga cagggctggg taacggtcaa cggcaaaacc
    38221 gccgtactcg gtcagaaagt ttcaccggca gaccgtatcg aactgaacaa gaaagcccac
    38281 gaacagcagg cggcacgcat taccatcctg ctgaacaaac ccgtcggcta tgtcagcgca
    38341 caggcggaaa aaggctataa atccgccgcc gaactgatta cctctgaaaa tcgctgggaa
    38401 ggtgacacca gccgcatccg tttcgatccg aaacacaaaa tcggtcttgc ccccgccggc
    38461 aggctcgata tcgattcagt cggattactg gtgttgacgc aggacggccg tatcgccaag
    38521 cagcttatcg gcgaaaacag cggcagtgaa aaagaatatt tggtgcgcgt gcgcggcaaa
    38581 ttggacgaaa aaggacttgc cttactgaat cacggattga gtttggacgg cgagaaactg
    38641 cgtcccgccc aagtagaatg gcaaaacgaa gaccaactgc gcttcgtgtt gaaacagggt
    38701 aaaaagcggc aaatccgccg tatgtgcgaa ctggtcggac tgcgcgtcgt cgggctgaaa
    38761 cgcatccgca tgggcaaggt caaactcggc aggctgccgc ccggcaaatg gcgttatctc
    38821 gcccccggtg aatcgtttta aataagcatt aaaaaatgcc gtctgaaaat ccttgcgttt
    38881 tcagacggca ttgccttatg cccaaataac ggtttcttcc aagggcctgc gcgccttcgt
    38941 ggcgatttcc tgaacgcggt aaccgaatgt cgcggcgacg gacacgcccc attctgccgc
    39001 atcgaacaaa ccgaactgcc cggacaatac gcgttccata tcggcatagt tgaaaccttc
    39061 caccgggcag gaatcgatac ctgccatcgc cgcacccgtc atcatgttgc ccaacgcgat
    39121 ataggtctgg cggcaacacc agtcaaacaa ggcgcgcgaa tcgtccaaaa tcttgatgtc
    39181 gtcggcttga aacgcctgat acctcgccaa agattttgcc atggcatccg gttcggtaac
    39241 gccgcgccgt ttgaggcttt ccaacataaa cgggctgtcg aagcgggcat ttttctttgc
    39301 caaaaacacc accaaatgac tggcggtatc caaagcatcc gccatacccc aagaaaacgg
    39361 cttgattgcc tgtcggattt cggggttttg aaccacaaca aactgccaag gctccgaacc
    39421 gaccgaactg ggcgacaaac gcccgagttc taaaataaac tgaaaatcct cggcactgat
    39481 tttgcgtgcc gcatcgtaat gccggcatga tttgcggttt ttaaatgcgg atagaacctg
    39541 ctctctgtct aataccgtca tagtgcatcc tttcattact tgtattgcaa attgatttca
    39601 aaccgatacg gcggcagatt cgccaataac gcccaaacgc ctttcagacg gcatttggtt
    39661 catcgcaaac acgcctacgg gtaggcgata taggcataag ccgaatggtt gtggatagac
    39721 tcgaaattct cgctctcgac gacgaaactc ttgatgcgtt tgtcggcgat cagcgaagta
    39781 gcgacatcgc gcaccatatc ttccacgaat ttcgggtttt cgtaggcctt ttcggtaacg
    39841 tatttttcat cggggcgttt gagcaggccg tagagttggc agctcgcctg cgcctccaca
    39901 taatcgatga cttcctcgat accgacttcg gcatcggcag tcaggctgac ggtaacatgc
    39961 gaacgctgat tatgcgcgcc atattgggaa atttctttgg aacacgggca aagcgaggtt
    40021 acgggaatca tgaccttcat actgtggccg tatgccccgt ctttgatttc gcccgtgaga
    40081 cagacatcat aatccagtaa ggaccgaata ccggaaaccg gcgcggtttt cttgcggaaa
    40141 aacggaaaag aaacgctgat tttgccggcg cgggaatcta aaagcgcgac catttcggta
    40201 gtcagcttgc gcaattgtgc aaaatccaag gcttcggcat gttgctccat caatgcgaca
    40261 aaacgcgaca tatgcgtccc cttctgctca gcaggcaggt aaaccgtcat ggtcagacgg
    40321 gcaatggtgg actggatgcc ttctgcagtt tgcagggtaa tcggaaagcg caggtctttg
    40381 ataccgacct gattgatcgg cagattgcgc aaatctcggc tggattgcac gtctgcaata
    40441 gtgttcatga gttgtttttc cttaatattc atgtaattgt tgagatgtgt gcatggtatg
    40501 gcgtttccgc cggggaatat accgtcaatc tgcaaatgcg agattatatc actcgctttt
    40561 agcagctgca ttgattgttt ctatttttca gatagtgata aaatcttcga ttattcacat
    40621 ctacataggc agagaccaaa tgaaattcgc cacgaaagcc attcattcca gctacgattg
    40681 cgacgaacac aaccgcgcgc tgatgccgcc gatttatcaa aacagtatgt ttgcgttgca
    40741 cgagattggc gaaaatgtgc cttaccgtta ttcgcgcctg agcaacccga cccgtcagat
    40801 tttagaagac accgttgccg atttggaaca cggtgcggca ggttttgcgt tttccagcgg
    40861 tatggcggga attgatgccg tatggcgcac tttcctgcgc ccgggcgata ccattgtcgc
    40921 cgtcgccgat atttacggcg gcgcttatga tttattggtc gatgtttatc aaaaatgggg
    40981 ggtgaacgtt gtttttgccg atttaggcaa tccggataat ttggacgaac tgcttaaagc
    41041 gcacaaggtc aaactggttt ggctggaaac gccgtccaat ccacttttac gcttggtaga
    41101 catcaaagtc cttgccgcaa aagccaaagc agccggtgcg ctggtcggta tcgacaacac
    41161 ttttgccacg ccgtatctgc aacagccgtt ggatatgggt tgcgattttg tattccattc
    41221 cgctaccaaa tatttgtgcg gccattccga cgtgttgatg ggcatcgtcg ttgccaaaac
    41281 caaagaactg gcgcagcctt tgcacgatat gatggtgcat accggcgcgg ttgccggccc
    41341 gctggactgc tggctggtgt tgcgcggcat caaaacactg gctctgcgca tgaacgccca
    41401 ttgccaaaac gcactcgaaa tcgcgcgccg tttggaagcc catcctgcca ttgaaaaagt
    41461 gttccatccc ggcctgccgt ctcacgaaca ttacgaactg gcgaaaacac aaatgcccaa
    41521 aggcatcggc ggcgtggtta cggtttatct caaaaacgac acgcgtgaag cggcaaacag
    41581 cgtgattaaa aacatgaaac tggtcaaaat ggcttccagc ctcggcggtg tggaaagttt
    41641 ggtcaaccat tgctattccc agtcccacag cggcgtgccg cataatgtga aaatggaaat
    41701 gggcatcaaa gtcggtctgc tgcgtttctc catcggcatc gaagacgcgg acgatatttg
    41761 gaacgatatt tccgccgcac tcgatacaac tttgtaaact gtaaaaatgc cgtctgaaac
    41821 catggtttca gacggcattt caattaaccc ggccgaaaat caacgcttca acatctttgc
    41881 cgcctcaata gcgtaatagg tcaaaatccc gtccgcaccc gcacgtttga atgccagcag
    41941 gctttccaaa accactttgc cgccgtccag ccagccgttg gcaatcgctg cctgcaacat
    42001 cgcgtattct cccgaaacct gataggcata agtcggcaca ccgaactcgt cctttacgcg
    42061 gcggacaacg tccaaatacg gcaaaccggg cttgaccatt accatatccg caccttcctg
    42121 aatgtccaac gccacttcgt gcaacgcctc atcggtattt gccggatcca tctggtaggt
    42181 ctttttatct gccttgccca aattgcccga actgcctacc gcatcacgga aagggccgta
    42241 aaatgcagaa gcatatttgg cggaatacgc cataatccgc gtatggatat gcccggcatc
    42301 ctccaacgcc tcgcgaatcg caccgatacg cccgtccatc atatcggaag gggcaaccac
    42361 ctgcgcgccc gcttcagcgt ggcacaaagc ctgcttgacc aaaacctcta cggtttcatc
    42421 gttcatcaca taaccgtttt cgtccgtcag cccgtcctga ccgtgaaccg tataaggatc
    42481 gagcgcgaca tccgtcataa tgcccagttc gggaaacctc tcgcgcaggg cgcggacggc
    42541 tgccggcacg agtccttcgg gattgtacgc ttcctctgca cgctcggttt tgtttgccgt
    42601 aaccacggga aagagtgcca acatcggaat accgagcttt accgcctctt ccgccgtaaa
    42661 cagcagcctg tccaaacttt gacgcttcac acccggcata gaaggcacat cctcctcgcg
    42721 cgccgacccc tccaatacga acaccggata aatcaaatca tcggcggtca gcgtgtgttc
    42781 gcgcatcagg cggcgtgaaa aatcgtccct gcgcatacgg cgcatacgcg aagccggaac
    42841 attgcggtaa ggaaactgca taagccctcc aatcatcctg tcaacaattc aaaccatacg
    42901 gaagccgccg caacttgcag acagcatcgg gcggcaatca aaatccgttt tcaccggaaa
    42961 gggtttcggc ccgccgaaat gccgcgccga aggacggcgt cccgcccatt aaagaacaat
    43021 agcaaaacaa cagcttcagg ctgtcagcct tcgatattct tacgccacaa taccaaaagt
    43081 ttcccgatat gctgaacagg ttgcgcgtca accgcctcac ataaggcagt gcagatttcg
    43141 atacgttcgg cacggtcgtc gccgaatacg cgcactttaa tcagctcatg cgccgtcaat
    43201 gccgcatcgg tttccttgat gaccgcgtcc gtcagaccct gctgaccgac catcacaaca
    43261 ggatggagat ggtgcgcgcg cgctttcagt tccaaaattt ctttggtgtt caatttggta
    43321 tccgtcattt tcctactgct tgaataaaaa gaataatgcg gcattgtacg cgatttggca
    43381 cgacatcggc aaaattaacg tacaatacgc ggtttccatt ccgacccgcg aaccactatg
    43441 gctgtacgtt ccaaatcctc aaaagcgtgg ctgcacgaac acgtcaacga ccactacgtc
    43501 catatggcgc aaaaagacgg ctaccgcgcc cgtgccgcat acaaactttt ggaaatcaac
    43561 gacaaagaca aattaatcaa acccggcacg gtacttgccg acttgggcag cgcgccggga
    43621 agctggtcgc aggttgccgc caagctgacg ggttcttccg gagcagtttt cgccttggac
    43681 atcctgccta tggaagccat agggggcgtc tccttcattc agggcgactt ccgcgagaac
    43741 gacgtactgg cacaattcga aaccttgttg gacaaccgcc cgctcgacct tgtaatttgc
    43801 gatatggcgc ccaatatgtc gggaaacgcc gtaagcgatc aggtacgcag cttttatctg
    43861 tgcgaactgg ctttagactt cgcctcgcaa cacctgaaaa ccggcggcag ctttttggtc
    43921 aaagtctttc agggtgcagg ctatcaggaa tacatggcag ccatgcgcga aattttcggc
    43981 acggtgcaga cgcgcaaacc cgaagcctcg cgcaatcgct ccagtgagat ttatttattg
    44041 ggcaaaaata aacgctgaca atacagacgg cgtgctttac aatcatttcc gttttacacc
    44101 tcaattatgg agccttgcta agtggggaac acctttaaat caatccttgt ctgggtcgcc
    44161 ttgggtatcg gcctgatggc tgcgttcaac gctttagacg gtaaaaaaga agacaacggg
    44221 caaatcgaat attctcagtt catccaacag gtcaacaacg gcgaagtatc cggcgtcaac
    44281 atcgaaggat ccgtcgtcag cggctacctg attaagggcg agcgcaccga caaaagcacc
    44341 ttcttcacca acgcgccttt ggacgacagc ctgattaaaa cactgctcga caaaaacgtc
    44401 cgtgtaaaag taacgccgga agaaaaaccg agcgcgctgg ctgccctgtt ttacagcctg
    44461 ctgcccgtcc tgctgctgat tggcgcgtgg ttctacttta tgcgtatgca gacgggcggc
    44521 ggcggcaaag gcggcgcatt ctcattcggc aaaagccgcg cccgcctact ggacaaagat
    44581 gccaacaaag tgacctttgc cgatgtcgcc ggctgcgacg aagccaaaga agaagtgcag
    44641 gaaatcgtcg attacctcaa agcgccgaac cgctatcaaa gcctcggcgg tcgcgtgccg
    44701 cgcggcatcc tgctggcggg cagcccgggt acgggtaaga cgcttttggc aaaagccatt
    44761 gcaggcgaag ccggcgtgcc gttcttcagc atttcaggtt ccgactttgt cgaaatgttc
    44821 gtcggtgtcg gtgcaagccg cgtccgcgat atgttcgagc aggcgaagaa aaacgccccc
    44881 tgcatcatct ttatcgacga gattgacgca gtcggccgcc aacgcggcgc aggtttgggc
    44941 ggcggcaatg atgagcgcga gcaaacatta aaccaattgt tggttgaaat ggacggtttt
    45001 gagagcaatc agactgtaat tgtgattgcg gcaaccaacc gccccgacgt actcgatcct
    45061 gcgctgcaac gccccggccg tttcgaccgc caagtggttg tccccctgcc ggacatccgg
    45121 gggcgcgaac agattttgaa cgtccactct aaaaaagtgc ctttggacaa atctgtggat
    45181 ttattgtccc tcgcgcgcgg cacgccgggt ttttccggcg cggatttggc gaacttggtc
    45241 aacgaagccg ccctgtttgc cggccgccgc aataaagtca aagtcgatca gagcgatttt
    45301 gaagacgcca aagacaaaat ctatatgggt ccggaacgcc gcagtatggt gatgcacgaa
    45361 gacgaaaaac gtgcgacggc gtatcacgaa tccggacacg cgattgttgc cgaaagcctg
    45421 ccctttaccg accccgtcca caaagtaacc attatgccgc gcggacgtgc gctgggtctg
    45481 acttggcagc ttccggagcg cgaccgcatc agtatgtata aagatcagat gttgagccag
    45541 ctctccatcc tgttcggcgg acggattgcc gaagacatct tcgtcggacg catctccacc
    45601 ggcgcatcaa acgactttga acgcgcaacc caaatggcgc gcgaaatggt aacgcgctac
    45661 ggcatgagcg acaaaatggg cgtgatggtt tatgcggaaa acgaaggcga agtcttcttg
    45721 ggacgcagcg taacccgttc gcaaaacatt tccgagaaaa cccagcagga catcgatgcg
    45781 gaaatccgcc ggattttgga cgagcagtat caggttgcct acaaaatcct cgatgaaaac
    45841 cgcgacaaga tggaaacgat gtgcaaagcc ctgatggaat gggaaaccat cgaccgcgat
    45901 caggtactgg aaatcatggc gggcaaacaa cccagcccgc ccaaagatta cagccacaac
    45961 ctgcgcgaga atgcggacgc ggcggaagat aacgcgccgc acgctccgac tcgggaagaa
    46021 accgaagcac ctgccccggc agacaccgct tcgacagagt ccgagcagca gcctgaaaac
    46081 aaggcttaac ttcccgaaca aacggcagcc cgcaagctgc cgttttccca tcctgttttc
    46141 agacggcatt ttccgctcaa tgccgtctga aacgccaaag ccgtatcggt ttttgcgaat
    46201 ctgcgatatg gcggtttaaa ttgaaatcag aacggggcaa ggctgtaccg gtttaaaatt
    46261 aaaccgctat aataccgccc ttcgaataca cgcccgcatt ggtgatgccc gtgaaaaaag
    46321 ttgaaaaaaa catcttggtg ctgcacggcg cggacaaaat gttcgagctg gtcgataagg
    46381 ttgaggatta cccgaacttt ctgccgtggt acagcaaaac cgaggtcatc ggacgcaacg
    46441 gcaacgaact gaaggcgcgg ctgtttatgg actatatgca cgttcgccaa tcattcgcca
    46501 cgcacaaccg caacattccg ggcagggaaa tccgtatgga actgctcgaa ggtccgttca
    46561 aaaccttacg tggaacgtgg aaatttatcg atttgggcga cgatatgtgc aaaatcgaat
    46621 tcaatttgga atacgatttt tccaatgccg ttttgtccgc cttaatttcc cccgtcttca
    46681 accacctttc cgccacgctg gtcgaagcgt tcgtcaaaga ggcagaccgc cgttatgctt
    46741 gaaattgaga ttgtgtacgg gctgcccgac cgacaggttt tgaaaaccat gcagcttgcc
    46801 gagggaacaa ccgtccgcgc cgccgcactg caaagcggtt tggacggcat atttgaagat
    46861 ttaaacctgc attccgcgcc tttgggcatt ttcggcaaag ccgtcaaaga cgacacgccc
    46921 ctgcgcgacg gcgaccgcat cgaagtgtac cgcccgctgt tgatcgaccc caaagaagcg
    46981 cgccgcaaac gcgttcaaaa tcaagaagaa taaccatgcc gtctgaagcc ttcagacagc
    47041 attcgacaga aacacggaaa atatcatgtc aaacaaaatc aaaatgcttg tcggattggg
    47101 caaccccggc aaagaatacg aacaaacccg acacaatgcg ggtttttggt tcctcgacga
    47161 actggcgtgg aaatggaagg cttcatttaa agaagaaaaa aaattcttcg gcgaagtcgc
    47221 ccgtgccgcc ctgcccgacg gcgacgtttg gctgctcaaa cctgccacgt tcatgaaccg
    47281 ttccggacag gcagttgccg cgcttgcaca gttctacaaa atcaaacccg aagaaatcct
    47341 cgtcgtccac gacgaactcg acattccctg cggacggatt aaattcaaac tcggcggcgg
    47401 caacggcgga cacaacggct tgaaagacat tcaggcaaaa ctcggcacgg cagactatta
    47461 ccgcctgcgc ctcggtatcg accacccggg cgaccgcaac ctcgtcgtcg gctatgtcct
    47521 gaacaaaccc agtacggaac accgccgaca gattgacgat gccgtcgcca aatccctgca
    47581 agccataccc gacatccttg ccggcaaatg ggaagaagca acccgcttcc tgcacagcaa
    47641 atgacccgat gccgtctgaa gccctttcag acggcattcc cgccatcagc gagaacccgc
    47701 actatgtccg aactcaaccg gctcatccaa accgaatccg ttgccgtcat tgaagaaact
    47761 ttaaattttc tgctctacga atgcagcata gacgatgccc cctccgccga agaaattgcc
    47821 gtttggcgcg atatgctggc cgcacgcggc ggaaaattcc tgcgcctatc caaactatgc
    47881 cagacatggc ttgaagagga acaagcatga atctgccacg caaccgcttt atcctgctct
    47941 cggcattgtg gtttgcaggc agcatttact cactgctttt caaagctgcc gaaaccgcgc
    48001 caccgccttt tccgcatttt gacaaagtgg cgcacctcgc cctgtttttc gcacaaatct
    48061 ggcttctgac caaagcattc agaaccgaca accgccccat cccctatcgc agcctgatgg
    48121 tctttgccct ctgtttcgcc ctcttcagcg aatgcgcgca ggcatggttt accgcaacga
    48181 gaaccggcag tttgggcgat gtccttgccg acctgacggg cgcagccctt gccctcttta
    48241 ccgcgcgagc tgcctgccgc cctgactaaa tcggtttatt ttccccaaac aggatgcacc
    48301 tcttcccgat atatcctatt tttcccctta catatcaatc ccatctcata aacaaaaccg
    48361 acaaatcgtt tacaatatat ttacactaca tcagattaca aatatactcg aaccagttgc
    48421 agaagcggcg attccacaaa ccgtttcgga ttgcacgaca caaaagaaaa accgattttg
    48481 ttgccttaaa ggagtattca tgaacctgca tgcaaaggac aaaacccagc atcccgaaaa
    48541 cgtcgagctg ctcagtgcgc agaagccgat taccgacttt aagggcctgc tgaccaccat
    48601 tatttccgcc gtcgtctgtt tcggcattta ccacatcctg ccttacagcc ccgatgccaa
    48661 taaaggtatc gcgctgctga ttttcgttgc cgcactttgg tttaccgagg ccgtccacat
    48721 taccgtaacc gcactgatgg tgccgattct cgccgtcgta ctcggtttcc ccgacatgga
    48781 catcaaaaag gcgatggctg atttttccaa cccgattatc tacatttttt tcggcggctt
    48841 cgcgcttgcc accgccctgc atatgcagcg gctggaccgt aaaatcgccg tcagcctgtt
    48901 gcgcctgtcg cgcggcaata tgaaagtggc ggttttgatg ttgttcctcg ttaccgcctt
    48961 tctgtccatg tggatcagca acaccgccac cgccgcgatg atgctgcctc tagcaatggg
    49021 tatgctgagc cacctcgacc aggaaaaaga acacaaaacc tacgtcttcc tcctgctcgg
    49081 catcgcctat tgcgccagca tcggcggctt gggcacgctc gtcggctcgc cgcccaacct
    49141 gattgccgcc aaagccctaa atctggactt cgtcggctgg atgaagctcg gcctgccgat
    49201 gatgctgttg attctgccct tgatgctgct ctccctgtac gtcatcctca aacctaattt
    49261 gaacgaacgc gtggaaatca aagccgaatc catcccttgg acgctgcacc gcgtgatcgc
    49321 gctgttgatt ttccttgcca cagccgccgc gtggatattc agctccaaaa tcaaaaccgc
    49381 cttcggcatt tccaatcccg acaccgttat cgccctgagt gccgccgtcg ccgtcgtcgt
    49441 cttcggcgtg gcgcaatgga aggaagtcgc ccgcaatacc gactggggcg tgttgatgct
    49501 cttcggcggc ggcatcagcc tgagcacgct gttgaaaaca tccggcgcgt ccgaagcctt
    49561 gggacagcag gttgccgcca ccttttccgg cgcgcccgca tttttggtga tactcatcgt
    49621 cgccgccttc attatttttc tgaccgagtt caccagcaac accgcctccg ccgcattgct
    49681 tgtaccgatt ttctccggca tcgctatgca gatggggctg cccgaacaag tcttggtatt
    49741 cgtcatcggc atcggcgcat cttgtgcctt catgctgccg gttgccacac cgcctaacgc
    49801 gattgtgttc ggcacgggct taatcaagca acgcgaaatg atgaatgtcg gcatactgct
    49861 gaacatcctc tgcgtagtat tggttgctct gtgggcttat gctgtactga tgtaaaccat
    49921 cgacctaaac aacaagaccg tctgaaagaa tatttttcag acgaccttga agtttgtcgt
    49981 tcagacacaa tttgtcgaat cattcaaaac cagattctaa cgaaaggaaa cccatgatta
    50041 tcctgcacac caacaaaggc gacatcaaaa tcgaactcga tttcgacaaa gcccctgtta
    50101 ccgccaaaaa cttcgagcaa tacgtcaaag acggcttcta cgacggcgta atcttccacc
    50161 gcgtcatcaa aggcttcatg attcaaggcg gcggcatgga tgaaaacatg aacgaaaaag
    50221 aaacccgcga tccgattcaa aacgaagcgt ccaacggcct gcccaacgat aaatacacca
    50281 tcgccatggc acgcacttcc gacccccatt ccgccagcgc gcaattcttc atcaacactg
    50341 ccgacaacgc tttcctgaac ttccgttcta aagagctgta cggcaaaacc gtcgtccaag
    50401 actggggcta tgccgtattc ggtaaagtcg ttgacggttt tgacgttgtc gatgccatcg
    50461 aaggcgtttc taccaaacgt catggttacc acgacgacgt accgagcgaa cctgtcgtca
    50521 tcattaaagc cgaagcggta taaaccgaca atccgaaagc agcctgcaca aaggctgctt
    50581 ttatttcaga cggcatgtta tttgatgtaa ccgtgctatg ccgtctgaaa accggacgca
    50641 agccttcaga cggcataccg ctatgttaaa atatgcccgt ttttcctaaa cggacacaaa
    50701 ggaaacctta tggcaagcat cgcccgcgac atcttcaaag cctacgacat ccggggtatt
    50761 gtcggcaaaa ccctgaccga cgaagccgcc taccttatcg gcaaagccat cgccgccaaa
    50821 gccgccgaaa aaggcattac ccgcatcgcg ctcggacgcg acggacgctt gagcggtccc
    50881 gaactgatgg aacacatccg gcgcggcttt accgacagcg gcatcaatgt cctcaatgtc
    50941 ggtatggttg ccactcctat gctctacttc gccgccgtca acgaatgcgg cggcagcggc
    51001 gtgatgatta ccggcagcca caatccgccc gattacaacg gctttaaaat gatgctcggc
    51061 ggcgacacgc ttgccggcga agccatccaa gaacttttgt ccatcattga aaaagacggt
    51121 tttgctgccg ccggcaaaca aggcagcgtc accgaaaaag acatctccgg cgaatacctc
    51181 aaacacatta ccggacacat caggctcaaa cgcccgatga acatcgccat tgacgcgggc
    51241 aacggcgtgg gcggcgcgtt tgccggcaaa ctctacaaag gcttgggcaa cgaagtaacc
    51301 gagcttttct gcgacgtgga cggcactttc cccaaccacc atcccgaccc atccaaaccg
    51361 aaaaacctgc aagatttgat tgccgcgctg aaaaacggtg atgccgaaat cggcttggcg
    51421 tttgacggcg atgccgaccg cttgggtgtg gttaccaaag acggcaacat tatttatccc
    51481 gaccgccaac tgatgctgtt cgcccaagac gttttgaacc gcaatcccgg cgcgaaagtc
    51541 attttcgacg tgaagtccac ccgcctgctt gcgccttgga ttaaagaaca cggcggcaaa
    51601 gccataatgg aaaaaaccgg ccacagcttt atcaaatccg ccatgaaaga aaccggcgcg
    51661 ccggttgccg gcgaaatgag cggacacatc ttcttcaaag aacgctggtt cggcttcgac
    51721 gacggtctgt acgccggcgc acgcctcttg gaaatcctgt ctgcctccga taatccgtcc
    51781 gaagtgttaa acaacctgcc gcaaagcatt tccacgcccg aactcaacat cgccctgccc
    51841 gaaggcagca acggccatca ggttatcgac gaactcgccg ccaaagccga atttgaaggc
    51901 gcaaccgaaa tcatcaccat cgacggtctg cgcgttgaat ttcccgacgg cttcggtctg
    51961 atgcgtgctt ccaataccac gccgattctg gtgttgcgtt ttgaggcgga tacgcaagaa
    52021 gccatcgagc ggattcaaaa ccaattcaaa gccgtcatcg aaagcaatcc gaatctaatc
    52081 tggcctctgt aaacacagga aaaatgctta atcaaatctt ggtagattgg atttggctta
    52141 aaaatctccg cgtccgttcg tgtttggggt ggtcgaacaa atcttgcggg ctgccttgtt
    52201 cgacaataac gccgccgtcc atcacgacaa cggtcgtggc aacttccagc gcgaacttga
    52261 tttcatgcgt aacgacaacc atggtccagc cttcttgcgc caattccttc atggtatcca
    52321 aaacatcttg caccaattca ggatcgagcg cggaagtcgg ttcgtcaaac agcatcagtt
    52381 caggctgaat cgccaatgcg cgggcaatgc cgacgcgctg ctgctgaccg ccggaaagct
    52441 ggtagggata caaatccact ttgtcgccca agccgacttt ttccagcagt ttcagagcct
    52501 cttcgcgcgc ttgggcggca ggcttgccct gtacggcaac cggtccttcc attacgtttt
    52561 ccaaggcggt tttgtgcgga aagaggttgt attgttgaaa caccatgcct gatttgcggc
    52621 gcagtgccaa aatatcgtgt ttgcttggtt ttttagaaaa atcgattttc agcggtcgct
    52681 cgttgtcgaa ctcgatttgt ccgtcttcgg gcatttccaa cgcgtttagg catcgcagaa
    52741 acgtcgtttt gcctgagccg gaaggcccga ggataacgac cacctgccct ttgcccacat
    52801 ccaaatcgat gccgcgcaaa atagtgtttt cgccgaaggt cttatggata ttgcggattt
    52861 taatcataac aactccttat ttggcgacat aacggtcgaa acgtttttcc aaacgtgcct
    52921 gaatcaggaa cagaaccttg cagaaacacc agtaaaccaa tgcggcttca atatagacgg
    52981 gcaaaaagtc gtaagtgcgg tttgccgttt cctgcgcgac gcggaacaat tcagttaccg
    53041 ttaccaccgc tgcgagcgag gtgtttttga acaagccgat aaactcgttg ctcaaaggcg
    53101 gcacggcaac gcggaatgcc tgcggcgcga cgatgcggcg gaacgtctgc atataggtca
    53161 taccgatgga gaaacctgct tcccattggc ctttaggtac ggacaaaatt gccgcgcgta
    53221 tggtttcgga agcgtatgca ccgacattga gcgagaagcc gataatggcg gcgggaatcg
    53281 gatcgatata gataccgacg gacggcagcc cgtaaaacac aatcacaagc tgcaccaaca
    53341 gcggcgtacc gcgaatgacg gaaatataaa attccaccaa tttcagcagg attttccgcg
    53401 caatgccgcc ggcgggcata atccgcacca aagccacggc tacggcaata accataccga
    53461 taacgaaaga agctgccgcc aaaggcagag acaccgcgaa gccggctttg accataggca
    53521 aaaacgcgct gacaatcata tcggcgcgtg tttccgtcat aaacggcagc gaagcaagga
    53581 aattattgaa cactgatgtc ttttccgaag aattgttcgc ccagtttttt cagcgtaccg
    53641 tcggctttca gctcgttgat tgccgtactg aatttcgcca cggcttcgtc attgcccttg
    53701 ttgacaatca ggccggaacc gactttttca tcggcaggtg cggaccaaac gattttcacg
    53761 cccgcattcg ggtttttctt cagatagtcc aaaaccgcca attcgtcgtt cagggttgca
    53821 tcggcacgtt tttgttcaat cagggtcagc gattgcgcca aaccgtccac tgccaccaaa
    53881 tctgcgcctg cagctttggc tttttcgccg tagttgctgg tcagggattg tgcggttttc
    53941 acgcctttga tgtcgtcgat agatttgatg ttgctgtcgt tacgggcaac caatacggca
    54001 ccgctccagc tgtaaggatc ggatttgtcg aatgtcgctt ggcgttcggg gctggtcaga
    54061 ccgacttggt ttgccaccac gtcgaaacgc cccgccttca aacccgccat catcgaatcc
    54121 cattgcgttt ctttaaactc gactttcacg cccagttttt ccgccacggc gcgggttact
    54181 tccacatcgt aaccggtcag tttgccgtcc ttgtcgtggt aggtaaacgg tgcgtaagtg
    54241 ccttccgtgc cgacggtaac cgtgccttta ttgttgatgc gctcgattaa agaaccggaa
    54301 actgccgatt gtgcaggcgc ggaagatgct ccgctgccgc cttccgaacc gccgcaggcc
    54361 gccaaaacca atgcggcaat accgccgagt acgaattttt tcaacatcat cctctcctgt
    54421 aaaaagtgtg caagtgcggc attctaagtg aaattgcggc aaacacaaag aatccttttt
    54481 ttatatggtt attccaaatc ggaatggaaa agatatgccg aagccgccgg aagcggaaat
    54541 aaagggggcg ggaaaggcgg tgggcggcac gaaaccgaaa agcggcatca ggtatgcaaa
    54601 cggcggattc acgcacgccg ttccggcaaa aatccgcctg ccgattcggt gctataatcc
    54661 atccgaaaca gatgacacca aaaaggaaca tctcatgaac acgccccaat ccgccatcat
    54721 tcccgaccac gcccaagccg gcatctttat cgaagccgac ttcgccgcca accgcctcaa
    54781 cgatattaaa gccgcctgcc gcgcttcgct cgacgcgtta actgccttga aagcccgctt
    54841 tccggacgat attttgggtc tgaccatcgc cttcggcagc aaagcctggg caacattcgg
    54901 acatacggac gaaggcagtg aaatcaaacc cttctccgaa atgggcaacg ggcttgcgcc
    54961 gtccacgcag cacgatatgt ccatccacat ccaatccttc cgccaaaacg ccgcctacgc
    55021 gcttgcccaa tccgttttgg gcgcgttcgg cgacagcata tgcgtcgcgt ccgaagaaca
    55081 cggtttgcgc ctgtatcagg atcgcgggct ggacggtttc gtcgacggca cggaaaaccc
    55141 gcagggcgat gaaaatgtcc gcgaagtcgc catcatcccc gaaggaaaac ccgatgcggg
    55201 cggcagctat gtcctgctgc aaaaatacct gcacgatttg aaaaaatggg atgccgtccc
    55261 cgtcgccgaa caggaggcct cggtcggacg cggcaaggaa accgacgacg aattcggccg
    55321 cgatgtccgc ctgcccgatt cgcacctcgg ccgcgtcaac ctgaaagaaa acggagtcgg
    55381 cctgaaaatc gtccgccgca gcctgccctt cggcaaaatc agcggcgaac acggtttgat
    55441 gtttaccgca tactgccgca cgctgcacaa tatcgaagcg caattattga gtatgttcgg
    55501 cgatacggac ggcaaaaccg acctgctgct ccgacacctc tccgccgccg tttcgggcgg
    55561 atactactac gcgccgtctg tcgaacgcct gcaaaacctc taagccggac gatgccgtct
    55621 gaaaccattt ttcagacggc atttt
//
LOCUS       DAXHSF010000002        38326 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.002, whole genome shotgun sequence.
ACCESSION   DAXHSF010000002 DAXHSF010000000
VERSION     DAXHSF010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 38326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38326
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.002"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(142..232)
                     /locus_tag="ACFM8S_000057"
     tRNA            complement(142..232)
                     /locus_tag="ACFM8S_000057"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(196..198),aa:Ser,seq:gga)
     gene            complement(268..354)
                     /locus_tag="ACFM8S_000058"
     tRNA            complement(268..354)
                     /locus_tag="ACFM8S_000058"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(318..320),aa:Leu,seq:cag)
     gene            443..2818
                     /gene="rnr"
                     /locus_tag="ACFM8S_000059"
     CDS             443..2818
                     /gene="rnr"
                     /locus_tag="ACFM8S_000059"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFC6313208.1"
                     /translation="MNKNIKSLNLREKDPFLSREKQRYEHPLPSREWIIELLERKGVP
                     SKIESLARELSITEDEYVFFERRLKAMARDGQVLINRRGAVCAADKLDLVKCRVEAHK
                     DGFGFAVPLTPAKDGDFVLYERQMRGIMHGDIVTVRPAGMDRRGRREGTVLDIVERAQ
                     SKVVGRFYMDRGVAILEPEDKRLNQSIVLEPDGVARFKPESGQVIVGEIEVYPEQNRP
                     AVAKIIEVLGDYADSGMEIEIAVRKHHLPHQFSEACAKAAKKIPDHVRKSDLKGRVDL
                     RDLPLVTIDGETARDFDDAVFAEKIGRNYRLVVAIADVSHYVRPDDAIDTDAQERSTS
                     VYFPRRVIPMLPENLSNGICSLNPNVERLCVVCDMVITYAGNIKEYRFYPAVMRSHAR
                     LTYNQVWKWLSGGIGHPFKTQIDTLYKLFKILQKKRFERGAVEFDSIETQMLFDDNGK
                     IEKIVPVVRNDAHKLIEECMLAANVCAADFLLKNKHTALFRNHLGPTPEKLATLREQL
                     GLLGLQLGGGDNPSPKDYAALVEQFKGRPDAELLQVMMLRSMQQAVYEPHCDGHFGLA
                     YEAYAHFTSPIRRYPDLTVHRAIKAVLNQQTYTPKKSWQALGVHTSFCERRADDASRD
                     VENWLKTYYMRDKVGEVFEGKISGMTSFGIFVTLDGIHIDGLVHISDLGEDYFNFRPE
                     IMAIEGERSGIRFNMGDRVAVRVARADLDDGKIDFVLIAGGSGRGRKVKSSASAKPAG
                     TAGKGKPKTAAEKKTARGGKVRGRGASAAAESRKKAKKPVPIKVKKRKGKS"
     gene            complement(2813..3010)
                     /locus_tag="ACFM8S_000060"
     CDS             complement(2813..3010)
                     /locus_tag="ACFM8S_000060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313209.1"
                     /translation="MPSERRLRPVCRIPLAFKTAVEINAIFQYGRFDWPEMMRRPPTS
                     KNRVEASGKYAPPPQAAPSAL"
     gene            complement(3157..4620)
                     /gene="guaB"
                     /locus_tag="ACFM8S_000061"
     CDS             complement(3157..4620)
                     /gene="guaB"
                     /locus_tag="ACFM8S_000061"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFC6313210.1"
                     /translation="MRIVEKAYTFDDVLLVPAHSTVLPRDVKLQTKLTREITLNLPLL
                     SAAMDTVTEARLAISMAQEGGIGIIHKNMPPEMQARAVSKVKRHESGVVKDPVTVAPT
                     TLIREVLEMRAQRKRKMSGLPVVENGKVVGIVTNRDLRFENRVDLPVSAIMTPRERLV
                     TVPEGTSIDEARELMHTHKVERVLVLNEKDELKGLITVKDILKTTEFPNANKDSEGRL
                     RVGAAVGTGGDTEERVKALVEAGVDVIVVDTAHGHSQGVIDRVRWVKETYPHIQVIGG
                     NIATAKAALDLVAAGADAVKVGIGPGSICTTRIVAGVGVPQLTAIHNVAEALKGTGVP
                     LIADGGIRFSGDIAKALAAGAYSVMLGGMFAGTEEAPGEIELYQGRSYKSYRGMGSLG
                     AMSQGSADRYFQDKTDSTDKYVPEGIEGRVPYKGPIVNIIHQLTGGLRSSMGYLGCAN
                     IAEMHEKAEFVEITSAGMSESHVHDVQITKEAPNYHR"
     gene            complement(4821..4901)
                     /locus_tag="ACFM8S_000062"
                     /pseudo
     CDS             complement(4821..4901)
                     /locus_tag="ACFM8S_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798803.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(4947..5408)
                     /locus_tag="ACFM8S_000063"
     CDS             complement(4947..5408)
                     /locus_tag="ACFM8S_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313211.1"
                     /translation="MNIIMDSTRKFGILWEENSECNGFIYGKIQIIIGENIYPKICPY
                     GYFTLNAVFNSLKSSFEEKYYAGGNNGLDFGEQLFDIDKYNSLELCNIFSIDTTYMSG
                     GGNCEIDCLVLEMGYSGEEERLFYSFDNGKNFKEIRYKKGTVESVIFQLNL"
     gene            complement(5499..8057)
                     /gene="glnD"
                     /locus_tag="ACFM8S_000064"
     CDS             complement(5499..8057)
                     /gene="glnD"
                     /locus_tag="ACFM8S_000064"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HFC6313212.1"
                     /translation="MPANLSSALETFKQQRDAAEAHYLKANRVSVFFREYTAAVETLL
                     AALWAEYFQNSALCLMAVGGFGRGELYPCSDVDLAVVSPAPLSDGIQEQIARFVQTLW
                     DCKLMPSVKSGGIGELCESVRDDITGDTAFLEARFLFGNRQTADELAKKMNAQRNVAA
                     FVEAKLVEMEHRHAKSQGSGAVLEPNIKSCPGGLRDIHTLLWIAKAQGLATDLPDLLK
                     QRILTRAEAGMLSHGYRRLAHIRIHLHLNAKRAEDRLLFDLQPQVAESMGYEGLNLRR
                     QSEELMRVFYRAIKTVKQLGGILTPMLQSRVSSTPLRVTLRIDDDYIQVNNQIAARHT
                     DIFFRRPEHIFKIVEIMQQRNDITALEPQTLRAWWGATRKINRSFYQNSENRHRFAGF
                     FRNGNGLTQTLRFLNLYGVLGRYLPAWEKIVGLLQHDLFHIYPVDDHILTVVRNVRRL
                     ALDMHSHELPYASALMQSFEKQDILYLAAFFHDIAKGRGGDHAIQGIADARQFAADHF
                     LTEEESDLLAWLVENHLLMSTVAQKEDIQDPSVLDAFCKRVQTHERLSALYLLTISDI
                     RGTNPKLWNAWRASLLESLFHAAGRYLTGNGGNPHTLFGRRRQEAADLLTRAAVPEKQ
                     QKKLWNALGSAYFARHQSREILWHAANLVHDFETPIVRSRILPKSNSFQVMVFMPNGP
                     RLFARLCRIFSRHGFDILAARAFITEHDYILDTFIVQIPSQHAPEDYPDIQSALEAEL
                     NSFIHGHTVAEISSHSRRISRRSRYMPIAPSITITPEEDYPDWYSVEITAVNRPFLLA
                     DMAEVFFAHNVSLRYAKISTLDERAEDSFTVFSLDLKNPKIQSSLKQTLLEQLS"
     gene            complement(8106..8426)
                     /locus_tag="ACFM8S_000065"
     CDS             complement(8106..8426)
                     /locus_tag="ACFM8S_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6313213.1"
                     /translation="MMGNKLTLPAELPDEQDLRAVLAYNMRLFRVNKGWSQEELARQC
                     GLDRTYVSAVERKRWNIALSNIEKMAAALGVAAYQLLLPPQERLNLMTNSADTRQMPS
                     ESGI"
     gene            complement(8751..9224)
                     /gene="bfr"
                     /locus_tag="ACFM8S_000066"
     CDS             complement(8751..9224)
                     /gene="bfr"
                     /locus_tag="ACFM8S_000066"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFC6313214.1"
                     /translation="MKGDRLVIRELNKNLGLLLVTINQYFLHARILKNWGFEELGEHF
                     FKQSIVEMKAADDLIERILFLEGLPNLQELGKLLIGESTEEIIACDLTKEQEKHEALL
                     AAIATAEAQQDYVSRDLLEKQKDTNEEHIDWLETQQELIGKIGLPNYLQTAAQED"
     gene            complement(9252..9716)
                     /gene="bfr"
                     /locus_tag="ACFM8S_000067"
     CDS             complement(9252..9716)
                     /gene="bfr"
                     /locus_tag="ACFM8S_000067"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFC6313215.1"
                     /translation="MQGNQAVVDYMNELLSGELAARDQYFIHSRLYSEWGYTKLFERL
                     NHEMEEETTHAEDFIRRILMLGGTPKMARAELNIGTDVVSCLKADLQTEYEVRDALKK
                     GIKLCEEAQDYVTRDLMVAQLKDTEEDHAHWLEQQLRLIELIGEGNYYQSQL"
     gene            complement(10180..11163)
                     /gene="lipA"
                     /locus_tag="ACFM8S_000068"
     CDS             complement(10180..11163)
                     /gene="lipA"
                     /locus_tag="ACFM8S_000068"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFC6313216.1"
                     /translation="MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAK
                     LPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVG
                     HGRPNMLDPDEPKNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
                     TKIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYKKARPGANYQHSLDLLKR
                     YKEMMPHIPTKSGIMVGLGETDEDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRY
                     VTPEQFKIFEKEAYELGFSNAAIGAMVRSSYHADEQAAEALRESHGGCGHH"
     gene            complement(11156..11779)
                     /gene="lipB"
                     /locus_tag="ACFM8S_000069"
     CDS             complement(11156..11779)
                     /gene="lipB"
                     /locus_tag="ACFM8S_000069"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFC6313217.1"
                     /translation="MKIIHKGLVEYLPTFEAMKTFNAGRDADTEDELWVVEHPPVFTQ
                     GLAGKPEHLLIRDDIPVVQIDRGGQITYHGPGQLVVYTMIDFKRRKTSVRNIVSALEN
                     SIIATLAEYGIEAAADPKRPGVYVGERKIASLGLRIKNGSVYHGLALNVNMDLSPFTH
                     INPCGYAGMEMTQIADFVQPCPTPDEVAAKLTAHLETQFTPKADNNE"
     gene            complement(11781..12056)
                     /locus_tag="ACFM8S_000070"
     CDS             complement(11781..12056)
                     /locus_tag="ACFM8S_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HFC6313218.1"
                     /translation="MTEQENKTSLIEFPCTFPLKVMGAVHPEFEQAVLDTVRLHAPDT
                     QAHHITTRPSSKGNYTGATVQVKVENQEQLDNIYRALTSHELVKVVL"
     gene            12241..13311
                     /locus_tag="ACFM8S_000071"
     CDS             12241..13311
                     /locus_tag="ACFM8S_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFC6313219.1"
                     /translation="MYRRKGRGIKPWMGAGAAFAALVWLVFALGDTLTPFAVAAVLAY
                     VLDPLVEWLQKKGLNRASASMSVMVFSLILLFALLLIIVPMLVGQFNNLASRLPQLIG
                     FMQNTLLPWLKNTIGGYVEIDQASIIAWLQAHTGELSNALKAWFPVLMRQGGNIVSSI
                     GNLLLLPLLLYYFLLDWQRWSCGIAKLVPRRFAGAYTRITGNLNEVLGEFLRGQLLVM
                     LIMGLVYGLGLVLVGLDSGFAIGMVAGILVFVPYLGAFTGLLLATVAALLQFGSWNGI
                     LSVWAVFAVGQFLESFFITPKIVGDRIGLSPFWVIFSLMAFGQLMGFVGMLAGLPLAA
                     VTLVLLREGVQKYFAGSFYRGR"
     gene            complement(13417..14364)
                     /locus_tag="ACFM8S_000072"
     CDS             complement(13417..14364)
                     /locus_tag="ACFM8S_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HFC6313220.1"
                     /translation="MEFFIILLAAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGL
                     NILIPFIDRVAYRHSLKEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSN
                     YIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVSALDEAAGAWGVKVLRYEIKD
                     LVPPQEILRSMQAQITAEREKRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAV
                     NASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVA
                     AFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAK"
     gene            complement(14379..14786)
                     /locus_tag="ACFM8S_000073"
     CDS             complement(14379..14786)
                     /locus_tag="ACFM8S_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFC6313221.1"
                     /translation="MTVWFVAAVAVLIIELLTGTVYLLVVSAALAGSGIAYGLTGSTP
                     AAVLTAALLSALGIWFVHAKTAVGKVETDSYQDLDAGQYAEILRHAGGNRYEVFYRGT
                     HWQAQNTGQDVFEPGTRALIVRKEGNLLIIAKP"
     gene            14867..15526
                     /gene="ung"
                     /locus_tag="ACFM8S_000074"
     CDS             14867..15526
                     /gene="ung"
                     /locus_tag="ACFM8S_000074"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFC6313222.1"
                     /translation="MDTWHDALGNEKRQPYFQEILNAVRQERLSGQIIYPPAEDVFNA
                     FRLTAFDRVKVVILGQDPYHGAGQAHGLAFSVRQGIRIPPSLLNIYKELETDINGFSI
                     PDHGCLTAWAEQGVLLLNTVLTVRAGQAHSHALLGWERFTDTVIRQLAAHRKHLVFML
                     WGGYAQQKRKLIDSQNHLILTAPHPSPLSAYRGFFGCRHFSQANSYLSRHGIDPINWK
                     L"
     gene            15674..16790
                     /locus_tag="ACFM8S_000075"
                     /pseudo
     CDS             15674..16790
                     /locus_tag="ACFM8S_000075"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
     gene            16778..17092
                     /locus_tag="ACFM8S_000076"
     CDS             16778..17092
                     /locus_tag="ACFM8S_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HFC6313223.1"
                     /translation="MVHLSEIAAQDYNLNLPRYIDSGEVEDLQNLAAYLYGGIPAHDM
                     DALEAYWQVLGRMKNELFAEHDGRFTTIQRNRLQIFPTLNSLKSCGIGVEFRANLVQP
                     QI"
     gene            17134..18078
                     /locus_tag="ACFM8S_000077"
     CDS             17134..18078
                     /locus_tag="ACFM8S_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFC6313224.1"
                     /translation="MAHPDYAAFKAGHLAKFAAWHTQNDLAAIQPGRLIRKWSESLLD
                     AFKPGSLIEEYDFYQILTDYWAETLQDDVYLIAQDGWKAVKNLAEITKESDEDANLTV
                     VFEETETGKKGKAKTKRISKKYRSEVIAPELVARRYFSDGIAKLEEKQSELERLSQEL
                     ENHIEEHGGEEGALNDVLDAKGKLSAKLLKTALEESGIEEGERAVLQTTQTLMTQEKA
                     AKDAVKTQIEALNLAVFKQFGRLSEAEIKQLAVQDKWLADLQSRIENRLENSIQQLIS
                     RLNTLEDRYRSPMAELAREVEKWQSKVNAHLENMGFGG"
     gene            18229..18354
                     /locus_tag="ACFM8S_000078"
     CDS             18229..18354
                     /locus_tag="ACFM8S_000078"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313225.1"
                     /translation="MLERHRNARFMAHMDNFLPNWQSIKQQLNALELFAQIYNLT"
     gene            complement(18370..18594)
                     /locus_tag="ACFM8S_000079"
     CDS             complement(18370..18594)
                     /locus_tag="ACFM8S_000079"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313226.1"
                     /translation="MMKKQRHILCVGCLYIVLKVNVFPNTGWMEASKGMQGISFSTFG
                     YADAPGYTFSRIVHTLPTWKGHRDTYNCFR"
     gene            complement(18639..19154)
                     /locus_tag="ACFM8S_000080"
     CDS             complement(18639..19154)
                     /locus_tag="ACFM8S_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313227.1"
                     /translation="MKSKLPLILINFSLISSPLGANAAKIYTCTINGETVYTTKPSKS
                     CLSTDLPPIGNYSSERYIPPQTSEPTPSPSNGGQAVKYKAPVKTVSKPAKSNTPPPQQ
                     APSNNSRRSILETELSNERKALVEAQKMLSQARLAKGGNINHQEINALQSNVLDRQQN
                     IQALQRELGRM"
     gene            complement(19620..21542)
                     /locus_tag="ACFM8S_000081"
     CDS             complement(19620..21542)
                     /locus_tag="ACFM8S_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC6313228.1"
                     /translation="MIEIKNLTLQRGLKVLLDKANATVNPGQRVGLIGKNGTGKSSLF
                     ALIKGEITQDGGDVSIPKNWRLASVSQETPDLDISALDYVLQGDAELQTLQTALRQAE
                     AQNDGMKQAEYHAKLEEIDAYTAPARAAKLLNGLGFSQEEHSRPVKSFSGGWRMRLNL
                     AQALICRADLLLLDEPTNHLDLETVLWLENHLASLPCTQIIISHDRDFLNAATTQTIE
                     LSQQKLTQYGGNYDFYQNERAQRLAQQQAAYVKQQAQIKHLQSFIDRFKAKATKAVQA
                     QSRMKALAKLERIAPAHLDSEFSFEFYNPEHLPNPLLKLEHADLGYEGKTVLHDITLS
                     LESGARYGLLGVNGSGKSTFIKALAGKIDLLSGSIVRSEKLNIGYFAQHQLDTIRADQ
                     NPVWHIQQLSPEVREQEIRNFLGGFNFVGDMALQKTEPFSGGEKARLALAMIIWQKPN
                     LLLLDEPTNHLDLDMRHALTLALQSFQGALIAVSHDRSLLEATTDSFLLIDKGRLKNF
                     DGDLNDYRQWRLAQENAAAAPAASAQSQSRKDTKRIEAQIRQEKARRGKPIQQKIDRA
                     EKEMAQLSEIQTACEAFLAQEDAYLEANKEKLQNTLSLLTKVKTQLAQIEEVWLACQE
                     ELERIETEIEKQFAER"
     gene            complement(21619..21990)
                     /locus_tag="ACFM8S_000082"
     CDS             complement(21619..21990)
                     /locus_tag="ACFM8S_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HFC6313229.1"
                     /translation="MLFEENPIDGHFTEYGHRSGEIRLAGQSFHKPVLVHKDSVCLSQ
                     CRTLSDLTPENLLSDIKPVDYPEILIIGTGAAQEFIHPKIMADFSRIGISVECMNTDS
                     AFRTLVFLHSEGRRAWAWLQP"
     gene            22187..23494
                     /locus_tag="ACFM8S_000083"
     CDS             22187..23494
                     /locus_tag="ACFM8S_000083"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HFC6313230.1"
                     /translation="MKPVNIGLLGLGTVGGGTAAVLRDNAEEISRRLGREIRISAVCD
                     LSEEKARQTCPSAAFVKDPFELVAREDVDVVVELFGGTGIAKDAVLKAIENGKHIVTA
                     NKKLLAEYGNEIFPLAEKQNVIVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGT
                     SNFILSEMREKGSAFADVLKEAQALGYAEADPTFDIEGNDAGHKITIMSALAFGTPMN
                     FSACYLEGISKLDSRDIKYAEELGYRIKLLGITRKTGKGIELRVHPTLIPESRLLANV
                     NGVMNAVRVNADMVGETLYYGAGAGALPTASAVVADIIDIARLVEADTAHRVPHLAFQ
                     PAQVQAQTILPMDEITSSYYLRVQAKDEPGTLGQIAALLAQENVSIEALIQKGVIDQT
                     TAEIVILTHSTVEKHIKSAIAAIEALDCVEKPITMIRMESLHD"
     gene            23487..23921
                     /locus_tag="ACFM8S_000084"
     CDS             23487..23921
                     /locus_tag="ACFM8S_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313231.1"
                     /translation="MTEPKHEMPTEEQVAARKKAKAKIRTIRIWAWVILALLALTALL
                     SQCAMSKPQAKQKIVESCVKNIPFAEKWQNDLRARGLDSNNTRLAVDYCKCMWEQPLD
                     RLSEKQIRSFGKLGAQEQLDLLGGANAFEARDKQCVADLKSE"
     gene            complement(23980..24249)
                     /locus_tag="ACFM8S_000085"
     CDS             complement(23980..24249)
                     /locus_tag="ACFM8S_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFC6313232.1"
                     /translation="MNKSELIEAIAQEADISKAAAQKALDATTNAVTTALKQGDTVTL
                     VGFGTFYVGERAERQGRNPKTGEPLTIAAAKTPKFRAGKALKDAL"
     gene            complement(24431..26881)
                     /gene="lon"
                     /locus_tag="ACFM8S_000086"
     CDS             complement(24431..26881)
                     /gene="lon"
                     /locus_tag="ACFM8S_000086"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFC6313233.1"
                     /translation="MTQKEKHFEEYAALATLPLRDVVVYPHMVLPLFVGRPKSIAALE
                     NAITREEPVFLLAQTDAAVEEPVAADLYQTGTVAQVLQVLKLPDGTVKVLVEGLYRGR
                     VLTIEDTGGLFVSHIETVVEEDTGGNTDLEAVRRTLLAQFEQYAKLNKKIPAEIIGSI
                     NGIAENSRLTDTVAAHLQLKLAQRQQILEIPEIGKRMEFLLAQLESELDIMQAEKRIR
                     GRVKRQMEKSQREYYLNEQIKAIHKELGEEDENGELDALEAGIKKAGMSKEAEEKCLS
                     ELKKLKMMPPMSAESTVVRNYIDTLLELPWKKKSRVSKDIAKAGLVLDTDHYGLEKVK
                     ERILEYLAVQKRMDKLKGPILCLVGPPGVGKTSLGESIAKATGRKYVRMALGGVRDES
                     EIRGHRRTYIGSMPGKILQNMAKAGVKNPLFLLDEIDKLGSDFRGDPASALLEVLDPE
                     QNNKFADHYAEVDYDLSDVMFIATSNSLNIPTPLLDRMEIIRLSGYTEDEKINIAMQY
                     LVPKQMKRNGVKEGELAIEESAVRDIIRYYTREAGVRSLDREIAKICRKVVMQITLDE
                     DSKTKKTGKAKPRAVKVNEKNLHDYLGVRRFDYGVAESENRIGQVTGLAWTEVGGELL
                     TVEAAALPGKGVIQCTGQLGDVMKESVSAAWSVVRSRAESVGLAPDFYEKKDIHVHVP
                     EGATPKDGPSAGIAMTLAMVSAFTKIPVRADVAMTGEITLRGEVLPIGGLKEKLLAAL
                     RGGIKHVLIPKDNLKDLEEIPENVKTGLTIHPVKWIDEVLALGLESQPEPWAEPSGAE
                     AAAESASKPKPRSRATKH"
     gene            26981..27157
                     /locus_tag="ACFM8S_000087"
     CDS             26981..27157
                     /locus_tag="ACFM8S_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase"
                     /protein_id="HFC6313234.1"
                     /translation="MPSETFRLQTAWAWKDKAGTKNCSVLPLLAVPSVWFAVLPPYFQ
                     NMTQVFHVFFYFSR"
     gene            27132..28235
                     /locus_tag="ACFM8S_000088"
     CDS             27132..28235
                     /locus_tag="ACFM8S_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFC6313235.1"
                     /translation="MSSFIFPDNGFEWRNESLQKPPKGWHYVRESGANGILKAAYQNI
                     ATVWQGDFHNAKQVLSAMKKKVRKPAAVRSDADIAALFHAHRMKQAQQSRILNMLAVE
                     IRPGFVLDNKRAPDIRAALLDVYGEADGKPFFLPLNLLLGFMGAHEWHKKGVAVPQLG
                     GSIHVPFGVFSPLRGEYLDLLAHAPSTGFQTAFDIGTGSGVLAAILAKQGIPSVIGTD
                     TNPKAVACARANIARLGFEKQVEIRETDLFPEGFADLIVCNPPWLPAKPTSAVESALY
                     DPESAMLAAFLRDAPKHLNPDGEIRLIISDLAEHLGLRPADFLEKAFIRAGLRVADMM
                     KTKPKHKKAANPSDPLAFARTRETTFLYRLKKA"
     gene            complement(28263..30008)
                     /gene="recD"
                     /locus_tag="ACFM8S_000089"
     CDS             complement(28263..30008)
                     /gene="recD"
                     /locus_tag="ACFM8S_000089"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFC6313236.1"
                     /translation="MERQTDEFAQAAARAAIWFLARYAGSSDEVLANCTERLFQALQN
                     GHSFIRLSGGEADALSALAPVVGTSAAPLILEGRRLFLGRMWQLEYDLAAEIKRLAAA
                     DTSAPDAAGARQNLAKWFQGTGSEGQRDAAALALLQSFMVITGGPGTGKTTTVAKLLA
                     LICGEDENLPNIALAAPTGKAAAHMARALHRAINGFDAPEAVRRHLLKLEGQTVHRLL
                     KLRPPKMQAAFNPVYPLPFDVLVIDEASMLDTVLMLQLLKAVKTGARVILLGDENQLP
                     SVGIGDVLSVLSQKTVLDGETHQRLAGFLPEHGFSVSANPPLLAQNTAHLSFSHRFGD
                     NSGIGCLARAAVSGDEGAWALFDRFPDELEHSECSPNARVERLYRAHKAYWQAVKDGN
                     IEAAYAGISDIVVLAAWRQDAEDFNEAYCSHVRRKMNIPEHQAYFAGEPIMIRQNDYA
                     LELFNGDIGLIMEDVGRQGGLAAYFADADGFKKVAVSCLPDFEPAFAMTVHKSQGSEY
                     REVWLLPPSAAPSDEGDDALSGLSRGLLYTAITRAREKFVFFGGEETFRQAAATVKTR
                     QTALGSMLERVFSQE"
     gene            complement(30076..30771)
                     /gene="lolD"
                     /locus_tag="ACFM8S_000090"
     CDS             complement(30076..30771)
                     /gene="lolD"
                     /locus_tag="ACFM8S_000090"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFC6313237.1"
                     /translation="MSELILKCESVGKRYRDGGLDVPVLHGLDLEIRAGESTGIIGSS
                     GSGKSTLLHILGGLDMPSEGRVLLMGEDLRTLNQRRLGDLRNRHLGFVYQFHHLLPEF
                     SALENVMMPLLIGKKSREEAAETAMAMLEKVGLKHRSTHRAGELSGGERQRAAIARAL
                     VTQPKCLLADEPTGNLDRANARNVLDMMLELKTELGTGLVVVTHDDELAGRFGRVMVM
                     KDGSLHPKQGANA"
     gene            complement(30764..32011)
                     /locus_tag="ACFM8S_000091"
     CDS             complement(30764..32011)
                     /locus_tag="ACFM8S_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HFC6313238.1"
                     /translation="MFSLEAWIGLRYLRAKKRNGFMSFITMVSIAGIALGVTALIVVL
                     SVMNGFQKEIRGQLLNVAPHAEIGYIDNTDTDWRNLLRFTENRKGILAAAPYVSNQAL
                     LANAGEIRGVQMRGILPSEERKVVEYGDKMPAGKFEDLIPGEFDIILGVGLAEALGAE
                     VGNKVTVITPEGNVTPAGVVPRLKQFTVVGLVKTGVYEVDNSLAMTHIQDARVLYRLD
                     KEVAGLRLKLADPQNAPALTATLIPEAQRDAVWVRDWTYNNRSYFEAVELEKRMMFII
                     LTLIIAVAAFNLVSSLVMAVTEKQADIAILRTLGLSPGGVMKIFMVQGAFSGFFGTLA
                     GVVCGVLLGWNVGRVVAFFENLLGVHLINSQVYFIDYLPSDVDMGDVALIACISLGLS
                     FVATLYPSRRASKTQPAEALRYE"
     gene            32216..32494
                     /locus_tag="ACFM8S_000092"
     CDS             32216..32494
                     /locus_tag="ACFM8S_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313239.1"
                     /translation="MNIRTAFALCAIALSAAAAAYAKEIKIDANNTPYSEADAQKLAA
                     TAVGMGVKEPISLNGGSGSITVSGSSATQCVFKVGNGGALQIQGLNCK"
     gene            complement(32550..33167)
                     /gene="recR"
                     /locus_tag="ACFM8S_000093"
     CDS             complement(32550..33167)
                     /gene="recR"
                     /locus_tag="ACFM8S_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFC6313240.1"
                     /translation="MSHKKQDAFQGLIDALKVLPNVGPKSAQRIAYHLLQHKRKEAEK
                     LVDALQTALKQVYHCAMCNTFCEGGLCDICADETRDGRRLMVVHMPADVSNMEAANCH
                     DGLYFVLMGQINTALGMDVSAIALDRLAQRLGGGEVEEIIIATAFTAEGNATAYVLSE
                     FFKNLPYKVSRLSQGIPLGGELEYVDAGTLAQAVYERRLIKEGGA"
     gene            complement(33234..34772)
                     /locus_tag="ACFM8S_000094"
     CDS             complement(33234..34772)
                     /locus_tag="ACFM8S_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HFC6313241.1"
                     /translation="MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGD
                     EKISDHSINNAIQNEQADGGGPSRDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQIIV
                     DDPNFHDANGKFDHALLNRYLSQRHMSEDQFVEEIRDQFALQNLVNLVQNGVLVGDAQ
                     AEQLIRLTQVNRTIRSHTFNPDEFIAQVKVSEADLQKFYNANKKDYLLPKAVKLEYVA
                     LNLKDFADKQTVSETEVKNAFEERVARLPANEAKPSFEQEKAAVENELKMKKAVADFN
                     KAKEKLGDDAFNHPSSLAEAAKNSGLKVETQETWLSRQDAQMSGMPENLINAVFSDDV
                     LKKKHNSEVLTINSETAWVVRAKEVREEKTLPFAEAKDAVRQAYIRTEAAKLAENKAK
                     DVLTQLNGGKAVDVKWSEVSVLGAQQARQSMPPEAYAELLKAKPANGKPAYVRLIGLP
                     APVIVEVQAVTPPDDIAAQLPLAKQALAQQQSANTFDLLIRYFNGKIKQTKGAQSVDN
                     GDGQ"
     gene            complement(34832..35206)
                     /locus_tag="ACFM8S_000095"
     CDS             complement(34832..35206)
                     /locus_tag="ACFM8S_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HFC6313242.1"
                     /translation="MIILHGIPNCDTVKKAKNRLAGYGLEFEFRDFKKQVPSEAEICS
                     WLEQVPLATLLNKRGTSWRKLDAETQQKALFSTAEAVKLMSEMPSLIKRPVLECGGKV
                     YAGFSEETYDGIFNRQAPCRQG"
     gene            35356..36984
                     /locus_tag="ACFM8S_000096"
     CDS             35356..36984
                     /locus_tag="ACFM8S_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFC6313243.1"
                     /translation="MISTNGITMQFGAKPLFENVSVKFGEGNRYGLIGANGSGKSTFM
                     KILGGDLEQTAGEVAIENGVRLGKLRQDQFAYEDMRVLDVVMMGHTEMWAAMTERDAI
                     YANPEATEDDYMKAAELEAKFAEYDGYTAEARAAELLSGVGISEDLHNATMAEVAPGF
                     KLRVLLAQALFSKPDVLLLDEPTNNLDINTIRWLEGVLNQYDSTMIIISHDRHFLNEV
                     CTHMADLDYNTITIYPGNYDDYMLASAQSRERALKDNAKAKEKLQELQEFVARFSANK
                     SKARQATSRLKQADKIKSEMVEVKPSTRQNPYIRFEADEKAKLHRQAVEVEKLAKRFE
                     TQLFKNLNFILEAGQRLAIIGPNGAGKSTLLKLLAGAYNPEYSDGLLPDEGSIKWAEK
                     ASVGYYPQDHENDFDVDMDLSEWMRQWGQDGDDEQVIRGTLGRLLFGSNDVVKKVKVL
                     SGGEKGRMLYGKLLLLKPNVLVMDEPTNHMDMESIESLNMALEKYNGTLIFVSHDRQF
                     VSSLATQIIELDGKGGYEHYLGDYESYLEKKGVA"
     gene            37056..38309
                     /locus_tag="ACFM8S_000097"
     CDS             37056..38309
                     /locus_tag="ACFM8S_000097"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HFC6313244.1"
                     /translation="MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGK
                     DFPVFLHPETHEEYALARTERKTAKGYVGFSFHADKDVTLEQDLMRRDLTINAMAQDA
                     DGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRTARFAARYKFEIAEETIKLMRQM
                     VENGEADALVAERVWQEFAKGLMEKNPRKMIEVLRECGALKVLLPEVNALFGVPQRAD
                     YHPEIDSGIHTLMTLQRAADMGLSLPERYAALLHDLGKAKTPSDILPRHHGHDLAGVE
                     PVREVNQRLRAPKHCAELAELVCRWHIIFHQVGQLKSQTILNVLKKTDAFRRPERFQT
                     ALNVCIADTQGRLNREHTPYPQRAHWLALLEAANQADSGKIAAECRSQGKAHLIAEQI
                     DRARLAQIAPLQKAFRAAQDKTEKH"
BASE COUNT         8662 a         9850 c        10294 g         9520 t
ORIGIN      
        1 taccgcaaaa cataccacaa aaaacggtaa cggatagttg taagcggttg atgacgatta
       61 tagacaataa cgggttttcc caatgaaatt attgtttgaa taataaaaaa tcccaaaccg
      121 taaaagtttg ggatttgtat ttggcagaga ggaagggatt cgaaccctcg atacgctatt
      181 cacgtataca cgctttccag gcgtgcgact taaaccactc atccacctct ctaatggcgg
      241 aaattatccc aatcggaata atttatttgg tgcccgggag aagactcgaa cttccacacc
      301 cgtgaggata ccagcacctg aagctggcgc gtctaccaat tccgccaccc gggctttgct
      361 ttatttgctg ttttgcggta cagtgtcctg ccgcaaagaa gtcgctatta tatagttcat
      421 aaagagaatg tcaacagtcc aaatgaataa aaatattaaa tctttaaatt tacgggaaaa
      481 agacccgttt ttaagtcgtg aaaaacagcg ttatgaacat cctttgccca gtcgggaatg
      541 gataatcgaa ttgttggagc gcaaaggtgt gccttcaaaa atcgaatcgc ttgcgcgcga
      601 gctgtcgatt acggaagacg agtatgtctt tttcgaacgc cgtctgaagg cgatggcgcg
      661 ggacggtcag gttttaatca accgccgggg cgcggtttgc gcggcggaca aattggattt
      721 ggtcaaatgc cgtgtcgagg cgcacaaaga cggtttcggt tttgccgtgc cgctcacgcc
      781 cgccaaagac ggtgattttg ttttgtatga acgccagatg cgcggcatta tgcacggcga
      841 tattgtcact gttcgtcctg ccggcatgga ccgtaggggc cgccgcgaag ggacagttct
      901 ggatattgtc gaacgcgcgc aaagcaaagt ggtcggccgt ttctatatgg ataggggcgt
      961 ggcgattttg gagccggaag acaagcgtct gaaccaaagc atcgtattgg aaccggacgg
     1021 cgtggcgcgt ttcaaacctg aatccggtca ggtcatcgtc ggcgaaattg aggtttatcc
     1081 tgagcaaaac cggccggcag tggcaaaaat catcgaagtt ttgggcgatt atgccgacag
     1141 cggcatggag attgaaattg ccgtgcgcaa gcatcatttg ccgcaccaat tcagtgaagc
     1201 gtgtgccaaa gccgcgaaaa aaattcccga ccatgtacgc aaaagcgatt tgaaaggccg
     1261 cgtcgatttg cgcgatttgc ctttggtaac gatagacggc gaaacggcgc gcgattttga
     1321 cgatgccgtg tttgccgaga aaatcggacg caattaccgt ttggtcgtgg cgattgccga
     1381 tgtcagccat tatgtccgcc ccgatgacgc tatcgacacg gacgctcagg aacgcagcac
     1441 cagtgtttac ttcccgcgcc gcgtgattcc catgttgccg gaaaacctgt ccaacggcat
     1501 ctgctcgctc aatcctaatg tcgagcgttt gtgtgtggtg tgcgatatgg ttatcactta
     1561 cgcgggcaat atcaaagaat accgcttcta ccccgccgtg atgcgctctc atgcccgcct
     1621 gacctacaac caagtttgga aatggctttc aggcggcatc gggcatccgt tcaaaaccca
     1681 aatcgacacg ctttacaaac tcttcaaaat ccttcagaaa aagcgtttcg aacgcggggc
     1741 ggtggagttt gacagcatcg aaacccaaat gcttttcgac gacaacggta aaattgaaaa
     1801 aatcgtcccc gttgtccgca acgatgccca caagctgatt gaagaatgta tgctggcggc
     1861 gaatgtttgc gcggcggatt ttctgttgaa aaacaagcat acggctttgt tccgcaacca
     1921 tttgggcccc acgcccgaaa aactcgccac cctgcgcgag cagctcggtc tgttggggct
     1981 tcaacttggc ggcggcgaca acccgtcgcc gaaagactat gccgcgcttg tcgaacaatt
     2041 caaaggcaga cctgatgccg aattgctgca agtcatgatg ttgcgctcca tgcagcaggc
     2101 ggtttacgaa ccgcattgcg acggacactt tggtcttgcc tacgaagcat acgcccactt
     2161 cacctcgccc atccgccgct atcccgacct gaccgtacac cgcgccatca aagccgtgtt
     2221 gaatcagcaa acctacacgc caaaaaaaag ctggcaggct ttgggcgtgc atacctcgtt
     2281 ctgtgagcgc cgtgccgacg acgccagccg cgacgtggaa aactggctga aaacctatta
     2341 tatgcgcgat aaggtcggcg aagtattcga aggtaaaatc tccggcatga ccagttttgg
     2401 tatctttgta acactggacg gcatccacat tgacggcttg gtgcatatca gcgatttggg
     2461 cgaagactat ttcaacttcc gccccgaaat catggcaatc gaaggcgaac gcagcggcat
     2521 ccgtttcaac atgggggaca gggttgccgt ccgggtcgcc cgtgccgatt tggatgacgg
     2581 aaaaatcgat tttgtcctga ttgccggggg gagcggcagg gggcggaaag ttaaatcatc
     2641 cgcgtctgcc aaaccggcag ggacggcggg gaaagggaag ccgaaaaccg ccgccgagaa
     2701 aaaaacagcc cgaggcggca aagtaagagg aaggggcgcg tctgccgccg cagaatcgag
     2761 gaaaaaggca aagaaaccgg ttccgattaa ggtaaaaaaa cggaaaggca aatcataatg
     2821 ctgacggggc ggcttgagga ggcggggcat attttcccga agcctccacc cgattttttg
     2881 atgtgggcgg acggcgcatc atttcaggcc aatcgaaacg cccgtattga aagattgcgt
     2941 ttatttccac cgccgtttta aaggccagcg gtattcggca gacggggcgc aaacggcgtt
     3001 cagacggcat tttcattctt tcggcgtgtc cgtccgaatt gctttgcccg tccgcgcaat
     3061 cagcccgtgc ggcttgcctt gaacggacaa aaaatgccgt ctgaaacccg aaaatcaggt
     3121 ttcagacggc atttttcctt gaaaaggctg ttcaaatcag cgatggtagt tcggtgcttc
     3181 tttggtaatt tgaacgtcgt gaacgtgcga ttcgctcata cctgcggaag tgatttccac
     3241 aaattctgct ttttcgtgca tttcagcaat attggcgcaa cccaaatacc ccatgctgga
     3301 gcgcagtccg ccggtcagtt ggtggatgat gttcacaatc ggacctttgt aaggaacgcg
     3361 gccttcgatg ccttcgggga cgtatttgtc ggtgctgtcg gttttgtctt ggaagtagcg
     3421 gtcggcagaa ccttggctca tcgcgcccaa ggaacccata ccgcgatagg atttgtacga
     3481 gcggccttgg tagagttcga tttcgcccgg tgcttcttcc gtgcctgcaa acataccgcc
     3541 gagcatgacg ctgtacgcgc ctgcggcgag ggctttggcg atgtcgccgg agaagcggat
     3601 gccgccgtcg gcaatcagcg gaacgcccgt gcctttgagg gcttcggcaa cgttgtggat
     3661 ggcggtcagt tgcggcacgc ccacgcctgc cacgatacgg gtggtgcaaa tcgatcccgg
     3721 accgataccg actttgacgg catccgcgcc ggcagcgacc aaatccaaag cggctttggc
     3781 agtggcgatg ttgccgccga tgacttggat gtgcggatag gtttctttga cccaacgcac
     3841 gcggtcgatc acgccttggc tgtgcccgtg ggcggtatcg acgacaatca cgtccacgcc
     3901 ggcctcaacc aaggctttga cgcgctcttc ggtgtcgccg ccggtgccga ctgccgcacc
     3961 gacgcgcaga cggccttcgg agtctttgtt ggcattggga aactcggtgg tttttaaaat
     4021 atctttgacg gtaatcagac ctttgagttc gtctttttcg ttcagaacca aaacgcgctc
     4081 gactttgtgc gtgtgcatca gttcgcgcgc ttcgtctatg cttgtgcctt cggggacggt
     4141 aaccagacgt tcgcgcgggg tcataatggc ggaaacgggc aaatcgacgc ggttttcaaa
     4201 acgcaggtcg cggttggtta cgatgccgac gactttgccg ttttcaacga cgggcaggcc
     4261 ggacattttg cgtttgcgct gcgcacgcat ttccaagact tcgcggatga gcgttgtcgg
     4321 tgcaacggtt acggggtctt tgaccacgcc gctttcgtgg cgtttcactt tggaaacggc
     4381 gcgcgcctgc atttcgggcg gcatgttttt atggatgatg ccgatgccgc cttcttgtgc
     4441 catcgaaatg gcgaggcgcg cctcggtaac agtgtccatc gcggcggaaa gcagggggag
     4501 gttgagtgtg atttcgcggg tgagcttggt ttgaagttta acgtctcgcg gcagcacggt
     4561 cgaatgtgcg ggaaccaaca aaacatcgtc gaaagtatag gctttttcta cgatacgcat
     4621 aatgctcggt ctttcagttt gtgcaagatg cacggcatta tagcacgtta ccggcggctt
     4681 gacagtttat caggtttaat tttggtcccc tttgaatagc ccggttttcc tttgccgacc
     4741 actgttgctc ccgttctttc aatttcagga aaagcttttt tctaattttt ggtaagtggc
     4801 tcagttattg aagccctata tcgggcggta acttccagca caaaaaaacg gagtagttct
     4861 ttatttattt tttcctttaa ttttcagtat attatcttaa tatttcgagg gtaacatatc
     4921 tgctaatcta gttacagccc catatattat agattcaatt gaaaaataac agattcaact
     4981 gtacctttct tatacctgat ttctttaaag tttttcccat tgtcaaaact ataaaaaagt
     5041 ctctcttcct caccagaata tcccatttct aatactaaac aatctatttc acaattaccc
     5101 cctccactca tatatgtagt atcaatagaa aatatattgc ataattccag agaattatat
     5161 ttatctatat caaatagttg ctctccaaaa tctaaaccat tgtttccacc tgcataatac
     5221 ttctcttcaa aggatgactt caaactatta aaaactgcat ttagcgtaaa atatccatat
     5281 gggcatatct taggatatat attctctcct attattatct gaatttttcc ataaataaaa
     5341 ccattacatt cagagttttc ttcccataaa atcccaaatt ttcttgttga gtccataata
     5401 atattcatat aaatccttat attaataaat tatttacaat atcccccgct ttcagacggc
     5461 atacggcgtg gcggcggaat gccgtctgaa ggcgggcgtt atgataattg ttccagcagc
     5521 gtctgcttca atgaggactg gatttttggg tttttcaggt ctaggctaaa aacggtaaaa
     5581 ctgtcttcgg cgcgttcatc cagtgtggag attttggcat agcgcaggct gacgttgtgg
     5641 gcgaaaaaga cttccgccat atcggcgagc aggaaggggc ggttgacggc ggtgatttcg
     5701 acggagtacc aatcggggta gtcttcttcg ggggtgatgg tgatgcttgg cgcgatcggc
     5761 atatagcggc tgcggcggct gatgcggcgg ctatggctgg agatttcggc aacggtgtgt
     5821 ccgtggataa agctgttgag ttcggcttcg agcgcgcttt ggatgtcggg gtagtcttcg
     5881 ggagcgtgct gcgaggggat ttgcacgatg aaggtgtcga ggatgtagtc gtgttcggtg
     5941 ataaaggcgc gggcggcgag gatatcgaag ccgtggcggc tgaaaatgcg gcagaggcgg
     6001 gcgaacaggc gcgggccgtt gggcatgaaa accatgactt gaaagctgtt gcttttgggc
     6061 aggatgcggc tgcggacgat gggggtttca aagtcgtgga ctaggttggc ggcgtgccac
     6121 aggatttcgc gggactggtg gcgggcgaag taggcggaac cgagcgcgtt ccatagtttt
     6181 ttctgctgtt tttcggggac ggcggcgcgg gtgagtaagt cggcggcttc ctgccggcgg
     6241 cggccgaaga gggtgtgcgg gttgccgccg ttgcctgtaa ggtagcgtcc ggcggcatgg
     6301 aagaggcttt ccagcaggct ggcgcgccat gcgttccaca gcttgggatt ggtgccgcgt
     6361 atgtcggaaa tggtcagaag gtagagcgcg ctgaggcgtt cgtgggtttg gacgcgtttg
     6421 cagaaggcat cgagtacgct ggggtcttgg atgtcttctt tttgggcgac ggtggacata
     6481 agaagatggt tttcaaccag ccaggcgagc aggtcgcttt cttcttcggt caggaagtgg
     6541 tcggcggcaa attggcgcgc gtctgcgatg ccttgtatgg catggtcgcc gccgcgtcct
     6601 ttggcgatgt catggaaaaa ggcggcaagg tagaggatgt cttgtttttc aaaggactgc
     6661 atcagtgcag aggcgtaggg cagctcgtgg ctgtgcatat ccaaggcaag gcggcggacg
     6721 ttgcggacga cggtgaggat gtggtcgtcc acgggataga tgtggaacag gtcgtgctgg
     6781 agcaggccga cgattttttc ccacgcgggc aggtagcgtc ccaatacgcc gtagaggttg
     6841 aggaagcgca gggtttgggt cagtccgttg ccgttgcgga aaaagccggc gaagcggtgg
     6901 cggttttcag aattttggta gaagctgcgg ttgattttgc gcgtcgcgcc ccaccatgcg
     6961 cgcagggttt gcggttcgag cgcggtaata tcgttgcgct gctgcatgat ttcgacgatt
     7021 ttgaaaatgt gttcgggccg tctgaaaaaa atatcggtgt gccgcgcggc aatttggttg
     7081 ttgacttgga tgtagtcgtc gtcaatccgc agggtaacgc gcaacggggt ggaggaaacg
     7141 cggctttgca gcataggcgt gaggatgccg cccagttgtt tgacggtttt aatcgcgcgg
     7201 taaaacacgc gcatcagttc ttcgctttgg cggcggaggt tcaagccttc ataacccatg
     7261 ctttcggcga cttgcggctg caaatcgaac agcaggcggt cttcggcgcg cttggcgttt
     7321 aaatgcagat ggatgcggat gtgggcgagg cggcggtagc cgtgcgaaag cataccggct
     7381 tcggcacgcg tcaaaatccg ctgtttgagc aggtcgggca ggtcggtcgc caagccttgc
     7441 gccttcgcta tccaaagcag ggtgtggatg tcgcgcagac cgcccggaca gcttttgata
     7501 ttcggctcca atactgcccc cgaaccttgc gatttggcgt ggcggtgttc catctccacc
     7561 agttttgcct cgacaaacgc cgccacattg cgctgcgcgt tcattttttt cgccagttcg
     7621 tccgccgttt ggcggttgcc gaacaaaaaa cgggcctcta aaaacgccgt gtcccccgta
     7681 atatcgtcgc gcacgctttc gcacagttcg ccaatgccgc cgctttttac agacggcatc
     7741 agtttgcagt cccatagggt ttgaacaaac cgggcaatct gttcctgaat gccgtctgaa
     7801 agcggggcag gggagacaac cgccaaatcc acatccgaac agggatacag ttcgccgcgc
     7861 ccgaagccgc ccaccgccat caggcataac gcgctgtttt gaaaatactc tgcccacaat
     7921 gccgccagta aggtttcgac tgccgccgtg tattctctga aaaataccga cacgcggttg
     7981 gctttcaaat aatgcgcttc ggcggcatcg cgctgctgtt tgaaggtttc cagtgctgaa
     8041 gacaggtttg caggcatttt tattctttcg attggcggga aaaaaggaga cggatggttc
     8101 ggcggtcaaa taccgctttc agacggcatt tgtcgggtat cggcggaatt ggtcatcagg
     8161 ttcaaccgtt cctgcggcgg cagcagcaac tgatacgccg ccacgcccaa agccgccgcc
     8221 attttttcga tattcgacag ggcgatgttc cagcgtttgc gttcgactgc cgacacataa
     8281 gtcctgtcca aaccgcattg ccgcgccaat tcttcttgcg accaaccctt gttcacgcgg
     8341 aaaagccgca tattgtatgc caataccgcc cgcaaatcct gttcgtcagg cagttcggca
     8401 ggcagagtca atttgttgcc catcatttgc ttccaaataa atggttaaag ttaagcattt
     8461 gctgttacgg attttacttc acataaaagc caaaaaaaag gggtggcggc agccacaccc
     8521 aaacacacac acatcaagag gaaagaggta acatcaagac agattgggaa acagcaaaca
     8581 agcaatctag atttcatatc ggtattatca aacaaaaacc gttatcgagt attgattcaa
     8641 atcaatttag atttgattcg caaaccggca aagaaaaacg gcatacccgt ttatacggat
     8701 atgccgtctg atgatgcagg ggactgctgt atttggcagt ggtttgtgtt ttagtcctct
     8761 tgcgccgctg tttgcaggta gttcggcaaa ccaattttgc cgattagttc ctgctgggtt
     8821 tcgagccagt cgatgtgttc ttcgttggtg tctttttgtt tttccaacaa atcacggctg
     8881 acataatcct gttgcgcttc tgctgtggcg atggcggcaa gcagggcttc gtgtttttcc
     8941 tgttctttgg tcaaatcgca ggcaatgatt tcttcggtgg actcgccaat cagaagcttg
     9001 cccagttctt gcaggttcgg caggccttcg aggaacagaa tgcgctcgat caaatcgtca
     9061 gcagctttca tttcaacgat ggactgtttg aagaaatgtt cgcccagttc ttcaaagccc
     9121 cagtttttca aaatacgggc gtgaaggaaa tattggttga tggttaccag cagcaagcct
     9181 aagtttttgt ttagctcgcg gataaccaga cggtcgcctt tcatacggac tccttttatt
     9241 ccgaatacgg attacagttg gctttggtag tagttgcctt cgccaattaa ctcgatcagg
     9301 cgaagctgct gttccagcca gtgggcgtgg tcttcttcgg tatctttcag ttgggcaacc
     9361 atcaggtcgc gcgtaacata gtcttgagcc tcttcgcaca gtttgatgcc ttttttcaac
     9421 gcatcacgta cttcatattc ggtttgcagg tcggctttga ggcaggaaac cacgtccgtg
     9481 ccgatattca gttcggcgcg tgccattttc ggcgtgccgc ccagcatcag gatgcggcgg
     9541 atgaagtctt cggcgtgtgt ggtttcttct tccatctcgt gattgagacg ttcaaaaagt
     9601 ttggtgtagc cccattcgga gtagaggcgg gagtggataa agtattggtc gcgtgccgcc
     9661 agctcgccag acagcaattc gttcatataa tcaacaacag cttgattgcc ttgcataata
     9721 tctctctttc tgtaacttgg ttttcggtat gtctgaacag catctgtgtc gctattcggg
     9781 atgcgtgcat tgtatgcaaa agctgtcggc atgacaaatt ttctatttaa aatacaaaca
     9841 attatcaaaa gaaatagggc gtattccccg atgccttcca aatcagtatg ctgtttctta
     9901 tcggtttttt gtgctgttaa aactaagaat ccatatcatc cataaagaac atttacactt
     9961 gttaaccata gcgaaaaaca aatagagcac ggttttttat caaaatttat aatgaatcgt
    10021 tctcattaac cgacagattc ttttgagatt atcggatttt gggaataaat tatgccgtct
    10081 gaggggcttt cagacggcat agacaattaa agctgattaa acaacgcggc agacagcagc
    10141 gtaggaaatt attttttact tcggaacggt attttgggtt taatgatgcc cgcaaccgcc
    10201 gtggctttcg cgtaaggctt cggcagcctg ttcgtcggcg tggtagctgg aacgtaccat
    10261 cgcgccgatg gcggcattgc tgaagcccag ttcgtatgct tctttttcaa agattttgaa
    10321 ctgctcgggc gtaacgtagc gcaagacagg caagtggccg tctgaaggct ggagatactg
    10381 tccgatggta atcatctcga tattgtgcgc ccgcatatcg cgcataattt cgcgcacgtc
    10441 ttcgtctgtt tcgcccaagc cgaccatgat gccggatttg gtcgggatgt gcggcatcat
    10501 ttctttataa cgttttaata agtctaaaga atgttgataa ttggcaccgg gacgggcttt
    10561 tttgtacagg ctgggatggg tttccaagtt gtggttcatc acgtcgggcg gagtttcggc
    10621 aaggattttc agtgcgatgt ccaagcggcc tcggaagtcg gggacgagga tttcgatttt
    10681 ggtgttcgga ctagtctcgc ggatggcttt gatgcagtcg gcgaaatgct gtgcgccgcc
    10741 gtcgcgcagg tcgtcgcggt cgacggaggt aatgacgaca taacgcaggt tcatggcttt
    10801 gacggattcg gcgaggtttt tcggttcgtc ggggtcgagc atattcgggc ggccgtgtcc
    10861 cacgtcgcag aacgggcagc ggcgggtgca gatgtcgccc ataatcatga aggtcgccgt
    10921 gcctttgctg aagcattcgc cgatgttggg gcaggaagct tcctcgcaaa cggtgtgcat
    10981 tttttgttcg cgcaaaatgt ctttgatttc aaagaatttg cgcgatggaa gtttggcgcg
    11041 tatccattcg ggctttttta atttttcctg aagggggacg actttgatgg ggatgcgggc
    11101 ggttttgtcc gcgcctctga gtttgatgcc gcgtttgggg tcgtcggttt tgatttcact
    11161 cattgttgtc tgctttcggt gtgaattgtg tttcaaggtg tgcggtgagt ttggcggcga
    11221 cttcgtccgg cgtggggcag ggttggacaa aatccgcgat ttgcgtcatt tccataccgg
    11281 cgtagccgca ggggttgatg tgggtaaacg ggcttaaatc catattgacg ttgagcgcaa
    11341 gcccgtgata gacggagccg tttttgatac gcagccccag tgaggcgatt ttgcgttctc
    11401 cgacataaac gccggggcgt ttggggtctg ccgccgcttc gatgccgtat tctgccaatg
    11461 tggcgatgat gctgttttca agcgcggaaa cgatgtttct aacactggtt ttgcgccgtt
    11521 tgaaatcaat catcgtataa acgaccaatt gcccgggccc gtgataggta atctgcccgc
    11581 cccggtcgat ttggacgacg ggaatgtcgt cgcgaatcag caggtgttcg ggttttcccg
    11641 ccagtccttg tgtgaacacg ggcgggtgtt cgacgaccca cagttcgtct tcggtgtcgg
    11701 catcccgtcc ggcattaaag gttttcatcg cttcaaaagt cggcagatat tcgaccaaac
    11761 ctttgtgtat gattttcatc tcaaagtacc actttgacca gttcgtgcga agtcagcgca
    11821 cggtagatgt tgtccaattg ttcttggttt tcaaccttta cctgtacggt ggcgccagta
    11881 tagttgcctt tgctgctcgg acgcgtggtg atgtggtgcg cctgcgtgtc gggggcgtga
    11941 aggcggacgg tgtccaatac cgcctgctcg aactcgggat gcaccgcgcc cattactttc
    12001 aatgggaagg tgcagggaaa ttcgatgagg gatgttttgt tttcttgttc ggtcatgatg
    12061 tgctgccttg tcgtgtacgg tatgccgtct gaaggcgggt ttgcctttca gacggcatcg
    12121 gatgtgcgtt attttagcct aaaccgcgat aacaggctat cgggaaggcg gaggcttttt
    12181 tgacggcgcg gcggttctgc tatactggcg cacaatatta tttttagaag ggtggttttt
    12241 atgtatcgga ggaaagggcg gggcatcaag ccgtggatgg gtgccggtgc ggcgtttgcc
    12301 gccttggtct ggctggtttt cgcgctcggc gatactttga ctccgtttgc ggttgcggcg
    12361 gtgctggcgt atgtattgga ccctttggtc gaatggttgc agaaaaaggg tttgaaccgt
    12421 gcatccgctt cgatgtctgt gatggtgttt tccttgattt tgttgtttgc attattgttg
    12481 attattgtcc ctatgctggt cgggcagttc aacaatttgg catcgcgcct gccccaatta
    12541 atcggtttta tgcagaacac gctgctgccg tggttgaaaa atacaatcgg cggatatgtg
    12601 gaaatcgatc aggcatctat tattgcgtgg cttcaggcgc atacgggcga gttgagcaac
    12661 gcgcttaagg cgtggtttcc cgttttgatg aggcagggcg gcaatattgt cagcagtatc
    12721 ggcaacctgc tgctgcttcc cctgctgctt tactatttcc tgttggattg gcagcggtgg
    12781 tcgtgcggca ttgccaaact ggttccgagg cgttttgccg gtgcttatac gcgcattaca
    12841 ggcaatttga acgaggtatt gggcgaattt ttgcgcgggc agcttctggt gatgctgatt
    12901 atgggtttgg tttacggctt ggggttggtg ctggtcgggc tggattcggg gtttgcaatc
    12961 ggtatggttg ccggcatttt ggtttttgtt ccctatttgg gtgcgtttac gggattgctg
    13021 ctggcaaccg tcgccgcctt gctccagttc ggctcgtgga acggcatcct atcggtttgg
    13081 gcggtttttg ccgtaggaca gtttctcgaa agttttttca ttacgccgaa aatcgtggga
    13141 gaccgtatcg ggctgtcgcc gttttgggtt atcttttcgc tgatggcgtt cgggcagctg
    13201 atgggctttg tcggaatgtt ggcaggattg cctttggccg ccgtaacctt ggtcttgctt
    13261 cgcgagggcg tgcagaaata ttttgccggc agtttttacc ggggcaggta ggcggttccg
    13321 aaacatattt gaagcggaat acaaccctgc ccgagtttaa ataaaaatgc cgtctgaacc
    13381 ccgaaaacag agcttcagac ggcatttcat cacggcttat ttggcggttt tgctgctgtc
    13441 gataattttc ataccggcag aaatcaggct gccgatgtcg gcaacattgg cgggcataat
    13501 cagcgtattg ctttctttgg caagattgtt gaacgcggcg acgtattgtt ccgcaatctt
    13561 caggttgacc gcatccgcgc cgccttgggt ttgaagggcg gcggcaattt gacggatggc
    13621 ttcggcattg gcttcggcaa caaggcgcag ggattccgct tcgcctttgg cgcggttgat
    13681 gcgggcgatt ttctcggcat ttgacgcatt gaccgcggcc tgggcctcgc cttcggattg
    13741 ttggatttcg gcttcgcgct gaccactggc aaggttgatt tgttcgattt tacggccttc
    13801 ggattcggcg atacgggcgc gtttttcgcg ttcggcagta atttgcgcct gcattgagcg
    13861 aaggatttct tgcggcggaa ccaaatcctt gatttcgtaa cggagaactt tcacacccca
    13921 agctccggcg gcttcatcga gggcggaaac gacggtgctg ttgatttcgt cgcgttcttc
    13981 aaacgttttg tccaactcca tacgcccgat aacggaacgc agcgtcgttt gggcaagctg
    14041 ggtaatcgcc ataatgtagt tgctcgaacc gtatgaggcg agtttgggat cggttacttg
    14101 gaaatagatg atgccgtcaa ccgtcagctg ggtgttgtcg cgcgtgatgc agacctggct
    14161 gggtacgtct aaagggattt ctttcagcga atgacggtag gcgacgcggt cgataaaggg
    14221 aatcaaaata ttcaaaccgg ccgtcagggc gcgatggaaa cgccccagcc tttcgacaac
    14281 gtggacttcc tgctgtggga tgacgacaaa ggatttgaag ccgaaaacgg caacggctgc
    14341 caacaagata atgaaaaatt ccataattcc tccgagtgtt aaggttttgc gatgataaga
    14401 aggttgcctt ccttgcggac gattagggcg cgcgttcccg gttcaaacac atcctgcccc
    14461 gtattttgcg cctgccagtg cgtaccgcga taaaaaactt cgtaacggtt gccgcctgcg
    14521 tgccggagga tttcggcata ttgcccggca tccaaatcct gatatgaatc cgtttcaact
    14581 tttcccacgg cggttttggc gtgtacgaac caaataccca gcgcggaaag cagagcggcg
    14641 gtcaagacgg cggcaggcgt gctgccggtc agcccgtaag caatgcccga acccgccaaa
    14701 gctgcactga caaccagaag ataaaccgtt cccgtcaata attcgatgat taagacggca
    14761 acagcagcaa caaaccatac agtcatacat ttccccacaa agcgcgtcgt ttgacaaaat
    14821 cacgcgatat cagcagtata gccgaatttg aaaggatagg gcagatatgg atacttggca
    14881 cgatgcactg ggcaacgaaa aacggcaacc gtattttcag gaaattttaa atgcagtcag
    14941 gcaggaacgt ttgtcggggc aaatcatcta tccgccggcg gaggatgtgt tcaacgcatt
    15001 ccgcctgaca gcgtttgacc gagtcaaagt cgtcattctc ggacaagacc cgtatcacgg
    15061 ggcagggcag gcgcacggtt tggcattttc cgtccggcag ggtatccgca taccgccgtc
    15121 tttactcaat atctacaaag aattggaaac cgacatcaac ggtttttcca ttcccgatca
    15181 cggctgcctg acggcttggg cggagcaggg cgtattgctt ctgaacacgg ttttgacggt
    15241 gcgtgcagga caggcgcatt cgcacgccct tttaggctgg gaacgcttta ccgataccgt
    15301 catcaggcag cttgcggcgc accgcaagca ccttgtcttc atgttgtggg gtgggtatgc
    15361 gcaacaaaaa aggaaactga tagacagtca aaatcatttg atactgaccg caccgcatcc
    15421 gtctcctctg tcggcatatc gcggtttttt cggctgccgc catttttcac aggcaaacag
    15481 ctatttgagc cggcacggta tcgatccgat aaactggaag ctgtgaatgc cgatatagcc
    15541 gttgccgccg gcgtgttaaa atcgcgtttg atttgtaatt tccatttatt aggcaaaacc
    15601 ttatggcaat caaaaaaaaa tcagctttat gcatcacttt gggcgggctg cgacgagttg
    15661 cgcggcggta tggatgcgag cctttataaa gaactatgtg cttacgctat tgtttttaaa
    15721 atatgtttct gataagcata agtacggcgg cggcatgatt gagctgcacg ccgatacgac
    15781 ttttgacgac atcgtcaaac tcaaaaacac cgccgacatc ggcgaccgcc tgaataagat
    15841 catcgcccaa attgccgaag ccaacgactt aaaaggcgtg atcgacgttg ccgacttcaa
    15901 cgacgaagac aaactgggta aaggtaagga gatgatcgac cgtttgagca ggcttgtcgg
    15961 catttttgaa aagctcaacc tttcttccaa ccaagccgaa gacgacgatt tgttaggtga
    16021 tgcctacgaa tacctgatgc gccattttgc gaccgagtca ggcaaatcca aagggcagtt
    16081 ttacacgcct gccgaagtct cgcgcattat ggcgaagatt atcggaatca gcgcagattg
    16141 cccgtccagc accagcgttt atgacccgac ctgcggctcg ggttcgctgt tgctcaaagc
    16201 cgccgcccaa gccggcagcc aaatcagcct ttacggtcag gaaaaagatg tggcaaccgc
    16261 gtcccttgcc cgtatgaata tgattttgca caacaacgaa accgccgaaa tcaacaccgg
    16321 gaacactttg tccgattcgt ctttccgtga tgaaaacgac gggcttaaga ccttcgattt
    16381 tgccgttgcc aattcgcctt atcccgcccg aaaaaaacgg cgattacgcc tttttgctgc
    16441 atctgctcaa aagcctgaaa ccaagcggca aaggtgcgat tattcttccg cacggtgtgc
    16501 tgtttcgcgg caatgccgaa gcgcgtattc gcacggaatt gcttaacctt gaccttatta
    16561 aaggcattat cgggctgcct gccaacctgt tttacggcac gggcattcct gcctgcatca
    16621 tcgtcatcga caaagaacac gcccaaaccg cccaatttgc cgaagaggga acaaaccaag
    16681 ttatcagtgg cggcattaaa gacggcaaca aaaaccgtct gcgtgagcaa gacattcaca
    16741 aaatcatcga cactttcaca aacctcgtta cagccgtatg gtgcatttaa gcgaaatcgc
    16801 agcacaagat tacaacctta atctgcctcg ctatatcgac agcggagaag ttgaagacct
    16861 gcaaaatctc gccgcctatc tttatggcgg catacctgcg cacgatatgg acgcattgga
    16921 agcctattgg caagttttag gccgtatgaa aaacgagttg tttgccgaac acgatggccg
    16981 ctttaccact atacaacgga atcgattgca aatctttccc actctcaaca gcttaaaatc
    17041 ctgcgggatt ggtgtggaat ttagggctaa tctagtacag ccccaaattt aatccactat
    17101 aaaatcgaaa gcagccaaat caaagcccat atattggcgc accccgatta cgccgccttc
    17161 aaagccggac acctggcaaa gtttgccgcg tggcacactc aaaacgacct tgccgccatc
    17221 caaccgggca ggcttatccg gaaatggagc gaaagcctgc tggacgcgtt caaacccggc
    17281 agcctgattg aagaatacga tttctaccaa atcctgacgg actactgggc ggaaaccctg
    17341 caagacgatg tttatctcat cgcccaagat ggctggaagg cggttaaaaa cctggccgaa
    17401 atcaccaaag aaagcgatga agacgcgaac ctgaccgtcg tctttgagga aaccgaaacc
    17461 ggcaaaaaag gcaaagccaa aaccaagcgc atcagcaaaa aataccgcag cgaagtcatc
    17521 gcccccgagc tggttgcccg ccgctacttt tcagacggca tcgccaagct ggaagaaaaa
    17581 caaagcgagc tggaacgcct aagccaagaa ttggaaaacc acatagaaga acacggcggc
    17641 gaagagggtg cgctgaacga cgtattggat gcaaaaggca aactttccgc caaacttctg
    17701 aaaaccgcat tggaagaaag cggcatagaa gaaggcgaac gggctgtttt acaaaccacc
    17761 caaacactga tgacgcagga aaaagccgcg aaagacgcag tcaaaaccca aatcgaagcc
    17821 ctgaaccttg ccgtattcaa acaattcggc cgactttccg aagccgaaat caagcagctt
    17881 gccgttcaag acaaatggct tgccgattta caaagccgaa tcgaaaatcg cttggaaaac
    17941 agtattcagc agcttatcag ccgcttgaac acgctggaag accgctaccg cagcccgatg
    18001 gccgagcttg cccgagaagt ggaaaagtgg caaagcaaag tcaatgccca ccttgaaaat
    18061 atgggttttg gaggctgaaa tggcggcaca gacaggctat aaggcgagcg ggttttgaga
    18121 cctttgcaaa attccccaaa atcccctaaa ttcccaccaa gacatttagg ggatcgcggt
    18181 tcgggtgtcc gcaccgctta atacgtcgtc gtccacgaac tgacccattt gctcgaacgc
    18241 catcgcaacg cccgttttat ggcgcatatg gacaactttc tcccaaactg gcaaagcatc
    18301 aaacaacagc ttaatgcctt ggagttattt gcacaaatat ataatttaac ataatatata
    18361 ttatgcgaac tatcggaaac agttgtacgt gtccctgtgg cctttccaag taggtaaagt
    18421 atggacaatg cggctgaatg tatagcccgg agcatccgcg tatccgaagg tactgaacga
    18481 tatgccctgc atccctttcg acgcttccat ccagccggta ttgggaaata cgttgacctt
    18541 caatacgata tacaggcagc cgacacacag gatatgccgc tgctttttca tcatttcttc
    18601 tgtcaaatcc ttggaacagt tgatttgaaa acacggcttt acatacgtcc caattctctt
    18661 tgcagtgctt ggatattttg ctgcctgtcc aatacattgc tttgcaatgc gttgatttct
    18721 tgatggttga tgttgccgcc ttttgccaga cgtgcttgtg ataacatttt ttgtgcttca
    18781 accaatgctt tgcgttcgtt gctcaattct gtttcgagaa tggagcgtct gctgttgttt
    18841 gagggtgctt gttgcggcgg cggcgtattg gattttgccg gcttggatac tgttttgacc
    18901 ggggctttat atttgacagc ctgtccgccg tttgacggtg atggtgtcgg ttcggatgtt
    18961 tggggcggga tatagcgttc gctgctgtag ttgccgattg ggggcaaatc ggttgagagg
    19021 cagcttttgg acggcttggt ggtgtaaacg gtttctccgt tgattgtgca ggtgtagatt
    19081 ttggccgcat tcgcacccaa tgggcttgaa atcagggaaa agttgattag gattaagggg
    19141 agttttgatt tcataatgtg cttgatgctc ggataattat ttgatttttt ggtatttttt
    19201 gtaatgcttt cttggcgttt tacgggcttt tcttttcggt tggttgcttt tgattttctt
    19261 tcggggcttt ggcagcctgc ttgttctttt tcccggcagg ttttttgttt ttctttttac
    19321 tttctatgtg tattttggct tcttaactga gttttttaat tttcaggcgg tatccgcctc
    19381 cctgatggct gctgatttta ggtaaatccg catcggcgca cgatcctgct ggggctgatg
    19441 tatatacgct tttgccattt gagttgcaat ggtatacgga ggcttgcgcc gccgcgccgg
    19501 aaagggacag gagacccaag gcggcaaaaa gtctgatgtt tataccggta aaaggcggtg
    19561 ataacgcccg aattataccg ttattggcag gcaaagataa gcaccctgtc cgcgcttctt
    19621 tatcgctcgg caaactgttt ctcgatttca gtttcaatcc gttccaattc ttcttgacaa
    19681 gccagccaaa cctcttcgat ttgggcaagt tgtgttttga cttttgtcag cagggataag
    19741 gtattttgca atttttcttt gtttgcttca aggtaagcgt cttcttgtgc taaaaatgct
    19801 tcacatgccg tctgaatttc ggaaagctgc gccatttctt tttcggcacg gtctattttc
    19861 tgctgtatcg gcttgccgcg tcgggctttt tcctgacgga tttgcgcttc gatgcgcttg
    19921 gtgtctttgc ggctttggct ttgtgcggat gctgcgggtg cggcggcggc gttttcctgt
    19981 gccaaacgcc attggcggta atcgttcaaa tcgccgtcga agttcttcag acggcctttg
    20041 tcgatcagga ggaagctgtc ggtagtcgct tcaagcaggc tgcggtcgtg cgatacggcg
    20101 attaatgcgc cttggaaact ttgcaacgcg agcgttaagg cgtggcgcat atccaagtcc
    20161 aaatggttgg tcggttcgtc aagcagcaac aggttcggct tttgccagat aatcatggca
    20221 agggcaaggc gggctttttc tccgccggaa aaaggttcgg ttttctgtaa cgccatatcg
    20281 ccaacaaaat tgaagccgcc gaggaaattt cggatttctt gttcgcgtac ttcgggagaa
    20341 agctgctgaa tatgccaaac agggttttgg tcggcgcgga tggtatcgag ttggtgttgg
    20401 gcaaaatagc cgatattgag tttttcggaa cggacgatgc tgccggagag caaatcgatt
    20461 ttgcccgcca aggctttgat aaatgtagat ttaccgctgc cgttgacacc caataaacca
    20521 tagcgcgcgc cgctttccag cgacagggta atgtcgtgca aaacagtttt gccttcgtaa
    20581 cccaaatctg cgtgttccag cttcagcaaa ggattgggca gatgctcggg attgtaaaac
    20641 tcaaaggaaa actcgctgtc caaatgcgcg ggagcgatgc gttcgagctt tgccaaagcc
    20701 ttcatgcggc tttgcgcctg aacggctttg gtggctttgg ctttgaagcg gtcgataaag
    20761 gattgcagat gtttgatttg cgcctgctgt ttgacatagg cggcttgttg ctgcgcgaga
    20821 cgctgcgcgc gttcgttttg gtaaaaatcg taattgccgc cgtattgcgt gagcttttgc
    20881 tgggacaatt cgatggtttg ggtggttgcc gcattgagga aatcgcggtc gtgggaaatg
    20941 atgatttgcg tgcagggtaa agaggcaagg tggttttcca gccacaagac ggtttctaaa
    21001 tccaagtggt tggtcggttc gtcaagcaag agcaaatcgg cgcggcaaat cagggcttgc
    21061 gcaagattca ggcgcatacg ccagccgccg gaaaaggatt tgacgggacg gctgtgttct
    21121 tcttgcgaaa aacccagccc gttcaacaat ttcgctgcac gggccggcgc ggtataagcg
    21181 tcgatttctt ccaatttggc gtgatattcc gcctgtttca taccgtcgtt ttgcgcttcc
    21241 gcctgcctca atgccgtctg aagggtctgt aactcggcat cgccctgcaa aacgtaatct
    21301 aaagcggaaa tatccaaatc gggcgtttct tgggaaacgg aagcgagccg ccagtttttc
    21361 ggaatcgaga catcgccgcc gtcctgagtg atttcaccct tgattaaggc aaacaggctc
    21421 gatttgcccg tcccgttttt gccgatcaaa ccgacgcgct gaccgggatt gacggtagcg
    21481 ttggctttgt cgagcaggac tttcaaaccg cgttgcaggg tgaggttttt gatttcaatc
    21541 ataacggaaa catcgtcggg cgggaaaagc ccgtatttta cctgaaaatc agtgccgatg
    21601 ccgtctgaaa tgggaaattt acggctgaag ccaagcccaa gccctgcgcc cttccgagtg
    21661 caggaaaacc aatgtcctga atgccgaatc ggtattcatg cattccacgc tgattccgat
    21721 tcgggaaaaa tccgccatga ttttgggatg gataaactcc tgagccgcgc ccgtcccgat
    21781 aatcaatatt tccggatagt caacaggttt gatgtcggac aacaggtttt ccggagtcag
    21841 atcggacaag gttcggcatt gcgacaggca gaccgaatcc ttatgtacaa gcacgggttt
    21901 atggaaactt tgacccgcca atcggatttc acccgaccgg tggccatact ctgtaaaatg
    21961 tccgtctatc ggattttctt caaacaacat ttttttaccc cgtcgccgca tcatctacac
    22021 cgaaaggggt gcaaaatcag gcgaattcat gtaggattgg cagatttcat ctgacccgcc
    22081 tgccgatttc agacggcatt tgattcaaag tgcggcacaa ttatatcggc agcggatatt
    22141 ttcgtctttc aatatttaca tttcagtcgg cttacaagga gacacaatga agccagtaaa
    22201 catcggtctt ttaggtttgg gtacggtcgg cggcggtacg gctgccgtgt tgcgggacaa
    22261 cgcggaggaa atttcccgtc gcttggggcg cgaaatccgt atttctgccg tgtgcgattt
    22321 gagtgaagaa aaagcccgac aaacctgccc gtccgcagcc tttgtcaaag atccgttcga
    22381 actggtcgca cgtgaagacg tcgatgtcgt cgtcgaattg ttcggcggta ccggcattgc
    22441 caaagatgcg gtgttgaaag ccattgaaaa cggcaaacac atcgttaccg ccaacaaaaa
    22501 actgctcgcc gaatacggca acgaaatctt cccgctggcg gaaaaacaaa acgtcatcgt
    22561 ccaatttgaa gcggcagtag cgggcggtat cccaatcatc aaagccctgc gcgaaggttt
    22621 ggcggcaaac aggattaaat ccatcgccgg cattattaac ggcaccagca acttcatcct
    22681 ctccgaaatg cgcgaaaaag gcagcgcgtt tgccgatgta ctgaaagaag cgcaggcatt
    22741 gggttatgcc gaagccgatc cgaccttcga catcgaaggc aacgatgcgg gccataaaat
    22801 caccatcatg agcgcactgg cattcggcac gccgatgaac ttttccgcct gctacctcga
    22861 aggcatcagc aaactcgaca gccgcgacat caaatacgcc gaagaacttg gctatcgcat
    22921 caaactgttg ggcattaccc gcaaaaccgg caaaggcatc gagctgcgcg tccaccctac
    22981 cctgattccc gaaagccgcc tcttggcaaa cgtcaacggc gtgatgaacg ccgtgcgcgt
    23041 caacgccgat atggttggcg aaaccttata ttacggcgcg ggcgcgggcg cattgccgac
    23101 cgcttccgcc gtggttgccg atatcatcga catcgcccgc ctggttgaag ccgataccgc
    23161 ccaccgcgta ccgcatctgg cgttccaacc cgcgcaagtc caagcgcaaa ccatcctgcc
    23221 tatggacgaa attaccagca gctactacct gcgcgtccaa gccaaagacg aaccgggcac
    23281 gctggggcaa atcgccgcgc tgttggcaca agaaaacgtg tccatcgaag cactgattca
    23341 aaaaggcgtg attgatcaga ccactgccga aatcgtgatt ctgacccaca gcacggtcga
    23401 aaaacacatc aagtcggcaa tcgcagccat cgaagcactg gattgcgtgg aaaaaccgat
    23461 taccatgatc cgcatggaaa gcctgcatga ctgagccgaa acacgaaatg ccgacggaag
    23521 agcaggttgc cgcgcgcaaa aaagcaaaag ccaaaatccg caccatccgc atttgggcgt
    23581 gggtcatttt ggcgttgctc gctttaaccg ccctgctctc ccaatgcgcg atgtccaaac
    23641 cgcaggcaaa acagaaaatt gtcgagtctt gcgtgaagaa tattccgttt gccgaaaaat
    23701 ggcaaaacga tttgcgggcc cgcggtttag attcaaacaa tacccgcctc gccgtcgact
    23761 actgcaaatg tatgtgggag cagcctttgg acagattgag cgagaaacag attagatcct
    23821 tcggcaaact cggcgcacaa gaacagcttg acctgctcgg cggcgcaaat gcctttgaag
    23881 cacgtgacaa gcagtgtgtt gccgatttga aatcagaata atgtggaccg ataaaaaagc
    23941 cgattcttta aagaatcggc ttttttcata aaaaacggct tacagtgcgt ctttcaatgc
    24001 tttacctgca cggaatttag gcgttttggc ggcggcaatg gtcagaggct cgccggtttt
    24061 ggggttgcgg ccttggcgtt ccgcacgttc gcccacgtag aaagtaccga aaccgaccaa
    24121 agtaacggtg tcgccttgtt tcagggcggt ggttactgca ttggtagtgg catccaaagc
    24181 tttttgtgcg gcggctttgg aaatgtcggc ttcttgagca atcgcttcga tcaattcaga
    24241 cttgttcaca atcagtccct tcctgtctta aaaaatgatg aaatgcccga atactcgggg
    24301 tttgtactgc ttgagcaact ttcgctttat agcaattctg aaattgccgt gtcaagcaaa
    24361 aaatacggaa tcaccctatt tgacaggctt tcaggacgaa accgcatttt tacaacacat
    24421 ttcctgcgtt tcaatgtttg gttgccctgc tgcggggttt tggttttgaa gcggattccg
    24481 ccgccgcttc cgcaccagaa ggttctgccc aaggctcagg ctggctttcc aaacccagag
    24541 ccaatacctc gtctatccat ttgaccggat ggatggtcag gccggttttc acgttttcag
    24601 ggatttcttc caagtctttg aggttgtctt ttggaattaa aacgtgtttg atgccgccgc
    24661 gaagcgcagc caacagtttt tccttcagac cgccaatcgg caggacttcg ccgcgcaggg
    24721 taatttcgcc cgtcatggca acatcggcgc gcaccgggat tttggtgaat gcagacacca
    24781 tcgccaaagt catggcaatg cccgcactcg gaccgtcttt cggcgtcgca ccttcgggaa
    24841 catggacatg gatgtctttt ttctcgtaaa aatcaggagc caagcccact gattccgcgc
    24901 gggagcggac aaccgaccac gctgcggaca cggattcctt catcacatcg cccaactggc
    24961 cggtgcactg aatcacgccc ttacccggca atgctgcggc ttcgacggtc agcaattcgc
    25021 cgccgacttc cgtccacgcc aaaccggtta cttgtccgat acggttttcg ctttcggcaa
    25081 cgccgtaatc gaagcggcgc acacccaaat agtcgtgcag atttttctca tttactttaa
    25141 ccgccctggg cttggctttt ccggtttttt tggtttttga gtcttcgtcc aaggtaatct
    25201 gcatcaccac cttgcggcag attttggcaa tctcgcggtc gagcgaacgc acgcccgcct
    25261 cgcgggtgta gtaacggata atatcgcgca ccgcgctttc ttcgattgcc aattcccctt
    25321 cttttacgcc gttgcgcttc atttgcttcg gcacgaggta ctgcatcgcg atattgattt
    25381 tttcgtcttc ggtatagccg gacagacgga tgatttccat acggtcgagc agcggtgtcg
    25441 ggatattcag gctgtttgaa gtcgcaataa acatcacatc gctcaaatcg taatccactt
    25501 cggcataatg atcggcgaac ttgttgtttt gctcgggatc aagcacttcg agcaacgcgc
    25561 tggcgggatc gccccggaag tcgctaccca atttgtcgat ttcgtcgagc aggaacaatg
    25621 ggttttttac gccggctttt gccatattct gcaaaatctt accgggcatg gagccgatat
    25681 aggtgcggcg gtgtccccgg atttcgcttt cgtcgcgcac gccgcccaaa gccatgcgga
    25741 catatttccg ccccgttgct ttggcgatgg attcgcccaa agaggttttg cccacgcctg
    25801 gagggccgac caggcacaga atcgggcctt tgagtttgtc catacgtttt tggacggcga
    25861 ggtattccaa aatccgttct ttgacttttt ccaggccgta gtggtcggta tccagcacca
    25921 atccggcttt ggcgatgtct ttgctgacgc gggatttttt cttccacggc agttcgagca
    25981 gggtgtcgat gtagttgcgt acgacggtgg attcggcaga catcggcggc atcattttga
    26041 gttttttcag ttcggacagg catttttctt ccgcttcttt gctcatcccc gcctttttga
    26101 tgcctgcttc caaggcatcc agttcgccgt tttcgtcttc ttcgcccaat tctttgtgta
    26161 tcgctttaat ctgttcgttc agataatatt cgcgctggga tttttccatt tggcgtttga
    26221 cgcgtccgcg tatgcgtttt tcggcctgca taatgtcgag ttcggattcc agctgtgcca
    26281 gcaggaattc catccgtttg ccgatttcgg gaatttccaa aatctgttgg cgttgcgcca
    26341 gtttcaactg caaatgcgct gcgaccgtat cggttagccg gctgttttcg gcaatgccgt
    26401 taatgctgcc gataatttcg gcggggattt ttttattgag tttggcgtat tgttcaaact
    26461 gcgccaacag ggtgcggcgc acggcttcga ggtcggtatt gccgcccgtg tcttcttcca
    26521 cgaccgtctc tatatgggaa acgaacagac cgcccgtgtc ttcaatggtc agaacacgtc
    26581 cgcgatacag cccttcgacc aatactttta ccgtgccgtc gggcagtttc aacacttgca
    26641 ggacttgtgc gaccgtaccg gtctgataca ggtcggcggc aaccggttct tctaccgccg
    26701 catcggtttg cgccaacagg aaaaccggct cctcgcgggt aatggcgttt tccagtgcgg
    26761 cgatggattt cggcctgccg acaaacagcg gcagaaccat atgcgggtaa acgacgacat
    26821 cccgcaaagg aagggttgcc aaggcggcat attcctcaaa atgcttttct ttttgtgtca
    26881 taggtactct cttgtgtctg acagattgcc gattttcgcg tacattgggg ttgaaggtat
    26941 tatttcaagc atatgtggtt tatttatgga gtttgatgcg atgccgtctg aaacattccg
    27001 gcttcagacg gcatgggctt ggaaagacaa ggcgggaaca aaaaactgtt ctgtgttgcc
    27061 gctccttgct gtaccatccg tatggtttgc ggttctgccg ccctattttc aaaacatgac
    27121 gcaggtattc catgtcttct tttatttttc ccgataacgg gttcgaatgg cgcaacgaaa
    27181 gtctgcaaaa gccgcccaaa ggttggcatt atgtccgcga aagtggtgca aacggcattt
    27241 tgaaggctgc ctatcaaaat attgcaactg tctggcaggg cgatttccac aatgccaaac
    27301 aggtgctttc cgcaatgaag aagaaggtcc gcaaacctgc cgccgtccgt tccgatgcgg
    27361 atattgccgc ccttttccac gcccaccgta tgaagcaggc gcagcagagc cgtattctga
    27421 atatgcttgc cgttgaaatc cgccccggtt ttgtgttgga caacaaacgc gcgcccgata
    27481 tacgcgccgc cctgctcgac gtgtacggag aggcggacgg caaaccgttt ttcctgccgc
    27541 tcaatctgct gctggggttt atgggtgcgc acgagtggca taagaaaggg gttgccgttc
    27601 cgcagctggg cggcagcata cacgttcctt tcggcgtatt ctcgccgttg cgcggcgaat
    27661 acctcgacct gctcgcccat gcgccgtcaa cgggttttca gacggcattc gatatcggga
    27721 cgggctcggg cgtgcttgcc gccattttgg cgaaacaggg cattccttcc gtcatcggca
    27781 cggataccaa tccgaaagcc gtcgcctgcg cccgtgccaa tattgcccgt ttgggctttg
    27841 aaaaacaggt tgagatacgg gaaaccgatc tgtttcccga agggtttgcc gatctgattg
    27901 tctgcaatcc gccctggctt cccgccaagc cgacttccgc cgtcgaatcc gcgttatacg
    27961 accccgaatc tgcgatgctg gctgcgtttt tgcgggatgc gccgaaacat ctgaatcccg
    28021 acggagaaat ccgcctgatt atttccgacc ttgcagagca tttggggctg cgccctgccg
    28081 attttttgga aaaggcattt atccgggcgg gtttgcgtgt tgccgatatg atgaaaacca
    28141 agccgaagca caaaaaagcc gcgaatccga gcgatccgct tgcttttgcg cgaacccggg
    28201 aaaccacttt cctataccgt ttgaaaaagg cataaggggc ggcggcgcgg cattcgggcg
    28261 gattattctt gtgaaaatac ccgctcgagc atactgccca atgccgtctg acgcgttttg
    28321 acagtggcgg cagcttgccg gaaggtttct tctccgccga agaatacgaa cttttccctc
    28381 gcgcgggtaa tggctgtgta aagcagccct ctgctcaagc ccgaaagcgc gtcgtcccct
    28441 tcgtccgaag gtgcggcgga aggcggcagc agccagactt cccgatattc cgaaccttgg
    28501 cttttgtgga cggtcatggc gaatgcgggt tcgaaatcgg gcaggcagct taccgctacc
    28561 tttttaaatc cgtccgcatc ggcaaaatag gcggcaaggc cgccctgccg tccgacatct
    28621 tccataatca atccgatgtc gccgttgaac agttcaagcg cgtagtcgtt ctgtctgatc
    28681 ataatcggtt ctccggcgaa ataggcctga tgttccggta tgttcatttt gcggcgtaca
    28741 tggctgcaat aggcttcgtt gaagtcttcc gcatcctgcc gccacgccgc gaggacgacg
    28801 atatccgaga tgcctgcgta tgcggcttcg atattgccgt cttttaccgc ctgccaatag
    28861 gctttgtgcg cccggtacaa cctttcgact cgagcgttcg gactgcattc cgaatgttcc
    28921 agttcgtccg gaaaccggtc aaacaatgcc cacgccccct catcgcccga tacggcggca
    28981 cgggcaaggc agccgatgcc gctgttgtcg ccgaagcggt ggctgaacga cagatgggcg
    29041 gtgttttgcg ccaacaaggg cggatttgca ctgacgctga aaccgtgttc cggaaggaag
    29101 ccggccagcc tttggtgcgt ttctccgtcc aaaacggttt tttgcgacag gacggacagc
    29161 acgtccccga ttccgacgga cgggagctgg ttttcatcgc ccagcagaat cacgcgcgcg
    29221 ccggttttga ccgcttttaa aagttgcaac atcaataccg tatccagcat agaggcttca
    29281 tcgataacca atacgtcaaa cggcagcggg taaacagggt tgaacgccgc ctgcattttg
    29341 ggcgggcgca gcttcagcag tcggtggacg gtttgccctt ccagtttgag caaatggcgg
    29401 cggacggcct ccggcgcgtc aaaaccgttg attgcacggt gcagcgcgcg cgccatatgt
    29461 gccgccgctt tgcccgtcgg tgcggcaagc gcgatgttgg gaagattttc gtcttcaccg
    29521 caaatcagcg ccagcagttt ggcaaccgtt gtcgttttgc ccgttcccgg cccgccggta
    29581 atcaccataa aagactgcaa cagtgccaag gcggcggcat cgcgctgccc ttcgctgccc
    29641 gtgccttgaa accattttgc gaggttttgc ctcgcgcctg ccgcgtcggg ggcggacgtg
    29701 tcggctgccg ccaagcgttt tatctcggca gccaaatcgt attccaactg ccacatcctg
    29761 cccaaaaaca gccttctgcc ttccaaaatc aaaggcgcgg cggatgttcc gaccacgggt
    29821 gcgagtgccg acagcgcgtc agcctcgcca ccgctcaaac ggataaacga atgaccgttt
    29881 tgcaatgcct gaaacaggcg ttcggtgcag tttgcaagca cttcgtcgct ggaacccgca
    29941 tagcgtgcca aaaaccagat tgccgccctt gccgccgctt gggcaaattc atctgtctgc
    30001 cgttccatcg tattccttgt cgaaatgccg tctgaaggcg tggggcttca gacggcgcgg
    30061 tgttgtttcg gctgtttagg cgtttgcgcc ctgtttgggg tgcaggctgc cgtctttcat
    30121 gaccatcacg cgcccgaagc ggccggcgag ttcgtcgtcg tgcgttacga ccaccagccc
    30181 cgttcccaat tctgttttca gttccagcat catatccaaa acattccttg cgtttgctcg
    30241 gtcgagattg ccggtcggtt cgtcggcaag caggcatttg ggttgcgtaa ccaaggcgcg
    30301 tgcaatggcg gcacgctgcc gctcaccgcc tgaaagttcg cccgcgcggt gcgtcgaacg
    30361 gtgtttcagt ccgacctttt ccagcatcgc cattgccgtt tctgctgcct cttcacggct
    30421 ttttttgccg atcagaagcg gcatcatcac gttttccagt gccgaaaatt caggcagaag
    30481 atggtggaac tggtacacga aaccgagatg gcggttacgt aaatcgccca aacgccgctg
    30541 gtttaaggta cgcaaatcct cgcccatcag cagcaccctg ccttcagacg gcatatccag
    30601 cccgcctaga atatgcagca gcgtcgattt tccgctgccc gaagaaccga tgatgccggt
    30661 gctttccccc gcgcggattt ccaaatccaa gccgtgcagc accggaacat ccaaaccgcc
    30721 gtcgcggtag cgtttgccca cgctttcgca tttcaaaatc aactcactca taacgcaaag
    30781 cctccgccgg ttgggttttt gacgcgcgcc ggctcgggta gagcgtggca acgaaagaca
    30841 gtcccaaaga aatgcaggca atcagggcaa cgtcgcccat atcaacatcg ctgggcaggt
    30901 agtcgataaa ataaacctgc gaattgataa ggtggacacc gagcaggttt tcaaaaaatg
    30961 ccacgaccct gccgacgttc caacccaaaa gcacgccgca gaccacaccc gccagcgtgc
    31021 cgaaaaagcc tgaaaacgcg ccctgcacca taaaaatctt catcacgccg ccaggggaaa
    31081 gacccaaagt ccgcaaaatc gcaatgtccg cctgcttttc cgtaaccgcc atcaccaagg
    31141 aagagacaag gttgaacgcc gccacggcga taatcagcgt caggatgatg aacatcatcc
    31201 gtttttccaa ctcgaccgcc tcaaaatagc tgcggttgtt gtacgtccaa tcgcgcaccc
    31261 aaaccgcgtc cctttgcgcc tccggaatca gtgttgccgt caaggcgggg gcgttttgcg
    31321 ggtcggcgag tttcagccgc agccccgcaa cttccttatc caagcggtac agtacgcgcg
    31381 cgtcttggat atgcgtcatc gccaatgaat tgtcaacctc gtaaacaccc gtcttaacca
    31441 gaccgaccac ggtaaactgt ttcaacctcg gtacgactcc ggcaggggta acgttgccct
    31501 ccggcgtgat gacggtaact ttattgccga cttccgcccc caaagcctcc gccaagccga
    31561 caccgaggat aatgtcaaac tcgcccggaa tcaggtcttc aaatttgcct gccggcattt
    31621 tgtcgccata ttccaccact ttgcgttctt cagacggcaa aatgccgcgc atctgcacgc
    31681 ccctgatttc gcccgcgttt gccagcagag cctgattgga aacatagggc gcggcagcca
    31741 aaataccttt gcggttttcc gtaaaccgaa gcaggttgcg ccaatccgta tccgtattgt
    31801 cgatatagcc gatttcggcg tgcggcgcga cattcaggag ctgcccgcgt atttctttct
    31861 gaaagccgtt cataaccgac aagacgacaa tcagcgcggt tacgcccaag gcgattccgg
    31921 caatcgaaac catcgtgata aacgacataa agccgttgcg ctttttcgcc ctgagatacc
    31981 tcaagcctat ccaagcctct agagaaaaca taacgctacc ttaaaaatgt ctgcaaacgt
    32041 gccgccccgg acggcggttt gggggcggcg gcaaaagttt attgtaccgt aaaaccccgg
    32101 cagcgtccga acgcccgagt gcgggggacg gggcggcaga gcgggcggaa aacttgcaca
    32161 acgcgtcaaa ctgccctatc ctttccttca gaaaaaccgt ttcttgagga aaacaatgaa
    32221 tatccgaact gcttttgctt tgtgcgccat cgccttatcc gccgctgcgg ctgcctacgc
    32281 caaagaaatc aaaatcgatg ccaacaacac gccttattcc gaagccgacg cgcaaaagct
    32341 ggcggcaacg gcagtcggta tgggtgttaa ggaacctatc agcctgaacg gcggcagcgg
    32401 cagcattacc gtgtccggca gcagcgcgac gcagtgcgtg ttcaaagtcg gcaacggagg
    32461 cgcattgcag attcaagggc taaactgcaa gtaaaccgcc cggaaaaatg ccgtctgaag
    32521 gcttcagacg gcattttgca ttggcggcgt tatgccccgc cttctttaat caggcggcgt
    32581 tcgtacaccg cctgcgccag cgttcccgca tcgacatatt ccaattcgcc gcccaagggg
    32641 atgccctgcg acagcctgct gactttgtaa ggcaggtttt taaaaaactc ggacaggaca
    32701 tacgccgtcg cattgccttc tgcggtaaaa gcggttgcaa taatgatttc ttcgacttcc
    32761 ccgccgccca gccgttgcgc cagcctgtcc aatgcgatgg cggatacgtc cattcccaat
    32821 gccgtattga tttgccccat caggacgaaa tacagcccgt cgtggcagtt tgccgcttcc
    32881 atattcgaca cgtcggcagg catatgcacc accatcagcc gccgcccgtc gcgtgtttca
    32941 tcggcacaaa tatcgcacaa tccgccttcg caaaacgtgt tgcacatcgc gcaatggtaa
    33001 acctgcttca atgccgtctg caaggcatcc accagttttt cagcctcttt gcgcttgtgt
    33061 tggagcaaat gatacgctat ccgctgtgcc gatttcggcc caacgttggg taaaaccttc
    33121 agcgcgtcga tcaatccttg gaaggcatct tgttttttgt ggctcatcat attccgccgt
    33181 atgggaaaac ggccggaata ttccgaccgt tattttgtca acaaaagtgt caattactga
    33241 ccgtcgccgt tgtcgaccga ttgcgctcct ttggtctgtt tgattttgcc gttgaaataa
    33301 cgtatcaaca agtcgaaagt attggcagac tgctgttgcg ccaaagcctg ttttgcaagc
    33361 ggaagctgtg cggcgatatc atccggcggg gttacagcct gtacttcgac aatcacgggt
    33421 gccggcagac cgatcagcct gacgtaggcg ggtttgccgt ttgccggttt tgctttcagc
    33481 agttccgcat aagcctcggg cggcatggac tgccttgcct gctgtgcgcc caaaacggac
    33541 acttccgacc atttcacgtc aacagccttg ccgccgttca gttgggtaag cacgtctttt
    33601 gccttgtttt cggcaagttt ggcggcttcg gtacggatat aagcctgacg taccgcgtct
    33661 ttggcttcgg caaacggcag ggttttctct tcgcggactt ctttggcgcg gacgacccac
    33721 gcggtttcgc tgttgatggt cagcacttcg gaattgtgtt ttttcttcaa tacgtcgtcg
    33781 ctgaatacgg cattgatcag gttttcgggc ataccggaca tttgcgcatc ctgcctgctc
    33841 agccaagttt cttgggtttc gactttcaaa ccgctgtttt tggcggcttc ggcaagcgag
    33901 gaaggatggt tgaacgcgtc atcgcccagc ttttcttttg ccttattgaa gtcggcaacc
    33961 gcctttttca ttttcaattc gttttctacg gcggcttttt cctgctcgaa agaaggtttg
    34021 gcttcatttg ccggcaaacg cgccacgcgc tcttcaaacg catttttcac ttctgtttcg
    34081 ctgacggtct gtttgtctgc aaagtctttc agattcaagg cgacatattc caatttgacc
    34141 gctttgggaa gcaggtagtc ttttttgttt gcgttataaa acttctgcaa atcggcttca
    34201 gacactttga cttgggcgat gaattcgtcg gggttgaaag tgtgcgaacg gatggtgcgg
    34261 ttgacctgcg tcagcctgat cagctgttcc gcctgcgcgt cgccgaccaa tacgccgttt
    34321 tggacgaggt ttaccaaatt ctgcaaggca aactgatcgc ggatttcttc gacaaactgg
    34381 tcttcagaca tatggcgttg cgacaggtag cggtttaaaa gcgcgtggtc gaatttgccg
    34441 tttgcgtcgt ggaaattggg atcatccacg ataatctgct tgatttgttc ggaagaaacc
    34501 gaaatgccca tcagcttcgc gccctgtttc aggtaggcgc gttgcagcag ggattggaac
    34561 accgcgtcgc gcgaagggcc gccgccgtcc gcctgttcgt tctgtatggc gttgttgatg
    34621 gagtggtcgc tgattttttc gtcgcccact tggacgatgt agtcggcacc cggatgggat
    34681 accgtgctga ccccgaagcc gacgaaggtt aatgcaatca ggcccaaaag gacttgggcg
    34741 ggcgttctgt atttttcgat ggaatggaac atattttaaa tcgggatata gaatgggaac
    34801 gggaaattca agtcgggtat tgtaacggtt tttatccctg tctgcacggg gcttgccggt
    34861 tgaagatgcc gtcgtaggtt tcttcgctga agccggcgta aaccttgccg ccgcactcca
    34921 atacgggacg cttgatcagg ctcggcattt cggacatcag tttgacggcc tccgctgtcg
    34981 agaacagggc tttttgctgt gtttcggcat cgagtttgcg ccagcttgtc ccgcgtttgt
    35041 tgagcagggt tgccaaaggc acttgttcca gccacgagca gatttccgct tcggaaggca
    35101 cctgtttttt aaaatcccga aattcaaact ccaagccgta tccggcaagc cggtttttgg
    35161 cttttttgac cgtatcgcaa tttgggatgc cgtgaaggat tatcatttgg aaaccttttg
    35221 tctgaaataa taaaacggat attttactat aagtgtctga aaatttgccc gtctgtttca
    35281 gacggcgggg cggttatgtt acaatccgaa aattcgaaaa atttaatctc ttgttcaata
    35341 aaggctttac caatcatgat ttctaccaac ggcatcacca tgcagttcgg cgcgaagccg
    35401 ctgtttgaaa acgtatccgt caaattcggc gaaggcaacc gatacggttt gatcggcgcg
    35461 aacggctcag gcaaatctac tttcatgaaa atcctcggcg gcgatttgga acagacggcc
    35521 ggcgaagtgg cgattgaaaa cggcgtgcgt ttgggtaaat tgcgccaaga ccagtttgcc
    35581 tacgaagata tgcgcgtgct ggacgtggta atgatgggtc acaccgaaat gtgggcggcg
    35641 atgaccgaac gtgatgcgat ttacgccaat cccgaagcca ccgaagacga ctacatgaaa
    35701 gccgcagaac tggaagccaa gttcgccgaa tacgacggct acaccgccga agcgcgtgcc
    35761 gccgagttgt tgagcggcgt gggcatttcc gaagatttgc acaatgcaac catggcagaa
    35821 gttgccccgg gcttcaaact gcgcgtattg ctggcgcagg cgctgttctc caagccggat
    35881 gtattgctct tggacgaacc gaccaataac ttggacatta ataccatccg ctggttggaa
    35941 ggcgtgttga accaatacga ctccacgatg attatcatca gccacgaccg ccactttttg
    36001 aacgaagtct gcactcacat ggcggatttg gactacaaca ccatcaccat ctatccgggc
    36061 aactacgacg actacatgct cgcctccgcc caatcgcgcg aacgcgccct gaaagacaat
    36121 gccaaagcca aagagaaact gcaagagctg caagagttcg tcgcccgctt ctctgccaac
    36181 aaatccaaag cccgtcaggc aaccagccgt ctgaaacagg ccgacaaaat caaatcggag
    36241 atggtcgaag tcaaaccttc cacccgtcaa aacccgtata tccgttttga agccgatgaa
    36301 aaagccaagc tgcaccgtca ggctgtggaa gttgaaaaac tggcgaaacg ctttgaaacc
    36361 cagttgttta aaaacctgaa cttcatcctt gaagcgggac aacgcctcgc catcatcggc
    36421 ccgaacggcg cgggcaaatc caccctgctg aaactcttgg ccggcgcgta caaccccgaa
    36481 tattcagacg gtctgctgcc ggacgaaggc agcatcaaat gggcggaaaa agccagcgtc
    36541 ggctactatc cgcaagatca cgaaaacgac ttcgatgtcg atatggattt gagcgaatgg
    36601 atgcgccaat gggggcagga cggcgacgac gaacaagtca tccgcggcac tttggggcgt
    36661 ttgctcttcg gcagtaacga tgtcgtgaaa aaagtgaagg ttctctccgg tggtgaaaaa
    36721 ggccgtatgc tttacggcaa actgttgctg ttgaaaccca atgtcttagt catggatgaa
    36781 ccgaccaacc acatggacat ggaaagcatc gaatccttga acatggcgct ggaaaaatac
    36841 aacggcacgc tgattttcgt ctcccacgac cgtcagttcg tttcttccct ggctacccaa
    36901 atcattgaat tggacggcaa aggcggatat gaacactact tgggcgatta cgaaagctat
    36961 ctcgagaaaa aaggcgtagc ataaccgccg gttggaacaa tgccgtctga agcggcttca
    37021 gacggcattg ttgataactt taaaatagga agcatatgca gacttatctc gtcggcggtg
    37081 ccgtccgcga ttatcttttg ggcttgcccg tcaaagaccg cgattgggtg gtcgtcggcg
    37141 cagacgcaca aaccatgctg gcgcaaggct tccagccagt cggcaaagac tttcccgtgt
    37201 ttctccatcc cgaaacacac gaagaatacg ccctcgcccg caccgagcgc aaaaccgcca
    37261 aaggttacgt cggtttcagt ttccacgccg acaaagacgt tacgctggag caggatttga
    37321 tgcgccgcga cctgaccatc aacgcgatgg cgcaagatgc ggacggcaag attatcgacc
    37381 ctttcggcgg acaacgggat ttggcggcag gcattttgcg ccacgtttcc ccagcctttg
    37441 ccgaagaccc cgtccgcatc ctgcgtactg cccgctttgc cgcgcgttac aagtttgaaa
    37501 tcgccgaaga aaccataaag ctgatgcggc agatggtgga aaacggcgaa gcggacgcat
    37561 tggttgccga acgcgtctgg caggagtttg cgaaaggttt gatggaaaaa aatccgcgca
    37621 aaatgattga agtgttgcgc gaatgcggcg cgctcaaagt cttgctgccc gaagtcaatg
    37681 ccctcttcgg cgtgccgcaa cgcgccgact accatcccga aatcgacagc ggcatccata
    37741 ccctgatgac gctgcaacgc gccgccgata tgggtttgag cctgcccgaa cgctatgccg
    37801 ccctgctgca cgacttgggc aaagccaaaa caccgtccga catcctgccg cgccaccacg
    37861 gacacgacct cgccggtgtc gaacccgtgc gcgaagtcaa tcagcggctg cgtgcgccga
    37921 aacattgcgc cgagcttgcc gaacttgtct gccgttggca cattattttc caccaagtcg
    37981 gacagcttaa aagccaaacc attctgaacg ttttgaaaaa aaccgacgct ttcagacgcc
    38041 ccgaacgctt tcagacggca ttgaacgtct gcattgccga cacgcaaggc cgtctgaacc
    38101 gcgaacacac gccctacccg caacgcgcgc actggctcgc cttactcgaa gccgccaatc
    38161 aggcggattc gggcaaaatc gccgccgaat gccgctcgca gggaaaagcg caccttatcg
    38221 ccgaacaaat cgaccgggcg cggctggcac aaatcgcccc attgcaaaaa gcgtttcgag
    38281 cggcgcaaga caaaacagaa aaacattaaa acgtccaatg cagcca
//
LOCUS       DAXHSF010000003        33799 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.003, whole genome shotgun sequence.
ACCESSION   DAXHSF010000003 DAXHSF010000000
VERSION     DAXHSF010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 33799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33799
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.003"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(1066..1734)
                     /locus_tag="ACFM8S_000098"
     CDS             complement(1066..1734)
                     /locus_tag="ACFM8S_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFC6313245.1"
                     /translation="MKNLAIFDLDNTLINTDSDHAWPQYLIKKGLVDAAETEAQNEKF
                     YRDYQNGCLDIDAFLKFHLAPLARYSKEELAEFHREFMAEYIIPHISPMQRMLVQSHQ
                     MAGDETLVISSTNEFIITPVCRLFGITNIIGTQLETGSDGRYTGNYIGTPSLKEGKIT
                     RLNQWLAERGETLESYGKTYFYSDSKNDLPLLRLVSEPVAVNPDAELEEEAKEKGWPV
                     LNFK"
     gene            complement(1731..2399)
                     /gene="hda"
                     /locus_tag="ACFM8S_000099"
     CDS             complement(1731..2399)
                     /gene="hda"
                     /locus_tag="ACFM8S_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HFC6313246.1"
                     /translation="MNQLIFDFAAHDYPSFDKFLGTENAELVYVLRHKHGQFIYVWGE
                     EGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQAL
                     LFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDALA
                     SMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQ
                     QETQ"
     gene            complement(2504..4175)
                     /locus_tag="ACFM8S_000100"
                     /pseudo
     CDS             complement(2504..4175)
                     /locus_tag="ACFM8S_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707572.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(4464..5111)
                     /locus_tag="ACFM8S_000101"
     CDS             complement(4464..5111)
                     /locus_tag="ACFM8S_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HFC6313247.1"
                     /translation="MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILVVPPL
                     NESPDVNGTWGVLASTAAPLSEAGYYVFPAAVVEETFKQNGLTNAADIHAVRPEKLHQ
                     IFGNDAVLYITVTEYGTSYQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSN
                     SGLLGALVSAVVNQIANSLTDRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK"
     gene            complement(5108..5476)
                     /locus_tag="ACFM8S_000102"
     CDS             complement(5108..5476)
                     /locus_tag="ACFM8S_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
                     /protein_id="HFC6313248.1"
                     /translation="MNPKTLSRLSLCAAVLALTACGDNGQKSLYYYGGYPDTVYEGLK
                     NDDTSLGKQTEKMEKYFVEAGNKKMNAAPGAHAHLGLLLSRSGDKEGAFRQFEEEKRL
                     FPESGVFMDFLMKTGKGGKR"
     gene            complement(5486..6157)
                     /locus_tag="ACFM8S_000103"
     CDS             complement(5486..6157)
                     /locus_tag="ACFM8S_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HFC6313249.1"
                     /translation="MKTVSTAVVLAAAAVSLTGCATESSRSLEVEKVASYNTQYHGVR
                     TPISVGTFDNRSSFQKGIFSDGEDRLGSQAKTILVTHLQQTNRFNVLNRTNLNALKQE
                     SGISGKAHNLKGADYVVTGDVTEFGRRDVGDHQLFGILGRGKSQIAYAKVALNIVNVN
                     TSEIVYSAQGAGEYALSNREIIGFGGTSGYDATLNGKVLDLAIREAVNSLVQAVDNGA
                     WQPNR"
     gene            complement(6322..7041)
                     /locus_tag="ACFM8S_000104"
     CDS             complement(6322..7041)
                     /locus_tag="ACFM8S_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC6313250.1"
                     /translation="MATLTDKTILVTGASQGLGEQVAKAYAAAGATVILVARHQKKLE
                     KVYDAIVEAGHPEPFAIRFDLMSAEEKEFNQFAATIAEATQGKLDGIVHCAGYFYALS
                     PLDFQTVAEWVNQYRINTVAPMGLTRALFPLLKQSPDASVIFVGESHGETPKAYWGGF
                     GASKAALNYLCKVAADEWERFGNLRANVLVPGPINSPQRIKSHPGEAKSERKSYGDVL
                     PAFVWWASAESKGRSGEIVYL"
     gene            complement(7125..8438)
                     /gene="hpnE"
                     /locus_tag="ACFM8S_000105"
     CDS             complement(7125..8438)
                     /gene="hpnE"
                     /locus_tag="ACFM8S_000105"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HFC6313251.1"
                     /translation="MMNTPHPRPKIAVIGAGWAGLSAAVTLARHADVTLFEAGRQAGG
                     RARTLAGNTDGFGFLDNGQHILLGAYRGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQ
                     FRALPLPAPLHILGGVLLARRAPTAFKAKLLADMSDLQKSARLGQPDTTVAQWLKQRN
                     VPRAAVMQFWQPLVWGALNTPLETASLRVLCNVLSDGVLTKKSGSDYLLPKQDLGAIV
                     AEPALAELQRLGADIRLETRVCRLNPLPDGKVLVNGEAFDAAVPATAPYHAAALLPEG
                     TPEHVQTAYQNLRYHAITTVYLRYAEPVRLPAPLTGLADGTVQWLLCRGRLGLPENEV
                     SAVISVSDRVGAFANRAWADKAHADLKRILPHLGEPEAVRVITEKRATTAADAPPPDL
                     SWLHRHRIFPAGDYLHPYYPATLEAAVQSGFASAEACLQSLNDAV"
     gene            complement(8517..9389)
                     /gene="hpnD"
                     /locus_tag="ACFM8S_000106"
     CDS             complement(8517..9389)
                     /gene="hpnD"
                     /locus_tag="ACFM8S_000106"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HFC6313252.1"
                     /translation="MKGLDYCRQKAEESRSSFLSGFRFLTQEKRDAVTVLYAFCRELD
                     DVVDECSNPDVAQATLNWWRGDLDKVFGGAMPEHPVNQALRQVKETFKLPKYELEALI
                     DGMQMDLVRARYGSFEELKLYCRRVAGVVGCLIARILGFSDGKTLEYADKMGLALQLT
                     NIIRDVGEDARRGRIYLPMEEMQRFDVPASVILQCSPTGNFAELMAFQIKRARETYRE
                     AVSLLPAADKKAQKVGLVMAAVYYELLNEIDRDGAQNVLKYKIALPSPRKKRIALKTW
                     LFGFKPRPGTPERA"
     gene            9560..11422
                     /gene="hscA"
                     /locus_tag="ACFM8S_000107"
     CDS             9560..11422
                     /gene="hscA"
                     /locus_tag="ACFM8S_000107"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFC6313253.1"
                     /translation="MALLQISEPGMSAAPHRHRLAAGIDLGTTNSLVATVRSGSAACL
                     PDAEGRVTLPSVVRYLENGGIEVGKTALSAQKTDPLNTVSSAKRLIGRTLADLHQNTH
                     YLPYRFGDNQRVIELHTRQGVKTPVEVSAEILKTLKSRAEETLGGDLVGVVITVPAYF
                     DDAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNASEGTFVVYDLGGGTFDVSVL
                     QLTKGLFEVKATGGNSALGGDDFDHRLFCRLLEQNGLSQLNEQDSQLLLSLVRAAKEQ
                     LTTQTEARIQATLSDGMAIDTSISRAEFHNLTQHLVMKTLEPVTQALKDAGVGKNEVK
                     GVIMVGGSTRMLHVQQAVATFFGQTPLNNLNPDEVVALGAAIQANVLAGNKADGEWLL
                     LDVTPLSLGLETYGGLAEKIIPRNSTIPTARAQDFTTFKDGQTAMTIHVVQGERELVA
                     DCRSLAKFTLRGIPPMAAGAARIRVTFQIDADGLLSVSAQEQSTGVHAQIEVKPSYGL
                     DDDTITQMLKDSMSNAAEDMAARARAEAVVEAESLTDAVNAALELDSDLLDAEELQQI
                     RQGIADLQGRLKDGKAEDIRAAAAKLGSITDNFAAKRMNRNIQRALTGQSVDNI"
     gene            11504..12160
                     /locus_tag="ACFM8S_000108"
     CDS             11504..12160
                     /locus_tag="ACFM8S_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFC6313254.1"
                     /translation="MEKAGIVYLMKTVIKGVYKIGISDVSNFEGRMRHLENNGYANVA
                     GLERILAVKTDNYKEKENLLHEIFSKSRIGDTELFAVDENLVKRLFLSLRGEIVFPKN
                     ETAESEFEKSVHERRQEGNAGSGRKQLLDLVRRGHREYPYALPRLLAGAASYKPKKSK
                     IRLFKEAYFGKSGTRLTDEIADGIHIYTCFSRADLEKAYSEYLELFKSESDAEGRKPQ
                     "
     gene            12197..12946
                     /locus_tag="ACFM8S_000109"
     CDS             12197..12946
                     /locus_tag="ACFM8S_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HFC6313255.1"
                     /translation="MMGDSVIYYVEPVNRAGERARKTFKYFWRELFWERRRIIPTLDF
                     AMVKVPFFQDGEDGEICEHMWIDDIYFDGLYIYGVLNNEPGELTNVEQGESICVPVDD
                     ISDWIFVCNGIPYGGFTVQAMRGQMTEEERTEHDAAWGIDFGDPWQVLLAYEEKEHPE
                     NLEEHPMCRNCIDDFRQQLSQNPDFLHEQDEDGYTPLHHDAIAGNALMVKAMLEYGAN
                     PASTTSEGYTALDFARLTGWQNVADLLEPRH"
     gene            13000..13341
                     /gene="fdx"
                     /locus_tag="ACFM8S_000110"
     CDS             13000..13341
                     /gene="fdx"
                     /locus_tag="ACFM8S_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFC6313256.1"
                     /translation="MPKITVLPHTTLCPEGAVIEDAPEGKTVLDVLLDHDIEVDHACE
                     KSCACTTCHVIIRKGFDSLEEPTELEEDLLDQAWGLEADSRLSCQAVVAGEDLIVEIP
                     KYTINHAREEH"
     gene            complement(13458..14045)
                     /locus_tag="ACFM8S_000111"
     CDS             complement(13458..14045)
                     /locus_tag="ACFM8S_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFC6313257.1"
                     /translation="MNNMFAAKLSKLVYTASDHPDPLSEMGEFDRLILLIRKLYQILD
                     GQHTLSRVTVCLHYRSRRGLIALDKAAAGCDAAMLRAQRWRIYTQIAERLAGSDGGFT
                     VTAESVSAACPELEGRYLELVRRAAVSFGFTQGWENPAGGQEKTQAVPDAASGHSVRK
                     PIKINIEAKVECYPAYRPTAALKVTRRRFFRLRNR"
     gene            14258..14455
                     /gene="iscX"
                     /locus_tag="ACFM8S_000112"
     CDS             14258..14455
                     /gene="iscX"
                     /locus_tag="ACFM8S_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFC6313258.1"
                     /translation="MKWTDTRRIAEELYDLHGETIDPRTVRFTQLRDLIMALPEFDDD
                     PARCGERILEAVQQAWIDEAE"
     gene            complement(14523..14924)
                     /locus_tag="ACFM8S_000113"
     CDS             complement(14523..14924)
                     /locus_tag="ACFM8S_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFC6313259.1"
                     /translation="MKDKHDSSAMRLDKWLWAARFFKTRSLAQKHIELGRVQVNGSKV
                     KNSKTIDIGDIIDLTLNSLPYKIKVKGLNHQRRPASEARLLYEEDAKTAALREERKQL
                     DQFSRITSAHPDGRPTKRDRRQLDRLKKGDW"
     gene            15135..16094
                     /locus_tag="ACFM8S_000114"
     CDS             15135..16094
                     /locus_tag="ACFM8S_000114"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HFC6313260.1"
                     /translation="MKPVFLDFEQPIAELTNKIDELRFVQDESAVDISDEIHRLQKKS
                     NDLTKSIFSKLTPAQISQVSRHPQRPYTLDYIEALFTDFEELHGDRHFADDYAIVGGL
                     ARFNGQSVMVVGHQKGRDTKEKIRRNFGMPRPEGYRKALRLMKTAEKFGLPVMTFIDT
                     PGAYPGIGAEERGQSEAIGKNLYELTRLHVPVLCTVIGEGGSGGALAVAVGDYVNMLQ
                     YSTYSVISPEGCASILWKTAEKAADAAQALGITADRLQKLDLVDTVIKEPLGGAHRDF
                     GQTMKNVKAVLEKQLHEAQSIPLADLLSRRFDRIMAYGKFSEQ"
     gene            16162..17472
                     /gene="tilS"
                     /locus_tag="ACFM8S_000115"
     CDS             16162..17472
                     /gene="tilS"
                     /locus_tag="ACFM8S_000115"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFC6313261.1"
                     /translation="MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVRA
                     GKKGGFIPDALHIHHGLSPRADDWADFCQNYCDMLGVGLETVKICVEKNGLGIEAAAR
                     QKRYAAFAEKGFDVLALAHHRDDQIETFMLAVARGGGLRALAAMPAVRPFGEKGIIWR
                     PLLPFSRQDIWDYAQKHGLPNIEDESNSDTAYLRNRFRHRILPELSAQIPHFGRHLLN
                     NVRALQEDLALLDEVVVQDCRWVCGAGYFDTARWLTFSPRRKTHILRHFLKENGIPVP
                     NQNALADIARVLTEAKTGRWNVQGFELHHYAGRLFVFRPEKTDKLRFLKDRQISGNLR
                     EILAGQGFVLKRHPFGLPGHLLEQDGILRTVAASDRLAVGGIHKDVKKILQEKRVLPA
                     LRPFWPVVADKDNRPLALANYGTDFQISVSDGILPVHPDFPVLF"
     gene            17523..18308
                     /locus_tag="ACFM8S_000116"
     CDS             17523..18308
                     /locus_tag="ACFM8S_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFC6313262.1"
                     /translation="MKHISSANNEHIRHLHRLLSQGKFRRQYAQTVLEGVHLLQVFLQ
                     SGGMPVGVYIPEAKMPSEEVRKLTAVLPEDGVFSVSDGILKKISSLTCADDVLALIDI
                     PDAGALPAGGDCVVLDGVQDPGNVGTVLRSAAAAGIGAVVLGKGCADAWSPKVLRAGM
                     GAHFLSEIYPQADLEIWLARYEDRVFATALREEKQAVLYNEDLREPTAWVFGNEGAGV
                     GKAVLDRADKCVRIPMHDATESLNVAMAATICLFEQMRQRSAY"
     gene            18318..18515
                     /locus_tag="ACFM8S_000117"
     CDS             18318..18515
                     /locus_tag="ACFM8S_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313263.1"
                     /translation="MPSEKIYYGVLIFLCIASMLLSPFFYAGALKPKKAALRKDGQWK
                     LILLSNTVAAAVLAWVWWKWF"
     gene            18777..19130
                     /locus_tag="ACFM8S_000118"
     CDS             18777..19130
                     /locus_tag="ACFM8S_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313264.1"
                     /translation="MKNCKFFYDPTRAIYDSGADYLTREKHRLVVIANSAWGLLLNLS
                     CYYDEVLEKRKIPFGKQEIDDDMDKVSAHKRKFKDISEIKVGDGWEYPFNYEQGMKEL
                     DEVLLKYIPFFEEER"
     gene            19142..19447
                     /locus_tag="ACFM8S_000119"
     CDS             19142..19447
                     /locus_tag="ACFM8S_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313265.1"
                     /translation="MRVSKIIGSMLLVTAVQTVFSANVYACRHNGKTSYSQTPGKHCT
                     NAGLGRDRVYSSVRPAVKDRAEDAGVGDYSDTVRDEHVQNPKENMHKDASNTGAKTH"
     gene            19509..20885
                     /gene="mpl"
                     /locus_tag="ACFM8S_000120"
     CDS             19509..20885
                     /gene="mpl"
                     /locus_tag="ACFM8S_000120"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFC6313266.1"
                     /translation="MKHIHIIGIGGTFMGGIAAIAKEAGFEVSGCDAKMYPPMSTQLE
                     ALGIGVHEGFDAAQLEEFKADVYVIGNVAKRGMDVVEAILNRGLPYISGPQWLAENVL
                     HHHWVLGVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFSVSARLPQTPRQDPN
                     SQSPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFADLGAIQTQFHHL
                     VRTVPSEGLIICNGQQQSLQDTLDKGCWTPVEKFGTEHGWQAGEANADGSFDVLLDGK
                     TAGRVKWDLMGRHNRMNALAVIAAARHAGVDIQTACEALSAFKNVKRRMEIKGTVNGI
                     TVYDDFAHHPTAIETTIEGLRQRVGNARILAVLEPRSNTMKLGTMKAALPESLKGADQ
                     VFCYAGGVDWNVAEALAPLGGKLHVGKDFDAFIAEIVKHTEAGDHILVMSNGGFGGIH
                     TKLLDALR"
     gene            21092..22144
                     /gene="bioB"
                     /locus_tag="ACFM8S_000121"
     CDS             21092..22144
                     /gene="bioB"
                     /locus_tag="ACFM8S_000121"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFC6313267.1"
                     /translation="MTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLDLIYQAA
                     EIHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAK
                     IAKSRGASRFCMGAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKE
                     AGLDYYNHNLDTDPDRYNDIIHTRRHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAER
                     AGLIASLANLDPQPESVPINRLVKVEGTPLADAEDLDWTEFVRTIAVARITMPQSYVR
                     LSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFEPE
                     GEVAEVEKASGIKVDY"
     gene            complement(22290..22856)
                     /locus_tag="ACFM8S_000122"
     CDS             complement(22290..22856)
                     /locus_tag="ACFM8S_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HFC6313268.1"
                     /translation="MNIKYSGIENRFETAILKKNGVRSGFVSFAPNLAAVKLNDYAKV
                     RKRITKTKQKADIVIVMFHGGAEGKQAEHLPFDTEIFYGENRGNVVEFARLAVDSGAD
                     VVFGQGSHVTRAVELYRDRFISYSGGNFATYGAINTSGISGIAPIFKIITDKQGKFIS
                     GTIIPITQADDKIPKIDPEKPLSGGLFI"
     gene            complement(22980..23219)
                     /locus_tag="ACFM8S_000123"
     CDS             complement(22980..23219)
                     /locus_tag="ACFM8S_000123"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313269.1"
                     /translation="MGVCTFFRRTAFVKQRAFEVADGNVRVLQGRFDVFQNIGIGQVI
                     DRIIAAEHNVAHADNGNGIGCLFVRRILSVHIAGI"
     gene            23206..23439
                     /locus_tag="ACFM8S_000124"
     CDS             23206..23439
                     /locus_tag="ACFM8S_000124"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HFC6313270.1"
                     /translation="MQTPKICYAFRTPSAYGQYLADAGFDYLSFANNHSNDFGAQGIT
                     ATAASGSSFTYSIALKPLPITRPKLRKIPPSPR"
     gene            complement(23502..25361)
                     /gene="ilvD"
                     /locus_tag="ACFM8S_000125"
     CDS             complement(23502..25361)
                     /gene="ilvD"
                     /locus_tag="ACFM8S_000125"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFC6313271.1"
                     /translation="MPEYRSKTSTHGRNMAGARALWRATGVMETDFGKPIIAVANSFT
                     QFVPGHVHLHNMGQLVAREIEKAGAIAKEFNTIAIDDGIAMGHSGMLYSLPSRDLIAD
                     SIEYMVNAHCADALVCISNCDKITPGMLIAAMRLNIPTIFVSGGPMEAGKVIGVANIQ
                     PERRLDLIDAMIESADDNVSNRQVEEVEQNACPTCGSCSGMFTANSMNCLTEALGLSL
                     PGNGSYLATHAGRKELFLEAGRMIVEITKRYYEQDDETVLPRSIATKKAFENAMTMDI
                     AMGGSTNTILHLLAVANEAGVDFKMADIDRLSRVVPCICKTAPNNHDYYMEDVHRAGG
                     IFAILKELDKAGKLHTDVYTIHALTLKDAIEKWDVTNPENTRAIERFKAAPGGVRTTQ
                     AFSQNRMWKTLDLDREKGCIRDVAHAYSQDGGLAVLFGNIAERGCVVKTAGVDESILK
                     FTGRARVFESQEDAVEGILGNQIVAGDIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KACALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVHEGDTVEIDIPNRSIHLAISDE
                     ELAARRAEMEARGSKAWKPKNRDRYVSAALRAYGAMATSADKGAVRDVAQIER"
     gene            complement(25534..27303)
                     /gene="cysI"
                     /locus_tag="ACFM8S_000126"
     CDS             complement(25534..27303)
                     /gene="cysI"
                     /locus_tag="ACFM8S_000126"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFC6313272.1"
                     /translation="MTVQTKTKGLAWQEKPLSDNERLKTESNFLRGTILDDLKDPLTG
                     GFKGDNFQLIRFHGMYEQDDRDIRAERAEAKLEPLKFMLLRCRLPGGIIKPSQWIELD
                     KFARENSHYRSIRLTNRQTFQFHGVPKAKLQTMHRLLHKLGLDSIATAADMNRNVLCT
                     SNPIESELHRQAYEYAKKISEHLLPRTRGYLDVWVDGKKVQSSDDFLQEDEPILGKTY
                     LPRKFKTAVVIPPLNDVDCYGNDLDFVAVSDGNGQLAGFNVLAGGGLSMEHGNTKTYP
                     NISLELGFVPPEHALKAAEAVVTTQRDFGNRSDRKNARTRYTIQNMGLDNFRAEVERR
                     MGMPFEPVRPFKFTGRGDRIGWVKGIDGNWHLTLFIESGRLVDEGGKQLLTGVLEIAK
                     IHKGDFRITANQNLIVANVAEADKAKIEELARTYGLIRNDVSKLRENAMSCVSFPTCP
                     LAMAEAERVLPDFIDDLDKIMAKHGTSDDYIVTRITGCPNGCGRAMLAEIGLVGKAVG
                     RYNLHIGGDREGVRIPRLYKENITLPEILAELDDLIGKWAAERNIGEGFGDFAIRTGI
                     VKPVLNAPVDFWDASKAVAIARA"
     gene            complement(27330..29144)
                     /locus_tag="ACFM8S_000127"
     CDS             complement(27330..29144)
                     /locus_tag="ACFM8S_000127"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HFC6313273.1"
                     /translation="MSEHDMQNTNPPLPPLPPEITQLLSVLDAAQWAWLSGYAWAKAG
                     NGASAGLPALQTALPAAEPFSVTVLSASQTGNAKSVADKAADSLEAAGIQVRRAELKD
                     YKAKNISGERRLLLVTSTQGEGEPPEEAVVLHKLLNGKKAPKLDKLQFAVLGLGDSSY
                     PNFCRAGKDFDKRFEELGAKRLLERVDADLDFTASADGWTGRIVARLKEEAAKNRATP
                     APQTTPPAGLQTAPDGRYCKADPFPAALLANQKITARQSDKDVRHIEIDLSGSDLHYL
                     PGDALGVWFDNDPALVREILDLLGIDPATEIQAGGKTLPVASALLSHFELTQNTPAFV
                     KGYAPFADDDELDRIAADNAVLQGFVQSTPIADVLHRFPAKLTAEQFAGLLRPLAPRL
                     YSISSSQAEVGDEVHLTVGAVRFEHEGRARVGGASGFLADRLEEDGTVRVFVERNDGF
                     RLPEDSRKPIVMIGSGTGVAPFRAFVQQRAAENAEGKNWLIFGNPHFAADFLYQTEWQ
                     QFAKDGFLHRYDFAWSRDQEEKIYVQDKIREQAEGLWQWLQEGAHIYVCGDAAKMAKD
                     VEAALLDVIIGAGHLDEEGAEEYLDMLREEKRYQRDVY"
     gene            complement(29141..30427)
                     /locus_tag="ACFM8S_000128"
     CDS             complement(29141..30427)
                     /locus_tag="ACFM8S_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit 1"
                     /protein_id="HFC6313274.1"
                     /translation="MTAQHQAPLRFITAGSVDDGKSTLIGRLLYDSKALLSDQIKTLE
                     SGKSKGLKETIDFSILTDGLEAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRN
                     MVTGASTASAAVVLVDASQLDFGAQPLQLLPQTKRHSAILRQLDCPHIVVAVNKMDLL
                     DYSEDKFNAIVEAYRRLAGRLGLKDVHFVPVSALLGDNIVYPGGNMPWYKGEPLLSIL
                     ETLPGADEVSRTADDFYFPVQLVVRQDADKADDFRGYQGRIERGSVAVGQTVRIEPNG
                     LTAKVSEIITPKGEVAQAFAGEAATLRLDRDIDVSRGDLFVDKNSPLAPQKHLEATLC
                     WFDERPLNTARKYLLKHGTQTVPAKVGEIESVLDVRTLEQEAGAESLKMNDIAKVRIN
                     LQKPVTATPYAENTAAGSFILIDEATYGTVAAGMIL"
     gene            complement(30466..31389)
                     /gene="cysD"
                     /locus_tag="ACFM8S_000129"
     CDS             complement(30466..31389)
                     /gene="cysD"
                     /locus_tag="ACFM8S_000129"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFC6313275.1"
                     /translation="MTKTEPNNAQLDWLESESIHIIREVAAECENPALLFSSGKDSVV
                     LLALACKAFRLGSRPVKLPFPLVHIDTGHNYPEVIAFRDAQAAKLNARLIVGRVEDSI
                     AKGTVVLRKETDSRNAAQAVTLLETIEANGFDALMGGARRDEEKARAKERIFSFRDEF
                     GQWDPKAQRPELWSLYNTRLHKGENMRVFPISNWTELDIWQYIARENLELPPIYYSHR
                     REVVRRRGLLVPVTPLTPKMPSETSEILDVRFRTVGDISCTCPVESTASTPTEIIRET
                     AVADISERSATRLDDQASEAAMEKRKKEGYF"
     gene            complement(31427..32167)
                     /locus_tag="ACFM8S_000130"
     CDS             complement(31427..32167)
                     /locus_tag="ACFM8S_000130"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HFC6313276.1"
                     /translation="METTLFKPALWQIPHIGSGGETALAEKTETLKQRLHRIAGSHRD
                     ARFASSLAAEDMVITDLIAGENLNIGIFTLDTGLLHTETLNLLDRLGRAYPHLRIKRF
                     RPVREDADRYVESKGRFAFYDSVEARRECCRIRKIEPLNRAIAGADAWLTGQRREQSA
                     TRTELPFAEYDAGRGIGKYNPIFDWSEHDVWAYILANNVPYNDLYRQGFPSIGCDPCT
                     RPVKAGEDIRAGRWWWEGRNSKECGLHK"
     gene            complement(32178..33629)
                     /gene="cysG"
                     /locus_tag="ACFM8S_000131"
     CDS             complement(32178..33629)
                     /gene="cysG"
                     /locus_tag="ACFM8S_000131"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFC6313277.1"
                     /translation="MNYFPIFANLAGRPVLVVGGGAVAARKISLLLKAGAEVRVAAKH
                     LNAELSALAAENKILWLAEEFRAEHIRTVFLIIAASSDQALNRRVFHLAESCQKPVNV
                     VDDRDHCSFIFPSVIDRNPVQIAVSSSGSAPVLARLLRERLEALLPPSLGDMAEISGR
                     WRDAVKDKLKSVTERRRFWEKQFNGRFAALVKNRQNTLAERELAGQLEQSRQNDQGGS
                     VSLVGAGPGDAGLLTLKGLQEIQQADVVLYDALVSDGILSLVRRDAERIFVGKRARGD
                     RTPQEDTNALMVRLAREGRRVVRLKGGDPFVFGRGGEELETLARHQIPFSVVPGITAA
                     VGATAYAGIPLTHRDYAQSAVFVTGHRKADAPDIEWQTLARSRQTLVIYMGALKAALI
                     AERLQQHGRSPDTPAAVISQGTLPAQKTATGTLANLAELAETAPNPALIVIGEVVGLH
                     EKLAWFGENGEGENRVGQAYPALGGLNAGQRAA"
BASE COUNT         7356 a         8735 c         9698 g         8010 t
ORIGIN      
        1 tcgccttgtc ctgatttttg ttaatccact atacgtccta gattcccact ttcgtgggaa
       61 tgacgggatg tgggtttttg tgcggatttg aaccggtaag ggtggtgtgg gattggtggt
      121 ttgcttagga tcttttggat tgtattttgt atatacattt acttgttgat aaaagataaa
      181 ataaaattag aaactaaaag tgagaaaaaa ttaataataa tagggatgta taaatgtaaa
      241 gactccgttt catagctaag gttatctgaa tatatggaaa aaaagtaaaa gtccataaaa
      301 ctaaaatata tagataatgc taatgataat agaattatca ctttcctaga gtaaggataa
      361 tattttttta tcgtaaaaaa atataaaata ggataaataa ttgccatata gatagatttt
      421 gtaacaaaat taaaaataaa taataaaaat aatgtaataa agtatatttt ttggctatga
      481 aataaaattg tacataattg aacgagcaga tcaaaaaatg aactacatat aacaataaat
      541 aataacgtat ttaccatact aaatttaata ggtctcatta tcatatttaa taaccacttc
      601 atagtatagt ggattaaatt taaaccagta cagcgttgcc tcgccttgcc gtactatctg
      661 tactgtctgc ggctttgtcg ccttgtcctg atttttgtta atccactata aatgcagagt
      721 gggtggaaac actcacttta tggtttgcta cgctctgctc aattagcaac ccgataaccc
      781 aatatggata atagggtaat taatccaatc taatttgtca gcatccgtta atttattgca
      841 aaataaagta ttgaattatg tcgggtgcaa atgacgaaat ataagtttcc gtgcggacgg
      901 atcaagattc ccactttcgt gggaatgacg gtggaaagat tgttgttttt cccgatgaat
      961 tcctgtgttt tttgtttttc cggataaatt cctgtggctt tgagtttttt ggatttcagc
     1021 ctcaatgccg tctgaacgcc gaatcgggct tcagacggca ttgcgtcatt tgaaatttaa
     1081 aaccggccag cctttttctt tggcttcttc ttccagctcg gcatcggggt tgacggcgac
     1141 gggttcgctg acaaggcgca gcagcggcag gtcgtttttg gagtcgctgt aaaaataggt
     1201 tttgccgtag ctttcgagcg tttcgccgcg ttcggcaagc cattggttca ggcgggtgat
     1261 tttgccttct ttgaggctgg gcgtgccgat gtaattgccg gtgtagcggc cgtcagaacc
     1321 ggtttcgagt tgtgtgccga tgatgttggt gatgccgaaa aggcggcaga cgggggtgat
     1381 gatgaactcg ttggttgagg aaatcacaag ggtttcgtcg cctgccattt ggtggctctg
     1441 caccagcata cgctgcatag gcgagatgtg ggggatgatg tattccgcca taaattcgcg
     1501 gtgaaactct gccagctctt ctttgctgta gcgggcgagc ggggcgaggt ggaatttgag
     1561 gaatgcgtcg atgtcgaggc agccgttttg gtagtcgcgg tagaattttt cgttttgcgc
     1621 ttcggtttcg gcagcgtcaa ccaagccttt tttgatgagg tattgcggcc aggcgtggtc
     1681 ggaatcggtg ttgatgaggg tgttgtcgag gtcgaagatg gcgaggtttt tcattgggtt
     1741 tcctgttgtt tcaaaagctg gcgcaaaagc ggcagggtga tgcgtttgcc cattgtgacg
     1801 gcgtagttgt ccagcgtgtc gagcatcatc atcaggctgt ccatatcgcg ccgccagtgt
     1861 ttgagcaggt attcgaaaat ttcggaatcg acggttactt ggcgtgccgc cgccatactg
     1921 gcgagcgcgt cgattttttc ttggtcggtt aagggcttga cttcgtaaac gaggcagtac
     1981 gccatacgcg tccgcaaatc ttcgcggatg acgagctgct ggggcgtgta ttccgaaccg
     2041 agcagcaaaa agcctttgcc gctgttgcgg aagcggttga agatggaaaa aagcagggct
     2101 tgttcttcgt tgcccagctt ttcgacttga tcgacggcga ggtattccgc ctcgaacgcg
     2161 gcatcggtca gcggcatgga ggcggcatcg atataggcgg cgtttttgcc ggcttcgagc
     2221 gcctgtgcga cccacgcctg caaaagatgg cttttgcccg caccttcctc accccagaca
     2281 tagataaact gtccgtgttt gtgtcggagg acatagacca gttccgcgtt ttccgtgccg
     2341 aggaatttgt cgaaactcgg atagtcgtgt gcggcaaagt cgaaaataag ctggttcacg
     2401 gttcggcatt ccgaggggtg gtaaacgggt ttattgtacg ttgttttcgc gtgctttatc
     2461 aatttgaaaa atgccgtctg aaaacggctt cagacggcat cgttcagccg caggcaacgt
     2521 tgccgacatc gaggtgcata ttgtggaacg cgttgagcgt gctgcggtgg ccgatactga
     2581 tgatgatgct gtcgggcagt ttttgcttta aggcgcggta gagcatggct tcggtcggtt
     2641 cgtccaaagc ggcggtagct tcatcgagca agatgacttt gggcttggaa agcagggcgc
     2701 ggacgaaggc gacgcgttgc agttcgcccg gggagagttt gtgttgccag tcgtcggttt
     2761 tatctaattt atcaaccaga taacccaagc ggcaggtgtt catggcttcg attagctccg
     2821 gatgctgttt gtcgatgtcg ggataacaaa ccgcgtcgcg caggctgccc tgtgccgtgt
     2881 acgggcgttg cggcaggaag aggatgtctt gatgcggcgg acggctgact ttgccgttgc
     2941 tgccgaacgg ccaaagccca gccagcacgc gcagcagcga ggttttgccg caaccgctcg
     3001 gaccgcgtat cagcagggaa tcaccgcttt tgaggttgat attgataccg tctaataaaa
     3061 cttcgccgtt gtggcggaac agagcgacgt tttccagtgc aatgccgtct gaaacttcgc
     3121 taatctcggg ttgccgcgcg ctgtgttgtt cttcggtact cagcaaaaag ccgtacagac
     3181 gctccaatcg ggcgcggtag gcggtgaatt tgttgtagaa cattcgaaag aaagaaagcg
     3241 cgttttgcag tcgcgcgaag gcttggacgg tttgctggat gtcgccgatt ttgatttgtc
     3301 cggcaaacag gcgcggagct tgcaaaataa tcgggaagag tttgatgccg ttggtgaaca
     3361 tatcgttaaa gccgcttaag cagacgcttt gccgcgcaat gcgccaacgg ttgcggataa
     3421 tggctttaaa gcggtcggaa agctggtcgt attcatgttt ttcgccgttg taaaacgcca
     3481 cgctttcggc gtggtcgcgc acgcggatga gggaataacg gtagtcgccg ttgagttttt
     3541 cgttttcata attgtaacga atcaaagggt tgcctatcca catggcgata aaggtcgcca
     3601 aaatcacaaa tatatagaca aaccaaacga tgccgtgcgg aatgtcgaag ccgaacacgg
     3661 tcaggatgcc agccaagccc cgcaaaacaa cggcaaattc cagagaagta acgaccgaat
     3721 tgaccatgcc gcgcacaaat tcgatggtcg aagcgataaa ttcctgcgcg tcctgttgga
     3781 tacgctggtc gatgttgtct ggcgcgtggc ggcgcatttg caggcggtag tagtttttgt
     3841 cggcaagcca gcgtgctgtc agcacttcgt tgagccgctc cgaccattta atcgccaagc
     3901 cttgatcgag gaagtcgttg acgacgttgt taaacgcccg tatcagcacc acgcccgcgt
     3961 tcattgcagc aaacatccaa aatgccgaag catttcaaat cctgcatcga gtcgtaaagc
     4021 cctttggaca taaaggtact caacacattc agccgcattt cggttaacac cagcgtaatc
     4081 atcgccgtaa tcagcagcaa gactttgacc gcgcttttca gtgtcagaca aagccaaagc
     4141 ggtgtggaat aaagctcggt ttgccatttc tgcatgggaa atttcttacg gtatcaatgc
     4201 cgtctgaaaa agacgggtac agttgatttt ttgatgaagt ttggggaagt tttgcaggtc
     4261 agggcatatt gcgtgttaat ttaccataca accacgccgg aattaagttt aaatttgaat
     4321 aaaaggttcg ggttctgcaa aatacagaac ccgaaccttg ttcggatatt gaaaccggcc
     4381 gcccgatttt gggcggtgcg gcttgcaagt atcaagattc gcatatgccg tctgaagctc
     4441 ggagaggttc agacggcata tgcttatttg ggctgctctt cgacgaatct cggacctttc
     4501 aagatgccgt tgtgagaata gggcgacagc aggttgtatg cggcggtttt ggaaacctga
     4561 taaccgcggt cggtcaggct gttggcaatc tgattgacca ctgcgctgac caaagccccc
     4621 aacaggccgc tgttgctgtt gttgctgcct tcgcggatgc tggccgaacc cgaccacaac
     4681 tcttttccgt tgcgggaatc gaccagccgt gctttggcgg atacggtcgt cacgctgtct
     4741 aaaatttgat atgaagtgcc gtattcggta accgtaatgt acaaaaccgc atcattgccg
     4801 aaaatctgat gcagtttttc cggccggacg gcgtgaatat cggcggcatt ggtcaagccg
     4861 ttttgtttga aggtttcctc cacgactgcg gcggggaaga cgtaatagcc ggcttcggaa
     4921 agcggcgcgg cggtcgaagc cagtacaccc catgttccgt tgacatcggg cgattcgttc
     4981 agcggcggaa ccaccaaaat tgaagccggt ttgctttcct tgaatgacgt gtagtcgaaa
     5041 tcgggagctt tttgaacttg gcaggcagac agcgccaaca cggcggcaag ccctaaaatc
     5101 aaaggtttca tcgcttgcct cctttaccgg ttttcatcag gaagtccata aatacgcccg
     5161 attcgggaaa cagccttttc tcttcttcaa actggcggaa cgcgccctct ttgtctcccg
     5221 aacgggaaag cagcagtccc agatgggcgt gcgcacccgg ggcggcattc atttttttgt
     5281 tgccggcttc cacaaagtat ttttccatct tttcggtctg cttgcccaac gaagtgtcgt
     5341 cgtttttcaa accttcatag acggtatcgg gatagccgcc gtaataatac agggattttt
     5401 gcccgttgtc gccgcaggcg gtcagagcca agaccgccgc acacagcgac aaacggctca
     5461 aggttttcgg attcatcatt tctccttaac ggttgggttg ccatgcgccg ttgtcaacag
     5521 cctgaaccag gctgttgacg gcttcgcgga ttgccaagtc taaaactttg ccgttcaaag
     5581 tcgcatcgta gccggaagtg ccgccgaaac cgatgatttc acggttggaa agtgcgtatt
     5641 cgcccgcgcc ctgtgcggaa tagacgattt cggaagtatt gacgttgacg atattcagag
     5701 ccacttttgc ataggcgatt tgcgatttgc cgcgacccaa aatgccgaag agctgatgat
     5761 cgccgacatc tctgcgtccg aattcggtta catcgccggt aacgacataa tctgcgcctt
     5821 tcaggttatg cgctttgccg gaaatgccgg attcctgttt taatgcgttc aaattggtgc
     5881 ggttcagtac gttgaagcgg ttggtctgtt gcaggtgcgt taccagaatg gtttttgcct
     5941 ggctgcccaa acggtcttcc ccgtcggaga aaatgccttt ttggaagctg gagcggttgt
     6001 cgaatgttcc gacggaaatc ggggtacgaa caccgtgata ttgcgtattg taggaggcga
     6061 ctttctctac ctcgagactg cgtgaggatt cggtcgcaca gccggtcagt gaaacggcag
     6121 cggcggcaag gacaacggcg gtggaaacgg ttttcataaa atttacccta aggtcaagtt
     6181 aaggaaataa cgggttgtca ttattgtcct tatgtaaatt taagtcaagg tgtttgtctg
     6241 tgcgggacgg atgcgcgcgg aaggtacgga tatttttcaa acggaatcgc ggcgcgggcg
     6301 ggatgcctgc cttttccgga tttagaggta aacgatttcg ccactccgcc ctttgctttc
     6361 ggcacttgcc caccagacaa atgcgggcag cacgtccccg tagcttttgc gttcgctttt
     6421 ggcttcgccc ggatgggatt tgatgcgttg cggggaattg atggggccgg ggacgaggac
     6481 gttggcgcgc aggttgccga agcgttccca ttcgtcggcg gcgactttgc acaggtagtt
     6541 caacgcggct ttggacgcgc cgaagccgcc ccagtaggct ttgggtgttt cgccgtggct
     6601 ttcgccgacg aagatgacgg acgcgtcggg cgactgcttc agcagcggga acagggcgcg
     6661 ggtcagcccc ataggtgcga cggtgttgat gcggtattgg ttgacccatt cggcgacggt
     6721 ttggaaatcc agcggcgaga gggcgtaaaa atagccggcg cagtggacga tgccgtccag
     6781 tttgccttgc gtggcttcgg caatggtggc ggcgaactga ttgaattctt tttcttccgc
     6841 actcatcagg tcgaaacgga tggcgaaggg ttcggggtgt ccggcttcga caatcgcgtc
     6901 atagactttt tccagttttt tctgatgacg ggcaaccaaa atcacagtcg cgcctgctgc
     6961 cgcataggct ttggcaacct gttcgcccag cccttgcgat gcgccggtaa ctaagatggt
     7021 tttgtcggtc agtgtcgcca tactttttcc tttttggtgg tcggtcgggg gtattttagc
     7081 gttttgccgc accttgtaaa gcgtcctgat gccggccggc gttttcagac ggcatcgttc
     7141 aggctttgca ggcaggcttc cgcggaagcg aaacctgatt gtacggcggc ttcgagcgtg
     7201 gcggggtagt atgggtggag gtagtcgcca gcggggaaga tgcggtgccg gtgcagccac
     7261 gacaaatccg gcggcggggc atcggctgcg gttgtggcgc gtttttcggt gatgacgcgc
     7321 acggcttcgg gttcgcccaa atgcggaagg atgcgtttga ggtcggcgtg ggctttgtcc
     7381 gcccacgccc ggtttgcaaa cgcgccgacg cggtcggaaa cgctgatgac ggcggacact
     7441 tcgttttcag gcagtccgag cctgccccgg caaagcagcc attgcaccgt gccgtcggca
     7501 aggccggtca gcggggcggg caggcggacg ggttcggcgt agcgcagata gacggtggtg
     7561 atggcgtgat agcgaaggtt ttgatatgcc gtctgaacgt gttcgggcgt gccttcgggc
     7621 aggagcgcgg cggcgtggta gggcgcggtg gcggggacgg cggcatcgaa agcttcgccg
     7681 ttgacgagca ctttcccgtc cgggaggggg ttcagacggc atacgcgcgt ttcgaggcgg
     7741 atgtccgcgc cgagccgttg aagctccgcc aaggcgggtt cggcgacgat tgcgcccaaa
     7801 tcctgcttgg gtaggagata gtcgctgccg gattttttcg tcagcacgcc gtcggacaaa
     7861 acgttgcaca acacgcgcag gcttgcggtt tccaaaggcg tgttgagcgc accccacacc
     7921 agcggctgcc aaaactgcat cacggcggca cgcggcacgt tccgctgttt cagccattgt
     7981 gcaactgtcg tgtcgggctg tccgaggcgt gcggacttct gcaaatcgga catatcggca
     8041 agcagtttgg ctttgaatgc agtcggtgca cgccgggcaa gcagcacgcc gcccaaaata
     8101 tgcagcggcg cgggcagggg gagggcgcgg aactgcaaac cgccgtgcat atgccagtgc
     8161 agcggtacgc gcaaaaaggc ggcacgggga tccgaaccga tggttttcat caggcgcaac
     8221 acgccccggt atgcgccgag caaaatgtgc tgcccgttgt ccaaaaaacc gaaaccgtcg
     8281 gtatttccgg ccagtgtgcg cgccctgccg cccgcctgcc ggccggcttc aaacagggta
     8341 acgtcggcgt gccgcgccaa ggtgacggcg gcggacagtc ctgcccagcc tgcgccgatg
     8401 acggcgattt tcgggcgcgg atgcggcgtg ttcatcattt attcctccaa tggttgaaac
     8461 cccgtttttc ggggcggcgg gggttgggtt ttcctgaagg gcgggcggta tgcgccttat
     8521 gcccgttccg gcgtgccggg gcgcggtttg aatccgaata accaggtttt cagggcaatg
     8581 cgttttttgc gcggcgaagg gagggcgatt ttgtatttga ggacgttttg cgcgccgtcc
     8641 cggtcgattt cgttcaatag ctcgtaataa accgccgcca taaccagtcc gactttttgg
     8701 gcttttttat cggcagccgg cagcagcgat acggcttcgc ggtaggtttc acgggcgcgt
     8761 ttgatttgga acgccatcaa ttcggcaaaa ttgcccgtcg ggctgcattg caaaatcacg
     8821 cttgcgggga catcaaaccg ctgcatttcc tccatcggca ggtaaatccg tcccctgcgc
     8881 gcgtcttcgc cgacatcgcg gatgatgttg gtcagttgca gcgcaagtcc catcttgtcg
     8941 gcgtattcca gcgttttgcc gtctgaaaac cccaaaatcc gcgcaatcag gcagccgact
     9001 acacctgcaa cacggcggca gtacagtttc aattcttcaa aactgccgta acgcgcccga
     9061 accaaatcca tctgcatccc gtcgattaag gcttccagtt catatttcgg cagcttgaag
     9121 gtttccttaa cctgccgcaa ggcctgattg acggggtgtt ccggcatcgc gccgccgaat
     9181 accttgtcca aatcgccgcg ccaccagttc aatgtcgcct gtgcaacatc ggggttggaa
     9241 cattcgtcaa ccacatcgtc caattcgcgg caaaaagcat ataaaaccgt taccgcatcc
     9301 cgtttttcct gagtcaggaa acggaagccc gacaaaaaac tggagcggct ttcttctgct
     9361 ttttggcggc aatagtcgag tcctttcacg atttatattc ctaatgatgg gcgggaaagg
     9421 cggattttat cggcatttgg cggtagaggg caatttcggc ggcacgaccc aatccttagc
     9481 ggtttgctca actatcggcg caaattctgt taaaatgccg ctttccttcc tttacacacc
     9541 gcaccgacag gcagaattta tggctctttt gcagatttca gaaccgggta tgtccgccgc
     9601 cccgcaccgg caccgtttgg cggcaggcat cgatttgggt acgaccaaca gcttggtcgc
     9661 caccgtccgc agcggcagtg ccgcctgtct gcccgatgcc gaagggcgcg ttaccctgcc
     9721 ttccgtcgtc cgctatctgg aaaacggcgg cattgaagtc ggcaaaaccg ccctgtccgc
     9781 ccaaaaaacc gacccgctga acaccgtcag ctccgccaaa cgccttatcg ggcggacgct
     9841 tgccgatctg catcaaaata cgcattacct gccttaccgt ttcggcgaca atcaacgcgt
     9901 tatcgaactg catacgcggc agggggtgaa aacgcctgtc gaagtgtcgg cggaaatcct
     9961 caaaaccctt aaatcgcgcg ccgaagaaac cttgggcggc gatttggtcg gcgtggtgat
    10021 taccgtccct gcctatttcg atgatgccca acgccaagcc accaaagatg ccgcgcgtct
    10081 ggcgggtttg aacgtattgc gcctgctcaa cgaacccacc gctgccgcaa tcgcctacgg
    10141 gctggacaac gcctcggaag gcacgtttgt cgtgtacgac ttagggggcg gcacattcga
    10201 cgtatccgta ttgcaactga ccaaaggact gtttgaagtc aaagccaccg gcggcaacag
    10261 cgcgttgggc ggcgacgatt tcgaccaccg cctgttctgc cgcctgctcg aacaaaacgg
    10321 actctcccaa ctcaacgaac aagacagcca actcctgctc tcgctcgtcc gcgccgccaa
    10381 agaacaatta accacgcaaa ccgaagcgcg cattcaggcg acgctttcag acggcatggc
    10441 aatcgacaca agcatcagtc gcgccgagtt ccacaacctg acgcagcatt tggtgatgaa
    10501 aacgctcgaa ccggtcacac aggcgttgaa agatgccggt gtcggtaaaa acgaagtcaa
    10561 aggcgtgatt atggtcggcg gttcgacccg tatgctgcac gtccagcagg cagtcgccac
    10621 ctttttcgga caaaccccgc tgaacaacct caatcccgac gaagtcgtcg cgctcggcgc
    10681 cgccatacag gcaaacgtcc tcgccggcaa caaagccgac ggcgaatggc tgctgctgga
    10741 cgttacgccc ttgtcgctcg gtttggaaac ctatggcggg cttgccgaaa aaatcatccc
    10801 gcgcaattcc accatcccca ccgcgcgcgc gcaggacttt accaccttca aagacggtca
    10861 aaccgcgatg acgatacacg tcgtgcaagg cgaacgcgaa ctggttgccg actgccgcag
    10921 ccttgccaaa ttcaccctgc gcggcattcc gcctatggcg gcgggtgcgg cgcgtatccg
    10981 cgtaaccttc caaatcgatg ccgacgggct gttgtccgtt tccgcccaag aacaaagcac
    11041 cggcgtacac gcgcaaatcg aagtcaaacc ttcctacggc ttggacgacg acaccatcac
    11101 ccaaatgctc aaagacagta tgagcaacgc cgccgaagat atggcggccc gcgcccgcgc
    11161 cgaagctgta gtcgaagccg aaagcctgac cgatgccgtc aacgccgccc tcgagttgga
    11221 cagcgatttg ctggatgccg aagaattgca acagattcgg caaggcatcg ccgatttgca
    11281 aggccgtctg aaagacggaa aagccgaaga catccgtgcc gccgccgcca aactcggcag
    11341 catcaccgac aatttcgccg ccaaacgcat gaaccgcaac atccaacgcg cgctgactgg
    11401 tcaaagcgtt gataatattt gacggcaaat ggtttcagac ggcattggat aaagaggccg
    11461 tctgaaaaaa tatcatctca cagaaataaa ataaaaaaca acaatggaaa aagcaggcat
    11521 tgtctatctg atgaaaaccg tcatcaaggg cgtgtataaa atcggcattt cggacgtaag
    11581 caattttgaa ggcagaatgc gccatttgga aaacaacggc tatgcgaacg ttgccggatt
    11641 ggaacgcatc ctcgccgtca aaaccgacaa ttacaaagaa aaagaaaacc tgctccatga
    11701 aattttcagc aaaagcagga taggcgatac cgaattgttc gccgtggacg aaaaccttgt
    11761 aaaacgtttg tttttatcgc ttcgcggcga aatcgtgttt ccgaaaaacg aaacggcgga
    11821 atcggaattt gaaaaaagcg tccacgaacg caggcaggaa gggaatgccg gatccggtcg
    11881 caaacaactg cttgatttgg tacggcgcgg acaccgggaa tacccttacg cgctgccccg
    11941 gcttttggcg ggcgcggcat cctacaagcc gaaaaaatcg aaaatccgcc tttttaaaga
    12001 agcatatttc ggcaaaagcg gcacgaggct gaccgacgaa attgcagacg gcatccatat
    12061 ttacacctgt ttttcgcggg cggatttgga aaaagcctat tccgaatatt tggaactttt
    12121 caaatccgaa tcggatgccg aaggcagaaa gccgcagtaa ggtgcaaaca gataccgtac
    12181 acgttgagga gcagatatga tgggcgattc cgtcatttat tatgtagaac cggtaaaccg
    12241 tgccggcgaa cgcgcccgca aaacattcaa atacttttgg cgcgagcttt tttgggaacg
    12301 ccgccgcatt attcccaccc tggattttgc catggtcaaa gtcccttttt tccaagacgg
    12361 cgaagacggc gaaatttgcg aacatatgtg gatcgacgat atttattttg acggtcttta
    12421 catttatggt gtgctgaaca atgaacccgg cgaactgacc aatgtcgaac aaggcgaaag
    12481 catttgcgtt ccggttgacg acatcagcga ctggatattc gtgtgcaacg gcattcccta
    12541 cggcggcttt accgtacagg caatgcgcgg gcagatgacg gaagaggagc gcaccgaaca
    12601 cgatgccgca tggggaatcg atttcggcga tccctggcag gtattgctgg cgtatgaaga
    12661 aaaagaacat cccgaaaatc tggaagagca tccgatgtgc cggaactgta ttgacgattt
    12721 tcggcaacag ttgtcccaaa atccggattt tctgcatgag caggacgaag acggctatac
    12781 gccgcttcat catgacgcca tcgcaggaaa tgcacttatg gttaaagcta tgcttgaata
    12841 cggcgcaaat cctgcctcaa cgacatcgga aggctatacc gccctcgatt ttgcccgcct
    12901 gacgggctgg caaaatgttg ccgacctgct tgaaccgcga cattaggcgg acagttttcc
    12961 gaaaacgaac acaaacactt ttacagaaag acaataaaaa tgcccaaaat caccgtactt
    13021 ccacacacga cattatgccc cgaaggcgcg gttatcgaag acgcgcccga aggcaaaacc
    13081 gtccttgacg tactgctcga ccacgacatc gaagtcgatc acgcctgcga aaaatcctgc
    13141 gcctgcacca cctgccacgt catcatccgc aaaggtttcg acagtttgga agaaccgacc
    13201 gaattggaag aagacctgct cgaccaggct tggggtttgg aagccgattc gcgcctgagt
    13261 tgtcaggcgg ttgttgccgg cgaggatttg attgtggaaa tccccaaata caccatcaac
    13321 cacgcgcgtg aagaacactg aaaacaggcc gtctgaagcc ggcacgcttc agacggcatt
    13381 gttgcgcaga taaggcgcaa tcgcccgaaa acaggcgttc gtacaggcgg aatcgcgcag
    13441 gcgcgattta cggatattca ccggtttcgc agccggaaaa accgccggcg ggttactttc
    13501 aaggcggcgg tcgggcggta tgccggatag cattccactt tcgcttctat attgattttg
    13561 attggttttc tgacggaatg acccgatgcg gcatccggga cggcttgtgt tttttcctgc
    13621 ccgcctgccg gattttccca tccttgcgtg aaaccgaaag agacggcggc gcggcggaca
    13681 agctcgagat agcgtccttc aagctccggg caggcggcgg acacgctttc tgccgtaacc
    13741 gtgaagccgc cgtccgaccc ggcaaggcgt tccgcaatct gcgtgtagat gcgccaacgt
    13801 tgggcgcgca acattgccgc atcgcaaccg gcagccgctt tatccaaggc aatcaggccg
    13861 cgcctgctcc ggtagtgaag gcaaaccgtt actctggaga gggtatgttg cccgtccaat
    13921 atttgataca gcttgcgtat cagcaaaatc aggcggtcaa actcccccat ctccgacagg
    13981 gggtcgggat ggtcggaagc ggtataaacc agtttggaca attttgcggc aaacatattg
    14041 ttcatcaatc ttccttgtcg gttgaaaccc cgctcttcag ggcggtagaa tcagatttgt
    14101 ttgggagagg cagctcctcc cgaatcagga cgacacataa ggtggtgctt gatgtgttgt
    14161 ccggcgagtt gaaacattca gccatcctca aggtgcggca gttttgccga aacatattct
    14221 acacggcttc aatgccggac gataaaagga aattcatatg aaatggaccg acacccggcg
    14281 tatcgccgaa gaactctatg acctgcacgg cgaaaccatc gatcccagaa ccgtgcgctt
    14341 tacccaactg cgcgacctga ttatggcatt gcccgaattt gatgacgatc ccgcccgttg
    14401 cggcgaacgt attctcgaag ccgtgcagca ggcatggata gacgaggcag aataagtttc
    14461 gggaatgccg tctgaaatac ggcggtacgc ggttcgtgct tctgtttgca gtgggaacgg
    14521 ttttaccagt ctcctttttt cagcctatcc agttggcggc ggtcgcgctt ggtcggtctg
    14581 ccgtcgggat gggcggaagt gatgcggctg aattggtcga gctgtttgcg ctcttccctc
    14641 aatgctgccg tttttgcgtc ttcttcatac agaagccgcg cctcggatgc cgggcggcgt
    14701 tggtggttca aacctttaac cttgatttta tagggaaggg aattgagcgt caggtcgata
    14761 atatcgccga tgtctatggt tttactgttt ttgaccttcg agccgtttac ttgaacccta
    14821 cccagttcga tgtgcttttg cgcaagggaa cgggtcttga aaaaacgtgc cgcccaaagc
    14881 catttgtcca gccgcatggc ggaagaatcg tgcttgtctt tcatacgatt ttgtttgaaa
    14941 taattgaatt tgtttcgagt ttagcataag atacgccgcc tataactagt atatatgcac
    15001 taatccactg ttttccatgc tgtccgaaca caaaaagagg gtgtggaaaa gccgttttgg
    15061 acaataaatt aactgcggaa tatgcacaaa tagcgtatga tagcggcaga atctgttgat
    15121 gagagcttca ttctatgaaa cctgtttttt tggattttga acaacccata gccgaactga
    15181 ccaacaaaat cgatgagctg cgtttcgtcc aagacgagtc tgccgtcgat atttcggacg
    15241 aaatacaccg tttgcagaaa aaaagcaacg acctgaccaa atcgattttc agcaaactca
    15301 cacccgccca aatttcacag gtttcccggc atccgcagcg tccctatact ttggattaca
    15361 ttgaggcact gtttaccgat tttgaagaac tgcacggcga ccgccacttt gccgacgatt
    15421 atgcgattgt cggcggattg gcgcgtttca acggacaaag cgtgatggtc gtcgggcatc
    15481 agaaagggcg cgacaccaaa gaaaaaatcc gccgcaactt cggtatgccc cgtcctgaag
    15541 gctaccgcaa agccctgcgc ctgatgaaga cggcagaaaa attcggcttg cccgtcatga
    15601 cctttatcga tacgccgggc gcgtatcccg gcatcggcgc ggaagaacgc ggacagtcgg
    15661 aagccatcgg caaaaacctg tacgaactga cgcgcctgca cgtccccgtt ttgtgtaccg
    15721 tcatcggcga agggggctcg ggcggtgcgt tggcggtcgc cgtaggcgat tacgtcaata
    15781 tgctgcaata ttcgacctat tctgttatct cccccgaagg ctgcgcttcg attttgtgga
    15841 aaaccgccga aaaggcggcg gatgcggctc aggctttggg cattactgct gaccgcctgc
    15901 aaaagctgga cttggtcgat accgtcatca aagaaccatt gggcggcgcg catcgggatt
    15961 tcgggcaaac catgaaaaac gtaaaagccg ttttggaaaa acaactgcac gaagcgcaaa
    16021 gcatcccgct tgccgatttg ctttcgcgcc gtttcgaccg cattatggct tacggcaaat
    16081 tttcggaaca ataattcagg tagaacaagc agcaagcagt ttgtctgaaa ctgcttgctt
    16141 tttctttatc gggacggaac cgtgctgact ttagatgcgt ttgagcaatg cttgaaggat
    16201 tgttttcctc aaggtctgaa tggaaaaaaa acagcggtgg cattaagcgg cggcttggat
    16261 tccgtcgttt tgctgcatct gcttgtccgc gccgggaaaa aaggcggttt tattccggat
    16321 gcattgcata tccatcacgg cttgagtccc cgtgccgacg attgggcgga tttctgccaa
    16381 aactattgcg atatgctcgg ggtggggctg gaaacggtta agatctgcgt ggaaaaaaac
    16441 ggtttgggca tcgaggcggc ggcgcggcaa aagcgttatg ccgcgtttgc cgaaaaaggc
    16501 tttgacgttt tggcgttggc gcaccacagg gacgatcaaa tcgaaacctt tatgctggcg
    16561 gtcgcgcgcg gcggcggttt gcgcgctttg gcggctatgc ccgccgtccg cccttttggg
    16621 gaaaaaggca ttatctggcg gcccttgctg cctttttcgc gccaagatat atgggattat
    16681 gcccaaaaac acggtttgcc gaatatcgag gacgaaagca atagcgatac ggcttatttg
    16741 cgaaaccgct tccggcaccg tattttgccc gaactttcgg cgcagattcc ccatttcggg
    16801 cggcatctgt tgaacaatgt ccgtgccttg caggaggatt tggctttgtt ggacgaggtc
    16861 gtcgttcaag actgccgttg ggtttgcgga gccggttatt tcgatacggc gcggtggctg
    16921 acgttttccc cgcgccggaa aacccatatt ttgcggcatt ttttgaagga aaacggcatt
    16981 cccgtgccga atcagaatgc ccttgccgac attgcccggg ttttgacgga ggcaaaaacc
    17041 ggacgctgga acgtgcaagg ctttgaattg catcattatg caggcaggct gtttgtgttc
    17101 cgaccggaaa aaacggataa actgcggttt ttgaaagaca ggcagataag cggaaattta
    17161 agggaaatat tggcggggca gggatttgtt ttaaaacgtc atccgttcgg acttcccggg
    17221 catcttttgg agcaggacgg aatcttgcgg acggttgcgg catcggacag gctggctgtc
    17281 ggcggtatcc ataaggatgt gaagaaaatc cttcaggaaa agcgggtttt gcccgcgctg
    17341 cgcccgtttt ggccggtcgt cgccgacaag gacaaccgtc cgctggcgtt ggcaaattac
    17401 ggtacggatt ttcaaatctc ggtttcagac ggcattttgc ccgtccatcc ggattttccc
    17461 gttttatttt gatggaatcg gaatcgccgt tacctattat atattcttga aaaggcggta
    17521 aagtgaagca tatcagttcg gcaaacaatg aacacatcag acacctgcac cgcctgttgt
    17581 ctcaagggaa gttcagacgg caatacgccc aaaccgtttt ggagggcgtg cacctgcttc
    17641 aggttttcct gcaatccggc gggatgccgg tcggggtata tattcccgaa gcgaaaatgc
    17701 cgtctgaaga agtccgtaaa ttgacggcgg ttttgccgga agacggggtt ttttccgttt
    17761 cagacggcat attgaagaaa atcagcagcc tgacttgcgc ggatgatgtg cttgcgctga
    17821 ttgatattcc ggatgcgggt gctttgccgg ccggcggcga ctgtgtggtt ttggacggcg
    17881 tgcaagatcc gggcaatgtc ggcacggtgt tgcggagcgc ggcggcggca ggaatcggcg
    17941 cggtcgtttt gggcaaaggt tgtgcggacg cgtggtcgcc caaagtgctg cgagccggaa
    18001 tgggcgcgca tttcttgtcg gagatttatc cgcaggcgga tttggaaata tggttggcgc
    18061 gttatgaaga ccgtgtgttt gccaccgcgt tgcgtgagga aaaacaggcg gttctgtata
    18121 acgaagattt gcgcgaaccg acagcctggg tgtttggcaa cgaaggcgcg ggtgtcggca
    18181 aagcggtttt agacagggcg gacaagtgcg tcaggatacc gatgcacgat gcaaccgagt
    18241 ctttaaatgt cgcgatggcg gcgacaatct gcctgtttga acagatgcgg cagcggtcgg
    18301 cgtattgagg aaaagaaatg ccgtctgaaa aaatctatta cggcgtattg attttcttat
    18361 gtatcgcttc tatgctgctg tcgccgtttt tttatgcggg tgctttgaag cccaagaagg
    18421 cggcattgcg gaaggacggg cagtggaaac tcatcttatt gtccaatacc gtggcggcgg
    18481 cggttttggc ttgggtgtgg tggaaatggt tttgacagat attgctcaaa atcgtgctaa
    18541 tggaatccga acaaataaag aattttgtaa aaaatttgtt aaatcaatgg attaaagttt
    18601 ttcgggaaac aaaacagctc taagcaaata gggcgtttgt cggtaaatac ggaagagttg
    18661 cggcattatc gggcatcttt aacaagtagt gccggcttgg caggcaattg gtttttatgg
    18721 gcagcttgca aaatcgcgga tataaaattg cgaatcggtt aaagcgtgag gataccatga
    18781 aaaattgcaa atttttttat gatccgacaa gggcaatcta tgatagcggt gcagattatt
    18841 taaccagaga aaaacataga ttagtcgtta tcgcaaatag tgcttggggg ctattgctta
    18901 atttatcttg ttattatgac gaggttttgg aaaagcggaa aataccgttc ggcaaacagg
    18961 aaattgatga cgatatggac aaagtgtccg cccataagcg gaagtttaaa gatatttctg
    19021 aaatcaaagt aggggatggt tgggaatacc cgttcaatta tgagcaggga atgaaagaat
    19081 tagatgaagt gctattgaaa tacattccct tttttgaaga agaacgataa aggaggttga
    19141 tatgcgcgta tctaaaataa ttggaagtat gttgcttgtt acagcggttc agaccgtatt
    19201 ttcggcaaat gtttacgcgt gccgccataa tggtaaaacc agttacagcc aaactccggg
    19261 aaaacattgt accaacgcgg gtttggggcg ggaccgggtg tacagttcgg ttagaccggc
    19321 agtaaaagac agggcggaag acgcaggggt cggcgattat tcggacacgg tgagggacga
    19381 acacgtccaa aatccgaaag agaatatgca taaggatgct tcgaataccg gtgcaaagac
    19441 gcattgattg aaaccgaatc agccatgacg gcgttgaata atagaatttg aacgattagg
    19501 gaactctgat gaaacacatc cacattatcg gtatcggcgg cacgtttatg ggtgggattg
    19561 ccgccattgc caaagaagca gggtttgaag tcagcggttg cgatgcgaag atgtatccgc
    19621 cgatgagcac ccagctcgaa gccttgggca taggcgtaca cgaaggcttc gacgcggcgc
    19681 agttggagga atttaaagcc gacgtttacg tcatcggcaa tgtcgccaag cgcgggatgg
    19741 atgtggttga ggcgattttg aaccgcgggc tgccttatat ttccggtccg caatggctgg
    19801 ctgaaaacgt gctgcaccat cattgggtac tcggtgtggc ggggacgcac ggtaaaacga
    19861 ccaccgcgtc catgctcgca tgggttttgg aatatgccgg cctcgcgccg ggcttcctta
    19921 tcggcggcgt accggaaaac ttcagcgttt ccgcccgcct gccgcaaacg ccgcgccaag
    19981 acccgaacag ccaatcgccg tttttcgtta tcgaagccga cgaatacgac accgcgtttt
    20041 tcgacaaacg ctccaaattc gtgcattacc gtccgcgtac cgccgtgttg aacaatctag
    20101 aattcgacca cgccgacatc tttgccgatt tgggcgcgat acagacccag ttccaccacc
    20161 tcgtgcgcac cgtgccgtct gaaggcttaa tcatctgtaa cggacagcag caaagcttgc
    20221 aggatacttt agacaaaggc tgctggacgc cggtggagaa attcggcacg gaacacggct
    20281 ggcaggccgg cgaagccaat gccgacggct cgttcgacgt gttgctcgac ggcaaaacgg
    20341 ccggacgcgt caaatgggat ttgatgggca ggcacaaccg catgaacgcg ctcgccgtca
    20401 ttgccgccgc acgtcatgcc ggagtcgata ttcagaccgc ctgcgaagcc ttgagcgcgt
    20461 ttaaaaacgt caaacgccgc atggaaatta aaggtacggt aaacggcatc accgtttacg
    20521 acgatttcgc ccaccatccg accgccatcg aaaccaccat cgaaggcctt cgtcagcgcg
    20581 tcggaaacgc ccgcattctc gccgttctcg agccgcgttc caacaccatg aaactcggca
    20641 ccatgaaagc cgccctgccc gaaagcctca aaggtgccga ccaagtgttc tgctacgccg
    20701 gcggcgtgga ctggaacgtc gccgaagccc tcgcgccttt gggcggcaag ctgcacgtcg
    20761 gcaaagattt cgatgctttc attgccgaaa tcgtgaaaca taccgaagca ggcgaccata
    20821 ttttggtgat gagcaacggc ggtttcggcg gaatacacac caaactgctg gacgctttga
    20881 gatagcccgg gcgatgccgt ctgaaagccc ttcagacggc atcgtccggc tgcgcggcac
    20941 aaaggcggaa aaaccgtttg ccccgtattt tcaaacgcgt tacacttgcc gccgctgttt
    21001 tcagccattt gattacccgc aaccgccgtc attgcgccgg cggtttgcct gtcagcgtca
    21061 ttgcgccgct gtaaatacga aagaacacat tatgaccgta tcccccgtcg ccttgcgccg
    21121 taagaccgag tgcaagcctc atcccaccgc gcgctattgg aaaaaatgcg atgtcgaagc
    21181 cctgttcgga cttcccttcc tcgacctcat ttaccaagcc gccgaaatcc accgccaaaa
    21241 tttcaacccg cgcgaaatcc agctttccac gctgttgtcc atcaaaaccg gcggttgtcc
    21301 cgaagattgc gcctactgtc cgcaatcggc gcaccacaac accaatctgg gcaaagagca
    21361 gatgatggat gtggatgaaa tcgtcgaaaa agccaaaatc gccaaatcgc gcggcgcaag
    21421 ccggttttgt atgggcgcgg catggcgcgg ccccaaaccc aaagacgtgg agacggtttc
    21481 cgcaatcatc aaagccgtca agggcttggg tatggaaacc tgtggcacgt tcggtatgct
    21541 cgaagaaggt atggcggaag acttgaaaga ggcgggcttg gattattaca accacaacct
    21601 cgacaccgac cccgaccgct acaacgacat catccataca cgccggcatg aagaccgtat
    21661 ggacaccttg ggcaaagtcc gcaacgccgg tttgaaagtc tgctgcggcg gcatcgtcgg
    21721 gatgaacgaa acccgcgccg aacgcgcagg gctgattgcc agcctcgcca acctcgaccc
    21781 gcagcccgaa agcgtgccga ttaaccggtt ggtcaaagtg gaaggtacgc cgcttgccga
    21841 tgccgaggat ttggactgga cggaatttgt ccgcaccatc gccgtggcgc ggattacgat
    21901 gccgcaaagt tatgtccgcc tctcggcagg gcgcagcaat atgcccgaag caatgcaggc
    21961 gatgtgcttc atggcaggtg cgaactcgat tttttacggc gacaagctgt tgaccacagg
    22021 caatcctgat gaggacggcg acagaatcct gatggaaaag ctcaacctgt atcccttgca
    22081 gtttgaaccg gaaggcgagg tcgccgaagt ggaaaaagcc tctgggatta aagtggatta
    22141 ttgacgattg aaaaatgccg tctgaaaccc ggaaaaaggc tttcagacgg catttgtccg
    22201 gacggcattt ccaatatctt tttaccggcg cgtgatgctg ccgtcgggcg agacatccag
    22261 cccgttcccc ttggggaaat cgctgcggtt cagataaata atccgcccga taacggtttt
    22321 tcagggtcga ttttggggat tttatcgtcg gcttgggtaa tagggatgat agttccggaa
    22381 ataaatttac cctgtttgtc ggtgataatt ttgaaaatcg gcgcaatgcc gctgatgccg
    22441 gaggtgttga ttgcgccgta ggtggcaaag ttgccgccgc tgtaggagat gaagcggtcg
    22501 cggtaaagtt cgacggcgcg agtaacgtgc gacccctgcc cgaatacgac atccgcgccg
    22561 gaatcgacgg caagccgtgc aaactcgacg acgttgcccc tgttttcccc atagaagatt
    22621 tcggtatcga acggcaggtg ttccgcctgt ttcccttccg cgccgccgtg gaacatcaca
    22681 atgacgatgt cggctttctg tttggttttg gtaatccgtt ttctaacttt ggcataatcg
    22741 ttcagtttga cggcggcaag gttgggggcg aaggagacga agccggatct tacgccgttt
    22801 ttcttcagga tggcggtttc aaacctgttt tcgatgcccg aatatttgat gttcaattcg
    22861 tcaaggttcg ccctttatgc cgtttccgca ccgcaaaccg ccggggtcaa gccctcggcg
    22921 catcctgccg gaacggaatc cccgtgctgc cgattgacgg ttcaaacccc gcgcccgttt
    22981 caaataccgg cgatgtggac ggacaggatg cgcctgacga aaagacagcc gataccgttt
    23041 ccattatcgg cgtgggcgac attatgctcg gcagcaatta tccggtcgat tacctgcccg
    23101 ataccaatat tctgaaaaac gtcgaatctg ccttgcaaga cgcggacatt accgtcggca
    23161 acctcgaagg cacgctgttt gacgaaggcg gtacgccgaa aaaatgtgca aacccccaaa
    23221 atatgctatg cattccgaac gccctccgca tacgggcaat accttgccga cgcgggattc
    23281 gactacctca gcttcgccaa caaccacagc aacgacttcg gcgcgcaagg catcacggca
    23341 acggcggcga gcggcagctc ttttacatac tcgatcgcgc taaagccgct gccgataacg
    23401 aggccaaaat tgcggaaaat accgccatcg cccagataaa tttgtccatc atcagacctt
    23461 tactgttcag acgagacagc atttgccgca cgttttgggg cttatctttc gatttgcgct
    23521 acgtcgcgca ccgcgccttt gtcggcggaa gtcgccatcg cgccgtaagc tcttaatgcg
    23581 gcggagacgt agcggtcgcg gtttttaggc ttccatgctt tgctgccgcg cgcttccatt
    23641 tcggcacggc gtgcggcaag ctcttcgtcg gaaatggcaa ggtggatgct gcggttgggg
    23701 atgtcgattt cgacggtatc gccttcgtgt accaaaccga tcgcgccacc ttccgccgct
    23761 tcgggcgagg cgtgtccgat ggacaaacct gatgtgccgc cggagaagcg tccgtcggtt
    23821 aagagagcgc aggctttgcc gaggccttta gatttcaggt aggaagtcgg atacagcatt
    23881 tcctgcatgc ccgggccgcc tttcgggcct tcgtagcgga tgatgacgat gtcgccggcg
    23941 acgatttggt tgcccaaaat gccttctact gcgtcttctt ggctttcaaa cacgcgggcg
    24001 cggccggtga atttgaggat gctctcatcc acgcctgcgg tttttaccac gcagccgcgt
    24061 tcggcgatgt tgccgaacaa gaccgccaaa ccgccgtctt gcgagtaggc gtgtgccacg
    24121 tcgcggatac agcctttttc gcggtcgagg tcgagggttt tccacatacg gttttgcgag
    24181 aacgcttggg tggtgcgtac gccgccgggc gcggctttga agcgttcgat ggcgcgggtg
    24241 ttttcagggt tggttacgtc ccatttttca atcgcgtctt tcagggtcag cgcgtgaatg
    24301 gtgtacacgt cggtgtgcag tttgcccgct ttgtccaatt ctttcaggat ggcgaagatg
    24361 ccgccggctc ggtgcacgtc ttccatatag tagtcgtggt tgttgggcgc ggttttgcag
    24421 atgcaaggca caacgcggct caggcggtcg atgtccgcca ttttgaaatc gacaccggct
    24481 tcgttggcaa cggccaacag gtgcaaaatg gtattggtag agccgcccat ggcgatgtcc
    24541 atggtcatgg cgttttcaaa cgcttttttg gtggcgatgc tgcgcggcag tacggtttcg
    24601 tcgtcttgct cgtaatagcg tttggtgatt tcgacaatca tacggcctgc ttcgaggaac
    24661 aattctttgc ggccggcgtg ggtcgccaaa tacgaaccgt tgccgggcag ggaaaggccg
    24721 agtgcttcgg tcaggcagtt catcgagttt gccgtaaaca tacccgaaca cgagccgcag
    24781 gtcgggcagg cgttttgttc gacttcctcg acttgccggt tgctgacatt gtcgtccgcc
    24841 gattcgatca tcgcgtcaat caagtccaaa cggcgttcgg gttggatgtt tgccacgccg
    24901 ataactttgc ccgcttccat cgggccgccg gagacgaaga tggtggggat gttcaggcgc
    24961 atcgcggcaa tcagcatgcc cggggtgatt ttgtcgcagt tggaaatgca caccagcgcg
    25021 tcggcgcagt gggcgttgac catatattcg atggagtcgg caatcaaatc gcggctgggc
    25081 agggagtaca gcatgccgct gtgtcccata gcgatgccgt cgtcgatggc gatggtgttg
    25141 aattctttgg cgattgcgcc ggctttttcg atttcgcggg caaccagctg gcccatattg
    25201 tgcaggtgga catggccggg cacgaattgg gtgaaggagt tggcaacggc gatgatgggc
    25261 ttgccgaagt cggtttccat cacgccggtg gcgcgccaca atgcacgtgc gcccgccata
    25321 ttgcggccgt gggtggaggt tttggagcgg tattctggca tagtgtgttt ccttgtgcct
    25381 ataccgtctg aaagacaggg ctgtttcaga cggtatcggg tacggttttt tagagtggga
    25441 aaagagggta ttttatacca agtatcggaa ttttgcggga ttgaaacggc gtgcggcaaa
    25501 aaagaaaatc cccgcaggaa tgcggggacg ggttcaggcg cgggcaatcg cgacggcttt
    25561 ggatgcgtcc caaaaatcaa cgggggcatt taatacgggt ttgacgatgc ccgtccgtat
    25621 ggcgaaatcg ccgaaacctt cgccgatatt gcgttctgcc gcccatttgc cgatcaggtc
    25681 gtccaattcg gcaaggattt cgggcagggt gatgttttct ttgtaaaggc gggggatgcg
    25741 tacgccttca cggtcgccgc cgatatggag gttgtagcgt ccgacggctt tgccgaccag
    25801 cccgatttcc gccaacattg cccgtccgca gccgttgggg cagccggtaa tgcgggtaac
    25861 gatgtagtcg tccgacgtgc cgtgtttcgc cataatctta tccagatcgt cgatgaagtc
    25921 cggcagcacg cgttcggctt ccgccattgc caaggggcag gtcggaaagg aaacgcagga
    25981 catcgcattt tcgcgcagct tgctgacatc gttgcggatt aatccgtatg ttcgggcaag
    26041 ttcttcgatt tttgccttgt ctgcttcggc aacatttgcc acgataaggt tttggtttgc
    26101 ggtaatgcgg aaatcgcctt tgtggatttt ggcgatttcc aacacgccgg tcagaagctg
    26161 tttcccgcct tcgtcaacca aacgcccgct ttcgatgaaa agggttaaat gccagttgcc
    26221 gtctatgcct ttcacccagc cgatgcggtc gccgcgcccg gtaaatttga acgggcgtac
    26281 gggttcgaac ggcataccca tacggcgttc gacttccgcg cggaagttgt ccaagcccat
    26341 attttgaatg gtgtagcggg tgcgggcgtt tttgcggtcg ctgcggttgc cgaagtcgcg
    26401 ctgcgtggtt accaccgctt cggcggcctt cagcgcgtgt tccggaggca cgaaacccag
    26461 ttccagtgaa atgttcggat aggttttggt gttgccgtgt tccatcgaaa gcccgccgcc
    26521 tgccaaaaca ttgaagccgg caagctgtcc gttaccgtct gaaacggcga cgaaatccaa
    26581 atcgttgccg tagcagtcca catcgttcaa gggcgggatg acgactgcgg ttttgaattt
    26641 tcgcggcaga taggttttgc ccaaaatcgg ctcgtcttct tgaaggaagt cgtcggaact
    26701 ttgaactttt ttgccgtcca cccacacatc cagataaccg cgcgtgcgcg gcagcaggtg
    26761 ttcggaaatc tttttcgcgt attcgtaagc ctgccggtgc agttcggact cgatcgggtt
    26821 ggacgtgcaa agcacgttgc ggttcatatc cgccgccgtg gcgatggaat ccaaacccag
    26881 tttgtgcaag aggcggtgca tcgtctgcaa cttggctttc ggcacgccgt gaaattggaa
    26941 ggtttgccgg ttggtcagcc ggatggagcg gtaatgactg ttttcccggg caaatttgtc
    27001 cagttctatc cattgggacg gtttaatgat cccgcccggc agccggcagc gcaaaagcat
    27061 aaatttcaag ggctcgagtt ttgcctcggc gcgttcggcg cggatgtcgc ggtcgtcctg
    27121 ctcatacata ccgtggaagc ggatgagttg gaagttgtcg cctttgaagc cgcccgtgag
    27181 cgggtctttc aaatcgtcca aaatcgtgcc gcgtaaaaaa ttgctttcgg ttttcagacg
    27241 ttcgttgtcg gatagcggtt tttcttgcca cgccaaacct tttgtcttgg tctgtacggt
    27301 cattttgtgt tcctcccgat tatatttaat caataaacat cacgctgata gcgtttttct
    27361 tcgcgcagca tatccaaata ttcttctgcg ccctcttcgt ccaaatgtcc tgccccgata
    27421 atcacatcca gcaaggcggc ttccacgtct tttgccattt ttgccgcatc gccgcacaca
    27481 tagatatgcg cgccttcctg cagccattgc caaagtcctt ccgcctgttc gcggattttg
    27541 tcctgcacat agattttttc ttcctgatcg cgggaccagg cgaaatcgta cctgtgcagg
    27601 aagccgtctt tggcaaactg ctgccattcg gtctgataga ggaagtcggc ggcaaaatgc
    27661 ggattgccga aaatcagcca gtttttgcct tccgcatttt ctgcggcacg ttgttggacg
    27721 aaagcgcgga acggtgcgac gccggtgccc gagccgatca tcacaatcgg cttgcggctg
    27781 tcttcgggca gcctgaagcc gtcgttgcgt tccacaaaca cgcgcaccgt gccgtcctct
    27841 tccagccggt cggcaaggaa acccgatgcg ccgcccaccc tggcgcgccc ttcgtgttca
    27901 aaacgcaccg cgccgacggt caggtgcact tcgtccccca cttccgcctg cgacgaggaa
    27961 atcgaataca ggcgcggcgc aagcgggcga agcaggccgg cgaattgttc cgccgtcagt
    28021 tttgccggga agcggtgcag cacatcggca atcggcgtgc tttgcacaaa gccttgcaaa
    28081 acggcgttgt cggcagcaat acggtcgagt tcgtcatcat cggcgaacgg ggcatagcct
    28141 ttgacaaagg cgggggtgtt ttgcgtgagt tcaaaatggg ataacagtgc ggaggcaacc
    28201 ggcagggttt ttccgcccgc ctgtatttcc gttgccggat cgatgccgag caggtctagg
    28261 atttccctga ccagtgccgg atcgttgtca aaccaaacgc cgagcgcgtc gcccgggagg
    28321 tagtgcaaat ccgaaccgct caaatcgatt tcgatgtggc gcacgtcttt atcggattgg
    28381 cgggcggtga ttttctgatt ggccagcagg gcggcgggaa aggggtctgc cttgcagtac
    28441 ctgccatccg gtgccgtctg aaggccggcg gggggcgttg tctgcggcgc gggcgttgcc
    28501 cggttttttg cggcttcttc ttttaagcgc gcgacaatgc ggcctgtcca tccgtctgcg
    28561 gaggcggtaa agtccaaatc cgcatcaacg cgttcgagca ggcgtttcgc ccccaattct
    28621 tcaaaacgtt tgtcgaaatc tttgcccgcc cggcagaaat tcggatagga gctgtcgccc
    28681 aaacccagta cggcaaattg gagtttgtcc aatttcgggg cttttttgcc gttcagcagt
    28741 ttgtgcagca cgacggcttc ttccggcggt tcgccttcgc cttgggtgga ggtaaccagc
    28801 agcaggcggc gttcgccgga gatgtttttc gccttatagt ctttcagttc ggcgcgcctg
    28861 acttggatgc cggcggcttc caggctgtcc gccgctttgt cggcaacgga tttcgcattg
    28921 ccggtttgcg aggcggaaag gacggttacg gaaaaaggtt ctgccgccgg caatgccgtc
    28981 tgaagcgcgg gcagtcctgc agatgccccg tttcctgctt ttgcccaagc gtagccggac
    29041 agccacgccc attgtgccgc gtccagcacc gacaggagct gcgtgatttc gggcggcaga
    29101 ggcggtaatg gcggatttgt gttctgcata tcgtgttcac tcataaaatc atacctgccg
    29161 caacagtgcc gtatgtcgct tcgtctatca ggataaacga accggcggcg gtgttttccg
    29221 cataaggcgt tgccgtaacg ggtttttgaa ggttgatgcg gactttggca atgtcgttca
    29281 tcttcaagga ttccgcgccg gcctcttgtt ccagcgtgcg gacatctaaa acgctttcaa
    29341 tttccccgac ttttgccggc acggtttgcg tgccgtgctt gagcaggtat ttgcgcgcgg
    29401 tgttgagcgg acgttcgtca aaccagcaaa gcgtggcttc cagatgtttt tgcggggcga
    29461 gcggggaatt tttatcgaca aaaaggtcgc cgcgcgaaac atcgatgtcg cggtccagcc
    29521 ggagggttgc cgcctcgccg gcaaaagcct gcgccacttc gcctttgggt gtgatgattt
    29581 cagacacttt ggcggtcagc ccgttcggtt cgatgcggac ggtttgcccg acggcgaccg
    29641 aaccgcgttc gatgcgcccc tgatagcctc ggaaatcatc ggccttgtcg gcatcttggc
    29701 ggacgaccag ttgcacgggg aaataaaaat cgtcggcggt gcggctgact tcgtccgccc
    29761 ccggcagggt ttccaaaatg gacaataagg gttcgccttt ataccaaggc atattgccgc
    29821 cggggtaaac aatgttgtcg cccaagagtg cggacaccgg tacgaaatgc acgtctttca
    29881 aaccgagccg tccggcgagt cggcggtatg cctccacaat ggcgttgaat ttgtcttcgc
    29941 tgtaatccag caggtccatt ttgttgaccg ccaccacaat atgcgggcag tcgagttggc
    30001 ggaggatggc ggaatggcgt ttggtctgcg gcagaagctg caagggctgt gcgccgaaat
    30061 ccagttggga tgcgtcaacc agcacgactg ccgccgaagc ggtgcttgcg cccgtaacca
    30121 tattgcgcgt gtattgttcg tgccccggcg tgtcggcgat gatgaatttc cgtttcgccg
    30181 tggaaaaata gcggtatgcc acatcgatcg taatgccctg ttcgcgttcg gcttccagtc
    30241 cgtcggtcag gatggagaag tctatggttt ctttcaaacc tttgcttttg ccggattcca
    30301 aggttttgat ttggtcggac agcagggctt tgctgtcgta gagcagccgt ccgatcaggg
    30361 tgcttttgcc gtcatcgacg ctgccggcgg taatgaagcg gagcggggcc tggtgttgtg
    30421 ccgtcatatt ttcttcctca tatctgctta aagggttttt gaaatttaga aatagccttc
    30481 ttttttgcgt ttttccattg ccgcctcgct tgcctgatcg tccagccggg tcgcgctgcg
    30541 ttcggaaatg tcggcaaccg ctgtttctct gataatctcc gtcggcgtgg acgcggtgct
    30601 ttctaccggg caggtgcagc tgatgtcgcc gacggtgcgg aagcggacat caaggatttc
    30661 ggaggtttca gacggcattt tcggggtgag cggcgttacg gggaccagca gccccctgcg
    30721 tctgaccact tcgcgcctgt ggctgtaata aatcggcggc agctcgaggt tttcgcgggc
    30781 gatgtattgc cagatgtcga gttccgtcca gttggaaatc gggaagacgc gcatattttc
    30841 gcctttgtgc agcctggtgt tgtacagcga ccacagctcg gggcgttgcg ccttcggatc
    30901 ccattgtccg aactcgtcgc ggaacgagaa aatccgttct ttggcgcggg ctttttcttc
    30961 gtcgcgccgc gcgccgccca taagcgcgtc gaagccgttt gcctcgatgg tttccaacaa
    31021 ggtaaccgcc tgtgccgcat tgcgcgaatc ggtttctttg cgtaagacca ccgtgccttt
    31081 ggcaatggag tcttccacgc gccccactat caggcgggca ttgagttttg ccgcctgcgc
    31141 gtcgcggaaa gcaatcactt cggggtagtt gtgccccgtg tcgatatgca ccagcgggaa
    31201 gggcagtttt accggcctgc tgcccagccg gaaggctttg caggcgaggg cgagcaggac
    31261 cacggaatct ttgccgctgg aaaagagcag ggcggggttt tcgcattccg ccgccacttc
    31321 gcggataatg tggatggatt cggattccaa ccagtcgagt tgggcgttgt tcggttcggt
    31381 tttcgtcata ccatattcct tatttcttct gtctgatatt tatgaattat ttgtgcagcc
    31441 cgcattcttt gctgtttctg ccttcccacc accaccgccc ggcgcggatg tcttcgcccg
    31501 ccttgacggg gcgggtgcag gggtcgcagc ctatgctggg aaatccttgc cggtacaaat
    31561 cgttgtaagg cacattgttg gcgaggatgt atgcccacac gtcgtgttcc gaccagtcga
    31621 aaatcgggtt gtatttgccg atgccccgtc cggcatcgta ttcggcaaac ggcagttccg
    31681 tgcgtgtggc ggattgttcg cggcgttgcc cggtaagcca ggcgtccgcg cctgcaatgg
    31741 cgcggttgag cggttcgatt tttcggatgc ggcagcattc gcggcgcgct tcaacgctgt
    31801 cgtaaaaggc aaacctgcct ttgctttcca cataacggtc ggcatcttct cgaaccggcc
    31861 ggaaacgctt tatccgcaaa tggggatatg cgcgtccgag cctgtccagc aggtttaggg
    31921 tttccgtgtg gagcagcccc gtatccaagg taaaaatgcc gatattgagg ttttcgccgg
    31981 cgataaggtc ggtaatcacc atatcttctg ccgcaaggct gctggcaaac cgtgcatccc
    32041 ggtggctgcc ggcaatccgg tgcaggcgtt gtttgagggt ttccgttttt tccgcaaggg
    32101 cggtttcgcc gccggatccg atatgcggta tctgccacag ggcgggtttg aacagtgtcg
    32161 tttccatttt tcccgcctta tgccgcccgt tgtccggcat tcagtccgcc caatgcggga
    32221 tacgcctgcc cgacccggtt ttctccttcg ccgttttcac cgaaccaggc gagtttttcg
    32281 tgcagcccca ccacttcgcc tatgacaatc aatgccggat tcggcgcggt ttcggcgagt
    32341 tcggcaaggt tggcgagcgt gccggttgcg gttttttgag ccggcagcgt gccttggctg
    32401 atgacggctg ccggcgtgtc gggcgagcgt ccgtgctgtt gcagccgttc ggcaatcagg
    32461 gcggctttga gcgcacccat ataaatcacc aaggtctggc ggctgcgggc gagggtctgc
    32521 cattcgatgt cgggcgcatc cgccttgcgg tggccggtta cgaaaaccgc actttgggca
    32581 taatcgcggt gcgtgagcgg gatgccggca taggcggtcg cgccgacggc ggcggtaatg
    32641 ccggggacga ccgaaaacgg aatctgatgg cgtgccaagg tttccaattc ttcgccgccg
    32701 cgtccgaaca cgaaagggtc tccgcctttc aaacgcacca cgcgcctgcc ttcgcgggcc
    32761 agcctgacca taagcgcatt ggtgtcctct tgcggggtgc ggtcgccccg ggcgcgcttg
    32821 ccgacaaaaa tccgttccgc atcgcggcgg acgagggaca gtatgccgtc tgaaaccagc
    32881 gcgtcgtaaa gcaccacgtc tgcctgctgg atttcctgta gccctttgag cgtcagcagc
    32941 cccgcatcgc cgggacccgc gccgaccagc gagacggagc cgccttgatc attttgacga
    33001 ctttgttcca attggcctgc caattcccgt tcggcaaggg tgttttgccg gtttttgacg
    33061 agggcggcga aacgtccgtt aaactgcttt tcccaaaagc ggcggcgttc ggtaacggat
    33121 ttcagtttgt ccttgacggc atcgcgccac cttcctgaaa tttccgccat atcgcccaaa
    33181 gacggcggca gcagggcttc cagcctttca cgcagcagtc gggcgaggac gggcgcgctg
    33241 ccggagctgg aaacggcaat ctgaaccggg ttgcggtcga taaccgacgg gaagatgaag
    33301 ctgcaatggt cgcggtcgtc caccacgttg accggctttt ggcagctttc ggcaagatgg
    33361 aaaacgcgcc ggttgagggc ttggtcgctg cttgccgcaa tgatgaggaa aactgtgcgg
    33421 atgtgttcgg cacgaaattc ttcggcaagc cacaggattt tgttttccgc cgccaacgcg
    33481 gagagttcgg cattcaggtg ttttgcggca accctgacct ctgcgcccgc cttcagcagc
    33541 aggctgattt tgcgtgcggc gaccgcgccg ccgcctacga ccaatacggg gcggccggcg
    33601 aggttggcga aaatagggaa ataattcact ggctgactcc tttgctgttt gcccgcacct
    33661 tgtttccgat acggtgcgtc gcggcatttt tgtcggaatg cgggtcattt tagacaaaag
    33721 gattttcccg gttaaataat aaaaaggcat ttgttagaag ctgaaagcta tatgagggcg
    33781 gctgcggatg cggcggttt
//
LOCUS       DAXHSF010000004        29935 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.004, whole genome shotgun sequence.
ACCESSION   DAXHSF010000004 DAXHSF010000000
VERSION     DAXHSF010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 29935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29935
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.004"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(1..1183)
                     /locus_tag="ACFM8S_000132"
                     /pseudo
     CDS             complement(1..1183)
                     /locus_tag="ACFM8S_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678130.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            1329..2033
                     /locus_tag="ACFM8S_000133"
     CDS             1329..2033
                     /locus_tag="ACFM8S_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248833.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HFC6313278.1"
                     /translation="MLRSILAASLLAVSFPTAAEALNYNIVEFSESAGVEAVQDTMSA
                     RFQVTAEGRDKNAVNAEFVKKFNNFTRKSKNGSFKTELVSRSAMPRYQYTNGRRIQTG
                     WEERAEFKVEGRDFDELNRFIADIQADAALEYTDFHVSRERRNEVIDQVSKDAVLRFK
                     ARAEKLAGVLGASGYKIVKLNLGHIGSHIAGGGAAQAKMLRAMPMAASVNMEGADSAA
                     QGVEEISISVNGTVQF"
     gene            complement(2098..2664)
                     /gene="dcd"
                     /locus_tag="ACFM8S_000134"
     CDS             complement(2098..2664)
                     /gene="dcd"
                     /locus_tag="ACFM8S_000134"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFC6313279.1"
                     /translation="MSIKSDKWIRRMSEEFGMIDPFEPNQIKEADGKRIISYGTSSYG
                     YDIRCANEFKIFTNINSTIVDPKNFDPKNFVTVEDDCCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGVAQVLFF
                     ESDEICETSYKDRNGKYMGQTGVTLPKA"
     gene            complement(2730..3146)
                     /locus_tag="ACFM8S_000135"
     CDS             complement(2730..3146)
                     /locus_tag="ACFM8S_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MORN repeat-containing protein"
                     /protein_id="HFC6313280.1"
                     /translation="MLKHLAFLLPAMMFALPAASAVLTSYQEPGCTYEGDVGKDGKPA
                     GKGTWRCQDGRNYTGSFKNGKFDGQGVYTVAANREIFIEPFNSDSTKFRNMVLSGTFK
                     KGLAHGRFTVSQNGETLFIMKCENGMIKEVKLPKNK"
     gene            complement(3198..4097)
                     /locus_tag="ACFM8S_000136"
     CDS             complement(3198..4097)
                     /locus_tag="ACFM8S_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFC6313281.1"
                     /translation="MWFKQISFYPLNKEKLPEADVLADKLAEAEFTHCQGLDWFSEGF
                     TAPVSFSPELVFPADFTLRVALKKEEKVLPAGVIRDILEEKVAEIQNNEARNVGRKEK
                     QELKEQITDDLLPRAFTRSSRTEAVFNTRHGYLLVNNAASAKAENILTKLREALGGLE
                     ASLPNTKQSPSSLMTGWLLQGHCEGGFELDSDCELKGTGDIVPVVKVSKQDLTADEVV
                     QHVKNGKTVTQLGLVWREQIAFILTQDFTLKRIQYLDVLQEEAESNGDDAAGLAFASQ
                     ILMAESVSIMLEELVSYLGGWQD"
     gene            complement(4128..5585)
                     /gene="der"
                     /locus_tag="ACFM8S_000137"
     CDS             complement(4128..5585)
                     /gene="der"
                     /locus_tag="ACFM8S_000137"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFC6313282.1"
                     /translation="MKPTIALVGRPNVGKSTLFNRLTRTKDALVHDLPGLTRDRHYGH
                     GKVGSKPYLVIDTGGFEPVVDSGILHEMAKQTLQAVDEADAVVFLVDGRTGLTPQDKI
                     IADRLRQSPRPVYLAVNKGEGGNRAVLAAEFYELALGDPYVISGAHGDGVYYLIEDIL
                     ETFPEPEKEEEEAKHPVFAVIGRPNVGKSTLVNAILGEERVIAFDMAGTTRDSIHIDF
                     EREGKPFTIIDTAGVRRRGKVDEAVEKFSVIKAMQAVEAANVAVLVLDAQQDIADQDA
                     TIAGFALEAGRALVVAVNKWDGISEERREQVKRDINRKLYFLDFAKFHFISALKERGI
                     DGLFDSIQAAYNAAMIKMPTPKITRVLQSAIERQQPPRAGLVRPKMRYAHQGGMNPPV
                     IVVHGNSLHAISDSYTRYLTQTFRKAFNLQGTPLRIQYNVSENPYENADDKPKKKPLR
                     RVSLSNRIEKREGRKEEKNRFKKKTKVSVKKQFSK"
     gene            complement(5736..6365)
                     /locus_tag="ACFM8S_000138"
     CDS             complement(5736..6365)
                     /locus_tag="ACFM8S_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HFC6313283.1"
                     /translation="MAAHLEEQQELDNFKYFWKTTGKWLFAVLILAALGYLGYTVYQN
                     RAASQNQEAAAVLANIVEKAQNKAPQSEINAELTKLQQSYPHSISAAQATLMAAATEF
                     DAQRYDVAEGHLKWVLSNQKDSLIQALAAQRLGVVLLQQKKYDAALAALDTPVEADFA
                     PLLMETKGDVYAAQGKSQEALKNYGQALEKMPQDSVGRELVQMKLDSLK"
     gene            complement(6366..7661)
                     /gene="hisS"
                     /locus_tag="ACFM8S_000139"
     CDS             complement(6366..7661)
                     /gene="hisS"
                     /locus_tag="ACFM8S_000139"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFC6313284.1"
                     /translation="MAQKIQSVKGMNDLLPVEQKDFKLTAAFWQAFEDTVNRWTRAYG
                     YQQIRTPIVEQTGLFVRSIGEETDVVGKEMYTFSDSNDSLSLSLRPEGTASCLRAVVE
                     HNLLYNSPQKLWYMGPMFRRERPQKGRYRQFHQVGIEALGFEGPDIDAEIIAMSADLW
                     EKLGIREYLTLEINSLGNREERAAHRAALVEYLTRYEDKLDEDSKRRLKTNPLRVLDT
                     KNPDLQEICNAAPRLVDYLGEASQNHYARFKAMLDGLGIQYIENPRLVRGLDYYNQTV
                     FEWTTDKLGAQATVCGGGRYDGLIEELGGKPAPSIGFAMGIERLLLLVSEYGSLEVNA
                     APDVYAMHQGKGADLQVMKYAQALRAQDFNVMQYSGYQSLKAQMKKADNSGARFALIV
                     AQDELANGTVTLKDMNGAHGQQTVAAEDLTHTLQQWKNA"
     gene            complement(7787..8443)
                     /locus_tag="ACFM8S_000140"
     CDS             complement(7787..8443)
                     /locus_tag="ACFM8S_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFC6313285.1"
                     /translation="MEQKRRFAASLLLAAVLPLCAHSFPFAEENPIAYGKVKIQSWKE
                     RRDFNIVKQDLDFSCGAASVATLLNNFYGQTLTEEEVLKKLDKEQMRASFEDMRRIMP
                     DLGFEAKGYALSFEQLAQLKIPVIVYLKYRKDDHFSVLRGIDGNTVLLADPSLGHVSM
                     SRAQFLDAWQTREGNLAGKILAVVPKKAETISNKLFFTHHPKRQTEFAVRQIRQARAE
                     "
     gene            complement(8499..8723)
                     /locus_tag="ACFM8S_000141"
     CDS             complement(8499..8723)
                     /locus_tag="ACFM8S_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313286.1"
                     /translation="MMKNKYILTKAAISITAISIFYLLTQESSGKTEEPSYFLMFMFL
                     NLLWFEENKTVMAAVTAMIAAHFIFVALSD"
     gene            complement(8994..9281)
                     /locus_tag="ACFM8S_000142"
     CDS             complement(8994..9281)
                     /locus_tag="ACFM8S_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313287.1"
                     /translation="MDDLIAAFLGYLSGKYSEGKISRLEISLTAGSIFFAVFFIHELI
                     IFIVDGNKENLWNFITEMMKDLFLFSCSVCLLFYFLLFIAETIGKTINKIK"
     gene            complement(9516..9788)
                     /locus_tag="ACFM8S_000143"
     CDS             complement(9516..9788)
                     /locus_tag="ACFM8S_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313288.1"
                     /translation="MFAVIFFSTLGCILAWIRDIPKIKSKKILARSLYIIGIINIIIS
                     YVLIKNILVSVSDEGGIKYVAIYLSNLFFWTVLMYVLVKRLSKKPS"
     gene            complement(10350..10823)
                     /locus_tag="ACFM8S_000144"
     CDS             complement(10350..10823)
                     /locus_tag="ACFM8S_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313289.1"
                     /translation="MKKQITAAVMMLSMIAPAMANGLDNQTFENQVFHTRADAPMQLA
                     ELSQKEMKETEGAFLPIVAGALMGGAISAWANHGISKIKTGQFASTRSTLAATAGGMI
                     GGGYSSAMLRGAGITTSVFARSAWKGGNAIPNAVIRANGAALNQSSSAAMGKHRR"
     gene            complement(10866..11735)
                     /locus_tag="ACFM8S_000145"
     CDS             complement(10866..11735)
                     /locus_tag="ACFM8S_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HFC6313290.1"
                     /translation="MKRIFLSALPAILPLSAYADLPLTIEDIITDKGKWKLETSLTYL
                     NSENSRAALAAPVYIQTGATSFIPIPTEIQENGSNTDMLVGTLGLRYGLTGNTDIYGS
                     GSYLWHEERKLDGNSKTRNKRMSDVSLGISHTFLKDDKNPALISFLESTVYEKSRNKA
                     SSGKSWLIGATTYKAIDPIVLSLTAAYRINGSKTLSDGIRYKSGNYLLLNPNISFAAN
                     DRISLTGGIQWLGRQPDRTDGKRESSRNTSTYAHFGAGFGFTKTTALNASARFNVSGQ
                     SSSELKFGVQHTF"
     gene            11900..12823
                     /locus_tag="ACFM8S_000146"
     CDS             11900..12823
                     /locus_tag="ACFM8S_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFC6313291.1"
                     /translation="MKKRNNPLPLLNGVKPSYLVLPHEKQFYGLPLLHFLCIRFPFVG
                     ADDWRRRLNSGFVVGSDGAALDEHSLFEPGKVMFYYRETSRESEPRIPFEEKILHIDE
                     HLIVVDKPHFLPVIPSGRFLRETLLTRLRLRPELQHLNVEDITPLHRLDKDTAGVMLL
                     SHNPATRGAYQTMFQNKTVWKTYEALAPTRTDLPYPLDVVSRLVRGEKFFTTQEAEGE
                     PNAHTTVELIENRGEFSLYRLTPHTGKKHQLRVHMMGLGMPLLNDALYPVPSEAGSED
                     YRKPLKLLAKKIAFADPLSGRERVFCSRFCL"
     gene            complement(12901..13542)
                     /locus_tag="ACFM8S_000147"
     CDS             complement(12901..13542)
                     /locus_tag="ACFM8S_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFC6313292.1"
                     /translation="MLKPETIHIPGPVGILETIHIPSEQVPARGVAVINHPNPLQGGT
                     NTNKVIQTAAKALSKLGFHCYLPNLRGVGNSEGTHDYGRGETQDCLAVIDYARAQHPE
                     APEFALSGFSFGGYVATFAAQARIPDLLLLIGAAVCHYTGRPEPSAVPNVAKTLMIHG
                     AEDEVVEIEKALKWAEPQDLPVITIAGSTHFFHGKLIVLRDTILRFAPVCLNG"
     gene            complement(13597..14388)
                     /locus_tag="ACFM8S_000148"
     CDS             complement(13597..14388)
                     /locus_tag="ACFM8S_000148"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFC6313293.1"
                     /translation="MARHPYRRLRPAKSCFPEVGISEEGNIRSLHLGSNTVQSSMNLD
                     RPSELVLSYSRAMMGWLLFAERLPQHITQIGLGGGSFARWIDTYLPDTRQTAVDINPQ
                     VIAIARSLFELPFEGEKFEIIEADGAEYIKVFRHNTDVILVDGFDGEQIIDTLVEEPF
                     FRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLSVFEGRVLELPAESHGNVAVMAFQS
                     SPKEQNIDKLKKRADKLSSAYGLDFHRMLAGLKASNPNNGKHFHL"
     gene            14554..14629
                     /locus_tag="ACFM8S_000149"
     tRNA            14554..14629
                     /locus_tag="ACFM8S_000149"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14587..14589,aa:Thr,seq:tgt)
     gene            14664..14739
                     /locus_tag="ACFM8S_000150"
     tRNA            14664..14739
                     /locus_tag="ACFM8S_000150"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14697..14699,aa:Thr,seq:tgt)
     gene            14881..15672
                     /gene="panB"
                     /locus_tag="ACFM8S_000151"
     CDS             14881..15672
                     /gene="panB"
                     /locus_tag="ACFM8S_000151"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFC6313294.1"
                     /translation="MITVNTLQKMKAAGEKIAMLTAYESSFAALMDDAGVEMLLVGDS
                     LGMAVQGRKSTLPVSLRDMCYHTECVARSAKNAMIVSDLPFGAYQQSKEQAFAAAAEL
                     MAAGAHMVKLEGGVWMAETTEFLQMRGIPVCAHIGLTPQSVFAFGGYKVQGRGSKAQA
                     LLNDAKAHDDAGAAVVLMECVPAELAKKVTETVSCPTIGIGAGVDCDGQVLVMHDMLG
                     IFPGKTAKFVKNFMQGHDSVQAAVRAYVAEVKAKTFPAAEHIFAD"
     gene            15662..15838
                     /locus_tag="ACFM8S_000152"
     CDS             15662..15838
                     /locus_tag="ACFM8S_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313295.1"
                     /translation="MQTEQGLLLMPSESRFRRHFVLPCAARIIGAFCRAGSPKDKDYR
                     YANHTYHSRTARVA"
     gene            15795..16631
                     /gene="panC"
                     /locus_tag="ACFM8S_000153"
     CDS             15795..16631
                     /gene="panC"
                     /locus_tag="ACFM8S_000153"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFC6313296.1"
                     /translation="MQIIHTIRELRAWRKNAGKVAFVPTMGNLHEGHLELVREAKKRA
                     DSVVVSIFVNRLQFGQGEDFDKYPRTLQQDADKLEAEGVAVVFAPDEKELYPNVEQRY
                     NVEPPNLQNELCGKFRPGHFRGVATVVSKLFNIVAPDVACFGKKDYQQLAVIKGLTED
                     LNFDVEIVPVDTGRAEDGLALSSRNQYLSAAERDEAPRLYRELKAVAESLAQGSLDYA
                     GLEKRAVQSLTEYGWVVDYVEIRRADTLEVARAGDKKLVVLAAARLGTTRLIDNLEIK
                     LP"
     gene            16796..18634
                     /locus_tag="ACFM8S_000154"
     CDS             16796..18634
                     /locus_tag="ACFM8S_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC6313297.1"
                     /translation="MLPARFTILSVLAAALLAGQAYAAGAADAKPPKEVGKVFRKQQR
                     YSEEEIKNERARLAAVGERVNQIFTLLGGETALQKGQAGTALATYMLMLERTKSPEVA
                     ERALEMAVSLNAFEQAEMIYQKWRQIEPIPGKAQKRAGWLRNVLRERGNQHLDGLEEV
                     LAQADEGQNRRVFLLLAQAAVQQDGLAQKASKAVRRAALKYEHLPEAAVADVVFSVQG
                     REKEKAIGALQRLAKLDTEILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQEM
                     EIMNLVSLHRLDDAYARLNVLLERNPNADLYIQAAILAANRKEGASVIDGYAEKAYGR
                     GTEEQRSRAALTAAMMYADRRDYAKVRQWLKKVSAPEYLFDKGVLAAAAAVELDGGRA
                     ALRQIGRVRKLPEQQGRYFTADNLSKIQMLALSKLPDKREALRGLDKIIEKPPAGSNT
                     ELQAEALVQRSVVYDRLGKRKKMISDLERAFRLAPDNAQIMNNLGYSLLTDSKRLDEG
                     FALLQTAYQINPDDTAVNDSIGWAYYLKGDAESALPYLRYSFENDPEPEVAAHLGEVL
                     WALGERDQAVDVWTQAAHLTGDKKIWRETLKRHGIALPQPSRKPRK"
     gene            18774..19355
                     /locus_tag="ACFM8S_000155"
     CDS             18774..19355
                     /locus_tag="ACFM8S_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HFC6313298.1"
                     /translation="MKHTVSASVILLLTACAQLPQNNENLWQPSEHISSFAAEGRLAV
                     KAEGKGSYANFDWTYQPPVETININTPLGSTLGQLCQDRDGALAVDGKGNVYQAESAE
                     ELSRQLVGFKLPIQYLHIWADGRRVAGAPYRILPDGILEQYGWTVGRTADSGGQVRTL
                     QLNNGNLNIRLVFTEIGMPSETETPERCAARTR"
     gene            19365..20210
                     /gene="ispE"
                     /locus_tag="ACFM8S_000156"
     CDS             19365..20210
                     /gene="ispE"
                     /locus_tag="ACFM8S_000156"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFC6313299.1"
                     /translation="MNIADGRQAFSAPAKLNLDLRITGRREDGYHNIESIFCLIDLQD
                     TVYLKPRDDGKIILHNPVDGMPQEVDLSYRAASLLQKYARNPAGVEIWLDKKIPTGAG
                     LGGGSSDAATVLLVLNRWWQCGLTQRQLIDSGAALGADVPFFIFGKNAFARGIGDRLD
                     EMDIPKQWYVIVKPPVHVSTAKIFTHESLTRNSASSIMPTFQNLQPFRNDMQAVVFKE
                     YPEVWKAYSELSRYGFALMTGSGACVFTACQDRNSAYNIYRQVSDLYEAYLAEGLSKH
                     PLLSV"
     gene            20218..20293
                     /locus_tag="ACFM8S_000157"
     tRNA            20218..20293
                     /locus_tag="ACFM8S_000157"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20251..20253,aa:Gln,seq:ttg)
     gene            20306..20381
                     /locus_tag="ACFM8S_000158"
     tRNA            20306..20381
                     /locus_tag="ACFM8S_000158"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20339..20341,aa:Gln,seq:ttg)
     gene            20390..20465
                     /locus_tag="ACFM8S_000159"
     tRNA            20390..20465
                     /locus_tag="ACFM8S_000159"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20423..20425,aa:Gln,seq:ttg)
     gene            20632..21615
                     /locus_tag="ACFM8S_000160"
     CDS             20632..21615
                     /locus_tag="ACFM8S_000160"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HFC6313300.1"
                     /translation="MAAYDSLMVFTGNANPELAQRVVRHLDISLGNASVSKFSDGEVA
                     VELLENVRGRDVFILQPTCAPTNDNLMEILTMADALKRASAGRITTAIPYFGYARQDR
                     RPRSVRVPISAKLVANMLYSAGIDRVLTVDLHADQIQGFFDIPVDNIYATPILLNDIK
                     QQRIENLTVVSPDIGGVVRARAVAKSLNADLAIIDKRRPKANVAEVMNIIGDIQGRTC
                     LIVDDMIDTANTLCKAAVALKERGAERVLAYASHAVFSGEAVSRIASSEIDQVVVTDT
                     IPLSEAAKNCDRIRQVTIAGLLAETVRRISNEESVSYLFNEEVMTGSMLLP"
     gene            21682..22254
                     /locus_tag="ACFM8S_000161"
     CDS             21682..22254
                     /locus_tag="ACFM8S_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFC6313301.1"
                     /translation="MTYEIQASVREAQGTGASRRLRREGQIPGILYGEGQEPVAIAVD
                     HKTVFYALEKESFHTALIKLSLNGETKDVIVRDFQMHPFRREVQHIDFQAVKADQPVR
                     IRVPLHIVNAENSQAVKLQGGRVSLLNTSVEVVALPANIPAFLDLDCAEVVAGDILHL
                     SDIKLPEGVESVSLKRNENLAVATVTGKKR"
     gene            complement(22379..23548)
                     /locus_tag="ACFM8S_000162"
     CDS             complement(22379..23548)
                     /locus_tag="ACFM8S_000162"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFC6313302.1"
                     /translation="MTAHKILPVLLSIILGVSHATAASPAPNKPAVHAAPTFQTPETL
                     TAAHIVIDLQSRQTLSAKNINTPVEPAALTQLMTAYLVFKNMKSGNIQSEENLKIPES
                     AWASEGSRMFVRPGDTVSTDKLLKGMIALSANDAALTLAGRLGNGSIENFVQQMNKEA
                     RRLGMKNTVFKNPTGLSREGQVSTAKDLAQLSEALMRDFPEYYPLFSIKSFKFKNIEQ
                     NNRNILLYRDNNVNGLKAGHTESGGYNLAVSYSGNGRHILVITLGSESAETRASDNSK
                     LLNWALQAFDTPKIYPKGKTVAQIQISGGSKKTVRAGFLKEAYITLPHKEAKMAEQIL
                     ETIQPIPAPVKKGQILGKIKIRQNGYTIAEKEIVALENVEKRSRWQRLWACLTGQ"
     gene            23696..25222
                     /gene="ilvA"
                     /locus_tag="ACFM8S_000163"
     CDS             23696..25222
                     /gene="ilvA"
                     /locus_tag="ACFM8S_000163"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFC6313303.1"
                     /translation="MNTPLPYSNYLIRILTASVYDVAVETPLEPARSLSVRLKNNILL
                     KREDLQPVFSFKIRGAYNKMSKLPKDALACGVIAASAGNHAQGVALSAQRLGCRAVIV
                     MPETTPKIKVDAVKSRGGEVVLRGVSYNDAYDYAMELAEKEGLTYIAPFDDPDVIAGQ
                     GTVGMEIVSQHPDPIRAVFVPIGGGGLAAGVAAFIKQVRPEIKVIGVQTNDSCCMKQS
                     VEAGEIVHLKDVGLFSDGTAVKVVGNETFRLCKELLDEIITVDTDAVCGAVKDIFDDT
                     RSITEPAGALALAGLKAYIAREGAENQTLIAVTSGANMNFHRLRHVSERSELGEGNEG
                     IFAVTIPEERGSFLKFVNILGNRNITEFNYRYGDDEKAHIFVGLQAAGPQDLAVIGSR
                     LDEAGLPNVDLTDDEIAKIHIRYMVGGRTDKVENERLVSFEFPERPGALARFLNHMQG
                     GWNITLFHYRNHGADYGRILVGIDVPPHDAAAFDGFLESLGYSYHEETQNAAYKLFLA
                     "
     gene            complement(25279..26352)
                     /locus_tag="ACFM8S_000164"
     CDS             complement(25279..26352)
                     /locus_tag="ACFM8S_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HFC6313304.1"
                     /translation="MSITIQNLNKHFGNFHALKNINLNVPTGKLVSLLGPSGCGKTTL
                     LRIIAGLENADGGQILFDGQDVTAKHVRERKVGFVFQHYALFRHMNVFDNVAFGLTVL
                     PKSERPSKGQIRAKVEELLKLVQLSHLAKSYPHQLSGGQRQRIALARALAVEPKLLLL
                     DEPFGALDAKVRKELRTWLRDIHHNLGVTSILVTHDQEEALEVSDEIVVMNHGKIEQT
                     GSAEAIYRKPENAFVTEFLGETDAFEGRIEKGFWHYNGFAWKLDAQYKWQEQTATGYI
                     RPHEWQIAAEHETPMICAEIEKVHAVGALTHILVKHGKQDVHITLAGSDAARYPIAEG
                     KELKLIPKQVYVFSQNELIEYSI"
     gene            complement(26349..27209)
                     /gene="cysW"
                     /locus_tag="ACFM8S_000165"
     CDS             complement(26349..27209)
                     /gene="cysW"
                     /locus_tag="ACFM8S_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HFC6313305.1"
                     /translation="MKPYSANPNLTEPRWLRVLLIAAALGFLLLMLVVPLVAVFYEAL
                     KGGWDLYLKSLNDPEAWSAIKLTLITALIVVPVNAVLGVAMAWLLTRFDFRGKQLLTT
                     LLDLPFSVSPVVAGLMFVLLFGAHTALGGRLEAQGIQIIFAIPGIILATLFVTFPFVA
                     REIIPLMQAQGDSEEQAALILGASGWQMFWRVTLPNIKWALLYGIILTNARAMGEFGA
                     VSVVSGHIRGETNTVPLLVEIFYNEYNFTGAFALSGVLALLALATLAVQNIITKLQDK
                     KLAAAERNTV"
     gene            complement(27579..28415)
                     /gene="cysT"
                     /locus_tag="ACFM8S_000166"
     CDS             complement(27579..28415)
                     /gene="cysT"
                     /locus_tag="ACFM8S_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HFC6313306.1"
                     /translation="MLALKTPGVLPGFKLSLGLTVLCLSLLVVLPFAMMAAKAAEIGW
                     SGFWNTITEPNVLAAVWLSLRMSFYAMLTNVVFGTLVAWVLVRYEFPGKGLANALVDL
                     PFALPTAVTGIALATLYAPNGWIGRFFEPLGIKIAFTPVGIWIALVVVSLPFIVRAVQ
                     PVLEELSGEYEEAAATLGANRFTTFRRVLLPEITPALLTGAGMMFARATGEYGSVIFI
                     AGNIPMVSEILPLIITGKLEQFDVQGASAVALFMLLVSFVILFALNVMQWALGRRSGA
                     KG"
     gene            28554..28661
                     /locus_tag="ACFM8S_000167"
                     /pseudo
     CDS             28554..28661
                     /locus_tag="ACFM8S_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            28707..29039
                     /locus_tag="ACFM8S_000168"
     CDS             28707..29039
                     /locus_tag="ACFM8S_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313307.1"
                     /translation="MQILSFQPDIAERMLEGTEGESVNENAQFVRTDNGYWIAWHEGV
                     AALLAPDTPPGIPCFWVEGAESLEELCVMVERGEFDEVEEFDGDDDEWLETAQGCGHH
                     GDACACGH"
     gene            29371..29880
                     /locus_tag="ACFM8S_000169"
     CDS             29371..29880
                     /locus_tag="ACFM8S_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003662333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HFC6313308.1"
                     /translation="MTMILSILSLFFIIRLLFLAVSIKHEKALIAKGAKQYGKTNSTL
                     LAAVHTLYYLACFVWVWLSDTAFNGISLIGTLTVMASFVILSLIIKQLGEIWTVKIYI
                     LPNHQINRSWLFKTFRHPNYFLNIIPELIGIALLCQAWYVLLIGLPIYLLVLFKRIRQ
                     EEQAMATLF"
BASE COUNT         7114 a         6994 c         8125 g         7702 t
ORIGIN      
        1 ggtattttct taccgtattc tgcgattgcc tgtcggaaaa tgccgatcaa cctgcctata
       61 acggcatttt cgccaaattc gttcagacag ttttccctaa gtcgggcagg ttcaaaatca
      121 gagtggtgtt catacatttt gatgagtgcg tcggcaaggg catcgtcgtc gtcaacagga
      181 accaaatatc cgttgccgtc tgaaacaata gattccgcac cgccgcagcg tgtcgcaatg
      241 acgggcaatc cttgggacag tgcttcgata tagaccacgc cgaaggtttc ggtgcggctg
      301 gcaaggacga atgcgtcgct gttcctcatc aaatccaaga ctgcttcggg ctgcaatgcg
      361 cccaaaaatg taacggcatg ggtaatgccc aagtctgccg cctgctgttt cagccgctgt
      421 tcttcctgtc cgctgccgcc gatgttcagg cgcagttgcg ggcattgtgc caacgcccgg
      481 gcaaaggcag tgagtaggac atcgtgtcct ttgagatggc gaaggtgcga gacggtgcag
      541 aacacgaaat gcggtttgtt gtttttttct ctttgttcaa acggtcggtt gaatattccg
      601 cccagtatgt tgggggaggt actgccattc gcagccgtat ttgtgttgca ggacgtgtgc
      661 gaaatggcgg ctgacagcga gcagtgcgga ggcgtgcgcc gctgcttttt ccatcggctg
      721 ccattggtgg ggacggatca gaccgcgcgt gatggtgctg ctgtgttcgg ttaaaacgta
      781 gggaatgccg tatttttcag aaattttttg agcgagtatg cctgcatagt tcatgcagtg
      841 ggcgtgaatc aggtcgggca gcccgttttc gcggatgtag tgtttgaagg ctttcaaacc
      901 cgcacacacc cagcggatgc ggtcgatgtc gatgaacgga aagcggggga agaaatacat
      961 gccgtgccat gcatagatgt ccaaaccgct ttgccgatat tttgcaaaac cgtaaggccg
     1021 gtcaggatgc ttgctgtttc tttccgcaag tagcggaaca tcggtgcaag cacggcggtt
     1081 ttgatgcctt tcctctgcaa tgccagtgcc tgattttgaa aaaaatcccg tccacatcct
     1141 gttcggattg cggataccat gaggggatga cgaggacgtg caagggttcg ggcatagtgg
     1201 gattccgtat cggaaaggcg gttattataa gacagacgca gaccgaatat ttaaattgtt
     1261 gccttacgct aatgcaattt agcgcgccga tatgctagat tggcggtttt atgagtgagg
     1321 atacggatat gttgcgttct attttggcgg cttccctgct ggcggtatct tttccgacgg
     1381 cggctgaagc attgaattac aatattgtcg aattttctga atcggcgggt gtcgaggcgg
     1441 ttcaggatac aatgtccgca cgtttccaag tgacggcgga aggacgggac aaaaatgccg
     1501 tcaatgccga gtttgttaaa aaattcaaca atttcaccag aaaatcaaaa aatggtagct
     1561 ttaaaaccga attggtatcg cgcagtgcga tgccgcgcta tcaatatacc aacggcagac
     1621 gcattcaaac aggctgggag gagcgtgcgg aatttaaggt cgaaggtaga gattttgatg
     1681 agttaaaccg ttttattgcc gatattcaag cagatgccgc gttggaatat acggatttcc
     1741 atgtgtcgcg cgaacgccgc aacgaggtca tcgatcaggt cagcaaggat gccgttttgc
     1801 gtttcaaggc gcgtgccgaa aagttggcgg gcgttttggg tgcgtccggt tataaaatcg
     1861 tcaaattgaa tttgggacac atcggcagcc atatcgcggg agggggagct gctcaggcaa
     1921 aaatgcttcg tgccatgccg atggcggcaa gcgtcaatat ggagggtgcg gattctgccg
     1981 cgcagggtgt ggaggaaatc agcatcagcg tcaatgggac ggttcagttc taaccacgga
     2041 taaacaggta gatgccgtct gaaacccgac gataagggtt cagacggcat ttatatttca
     2101 ggctttgggc agggtaacgc cggtttgccc catatatttg ccgttgcggt ctttgtatga
     2161 ggtttcgcag atttcgtcgc tctcgaagaa gaggacttgc gccacgcctt cgcctgcgta
     2221 gattttggcg ggcagggggg tggtgttgga aaattcgagg gtaacgtagc cttcccattc
     2281 cggttcgaac ggggtaacgt tgacgataat gccgcagcgg gcgtaggtgg atttgcccaa
     2341 gcagacggtc aggacgttgc gcgggatgcg gaaatattcg accgtgcgcg ccagtgcgaa
     2401 ggaattgggc gggatgatgc agcagtcgtc ttcaacggta acgaagtttt tcgggtcgaa
     2461 gtttttggga tcgacgatgg tgctgttgat gttggtgaag attttaaatt cgtttgcgca
     2521 gcggatgtcg tagccgtagc tggacgtacc gtaggagatg atgcgtttgc cgtcggcttc
     2581 tttgatttgg ttcggctcga aagggtcgat catgccgaat tcttcgctca ttcggcgtat
     2641 ccatttgtcg gacttgatgc tcataatgtt ttccttgttt cttgcagtgt tcggacaaag
     2701 cattggggga tgccgtctga aaacggggct tatttgtttt tgggcagctt cacttcttta
     2761 atcatgccgt tttcgcattt cataatgaag agggtttcgc cgttttgcga gacggtaaat
     2821 ctgccgtgtg ccaagccttt tttgaatgtg cccgagagta ccatgttgcg gaatttggta
     2881 ctgtcggaat tgaacggttc gataaatatt tcgcggttgg cggcaacggt ataaacgcct
     2941 tgtccgtcga atttgccatt tttaaacgaa ccggtatagt tgcgcccgtc ttggcagcgc
     3001 catgtgcctt tgccggcggg tttaccgtct ttgccgacat cgccttcgta ggtgcagccg
     3061 ggttcttgat aggaagtcag aacggcggac gcggcgggga gggcgaacat catggcgggc
     3121 agtaggaatg cgagatgttt aagcataagg gttattccat tggattttgg ctgacggtat
     3181 aaaagccgtc tgaaaaatca atcttgccag ccgcccaaat aggaaaccag ctcttccaac
     3241 atgatgctga cggattccgc catcaggatt tgcgaggcga aggcgagacc ggcggcatcg
     3301 tcgccgttgc tttcggcttc ttcctgcaat acgtcgaggt attggatgcg cttgagtgtg
     3361 aagtcttgcg tgaggataaa ggcaatttgt tcgcgccaca ccaagcccag ttgggtaacg
     3421 gttttaccgt ttttgacgtg ttgaaccact tcgtcggcgg ttaaatcttg tttggatact
     3481 ttgacgacgg gaacaatatc gcccgtgcct ttgagttcgc aatcgctgtc caattcaaaa
     3541 ccgccttcgc aatgcccttg caacagccag ccggtcatca gggaagaggg cgattgtttg
     3601 gtattcggca gtgaggcttc caaaccgccc aaagcttcgc gcagcttggt caggatgttt
     3661 tctgctttgg cggaagccgc gttattgacg agcaggtagc cgtggcgggt gttaaacacc
     3721 gcttctgtac ggctgctgcg ggtaaacgct cggggcagca ggtcgtctgt aatttgctct
     3781 ttaagctctt gtttttcttt gcgaccgaca ttgcgggctt cattgttttg gatttccgct
     3841 accttctctt ccaaaatatc gcggatgacg ccggcaggca ggactttttc ctcttttttc
     3901 agggcgacgc gcaaggtaaa gtcggcagga aaaacgagtt ccggggagaa tgaaaccggt
     3961 gcggtaaagc cttcgctgaa ccagtctaag ccttggcaat gggtaaattc agcttcagca
     4021 agtttgtcgg caagtacgtc cgcctcaggc agcttttctt tgttgagcgg ataaaaacta
     4081 atctgcttga accacataat gtttcctgtt gtttgaaatg tcgggaatta tttgctgaat
     4141 tgttttttca cactgacttt ggttttcttc ttgaagcggt ttttctcttc tttgcggcct
     4201 tcgcgtttct caatacggtt gctcaggctg acgcggcgca gcggtttttt cttcggtttg
     4261 tcgtcggcgt tttcatacgg gttttccgaa acattgtatt gaattctgag cggcgtgcct
     4321 tgcagattga aggctttgcg gaacgtttgg gtcagatagc gcgtatagct gtcggaaatc
     4381 gcgtgcagcg aattgccgtg taccacaatt acgggagggt tcatgccgcc ttggtgggca
     4441 taacgcattt tcggacgcac caagccggca cgcggcggtt gctgacgctc gattgcgctt
     4501 tgcaggacgc gcgtgatttt cggcgtcggc atcttaatca tcgccgcgtt gtaggcagcc
     4561 tgaatgctgt caaacaaacc gtctataccg cgctctttca atgcggaaat aaagtggaac
     4621 ttggcaaaat cgaggaaata cagtttgcgg ttgatatcgc gtttcacttg ctcgcgacgt
     4681 tcttcgctga tgccgtccca tttattgacg gcaaccacca aggcgcggcc tgcttccaag
     4741 gcaaaaccgg caatcgtcgc atcttggtcg gcaatgtcct gttgcgcgtc caacaccaaa
     4801 acagcaacgt ttgccgcctc aaccgcctgc atggctttga taacggagaa tttttccacc
     4861 gcttcatcca ctttgccgcg acggcgcaca cctgcggtat cgatgatggt aaacggtttg
     4921 ccttcgcgct caaaatcaat gtggatgctg tcgcgcgtcg tacctgccat atcgaaggca
     4981 atcacgcgct cttcgccgag aatggcgttt accagcgtgg atttgccgac gttcggacga
     5041 ccgataacgg caaaaacagg atgtttcgcc tcttcctctt ctttttcagg ctcagggaag
     5101 gtttccaaaa tatcttcaat cagataatac acgccgtcac cgtgcgcacc tgaaataaca
     5161 taagggtcgc ccaaagcaag ttcgtagaac tcggcggcaa gcacggccct attgccgcct
     5221 tctcctttat tcacggccaa ataaacgggg cgcgggcttt ggcgcaaacg gtcggcaatg
     5281 attttatctt gcggcgtcag accggtacgg ccgtccacca aaaacacaac cgcatcggct
     5341 tcatcgacag cctgtaaggt ttgttttgcc atttcgtgca aaatgccgct gtccacaacc
     5401 ggctcgaaac cgccggtatc gatgaccaaa taaggtttgc tgccgacttt gccgtgtccg
     5461 taatggcggt cgcgggtcag gccgggcagg tcgtgtacga gtgcgtcttt ggtgcgcgtc
     5521 aaacggttga ataaagtaga tttgccgacg ttggggcggc cgacaagcgc gatggttggt
     5581 ttcatgattg agtctttctg tgtcaagtgc cgttcgggag aactgaacac gagcaggtgt
     5641 ccgttgggac acggcggatg ggttttacgg gaattgccgt agaatggggt tgtctgaaat
     5701 gccgtctgaa gagagggcgg tatttcagac ggcatttatt tcagcgaatc aagtttcatt
     5761 tgaaccaatt cgcgaccgac agaatcttga ggcatttttt ctaaagcctg tccgtagttt
     5821 tttaaggctt cctggctttt tccctgtgcg gcatagacat cgcctttggt ttccatcagc
     5881 aggggggcga agtccgcttc aaccggcgtg tcgagcgcgg caagtgcggc atcgtatttt
     5941 ttttgttgca acaacacaac gcccagacgc tgcgccgcca acgcttgaat caggctgtct
     6001 ttttggttgg acaacaccca tttcaaatgg ccttcggcaa catcgtaacg ctgtgcgtca
     6061 aattcggttg ccgccgccat cagcgtggct tgggcggcgg aaatggaatg cgggtagctt
     6121 tgttggagtt tggtcaattc ggcattgatt tcgctttgcg gggctttgtt ttgcgccttt
     6181 tccacgatgt ttgccagcac cgccgccgct tcctgatttt gggaagccgc acggttttgg
     6241 taaaccgtgt atcccaagta gccgagcgcc gccaaaatca gcacggcaaa cagccatttg
     6301 cccgtggttt tccaaaaata tttaaagttg tctaactctt gttgttcttc gagatgggct
     6361 gccatttatg cgttcttcca ttgttgtaaa gtatgggtta aatcctcggc ggcgacagtt
     6421 tgctgaccgt gtgcgccgtt catgtctttg agcgtaaccg taccgttcgc cagttcgtct
     6481 tgcgcgacaa tcagggcaaa gcgtgcgccg ctgttgtcgg cttttttcat ttgtgctttc
     6541 aggctttgat agccggaata ctgcattaca ttgaaatctt gagcgcgcaa ggcttgtgcg
     6601 tatttcatca cctgcaagtc cgcccctttg ccttggtgca ttgcatagac atcaggcgcg
     6661 gcgttcactt ccagcgaacc gtattcgctc accaaaagca gcagccgttc gatgcccatc
     6721 gcaaagccga tggacggcgc aggcttaccg ccaagctctt caatcaaacc gtcgtaacgg
     6781 ccgccgccgc acacagttgc ctgcgcgccg agtttgtcgg tcgtccactc aaaaaccgtt
     6841 tgattgtaat aatccaaacc gcgaaccaag cgcggatttt caatatattg gatacccaaa
     6901 ccgtccaaca tcgctttgaa gcgtgcatag tggttttgcg aagcctcgcc caaataatcc
     6961 accaaacgcg gcgcggcgtt gcagatttcc tgcaaatcag ggtttttcgt atccaaaacg
     7021 cgcaaaggat tggttttcag acggcgtttg ctgtcttcgt ccaatttatc ttcatagcgt
     7081 gtcaaatatt caaccaacgc cgcacggtgt gccgctcgct cctcacggtt gcctaagctg
     7141 ttgatttcca aagtcaggta ttcgcggata cccaattttt cccacaagtc ggcagacatc
     7201 gcgatgattt ccgcatcgat atccggccct tcaaaaccca aagcctcgat accgacctga
     7261 tggaattgac gataacggcc tttttgcgga cgctcgcggc ggaacatcgg ccccatatac
     7321 cacagctttt gcgggctgtt gtacagaagg ttgtgttcga ccaccgcacg cagacaagaa
     7381 gccgtacctt ccggacgcag gctcaaactc aaagaatcgt ttgaatcgga aaaggtgtac
     7441 atttccttgc cgaccacatc ggtttcctcg ccgatggagc ggacaaacaa accggtttgc
     7501 tcgacaatcg gcgtacggat ttgctgatag ccgtaagcgc gcgtccagcg gtttaccgta
     7561 tcctcaaacg cctgccaaaa cgcagccgtc agtttgaaat ctttttgctc gacaggcaga
     7621 aggtcgttca tgcctttgac ggattggatt ttttgtgcca tttcaagtaa gaatgcttaa
     7681 atcaaattgc gggcgattat agcggatttt taagggtttg tgaggttggg ggtggtttgc
     7741 ggacgggtaa aatgccgtct gaaacagttt tcagacggca tttgacttac tctgcacgtg
     7801 cttgcctgat ttgtcggact gcaaactctg tctgccgctt gggatgatgt gtgaaaaaca
     7861 atttatttga aattgtctcg gcttttttcg gcacgaccgc caaaatctta cctgccaaat
     7921 ttccctcacg ggtttgccaa gcatccaaaa actgcgccct gctcatcgaa acatgaccca
     7981 gcgacgggtc ggcaagcaaa accgtattgc cgtctatacc gcgcaatacc gagaaatgat
     8041 catccttacg gtatttcaga tacacgatga cggggatttt caactgcgcg agctgctcga
     8101 aagacagggc atagcctttc gcttcaaaac ccaaatctgg cataatgcgc cgcatatcct
     8161 caaacgacgc gcgcatctgc tccttatcca gctttttcaa cacttcttct tccgtcagcg
     8221 tttgcccgta aaaattgttc aaaagcgtcg ccaccgaagc cgccccgcag gaaaaatcca
     8281 aatcctgctt tacaatattg aaatcgcgcc tctctttcca actctgtatt ttgactttgc
     8341 cgtaggctat ggggttttct tccgcaaagg gaaaagaatg cgcacaaagc ggcaggacgg
     8401 cggcgaggag cagggaagcg gcaaaccgcc ttttttgttc catcaaaatc ctcctgaaag
     8461 gctgcctgaa atttccaagc agcctttttg attgcttatt aatcggatag tgcgacaaat
     8521 ataaagtggg cggcaatcat tgctgtaacg gcagccataa ctgttttatt ttcttcaaac
     8581 caaagcaagt tgagaaacat aaacatcaag aaatatgatg gttcttcagt tttgccgctg
     8641 ctttcttgag ttagaagata aaatatagat atggcggtta tgctgattgc tgcttttgtt
     8701 aaaatatatt tgtttttcat catcttgtat ggttttttat gcaagtttca acaataaaat
     8761 tcggaataag atagagagat gttgatatat cagataaata tacaaaacga tcaagaaact
     8821 attggcagta tgtataatct tgaatttctt tttttgatga aattaaaata atgaaatata
     8881 tggtaaacgt tacaacggaa gccttagcct cccgattgta gtatttcaat tccaagataa
     8941 atgccgcata gaaaagataa aatacttaaa tggtttttag cggcttaaat atcttattta
     9001 attttattga ttgttttccc aatagtttcc gcaataaata ataagaagta gaataataaa
     9061 caaacagaac atgaaaataa aaataaatct ttcatcatct cagtaataaa attccaaaga
     9121 ttttccttat ttccatcaac aataaaaatt atcagttcat gaataaagaa tactgcaaag
     9181 aaaatgcttc ctgccgttag agatatttct aatctgctta ttttcccttc gctatattta
     9241 cctgataaat agcctaaaaa agctgcaatc aaatcatcca ttttaaaaat ttctgtaaaa
     9301 acaggtaatg tctttaattc attacctgtt ttgccactat atctatgata taagcgtctc
     9361 ttacccttca gcctctttgg gaaatcaaag ccctgcttag aacgtatgtt gcacacccaa
     9421 tttcaatagg taaatcagat tgcaaatcca gcaatttgaa tattgtcatt gttccgtgca
     9481 aaagggattt ttattgatga gttgtgtact gggtttcagc ttggcttttt agataatctt
     9541 ttcacaagaa catacatcaa tactgtccaa aaaaaaagat ttgataaata tattgcaaca
     9601 tattttattc caccttcatc cgaaacagaa accaatatat ttttaattaa tacatagctg
     9661 attattatat ttattattcc tataatatat aaggatcttg ccaaaatttt ttttgatttt
     9721 attttaggaa tatctcttat ccatgctaaa atacagccca atgtcgaaaa gaaaataaca
     9781 gcaaacacta ccttacctcc tctcacttcc aaccaaaccg atagcaggtt tggttggaag
     9841 ttggtttatt tattatttaa cggcgaatgt cagtgttctt ccccgtagaa cctgcataac
     9901 catttatgcc accagcaaca gtccccactg ctacacctcg cataccatta atcagaattc
     9961 ttcctatttt ttcgaatcca ctatttcttc caaagatgca gcaaacgcag gttgagccta
    10021 gacccaatct acgtttgccg ccgcatctga tccctattgt ttcttatcct tacatcttcc
    10081 tgccttgtca atcaaataaa gacagaagac tatacaaaaa ctgaccgaca tactgaaaat
    10141 acctatcccc ttccatgcaa tctccgcacc attgacccaa tagatcagac tgagaaacaa
    10201 caaagccaca gtataaatca acgtaaaaaa tattgcgtaa agcacaataa agggaacttt
    10261 tatctcacgg ttgtttttta taatatattc agcaacttga tttccgaata tacctgataa
    10321 aaaatatagt attagatcat ccatttcgtt tatcttctat gttttcccat tgcggcgcta
    10381 gaagactgat ttaatgctgc accatttgca cgaataactg cattaggaat agcatttccc
    10441 cctttccaag cagaacgggc aaaaacacta gtagtaatcc ctgcaccgcg caacatcgca
    10501 ctgctatatc cgcctcctat cataccacca gcggttgcag ccaaggtact tcttgttgaa
    10561 gcaaattgcc ctgttttaat tttagaaatg ccgtgattag cccaagcact aattgcccct
    10621 cccatcaaag cacctgccac aataggaaga aacgccccct cagtctcctt catctccttt
    10681 tgagaaagct ccgccaactg catcggtgca tctgcccgcg tgtggaacac ttggttttca
    10741 aatgtctgat tgtccaagcc gtttgccatt gcgggggcaa tcatagacag catcattacg
    10801 gctgcggtga tttgtttttt cataataact cctttggatt acaaggttgg aaaatcaaaa
    10861 cctgcttaaa atgtatgctg tacgccaaat ttcagttcgg aactgctttg ccctgaaacg
    10921 ttgaaacgtg cggatgcgtt taaagccgtg gttttggtga aaccgaaacc tgcgccgaaa
    10981 tgggcatagg tggatgtgtt tctggaggat tcccgtttgc cgtccgtccg gtcgggctgc
    11041 ctgcccaacc attggatgcc tccggtcagg ctgattctgt cgttggcggc aaatgagatg
    11101 ttggggttga gcagcaggta gttgcccgat ttgtagcgga tgccgtctga aagggttttg
    11161 ctgccgttga tacggtaggc ggcggtgagg gaaaggacaa tcggatctat ggctttgtag
    11221 gtggtggcgc cgatgagcca ggattttccc gacgaggctt tgttgcgcga tttttcgtaa
    11281 accgtgcttt caagaaagct gattagggcg gggtttttgt cgtctttaag gaaagtgtgg
    11341 ctgatgccga gggatacgtc ggacatccgt ttgttgcggg ttttgctgtt gccgtcgagt
    11401 ttgcgttctt cgtgccacag atagctgccg ctgccgtaaa tgtcggtatt cccggtcagt
    11461 ccgtagcgca aaccgagcgt gccaacgagc atatcggtat tgctgccgtt ttcttggatt
    11521 tcggtcggaa tggggataaa cgaggttgcg ccggtttgga tgtaaaccgg tgcggcaagt
    11581 gcggcgcggc tgttttcgct attcaggtag gtaagggaag tttccagttt ccatttgccc
    11641 ttgtcggtta ttatgtcttc aatcgtcaag ggcaggtcgg cataagcgga taaaggcagg
    11701 atggcgggca aggcggacaa aaagatacgt ttcatatttc ctattattga tattaattct
    11761 tatttaagaa tagtaatgaa tatccgataa ttctgtctaa ataaattttt aaatcaaaaa
    11821 aaacgatata tttgttttta tcaattatgt tttgatatgc cgtctgaaaa gtttgtgaaa
    11881 aacggttaca atccgccgca tgaaaaaacg caataatcct cttccgttgt tgaacggtgt
    11941 caaacccagt tatttggtgc tgccgcatga aaagcagttt tacgggctgc cgctgctgca
    12001 ttttctgtgc atccgctttc cttttgtggg cgcggatgat tggcgcaggc ggttgaacag
    12061 cggttttgtg gtcggttcgg atggtgcggc gttggacgaa cattctttgt tcgagccggg
    12121 taaggtgatg ttttattacc gtgaaaccag ccgtgagagc gagccgcgta ttccgtttga
    12181 agaaaagatt ttgcatattg atgagcattt gattgtggtg gacaaaccgc attttctgcc
    12241 cgtcatcccc agcggcaggt ttttgcggga aaccctgctc acgcgcctgc gtttgcggcc
    12301 tgaattgcag catttgaatg ttgaggacat tacgccgctg caccgcttgg acaaggatac
    12361 ggcaggcgtg atgctgctgt cgcataatcc tgccacgcgc ggagcctatc agacgatgtt
    12421 tcaaaacaaa acggtatgga aaacgtatga ggcgcttgcg ccgacaagga cggatttgcc
    12481 gtatccgctc gatgtggttt cgcgtttggt gaggggtgag aaatttttta cgacgcaaga
    12541 ggcggaaggc gaaccaaacg cacatacgac ggtcgaactg attgaaaaca ggggggaatt
    12601 cagcctttac cgccttacgc cgcataccgg caagaaacac cagttgcgcg tgcatatgat
    12661 gggtttgggt atgccgctgt tgaatgacgc gctctatccc gtgccgtctg aagcgggcag
    12721 cgaggattat cggaaacctt tgaagttatt ggcaaaaaag attgcgtttg cagatccttt
    12781 gtcgggtcgg gaaagggtgt tttgcagtag gttttgccta taatgagagg aaatgcaaac
    12841 ccgtatcggc agatgccgcc gatacgggtt ttacattatc gatgccgtct gaaaggggtt
    12901 ttatccgttc aggcatacgg gcgcgaagcg gaggatggtg tcgcgcagaa cgatgagttt
    12961 gccgtgaaag aaatgcgtgg aaccggcgat ggtaatgacg ggcaaatctt gcggttccgc
    13021 ccatttcagg gctttttcga tttcgacgac ttcgtcttcc gcgccgtgta tcatcagcgt
    13081 tttggcaaca ttgggaacgg cggacggctc ggggcggccg gtatagtggc agactgccgc
    13141 gccgatgagc agcaataaat cgggtatgcg cgcttgtgcg gcaaatgtgg cgacataacc
    13201 gccgaaggaa aagccggata aggcaaattc gggggcttcg ggatgttggg cgcgggcata
    13261 atcgatgacg gcgaggcagt cttgcgtttc gccgcgtccg taatcgtgtg tgccttcgct
    13321 gttgcctacg ccgcgaaggt tgggcaggta gcagtggaag ccaagtttgc ttaaggcttt
    13381 ggcggcggtt tggatgactt tgttggtgtt tgttccgccc tggagggggt tgggatgatt
    13441 gatgacggca acaccgcgtg caggtacttg ttcggacggg atatggatgg tttctaaaat
    13501 gccgacagga ccgggtatgt gtatggtttc gggtttcagc ataatggttt cctcttgatt
    13561 ttccgcatat tccgatgcaa ccgtaaagat agttgcttaa aggtggaaat gcttgccgtt
    13621 gttggggttg gatgctttca ggccggcgag catgcggtgg aagtccaatc cgtatgcgct
    13681 gctcagtttg tcggcgcgtt tcttgagttt gtctatgttt tgctctttgg ggctgctttg
    13741 gaatgccatt accgccacat tgccgtggct ttcggcagga agttccaaga cgcgcccttc
    13801 aaaaacgctc aacaaccgtt cgatgaagcg ttgatagcgt ttgtcgccgc tccaccagtt
    13861 ggttacgaat atgccgtctg aagagagcgc gttgcggcag tctcggaaga acggttcttc
    13921 aaccagcgta tcgatgattt gttcgccgtc gaacccgtcc accaaaatca catcggtgtt
    13981 gtggcggaag actttgatat attctgcacc gtctgcttcg ataatttcaa atttctcgcc
    14041 ctcgaaaggc aactcgaaca ggctgcgggc aatggcgatg acctgaggat tgatgtccac
    14101 ggcggtttgg cgcgtgtcgg gcaggtaggt gtctatccag cgtgcaaacg aaccgccgcc
    14161 caagccgatt tgggtgatat gttgcggcag gcgttccgca aacagcagcc agcccatcat
    14221 cgcgcggctg taagagagca ccagctcgga cgggcggtcg aggttcatcg agctttggac
    14281 ggtgttgctg cccaagtgaa gcgaacggat attgccttct tcggaaatgc cgacttcggg
    14341 aaagcaggat tttgcaggac gcaggcggcg gtagggatgt cttgccatcg gtgtgaacgg
    14401 tcggtttgaa aggcagatat tttatcagaa tgccgatggt tgtcctgctt caaattaatt
    14461 tcttttcaaa taaattactt attcggattt gccggggctt tcggataaat tccttgccaa
    14521 ggtgcggcat tgcctgcata attcgctttc tttgccggta tagctcagtt ggtagagcac
    14581 ctgacttgta atcagggggt cccgagttcg actcttggtg ccggcaccat ccttgcttat
    14641 ggtgcggtaa tacaatccaa tatgccggta tagctcagtt ggtagagcac ctgacttgta
    14701 atcagggggt cccgagttcg actcttggtg ccggcaccag atttgacagt ccgattgtgt
    14761 aaaacagtcg ggctgttttg catttgcggc acggtcggtg gcagggctgc gggcatttca
    14821 ctataatagc ccgtttcaaa ctgaaaaggc cgtctgaaaa gggcggggta acaatatctg
    14881 atgattactg tgaacacact gcaaaaaatg aaggcggcgg gcgaaaaaat cgctatgctg
    14941 accgcttacg aatccagttt tgccgcgctg atggacgatg ccggcgtgga aatgctgctg
    15001 gtcggcgatt ctttggggat ggcggttcag gggcggaaat cgacgctgcc cgtcagcctg
    15061 cgcgatatgt gctatcacac cgaatgtgta gcacgcagtg caaaaaatgc gatgattgtc
    15121 agcgatttgc cgtttggtgc atatcagcag agtaaggagc aggcgtttgc cgccgccgcc
    15181 gaactgatgg cggccggcgc gcatatggtc aaactcgaag gcggtgtgtg gatggcggaa
    15241 acgactgaat ttctgcaaat gcgcggcatt ccggtttgcg cgcacatcgg tctgaccccg
    15301 caatcagtgt ttgcctttgg cggatacaag gttcagggtc gcggaagtaa ggcgcaggcg
    15361 ttgcttaacg atgccaaggc gcacgatgat gcgggggcgg cggttgtatt gatggagtgt
    15421 gttcctgcgg aactggcaaa aaaggtaact gaaactgttt cctgtccgac tatcggcatc
    15481 ggcgcggggg tcgattgcga cgggcaggtt ttggtgatgc acgatatgct cggtattttt
    15541 ccgggcaaga cggcgaaatt cgtcaaaaac tttatgcagg ggcatgatag tgttcaagcg
    15601 gcggttcggg cgtatgttgc cgaagtcaaa gccaaaacct tccctgctgc ggaacatatt
    15661 tttgcagact gaacaaggtt tgctgctgat gccgtctgaa agccgtttca gacggcattt
    15721 tgttttgccg tgcgcggcgc gtataatcgg cgcgttttgt cgggcaggaa gcccgaagga
    15781 taaggattac cgttatgcaa atcatacata ccattcgaga actgcgcgcg tggcgtaaaa
    15841 atgcgggaaa ggtggcattt gtgccgacca tgggcaatct gcatgaagga catcttgagc
    15901 ttgtgcgtga ggcgaaaaaa cgcgcggaca gtgtcgtggt cagcattttc gtcaaccgcc
    15961 tgcaattcgg tcagggcgag gatttcgaca aatatccgcg tactttgcaa caggatgcgg
    16021 acaagctgga agccgagggt gttgccgttg ttttcgcgcc cgatgaaaaa gaactctatc
    16081 cgaacgtgga gcaacggtat aacgtcgaac cgcccaacct gcaaaatgag ttgtgcggca
    16141 agtttcgtcc gggacatttc cgcggggtgg cgacggtggt gtccaagctg ttcaatattg
    16201 tcgcgccgga tgttgcctgt ttcggcaaaa aagactatca gcagcttgcc gtgattaaag
    16261 gtttgaccga ggatttgaat tttgatgttg aaatagtgcc tgtcgatacg gggcgcgcgg
    16321 aagacggttt ggcactctcc agccgcaacc agtatttgag tgcggcggaa cgcgacgaag
    16381 caccgcgcct gtaccgcgaa ttaaaggctg ttgccgaatc cttggcgcag ggcagtttgg
    16441 attatgcagg tttggaaaaa cgtgccgtcc aatccctgac agaatacggc tgggtggtcg
    16501 attatgtcga aatccgccgc gccgatacgc tcgaagtggc gcgggcggga gataagaaac
    16561 tggtggtctt ggccgccgcc cgtctgggga cgacgcgcct gattgacaat ttggaaataa
    16621 aactccctta aaccgcaagc gtcgggaatg ccgtctgaag cggatttgcg tttcagacgg
    16681 catttatttt tttgaacggg gtttcgcaaa tctacaaacg attctgcttg tgataaagtt
    16741 atgcctgatt atatgccgtc tgaaggttcg gacggctgtc ggataaagga agattatgtt
    16801 acccgcccgt ttcaccattt tatctgtgct cgcggcagcc ctgcttgccg ggcaggcgta
    16861 tgccgccggc gcggcggatg cgaagccgcc gaaggaagtc ggaaaggttt tcagaaagca
    16921 gcagcgttac agcgaggaag aaatcaaaaa cgaacgcgca cggcttgcgg cagtgggcga
    16981 gcgggttaat cagatattta cgttgctggg aggggaaacc gccttgcaaa aggggcaggc
    17041 gggaacggct ttggcaacct atatgctgat gttggaacgc acaaaatccc ccgaagtcgc
    17101 cgaacgcgcc ttggaaatgg ccgtgtcgct gaacgcgttt gaacaggcgg aaatgattta
    17161 tcagaaatgg cggcagattg agcctatacc gggtaaggcg caaaaacggg cggggtggct
    17221 gcggaacgtg ctgagggaaa gaggaaatca gcatctggac ggactggaag aagtgctggc
    17281 tcaggcggac gaaggacaga accgcagggt gtttttattg ttggcacaag ccgccgtgca
    17341 acaggacggg ttggcgcaaa aagcatcgaa agcggttcgc cgcgcggcgt tgaaatatga
    17401 acatctgccc gaagcggcgg ttgccgatgt ggtgttcagc gtacagggac gggaaaagga
    17461 aaaggcaatc ggagctttgc agcgtttggc gaagctcgat acggaaatat tgccccccac
    17521 tttaatgacg ttgcgtctga ctgcacgcaa atatcccgaa atactcgacg gctttttcga
    17581 gcagacagac acccaaaacc tttcggccgt ctggcaggaa atggaaatta tgaatctggt
    17641 ttccctgcac aggctggatg atgcctatgc gcgtttgaac gtgctgttgg aacgcaatcc
    17701 gaatgcagac ctgtatattc aggcagcgat attggcggca aaccgaaaag aaggtgcttc
    17761 cgttatcgac ggctacgccg aaaaggcata cggcaggggg acggaggaac agcggagcag
    17821 ggcggcgcta acggcggcga tgatgtatgc cgaccgcagg gattacgcca aagtcaggca
    17881 gtggctgaaa aaagtatccg cgccggaata cctgttcgac aaaggtgtgc tggcggctgc
    17941 ggcggctgtc gagttggacg gcggcagggc ggctttgcgg cagatcggca gggtgcggaa
    18001 acttcccgaa cagcaggggc ggtattttac ggcagacaat ttgtccaaaa tacagatgct
    18061 cgccctgtcg aagctgcccg ataaacggga ggctttgagg gggttggaca agattatcga
    18121 aaaaccgcct gccggcagta atacagagtt acaggcagag gcattggtac agcggtcagt
    18181 tgtttacgat cggcttggca agcggaaaaa aatgatttca gatcttgaaa gggcgttcag
    18241 gcttgcaccc gataacgctc agattatgaa taatctgggc tacagcctgc tgaccgattc
    18301 caaacgtttg gacgaaggtt tcgccctgct tcagacggca taccaaatca acccggacga
    18361 taccgctgtc aacgacagca taggctgggc gtattacctg aaaggcgacg cggaaagcgc
    18421 gctgccgtat ctgcggtatt cgtttgaaaa cgaccccgag cccgaagttg ccgcccattt
    18481 gggcgaagtg ttgtgggcat tgggcgaacg cgatcaggcg gttgacgtat ggacgcaggc
    18541 ggcacacctt acgggagaca agaaaatatg gcgggaaacg ctcaaacgtc acggcatcgc
    18601 attgccccaa ccttcccgaa aacctcggaa ataatgcagg tccatccttt cagacggcat
    18661 aaggtttgcc gggaagccgg ggcattcggg caaacggcac gcagttcgca cgcgttttgc
    18721 acggcacgcc gaacccatcg gccggcagga tggcatccgt taaggaaatt ctgatgaaac
    18781 acaccgtatc cgcatcggtc atcctgcttt tgaccgcttg cgcgcaatta cctcaaaata
    18841 acgaaaacct gtggcagccg tccgaacaca tcagcagttt tgcagcagaa gggcggttgg
    18901 cagtgaaagc ggaagggaaa ggttcgtatg caaatttcga ttggacatac caaccgcccg
    18961 tggaaaccat caatatcaat acccctttgg gcagtacgct cgggcagttg tgtcaagaca
    19021 gggacggcgc attggcagtg gacggcaaag gaaatgtcta tcaggcggaa agtgcggaag
    19081 aattgagcag gcagctggtc ggtttcaaac tgccaatcca atatctgcat atctgggcag
    19141 atggcaggcg tgtggcgggc gcgccttacc gcatcctgcc ggacggcata ttggaacaat
    19201 acggttggac tgtcggcaga accgccgaca gtggggggca agtccgaacg ttgcaactga
    19261 ataacggaaa tttgaacatc aggctggttt tcaccgaaat cggtatgccg tctgaaaccg
    19321 aaaccccgga acgctgtgcg gcgcgcacga gataaggcgg acagatgaat attgcggacg
    19381 gacggcaggc gttttccgca cctgcaaaac tgaatctcga tttgaggatt accggcaggc
    19441 gggaagacgg ttatcacaat atcgaaagca tattctgcct gatagatttg caggataccg
    19501 tatatttgaa accgagggac gacggcaaaa tcatcctgca caatccggtt gatggaatgc
    19561 cgcaggaagt agatttgagc taccgtgccg catcgttgct gcaaaaatat gcgcgcaacc
    19621 ccgccggcgt ggaaatatgg ctggacaaaa aaatcccgac aggggcgggt ttgggcggcg
    19681 gaagctcgga tgcggcaacc gttttgctgg tgttgaaccg ttggtggcag tgcggtctga
    19741 cgcagcggca gctcattgat tcgggcgcgg ctctgggggc ggacgtaccg ttttttattt
    19801 tcggcaaaaa tgcgtttgcg cggggtatag gcgacaggct ggacgaaatg gatattccga
    19861 aacagtggta tgtcatcgtc aaaccgcccg tccacgtttc cactgcaaaa attttcacac
    19921 acgaaagctt gacacgaaat tccgcctcaa gcataatgcc gactttccaa aatctgcaac
    19981 cgtttagaaa tgatatgcag gcagttgtat ttaaagaata ccctgaagtt tggaaagcct
    20041 attccgagtt gtcccgatat ggatttgcct taatgacagg ttccggtgcg tgtgtattca
    20101 cggcgtgtca agataggaat agcgcataca atatataccg acaagtttca gatttgtacg
    20161 aggcatattt ggcagagggt ctttcaaaac atcctttgtt gtccgtataa acattgttgg
    20221 ggagtcgtca agcggttaag acactggatt ttgattccag catgcgaagg ttcgaatcct
    20281 tcctccccag ccaagtcaaa cgagttgggg agtcgtcaag cggttaagac actggatttt
    20341 gattccagca tgcgaaggtt cgaatccttc ctccccagcc agcccttttt ggggagtcgt
    20401 caagcggtta agacactgga ttttgattcc agcatgcgaa ggttcgaatc cttcctcccc
    20461 agccaaataa aagcgtgtaa gcctgcttac acgcttttat ttcatagaaa taaaaatatt
    20521 gaaatgcctt tgtttgtgtc ggatgttgca ggtataatgt cgggcttggt acaagcagag
    20581 ggaagcattg tgttttctga gcggaagtta aacataaaat caggtgagaa tatggctgcg
    20641 tacgacagtt tgatggtatt tacaggcaat gccaatcccg aattggcaca acgtgttgtc
    20701 aggcatttgg acatttcttt gggcaatgct tccgtatcca agttttcaga cggcgaagtt
    20761 gccgtcgaac tgttggaaaa cgtacgcggg cgcgatgttt tcatccttca gccgacctgt
    20821 gcgccgacca atgacaacct gatggaaatc ctgacgatgg cggatgcact gaagcgtgct
    20881 tcggcaggtc gtattaccac agccattccg tatttcggct atgcgcgcca agaccgccgt
    20941 ccgcgttccg tccgcgttcc gatttctgcc aaactggtgg caaatatgct gtattcggca
    21001 gggatcgacc gtgttttgac tgtcgatttg catgccgacc agattcaagg tttcttcgat
    21061 attccggtgg acaatattta tgccaccccg attctgttga acgacatcaa acaacagcgg
    21121 attgaaaatc tgaccgtcgt cagcccggac atcggcggtg tcgtccgcgc ccgcgccgtg
    21181 gcaaaatccc tgaatgccga cttggcaatc atcgacaaac gccgcccgaa agccaatgtg
    21241 gcggaagtca tgaacatcat cggcgatatt caaggtagaa cctgtctgat tgtggacgat
    21301 atgattgaca ctgcaaatac gctgtgcaaa gccgccgtcg ccctgaaaga gcggggggct
    21361 gaacgtgttc tagcctatgc cagccacgcc gtattctccg gagaggcggt cagccgtatc
    21421 gcctcatccg aaatcgacca ggtggtcgta accgatacca ttcctttgtc tgaagcggct
    21481 aaaaactgcg accgtatccg tcaggtaacg attgccggtc tgttggccga aaccgtccgc
    21541 cgcatcagca atgaagaatc cgtctcatat cttttcaatg aagaagtgat gacaggcagc
    21601 atgttgctgc cataagcccg aagccgtctt aagctggtcg cggccgatga cggcgatttt
    21661 acctaacttg gagtatttaa catgacttat gaaattcaag cctctgttcg tgaagcacaa
    21721 ggcactggtg cgagccgccg cctgcgtcgc gaaggccaaa tccccggcat tctgtacggt
    21781 gaaggtcaag agcctgttgc aatcgctgtg gatcacaaaa ccgtattcta cgcattggaa
    21841 aaagaatctt tccatactgc gttgattaag ttgtctctga acggtgaaac caaagacgtt
    21901 atcgtccgcg atttccaaat gcacccgttc cgccgcgaag ttcaacacat cgacttccaa
    21961 gctgtgaaag cagaccaacc cgtacgcatc cgcgttcccc tgcacatcgt taacgctgaa
    22021 aattcccaag cggtcaaact gcaaggcggc cgcgtatctc tgttaaacac ttctgttgaa
    22081 gtagttgctt tgcctgccaa catccctgct ttcttggatt tggattgtgc cgaagtggtt
    22141 gccggcgaca ttctgcactt gtcagacatc aaactgcctg aaggtgtaga aagcgtttcc
    22201 ctgaaacgta acgaaaatct ggctgttgct accgttaccg gtaagaaacg ctaattgatt
    22261 tcagcagcag ggcggtgcgt atgcaatacg taccgccctg ttgttttatg ccgtctgaac
    22321 cgtgtttcag acggcatttc tttatttgtt ggaaaaacgg gatatttgaa acggcagatt
    22381 actgccctgt cagacacgcc caaagccttt gccaccggct tcttttttct acattttcca
    22441 gtgcgacgat ttctttttcg gcaatggtgt atccgttttg cctgattttg atttttccta
    22501 aaatctgccc tttttttacc ggggcgggaa tcggctgtat ggtttctaga atctgttctg
    22561 ccattttcgc ttccttatgt ggcagagtga tgtaggcttc tttgaggaag cctgcgcgga
    22621 cggttttttt gctgcctccg gaaatttgga tttgggcaac ggttttgcct ttcggatata
    22681 ttttgggcgt atcgaaggct tgcaatgccc agttcagcag cttgctgttg tctgaggcgc
    22741 gtgtttccgc cgattccgaa cctaatgtga tgacaaggat gtgcctgccg ttgccggagt
    22801 atgacacggc aaggttgtag ccgccgcttt ctgtgtgtcc ggctttcaga ccgtttacat
    22861 tgttgtccct atataaaagg atattgcggt tgttttgctc tatatttttg aatttgaaag
    22921 atttgatgga aaacagcggg taatattccg gaaagtcgcg catcaatgct tcagacagct
    22981 gggcgaggtc tttggcggtg gaaacctgtc cttctctact caagcctgtc gggtttttga
    23041 atacagtgtt cttcatgccc aagcgtcggg cttctttgtt catttgttgc acaaaatttt
    23101 caatcgagcc gttgcccagc cggccggcaa gggttagggc ggcatcgttt gcggatagtg
    23161 caatcatgcc ttttaagagt ttgtcggtgc tgaccgtatc gccgggacgt acaaacattc
    23221 tgcttccttc tgaagcccat gcggattcgg gtatttttaa gttttcttca gattggatat
    23281 ttcccgattt catgtttttg aaaaccaaat atgcggtcat cagttgggtt agtgccgccg
    23341 gttcgacagg ggtattgatg tttttggcgg ataaagtctg tctgctttga aggtcgataa
    23401 cgatgtgtgc cgctgtgagg gtttcgggtg tttggaacgt gggggcggcg tgtaccgccg
    23461 gtttgttggg cgcgggcgat gcggccgttg cgtgagaaac gcctaagatg atggaaagca
    23521 ggacgggcag gattttatgt gctgtcatga aatattctaa ttgtgtgcgt gtttcagtct
    23581 gccgattata cgcttagggt gtctgatcgg gcggattttt cttgctttcg cgccgtcttg
    23641 ggcgtatggt tttgggtttt gcgattttaa taaaccgatt atcccatatt gaattatgaa
    23701 cacgcccctt ccttattcca attacctcat ccgcatcctg acggcatctg tctatgatgt
    23761 cgcggtcgaa acgcctttgg aaccggcacg cagcctttct gtacgtttga aaaacaacat
    23821 ccttttgaaa cgcgaagatt tgcagccggt tttttcgttc aaaatacgcg gcgcgtacaa
    23881 caaaatgtcc aagttgccga aagatgcgct cgcttgcggc gtgattgcgg caagcgcggg
    23941 caatcatgct caaggcgtgg cattgtccgc acagcgtttg ggctgccgtg ccgttatcgt
    24001 tatgccggag actacgccga aaatcaaagt ggatgcggtt aagagccgtg gcggcgaggt
    24061 ggttttgcgg ggcgtttcat acaacgatgc ctacgattat gcgatggagt tggcggaaaa
    24121 agaagggtta acctatatcg cgccgtttga tgatcctgat gtgattgcgg gacaggggac
    24181 ggtggggatg gaaattgtca gccagcatcc cgatccaatc cgcgccgtat tcgtaccgat
    24241 aggcggtggc ggtttggcgg cgggcgtggc ggcatttatc aagcaggtcc gtcccgaaat
    24301 caaagttatc ggcgttcaga ccaacgattc ctgctgtatg aagcagtcgg tcgaagcggg
    24361 tgaaatcgtc catttgaaag atgtcgggct gttttcagac ggcactgcgg tcaaagtcgt
    24421 cggaaacgaa accttccgtc tctgcaaaga acttttggat gaaatcatta cagtcgatac
    24481 cgatgcggtt tgcggcgcgg tcaaggatat tttcgatgac acgcgcagca ttaccgagcc
    24541 ggcgggcgcg ttggcgttgg cgggtctgaa agcctatatc gcccgagaag gcgcggaaaa
    24601 ccaaaccctg attgccgtta ccagcggtgc gaatatgaat tttcaccgtt tgcgccacgt
    24661 ttcggaacgg agcgaattgg gcgagggcaa cgaaggtatt tttgccgtta ccatccccga
    24721 agagcgcggc agcttcctta agtttgtcaa tatattggga aataggaata ttaccgagtt
    24781 caactaccgc tacggggacg atgaaaaagc gcatatcttt gtcggacttc aagcggcagg
    24841 cccgcaggat ttggcggtta tcggcagccg gttggatgag gcgggattgc ccaatgtcga
    24901 cctgaccgat gatgagattg ccaaaatcca tatccgctat atggtcggag ggcggacgga
    24961 caaagtagaa aacgagcgtc tggtcagttt tgagtttccg gagcgtccgg gggcgttggc
    25021 gcgttttttg aaccatatgc agggcggttg gaacattacg ctcttccatt accgcaacca
    25081 cggtgcggat tacgggcgga ttttggtcgg tatcgatgtg ccgccgcacg atgccgccgc
    25141 atttgacggt ttcttggaaa gtctgggata cagctatcac gaggaaacgc aaaatgccgc
    25201 gtacaagctg tttcttgcct gacgcttgaa agcacaatgc cgtctgaaag cctttcagac
    25261 ggcattgcgc tttcatggtt aaatcgaata ttcaatcagt tcgttttgag agaagacata
    25321 aacctgtttc ggaatcagct tcaattcttt gccttcggcg attgggtaac gcgcggcatc
    25381 gctgccagcc agcgtgatat gcacgtcttg tttgccgtgt ttcaccaaaa tatgcgtcaa
    25441 tgcgccgacg gcgtggactt tttcgatttc ggcacaaatc atcggtgttt cgtgttcggc
    25501 ggcgatctgc cattcgtgcg ggcggatata gccggtggcg gtttgttcct gccatttgta
    25561 ttgtgcgtcc aatttccacg cgaagccgtt ataatgccag aagccttttt cgatgcgtcc
    25621 ttcaaaagcg tcggtttcgc cgaggaactc ggtaacgaag gcgttttcgg gtttgcggta
    25681 aatggcttcg gcactgccgg tttgttcgat tttgccgtgg ttcatcacga cgatttcgtc
    25741 ggaaacttcg agggcttctt cttggtcgtg cgtaaccaga atgctggtta cacccaggtt
    25801 gtgatggatg tcgcgcagcc aggtgcgtaa ttctttgcgt actttggcat ccaacgcgcc
    25861 gaagggttcg tccaaaagca agagtttggg ttcgaccgca agcgcgcggg cgagggcgat
    25921 gcgctggcgt tgcccgccgg agagttggtg cggataggat tttgccaaat gagagagctg
    25981 cacgagcttg agtaattctt cgactttggc gcggatttgt cctttggacg ggcgttcgga
    26041 cttgggcaat acggtcaaac cgaaagcgac gttgtcaaac acgttcatgt ggcggaagag
    26101 ggcgtagtgt tggaacacga agccgacttt gcgctcgcgc acgtgtttgg cggttacgtc
    26161 ttgcccgtca aagaggattt ggccgccgtc ggcgttttcc agtccggcga taatgcgtaa
    26221 aagcgtggtt ttgccgcagc cggacgggcc gagtagggaa acgagtttgc cggtggggac
    26281 gttgaggttg atgtttttca gcgcgtgaaa attgccgaag tgtttgttta agttttggat
    26341 ggtgatactc atactgtgtt cctttcggcg gcggcgagtt ttttgtcttg taatttggta
    26401 atgatgtttt gcaccgccag cgtcgccagt gctaaaagtg ccaatacgcc ggagagggcg
    26461 aatgcgccgg taaagttgta ttcgttgtag aagatttcga ccaaaagcgg gacggtgttg
    26521 gtttcgccgc gtatgtgtcc cgataccacg ctgaccgcgc cgaattcgcc catcgcgcgg
    26581 gcgttggtga ggatgatgcc gtagagtaac gcccatttga tgttgggcag ggtaacgcgc
    26641 caaaacatct gccagccgct tgcgccgagt atcagcgcgg cctgttcttc gctgtcgccc
    26701 tgcgcctgca ttaaaggaat gatttcacgc gcgacaaagg ggaaggtaac gaacaaggtc
    26761 gccaaaataa tgcccggaat ggcaaagata atctgtatgc cttgcgcttc gagccggcca
    26821 cccaatgccg tgtgcgcgcc gaacaataag acgaacatca aaccggccac cacgggcgat
    26881 acggaaaacg gcaaatcgag cagggtagtc agcaactgct tgccgcgaaa atcaaaacgg
    26941 gtcagcagcc acgccatcgc cacgcccaag acggcattga cggggacgac aatcagcgcg
    27001 gtaatcagcg tcaatttgat ggcagaccac gcttcgggat cgtttaagga tttcaggtac
    27061 aaatcccaac cgccttttaa ggcttcgtaa aacacggcga cgagcggcac gaccagcatc
    27121 agcagcagaa agcccagcgc ggcggcaatc agcaacacgc gcagccagcg cggttcggtc
    27181 aggttgggat tggcggaata gggtttcatg gcggtggtgt tccccggttg gtttaaaatg
    27241 tttggaggtc gtctgaaaag ggattgaggg gtttcagacg acctttttgt tcattaagta
    27301 gtaggtcgga tacttgtatc cgacaaggtt gctgaatcgc aaatgtttgt cggatacaag
    27361 tatccgacct acgaattctg tgttttgtgg ggcggattcg ttttcagacg accttttatt
    27421 gattaaggga ataaaaaaac gtctagctgc cgtcattccc gcgcaggcgg gaatccacca
    27481 cagggcaaca ggaaaacaga aaataaataa ggcagccgaa attcaccaat ggattcccgc
    27541 ctgcgcggga atgacggtaa caggtatttc agacgacctc aacccttcgc gcccgaacgc
    27601 ctgcccaacg cccactgcat cacgttcagc gcaaacagaa tcacaaacga aaccagcagc
    27661 ataaacaacg ccaccgccga cgcgccctgt acgtcgaact gttccagctt gcccgtaata
    27721 atcagcggca ggatttcaga aaccatcgga atattgcccg cgataaaaat caccgagccg
    27781 tattcccccg ttgcccgcgc gaacatcatt cccgcgccgg tcaagagtgc cggcgtgatt
    27841 tccggcagca gcacgcggcg gaacgtcgta aagcggtttg cgcccaaagt cgccgccgct
    27901 tcctcatatt cgcccgataa ttcctccaac accggctgca cggcgcggac gataaagggc
    27961 aggctgacga cgaccagcgc aatccaaatg ccgacgggcg taaacgcgat tttgatgccc
    28021 aaaggctcga aaaaacggcc tatccaaccg ttgggcgcat acagggttgc caacgcgata
    28081 cccgtaaccg ccgtcggcag cgcaaacggc aaatcaacca gcgcgttcgc cagacccttg
    28141 cccggaaatt catagcgcac caatacccac gccaccagcg tgccgaacac gacattggtc
    28201 agcatcgcat aaaacgacat ccgcaaactt agccacaccg ccgccaacac gttcggctcg
    28261 gtaatcgtgt tccaaaatcc gctccagccg atttccgccg ccttcgccgc catcatcgca
    28321 aacggcaaga ccacaagcag cgacaggcac aatacggtca gaccaaggct gagtttgaag
    28381 ccgggcagta cgccgggcgt tttgagcgct aacataaaac aatgctgaaa ataaggaaaa
    28441 ggaaggacta ctttaacgat gccgtccgaa aaacggaaag aatggaaagt ttggtgcaaa
    28501 gacgaatttg ttataaagcg gttggcagtt tctcaagcgg gcgcgatgtt ttaaaatata
    28561 gtggattaaa tttaaatcag gataaggcga cgaagccgca gacagtacaa atagtacggc
    28621 aaggcgaggc aacgccgtac tggtttaaat ttaatccact ataacgcctt gttttgacgg
    28681 aaaaccatca taataaagga acacttatgc agattttatc ttttcagccg gacattgcgg
    28741 aacgtatgct ggaaggtacg gaaggcgagt cggtcaacga aaacgcacaa ttcgtccgta
    28801 cggacaacgg ctattggatt gcgtggcatg aaggcgtagc ggcactgctt gcgcccgata
    28861 cgccgccggg cattccctgt ttttgggtgg aaggcgcgga aagccttgaa gagttgtgcg
    28921 tcatggtgga acgcggcgag tttgacgaag tggaagagtt tgacggcgat gacgacgaat
    28981 ggctcgaaac ggcacagggt tgcgggcacc acggcgacgc ttgcgcctgc ggacattaaa
    29041 ggcattgcag gcttgccgca aggggggcaa ggctttgccg ttttttaaat aatcggtgga
    29101 ttgcatctgt ttgcaatggg ctgaacgctt ccctttgtat tttattgaat aaaaaaactt
    29161 atcttggtat tataattaag gcagcatcaa ttattttggg atggcaataa acgcaaagca
    29221 ttgatttgcg ccgattgcag acttattata gcaggttgcg gcgcggactt aatgggaatt
    29281 gtttatagag catgatggtc tgaaacatca ttaattaaaa ataccattaa tccgatttat
    29341 atttatttca atttcaatgg aaaaacatca atgacaatga ttttaagcat tttaagcctg
    29401 ttttttatca tcagactgtt atttttagcc gtctctatta aacatgaaaa agccttgatt
    29461 gccaaagggg caaaacaata cggaaaaacc aattccacgc tgcttgcggc agttcatacg
    29521 ctttattatt tggcgtgttt tgtttgggta tggctttctg acactgcttt taatggcata
    29581 tccttgattg gtacgctgac ggtgatggct tcatttgtga tattgtcatt gattattaag
    29641 cagttggggg agatttggac ggttaaaatc tatattttac caaatcatca aattaatcgt
    29701 tcgtggttgt ttaaaacatt ccgccacccc aattattttt taaacatcat acccgaactg
    29761 attggcatcg ccttattatg tcaagcgtgg tatgttttat tgattggtct gcccatttat
    29821 ttgctggtct tatttaagcg tatccgacaa gaagaacagg cgatggcaac acttttttaa
    29881 cccgtttcat caattatagc ggattaacaa aaaccagtac ggcgttgcct cgcct
//
LOCUS       DAXHSF010000005        29163 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.005, whole genome shotgun sequence.
ACCESSION   DAXHSF010000005 DAXHSF010000000
VERSION     DAXHSF010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 29163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29163
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.005"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(92..880)
                     /locus_tag="ACFM8S_000170"
     CDS             complement(92..880)
                     /locus_tag="ACFM8S_000170"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6313309.1"
                     /translation="MNHQDARWQVYRHLAEHADQRLTLVRNAPKHILLAGADADISRS
                     LLAKRYPQAVFEEYDSRADFLAAAAAARKGGFWQRFAGKGVVQHCQSPTAPLPEACAD
                     MLWSNLGLLAAESIVPVLQNWARALKTDGLLFFTCFGRDTLAELKCRLKENGIESRSV
                     MFPDMHDLGDMLAENGFYDPVTDTAKLVLDYKKAETFWADMDTLGVWRAMSWNDENAA
                     RSCVGTIFEREGGLSITLETVYGHAVKKLMLPQGENVVQFFPKR"
     gene            complement(885..1625)
                     /gene="bioH"
                     /locus_tag="ACFM8S_000171"
                     /pseudo
     CDS             complement(885..1625)
                     /gene="bioH"
                     /locus_tag="ACFM8S_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
     gene            1624..2349
                     /locus_tag="ACFM8S_000172"
     CDS             1624..2349
                     /locus_tag="ACFM8S_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFC6313310.1"
                     /translation="MDFLSCWRRIADAPTIRRCVLCHGSSGVSDGICAGCRNDLAAYR
                     TDAANSCPLCFRHVQGGAVCGGCQKKPPAFDRMWASLHYEPPVNNMIRALKHLADLGM
                     VQPLADLMMQNPPDRLADECFDFVLPVPLSRERLLQRGFNQSEGIVGLLAQRYGWRIL
                     PRHTVFRHHRPPQSTLKGGERRRNIKNAFEIRTPPPKNCNILLIDDVFTTGATLDELA
                     KTLKKSGANRICCWTLARTPMKK"
     gene            2414..2878
                     /gene="trmL"
                     /locus_tag="ACFM8S_000173"
     CDS             2414..2878
                     /gene="trmL"
                     /locus_tag="ACFM8S_000173"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFC6313311.1"
                     /translation="MFTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKM
                     KRAGLDYHEFASLTVHENFDDCLKALAGRRIFALTTKGTARPDETAFQKGDVLLFGPE
                     TRGLPADILDSLPAAQKIRLPMQPGSRSMNLSNTVAVILFEAWRQHGYAGGV"
     gene            2889..3011
                     /locus_tag="ACFM8S_000174"
     CDS             2889..3011
                     /locus_tag="ACFM8S_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFC6313312.1"
                     /translation="MPSENLSGHIPNRRPHCTAVRNVSGGHDAHSSSTQERNTF"
     gene            3008..3727
                     /locus_tag="ACFM8S_000175"
     CDS             3008..3727
                     /locus_tag="ACFM8S_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HFC6313313.1"
                     /translation="MTLTRKTLFLLTAAFGTHSLQTASADAVVKAEKLHASANRSYKV
                     AGKRYTPKNQVAEFTQTGNASWYGGRFHGRKTSGGERYDMNAFTAAHKTLPIPSYVRV
                     TNTKNGKSVIVRVNDRGPFHGNRIIDVSKAAAQKLGFVNQGTAHVKIEQIVPGQSAPV
                     AENKDIFIDLKSFGTEHEAQAYLNQAAQNFAVSSSGTNLSVEKRRYEYVVKMGPFASQ
                     ERAAEAEAQARGMVRAVLTAG"
     gene            3781..4107
                     /locus_tag="ACFM8S_000176"
     CDS             3781..4107
                     /locus_tag="ACFM8S_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFC6313314.1"
                     /translation="MNFKRLLLTAAATALMGISAPALAHHDGHGDDDHGHAAHQHSKQ
                     DKIISRAQAEKAALARVGGKITDIDLEHDNGRPHYDVEIVKNGQEYKVVVDARTGRVI
                     SSRRDD"
     gene            4189..4773
                     /gene="ruvA"
                     /locus_tag="ACFM8S_000177"
     CDS             4189..4773
                     /gene="ruvA"
                     /locus_tag="ACFM8S_000177"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFC6313315.1"
                     /translation="MISRLTGKLVEKNPPQIVIDVNGVGYEADVSMQTFYNLPPVGES
                     VQLFTQLIIREDAHLLFGFATAEERKTFRQLIKVGGIGAKTALGILSAMTADELAQAV
                     AEEDVKRLSSAPGIGKKTAERMVLELRGKLVAHAVTDGLFAAAPAADETEDIVGTLLA
                     LGYSEREAKAAVKGVPEGTDVGEGVRLALKNLLK"
     gene            4773..4910
                     /locus_tag="ACFM8S_000178"
     CDS             4773..4910
                     /locus_tag="ACFM8S_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313316.1"
                     /translation="MPPEGGAAFALTKPCVSASRCLPDGFSRKMALLSFLSDGIVFDA
                     V"
     gene            4936..6792
                     /locus_tag="ACFM8S_000179"
     CDS             4936..6792
                     /locus_tag="ACFM8S_000179"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC6313317.1"
                     /translation="MLNKIFSWFESRIDPYPEAAPKTPEKGLWRFVWSSMAGVRKWIA
                     ALAALTAGIGIMEALVFQFMGKIVEWLGKYAPAELFAEKGWELAAMAAMMVFSVVWAY
                     AASNVRLQTLQGVFPMRLRWNFHRLMLNQSLGFYQDEFAGRVSAKVMQTALALRDAVM
                     TVADMVVYVSVYFITSGVILASLDSWLLLPFIGWIVGFASVMRLLIPKLGQTAAWQAD
                     ARSLMTGRITDAYSNIATVKLFSHGAREAAYAKQSMEEFMVTVRAQMRLATLLHSCSF
                     IVNTSLTLSTAALGIWLWHNGQVGVGAVATATAMALRVNGLSQYIMWESARLFENIGT
                     VGDGMATLSKPHTILDKPNALPLKVSQGEIKFEHIDFSYEAGKPLLNGFNLTIRPGEK
                     VGLIGRSGAGKSTIVNLLLRFYEPQSGTVLIDGQDISGVTQESLRAQIGLVTQDTSLL
                     HRSVRDNIIYGRPDATDAEMVSAAERAEAAGFIPDLSDAKGRRGYDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQESLDKMMDGKTVIAIAHRLST
                     IAAMDRLVVLDKGRIIEEGTHAELLEKRGLYAKLWAHQSGGFLNEHVEWQHD"
     gene            6797..7720
                     /gene="rsgA"
                     /locus_tag="ACFM8S_000180"
     CDS             6797..7720
                     /gene="rsgA"
                     /locus_tag="ACFM8S_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFC6313318.1"
                     /translation="MPSEHPFSDGISTPNPKETMNDTAQITASYGRRYIVRTPDGTTY
                     EASTRKKRVDFACGDRVRISPVNAEQVVIEDFLPRQSLLYRQDAWKTKLIAANVTQLL
                     IVTAAVPSPSMRLLQRALLAAEAAGIRAVIVLNKADLPETALWREKLKFYETLGYPVI
                     ETRALENADILRPVLQGHSNILLGQSGMGKSTLTNALLGSQTARTGDISAALDSGKHT
                     TTHARLYDLNGETQLIDSPGLQEFGLHHLQAADLPRYFPDFRHLVGQCRFHNCTHRAE
                     PGCAFKAAAETRAASPERLAFLQGITDELPG"
     gene            7747..8058
                     /locus_tag="ACFM8S_000181"
     CDS             7747..8058
                     /locus_tag="ACFM8S_000181"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFC6313319.1"
                     /translation="MPSEAGFGFQTASVLQKCYNPLFTGTSEAMKKNAPKSFEEALSR
                     LESLTQSMQGEMPLEDALAAYQEGNELVRYCQTKLAQVEQKLQVLDADGPKELNLESD
                     E"
     gene            8042..8938
                     /locus_tag="ACFM8S_000182"
     CDS             8042..8938
                     /locus_tag="ACFM8S_000182"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFC6313320.1"
                     /translation="MNPTNDLKAWQQRAQAQTELLLERFLPSENEIPHTLHEAMRYAA
                     LDGGKRLRPMLVLAASELGGTVADAVGQAMAAIEMIHVYSLVHDDMPAMDNDSLRRGK
                     PTCHIKYGEATALLVGDALQTQAFDVLARPSGLPASRQLAMLSVLAKAGGSRGMAGGQ
                     AIDLANVGKQMVQADLEQMHSLKTGALIRAAVLLGAMSCPNLSEDSLAVLDDYAAKLG
                     LAFQVIDDVLDCEADTATLGKTAGKDADNDKPTYVKLMGLEAARSYAHKLVAEAVALL
                     EPFGDKALRLRQLAEFAVARKY"
     gene            complement(9047..9346)
                     /locus_tag="ACFM8S_000183"
     CDS             complement(9047..9346)
                     /locus_tag="ACFM8S_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HFC6313321.1"
                     /translation="MTASMYILLLLALIFANAPFLTTRLFGIVPLKRKHFGHHLIELA
                     AGFALTAVLAYILESRAGAVHDQGWEFYATVVCLYLIFAFPCFVWRYFWHTRNRE"
     gene            complement(9458..10903)
                     /gene="nuoN"
                     /locus_tag="ACFM8S_000184"
     CDS             complement(9458..10903)
                     /gene="nuoN"
                     /locus_tag="ACFM8S_000184"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFC6313322.1"
                     /translation="MNWSDLNLMPAMPEVALLSLLVLLLLADLWVSDDKRRWTHYGAL
                     ATVAVTAVVQLAVWEQGSTSSFNGMYIADGMSRLAKMVLYALTFALFVYAKPYNQVRG
                     IFKGEFYTLSLFALLGMSVMVSAGHFLTAYIGLELLSLALYALIALRRDSGFAAEAAL
                     KYFVLGALASGLLLYGISMVYGATGSLEFAGVLASSFNEEANEWLLKLGLVFIVVAVA
                     FKLGAVPFHMWVPDVYHGAPTSVTALVGTAPKVAAVVFAFRILVTGLGTVHHDWSLMF
                     ALLAAASLLVGNLAAIMQTNIKRMLAYSTVSHMGFILLAFMAGAVGFAAGLYYAITYA
                     LMAAAGFGVLMVLSDGDNECENISDLAGLNQHRVWLAFLMLLVMFSMAGIPPLMGFYA
                     KFGVIMALLKQGYVWLSVFAVVMSLIGAFYYLRVVKVMYFDESGRARPTVGGNNAAKF
                     LLSVNALLLVLWGIMPQTVIDWCAKALENTL"
     gene            complement(10913..12409)
                     /locus_tag="ACFM8S_000185"
     CDS             complement(10913..12409)
                     /locus_tag="ACFM8S_000185"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFC6313323.1"
                     /translation="MFSNYLLSLAIWIPIAAGVLVLATGKDSRAPLARVLAFMGALAG
                     FLVTLPLFTGFDRLSGGYQFTEFHEWIPLLKINYALGVDGISVLFIILNAFITLLVVL
                     AGWEVIQKRPAQYMAAFLMMSGLINGAFAAQDAILFYVFFEGMLIPLYLIIGVWGGPR
                     RVYASVKLFLYTLMGSLLMLVAMVYLYYQTGSFSIVDFQNIKQIPLGVQQLLFAAFFL
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSMVLAAITLKLGAYGFLRFILPIMPDAARYFA
                     PVIIVLSLIAVIYIGMVALVQTDMKKLVAYSSISHMGFVTLGMFLFVDGQLDDWALKG
                     AVIQMISHGFVSAAMFMCIGVMYDRLHTRNIADYGGVVNVMPKFAAFMMLFGMANAGL
                     PATSGFVGEFMVIMGAVKVNFWVGALAAMTLIYGASYTLWMYKRVIYGAIHNPHVAEM
                     KDINCREFAILAILAVVVLGMGLYPQAFIEVVHQAANDLIAHVAQSKI"
     gene            complement(12522..13685)
                     /locus_tag="ACFM8S_000186"
     CDS             complement(12522..13685)
                     /locus_tag="ACFM8S_000186"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease PLD domain-containing
                     protein"
                     /protein_id="HFC6313324.1"
                     /translation="MTQLFLHSDTPNDKKLISIYEKAVSEAVELYIFSAYLTHWDVKN
                     KLNSKLQEFKFIFGQDFGLSKKEAIQEVLKWLPSHLKFTLMVAQGIQGFHPKAVFWKN
                     DKNEYYALIGSSNLTHAAFNSNYEANILTKISEQDFIKAKSWANEIVMKSIPVSEDWL
                     EEYQEAEINYKKSTVRQSVMDKLFMEMPNYNQELIAARRKQMRNHQTVCNQLKNLIKQ
                     CAAGKIDNNDFYGEFNKLWSWKSENKGEGVGNRFQDKTWKRTGKSSDFRKLCIAIQSV
                     FDAPLTERDNVVAKQIDWLKECGVSTRGSVFSEMLCQEYPDRYPVLNAPIKKFLEENK
                     FKSAKGASEGSKYIDLSMKLRALLAMQSEIKDLAELDVLVQAEYRNRTDIDWE"
     gene            complement(13744..14064)
                     /locus_tag="ACFM8S_000187"
     CDS             complement(13744..14064)
                     /locus_tag="ACFM8S_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HFC6313325.1"
                     /translation="MSNTQTIRSADFTASRAWGALDIANMNGTTVRLHWTDQSYKWHV
                     NDGEEVFAVMDGEVDMHYRENGEEHIVRLKSGDIFYAGIGTEHVAHPCGEARILVIEK
                     EGSA"
     gene            complement(14132..16156)
                     /gene="nuoL"
                     /locus_tag="ACFM8S_000188"
     CDS             complement(14132..16156)
                     /gene="nuoL"
                     /locus_tag="ACFM8S_000188"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFC6313326.1"
                     /translation="MNDMTLYLIIALVPLAGSLIAGLFGNKIGRAGAHTVTILGVAVS
                     AVLSAYVLWGFLNGSRAKFDENVYTWLTMGGLDFSVGFLVDTMTAMMMVVVTGVSLMV
                     HIYTIGYMHDEKVGYQRFFSYISLFTFSMLMLIMSNNFIQLFFGWEAVGLVSYLLIGF
                     YFKRPSATFANLKAFLINRVGDFGFLLGIGLVLAYFGGSLRYQDVFAYLPNVQNATIQ
                     LFPGVEWSLIIVTCLLLFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGL
                     FMVSRMSPIYEMSSTALSVIMVIGAITALFMGFLGVIQNDIKRVVAYSTLSQLGYMTV
                     ALGASAYSVAMFHVMTHAFFKALLFLAAGSAIIGMHHDQDMRHMGNLKKYMPITWLTM
                     LIGNLSLIGTPFFSGFYSKDSIIEAAKYSTLPGSGFAYFAVLASVFVTAFYAFRQYFM
                     VFHGEEKWRSLPEHHDDHHGEEHHSLGKNDNPHESPLVVTLPLILLVIPSVIIGYVAI
                     EPMLYGDFFKDVIFVNTDAHPTMHIMKEEFHGALAMVSHSLHSPVLYLAIAGVLSAWL
                     LYVKLPHLPAKIAQAFRPVYVLFENKYYLDALYFNVFAKGTRALGTFFWKVGDTAIID
                     NGIVNGSAKLVGAVAAQVRKAQTGFIYTYAAAMVFGVLVLLGMTFWGLFR"
     gene            complement(16205..16546)
                     /locus_tag="ACFM8S_000189"
     CDS             complement(16205..16546)
                     /locus_tag="ACFM8S_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313327.1"
                     /translation="MMDKNQLEQEFHKAMLNIYQEALNLPQPYKATRFLQIVNEFGGK
                     EAADKLLSTGEKKTQTGFTELILSGGGVHALKYSMEYLVLQKPWCDLFTEEQLAVARK
                     RLERVGFVSPK"
     gene            complement(16670..17245)
                     /gene="fic"
                     /locus_tag="ACFM8S_000190"
     CDS             complement(16670..17245)
                     /gene="fic"
                     /locus_tag="ACFM8S_000190"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFC6313328.1"
                     /translation="MPSENPIGKTMKSIDEQSLHNARRLFESGDIDRIEVGTTAGLQQ
                     IHRYLFGGLYDFAGQIREDNISKGGFRFANAMYLKEALVKIEQMPERTFEEIIAKYVE
                     MNIAHPFLEGNGRSTRIWLDLVLKKNLKKVVNWQNVSKTLYLQAMERSPVNDLELRFL
                     LKDNLTDDVDNREIIFKGIEQSYYYEGYEKG"
     gene            complement(17279..17584)
                     /gene="nuoK"
                     /locus_tag="ACFM8S_000191"
     CDS             complement(17279..17584)
                     /gene="nuoK"
                     /locus_tag="ACFM8S_000191"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFC6313329.1"
                     /translation="MITLTHYLVLGALLFGISAMGIFMNRKNVLVLLMSIELMLLAVN
                     FNFIAFSQHLGDTAGQIFVFFVLTVAAAESAIGLAIMVLVYRNRQTINVADLDELKG"
     gene            complement(17581..18252)
                     /locus_tag="ACFM8S_000192"
     CDS             complement(17581..18252)
                     /locus_tag="ACFM8S_000192"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFC6313330.1"
                     /translation="MTFQLILFYIFAVIILYGAIKTVTAKNPVHAALHLVLTFCVSAM
                     IWMLMQAEFLGVTLVVVYVGAVMVLFLFVVMMLNIDIEEMRAGFWRHAPVAGVVGTLL
                     AVALILILVNPKTDLAAFGLMKDIPADYNNIRDLGSRIYTDYLLPFELAAVLLLLGMV
                     AAIALVHRKTVNPKRMDPADQVKVRADQGRMRLVKMEAVKPQTESAEESEVSDDLKPK
                     EEGKA"
     gene            complement(18288..18995)
                     /locus_tag="ACFM8S_000193"
     CDS             complement(18288..18995)
                     /locus_tag="ACFM8S_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313331.1"
                     /translation="MEGLINALKYLAEHEPIDNFEEIRTRNSPIELPSGLSNFEQNIF
                     LKENLSPKLQNDDSLKTHYWIIREWGGIKSFKQSAENSQLIRQFLSELNSGKLSSGLL
                     KISSLSKLASFIDCERFAIYDSRAIFSLNWLLFKFTNADLFFQPQGRNRELEIRNMNV
                     LFHFSDIKPNYRKPDVSFHQYCGLLQDLAKQVYGKQAKPYHIEMLLFKIATTWICADM
                     DQLIKFDCLRNQDFQTA"
     gene            complement(19026..19505)
                     /gene="nuoI"
                     /locus_tag="ACFM8S_000194"
     CDS             complement(19026..19505)
                     /gene="nuoI"
                     /locus_tag="ACFM8S_000194"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFC6313332.1"
                     /translation="MANLVKTFLLGELVKGMGVTLKNFFARKDTIYFPEEKTPQSVRF
                     RGLHAQRRYPNGEERCIACKLCEAVCPAMAINIESEEREDGTRRTKRYDIDLTKCIFC
                     GFCEEACPTDAIVETHIFEYHGEKKGDLHMTKPILLAIGDKYEAEIAKRKAADAPYR"
     gene            complement(19586..20662)
                     /gene="nuoH"
                     /locus_tag="ACFM8S_000195"
     CDS             complement(19586..20662)
                     /gene="nuoH"
                     /locus_tag="ACFM8S_000195"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFC6313333.1"
                     /translation="MQEWFQNLFAATLGLGDLGITVGLVVSVIVKIVIILIPLILTVA
                     YLTYFERKVIGFMQLRVGPNVTGPWGLIQPFADVFKLLFKEVTRPRLSNKALFYIGPI
                     MSLAPSFAAWAVIPFNEEWVLTNINIGLLYILMITSLSVYGVIIAGWASNSKYSFLGA
                     MRASAQSISYEIAMSAALVCVVMVSGSMNFSDIVAAQAKGIAGGSVFSWNWLPLFPIF
                     IVYLISAVAETNRAPFDVAEGESEIVAGHHVEYSGFAFALFFLAEYIFMILIAALTSL
                     MFLGGWLSPFPQSWGFIGTPSAFWMFVKMAAVLYWYLWIRATFPRYRYDQIMRLGWKV
                     LIPIGFAYIVVLGVWMISPLNLWK"
     gene            complement(20665..22926)
                     /gene="nuoG"
                     /locus_tag="ACFM8S_000196"
     CDS             complement(20665..22926)
                     /gene="nuoG"
                     /locus_tag="ACFM8S_000196"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFC6313334.1"
                     /translation="MLQIEIDGKQVSVEQGATVIEAAHKLGTYIPHFCYHKKLSIAAN
                     CRMCLVNVEKAPKPLPACATPVTDGMIVRTHSAKAREAQEGVMEFLLINHPLDCPTCD
                     QGGECQLQDLAVGYGKTTSRYTEEKRSVVGKDMGPLVSAEEMSRCIHCTRCVRFTEEI
                     AGLQEIAMVNRGEHSEIMPFIGKTVETELSGNVIDLCPVGALTSKPFRFNARTWELNR
                     RKSVSAHDALGSNLIIQTKDHTVRRVLPLENEAINECWLSDRDRFAYEGLYHESRLKN
                     PKIKQGGEWMDVDWKTALEYVHSAIKCIAKDGNQNQVGVWANPMNTVEELYLAKKLAD
                     GLGVKNFATRLRQQDKRLSDGIKGAQWLGQSIESLADNDAVLVVGANLRKEQPLLTAR
                     LRRAAKDRMALSVLASSKEELFMPLLSQEAVHPDEWAGRLKNLSADAEHAVTASLKNA
                     EKAAVILGAEVQNHPDYAAIYAAVQELADATGAVLGILPQAANSVGADVLGVNSGESV
                     AEMANAPKQAVLLLNVEPEIDTVDGAKAVAALKQAKSVMAFTPFVSETLLDVCDVLLP
                     IAPFTETSGSFINMEGRLQSFHGVVQGFGDSRPMWKVLRVLGNLFDLKGFEYHDTVAI
                     LKDALDAESLPSKLNNRAASTQKDFQTASSRLVRVGGVGIYHTDAIVRRSAPLQETSH
                     AAVPAARVNPNTLARLGLQDGQTAVAKQNGASVSVAVKADAGLPENVVHLPLHTENAA
                     LGALMDTIELAGA"
     gene            complement(23216..23653)
                     /locus_tag="ACFM8S_000197"
     CDS             complement(23216..23653)
                     /locus_tag="ACFM8S_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313335.1"
                     /translation="MVEAKIFILYGAANKGKSTTLNTLFNQICRKFSKFLVFFERHGN
                     GLDFVAVFDHEGQRIGFYSSGDNEYEVRRNLYKLYSHNCDFIFGTSRTRGGSCDAVGC
                     YAELLHGDVNIINWCEKFEPTDEDNERAVKELFKSFKNIINKL"
     gene            complement(23653..24954)
                     /gene="nuoF"
                     /locus_tag="ACFM8S_000198"
     CDS             complement(23653..24954)
                     /gene="nuoF"
                     /locus_tag="ACFM8S_000198"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFC6313336.1"
                     /translation="MAIYQSGVIFDQVDTANPDCWTLDEYVKRGGYTALRKILSENIS
                     QTDVIDEVKTSGLRGRGGAGFPTGLKWSFMPRSFPGEKYVVCNTDEGEPGTFKDRDII
                     MFNPHALIEGMIIAGYAMGAKAGYNYIHGEIFEGYQRFEAALEQARAAGFLGKNILGS
                     DFEFELFAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTET
                     FSSVPFIIRDGGQAFADKGIPNAGGTKLFCISGHVERPGNYEVPLGTPFAEVLKMAGG
                     MRGGKKLKAVIPGGSSAPVLPADIMMQTNMDYDSISKAGSMLGSGAIIVMDEDVCMVK
                     ALERLSYFYYDESCGQCTPCREGTGWLYRIVHRIVEGKGRMEDLDLLDSVGKQMAGRT
                     ICALADAAVFPVRSFTKHFRDEFVHYIEHGRPMKEHKWGGW"
     gene            complement(24930..25052)
                     /locus_tag="ACFM8S_000199"
     CDS             complement(24930..25052)
                     /locus_tag="ACFM8S_000199"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313337.1"
                     /translation="MPSENRQTVSDGIRNIETAGSRNRYENEQAHQKWLFTNQA"
     gene            complement(25114..25689)
                     /locus_tag="ACFM8S_000200"
     CDS             complement(25114..25689)
                     /locus_tag="ACFM8S_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HFC6313338.1"
                     /translation="MRGVIVKWDDEKGYGFIRIDGGNKEIFCHISDFSPRNPRPEINE
                     AVGFEVVSDGKGRFSAKQIRYLNRERAVSPGYCKKHRKDEESPTSIVQVVLSLSVGLL
                     LVSVIGYKAYQYASEKLTPKQENTPVKTQVLPEIKRQQPDSSPYRCDGRQHCSQMTSC
                     EEAKWFARNCSDTKMDGDGDGIPCENMCGGW"
     gene            complement(25826..26299)
                     /gene="nuoE"
                     /locus_tag="ACFM8S_000201"
     CDS             complement(25826..26299)
                     /gene="nuoE"
                     /locus_tag="ACFM8S_000201"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFC6313339.1"
                     /translation="MLSAESLKQIDIELAKYPADQRRSAIMGALRIAQTEKGWLAPET
                     IAFVADYIGISPAQAYEVATFYNMYNLEPVGKYKLTVCTNLPCALRGGMATGEYLKQK
                     LGIGYGETTPDGKFTLVEGECMGACGDAPVMLVNNHSMCGFMTEEAIEKKLAELE"
     gene            complement(26299..27555)
                     /gene="nuoD"
                     /locus_tag="ACFM8S_000202"
     CDS             complement(26299..27555)
                     /gene="nuoD"
                     /locus_tag="ACFM8S_000202"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HFC6313340.1"
                     /translation="MANKLRNYTINFGPQHPAAHGVLRMILELDGEQIVRADPHIGLL
                     HRGTEKLAETKTYLQALPYMDRLDYVSMMVNEQAYCLAVEKLAGIDVPIRAQYIRVMF
                     AEVTRILNHLMGIGSHAFDIGAMTAILYAFRDREELMDLYEAVSGARMHAAYFRPGGV
                     YRDLPDFMPKYEGSKFRNAKVLKQLNESREGTMLDFIDAFCERFPKNIDTLETLLTDN
                     RIWKQRTVGIGVVSPERAMQKGFTGVMLRGSGVEWDVRKKQPYEVYDKMDFDIPVGVN
                     GDCYDRYLCRMEEMRQSVRIIKQCVDWLRVNPGPVITTNHKFAPPKRTEMKTGMEDLI
                     HHFKLFTEGMHVPEGETYTAVEHPKGEFGVYIISDGANKPYRLKIRAPGFAHLQGMDE
                     MAKGHMLADVVAIIGTQDIVFGEVDR"
     gene            complement(27545..28138)
                     /locus_tag="ACFM8S_000203"
     CDS             complement(27545..28138)
                     /locus_tag="ACFM8S_000203"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HFC6313341.1"
                     /translation="MASIQNLYETVSRVLGNQASKVISALGEITVECLPEHYISVMIA
                     LRDHEELHFELLVDLCGVDYSTYKNEVWQGKRFAVVSQLLSVKNNQRIRVRVWVSDDD
                     FPVVESVVDIYNSADWYEREAFDMYGIMFNNHPDLRRILTDYGFVGHPFRKDFPISGY
                     VEMRYDEEQKRVIYQPVTIEPREITPRIVREENYGGQ"
     gene            complement(28151..28633)
                     /locus_tag="ACFM8S_000204"
     CDS             complement(28151..28633)
                     /locus_tag="ACFM8S_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HFC6313342.1"
                     /translation="MGIEGVLKKGFITTSADTVLNYMRTGSLWPVTFGLACCAVEMMH
                     AGMARYDLDRFGIIFRPSPRQADLMIVAGTLTNKMAPALRRVYDQLAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGADRVVPVDVYVPGCPPTAEALIYGLIQLQQKIKRTSTIARDE
                     "
     gene            complement(28624..28980)
                     /locus_tag="ACFM8S_000205"
     CDS             complement(28624..28980)
                     /locus_tag="ACFM8S_000205"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HFC6313343.1"
                     /translation="MLSAYFPVFVFILVGLAAGVLFILLGTILGPKRHYAEKDAPYEC
                     GFEAFENARMKFDVRYYLVAILFILFDLEVAFMLPWAVVFKDLGAYGFWSMLVFIVVL
                     TVGFIYEWKKGALEWE"
BASE COUNT         7340 a         8063 c         7151 g         6609 t
ORIGIN      
        1 cactatacat cggtatttcc tttatttctg tcggttttca cagacaaatg ccgtctgaaa
       61 cctggaaacg gcttcagacg gcatctgcac atcatctctt cggaaaaaac tgcaccacgt
      121 tctccccttg cggcagcatc agttttttaa cggcgtgtcc gtacactgtt tccagcgtaa
      181 tactcaaacc gccttcccgt tcaaatattg tcccgacaca cgaacgcgcg gcgttttcat
      241 cgttccacga catcgcccgc caaacgccca gcgtgtccat atccgcccaa aacgtttccg
      301 cctttttgta atccaacacc agcttcgccg tatcggtaac ggggtcgtaa aacccgtttt
      361 cagcaagcat atcgcccaag tcgtgcatat cgggaaacat cacactgcgg ctttcaatgc
      421 cgttttcttt cagacggcat ttcagttccg ccaaggtatc gcgcccgaag caggtgaaaa
      481 acagcagccc gtccgtcttc agcgcgcgcg cccaattttg cagcacgggg acgatgcttt
      541 ccgccgccaa cagtccgaga ttcgaccaca acatatcggc acacgcttcg ggcagcggcg
      601 cggtcgggga ttggcagtgc tgcaccacgc ccttacccgc aaacctttgc caaaaaccgc
      661 ctttgcgcgc ggctgccgcc gccgccaaaa aatccgcacg ggaatcgtat tcttcaaata
      721 ccgcctgcgg atagcgtttc gccagcaggc tgcggctgat gtccgcatcc gccccggcaa
      781 gcaggatatg tttgggcgcg ttgcggacga gtgtcaggcg ttggtcggca tgttcggcaa
      841 gatggcggta aacctgccag cgtgcgtcct gatggttcat ctaaaacccc ttcaacaaag
      901 tcgcggtaca gcgcggcaaa cgcttccgcg tggctcaaaa acggcgcgtg tgccgccttt
      961 tccatcacaa ccagcctgct gcccttcaaa cggcggtgca gatactcgcc catgcgcggc
     1021 ggcgtaatcg cgtctttgcc gccgaacacc agcagtaccg gaacatctat cttgtccaac
     1081 aaatgccgcg catccgccct ttccgccgcg tccaaagctt cctgtaaggc ggagggcgtg
     1141 ccgcagcgcg ccaaatcggg caggattctg cctatgattc cgtccgcatc aggcgtgtgc
     1201 aaaagctgga gttgtagaaa ctgtttgata tgtttggcat aatccgttcg gaacgcgccg
     1261 accattttgc ccaatgcagg cgcggcaagc ccttcgggat aatcttcggc agccgtcagc
     1321 cgtgcgaaac tcgccgtcag gcagagcgaa cggactttgt cgggatggcg cgccgccaga
     1381 tacagcgcaa ccaatccgcc gagcgaccag ccgagaatgt cggcaggcgc gtcaatttgg
     1441 gcggcaatgc cgtctgccgc tgcctcaata tcgaagggtt gcgcgaaggg cgcgtcgccg
     1501 tgtccgggca aatcgacggc ggacaccggc cacgttgcag gcaggcgcgg catcaaatcg
     1561 tcgaacacgt ggcggttcgc cccccaaccg tgtatcaggt aaactttttt gacggcatca
     1621 ggcatggatt ttctctcttg ttggcggcgc atcgcagacg cgcccactat caggcgttgc
     1681 gtattatgcc acggttcgtc cggcgtttca gacggcatct gcgctggctg ccgcaacgat
     1741 ttggcggcat accgcaccga cgcggcaaac agctgccccc tgtgtttcag gcacgttcaa
     1801 ggcggcgcgg tatgcggcgg ctgtcagaaa aaaccgcccg cattcgaccg gatgtgggca
     1861 tcgctgcatt acgaaccgcc cgtcaacaat atgatacgcg cgctgaagca cttggctgat
     1921 ttgggcatgg tgcagccgct ggcagacctg atgatgcaga atccgcccga ccggcttgca
     1981 gacgaatgtt tcgatttcgt cctgcccgtt ccgctaagca gggagcggct gctgcaacgc
     2041 gggttcaacc aaagcgaggg catcgtcggg ttgttggcgc aacgctacgg ctggcggata
     2101 ctgccccgac acaccgtatt ccgacaccac cgtccgccgc aaagcacgct caaaggcggc
     2161 gaacggcggc gaaatatcaa aaacgccttt gaaatccgca caccgccgcc gaaaaactgt
     2221 aatattttgt taatcgacga tgtctttacc accggcgcga cgctggacga attggcaaag
     2281 acgctgaaaa aatcgggcgc aaaccgaatc tgctgctgga cgctggcacg cacgccaatg
     2341 aaaaaataac taaatttttt gacaccccat ccgccttgtg gcaaggttac gcgcctataa
     2401 gtgattgata tttatgttta ccatcgtttt ataccagccg gaaatcccgc cgaacacggg
     2461 caacatcatc cgcctgtgcg ccaataccgg cgcggatttg caccttgtca aaccgctcgg
     2521 cttcccattg gattccgcca aaatgaaacg cgccgggctc gactaccacg agttcgccag
     2581 cctgacggtg cacgaaaact tcgacgactg cctcaaggca ctcgcaggca ggcgcatttt
     2641 cgccctgacc accaaaggca cggcgcgccc cgatgaaacc gcgtttcaaa aaggcgacgt
     2701 tttactgttc gggccggaaa cgcgcggact gcctgccgac atcctcgaca gcctgcccgc
     2761 cgcgcaaaaa atccgcctgc cgatgcagcc cggcagccgg agtatgaacc tttccaacac
     2821 cgtcgccgtg attctctttg aagcgtggcg gcaacacggt tacgcaggcg gcgtttgaac
     2881 gcaggttcat gccgtctgaa aacctatccg gacacattcc gaaccgccgt ccgcactgta
     2941 cggcggttcg gaacgtatcc ggcgggcatg atgcccattc gtcttcaact caagaacgga
     3001 acacgttttg actttaaccc gaaaaaccct tttccttctc accgccgcat tcggcacaca
     3061 ctcccttcag acggcatccg ccgacgcagt ggtcaaggca gaaaaactgc acgcctccgc
     3121 caaccgcagc tacaaagtcg ccggaaaacg ctacacgccg aaaaaccaag tcgccgaatt
     3181 cacgcaaacc ggcaacgcct cgtggtacgg cggcaggttt cacgggcgca aaacttccgg
     3241 cggagaacga tacgatatga acgcctttac cgccgcccac aaaaccctgc ccatccccag
     3301 ctatgtgcgc gtaaccaata ccaaaaacgg caaaagcgtc atcgtccgcg tcaacgaccg
     3361 cggccccttc cacggcaacc gcatcatcga cgtatccaaa gccgccgcgc aaaaattggg
     3421 ctttgtcaac caagggacgg cacacgtcaa aatcgaacaa atcgtcccgg gccaatccgc
     3481 accggttgcc gaaaacaaag acatctttat cgacttgaaa tctttcggta cggaacacga
     3541 agcacaagcc tatctgaacc aagccgccca aaacttcgcc gtttcgtcat cgggtacgaa
     3601 cctctcggtt gaaaaacgcc gttacgaata cgtcgtcaaa atgggaccgt ttgcctcgca
     3661 ggaacgcgcc gccgaagccg aagctcaggc gcgcggtatg gttcgggcgg tattgactgc
     3721 cggttgatgt cccgccttta cgtctttcaa acaccaccag atacttcgca ggagcaaaac
     3781 atgaacttca aacgccttct cttgaccgcc gccgcaaccg cactgatggg catttccgcc
     3841 cccgcactcg cccaccacga cggacacggc gatgacgacc acggacacgc cgcacaccaa
     3901 cacagcaaac aagacaaaat catcagccgc gcccaagccg aaaaagcagc gttggcgcgt
     3961 gtcggcggca aaatcaccga catcgatctc gaacacgaca acggccgtcc gcactatgat
     4021 gtcgaaatcg tcaaaaacgg acaggaatac aaagtcgttg tcgatgcccg taccggccgc
     4081 gtgatttcct cccgccgcga cgactgaatt tgatacaatc cgtgccgtct gaagcccgaa
     4141 ccggtttcag acggcatttt gcacccgaca cttcaggatt cggcatacat gatcagcaga
     4201 ctgaccggca aactggttga aaaaaaccct ccgcaaatcg tcatcgatgt caacggtgtc
     4261 ggctatgagg ccgacgtatc gatgcagacc ttctacaacc tgccgcccgt gggtgaaagc
     4321 gtacaactgt ttacccagct tatcattcgg gaagacgcac atcttttatt tggttttgcc
     4381 actgcggaag aacgcaagac cttccgccaa ctgatcaagg tcggcggcat cggcgcgaaa
     4441 acggctttgg gcattttgtc ggcgatgacg gcagacgagc tggcgcaggc ggttgcagaa
     4501 gaagatgtca aacgcctctc ctccgccccg ggaatcggca aaaaaaccgc cgaacgcatg
     4561 gttttggaac tgcgcggcaa gctggtcgcg catgcggtaa cggacgggct gtttgccgcc
     4621 gcacccgccg ccgacgaaac ggaagacatc gtcggcacgc tgcttgcgct gggttacagc
     4681 gaacgcgaag caaaagcggc ggtcaaaggc gttccggagg ggacggacgt gggcgaaggc
     4741 gtgcgccttg ctttgaaaaa cctgctgaaa taatgccgcc tgaaggcggc gcggcgtttg
     4801 ccctgacgaa accctgcgtt tccgcctcgc gctgtcttcc cgacggcttt tcccgtaaaa
     4861 tggcgttatt gtccttcctt tcagacggca ttgtgtttga tgccgtctga aactgtttta
     4921 ccgaaatcga aaacaatgtt gaacaaaata ttttcctggt tcgagtcccg aatcgaccct
     4981 tatcccgaag ccgccccgaa aacgccagaa aaaggcttgt ggcggtttgt ctggagcagc
     5041 atggccggcg tgcggaaatg gatagccgcc ctggctgcgc tgaccgccgg catcggcatt
     5101 atggaagccc tggtttttca atttatgggc aaaatcgtgg agtggctcgg caaatacgcg
     5161 cccgccgaac tgtttgccga aaaaggttgg gaactggcgg caatggcggc gatgatggtg
     5221 ttttcggtcg tgtgggcgta tgccgcgtcc aacgtgcgcc tgcaaaccct tcagggcgtg
     5281 ttccctatgc gcctgcgctg gaacttccac cgcctgatgc tgaaccaaag cctcggtttt
     5341 tatcaggacg aatttgccgg acgcgtgtcc gccaaagtca tgcagaccgc gctggcgttg
     5401 cgcgacgcgg tgatgacggt tgccgatatg gtcgtttatg tgtcggtgta tttcattacc
     5461 tccggcgtga ttctcgcctc gctcgactca tggctgctgc tgccctttat cggctggatt
     5521 gtcggtttcg cttcggtgat gcgcctgctg attcccaaat tggggcaaac cgccgcatgg
     5581 caggcggatg cccgctcgct gatgaccggc cgcattaccg atgcctattc caatatcgcc
     5641 accgtcaaac tcttctccca cggcgcgcgc gaagccgcct atgccaagca gtcgatggaa
     5701 gaatttatgg ttacggtgcg cgcccaaatg cggctggcga cgctgctgca ttcgtgcagc
     5761 ttcatcgtca acacctccct gaccctctcc accgccgcac tgggcatctg gctctggcac
     5821 aacgggcagg tcggcgtggg cgcggtcgcc accgccaccg cgatggcgtt gcgcgtcaac
     5881 gggctgtcgc aatacattat gtgggaatcc gcgcggctgt ttgaaaacat cggcaccgtc
     5941 ggcgacggca tggcaaccct gtccaaaccg cacaccatcc tcgacaagcc gaacgccctg
     6001 ccgctgaaag tgtcgcaagg cgaaatcaag ttcgaacaca tcgatttctc ctacgaagcg
     6061 ggcaaaccgc tgctcaacgg cttcaacctc accatccgac ccggcgaaaa ggtcggcttg
     6121 atcggacgca gcggcgcggg caaatccacc atcgtcaacc tgcttttgcg cttctacgaa
     6181 ccgcaaagcg gcacggtttt gattgatggg caggacataa gcggcgtcac ccaagaatct
     6241 ttacgcgccc aaatcggttt ggtcacgcaa gatacctcgc tgctgcaccg ttccgtgcgc
     6301 gacaacatta tttacggccg ccccgacgcg accgatgccg aaatggtttc tgccgccgaa
     6361 cgcgccgaag ccgccggctt catccccgac ctttccgatg ccaaagggcg gcgcggctac
     6421 gacgcacacg tcggcgaacg cggcgtgaaa ctctccggcg ggcaacgcca gcgcatcgcc
     6481 atcgcccgcg tgatgctcaa agacgcaccg attcttcttt tggacgaagc caccagcgcg
     6541 ctcgattccg aagtcgaagc cgccatccaa gaaagcctcg acaaaatgat ggacggcaaa
     6601 accgtcatcg ccatcgccca ccgcctctcc accatcgccg caatggacag gctcgtcgtc
     6661 ctcgacaaag gccgcatcat cgaagaaggc acacacgccg aactcctcga aaaacgcggg
     6721 ctttacgcca aactctgggc gcaccagagc ggcggcttcc tcaacgaaca cgtcgagtgg
     6781 cagcacgact gaaccgatgc cgtccgaaca cccgttttca gacggcattt ccacacccaa
     6841 ccccaaagaa accatgaacg acaccgccca aattaccgcc agctacggcc gccgctacat
     6901 tgtccgcacg cccgacggca caacctacga agccagcacg cgcaaaaaac gcgtcgattt
     6961 cgcctgcggc gaccgtgtcc gcatcagccc cgtcaacgcc gaacaagttg tgattgaaga
     7021 ttttttaccg cgccaaagcc tgctctaccg ccaagacgcg tggaaaacca aactcatcgc
     7081 cgccaacgtt acccaactcc tcatcgtaac cgccgccgtc ccgagtccga gcatgcggct
     7141 gctgcaacgc gccctgcttg ccgccgaagc cgccggcatc cgcgccgtca tcgtcctgaa
     7201 caaagccgac ctgcccgaaa ccgccctttg gcgcgaaaaa ctcaaattct acgaaacgct
     7261 gggttatccc gtcatcgaaa cccgcgcact ggaaaacgcc gacatcctgc gccccgtcct
     7321 gcaagggcac agcaacatcc tgctcgggca gagcggtatg ggcaaatcca ccctgaccaa
     7381 cgcccttttg ggcagccaaa ccgcccgcac cggcgacatt tccgccgcac tcgactcggg
     7441 aaaacacacc accacccacg cccggcttta tgatttgaac ggcgaaaccc aactcatcga
     7501 ctccccgggt ttgcaagaat ttggtttaca ccacctccaa gccgccgatt tgccgcgcta
     7561 tttccccgat ttccgccacc ttgtcgggca atgccgcttc cacaactgca cccaccgcgc
     7621 cgaacccggc tgcgccttca aagccgccgc cgaaaccagg gcggcaagcc ccgaacgcct
     7681 cgcctttttg cagggcataa ccgacgaact gcccgggtaa cgccttgccg tttgcaggac
     7741 aaaacaatgc cgtctgaagc cggattcggg tttcagacgg catccgtttt acaaaaatgc
     7801 tacaatccgc tttttactgg aacatccgaa gctatgaaga aaaacgcccc gaaatcgttt
     7861 gaagaggccc tgtcgcgcct tgaatcactg acccagtcca tgcaggggga aatgcccttg
     7921 gaagacgcgc ttgccgccta tcaggaaggc aacgagctgg tcaggtactg ccaaaccaag
     7981 ctggcacaag tcgaacagaa attacaggtt ttggatgcgg acgggccgaa ggagttgaac
     8041 cttgaatccg acgaatgatt tgaaagcgtg gcaacagagg gcgcaggcgc aaacagagct
     8101 gctgctggaa cgctttttgc cgtctgaaaa cgaaatcccg cacacgctgc acgaagcgat
     8161 gcgttatgcg gctttggacg gcggcaaacg tctgcgcccg atgctggtgc tggcggcttc
     8221 ggaattgggt gggactgtgg cggacgcggt cggacaggca atggcggcaa tcgaaatgat
     8281 ccacgtctat tctttggttc acgacgatat gccggcgatg gacaacgaca gcctgcggcg
     8341 cggcaaaccg acctgccaca tcaaatatgg cgaagccacc gcgctactgg tcggcgacgc
     8401 tttgcaaacc caagccttcg atgtattggc gcgtccgtcc ggcctgcctg cttcgaggca
     8461 gctggcaatg ttgtcagttt tggcaaaagc gggcggaagc aggggtatgg cgggcggtca
     8521 ggcaatcgat ttggcgaatg tcggcaaaca aatggttcaa gccgatttgg aacagatgca
     8581 cagcctgaaa acgggtgctt tgattcgtgc ggcggtttta ttgggggcga tgtcctgccc
     8641 gaatctgtct gaagacagtc tggcggtatt ggatgattat gcggcaaaat tgggcttggc
     8701 gttccaagtc atcgacgatg tgttggactg cgaagcggac acggcaaccc tggggaaaac
     8761 agcaggcaaa gacgcggaca acgataagcc gacttatgtg aaactgatgg gcttggaagc
     8821 ggcgcgctca tacgcacaca aactggttgc cgaagcggtc gcgctgctcg aacccttcgg
     8881 cgacaaagcc ctgcgcctgc ggcagttggc agaatttgca gtcgcccgca aatattaaaa
     8941 ccggcgtata tcgccggctg aaacgtgccg gcacagcgat gccggcctgc acttgaatga
     9001 aacaaatgcc gtctgaaagg gtttcagacg gcattcctat gcctgcctat tccctgttgc
     9061 gcgtgtgcca aaaataccgc cacacgaaac agggaaacgc aaaaatcagg tacaggcaga
     9121 ctacggtggc gtaaaactcc caaccctgat cgtgtaccgc tcccgcacgg gattcgagga
     9181 tgtaggcaag aacggcggtc agcgcgaaac ctgccgccag ctcgatcaag tggtgcccga
     9241 aatgtttgcg cttgagcggt acgatgccga acagcctggt cgtgaggaag ggggcgttgg
     9301 caaaaatcaa ggcaagcagc aaaaggatgt acatggatgc ggtcatggcg ggctttctgt
     9361 ttgggcattg tcggcggcga tgccgtctga actgcgttgc ggaacggcat atcttaacaa
     9421 aaacggcagc ctctgacacg gctgccgttg cggcggctta cagcgtgttt tccaacgcct
     9481 tggcgcacca gtcgataacg gtttgcggca taatgcccca cagcaccaac agcagcgcgt
     9541 tgacgctcaa aaggaatttt gcggcattgt tgccgccgac ggtgggacgg gcgcggccgg
     9601 attcgtcaaa atacatgact ttgaccacgc gcaggtagta gaacgcgcca atcagcgaca
     9661 tcacgacggc aaatacggac aaccaaacat agccttgttt taagagtgcc ataatcacgc
     9721 cgaatttggc gtaaaagccc atcagcggcg gaatgcccgc catagagaac ataaccagca
     9781 gcatcaaaaa ggcaagccat acgcggtgtt ggttcaaccc tgccaaatcg ctgatgtttt
     9841 cgcactcgtt gtccccatcc gacaacacca tcaacacgcc gaaccctgcc gccgccatca
     9901 gcgcgtaggt aatggcgtaa tagagacccg ccgcaaagcc gaccgcgcct gccataaacg
     9961 ccaacaagat aaagcccata tgcgatacgg tggaataggc gagcatacgt ttgatattgg
    10021 tctgcatgat ggcggcaagg ttgccgacca gcagcgaggc ggcggcaagc agggcaaaca
    10081 tcagagacca gtcatgatgc acggttccca gcccggtaac gaggatgcgg aaagcgaaaa
    10141 cgacggcggc gactttcggg gcagtgccga ccaaggcggt aacagaagtg ggcgcgccgt
    10201 gatacacgtc gggcacccac atatggaacg gcaccgcgcc gagtttgaac gcaacggcga
    10261 cgacgataaa caccagaccc agtttcaaca gccattcatt ggcttcttca ttgaaggaag
    10321 aggcgagcac gccggcaaat tccagcgaac cggttgcgcc gtaaaccata gaaataccgt
    10381 agagcagcag gccggatgcc agcgcgccca aaacaaaata tttcaaggcg gcttcggcgg
    10441 caaagccgga atcgcggcgc agggcaatca gggcgtaaag ggcaagcgac aagagttcca
    10501 aaccgatata ggcagttaaa aaatgccccg cgctcaccat cacgctcatg cccaagaggg
    10561 caaacagcga cagggtgtaa aactcgcctt tgaaaatacc gcgcacttgg ttgtagggct
    10621 tggcataaac aaacagggca aacgtcaagg catataaaac catttttgcc aaacgcgaca
    10681 taccgtctgc aatatacatg ccgttgaacg aagacgtgct gccctgttcc caaacggcaa
    10741 gctgcaccac agccgtaacc gccaccgttg ccaacgcgcc gtaatgcgtc caacggcgtt
    10801 tgtcatcact gacccacaag tctgccagca acaataacac cagcagcgac agcagcgcga
    10861 cttcgggcat ggcgggcatt aaattcaaat cagaccagtt catttacaca cctcaaatct
    10921 tgctttgtgc cacatgggca atcaaatcgt ttgccgcctg atgcaccact tcgataaacg
    10981 cttgcggata cagacccata cccaatacca caaccgccaa aatcgccaga atcgcaaatt
    11041 cgcggcagtt gatgtctttc atttcggcaa cgtgcggatt gtggatcgcg ccgtaaatca
    11101 cgcgcttgta catccacagg gtataagacg cgccgtaaat cagcgtcata gccgccaacg
    11161 cgcccaccca gaaattcact ttaaccgcgc ccataatcac cataaactcg cccacgaagc
    11221 cggaagtcgc cggcaaaccg gcgttcgcca taccgaacaa catcataaac gccgcaaact
    11281 tgggcatcac gttgaccacg ccgccgtaat cggcgatatt gcgcgtgtgc aggcggtcgt
    11341 acatcacacc gatgcacata aacatcgccg ccgacacgaa accgtgcgaa atcatctgaa
    11401 tgaccgcgcc tttcaatgcc cagtcgtcca actgcccgtc aacaaacaaa aacatcccaa
    11461 gcgttacaaa acccatatgg ctgatggacg aatacgccac cagttttttc atatcggttt
    11521 gcaccaaagc caccataccg atataaatca cggcaatcag actcaatacg atgatcacgg
    11581 gggcaaaata gcgtgccgca tccggcataa tcggcaggat aaagcgcaag aaaccatacg
    11641 cacccagttt cagcgtaatc gcagccaaca ccatcgaacc gccggtcggt gcttcaacgt
    11701 gggcatccgg cagccaagtg tgtacgggga acatcggcac ttttacggca aacgacagga
    11761 agaacgccgc aaacaaaagc tgttgtacgc ccaacggaat ctgtttgatg ttttggaaat
    11821 cgacaataga gaagctgcct gtttgatagt acaggtaaac catagcaacc agcatcagga
    11881 gcgaacccat cagcgtatag aggaacagct tgaccgacgc atagacgcgg cgcgggccgc
    11941 cccatacacc gataatcagg tacagcggaa tcagcatacc ctcgaagaac acataaaaca
    12001 gaatcgcatc ctgcgcggca aacgcaccgt taatcaaacc cgacatcatc aggaatgccg
    12061 ccatatactg cgccggacgt ttctgaatga cttcccaacc agccaatacc accaacagcg
    12121 taataaacgc attcaagatg ataaagagca ctgaaatacc gtccacgccc aatgcgtagt
    12181 tgattttcag cagcggaatc cactcgtgga actcggtaaa ttgatagccg ccgctcaaac
    12241 ggtcgaaacc ggtaaacagg ggcagcgtta ccaagaaacc ggcaagcgca cccatgaagg
    12301 cgagcacacg cgccagcggc gcacggctgt ctttaccggt cgccaaaacc agcacacctg
    12361 cggcgatggg tatccatatt gccaagctga gtaggtagtt ggaaaacata gtggttaacc
    12421 tgtggttaaa ataaaaaata tagttgtatg ggttggtctt tacagacagc gttttaggaa
    12481 gtcgtctgaa agaagggata aatacactcc tccttcttta cttactccca atcaatatct
    12541 gtacgattgc gatattctgc ctgtactaaa acatctaatt cagccaaatc ttttatttct
    12601 gactgcatag ctagtaaagc tcttaacttc atacttaaat caatatattt ggagccttcg
    12661 cttgctcctt ttgcagactt aaatttattt tcttctaaaa attttttaat gggagcattt
    12721 aaaacaggat atctatctgg atattcttga caaagcattt cagaaaaaac tgaaccacga
    12781 gtggaaaccc cacattcttt tagccaatca atttgcttgg caaccacatt atctctctca
    12841 gtaagtggag catcaaaaac tgattggatg gcaatgcata attttctaaa atctgaagac
    12901 ttacccgtcc ttttccacgt tttatcttga aaacgattgc ctaccccttc tcctttattt
    12961 tcgcttttcc aagaccataa tttattaaat tccccataaa aatcattatt gtctattttt
    13021 cctgctgcac attgcttaat aagatttttt aattgattac acacagtttg atggtttctc
    13081 atctgctttc tccgtgccgc aatcaattct tgattataat ttggcatctc cataaataac
    13141 ttatccatca ctgattgtct aacagttgat ttcttatagt taatttcagc ttcctgatac
    13201 tcctcaagcc aatcttccga taccgggata gatttcatca caatttcatt agcccaagat
    13261 ttagctttta taaaatcctg ctcagaaatt ttagtcaaaa tatttgcttc ataattgcta
    13321 ttaaacgcag catgagtcaa atttgaagaa ccgattaaag cataatattc attcttatca
    13381 ttcttccaaa atacagcttt aggatgaaaa ccctgaatgc cttgagctac catcaatgta
    13441 aatttcaaat gggacggaag ccatttcaat acttcctgaa tagcctcttt tttagacaat
    13501 ccgaaatcct gcccaaaaat aaatttgaac tcttgtaatt tgctattaag cttatttttt
    13561 acatcccaat gagtgagata cgcagagaaa atatacagct ctactgcctc tgaaacagct
    13621 ttctcatata ttgaaatcag tttcttatca tttggtgtat ctgaatgtaa aaataactgc
    13681 gtcatctctt cttaccccaa cctacaattc cattgcagcc tgaaaccctc ttttcagacg
    13741 acctcaagca ctgccttctt tctcaatcac caaaatccgc gcttcgccgc acggatgggc
    13801 gacgtgttcc gtgccaattc ctgcgtagaa gatgtcgccg cttttcaacc gaacgatgtg
    13861 ttcttcgccg ttttctcgat agtgcatatc gacttcgccg tccatcacgg caaacacttc
    13921 ctcgccgtcg ttgacgtgcc atttgtagga ctggtccgtc cagtgcaggc gcacggtggt
    13981 gccgttcata ttggcgatgt cgagtgcacc ccatgcgcgg gaagcggtga agtcggcaga
    14041 gcggatggtt tgtgtgtttg acattgtttc tcaagtttct tttttcagac gacctctaca
    14101 caaccatgaa caggtcgtct gaaaccggcc tttatctgaa caagccccag aaggtcatac
    14161 cgagcagtac caatacgccg aataccatag cggcggcgta ggtgtagata aagccggttt
    14221 gggctttacg cacttgagcg gcaaccgcgc cgaccagttt ggcggagcca ttgacgatgc
    14281 cgttgtcgat aatggcggta tcgccgactt tccaaaagaa ggtacccaat gcgcgcgtgc
    14341 ctttggcgaa aacgttgaaa tacagggcat cgaggtagta tttgttttca aacaaaacgt
    14401 aaaccggacg gaacgcctgc gcgattttgg cgggcaggtg cggcagtttg acgtacaaaa
    14461 gccatgcgct caacacgcct gcgatagcaa ggtagagtac gggcgaatgc aggctgtgcg
    14521 acaccattgc caatgcgccg tggaactctt ccttcatgat gtgcatggtc ggatgcgcgt
    14581 cggtattgac gaaaatcacg tctttaaaga aatcgccgta aagcataggc tcgatggcaa
    14641 cgtagccgat gatgacggac ggaatgacaa gcaaaatcaa aggcagggta acaaccaacg
    14701 ggctttcgtg cggattgtcg tttttgccca agctgtgatg ttcttcgcca tgatgatcgt
    14761 catgatgttc aggcaagctg cgccatttct cttcgccgtg gaacaccata aagtattggc
    14821 ggaatgcgta gaacgcagta acaaacacgc tggcgaggac ggcgaaatag gcaaagccgc
    14881 tgcccggcag ggtgctgtat ttcgccgctt cgataatcga atctttggag tagaagccgg
    14941 agaagaacgg cgtaccgatc agcgacaaat tgccgatcag catagtcagc caagtaatcg
    15001 gcatgtattt tttcaggtta cccatgtggc gcatatcttg gtcgtggtgc ataccgataa
    15061 tcgcgctgcc cgccgccaag aacagcaagg ctttaaagaa agcgtgggtc atcacatgga
    15121 acatcgccac ggaataggca gacgcgccca gagccacggt catatagccc agttgcgaca
    15181 gggtggaata cgcaaccaca cgtttgatgt cgttttgaat cacgcccaag aaacccataa
    15241 acagggcggt aatcgcacca atcaccatga tgacagacag cgcagtgcta ctcatctcat
    15301 aaatcggcga catacgcgat accatgaaca gaccggcggt aaccatcgtc gcggcgtgaa
    15361 tcaatgcgga aatcggggtc gggccttcca tcgaatcggg cagccagaca tgcagcggga
    15421 attgtgccga tttacccatc gcaccgacaa acagaagtaa acaagttacg ataatcaaag
    15481 accattccac accggggaaa agctggatgg tggcattttg gacgttgggc agataagcga
    15541 atacatcttg atagcgcagg ctgccgccga aataggcaag caccaagccg ataccgagca
    15601 aaaagccgaa gtcaccgaca cggttgatca aaaaggcttt caggttggca aatgttgcgc
    15661 tcgggcgttt gaaatagaaa ccgatcaaga gatacgatac caagcccaca gcctcccagc
    15721 cgaagaagag ctggatgaag ttgttgctca taatcagcat caacatactg aaagtaaaca
    15781 aagaaatata gctgaagaag cgttggtagc cgactttttc atcgtgcata tagccgatgg
    15841 tgtagatatg caccatcaac gacacgcccg ttaccacgac catcatcatc gccgtcatcg
    15901 tatcgaccaa gaagccgacg gagaaatcca aaccgcccat tgtcagccag gtatagacgt
    15961 tctcgtcaaa cttggcacgg ctgccattga ggaatcccca cagcacataa gccgacagca
    16021 cggcggacac cgccacaccg agtatcgtaa ccgtatgcgc gccggcacgt ccgattttgt
    16081 tgccgaataa acccgcaatc agcgagcctg ccaaaggcac gagggcgatg atgagatata
    16141 aagtcatgtc gttcatttga ttgaatccga ttgatttaaa aatctatgtt tgtttcgtac
    16201 aaaattactt cggagaaaca aatccaacac gctccaatcg tttgcgtgcc acagctaatt
    16261 gctcttcagt aaataaatca caccacggct tttgtaacac cagatattcc atactgtatt
    16321 tcaaggcgtg gactccgcca ccactcaaaa tcagctctgt aaaaccggtc tgagtcttct
    16381 tttcccccgt acttaataat ttatccgccg cctctttacc accaaattca tttacaattt
    16441 gtaaaaatcg tgtcgccttg taaggttgcg gcaaattcaa agcctcctga taaatattta
    16501 acatggcttt atgaaattct tgttctaact gatttttatc catcattctt cttccaatat
    16561 ttcagaccgg attattctta cccagaattt cttttctcat ccgctcccgt ctgatcacct
    16621 accaaatcag gtcgtctgaa acagtctgaa atcgcttttc agacgacctt cagccttttt
    16681 catacccttc gtaataatac gactgctcga tacctttaaa gatgatttca cggttgtcca
    16741 catcgtcggt caggttgtcc tttaacaaaa agcgcagttc taaatcgttg acggggctgc
    16801 gttccatcgc ctgcaaatac agggttttgc ttacattttg ccagttcacg acttttttca
    16861 ggtttttctt cagcaccaaa tccagccaga tgcgggtact tctgccatta ccctccaaaa
    16921 acggatgggc aatgttcatt tcaacatatt tggcgatgat ttcttcaaaa gtccgctcgg
    16981 gcatctgctc gattttaacc aaagcctctt ttaaatacat ggcgttggca aaacgaaaac
    17041 cgcctttgga aatgttgtct tccctgattt gacccgcaaa atcatataag ccgccgaaca
    17101 ggtaacggtg aatctgttgc aggcccgcgg tggtaccgac ttcgatacgg tcgatgtcgc
    17161 cgctttcaaa caggcggcgg gcattatgca ggctttgttc gtctatggat ttcatcgttt
    17221 ttcctatcgg gttttcagac ggcatcggtg tcttgtcagg tctcgaccca acctaccttt
    17281 accctttcaa ctcgtccaaa tcggcaacgt taatcgtttg tcggttgcgg tacaccagca
    17341 ccataatcgc caaaccgatg gcagattcgg cagcagcaac ggtcagtacg aaaaatacga
    17401 aaatttgtcc ggcagtatcg cccaaatgtt gcgaaaaggc gataaagttg aagttcactg
    17461 ccaaaagcat cagctcgatg gacatcagca agaccagcac gtttttgcgg ttcataaaga
    17521 tacccatcgc gctgataccg aacaggagcg cacccaatac cagataatgc gtcaaggtaa
    17581 tcatgctttg ccctcctctt tcggcttgag gtcgtctgaa acttcgcttt cttcggcaga
    17641 ttcggtttgc ggtttcacgg cttccatttt caccaagcgc atacggcctt ggtcggcgcg
    17701 tactttaact tggtcggcag gatccatacg tttcggatta accgttttac ggtgaaccag
    17761 cgcaatcgcg gccaccatac ccaacagcag caataccgcc gccaattcaa acggcagcag
    17821 ataatcggta taaatacggc tgcccaaatc gcggatattg ttgtaatcgg cgggaatgtc
    17881 tttcatcagc ccaaatgcgg caaggtcggt tttcggattg accagaatca gaatcagggc
    17941 aactgccaac aatgtgccga ccacaccggc aacaggcgcg tgccgccaga aaccggcacg
    18001 catttcttca atgtcgatgt tcaacatcat cacgacgaac aggaacaata ccatcactgc
    18061 gccgacatac acgaccacca gcgtaacgcc caaaaactcc gcctgcatca gcatccaaat
    18121 catcgcgctc acgcagaagg tcaacaccag atgcaaagcg gcgtgaacag gatttttagc
    18181 ggtgacggtt ttgatcgcgc cataaagaat tatcactgca aaaatataaa ataaaatcag
    18241 ttggaaagtc atagtctatg ctttctttat taatcaaaaa tatggtttca agcagtctga
    18301 aaatcctggt tacgcaaaca atcaaactta atcagttgat ccatatccgc acaaatccac
    18361 gttgtcgcaa ttttgaataa caacatttct atgtgatacg gttttgcttg tttaccataa
    18421 acttgtttcg ccaaatcttg taacaatcca caatattgat gaaacgaaac gtctggtttc
    18481 cgataattcg gtttgatatc agaaaaatga aacaatacgt tcatatttcg gatttctagt
    18541 tccctatttc taccttgtgg ctgaaaaaac aaatctgcat ttgtaaattt aaacaacaac
    18601 caattcaacg aaaaaatagc gcgtgaatca taaatggcga atcgctcaca atctataaaa
    18661 gaagccaatt tagataatga tgaaattttc aacaaaccac tactcaattt tcccgaatta
    18721 agttccgata aaaattgacg aataagctgg ctattttcag cagattgttt aaaactttta
    18781 atcccacccc attcacggat aatccaataa tgcgtcttca agctatcatc attttgtaat
    18841 tttggggata aattttcttt taaaaaaata ttttgttcaa aattacttaa tccacttggc
    18901 aactcaatcg gactatttct agttctaatt tcttcaaaat tatctattgg ctcatgttcg
    18961 gctaaatatt tcaatgcatt aatcagtcct tccatattta aaaccttcca agaagcccca
    19021 aagcattaac gatacggcgc gtcagcggct ttgcgtttgg ctatttcagc ttcgtattta
    19081 tcgccaatgg ccaaaagaat cggcttggtc atgtgcaagt cgcctttttt ctcgccgtgg
    19141 tattcaaaaa tatgggtttc cacaatcgca tcggtcgggc aggcctcttc gcagaaaccg
    19201 cagaagatgc acttggtcag atcgatgtcg taacgcttgg tgcggcgggt accgtcttca
    19261 cgttcttccg attcgatgtt aatcgccatt gccggacaaa ctgcctcaca caatttacac
    19321 gcaatacagc gctcttcgcc gttcggataa cggcgttgcg cgtgcaggcc gcggaaacgc
    19381 acggattgcg gcgttttctc ttcgggaaaa taaatcgtgt ctttgcgggc gaaaaagttt
    19441 ttgagcgtta cgcccatacc ttttaccaat tcgccaagca gaaaggtttt tactaagtta
    19501 gccatattat gttccctcaa aacagggatt tcgttaggta ttcaaaatcg ctttgttcag
    19561 acggcctcaa gatgccgtct gaaacttatt tccacaaatt cagcggtgaa atcatccaca
    19621 cgcccaaaac cacgatgtag gcgaagccga tcggaatcag cactttccag cccaaacgca
    19681 tgatttggtc gtaacggtag cgcgggaagg ttgcacggat ccacaggtac cagtacaaca
    19741 ccgccgccat tttcacgaac atccagaatg cggaaggtgt gccgataaag ccccaacttt
    19801 gcgggaacgg agacagccag ccgccgagga acatcagcga tgtcagcgcg gcaatcagaa
    19861 tcatgaaaat gtattcggca aggaagaaca gcgcgaatgc gaagccggag tattcgacgt
    19921 ggtgtccggc aacgatttca gactcaccct ctgccacgtc aaacggcgcg cggttggttt
    19981 cggcaacggc ggaaatcaga tagacgataa agatggggaa gagcggcagc cagttccaag
    20041 agaataccga accgccggca atacctttgg cttgtgcggc aacgatgtcg gagaagttca
    20101 tgctgcccga taccatcacg acacacacca gcgcggcact catggcaatc tcgtaggaaa
    20161 tgctttgtgc ggaagcacgc attgcgccca aaaacgaata tttggagttg gaagcccagc
    20221 ccgcgatgat cacgccgtaa accgacagcg aggtaatcat caggatgtac aaaagaccga
    20281 tattgatgtt ggtcagcacc cattcttcgt taaacggaat caccgcccac gccgcaaaag
    20341 acggggcgag cgacataatc ggaccaatgt agaacagggc tttgtttgac agcctcggac
    20401 gggttacttc tttaaacaag agtttgaaca cgtcggcaaa cggctgaatc agaccccacg
    20461 gaccggttac gttcggaccg acgcgaagct gcatgaagcc gatgacttta cgttcaaaat
    20521 aagtcaggta ggcgacggtc agaatcagcg gaatcaggat aatcacaatt ttgacgatga
    20581 cggataccac caagcctaca gtaataccca aatcgcccag accgagcgtt gcggcaaaga
    20641 ggttttggaa ccattcttgc atgatcaagc tcccgccagt tcaatagtgt ccatcaacgc
    20701 acccagcgcg gcattttcgg tatgcagcgg caaatgcacc acgttttcag gcaaaccggc
    20761 atcggctttg acggcaaccg atacgcttgc gccgttttgt ttggcgacag cggtttgtcc
    20821 gtcttgcagg cccaagcgtg ccaaagtgtt cggattgaca cgcgcggcgg gcacggcggc
    20881 atggctggtt tcttgcaacg gtgcggaacg gcgcacgata gcatcggtgt gatagatgcc
    20941 cactccgccc acgcgcacga ggcggcttga tgccgtctga aaatcttttt gagtcgaagc
    21001 ggcgcggttg ttcagtttgg acggcaggct ttccgcatcc agcgcgtctt tcagaatcgc
    21061 gacggtatcg tggtattcaa aacctttcag gtcaaacagg ttgcccaata cgcgcaacac
    21121 tttccacatc ggacgcgaat cgccgaagcc ttgtaccacg ccgtggaaag attgcagacg
    21181 gccttccata ttgatgaagc tgcccgaggt ttcggtaaac ggcgcaatcg gcaacaatac
    21241 gtcgcacacg tccagcagcg tttcgctgac aaacggcgta aacgccatca cgcttttcgc
    21301 ttgtttcaac gcagccacgg cttttgcacc gtcaaccgta tcgatttcag gctcgacgtt
    21361 gagcagcaag actgcctgtt tcggcgcgtt tgccatttcg gcaacgctct cgcccgagtt
    21421 cacgcccaag acatccgcac caacgctgtt ggcggcttgc ggcaaaatac ccagcactgc
    21481 gccggtcgca tctgccagct cttgaacggc ggcataaatc gcggcgtaat caggatggtt
    21541 ttgcacttcc gcgcccaaaa tcaccgctgc tttttcagcg tttttcaggc tggcggtaac
    21601 ggcgtgttcc gcatcggcag acaggttttt cagacgacct gcccactcgt cgggatgtac
    21661 ggcttcttga gacagaagcg gcataaacaa ttcttcttta ctgcttgcca atacgctcaa
    21721 agccatgcgg tctttagcgg cgcggcgcag gcgggcggtc agaagcggct gttctttgcg
    21781 caggttcgca ccgactacca atacggcatc gttgtccgcc agcgattcaa tgctttgtcc
    21841 caaccattgc gcacctttaa tgccgtctga aagacgtttg tcttgttggc gcagacgggt
    21901 ggcaaagttt ttaacaccca agccgtcggc aagctttttg gccaaataca gctcttcaac
    21961 cgtattcatc ggattggccc aaacgccgac ttgattttgg ttgccgtctt tggcgataca
    22021 tttaattgcg ctgtggacat attccaacgc ggttttccaa tccacgtcca tccactcgcc
    22081 gccctgtttg attttcgggt ttttcaaacg gctttcgtga tacaggcctt cataggcaaa
    22141 acggtcgcgg tcagacagcc aacattcgtt aatcgcttcg ttttccagcg gcaacacgcg
    22201 gcggacggta tggtctttgg tttgtataat caggttgctg cccaaagcgt catgggcgga
    22261 aacggatttg cggcggttca attcccaagt acgcgcgttg aagcggaacg gtttgctggt
    22321 caatgcgccg acaggacaca aatcgatgac gttgcccgac agctcggttt ccaccgtttt
    22381 gccgataaag ggcatgattt cggagtgttc gccgcgattc accatcgcaa tttcctgcaa
    22441 accggcgatt tcttcagtga aacgaacgca gcgggtgcag tggatacagc ggctcatttc
    22501 ctcggcggaa accaaaggcc ccatgtcttt gccgacgacg gaacgttttt cttcggtgta
    22561 gcggctggtg gtttttccgt agcccaccgc caaatcctgc aactggcatt ccccgccttg
    22621 gtcgcaggtc ggacaatcaa gcggatggtt gatgagcagg aactccatca cgccttcctg
    22681 cgcctctcgg gcttttgccg aatgcgtacg cacaatcatg ccgtctgtaa ccggcgtggc
    22741 acaggcaggc aggggttttg gggctttttc tacgttcacc agacacatac ggcagttggc
    22801 ggcgatggaa agttttttgt ggtaacagaa atgcggaata taagtaccga gcttgtgcgc
    22861 ggcttcaatc accgtcgcgc cctgctccac agatacttgt ttgccgtcga tttcgatttg
    22921 taacatggtt cgttcctagt tacgggtatt tgataaataa tcttttatgg aggtcgtctg
    22981 aaaaatgggt ttcagacggc cttttgagtt tcattgacaa tcaatactgt ttaaaacgtt
    23041 tactgccacc ttgccgtcat tcccgcacag gcgggaatcc attttttgaa tttcggcaac
    23101 tgcttttcaa atatcgggtt ctgtaaattc cactatggat tcccgcctac gcgggaatga
    23161 cggcaaagtt aaatttttag cattttgctt ttaaccaata taaaaccaac taaaactata
    23221 acttatttat tatattttta aatgacttaa ataactcttt aacagcacgc tcattgtctt
    23281 catctgtagg ctcaaacttt tcacaccaat taattatatt tacatcgcca tgcaataact
    23341 ctgcataaca tcctactgca tcgcaactac caccccgtgt ccttgacgtg ccaaaaataa
    23401 aatcacaatt atgcgaataa agtttgtata aatttctcct aacctcgtat tcattatcac
    23461 cagatgaata aaaaccaatc ctctgacctt catgatcaaa tactgcaaca aaatctaagc
    23521 cgtttccatg tctttcaaaa aagactagaa atttagaaaa tttccgacaa atctgattaa
    23581 aaagcgtatt gagtgtcgta ctcttacctt tgttggctgc accgtataga ataaaaattt
    23641 tagcttccac cattaccacc ctccccactt atgctctttc atcggcctgc cgtgttcgat
    23701 gtaatgcaca aactcatcac ggaaatgctt ggtaaagctg cggacaggga agacggcggc
    23761 atcggcaagg gcgcagatgg tgcggcctgc catttgtttg ccgacagaat ccaacaaatc
    23821 caagtcttcc atacgacctt tgccttctac gatgcggtgg acgatgcggt aaagccagcc
    23881 ggtaccttcg cggcaggggg tacattggcc gcaagactcg tcgtagtaga agtagctcaa
    23941 acgctcaagg gctttgacca tgcatacgtc ttcgtccatc acgataatcg cgccggaacc
    24001 gagcatcgag cctgctttgg aaatcgaatc gtaatccata ttggtctgca tcatgatatc
    24061 ggcaggcaat acaggtgcgg acgaaccgcc gggaataacg gctttaagtt ttttaccgcc
    24121 acgcataccg cccgccattt tcaagacttc agcaaacggc gtacccaatg gcacttcata
    24181 gttgcccgga cgctcgacat ggccggaaat acagaacaat ttggtaccgc ctgcattcgg
    24241 aataccttta tcggcaaatg cctgtccgcc gtcacggata atgaatggaa cggaggagaa
    24301 cgtttcagta ttgttgatgg tagtcggttt gccgtacagg ccgaacgaag cagggaatgg
    24361 cggcttaaag cgcggctggc cttttttgcc ttccagcgat tcgagcaatg cggtttcctc
    24421 gccgcaaata tatgcgccgt agccgtggtg ggcgaagagt tcaaattcaa aatccgaacc
    24481 caaaatattt ttacccaaaa agcctgcggc acgcgcctgc tccaaagcgg cctcaaagcg
    24541 ttggtagcct tcaaaaattt cgccgtggat atagttgtaa ccggctttcg cgcccatcgc
    24601 gtaaccggcg ataatcatgc cttcgatcag ggcatgggga ttgaacatga tgatgtcgcg
    24661 gtctttaaat gtacccggct cgccttcgtc ggtattgcaa acgacgtatt tttcgcccgg
    24721 aaaggaacgg ggcataaagc tccatttcaa accggtcggg aagcccgcac cgccgcgccc
    24781 gcgcaaaccg gaggttttga cttcgtcaat cacatcggtt tgcgagatgt tttcggacag
    24841 aattttacgc agggcggtat agccgccgcg tttgacgtat tcgtccaatg tccagcaatc
    24901 gggattggcg gtatccactt ggtcaaaaat cacgcctgat tggtaaatag ccatttttgg
    24961 tgtgcctgtt cgttttcgta tcggttgcgg ctgcctgccg tctcaatgtt cctgatgccg
    25021 tctgaaacgg tttgtctgtt ttcagacggc attttcctgt cttcgtttaa tctgaaccaa
    25081 acctgcgttt gttcttcccg tcatcaatcg aaattaccat ccgccgcaca tattttcaca
    25141 cggaatgccg tcgccatcgc cgtccatctt ggtatcggaa cagttccttg caaaccattt
    25201 ggcttcttcg cacgaagtca tttgtgagca atgttgcctg ccgtcgcaac ggtatgggct
    25261 gctgtcgggc tgctgccgtt ttatttcggg taatacttgc gttttaaccg gcgtattttc
    25321 ctgtttcgga gtcagcttct ctgacgcata ttgatacgcc ttatagccga ttaccgaaac
    25381 aagtagtaaa ccgaccgaca aagacagcac aacctgaaca atcgaagtgg gagattcctc
    25441 gtcttttcga tgtttcttgc agtatccggg agaaactgca cgctcccgat tcagatagcg
    25501 tatctgtttt gcagaaaatc ttcccttacc atctgaaacc acttcaaaac caaccgcctc
    25561 gttaatctca ggacgcgggt ttcttgggga aaagtcagaa atatggcaga aaatttcttt
    25621 gttaccgcca tctatccgta taaaaccata acctttttcg tcatcccact tgacgattac
    25681 ccctctcatt aagcactccc cttttttatt gttgcttttt aaaatcattc agtaggtcgg
    25741 attcttgaat ccgacatttt gcctctttac ccactctgtc ggatacaagt atccgaccta
    25801 cgtttaaatc gtcgtttcag acgacctact ccaattccgc cagtttcttc tcaatcgcct
    25861 cttcggtcat aaagccgcac atactgtggt tattgaccag cataacaggg gcatcgccgc
    25921 acgcgcccat gcattcgcct tcgacaaggg taaatttgcc gtcgggcgtg gtttcaccgt
    25981 agccgatacc gagtttttgt ttgaggtatt cgccggtagc cataccgccg cgcaaggcgc
    26041 agggcaggtt ggtacaaacg gtcagtttgt atttgccgac cggctcgagg ttatacatat
    26101 tgtagaaagt agcgacttcg taggcttgtg caggcgagat gccgatatag tcggcaacaa
    26161 aggcgatggt ctcgggagca agccagcctt tttcggtttg ggcgatacgc aacgcgccca
    26221 taatggcgga acggcgttgg tcggcgggat attttgccaa ctcgatgtcg atttgtttta
    26281 aggattctgc ggataacatt atcggtcaac ctccccaaat acgatgtcct gcgtaccgat
    26341 gatggcaacg acgtcggcga gcatatggcc ttttgccatt tcgtccatgc cttgcaggtg
    26401 ggcgaagccg ggtgcgcgga ttttcaggcg gtagggtttg tttgcgccgt ctgaaatgat
    26461 gtaaacgccg aactcgcctt tcgggtgttc gacagcggtg taggtctcgc cctcgggaac
    26521 gtgcataccc tcggtaaaga gtttgaaatg gtgaatcagg tcttccatac ctgttttcat
    26581 ttcggtacgt ttgggcggag caaatttgtg gtttgtggtg atgaccgggc cgggattgac
    26641 gcgaagccag tcaacgcatt gtttgatgat gcgtacggat tggcgcattt cttccatgcg
    26701 gcagaggtag cggtcgtagc agtcgccgtt cacaccgaca gggatgtcga aatccatttt
    26761 gtcgtacact tcatacggct gtttcttacg cacgtcccat tccacgccgg aaccgcgcag
    26821 catcacgccg gtaaagcctt tttgcatggc gcgctcgggg gagacaacgc cgatgccgac
    26881 ggtacgctgt ttccaaatac ggttgtcagt caggagggtt tcgagtgtat cgatgttttt
    26941 cgggaaacgt tcgcagaagg cgtcgataaa gtcgagcatg gtgccttcgc gggattcgtt
    27001 gagctgcttc aatactttgg cattgcggaa tttgctgccc tcgtatttgg gcataaagtc
    27061 gggcaggtcg cggtaaacgc cgccgggacg gaagtaggcg gcgtgcatac gcgcgccgga
    27121 aacggcttcg tacaagtcca tcagctcttc gcgatcgcgg aaggcgtaaa ggatggcggt
    27181 catcgcgccg atgtcgaagg catgcgaacc gatgcccatc aagtgattga ggatgcgcgt
    27241 tacttcggcg aacatcacgc ggatgtattg ggcgcggata ggtacatcaa ttccggcgag
    27301 tttttctacc gccaagcagt atgcctgctc gttgaccatc atggaaacgt agtccagcct
    27361 gtccatataa ggcagggctt gcagataggt tttggtttca gccagttttt cagtaccgcg
    27421 gtgcaagagg ccgatatgcg ggtcggcacg gacgatttgt tcgccgtcca actccaaaat
    27481 catacgcaat acgccgtgcg ccgcagggtg ttgcggaccg aagttgatgg tgtagtttct
    27541 taatttattg gccaccgtag ttctcctcac ggacgatacg aggcgtgatc tcgcgcggct
    27601 caatggtaac aggttggtaa atcacgcgtt tttgctcttc gtcgtaacgc atttccacat
    27661 agccggaaat cgggaagtct ttgcggaacg gatgtccgac gaaaccgtaa tcggtcagga
    27721 tgcggcgcaa gtcagggtgg ttgttgaaca tgatgccgta catatcgaag gcttcgcgtt
    27781 cgtaccaatc cgcgctgttg taaatatcga ctacggattc gactacgggg aagtcgtcgt
    27841 ctgaaaccca gacgcgtacg cggatgcgtt gattgttttt aacggaaagc agctgactga
    27901 cgacggcaaa gcgtttgccc tgccatactt cgtttttgta agtgctgtaa tcgacaccgc
    27961 acaagtcaac caagagctcg aaatgcagct cttcatggtc gcgcagtgcg atcatgactg
    28021 aaatatagtg ctcgggcaga cactcgacgg taatctcgcc caaagcggaa atgactttgc
    28081 ttgcctgatt gccgagaacg cggctgacgg tttcgtataa gttttgaatg cttgccatat
    28141 cgtcctctcc ttactcgtca cgcgcgatgg tggaagtgcg cttgattttt tgttggagct
    28201 gaatcaggcc gtaaatcagg gcttccgcag tcggcggaca acccggcaca taaacatcta
    28261 ccggcacgac acggtcagca ccgcgcacaa cggaataaga atagtgataa tagccgccgc
    28321 cgttggcaca cgaacccata gacaataccc agcgcggctc ggcgagctgg tcgtatacgc
    28381 ggcgcagggc gggcgccatt ttgttggtca gcgtacctgc cacaatcatc aggtcggcct
    28441 gacgtgggga cggacggaaa atgataccga aacggtcaag gtcgtaacgc gccatacccg
    28501 catgcatcat ttccacggcg cagcaggcca agccgaaagt aaccggccac aatgaaccgg
    28561 tacgcatata gttcaacacc gtatccgcgc tggtggtgat gaaacctttt ttcaaaacgc
    28621 cttctattcc cattccagcg cacctttttt ccattcgtaa ataaagccta ccgtcagaac
    28681 gacgataaac accagcatag accagaagcc gtatgcgccc aaatctttga acacgacagc
    28741 ccacggcaac atgaacgcga cctccaaatc aaacaggatg aagaggatgg cgacgaggta
    28801 atagcgcacg tcgaacttca tccttgcgtt ttcaaaggct tcaaaaccgc attcgtaagg
    28861 cgcgtctttt tcggcgtagt ggcgtttcgg gcctaaaatc gtgccgagca ggataaacag
    28921 cacgccggct gcaagaccga caaggataaa gacaaagacg ggaaagtaag cggacaacat
    28981 ggttacaccc aaatccgtta acaaaatttc tacaataatt tcgtatttta gcgaatttca
    29041 aaaaccatta aaaggtaaat atcggcaaaa cgcccaaaaa aacccaataa atacaatcat
    29101 gttatgataa cgattcttat ttgattttat aaggtcatat aaatttttac accgtaattc
    29161 gtg
//
LOCUS       DAXHSF010000006        27913 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.006, whole genome shotgun sequence.
ACCESSION   DAXHSF010000006 DAXHSF010000000
VERSION     DAXHSF010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 27913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27913
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.006"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            123..2150
                     /gene="uvrB"
                     /locus_tag="ACFM8S_000206"
     CDS             123..2150
                     /gene="uvrB"
                     /locus_tag="ACFM8S_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFC6313344.1"
                     /translation="MEVIRYPDSPFKLHQPFPPAGDQPSAIAGLLEGLSDGLAYQTLL
                     GVTGSGKTYTMANVIAQSGRPAIIMAHNKTLAAQLYAEMREFFPENAVEYFVSYYDYY
                     QPEAYVPSRDLFIEKDSAINEHIEQMRLSATKNLMTRNDVIIVATVSAIYGIGDPTEY
                     QQMVLSVKEGDTIAQRDIIATLVSMQYERGDLDFKRGSFRVRGDVIDVYPAESSENAL
                     RISLFDDEIDRLDMFDPLSGSLIQRVGRYTVFPSSHYVTPRDTVLRACESIKEELRER
                     IDFFTKENRPVEQQRIEQRTRFDLEMLYEMGFCKGIENYSRHFSGKKEGEPPPTLMDY
                     LPDNAIMFIDESHVTVTQIGGMYKGDASRKQNLVDYGFRLPSARDNRPLKFHEFEKVM
                     PQTVFVSATPAKYEEEHAGQVVEQVVRPTGLVDPQIIIRPVATQVDDLMSEINDRIQK
                     GERVLVTTLTKRMAEQLTDYYSELGIKVRYLHSDIDTVERVEIIRDLRLGLFDVLVGI
                     NLLREGLDIPEVSLVAILDADKEGFLRSHRSLIQTIGRAARNVNGVAILYADKITDSM
                     KAAIDETERRREKQIKFNEEHGIVPQQIKKQVKDIIDGVYHEEDSGKGRRQGKNKVKV
                     GKIHNEEDAIKEIAKLEKAMQQAARDLQFEEAAVLRDRIRGIKEGLLFGAE"
     gene            2234..3130
                     /locus_tag="ACFM8S_000207"
     CDS             2234..3130
                     /locus_tag="ACFM8S_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313345.1"
                     /translation="MLLDNDKIIDPTYKILNFIQSKNRELFDNDGYVILFGNLKTNFL
                     KLFNSVVYVYDIQKDSDFMKRYNQKSFVSSMLIEYSYVKLSTVWDISYQIGAKLTKLT
                     NSKGNRYEQLEKEFLDYAEENSDLNLDWYKEVNKIRNKLVHGGVKIIPFYLDDNGEIK
                     NRLCFQVYDFNLEDLIPISNFYTNKSNNYINFVDNYFAFYTSTLYSYLVDFFNFILLK
                     ICNESCFNLNSFDKHENLDTLERIGIDDKHWSIANMKAFKCIASDMLKLYKNEGNYIG
                     IDRIVINQLELEKFFPMLGNSK"
     gene            complement(3274..3621)
                     /locus_tag="ACFM8S_000208"
     CDS             complement(3274..3621)
                     /locus_tag="ACFM8S_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313346.1"
                     /translation="MTYLKIIITSIVLYILLLQINLKMLEKRIDFLVENIDKYYQQYG
                     SYPNNFDFISTKTDFTTESYCDFWDKNIAGYGNCYFVKNDKDYTILVMGFSSKILFSS
                     HNKIKEFNSNKYE"
     gene            complement(3939..4655)
                     /gene="trmB"
                     /locus_tag="ACFM8S_000209"
     CDS             complement(3939..4655)
                     /gene="trmB"
                     /locus_tag="ACFM8S_000209"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFC6313347.1"
                     /translation="MTDTAENQTPNDRQAGHPRSIRSFVLRQSHMTAAQQRAIDTLWD
                     SFGIDYQATPADLDANFGSNRPKILEIGFGMGTATAEIARRLPETDFLAIDVHGPGVG
                     NLLKLIEENHLENIRVMRHDAVEVVENMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
                     PFIAKLLPKLKTGGYIHLATDWEEYAQQMLEVLSSFDSLQNTAADYAPTPDYRPETKF
                     EARGKRLGHGVWDLVFKRIR"
     gene            complement(4777..6180)
                     /locus_tag="ACFM8S_000210"
     CDS             complement(4777..6180)
                     /locus_tag="ACFM8S_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC6313348.1"
                     /translation="MNNPDLPYRQALERLSQKQYNFTEVRRLLTEAASAGHPAAAFEL
                     AKHLMDADSPYQDREQGMEMLRIAAEQGHPYARYNLAYIQELEGAPPETLIPLYRPLA
                     EEGLPEAQVRLMYLLYASRHFEEALKWAKTSAKNNNPHGQYLLAQYCRYGTPPDFETA
                     HLLYRKAAAQGLAAAHWQLGLQYRFGQGTKVDTAQAVNHLRAAAQQGYIPAYTPLAEL
                     ILPTAPDEAVHWFQQAAQENDPDAHAALADIYLQGKHPERNYKLALHHAEAAAAERHP
                     EGLRILGDIYRYGLGMTSDKEKALHYYRQAAEAGSLAAYQKLISDSALNHPDQYGGIK
                     DSAIRRQRAEQLYQKAQALHYGLQCAPEYAAALKLYTEAAELGHSKAQTNLGSMYYFG
                     QGMTADYNEARKWFEKAAAKKDSMAFYNLACIHYSGHGVGPDKEKACRYLQEAINNGY
                     GQKSVLQELLQQWQNAV"
     gene            complement(6299..8185)
                     /gene="uvrC"
                     /locus_tag="ACFM8S_000211"
     CDS             complement(6299..8185)
                     /gene="uvrC"
                     /locus_tag="ACFM8S_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFC6313349.1"
                     /translation="MPSEAVFQMIDMNTENRSPEQFDIPLFLKNLPKLPGVYRFFDEG
                     GKVLYVGKAVNLKRRVSGYFQKNDHSPRIALMVKQVHHIETTITRSEAEALILENNFI
                     KALSPKYNILFRDDKSYPYLMLSGHQYPQMAYYRGTLKKPNQYFGPYPNSNAVRDSIQ
                     VLQKVFMLRTCEDSVFEHRDRPCLLYQIKRCTAPCVGHISEEDYRDSVREAATFLNGK
                     TDELTRTLQHKMQTAAANLQFEEAARYRDQIQALGIMQSNQFIDSKNPNNPNDIDLLA
                     LAVSDDLVCVHWVSIRGGRHVGDKSFFPDTKNDPDPNGQDYAEAFVAQHYLGKSKPDI
                     IISNFSVPDALKEALEGEHGKQMQFVTKTIGERKVWLKMAEQNAQMAIAQRRLQQSSQ
                     QHRIDELAKILGMDSDGLNRLECFDISHTQGEATIASCVVYDEQNIQPSQYRRYNITT
                     AKPGDDYAAMREVLTRRYGKMLEAEANGESVKWPDVVLIDGGKGQIGVAVSVWEELGL
                     HIPLVGIAKGPERKAGMEELILPFTGEVFRLPPHSPALHLLQTVRDESHRFAITGHRK
                     KRDKARVTSSLGDIPGVGSKRRQALLTRFGGLRGVVAASKEDLEQVEGISKALAETIY
                     NHLH"
     gene            complement(8240..10417)
                     /locus_tag="ACFM8S_000212"
     CDS             complement(8240..10417)
                     /locus_tag="ACFM8S_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFC6313350.1"
                     /translation="MQQKIRFQIEGMTCQACASRIEKVLNKKDFVESAGVNFASEEAQ
                     VVFDDSKTSVADIAKIIEKTGYGAKEKTEDTLPQPEAEHHIGWRLWLLFTINVPFLIG
                     MVGMMLKGLNWTRHDWMLSPLLQFALASVVQLWLAVPFYKSAWASIKGGLANMDVLVT
                     IGTVSIYLYSVYMLFFSPHAAYGMAHVYFEVGIMVIGFVSLGKFLEHRTKKSSLNSLG
                     LLLKLTPTQVNVQRDGEWRQLPIDQVQIGDLIRANHGERIAADGIIESGSGWADESHL
                     TGESNPEEKKAGGKVLAGALMTEGSVVYRAAQLGSQTLLGDMMNALSEAQGSKAPIAR
                     VADKAAAVFVPAVVGIALLTFIVTWLIKGDWTVALMHAVAVLVIACPCALGLATPAAI
                     MVGMGKAVKHGIWFKDAAAMEEAAHVDAVVLDKTGTLTEGRPQVAAVYCVPDSGFDED
                     DLYRIAAAVEQNAAHPLARAIVSATQARGLEIPAAQNAQTVVGAGIAAEVEGAGLVKA
                     GKAEFAELTLPEFSDGVWDIASIVAVSVDNKPIGAFALADALKADTAEAIGRLKKHNI
                     DVYIMSGDNQGTVEYVAQQLGIAHAFGNMSPRDKAAEVQKLKAAGKTVAMVGDGINDA
                     PALAAANVSFAMKGGADVAEHTASATLMQHSVNQLADALLVSQATLKNIKQNLFFAFF
                     YNILGIPLAALGFLNPVIAGAAMAASSVSVLSNALRLKRVKID"
     gene            10556..11506
                     /gene="trxB"
                     /locus_tag="ACFM8S_000213"
     CDS             10556..11506
                     /gene="trxB"
                     /locus_tag="ACFM8S_000213"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFC6313351.1"
                     /translation="MSQHRKLIILGSGPAGYTAAVYAARANLNPVIITGIAQGGQLMT
                     TTEVDNWPADADGVQGPELMARFLAHAERFGTEIIFDQINAVDLQKRPFTLKGDMGEY
                     TCDALIVATGASAKYLGLPSEEAFAGKGVSACATCDGFFYKNQDVAVVGGGNTAVEEA
                     LYLANIAKTVTLIHRRSEFRAEKIMIDKLMKRVEEGKIILKLESNLQEVLGDDRGVNG
                     ALLKNNDGSEQQIAVSGIFIAIGHKPNTDIFKGQLEMDEAGYLKTKGGTADNVGATNI
                     EGVWAAGDVKDHTYRQAITSAASGCQAALDAERWLGSQNI"
     gene            11662..12030
                     /gene="rpsF"
                     /locus_tag="ACFM8S_000214"
     CDS             11662..12030
                     /gene="rpsF"
                     /locus_tag="ACFM8S_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFC6313352.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKTMIAEANGKIHRLEDWGR
                     RQLAYPINKIHKAHYVLMNIETTPEVVEELETAFRFNDAVLRHLTIKTKHAVTEASPM
                     LGGEKAKNLLSGASEEAVAQ"
     gene            12031..12333
                     /gene="priB"
                     /locus_tag="ACFM8S_000215"
     CDS             12031..12333
                     /gene="priB"
                     /locus_tag="ACFM8S_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFC6313353.1"
                     /translation="MGFNNLVSLAVLIEKVFPIRYTPAGIPVLDIILKHESWQEENGQ
                     QCLVQLEIPARILGRQAEEWQYRQGVYVHVEGFLAQKSRRSLMPMLRIQNIQEYKG"
     gene            12340..12570
                     /gene="rpsR"
                     /locus_tag="ACFM8S_000216"
     CDS             12340..12570
                     /gene="rpsR"
                     /locus_tag="ACFM8S_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFC6313354.1"
                     /translation="MARQSFKRRKFCRFTAEKIQEVDYKQVDLLKDFISENGKIIPAR
                     ITGTKAFYQRQLAVAVKRARFLALLPYTDQHK"
     gene            12587..13039
                     /gene="rplI"
                     /locus_tag="ACFM8S_000217"
     CDS             12587..13039
                     /gene="rplI"
                     /locus_tag="ACFM8S_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFC6313355.1"
                     /translation="MQIILLEKIGGLGNLGDIVTVKNGYARNFLIPAGKAKRATEANM
                     KEFEARRAELEAKQAEILADARVRQEKLDGQTITVAQKAGVDGRLFGSVTNADIAAAI
                     VAAGIEAVKANVRLPNGPLKAVGEYEVEVALHTDAVAKITVAVVAATE"
     gene            13053..13160
                     /locus_tag="ACFM8S_000218"
     CDS             13053..13160
                     /locus_tag="ACFM8S_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFC6313356.1"
                     /translation="MPSETLPIVGMWFQTVFVLFSVYSAKIGMVVFIVD"
     gene            complement(13990..14796)
                     /locus_tag="ACFM8S_000219"
     CDS             complement(13990..14796)
                     /locus_tag="ACFM8S_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFC6313357.1"
                     /translation="MWHWDIILILLAVGSAAGFIAGLFGVGGGTLIVPVVLWVLDLQG
                     LAQHPYAQHLAVGTSFAVMVFTAFSSMLGQHKKQAVDWKTVFTMMPGMVFGVFAGALS
                     AKYIPAFGLQIFFILFLTAVAFKTLHTDPQTASRPLPGLPGLTAVSTLFGTMSSWVGI
                     GGGSLSVPFLIHCGFPAHKAIGTSSGLAWPIALSGAISYLLNGLNIAGLPEGSLGFLY
                     LPAVAVLSAATIAFAPLGVKTAHKLSSAKLKKSFGIMLLLIAGKMLYNLL"
     gene            complement(14797..15543)
                     /gene="pssA"
                     /locus_tag="ACFM8S_000220"
     CDS             complement(14797..15543)
                     /gene="pssA"
                     /locus_tag="ACFM8S_000220"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFC6313358.1"
                     /translation="METPTNTPQRSLRQNSIYLLPNSFTIAALFSAFYAITQSMHGRY
                     ETAAIAVFISMLLDGMDGRVARLTNSQSAFGEQLDSLADMVSFGVAPALIAYKWQLWQ
                     FGKIGYSVAFIYCACAALRLALFNTLIGKVDKRWFIGVPSPTAAALIVGLIWVNHSVE
                     KFPAVHWWALGITLFAGLSMIVQIPFWSFKEINIRRQVPFVGMLLAVLLLLLVTWEPS
                     LVLFLFFLGYSLSGYIMAARRFWKKYRKAD"
     gene            complement(15617..15742)
                     /locus_tag="ACFM8S_000221"
     CDS             complement(15617..15742)
                     /locus_tag="ACFM8S_000221"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313359.1"
                     /translation="MPSNNIHPAKIRNNTPGGRQSQTAPQSIRHQKNKPPPRASS"
     gene            16126..16608
                     /locus_tag="ACFM8S_000222"
     CDS             16126..16608
                     /locus_tag="ACFM8S_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HFC6313360.1"
                     /translation="MMKKILAVSALCLMTAAARAADTYGYLAVWQNPQNADDVLQVKT
                     TKEDSTKSEAFAELEAFCKGQDTLAGIAEDEPTGCRSVVSLNNTCVALAYPKALGAMR
                     VESAVVITSPRFTSVHQVALNQCIKKYGAQGQCGLETVYCTSSSYYGGTVRSLIQNLK
                     "
     gene            complement(16660..17871)
                     /locus_tag="ACFM8S_000223"
     CDS             complement(16660..17871)
                     /locus_tag="ACFM8S_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HFC6313361.1"
                     /translation="MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTL
                     LFQITTKRKVPIFLGSSFAFIAPIIYSVGEWGLPSTMFGLFAAGFMYFVFAALIRWRG
                     LAAVHKLLPPVVIGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
                     AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTASIAHAPWFAVPHFETPQINWQA
                     ALFMLPVAVAPAIEHIGGIMAIGNVTGKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPV
                     TTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFG
                     TIASLGVKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFAGVGLCAVLAIM
                     LNSLLPDPKES"
     gene            18094..20532
                     /locus_tag="ACFM8S_000224"
     CDS             18094..20532
                     /locus_tag="ACFM8S_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFC6313362.1"
                     /translation="MTEKSHKKTAKGRAGSPSPTSARNKKADNGARGNKVSERLKAVK
                     ELQKTETKKARPEHVVNLIGDALWLMGLAATLYLAISLISFDMGDPSWSHSSPVVEDV
                     ANWGGLFGAYVADVGYYLFGWSFWWWIAAACVVLYKNFRLHAKQTENEAYNHKIAAAA
                     LFVLTVFSPVLEYFVLGGKYADSLPVGAGGMVGIRVGAVFAWLLGKSGSLLIILVVLL
                     LSLSLLVQISWLEFLNGAGRAVQNRLSALSGKVMALGKRRPNTKTDGVDTQNTRRMVK
                     EAKNITAKPVALPEGSSSNRKSVAVSVAPPPKIQVSLFEDDEPRQAGEYHKPTLNLLR
                     IPDSEPVSINPAELERTAELIESKLAEFGIGVQVVSATSGPVITRYEIEPAQGVKGSQ
                     IVALSKDLARSMSLQSVRIVETIAGKNTMGIELPNDKRQDVMLSEILSSPVFAEAKSK
                     LTVALGKDIAGTPVVGDLEKMPHLLVAGMTGSGKSVGVNGMIMSMLFKATPDEVRFIM
                     IDPKMLELSIYDGIPHLLCPVVTDMREAGQALNWCVAEMEKRYRLLSHAGVRNLEGFN
                     QKVEAAKAAGKPLLNPFSLNPDSPEPLEKLPMIVVVIDELADLMMTERKAVEQQIARL
                     AQKARAAGIHMIVATQRPSVDVVTGLIKANIPTRMAFTVQSKIDSRTILDQMGADELL
                     KYGDSLFLQPGSAEPTRLQGAFVSDDEVHQVVNYVKSQAPADYIEGLLSGEAALETAN
                     IVNPNADSDELFDQAVAYVLESKKTSISSLQRQLRIGYNRAANLMEALENAGVVSPTD
                     LNGSRKILAHKDHL"
     gene            20741..22051
                     /gene="tig"
                     /locus_tag="ACFM8S_000225"
     CDS             20741..22051
                     /gene="tig"
                     /locus_tag="ACFM8S_000225"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFC6313363.1"
                     /translation="MSVTVETLENLERKVVLSLPWSEINAETDKKLKQTQRRAKIDGF
                     RPGKAPLKMIAQMYGASAQNDVINELVQRRFYDVAVAQELKVAGFPRFEGVEEQDDKE
                     SFKVAAIFEVFPEVVIGDLSAQEVEKVTASVGDAEVDQTVEILRKQRTRFNHVEREAR
                     NGDRVIIDFEGKIDGEPFAGGASKNYAFVLGASQMLPEFEAGVVGMKAGESKDVTVNF
                     PEDYHGKDVAGKTAVFTITLNNVSEATLPEVDADFAKALGIADGDVAKMREEVQKNVS
                     REVERRVNEQTKESVMNALLKAVELKAPVALVNEEAARLANEMKQNFVNQGMADAANL
                     DLPLDIFKEQAERRVSLGLILAKLVDENKLEPTEEQVKAVVANFAESYEDPQEVIDWY
                     YAEPSRLQAPTSLAIESNVVDFVLGKAKVNEKALSFDEVMGAQA"
     gene            22147..22761
                     /gene="clpP"
                     /locus_tag="ACFM8S_000226"
     CDS             22147..22761
                     /gene="clpP"
                     /locus_tag="ACFM8S_000226"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFC6313364.1"
                     /translation="MSFDNYLVPTVIEQSGRGERAFDIYSRLLKERIVFLVGPVTDES
                     ANLVVAQLLFLESENPDKDIFFYINSPGGSVTAGMSIYDTMNFIKPDVSTLCLGQAAS
                     MGAFLLSAGEKGKRFALPNSRIMIHQPLISGGLGGQASDIEIHARELLKIKEKLNRLM
                     AKHCGRDLADLERDTDRDNFMSAEEAKEYGLIDQILENRASLQF"
     gene            complement(22817..24082)
                     /gene="ispG"
                     /locus_tag="ACFM8S_000227"
                     /gene_synonym="gcpE"
     CDS             complement(22817..24082)
                     /gene="ispG"
                     /locus_tag="ACFM8S_000227"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFC6313365.1"
                     /translation="MNTLQRRKTHQVRIDHITVGSEAPVVIQSMTNTDTADAKATALQ
                     IKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPE
                     CGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
                     ANLASSAPKPPEEVTKEALIVSALESAEKAVLLGLPEDKIILSCKVSAVQDLIQVYRE
                     LGSRCAYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEV
                     VVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYLRQKMSIWRTLYPGV
                     ESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKVTLKGDNIATEFL
                     AIVEEYVKTNYGENGSKRNQNKIIPIQSL"
     gene            complement(24098..24859)
                     /gene="pilW"
                     /locus_tag="ACFM8S_000228"
     CDS             complement(24098..24859)
                     /gene="pilW"
                     /locus_tag="ACFM8S_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HFC6313366.1"
                     /translation="MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAM
                     EYMRGQDYRQATASIEDALKSDPKNELAWLVRAEIYQYLKVNDKAQESFRQALSIKPD
                     SAEINNNYGWFLCGRLNRPAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLA
                     EAYLKRSLAAQPQFPPAFKELARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWK
                     IAKALGNAQAAYEYEAQLQANFPYSEELQTVLTGQ"
     gene            complement(24862..25956)
                     /gene="rlmN"
                     /locus_tag="ACFM8S_000229"
     CDS             complement(24862..25956)
                     /gene="rlmN"
                     /locus_tag="ACFM8S_000229"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFC6313367.1"
                     /translation="MKTNLLNYDLQGLTRHFADMGEKPFRAKQVMRWMHQSGAQNFDE
                     MTDLAKSLRHKLNEQADIEIPKLMMSQKSSDGTRKWLLDVGTGNGVETVFIPESDRGT
                     LCISSQVGCALECTFCSTGRQGFNRNLTAAEIIGQLWWANKAMGVTPKNERVISNVVM
                     MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMVPQMDRLRDVMPVALAVS
                     LHASNDEVRNQIVPLNKKYPLKELMAACQRYLVKAPRDFITFEYVMLDGINDKAQHAR
                     ELIELVTDVPCKFNLIPFNPFPNSGYERSSNENIRVFRDILQQAGFVVTVRKTRGDDI
                     DAACGQLAGQVQDKTRRQQKWQQILIGQQG"
     gene            complement(26100..26525)
                     /gene="ndk"
                     /locus_tag="ACFM8S_000230"
     CDS             complement(26100..26525)
                     /gene="ndk"
                     /locus_tag="ACFM8S_000230"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFC6313368.1"
                     /translation="MAIERTISIIKPDAVGKNVIGKIYSRFEENGLKIVAAKMKQLTL
                     KEAQEFYAVHKDRPFYAGLVEFMTGGPVMIQVLEGENAVLKNRELMGATNPSEAAEGT
                     IRADFATSVSINAVHGSDTPENAALEIAYFFSQTEICPR"
     gene            complement(26584..27735)
                     /gene="zapE"
                     /locus_tag="ACFM8S_000231"
     CDS             complement(26584..27735)
                     /gene="zapE"
                     /locus_tag="ACFM8S_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFC6313369.1"
                     /translation="MSSRDQLFKAPPFENHSPLTWYQAASQLPNFIRDDAQTAAIEHL
                     DRLWTELMMFKRKRNRFLGRSLRSPQVPKGLYFYGGVGRGKSFLMDAFFGCLPYRRKR
                     RVHFHAFMAEIHQRLKTLKSESNPLKSVAAEIAKETRVLCFDEFHVSDIADAMILGRL
                     LENLLNEGVVLVATSNYAPSELYPQGQNRSSFLPTIALIESSLTVLNVDGGEDYRLRT
                     LRPAEIFFTPANEENEAKLAKLFKEMTGITDLNPGISTIHGREIPHKAESGRAIWFDF
                     RALCFGPRSQSDYLYLAEHYEMVFISGLEQLSPQEKAEARRLTWLIDVLYDFRVKLCA
                     TGAVDVNHIYTEGDFAEEFTRTASRMVEMQSEVYLEQPHLTLSPKASGG"
BASE COUNT         6836 a         6782 c         7371 g         6924 t
ORIGIN      
        1 tgggtagcga ttgggtttca gacggcatac attcaggatt tcaccgcctt atgccgtctg
       61 aaagacgttt ccgacccatc cctactacac gctttgtccg caaacgcatc acaggcaaga
      121 ttatggaagt cattcgatac cccgattccc ccttcaaact ccaccaaccc ttcccgcccg
      181 caggcgacca gccctccgcc attgccggcc tgctcgaagg gctttcagac ggccttgcct
      241 atcaaaccct gcttggcgta accggttcgg gcaaaaccta caccatggcg aacgtcatcg
      301 cccaaagcgg ccgacccgcc atcatcatgg cgcacaacaa aacccttgcc gcccagcttt
      361 atgccgaaat gcgcgagttt ttccccgaaa acgcggtgga atatttcgtc tcctactacg
      421 actattacca acccgaagcc tatgtgccca gccgcgactt gttcatcgaa aaagacagcg
      481 cgatcaacga acacatcgag cagatgcgcc tttccgccac caaaaacctg atgacgcgca
      541 acgacgtgat tatcgtcgcc accgtgtccg ctatttacgg tatcggcgac ccgaccgagt
      601 atcaacaaat ggtgttgtcc gtcaaagaag gcgataccat agcccagcgc gacatcatcg
      661 ccacgctcgt ttccatgcag tacgaacgcg gcgatttgga cttcaaacgc ggcagcttcc
      721 gcgtgcgcgg cgacgtgatt gacgtgtacc ccgccgaaag ctccgaaaac gccttgcgta
      781 tcagcctgtt cgacgacgaa atcgaccgcc tcgatatgtt cgacccgctt tcaggcagcc
      841 ttatccaacg cgtcggccgc tacaccgtct tcccgtccag ccactacgtt accccgcgcg
      901 acaccgtatt gcgcgcctgc gagtccatca aagaagaatt gcgcgaacgc attgatttct
      961 ttaccaaaga aaaccgtccc gtcgagcaac aacgcatcga acagcgcacc cgtttcgacc
     1021 tcgaaatgct ctacgaaatg ggcttctgca aaggtatcga aaactactcc cgtcacttct
     1081 ccggcaaaaa agaaggcgaa ccgccgccca cgctgatgga ctacctgccc gacaacgcca
     1141 tcatgttcat cgacgaaagc cacgttaccg tcacacaaat cggcggcatg tacaaaggcg
     1201 acgcatcgcg caagcaaaac ctcgtcgatt acggcttccg cctaccttcc gcccgcgaca
     1261 accgcccgct caaattccac gaatttgaaa aagtcatgcc gcaaaccgtc ttcgtttccg
     1321 ccactcccgc caaatacgaa gaagaacacg ccggacaagt ggtcgaacaa gtcgtccgcc
     1381 ccacagggtt ggtcgatccc caaatcatca tccgccccgt cgccacccaa gtcgacgact
     1441 taatgagcga aatcaacgac cgaattcaaa aaggcgaacg cgtactcgtt accaccctca
     1501 ccaaacgcat ggcggaacaa ctcaccgact attacagcga actcggcatc aaagtgcgct
     1561 acctgcacag cgacatcgac accgtcgagc gcgttgaaat cattagagat ttacggctcg
     1621 gactgtttga cgtactcgtc ggtatcaacc tcttgcgtga aggcctcgac atccccgaag
     1681 tctccctcgt cgccatcctc gatgccgaca aagaaggctt cctgcgctcc caccgcagcc
     1741 tgattcaaac cataggccgc gctgcgcgca acgtgaacgg cgtcgcgatt ctgtacgccg
     1801 acaaaatcac cgactctatg aaagccgcca tcgacgaaac cgaacgccgc cgtgaaaaac
     1861 aaattaaatt caacgaagaa cacggcatcg tgccgcaaca gattaaaaaa caggtcaaag
     1921 acatcattga cggcgtgtac cacgaagaag acagcggcaa aggccgtaga caaggcaaaa
     1981 acaaagttaa agtcggcaag attcacaacg aagaagacgc gattaaagaa attgccaaac
     2041 tggaaaaggc tatgcagcag gcagctaggg atttacagtt tgaagaggcg gctgtgttga
     2101 gggataggat tcgggggatt aaggaagggt tgttgtttgg ggcggagtaa aaaagataac
     2161 ggaagaaatt caaaatgtct tagcgagtta cttaaaattg atcaattaca ataaagtttg
     2221 aaaggtgtaa ataatgttat tagataatga taaaattatt gatccaactt ataaaattct
     2281 taattttata caaagtaaaa acagagaatt attcgataat gatggctatg taatattgtt
     2341 tggaaattta aaaactaatt ttttaaaatt atttaattcc gttgtctatg tttatgatat
     2401 acaaaaagat agtgatttta tgaaaagata caatcaaaaa tcttttgtta gctcaatgtt
     2461 aattgagtat tcatatgtta aattgagtac agtttgggat atttcatatc aaatcggtgc
     2521 taagttaacc aaattaacaa actcaaaagg gaatagatac gaacagctag aaaaagagtt
     2581 tttagattat gcagaagaaa atagtgattt aaatttagat tggtataagg aggttaataa
     2641 aataagaaat aaattagttc atggtggagt taaaataatc cctttttatt tagatgataa
     2701 tggggaaata aaaaatagat tatgttttca agtatatgat tttaatctgg aagatttaat
     2761 acctataagt aatttttata ctaataaatc gaataactat ataaattttg tagataatta
     2821 ctttgctttt tatacaagta ctttatactc atatttagtt gattttttca attttatttt
     2881 attaaaaata tgtaatgaaa gctgctttaa tttaaatagt tttgataaac atgaaaattt
     2941 agatactttg gaacgaattg gaatagatga taagcactgg tcaatagcca atatgaaggc
     3001 atttaaatgt attgcttctg atatgctaaa attatataaa aatgaaggaa attatattgg
     3061 tattgataga atagttatca atcaattaga attagagaaa tttttcccta tgcttggaaa
     3121 cagcaagtaa gcgtagtcgg atacttgtat ccgacaaaat ctttaacgcc cccatcattg
     3181 caattcattc agcaatgcct aaaatgtcgg attcaagaat ccgacctacg gaaatctaaa
     3241 aattttacgc gggctacggc ttgcttattg tatttattca tatttatttg aattgaattc
     3301 ttttatttta ttgtgagatg aaaatagtat tttcgatgag aaacccatga ctaaaatagt
     3361 atagtcttta tcattcttta cgaagtaaca attaccatat cctgcaatat ttttatccca
     3421 aaaatcacaa tatgattcag tagtaaaatc agttttagtt gatataaaat caaaattatt
     3481 tgggtaagac ccatattgct ggtaatattt atctatattt tctactaaga aatcaatcct
     3541 tttttctaac attttgagat taatttgtaa taataaaatg tataaaacta tactagttat
     3601 tattattttt aaataagtca ttttattttc cagtaaatag aggtttagtt gttttgttaa
     3661 ctatgcctga atgtacatct aagtcttctt ttggtaacga tgaataacta gtaagccgta
     3721 gcctgcatct ccgccaaccg cgtgccacta tctgcgggcg gaataaactt taaaaccgat
     3781 gtgtataaaa cctacaagga tgtgtgcggt acacacgcgt tttttcgttc aacccattgt
     3841 tttaaaactt gtatcgaaat atcactcgct gctttatctt gaaaaaatgc cgtctgtaag
     3901 caaacgtgct tcagacggca ttttcaaacg gcggtttgtt atctgatccg tttgaatacc
     3961 aagtcccaaa cgccgtgtcc aaggcgtttg ccgcgcgctt cgaatttggt ttcggggcgg
     4021 tagtccgggg tgggggcgta gtctgccgcc gtattttgca ggctgtcgaa gctgctgagg
     4081 acttcaagca tctgctgcgc atattcttcc cagtctgtcg ccaggtggat atagccgccg
     4141 gttttgagtt tgggcagcag tttggcgatg aagggggctt gtatcagacg gcgtttgtgg
     4201 tggcgttttt tgtgccacgg gtcggggaag aatatgtgga tgccgtcgag cgagccgtct
     4261 tgcagcatat tttcgacaac ttctacggcg tcgtgccgca tcacgcggat attttccaaa
     4321 tggttttctt ctatgagttt gagcaggttg cctacgccgg gaccgtgtac gtcgatggcg
     4381 agaaagtcgg tttcgggcag cctgcgggcg atttctgcgg ttgctgtccc cataccgaag
     4441 cctatttcga ggattttggg gcggttgctt ccgaaattgg catcaagatc ggccggtgtt
     4501 gcttggtaat cgatgccgaa gctgtcccat aaggtatcga tggcgcgttg ctgcgcggcg
     4561 gtcatatggc tttggcggag gacgaaactg cggatgctgc ggggatgtcc ggcttgacgg
     4621 tcgtttggtg tttggttttc ggcggtgtct gtcataatgc ttccgtgcgc ttgtgcaatc
     4681 aggatgccgt ctgaatgtga aaggcctgtt cggacggcgg gaagggaaag gggcgattat
     4741 acctgtttcg tttcggcagg gtaggtgtaa cgctgttcag acggcatttt gccattgttg
     4801 cagcagttct tgcaggacgc ttttttgccc gtatccgttg tttatggctt cttgcaggta
     4861 gcggcaggct ttttctttgt ccggcccgac accgtgtccg ctgtaatgga tgcaggcgag
     4921 gttgtagaac gccatactgt ctttttttgc ggcggctttt tcaaaccatt tgcgtgcttc
     4981 attgtagtcg gcggtcatac cctgtccgaa gtaatacatg ctgcccagat tggtttgggc
     5041 tttgctgtgt ccgagttctg cggcttctgt gtagagtttg agcgcggctg cgtattcggg
     5101 cgcgcattgt aatccgtaat gcagggcttg ggctttttga taaagctgtt ctgccctttg
     5161 ccgcctaatg gcggaatctt taatgccgcc gtattggtca ggatggttta acgcgctgtc
     5221 ggatatgagt ttctgatacg cggcaaggct gccggcttcg gctgcctgcc ggtaataatg
     5281 cagggctttt tctttatcgg aagtcatgcc cagtccgtag cggtagatgt cgcccagtat
     5341 ccgcaaacct tcgggatggc gttcggcggc ggctgcttcg gcgtgatgca gggcaagttt
     5401 gtagtttctt tccggatgct tgccttgcag gtagatgtcg gcaagtgcgg catgggcatc
     5461 ggggtcattt tcctgtgccg cctgttgaaa ccagtgaacg gcttcatcag gagccgtagg
     5521 taggatgagc tcggcaagcg gggtgtaggc gggaatgtat ccttgttgtg cggcggcgcg
     5581 caaatgattg acggcctgtg ccgtgtcgac tttcgtccct tgtccgaaac ggtattgcag
     5641 cccgagctgc cagtgtgccg ccgccaagcc ttgtgccgcc gctttgcggt agagcaggtg
     5701 cgccgtttca aaatccggcg gcgtgccgta ccggcagtat tgggcaagca ggtattgccc
     5761 gtgggggttg ttgttttttg cgcttgtttt tgcccatttc agggcttctt caaaatgtcg
     5821 ggacgcatac agaaggtaca tcaggcggac ttgtgcttca ggcagtcctt cctctgccaa
     5881 cggtctgtaa agcggtatca gggtttccgg gggcgcgcct tccaattctt ggatatatgc
     5941 cagattgtaa cgcgcatagg gatgcccctg ttcagcggcg atgcggagca tttccatacc
     6001 ttgttcgcgg tcttggtagg ggctgtccgc gtccatcagg tgttttgcca actcgaatgc
     6061 ggcggcggga tgacctgccg aggccgcttc tgtcagcagt cggcggactt cggtaaagtt
     6121 atattgtttt tgagacaggc gttctaaggc ctgccgatag ggtaagtcgg gattgttcat
     6181 gtcagatcgt tttccgtttt gtcgaatcat cgttattgtg ttatgaagtt aggtttatat
     6241 cggcacgccg acctactgta tcatattttt tacagacggt ttttgcttgg gtggcacact
     6301 aatgcagatg attgtaaatc gtttccgcca atgccttgct gatgccttcg acttgttcca
     6361 aatcttcttt gctggcggca acgacgccgc gcaggccgcc gaagcgggta agcagggctt
     6421 ggcggcgttt gctgcctacg ccgggaatgt cgccgaggga cgaggtaacg cgtgctttgt
     6481 cgcgtttttt gcggtggcct gtaatggcaa agcggtgtga ttcgtcgcgc acggtttgca
     6541 gaaggtgcag ggcggggctg tgcgggggca ggcggaagac ttcgccggta aaaggcagta
     6601 tgagttcttc cataccggct ttgcgctccg ggcctttggc gataccgacc aaggggatgt
     6661 gcagcccgag ttcttcccat accgatacgg ctacgccgat ttgccctttg ccaccgtcaa
     6721 tcaacacgac atccggccat ttgacgcttt cgccgttggc ctcggcttcc agcattttgc
     6781 cgtaacggcg cgttaggact tcgcgcatag cggcgtagtc gtcgccgggt ttggcggtgg
     6841 tgatgttgta gcggcggtat tgcgaaggct ggatgttttg ctcatcgtac acaacgcagg
     6901 acgcaatagt ggcctcgcct tgtgtgtggc tgatatcgaa acattcaagg cggttgagac
     6961 cgtctgaatc catgccgagg atttttgcca gttcatcaat acggtgttgc tggctacttt
     7021 gttgcaggcg gcgttgtgca atcgccattt gtgcgttttg ttccgccatt ttcaaccaga
     7081 ctttgcgttc gcctatggtt ttggtgacaa attgcatctg cttgccgtgt tcgccttcca
     7141 aagcctcttt cagcgcatcg ggaacggaaa agttgctaat aatgatgtcg ggtttgcttt
     7201 tgcccaaata atgttgggcg acgaaggctt cggcgtaatc ttgtccgttt ggatcgggat
     7261 cgtttttggt gtcggggaaa aagcttttgt cgccgacgtg tcgtccgccg cggatgctga
     7321 cccagtgtac gcagaccagg tcgtctgaaa ccgccagcgc gagcaaatcg atatcgttgg
     7381 gattgttcgg atttttgctg tcgataaact gattactctg catgatgccg agtgcttgga
     7441 tttgatcgcg gtaacgggct gcttcctcaa attgcagatt ggcggcggcg gtttgcattt
     7501 tgtgttgcag ggtacgcgtc agttcgtcgg ttttgccatt aagaaaagtc gcggcctcgc
     7561 gcacgctgtc gcgataatct tcttcgctga tatgaccgac acaaggcgcg gtgcagcgtt
     7621 tgatttggta aagcagacaa ggacggtcac gatgctcgaa tacactgtct tcgcaggtac
     7681 gcagcataaa gactttttgt aatacttgaa tgctatcgcg cacggcattg ctgttgggat
     7741 acgggccgaa atattgattt ggctttttca gtgtgccgcg gtaatacgcc atctgcggat
     7801 attgatggcc gctgagcatc aaataaggat agcttttgtc gtcgcggaaa aggatattgt
     7861 atttcggcga caaggctttg atgaagttgt tttcgagaat cagggcttcg gcttcggaac
     7921 gcgtgatggt ggtttcgata tgatgaacct gttttaccat caatgcgatg cgcggcgaat
     7981 ggtcgttttt ttggaaatag ccggacacgc gccgcttgag gttgaccgct ttgccgacgt
     8041 ataagacttt gccgccttcg tcaaaaaaac ggtacacgcc cggcagcttg ggcaggtttt
     8101 tgaggaagag cgggatgtcg aattgttccg gagaacggtt ttctgtattc atatcaatca
     8161 tttgaaaaac ggcttcagac ggcatatcgg cagcggcagc cttatccgtt cggaaaagct
     8221 gtcgggtggt tatatgcggt caatcgattt ttacccgttt caggcgcaag gcattgctca
     8281 acacggaaac cgagcttgcc gccatggccg cgcctgcgat gacgggattt aaaaagccaa
     8341 gcgcggcgag cggaatgccc aagatattgt agaagaaggc gaaaaacagg ttttgcttga
     8401 tgtttttcaa agtggcttgc gacaccagca gggcatcggc gagctgatta accgaatgct
     8461 gcatcagcgt ggcggacgcg gtgtgttcgg caacgtccgc accgcctttc atggcgaagc
     8521 tgacgtttgc ggcggcgagc gcgggcgcgt cgttaatgcc atcgccgacc atcgctacgg
     8581 ttttgccggc ggctttgagt ttctgcactt cggcggcttt gtcgcgcgga ctcatattgc
     8641 cgaaggcgtg agcgataccc agttgttggg cgacgtattc gaccgtgcct tggttgtcgc
     8701 cgctcatgat gtaaacatcg atattgtgtt ttttcagacg gcctatggct tcggcggtat
     8761 cggctttcaa cgcgtcggca agtgcgaatg caccgatagg tttgttatcg actgagactg
     8821 caacaatgct tgcaatgtcc caaacgccgt ctgaaaactc gggcaaggtc agttcggcaa
     8881 attcggcttt gcccgctttc accaatcccg caccttccac ttcggcggca atgcctgcgc
     8941 cgacaacggt ttgcgcgttt tgtgcggcgg gaatctccaa accgcgcgct tgggtggcgg
     9001 aaacgatggc tcgggcaagc ggatgggcgg cgttttgttc gacggcagcg gcgatgcggt
     9061 acaaatcgtc ttcgtcaaag ccgctgtcgg gaacacaata aacggcggca acctgcggcc
     9121 tgccttcggt cagcgtgcca gttttgtcca acacaacggc atcgacgtgg gcggcttctt
     9181 ccattgccgc tgcgtcttta aaccaaatgc cgtgtttcac tgctttaccc atgccgacca
     9241 taatcgcggc gggtgtagcc agaccgagcg cacacgggca ggcaatcacc aaaacggcga
     9301 cggcgtgcat cagcgcaacc gtccaatcgc ccttaatcag ccaagtaaca ataaaagtca
     9361 acaacgcgat gcccacgacg gcaggcacga acaccgccgc cgccttgtcc gccacgcgcg
     9421 caatcggtgc tttactgcct tgcgcttcag agagtgcgtt catcatgtcg ccgagcaggg
     9481 tttggctgcc gagctgcgcg gcgcggtaca ccacgctgcc ttcggtcatc agcgcgcccg
     9541 ccaacacttt gccgcccgcc tttttctctt cgggattgga ttcgccggta agatggctct
     9601 cgtccgccca gccgctgccg ctctcgatga tgccgtcggc ggcaatgcgt tcgccgtggt
     9661 tggcgcggat taggtcgccg atttgcactt ggtcgatggg tagctgccgc cattcgccat
     9721 cgcgttgcac gttgacttgg gttggcgtga gtttgagcag caagcccaag ctgttcaggc
     9781 tggatttttt ggtgcggtgt tccaaaaatt tacccagtga cacaaaacca atcaccatta
     9841 tgcctacttc aaaatacaca tgcgccatac cgtacgccgc gtgcgggctg aaaaacagca
     9901 tatagacgga atacaggtaa atcgagaccg tgccgatggt aacgagtacg tccatattcg
     9961 ccagcccgcc tttaatgctc gcccacgcgc ttttgtaaaa tggcaccgcc agccaaagct
    10021 gcaccacact cgccaatgca aactgcaaca agggcgacaa catccaatca tgccgtgtcc
    10081 aattcagccc ttttagcatc atccctacca tgccgataag gaacgggacg ttgatggtga
    10141 acagcagcca cagccgccag ccgatatggt gttctgcttc gggttgcggc aatgtatctt
    10201 ccgttttttc cttcgcgccg taaccggttt tctcaatgat tttggcaatg tcggctactg
    10261 aggttttgct gtcgtcaaac actacctgcg cctcttcgct ggcgaagttt acccccgccg
    10321 attcgacaaa atcttttttg ttcaacactt tttcaatgcg cgaagcgcag gcctggcagg
    10381 tcatgccttc gatttggaaa cggatttttt gttgcataag cgtgattcct gctttgatat
    10441 tcagacggca gttaaggctg cgggatgccg tctgagcgcc ggtatggtcg aaatttctca
    10501 aacgcgccga taattcgagt agaatccgaa aacttgacac agcaaaggaa aaacaatgtc
    10561 ccaacaccgc aaactgatta ttttgggttc cggccccgcc ggatacaccg ccgccgtcta
    10621 tgccgcgcgc gccaatttaa accccgtcat tattacaggt atcgcgcaag gcgggcaact
    10681 gatgaccacc accgaagtgg acaactggcc tgccgatgcc gacggcgtgc aagggccgga
    10741 attgatggcg cggtttctcg cccacgccga acgtttcgga acggaaatca tttttgacca
    10801 aatcaacgcc gtcgatctgc aaaaacgccc gtttacgctc aaaggcgata tgggcgagta
    10861 cacttgcgat gccctgattg tcgcaacagg cgcgtccgcc aaatacctcg gtttgccgag
    10921 tgaggaagcg tttgcaggaa aaggcgtttc cgcttgtgcc acctgcgacg gtttcttcta
    10981 taaaaaccaa gatgttgccg tagttggcgg cggcaatacg gcagttgaag aggcactcta
    11041 ccttgccaat atcgccaaaa ccgttaccct gatccaccgc cgcagcgagt tccgtgccga
    11101 aaaaatcatg atcgacaaac tgatgaaacg cgtggaagag ggcaaaatca tcctcaagct
    11161 ggaaagcaac ctgcaagaag tactgggcga cgatcggggc gtaaacggcg cattattaaa
    11221 aaacaacgac ggttctgagc aacaaattgc cgtcagcggc atttttatcg ccatcgggca
    11281 caagccgaat accgatattt tcaaaggtca gttggaaatg gacgaagccg gctacctgaa
    11341 aaccaaaggc ggcacggcgg acaatgtcgg tgcaaccaat atcgaaggcg tatgggcggc
    11401 gggcgacgta aaagaccata cctaccgtca ggcaattacc agcgcggctt ccggctgtca
    11461 ggcggctttg gacgcggaac gctggctggg cagccaaaac atttgaatgc cgtctgaaac
    11521 cgaaattttc ggcggaatgc aaatttttcg caccaaatcc ttgattccgt cggcaaaccc
    11581 caatataatg cctgctcttt gaaccttgcc ttctgctttc gcatggcaga aggctgtttt
    11641 taccatccat aaggagataa catgcgtcat tacgagatcg tgtttatcgt tcatcctgat
    11701 caaagcgagc aagtgcctgc tatggttgaa cgttacaaaa ccatgattgc cgaagcaaac
    11761 ggtaagattc accgtttgga agattggggt cgccgccaac tggcttaccc gattaacaaa
    11821 atccataaag cccattacgt tttgatgaac atcgaaacca ctcccgaagt ggttgaagag
    11881 ctggaaaccg cattccgctt caatgatgcc gtattgcgcc atctaaccat caaaaccaaa
    11941 cacgccgtta ccgaagcatc ccctatgttg ggtggtgaaa aggctaagaa cctgttgagc
    12001 ggcgcgtctg aagaagcggt cgcccaataa ttgggattca ataatcttgt ttcgcttgcc
    12061 gtgttaattg aaaaggtttt ccctattcga tatacgcctg ccggaatccc tgttttagat
    12121 attattttaa agcacgaatc gtggcaggag gaaaacgggc agcaatgcct tgtccaattg
    12181 gaaattccgg cacggatttt aggcaggcag gcggaagagt ggcagtatcg gcaaggtgta
    12241 tatgttcacg tcgaaggttt tttagctcaa aaaagcagac gttcccttat gccgatgctc
    12301 aggatacaaa atattcaaga atataaaggt taaacgacaa tggctcgtca atcattcaaa
    12361 cgtagaaaat tctgccgttt cacggctgaa aaaatccaag aagtcgatta caaacaagtt
    12421 gatttgctga aagactttat ctctgaaaac ggtaaaatca ttcctgcacg catcacagga
    12481 acgaaggcat tctaccaacg ccaattggct gttgccgtaa aacgcgcacg cttcctggct
    12541 ctgctgcctt acaccgacca acacaaataa ttttggagat tgaatcatgc aaattattct
    12601 gttagaaaaa atcggcggtc tgggcaactt gggcgacatc gtaaccgtta aaaacggcta
    12661 cgcccgcaac tttctaattc ccgcaggtaa ggcaaaacgt gcgaccgaag cgaatatgaa
    12721 agagtttgaa gcacgccgtg ccgaattgga agccaaacag gctgaaattt tggcagatgc
    12781 ccgagtccgt caggaaaaat tggacggtca aaccattacc gttgcacaaa aagccggtgt
    12841 ggacggtcgc ctgttcggtt ccgtcaccaa tgctgatatt gcggctgcaa tcgttgctgc
    12901 cggcatcgaa gccgtgaaag caaatgtacg tctgccgaac ggtcctctga aagccgttgg
    12961 cgagtacgaa gtggaagtgg ctttgcacac tgatgccgtt gctaaaatta ccgttgccgt
    13021 cgttgccgca accgagtaat tgaaattatc agatgccgtc tgaaaccctg ccgattgtcg
    13081 gaatgtggtt tcagacggta tttgttttgt tttccgtata ttctgcaaaa atcgggatgg
    13141 ttgtttttat agtggattaa atttaaacca gtacagcgtt gcctcgcctt gccgtgctat
    13201 ctgtattgtc tgcggcttcg tcgccttgtc ctgatttttg ttaatccact atatttgcct
    13261 gtggtctttg caggaatagt ttgttaacga aatttgatgc ataaaatgcg ccaaaaaata
    13321 ttggctgtgt tttaatatag tgttgataag cgtacctggc ttcaatatac tcaataggat
    13381 agatctcttc ctaaccgaaa aaataattgt cttttccctt gcaaataatt gaaatcaaca
    13441 gattactaaa acacagccaa aatattcaat tgacaaaaac ttttctgatt ttgagaaatc
    13501 tgtgaaaatc aggggattat ttattttgaa aatggtatcg ggcataaaat ttggctgtgt
    13561 ttcaatatgg tgctgataac atactttgtt tcaaaatgtt caataggata acttcctgat
    13621 tgaccgaaaa ataatggcct tttcccttgc aagcagttga aatcaacaga ttgtcaaaat
    13681 acatcctaaa aatttaataa cttatatata tgattgcaaa ggttgcggct tgtatgccgg
    13741 tcattatggc ggaaataccg tttgggaggc agccgcatcg cgtggaatcg gcacggcagg
    13801 cgatgcgccg atgccttcgg gcgtgaaggc ggggcaatcg gcagtttgga aaaaggcaat
    13861 ggaatctgtg ggcagaagaa gttaagggta aaagtcgggc gcacggtcag gttgtctgtc
    13921 cttttgtcgg aacaggagac tgggcagctt tgtcctaatt aattgcttgt gcaaacggta
    13981 aaaaggtttt taaagcaggt tgtacagcat ttttccggca atcaaaagca acataatgcc
    14041 gaaggatttt ttgagtttgg cagaagaaag tttgtgggcg gttttgacac cgagcggggc
    14101 aaaggcaatg gttgccgcgc tgaggacggc gacggcgggc aggtaaagga agcccagtga
    14161 cccttcgggc aatcctgcaa tattcaggcc gttgagcaga tacgatattg cgccggagag
    14221 tgcaatcggc caggcaaggc cggatgatgt gccgatggct ttatgggcgg ggaagccgca
    14281 gtggattaag aaggggacgg aaagtgaacc gccgcctatg ccgacccagc tcgacattgt
    14341 gccgaacagt gtggaaaccg cagtcagtcc gggcagtccg ggcagcgggc gggatgccgt
    14401 ctgagggtcg gtatgcagtg ttttgaatgc gacggcggtt aaaaacagga tgaagaaaat
    14461 ttgaagcccg aacgctggga tatattttgc ggagagtgcg ccagcgaata cgccgaatac
    14521 catgcctggc atcatcgtaa atacggtttt ccagtcgacc gcctgttttt tgtgctgccc
    14581 cagcatactg gaaaaggcgg tgaagaccat gacggcgaag gatgtgccga cggcgaggtg
    14641 ttgcgcgtaa ggatgttgtg ccaaaccctg caaatcaagt acccataaaa cgacagggac
    14701 aatcagcgtg ccgccgccta cgccgaacag accggcaata aaacctgccg cactgcctac
    14761 ggcaagcagg attaagataa tgtcccaatg ccacatttaa tccgcctttc tgtatttttt
    14821 ccagaatcgg cgtgccgcca taatgtagcc ggacaggctg tatccgagaa agaacaggaa
    14881 gaggacgagc gacggttccc aagtgaccag aagcagcaat aagacggcaa gcagcattcc
    14941 gacaaagggg acttgtctgc ggatgttgat ttctttaaaa ctccaaaaag ggatttggac
    15001 aatcatcgac aggccggcaa acagtgtgat gcccaatgcc caccagtgga cggcggggaa
    15061 tttttcgacg ctgtggttga cccaaatcag cccgacaatc agcgcggcgg cagtcggact
    15121 gggcacgccg ataaaccagc gtttgtccac cttgccgatg agtgtgttga acagggcgag
    15181 gcgcagggcg gcgcaggcgc agtagatgaa ggcgacggaa taaccgattt tgccgaactg
    15241 ccaaagctgc catttgtagg caatcagagc gggagcaacg ccgaagctga ccatatcggc
    15301 aaggctgtcg agctgctccc cgaacgcgct ttggctgttg gtcagccgcg ccacgcgccc
    15361 gtccataccg tccagcaaca tagagatgaa taccgcgatg gcggcggttt cataacgtcc
    15421 gtgcatggat tgggtgattg cgtaaaatgc ggaaaacagc gcggcgatgg taaaggagtt
    15481 gggcagcagg tagatactgt tttgacgcag ggagcgttgc ggggtgttgg tcggggtttc
    15541 catagctgaa gtgtctcacg gacgtggaga atcgataggg gattataacc caaaccatta
    15601 tcgaacaata cgccctttag gatgaagccc tcggaggcgg tttgtttttc tgatggcgga
    15661 tgctttgcgg ggcggtttga ctctgcctgc caccgggtgt gttgtttcgg atttttgccg
    15721 gatggatatt gttgctgggc atttctgggc ttgccggaag gtatcggggg gagggcatcg
    15781 gaatatgcgg cgcaccgttc aaaccggggc ggcgtttccc tattttcagg gggttgcagg
    15841 tttcaacaaa ttgaaagata ttttttgcat ttccgaccgt atgctgattt tgaagcggga
    15901 taaattatag gacaaggttt ttcgcccgcc gcaaaggttg gccgcaatgc cgtctgaaaa
    15961 caacggcttg ccgctaaggc ggcggtatgc ggatttgcgc tgccgaaatc ggtttgccat
    16021 acccggcggt gcaatctgcg gggagggata agccgataag gcgggactta tcctgttttt
    16081 tgtttataat gcaacagaat ttggacgaac cgtcgggaat ttatcatgat gaaaaagata
    16141 ctggccgtat cggcactatg cctgatgact gcggcggcac gggctgccga tacttacggc
    16201 tatctcgccg tttggcagaa tccgcagaat gcggacgatg ttttgcaggt taaaaccaca
    16261 aaagaagatt cgacgaaaag cgaagcgttt gccgagttgg aagctttctg caaaggtcag
    16321 gacacgcttg cgggcattgc cgaagacgag ccgaccggat gccggtcggt cgtgtcgctg
    16381 aacaatacct gtgtcgcgct ggcatacccg aaagccttgg gcgcaatgcg cgttgaaagc
    16441 gccgtcgtga ttacttctcc gcgttttacg agcgttcatc aggtcgcact caaccagtgc
    16501 atcaaaaaat acggagcaca gggacaatgc ggtttggaaa cagtgtattg cacatcttct
    16561 tcttattacg gcggaactgt gcgctctttg attcaaaatc tcaaataaaa cggaaaatgc
    16621 cgtctgaaag atgttcagac ggcatttcta tatcgacggt caggattctt tcggatcggg
    16681 cagcaggctg ttcaacataa tggcaagtac ggcgcacaag cccacgccgg caaagctgaa
    16741 gctgcccaat ttgagcgtca tgccgccgat gcccgtggtc agtaccgagc tgacgatgac
    16801 caggtttttc ggcagcatca aatcgacttt ggcatcaatc agcgttttca cgcccaaaga
    16861 agcaatcgtg ccgaacagca gcagcataat gccgcccatt actggcatcg gaatggaagc
    16921 caaaaacgca ttgaatttgc cgaaaaacgc catgcagacg gcaaaaaccg ccgcccaagt
    16981 catgatgacg gggttgctgt ttttggtaat catcaccgca cccgttactt cgccgtaggt
    17041 cgtaaccggc gggccgccga tcagacccgc aacgcacacg cccaaaccgt cgcccgcaag
    17101 ggttttgtcc aagcccgggt ctttcgtata gtctttcccc gtcacattgc cgattgccat
    17161 gatgccgccg atgtgttcga tggcgggggc gacggcaacg ggcagcataa acagtgcagc
    17221 ctgccagttg atctgaggcg tttcaaaatg gggaacggcg aaccagggcg cgtgtgcaat
    17281 gcttgccgtg tccaccagtc ccatcagcag tgccaaaaca taacccgaag cgacaccgat
    17341 caagatggga atcagcttca tcatcctgct gccgaaaacc gatacgatgg cggtaacggc
    17401 aaaggtaaag ccggaaagaa tcagtgaatc ggtatagtcg atgacctgtt tgccgtccgc
    17461 ctgacccatt gccatgctgc ttgccgccac ggcaacagac agaccgatga ccatgatgac
    17521 ggggccgatg acgaccggag gcagcagttt gtgtaccgct gccagtccgc gccaacggat
    17581 cagcgcggca aacacaaaat acataaagcc ggcggcaaac agtccgaaca tggtggaagg
    17641 cagcccccat tcgccgacgg agtagataat cggtgcgata aaggcaaacg aagaaccaag
    17701 aaaaatcggc actttgcgtt tggttgtgat ttggaacagc agcgttccca agcctgcgcc
    17761 caaaagcgca agagccggat tcagaccggt cagcagggga accagcacca ttgcgccgaa
    17821 tgccacaaat aaaatctgtg caccggaaac ggcaagtttc agttggttca taccatcttc
    17881 tttcaatcgg ttcaaacggc acggattata ctggtttgcc gagggtctgc gtaaaaatac
    17941 atacgtcggg caacagatat gccgtctgaa aggcaatgtc tgtctgttgc aggatgaaag
    18001 caggagaaat ggagtataat ccgacacttt ccatattcaa tccgcaagcc gtccggcttc
    18061 cttatttcag acggcatttt ccggtaatac ggcatgacag aaaaatccca taaaaaaaca
    18121 gcaaaaggca gggcgggaag cccgtcccca acgtccgcac gcaacaagaa agccgacaac
    18181 ggcgcacggg gcaataaagt ttccgagcgg ctcaaggcgg tcaaagagtt gcagaaaacc
    18241 gaaaccaaaa aggcgcgtcc cgaacatgtc gtcaacctta tcggcgacgc actgtggctg
    18301 atgggtttgg cggcaaccct gtatttggcg atttccctga tcagtttcga tatgggcgat
    18361 ccgtcttggt cgcacagttc gccggttgtg gaagatgtcg ccaattgggg cggactgttc
    18421 ggcgcgtatg ttgccgatgt cggctattat cttttcggct ggtcgttctg gtggtggata
    18481 gcggctgcct gcgtcgtgct gtataaaaat ttccgcctgc acgcaaaaca gacggaaaac
    18541 gaggcataca accacaaaat cgctgccgcc gcgctgtttg tcctgacggt cttcagcccc
    18601 gtcttggagt attttgtgct gggcggaaaa tatgccgact ccctgcctgt cggagcaggc
    18661 ggtatggtcg gcatacgcgt cggcgcagtg tttgcgtggc tgctggggaa atcgggcagc
    18721 ctgctgatta tcttggttgt tctgctgttg tcgttgtccc tactggtgca gatttcatgg
    18781 ctggaatttt tgaacggtgc aggcagggcg gttcaaaacc gcctgagtgc cttatccggc
    18841 aaggtcatgg ctttaggaaa acgccggccg aataccaaaa cagacggtgt cgatacccaa
    18901 aatacacggc gcatggtaaa agaagccaag aatattacgg ccaaacccgt tgccttgccc
    18961 gaaggcagca gcagcaaccg caaatccgtc gcggtttccg tcgcgccgcc gcccaaaatt
    19021 caggtttctc tgtttgaaga tgacgaacct cggcaggcgg gcgaatacca caagcctaca
    19081 ttgaacctat tgcggattcc tgacagcgaa cccgtcagca tcaatcccgc cgaattggag
    19141 cgcactgccg aactgatcga atccaaactg gcagaattcg gcatcggcgt acaagtcgta
    19201 tccgccacat ccggtcccgt catcacgcgc tacgaaatcg aacccgcgca aggtgttaaa
    19261 ggcagccaaa ttgttgcctt gtcgaaagat ttggcacgct ctatgtcgct gcaatccgtg
    19321 cgtatcgtcg aaaccatcgc aggtaaaaac acgatgggca tcgagttgcc caacgacaaa
    19381 cgccaagacg tgatgttgag cgaaatcttg tcctcgcccg tgtttgccga agccaaatcc
    19441 aagctgaccg tcgcgctggg caaagacatt gccggtaccc ccgttgtcgg cgacttggag
    19501 aaaatgccgc accttttggt cgccggtatg actggttcgg gcaagtccgt cggcgtgaac
    19561 ggcatgatta tgtctatgct tttcaaagct acgcccgacg aagtccgctt tatcatgata
    19621 gacccgaaaa tgcttgagtt gagcatttac gacggcattc cgcacctgct ctgtcccgtc
    19681 gtgaccgata tgcgcgaagc ggggcaggcg ttgaactggt gcgtcgccga aatggaaaaa
    19741 cgctaccgcc tgctttccca cgccggtgta cgcaatctgg aaggcttcaa ccaaaaagtc
    19801 gaagccgcaa aagcggcagg caagccgctg ctcaatccgt tcagtctgaa ccccgacagc
    19861 cccgagcctt tggaaaaact gccgatgatt gtggtcgtta tcgacgaact tgccgacctg
    19921 atgatgaccg aacgcaaagc cgtcgagcag caaatcgccc gtctcgccca aaaagcgcgc
    19981 gccgccggta tccatatgat tgtcgccacc caacgtccca gtgtcgatgt cgttactggc
    20041 ctgattaaag ccaacatccc gacgcgtatg gcgtttaccg tgcaaagcaa aatcgacagc
    20101 cgtaccatcc tcgaccaaat gggcgcggac gaactgctca aatatggcga ttcgctgttc
    20161 ctccagcccg gcagtgccga accgacccgc ctgcaaggcg cgtttgtttc agacgacgaa
    20221 gtacatcaag tcgtcaacta cgtcaaatcg caagccccag ccgactatat tgaaggtctg
    20281 ctcagcggcg aggccgcgct ggaaactgcc aatatcgtta atccgaatgc agacagcgac
    20341 gaattgttcg atcaggcagt cgcctatgtt ttggaaagca aaaaaacctc catttcgtct
    20401 ttgcagcggc agttgcgtat cggctataac cgcgcggcaa acctgatgga ggcactggaa
    20461 aatgcgggtg tcgtttctcc caccgacctc aacggcagcc gtaaaatttt ggcgcacaag
    20521 gaccatttgt agcccgtatt gcaaaatgcc gtctgaacgg cggaattggc gtttcagacg
    20581 gcatattatg tttcaggcgg aacattgtga tatacttgcc agctaaaatc tcccctttgc
    20641 ggcaatgcgg ttcaaatatc cgtaccgttg cgctgtttgc ttccccatgc agggaagaag
    20701 gtttaccatt ttatcaacac aacaaattta agggcttatg atgagcgtaa ctgttgaaac
    20761 tttagaaaat ctggaacgca aagtagtgtt gtccctgcct tggtccgaaa tcaacgcaga
    20821 aaccgataaa aaactgaaac aaacccagcg ccgtgcaaaa atcgacggtt tccgcccggg
    20881 taaagcacct ttaaaaatga ttgcccaaat gtacggtgcg agcgcgcaaa acgacgtgat
    20941 caacgagctg gtgcaacgcc gcttctacga tgttgccgtt gcccaagagt tgaaagtggc
    21001 aggcttcccc cgttttgaag gcgttgaaga acaagacgat aaagagtctt tcaaagttgc
    21061 cgccattttt gaagtgttcc ccgaagtcgt tatcggcgat ttgtctgcac aagaagttga
    21121 aaaagtaacc gcttccgtcg gtgatgccga agtggaccaa accgtagaaa tcctgcgcaa
    21181 acaacgcacc cgcttcaacc atgtcgaacg cgaagcccga aacggcgacc gcgtcatcat
    21241 tgactttgaa ggcaaaatcg acggcgaacc ttttgcaggc ggcgcatcca aaaactacgc
    21301 cttcgtattg ggcgcaagtc aaatgctgcc tgaatttgaa gccggcgtag tcggcatgaa
    21361 ggctggcgaa agtaaagacg ttaccgtcaa tttccctgaa gactaccacg gtaaagacgt
    21421 tgccggtaaa actgccgtgt tcaccattac gctgaacaac gtttccgaag cgactctgcc
    21481 tgaagtcgat gcagattttg caaaagcttt gggtattgcg gatggcgacg ttgccaaaat
    21541 gcgcgaagaa gtgcagaaaa acgtaagccg cgaagtggaa cgccgcgtaa acgaacaaac
    21601 caaagaatcc gtaatgaacg cgctgctcaa agccgtagag ctgaaagcac ctgttgcttt
    21661 ggtcaatgaa gaagccgcac gcttggcaaa cgaaatgaaa caaaattttg ttaaccaagg
    21721 tatggctgat gctgccaact tggatctgcc tttggatata ttcaaagaac aagccgaacg
    21781 ccgcgtgtct ttgggtctga ttttggccaa actggttgat gaaaacaaac tggaaccgac
    21841 tgaggagcaa gtcaaagccg ttgtcgccaa cttcgcagaa agctacgaag atcctcaaga
    21901 agtaatcgac tggtactacg cagagccttc ccgcctgcaa gccccgactt ctttggcgat
    21961 agaaagcaac gtcgttgatt tcgttttggg taaagccaaa gtaaacgaaa aagctttgtc
    22021 ttttgacgaa gtgatgggtg cgcaagcctg attatcctga aaatgccgtc ttagggcagt
    22081 ttttgaaagc accgaagccg cttttaaagc ccgctttggt gcttttacat catgaaagga
    22141 gacgaaatgt cttttgataa ctatcttgtc cctaccgtta tcgagcagag cggtcgcggt
    22201 gagcgtgcat tcgatatcta ttcccggctt ttgaaagagc gcatcgtatt cttggtcggt
    22261 ccggtaaccg acgagtccgc caatctggtg gttgcccaac tgttgttttt ggaaagtgag
    22321 aatccggata aggatatttt cttctatatt aactcgccgg gcggttcggt aacggccggt
    22381 atgtcgattt acgacaccat gaattttatc aagcccgatg tatcgacttt gtgcttgggg
    22441 caggcagcaa gtatgggcgc gttcttattg tcggcaggcg aaaaaggcaa acgtttcgcc
    22501 ctgcccaaca gccggattat gattcaccag cctttaatca gcggaggctt gggcggtcag
    22561 gcatccgata ttgaaattca cgcacgcgaa ctgttgaaaa tcaaagaaaa actcaaccgc
    22621 ctgatggcga aacattgcgg ccgcgatttg gcagatttgg agcgcgacac cgaccgtgat
    22681 aatttcatgt ccgccgaaga ggctaaagaa tatggtctga ttgatcaaat cttggagaac
    22741 cgtgcttctt tacagtttta ataagaaatc tgagagaaac aaatgccgtc tgaaaacagt
    22801 tcagacggca tttgtcttat agggactgta ttgggatgat tttgttctgg ttgcgtttgg
    22861 agccgttttc gccgtagttg gttttgacat actcttcaac aatagccaaa aattccgttg
    22921 caatgttgtc gcctttcagt gtgactttgc gctccccgtc cacataaacc ggcgcgacgg
    22981 gtgtttctcc cgtaccgggc aggctgatgc cgatgtcggc caatttgctt tctccgggcc
    23041 cattgacaac gcagcccatt acggcaacgt tcagggattc aaccccggga taaagggtac
    23101 gccatataga cattttttgg cgcaggtaat tttgaacatc ttgtgccagc tcttgaaata
    23161 cggtactggt ggtacgcccg cagccggggc aggcggtaac catcggcgta aacgaacgca
    23221 atcccatagt ctgtaaaatc tcttgcccga cgacgacctc ctgagtacgc gggctgccag
    23281 gttccggagt cagtgaaatg cggatggtgt cgccgattcc ttcttgaagc aagacggata
    23341 atgccgccgt tgatgcgaca atgcctttgc tgcccatacc ggcttcggtc aaacccaaat
    23401 gcagcggata ggcgcaacgg ctgcccagtt cgcggtaaac ctgaatcaaa tcctgaaccg
    23461 cgctgacttt gcacgacagg atgattttgt cttcgggcag tcccaataga acggcttttt
    23521 cggcagattc caaagcggag acaatcagtg cttccttcgt cacttcttcg ggcggtttcg
    23581 gcgcggaaga agcgaggttg gcatccatca tacgtttggc gaggctttga tccaaagaac
    23641 cccagtttac gccgatgcgg acggctttat cgttttcagc agcggtccga atcataaagg
    23701 caaatttttc atcgcctttt acgcctttgc cgacattgcc gggattgatg cggtatttgg
    23761 acaatgcttt gccgcattcg ggaaattccg ccaacaggcg ttcgccgttg aagtggaaat
    23821 cgccgataag tggtgtggca tagcccatat cgtccaagcg gcggcggatt tcggcaactt
    23881 tggacgcggc ttcggggctg ttgacggtaa tacgcaccat ttcggatccg gcatcgctca
    23941 attccttaat ctgcaatgcg gtggcttttg catcggcagt gtcggtgttg gtcatagatt
    24001 ggataacgac gggtgcttct gaaccgacgg taatatgatc gatgcggact tgatgcgtct
    24061 tgcggcgttg gagtgtgttc atatggtttg aatctgttta ttgaccggtg aggacggttt
    24121 gcaattcttc cgagtagggg aaattcgcct gcaattgtgc ttcatattcg tatgccgcct
    24181 gtgcgttgcc gagggctttg gcaattttcc agcctagcag caaatcatcg gcctgaagga
    24241 cttctaccct gctttggtat tttttaaagt agtaatcggc atcgcccaac tgcccggcca
    24301 gcattttggt gcgcgccagt tctttaaatg cgggtgggaa ctgcggctgg gcggcgaggg
    24361 aacgtttcaa ataggcttcc gccaatccga attgcccctg ttttgcgctg catatgcctt
    24421 tattcaggtt ggcaatataa ggggtcgggt aggtggggtc ggccagggct ttgtcgaaat
    24481 atgccataga ttcggcaggg cggttgagcc tgccgcacag gaaccagccg tagttgttgt
    24541 tgatttcggc actgtcgggt ttgatggaga gggcttgccg gaaactttcc tgcgccttgt
    24601 cgttaacttt caggtattga tagatttcgg cacggaccag ccaggcaagc tcgtttttag
    24661 ggtctgattt caaggcgtct tcaatacttg ccgtcgcctg acggtagtcc tgaccgcgca
    24721 tatattccat tgccaactgg gttttgatat tggaaacctg attggctttt tctgcccgcg
    24781 aggggcggta ggaagtgctg cacgcgccca aggcaaggac aagtaataaa gagattcgtt
    24841 tggatggctt aaaaggcata attacccctg ttgtccgatt aaaatctgct gccatttttg
    24901 ttggcggcgc gttttatcct gaacctgccc cgccaactgt ccgcaggcgg catcgatgtc
    24961 gtcgccgcgc gtttttcgta cggtaacgac aaatcctgcc tgctgcaaaa tatcgcggaa
    25021 cacacggatg ttctcattgc tggagcgttc gtatccggag tttgggaagg gattgaacgg
    25081 aatcagattg aacttgcagg gaacatctgt gaccagttcg atcagttcgc gcgcatgttg
    25141 cgccttatcg tttattccgt ccaacatgac gtattcgaaa gtgatgaaat ccctgggtgc
    25201 tttgaccaga tagcgttggc atgcggccat caattctttc aagggatatt ttttgttcaa
    25261 cggtacgatt tggttgcgga cttcgtcatt ggaagcgtgg agggaaaccg ccaaagccac
    25321 cggcatgaca tcgcgcaacc tgtccatttg gggaaccata cccgaagtgg aaacggttac
    25381 gcggcggcgg ctcaaaccgt agccgtggtc gtccagcatg atgcttaagg cggtaacgac
    25441 attgtcgaag ttcgccatcg gctcgcccat gcccatcatg acgacgttgg aaataacgcg
    25501 ctcgtttttc ggtgtaacgc ccatcgcttt gtttgcccac cacaattgcc cgatgatttc
    25561 ggcagcagtc aaattgcggt tgaagccctg ccgaccggtc gaacaaaatg tacattccaa
    25621 agcgcagccg acttgtgagg aaatgcagag cgtgccgcga tccgattcgg ggatgaagac
    25681 ggtttccacc ccgttgcccg taccgacatc caaaagccat tttcgagtgc cgtctgaaga
    25741 tttttgagac atcatcagct tgggaatttc gatgtctgcc tgttcgttca gtttatggcg
    25801 caacgatttt gccaaatcgg tcatttcgtc aaaattttgc gcgccggatt ggtgcatcca
    25861 acgcataacc tgtttggcac ggaaaggttt ttcgcccata tcggcaaaat gtcgggtcag
    25921 cccttggagg tcgtagttga gcagattggt tttcatgtat ttgtgttttt cttaaattaa
    25981 ggcttcaaat aaaaaattgc agggcaggca agtttgattt gaagcctttt gcaacagagt
    26041 tttcagacgg catggatata ttttatgccg tctgaagagg agttgtgcgg tgtattgtat
    26101 caacgggggc agatttcggt ttggctgaaa aagtaggcaa tttccaaagc ggcattttcc
    26161 ggagtgtccg agccgtgtac ggcattgatg ctgaccgaag tggcaaagtc cgcgcgtatc
    26221 gtgccttcgg cggcttcgga aggattagtt gcacccatca gttcgcggtt tttcagaacg
    26281 gcgttttcac cctctaatac ctgaatcata accggaccgc cggtcataaa ttcaaccaat
    26341 ccggcgtaga aggggcggtc tttatgaacc gcataaaatt cttgcgcctc tttgagggta
    26401 agctgcttca ttttggcggc aacgattttc agaccgttct cctcaaagcg gctgtatatt
    26461 ttgccgataa cgtttttgcc gacggcatcg ggtttgatga tggagatggt acgttcaatc
    26521 gccatgctat atccttattt ttacttaaga agaatcaaat cggtattcta tcaaaaaaat
    26581 aacttatccg cctgaagcct tgggagatag ggtcaggtgc ggctgttcca aataaacttc
    26641 ggactgcatt tcgaccatcc ggctggcggt gcgggtaaat tcttcggcaa aatcgccttc
    26701 tgtatagata tggttgacat ctaccgcgcc ggtggcacac agtttgacac ggaaatcgta
    26761 gagtacgtca atcagccaag tcagccgccg tgcctccgcc ttttcttgcg gtgagagttg
    26821 ttccaaacct gaaataaaaa ccatttcata atgttcggcc aaatacaggt agtcggactg
    26881 tgagcggggg ccgaagcaca gtgcgcggaa atcaaaccat atggcacggc cggactcggc
    26941 tttgtgggga atctcccgac cgtggatggt gctgatgccg gggttcaaat cggtaatgcc
    27001 tgtcatttct ttgaacagtt ttgccagttt tgcctcattt tcttcattgg caggcgtaaa
    27061 gaaaatctcg gcggggcgga gggtacgcag tcggtagtct tcaccgccgt caacgtttaa
    27121 gacggtcagg ctggactcga tgagcgcgat tgtgggaaga aaactgctcc ggttttgacc
    27181 ttgcgggtag agttcggaag gcgcgtagtt tgaagtcgcc accaaaacaa cgccctcgtt
    27241 aagcaggttt tccagcagac ggcctaaaat cattgcatcc gcaatatcgc tgacatgaaa
    27301 ttcgtcaaaa cataatacgc gggtttcttt ggcaatctcg gcggcaacgg atttcaacgg
    27361 gttgctttcg cttttcaggg ttttcagcct ctggtggatt tctgccataa aggcatgaaa
    27421 gtggacgcgg cgtttgcggc ggtaagggag gcagccgaaa aaagcgtcca tcagaaagct
    27481 tttgccgcgt ccgaccccgc catagaaata aagccctttg gggacttgcg gggagcgcaa
    27541 actcctgcct aaaaaacggt ttcttttgcg tttgaacatc atcaattcgg tccaaagccg
    27601 atcgaggtgt tcgatggcgg ctgtctgcgc gtcgtcgcgg atgaagttgg gcagttgtga
    27661 ggcagcctga taccaggtca gcgggctgtg gttttcaaac ggcggggctt taaaaagttg
    27721 gtctctacta ctcatattta acctttttat acttcttgcg gctaagaaag aaaatgctta
    27781 ctgtcggtat tataaatgat ttccttcaaa cttggcattt tccgtggaaa agaataatga
    27841 cgcgcacaag ggggtatgag caaaggcggc gtttcaatat ggtgcagata tgagctcaaa
    27901 tgccttttat agt
//
LOCUS       DAXHSF010000007        27588 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.007, whole genome shotgun sequence.
ACCESSION   DAXHSF010000007 DAXHSF010000000
VERSION     DAXHSF010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 27588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27588
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.007"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(66..905)
                     /locus_tag="ACFM8S_000232"
     CDS             complement(66..905)
                     /locus_tag="ACFM8S_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HFC6313370.1"
                     /translation="MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVD
                     ATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNG
                     DWAPRIAQGFDTLFQHALNGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDE
                     AAPADNAASGTASAPADSAAPAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPG
                     IPGIGKKDDWAPRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSG
                     AKF"
     gene            1190..1265
                     /locus_tag="ACFM8S_000233"
     tRNA            1190..1265
                     /locus_tag="ACFM8S_000233"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1223..1225,aa:Arg,seq:ccg)
     gene            complement(1330..2523)
                     /locus_tag="ACFM8S_000234"
     CDS             complement(1330..2523)
                     /locus_tag="ACFM8S_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFC6313371.1"
                     /translation="MYRHIEYYPGDPILSLVETFKNDPRPEKVNLSIGIYFDDEGKMP
                     VLESVSRAETARAAAPAPSPYLPMEGLDAYRSAVQHLLFGKDNPALAQGRIVTVQTLG
                     GSGALKVGADFLHRWFPEARAYVSDPTWDNHRGIFEGAGFEVGTYPYYDPATVGVKFN
                     EMTAFFNTLPENSVLILHPCCHNPTGVDMSEQQWDEVLHIIKRRKLIPFMDIAYQGFG
                     GDLDSDAYAVRKAVEMELPLFVSNSFSKNLSLYGERVGGLSVVCPNKEEADLVFGQLK
                     FTVRRIYSSPPAHGAYIAADVMNSPELYVLWQNEVYMMRDRIRAMRQKLYDVLTAQIP
                     DRDFTYFIKQRGMFGYTGLSVEQVRRLRDEFAVYLLDSGRMCVAGLNTSNTDYVARAF
                     AEVLK"
     gene            complement(2544..3632)
                     /gene="trmA"
                     /locus_tag="ACFM8S_000235"
     CDS             complement(2544..3632)
                     /gene="trmA"
                     /locus_tag="ACFM8S_000235"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFC6313372.1"
                     /translation="MSDYTQQLQDKKDHLKTLFAGLDVPEWEVYESPDKHYRMRAEFR
                     IWHEGGEMFYAMFEKGQKAGGASLIRCDRFEAASEAVNRLMPELIAVAAQSAELRNRW
                     YAVEFLSTLSGEMLVTMIYHKRLDNEWMQAAQALQQQLDISVIGRSRGQKIVLKQDYV
                     TETLKVGNRDFRYRQIEGSFTQPNAAVCQKMLEWACGAAEGLDGDLLELYCGNGNFTL
                     PLSEKFERVLATEISKTSVSAAQWNIEANRIGNIKIARLSAEEFTEAYTGKREFKRLK
                     DGGIALTDYAFSTIFVDPPRAGIDEETLKLVSQFDNIIYISCNPETLRTNLDTLAETH
                     AVERAALFDQFPFTHHIESGVLLKKKIL"
     gene            3775..4875
                     /gene="aroC"
                     /locus_tag="ACFM8S_000236"
     CDS             3775..4875
                     /gene="aroC"
                     /locus_tag="ACFM8S_000236"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFC6313373.1"
                     /translation="MAGNTFGQLFTVTTFGESHGAGLGCIIDGCPPGLALTESDIQAD
                     LDRRKPGTSRHVTQRREADQIEILSGVFEGKTTGTPIALLIRNTDQRSKDYGNIAQSF
                     RPGHADYTYWHKYGTRDYRGGGRSSARETAARVAAGAVAKKWLKEKFGTEITAYVTQV
                     GEKEIRFEGCEHISQNPFFAANHSQIAELENYMDSVRKSLDSVGAKLHIEAANVPVGL
                     GEPVFDRLDAEIAYAMMGINAVKGVEIGAGFDSVTQRGSEHGDELTPQGFLSNHSGGI
                     LGGISTGQDICVNIAIKPTSSIATPRRSIDINGNPIELATHGRHDPCVGLRAAPIAEA
                     MLALVLIDHALRHRAQNADVAVDTPDIARSDK"
     gene            4966..5391
                     /locus_tag="ACFM8S_000237"
     CDS             4966..5391
                     /locus_tag="ACFM8S_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFC6313374.1"
                     /translation="MTQETALGAALKSAVQTMSKKKQTEMIADHIYGKYDVFKRFKPL
                     ALGIDQDLIAALPQYDSALIARVLANHCRRPRYLKALARGGKRFDLNNRFKGEVTPEE
                     QAIAQNHPFVQQALQQQSAQAAAETPSVEAEAAESSAAE"
     gene            complement(5465..7450)
                     /gene="parE"
                     /locus_tag="ACFM8S_000238"
     CDS             complement(5465..7450)
                     /gene="parE"
                     /locus_tag="ACFM8S_000238"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFC6313375.1"
                     /translation="MAKNNQYSESSITVLKGLEPVKERPGMYTRTDSPTHICQEVIDN
                     AADEALGGFATAIDVQIHEDGSLSVRDNGRGIPVGLHPEEGVPVVELVFTRLHAGGKF
                     NKKDGGSAYAFSGGLHGVGVSVTNALSTRLEVTVKREGKIHRIVFAGGDVVEPLAQVG
                     KCAVKDSGTEVRVWPDGKYFESPNYSIPELERLLRAKAVLLPGVRVSLTRPVKGEDEA
                     HTQTWHYPDGLKSYLTDLIADAQEAVPLFSCENYISDEHNGDFSIGEGAAFALTWLEE
                     GSCANESYVNLIPTPLGGTHEAGLKQAVFNAVSNFINLHNLLPRGVKVQSDDVFGKTA
                     FVLSARVLDPQFQGQTKDKLTNRDALKLVATVSGDPLELWLNQNVDFGKKIAELAIRQ
                     AQARMRSVKKIEKKKGSGVAVLPGKLTDCESEDIRENELFLVEGDSAGGSAKLARDKA
                     TQAILPLRGKVLNSFEVHPDQLFGNAEIHDISVAIGVDPHGINDHPDLSGLRYGKIAI
                     LSDADVDGSHIQVLLLTLFYRHFPKLVADGHIYVAQPPLFRVDVNAQGKSKPARKFYA
                     LDQNELDSILERLQKEGVKETAYSISRFKGLGEMNPDQLKDTTMHPDTRRLLQVQIPE
                     GADDETRDIFVKLMGKGEAAARRAWMEREGDTAELDI"
     gene            complement(7517..8065)
                     /locus_tag="ACFM8S_000239"
     CDS             complement(7517..8065)
                     /locus_tag="ACFM8S_000239"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFC6313376.1"
                     /translation="MNLTEGDTVLDREGYRPNVGIILINERNEVFWGKRVREHSWQFP
                     QGGIKPGESPETAMYRELYEEVGLLPQHVKIVGRTRDWLRYDVPNNWVRREWRGSYRG
                     QKQIWYLLRLTGRDCDVNLRATRHPEFDGWRWHQYWAPVDEVIDFKRDVYLGALKELS
                     SRFLRGMESYEDFAARQLSGNR"
     gene            8206..9501
                     /gene="serS"
                     /locus_tag="ACFM8S_000240"
     CDS             8206..9501
                     /gene="serS"
                     /locus_tag="ACFM8S_000240"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFC6313377.1"
                     /translation="MLDIQLLRSNTAAVAERLAARGYEFDAARFNALEEQRKAVQVKT
                     EELQASRNSISKQIGALKGQGKHEEAQAAMDQVAQIKTDLEQAAADLDAVQKELDAWL
                     LSIPNLPHESVPAGKDETENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSG
                     ARFTVMRGQIARLHRALAQFMLDTHTLQHGYTEHYTPYIVDDTTLQGTGQLPKFAEDL
                     FHVTRGGDETKTTQYLIPTAEVTLTNTVADSIIPSEQLPLKLTAHSPCFRSEAGSYGK
                     DTRGLIRQHQFDKVEMVQIVHPEKSYETLEEMVGHAENILKALELPYRVITLCTGDMG
                     FGAAKTYDLEVWVPAQNTYREISSCSNCEDFQARRLKARFKDENGKNRLVHTLNGSGL
                     AVGRTLVAVLENHQNADGSINIPAALQPYMGGVAKLEVK"
     gene            complement(9574..10572)
                     /locus_tag="ACFM8S_000241"
     CDS             complement(9574..10572)
                     /locus_tag="ACFM8S_000241"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFC6313378.1"
                     /translation="MKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDVKTAKMAEG
                     AEAVCIFVNDDGSRPVLEKLAQIGVKTVALRCAGFNNVDLKAAEELGLKVVRVPAYSP
                     ESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATM
                     RILKGFGMNLLAYDPFCNPEAEKIGGKYVDLDELYAQSDIITLHCPSTPENHYMLNEA
                     AFDKMKDGVMIINTSRGGLIDSSAAIEALKRRKIGALGMDVYENERELFFEDKSNDVI
                     TDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVGQTGDCGNAVRADG"
     gene            complement(10697..11773)
                     /gene="prfA"
                     /locus_tag="ACFM8S_000242"
     CDS             complement(10697..11773)
                     /gene="prfA"
                     /locus_tag="ACFM8S_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFC6313379.1"
                     /translation="MKPSILEKLQQLGDRLEEVTHLLGQPEATSDMDNYRKLTREHAE
                     LTPVVEVFQNYRLAQSDLADAEEMLSDPEMKDFAAEEIEAAKAKIGALDTELQKLLLP
                     KDADDDKNIFIEVRAGTGGDEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
                     EVIARIIGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELN
                     PVDLRIDTFRASGAGGQHINKTDSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAA
                     RLNDAQKREAQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMD
                     GDLAEITDALIAEHQAELLAAMGD"
     gene            complement(11864..12634)
                     /locus_tag="ACFM8S_000243"
     CDS             complement(11864..12634)
                     /locus_tag="ACFM8S_000243"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFC6313380.1"
                     /translation="MNFTDTHCHLADAALRENLPHILTAARKVGVKRFIVPATCPQDW
                     QSVAELSEMPSEHGQIRIALGIHPWFSDGIAERDCVRLEAMLARYPQAWVGEIGLDFY
                     DKTQTPPQRERQIQVFVRQLAIAQTLRRRVIIHNLKATADIAAAVKQTGFAQGGIVHA
                     FSGSAEEARVLTKLGFKIGIGSLLLNPNARKVRDTLKALNDGDFVLETDSPFMLKNTV
                     NTPANILRIAEIAAQIRGTGAAEIAAITERNADSLLRP"
     gene            complement(12749..13738)
                     /locus_tag="ACFM8S_000244"
     CDS             complement(12749..13738)
                     /locus_tag="ACFM8S_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFC6313381.1"
                     /translation="MKQKIFVLYTGGTIGMTQSSEGLRPDTALVGQALAPFSNGMDFD
                     WHVCGTLIDSSAVQLQNWCAWLDIIAAALPQYDGVLVLHGTDTLAYTANLFALALQGL
                     GKPIVLTGSQWPYNAVGSDAPRNLATAVAAFPLGLKQTLIAFDGKLYPAVGSSKVSTE
                     TAAGFDNPHFGALAEWDETQGWHNVRIPNQDAADVSDGLKIRYPDPQAKIAVRTLIPG
                     FAVQELADGLGQLPAQALILQSYGHGNTPADEGFIRAVQDFTQQGKLLLNISQVRQGR
                     TAAVYAQGNAFRNSGIINGGKCNLETATALMTLAVSQGWGKEEVEKELARLKL"
     gene            complement(13805..14488)
                     /locus_tag="ACFM8S_000245"
     CDS             complement(13805..14488)
                     /locus_tag="ACFM8S_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFC6313382.1"
                     /translation="MFALLEAFFVEYGYAAVFFVLVICGFGVPIPEDLTLVTGGVISG
                     MGYTNPHIMFAVGMLGVLVGDGIMFAAGRIWGQKILKFKPIARIMTPKRYAQVQEKFD
                     KYGNWVLFVARFLPGLRTAVFVTAGISRKVSYLRFLIMDGLAALISVPVWIYLGEYGA
                     HNIDWLMAKMHSLQSGIFIALGVLAAALAWFWWRKRRHYQLYRAQLSEKRAKRKAEKA
                     AKKAAQKQQ"
     gene            complement(14677..16011)
                     /gene="glmM"
                     /locus_tag="ACFM8S_000246"
     CDS             complement(14677..16011)
                     /gene="glmM"
                     /locus_tag="ACFM8S_000246"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFC6313383.1"
                     /translation="MAKKYFGTDGVRGEVGQFPITPDFVLKLGYAAGQVLVQHDTDQK
                     PTVLIGKDTRISGYMLEAALVAGFTAAGVNVVQTGPLPTPGVAYLTRALRLSAGVMIS
                     ASHNAYSDNGIKFFAEGGVKLSDEVELEIEAKIDGEMKTQPSARLGRARRINGADDRY
                     IEFCKSTFPTHSDLRGLKLVVDAANGAAYSVAPKVFHELGAQVVSIGDEPNGYNINEK
                     CGATYTKTLQAAVLQHEANYGIALDGDGDRLMMVDKNGKVYDGDSLIYVIAKARAREG
                     INIGGVVGTVMTNMAMEIALKEQGVDFCRAKVGDRYVLEQLNQRGWLVGGEASGHILC
                     MDKHNTGDGIISALQVLAALQTLNQDLAAVCADWQPYPQTMINVRIGKGQNWQEASKD
                     VLAEVEKELEGKGRVVLRASGTEPVVRVMVEARQADWAREGAERIAAAIGGI"
     gene            complement(16141..16992)
                     /gene="folP"
                     /locus_tag="ACFM8S_000247"
     CDS             complement(16141..16992)
                     /gene="folP"
                     /locus_tag="ACFM8S_000247"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFC6313384.1"
                     /translation="MARHVWQAGRFEIGLDKPKIMGIVNLTPDSFSDGGAYSQNAQTA
                     LVHAERLLKEGADILDIGGESTRPCADYVSPEEEWARVEPVLAEVAGWGVPVSLDTRR
                     TVVMEKALALGGIDIINDVAALTDEGALELLACQADTGICLMHMRGLPENMQINPKYQ
                     DVVGEVARYLKARAAECIAAGIAPQRITLDPGFGFGKTLQHNIALMRHLPELMAETGF
                     PLLIGVSRKRMIGELTGEADAAERVHGSVAAALASVARGAQIVRVHDVKATADALKVW
                     EALGINL"
     gene            complement(17132..18025)
                     /locus_tag="ACFM8S_000248"
     CDS             complement(17132..18025)
                     /locus_tag="ACFM8S_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFC6313385.1"
                     /translation="MIIVMSRRAAEADIAGVVAFIRSRGLREHISHGDERTVIGAIGD
                     DRVLSVREVQTLPEVEKAVRILDTWKTVSRENRAEDSRVAAKGVAFGGGETVRIAAEP
                     SVWRNADAVFLDPFFTSANLYDTSSADEGRGRCRRLAEQTASAHGAGKPVLVRVRNVC
                     HVEAALNAGADILYLGGGLMSDLAVLNEAGSLNIPLVLCKDKHHSAEDWLNAAEYVVS
                     RGNRHLILGESGVLSHTKDHPYRLDVESIVKVRQISHLPVIANITGLWNRDMPQEILY
                     GLAKAAGACGIVGTCFEKSGG"
     gene            18116..20230
                     /locus_tag="ACFM8S_000249"
     CDS             18116..20230
                     /locus_tag="ACFM8S_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFC6313386.1"
                     /translation="MDLLSVFHKYRLKYAVAVLTILLLAAVGLHASVYRTFTPENIRS
                     RLQQSIAHTHRKISFDADIQRRLLPRPTVILKNLTITEPGGDQTAVSVQETKIGLSWK
                     NLWSDQIQIEKWVVSSAELALTRDGKGVWNIQDLIDSQKRQASVNRIIVENSTVRLNF
                     LQEQLILKEINLNLQSPDSSGQPFESSGILVWGKLSVPWKSRGLFLSDGIGTPKISPF
                     HFEASTSLDGHGITISTTGTPAIRFNAGGADTAGLGLRADTSFRNLHLTAQIPALALR
                     NNSIKIETVNGAFTAGGEYAQWDGSFKLDKANLHSGIANIGNAEISGSFKTPRHQTNF
                     SLNSPLVWTENKGLDAPRLYVSTLQDTVNRLPQPRFISRLDGSLSVPNLQNWNAELNG
                     TFDRQTVAAKFRYTHEDAPHLEAAVALQKLNLTPYLDDVRQQNGKIFPDTLAKLSGDI
                     EAHLKIGKVQLPGLQLDDMETYLHADKGHIALSRFKSGLYGGHTEGGISIANTRPATY
                     RLQQNASNIQIQPLLQDLFGFHSFSGNGDAVIDLTAGGETRKELIRSLQGSLSLNISN
                     GAWHGIDMDNILKNGISGKTADNAAPSTPFHRFTLNSEISDGISRHIDTELFSDSLYV
                     TSNGYTNLDTQELSEDVLIRNAVHPKNKPIPLKITGTVDKPSITVDYGRLTGGINSRK
                     EKQKILEDTLLEQWQWLKPKEP"
     gene            complement(20294..21772)
                     /gene="ubiD"
                     /locus_tag="ACFM8S_000250"
     CDS             complement(20294..21772)
                     /gene="ubiD"
                     /locus_tag="ACFM8S_000250"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFC6313387.1"
                     /translation="MKYKDLRDFIAMLEQQGKLKRIAHPVSPHLEMTEIADRVLRAEG
                     SALLFENPIKPDGTRYGYPVLANLFGTPERVAMGMGADSVSKLREIGQTLAYLKEPEP
                     PKGIKDAFSKLPLLKDIWSMAPNVVKNAPCQEIVWEGEDVDLYQLPIQHCWPEDVAPL
                     VTWGLTVTRGPHKKRQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEFRKLNPDTPYP
                     VAVVLGCDPATILGAVTPVPDTLSEYQFAGLLRGSRTELVKCIGNDLQVPARAEIVLE
                     GVIHPNETALEGPYGDHTGYYNEQDYFPVFTVERITMRENPIYHSTYTGKPPDEPAVL
                     GVALNEVFVPLLQKQFPEITDFYLPPEGCSYRMAVVSMKKQYAGHAKRVMMGCWSFLR
                     QFMYTKFIIVVDDDVDVRDWKEVIWAVTTRMDPVRDTVLVENTPIDYLDFASPVSGLG
                     GKMGLDATNKWPGETDREWGSVIKKDPAVTAKIDGIWEELGL"
     gene            complement(21792..22307)
                     /locus_tag="ACFM8S_000251"
     CDS             complement(21792..22307)
                     /locus_tag="ACFM8S_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HFC6313388.1"
                     /translation="MYTCTSCGEKHEDMPAIGFTAPDPYNQLSEEERIAYQAECDSDF
                     CIIRYPDQTDRFIRAVLPIPIIGHQETLEYGVWVSVSEKSFNDYQSHFHDNPENVVYF
                     GMICNRLPPYESDTFGLHCNVVTQPDGQRPLLQLHQSGGHPLVRDFYHGIEYAEAQAR
                     IGAVSESDCSG"
     gene            complement(22314..22748)
                     /locus_tag="ACFM8S_000252"
     CDS             complement(22314..22748)
                     /locus_tag="ACFM8S_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313389.1"
                     /translation="MCGLWLLRALTAKLPQQRYAALFITLFFAAVLVFRRHDSMYWYP
                     VLVNLMMLAVFGGSLFAGQTVIEKLARLQQPDLPEKAVRYTRRVTQVWCVFFIINGTL
                     TALLARLGRYDWWAVYTGVIAYVLMGMLFAGEWLYRKLVLKV"
     gene            complement(23029..23112)
                     /locus_tag="ACFM8S_000253"
                     /pseudo
     CDS             complement(23029..23112)
                     /locus_tag="ACFM8S_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
     gene            complement(23115..23615)
                     /locus_tag="ACFM8S_000254"
                     /pseudo
     CDS             complement(23115..23615)
                     /locus_tag="ACFM8S_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714696.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
     gene            23671..24235
                     /locus_tag="ACFM8S_000255"
                     /pseudo
     CDS             23671..24235
                     /locus_tag="ACFM8S_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611451.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            24239..25348
                     /locus_tag="ACFM8S_000256"
     CDS             24239..25348
                     /locus_tag="ACFM8S_000256"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HFC6313390.1"
                     /translation="MEAQLGAPIEDYAPPKHWTRKQLRSMGRVSYLCVDAAEQALTDA
                     GLLGDESITDGRMGVACGSSSGSTKDIGDVGELLLTGMSRNFSANTYVRMMPHTAAAN
                     IGIFFGLKGRIIPTSSACSSGSQGIGYAYEAIKYGLTDMMLAGGGEEFFPSEVYVFDS
                     LYAASRRNGEPEKTPRPYDANRDGLVIGEGAGIFVLEELEHAKRRGAIIYAELVGYGA
                     NSDAYHISTPRPDAQGAILAFQTALQHAGLAPEDIGWINLHGTGTHHNDSMESRAVAA
                     VFGNNTPCTSTKPQTGHTLGAAGAIEAAFAWGIADRQSNPEGKLPPQLWDGQNDPNLP
                     AINLTGSGSRWETEKRITASSSFAFGGSNCVLIIG"
     gene            25402..25477
                     /locus_tag="ACFM8S_000257"
     tRNA            25402..25477
                     /locus_tag="ACFM8S_000257"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25435..25437,aa:Ile,seq:cat)
     gene            25552..26310
                     /locus_tag="ACFM8S_000258"
     CDS             25552..26310
                     /locus_tag="ACFM8S_000258"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC6313391.1"
                     /translation="MKKVSVLIVAKNEANHIQECIESCRFDKEVIVIDDYSTDNTAEI
                     AEGLGAKVFRRHLNGDFGAQKTFAIEQAGGEWVFLIDADERCTPELSDEISKIVQTGD
                     YAAYFVERRNLFPNHPATHGAMRPDSVCRLMPKKDSSVQGKVHETVQTPYPKRRLKHF
                     MYHYTYDNWEQYFNKFNKYTSISAEKYREQGKPVRFVRDIILRPIWGFFKIYILNKGF
                     LDGKMGWIMSVNHSYYTMIKYVKLYYLYKSGGKF"
     gene            26311..27375
                     /locus_tag="ACFM8S_000259"
     CDS             26311..27375
                     /locus_tag="ACFM8S_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFC6313392.1"
                     /translation="MEKEFRILNIVSAKIWGGGEQYVYDVSKALGLRGCTMFTAVNKN
                     NELMHRRFSEVSSVFTTRLHTPNGLFSLYALTRFIRKNRISHLMIHTGKIAALSILLK
                     KLTGVRLIFVKHNVVANKTDFYHRLIQKNTDRFICVSRLVYDVQTADNPFKEKYRIVH
                     NGIDTGRFPPSQEKPDSRFFTVAYAGRISPEKGLENLIEACVILHRKYPQIRLKLAGD
                     GHPDYMCRLKRDVSASGAEPFVSFEGFTEKLASFYRQSDVVVLPSLVPEAFGLSLCEA
                     MYCRTAVISNTLGAQKEIIEHHQSGILLDRLTPESLADEIERLVLNPETKNALATAGH
                     QCVANRFTINHTADKLLDAI"
BASE COUNT         6396 a         7801 c         7164 g         6227 t
ORIGIN      
        1 tcccataaaa aaaacggagc agatacctgc cccgttttta tttaatccga aattttaatc
       61 taaatttaga attttgcacc ggattggttt gccatatagt caacagccgc tttgacttcg
      121 tcatcgctca aacctgcatt gccgcctttg gcaggcatcg cgttaaagcc ttcaagggcg
      181 tgtttgtgca aggtttcttt gccttttttg atacgcggtg cccaatcgtc ttttttgcct
      241 atgccgggaa taccgggaat cgaaccgccg tggcacacct gacaggttgc ttcgaagact
      301 tttttaccgt caacgccgac cgcaggggct gccgcaccct tgtcttctgc cttcgcttct
      361 gccggagctg cactatcggc aggagcagaa gctgttcctg aagcggcatt gtcggcaggc
      421 gcagcctcat caggattcgg gaaagaaccg ccgcttttgt tcgccatgta agtaatcgcc
      481 cgtttgagtt cctgatcggt caggtctgcc gcaccgcctt tggcaggcat ggcgttaaag
      541 ccgttcagcg cgtgttggaa caaggtatcg aagccttgcg cgatacgcgg tgcccaatcg
      601 ccgttgtgtt ccagtttcgg agcgttcggc acattgctgt ccgccgcgtg gcattggata
      661 cagattttgc cgaaaatctg ttcgccttgg cgttcgccga cggggatgcc gtcgcccatc
      721 gtcaattgtc cgacaggctg gatacgggtc tgcgttgctg cttccgtagt ggcatcgaca
      781 tcgccgaacg agccgctgcc cgccagctta atcaggaaat aaaggactgc aataacaata
      841 acgataccgc tcacaagggt aaacagtgca gagccttggg ctttgttgtc gcggagttgt
      901 ttcatttggt aggcctcgcc gtcaggttag gttgtgctgt aaattatagt ttggtgtgtt
      961 aaacgcagtt aacaatattt tgctggatta tactgaattc acagggtctt tccaatcgct
     1021 atcattgaaa atatgaaaaa atttgccaac ggtatctgta tacaacaaat aatcctttga
     1081 aaataatggt ttatcttcaa gaaaactctc cttatgccgc catacgccgc ctgccggcgc
     1141 aagataacct ttgccaattt gcagaattta cgttaacctt acgttttacg cacccatagc
     1201 tcagttggaa gagtgtcagt ttccgaagct ggaggtcaca ggttcgatcc ctgttgggtg
     1261 cgccaattat aaagagaccg tctgaaagat aaatattttt cagacggtct tttgacttac
     1321 ttcaaactct tatttcaaga cttccgcaaa tgcgcgggca acatagtcgg tattcgacgt
     1381 attcagtccg gcgacgcaca tcctgccgga atccagcagg taaacggcaa attcgtcgcg
     1441 cagcctgcgg acttgttcca cgctcaatcc tgtgtagccg aacattccgc gctgtttgat
     1501 gaaataagtg aaatcgcgat cggggatttg cgcagttaag acatcataaa gtttctgccg
     1561 catggcgcgg atgcggtcgc gcatcatata aacttcgttt tgccacaaga cgtaaagttc
     1621 ggggctgttc atcacgtcgg cggcgatata cgcgccgtgc gcgggcgggc tggagtagat
     1681 gcggcggaca gtgaatttga gctgtccgaa caccaaatcc gcttcttcct tattcgggca
     1741 aaccacgctt aagccgccga cgcgctcgcc gtagagcgac aggtttttcg agaaggaatt
     1801 gctgacgaac aagggcaatt ccatttccac cgctttgcgg acggcgtagg catcgctgtc
     1861 caaatcgccg ccgaatcctt ggtaggcaat gtccataaac ggaatcagtt tgcgcctttt
     1921 gatgatgtgc aacacttcat cccattgctg ttccgacata tccacgccgg tcgggttgtg
     1981 gcagcagggg tgcagaatga ggacgctgtt ttcgggcagg gtgttgaaaa atgcagtcat
     2041 ttcgttgaat ttcacgccga cagtggcggg gtcgtaatat gggtaagtgc cgacctcgaa
     2101 acctgcacct tcaaaaatgc cgcgatggtt gtcccaagtc gggtcgctga cgtaggcgcg
     2161 cgcttcggga aaccagcggt gcagaaagtc cgccccgact ttgagcgcgc ccgatccgcc
     2221 cagagtctgt acggtaacga tgcgcccttg cgcgagcgcg gggttgtctt tgccgaacaa
     2281 taaatgctgc accgcgctgc ggtaagcatc caaaccttcc atcggcaggt agggcgacgg
     2341 cgcaggcgcg gcggcacggg cagtttcggc gcggctcacg gattccaaca cgggcatttt
     2401 gccttcgtcg tcgaaataaa tgcctatgct caaattgact ttttcggggc gcgggtcgtt
     2461 tttgaaggtt tcgaccaaac tcaaaatcgg gtcgccggga tagtattcga tgtgtcggta
     2521 catagtcctt accttttact ttttcaaagg attttctttt tcaacaatac accactttcg
     2581 atatggtgcg taaacgggaa ttggtcgaac aaggcggcac gttcgaccgc atgggtttcc
     2641 gccaaggtgt ccaaattggt acgcagcgtt tcgggattgc aggaaatgta gatgatgttg
     2701 tcaaactgcg acaccagctt caaagtttcc tcatcgatac cggcacgcgg cggatcgacg
     2761 aaaatagtgg aaaatgcgta atccgtcaaa gcaataccgc catccttaag gcgtttaaac
     2821 tcacgttttc cggtataggc ttcggtaaat tcttcagcag acagacgggc gattttgatg
     2881 ttgccgatgc ggttggcttc gatattccat tgcgccgcgc tgacggaggt tttggagatt
     2941 tcggttgcca aaacacgttc aaacttttca gataaaggaa gcgtaaagtt gccgtttccg
     3001 caatacagtt cgagcaaatc cccatccaat ccttctgctg caccgcaggc ccattcaagc
     3061 attttctgac acacggcggc attcggttgg gtaaaactgc cttcaatttg ccgataacgg
     3121 aaatcccggt tgccgacctt caaagtttcc gttacatagt cctgttttaa gactattttc
     3181 tgtcccctgc tccgcccgat gacggaaata tccaactgtt gctgtaacgc ttgcgccgcc
     3241 tgcatccact cattatcaag ccttttgtgg taaatcatgg taaccagcat ttccccgctg
     3301 agcgtagaca aaaattcgac ggcataccaa cggtttctca gttcggcaga ttgggcggca
     3361 acggcaatca attctggcat caggcggttg acggcttcgg aagccgcttc aaaacggtcg
     3421 caacggatca ggcttgcccc gccagccttt tgtccttttt caaacatggc ataaaacatt
     3481 tcaccgcctt cgtgccaaat acggaactcg gcacgcatac ggtaatgttt gtccggagat
     3541 tcgtacactt cccactcagg aacatccaaa cctgcaaaaa gggttttaag gtggtctttt
     3601 ttatcttgaa gctgctgcgt gtagtcgctc atatcgctat cctgaaaaat cagaccggat
     3661 tataagccga ttcactgcaa aacacgcaac ccctttaccg gcatataacc cgttttcctc
     3721 caacttttat gctaaaatcc gccgtctcca acataccgcc gacagaagga aaatatggca
     3781 ggcaacactt tcggacaact cttcaccgtg accacgttcg gcgaaagcca cggcgcaggt
     3841 ttgggctgca tcatcgacgg ctgcccgccc ggactggcat taaccgaatc agacatccaa
     3901 gccgacctcg accggcgcaa acccggcacc agccgccacg ttacccaacg ccgcgaagcc
     3961 gaccaaatcg aaatcctctc cggcgtattc gaaggcaaaa ccaccggcac gcccatcgcc
     4021 ctcttaatcc gcaataccga ccagcgcagc aaagactacg gcaacatcgc ccaaagtttc
     4081 cgccccggcc atgccgacta tacctattgg cacaaatacg gcacgcgcga ctaccgcggc
     4141 ggcggcagga gctccgcccg tgaaaccgcc gcccgcgttg ctgccggagc cgttgccaaa
     4201 aaatggttga aagaaaaatt cggcacggaa atcaccgcct acgttaccca agtcggcgaa
     4261 aaagaaatcc ggtttgaagg ctgcgaacac atttcccaaa atcctttttt tgccgccaac
     4321 catagccaaa ttgccgagct ggaaaactat atggacagcg tgcgcaaatc cttggattcc
     4381 gtcggcgcga agctgcatat tgaagccgcc aacgtccccg tcggcttggg cgaacccgta
     4441 ttcgaccgcc tcgatgccga aatcgcctac gccatgatgg gcatcaacgc cgtcaaaggc
     4501 gtggaaatcg gcgcaggttt tgacagcgta acgcaacgcg gcagcgagca tggcgacgag
     4561 ctcacgcctc aaggcttcct atccaaccac tcaggcggca tcttaggcgg catcagcacc
     4621 gggcaagaca tctgcgtcaa tatcgccatc aaacccacca gctccatcgc caccccgcgc
     4681 cgcagtatcg acatcaacgg caaccccatc gaactcgcca cgcacggcag gcacgacccc
     4741 tgcgtcggct tgcgcgccgc gccgattgcc gaagctatgc tcgccttggt tttaatcgac
     4801 cacgccctgc gccatcgtgc gcaaaatgcg gatgttgccg tggatacgcc cgatatcgcc
     4861 cgctcggaca agtaaaaaga tttagccaaa acacgggctt tatactaaaa taccgaccat
     4921 tgccgtgccg tctgaacgca cgattcttag aggaatacaa ccgaaatgac acaagaaacc
     4981 gctttgggtg cggcactgaa atccgccgtc caaactatga gcaaaaagaa acagaccgaa
     5041 atgatcgccg accatatcta cggcaaatac gatgtattca aacgcttcaa accgttggcg
     5101 ctcggcatcg atcaggattt gattgccgct ttgccgcaat acgattccgc actgattgca
     5161 cgcgtcctcg ccaaccactg ccgacgtccg cgctatctga aagccttggc gcgcggcggc
     5221 aaacgtttcg atttgaacaa ccgtttcaaa ggcgaagtta cccccgaaga acaggcgatc
     5281 gcgcaaaacc atcctttcgt gcagcaggct ttgcaacagc agtccgccca agctgccgcc
     5341 gaaacgccgt ctgttgaagc cgaagcagcc gaatcttccg cagcagaata aatccccaaa
     5401 tgaaatgccg tctgaaaacc gatttggttt cagacggcat ttttccgtat gcggcaatca
     5461 cggttcaaat atcgagttcc gccgtatcgc cttcgcgttc catccaagcg cggcgggcgg
     5521 cggcttcgcc tttgcccatc agtttgacga agatgtcgcg cgtctcgtca tctgcacctt
     5581 ctgggatttg tacctgcaac aggcggcggg tgtcagggtg catggtggtg tctttgagct
     5641 ggtcggggtt catttcgccc aagcccttga aacggctgat ggaataggcg gtttctttga
     5701 cgccttcttt ttgcagccgc tccaaaatgc tgtcgagttc gttttggtcg agggcgtaga
     5761 atttgcgggc aggtttgctt ttgccttgtg cgttgacatc gacgcggaac agcggcggct
     5821 gggcgacgta gatgtgtccg tcggcaacca gtttcgggaa gtggcggtag aataaggtca
     5881 gcagcaaaac ttgaatatgc gagccgtcca cgtcggcatc ggacaggatg gcgattttgc
     5941 cgtagcgcag gccgcttaaa tcgggatggt cgttaatacc gtgcggatcg acgccgatgg
     6001 cgacggaaat gtcgtggatt tcggcgttgc cgaagagttg gtcggggtgg acttcaaagc
     6061 tgttgagcac tttgccgcgc aggggcagga tggcttgggt ggctttgtcg cgggcgagtt
     6121 tggctgagcc gccggcggaa tcgccttcga cgaggaagag ttcgttttcg cggatgtctt
     6181 cgctttcgca gtcggtcagc ttaccgggca ggacggcgac gccgctgcct tttttctttt
     6241 caatcttttt aaccgaacgc atccgcgcct gtgcctgacg gatggcgagt tcggcgattt
     6301 ttttgccgaa gtccacgttt tggttcagcc acagttccaa agggtcgccc gatacggtag
     6361 caacgagttt cagcgcgtcg cggttggtca gtttgtcttt ggtctgacct tggaactgcg
     6421 ggtcgaggac acgggcagag aggacaaagg cagttttgcc gaacacgtcg tcgctttgca
     6481 ctttcacgcc gcgcggtaag aggttgtgca ggttgatgaa gttgctgacg gcgttgaaca
     6541 cggcttgttt caagcccgct tcgtgcgtgc cgcccagcgg ggtggggatg aggttgacat
     6601 agctttcgtt ggcacacgag ccttcttcca gccaagtcag ggcaaatgct gcgccttcgc
     6661 cgatgctgaa atcgccgttg tgttcgtctg aaatgtagtt ttcgcaagag aacagcggta
     6721 cggcttcctg cgcgtcggca atcaggtcgg tcagatagct tttcaggccg tcggggtaat
     6781 gccaggtttg ggtgtgcgct tcgtcttcgc ctttgaccgg acgggtcagg gaaacgcgca
     6841 cacccggcag cagcacggct ttggcacgca gcaggcgttc gagttcggga atgctgtaat
     6901 tcgggctttc aaaatatttg ccgtccggcc acacgcgcac ttcggtgccg ctgtctttga
     6961 cggcgcattt gcccacttgt gccaacggtt cgaccacgtc gccgccggca aacacgatac
     7021 ggtggatttt gccttcgcgt ttgaccgtta cctcaaggcg ggtggaaagg gcgttggtca
     7081 cggatacgcc cacgccgtgc aggccgcctg aaaaggcata cgcgctgccg ccgtcttttt
     7141 tgttgaactt gccgcctgcg tgcagacggg tgaacacgag ttcgactacg ggtacgcctt
     7201 cttcagggtg cagaccgacg ggaataccgc ggccgttgtc gcgtacggaa agcgaaccgt
     7261 cttcatgaat ttgcacgtcg attgccgtag cgaaaccgcc caacgcctcg tccgccgcgt
     7321 tgtcgatgac ttcttggcag atatgggtcg ggctgtcggt gcgggtgtac ataccgggac
     7381 gttctttgac cggttccaaa cctttgagga cggtgatgct ggattcgctg tattggttgt
     7441 ttttagccat aggaataatc tgaaagtaag aaaaacaacg ctttcagacg gcctgaaagc
     7501 gttgcgttcc gttgttttac cggttgccgg aaagttggcg ggcggcaaag tcttcataac
     7561 tttccatacc gcgcaggaag cgggaagaaa gttctttcaa tgcccccaaa taaacgtcgc
     7621 gtttgaaatc aatcacttcg tcaacgggcg cccaatattg gtgccaacgc cagccgtcaa
     7681 attcggggtg gcgggtggcg cgcaggttga catcgcaatc ccggccggtc aggcgcagga
     7741 gataccaaat ctgcttctgt ccgcgataag agccgcgcca ttcgcggcgt acccagttgt
     7801 tcggcacgtc gtaacgcagc cagtcgcgcg tccgcccgac gattttgacg tgttgcggca
     7861 gaagtccgac ttcttcgtaa agctcgcggt acatcgcggt ttcggggctt tcgcccggct
     7921 tgatgccgcc ttgcggaaac tgccaagaat gttcgcgcac gcgcttgccc caaaagactt
     7981 cgttacgttc gttaatcagg ataataccga cattggggcg atagccttcc ctgtccaaca
     8041 cggtgtcgcc ctccgttaaa ttcaatcgta cgattttccc acaaatcggg cggttttgac
     8101 aaatcagacg gcatggttca agcatgtcga aacacagacg gactcgggaa aatatcttaa
     8161 atttggttta caataatgtg tttcaaatta ttcgggaatc agaccatgtt agatatccaa
     8221 ttgctccgca gcaacaccgc cgctgtggcg gaacgcttgg cggcgcgcgg ttacgagttt
     8281 gatgccgcgc gtttcaatgc gctggaagaa caacgcaaag ccgtccaagt gaaaaccgaa
     8341 gaactacaag cctcgcgtaa cagcatttct aaacaaatcg gcgcactgaa aggtcagggc
     8401 aaacacgaag aggcgcaggc ggccatggat caagtcgccc aaatcaaaac cgatttggaa
     8461 caggctgccg ccgatttgga tgccgttcaa aaagaattgg acgcatggtt gttgagcata
     8521 cctaacctgc cgcacgaaag cgtacctgcc ggtaaagacg aaaccgaaaa cgtcgaagtc
     8581 cgcaaagtcg gcaccccgcg cgaatttgac tttgaaatca aagaccatgt cgatttgggc
     8641 gaacctttgg gtttggattt tgaaggcggt gcaaaactct ccggcgcacg atttaccgtg
     8701 atgcgcggac aaatcgcccg tctgcaccgc gccttggcac agttcatgct ggatacgcac
     8761 acgctgcaac acggctacac cgagcattac acgccttata tcgttgacga tacgacgctg
     8821 caaggtacgg gccaactacc aaaatttgcg gaagatctgt tccacgttac ccgtggcggc
     8881 gacgaaacca aaaccaccca atacctgatt ccgacagccg aagttaccct gaccaatacc
     8941 gttgccgaca gcattatccc gtccgaacaa ctgccgctga agctgaccgc gcattcgccc
     9001 tgtttccgca gcgaggcggg ttcgtacggc aaagacacgc gcggtctgat tcgccagcac
     9061 cagttcgaca aagtggaaat ggttcaaatc gttcatcccg aaaaatcata cgaaacgctg
     9121 gaagaaatgg tcggccatgc cgaaaacatc ctgaaggctt tggaactgcc ctaccgcgtg
     9181 attaccctgt gtaccggcga catgggcttc ggcgcggcaa aaacgtatga cttggaagtt
     9241 tgggtgccgg cgcaaaatac ctaccgcgaa atctcaagct gctccaactg cgaagatttc
     9301 caagcccgcc gcctgaaggc gcgtttcaaa gacgaaaacg gcaaaaaccg cttggtacat
     9361 actttgaacg gctccggttt ggcagtcggc agaaccttgg tcgccgtgtt ggaaaaccat
     9421 caaaacgccg acggcagcat caacatccct gccgcactgc aaccgtatat gggcggcgtt
     9481 gccaagttgg aagtgaaata agccttaacc ctgaacataa atgccgtctg aaacctggtt
     9541 cagacggcat ttcctttaaa cttttaaaac acatcaaccg tcggcacgaa ccgcattgcc
     9601 gcaatcgccg gtctgtccga cctcgcggat attggacagc gtaacttccg aaatattacc
     9661 caacgcctct tccgtcaaaa atgcctgatg gccggtaaac agcacattat gacaagacga
     9721 caggcggcgg aacacgtcgt cggtaatcac atcgttggat ttgtcttcaa aaaacaattc
     9781 gcgttcgttc tcgtacacgt ccatacccaa tgcgccgatt ttccggcgtt tcaacgcctc
     9841 aatcgcggca gaactgtcaa tcagaccgcc acggctggtg ttgataatca tcacgccgtc
     9901 tttcattttg tcgaacgccg cttcgttcag catatagtgg ttttccggcg tggaggggca
     9961 atgcagcgtg atgatgtccg actgggcata cagctcgtct aaatccacat atttgccgcc
    10021 gattttttcc gcttcggggt tgcaaaacgg atcgtaagcc agcaggttca tgccgaaacc
    10081 ctttaaaatc cgcatggttg caataccgat tttccccgtg ccgataacgc ccgccgtttt
    10141 gccgtacata ttgaaaccgg tcagaccttc cagcgaaaaa ttcgcatcgc gggtacgctg
    10201 ataggctttg tggatacggc ggttcaacgt cagcatcaga ccgaccgtat gttccgcaac
    10261 cgattcgggc gaataggcag gcacgcgcac gactttcaag cccaactctt cagccgcttt
    10321 caaatccaca ttattgaagc cggcacaacg caacgccaca gttttcacgc caatttgcgc
    10381 caatttttcc aacacgggcc ggctgccgtc gtcgtttaca aaaatacaga ccgcctccgc
    10441 accttccgcc attttcgccg ttttcacatc cagcataaaa tcaaaaaact ccagctcgaa
    10501 gccgaaatgc cggttggcgc gggtaaaatg ttcgcggtca taacttttcg taccgtaaat
    10561 cgcaatcttc atcaatatgt ccagttgtcg cctatggttg agaaacggca taatacactg
    10621 aattcaaaca aatcagtaga atatggcaga ttaaaattga ttgcatgcac ggcatttcca
    10681 tttcaaaaca caaaactcaa tcgcccattg ccgccaaaag ctccgcctga tgttcggcaa
    10741 tcagggcatc ggtgatttct gccaaatcgc cgtccatcac aaaatccagc ttgtgcaggg
    10801 tgaggttgat gcggtggtct gttaccctgc cttggggata gttgtaggtg cggatgcgtt
    10861 cgctgcggtc gccgctgccg atgagggatt tgcgttcggc ggcttctttg gcttgggctt
    10921 cgcgtttttg cgcgtcgttc aggcgggcgg cgagaacttt cattgcctgc gctttgttgg
    10981 cgtgttggct gcggccgtct tggcattcga ccaccatgcc ggtgggcagg tgggtaatgc
    11041 ggacggcgga atcggttttg ttgatgtgct gaccgcccgc gccggatgcg cgaaaggtgt
    11101 cgatgcgaag gtcgacgggg ttcaactcga tgtcttccag ttcgtccgct tcgggcatga
    11161 cggcaacggt gcaggcggag gtgtggatgc ggccttggct ttcggtggcg gggacgcgct
    11221 gcacgcggtg tccgcccgat tcaaatttca aacggctgta cgcgcctaag ccgataatac
    11281 gggcgataac ttctttatag ccgcccaact cgctttcgtt ggcggacacg atttcaacct
    11341 gccagcggtt gcgttcggcg tagcggctgt acatacgcag caaatcgccg gcaaacagcg
    11401 cggcttcgtc gccgccggta ccggcgcgta cttcgataaa gatgtttttg tcgtcgtcgg
    11461 catctttggg cagcagcaat ttttgcagtt cggtatcgag cgcaccgatt ttggctttcg
    11521 ccgcttcgat ttcttcggcg gcaaagtctt tcatttcggg gtcggacaac atttcttcgg
    11581 catccgccaa gtcgctttgg gcaagccgat agttttggaa cacttcgacg acgggggtca
    11641 gttcagcgtg ttcgcgcgtg agcttgcggt agttgtccat atcggacgtg gcttcgggtt
    11701 gccctaaaag gtgggtaact tcttccaacc ggtcgccgag ttgttgcagt ttttctaaga
    11761 tagacggctt cataattctt ccataacaaa cgccgcctga atgttcagac ggcatcaaaa
    11821 cgcggtcatt ataatatatt ttctgaatat tcaaaaagat aatctaggga cggagcaggc
    11881 tgtcggcatt gcgttccgtt atcgcggcga tttccgccgc gccggtaccg cgtatctgcg
    11941 cggcaatttc ggcaatccga agaatgttgg caggcgtatt gacggtgttt ttcaacataa
    12001 acgggctatc cgtttccaat acgaaatccc cgtcgttcaa ggctttaagc gtatcgcgca
    12061 ctttacgcgc gttcggattg agcaacaacg aaccgatgcc gattttgaaa cccagtttcg
    12121 tcaacacgcg cgcttcttcc gcgctgcctg agaaggcgtg aacgatgccg ccttgggcaa
    12181 agcctgtctg tttgacagcg gcggcgatgt cggcggtggc tttgagatta tggataatca
    12241 cgcggcggcg cagggtttgc gcgatggcaa gctggcggac gaaaacttgg atttgccgtt
    12301 cgcgctgcgg cggcgtttgg gttttgtcgt aaaaatccaa accgatttcg ccgacccatg
    12361 cctgcggata acgtgccaac atcgcttcca ggcggacgca atcacgctcg gcaatgccgt
    12421 ctgaaaacca aggatggatg ccgagcgcaa tacggatttg accgtgttcg gacggcattt
    12481 ccgacagttc cgccacgctt tgccaatcct gcggacaggt ggcgggaacg ataaaccgct
    12541 tcaccccaac tttccgcgcg gcggtcagga tgtgcggcag gttttcgcgc agggcggcat
    12601 cggcaaggtg gcagtgggtg tcggtgaagt tcatttcgat tccgcaatgc ggcaggttca
    12661 cataaaaata aggcagggcg gcaagggatg ccgtgtacgg gcggcgcata aggcgcggtc
    12721 aacgccccgt cattgccatt tcaatccgct ataacttcag tcttgccaac tctttttcca
    12781 cctcttcctt accccagcct tgcgatacgg cgagggtcat aagcgcggtg gcggtttcga
    12841 ggttgcattt gccgccgttg atgatgcctg agttgcggaa cgcgttgcct tgcgcgtaaa
    12901 cggcagcggt tctgccttgt cggacttggc tgatgttgag cagcagtttg ccttgctgcg
    12961 taaagtcttg cacggcgcgg atgaaacctt cgtctgcggg cgtgttgccg tgtccgtagc
    13021 tttgcaggat aagggcttgg gcgggaagct gtccgagtcc gtcggcaagt tcttggacgg
    13081 caaagccggg gataagcgtg cggacggcga tttttgcctg cgggtcggga taacggattt
    13141 tgaggccgtc tgaaacatct gccgcgtcct gattcgggat gcggacattg tgccagccct
    13201 gggtttcgtc ccactccgcc agtgcgccga aatgcggatt gtcgaaaccg gcggcggttt
    13261 cggtgctgac tttgctgctg ccgacggcgg gataaagttt gccgtcaaag gcaataagcg
    13321 tctgcttcaa tccgagcggg aatgcggcaa cggcagtcgc cagattgcgc ggcgcgtcgc
    13381 tgcccacggc gttgtacggc cattgggaac cggtcaggac aatcggtttg cccaaaccct
    13441 gcagcgcgag ggcgaacaga ttggcggtat aggcgagcgt gtccgtaccg tgcagcacca
    13501 aaacgccgtc gtactgcggc agcgcggcgg caatgatgtc cagccaagcg caccagtttt
    13561 gcagttggac ggcggaagaa tcaatcagcg tcccacaaac gtgccaatcg aaatccatgc
    13621 cgtttgaaaa cggcgcgagt gcctgcccga ccagcgcggt atcggggcgc aggccttcgc
    13681 tgctttgcgt catgccgatg gtgccgccgg tataaaggac aaaaattttc tgtttcatgg
    13741 ctcatcctga aaatgcaaag accgcctgaa acgcgaaatg cgtccggacg gtcgaaaatc
    13801 tgatttactg ctgcttctgt gccgcttttt tcgctgcctt ttccgccttg cgtttggcgc
    13861 gtttttcgct caattgtgcg cggtaaagct gataatgtcg gcgtttgcgc caccagaacc
    13921 aagccagcgc cgccgccagc acgcccaatg cgatgaagat gccggattgc aggctgtgca
    13981 ttttcgccat cagccaatcg atgttgtgcg cgccgtactc gcccaagtaa atccaaacgg
    14041 gcacggaaat cagcgcggca agcccgtcca taatcagaaa gcgcagatac gatactttgc
    14101 ggctgatgcc ggcggtaacg aaaacggcag tccgcaaacc cggcaggaag cgggcgacaa
    14161 acagaaccca gttgccgtat ttgtcgaatt tttcctgaac ctgtgcgtaa cgtttcggcg
    14221 tcatgatgcg cgcaatcggt ttgaacttga ggattttctg cccccagatg cgtccggcgg
    14281 cgaacatgat gccgtccccg accaatacgc cgagcatacc gactgcaaac ataatatgcg
    14341 gattggtata acccataccc gaaatcacgc cgcctgttac caaggtcaaa tcctcgggaa
    14401 tcggcacgcc gaaaccgcag atgaccaaaa cgaaaaacac ggccgcgtag ccgtattcga
    14461 caaaaaaggc ttccaaaagg gcaaacatag cggatattcc attgtcggag ataaaaagtc
    14521 agaacaagcc gaaacatttt ctgcgcgaaa cggcattcta tcaaagatta tgccgtctga
    14581 aagcggcagg gggcagattc gcgccctctg ccggacgttc agacggcatt taaacaggaa
    14641 aaccgccgct gttgaaacgg ttttgcctga ttttgcctaa atgccgccga tggcggcggc
    14701 aatgcgttcc gccccttcgc gcgcccaatc cgcctgccgc gcctccacca tcacgcgcac
    14761 gacgggttcg gttcccgaag cgcgcaacac gacacgccct ttgccttcga gttctttttc
    14821 cacttccgcc aacacgtctt ttgaggcttc ctgccagttt tgccctttgc cgatacggac
    14881 attaatcatc gtttgcggat acggctgcca gtcggcgcaa acggcggcaa ggtcttggtt
    14941 cagcgtttgc aatgccgcca aaacctgcaa ggcggaaata atgccgtcgc cggtgttgtg
    15001 tttgtccatg cacaaaatat ggccgctggc ttcgccgccg acgagccagc cgcgttggtt
    15061 cagctgttcc aacacatagc ggtcgccgac tttggcgcgg cagaaatcca cgccctgctc
    15121 tttcagggcg atttccatcg ccatattggt catgaccgtg ccgaccacgc cgccgatgtt
    15181 gataccttct cgggcgcggg ctttagcaat gacgtaaatc aggctgtcgc cgtcgtaaac
    15241 cttaccgttt ttatcgacca tcatcaggcg gtcgccgtcg ccgtctaagg cgatgccgta
    15301 gttggcttca tgctgtaaaa cggctgcctg gagtgtcttg gtataagtcg caccgcattt
    15361 ttcgttgatg ttgtagccgt tgggttcgtc gccgatgctg acgacttgtg cgcccagttc
    15421 gtgaaacact ttgggtgcga cgctgtaagc cgcgccgttg gcggcatcga cgaccagctt
    15481 cagcccgcgc aaatcggaat gcgtagggaa ggtggatttg caaaattcga tatagcggtc
    15541 gtccgcgccg ttgatgcggc gtgcgcgacc gagacgggcg gacggttggg ttttcatttc
    15601 gccgtcgatt ttggcttcga tttccaactc gacttcatcg gaaagtttca cgccgccttc
    15661 ggcgaagaat ttgatgccgt tgtcggaata ggcgttgtgc gacgcggaaa tcatcacgcc
    15721 ggcggacagg cgcaacgcgc gggtcagata agccacgccg ggcgtgggca gcggtccggt
    15781 ctgtaccaca ttcacacccg ccgccgtaaa accggccacc aaagcggctt ccagcatata
    15841 gccggaaatg cgcgtgtctt tgccgatgag gacggtcggt ttctggtcgg tgtcgtgctg
    15901 caccagtacc tgccccgccg catagccgag tttcaataca aaatcgggcg taatcggaaa
    15961 ctgcccgact tcgccgcgca cgccgtccgt gccgaaatat ttttttgcca tatgttgctc
    16021 cgagaatgtg aaccgttgtc cgagattata cagtcagttt gtgccttgct gtctgcaccg
    16081 ttgatgccgt ctgaagccgc cccgcccttt tcagacggca tgaagtatgc gaaccgccgt
    16141 ttacagattg atgcccaacg cttcccacac cttcaatgca tccgccgtcg ccttcacatc
    16201 atgcacccgc acgatttgcg cgccgcgcgc cacggaagcc agcgccgccg ccacgctgcc
    16261 gtgtacgcgt tccgccgcgt ctgcctcgcc agtcagctcg cctatcatac gtttgcgcga
    16321 cacgccgatg agcagcggaa aacccgtttc cgccatcaat tcgggtaaat gccgcatcag
    16381 tgcgatattg tgttgcaagg ttttgccgaa gccgaaaccg gggtcgagtg taatgcgttg
    16441 cggggcgatg cctgccgcga tgcattccgc cgcccgcgct ttcaaatacc gcgccacttc
    16501 accgacaaca tcctgatatt tcggattaat ctgcatattc tcaggcaagc cccgcatgtg
    16561 catcaggcaa atgcccgtat ccgcctgaca cgccagcaat tcgagcgcgc cttcgtcggt
    16621 caacgccgcc acatcgttaa taatatcgat gccgccgagt gccaacgctt tttccataac
    16681 caccgtgcgg cgcgtgtcca aactgacggg aacgccccac cccgccactt ctgccaatac
    16741 aggctcaacc cgcgcccatt cttcttcagg cgaaacataa tccgcgcacg gccgcgtcga
    16801 ttcgccgccg atgtcgagaa tgtccgcacc ctctttcaaa agccgttcgg catgcaccaa
    16861 agcggtttgg gcattttgcg aatacgcgcc gccgtcggaa aaagaatcgg gcgtgagatt
    16921 cacgataccc atgattttcg gtttgtccaa accgatttca aaccgtcctg cctgccaaac
    16981 gtgtcgtgcc atctgaactc ctcccaaaat aaaaaacaga ttatatgccg tctgaaaccg
    17041 tcttgtgcgc ttcagacggc accgctattc gggcggcaga cggcatgttg tccgaatgtc
    17101 tgctccgcct ttgaatctgc cggtatgcct gctatccgcc cgacttttca aaacaggttc
    17161 cgacgattcc gcacgcgcct gccgcctttg ccaagccgta caggatttcc tgcggcatat
    17221 cgcggttcca taatcccgta atattcgcaa tcacgggcag atggctgatt tggcggactt
    17281 tcacgatgga ttcgacatcc aaacggtagg gatggtcttt ggtatggctc aacacgcccg
    17341 actcgcccag aatcaggtgg cggttgccgc gcgagacaac atattctgcg gcattcaacc
    17401 aatcttcggc agaatggtgc ttgtctttac acagcaccaa gggaatgttc aggcttccgg
    17461 cttcattcaa tacggcaaga tcggacatca gcccgccgcc caaatacagg atgtccgccc
    17521 ccgcattcaa agccgcttcg acatggcaga cgttgcggac gcgcaccaat acgggtttcc
    17581 ctgcgccatg cgccgatgcg gtctgttccg ccaaccgtct gcaccgtccc cgcccttcat
    17641 ccgcacttga agtgtcgtat aagtttgccg aagtgaaaaa cggatctaga aacactgcat
    17701 ccgcattgcg ccatactgac ggttctgcgg cgatacggac ggtttccccg ccgccgaaag
    17761 ccacgccttt ggcggcaacg cggctgtctt ccgcccgatt ttcccgactg acggttttcc
    17821 atgtatccaa aatgcggacg gctttctcga cctccggcag cgtctgcacc tccctgacgc
    17881 tcaaaaccct atcgtcgccg attgcgccga tgacggtacg ctcgtcgccg tgagaaatgt
    17941 gttctcgcag acctctgctg cggataaagg cgacaacgcc ggcaatgtcc gcttcggcgg
    18001 cacgcctgct catgacaata atcatatttc ctcctgacac aagaaacggc ctgcccaaaa
    18061 taggattttt gcaagccgtg ttatactgtg gcgtgtttta cagattgttc gggctatgga
    18121 tttattatcg gttttccaca aataccgtct gaaatatgcg gtggccgtgc tgacgatact
    18181 gcttttggcg gcagtcgggc tgcacgcttc cgtatatcgc accttcacgc ctgaaaacat
    18241 ccgcagccgc ctccaacaaa gcattgcaca cacacaccgg aaaatctcgt ttgatgcgga
    18301 cattcagcgc aggctcctgc cccggccgac cgtcatcctg aaaaacctga ccattaccga
    18361 acccggcggc gaccagactg ccgtttccgt ccaagaaacc aaaatcggat tgagctggaa
    18421 aaacctgtgg tcggatcaga tacagattga aaaatgggtg gtttcgagtg cggaacttgc
    18481 cctgacgcgc gacgggaaag gtgtttggaa catccaagac ctgatcgaca gccaaaaacg
    18541 ccaagcctca gtcaaccgca ttatcgtcga aaacagcacc gtccgcctca atttcctgca
    18601 ggaacagctt atcctgaagg aaatcaacct caacctgcaa tcccccgatt cgtcggggca
    18661 gccgtttgaa agttcgggca tactggtttg gggaaagctg tccgtcccgt ggaaaagcag
    18721 ggggctgttc ctttcagatg gcatcggcac gcccaaaatc tcaccgttcc attttgaagc
    18781 ttccacttcg ctggacggac acggcatcac catttccacc accggcacac ccgccatccg
    18841 tttcaacgcc ggcggagcgg ataccgccgg cctcggcctg cgtgcagaca cttccttccg
    18901 caacctccac ctgaccgcgc aaatccccgc gctggcactc aggaacaaca gcattaaaat
    18961 tgaaaccgtc aacggcgcat ttaccgccgg cggcgaatat gcccaatggg acggttcgtt
    19021 caaactcgac aaagccaacc tgcactccgg catcgccaac atcggcaacg ccgaaatctc
    19081 cggcagcttc aaaacaccgc gccaccagac caacttctcc ctcaattcgc cgctcgtatg
    19141 gacggaaaac aaagggctgg acgcgccgcg cctgtatgta tcgacccttc aggataccgt
    19201 caaccgcctg ccgcaacccc gtttcatcag ccggctcgac ggttcgctgt ccgtaccgaa
    19261 tctgcaaaat tggaatgccg aattaaacgg cacattcgac cgccaaaccg ttgccgcgaa
    19321 attcagatac acacatgaag acgcaccgca tctggaagcc gccgtcgcac tgcaaaaatt
    19381 gaacctgacc ccctatcttg acgacgtgcg gcaacaaaac ggcaaaatat ttcccgacac
    19441 cctcgccaag ctgtccggcg acatcgaggc gcacctgaaa atcggaaaag tccaacttcc
    19501 cggcctgcaa ctggacgata tggaaaccta cctccacgcc gacaaaggcc atatcgcgct
    19561 cagccgtttc aagtcagggc tttacggcgg ccataccgaa ggcggcatca gcatcgccaa
    19621 cacccgtccc gccacttacc gcctgcaaca gaatgcaagc aacatccaaa tccaaccgct
    19681 gctgcaagac ctgttcggct tccacagctt cagcggcaac ggcgatgcgg tcatcgacct
    19741 gaccgcgggc ggcgaaaccc gaaaagagct tatccgctcg cttcagggca gcctgtcgct
    19801 gaatatttcc aacggcgcgt ggcacggtat cgacatggac aatatcctga aaaacggcat
    19861 ttcgggcaaa actgccgaca atgccgcacc cagcacaccc ttccaccgat tcacgctcaa
    19921 cagcgaaatt tcagacggca tcagccgcca catcgatacc gaactcttct ccgacagcct
    19981 ctatgttacc agcaacggct ataccaatct ggatacgcag gaattgtctg aagatgtcct
    20041 tatccgcaac gccgtccatc cgaaaaacaa accgattccc ctgaaaatca ccggtacggt
    20101 ggacaaaccg tccattaccg tcgattacgg caggctgacc ggcggcatca attcgcgcaa
    20161 agagaaacag aaaatcctcg aagacaccct gctggaacaa tggcagtggc tcaaacctaa
    20221 agaaccgtaa acatcctgcg tacacaaatg ccgtctgaaa cgcccccacg cttcagacgg
    20281 cagaccgtaa aacctacaac cccaattcct cccaaatccc atcaatctta gccgtaaccg
    20341 cagggtcttt tttgatgacg ctcccccatt cgcggtcggt ttcgcccggc catttgttgg
    20401 tcgcgtccaa acccattttg ccgccgagtc cgctgacggg gctggcgaag tcgagatagt
    20461 cgatgggcgt gttttctacc aaaacagtgt cgcgcacagg gtccatgcgc gtggtgaccg
    20521 cccagatgac ttctttccag tcgcgcacgt ccacatcgtc atccaccacg atgatgaatt
    20581 tggtgtacat aaactggcgc aggaacgacc agcagcccat catcacgcgt ttggcgtgtc
    20641 cggcgtactg ttttttcatg ctcaccaccg ccatgcggta ggagcagcct tcgggcggca
    20701 ggtagaaatc ggtgatttcg ggaaactgct tttgcaagag cggtacgaac acttcgttca
    20761 acgccacgcc caaaacggca ggctcatcgg gcggtttgcc tgtgtaggtc gaatggtaaa
    20821 tcgggttttc gcgcatggtg atgcgttcga ccgtaaacac agggaaataa tcctgctcgt
    20881 tgtaatagcc cgtgtggtcg ccgtacgggc cttccaacgc ggtttcgttc ggatgaatca
    20941 caccttccaa cacgatttct gcgcgtgcag gcacttgcaa atcgttgccg atacatttca
    21001 ccagctccgt ccgcgaaccg cgcagcagtc cggcaaactg gtattcgctc aaggtatcgg
    21061 gaacaggcgt taccgcgccc aaaatggtgg cagggtcgca gccgagcacg acggcgacgg
    21121 gatacggcgt atcgggattg agtttgcgga attcctgata atccaacgcg ccgccgcgat
    21181 gcgacagcca gcgcataatc agtttgtttt tgccgattaa ttgttggcgg taaatgccta
    21241 agttttggcg ttttttgtgc ggcccgcgcg ttacggtcaa gccccacgtc accagcggcg
    21301 caacgtcttc cggccagcaa tgctgaatcg gaagttgata caaatcaacg tcttcgcctt
    21361 cccacacgat ttcctgacac ggcgcgtttt tcaccacgtt cggcgccatg ctccaaatgt
    21421 ctttcagtag cggcagtttg gaaaacgcat ctttgatgcc tttgggcggt tcgggttctt
    21481 tcaaatacgc cagcgtctgc ccgatttcac gcagcttgga cacgctgtcc gcgcccatgc
    21541 ccatcgccac acgttcgggc gtgccgaaca ggtttgccaa cacaggataa ccgtagcgcg
    21601 taccgtcagg cttaatcgga ttctcaaaca acaatgccga cccttcggcg cgcagcacgc
    21661 ggtcggcgat ttcggtcatt tccaaatgcg gggaaacggg gtgcgcgatg cgtttgagtt
    21721 tgccctgctg ctcgagcatg gcgatgaagt cgcgcaggtc tttgtatttc atattcatcc
    21781 tttttgtcct tttatcctga gcaatccgat tcggataccg cccctatcct tgcctgtgcc
    21841 tcggcatatt ctatgccgtg ataaaagtcg cgtaccagcg gatgtccgcc gctttgatga
    21901 agttgcagca aagggcgttg accatcgggt tgggtaacga cattgcaatg caaaccgaag
    21961 gtgtcggatt cgtaaggggg cagccggttg cagatcatcc cgaaataaac gacattttcc
    22021 ggattgtcgt gaaagtggct ttgatagtcg ttaaaactct tttcgctgac ggacacccac
    22081 acgccgtatt ccagcgtttc ctgatgcccg ataatcggaa tcggcagcac cgcgcggata
    22141 aagcggtcgg tttggtcggg atagcggatg atgcagaaat cagaatcgca ttctgcttga
    22201 taagcaatgc gttcttcttc actgagttga ttatagggat cgggggcggt aaagccgatt
    22261 gcgggcatat cttcatgttt ttcgccgcag gaagtgcaag tgtacataag gtttcagact
    22321 ttcaaaacga gtttgcggta aagccattcg ccggcaaaca gcatccccat caatacatag
    22381 gcaatcacgc ctgtataaac cgcccaccaa tcatatcgcc ccaaccgagc caacaaagcg
    22441 gtaagcgtcc cgttgattat aaaaaatacg caccaaacct gcgttacccg gcgggtatag
    22501 cgcacggctt tttcaggcag gtcgggctgt tgcagccgcg caagtttttc aatcaccgtc
    22561 tgtccggcaa acaggctgcc gccgaacacc gccaacatca tcagattgac gaggacggga
    22621 taccaataca tcgaatcatg ccgtctgaac accaatactg cggcaaaaaa cagtgtaata
    22681 aacaaagccg cataacgctg ttggggcagt ttggcagtca gggcgcgcag cagccacaac
    22741 ccgcacatcg ccgccgccag ccagacaaac cagcccgcct ccctgccgta atatagtgga
    22801 ttaacaaaaa ccagtacagc gttgcctcgc cttgccgtac tatctgcggc ttcgtcgcct
    22861 tgtcctgatt tttgttaatc cactatacca caaagcggga taggcaatgc ttaatacggt
    22921 cagaaaaata tgtccgaaaa aaccgggttt cattttgaat ccgcacaaat gttttcagac
    22981 ggcatccgat aaaaacatgc cgtctgaaaa ataattagaa atacccgatt agcccgcctg
    23041 aatcttcaat accgcctgta ccacgtcgtt gacggtgcgg acattgcgga aatcttcagc
    23101 ctgcagcttg cgggctgcca cagccattca cgattgcgat atacccggcg gcattcccga
    23161 ctgccgttca gcagaaagcg cacccattcc aaaccgctcc aatatgcctc gggcagcata
    23221 ccggcttcag acggcatatc gtccgaagca gataaagtca ggctgtaacg cgtccctttg
    23281 gtcagaacca tcgccaaagc ataagcaaac ggcgcgcgcg ttgccgatac ggcatatcct
    23341 tccggcagcg gatcgtccgc cgccaaaacc aaaaccgacc cgcatccctc ttccaacagt
    23401 gatgccgctt ccgccaatgc cgtttccaca ccgtcggcac acacggccaa tgccgtctgc
    23461 tcgctcatat cccgcctcaa tatcgaccat taccccgccg ttgcattatg caccgacaaa
    23521 ccgaacgaag tcggcgacac ggtatgcgat ttcaacagtt ccaaccacaa atcgaaactg
    23581 cgcgccattt ccccgtcgtg cgaggcataa actatagtgg attaaatttt aggggctgtg
    23641 ctagattagc agatatgtta ccctcgaaat atgaagataa cacactgcaa attaaagaaa
    23701 aaagtacaga aagaactgct ccgttttttt gtactcgaag ttaccgcccg ttctgccgcc
    23761 gatattttgg gtatccatcc caattcggca gcactgctct accgtaaaat ccgcacggtt
    23821 accaactatc atttggcctt ggctgccgat gaggtttttg agggccctgt cgagcaggac
    23881 gaaagcgatt tcggcggacg gcgtaaaggc agacgtggtc gcggtgcggc aggaaaagtg
    23941 gttgtcttcg gcattctgaa acgcaacgga cgggtctata ccgttgtggt ggataatgcc
    24001 aagtctgaaa cgttactccc tgtcatcaag aagaaaatca tgccggacag cattgtttat
    24061 accgatagtc tgagcagctg cgacaagttg gacgtgagcg gttttatcca ttaccgcatc
    24121 aaccattcca aggagtttgc agaccgtcaa aaccacatta acggcattga gaacttttgg
    24181 aatcaggcaa aacgcgtctt gcgaaaatat agtggattgg cacgaacgtt tccccgaatt
    24241 ggaagcgcaa ctgggtgcgc cgattgagga ttacgcgccg ccgaaacatt ggacgcgcaa
    24301 gcagctcaga agtatggggc gtgtgtcgta tttgtgcgtc gatgcggcgg agcaggcttt
    24361 gacggatgcc ggtttgctcg gagacgaaag cattactgac ggacgcatgg gcgttgcctg
    24421 cggctcttcc agcggcagca ccaaagacat cggcgatgta ggcgaattgt tgctgaccgg
    24481 catgtcgcgc aacttcagtg ccaacaccta tgtgcgtatg atgccgcaca ccgccgccgc
    24541 caatatcggc atctttttcg ggctgaaagg acgcatcatc ccgacatcga gcgcgtgttc
    24601 gtccggcagc caaggcatag gttatgccta cgaagccatc aaatacggtc tgaccgatat
    24661 gatgctggcg ggcggaggcg aagaattttt cccgtccgaa gtgtatgttt tcgactcgct
    24721 ttatgccgcc agccgccgca acggcgaacc ggaaaaaacc ccgcgcccat acgacgcgaa
    24781 ccgcgacggg ctggtcatcg gcgaaggcgc ggggattttc gtgctggaag aattggaaca
    24841 cgccaaacgg cgcggtgcga taatttacgc cgaactcgtc ggctacggag ccaacagcga
    24901 tgcctaccat atttccacgc cccgccccga cgcgcaaggc gcaatccttg cctttcagac
    24961 ggcattgcaa cacgcaggtc ttgcgcccga agacatcggc tggattaatc tgcacggcac
    25021 cgggacgcac cacaacgaca gtatggaaag ccgtgccgtt gcagcggttt tcggcaacaa
    25081 tacgccctgc acgtccacca agccgcaaac cggacacacg ctgggtgcgg cgggcgcaat
    25141 cgaagccgcg ttcgcgtggg gcattgccga ccggcaaagc aatcccgaag gaaaacttcc
    25201 gccccagctt tgggacgggc agaacgatcc gaaccttccc gccatcaacc tgaccggcag
    25261 cggcagccgc tgggaaaccg aaaaacgcat taccgccagc tcgtcgtttg ccttcggagg
    25321 aagcaactgc gtcttaatca tcggatgaaa taagtttgtc aatcccaccg ctatgctata
    25381 caatacgcgc ctactcttga cgggtctgta gctcaggggt tagagcaggg gactcataat
    25441 cccttggtcg tgggttcgaa ccccaccgga cccaccaatt cccaagcccg gacgtatgtt
    25501 tgggcttttt tgccgccctg tgaaaccaaa atgctttgag aaaccttgat aatgaaaaaa
    25561 gtcagcgtat tgattgttgc caaaaacgaa gcaaaccaca ttcaggaatg tattgaaagt
    25621 tgccgtttcg ataaagaagt tatcgttatc gacgactaca gcaccgacaa tactgccgaa
    25681 attgccgagg gtttgggcgc aaaagtcttc agacggcatt tgaatgggga tttcggagcg
    25741 caaaaaacat ttgccatcga acaggcaggc ggagaatggg ttttcctgat tgatgcagac
    25801 gaacgctgca cgccggaact atctgatgaa atctcaaaaa ttgtccaaac cggcgattat
    25861 gccgcctatt ttgtcgaacg ccgcaacctt ttccccaacc atcccgccac acacggcgcg
    25921 atgcgtcccg acagcgtatg ccgtctgatg ccgaaaaaag acagttcggt gcaaggcaaa
    25981 gtacacgaaa ccgtacaaac cccctacccc aaacgccgtc tgaagcattt tatgtaccat
    26041 tacacgtacg acaactggga acaatatttc aacaagttca acaaatatac ttccatttca
    26101 gccgaaaaat accgagagca gggaaagccc gtgcgtttcg ttagggacat tatcctccgc
    26161 ccgatttggg ggtttttcaa aatttatatc ctgaacaaag ggtttcttga tggaaaaatg
    26221 ggttggatta tgtccgtcaa ccacagctat tacacgatga ttaaatatgt caaactatat
    26281 tatctgtaca aatccggcgg aaaattttaa atggaaaaag aattcaggat attaaatatc
    26341 gtatcggcca agatttgggg tgggggcgaa cagtatgtct atgatgtttc aaaagcattg
    26401 gggcttcggg gctgcacgat gtttaccgcc gtcaataaaa ataatgagtt gatgcacaga
    26461 cgcttttccg aagtttcttc cgttttcaca acgcgccttc acacgcccaa cgggctgttt
    26521 tcgctctacg cacttacccg ctttatccgg aaaaaccgca tttcccacct gatgatacac
    26581 accggcaaaa ttgccgcctt atccatactt ttgaaaaaac tgaccggagt gcgcctgata
    26641 tttgtcaaac ataatgtcgt cgccaacaaa accgattttt atcaccgcct gatacagaaa
    26701 aacaccgacc gctttatttg cgtttcccgt ctggtttacg atgtgcaaac cgccgacaat
    26761 ccctttaaag aaaaataccg gattgttcat aacggtatcg ataccggccg tttccctccc
    26821 tctcaagaaa aacccgacag ccgttttttt accgtcgcct acgccggcag gatcagtcca
    26881 gaaaaaggat tggaaaacct gattgaagcc tgtgtgatac tgcatcggaa atatcctcaa
    26941 atcaggctca aattggcagg ggacggacat ccggattata tgtgccgcct gaagcgggac
    27001 gtatctgctt caggagcaga accatttgtt tcttttgaag ggtttaccga aaaacttgct
    27061 tcgttttacc gccaaagcga tgtcgtggtt ttgcccagcc tcgtcccgga ggcattcggt
    27121 ttgtcattat gcgaggcgat gtactgccga acggcggtga tttccaatac tttgggggcg
    27181 caaaaggaaa ttatcgaaca tcatcaatcg gggattctgc tggacaggct gacacctgaa
    27241 tctttggcgg acgaaatcga acgcctcgtc ttgaaccctg aaacgaaaaa cgcactggca
    27301 acggcaggac atcaatgcgt cgccaaccgt tttaccatca accataccgc cgacaaatta
    27361 ttggatgcaa tataagccgc tttcagacgg catatgccgt ctgaaagcct ttgatgcaac
    27421 aaaccgccaa attatattcg ttcattggaa agaaacaccc cgaatccatc cttaaaaata
    27481 agaaaatccc aatatccccc gatattacgc agcctattgg caaagttttg cagcgtcttc
    27541 cccggcttgt gctgccgcat caagtgcttt gttacaatgt atagtaga
//
LOCUS       DAXHSF010000008        26399 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.008, whole genome shotgun sequence.
ACCESSION   DAXHSF010000008 DAXHSF010000000
VERSION     DAXHSF010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26399
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.008"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            716..2164
                     /gene="gnd"
                     /locus_tag="ACFM8S_000260"
     CDS             716..2164
                     /gene="gnd"
                     /locus_tag="ACFM8S_000260"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HFC6313393.1"
                     /translation="MKGDIGVIGLAVMGQNLILNMNDCGFKVVAYNRTIGKVDEFLNG
                     AAKGTGIIGAYSLQDLVDKLAKPRKIMMMVRAGSVVDDFIEQLLPLLDEEDIIIDGGN
                     ANYPDTTRRTHYLAEKGILFVGAGVSGGEEGARHGPSIMPGGDKRAWEAVKPIFQAIA
                     AKTPQGEPCCDWVGKDGAGHFVKMVHNGIEYGDMQLICEAYQFMKDGLGLSYDEMHRV
                     FAEWNKTELDSYLIEITAAILGYKDEGGEPLVEKILDTAGQKGTGKWTGINALDLGIP
                     LTLISEAVFARCVSSFKEQRVQTGKLFARTVTPVEGGKQEWVEALRQALLASKIISYA
                     QGFMLIREAGESYGWDLDYGNTALLWREGCIIRSAFLSNIRDAYENNPDLVFLGADPY
                     FKNILENCLPAWRKVVAKAVECGIPMPCMASAITFLDGYTTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGKGGDTASTTYDI"
     gene            2227..3498
                     /gene="waaA"
                     /locus_tag="ACFM8S_000261"
     CDS             2227..3498
                     /gene="waaA"
                     /locus_tag="ACFM8S_000261"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFC6313394.1"
                     /translation="MFQWLYDALWLLAPIWIRRYLDKRSGSAPAYRAHRDERFGKPYP
                     NPVTGAVWIHAVSVGETRAAQPLIRELRQRFPDAPLLMTQMTPTGRETAQVLFPDAQC
                     RYLPYDKKTWVRQFLREHRPMFGILMETEIWPNLMKECRRAGVPLFLANARLSEKSLN
                     GYLKVRRLIRPAAASLTGCLAQTEADAARLAKLGAASVQVCGNTKYDLMPSEQMKTLA
                     GQFEKRIGGRPVAVCGSTRVYRGEDEAEKLLAAWQQYRGDALLVVVPRHPEHFQTVFE
                     TAKRFGFKVQRRSDGLPVEPDTQVWIGDSMGELYAYYLCADVAFVGGSLVDSGCQNII
                     EPLSCGVPTIFGFSTYNFSEACRHALASGAAVRVESADAWREAVEKTLSSEGGGMQMQ
                     AHVDGFIAQHRGAGARIAEAVREAVCGHRGR"
     gene            3539..3988
                     /locus_tag="ACFM8S_000262"
     CDS             3539..3988
                     /locus_tag="ACFM8S_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family copper resistance protein"
                     /protein_id="HFC6313395.1"
                     /translation="MSIYAVAHIVHLYCAIAFVGGVFFEVLVLSVLHTGRVSCEARRE
                     VEKAMSYRAVRVMPFVVGLLFASGVVMAANRYLSILGEPFATSFGTMLTLKILLAFSV
                     LAHFAIAVVKMARSTLTVGWSKYIHAVVFTHMLLIVFLAKAMFYISW"
     gene            4021..4995
                     /locus_tag="ACFM8S_000263"
     CDS             4021..4995
                     /locus_tag="ACFM8S_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFC6313396.1"
                     /translation="MTEYPGIGSPLFYGVFFAAVLVMIALDMFSLKKNGSHKVGVKEA
                     LAWSGLWVAVSCLFAGWLYFELAGNLGYGAAVAKEKVLEFFTGYILEKSLAVDNIFVF
                     LMIFGYFKVAPQFQHRVLLYGVLGALVLRTVMIFVGAALVQQFEWILYLFGAFLLYTG
                     IHMMKPEGDEKEDLANSRLLNAVKKVVPVGTEFHGEKFFTVENGKKIATPLFLVLVMI
                     ELSDVVFAVDSIPAVFAVTTDPFIVLTSNIFAILGLRAMYFLLADVAERFVFLKYGLA
                     FVLGFIGVKMLIMHWVHIPISVSLSVVFGALGASVLTSLVYTKKQSDK"
     gene            5064..5139
                     /locus_tag="ACFM8S_000264"
     tRNA            5064..5139
                     /locus_tag="ACFM8S_000264"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5097..5099,aa:Lys,seq:ttt)
     gene            5358..6611
                     /gene="murA"
                     /locus_tag="ACFM8S_000265"
     CDS             5358..6611
                     /gene="murA"
                     /locus_tag="ACFM8S_000265"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFC6313397.1"
                     /translation="MDKLKISANGPLNGEITVSGAKNAALPLMCAGLLTSGTLRLKNV
                     PMLADVKTTQKLLQGMGARVLTDNISEFEINGGTVNNTCAPYELVRTMRASILVLGPT
                     LARFGEAQVSLPGGCAIGSRPVDQHLKGLEAMGAEIVIEHGYVKAKGKLKGTRVAMDV
                     VTVGGTENLLMAATLAEGTTVLENCAIEPEVVDLAECLVKMGAKISGIGTSTMVVEGA
                     GELHGCEHSVVPDRIEAGTFLCAVAITGGRVVLRNAAPKTMEVVLDKLVEAGAVIEAG
                     DDWIAIDMRQRPKAVDIRTVVHPGFPTDMQAQFMALNAVAEGSCRVVETIFENRFMHV
                     PELNRMGANITTEGNTAFVQGVERLSGAVVKATDLRASASLVIAGLAARGETVVEQIY
                     HLDRGYENIEKKLGSVGAKIERVSG"
     gene            6692..7870
                     /locus_tag="ACFM8S_000266"
     CDS             6692..7870
                     /locus_tag="ACFM8S_000266"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFC6313398.1"
                     /translation="MAFLKLTEQNVQGKTVLIRADMNVPFKDGKISDDTRIRASLASI
                     QYCMDNGASVIVMTHLGRPTEGEFHPEDDVAPVAAHLGGLLGKNVKVLNDWRENKPAL
                     NAGDVVMLQNVRINKGEKKNDLELGKAYASLCDVFVNDAFGTAHRAQASTEAVAQAAP
                     VACAGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGI
                     ANTFLLAEGKAIGKSLAEHDLVEESKKIMAKMADKGGSVPLLTDVVVAKAFAADAEAV
                     VKDIADVAEDDMILDIGPKSAAELAELLKAAGTVVWNGPVGVFEFDQFAGGTKVLAEA
                     IAQSKAFSIAGGGDTLAAIAKFGVTDQIGYISTGGGAFLEFLEGKELPAVAALEKRGA
                     "
     gene            complement(7937..8185)
                     /locus_tag="ACFM8S_000267"
     CDS             complement(7937..8185)
                     /locus_tag="ACFM8S_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HFC6313399.1"
                     /translation="MLTSEQVKAMIEGVAKCEHIEVEGDGHHFFAVIVSSEFEGKARL
                     ARHRLIKDGLKAQLESNELHALSISVAATPAEWAAKAQ"
     gene            complement(8276..9193)
                     /gene="ftsX"
                     /locus_tag="ACFM8S_000268"
     CDS             complement(8276..9193)
                     /gene="ftsX"
                     /locus_tag="ACFM8S_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFC6313400.1"
                     /translation="MSIIHYLSLHVESARTALKQLLRQPFGTLLTLIMLAVAMTLPLF
                     MYLGIQSGQSVLGKLNESPQITVYMETAAAQSDSDTVRSLLARDKRLDNIRFISKEDG
                     LEELQSNLDQNLISMLDGNPLPDVFIVTPDPATTPAQMQAIYRDITKLPMVESANMDT
                     EWVQTLYQINEFIRKILWFLSLTLGMAFVLVAHNTIRLQILSRKEEIEITKLLGAPAS
                     FIRRPFLYQAMWQSILSAAVSLGLCGWLLSAVRPLVDAIFKPYGLNIGWRFFYAGELG
                     LVFGFVIALGVFGAWLATTQHLLGFKAKK"
     gene            complement(9190..9840)
                     /gene="ftsE"
                     /locus_tag="ACFM8S_000269"
     CDS             complement(9190..9840)
                     /gene="ftsE"
                     /locus_tag="ACFM8S_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFC6313401.1"
                     /translation="MIRFEQVSKTYPGGFEALKNVSFQINKGEMIFIAGHSGSGKSTV
                     LKLISGITKPSRGKILFNGQDLGTLSDNQIGFMRQHIGIVFQDHKILYDRNVLQNVIL
                     PLRIIGYPPRKAEERARIAIEKVGLKGRELDDPVTLSGGEQQRLCIARAVVHQPSLLI
                     ADEPSANLDRAYALDIMELFKTFHEAGTTVIVAAHDETLMADYGHRILRLSKGRLA"
     gene            complement(9837..10028)
                     /locus_tag="ACFM8S_000270"
     CDS             complement(9837..10028)
                     /locus_tag="ACFM8S_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313402.1"
                     /translation="MPSESASDGIFPARPSVSAKLIHYLKTGGIFIIGTVLPIVQTAY
                     PCGRNRPKRYAAHSLQDLL"
     gene            complement(10036..10518)
                     /locus_tag="ACFM8S_000271"
     CDS             complement(10036..10518)
                     /locus_tag="ACFM8S_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFC6313403.1"
                     /translation="MKYLNLAAITLAATFAAHTASADELAGWKDNTPQSLQSLKAPVR
                     IVNLWATWCGPCRKEMPAMSKWYKAQKKGSVDMVGIALDTSDNIGNFLKQTPVSYPIW
                     RYTGANSRNFMKTYGNTVGVLPFTVVEAPKCGYRQTITGEVNEKSLTEAVKLAHSKCR
                     "
     gene            10642..10995
                     /gene="arsC"
                     /locus_tag="ACFM8S_000272"
     CDS             10642..10995
                     /gene="arsC"
                     /locus_tag="ACFM8S_000272"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271974.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFC6313404.1"
                     /translation="MSEIKIFHNPRCSKSRAALSLLEERGIAAEVVKYLDTPPDLSEL
                     KDIFNKLGLESARGMMRVKDDLYKELGLDNPDLDNDALLRAIADHPALLERPIVLANG
                     KAAVGRPLENIEAVL"
     gene            10992..11648
                     /locus_tag="ACFM8S_000273"
     CDS             10992..11648
                     /locus_tag="ACFM8S_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFC6313405.1"
                     /translation="MKKRLFCSRNGLRYYLLGGFCLSVFPLLLVFASSVWAVYRTGGQ
                     VLPPYVRADAALVLGAAAWDKRPSPVFRERINHAIALYQSRRVGKIIFTGGRSKKGYM
                     TEAEVGRRYALKQGIPPRNILFENTSRNTYENLNNIRPVLRANGIASVVIVSDPYHLA
                     RAAEMAEDLGVRVYMSATPTTRFDAGNKKKIFMLQEGYALSLYRLEKWGRDFFNWLFH
                     "
     gene            11841..13235
                     /gene="gltX"
                     /locus_tag="ACFM8S_000274"
     CDS             11841..13235
                     /gene="gltX"
                     /locus_tag="ACFM8S_000274"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFC6313406.1"
                     /translation="MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDT
                     DLARSTAESVNIILDGMKWVGLDYDNADNVVYQTRRFERYKEVIAELLAKGDAYYCYC
                     SKEELEAMREKAEKEGTATYDRRWRPEAGKTLPEIPAGVQPVVRFKTPLDGVTKWADL
                     VKGEISIPNEALDDLIIARADGTPTYNFCVVVDDYDMGVTHVIRGDDHVNNTPKQINI
                     LKAIGATLPEYGHLPMILNEQGKKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWA
                     HGDDEFFTMEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKITPNGKLAELVKPRLA
                     LRDIHETEKPALEDVLAQVKDRAQDLNALADECLYFYKKQVPAEADVAKHWDDEAAAR
                     MLRFAERLEGLEDWNTEAIHDLFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVL
                     ALIGKEEVLKRIRA"
     gene            13256..13651
                     /locus_tag="ACFM8S_000275"
     CDS             13256..13651
                     /locus_tag="ACFM8S_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313407.1"
                     /translation="MPSDVGIRLQTAFKRKFKMKKIFYFLMVVFSTSVWAGDAEDNLL
                     SIQSGYRALLQKQNNLDGKIIGMQSDLEDARRRLQAAQADINRLEAEIPAAMAQKARQ
                     AEELRQIGVRLDHAWNAVYGAGGTKASGN"
     gene            13655..14146
                     /locus_tag="ACFM8S_000276"
     CDS             13655..14146
                     /locus_tag="ACFM8S_000276"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC6313408.1"
                     /translation="MNSLFVDNTVFITRLKAGHIGRLVQALFEEWHGFEPWSSVDKIR
                     AYYGRCLKDDELPLAFAAVDDFGTLLGSAAVKRHDMESFPQYEYWLGDVFVLPEYRGK
                     GIGRRLVAHCIGAARSLGIKFLYLYTPDVQIFYESFGWAVVGRHFHNGEWVTVMRLDM
                     DKV"
     gene            complement(14270..16864)
                     /gene="mutS"
                     /locus_tag="ACFM8S_000277"
     CDS             complement(14270..16864)
                     /gene="mutS"
                     /locus_tag="ACFM8S_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFC6313409.1"
                     /translation="MSKSAVSPMMQQYLGIKAQHTDKLVFYRMGDFYEMFFDDAVEAA
                     KLLDITLTTRGQVDGEPVKMAGVPFHAAEQYLARLVKLGKSVAICEQVGEVGAGKGPV
                     ERKVVRIVTPGTLTDSALLEDKETNRIVAVSPDKKYIGLAWASLQSGEFKTKLTTVDK
                     LDDELARLQAAEILLPDSKNAPQLQTASGVTRLNAWQFAADAGEKLLTEYFGCQDLRG
                     FGLDGKEHAVAIGAAGALLNYIRLTQNLMPQHLDGLSLETDSQYIGMDAATRRNLEIT
                     QTLSGKKSPTLMSTLDLCATHMGSRLLALWLHHPLRNRAHIRARQEAVAALESQYKPL
                     QCRLKSIADIERIAARIAVGNARPRDLAALRDSLFALSEIELSAECSSLLGTLKAVFP
                     ENLSTAEQLRQAILPEPSVWLKDGNVINHGFHPELDELRRIQNHGDEFLLDLEAKERE
                     RTGLSTLKVEFNRVHGFYIELSKTQAEQAPADYQRRQTLKNAERFITPELKAFEDKVL
                     TAQEQALALEKQLFDGVLKNLQTALPQLQKAAKAAAALDVLSTFSALAKERNFVRPEF
                     ADYPVIHIENGRHPVVEQQVRHFTANHTDLDHKHRLMLLTGPNMGGKSTYMRQVALIV
                     LLAHTGCFVPADAATIGPIDQIFTRIGASDDLASNRSTFMVEMSETAYILHHATEQSL
                     VLMDEVGRGTSTFDGLALAHAVAEHLLQKNKSFSLFATHYFELTYLPEAHAAAVNMHL
                     SALEQGQDIVFLHQIQPGPAGKSYGIAVAKLAGLPVRALKSAQKHLNGLENQAAANRP
                     QLDIFSTMPSEKGDEPNVGNFVDKAEEKHFEGILAAALEKLDPDSLTPREALSELYRL
                     KDLCKSVS"
     gene            17306..18310
                     /gene="gap"
                     /locus_tag="ACFM8S_000278"
     CDS             17306..18310
                     /gene="gap"
                     /locus_tag="ACFM8S_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFC6313410.1"
                     /translation="MSIKVAINGFGRIGRLALRQIEKAHDIEVVAVNDLTPAEMLLHL
                     FKYDSTQGRFQGTAELKDDAIVVNGKEIKVFANPNPEELPWGELGVDVVLECTGFFTN
                     KTKAEAHIRAGARKVVISAPGGNDVKTVVYGVNQNILDGSETVISAASCTTNCLAPMA
                     AVLQKEFGVVEGLMTTIHAYTGDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPVATGSLTELVSVLERPVTKEEINAAMKAAASESYGYNEDQ
                     IVSSDVVGIEYGSLFDATQTRVMTVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFAGKI
                     "
     gene            complement(18380..18499)
                     /locus_tag="ACFM8S_000279"
                     /pseudo
     CDS             complement(18380..18499)
                     /locus_tag="ACFM8S_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(18568..19248)
                     /gene="tsaA"
                     /locus_tag="ACFM8S_000280"
     CDS             complement(18568..19248)
                     /gene="tsaA"
                     /locus_tag="ACFM8S_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFC6313411.1"
                     /translation="MTYTITPIGTARSPYKQKFGIARQPGLVSAAKACIELNPEFTAD
                     SVRGLEHFDYVWISFIFHGVLDEGWAQMVRPPRLGGKQKMGVFATRSPHRPNHLGLSL
                     LKLERIETGKPVRLYCSGADLLDGTPIVDIKPYIPFVESKPDAASGFVSGKPVELEVV
                     WQENIGAENLSANTKNLISQSIAQDPRPAYQNIPERIYVMNIADYEVRFQIEENRATV
                     IDLSPTPL"
     gene            19362..19997
                     /locus_tag="ACFM8S_000281"
     CDS             19362..19997
                     /locus_tag="ACFM8S_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HFC6313412.1"
                     /translation="MIVSIDVDAQKTFTPLCPDELPVNEGHLIVEELNAQAALADLRV
                     MTKDAHHMVAKWLVDNPVDMLKPTGLPDADLTWVAHAMVGTRGYELLDGLPSAKEYDY
                     CVWKGVDPELHPYGACFHDIEEKLSTGLIEWLRCQNTDTVIIGGLATDYCVKTTVLQL
                     LKGGRWQVVVNEAACRGIAPETIETAWQEMHSAGAIILKNADEINKYINNQ"
     gene            20034..21002
                     /gene="waaC"
                     /locus_tag="ACFM8S_000282"
     CDS             20034..21002
                     /gene="waaC"
                     /locus_tag="ACFM8S_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HFC6313413.1"
                     /translation="MKILLVRLSSMGDLIHTLPAIEDLARQCPDVELHWLCEAGFADI
                     ARLHPFVKKIHVMKWRQWRKHLFRAETWREMGHLKQTLRQEVFDFVLDSQGLIKSACF
                     AKVAKSPICGLDKNSAREGWAALAYVKTYAVPKGKNAVWRNRELFAQVFGYVMPETQV
                     FGLTVPEAGRLKNLAQPYYAALHATSRDSKLWPVENWRSLLQKLNEEQQCNIYLPWGN
                     EDEKTRAKQIADGLPFAIVCDKINLLQAAYLLKYAVGIVGVDTGLLHLANALEKPVVG
                     IYTDTDPIKTGVQVSPIAKNLGNIGQIPTADLVYQALMDCVAADKG"
     gene            21229..21978
                     /locus_tag="ACFM8S_000283"
     CDS             21229..21978
                     /locus_tag="ACFM8S_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EtfB protein"
                     /protein_id="HFC6313414.1"
                     /translation="MKALVAVKRVVDYNVKVRVKADGSDVDIGNVKMSMNPFDEIAVE
                     EAVRLKEAGKVSEIVAVSLGEKKCEETLRTALAMGADRAIHVETDTKLESLAVAKLLK
                     AVADKENPQIFFLGKQAIDDDANQVAQMLAALLNAAQGTFASKVHIEGGEVQIVREID
                     GGEETIALKLPAVISADLRLNEPRFVKLPNIMVAKKKPLEKLTPDDLVADISPRLKTV
                     KFAEPKARQAGVKVASVAELVEKLKNEAKVI"
     gene            21989..22924
                     /locus_tag="ACFM8S_000284"
     CDS             21989..22924
                     /locus_tag="ACFM8S_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFC6313415.1"
                     /translation="MSVLIIVEHDNKQLNPTTLHAVTAAAKLGKIDLLVAGSGASAVV
                     EFAKQVAGVEKVLVADAAHYAEGLAEELAPLVVKLAADYCYVAATATTFGKNLLPRVA
                     ALLDVPQISDLTEIVDNTTFVRPIYAGNAFETVQADSEKLVLTFRATVFDAVAAQGGN
                     AEVINVEATPAQNLSRFVNRQLSHSDRPELTQAKVIVSGGRALGSAEKFNEVLTPLAD
                     VLGAAIGASRAAVDAEYAPNDAQVGQTGKVVAPQLYFAIGISGAIQHVAGMQDSKVIV
                     AINKDADAPIFNVADYGLVGDLFEIVPQLTEVLKN"
     gene            22987..23598
                     /locus_tag="ACFM8S_000285"
     CDS             22987..23598
                     /locus_tag="ACFM8S_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFC6313416.1"
                     /translation="MITTYKTITSSTQAEFKDKGSRFIAFAYPIRTLADVKKYLDPLK
                     EEHHKARHWCYAYRLGVDGMQFRANDDGEPSGSAGRPILGQIDSVGITDVLVVVVRYF
                     GGTLLGVPGLIHAYKEATAQALAVAEVVEKNIEKTVWLKCEYPFLNEAIRIAKQYQAD
                     VLERDLQLDCRLTVSLSLVNYEACVAAWKSTRQIEVNTEKPFE"
     gene            complement(23650..23967)
                     /locus_tag="ACFM8S_000286"
     CDS             complement(23650..23967)
                     /locus_tag="ACFM8S_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HFC6313417.1"
                     /translation="MQKRIDEIQSKYREWCHLLPQLEEDIRRWKHAVALIRDMDNFYT
                     HEYQACHRAIEDGAEVDLRTEGEYSIMSEDALWNALGEFHQLAWLYLRSSVDALDKYT
                     QED"
     gene            24125..25396
                     /gene="purD"
                     /locus_tag="ACFM8S_000287"
     CDS             24125..25396
                     /gene="purD"
                     /locus_tag="ACFM8S_000287"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFC6313418.1"
                     /translation="MKLLVIGNGGREHALAWKLAQSPKVETVFVASGNAGTAIEPKLQ
                     NIDLTAHQDLIEFCRKENIVFTVVGPEAPLAAGVVDDFRAAGLKIFGPTQYAAQLESS
                     KDFAKAFMAKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLAAGKGVIVAMTLD
                     EAHAAIDDMLLDNKMGNAGARIVIEDFLQGEEASFIVMVDGNNVLPMATSQDHKRLLD
                     GDKGLNTGGMGAYSPAPVVTPVVYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQ
                     SGAPHTIEFNCRFGDPETQPIMSRLNSDLSDLVEAAIDGKLDSVTAEWSPQTAVGVVL
                     AAQNYPETPKKGDVISGLDAANQVGKVFHAGTTANEKGDVLTNGGRVLCVVGLGDNVA
                     QAKAKAYGALEKISFDGMQYRKDIADKAINR"
     gene            25430..26032
                     /locus_tag="ACFM8S_000288"
     CDS             25430..26032
                     /locus_tag="ACFM8S_000288"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFC6313419.1"
                     /translation="MTDVLQTVFYIMLFPRIIAASAQRKLSVYLKKGGLVAYPTESCY
                     GLGCLPTLAKALGKLAHLKKRPQHKGMIVIGNQFEQLQPLLQMPSENLQDMLRKEWPA
                     PKTFLLSAKSCVLPELRGKQRSKLAVRVPAHVGARRLCQALQTPLVSTSCNRAGKRAC
                     RTEREVRRQFGRDVWIVGGRIGRQKSPSQIIDGETGKRLR"
     gene            26060..>26399
                     /locus_tag="ACFM8S_000289"
     CDS             26060..>26399
                     /locus_tag="ACFM8S_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313420.1"
                     /translation="MHTQRKNFSDSISLHDCYCTEIKVEGRNVCFNFEDGFVVPDNNP
                     NNQAGKHLKTDFSRVVLTHKNENAEDDYLFDIFEDIVFLGKRLFTVRKFLDFSELLEM
                     VNTQGYFLEFL"
BASE COUNT         6319 a         6051 c         7446 g         6583 t
ORIGIN      
        1 gagcggtttc tgttttttcc ggtaaattcc taaaactcaa aatttcatca ttcccacttt
       61 cgtgggaatg acggaatgta ggttcgtggg aatgacgcgg tacaggtttc cgttacgtat
      121 ccccatccga aagtttactt atacacaagc aatccaaagt tatctgactg aaatattgtg
      181 agatttaccc gcaaatctca ttttttccac cacttataca caaatccgga tgcacactct
      241 gccgatatgc agaaataaga cattcgtgcc gacacctgac catttccaca agttccaaac
      301 acgcttccgg cttaaatctc cacagtattc ccgttcttaa aggcgccagc agccattcgg
      361 taaagaaaag taggatgcca tctgcacaac aagcaaacta tccacaaaac ttattcccca
      421 taaaaaacaa tccacaggta tgcacaaaag ggaatccgcc ttaccgacac cctaaaaaaa
      481 ggtaatcctg atgattttta aaataaaaaa aagttaccca cagaaaattt ctcctatcaa
      541 tataatcatc gtttttttat ttaattttta tattttattt ataagccttc agggaaacgc
      601 acggtaaagc gtaaaggcat cttttcaaaa aattaaagag ttaaggattg ttaaacaagt
      661 gtaaatcagg tagagttgcg gtcgataaat taaatctata acggagcttc ctgcaatgaa
      721 aggcgatatt ggcgtaatcg gtttggcggt tatggggcaa aacctgattt tgaatatgaa
      781 cgattgcgga tttaaggttg tcgcctacaa ccgaacaatc ggtaaggtgg acgaattttt
      841 aaacggcgcg gcaaaaggga cgggcattat cggcgcgtat tccctgcaag acttggtcga
      901 taagctggca aaaccgagaa aaatcatgat gatggttcgc gcaggttcgg tcgtcgacga
      961 ctttatcgaa caactgctgc ccctgttgga cgaggaagac atcattatcg acggcggcaa
     1021 tgccaattac cccgacacga cacggcggac acattacctt gccgaaaaag ggattttgtt
     1081 tgtcggggcg ggcgtatccg gcggcgaaga aggcgcgcgg cacggcccgt ccattatgcc
     1141 gggcggggat aagcgcgctt gggaagccgt caaaccgatt tttcaggcga ttgccgccaa
     1201 aacgccgcag ggcgaaccgt gttgcgactg ggtcggcaag gacggcgcgg ggcattttgt
     1261 caaaatggtg cataacggca tcgaatacgg cgatatgcag ttgatttgcg aagcgtacca
     1321 gtttatgaaa gatggtttgg gattgtccta cgatgaaatg caccgcgtgt ttgccgagtg
     1381 gaacaaaacc gagctggatt cttatctgat tgaaatcaca gcggcaattt tgggctataa
     1441 ggacgaaggc ggtgaaccgc tggtcgagaa aatcctcgat acggcagggc aaaaaggcac
     1501 gggcaaatgg acgggcatca atgccttgga tttgggtatt ccgttgacgc tgatttccga
     1561 agcggtattc gcccgctgcg tttcgtcgtt caaagaacag cgcgtgcaga ccggcaagct
     1621 gttcgcccga accgttacgc ccgtcgaagg cggcaaacaa gaatgggttg aggcgttgag
     1681 acaggccctg cttgcatcca aaatcatttc ctacgcacaa ggctttatgc tgatccgcga
     1741 agcgggcgaa agctacggct gggatttgga ctacggcaac actgcgctgc tgtggcgcga
     1801 ggggtgcatc attcgcagcg catttttaag caatatccgc gatgcctatg aaaacaatcc
     1861 cgatttggtg ttcttgggtg cggatccgta tttcaaaaat attttggaaa actgcctgcc
     1921 ggcgtggcgc aaggtggttg ccaaggcggt cgaatgcggc atccctatgc cctgcatggc
     1981 ttcggcgatt acgttcttgg acggctacac gaccgaacgt ctgcctgcca acctcttgca
     2041 ggcacagcgc gactacttcg gcgcgcacac ttacgaacgc accgacaaac cgcgcggcga
     2101 gtttttccat accaactgga cgggcaaggg cggagatacc gcttcgacaa cctacgatat
     2161 ttgattccgt tgccgaaatg ccgtccgaac cgttcggacg gcatttgttt tgcaggaaat
     2221 agaaagatgt tccaatggct ttatgatgca ttgtggctgc ttgcgccgat atggatacgg
     2281 cgttatttgg acaaacgctc cggaagtgcc cctgcatatc gggcgcatcg ggacgagcgt
     2341 ttcggcaagc cgtatccgaa tcccgttacc ggcgcggttt ggatacacgc cgtttcggtc
     2401 ggagaaacgc gtgccgccca gcccttgata cgcgagttgc ggcagcgttt tcccgatgcg
     2461 ccgctgctga tgacgcagat gaccccgacg ggacgggaaa ccgcgcaagt tctgtttccc
     2521 gatgcgcaat gccgctatct tccgtatgac aaaaaaacgt gggtacggca gtttttgcgc
     2581 gaacaccgcc cgatgttcgg cattttgatg gaaaccgaaa tctggcccaa tctgatgaag
     2641 gaatgccggc gcgcgggtgt gccgctgttt ttggcgaatg cgcggctgtc ggaaaaatcg
     2701 ctgaacggtt atctgaaagt ccgccgcctg atccgtcctg ccgccgcttc gctgacgggc
     2761 tgtctggcac agacggaggc ggatgcggcg cggttggcga aattgggcgc ggcatccgtg
     2821 caggtgtgcg gcaataccaa atacgacctg atgccgtcgg aacagatgaa aacgctggcg
     2881 gggcagtttg aaaaacgcat cggaggccgg ccggttgccg tgtgcggcag cacgcgcgtt
     2941 tatcggggcg aagacgaggc ggaaaaactg ctggcggcgt ggcaacaata tcgcggcgat
     3001 gcgctgctgg tcgtcgtgcc gcgccatccc gagcattttc agacggtatt tgaaacggca
     3061 aaacgcttcg ggtttaaggt tcagcggcgc agcgacggtt tgccggtcga acctgatacg
     3121 caggtgtgga taggcgacag tatgggcgag ctgtatgcgt attacctgtg cgccgatgtc
     3181 gcttttgtcg gcggcagtct ggtcgattcg ggctgtcaga acattatcga accgctttcc
     3241 tgcggcgttc cgacgatatt cggcttttca acctacaatt tttccgaagc ctgccggcac
     3301 gccttggcat cgggggcggc ggttcgggtt gagtcggcgg acgcgtggcg ggaagccgtt
     3361 gaaaaaacct tatcgtccga ggggggcgga atgcagatgc aggcgcacgt ggacggcttt
     3421 atcgcacaac atcgcggagc gggcgcgaga atcgccgagg cggtacggga agcggtatgc
     3481 ggacatcggg gacgatagtg atacaatccg tatccgagtt ttccggttgg agcatcgtat
     3541 gagtatttat gccgtcgcgc acatcgtcca cctgtattgc gccattgcct ttgtcggcgg
     3601 cgtgtttttt gaagtgctgg ttttgtccgt cctgcatacg ggacgggtgt cgtgcgaggc
     3661 gcggcgcgaa gtggaaaagg caatgtctta ccgcgccgtc agggtgatgc cgtttgtggt
     3721 cggactgctg ttcgccagcg gcgtcgtgat ggcggcaaac cgctatcttt ctatattggg
     3781 cgaaccgttt gccacttcct tcggtacgat gctgacgctg aaaatcctgt tggcgttcag
     3841 cgtgttggcg cacttcgcca tcgccgtcgt caaaatggcg cgttccacac tgaccgtcgg
     3901 ctggtcgaaa tacatacacg ccgtcgtctt tacccatatg ctgctgattg tctttttggc
     3961 aaaagcgatg ttttatatca gctggtaaca caattacatt tcaaatcagg agattgaacc
     4021 atgacagaat atcccggcat cgggtcgccg ttgttttacg gcgttttttt tgcggcagtg
     4081 ctggtcatga ttgccttgga tatgttttcg ctgaagaaaa acggcagcca taaagtcggc
     4141 gtcaaagaag ccttggcatg gagcggcttg tgggttgccg tatcctgcct gttcgcgggc
     4201 tggctgtatt tcgaactcgc cggcaatctc ggctacggcg cggcagtcgc aaaagaaaaa
     4261 gtattggaat tctttaccgg ctacattttg gaaaaatcgt tggcagtcga caatattttc
     4321 gtgttcctga tgatattcgg ctacttcaaa gtcgcaccac agtttcagca ccgcgtgctg
     4381 ctgtacggcg tattgggcgc attggtattg cgcactgtca tgattttcgt cggcgcggca
     4441 ctggttcagc agtttgagtg gattctgtat ctgtttggcg cgttcctgct ctatactggt
     4501 atacacatga tgaagcccga aggcgatgag aaagaggatt tggcaaacag caggctgctg
     4561 aatgccgtca agaaagtcgt tccggtcggc acggaatttc acggcgagaa attttttact
     4621 gtcgaaaacg gtaaaaaaat cgccacgccg ctgtttttgg tgctggtcat gattgaattg
     4681 agcgatgtcg tttttgccgt ggacagcatc cccgccgtct ttgccgttac caccgatccg
     4741 tttatcgtgc tgacttccaa tattttcgca attctgggct tgcgggcgat gtatttcctg
     4801 ctggcggatg tggcggaacg cttcgtattc ctgaaatacg gcttggcatt cgtgttgggt
     4861 tttatcggtg tgaaaatgct gataatgcat tgggtgcata tcccgatttc cgtttcgctg
     4921 tcggtcgtgt tcggcgcatt gggtgcatcg gtgttgactt cattggttta tacgaaaaaa
     4981 cagtccgata aataaaatgc cgtctcaacg ccgacaaaat ggttgacagc atcaggcttg
     5041 atgtatagaa tttcgagctt gtcgggtcgt tagctcagtc ggtagagcag cggactttta
     5101 atccgttggt cgagcgttcg aatcgctcac gacccaccag ataaaacaaa aaagctgagt
     5161 tttcaaaact cggctttttt tgttttccga caatccttgt tcggaaagcg cggcatttcg
     5221 tcaaattctt tggtgcggct tccgtatttt tcccgaaaat cccttataat tccgcctctg
     5281 caaccttcat cgggtgatgc tttatgcgtt gtcccgagta ttgaaatatt acaagtaccc
     5341 accaggaaag aacaaacgtg gacaaactga aaatctccgc aaatggcccg ctcaacgggg
     5401 aaataacggt ctcgggcgcg aaaaacgcgg cattgccgct gatgtgcgcg ggtttgctga
     5461 catcgggtac gttgcgcctg aaaaacgtcc ctatgctggc agatgtgaaa accacgcaaa
     5521 agctgcttca ggggatgggc gcgcgcgtcc tgactgacaa tatcagcgaa tttgaaatca
     5581 acggcggtac ggtaaacaat acctgcgccc cttacgaatt ggtacggacg atgcgggcct
     5641 cgattttggt gctggggcct actttggcac gtttcggcga ggcgcaagtc agcctgccgg
     5701 gcggctgcgc catcggttcg cgccccgtcg atcagcattt gaaaggcttg gaagcgatgg
     5761 gtgctgagat tgttatcgaa cacggttacg tcaaagccaa aggcaaactc aaaggtacgc
     5821 gcgtggcgat ggatgtcgtt accgtcggcg gcacggaaaa cctgctgatg gcggcgacgc
     5881 tggcggaagg tacgacggtt ttggaaaact gcgccattga gcctgaagtg gtcgatttgg
     5941 cggaatgcct ggtcaaaatg ggcgcgaaaa tcagcggcat cggtacgtcc acgatggttg
     6001 tggaaggcgc gggcgagcta cacggctgcg aacacagcgt cgtccccgac cggattgaag
     6061 cggggacgtt tctgtgcgcg gtggcgataa ccggcggcag ggtggttttg cggaatgccg
     6121 ccccgaaaac gatggaagtg gtgttggaca aactggttga ggcaggtgcg gtgattgagg
     6181 cgggcgacga ttggatcgcc atcgatatgc ggcagcgtcc gaaggcggtg gacatccgca
     6241 cggtcgtcca ccccggcttc cccacggata tgcaggcgca gtttatggca ttgaatgccg
     6301 tggcggaggg aagctgccgc gtggtggaaa cgatttttga aaaccgcttt atgcacgtcc
     6361 ccgagttgaa ccggatgggg gcgaacatta caaccgaggg caatacggca tttgtgcagg
     6421 gtgtggaacg gctttccggc gcagtcgtca aggcgacgga tttgcgtgcg tccgcaagcc
     6481 tcgttatcgc cggtttggcg gcgcgcggcg aaaccgtggt cgaacagatt taccacttgg
     6541 atcgcggtta tgaaaatatt gaaaaaaaac tcggcagcgt cggcgcgaaa atcgagcgcg
     6601 tatccggctg atcgggttgc gatgccgtct gaagccttca gacggcatca attgtttctg
     6661 tatcgcaaac actgattgaa aaggactgat tatggcattt ttaaaactga ccgaacaaaa
     6721 cgtgcagggc aaaaccgtcc tcatccgcgc cgatatgaac gtgccgttca aagacggcaa
     6781 aatcagcgac gacacccgta tccgcgcctc gctcgcgtcc atccaatact gcatggacaa
     6841 cggcgcatcc gttatcgtga tgacccactt gggccgcccg accgaaggcg agttccaccc
     6901 cgaagacgac gttgcgcccg ttgccgcgca tttgggcggc ctgttgggca aaaacgtgaa
     6961 agtattgaac gactggcgcg aaaacaaacc ggctttgaac gcgggcgatg tcgtgatgct
     7021 gcaaaacgtg cgtatcaaca aaggcgagaa gaaaaacgat ttggaactgg gcaaagccta
     7081 cgcttctttg tgcgacgtgt tcgtcaatga cgcgttcggc accgcgcacc gcgcccaagc
     7141 ctcgaccgaa gccgtcgccc aagccgctcc cgttgcctgc gccggcgtat tgatggcggg
     7201 cgaactcgac gctttgggca aagccttgaa acagcccgcg cgccctatgg ttgccatcgt
     7261 tgccggcagc aaagtgtcca ccaagctgac catcctcgaa tcgctggcgg acaaagtcga
     7321 ccaactcatc gtcggcggcg gtatcgccaa caccttcctg ttggctgaag gtaaagccat
     7381 cggcaaatct ttggcggaac acgatttagt ggaagaatcc aaaaaaatta tggcaaaaat
     7441 ggcggacaaa ggcggttctg tgccgctgct gaccgatgtc gtcgttgcca aagcctttgc
     7501 cgccgatgcc gaagcggtcg tgaaagacat tgccgacgtt gccgaagacg atatgatttt
     7561 ggacatcggc ccgaaatccg ccgccgagct tgccgaactg cttaaagccg caggcacggt
     7621 cgtttggaac gggccggtcg gcgtgttcga gttcgatcag ttcgcaggcg gtaccaaagt
     7681 attggctgaa gccatcgccc aaagcaaagc gttctcgatt gcgggcggcg gtgatacgct
     7741 ggcggcgatc gccaaattcg gcgttaccga ccaaatcggc tacatctcca ccggaggcgg
     7801 cgcgttcctt gagttcttgg aaggcaaaga gctgcctgcc gtagctgctt tggaaaaacg
     7861 cggcgcgtaa gcggtttgat ataaaaaatg ccgtctgaaa cgttgtgctg tttctgacgg
     7921 catttttgtg tggcgattat tgtgctttgg ctgcccattc cgccggagtg gcggcaaccg
     7981 aaatggaaag tgcgtgcagt tcgttacttt ccagttgggc tttgagtccg tctttaatca
     8041 ggcggtggcg cgcgaggcgt gccttgcctt caaattctga tgaaacgatg acggcgaaaa
     8101 aatggtgtcc gtcgccttct acttcgatgt gttcgcattt tgccacgcct tcaatcatgg
     8161 cttttacttg ttccgaggtc agcatggtag gttccttgtt ggaaaaatgc gccattatac
     8221 tggcaaattg aaatgccgtc tgaaaacggg ttcggacggc atttttgacg gtgttttatt
     8281 ttttggcttt gaagccgagc aggtgctggg tggtggcaag ccacgcgccg aatacgccca
     8341 acgcgatgac gaagccgaac accagcccga gttcgccagc gtagaagaac cgccagccga
     8401 tattaagtcc gtagggtttg aaaatggcat cgaccaatgg gcgcacggca gagagcagcc
     8461 aaccgcaaag ccccaagctg acggcggcgg aaaggatgct ctgccacatg gcttgataaa
     8521 ggaaggggcg gcggataaac gacgcgggcg cgcccaagag tttggtgatt tcgatttctt
     8581 cttttcggct gaggatttgc agtcggatgg tgttgtgcgc gacaaggacg aacgccatcc
     8641 ccagcgtcag ggaaagaaac cataagattt tacggatgaa ttcgttgatt tggtacagtg
     8701 tctgtaccca ttccgtatcc atgttggcgg attcgaccat aggcagtttg gtaatgtccc
     8761 ggtagattgc ctgcatttgg gcgggcgtgg ttgccgggtc gggggtaacg ataaagacat
     8821 ccggcagggg gttgccgtca agcatggaaa tcagattttg gtcgagattg gactgtaatt
     8881 cttccaaacc gtcttctttg ctgataaagc ggatgttgtc gagtcgtttg tcgcgcgcca
     8941 gcaggctgcg gacggtatcg ctgtcgcttt gtgcggcggc ggtttccata tagacggtga
     9001 tttgcggcga ctcattgagt ttgcccaaca cgctttgccc gctttggatg cccaagtaca
     9061 taaacagcgg cagggtcatg gcgacggcga gcattatgag ggtaagcagt gtgccgaaag
     9121 gttggcgcag aagctgtttg agcgcggtgc gcgcggattc gacgtgcagc gagaggtagt
     9181 ggatgatgct catgcgagtc gtcctttcga gaggcgcagg atgcggtgtc cgtagtccgc
     9241 catcagggtt tcgtcatgtg cggcgacgat gacggttgtt cccgcttcgt ggaaggtttt
     9301 gaacaattcc ataatatcga gcgcgtaggc gcggtcgagg ttggcggagg gttcgtcggc
     9361 aatcagcagg ctgggctggt gaacgacggc tcgggcaatg cacaggcgtt gttgttcacc
     9421 gcccgagagg gttacgggat cgtccaattc tcgccctttc aagccgactt tttcaatggc
     9481 gatgcgggcg cgttcttcgg ctttgcgcgg cggataaccg ataatccgaa gcggcaggat
     9541 gacgttttgc aggacgttgc ggtcgtagag gattttgtgg tcttggaaca cgatgccgat
     9601 gtgttggcgc ataaagccga tttggttgtc ggacaatgtg ccgaggtcct gcccgttaaa
     9661 caggattttg cccctgctcg gcttggtgat gcccgaaatc agcttgagga cggtggattt
     9721 gcccgaaccg gagtgtcctg cgataaatat catttcgcct ttgttgattt ggaagctgac
     9781 gtttttcagg gcttcaaaac cgccgggata ggttttggaa acttgttcga aacggatcat
     9841 aggaggtcct gtaaggaatg ggcggcatat cgtttcgggc ggttgcgtcc gcagggatat
     9901 gccgtctgaa caataggtaa gacagtgccg attataaaga ttccgcctgt ttttagatag
     9961 tggataagtt tggcggatac cgaaggtcgg gcgggaaaaa tgccgtctga agcggattca
    10021 gacggcatcc ggcgtttaac ggcattttga atgggcgagt ttgacggctt cggtcaggct
    10081 tttttcgttc acttccccgg taatggtctg cctgtatccg catttcggtg cttcgacgac
    10141 ggtaaagggc agtacgccga cagtgtttcc gtaggttttc ataaagtttc ggctgttcgc
    10201 cccagtgtaa cgccaaatcg ggtaggaaac aggagtttgt ttgaggaagt tgccgatatt
    10261 gtcggatgtg tcgagcgcga tgccgaccat atcgacgctg ccttttttct gcgctttgta
    10321 ccatttggac atggcaggca tctcttttcg gcacgggccg caccaggttg cccaaaggtt
    10381 gacgatgcgt acgggggctt tgagtgattg caggctttgt ggggtgttgt ctttccatcc
    10441 ggccagttcg tctgccgagg cggtatgtgc ggcaaatgtg gcggcaaggg tgattgcggc
    10501 aagattcaag tatttcatcg ttttgtcctt tgtatgtcga ttcgggtatt gtaaaccgtt
    10561 tggtacggtt gtaggcggga tattgagtaa aaccctgtgt aagccgataa taaggggatt
    10621 gtcgagaagt aaggaaatgt catgtctgaa atcaaaattt tccacaaccc ccgttgcagc
    10681 aaatcgcgtg ccgccttgtc cttattggaa gaacgcggca ttgctgccga agtggtcaaa
    10741 tatttggata cgccgcccga cttgtccgaa ttgaaggata ttttcaacaa attgggcttg
    10801 gagtcggcgc gcgggatgat gcgtgtgaaa gatgatttgt acaaggaatt gggtttggac
    10861 aaccctgatt tggacaatga cgcgctgctg cgtgccatcg ccgatcatcc cgccctgttg
    10921 gagcgtccga ttgttttggc gaacggtaag gcggctgtag gcaggccttt ggaaaatatc
    10981 gaagcggtat tgtgaaaaaa cggctgtttt gcagccgcaa cggcttgcgc tattacctgt
    11041 tgggcggttt ctgtttgagt gtttttccgc ttttgctggt gtttgcctca tccgtttggg
    11101 cggtttatcg gacgggcggt caggttttgc cgccgtatgt ccgggcggat gccgcgctgg
    11161 tgttgggcgc cgccgcgtgg gacaaacgcc cttccccggt ttttcgggaa cgcatcaacc
    11221 acgccatcgc gctttatcag agccgcaggg tcggcaaaat catttttacc ggcggcagga
    11281 gtaaaaaggg ttatatgacg gaggcggaag tcgggcggag atacgcgctc aagcaaggaa
    11341 ttccgccgcg caacatcctg tttgaaaaca cttcgcgcaa cacttatgaa aacctgaaca
    11401 acatccgtcc ggttttgcgt gccaacggta ttgccagcgt ggtgattgtc agcgatccct
    11461 accatttggc gcgggcggcg gaaatggcgg aggatttggg tgtccgcgtt tatatgtctg
    11521 ccaccccgac cacgcggttt gatgcgggaa ataagaaaaa aatctttatg ctgcaagagg
    11581 gatatgcgtt atccctctac cgtttggaaa aatgggggcg ggattttttc aattggctgt
    11641 ttcattgaac ctttttttcg gacgcaatcc cgaaggaacc cggtcggcgg cgcgtgatac
    11701 actgttccgc gtgccgaagc attgaaaaca aggaaaatgc cgtctgaaac ttatccgcag
    11761 gcagggttcg gacggcattt ttaatgccca agcacggtaa aaacagtaaa ataagccgtt
    11821 ttaaagattc tggagccatc atgaccgtca aaacccgttt cgcccccagc cccaccggct
    11881 acctgcacat tggcggcgtt cgcaccgcct tgttttcatg ggcgtttgcc cgccatcaca
    11941 aaggcgagtt cctattgcgt atcgaagaca ccgacttggc gcgttccacc gccgaatccg
    12001 tcaacatcat cctcgacggc atgaaatggg tcggtctcga ttacgataac gccgacaacg
    12061 tggtgtacca aacccgccgc ttcgaacgct ataaagaagt catcgccgaa ctcttggcaa
    12121 aaggcgatgc ctactactgc tattgcagca aagaagaatt ggaagcgatg cgcgagaaag
    12181 ccgaaaagga aggcacggcg acttacgacc gccgctggcg gccggaagca ggcaaaaccc
    12241 tgcccgaaat ccctgccggc gtgcaacccg tcgtccgctt caaaacgcct ttggacggcg
    12301 taaccaagtg ggcggacctg gtcaaaggcg aaatttccat ccccaacgaa gccctcgacg
    12361 acctgattat tgcacgcgcc gacggcacac cgacctacaa cttctgcgtt gtcgtagacg
    12421 actacgacat gggcgttacc cacgttatcc gtggtgacga ccatgtgaac aacaccccga
    12481 aacaaatcaa catattaaaa gccatcggcg ctaccctgcc tgaatacggg cacctgccga
    12541 tgattctcaa cgaacaaggc aaaaaaatct ccaaacgcag cggcgatacc gttgccatta
    12601 ccgatttcgg cgcaatgggc atcctgcccg aagccatgct caactatctg gcacgtttgg
    12661 gctgggcgca cggcgacgac gagttcttta cgatggaaca attcatcgaa tggtttgatt
    12721 tgaaagacgt ttccccgtcc ccaagccgta tggatttgaa aaaactctat tggatcaacg
    12781 gcgaacacat caaaatcacg cctaacggca aactcgccga actcgtcaaa ccgcgtctcg
    12841 ctctgcgcga tatccatgaa actgaaaaac ctgcgttgga agacgtgttg gcacaggtca
    12901 aagaccgcgc ccaagatttg aacgccttgg cagacgaatg tctgtatttc tacaaaaaac
    12961 aagtccctgc cgaagccgat gttgccaaac actgggatga cgaagcggcg gcacgcatgc
    13021 tgcgctttgc cgaacgcctc gagggtttgg aagactggaa taccgaagcc atccacgacc
    13081 tgttcaaacc tttctgtgac gaagaaggca ttaaaatggg caaactcggc atgcctttgc
    13141 gcctcgctgt ctgcggcacg gcgaagaccc caagcgtcga tgccgtgttg gcattaatcg
    13201 gcaaagagga agtgttgaaa cgcatccgcg cttaagttat ccacaggagt gggcgatgcc
    13261 gtctgatgtc ggaatacggc ttcagacggc atttaaacgg aagtttaaaa tgaaaaaaat
    13321 attttatttt ctgatggttg ttttttctac aagcgtatgg gccggggatg cggaagacaa
    13381 tctgctcagc atccaatccg gctatcgtgc cttactgcaa aagcaaaaca atctggacgg
    13441 aaaaatcatc gggatgcagt cggatttgga agatgcgcgc cggcgtttgc aggcggcaca
    13501 ggcggatatt aaccgtttgg aagcggaaat tcctgcagca atggcgcaaa aagcccggca
    13561 ggctgaagag ctgaggcaaa tcggagtgcg tttggaccat gcttggaatg cggtttacgg
    13621 cgcaggggga acgaaggcgt cggggaattg accgatgaat tccctctttg tggacaatac
    13681 tgttttcatt acacggctga aagccgggca tatcggcagg ttggttcagg cgttgtttga
    13741 ggagtggcac ggatttgaac cgtggtcttc tgtggataag attcgtgcct attacggcag
    13801 atgtttgaaa gatgacgaac tgccgctggc ttttgcggct gtggatgatt tcggaaccct
    13861 gttgggttcg gctgcggtca agcggcatga tatggaaagt tttccacagt atgaatattg
    13921 gttgggtgat gtctttgttt tacctgaata tcgcggaaaa ggcattggca ggaggctggt
    13981 cgcccactgc ataggcgcag cgcgttcgct ggggataaag ttcttgtatc tttatacgcc
    14041 tgatgtgcaa atattttatg aatcattcgg ctgggcggtt gtcgggcgac atttccataa
    14101 cggcgaatgg gttacggtta tgcgtttgga tatggataag gtttaagccg aatggacaaa
    14161 tgccgtctga gctgttcgga cggcattttt tatgtttgga aacttcctgt cagataatat
    14221 ttatccacag atttttccat atggtttgat gctgttccga caacggaaat taagatacgg
    14281 atttgcacaa atctttcaga cggtacagtt ctgacaatgc ttcgcgcggg gtcaggctgt
    14341 cgggatcgag tttttccaag gctgctgcca atataccttc aaaatgtttt tcctctgctt
    14401 tatccacaaa gttgcccaca ttcggttcat ctcctttttc agacggcatg gtactgaaaa
    14461 tatccagttg gggacggttc gcggcggctt ggttttccag tccgttcaaa tgcttttggg
    14521 cggatttcaa tgcgcgtaca ggcaggccgg cgagtttggc gacggcaatg ccgtagcttt
    14581 taccggcggg acccggttgg atttggtgca ggaaaacgat gtcctgtccc tgttcgagcg
    14641 cggaaaggtg catattgacg gcggcggcgt gggcttcggg caggtaggtc agctcgaaat
    14701 agtgggtagc aaacaggctg aaggatttgt ttttttgcag caggtgttcg gcaacggcgt
    14761 gcgcgagggc gaggccgtcg aaagtggaag taccacgtcc gacttcgtcc attaaaacaa
    14821 ggctttgttc ggtggcgtga tgcaggatgt aggcggtttc gctcatttcg accatgaaag
    14881 tggagcggtt ggaggcgagg tcgtccgatg cgccgatgcg ggtgaagatt tgatcgatgg
    14941 gcccgattgt ggcggcatcg gcaggcacaa aacagccggt gtgtgccaat aaaacaatca
    15001 gcgcgacttg gcgcatgtag gtggatttgc cgcccatatt ggggccggtg agcagcatga
    15061 ggcggtgttt gtggtcaagg tcggtgtggt tggcggtgaa gtggcgtacc tgctgttcga
    15121 caacgggatg gcggccgttt tcgatgtgga taaccggata gtcggcaaac tcggggcgga
    15181 cgaagttccg ctcttttgcc aaggctgaaa atgtggacaa cacgtccagc gcggcggcgg
    15241 ctttggcggc tttttgaagc tgcggcaatg ccgtctgaag gtttttcaat acgccgtcaa
    15301 agagttgttt ttctaaggcg agggcttgct cttgagcagt cagcactttg tcttcaaagg
    15361 ctttcaactc cggcgtgatg aagcgttcgg cgtttttgag ggtttgccgg cgttggtagt
    15421 cggcaggtgc ttgttcggct tgggttttgg acaattcaat gtaaaagccg tgaacgcggt
    15481 tgaactcgac tttgagtgtg gacaaaccgg tacgttcgcg ttccttggct tccaaatcca
    15541 gcaaaaattc gtcgccatgg ttttgaatgc ggcgcaattc gtccagttcg ggatgaaaac
    15601 cgtggttgat gacattgccg tctttcagcc agacggaagg ttcgggcaaa atggcttggc
    15661 ggagctgttc ggctgtggat aggttttccg ggaaaacggc tttgagggtt cctaagagac
    15721 tgctgcactc ggcggacaat tcgatttcgg acagggcaaa caggctgtcg cgcagggcgg
    15781 cgaggtcgcg cgggcgggcg ttacccacgg caatacgggc ggcgatgcgt tcgatgtcgg
    15841 caatgctttt cagacggcac tggaggggtt tgtattggct ttccagcgcg gcaacggctt
    15901 cttggcgcgc tcggatgtgg gcgcggttgc gtaaagggtg gtgcagccag agagccaaga
    15961 ggcggctgcc catatgggta gcgcaaaggt cgagcgtgga catcagggtc ggcgattttt
    16021 tgccggagag ggtttgcgtg atttcgagat tgcggcgcgt ggcggcatcc ataccgatat
    16081 attggctgtc ggtttcgagc gacaggccgt ccaaatgttg cggcatcagg ttttgcgtca
    16141 gacggatata gttcaacagt gcacctgccg cgccaatcgc aacggcgtgt tctttgccgt
    16201 ccaaaccgaa gccgcgcaaa tcctggcagc cgaaatattc cgtcagcagt ttttcccccg
    16261 cgtcggcggc aaactgccac gcgttcaggc gcgtaacacc cgatgccgtc tgaagttgcg
    16321 gtgcgttttt actgtccggc aacagaattt ccgccgcctg caggcgcgcc agttcgtcgt
    16381 ccaatttatc cacagttgtc agcttggttt tgaattcgcc gctttgcagc gatgcccacg
    16441 ccaaaccgat gtattttttg tcgggggaca cggcaacgat gcggttggtt tccttgtctt
    16501 ccagcaatgc ggaatcggtc agcgtgccgg gcgttacgat gcgcacgact ttgcgctcca
    16561 ccggtccttt gcccgcgccg acttcgccga cctgttcgca aatcgccacg cttttgccca
    16621 acttgaccag gcgcgccaga tattgttcgg cggcgtgaaa cggcacgcct gccattttga
    16681 ccggctcgcc atccacctgt ccgcgcgtgg tcagggtaat atccaaaagt tttgccgctt
    16741 ctaccgcatc gtcgaaaaac atctcgtaaa aatcacccat gcggtaaaac accagtttgt
    16801 cggtatgttg cgctttgatg ccgaggtatt gctgcatcat tggggaaacg gcggatttgg
    16861 acatggggct gccttatcat taggaaaacg gtattgtagc aaaaggtttt gtcttcgacg
    16921 gaatgagact gaccgtaagc ctgtcaataa ggttgtggag acaagtagga aaacagcaaa
    16981 gttatccacg gagatattat taagtcaaaa aacatccacg cttatctgtc ttatacagca
    17041 agtcttaaac tcaaacttat ttgacaaaaa gataatgaaa atattggata tttgctactt
    17101 acgcacaagc aggttggcaa tcatcaaaat catcattaaa atttaaattc aaatattgat
    17161 ggtaaaggtt ttggaaatct gtgcaaacag caaaattcat ctatatcaaa taagcgtcaa
    17221 gtagttagat gtaatagtgg aatgtaactt tatttcagaa tacaatgcag gcatcggact
    17281 ttatttcaca acctggagac aaaatatgag cattaaagta gcgattaacg gtttcggccg
    17341 catcggccgc ctcgcattgc gtcaaattga aaaagcccac gacatcgaag tcgtcgccgt
    17401 caacgacctg acccctgccg aaatgctgct gcaccttttc aaatacgaca gcacgcaagg
    17461 ccgtttccaa ggtactgccg aattaaaaga cgatgccatc gtggtaaacg gcaaagaaat
    17521 caaagttttt gccaatccga atcccgaaga attgccttgg ggcgagttgg gtgtggatgt
    17581 cgtcctcgaa tgtaccggct tctttaccaa caaaaccaaa gccgaagccc acatccgtgc
    17641 cggcgcgcgc aaagtcgtga tttccgcacc tggcggcaat gatgtgaaaa ccgtcgtata
    17701 tggcgtgaac caaaacattt tggacggcag cgaaaccgtc atttccgccg cttcctgcac
    17761 caccaactgc cttgccccga tggcggcagt cctgcaaaaa gagttcggcg tagtcgaagg
    17821 tctgatgacc accatccacg cctacaccgg cgaccaaaac acccttgacg cgccgcaccg
    17881 caaaggcgat ttgcgccgcg cccgagccgc cgcgctcaac atcgtgccca acagcaccgg
    17941 tgccgccaaa gccatcggtc tggttatccc cgaattgaac ggcaaactcg acggctccgc
    18001 ccaacgcgtc cctgttgcca ccggctcgct gaccgaattg gtttccgtcc tcgaacgccc
    18061 tgtaaccaaa gaagaaatca acgccgcgat gaaagcagcc gccagcgaat cttacggcta
    18121 caacgaagat caaatcgttt cttccgacgt tgtcggcatc gaatacggct cgctcttcga
    18181 tgcgacccaa acccgcgtga tgaccgtggg cggcaaacaa ttggtgaaaa ccgttgcctg
    18241 gtacgacaac gaaatgtcct atacctgcca actcgtccgc actttggaat actttgcagg
    18301 caaaatctaa ggcttgaaca aacctgtgga taagtaaatg ccgtctgaac acattgcgtt
    18361 cagacggcat tgtttatctg tatagtggat taaatttaaa ccagtacggc gttacctcgc
    18421 cttgccgtac tatttgtact gtctgcggct tcgttgcctt gtcctgattt aaatttaatc
    18481 cgctataact tgatattgcg gcttatggcg aacaaacaac agccgtttgt tcaactgcta
    18541 tgcggcaaaa ccggattttt gcccgattta aagcggggtt ggggaaagat caataacggt
    18601 tgcacggttt tcctcgattt gaaatctgac ttcgtaatct gcaatattca tcacataaat
    18661 ccgttcggga atattctgat aggcggggcg cggatcttgg gcaatgcttt ggctgataag
    18721 gtttttggtg tttgcagata aattttccgc gccgatgttt tcctgccaaa cgacttccaa
    18781 ctctacgggt ttgccgctga cgaaaccaga tgcggcatcg ggtttggatt cgacaaaggg
    18841 gatataaggc ttgatgtcca caatcggcgt gccgtccagc aggtctgcgc cgctgcaata
    18901 gaggcggacg ggtttgccgg tttcgatgcg ttcgagtttc aggagcgaga gtccgagatg
    18961 gttggggcgg tgggggctgc gcgtggcgaa cacgcccatt ttttgtttgc cgccgagccg
    19021 tggtgggcgc accatttgcg cccagccttc atccaatacg ccgtgaaaaa taaaacttat
    19081 ccacacatag tcgaagtgtt ccagtccgcg tacgctgtct gcggtaaatt cgggattcag
    19141 ctcgatgcag gcttttgcgg cggagaccaa accgggctgg cgggcgatgc cgaatttctg
    19201 tttgtagggc gagcgggcgg tgccgatggg ggtaatggta taagtcataa ggttgtgggt
    19261 aagttttttg gaaaaacctt atcttatcac aatcggtttt accgcttttg ggcagtaatt
    19321 tgttataata cagggcattt taatcgcgtt gaaataatga gatgattgtt tccattgatg
    19381 ttgatgcaca aaaaacgttt acgccactgt gtcctgacga actgcccgtg aacgaggggc
    19441 atttgattgt cgaggagttg aatgctcaag ccgctttggc ggatttgcgc gtgatgacga
    19501 aagatgcgca tcatatggta gcgaaatggc ttgtggataa tcctgttgat atgttgaagc
    19561 cgacaggttt gcctgatgcg gatttgactt gggtggctca tgcgatggtc ggtacgcgcg
    19621 gctatgaatt attggacgga ctgccttctg ccaaagaata cgattattgc gtttggaaag
    19681 gtgttgatcc tgaattgcat ccttacggcg cgtgttttca cgatattgag gaaaagctga
    19741 gcacagggct gattgaatgg ctgcgttgtc aaaatacgga tacggttatt atcggtgggt
    19801 tagctacgga ttattgtgtt aaaacaacgg ttttgcagtt actcaaaggc ggtcgttggc
    19861 aggttgtcgt caatgaagcg gcttgtcggg gtattgcgcc ggaaaccatc gaaacagcat
    19921 ggcaggaaat gcattctgcc ggggcaatca tcttaaaaaa cgctgatgaa ataaataaat
    19981 atattaataa tcaataagtt aaaattgttt cacgtgaaac acccttccta aatatgaaaa
    20041 ttttgcttgt ccgcttgtct agtatgggcg atttaattca cactttgccc gcaatcgaag
    20101 atttggcgcg acaatgtcct gatgtggaac tgcattggct atgtgaggct ggatttgcag
    20161 atattgcgcg cctgcatccg tttgtaaaaa aaatccatgt gatgaaatgg cggcaatggc
    20221 gcaaacatct ctttcgggct gaaacttggc gggaaatggg tcatctgaaa cagactttgc
    20281 ggcaggaagt atttgatttc gtattggaca gtcaaggtct gattaaaagc gcgtgtttcg
    20341 ctaaagtggc aaaatccccg atttgtggtt tggataaaaa cagtgcgcgc gagggatggg
    20401 ctgctttggc gtatgtaaaa acatacgctg taccgaaggg aaaaaatgcc gtttggcgca
    20461 accgtgaact gtttgcccaa gtatttgggt atgtaatgcc ggaaacgcag gtatttggct
    20521 tgactgttcc tgaagcaggc cgtctgaaaa atttagcgca gccgtattat gcggctttgc
    20581 atgcgaccag tcgggacagt aagttatggc ctgtggaaaa ctggcggtcg ttgctgcaaa
    20641 agttgaatga agaacagcaa tgcaatattt acctgccttg gggaaatgaa gatgaaaaaa
    20701 cgcgtgccaa acaaattgca gatgggctgc cgtttgctat tgtgtgcgac aaaataaatt
    20761 tattgcaggc agcgtatctg ctgaaatacg cggtcggaat tgtcggcgtg gataccggtt
    20821 tgctgcattt ggcaaatgcc ttggaaaaac ctgtggtcgg catttatacc gataccgatc
    20881 cgattaaaac aggcgttcag gtttcgccaa ttgcaaaaaa tttgggcaat atcgggcaga
    20941 ttccgaccgc agatttggtt tatcaagcgc tgatggattg tgttgcagca gataaaggct
    21001 aaagggtcgt ctaaaactga tatggcggca tgatattttc caatttatta gatacaggaa
    21061 tgtatatccg ctagtctgag taaatgcttt aaaaatggta taatcggcaa gcattatccg
    21121 aaagattttt gaatgaattc gtttttaagg aaaacggatt tggttttata gggaagaagg
    21181 tttggtttcg ctggatggtg aaagcagcgt tctttttagg agggttcaat gaaagcactg
    21241 gtcgcagtaa agcgcgtagt ggactacaac gtcaaagttc gtgtaaaagc cgatggttcg
    21301 gatgtggata tcggcaatgt caaaatgtcg atgaatccgt ttgacgaaat cgctgtggaa
    21361 gaagccgtcc gtttgaaaga agccggaaaa gtaagcgaga tcgtagcggt ttctttgggc
    21421 gaaaagaaat gcgaagaaac gttgcgtaca gctttggcga tgggtgccga ccgtgccatt
    21481 catgttgaaa ccgatactaa actggagtct ctggccgttg ctaagttgct gaaagccgtt
    21541 gcggacaaag aaaatccgca aattttcttt ttgggcaaac aagcgattga tgatgatgcc
    21601 aatcaagtgg cgcaaatgct ggcagctttg ctgaatgcgg cgcaaggtac gtttgcttca
    21661 aaagtacaca ttgaaggtgg tgaagtacag attgtgcgcg aaattgatgg cggcgaagaa
    21721 accatcgcat tgaaactgcc tgctgttatc agcgcggatt tgcgtttgaa cgagccgcgc
    21781 tttgtcaaac tccccaatat tatggtggca aagaaaaaac ctttggaaaa actgactcct
    21841 gacgatttgg ttgccgacat ttcacctcgt ttgaaaacgg tgaaatttgc cgaacctaag
    21901 gcgcgtcagg caggcgtaaa agtagcaagc gttgccgaat tggttgaaaa attgaaaaac
    21961 gaagccaaag tgatttgagg aggctgaaat gagtgtattg attattgtcg aacacgacaa
    22021 caaacagttg aatcctacca ctttgcatgc tgttaccgct gccgctaagc tgggtaaaat
    22081 cgatttattg gttgccggaa gcggtgcatc tgctgtggta gagttcgcaa aacaagtggc
    22141 gggtgtggaa aaagttttgg ttgcagatgc tgcccattat gccgaaggtt tggctgaaga
    22201 gttggctccg ctggttgtta aattggcagc ggattactgc tatgttgcag caacggcaac
    22261 tacattcggt aaaaaccttt tgcctcgcgt agcagcctta ttagacgtac cgcaaatttc
    22321 tgatttgacc gaaattgtgg ataacacgac ttttgtgcgc cccatttatg cgggtaatgc
    22381 atttgaaacc gtgcaagctg attcagaaaa attggtgctg accttccgtg cgacggtttt
    22441 tgacgcagtg gcggcgcaag gtggtaatgc tgaggtaatt aatgttgaag caacccctgc
    22501 ccaaaacctg agtcgttttg ttaaccgtca gctttcccat tccgatcgtc ctgaactgac
    22561 tcaggcaaaa gtgattgttt ccggtggtcg tgcgttgggt agtgcggaaa aattcaatga
    22621 agtgctgaca ccgttggcgg atgttttagg tgcggcaatc ggtgcatccc gtgcggcggt
    22681 tgatgccgaa tatgcgccaa acgatgctca agtcggacaa accggtaaag tagttgcgcc
    22741 gcaactctat tttgcaatcg gtatttcagg tgcgattcaa cacgttgccg gtatgcaaga
    22801 cagtaaggtg attgttgcaa tcaataaaga tgctgatgcg ccgattttca atgtagccga
    22861 ttacggattg gttggcgatt tatttgaaat cgttccacaa ttaacagaag tattgaaaaa
    22921 ttaatgtttc acgtgaaacg ttcagacgac cttgttatta atgaggtcgt ctgaaaacat
    22981 ttcagcatga ttacgactta taaaaccatt acttcctcga cgcaggctga gtttaaagat
    23041 aaaggcagcc gttttattgc atttgcctat ccgattcgga cattggctga tgtgaaaaaa
    23101 tacctcgatc cgttaaagga agagcatcat aaagcacgac actggtgcta tgcctatcgt
    23161 ttgggtgtgg atggcatgca atttcgtgcc aacgatgatg gagagccatc aggaagtgct
    23221 ggacgaccaa ttttgggaca gattgattcg gtgggtatta ccgacgtttt ggttgtggtc
    23281 gtccgctatt tcggtggtac tttgttaggt gttccaggtc tgatacatgc gtacaaagag
    23341 gcgacggctc aagcgttggc agttgcagaa gtagttgaaa agaatattga aaaaactgtt
    23401 tggctgaaat gtgaatatcc gtttttgaat gaagcgatac gcattgctaa acaatatcag
    23461 gcggatgtat tggagcggga tttgcagttg gattgccgat tgacggtcag tttgtcgttg
    23521 gtaaattatg aggcctgtgt tgcggcttgg aagagtacac ggcagattga agtaaatacg
    23581 gaaaagcctt ttgaatgaaa ggtcgtctga aatgctttgt gatggtattt cagacgacct
    23641 cttcgctgac taatcttctt gtgtatattt gtctaaggca tcgacgctgg agcgcaaata
    23701 taaccaagcc agttgatgga attcgcccag cgcgttccat agtgcatctt cactcataat
    23761 gctgtattcg ccttctgtgc gcaaatccac ctccgctcca tcttcaatag cccgatgaca
    23821 agcctgatat tcgtgggtat aaaaattgtc catatcgcga atcaaagcga cggcatgttt
    23881 ccaacggcgg atgtcttctt ccaattgcgg tagtaaatga caccattcgc ggtatttgct
    23941 ttggatttca tcaatacgtt tttgcatggt tgcttgtcct tttttaattt atgaataagt
    24001 tgaataggtt gcagagtgga atgaagcggt cttttgtttg attctactaa aatttcttca
    24061 gatgggataa aagtcccttt gtatcgagtt ttcgttaaaa gtacatattt gtcaaggaga
    24121 tggcatgaaa ctgctggtta tcggtaatgg cggtcgcgaa cacgcgctgg cttggaaatt
    24181 ggcgcagtcg cctaaagtgg aaacagtatt tgttgcgtct ggtaatgccg gtacagcgat
    24241 tgaacccaag ctgcaaaaca tcgacttgac tgcgcatcag gatttgattg aattctgtcg
    24301 taaagaaaat attgttttta ccgtcgtcgg ccctgaagcg cctttggcgg caggtgttgt
    24361 ggatgatttc cgtgctgcag gactaaaaat attcggtccg acacaatatg cggcgcagtt
    24421 ggaaagttcc aaagatttcg ccaaagcatt tatggcgaaa tacaatattc cgaccgcgca
    24481 atatcaaacc tttgaaaacg ccgatgccgc acatgattac gtcaatcaga aaggtgcgcc
    24541 tatcgttatc aaagccgatg gtttggcggc aggtaaaggc gtgattgtgg cgatgacttt
    24601 ggatgaagcg catgctgcga ttgatgatat gctgctggac aacaaaatgg gcaatgccgg
    24661 cgcgcgtatt gtgattgaag atttcctgca aggcgaagaa gcgagtttta tcgtcatggt
    24721 tgatggcaat aatgtactgc ctatggcaac cagccaagac cacaagcgtc ttttagacgg
    24781 cgacaaaggt ctgaatacgg gcggtatggg tgcgtacagc cctgcgcctg tggtaacgcc
    24841 tgttgtgtac gaacgcgcga tgaatgaaat tattttgccg accgtagcag ggatgaaagc
    24901 ggaggggcat gagttcaccg gtttcctgta cgcaggtttg atgattgatc aaagcggtgc
    24961 accccatacg attgagttta actgccgttt cggcgatcct gaaacccagc cgattatgag
    25021 ccgactgaac agcgacttat cggatttggt tgaagcggca atagacggca aactcgatag
    25081 cgtaaccgcg gaatggagcc cgcaaactgc cgtgggcgtg gtactggcgg cgcaaaatta
    25141 ccccgaaacg cccaaaaaag gcgatgttat ttccggtctg gatgctgcca accaagtagg
    25201 caaagttttc catgcaggta caacggcaaa cgagaaaggt gacgtattga ccaacggcgg
    25261 acgcgtattg tgtgttgtgg gattgggcga taatgtggcg caagcgaaag ctaaggcata
    25321 tggcgcgttg gaaaaaatca gtttcgacgg gatgcaatac cgtaaagaca ttgccgataa
    25381 agcgattaac cgttaattag aaagaggatg atgccgtctg aagccaagta tgactgatgt
    25441 gcttcagacg gtattttata ttatgttatt tccgagaatt atcgcagcat cagcgcaacg
    25501 taagttaagc gtttatttga aaaaaggcgg tttggtcgct tatccgaccg aatcttgtta
    25561 tggcttgggg tgtcttccga cattagccaa agcactcggt aagttggcgc atttgaaaaa
    25621 aagaccgcaa cacaagggta tgattgtcat cgggaatcaa tttgagcaat tgcagccttt
    25681 acttcaaatg ccgtctgaaa atcttcagga tatgcttaga aaagaatggc ccgcccctaa
    25741 aacgttccta ttatccgcca aatcctgtgt cttacccgag ttacgcggga aacagcgcag
    25801 caagttggcg gtcagggttc ccgcccatgt aggtgcgcgc cgcttgtgtc aggctttgca
    25861 aacgcctttg gtctcgactt cgtgcaaccg cgcaggcaaa cgcgcgtgtc ggacggagag
    25921 ggaagtcaga aggcaattcg gacgcgatgt gtggattgtt ggtgggagaa tcggcaggca
    25981 gaaatcgccg agtcagatta ttgacgggga aacaggtaaa cgtttgcgtt agatatgggt
    26041 acttgaatga aaactttttt tgcatacaca aagaaaaaat ttttcggata gcatttctct
    26101 acatgattgt tattgcacag agataaaggt agaaggacgc aacgtctgct ttaattttga
    26161 ggacggtttt gtcgttccgg acaataatcc gaacaatcaa gcgggcaagc atctgaaaac
    26221 agatttttcc cgtgttgttt taacccataa aaatgaaaac gccgaagatg attatttgtt
    26281 cgatattttt gaagacatcg tttttttagg gaaacgtttg tttacggtca gaaaatttct
    26341 tgatttttct gaattgcttg agatggtcaa tacacaaggt tattttcttg agtttctgt
//
LOCUS       DAXHSF010000009        26174 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.009, whole genome shotgun sequence.
ACCESSION   DAXHSF010000009 DAXHSF010000000
VERSION     DAXHSF010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26174
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.009"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(225..548)
                     /locus_tag="ACFM8S_000290"
     CDS             complement(225..548)
                     /locus_tag="ACFM8S_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFC6313421.1"
                     /translation="MDIVQLGSAELKAWMDEGRMFCLLDVRTDEETAVCSLPNALHIP
                     MNLIPLRQNELPDDVPLVVYCHHGIRSLHTAMYLADAGFENLYNLQGGIDAWAAEIDG
                     NMMRY"
     gene            complement(627..1283)
                     /locus_tag="ACFM8S_000291"
     CDS             complement(627..1283)
                     /locus_tag="ACFM8S_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFC6313422.1"
                     /translation="MDFEKARFNMVEQQIRPWDVLDFDVLDALAEIPRELFVDEDLQG
                     LAYADMALPLANGHKMLEPKVVARLAQGLKLTKNDTVLEIGTGSGYATALLAKLASRV
                     VSDDIDAEQQNRAKAVLDGLSLENIDYVQNNGLTELSAGAPFDAVYVGGAVNLVPEVL
                     KEQLKDGGRMVVIVGRRPVQRALLITRRGDVFEEKVLFDTLVAHLDDKDADPFGGFQF
                     "
     gene            complement(1386..1889)
                     /locus_tag="ACFM8S_000292"
     CDS             complement(1386..1889)
                     /locus_tag="ACFM8S_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFC6313423.1"
                     /translation="MHALHFSASDKAALYREVLPQIESVVADETDWVANLANTAAVLK
                     EAFGWFWVGFYLVDTRSDELVLAPFQGPLACTRIPFGRGVCGQAWAKGGTVVVGDVDA
                     HPDHIACSSLSRSEIVVPLFSDGRCIGVLDADSEHLAQFDETDALYLGELAKILEKRF
                     EASRQAV"
     gene            2073..2846
                     /gene="tpiA"
                     /locus_tag="ACFM8S_000293"
     CDS             2073..2846
                     /gene="tpiA"
                     /locus_tag="ACFM8S_000293"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFC6313424.1"
                     /translation="MYHQIGMWDQKWVIGNWKMNGRLQNNNSLMHRFRILPTAERVLI
                     GLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPDNGAYTGEVSAEMLADTGTDIVL
                     IGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEEREAGKEHEVIAHQLSIL
                     QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSV
                     KADNAADIFAVPYVDGALVGGASLSYDSFTAIISAAQNA"
     gene            2853..3203
                     /gene="secG"
                     /locus_tag="ACFM8S_000294"
     CDS             2853..3203
                     /gene="secG"
                     /locus_tag="ACFM8S_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFC6313425.1"
                     /translation="MEAFKTLIWIVNIISALAVIVLVLLQHGKGADAGATFGSGSGSA
                     QGVFGSAGNANFLSRSTAVAATFFFATCMAMVYIHTHTTKHGLDFSNVQQTQQAPKPV
                     SNTEPSAPVPQQQK"
     gene            3217..3302
                     /locus_tag="ACFM8S_000295"
     tRNA            3217..3302
                     /locus_tag="ACFM8S_000295"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3251..3253,aa:Leu,seq:gag)
     gene            4215..4583
                     /locus_tag="ACFM8S_000296"
     CDS             4215..4583
                     /locus_tag="ACFM8S_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFC6313426.1"
                     /translation="MRIFKNQWIVKFAKKHKISDSELLEAVERADNGLIDADLGGGVI
                     KQRIARQGQGRSGGYRSLILFKQADRAFFVYAFAKNDRENISDKELDVYRKAAAYYLK
                     YTRAELAALKEDGIITEIES"
     gene            4580..4891
                     /locus_tag="ACFM8S_000297"
     CDS             4580..4891
                     /locus_tag="ACFM8S_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC6313427.1"
                     /translation="MKYKSEALAAIHEMMEGAYNIGAIDKKTMRGFDKSCLTEIKPLS
                     GGDIKAIREKEALSQAAFAIYLNVGKNHVSAWERGVKKPSGAALKLLTIVKNKGIEAI
                     A"
     gene            5001..5213
                     /locus_tag="ACFM8S_000298"
     CDS             5001..5213
                     /locus_tag="ACFM8S_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313428.1"
                     /translation="MPDRIKPSRNGGGADGIARQQGTAQTGAKPETSPTARHGDFFAP
                     VAGGIGFKRRGLEGKTGGAQNCLNAV"
     gene            complement(5274..6008)
                     /locus_tag="ACFM8S_000299"
     CDS             complement(5274..6008)
                     /locus_tag="ACFM8S_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFC6313429.1"
                     /translation="MNAAAAKDYMKTVELNKSAGNVDTTSRTARRVQAVFRRMLPYAD
                     AANNTGHKFDWKMTVFKNDELNAWAMPGGKMAFYTGIVDKLKLTDDEIAAIMGHEMTH
                     ALHEHGKNKVGQQILTNTAAQIGTQIILDKKPDTNPELVGLGMDILGTYGITFPYSRS
                     LEEEADEGGMMLMAQAGYHPAAAVRVWEKMNQENDQNGFIYAITSTHPTNNARIENLK
                     RLLPTVMPVYEQSVRNKGRVNKKRRR"
     gene            complement(6231..6344)
                     /locus_tag="ACFM8S_000300"
     CDS             complement(6231..6344)
                     /locus_tag="ACFM8S_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl-tRNA synthetase"
                     /protein_id="HFC6313430.1"
                     /translation="MRDSDKPAASEGAVKFIEGNPAKKIIAVPGRLVNIVV"
     gene            complement(6337..6507)
                     /locus_tag="ACFM8S_000301"
                     /pseudo
     CDS             complement(6337..6507)
                     /locus_tag="ACFM8S_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
     gene            complement(6474..7265)
                     /locus_tag="ACFM8S_000302"
     CDS             complement(6474..7265)
                     /locus_tag="ACFM8S_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DpnI domain-containing protein"
                     /protein_id="HFC6313431.1"
                     /translation="MNLFFDTELGKQQNKATHKIRVMSEAWLEKNGYCPCCGSKPMQR
                     FANNKPVADLFCPNCHEQYELKSKNQKTIGNSVPDGAYRTMLERIRSDTNPNFFFLAY
                     KKADYSIRQLVLVPKHFITPDMIIPRNKGIKNRPNHIMCSINLAPLPESGKIFLIDDS
                     RIIEPETVLKKWQSNLFLRNQNAERKGWLLAVMKCIDQLPEEFTLSQMYEFEEKLSIQ
                     FPQNNHIRDKIRQQLQILRDQNTIEFIGRGLYKKIDKLRPTPKAF"
     gene            complement(7493..10123)
                     /gene="leuS"
                     /locus_tag="ACFM8S_000303"
     CDS             complement(7493..10123)
                     /gene="leuS"
                     /locus_tag="ACFM8S_000303"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFC6313432.1"
                     /translation="MQEQYRPAAIEPAAQKKWDDARIFNVSEDASKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFKLLNGFNVMQPMGWDAFGMPAENAAMKNNVAPAAWTY
                     DNIEYMKTQLKSLGFAIDWARETATCKPEYYRWEQWLFTKLFEKGIVYRKNGTVNWDP
                     VDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAEELLNDLDKLEHWPEQVKT
                     MQRNWIGKSRGMTVRFAVSDDSKQGLEGDYAKFLQVYTTRPDTLMGATYVAVAAEHPL
                     ATAAAAGKPELQAFIAECKAGSVAEADMATMEKKGVPTGRYVVNPLNGDKLEVWIANY
                     VLWGYGDGAVMAVPAHDERDFEFAAKYNLPKKQVIAVGDNAFDANRWQEWYADKENGV
                     LVNSGDLDGLDFQTAFDAVAAKLQSQGAGEPKTQYRLRDWGISRQRYWGCPIPIVHCE
                     QCGDVPVPADQLPVVLPENVVPDGMGSPLAKMPEFYETACPCCGGAAKRETDTMDTFM
                     ESSWYFFRYMSPKFSDGMVSAEAAKYWGAVDQYIGGIEHAILHLLYARFFTKLMRDEG
                     LVNVGEPFERLLTQGMVVCETYYRENDKGGKDWINPADVELTFDDKGRPVSAVLKADG
                     LPVVISGTEKMSKSKNNGVDPQELINAYGADTARLFMMFAAPPEQSLEWSDSGVEGAH
                     RFLRRLWRTVYEYLKQGKAVKAFAGSQDGLSKELKDLRHKLHSTIAKVSDDYGRRQQF
                     NTAIAAVMELLNQYDKTDTGSEQGRTVAQEVLETAVRLLWPIVPHICETLWSELNGAK
                     LWEAGWPTVDEAALVKSEIEVMVQVNGKLRGKITVAADASKADLEAAALATEGAVKFM
                     EGKPAKKIIVVPGRLVNIVV"
     gene            10393..10908
                     /locus_tag="ACFM8S_000304"
     CDS             10393..10908
                     /locus_tag="ACFM8S_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFC6313433.1"
                     /translation="MKIKQIVKPGLAVLAAGVLSACATKSNVKADGTTDNPVFPKPYS
                     VTLDNKRGTFPTYDELDQMRPGLTKDDIYKILGRPHYDEGMYGVREWDYLFHFHTPGV
                     GIDPENTSGVEDVTTCQYKVIFDKDKFARSFYWNPVFPKDAACPPPAPKAEPQVIIRE
                     IVPAKPKRIRQ"
     gene            10915..11358
                     /locus_tag="ACFM8S_000305"
     CDS             10915..11358
                     /locus_tag="ACFM8S_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HFC6313434.1"
                     /translation="MPFRLFLGILCGLSMVAPVYAQGQPDTVGDFIQKKKVIVDTSKA
                     ELCFADDRQCHPVLIGVATPKGTFGLTLNSTDKPGYGGEVIGFKQEGDFLFALHRVWN
                     QIPSERRNERIASPSVSDRIMTNGCINVSDAVYEKLRHYFVLEVI"
     gene            11527..13584
                     /gene="ppk1"
                     /locus_tag="ACFM8S_000306"
     CDS             11527..13584
                     /gene="ppk1"
                     /locus_tag="ACFM8S_000306"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFC6313435.1"
                     /translation="MPEQNRILCRELSLLAFNRRVLAQAEDKNVPLLERLRFLCIVSS
                     NLDEFFEVRMAWLKRENKLHPRRKLDNGKMPSETIADVTEAARSLIRHQYDLFNNVLQ
                     PELAREGIHFYRRRNWTGTQKKWIEDYFDRELLPILTPIGLDPSHPFPRPLNKSLNFA
                     VELDGTDAFGRPSGMAIVQAPRILPRVVPLPSELCGGGHGFVFLSSILHAHVGKLFPG
                     MNVKGCHQFRLTRDSDLTVDEEDLQNLRAAIQNELHDREYGDGVRLEVADTCPAYIHD
                     FLLSQFKLTAAELYQVKGPVNLVRLNAVPDLVNRPDLKFPPHTPGRLKALGKTASIFD
                     LVRQSPILLHHPYQSFDPVVEMMREAAADPDVLAVKMTIYRTGTRSELVRALMKAALA
                     GKQVTVVVELMARFDEANNVNWAKQLEEAGAHVVYGVFGYKVHAKMALVIRREDGVLK
                     RYAHLGTGNYHQGTSRIYTDFGLITADEQITADVNILFMEITGLGKPGRLNKLYQSPF
                     TLHKMVIDRIARETEHAKAGKPARITAKMNSLIEPTVIEALYRASAAGVQIDLIVRGM
                     CTLRPGVKGLSENIRVRSIVGRQLEHARVYYFHNNGTDDTFISSADWMGRNFFRRIET
                     ATPITAPELKKRVIHEGLTMALDDNTHAWLMQPDGGYIRAAPAEGESEADLQNDLWTL
                     LGG"
     gene            13609..13878
                     /locus_tag="ACFM8S_000307"
     CDS             13609..13878
                     /locus_tag="ACFM8S_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313436.1"
                     /translation="MPSETFPFQTAWFYSNLNRADRSQKHTFAIPAQALPLYAPNPKP
                     PHSRRHYPSDSRTKPRIRLHGSGYISNTGIVFRKTVKSASASQYI"
     gene            complement(13847..14950)
                     /gene="dnaN"
                     /locus_tag="ACFM8S_000308"
     CDS             complement(13847..14950)
                     /gene="dnaN"
                     /locus_tag="ACFM8S_000308"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFC6313437.1"
                     /translation="MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGKGGQTK
                     LLATDLEIQIDTAGPEGGAGDFRITTNAKKFQDILRALPAGALVSLDWDDSRLTLKAG
                     KSRFALQTLPAADFPMMNVGEDISATFSLEQERFKTMLSQVQYSMAVQDIRYYLNGLL
                     MQVEGSQLRLVATDGHRLAYAACAIDADLPRAEVILPRKTVLELFKLLNNPDDPIQIE
                     LLDKQVRFQCNGTTIVSKVIDGKFPDFNRVIPLDNDKIFVLSRAELLGALERVSILAN
                     EKFRGARLFLQPGLLSVVCSNNEQEEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHS
                     DDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI"
     gene            complement(15172..16728)
                     /gene="dnaA"
                     /locus_tag="ACFM8S_000309"
     CDS             complement(15172..16728)
                     /gene="dnaA"
                     /locus_tag="ACFM8S_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFC6313438.1"
                     /translation="MTLAEFWPLCLRRLHDMLPQGQFAQWIAPLTVGEEGGVWVVYGK
                     NQFACNMLKSQFAGKIEAVREELAAGRSAFVFKPGEGVRYEMAVVEGAVEPAEPSLHA
                     VSEEMPVQEVLLDELPSEEPVKSVQAKTAADILAERMKNLPHEPRHTAGSASRPESEV
                     LAKARTDAQRDAEEARYEQTNLSPDYTFDTLVEGKGNRLAAAAAQAIAESPGQSYNPF
                     FLYGSTGLGKTHLVQAVGNELLKNRPDAKVRYMHSDDYIRSFMKAVRNNTYDVFKQQY
                     KQYDLLIIDDIQFIKGKDRTMEEFFYLYNHFHNEKKQLILTCDVLPAKIEGMDDRLKS
                     RFSWGLTLELEPPELEMRIAILQKKAEAAGISIEDEAALFIANLIRSNVRELEGAFNR
                     VGASSRFMNRPVIDIDLARTALQDIIAEKHKVITADIIIDAVAKYYRIKISDVLGKKR
                     TRNIARPRQVAMSLTKELTTLSLPSIGDSFGGRDHTTVMHGIRAVAKLREEDPELAQD
                     YEKLLILIQN"
     gene            17016..17150
                     /gene="rpmH"
                     /locus_tag="ACFM8S_000310"
     CDS             17016..17150
                     /gene="rpmH"
                     /locus_tag="ACFM8S_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFC6313439.1"
                     /translation="MKRTYQPSVTKRKRTHGFLVRSKTRGGRAVLAARRAKGRKRLAV
                     "
     gene            17153..17518
                     /gene="rnpA"
                     /locus_tag="ACFM8S_000311"
     CDS             17153..17518
                     /gene="rnpA"
                     /locus_tag="ACFM8S_000311"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFC6313440.1"
                     /translation="MDYRFGRQYRLLKTDDFSSVFAFRNRRSRDLLQVSRSNGNGLDH
                     PRIGLVIGKKTAKRANERNYMKRVIRDWFRLNKNRLPPQDFVVRVHRKFDRSTAKQAR
                     AELAQLMFGNPATGCGKQV"
     gene            17583..17804
                     /gene="yidD"
                     /locus_tag="ACFM8S_000312"
     CDS             17583..17804
                     /gene="yidD"
                     /locus_tag="ACFM8S_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFC6313441.1"
                     /translation="MNFLLSKLLLGLIRFYQYCISPLIPPRCRYTPTCSQYAVEAVKK
                     YGAFKGGRLAIKRIARCHPFGGHGHDPVP"
     gene            17976..19613
                     /gene="yidC"
                     /locus_tag="ACFM8S_000313"
     CDS             17976..19613
                     /gene="yidC"
                     /locus_tag="ACFM8S_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFC6313442.1"
                     /translation="MDFKRLTAFFAIALVIMIGWEKMFPTPKPVPAPQQAAQQQAVTA
                     SAEAALAPATPITVTTDTVQAVIDEKSGDLRRLTLLKYKATGDETKPFVLFGDGKEYT
                     YVAQSELLDAQGNNILKGIGFSAPKKQYSLEGDKVEVRLSAPETRGLKIDKVYTFTKG
                     SYLVNVRFDIANGSGQTANLSADYRIVRDHSEPEGQGYFTHSYVGPVVYTPEGNFQKV
                     SFSDLDDDAKSGKSEAEYIRKTPTGWLGMIEHHFMSTWILQPKGGQSVCAAGDCRIDI
                     KRRNDKLYSTSVSVPLAAIPAGAKAETAVNLYAGPQTTSVIANIADNLQLAKDYGKVH
                     WFASPLFWLLNQLHNIIGNWGWAIIVLTIIVKAVLYPLTNASYRSMAKMRAAAPKLQA
                     IKEKYGDDRMAQQQAMMQLYTDEKINPLGGCLPMLLQIPVFIGLYWALFASVELRQAP
                     WLGWITDLSRADPYYILPIIMAATMFAQTYLNPPPTDPMQAKMMKIMPLVFSVMFFFF
                     PAGLVLYWVVNNLLTIAQQWHINRSIEKQRAQGEVVS"
     gene            complement(19694..20419)
                     /locus_tag="ACFM8S_000314"
     CDS             complement(19694..20419)
                     /locus_tag="ACFM8S_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFC6313443.1"
                     /translation="MSEGMVSPVLYLIPTPLGTPDTPCLLPHEQRAVVGLTDFVVEAE
                     KTARAHLKHLGVTMPIRELNLQTLNEHTDLKTLPELLKPLQEGRSMGIVSEAGCPAVA
                     DPGANLVALAHKHGFEVRPLVGPSSLLLALMASGANGQNFAFKGYLPSEKNERIQSLK
                     ALEQRSRQQNETQLFIETPYRNDVLLADAVENLHSETRLCTATDLTLPTQEIISQTVV
                     QWRKRKEMPNLKKRPTIFVMYAG"
     gene            complement(20466..21056)
                     /locus_tag="ACFM8S_000315"
     CDS             complement(20466..21056)
                     /locus_tag="ACFM8S_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HFC6313444.1"
                     /translation="MGLELPLILGTSSVFRREQMERLGIAFQAASPDFDETPMLGESA
                     PQTALRLAEGKARSLAGRFPEALIVGADQVAWCDGRQWGKPMNLANAQKMLMHLSGRE
                     IEFYSAVVLLNTVTGRMQRHIDKTVVVMRKLDELHILRYLEREPDAVYCSCALKSEAL
                     GALLIERIESTDPNALIGLPVFRLVDFLKNEGVEVL"
     gene            21204..21707
                     /locus_tag="ACFM8S_000316"
     CDS             21204..21707
                     /locus_tag="ACFM8S_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFC6313445.1"
                     /translation="MSDPNLIDPEIFAAEGQNLQGSFLLEELDERVSSHDYPADRQTK
                     ISFTLTGGRDRLQRLFLDLNVKADMPLICQRCIKPMPFMLDESSRIVLFSNEESLDES
                     MLADEELEGILIEKELDVRTLVEDQILMSLPFSPRHEDCGDNGTLEEVNRDKPNPFAV
                     LAGLKSS"
     gene            21741..21920
                     /gene="rpmF"
                     /locus_tag="ACFM8S_000317"
     CDS             21741..21920
                     /gene="rpmF"
                     /locus_tag="ACFM8S_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFC6313446.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDALTAPALSVDSTTGEVHRPHHISP
                     NGMYRGRKVVKAKGE"
     gene            22095..22622
                     /locus_tag="ACFM8S_000318"
     CDS             22095..22622
                     /locus_tag="ACFM8S_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HFC6313447.1"
                     /translation="MKQKIWYTYDDIHRVIKALAEKIRNADIKYDAMIAIGGGGFIPA
                     RMLRCFLEIPIYAVTTAYYDSDNEGQVTEEVKKVQWLDPVPEALRGKNVLVVDEVDDS
                     RVTMEFCLTELLKEDFGTIGVAVLHEKIKAKAGKIPEGIPYFSGITVEDWWINYPWDA
                     LDIDEHNRLAEAGRG"
     gene            22712..23767
                     /gene="plsX"
                     /locus_tag="ACFM8S_000319"
     CDS             22712..23767
                     /gene="plsX"
                     /locus_tag="ACFM8S_000319"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFC6313448.1"
                     /translation="MITLAVDAMGGDQGLAVTVPGATAFLQAHPDVRLIMTGDETQLR
                     QALTAAGAPMERIDICHTAQIVGMDEAPQSALKNKKDSSMRVAVNQVKEGKAQAAVSA
                     GNTGALMATARFVLKTIPGIERPAIAKFLPSDSEHVALALDLGANVDCTPEQLVQFAI
                     IGSELVHALHPEKGSPRVALLNVGTEDIKGTDAVKQTFKLLSSSKLNFIGNIESNSVL
                     YGEADVVVADGFVGNVMLKTIEGAVKFMSGAIRREFQSNLFNKLAAVAALPALKGLKN
                     KLDPRKFNGAILLGLRGIVIKSHGGTDETGFRYALEEAYHEAKSASLSKIEQGVAEQL
                     AALEAAKAAQNEIAADL"
     gene            23905..24156
                     /locus_tag="ACFM8S_000320"
     CDS             23905..24156
                     /locus_tag="ACFM8S_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HFC6313449.1"
                     /translation="MKTLEKRMKALDKRMMKFGKSLEGRLDARLIESALDYIHYSERF
                     LAFEILCTYIEDFDVRLTEQESREISFINKEFEIESTSD"
     gene            24183..24599
                     /locus_tag="ACFM8S_000321"
     CDS             24183..24599
                     /locus_tag="ACFM8S_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313450.1"
                     /translation="MKTSTVVFGGFFMADNGERIQIPVLENPDIREINHFFSISNFEK
                     KAGVLVFRIIPEPEFGDTELTVYFEKGYYLPMIQTILEGGDIEVKNLKTENYSGNTME
                     IWGDSYPIEHISKNISTIQNIISEFIIQKQTPAPII"
     gene            24619..24822
                     /locus_tag="ACFM8S_000322"
     CDS             24619..24822
                     /locus_tag="ACFM8S_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313451.1"
                     /translation="MPSEACLVFRRHFLRLKQRIGNPAPPVHGNLHLKTICIPNHTPI
                     PVSVICTLPVLGNHRFFAYNSRI"
     gene            24849..25811
                     /locus_tag="ACFM8S_000323"
     CDS             24849..25811
                     /locus_tag="ACFM8S_000323"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFC6313452.1"
                     /translation="MQYAKISGTGSYLPANRVSNDDLAQKVDTSDEWITARTGIKFRH
                     IAAENEKTSDLAAEAARRALDAAGLDSGEIDLIIVATATPDMQFPSTATIVQQKLGIT
                     NGCPAFDVQAVCAGFMYALTTANAYIKSGMAKNALVIGAETFSRIVDWNDRTTCVLFG
                     DGAGAVVLSASDKPGIIHSKLKADGNYLKLLNVPGQIACGKVSGSPYISMDGPGVFKF
                     AVKMLSKIADDVIEEAGYTAAQIDWIVPHQANRRIIESTAKHLGLSMDKVVLTVQDHG
                     NTSAASIPLALDTGIRSGQIKRGQNLLLEGIGGGFAWGAVLLQY"
     gene            25858..>26174
                     /locus_tag="ACFM8S_000324"
     CDS             25858..>26174
                     /locus_tag="ACFM8S_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFC6313453.1"
                     /translation="MKRQAFFKLMACAAFLSAVSLRLPVLGACYAILSLYAFALYGID
                     KRRAVRGKRRIPEHRLLLPALFGGWAGAYLGSRIFRHKTAKKRFVVLFRLTVSGNVLA
                     TCIL"
BASE COUNT         6575 a         7506 c         6329 g         5764 t
ORIGIN      
        1 gcctgcacat tgaaaatgcc gcccaagcaa accttcagtt tgccagcctc gtcctagccc
       61 tctcccacgg gagaggggat tggggtgcag gctgccttta aggttcaggc aaattttaac
      121 ttcgttgata ctgcgcttta gcttcgcaga agctgcactt tcagacgacc ttttggaaca
      181 ccacaggtac acgcatttaa ggaatgccgt ctgaaatgcc tgcctcaata acgcatcatg
      241 ttgccgtcaa tctcggccgc ccatgcatcg atgccgccct gaaggttgta caggttttca
      301 aaacccgcgt ccgccaaata catcgccgta tgcaggctgc ggataccgtg atggcaatac
      361 accacaagcg gcacatcatc cggcagctcg ttctgccgca gcggaatcag attcatcggg
      421 atatgcagcg catttggcag cgaacaaacc gccgtttctt catccgtacg cacgtccaac
      481 aaacaaaaca tccgcccttc gtccatccac gctttcaatt ccgccgaccc aagttgcaca
      541 atatccatcg caccccccaa aaaaaaccaa gcaaaatgcc gtctgaagcc ccaaacccgc
      601 tttcagacgg catcgcaatc cgaacatcaa aactgaaaac cgccaaacgg atcggcatcc
      661 ttatcatcca aatgcgccac caaggtatcg aacagcacct tctcttcaaa cacatcgccc
      721 ctgcgcgtaa tcaaaagcgc gcgttgcacc ggcctgcggc ctacgataac caccatgcgt
      781 ccgccatctt tcaactgttc tttcaacact tcaggcacaa ggtttaccgc accgccgaca
      841 taaaccgcat caaacggcgc acctgcggaa agttcggtca acccgttgtt ttgcacataa
      901 tcaatgtttt ccaaagacaa gccgtccaac accgctttgg cgcggttttg ctgttcggca
      961 tcgatatcgt ccgacaccac acgactagcc aattttgcca aaagcgcggt cgcatagccc
     1021 gaacccgtgc cgatttccaa aaccgtatcg tttttcgtca gcttcaagcc ctgcgccagc
     1081 cgcgccacga ctttcggctc cagcatctta tgaccgttgg caagcggcag cgccatatcc
     1141 gcatacgcca aaccctgcaa atcctcatcg acaaaaagct cgcgcggaat ctccgccaaa
     1201 gcgtccaaaa cgtcaaaatc caatacatcc caaggacgga tttgctgttc gaccatattg
     1261 aaccgcgctt tttcaaaatc catttagtgc tccgtcaaat aattgttcca acaatagcag
     1321 gcattataaa accacactcc cgccgcgcca acttcggcac gcgccgacag ctcgttttgc
     1381 cagtctcaaa ccgcctgacg cgaagcctca aaccgcttct ccaaaatctt cgccagttcg
     1441 cccaaataca aagcatctgt ctcatcaaac tgcgccaaat gttcgctgtc cgcgtccaac
     1501 acgccgatac agcggccgtc tgaaaacagc ggcacgacaa tctccgaacg cgacaaagac
     1561 gaacaggcaa tatggtcggg atgcgcgtcc acatccccga cgaccaccgt cccgcccttc
     1621 gcccaagcct gaccgcacac cccgcgtccg aacggaatcc gcgtacacgc caaaggtccc
     1681 tgaaacggtg ccaaaaccaa ttcgtccgaa cgcgtatcga ccaaataaaa acccacccaa
     1741 aaccagccga acgcctcctt caaaaccgcc gccgtgttcg ccaaattcgc cacccaatcc
     1801 gtctcgtccg ccaccacaga ctcaatctgc ggcaacacct cccgataaag cgcggccttg
     1861 tccgaagccg aaaaatgaag cgcgtgcatc acatctccta tagttgcata catatcaggc
     1921 ggccattata aaacaacctg cccgaaacaa cattccaaac cgcccccgca gccgcccgcc
     1981 ccaagttgcg aacccgccgc atatccacta aacttcacat tgcaacgcgc cacacgcggc
     2041 agacagaaaa aacacgacac ggagcaaaaa agatgtatca ccaaatcgga atgtgggatc
     2101 aaaaatgggt catcggcaac tggaaaatga acggccggct ccagaacaac aattcgctca
     2161 tgcaccgctt ccgcatcctc cccaccgccg aacgcgtcct catcggactc gccgccccga
     2221 ccgtttacct gctgcaactg cacaacgcca tgcaaatcgt cttaaacaac cgcatcctca
     2281 cctgcgccca agacgtgagc cgcttccccg acaacggcgc gtacaccggc gaagtgtccg
     2341 ccgaaatgct cgccgacacc ggcacagaca tcgtcctcat cggacactcc gaacgcagcc
     2401 tttatttcgg cgaaaaaaac gaaatccaac gccgcaaaat ggaaaacgtc ctcaacgtcg
     2461 gactcatccc gttattgtgc gtcggcgaaa gcctcgaaga gcgcgaagcc ggcaaagaac
     2521 acgaagtcat cgcccatcag ctttccatcc tgcaagggct ggataccaaa aacatcgccg
     2581 tcgcctacga acccgtctgg gcgatcggca ccggcaaagt cgccaccgtc gaacagattg
     2641 ccgatatgca cgcattcatc tacaaagaaa tcttgtcttt gtgcggaagc gatgttaaaa
     2701 tccgcgtcct ttacggcgga agtgtgaaag cggacaacgc ggccgacatc ttcgcagtac
     2761 cttatgtgga cggcgcactc gtcggcggcg cgtcattgtc gtacgactcc tttaccgcca
     2821 tcatcagtgc cgcacaaaat gcgtagaaaa atatggaagc cttcaaaacc ctaatttgga
     2881 ttgttaatat aatttccgct ttggccgtca tcgtgttagt attgctccaa cacggcaaag
     2941 gcgcggatgc cggagcgact ttcggatcgg gaagcggcag cgcgcaaggc gtattcggct
     3001 ctgccggcaa cgctaacttc ctcagccgct cgaccgccgt tgcagcaaca tttttctttg
     3061 caacctgcat ggctatggtg tatattcaca cccacacgac aaaacacggt ttggacttca
     3121 gcaacgtaca acaaactcag caagcaccca aacccgtaag caataccgaa ccttctgccc
     3181 ctgttcctca gcagcagaaa taacagtttt tcaaatgccg acatggtgaa attggtagac
     3241 acgctatctt gagggggtag tggccgtagg ctgtgcgagt tcaaatctcg ctgtcggcac
     3301 caacacacaa aaacgcctga aaatttcagg cgttttttgt tcaatcccat ccaacgctgt
     3361 caaccaattc caatcaatac aaggatttca ggctgattgt tatcctgccg tcccccttcc
     3421 tgacagtgca atcccgtcca atccgcccta attgaagtaa cctaaaattt acggtatctt
     3481 ttgcggtatc tgaaaaatac ctcgaaaaaa taccgcaaaa ataaagctga acgaccgcca
     3541 aatcaggaat gccaagcgga aaagagcttg cggggaatac tgccaagacg tagggaacaa
     3601 gggagaaacc gtccaagatg cagggcggtt ttttttgggt ttttggaaaa aacctatact
     3661 aggaagcgat acccttagtt gttaccttgt taccggggaa aagttagata aataagcata
     3721 tgaaatatag tgaattaaat ttaaatcagg acaaggcggc gagccgcaga cagtacaaat
     3781 agtacggcaa ggcgagccaa cgccgtaccg gtttaaattt aattcactat aaaataagaa
     3841 aaagataaaa aattggtaac aaatgcggta acaaatggta acgaatcggt aacaactttt
     3901 ggggttttcc ggtttttcac cgtcttggca gtgggagcgt agcggaatga aaagccaaaa
     3961 cgcacggaac cgcgcctatt ttgagcagga atggcggtta aaccgcttgg ttatatacgg
     4021 ggggaatagg aagacagcga aacgcgctaa aaacgcaatt tgagccatta aaagcgattg
     4081 attaaaaaat aatcaggtta gccgccagtg tttcaggcgg cataaacgga gaaattgtgg
     4141 ggcataaaaa aggcagcttg ccgtgttgtc tgtctctggt ataattccaa gtatcactaa
     4201 tcaacggcta cacaatgcgg atattcaaaa accaatggat agtgaaattt gccaagaaac
     4261 ataaaatcag tgattccgag ctgctggaag cggtagagag ggcggataac gggctgatag
     4321 acgcagattt aggcggcggt gtgattaagc agcgcatagc aaggcaagga caaggcagaa
     4381 gcggcggcta tcgcagcctg atattgttca aacaggcaga tagggcattt ttcgtttacg
     4441 cctttgccaa gaacgacaga gaaaacattt cggataaaga actggacgtt taccgaaaag
     4501 ccgccgcata ttatctgaaa tacacgcggg cagagctggc ggctttgaaa gaagacggca
     4561 ttatcacgga gatagaatca tgaaatacaa aagcgaggca ttagccgcca ttcatgaaat
     4621 gatggaaggg gcttacaaca tcggcgcaat cgacaaaaag actatgcgcg gctttgacaa
     4681 gtcctgcctg accgaaatca aaccgttgag cggcggagac atcaaggcaa tcagggagaa
     4741 ggaggcacta tcgcaagccg ctttcgccat ctatctcaac gtgggaaaaa atcacgtttc
     4801 ggcttgggag cggggcgtta aaaagcccag cggcgcggcg ttgaagctgc tgaccatcgt
     4861 caaaaacaag ggcatcgaag ccattgcgta gacgacttgg caaacggcaa aatcagcaag
     4921 ttcacaatag acgcgctgct gaatatgcct gacgaaacag gcaagaccgc cgaaccgaat
     4981 atcagggcgt agccgcacaa atgcccgacc gcatcaaacc aagccgaaac ggcggcggtg
     5041 cagacggcat agcccgacag caaggcacgg cgcagacggg cgcgaaaccc gaaacatccc
     5101 cgaccgcgag gcacggggat ttttttgcgc ccgttgcagg ggggattgga tttaagcggc
     5161 gcgggcttga aggcaaaacg ggcggggcgc agaactgttt aaatgccgtc tgaatctcaa
     5221 acgatttcag acggcatttt gaaacaacgg ctcaaattct cgatcccctt cccttaacgc
     5281 cgacgttttt tattaacgcg ccccttattt ctgacacttt gctcataaac cggcataacg
     5341 gtcggcaaca accgttttag attttctata cgggcattgt ttgtcggatg agtagaggta
     5401 atagcataaa taaagccgtt ttggtcgttt tcctgattca ttttttccca aaccctgaca
     5461 gcggccgccg gatgatagcc tgcctgcgcc atcaacatca ttcccccctc atcggcttct
     5521 tcttccaagc tgcgactata aggaaaggta atgccgtacg ttcccaaaat atccataccc
     5581 aatccgacca attccggatt agtgtccggt tttttgtcta atataatctg cgtgcctatc
     5641 tgcgccgccg tattggtcaa aatttgttgc ccgaccttat ttttaccgtg ttcatgcagg
     5701 gcgtgcgtca tttcatgccc cataatggcg gcaatttcgt catcggtcag cttgagtttg
     5761 tcgactatcc ccgtataaaa cgccattttt ccaccgggca ttgcccacgc gttcagctca
     5821 tcgtttttga aaaccgtcat tttccagtca aacttatggc cggtattatt tgccgcatcg
     5881 gcataaggca gcatacgtcg aaatactgcc tgcaccctgc gggctgttct ggatgtggta
     5941 tcgacattgc cggcagactt gttcaactca accgttttca tataatcttt ggcagccgca
     6001 gcgttcattg tggcggaatc atgaccgtaa acatcagcaa cgaccgcaca acctcccaat
     6061 atcgagattg ctgccgacag acagactatc cgtttaaagg aaggaaggaa ggaaggaagg
     6121 aaatttcata ttaggcttat cccttaaaaa attaaattca aaaaatgccg tctgaatcca
     6181 aaacggattt cggacggcat cttaacattg tttaatgttt ttaaaaagat ttacaccacg
     6241 atgttcacca gtctgcccgg tacggcgatg attttcttgg caggattacc ttctatgaat
     6301 ttcactgcgc cttcggaggc ggccggtttg tcggaatcac gcataaattg ccaataattc
     6361 cccgaccttc ttacggctgc tgccttttgc ggcaatattg cgctgtactt caactgtttt
     6421 cagaatagca gaagaataaa tatcccttgt gaattcagta tcatgatttg aaattaaaac
     6481 gccttgggag tcggacgcaa tttgtcgatt tttttgtaaa gtccgcgacc aatgaattcg
     6541 atcgtatttt ggtcgcgcaa aatttgcaac tgttggcgga ttttgtctct gatatggttg
     6601 ttttggggaa attggatgga tagtttttct tcaaattcat acatttgcga caatgtgaat
     6661 tcttcgggaa gttggtcgat acatttcata acagccaaaa gccaaccttt gcgctccgca
     6721 ttttggttgc gtaaaaacag attggattgc cattttttca gaacggtttc gggttcgata
     6781 atgcgggaat cgtctattaa gaatattttt ccgctttcag gcaaaggggc gagattgatg
     6841 gaacacataa tgtggttcgg ccggttttta atgcctttat ttctgggaat aatcatatcc
     6901 ggcgtgatga aatgtttggg tacaagcacc aattgccgta tggagtaatc cgctttttta
     6961 tatgcaagaa agaaaaagtt tgggttggta tctgaccgga tgcgctccaa catggtgcga
     7021 tatgcaccgt caggcacact gttgcctatg gttttttgat ttttactctt taattcatat
     7081 tgctcgtggc aatttgggca aaagaggtct gcaacaggtt tgttattggc aaatctctgc
     7141 atcggcttgc ttccgcaaca gggacagtag ccgttttttt ccagccaagc ctcgctcatt
     7201 acacggattt tatgggttgc tttattttgt tgctttccca attcggtatc gaaaaataaa
     7261 ttcatgtttt ggattttgag attaagtttt agtggattaa caaaaatcag gacaaggaga
     7321 cgaagccgca gacagcaggg caaggcgggg caacgccgta ccggtttaaa gttaatccac
     7381 tatatttgag gtctgtcatg cggacaaccc aatctgcctt aaaaaggccg tctgaaaccc
     7441 tgtttccaag tttcagacgg cctttatccg tgtggctaaa ccttaaaagc ggttagacga
     7501 cgatgttcac cagtctgccc ggtacgacga tgattttctt cgccggtttg ccttccatga
     7561 atttcaccgc gccttcggtg gcgagtgcgg cggcttcgag gtcggctttg gatgcgtcgg
     7621 cggcaacggt gattttgccg cgcagtttgc cgttgacttg aaccatgact tcgatttcgg
     7681 atttgaccaa ggcggcttcg tcgactgtcg gccagcctgc ttcccacagt ttcgcgccgt
     7741 tcaattcgct ccacagggtt tcgcagatgt gcggcacgat gggccacagc aggcgtacgg
     7801 cggtttccaa tacttcttgg gcgacggtgc ggccttgttc gctgccggtg tcggttttgt
     7861 cgtattggtt gagcagttcc atcacggcgg cgatggcggt gttgaactgt tggcggcggc
     7921 cgtagtcgtc gctgactttg gcgatggtgg aatgcagttt gtggcgtagg tctttgagtt
     7981 ctttagacaa accgtcttgg ctgcctgcga acgctttgac cgctttgcct tgtttcaggt
     8041 attcgtaaac ggtgcgccac aggcggcgca ggaagcggtg tgcgccttcg acgccgctgt
     8101 cgctccattc gagggactgt tcgggcggtg cggcgaacat catgaacagg cgggcggtgt
     8161 ctgcgccgta ggcgttaatc agttcttgcg gatcgacgcc gttgtttttg gacttggaca
     8221 ttttttccgt gccgctgatg acgacgggca gtccgtcggc tttgaggacg gcggaaacgg
     8281 ggcggccttt gtcgtcaaaa gtcagctcga catcggcagg gttgatccag tctttgccgc
     8341 ctttgtcgtt ttcgcggtag taggtttcgc aaacgaccat accttgcgtc agcaggcgtt
     8401 cgaacggttc gccaacattg accaaacctt cgtcgcgcat cagtttggtg aagaaacgcg
     8461 cgtacaggag gtgcaaaatg gcgtgttcga taccgccgat gtattggtcg accgcgcccc
     8521 agtatttcgc ggcttccgcc gataccatgc cgtctgaaaa cttgggcgac atatagcgga
     8581 agaaatacca gctcgattcc atgaaggtgt ccatggtgtc ggtttcgcgt tttgccgcgc
     8641 cgccgcagca tgggcaggca gtttcgtaaa actcgggcat ttttgccagc ggcgaaccca
     8701 tgccgtcggg tacgacgttt tcaggcaaaa cgaccggcaa ttggtcggca gggacgggta
     8761 cgtcgccgca ttgttcgcaa tggacgatgg gaatcgggca gccccagtag cgttggcgcg
     8821 aaatgcccca gtcgcgcagg cggtattggg ttttcggttc gcccgcgcct tggctttgca
     8881 gcttggcggc aacggcatcg aatgccgtct gaaaatccaa gccgtccaag tcgccgctgt
     8941 tgaccaatac gccgttttct ttgtcggcat accattcttg ccatcggttt gcgtcgaatg
     9001 cgttgtcgcc gacggcaatg acttgttttt taggcagatt gtatttggcg gcgaactcaa
     9061 aatcgcgttc gtcgtgcgcc ggaaccgcca tcactgcgcc gtcgccgtag ccccacaata
     9121 catagttggc aatccacact tccagcttgt cgccgttgag cggattgacg acgtagcggc
     9181 cggtcggcac gccttttttc tccatcgtcg ccatatcggc ttcggcaacg gagccggctt
     9241 tgcattcggc gataaatgcc tgcaattcgg gtttgccggc ggctgcggcg gttgccagcg
     9301 gatgctcggc ggcaacggcg acataagtcg cgcccatcag cgtgtcgggg cgggtggtat
     9361 aaacttgcag gaatttcgcg taatcgcctt ccaaaccttg tttgctgtcg tctgaaacgg
     9421 cgaagcgcac ggtcataccg cgcgatttgc cgatccagtt gcgctgcatg gttttgactt
     9481 gttccggcca gtgttccagc ttgtccaagt cgttgagcag ctcttcggcg taatccgtga
     9541 ttttgaagta atacatcggg atttcgcgtt tttcgatcaa tgcgccggaa cgccagccgc
     9601 gtccgtcgat gacttgctcg ttggcaagga cggtttggtc gacagggtcc cagttcaccg
     9661 tgccgttttt gcgatacacg atgccttttt caaacagctt ggtaaacagc cattgttccc
     9721 agcggtagta ttcgggtttg caggttgcgg tttcgcgcgc ccagtcaatc gcaaaaccta
     9781 ggcttttgag ctgggttttc atgtattcga tgttatcgta cgtccaagcg gcaggggcga
     9841 cgttgttttt catcgccgcg ttttccgccg gcatgccgaa cgcgtcccaa cccataggct
     9901 gcatgacgtt gaagccgttt aaaagtttga agcggctcaa tacatcgccg atggtgtagt
     9961 tgcgcacatg ccccatgtgc agcttgccgc tgggataggg gaacatggag aggcaataat
    10021 atttgggttt ggaagcgtct tcggagacgt tgaaaatacg ggcgtcgtcc cattttttct
    10081 gcgccgcagg ctcaatggcg gcgggccggt attgttcttg catagtcatt ctgttttcgc
    10141 ttaaaaacgt tggaaaaata aagtcggcat caattataac aggttgccgg aagcggcgaa
    10201 tcggcagatt gccggcagga tgcgtaaatt cgcacgcgca ttattccgta tgccgtacaa
    10261 atacaccgcg tttattgata cgcacgtttt ttatgctaat attacaaacc aaaatcaaat
    10321 gtttaaaact ctcctgatgc ggctcttccg aacaaaaggc agacgggcat cgggtaaaag
    10381 aggattctgc atatgaaaat caaacaaatc gtcaaaccgg gcttggcagt attggcggcg
    10441 ggcgttctgt ctgcctgcgc aaccaaaagc aacgtcaaag ccgacggcac gaccgacaat
    10501 ccggttttcc cgaaacccta ttccgtaacg ctcgacaaca agcgcggcac attcccgact
    10561 tatgacgaac tggatcagat gcgccccggc ctgaccaaag acgacatcta caaaatcctg
    10621 ggccgcccgc attacgacga aggtatgtac ggcgtgcgcg aatgggatta cctgttccac
    10681 ttccataccc cgggcgtagg tatcgaccct gaaaacactt ccggcgtaga agatgttact
    10741 acctgccaat acaaagtgat tttcgataaa gacaaatttg cccgcagctt ctactggaac
    10801 cccgtcttcc cgaaagatgc cgcctgtccg ccgcccgcac ccaaagccga gccgcaagtc
    10861 atcatccgcg aaatcgtgcc ggcaaaaccg aaacgtatcc gccaataatc cgacatgccg
    10921 ttccgcctgt ttttagggat attatgcggc ctgtcaatgg ttgcccccgt atatgcacag
    10981 gggcagccgg atacggtcgg cgactttatc caaaagaaaa aagtcatcgt cgatacatcc
    11041 aaagcggaac tctgtttcgc tgacgaccgt cagtgccacc ccgtcctcat cggtgttgcc
    11101 acgcccaagg ggacgttcgg gctgacgctg aacagtaccg acaagcccgg atacggcggc
    11161 gaagtcatcg gtttcaagca ggagggtgat tttcttttcg ccctgcaccg cgtttggaat
    11221 cagataccgt cggaaaggcg gaacgaacgc atcgcctccc cgtccgtgtc cgacaggatt
    11281 atgaccaacg gctgcatcaa cgtcagcgat gcggtgtacg aaaaactgcg tcattatttt
    11341 gtgttggaag tgatttgaaa cagacggata ccgcacgcgc cggtatccgt ttccacattg
    11401 ccccgatgcc tgaaacagac cgtccgccac atcatgccgt ctgaaaccgg cgcagatgcc
    11461 gccaagcctt cagacggcat tgtccgcccg ctccggccga acaacaacca tctttgggag
    11521 agccttatgc ccgaacaaaa ccgcatcctc tgccgcgaac tgagcctgct ggcattcaac
    11581 cgccgcgtgt tggcgcaggc ggaagacaaa aacgttcccc ttttggaacg cctgcgcttc
    11641 ctgtgcatcg tgtcgtccaa tcttgacgag ttttttgaag tccgcatggc gtggctcaag
    11701 cgtgaaaaca agctgcaccc ccggcgcaag ctggacaacg gcaaaatgcc gtctgaaacc
    11761 atcgccgacg ttaccgaagc ggcgcgctcc ctgatacggc accagtacga cctgttcaac
    11821 aacgtccttc agcccgagct ggcgcgggaa ggcatccatt tttaccgccg ccgcaactgg
    11881 acgggcacgc agaaaaagtg gatcgaagac tatttcgacc gcgaactgct gccgattctg
    11941 acccccatcg gccttgaccc ctcccacccc ttcccgcgcc cgctgaacaa atcgctcaac
    12001 ttcgccgtcg aactcgacgg cacagacgcg ttcggcaggc cttcggggat ggcgattgtg
    12061 caggcaccac gcatcctgcc gcgcgttgtt cccctgccgt ccgaactgtg tggcggcgga
    12121 cacggcttcg tcttcctctc ctccatcctc cacgcccacg tcggcaaact cttcccgggc
    12181 atgaacgtca aaggctgcca ccagttccgc ctgacgcgcg acagcgactt gaccgttgac
    12241 gaagaagacc tgcaaaacct ccgcgccgcc attcaaaacg agttgcacga ccgcgaatac
    12301 ggcgacggcg tgcggctcga agtcgccgac acctgccccg cctacatcca cgacttcctg
    12361 ctgtcgcaat tcaaactgac cgccgccgaa ctctatcagg tcaaaggccc ggtcaacctc
    12421 gtgcgcctca acgccgtccc cgacctagtc aaccgccccg atttgaaatt tcccccacac
    12481 acgccgggca gactgaaagc cttgggcaaa accgcgtcca tattcgattt ggtgcgccaa
    12541 tcgcccatcc tgctgcacca cccctaccaa tcgttcgacc ccgttgtcga aatgatgcgc
    12601 gaagccgccg ccgaccccga cgtgcttgcc gtcaaaatga cgatttaccg caccggcacg
    12661 cgctccgaac tcgtccgcgc cctgatgaag gcggcactcg ccggcaaaca agtaaccgtc
    12721 gtcgtcgaac tgatggcgcg ttttgacgaa gccaacaacg tcaactgggc gaagcagctc
    12781 gaagaggcgg gcgcgcacgt cgtgtacggc gtgttcggct acaaagtcca cgccaagatg
    12841 gcactggtca tccgccgcga agacggcgta ctcaagcgtt acgcccatct cggcacgggc
    12901 aactaccacc aaggcacatc gcgcatctac accgacttcg gcctcattac cgccgacgaa
    12961 caaatcaccg ccgatgtgaa catattgttt atggaaatca caggtttggg caaacccggg
    13021 cggctgaaca aactctacca aagcccgttt accctgcaca aaatggttat cgaccgcatc
    13081 gcacgcgaaa ccgaacacgc aaaagccggc aaaccggcgc ggattaccgc caagatgaat
    13141 tcgctcatcg aaccgaccgt catcgaagcc ctgtatcggg caagcgcggc aggcgtacaa
    13201 atcgatttga ttgtgcgcgg tatgtgcacc ttgcgcccgg gtgtaaaagg cttgtccgaa
    13261 aacatccgcg tccgctccat cgtcggcagg cagctcgaac acgcgcgcgt gtattacttc
    13321 cataacaacg gcacggacga tacctttatc tccagcgcgg attggatggg gcgcaacttc
    13381 ttccgccgca tcgaaaccgc cacgccgatt accgcgcccg aactcaaaaa gcgcgttata
    13441 catgaaggac tgaccatggc actggacgac aacacccacg cgtggctgat gcagcccgac
    13501 ggcggctata tccgcgccgc acctgccgag ggcgaatccg aagccgacct gcaaaacgat
    13561 ttgtggacac tgctcggagg ctgacccgca ccgccccaat caaaaaccat gccgtctgaa
    13621 acctttccgt ttcagacggc atggttttac agcaatctaa acagggcgga ccggagtcaa
    13681 aaacacacct tcgccattcc tgcacaagca cttcccctat acgctcccaa ccccaagccg
    13741 ccgcattcca gacggcatta cccttccgac agccgaacaa aaccccgaat ccgtctgcac
    13801 ggttcggggt atatctccaa tacgggcatc gtgttccgga aaaccgtcaa atccgcatcg
    13861 gcatcacaat atatttgaaa ttcggattgt tcggcacggt aaacagcgtc gagcggttgg
    13921 catcgccgaa ggcaagctgc atatcgtcgg aatggatgtt gcgcaacacg tccatcagat
    13981 agccgatatt gaaaccgact tcgagttcgc cgccctgata ggcgatttcg atttcttcgc
    14041 gcgcttcttc ctgctcgttg ttgctgcaca caacgctcaa caggccgggt tgcaggaaca
    14101 ggcgcgcgcc acggaatttt tcgttggcaa gaatggacac gcgttccagc gcacccaaaa
    14161 gttcggcacg ggacaaaacg aaaatcttgt cgttgtccaa aggaatcacg cggttgaaat
    14221 cggggaattt gccgtcgatg accttgctga cgatggtcgt gccgttgcat tggaaacgca
    14281 cctgtttgtc cagcagctcg atttgaatcg gatcgtcggg gttgttcaac agtttgaaca
    14341 gttccagcac cgttttgcgc ggcaaaatca cttcggcgcg cggcaaatcc gcatcaatcg
    14401 cgcaggctgc ataggcaagg cggtgtccgt cggtcgccac aaggcgcaac tggctgccct
    14461 caacctgcat cagcagaccg ttgagataat agcggatgtc ctgcaccgcc atgctgtact
    14521 gcacttgcga cagcatggtt ttgaaacgct cctgctccag cgagaaagtc gcgctgatgt
    14581 cctcgccgac attcatcatc ggaaaatcgg cggcaggcag ggtctgcagg gcaaaacgcg
    14641 atttgcccgc cttcagcgtc agacggctgt cgtcccaatc cagcgacacc agcgcaccgg
    14701 caggcagcgc gcgcaaaatg tcctgaaatt tcttggcatt ggtagtgatg cggaagtcgc
    14761 ccgcgccgcc ctcgggaccc gcagtgtcga tttggatttc caaatcggtt gccaagagtt
    14821 tggtctgacc gccttttccc tcaatcagga cgttggacag gatgggcagg gtgtggcggc
    14881 gttcgacgat gccggtaacg gcttgcagcg gcttgagcag gctgtcgcgt tcggcttgta
    14941 aaatcaacat gttcgctcct ttaaatcggt ttgtatagtg gattaaattt aaatcaggac
    15001 aaggcgacga agccgcagac agtacaaata gtacggaacc gattcacttg ttaaagaatc
    15061 gttctctttg agctaaggcg aggcaacgcc gtactggttt aaagttaatc cgctatatct
    15121 ttacccttcg gacggcatgg gcaatatcat gtcgtctgaa aacgttttcc atcagttttg
    15181 aatcagaatc agcagctttt cataatcctg agccaattcc ggatcttctt cgcgcagttt
    15241 cgccactgcc ctgatgccgt gcataacggt cgtatggtcg cgcccaccaa acgaatcgcc
    15301 gatagacggc aggctcaaag tggtcagttc tttggtcagg ctcatggcaa cctggcgcgg
    15361 acgggcaatg tttcgtgtcc gtttcttacc gagcacatcg ctgattttga tgcggtaata
    15421 tttcgccacc gcatcgatga tgatgtcggc ggtgatgact ttgtgcttct cggcaataat
    15481 gtcctgcaaa gcggtacgcg ccaaatcgat gtcgatgacg ggacggttca taaagcggct
    15541 gctcgctccg acacggttaa acgcgccttc aagctcgcgc acgttggaac ggatcagatt
    15601 ggcaatgaac agcgcggctt cgtcttcaat actgatgccc gccgcttccg cctttttctg
    15661 caaaatggcg atacgcattt ccaattcggg cggctcgagt tccaaagtca gtccccatga
    15721 aaaacgggat ttgaggcggt cgtccatacc ttcgattttg gcgggtaaaa catcgcaagt
    15781 gaggatgagc tgttttttct cgttgtggaa atggttgtac agatagaaga attcctccat
    15841 cgtacggtct tttcctttaa taaactgaat atcgtcgata atcagcaggt cgtattgctt
    15901 gtattgctgc ttgaacacgt cgtaggtatt gttgcgaacc gccttcataa aactgcggat
    15961 gtagtcgtcc gaatgcatat agcgcacttt ggcatcggga cggtttttca acagttcgtt
    16021 gccgaccgcc tgcacaaggt gggttttacc caaacccgta ctgccataga ggaagaacgg
    16081 gttataactc tgtcccggac tttccgcaat cgcctgcgcg gcagctgccg caaggcggtt
    16141 gcccttgcct tctaccaacg tatcaaatgt gtaatccgga gacaggttgg tttgttcgta
    16201 acgcgcctct tccgcatcac gctgcgcatc cgtccgcgct ttcgcaagta cctccgattc
    16261 cggacgggaa gcagacccgg cagtatggcg cggctcatgc gggaggtttt tcatacgttc
    16321 cgccaaaata tccgccgccg ttttcgcctg tacggatttg acaggctctt cagacggcag
    16381 ctcgtccaac agaacctcct gcacgggcat ttcctccgac accgcatgca aggacggctc
    16441 ggcaggttcg acagcacctt caaccaccgc catctcataa cgcacgcctt ctcccggttt
    16501 gaatacgaag gcggaacggc cggcagccaa ctcttccctc accgcttcta tttttccggc
    16561 aaactggctc ttgagcatat tgcaggcaaa ctggttcttg ccgtacacca cccatacgcc
    16621 accctcctca ccgacggtaa ggggcgcaat ccattgcgca aactgccctt gaggcaacat
    16681 atcgtgaaga cggcggaggc acagcggcca aaactctgct agtgtcatag ataagctcga
    16741 atcggtaaaa atgaaatcga aaacaaagaa aatataatat tttcaaaaag aaaacaaatc
    16801 tgttgaacgc acatcggttc aaaacgcgac tgcccgatta taccgactca cgaatatttt
    16861 atccacaacc cgtgcaaaaa tttatccaca gaaaggcggc agaaatccgc aggcaatcgg
    16921 gcaatcctcc tgcaaagttt ctatattgat tgacaaaagc ggcaaattgg agtgtaattc
    16981 acggtttatc tatctaccga tttttaggaa acatcatgaa acgcacttat caaccttccg
    17041 ttaccaaacg caaacgcacc cacggcttcc tcgtgcgctc caaaacacgc ggcggccgcg
    17101 cagtattggc ggcgcggcgc gccaaaggcc gcaaacgcct ggcagtgtaa ttttggacta
    17161 ccgcttcgga aggcagtacc gcttgttaaa aacggatgat ttttcatccg tttttgcatt
    17221 cagaaaccgc cgcagccgcg acctgctgca agtttcgcgt tcaaacggca acgggctgga
    17281 ccatccccgc atcggtctgg tgatcggcaa aaaaaccgcc aaacgcgcca acgaacgaaa
    17341 ttatatgaag cgcgttatcc gcgactggtt tagattgaac aaaaaccggc tgccgccgca
    17401 agatttcgtc gtgcgcgtcc accgtaaatt cgacaggtct accgcaaaac aggcaagggc
    17461 ggaactggca caactcatgt tcggcaaccc cgcaaccgga tgcgggaaac aggtatgatt
    17521 agaacggtac tctgcaggca aggttcagac ggcaacgggt ttcccataca aggaacatcc
    17581 cgatgaactt tctattgtcc aaactcctgc tgggactgat acggttctac caatattgca
    17641 tcagcccgct gattccgccg cgctgccgtt atacgccgac ctgttcgcaa tacgcggtcg
    17701 aagcggtcaa aaaatacggc gcattcaaag gcggccggct cgccatcaag cgcattgcac
    17761 gctgccaccc tttcggcgga cacggacacg accccgttcc ctgacccgac gcaatattca
    17821 aattgcacgc tttcctttta tttcccatcg gtttctatat aatgccgtct gaaacttcgg
    17881 gctggcggca cgaccgtcgg gtatgaagcc cgcccttatt ccccgtctat cggaacacgc
    17941 aacctgcggc atttccgacc attcaggaaa ctcttatgga ttttaaaaga ctcacggcgt
    18001 ttttcgccat cgcactggtg attatgatcg gatgggaaaa gatgttcccc actccgaagc
    18061 ccgtccccgc gccccaacag gcagcacaac aacaggccgt aaccgcttcc gccgaagccg
    18121 cgctcgcgcc cgcaacgccg attaccgtaa cgaccgacac ggttcaagcc gtcattgatg
    18181 aaaaaagcgg cgacctgcgc cggctgaccc tgctcaaata caaagcaacc ggcgacgaaa
    18241 ccaaaccgtt cgtcctgttt ggcgacggca aagaatacac ctacgtcgcc caatccgaac
    18301 ttttggacgc gcagggcaac aacattctaa aaggcatcgg ctttagcgca ccgaaaaaac
    18361 agtacagctt ggaaggcgac aaagttgaag tccgcctgag cgcgcctgaa acacgcggtc
    18421 tgaaaatcga caaagtttat actttcacca aaggcagcta tctggtcaac gtccgcttcg
    18481 acatcgccaa cggcagcggt caaaccgcca acctgagcgc ggactaccgc atcgtccgcg
    18541 accacagcga acccgagggt caaggctact ttacccactc ttacgtcggc cctgttgttt
    18601 atacccctga aggcaacttc caaaaagtca gcttctccga cttggacgac gatgccaaat
    18661 ccggcaaatc cgaggccgaa tacatccgca aaaccccgac cggctggctc ggcatgattg
    18721 aacaccactt catgtccacc tggatcctcc aacccaaagg cggacaaagc gtttgcgccg
    18781 ctggcgactg ccgtatcgac atcaaacgcc gcaacgacaa gctgtacagc accagcgtca
    18841 gcgttcccct tgccgccatc ccggccggcg cgaaagccga aaccgccgtc aacctgtatg
    18901 ccggcccgca gaccacatcc gtcatcgcaa acatcgccga caacctgcaa ctggccaaag
    18961 actacggcaa agtacactgg ttcgcctccc ccctcttttg gcttttgaac caactgcaca
    19021 acatcatcgg caactggggc tgggcgatta tcgttttaac catcatcgtc aaagccgtac
    19081 tgtatccatt gaccaacgcc tcttaccgct ctatggcgaa aatgcgtgcc gccgcaccca
    19141 aactgcaagc catcaaagag aaatacggcg acgaccgtat ggcgcaacaa caggcgatga
    19201 tgcagcttta cacagacgag aaaatcaacc cgctgggcgg ctgcctgcct atgctgttgc
    19261 aaatccccgt cttcatcgga ttgtattggg cattgttcgc ctccgtagaa ttgcgccagg
    19321 caccttggct gggttggatt accgacctca gccgcgccga cccctactac atcctgccca
    19381 tcattatggc ggcaacgatg ttcgcccaaa cttatctgaa cccgccgccg accgacccga
    19441 tgcaggcgaa aatgatgaaa atcatgccgt tggttttctc cgtcatgttc ttcttcttcc
    19501 ctgccggcct ggtattgtac tgggtagtca acaacctcct gaccatcgcc cagcaatggc
    19561 acatcaaccg cagcatcgaa aaacaacgcg cccaaggcga agtcgtttcc taaatgccgc
    19621 agcatgaaaa atgccgtctg aaacctgttc agacggcatt tttattgctc accccctatc
    19681 ggggcggaaa tcttcaacct gcatacatca caaaaatcgt cgggcgtttt ttcagattgg
    19741 gcatttcttt tctttttcgc cactgcacaa ccgtctggct gatgatttcc tgagtcggca
    19801 acgtcaaatc cgtagccgta cacaaacgcg tttcagaatg caggttttcc accgcatccg
    19861 ccaacaacac atcgttgcga tacggcgttt cgataaacag ctgcgtttca ttctgctggc
    19921 gcgaacgttg ttccaaagcc ttcaaactct gaatccgctc atttttttca gacggcaaat
    19981 aacccttaaa cgcaaagttc tgcccattcg cacccgaagc catcagcgcc agcaacaagc
    20041 tggacggacc gaccaaaggc cgtacttcaa aaccgtgttt atgcgccaat gccaccaaat
    20101 tcgcacccgg atcggcaaca gccgggcaac ccgcctcgct gacaatgccc atactgcgcc
    20161 cttcttgcaa aggtttcagc aattccggca aagtcttcaa atccgtgtgt tcgttcagcg
    20221 tttgcaaatt cagttcgcgg ataggcattg tcacgcccaa atgtttcaaa tgtgcgcgcg
    20281 ccgttttttc cgcttccacg acaaaatccg tcagcccgac aaccgctcgt tgttcatgcg
    20341 gcaacaggca cggcgtatcg ggcgtaccca aaggcgtagg aatcaaatac aaaacaggag
    20401 acaccattcc ctcactcatc agttaaaaat gccgtctgag cctttcagac ggcataaacg
    20461 ggcagttaca gaacctccac gccctcattt ttcaaaaaat caaccagacg gaaaaccggc
    20521 aaaccgatta aagcattcgg atcggtactc tcaatccgtt cgatcaacaa tgcacccaaa
    20581 gcctcgctct tcaacgcaca cgagcaataa accgcatcag gctcgcgctc caaatagcgg
    20641 aggatgtgca gctcgtccaa cttcctcatc acgaccaccg tcttatcgat atgccgctgc
    20701 atcctgcccg taaccgtatt tagcagaacg accgcgctgt aaaactcaat ctccctgccg
    20761 ctcaaatgca tcagcatctt ttgcgcgttg gcaaggttca tcggcttgcc ccactgcctg
    20821 ccgtcgcacc acgccacctg gtccgcaccg acaatcaacg cctcggggaa acgtccggcc
    20881 aacgacctcg ccttaccctc ggcaaggcgc aatgccgtct gaggggcgga ttcccccaac
    20941 atcggcgttt cgtcaaaatc gggggacgcc gcctgaaagg caatgccgag cctttccatc
    21001 tgttcgcggc ggaaaaccga actcgtaccc aaaatcaaag gcagttccaa acccatccca
    21061 tcctccttac cgttgaaaac acgcccgaag gggcagtaaa atccagccat gcgccgaaac
    21121 acggataccc gccttcggcg taccgcaaca tttttcttaa aaatattgac gttagaacat
    21181 ctaaattata tcatatcccg tttatgtcag accctaattt gattgacccg gaaatttttg
    21241 ccgccgaagg gcagaacctg caaggcagtt ttctgctgga agaattggat gaacgcgtca
    21301 gttcgcacga ttatcccgcc gacaggcaga ccaaaatatc gtttacactg accggcggtc
    21361 gcgaccggct gcaacgcctg ttcctcgacc tgaacgtcaa agccgatatg cccctgattt
    21421 gccagagatg tatcaaaccc atgccgttca tgcttgatga aagcagccgt atcgtcctgt
    21481 tttccaacga agagtccttg gacgaatcca tgcttgccga cgaagaactc gaaggcatac
    21541 tgattgaaaa agaactcgac gtgcgcacat tggtagaaga ccaaatcctg atgtccctgc
    21601 ccttttcgcc gcgacacgaa gactgcggcg acaatgggac actggaagaa gtcaatcggg
    21661 acaaacccaa cccctttgct gttttggcag gtttgaaaag cagttaatta ggacacagtt
    21721 tatttatcta ggagcttgaa atggccgttc aacaaaacaa aaaatcccct tccaaacgcg
    21781 gtatgcaccg ttcgcacgac gcgctgaccg cgcctgcact gtctgtcgac agcacaaccg
    21841 gcgaagtaca ccgcccgcac cacatctccc ccaacggtat gtaccgcggc cgcaaagtgg
    21901 tcaaagccaa aggcgaataa tccctattcg actgactgaa aaagccagaa cattgccatg
    21961 caattactgg ctttttttgc attggacgca ccatccgtcc aaactttcgc catacgtcaa
    22021 cacacagggg caaagcgttc cgtataatac cccgtgaaaa tattccaaaa gccccaacca
    22081 ccaaggaaat tccgatgaaa cagaaaatct ggtacaccta cgatgacatc caccgcgtca
    22141 tcaaagcatt ggcagaaaaa atccggaacg ccgacatcaa atacgatgcc atgattgcca
    22201 tcggcggcgg cggctttatt ccggcacgta tgctgcgctg ttttctggaa attccgattt
    22261 atgccgtaac caccgcctat tacgacagcg acaacgaagg acaggttaca gaagaagtca
    22321 aaaaagtcca atggctcgac cccgttcccg aagccctgcg gggcaaaaac gtccttgttg
    22381 tcgatgaagt ggacgacagc cgcgtaacca tggaattttg cctgacagag ctgcttaaag
    22441 aagacttcgg caccatcggc gtcgccgtac tgcacgaaaa aatcaaagcc aaagcaggta
    22501 aaatccccga aggcattccc tatttcagcg gcatcaccgt agaagactgg tggatcaact
    22561 atccatggga cgcactcgac atcgacgaac acaaccgcct tgccgaggcc ggccgaggct
    22621 gaccctttca gacggcatat tttccgaacc gatgccgtct gaagcccgca cgacccctgc
    22681 cgcagaccga aaacctaccg gagaaacccc tatgattaca ttggccgtag atgccatggg
    22741 cggcgatcaa ggacttgccg ttaccgtacc cggcgcaacc gcattcctcc aagcacaccc
    22801 cgatgtccgc ctgattatga ccggcgacga aacgcaactg cgccaagccc tgaccgcggc
    22861 aggcgcaccg atggaacgca tcgacatctg ccacaccgca caaatcgtcg gcatggacga
    22921 agcgccgcaa tccgccctga aaaacaaaaa agactcctcc atgcgcgtcg ccgtcaacca
    22981 agttaaagaa ggcaaagccc aagccgccgt atccgcaggc aacacgggtg cgctcatggc
    23041 aaccgcacgt ttcgtcctca aaaccattcc cggcatcgaa cgccccgcca tcgccaaatt
    23101 cctgccttcc gacagcgaac acgttgccct ggcgttggat ttgggtgcca acgttgactg
    23161 cacgcccgaa caactggttc aatttgccat catcggcagc gagctggtac atgcgctgca
    23221 tcctgaaaaa ggcagcccgc gtgtcgccct tttgaacgtt ggtacagaag acattaaagg
    23281 tacagatgcc gtcaaacaaa cctttaaact gttgagcagc agcaaactca acttcatcgg
    23341 caatatcgaa agcaacagcg ttttgtatgg cgaagccgat gtcgttgtcg cagacggatt
    23401 tgtcggcaac gttatgctca aaaccatcga aggcgcggtt aaattcatga gtggtgccat
    23461 ccgccgcgaa ttccaaagca acctgttcaa caaacttgcc gccgttgccg ccctacccgc
    23521 cctcaaaggg ttgaaaaaca aactcgaccc gcgcaaattc aacggggcca tcctgctcgg
    23581 gctgcgcggc atcgtgatta aaagccacgg cggcacagac gaaaccggtt tccgctatgc
    23641 cctcgaagaa gcctaccatg aagccaagtc tgccagcctt tctaaaatcg aacaaggcgt
    23701 agccgaacag ctcgccgcac tcgaagctgc caaagccgcc caaaacgaaa tcgctgccga
    23761 cttgtaaaga aacataccct atgccgtctg aaaaccaacc gtttcagacg gcatctgtcc
    23821 gcaccaaacc tgcgggtgcg gacggcgatg cgcctgtccg gcacttccca aatacttccc
    23881 tgtaaaataa ggaatatttg aaaaatgaag acattagaaa aacggatgaa agctctagac
    23941 aaacggatga tgaagttcgg aaaatccctt gaaggcaggc ttgatgcccg tctgattgaa
    24001 tccgcattgg attatattca ttattcggaa cgttttttgg cttttgaaat cctgtgtact
    24061 tatatcgaag atttcgatgt ccggctgacg gaacaagaat cccgggaaat ttcttttatc
    24121 aacaaggaat ttgagataga aagcacgtcc gattaaccaa taaagccaat gggttgataa
    24181 acatgaaaac atcgacggtc gtttttggcg gattttttat ggcagacaac ggagaacgaa
    24241 tccaaatccc cgttttggaa aatcctgaca ttagggaaat caatcacttt ttttccatat
    24301 caaattttga gaaaaaagcc ggcgtccttg ttttcagaat catccccgag ccggaatttg
    24361 gcgataccga attaactgtc tattttgaaa aaggatatta tttgccaatg attcaaacaa
    24421 ttttagaagg cggggatatt gaagtcaaaa accttaaaac ggaaaattat agtggaaata
    24481 caatggaaat ttggggagat tcctacccta ttgagcatat ctctaaaaat atttctacaa
    24541 ttcaaaatat aatttctgaa tttattatac aaaaacaaac accggcacca ataatttgat
    24601 ggaaaaccaa caatcaaaat gccgtctgaa gcatgtttgg ttttcagacg gcatttcctt
    24661 cgcttaaaac agcgtatcgg caaccccgcc ccgcccgtcc acggcaatct gcatctgaaa
    24721 accatctgta tcccaaacca cacccccatc cctgtttccg tcatttgcac cctgcccgta
    24781 ttgggcaatc atcggttttt cgcttacaat agccgaatct gaaccaactc tctaaaaagg
    24841 ccgttcccat gcagtatgca aaaatttccg gcacgggcag ctatcttccc gccaaccgcg
    24901 tcagcaatga cgaccttgcc caaaaggtag atacctctga cgagtggatt accgcgcgta
    24961 cgggcatcaa gttccgccat attgcagccg aaaatgagaa aaccagcgat cttgccgccg
    25021 aagcagcccg ccgcgcactg gatgcagccg gattagacag cggcgaaatc gatttaatta
    25081 ttgtggcgac ggcaacgccg gatatgcagt ttccttctac cgccaccatc gtccaacaaa
    25141 aactgggcat caccaacggc tgccccgcgt ttgacgtaca ggcggtgtgt gcgggcttta
    25201 tgtacgcgct gaccacggca aacgcctaca tcaaaagcgg catggcaaaa aacgcgctgg
    25261 tcatcggcgc ggaaaccttc agtcgcattg tagactggaa cgaccgcaca acctgcgtat
    25321 tgttcggcga cggtgcgggc gcggtggttt taagcgcgtc cgacaaaccg ggcatcatcc
    25381 acagcaaact caaagccgac ggcaattatc tgaaactctt aaacgtcccc gggcaaatcg
    25441 cctgcggcaa agtttccggt tcgccgtaca tttcgatgga cggtcccggc gtgttcaagt
    25501 ttgccgtcaa aatgctgtcc aaaatcgccg atgacgttat cgaagaagcc ggttacaccg
    25561 ccgctcaaat cgactggatc gtgccgcatc aggcaaaccg ccgcattatc gaatcgaccg
    25621 cgaaacattt aggtttgagt atggacaaag tcgtcctgac cgtccaagac cacggcaaca
    25681 catccgccgc atcgattccg ctggctttgg atacgggcat ccgcagcgga caaatcaaac
    25741 gcggtcaaaa cctgctgctc gaaggcatcg gcggcggttt cgcgtggggc gcggtgctgt
    25801 tgcaatattg aacccgatgc cgtctgaaac aggctttcag acggcatttc ccatatcatg
    25861 aagcggcagg ctttcttcaa actgatggcg tgtgcggcat ttctgtctgc cgtttcgctg
    25921 cgcctccccg tattgggcgc gtgttacgca atattgtccc tctatgcgtt tgcactttac
    25981 ggcatcgaca aacggcgtgc cgtgcgggga aaacgccgca ttcccgaaca ccgcctgctg
    26041 ctgcctgcct tgttcggcgg ttgggcgggc gcatacttgg gcagcaggat attcaggcat
    26101 aaaacggcga aaaagcgttt tgttgtgctg ttccgtctga ctgtttcggg caatgtcctg
    26161 gcgacctgca tcct
//
LOCUS       DAXHSF010000010        25975 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.010, whole genome shotgun sequence.
ACCESSION   DAXHSF010000010 DAXHSF010000000
VERSION     DAXHSF010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25975
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.010"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..808
                     /locus_tag="ACFM8S_000325"
     CDS             <1..808
                     /locus_tag="ACFM8S_000325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC6313454.1"
                     /translation="RYRTPNIKYIYQDNQGTAEARNTGIKNSNGKYIVFIDSDDFINC
                     QVLLDFLSKDDIDMPDVVFLNAVKYDKGSVSHFGEDYQPEKILKQSKVEVLKGLCRFR
                     KFPGSACNKIIKRELIIKEKLFFEKEVYAEDIEWSMRLFNAATTFSYLDGCYYYYRQG
                     RKDSKTGTVSEKSIKSLLYILEKNAEMEFDRDISSYLYSFLSYEYLVLLFIMTSKNIE
                     CDADIKRRAYHLRFMLLKSNKLIYKLIFPIITLFGVDITGRILKAIRGNI"
     gene            849..1379
                     /locus_tag="ACFM8S_000326"
     CDS             849..1379
                     /locus_tag="ACFM8S_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFC6313455.1"
                     /translation="MNAIRTFQNRTPEIHESCMIDEACVVIGEVSLAEDVSVWPCAVL
                     RGDVNSITVGARSNIQDGSVLHVSHKTAAKPEGSPLVIGEDVTVGHKVMLHGCRIGNR
                     VLVGMGSTVLDDAVIEDEVMIGAGSLVPPRKRLEGGYLYVGSPVRQVRVLTDEEKAFL
                     KYSAAHYVKLSKQYGM"
     gene            complement(1413..1529)
                     /locus_tag="ACFM8S_000327"
     CDS             complement(1413..1529)
                     /locus_tag="ACFM8S_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor domain protein"
                     /protein_id="HFC6313456.1"
                     /translation="MPSEPEKRASDGIFACNSNADGQNQGASDTVIGCVPSA"
     gene            complement(1548..3143)
                     /locus_tag="ACFM8S_000328"
     CDS             complement(1548..3143)
                     /locus_tag="ACFM8S_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFC6313457.1"
                     /translation="MSQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGT
                     VKGKKTGKFATSDWMDIEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDAAKGVEAQTIKLLNVCRLRDTPIVTFMNKYDREVRDSLELLDEVENI
                     LQIRCAPVTWPIGMGKNFKGVYHILNDEIYLFEAGGERLPHEFDIIKGIDNPELEQRF
                     PLEIQQLRDEIELVQAASNEFNLDEFLAGELTPVFFGSAINNFGIQEILNSLIDWAPA
                     PKPRDATVRMVEPDEAKFSGFIFKIQANMDPKHRDRIAFLRVCSGKFERGMKMKHLRI
                     NREIAASSVVTFMSHDRELVEEAYAGDIIGIPNHGNIQIGDSFSEGEQLAFTGIPFFA
                     PELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSR
                     LANEYGVEAVFDSASIWSARWVSCDDKKKLAEFEKANAGNLAIDAGGNLAYLAPNRVN
                     LGLTQERWPDIVFHETREHSVKL"
     gene            complement(3250..3645)
                     /gene="hisI"
                     /locus_tag="ACFM8S_000329"
     CDS             complement(3250..3645)
                     /gene="hisI"
                     /locus_tag="ACFM8S_000329"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HFC6313458.1"
                     /translation="MDKNLLEAVKFDEKGLVCAIAQDAETKRVLMVAWMNAEALQKTV
                     ETGFAHYYSRSRQKQWMKGEESGHTQKVRELRLDCDGDAIVMLIAQNGGIACHTGRES
                     CFYKVWRGSAWETADAVLKDEKEIYGSTH"
     gene            complement(3676..4443)
                     /gene="hisF"
                     /locus_tag="ACFM8S_000330"
     CDS             complement(3676..4443)
                     /gene="hisF"
                     /locus_tag="ACFM8S_000330"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFC6313459.1"
                     /translation="MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGDPVEAAKRYNGEG
                     ADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTVGGGVRTVADIRRLLNAGADKVS
                     INTAAVTRPDLIDEAAGFFGSQAIVAAVDAKAVNSENTRWEIFTHGGRNPTGLDAVEW
                     AIEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGI
                     TEGKADAVLAAGIFHFGEIAIREAKRAMREAGIEVRL"
     gene            complement(4456..5193)
                     /gene="hisA"
                     /locus_tag="ACFM8S_000331"
     CDS             complement(4456..5193)
                     /gene="hisA"
                     /locus_tag="ACFM8S_000331"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFC6313460.1"
                     /translation="MLLIPAIDLKEGRCVRLKQGLMEEATVFSDSPAETALHWFKQGA
                     RRLHLVDLNGAFAGVPQNLPAIKDILAAVAKDIPVQLGGGIRDLKTIGQYLDLGLNDV
                     IIGTAAVKNPDFVREACKAFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDD
                     GVNSIIYTDIGRDGMMSGVNIDATVKLAQAVRIPVIASGGLTGLDDIRALCAAEKHGV
                     AGAITGRAIYEGSIDFAQAQQLADSLD"
     gene            complement(5226..5864)
                     /gene="hisH"
                     /locus_tag="ACFM8S_000332"
     CDS             complement(5226..5864)
                     /gene="hisH"
                     /locus_tag="ACFM8S_000332"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFC6313461.1"
                     /translation="MQTAIIDYGMGNLHSVLKSVRTAGQLAGKNTEIFLSGDPDRVSR
                     ADKVIFPGQGAMPDCMAALTRGGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDG
                     LGWFGGKVRRFERDLRDPQGCRLKVPHMGWNTVRQTQNHPLFQGIPQDTRFYFVHSYY
                     FAPENPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW"
     gene            complement(5995..7497)
                     /gene="pta"
                     /locus_tag="ACFM8S_000333"
     CDS             complement(5995..7497)
                     /gene="pta"
                     /locus_tag="ACFM8S_000333"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFC6313462.1"
                     /translation="MAKVLIVPVSAGLDASAAAQAFAKALDAEIFQAVDATAETLLAQ
                     GKSDDWFDALVGKVAALDAANLVIEGIAPDADKIYLAGKNVELALSLDAAAVFAVRSD
                     NADADELANRLNLAKQFFAAAPGVLEGFVVDGAAASVAEAAAEKTGLTFFGSSDALKD
                     VSVLAGREAKRLSPAQFRYNLIDFARQADKRIVLPEGAEPRTVQAAAICHEKGIARCV
                     LLAKREEVEAVAKERGISLPDSLEIIDPASLVEQYVEPMCELRKSKGLTPEDARKQLQ
                     DTVVLGTMMMAQNDVDGLVSGAVHTTANTIRPALQLIKTAPGASLVSSVFFMLLPNQV
                     LVFGDCAVNPNPTAQQLADIAIQSADSAKAFGIDPKVAMISYSTVNSGSGPDVDTVIE
                     ATKLAREKRPDLAIDGPLQYDAATVPGVGKSKAPGSPVAGQATVLVFPDLNTGNCTYK
                     AVQRSANVLSVGPLLQGLRKPVNDLSRGALVEDIVFTIALTAVQAKQMEG"
     gene            complement(7682..8740)
                     /gene="fbpC"
                     /locus_tag="ACFM8S_000334"
     CDS             complement(7682..8740)
                     /gene="fbpC"
                     /locus_tag="ACFM8S_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein FbpC"
                     /protein_id="HFC6313463.1"
                     /translation="MTAALHIGHLSKSFQNTPVLNDISLSLDPGEILFIVGASGCGKT
                     TLLRCLAGFEQPDFGEISLSGRTIFSKNTNLPVRERRLGYVVQEGVLFPHLTVYRNTA
                     YGLGNGKGKTAQERQRIEAMLELTGISELAGRYPHELSGGQQQRVALARALAPDPELI
                     LLDEPFSALDEQLRRQIREDMIAALRANGKSAVFVSHDREEALQYADRIAVMKQGRIL
                     QTASPHELYRQPADLDAALFIGEGIVFPAALNADGTADCGLGRLPVQSGAPAGTRGTL
                     LIRPEQFSLHPHSAPTASIHAVVLKTTPKARHTEISLRVGQTVLTLNLPSAPTLSDGI
                     SAVLHLDGPALFFPGNTL"
     gene            complement(8761..10278)
                     /gene="fbpB"
                     /locus_tag="ACFM8S_000335"
     CDS             complement(8761..10278)
                     /gene="fbpB"
                     /locus_tag="ACFM8S_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease FbpB"
                     /protein_id="HFC6313464.1"
                     /translation="MSPKKIPIWLTGLILLIALPLTLPFLYVAMRSWQVGINRAVELL
                     FRPRMWDLLSNTLTMMAGVTLISIVLGIACALLFQRYRFFGKTFFQTAITLPLCIPAF
                     VSCFTWISLTFRVEGFWGTVMIMSLSSFPLAYLPVEAALKRISLSYEEVSLSLGKSRL
                     QTFFSAILPQLKPAIGSSVLLIALHMLVEFGAVSILNYPTFTTAIFQEYEMSYNNNTA
                     ALLSAVLMAVCGIVVFGESIFRGKAKIYHSGKGVARPYPVKTLKLPGQIGAIVFLSSL
                     LTLGIIIPFGVLIHWMMVGTSGTFALVSVFDAFIRSLSVSALGAILTILCALPLVWAS
                     VRYRNFLTVWIDRLPFLLHAVPGLVIALSLVYFSINYTPAVYQTFIVVILAYFMLYLP
                     MAQTTLRTSLEQLPKGMEQVGATLGRGHFFIFRTLVLPSILPGITAAFALVFLKLMKE
                     LTATLLLTTDDVHTLSTAVWEYTSDAQYAAATPYALMLVLFSGIPVFLLKKYAFK"
     gene            complement(10346..11341)
                     /gene="fbpA"
                     /locus_tag="ACFM8S_000336"
     CDS             complement(10346..11341)
                     /gene="fbpA"
                     /locus_tag="ACFM8S_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     FbpA"
                     /protein_id="HFC6313465.1"
                     /translation="MKTSIRYALLAAALTAATPALADITVYNGQHKEAAQAVADAFTR
                     ATGIKVKLNSAKGDQLAGQIKEEGSRSPADVFYSEQIPALATLSAANLLEPLPASTIN
                     ETRGKGVPVAAKKDWVALSGRSRVVVYDTRKLSEKDLEKSVLNYATPKWKNRIGYAPT
                     SGAFLEQVVAIVKLKGEAAALKWLKGLKEYGKPYAKNSVALQAVENGEIDAALINNYY
                     WHAFAREKGVQNVHTRLNFVRHRDPGALVTYSGAAVLKSSQNKDEAKKFVAFLASKEG
                     QRALTAVRAEYPLNPHVVSTFNLEPIAKLEAPQVSATTVSEKEHATRLLEQAGMK"
     gene            complement(11592..12062)
                     /locus_tag="ACFM8S_000337"
     CDS             complement(11592..12062)
                     /locus_tag="ACFM8S_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
                     /protein_id="HFC6313466.1"
                     /translation="MTDTDTQADRFEQMMWQAVDKLFEQHDGKLESMDGREQELVLIW
                     RAEADIGNGGILQFVCNWGFPAAEKTCSVLKKIGAIHSAMLIHRAADALRKEIRHLQS
                     EGKNLKEMWDITKHLDNKTASLPNSLDEQYWQDPDKLYLPGWQYYSGNPVQTLF"
     gene            complement(12117..13493)
                     /gene="argH"
                     /locus_tag="ACFM8S_000338"
     CDS             complement(12117..13493)
                     /gene="argH"
                     /locus_tag="ACFM8S_000338"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFC6313467.1"
                     /translation="MHDKTWSGRFNEPVSELVKQYTASIGFDRRLAEWDIQGSLAHAQ
                     MLKETGVLDEGDLADIRRGMAEILEEIRSGKIEWSSDLEDVHMNIERRLTDKIGDAGK
                     RLHTGRSRNDQVATDIRLWLRDQITVIRSLIQSLQTALLDLAEQNAETVMPGFTHLQV
                     AQPVSFGHHMLAYVEMLGRDNERMADCRRRVNRMPLGAAALAGTTYPIRREITAELLG
                     FEQICQNSLDAVSDRDFAVEFTAAASLIMVHLSRLSEELILWMSPRFGFIDIADRFCT
                     GSSIMPQKKNPDVPELVRGKSGRVIGHLIGLITLMKSQPLAYNKDNQEDKEPLFDTAD
                     TLIDTLRIYADMMRGVTVKPDNMRAAVMQGFATATDLADYLVKKGMPFRDAHEVVAQA
                     VRHADEAGVDLSELPLEVLQGFSDLIADDVYGVLTPEGSLNARNHLGGTAPEQVRLQV
                     KRWREVSA"
     gene            complement(13512..14381)
                     /gene="galU"
                     /locus_tag="ACFM8S_000339"
     CDS             complement(13512..14381)
                     /gene="galU"
                     /locus_tag="ACFM8S_000339"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFC6313468.1"
                     /translation="MKPIKKAVFPVAGMGTRFLPATKASPKEMLPIVDKPLIQYAVEE
                     AVEAGCTEMVFVTGRNKRSIEDHFDKAYELETELEMRHKDKLLEHVRNILPPNITCLY
                     IRQAEALGLGHAVLCARAAIGDEPFAVILADDLIDAPKGALKQMVEVYGRSGNSILGV
                     ETVEASQTGSYGIVETEQLKQFQRIIGIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     GLPRGAGNEIQLTDGIAKLLDHEFVLAHPFEGTRYDCGSKLGYLEATVAYGLKHPETG
                     EPFRRLLEKYRTE"
     gene            complement(14409..15008)
                     /gene="rdgB"
                     /locus_tag="ACFM8S_000340"
     CDS             complement(14409..15008)
                     /gene="rdgB"
                     /locus_tag="ACFM8S_000340"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HFC6313469.1"
                     /translation="MSEKPEKIVLASGNAGKLEEFGNLFKPYSITVLPQSAFGIPECP
                     EPYPTFVENALAKARHAAEYSGLPALADDSGICAAALNGAPGIHSARYAGDHPKSDTA
                     NNLKLAAELVGKADKSCCYVCVLVFVRHKDDPRPIIAEGVWHGQWNGVPAGENGFGYD
                     PYFYLPGHGKTAAELDSEVKNRESHRAQALADLIRKLAL"
     gene            complement(15001..15234)
                     /locus_tag="ACFM8S_000341"
     CDS             complement(15001..15234)
                     /locus_tag="ACFM8S_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGO_0222 family membrane protein"
                     /protein_id="HFC6313470.1"
                     /translation="MNRRKIYLLSVALFTLAFMLLVLLGAYLLTAGSKAFAVASFFFA
                     FGALFGQIGSLALYLRHKSLRAAQSAAKENRYV"
     gene            complement(15342..15875)
                     /locus_tag="ACFM8S_000342"
     CDS             complement(15342..15875)
                     /locus_tag="ACFM8S_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFC6313471.1"
                     /translation="MADFNQILTPGDVDGGIINVVNEIPAGSNHKIEWNRKLAAFQLD
                     RVEPAIFAKPTNYGFIPQTLDEDGDELDVLLVTEQPLATGVFLEARVIGVMKFVDDGE
                     VDDKIVCVPADDRNNGNAYKTLSDLPQQLIKQIEFHFNHYKDLKKAGTTKVESWGDAE
                     EAKKVIKESIERWNKQA"
     gene            complement(15977..16435)
                     /gene="nudB"
                     /locus_tag="ACFM8S_000343"
     CDS             complement(15977..16435)
                     /gene="nudB"
                     /locus_tag="ACFM8S_000343"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFC6313472.1"
                     /translation="MPMAKPLKYPVSALVVLYSGDGGILLIERTHPKGFWQSVTGSLE
                     PGETVAQTARREVWEETGILLADGQLQDWHDSTVYEIYHHWRHRYPKGVFENREHLFS
                     AEIPRDTPVVLQPEEHVSYGWFGLEEAAEKVFSPSNRRAILELGRFLGKR"
     gene            16646..16722
                     /locus_tag="ACFM8S_000344"
     tRNA            16646..16722
                     /locus_tag="ACFM8S_000344"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16680..16682,aa:Pro,seq:ggg)
     gene            16880..18706
                     /gene="mafB"
                     /locus_tag="ACFM8S_000345"
     CDS             16880..18706
                     /gene="mafB"
                     /locus_tag="ACFM8S_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
                     /protein_id="HFC6313473.1"
                     /translation="MGISRKISLILSILAVCLPMHAHASDLANDSFIRQVLDRQHFEP
                     DGKYHLFGSRGELAERSGHIGLGKIQSHQLGNLMIQQAAIKGNIGYIVRFSDHGHEVH
                     SPFDNHASHSDSDEAGSPVDGFSLYRIHWDGYEHHPADGYDGPQGGGYPAPKGARDIY
                     SYDIKGVAQNIRLNLTDNRSTGQRLADRFHNAGSMLTQGVGDGFKRATRYSPELDRSG
                     NAAEAFNGTADIVKNIIGAAGEIVGAGDAVQGISEGSNIAVMHGLGLLSTENKMARIN
                     DLADMAQLKDYAAAAIRDWAVQNPNAAQGIEAVSNIFMAAIPIKGIGAVRGKYGLGGI
                     TAHPIKRSQMGAIALPKGKSAVSDNFADAAYAKYPSPYHSRNIRSNLEQRYGKENITS
                     STVPPSNGKNVKLADQRHPKTGVPFDGKGFPNFEKHVKYDTKLDIQELSGGGIPKAKP
                     VSDAKPRWEVDRKLNKLTTREQVEKNVQEIRNGNKNSNFSQHAQLEREINKLKSADEI
                     NFADGMGKFTDSMNDKAFSRLVKSVKENGFTNPVVEYVEINGKAYIVRGNNRVFAAEY
                     LGRIHELKFKKVDFPVPNTSWKNPTDVLNESGNVKRPRYRSK"
     gene            18709..19173
                     /locus_tag="ACFM8S_000346"
     CDS             18709..19173
                     /locus_tag="ACFM8S_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313474.1"
                     /translation="MAIWNYRYYLIPSAAIRNKFNANKDIILDEYRSNGFQNFNENKS
                     FENYFIDNDVILLSIINEAKKQLKLKESWDKDAIMFCDNFGNSLTVWPDDIECELDLR
                     FDYTKFIQKTIDWAIKYNCLLVIEKTGNVVSPNINNLMYEIKAYLESKPWPI"
     gene            19398..19544
                     /locus_tag="ACFM8S_000347"
     CDS             19398..19544
                     /locus_tag="ACFM8S_000347"
                     /inference="COORDINATES: protein motif:HMM:NF012755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313475.1"
                     /translation="MWYEANIGLINTMSRSNQAGTRLLYSNYGLLYITTDHYISATRF
                     VAWK"
     gene            19556..19843
                     /locus_tag="ACFM8S_000348"
     CDS             19556..19843
                     /locus_tag="ACFM8S_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFC6313476.1"
                     /translation="MQLEIIGSKIYTEQDFHNQISKIFSIQDYYGNNLDALWDLLSTN
                     VERPITLVWKDAMFSKNQLENIFIEIVNVLERVKKQDEDYGFEEKFNYILE"
     gene            20357..21595
                     /locus_tag="ACFM8S_000349"
     CDS             20357..21595
                     /locus_tag="ACFM8S_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
                     /protein_id="HFC6313477.1"
                     /translation="MIASLTGAAVGGTPVDAQTGGAVGQNAVENNLYLTSEALKKDKQ
                     TARKIYSVIKEQVKHECSSTGRITECRQNIGRIIEFTQDKRFDSRFKDLKKESLYYLN
                     KHPDLVASYLKAEYEKLDREDKSILHRYISPGAEIVSGSLGVVLSGVAGGGSCAETFG
                     LGCAAALVGVTSSYDHVITGTKNFGKKASEQRPTIAVQALKQLGLSEQAAEYVQFSID
                     LFSVGKSGGGIPKAKPVFDAKPRWEVDRKLNKLTTREQVEKNVQETRRRSQSSQFKAH
                     AQREWENKTGLDFNHFIGGDINKKGTVTGGHSLTRGDVRVIQQTSAPDKHGVYQATVE
                     IKKPDGSWEVKTKKGGKVMTKHTMFPKDWDEARIRAEVTSAWESRIMLKDNKWQGTSK
                     SGIKIEGFTEPNRTAYPIYE"
     gene            21626..22045
                     /locus_tag="ACFM8S_000350"
     CDS             21626..22045
                     /locus_tag="ACFM8S_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313478.1"
                     /translation="MKRIKCFCDKFPSGDTFRMCIILDDYDNRVDYYVGIYDYITSTL
                     MSDIYYRSTIDEHFKIIELIENNPNEIYDDGGGQQFCLEFHHDKVIFYHNEFDEEDGY
                     PVLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            22082..23056
                     /gene="mafB"
                     /locus_tag="ACFM8S_000351"
                     /pseudo
     CDS             22082..23056
                     /gene="mafB"
                     /locus_tag="ACFM8S_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
     gene            23067..23528
                     /locus_tag="ACFM8S_000352"
     CDS             23067..23528
                     /locus_tag="ACFM8S_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313479.1"
                     /translation="MNLETAENIQCEFEYLALDGYFPMHFASHGQGNKDWQFAVEFIY
                     RLLICQLATLEPINFETKNDILDFCHNLAKQSPFNNDNEVWYQGEIVLTKKGIDLIKE
                     YIPEAFEEWNGKKFELNIPFIKTLKNIFVNYEVAWDENNPLFPIISLNLGT"
     gene            23515..23802
                     /locus_tag="ACFM8S_000353"
                     /pseudo
     CDS             23515..23802
                     /locus_tag="ACFM8S_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229180.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
     gene            23829..24248
                     /gene="mafI"
                     /locus_tag="ACFM8S_000354"
     CDS             23829..24248
                     /gene="mafI"
                     /locus_tag="ACFM8S_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MafI"
                     /protein_id="HFC6313480.1"
                     /translation="MYKIKCFYDQFSSGELFNYCQFLDNSSNQKINTRGTVYQYIIYV
                     LTGDLYLQKDIDENLEFIHQAENNPNEVYSGGGQGFCWDISAEKVVFYHNEFDEEDGW
                     PDLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            24338..24409
                     /locus_tag="ACFM8S_000355"
                     /pseudo
     CDS             24338..24409
                     /locus_tag="ACFM8S_000355"
                     /inference="COORDINATES: protein motif:HMM:NF026862.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin type 17 domain-containing
                     protein"
     gene            24406..24648
                     /gene="imm31"
                     /locus_tag="ACFM8S_000356"
     CDS             24406..24648
                     /gene="imm31"
                     /locus_tag="ACFM8S_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm31 family immunity protein"
                     /protein_id="HFC6313481.1"
                     /translation="MTNLKLDFYSEVIIKDSCPNDLLENGETIKGKKGVVLGISEEDG
                     IIYGYTILLFDIKYCIYIDKKYIIPTGKKFSRDDFY"
     gene            24726..24971
                     /locus_tag="ACFM8S_000357"
     CDS             24726..24971
                     /locus_tag="ACFM8S_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC6313482.1"
                     /translation="MKDSTGQMRLATKDLAEAIKRGEVRSSAFTTKQLKAIEKGESKI
                     PDYTWHHHQDTGRMQLINEDLHHDTGHIGWRAMSKGK"
     gene            24974..25364
                     /locus_tag="ACFM8S_000358"
                     /pseudo
     CDS             24974..25364
                     /locus_tag="ACFM8S_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            25465..25854
                     /locus_tag="ACFM8S_000359"
     CDS             25465..25854
                     /locus_tag="ACFM8S_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313483.1"
                     /translation="MWKITKEQGQDLSFATYCLLSAAINLQEFKLWLEKVVLDMPIDN
                     IHFYIFDLIDLKEGVGDIYNILDFVPNSDLSKDQDDALTGIAFLRGIDVYDPPVSKEK
                     ALKALKKHPEIYQRFQHFFPFVELPPL"
BASE COUNT         6532 a         5700 c         7162 g         6581 t
ORIGIN      
        1 cagataccga acaccaaaca tcaaatatat atatcaggat aaccaaggga cggcagaagc
       61 taggaatacc ggaataaaaa attcaaacgg aaaatatata gtctttattg attcggatga
      121 ttttattaat tgtcaggtct tgctggattt tcttagtaaa gatgatattg atatgccgga
      181 tgtggttttt ttaaatgcgg ttaaatatga taagggaagt gtttcacatt ttggcgaaga
      241 ttatcagcct gaaaaaatac tcaagcaatc caaagtcgaa gttttgaaag gattatgccg
      301 atttagaaaa tttccgggtt cggcgtgtaa taagattata aaaagggaat tgattatcaa
      361 agaaaaactg ttttttgaaa aagaggttta tgccgaagat atcgaatggt caatgagatt
      421 atttaatgcg gcaacaactt tttcttattt ggacggttgt tattactatt atcggcaggg
      481 aagaaaagat tctaaaacag gaactgtttc ggaaaaaagt ataaagtcat tattatatat
      541 tttggagaaa aatgcggaaa tggaatttga tagggatata tcaagttatc tttattcttt
      601 tctttcctac gaatatctcg ttttgctttt tatcatgacg agtaaaaata tagagtgtga
      661 tgctgatata aaaagaaggg cgtatcattt aaggtttatg ctgttaaagt ccaataaatt
      721 gatatataag ctgatattcc cgataatcac attattcggg gttgatatta caggcaggat
      781 tttaaaagca atcagaggga atatttaata aatcctttaa cactatacat cttaccgaag
      841 gagaaaaaat gaacgcaatc cgaactttcc aaaaccgcac gcccgaaatc cacgaaagct
      901 gtatgataga cgaagcgtgc gtcgtcatcg gcgaagtgtc gcttgccgaa gacgtttccg
      961 tgtggccgtg cgccgtgttg cgcggcgatg tgaacagcat caccgtcggc gcgcgcagca
     1021 atatacagga cggcagcgtc ctgcacgttt cccacaaaac cgccgccaaa cccgaaggat
     1081 cgccgctggt tatcggcgaa gacgttaccg tggggcacaa agtgatgctg cacggctgcc
     1141 gaatcggcaa ccgcgtcttg gtcggtatgg gcagcaccgt tttggacgat gctgtgattg
     1201 aggacgaagt gatgatcggc gcgggcagcc tcgtgcctcc gcgcaaacgt ttggagggcg
     1261 gctatcttta tgtcggttcg ccggtcagac aagtgcgcgt gttgaccgat gaggaaaaag
     1321 cctttttgaa gtattccgcc gcgcattatg tgaagctgtc gaaacagtac gggatgtgaa
     1381 atcacatcgg cgttcttgcg tcagccccaa attcatgcgg atgggacgca tccgataacg
     1441 gtatccgatg cgccttgatt ttgaccgtct gcgtttgagt tgcaggcaaa aatgccgtct
     1501 gaagcccttt tttcgggttc agacggcatt ttattgccga ttgcttttta aagtttgacc
     1561 gaatgctcgc gcgtttcgtg gaacacgatg tccggccagc gttcttgcgt gagtcccaga
     1621 ttcacgcggt taggggcgag gtaggcgagg ttgccgcccg cgtcgatggc gaggttgcct
     1681 gcattggctt tttcaaattc cgccagtttt ttcttgtcgt cgcacgatac ccagcgcgcc
     1741 gaccagatgg atgcgctgtc gaacacggct tctacgccgt attcgttggc cagccgcgag
     1801 gtaacgactt caaattgcaa cacgccgacc gcgcccaaaa tcaaatccgc gccgctcatc
     1861 ggtttgaaca cctgcaccgc accttcttcg ccaagctgtt gcaaaccttt ttgcagttgc
     1921 ttgattttca gcgggttttt gatgcgaacg ctgcggaaca gttcgggcgc gaagaaggga
     1981 atgcctgtaa atgccagttg ttcgccttcg gagaagctgt cgccgatttg gatgttgccg
     2041 tggttcggga tgccgatgat gtcgccggcg taggcttctt ccaccagctc gcggtcgtgc
     2101 gacatgaagg ttaccacgct ggaggcggcg atttcgcggt tgatgcgcag gtgtttcatc
     2161 ttcatgccgc gctcgaattt gccggagcag acgcgcaaaa aagcgatgcg gtcgcggtgt
     2221 ttcggatcca tattggcttg gattttaaag ataaatccgg aaaacttagc ctcgtccggt
     2281 tcgaccatac gcacggtcgc gtcgcgcggt ttcggcgcgg gcgcccagtc aatcaatgaa
     2341 ttgaggattt cctgaatacc gaagttgtta atcgccgaac cgaaaaacac gggcgtgagt
     2401 tcgccggcga gaaattcgtc gagattaaac tcgttcgatg ctgcctgcac taattcgatt
     2461 tcgtcgcgca actgttggat ttccaacgga aagcgttgtt ccaattcagg attatcgata
     2521 cctttgatga tgtcgaattc gtgcggcaga cgttcgccgc cggcttcaaa gagatagatt
     2581 tcatcgttca ggatgtggta tacgcctttg aagtttttgc ccataccaat cggccaggta
     2641 acgggcgcgc agcggatttg caggatgttt tccacttcgt ccaagagttc cagcgagtcg
     2701 cgcacttcgc ggtcgtattt gttcataaac gtaacaatcg gcgtatcgcg caggcggcag
     2761 acgtttaaga gcttgatggt ttgcgcttcc acaccttttg ccgcgtcaat caccattaat
     2821 gcgctgtcca cggcggttaa aacgcggtag gtgtcttcgg agaagtcttg gtgtcccggc
     2881 gtgtccaaga ggttgacggt gtggtcttta tagtcgaact gcatcacgct tgatgccacg
     2941 gaaatgccgc gctgcttctc gatgtccatc cagtcggagg tggcgaattt gccggttttc
     3001 ttgcctttta ccgtacccgc gctttgaatc gcacctgaaa acagcaagag tttttcagtc
     3061 agcgtggttt tacctgcgtc agggtgggag atgatggcaa acgtgcggcg gcggcgcact
     3121 tggtcgagga tttcttggga catggttttc tttgcaaaaa ggttcaggcc gcttttcaga
     3181 cggcccggac agtgtttgag acggcgaaat tgtacaaaaa aatgcctgat aattcaatgt
     3241 tggaggcggt cagtgcgtgc tgccgtaaat ctctttttcg tctttcagga cggcatcggc
     3301 ggtttcccac gcgctgccac gccagacttt gtaaaagcag ctttctcgcc cggtgtggca
     3361 ggcgatgccg ccgttttggg cgatgagcat cacaatggcg tcgccgtcgc agtcgaggcg
     3421 cagttcgcgg actttttgcg tgtgtcccga ctcttcgccc ttcatccatt gtttttggcg
     3481 cgaacggctg taatagtggg caaagccggt ttcgacggtt ttttgcaggg cttcggcgtt
     3541 catccacgcc accattaaaa cacgtttggt ttcggcatct tgggcgatgg cgcaaaccaa
     3601 acctttttcg tcaaatttga cggcttcaag caggttttta tccatatttc ctttcagacg
     3661 gcatagtcgg agcggtcaga ggcgcacttc gatgccggct tcgcgcatag cgcgtttggc
     3721 ttcgcggatg gcgatttccc cgaaatggaa aatgccggcg gcaagtacgg catcggcttt
     3781 gccttcggtt ataccttcaa tcaggtgccg gacattgccg acccctccgg aggcgatgac
     3841 ggggatgtcg acggcttcgg caacggcgcg ggtcagcggc aggttgaaac cctgtttcgt
     3901 accgtccctg tccatcccgg tgagcaggat ttcgcccgcg ccgcgttttt gcatttcgat
     3961 cgcccattcc accgcatcca aaccggtcgg attgcgcccg ccgtgggtaa agatttccca
     4021 gcgtgtgttt tcggagttga cggctttggc atcgacggcg gcgacgatgg cttgcgaacc
     4081 gaaaaatccg gcggcttcgt caatcaggtc gggacgggta acggcggcgg tgttgatgct
     4141 gactttgtcc gcgccggcat tgagcaggcg gcggatgtcg gcaacggtgc gtacgccgcc
     4201 gccgacggtc agggggatga agacttgtcc ggcaacctct tcgatgatgt gcaggatggt
     4261 gtcgcggttg tcggatgagg cggtgatgtc gaggaaggtc aattcgtccg cgccttcgcc
     4321 gttgtagcgt ttggcggctt cgacggggtc gcccgcgtcg cgcaaaccga tgaagttcac
     4381 gcctttgacg acgcgcccgt cttttacgtc gagacagggg atgatgcgtt ttgccagtgc
     4441 cataatcgga tgcctttagt cgagggaatc tgccagttgc tgcgcttggg caaaatcgat
     4501 gctgccctcg taaatcgcgc ggccggtaat cgcgcctgct acgccatgtt tttcggcggc
     4561 acacagggcg cggatgtcgt ccaagccggt cagtccgccg gaggcgatga cgggaatgcg
     4621 gacggcttgg gcgagtttga ccgtcgcgtc gatgttcacg ccgctcatca taccgtcgcg
     4681 cccgatgtcg gtgtagatga tgctgttgac gccgtcgtct tcaaagcgtt ttgccaaatc
     4741 aattacatga tgcccggtta cggttgccca gccgtcgatg gcggccatac cgtctttggc
     4801 atccagcccg acaataatcc tgccggggaa ggctttgcac gcctcgcgca cgaagtcggg
     4861 gtttttgacc gccgccgtgc cgataatcac gtcgtttaag cccaaatcca aatattgtcc
     4921 gatggttttc aaatcgcgta tgccgccgcc gagctgtacg gggatgtctt tggcgacagc
     4981 ggcaaggatg tctttgatgg cgggcaggtt ttgcggaacg ccggcaaacg cgccgttcaa
     5041 atctaccaga tgcaggcggc gcgcgccttg tttgaaccag tgcagcgcgg tttcggcggg
     5101 cgaatcggaa aagacggtcg cctcttccat cagcccttgt ttcaggcgga cgcagcgtcc
     5161 ttctttcaaa tcgatggcgg gtatcagcag cataattttt ctccttgtgc ggggccgtgt
     5221 ccggcttacc agtttaaaaa gtttttcaac atcgtcagcc cggcatcgtg gcttttttcg
     5281 gtgtgaaatt gcgtggcgaa tacgttgtct ttgccgacga tgcaggcaaa cggggacggg
     5341 tagtcgcttt cgcccaatat ggtttcggga ttttcggggg cgaaatagta gctgtggacg
     5401 aagtaaaaac gcgtgtcttg ggggatgcct tgaaaaagcg ggtggttttg ggtttggcgc
     5461 acggtgttcc agcccatatg cgggactttc agacggcatc cctgcgggtc gcggaggtcg
     5521 cgctcaaagc gtctgacttt gccgccgaac cagcccaagc cgtcggtgtt tccttcttcg
     5581 ctgtggtcga ataaaagttg cgcgccgacg cagattccga aaaagggttt gttttttaag
     5641 gcatctttga ctgcctcgtc caagccgccg cgcgtcagtg ccgccataca gtcgggcatc
     5701 gcgccctgac cgggaaaaat gactttgtcg gcgcgggaca cgcggtcggg gtcgccgctt
     5761 aaaaagattt cggtattttt tccggcaagc tgccccgccg tccggacgga tttcaatacg
     5821 gaatgcaggt tgcccatacc gtaatcgata atggcggttt gcatggcttc ctcctctttt
     5881 tttgcaatat ggctgcgatt ttaacaaaca aatgtgccgg gctgataaaa atgccgtctg
     5941 aaaacgggag tctgtcttca gacggcatag ggtttaaccc cggaaagccg tttgtcagcc
     6001 ttccatttgt tttgcctgaa cggcagtcag ggcgatggtg aacacgatgt cttccaccag
     6061 cgcgccgcgc gacaggtcgt tgaccggttt gcgcaggcct tgcagcagcg ggccgacgct
     6121 taagacgttg gcactgcgtt ggacggcttt ataggtgcag ttgccggtgt tcaggtcggg
     6181 gaagaccaaa acggttgcct gtcctgccac cgggctgccc ggggctttgg atttgcccac
     6241 gcccggcacg gttgccgcat catattgcag cgggccgtcg atggcgaggt cggggcgttt
     6301 ttcccgggca agtttggttg cttcgatgac ggtatcgaca tcggggccgc tgccggagtt
     6361 gacggtggag taggaaatca tcgccacttt cgggtcgatg ccgaaggctt ttgcggaatc
     6421 ggcagactgg atggcgatgt cggcaagctg ttgcgcggtc gggttcggat taaccgcgca
     6481 gtcgccgaag acgaggactt ggttgggcag cagcataaag aatacgctgg acacgaggct
     6541 tgcgcccggt gcggttttaa tcagttgcaa agcggggcgg atggtgttgg cggtggtgtg
     6601 aaccgcaccg gataccaaac cgtccacatc attttgcgcc atcatcatcg taccgagtac
     6661 cacggtgtct tgcagttgct tgcgcgcgtc ttcgggtgtc aggcctttgg atttgcgcag
     6721 ttcgcacatt ggctcgacgt attgttcgac caatgaggcg ggatcgatga tttccaaaga
     6781 gtcgggcagg ctgatgccgc gttctttggc aacggcttcg acttcttcgc gtttggcaag
     6841 caggacgcag cgggcaatgc ctttttcgtg gcagatggcg gcggcttgga cggtgcgggg
     6901 ttctgcgccc tcaggcagga cgatgcgttt gtcggcttgg cgggcgaagt cgatcaggtt
     6961 gtagcggaat tgcgccggcg acaggcgttt tgcttcgcgg cctgccaata cggatacgtc
     7021 tttcagcgcg tcgctcgaac cgaagaaggt caggccggtt ttttcggctg ccgcttcggc
     7081 aacggaggct gccgcgccgt ccacgacaaa accttccaat acgcccggcg cggcggcgaa
     7141 gaactgtttg gcaaggttca gccgatttgc cagttcgtcg gcatcggcgt tgtcggaacg
     7201 gacggcgaag acggctgccg cgtcaaggga caatgccagt tcgacgtttt tgcctgcgag
     7261 gtagattttg tcggcatcgg gcgcgatgcc ttcgatgacg aggttggcgg catcgagtgc
     7321 ggcaactttg ccgaccagtg cgtcgaacca gtcgtcgctt ttgccttgcg cgagcagggt
     7381 ttcggcggtt gcgtcaacgg cttggaaaat ttctgcgtcc agtgcttttg caaaggcttg
     7441 tgcggcggcg gaggcgtcca gtccggcaga tacgggtacg atgagtactt ttgccatgat
     7501 atatcctttc gtatgctgca gtgtgcggca tatgtggttg gaaggggcgg catataggca
     7561 gaaacggctg cctgcgtgcc gtgcgtgccg tgtttggctt gaggcgcgca ggttgaatat
     7621 agcaaacaaa ttctgtttcc aacaagataa atatccgcag gcttgtggat gctgccgcct
     7681 ttcagagggt atttccgggg aagaacaggg cgggaccgtc caaatggagg acggcggaaa
     7741 tgccgtctga cagggtgggg gcggaaggga ggttgagcgt gaggacggtt tgtccgaccc
     7801 ggaggctgat ttcggtatgc cgcgctttgg gcgtggtttt gagaaccacg gcgtgaatgg
     7861 aggcggtggg tgcggaatgg gggtgaaggc tgaactgttc cggacggatg agcagtgtgc
     7921 cgcgtgtgcc tgcgggtgcg ccgctttgaa caggcaggcg gcccaatccg caatcggcgg
     7981 tgccgtcggc gttgagcgcg gcggggaaca cgatgccttc gccgataaac agggcggcat
     8041 caaggtcggc aggttgtcgg tacaattcgt gagggcttgc agtttggagg atgcgcccct
     8101 gtttcatcac ggcaatccgg tcggcgtatt gcagggcttc ttcgcggtcg tggctgacga
     8161 aaacggcaga tttgccgttg gcgcgcaggg cggcaatcat gtcttcgcga atctggcggc
     8221 gcaactgttc gtccagcgcg ctgaagggtt cgtccaacaa aatcagttcg ggatcgggtg
     8281 cgagggcgcg ggcgagggcg acgcgctgtt gctgtccgcc cgaaagttcg tgcggatagc
     8341 gtccggcaag ttcggaaatg ccggtcaatt ccaacatagc ttcgatgcgc tgccgctctt
     8401 gcgccgtctt gcctttgccg ttgcccagcc cgtaggcggt gttgcggtaa acggtcaggt
     8461 gggggaacag tacgccttcc tgtacgacat aacccaaacg gcgttcgcgg acggggaggt
     8521 tggtattttt cgagaagatg gttctgccgg aaagcgaaat ttcgccaaaa tcgggttgtt
     8581 caaaaccggc aaggcagcgt aaaagggtgg ttttgccgca gccggacgcg ccgacgataa
     8641 agaggatttc gcccgggtcg aggctgagcg aaatgtcgtt taaaaccggg gtgttttgaa
     8701 aacttttgga caggtgtccg atgtgcaggg cggcggtcat ggcggtactt cctcaagctg
     8761 ttatttgaag gcgtatttct tcagcaggaa tacggggatg ccggaaaata ataccagcat
     8821 cagcgcgtaa ggggtggcgg cggcgtattg tgcgtccgat gtgtattccc aaacggcggt
     8881 ggagagtgtg tggacatcgt cggtggtcag cagcagggtg gcggtcagct ctttcatcag
     8941 tttgaggaag acgagtgcga atgcggcggt aatgccgggc aggatggacg gcagtaccaa
     9001 cgtcctgaaa ataaagaagt gtccgcgccc caatgttgcg ccgacctgtt ccatcccttt
     9061 ggggagttgt tccaaggaag tcctcagggt ggtttgcgcc atcggcaggt aaagcatgaa
     9121 ataggcaagg atgacgacga taaaggtttg gtaaacggca ggggtgtagt tgatgctgaa
     9181 ataaaccaag gatagggcga taaccaaacc ggggacggcg tgcagtaaaa acggcagcct
     9241 gtctatccaa acggttaaaa aattgcgata gcgaaccgat gcccaaacaa ggggcaaggc
     9301 acataatata gtcaaaatcg cacctaaagc cgatacgctt aaggaacgga taaaggcatc
     9361 aaatacggat acgagcgcga atgtgccgga agtgccgacc atcatccaat gtatcaatac
     9421 gccaaagggg ataataatgc ccaaagtcaa caagctgctt aaaaaaacaa tcgcgccaat
     9481 ctgaccgggc agtttgaggg ttttgacggg ataaggacgg gcaacgcctt tgccgctgtg
     9541 gtaaatcttg gctttgccgc gaaatatgct ttctccaaat acgacgatgc cgcacaccgc
     9601 cattaaaaca gcggaaagca gggcggcggt attgttgttg taggacattt cgtattcttg
     9661 gaaaatggcg gtggtaaaag tggggtagtt caaaatagat accgcgccaa attcgaccag
     9721 catatgcagg gcaatcagta acacgctgct gccgatggcg ggtttgagct gggggaggat
     9781 ggcggaaaaa aaggtttgca ggcggctttt gcccaaggac aggctgactt cttcgtaaga
     9841 caggctgatg cgtttgagtg ccgcctcgac gggcaggtag gcgagcggga acgaggacag
     9901 gctcataatc atcactgtcc cccaaaagcc ttcgacacgg aaggtcaggc tgatccaggt
     9961 gaaacagctg acaaatgcgg ggatgcacaa aggcagggtg attgccgtct gaaaaaaggt
    10021 tttgccgaag aagcggtaac gttggaacaa aagggcgcag gcaatgccca aaacaatgga
    10081 aatcagggta acgcccgcca tcatcgtcaa ggtgttggag agcaaatccc acatacgcgg
    10141 gcggaacaac agttcgacgg cgcggttgat gccgacctgc cacgaacgca tagcgacata
    10201 taaaaaaggc agggtaagcg gtagggcaat cagtaggatg aggccggtaa gccaaatggg
    10261 tattttttta ggagacatag tgttttttat cggcaaaacg ggcggacagt ataaatgtcc
    10321 acccgtttga caatccgaaa acggcttatt tcataccggc ttgctcaagc agccgggtgg
    10381 cgtgttcttt ttcggaaaca gtggtggcgg acacttgggg tgcttccaac ttggcgatgg
    10441 gttccaaatt gaaagtggat accacgtgcg gattcaaagg atattcggca cggacggcgg
    10501 tcagggcgcg ctgtccttcc ttgctggcga ggaaggcgac gaattttttc gcctcatcct
    10561 tgttttggga ggattttaac acggctgcgc cggaataggt aacgagtgcg ccgggatctc
    10621 tgtggcggac gaaattcagg cgggtgtgga cattttgtac gcctttttca cgcgcaaaag
    10681 cgtgccagta gtagttgttg atgagggcgg catcgatttc gccgttttca accgcttgaa
    10741 gggcgacgga gtttttagcg taaggcttgc cgtattcttt cagacctttg agccatttca
    10801 atgcggccgc ttcgcctttc agtttgacga tggcgacaac ctgttccaag aacgcgccgg
    10861 aagtgggggc gtaaccgatg cggtttttcc atttcggcgt ggcgtaattc aggacggatt
    10921 tttccaaatc tttttcagac agtttgcggg tgtcgtaaac gacgacgcgc gaacgtccgc
    10981 tcagtgccac ccagtctttt ttggcggcaa ccggcacgcc cttgccgcgt gtttcgttga
    11041 tggtggaggc gggcaggggc tctaggaggt tggctgcgga aagggtggcg agtgccggga
    11101 tttgttcgga atagaatacg tcggcggggc ttcggctgcc ttcttctttg atttgaccgg
    11161 caagctggtc gcctttggcg ctgttgagtt tgactttgat gccggtagcc cgggtaaagg
    11221 catctgcaac ggcttgtgcc gcttctttgt gttggccgtt gtacacggta atgtctgcca
    11281 gcgcgggggt tgcggcggtc agggctgcgg caagcagtgc gtatcggata gatgttttca
    11341 tatcgatttt ctcctaatga atgagagtgt acaccttgtt aagacataac ggggtgtagt
    11401 gtattccttc ttctttataa atgcaaataa ttatttttta aatttgttat tatccgatcc
    11461 ggttattgtt tgttctgact tgtatttttt ccgtgcatcg cgcccgtaag gcggaagcgg
    11521 cgggcaatgc gtggcggaat gtgggtaaag gcggcatttt gatttgtcgg aatgcttgag
    11581 aacccctctc tttaaaacag cgtctgaaca gggttgccgg aatagtattg ccatcccggc
    11641 agatacagtt tgtcggggtc ttgccaatat tgttcatcca gactgttcgg cagtgaggcg
    11701 gttttgttgt cgagatgttt tgttatatcc cacatttctt tcaggttttt accttccgat
    11761 tggaggtggc ggatttcttt tctcaatgcg tctgccgccc gatggatcag cattgcgctg
    11821 tgtatcgcgc cgattttttt cagaacggaa caagtctttt cggcggcggg aaaaccccaa
    11881 ttgcagacaa attgcagtat gccgccgttg ccgatgtcgg cttccgcccg ccaaatcaaa
    11941 accagctcct gttcccgccc gtccatgctt tcgagtttgc cgtcatgctg ttcaaaaagt
    12001 ttgtccaccg cctgccacat catctgttcg aagcggtcgg cttgggtatc cgtatcggtc
    12061 atagtgttct tgccttttaa aaatgccgtc tgaacggctt cagacggcat cgggggttaa
    12121 gctgacactt cacgccagcg tttgacttgg aggcgtacct gttcaggcgc ggtgccgccc
    12181 aagtggttgc gcgcgtttaa gctgccttcg ggtgtcagca cgccgtaaac gtcgtcggca
    12241 atcaaatcgc tgaaaccttg taagacttcg agcggcagtt cgctcaaatc gacgccagct
    12301 tcatcggcgt ggcgcacggc ttgggcgacg acttcgtggg catcgcggaa gggcatacct
    12361 tttttaacca gataatccgc caagtcggtg gcggtggcaa aaccctgcat cacggcggcg
    12421 cgcatattgt cgggtttgac ggttacgccg cgcatcatat cggcgtaaat ccgcagcgtg
    12481 tcgatgagcg tgtcggcggt gtcaaacaaa ggttctttgt cttcctgatt gtctttgttg
    12541 tacgccaagg gctgggattt catcagggta atcaggccga taaggtgtcc gatgacgcgt
    12601 ccggatttgc cgcgcacgag ttcgggcacg tcgggatttt tcttctgcgg catgatggac
    12661 gaacctgtgc agaaacggtc ggcgatgtcg ataaagccga aacgcggact catccacaaa
    12721 atcaattctt cggacaggcg gctcaggtga accataatca gcgaggcggc ggcggtgaac
    12781 tcgacggcaa aatcgcggtc ggatacggca tcgagcgagt tttggcagat ttgctcgaaa
    12841 cccagtaatt cggcggtaat ttcgcgccga atcggataag tcgtaccggc aagggcggct
    12901 gcgccgagcg gcatacggtt gacgcggcgg cggcagtccg ccatccgttc gttatcgcgt
    12961 ccgagcattt cgacgtaggc gagcatatgg tgtccgaagc tgacgggctg ggcgacttgc
    13021 aggtgggtaa agcctggcat gacggtttcg gcgttttgtt ccgccaaatc cagcaatgcc
    13081 gtctgaaggc tttgaatcag gcttcggatg acggtaatct ggtcgcgcag ccaaaggcgg
    13141 atgtcggtgg cgacttggtc gttgcggctg cggccggtgt gcaggcgttt gcccgcgtcg
    13201 ccgattttgt cggtcaggcg gcgttcgatg ttcatgtgga catcttccaa atcggacgac
    13261 cattcgattt tgccgctgcg gatttcttcg aggatttccg ccatgccccg acggatgtcc
    13321 gccaaatcgc cttcgtccaa cacgccggtt tctttcaaca tttgcgcgtg tgccagcgag
    13381 ccttgaatgt cccattcggc aagccgtcgg tcgaaaccga tggaggcggt gtattgtttg
    13441 acgagttcgg aaacgggttc gttgaaacgt ccggaccagg ttttgtcgtg cataaggatt
    13501 ccttgatggg gttattcggt gcggtatttt tccaaaaggc ggcggaaggg ttcgccggtt
    13561 tcggggtgtt tcaggccgta ggcgacggtg gcttcgaggt agcccagttt gctgccgcag
    13621 tcgtagcgcg tgccttcaaa ggggtgcgcc aggacaaatt cgtgatcgag cagcttggcg
    13681 atgccgtctg taagctggat ttcgttgccc gcgccgcgcg gcagtccggt cagtaagtcg
    13741 aaaatgcgcg gggtaaggat gtagcgtccg acgacggcaa ggttggaggg cgcgtcttcg
    13801 ggcttgggtt tttcgacaat gccgataatg cgttggaact gtttgagctg ttcggtttcg
    13861 acgatgccgt atgagccggt ttgcgatgct tcaacggttt ctacgcccaa aatgctgttg
    13921 ccgctgcgcc cgtacacttc gaccatttgt ttgagcgcgc ctttgggggc atcaatcagg
    13981 tcgtcggcaa ggataacggc aaagggttcg tctccgatgg cggcgcgggc gcacaagacg
    14041 gcgtgtccca agcccagtgc ttccgcctga cggatgtaga ggcaggtaat gttcggtggc
    14101 aggatgttgc ggacgtgttc cagaagtttg tctttatggc gcatttccaa ctcggtttcg
    14161 agttcgtatg ccttgtcgaa atggtcttcg atgctgcgtt tgttgcgtcc ggtcacaaac
    14221 accatttccg tgcagccggc ttccacggct tcttctacgg cgtattggat cagcggcttg
    14281 tcgacgatgg gcagcatttc tttcgggctt gccttggtgg cgggcaggaa gcgggttccc
    14341 atccctgcga cggggaagac ggcttttttt atcggtttca ttctttttcc tttgtattgt
    14401 tttgatgttt aaagggcgag tttgcggatt aaatcggcga gtgcctgcgc gcggtggctt
    14461 tcgcggtttt tgacctccga atccaattcg gcggcggttt taccgtgtcc gggcagataa
    14521 aaatacgggt cgtaaccgaa accgttttca ccggcgggaa cgccgttcca ttgtccgtgc
    14581 catacgcctt cggcaataat cggacgcggg tcgtctttat ggcggacaaa aaccaatacg
    14641 cagacgtagc aacagctttt gtcggctttg ccgacaagtt cggcggcaag tttcaggttg
    14701 ttggcggtat cggatttggg atggtcgccc gcgtaacgtg cggaatggat gcccggcgcg
    14761 ccgtttaggg cggcggcaca gatgccgctg tcgtcggcaa gcgcgggcag cccgctgtat
    14821 tcggcggcat ggcgtgcttt tgccagcgcg ttttcgacaa aggtgggata gggttcgggg
    14881 cattcgggta tgccgaatgc ggattgcggc aatacggtga tgctgtaagg tttgaataag
    14941 ttgccgaatt cttcgagctt gccggcattg ccgcttgcca aaacgatttt ttccggtttt
    15001 tcagacatag cggttttcct ttgcggcgga ttgggcggcg cgtagggatt tgtgccgcag
    15061 gtagagggcg aggctgccga tttgtccgaa cagtgcgccg aatgcgaaaa aaaaggaggc
    15121 gacggcgaag gctttgctgc cggcggtcag cagataagca cccaagagga cgagcagcat
    15181 aaatgccagt gtgaagaggg caacagacaa cagatagatt tttcggcggt tcatggcgtt
    15241 cggtcggaaa cggtatgttc ggattatatc cgattgggac ggtattccct agagcttgga
    15301 aaaatgccgt ctgaagcggc gttcagacgg catggcgggt gttatgcctg tttgttccaa
    15361 cgttcgatgg attctttaat gacttttttc gcttcttccg catcgcccca cgattcgact
    15421 ttggtcgtac ctgctttttt caggtctttg taatggttga agtggaactc gatttgtttg
    15481 atgagctgtt gcggcaaatc ggacaaagtt ttgtaggcgt tgccgttgtt gcggtcgtcg
    15541 gcaggaacgc agacgatttt gtcgtccact tcgccgtcgt caacgaattt catcacgccg
    15601 ataacgcgcg cttccaagaa tacgccggtt gccaaaggtt gttcggtaac gagcagcacg
    15661 tccaattcgt cgccgtcttc gtccaaagtt tggggaatga agccgtagtt ggtcggtttg
    15721 gcgaagatgg cgggttcgac gcggtcgagt tggaatgcgg cgagtttgcg gttccactcg
    15781 attttgtggt tgctgccggc ggggatttcg ttgacgacgt tgatgatgcc gccgtccaca
    15841 tcgccggggg tcaggatttg gttgaagtct gccatttggt ttcctttgtt tgggaaagtt
    15901 tgaagtttga aagtatagca caaacgtccg gctgaaaatg cgcccgatgc cgtctgaaag
    15961 ggtgtacggg cgcgtgttac cgtttgccca aaaacctgcc cagttccaga atcgcgcgcc
    16021 tgttggacgg ggaaaacact ttttccgccg cttcttccaa gccgaaccag ccgtaggaga
    16081 cgtgttcctc gggttgcagg acgacgggcg tatcgcgcgg gatttcggca gagaagaggt
    16141 gttcgcggtt ttcaaacacg ccttttggat agcggtgccg ccagtggtgg tagatttcgt
    16201 aaaccgtgct gtcgtgccag tcttgaagct gcccgtccgc cagcaggatg ccggtttctt
    16261 cccaaacttc gcgccttgcc gtttgggcga cggtttcgcc cggttcgagg ctgccggtta
    16321 ccgactgcca aaatcctttc ggatgcgtgc gttcgatgag caggatgccg ccgtccccgc
    16381 tataaaggac gaccagtgcg gaaacggggt atttgagcgg ttttgccatc ggcatctttc
    16441 ggcgggctgc ggtaatgaag gggcggatta tagcaaacgc cgcacgttat ggcgtttatc
    16501 cttttccgta tccttttttc tgcacggatg ggacgcgccg gtgtttgccg gtaaattttc
    16561 cgttgtgtca aaaagataag ggcggttgtg attttaatgc ttgccaaagc gtcgggcgga
    16621 aactataatc cgaaacttat cgagtcggag tgtggcgcag tctggtagcg cacttgcatg
    16681 gggtgcaagg ggtcgaaggt tcgaatcctt tcactccgac caaaaattcc gaaagccgct
    16741 ttcaaaagcg gcttttttgc cgtccgtatg attatgatgt agagtacgcg gcgacagaca
    16801 ttcaaatgcc gtctgaaaac cgttcagatg gcatctcttt atcttagttt cattccgtac
    16861 catcttaagg aacatcaaat tgggcatttc ccgcaaaata tcccttattc tgtccatact
    16921 ggcagtgtgc ctgccgatgc atgcacacgc ctcagatttg gcaaacgatt cttttatccg
    16981 gcaggttctc gaccgtcagc atttcgaacc cgacgggaaa taccacctat tcggcagcag
    17041 gggggaactt gccgagcgca gcggccatat cggattggga aaaatacaaa gccatcagtt
    17101 gggcaacctg atgattcaac aggcggccat taaaggaaat atcggctaca ttgtccgctt
    17161 ttccgatcac gggcacgaag tccattcccc cttcgacaac catgcctcac attccgattc
    17221 tgatgaagcc ggtagtcccg ttgacggatt tagcctttac cgcatccatt gggacggata
    17281 cgaacaccat cccgccgacg gctatgacgg gccacagggc ggcggctatc ccgctcccaa
    17341 aggcgcgagg gatatataca gctacgacat aaaaggcgtt gcccaaaata tccgcctcaa
    17401 cctgaccgac aaccgcagca ccggacaacg gcttgccgac cgtttccaca atgccggtag
    17461 tatgctgacg caaggagtag gcgacggatt caaacgcgcc acccgataca gccccgagct
    17521 ggacagatcg ggcaatgccg ccgaagcctt caacggcact gcagatatcg ttaaaaacat
    17581 catcggcgcg gcaggagaaa ttgtcggcgc aggcgatgcc gtgcagggca taagcgaagg
    17641 ctcaaacatt gctgtcatgc acggcttggg tctgctttcc accgaaaaca agatggcgcg
    17701 catcaacgat ttggcagata tggcgcaact caaagactat gccgcagcag ccatccgcga
    17761 ttgggcagtc caaaacccca atgccgcaca aggcatagaa gccgtcagca atatctttat
    17821 ggcagccatc cccatcaaag ggattggagc tgttcgggga aaatacggct tgggcggcat
    17881 cacggcacat cctatcaagc ggtcgcagat gggcgcgatc gcattgccga aagggaaatc
    17941 cgccgtcagc gacaattttg ccgatgcggc atacgccaaa tacccgtccc cttaccattc
    18001 ccgaaatatc cgttcaaact tggagcagcg ttacggcaaa gaaaacatca cctcctcaac
    18061 cgtgccgccg tcaaacggca aaaatgtcaa actggcagac caacgccacc cgaagacagg
    18121 cgtaccgttt gacggtaaag ggtttccgaa ttttgagaag cacgtgaaat atgatacgaa
    18181 gctcgatatt caagaattat cggggggcgg tatacctaag gctaagcctg tgtctgatgc
    18241 aaaaccgaga tgggaggttg ataggaagct taataaattg acaactcgtg agcaggtgga
    18301 gaaaaatgtt caggaaataa ggaacggtaa taaaaacagt aactttagcc aacatgctca
    18361 actagagagg gaaattaata aactaaaatc tgccgatgaa attaattttg cagatggaat
    18421 gggaaaattt accgatagca tgaatgacaa ggcttttagt aggcttgtga aatcagttaa
    18481 agagaatggc ttcacaaatc cagttgtgga gtacgttgaa ataaatggaa aagcatatat
    18541 cgtaagagga aataataggg tttttgctgc agaatacctt ggcaggatac atgaattaaa
    18601 atttaaaaaa gttgactttc ctgttcctaa tactagttgg aaaaatccta ctgatgtctt
    18661 gaatgaatca ggtaatgtta agagacctcg ttataggagt aaataaaaat ggcaatttgg
    18721 aattatcggt actaccttat cccatcagct gctatcagaa ataaatttaa tgcaaataaa
    18781 gatatcatac ttgatgagta tcgatctaat ggttttcaga attttaatga gaataaaagt
    18841 tttgaaaatt actttatcga taatgatgtt atattattat caataataaa tgaagcaaaa
    18901 aaacagctta aattgaaaga atcttgggat aaagacgcaa tcatgttttg tgataatttt
    18961 ggtaatagtc ttaccgtttg gccagatgat atagagtgcg aacttgattt aagatttgat
    19021 tatactaaat ttattcagaa aaccattgat tgggcaataa aatataattg tctacttgta
    19081 atagaaaaaa caggaaatgt agtttcccct aatataaata atctgatgta tgaaataaaa
    19141 gcatatttgg aaagcaagcc gtggcccata tgaaacctaa actcaacaag taggatgtgt
    19201 gcggaacgca cgtatgcggt tctcaaggtt tgagctaaga ggccgtctga aaacagaaaa
    19261 actgtttcag acgacctttc ttttaaccag ttgccacagc aaccggacaa aagcagccta
    19321 cctccacatc catataggca atacagggga gatattttgt aaattctacg aatattttac
    19381 ctgctaaaca gggtaggata tggtatgaag cgaacattgg cttaataaac actatgtcaa
    19441 gatcgaatca ggctggtact agattgttgt attccaatta tggattgcta tatataacaa
    19501 ctgatcatta tatctctgca acacggtttg tagcttggaa ataggagtat aacttatgca
    19561 attagagatt atcggtagta aaatttatac ggaacaagat tttcataatc aaatttcaaa
    19621 aatattttct atacaagatt attatgggaa caatcttgat gctttatggg atttattaag
    19681 cacaaatgta gaacgaccga ttactttggt atggaaagat gctatgttct caaaaaatca
    19741 attagaaaat atatttattg aaatcgtaaa tgttctagaa agagttaaga aacaagatga
    19801 ggattatgga ttcgaagaaa aatttaatta tattttagag taagtaaacc ctaattacat
    19861 tacgtacaca ggcttaaaac tccccagagc caattaagca agccgtaacc catataaaac
    19921 ttaaactcaa caagtagcat gtgtgcggaa cgtacgcatg cggttcttaa agtttgagct
    19981 aagaggccgt ctaaaaacag aaaaaccgtt tcagacggtc tttgtttaac gccaccgatc
    20041 cagcgggtta caaagcgcag tcaatgccgc tgcgccttat gcctccgaag caataggcag
    20101 aacatttgga cacggtgaaa acaaaaacga aaccgcccaa gccgtcggac atttcctttt
    20161 aggagcagct attgcccgcg tcaacggtgg taattttgct gccggcggct cggcagcagt
    20221 tgcagctgaa aaggcggcgg aacatcttgc ccaacagtat aacgacggta aaaccgcaat
    20281 cgatccgcaa acaggcgagt tcaatgccaa cctgctgccg gaacatatca aagaggaaat
    20341 caaatcaaag agcggggtga ttgcatcgct gacgggcgcg gccgtgggcg gcacgccggt
    20401 agatgcgcaa accggaggtg cggtcggaca gaatgcggtg gaaaacaacc tctatctgac
    20461 atcggaagcc ttaaagaagg acaagcagac agctcgtaaa atttattccg tcataaaaga
    20521 gcaagtcaag catgaatgca gttccacagg aagaattacc gaatgtcgtc aaaatatagg
    20581 acgcattatc gaatttaccc aagacaaacg ctttgacagt aggtttaagg acttaaaaaa
    20641 agaatcctta tattacctaa ataaacatcc tgatttagta gcctcttatt tgaaggctga
    20701 atacgaaaag ctggataggg aagacaaaag tatcctgcac cgctacatct cacccggggc
    20761 tgaaatcgtt tcgggcagtt tgggggttgt tctttcagga gtagccggag gcggatcttg
    20821 tgccgagact ttcggcttag gctgtgccgc cgctttggtt ggtgtaacgt cttcctacga
    20881 tcatgtcatt actgggacga aaaacttcgg aaaaaaagcc agcgagcaac gaccgacgat
    20941 tgcggttcag gccttgaagc agttggggct gtcggagcag gctgcggaat atgttcagtt
    21001 ctctatagat ttgttcagtg tgggtaaatc ggggggcggt atacctaagg ctaagcctgt
    21061 gtttgatgcg aaaccgagat gggaggttga taggaagctt aataaattga caactcgtga
    21121 gcaggtggag aaaaatgttc aggaaacgag aagaaggagt cagagtagtc agtttaaagc
    21181 ccatgcgcaa cgagaatggg aaaataaaac agggttagat tttaatcatt ttataggtgg
    21241 tgatatcaat aagaaaggca cagtaacagg agggcatagt ctaacccgtg gtgatgtacg
    21301 ggtgatacaa caaacctcgg cacctgataa acatggggtt tatcaagcga cagtggaaat
    21361 taaaaagcct gatggaagtt gggaggtgaa aacgaaaaaa ggtgggaaag tgatgaccaa
    21421 gcacaccatg ttcccaaaag attgggatga ggctagaatt agggctgaag ttacttcggc
    21481 ttgggaaagt agaataatgc ttaaggataa taaatggcag ggtacaagta aatcgggtat
    21541 taaaatagaa ggatttaccg aacctaatag aacagcatat cccatttatg aatagtaata
    21601 tttatgaaaa attaggagat taatgatgaa aagaattaag tgcttttgtg ataaatttcc
    21661 atcaggagat acatttagaa tgtgtatcat tctggatgac tatgataata gggttgatta
    21721 ttatgtagga atatatgatt acattacgtc taccttaatg agcgatattt actatcgatc
    21781 cacgattgat gagcatttca agattataga attaatagaa aataatccaa atgaaattta
    21841 tgatgatggc ggtggtcaac aattttgcct agaatttcat catgataagg tcatttttta
    21901 tcacaatgaa tttgatgaag aagatggtta tccagtatta agctgttcgc tgcatacttt
    21961 taaaactgct ttaattgctt ggaatgcttt tttgcaattg cctaaaagta ttcattcggt
    22021 ggtggagact gtgattgagg aataagcata attagcttaa tgaatagaat cagcgatata
    22081 gaaaggtaga atcagatata tcccacctaa aaattactct ccttcagcac cgctaccaaa
    22141 aggacctaat aatggatata gtgccgggaa tttaagcaaa aaagtcctga ctttagttta
    22201 cataaacggc aaactcaaat tcgggccaag cagtctgccg tcttcggcat tgaaagcacc
    22261 tgttcccatt gtcggcaagg ctgaggctcc gcccggaaaa gctgtgcctg tcggcgaagc
    22321 ccgcattcct tcaaatccaa aagcggtaaa aggaacaacc gttaccctgt cgcaggaaca
    22381 acgcctagcc aacggagagc tgataccgaa aggctctgtg gtaacgtggt cggacaatgc
    22441 ggtgaaggtt gtgcggcctg atgggaaaaa gcggagattt aatagtcggg attgggaaca
    22501 gaaggcattg ccgaaaccgc aactaccgaa agattacaga gatgacatca acataggtgg
    22561 aaataatcct agatttaatc agcaacggca tgaaaaaatc gaatcccaca acccaaacag
    22621 aatagaggag atacgccaac gtaatgccgt ttcttctgca gaaaggcagg ctgatgcatt
    22681 aaaaaggatg caaacgtctg gccgcacccg cctgcctggg cagccacaaa aaggaaaacc
    22741 cggcgctgct agagaaatgt cctcttcaga caatccgaat caaacagccg aggcttttgc
    22801 acgtcgatat ttaggcagga aacctacact aatggaacga gtcgaaggtg aaaaattata
    22861 cggaggtaaa tgtgtgggct gttggaaagc gcaaatgccg gacggaacct ttgtggtgta
    22921 taggccagca ggtaatgctt ctatgaccaa gggaactact gcgtctgttg atattaataa
    22981 cccaacatta aataacttaa atattaattt aaaaggcaag ccaacagagt taaaatttaa
    23041 attccctgct agataaggat gttttaatga atttagagac agcagaaaat attcaatgcg
    23101 aatttgagta tttagcatta gatggttatt ttccaatgca ttttgcatcg catggtcaag
    23161 gaaacaaaga ttggcaattt gcagttgaat tcatatatag attattaata tgtcaattag
    23221 caactcttga acctatcaac tttgaaacga aaaatgatat tttagacttt tgccataatt
    23281 tagcaaaaca aagtcctttt aataatgata atgaggtgtg gtatcagggg gaaattgtat
    23341 taacaaaaaa agggatagat ttaataaagg aatatattcc agaggcgttt gaagagtgga
    23401 atggaaagaa atttgaatta aacattcctt tcattaaaac attaaaaaat atatttgtta
    23461 attatgaggt agcatgggat gagaataatc cactattccc cattatatct ttaaacctcg
    23521 gcacctgata aacatggggt ttatcaagcg acagtggaaa ttaaaaagcc tgatggaagt
    23581 tgggaaatga aaaaaggaag gggcggaaaa cctatgacaa atcattctat gttcccaaaa
    23641 gattgggatg aggctagaat tagggctgaa gttacttcgg cttgggagaa aagaaaggaa
    23701 ttagaaggtc aaaaatggag aggtacaagt aaatctggtg ttgaaataga aggatttacg
    23761 gaacctaatc gaacagctta tccactttat aatagtaaat gaagtattga ttaaaaaggt
    23821 gtttttaaat gtataaaata aaatgttttt atgatcagtt tagttctggt gaattattta
    23881 actactgcca gtttttagat aattcttcta atcaaaagat aaatacacga ggaacggtgt
    23941 atcaatatat tatttatgtt ttaacaggag acctttatct acaaaaagac atagatgaaa
    24001 atttagaatt tatccatcaa gcagaaaata atcccaatga agtttattca ggaggtggac
    24061 aaggtttttg ctgggatatt tctgctgaaa aagtagtttt ctatcacaac gaatttgatg
    24121 aagaagacgg ctggcctgat ttaagctgtt cgctgcatac ttttaaaact gctttaattg
    24181 cttggaatgc ttttttgcaa ttgcctaaaa gtattcattc ggtggtggag actgtgattg
    24241 aggaataagc ataattagct taatgaatag aatcagcgat atagattgga ctgcaaatcc
    24301 acgcttatac gctgtgccat gattaacagg aagagagcaa cttggatggg ctagtaggga
    24361 tggtaagcat ttaaatatat caattgatgg aaagattaca cacaaatgac taatttaaaa
    24421 ttagatttct actctgaagt tattataaaa gattcttgcc ctaatgattt gttagaaaat
    24481 ggtgagacta ttaaaggaaa aaaaggagtg gtattaggta taagcgaaga agatggtata
    24541 atctatggct atactatttt actttttgat ataaaatatt gtatatatat agataagaaa
    24601 tatattatac cgactggaaa aaaattctct cgcgatgatt tttattagct aatttaagta
    24661 atttaagcaa gttttttaga tactgaaact ggtaattatg tttatgtaag cataaggagt
    24721 tcagattgaa ggattctaca gggcaaatga ggctggcaac caaggatttg gcggaagcca
    24781 ttaaacgagg agaagtacgt agttctgctt ttacaacaaa gcaactaaag gcaatcgaaa
    24841 aaggagaatc taaaataccg gattacactt ggcatcatca tcaagacact ggaaggatgc
    24901 aattgattaa tgaagacttg catcatgata ccggccatat cggttggaga gcgatgagta
    24961 aaggaaagta actatgtgga aaatcataaa agaggatagt gatgatttag gatttgcaat
    25021 taaatgctta ttctctcaat ctattgattt aaatgaattc aaattatgga ttgaacaagt
    25081 aatacgagat atgcccatcg aggacatccc tttttatatt tttgacttgg cggatttcga
    25141 tggggtaatt gccgatattg acaatattgt aggttttgtt tcgagttaca gcctatcaaa
    25201 atcgaaaaaa aaatgccttg accggcattg ccttcttaag ggggatagat gtctatgacc
    25261 ctcccatttc aaaagaaaaa gcattaaaag ccttagagaa acatcctgaa atttatcaga
    25321 aatttcagca tttctttccg tttgtagagc tgcccccgct ttaaacagtc aaaatgccgt
    25381 ctgaaacaat attcggcttt cagacggcat ttttgataca aagcaggtaa ccaaaagagc
    25441 gtttgacggc aaaggaagat aattatgtgg aaaattacga aagagcaagg tcaagattta
    25501 agttttgcaa catattgctt attatcggca gcaattaatt tgcaagaatt taaattatgg
    25561 ttggagaagg ttgtcttaga tatgcctatt gataatatac atttttatat ttttgattta
    25621 attgatctta aagaaggagt aggagatatt tataatattt tggattttgt tccgaattca
    25681 gacttatcta aagaccaaga tgacgcatta acaggcattg ccttcttaag gggtatagat
    25741 gtctatgacc ctcccgtttc aaaagaaaaa gcattaaaag ccttaaagaa acatcctgaa
    25801 atttatcaga ggtttcagca tttctttccg tttgtagaac tgcccccgct ttaaacagtc
    25861 aaaatgccgt ctgaaagacc gttccgccgt tcagacggca ttttcgcccc ttttgtttac
    25921 aaacccttaa aatcccttta cactcaaaat ccgttcaaca tcaaacaaac cccgc
//
LOCUS       DAXHSF010000011        24775 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.011, whole genome shotgun sequence.
ACCESSION   DAXHSF010000011 DAXHSF010000000
VERSION     DAXHSF010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24775
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.011"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            466..1110
                     /gene="rplC"
                     /locus_tag="ACFM8S_000360"
     CDS             466..1110
                     /gene="rplC"
                     /locus_tag="ACFM8S_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFC6313484.1"
                     /translation="MTLGLVGRKVGMTRVFDEQGVSVPVTVLDMSANRVTQVKSKDTD
                     GYTAVQVTFGQKKANRVNKTEAGHFAKAGVEAGRGLIEFALTEEKLAELKAGDEITVS
                     MFEVGQLVDVTGTSKGKGFSGTIKRHNFGAQRTSHGNSRSHRVPGSIGMAQDPGRVFP
                     GKRMAGQYGNTKATVQKLEVVRVDAERQLLLVKGAVPGAVNSDVVVRPSVKVGA"
     gene            1110..1730
                     /gene="rplD"
                     /locus_tag="ACFM8S_000361"
     CDS             1110..1730
                     /gene="rplD"
                     /locus_tag="ACFM8S_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFC6313485.1"
                     /translation="MELKVIDAKGQVSGSLSVSDALFAREYNEALVHQLVNAYLANAR
                     SGNRAQKTRAEVKHSTKKPWRQKGTGRARSGMTSSPLWRKGGRAFPNKPDENFTQKVN
                     RKMYRAGMATILSQLTRDERLFAIEALTAETPKTKVFAEQVKNLGLEQVLFVTKQLDE
                     NVYLASRNLPNVLVLEAQQVDPYSLLRYKKVIITKDAVAQLEEQWV"
     gene            1727..2041
                     /gene="rplW"
                     /locus_tag="ACFM8S_000362"
     CDS             1727..2041
                     /gene="rplW"
                     /locus_tag="ACFM8S_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFC6313486.1"
                     /translation="MNQQRLTQVILAPIVSEKSNVLAEKRNQMTFKVLANATKPEIKA
                     AVELLFGVQVASVTTVTTKGKTKRFGRTLGRRSDVKKAYVSLAAGQELDLEAAAAAAD
                     KE"
     gene            2047..2880
                     /gene="rplB"
                     /locus_tag="ACFM8S_000363"
     CDS             2047..2880
                     /gene="rplB"
                     /locus_tag="ACFM8S_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFC6313487.1"
                     /translation="MAIVKMKPTSAGRRGMVRVVTEGLHKGAPYAPLLEKKNSTAGRN
                     NNGHITTRHKGGGHKHHYRVVDFKRNKDGIPAKVERIEYDPNRTAFIALLCYADGERR
                     YIIAPRGIQAGAVLVSGAEAAIKVGNTLPIRNIPVGTTIHCIEMKPGKGAQIARSAGA
                     SAVLLAKEGAYAQVRLRSGEVRKINVNCRATIGEVGNEEQSLKKIGKAGANRWRGIRP
                     TVRGVVMNPVDHPHGGGEGRTGEAREPVSPWGTPAKGYRTRNNKRTDNMIVRRRYSNK
                     G"
     gene            2887..3165
                     /gene="rpsS"
                     /locus_tag="ACFM8S_000364"
     CDS             2887..3165
                     /gene="rpsS"
                     /locus_tag="ACFM8S_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFC6313488.1"
                     /translation="MARSLKKGPYVDLHLLKKVDAARASNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGRTHVPVFISDNMVGHKLGEFSLTRTFKGHLADKKAKKK"
     gene            3174..3503
                     /gene="rplV"
                     /locus_tag="ACFM8S_000365"
     CDS             3174..3503
                     /gene="rplV"
                     /locus_tag="ACFM8S_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFC6313489.1"
                     /translation="MRVNAQHKNARISAQKARLVADLIRGKDVAQALNILAFSPKKGA
                     ELIKKVLESAIANAEHNNGADIDELKVVTIFVDKGPSLKRFQARAKGRGNRIEKQTCH
                     INVTVGN"
     gene            3513..4205
                     /gene="rpsC"
                     /locus_tag="ACFM8S_000366"
     CDS             3513..4205
                     /gene="rpsC"
                     /locus_tag="ACFM8S_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFC6313490.1"
                     /translation="MGQKINPTGFRLAVTKDWASKWFAKSTDFSTVLKQDIDVRNYLR
                     QKLANASVGRVVIERPAKSARITIHSARPGVVIGKKGEDIEVLKRDLQVLMGVPVHVN
                     IEEIRRPELDAQIIADGIAQQLEKRVQFRRAMKRAMQNAMRSGAKGIKIMTSGRLNGA
                     DIARSEWYREGRVPLHTLRANVDYATSEAHTTYGVLGLKVWVYTEGNIKSSKPEHESK
                     QRKAGRRNAAAN"
     gene            4189..4605
                     /gene="rplP"
                     /locus_tag="ACFM8S_000367"
     CDS             4189..4605
                     /gene="rplP"
                     /locus_tag="ACFM8S_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFC6313491.1"
                     /translation="MLQPTRLKYRKQQKGRNTGIATRGNKVSFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRVFPDKPITEKPIQVRMGGGKGNVEYYIAEIKPGKV
                     LYEMDGVPEELAREAFELAAAKLPIPTTFVVRQVGQ"
     gene            4605..4796
                     /gene="rpmC"
                     /locus_tag="ACFM8S_000368"
     CDS             4605..4796
                     /gene="rpmC"
                     /locus_tag="ACFM8S_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFC6313492.1"
                     /translation="MKANELKDKSVEQLNADLLDLLKAQFGLRMQNATGQLGKPSELK
                     RVRRDIARIKTVLTEKGAK"
     gene            4796..5059
                     /gene="rpsQ"
                     /locus_tag="ACFM8S_000369"
     CDS             4796..5059
                     /gene="rpsQ"
                     /locus_tag="ACFM8S_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFC6313493.1"
                     /translation="MSETKNVRTLQGKVVSDKMDKTVTVLVERKVKHPLYGKIIRLST
                     KIHAHDENNQYGIGDVVVISESRPLSKTKSWVVSELVEKARSI"
     gene            5281..5649
                     /gene="rplN"
                     /locus_tag="ACFM8S_000370"
     CDS             5281..5649
                     /gene="rplN"
                     /locus_tag="ACFM8S_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFC6313494.1"
                     /translation="MIQMQTILDVADNSGARRVMCIKVLGGSKRRYASVGDIIKVAVK
                     DAAPRGRVKKGDVYNAVVVRTAKGVRRPDGALIKFDNNAAVLLNNKLEPLGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            5661..5984
                     /gene="rplX"
                     /locus_tag="ACFM8S_000371"
     CDS             5661..5984
                     /gene="rplX"
                     /locus_tag="ACFM8S_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFC6313495.1"
                     /translation="MNKIIKGDRVVVIAGKDKGKQGQVVRVLGDKVVVEGVNVVKRHQ
                     KPNPMRGIEGGIITKEMPLDISNIAILNPETNKADRVGIKLIENEGKVKRVRFFKSNG
                     SIIGA"
     gene            5994..6533
                     /gene="rplE"
                     /locus_tag="ACFM8S_000372"
     CDS             5994..6533
                     /gene="rplE"
                     /locus_tag="ACFM8S_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFC6313496.1"
                     /translation="MARLREFYKETVVPELVKQFGYKSVMEVPRIEKITLNMGVGEAI
                     ADKKVMEHAVSDLEKIAGQKPVVTVARKSIAGFKIRDNYPVGCKVTLRRDQMFEFLDR
                     LITIALPRVRDFRGVSGKSFDGRGNYNMGVREQIIFPEIEYDKIDALRGLNITITTTA
                     KTDEEAKALLSLFKFPFKG"
     gene            6536..6841
                     /gene="rpsN"
                     /locus_tag="ACFM8S_000373"
     CDS             6536..6841
                     /gene="rpsN"
                     /locus_tag="ACFM8S_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFC6313497.1"
                     /translation="MAKKALINRDLKRQALAKKYAAKRAAIKAVINDSNATEEERFEA
                     RLRFQSIPRNAAPVRQRRRCALTGRPRGTFRKFGLGRIKIREIAMRGEIPGVVKASW"
     gene            6857..7249
                     /gene="rpsH"
                     /locus_tag="ACFM8S_000374"
     CDS             6857..7249
                     /gene="rpsH"
                     /locus_tag="ACFM8S_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFC6313498.1"
                     /translation="MSMHDPISDMLTRIRNAQRANKAAVAMPSSKLKCAIAKVLKEEG
                     YIEDFAVSSDVKSILEIQLKYYAGRPVIEQIKRVSRPGLRIYKASSEIPSVMNGLGIA
                     IVSTSKGVMTDRKARSQGVGGELLCIVA"
     gene            7263..7796
                     /gene="rplF"
                     /locus_tag="ACFM8S_000375"
     CDS             7263..7796
                     /gene="rplF"
                     /locus_tag="ACFM8S_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFC6313499.1"
                     /translation="MSRVAKNPVTVPAGVEVKFGTEALVIKGKNGELSFPLHSDVAIE
                     FNDGKLTFVANNSSKQANAMSGTARALVSNMVKGVSEGFEKRLQLIGVGYRAQAQGKI
                     LNLSLGFSHPIVYEMPEGVSVQTPSQTEIVLTGSDKQVVGQVAAEIRAFRAPEPYKGK
                     GVRYVGEVVVMKEAKKK"
     gene            7810..8163
                     /gene="rplR"
                     /locus_tag="ACFM8S_000376"
     CDS             7810..8163
                     /gene="rplR"
                     /locus_tag="ACFM8S_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFC6313500.1"
                     /translation="MDKHTTRLRRARKTRARIADLKMVRLCVFRSNNHIYAQVISAEG
                     DKVLAQASTLEAEVRGSLKSGSNVEAAAIVGKRIAEKAKAAGVEKVAFDRSGFQYHGR
                     VKALAEAARENGLSF"
     gene            8182..8700
                     /gene="rpsE"
                     /locus_tag="ACFM8S_000377"
     CDS             8182..8700
                     /gene="rpsE"
                     /locus_tag="ACFM8S_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFC6313501.1"
                     /translation="MAKHEIEERGDGLIEKMVAVNRVTKVVKGGRIMAFSALTVVGDG
                     DGRIGMGKGKSKEVPVAVQKAMDQARRSMIKVPLKNGTIHHEVIGRHGATKVFMQPAK
                     EGSGVKAGGPMRLVFDAMGIHNISAKVHGSTNPYNIVRATLDGLSKLHTPADIAAKRG
                     LTVEDILGVNHG"
     gene            8693..8878
                     /gene="rpmD"
                     /locus_tag="ACFM8S_000378"
     CDS             8693..8878
                     /gene="rpmD"
                     /locus_tag="ACFM8S_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFC6313502.1"
                     /translation="MAEQKKIRVTLVKSLIGTIESHRACARGLGLRRREHTVEVLDTP
                     ENRGMINKISYLLKVES"
     gene            8880..9314
                     /gene="rplO"
                     /locus_tag="ACFM8S_000379"
     CDS             8880..9314
                     /gene="rplO"
                     /locus_tag="ACFM8S_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFC6313503.1"
                     /translation="MFLNTIQPAVGATHAGRRVGRGIGSGLGKTGGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFKSLTASANAQLRLSELESIAVNEIDILVLKQAGLIA
                     STVSNVKVIASGEISKAVALKGIKVTKGARAAIEAVGGKIEM"
     gene            9326..10636
                     /gene="secY"
                     /locus_tag="ACFM8S_000380"
     CDS             9326..10636
                     /gene="secY"
                     /locus_tag="ACFM8S_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFC6313504.1"
                     /translation="MANQQTSSGSSKFGDLKKRLLFLFGALIVFRIGAHIPVPGVDAV
                     ALAKLYESAGNGILGILNMFSGGSLERFSIFAIGIMPYISASIIVQLASEILPSLKAL
                     KKEGEAGRKVITKYTRYGTVLLAILQSLGVASFVFQQGIVVTSSFEFHVSTVVSLVTG
                     TMFLMWLGEQITERGIGNGISLIITAGIASGIPSGIAKLVTLTNQGSMSMLTALFIVF
                     GALLLIYLVVYFESAQRKIPIHYAKRQFNGRAGSQNTHMPFKLNMAGVIPPIFASSII
                     LFPSTLLGWFGSADTNSVLHKIAGLLQHGQLLYMALFAATVIFFCYFYTALVFSPKEM
                     AENLKKSGAFVPGIRPGEQTSRYLEKVVLRLTLFGALYITTICLIPEFLTTVLNVPFY
                     LGGTSLLILVVVTMDFSTQINSYRLTQQYDKLMTRSEMKSFSRK"
     gene            10641..10859
                     /gene="infA"
                     /locus_tag="ACFM8S_000381"
     CDS             10641..10859
                     /gene="infA"
                     /locus_tag="ACFM8S_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFC6313505.1"
                     /translation="MAKEDTIQMQGEILETLPNATFKVKLENDHIVLGHISGKMRMHY
                     IRISPGDKVTVELTPYDLTRARIVFRAR"
     gene            10880..10993
                     /gene="rpmJ"
                     /locus_tag="ACFM8S_000382"
     CDS             10880..10993
                     /gene="rpmJ"
                     /locus_tag="ACFM8S_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFC6313506.1"
                     /translation="MRVQPSVKKICRNCKIIRRNRVVRVICTDLRHKQRQG"
     gene            11059..11421
                     /gene="rpsM"
                     /locus_tag="ACFM8S_000383"
     CDS             11059..11421
                     /gene="rpsM"
                     /locus_tag="ACFM8S_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFC6313507.1"
                     /translation="MARIAGVNIPNNAHIVIGLQAIYGIGATRAKLICEAANIAPDTK
                     AKDLDETQLDALRDQVAKYEVEGDLRREVTMSIKRLMDMGCYRGFRHRRGLPCRGQRT
                     RTNARTRKGPRKAIAGKK"
     gene            11441..11836
                     /gene="rpsK"
                     /locus_tag="ACFM8S_000384"
     CDS             11441..11836
                     /gene="rpsK"
                     /locus_tag="ACFM8S_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFC6313508.1"
                     /translation="MAKANTASRVRKKVRKTVSEGIVHVHASFNNTIITITDRQGNAL
                     SWATSGGAGFKGSRKSTPFAAQVAAEAAGKVAQEYGVKNLEVRIKGPGPGRESSVRAL
                     NALGFKITSITDVTPLPHNGCRPPKKRRI"
     gene            11856..12476
                     /gene="rpsD"
                     /locus_tag="ACFM8S_000385"
     CDS             11856..12476
                     /gene="rpsD"
                     /locus_tag="ACFM8S_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFC6313509.1"
                     /translation="MARYIGPKCKLARREGTDLFLKSARRSLDSKCKIDSAPGQHGAK
                     KPRLSDYGLQLREKQKIRRIYGVLERQFRRYFAEADRRKGSTGELLLQLLESRLDNVV
                     YRMGFGSTRAEARQLVSHKAIVVNGQVVNIPSFQVKAGDVVSVREKAKKQVRIQEALG
                     LATQIGLPGWVSVDADKLEGVFKNMPDRSELTGDINEQLVVEFYSK"
     gene            12502..13488
                     /gene="rpoA"
                     /locus_tag="ACFM8S_000386"
     CDS             12502..13488
                     /gene="rpoA"
                     /locus_tag="ACFM8S_000386"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFC6313510.1"
                     /translation="MQNSTTEFLKPRQIDVNTFSATRAKVSMQPFERGFGHTLGNALR
                     RILLSSMNGFAPTEVAIAGVLHEYSTVDGIQEDVVDILLNIKGIVFKLHGRSQVQLVL
                     KKSGSGVVSAGDIELPHDVEILNPGHVICHLADNGQIEMEIKVEQGRGYQSVSGRQVV
                     RDENRQIGAIQLDASFSPISRVSFEVEPARVEQRTDLDKLVLDIETDGSIDPEEAVRS
                     AARILIDQMSIFADLQGTPVEEVEEKAPPIDPVLLRPVDDLELTVRSANCLKAEDIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGSKLEAWPPVGLEKP"
     gene            13512..13880
                     /gene="rplQ"
                     /locus_tag="ACFM8S_000387"
     CDS             13512..13880
                     /gene="rplQ"
                     /locus_tag="ACFM8S_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFC6313511.1"
                     /translation="MRHRNGNRKLNRTSSHRAAMLRNMANSLLTHEAIVTTLPKAKEL
                     RRVVEPLITLGKKPSLANRRLAFDRTRDRDVVVKLFGDLGPRFTARNGGYVRVLKYGF
                     RKGDNAPLALVELVDKPAAE"
     gene            14027..14740
                     /gene="minC"
                     /locus_tag="ACFM8S_000388"
     CDS             14027..14740
                     /gene="minC"
                     /locus_tag="ACFM8S_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFC6313512.1"
                     /translation="MMVYIMNAFDIKSTKMDVLSISLHTSDLFDLEDVLVKLGKKFQE
                     SGVVPFVLDVQEFDYPESLDLAALVSLFSRHGMQILGLKHSNERWAAAAMKYHLLFCL
                     SHSENVKELGQVEVQNTEDGQKARKTVLITSPVRTGQQVYAEDGDLIVTGAVSQGAEL
                     IADGNMHIYAPMRGRALAGAKGDTSARIFIHSMQAELVSVAGIYRNFEQDLPDHLHKQ
                     PVQILLQDNRLVISAIGSE"
     gene            14769..15584
                     /gene="minD"
                     /locus_tag="ACFM8S_000389"
     CDS             14769..15584
                     /gene="minD"
                     /locus_tag="ACFM8S_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFC6313513.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASIATGLALRGYKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEATLNQALIKDKNCENLFILPASQTRDKDALTREGV
                     EKVMQELSGKKMGFEYIICDSPAGIEQGALMALYFADEAIVTTNPEVSSVRDSDRILG
                     ILQSKSHKAEQGGSVKEHLLITRYSPERVAKGEMLSVQDICDILHIPLLGVIPESQNV
                     LQASNSGEPVIHQDSVAASEAYKDVIARLLGENREMRFLEAEKKSFFKRLFGG"
     gene            15588..15851
                     /gene="minE"
                     /locus_tag="ACFM8S_000390"
     CDS             15588..15851
                     /gene="minE"
                     /locus_tag="ACFM8S_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFC6313514.1"
                     /translation="MSLIEFLFGRKQKTATVARDRLQIIIAQERAQEGQAPDYLPTLR
                     KELMEVLSKYVNVSLDNIRISQEKQDGMDVLELNITLPEQKKV"
     gene            15855..16775
                     /gene="oxyR"
                     /locus_tag="ACFM8S_000391"
     CDS             15855..16775
                     /gene="oxyR"
                     /locus_tag="ACFM8S_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator OxyR"
                     /protein_id="HFC6313515.1"
                     /translation="MTLTELRYIVAVAQERHFGRAARRCFVSQPTLSIAIKKLEEELA
                     VSLFDRSSNDIITTEAGERIVAQARKVLEEAELIRHLANEEQNELEGAFKLGLIFTVA
                     PYLLPKLIVSLRRTAPKMPLMLEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPL
                     YDEPFFVIVPKGHSFEELDAVSPRMLGEEQVLLLTEGNCMRDQVLSSCSELAAKQRIQ
                     GLTNTLQGSSINTIRHMVASGLAISVLPATALTENDHMLFSIIPFEGTPPSRRVVLAY
                     RRNFVRPKALSAMKAAIMQSQLHGVSFIRD"
     gene            complement(16772..17005)
                     /locus_tag="ACFM8S_000392"
     CDS             complement(16772..17005)
                     /locus_tag="ACFM8S_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313516.1"
                     /translation="MAIVRQGEATPYWFLLIHYTWHCIGTDENIHSDAKVEYLQDGSF
                     RGDAILKIDDDGNILAYRVVGSGKWRFENNACA"
     gene            17349..17738
                     /locus_tag="ACFM8S_000393"
     CDS             17349..17738
                     /locus_tag="ACFM8S_000393"
                     /inference="COORDINATES: protein motif:HMM:NF017109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HFC6313517.1"
                     /translation="MRYTIKTFQTLADETFPSKNFKILSFNGVARPITIVCPTHGVQT
                     VSAGQAFIRSKHGCPACGLVLSTSVLASRGRSVSILDTATGETLSFPSVQAAAKALNT
                     SYGSIRTKLDGRSSPDNLVCNRYKVMP"
     gene            complement(18108..20945)
                     /locus_tag="ACFM8S_000394"
     CDS             complement(18108..20945)
                     /locus_tag="ACFM8S_000394"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFC6313518.1"
                     /translation="MLDKYNPAEIESKHYQNWEEQGYFQPDMDLTKPSFSIQLPPPNV
                     TGTLHMGHAFNQTIMDGLTRYYRMKGCNTAWIPGTDHAGIATQIVVERQLAAQNVSRH
                     DLGREKFLEKVWEWKEVSGGTITQQMRRVGCSADWTREYFTMDDVRAETVTEVFVRLF
                     EQGLIYRGKRLVNWDPVLGTAVSDLEVESVEEQGSMWHIRYPLADNPAEAVIVATTRP
                     ETLLGDVAVAVNPEDERYTHLIGKELILPLTGRTIPVIADEYVEKDFGTGCVKITPAH
                     DFNDYEVGKRHDTRLINVFDLEAKVLANAEVFNFKGEAQPGFALPEKYAGLDRFAARK
                     QMVADLQEQGLLVEIKAHTLMTPKGDRTGSVIEPMLTSQWFVAMSATPNGGEPDSEFK
                     GLSLADKAKKAVDSGAVRFIPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDEAG
                     NVYVARNQAEAEKQAGKTGLTREEDVLDTWFSSALVPFSTLGWPSETDELKAFLPSNV
                     LVTGYEIIFFWVARMIMMTTHFTGKVPFKDVYIHGIVRDHEGKKMSKSEGNVIDPVDL
                     IDGIDLDKLLVKRTTGLRKPETAPKVEEATKKLFPEGIPSMGADALRFTMASYASLGR
                     SVNFDFKRAEGYRNFCNKIWNATNFVLMNTENQDCGYGATATEPRGYSFPDMWIVGRL
                     NQTIEQVTQAYETYRFDLAAETLYSFVWNDYCDWYLELAKVQLQTGCASRQRATRHTL
                     LRVLEAALRLLHPIIPFITEELWQTVAPMCDAKTADSIMLARFPETDGGEIVQTAFGQ
                     MTVLQDLIGAVRNLRGEMGIQPNVKAPLFVESADDLADYLKYLPMMTRLTEARQVAAL
                     PESEDAPVAVCNGARLMLKVEIDKAAETARLSKEAEKLQKALDKLNAKLSKPGYTEKA
                     PAHLVEKDKADLAELEDKMAKVQTQLSKLKD"
     gene            complement(21042..21851)
                     /gene="zupT"
                     /locus_tag="ACFM8S_000395"
     CDS             complement(21042..21851)
                     /gene="zupT"
                     /locus_tag="ACFM8S_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFC6313519.1"
                     /translation="MPDFSMSNLAVAFSITLAAGLFTVLGSGLVMFSKTPNPRVLSFG
                     LAFAGGAMVYVSLTEIFSKSSEAFAEIYDKDHAFAAATMAFLAGMGGIALIDRLVPNP
                     HETLDAQDPSFQESKRRHIARVGMMAAFAITAHNFPEGLATFFATLENPAVGMPLALA
                     IAIHNIPEGISIAAPVYFATRSRKKTVWACLLSGLAEPLGAALGYLVLQPFLSPAVFG
                     SVFGVIAGVMVFLALDELLPAAKRYSDGHETVYGLTMGMAVIAVSLVLFHF"
     gene            complement(22032..23288)
                     /locus_tag="ACFM8S_000396"
     CDS             complement(22032..23288)
                     /locus_tag="ACFM8S_000396"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFC6313520.1"
                     /translation="MKVLVLGAGVAGVSSAWYLAEAGHEVTVIDRAEGVAMETSFANA
                     GQLSYGYTTPWAAPGIPTKALKWLFKSHPPLLFRPDGSLYQIEWLWQMLQNCTAAHYQ
                     TNKERMVRISEYSREMFRRFEAQTGMNFEERKKGTLQIFRQTKEVEAAEQDIAVLERY
                     GVPYRRLKPEECAEFEPALARVTAKIASGLHLPADATGDCRLFTENLYKLCQEKGVRF
                     HFNQTISRIDHNGLRIKTVETETGRFEADAVVCALGCFSRTVLAQLDLNLPIYPVKGY
                     SLTLPVTNSDGAPVSTVLDESYKVAITRFDNRIRVGGMAELSGYEIKLPEKRRETLAL
                     VVNDLFPEGGDLSQASFWSGLRPMTPDSTPLIGRTRFDNLFLNTGHGTLGWTMSLGSA
                     KLTADIVSGKDTEIRSDDLSLSRYQA"
     gene            complement(23367..24758)
                     /locus_tag="ACFM8S_000397"
     CDS             complement(23367..24758)
                     /locus_tag="ACFM8S_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC6313521.1"
                     /translation="MQVFLDNPKAFFETVSGWVWGPLMLMLLVGTGILLTVLLKGLQF
                     TMLGYALKQAFMPPKKHKSGEGHEGDISHFAALMTALSATIGTGNIAGVATAVVTGGP
                     GAVFWMWMTAIFGMATKYGEGVLAVKYRVNNSKGEMSGGPMYYIEKGLGKNWKWMAVA
                     FALFGTFASFGIGSSVQSNSVAQAVQTSFGIEPAYTGITLTVLTAVVVLGGIKGIAKA
                     ASFIVPAMAVFYVLGGLSIIAINSDALMPAVKLIFSDAFSAQAVAGGAIGTVIRYGVA
                     RGVFSNEAGMGSAPIAAAAAKTDHPVRQALVSMTGTFLDTIVVCSITGIVLVMGLLGA
                     GGEFVKPEVSGAALTTVTFQKMLPGIGGWIVTIGLIFFAYSTILGWCYYGEKCAVYVF
                     GEKFAGLYRVGYVSSVMLGTVLSLDLVWLASDTFNGLMALPNLIALLLMAKVIVNETR
                     DFKQKITNGELPH"
BASE COUNT         6407 a         5256 c         6295 g         6817 t
ORIGIN      
        1 taagtgcgtc cgctgccgcc taaaaagcag cccggatgcc tgattatcgg gtatccgggc
       61 aggattaagg gggtatttgg ggcgaaaaca gctgaaaaca gccgaaaacc tgtgtttggg
      121 tttcggctgt cgggagggaa aggaattttg caaaggtccc agcatacgaa aactaccatc
      181 tcaaagaaat aatggatgca gtcatcaacc atattccggt ttgtcggaga aagatgcata
      241 cgctgtgatg accggatacc gaccctttaa aagagtccgg ccctatgccg tctgaagatt
      301 caaaacgctt cagacggcat attgaagata tttctgatat ttctgttgat atttctttga
      361 cttgtcagat ataatgccga gcttggtaca tttgtgccaa gtttaacttt gtctgaaaga
      421 caggccaatc gtagcctgtc cctttacttt aaaaggaaaa taatcatgac tttaggtctg
      481 gttggacgca aagttggtat gacccgcgtg ttcgacgaac agggtgtttc tgttccggta
      541 accgttttgg atatgtctgc caaccgcgtt acacaagtaa aatccaaaga tactgacggc
      601 tatactgccg ttcaagttac ctttggtcag aaaaaagcca atcgtgtcaa caaaaccgaa
      661 gccgggcact ttgcaaaagc aggtgttgaa gccggtcgtg gtttgatcga gtttgctttg
      721 actgaagaaa aactggctga attgaaagcc ggtgacgaaa tcaccgtttc tatgtttgaa
      781 gtcggccagc tggtcgatgt aaccggtacc tctaaaggta aaggtttctc cggcacgatc
      841 aaacgtcata acttcggtgc ccaacgcact tcccacggta actcccgttc tcaccgcgtt
      901 ccaggctcta tcggtatggc gcaagacccg ggtcgcgtgt tccccggtaa gcgcatggcc
      961 ggccaatacg gcaacaccaa agcaactgtt caaaaattgg aagttgtccg tgttgatgca
     1021 gaacgccaac tgctgttggt taagggtgct gttccgggtg cggtcaacag cgatgttgta
     1081 gttcgtccta gcgtgaaagt aggtgcgtaa tggaattgaa agtaattgac gctaaaggac
     1141 aagtttcagg cagcctgtct gtttctgatg ctttgttcgc ccgcgaatac aatgaagcgt
     1201 tggttcatca gctggtaaat gcctacttgg caaacgcccg ctccggtaac cgcgctcaaa
     1261 aaacccgtgc cgaagtaaaa cactcaacca aaaaaccatg gcgtcaaaaa ggtaccggcc
     1321 gtgcccgttc cggtatgact tcttctccgc tgtggcgtaa aggtggtcgc gcgttcccga
     1381 acaaacccga cgaaaacttc actcaaaaag taaaccgcaa aatgtaccgt gccggtatgg
     1441 cgactattct gtctcaattg actcgtgacg agcgtttgtt tgcgattgag gcgttgactg
     1501 ccgaaactcc taaaaccaaa gtttttgctg aacaagtgaa aaatctgggt ctggagcaag
     1561 tgttgtttgt aaccaaacag ctcgacgaga atgtttactt ggcttcacgc aacttgccaa
     1621 acgtgttggt tttggaagct caacaagttg atccttacag cttgctgcgt tacaaaaaag
     1681 taatcatcac taaagatgca gttgcacaat tagaggagca atgggtatga atcaacaacg
     1741 tttgactcaa gtgattttgg cacctatcgt ttctgaaaaa agcaacgtat tggctgaaaa
     1801 acgcaaccaa atgacgttta aagttttggc aaatgcaacc aaacctgaaa tcaaagcggc
     1861 tgttgagctg ctgtttggtg ttcaagttgc ttctgtaact accgttacaa ctaaaggcaa
     1921 aactaagcgt tttggtcgta ctttgggccg ccgcagcgat gttaaaaaag cttatgtaag
     1981 cttggctgcc ggtcaagagt tggatttgga agccgctgct gcagctgcag ataaggaata
     2041 aacaaaatgg caatcgttaa aatgaagccg acctctgcag gccgtcgcgg catggttcgc
     2101 gtggtaacag aaggtttgca caaaggtgca ccttatgcgc ccttgcttga aaagaaaaat
     2161 tctactgccg gccgtaacaa taatggtcat atcaccaccc gtcacaaagg cggcggtcat
     2221 aaacaccatt accgtgttgt agactttaaa cgtaacaaag acggcattcc tgccaaagta
     2281 gagcgtatcg aatacgatcc taaccgtact gccttcatcg cactgttgtg ctatgcagac
     2341 ggcgagcgtc gttacatcat tgctcctcgc ggtattcaag ccggtgctgt attggtttcc
     2401 ggtgctgaag ctgccatcaa agtaggcaac accctgccga ttcgcaacat tcccgttggt
     2461 acgactatcc actgtatcga aatgaaaccc ggtaaaggtg ctcaaatcgc acgttctgcc
     2521 ggtgcttctg cggtattgct ggctaaagaa ggcgcgtacg ctcaagtccg tctgcgctct
     2581 ggcgaagtcc gtaaaatcaa cgtaaattgc cgtgcgacca ttggtgaagt tggtaacgaa
     2641 gagcaaagcc tgaaaaaaat cggtaaagcc ggtgccaacc gttggcgcgg tattcgtccg
     2701 accgtacgcg gtgttgtcat gaatcctgtc gatcacccgc atggtggtgg tgaaggccgt
     2761 acgggcgagg cccgcgaacc ggtcagccca tggggtactc ctgctaaagg ctaccgcact
     2821 cgtaataaca aacgcacgga taacatgatt gttcgtcgcc gttactcaaa taaaggttaa
     2881 tttagtatgg ctcgttcatt gaaaaaaggc ccatatgtag acctgcattt gctgaaaaaa
     2941 gtagatgctg ctcgcgcaag caacgacaaa cgcccgatta aaacctggtc tcgtcgttct
     3001 accattctgc ctgattttat cggtctgacc attgctgtgc acaacggccg cacccatgtg
     3061 cctgtgttta tcagcgacaa tatggttggt cataaattag gcgaattctc attgacccgt
     3121 acctttaaag gccacttggc cgataaaaag gctaaaaaga aataaggtga atcatgagag
     3181 taaatgcaca acataaaaat gcccgtatct ctgctcaaaa ggctcgtttg gtagctgatt
     3241 tgattcgtgg taaagacgtt gcccaagctt tgaatatttt ggctttcagt cctaaaaaag
     3301 gtgccgagct gattaaaaaa gtattggagt cagctattgc taatgccgag cacaataacg
     3361 gtgcggacat tgatgaactg aaagtggtaa ctatctttgt tgacaaaggc ccaagcttga
     3421 aacgttttca agctcgcgcc aaaggtcgcg gtaaccgcat cgaaaaacaa acttgtcata
     3481 tcaatgtgac agtgggtaac taaggaaaag ctatgggaca aaagattaac cctacaggct
     3541 ttcgcctggc ggtaactaaa gactgggctt caaaatggtt tgctaaaagc accgactttt
     3601 ctactgtttt gaagcaggat atcgatgttc gcaattattt gcgtcaaaaa ttggccaatg
     3661 cttcggttgg tcgagtggtt attgaacgcc ctgcaaaatc tgcacgcatt accattcact
     3721 ccgctcgtcc gggtgtggtt atcggtaaaa aaggtgagga tatcgaggtt ttgaaacgtg
     3781 acttgcaagt cttgatgggt gtacctgttc atgtaaatat tgaagagatt cgccgtcctg
     3841 agttggatgc tcaaattatt gctgacggta ttgcccagca gttggaaaag cgcgttcaat
     3901 tccgtcgtgc tatgaaacga gcaatgcaaa atgcaatgcg ttctggtgct aaaggcatta
     3961 agattatgac ttcaggccgt ctgaatggtg cggatattgc ccgtagcgaa tggtatcgtg
     4021 aaggtcgcgt gccactgcat actttacgtg caaatgtaga ttatgcaacc agcgaagcgc
     4081 acaccacata tggtgtattg ggtctgaaag tttgggttta tacggaaggc aatattaaat
     4141 cttccaaacc tgaacatgag agtaaacaaa gaaaggcagg tagacgtaat gctgcagcca
     4201 actagactga aataccgtaa gcaacaaaag ggtcgcaata ctggcatcgc tactcgcggt
     4261 aataaggtaa gtttcggtga gttcggcttg aaagccgtag gtcgtggtcg tttgactgcc
     4321 cgtcaaatcg aagctgctcg tcgtgcaatg acccgtcata tcaaacgtgg tggtcgtatt
     4381 tggattcgtg tattccctga taaaccgatt actgaaaagc ctattcaagt tcgtatgggt
     4441 ggcggtaaag gtaacgtgga atattacatt gccgaaatta aaccaggtaa agtgttgtat
     4501 gaaatggatg gcgttccaga ggaactggct cgtgaagcat tcgagttggc tgctgccaaa
     4561 ttgcctattc ctacaacctt tgtagtaaga caggtgggtc aataatgaaa gcaaatgaat
     4621 tgaaagacaa atccgttgag cagttgaatg cagatttgtt ggacttgttg aaagctcagt
     4681 ttggcttacg tatgcaaaac gctaccggtc aattaggcaa accaagtgaa ttgaaacgtg
     4741 tacgtcgcga tattgctcgt attaaaaccg ttttaactga aaaaggtgct aagtaatgag
     4801 cgaaactaaa aatgttcgta ctttgcaagg caaagtagta agcgacaaaa tggataaaac
     4861 cgtaacagta ttggttgagc gtaaagtaaa acatccgctg tatggtaaga ttattcggtt
     4921 atctactaaa atccatgccc atgatgaaaa taatcaatat ggaattggtg atgtggttgt
     4981 tatatcggaa tcccgtccat tgtcaaaaac taaatcttgg gttgtcagtg agctggttga
     5041 gaaagcacgt tctatttaag aattaaagca acgtgcttgg aatgggaaac gaagtattgc
     5101 agcaaattta atttgcgtgt aaacttcgtt tcctgtcttt cagtttcttc tggaagtttc
     5161 ttccctttcg gggtccaaga ctggtttact tgaaccgcaa ggtttcattt aataagcagc
     5221 ggctttgctg taagttatct gaaagtggta aattaagttg gttaatttaa aggtaataac
     5281 atgattcaaa tgcagaccat cttagatgtg gctgataact ctggtgcgcg tcgcgtaatg
     5341 tgtatcaagg tattgggcgg atctaagcgt cgctacgctt ctgttggcga tattattaaa
     5401 gtggcagtta aagatgcggc tccgcgtggc cgtgtcaaaa aaggcgatgt atataatgcg
     5461 gtagttgttc gtactgctaa gggtgtacgt cgtcctgatg gtgcgttaat taaattcgat
     5521 aacaatgccg ccgtgttact gaataataaa cttgaacctt tgggtactcg tatctttggt
     5581 ccggtaaccc gtgaattgcg tactgagcga tttatgaaaa tcgtttcatt ggcacctgaa
     5641 gtattataag gaatggcacg atgaataaaa tcattaaagg cgatagggtt gtagtaattg
     5701 ctggtaagga taaaggtaag cagggtcaag tagttcgagt gttgggtgat aaagttgttg
     5761 ttgagggcgt taatgttgta aaacgccatc aaaaacctaa tccaatgcgt ggcattgagg
     5821 gcggtattat tactaaagaa atgcctttgg atatttctaa tatcgcaatc ctgaatccgg
     5881 aaactaataa agcggaccgt gttggtatta agctgattga aaatgaaggc aaagttaaac
     5941 gcgttcgttt cttcaaatca aatggctcta tcattggggc ataaggagat aacatggctc
     6001 ggttgagaga gttttataaa gagacagttg ttcctgaatt ggttaaacaa tttggttaca
     6061 aatcagtaat ggaagtcccg cgtattgaaa aaatcacctt gaatatgggt gtgggtgagg
     6121 ctattgctga taaaaaagtt atggaacatg ctgtttccga tttagagaaa attgccggtc
     6181 aaaaaccggt tgttactgtt gcccgtaaat ctatcgcagg ttttaaaatc cgtgataact
     6241 atccggttgg ttgcaaagta acattgcgtc gtgatcaaat gtttgaattc ttggatcgtt
     6301 tgattactat tgcattacct cgcgtacgtg acttccgtgg tgtgagcggt aaatcatttg
     6361 atggccgtgg caattacaat atgggtgttc gtgagcaaat tatttttccg gaaattgaat
     6421 acgataaaat tgatgctttg cgtggtttga atattactat tactactaca gcaaaaaccg
     6481 atgaggaagc gaaagcttta ttgtcattgt ttaaatttcc gttcaaagga taatcatggc
     6541 taagaaagca cttattaatc gtgatctgaa acgtcaagct ttggctaaaa aatatgcggc
     6601 taaacgcgcg gcaattaaag cggtaatcaa tgattcgaat gcaactgagg aagagcgttt
     6661 tgaggctcgt ttgaggtttc aatccattcc tcgtaatgcg gcacctgtgc gtcaacgtcg
     6721 tcgttgtgct ttgacaggtc gccctcgtgg tactttccgt aaatttggtt tgggtcgtat
     6781 taaaatccgt gaaatcgcca tgcgtggcga aattccgggt gttgttaaag ccagctggta
     6841 ataggagtaa ttaagaatga gtatgcatga tcctatttcc gatatgttga ctcgtatccg
     6901 caatgcgcaa cgtgctaata aagcagcggt tgcaatgcct tcttcaaaat taaagtgtgc
     6961 tattgcaaag gtattgaaag aagaaggata tattgaggac ttcgcagttt catctgacgt
     7021 aaagtctata ttggaaattc aattaaaata ctatgcaggt cgtcctgtaa ttgaacaaat
     7081 caagcgtgta tctcgccctg gtttgcgtat ttataaagcg tctagtgaga ttccaagtgt
     7141 tatgaatggc ttgggtattg ctattgttag tacttctaaa ggtgtaatga ctgatcgtaa
     7201 agcacgttct caaggtgttg gtggtgagtt gttatgcatt gtagcctagt ggaggaaaag
     7261 aaatgtcacg tgtcgcaaaa aacccagtga ctgttcccgc tggtgtagaa gtaaaatttg
     7321 gaacagaggc attagttatt aagggtaaga acggtgaatt gtcttttcct ttgcattctg
     7381 atgtagccat tgaatttaat gatggcaaat tgacttttgt tgcgaataac agcagtaaac
     7441 aagcaaatgc aatgtctggt actgctcgcg cattagtcag caatatggtt aaaggtgttt
     7501 cagaaggttt tgagaaaaga ttgcaattga taggtgtggg ttatcgtgct caagcacaag
     7561 gtaaaatctt gaatctgtct ttgggttttt ctcatccgat cgtatatgaa atgcctgaag
     7621 gtgtctccgt tcaaactcct agccaaacag agattgtttt aaccggctcg gataaacaag
     7681 ttgttggtca agttgctgct gagattcgtg cgttccgtgc tcctgagcct tataaaggta
     7741 aaggtgttcg ctatgtaggc gaagtagtgg taatgaaaga agccaagaaa aaataattga
     7801 ggttcactaa tggataaaca tacaacccga ctccgtcgtg cacgcaaaac ccgtgctcgt
     7861 attgcggact tgaaaatggt aagattatgt gtgttccgaa gcaataatca tatttatgct
     7921 caagtaatta gtgctgaagg tgataaagta ttggctcaag cctctacatt ggaagctgag
     7981 gtgcgcggta gtctgaaatc tggaagcaat gttgaagcag ctgcaatagt tggtaaacgt
     8041 atcgctgaga aggctaaagc agcaggtgta gaaaaggttg cttttgatcg ttcaggtttc
     8101 caatatcacg gtcgtgtgaa ggctttggct gaagctgctc gtgaaaatgg tttaagcttc
     8161 taaatatttg gagactttca gatggcaaaa catgaaattg aagaacgcgg tgacggtctg
     8221 attgaaaaga tggtcgcagt taaccgtgta accaaagtag ttaaaggtgg tcgcattatg
     8281 gctttctctg cgctaactgt tgttggtgat ggagatggtc gtattggtat gggtaaaggt
     8341 aagtcaaaag aagtgcctgt tgcagtccaa aaggcgatgg atcaagcacg acgctctatg
     8401 attaaggtac cattaaaaaa tggtacgatt catcatgagg ttattggtcg acatggtgct
     8461 actaaagtat ttatgcagcc tgctaaagag ggtagtggcg taaaagccgg tggacctatg
     8521 cgtttggttt ttgatgctat gggcattcat aatatctccg ccaaagtgca cggatctact
     8581 aacccatata atatcgtacg tgcaacatta gatggtttgt ctaagttgca tactcctgct
     8641 gatatcgcag ccaaacgtgg cttgacagtg gaagacattt tgggagttaa ccatggctga
     8701 acaaaaaaag attagggtta cattggttaa aagcctgatt ggtacaattg aatctcatcg
     8761 tgcatgtgca cgcggtttag gtttgcgtcg tcgcgagcat acggtagagg ttttagatac
     8821 ccctgaaaac cgtggtatga ttaataaaat cagctacttg ttgaaagtgg agtcttgata
     8881 tgtttttgaa tacaattcaa cctgctgttg gtgctacgca tgctggtcgt cgtgttggac
     8941 gcggtattgg tagtggtctt ggcaaaacgg gtggtcgtgg tcataaaggt caaaaaagcc
     9001 ggtctggtgg gtttcataag gtgggtttcg agggtggtca aatgcccttg caacgacgcc
     9061 tccctaaaag aggttttaaa tctttaacag catcagctaa tgcacagctt cgtttaagtg
     9121 aactggaatc aattgctgtt aatgagattg atattttggt cttaaagcaa gcgggtctga
     9181 ttgcatctac agtctctaat gttaaagtta ttgcttctgg tgaaatttct aaggcagttg
     9241 ctttgaaggg tattaaagtt accaaaggtg cgagagctgc tatcgaggct gttggtggta
     9301 agattgaaat gtaaggttta atattgtggc taatcaacaa acgtcatcag gttcatccaa
     9361 atttggagat cttaagaaac gtcttttgtt tctatttgga gcattgattg tttttcgaat
     9421 tggtgcccat atacccgtac ctggagttga tgctgttgct ttagctaaat tatacgaaag
     9481 cgctggaaac ggcatcctgg gaatattgaa tatgttttcc ggtgggtcgt tagagcgctt
     9541 tagtatattt gcaataggaa ttatgccata tatttcagct tctattattg tacagctcgc
     9601 ttctgaaatt ttgccatcat tgaaggcttt aaaaaaagaa ggggaggctg gtagaaaggt
     9661 aattacgaaa tatactaggt atggtactgt tttgttagca attcttcaaa gtctaggtgt
     9721 tgcatctttc gtatttcagc aaggaattgt tgtaacaagt tcatttgagt ttcatgtttc
     9781 cacggtagtt tctttggtaa cgggaaccat gtttcttatg tggcttgggg agcaaattac
     9841 tgaaaggggt atcgggaacg gtatttcttt aatcattacg gcaggtattg cttcaggtat
     9901 tccttcgggt attgcaaagc tggttacact gacgaaccaa ggttctatga gcatgcttac
     9961 ggcgttgttt attgtatttg gtgccttatt attaatttat ttggttgtat actttgaaag
    10021 tgcacagcgg aagattccta ttcattatgc aaaacgccag tttaatggta gggcgggtag
    10081 tcaaaatacg catatgcctt tcaagttgaa tatggctggt gttattcccc caatttttgc
    10141 ttccagtatt attctatttc catctactct tttaggttgg tttggttcgg ctgatacaaa
    10201 tagtgttttg cacaaaatag ctggattgtt acaacacggt caattgctgt atatggcttt
    10261 atttgcagcg acagttattt tcttttgtta tttttatacg gctttggttt ttagccctaa
    10321 agaaatggca gagaatttaa aaaagagtgg tgcttttgtt cctgggatta gacctggtga
    10381 gcagacctct aggtatttag aaaaagttgt attacgtttg acattgtttg gagctcttta
    10441 tattacaact atttgtttaa ttccagagtt cttaactacg gttttaaatg taccttttta
    10501 tttgggtggc acgtctttgt tgattctagt tgttgtaacg atggatttta gtacacaaat
    10561 aaattcgtat aggcttactc aacagtatga taagttaatg actcgttcag aaatgaaatc
    10621 attttctcgg aaatagaatt atggcgaaag aagatactat ccaaatgcaa ggtgaaattc
    10681 ttgaaacttt acctaatgca acatttaaag taaaacttga gaatgaccat attgtattgg
    10741 gtcatatttc tgggaagatg cggatgcatt acattcgtat ttctccggga gataaggtca
    10801 cagtagagct gacaccttat gatctaacta gggctcgaat cgttttcaga gcaagataaa
    10861 ccaataaaag gaaaataaaa tgcgtgtaca accatctgtt aagaaaattt gccgaaattg
    10921 caagattatt cgtcgaaatc gtgtagttcg tgtaatttgt actgatctcc gtcacaaaca
    10981 gcgtcaaggt taatggaata tttcttttaa tgtgattctg tgatatagtg acacactttg
    11041 ccctaaaaag gaaaaaatat ggctcgtatt gcaggggtaa atatccctaa taacgcacac
    11101 atcgtaattg gtcttcaggc tatttacggt attggtgcta ctcgtgctaa attgatttgt
    11161 gaggctgcaa atattgcgcc tgatactaaa gcaaaagatt tggacgagac tcaattagat
    11221 gctttgcgtg accaagttgc caagtatgaa gtagaaggtg atttgcgtcg tgaggtaact
    11281 atgagtatca agcgattgat ggacatgggc tgctatcgtg gcttccgtca tcgtcgcggc
    11341 ttaccatgcc gcggtcaacg cactcgtaca aatgcgcgta cccgcaaagg tccgcgtaaa
    11401 gcgattgctg gtaagaaata aattttaagg aattttatta atggctaaag caaacacagc
    11461 ttcacgtgta cgtaaaaaag tacgtaaaac cgtgagtgag ggtattgtgc acgttcatgc
    11521 atctttcaac aataccatca ttacaatcac tgaccgtcaa ggcaatgcat tgtcttgggc
    11581 tacctctggc ggcgctggtt ttaaaggttc tcgtaaaagt acaccatttg cagcacaagt
    11641 tgcagcagaa gcagctggta aagttgccca agagtatggc gttaaaaatt tagaggttcg
    11701 tattaaaggt ccaggtccag gtcgtgaatc ctctgtacgt gctttgaatg ctcttggttt
    11761 caagattacc agcattactg acgttacccc gttgcctcat aacggttgcc gtccgcctaa
    11821 aaaacgtcgt atttaatatt ggagtgattt gaaacatggc acgttatatt ggccctaaat
    11881 gtaagttggc acgtcgcgaa ggtacggatt tgtttttgaa gagtgcgcgc cgctctttgg
    11941 attctaaatg taaaattgat tccgctcccg gtcagcatgg tgcaaaaaaa ccgcgtttgt
    12001 cagactatgg tttgcagttg cgtgaaaaac aaaaaatccg ccgtatttat ggcgtattag
    12061 aacgtcagtt ccgtcgttat ttcgcagaag ctgatcgtcg taaaggttct accggcgagt
    12121 tgctgttgca gttgctggaa tctcgtttgg ataatgtcgt ttatcgtatg ggtttcggtt
    12181 ctacccgagc tgaagcaaga cagcttgttt ctcataaggc gatagttgtg aatggacaag
    12241 ttgtcaatat tccttctttc caagtgaaag ctggtgatgt tgtctcagtt cgtgaaaaag
    12301 ccaaaaaaca ggtacgtatt caagaagcat tgggtttggc aactcaaatc ggcttgccgg
    12361 gttgggtttc tgtagatgcg gataaacttg agggtgtgtt caaaaacatg ccggatcgct
    12421 cggaattgac cggtgatatt aatgaacagc tggtggtaga gttctactct aaataatgct
    12481 agctcagtga gggacagtta aatgcagaat agcacaaccg aatttttgaa acctcgtcaa
    12541 attgatgtaa atactttttc tgcaactcgt gcaaaagtat ctatgcagcc atttgaacgt
    12601 ggtttcggtc ataccttagg taatgctttg cgccgtatct tactgtcatc catgaatggt
    12661 tttgctccta ctgaagtagc tattgccggt gtattacacg aatattctac tgttgatggt
    12721 attcaggaag atgttgttga cattttgctg aatattaaag gtattgtgtt taaactccat
    12781 ggtcgtagcc aagttcaact tgtgttgaag aaatcaggtt caggtgtcgt atctgccggt
    12841 gatattgagt tgccgcatga tgtagaaatt ctgaatcctg gtcatgtcat ttgtcatttg
    12901 gctgataacg gtcaaattga gatggaaatt aaagtagagc aaggtcgtgg ttatcaatct
    12961 gtttcaggtc gtcaggtagt tcgtgatgag aaccgtcaga ttggtgcaat ccagttggat
    13021 gcgagctttt cgcccatcag ccgtgttagc tttgaggttg aacctgcacg tgtagagcag
    13081 cggacggatc ttgataagtt ggttttggat atcgaaaccg acggttctat tgatcctgag
    13141 gaagctgtac gcagtgcggc acgtattttg attgatcaga tgtctatttt tgctgatttg
    13201 cagggtactc ctgtggagga ggttgaagaa aaagcacctc ctatcgaccc tgttcttttg
    13261 cgtccggtgg atgatctgga attgacagta cgttcagcta attgtttgaa agctgaggat
    13321 atttattata ttggcgattt gattcaacgc actgaaaccg agcttcttaa aacgccgaat
    13381 ttgggacgta aatctttgaa tgagattaag gaagtattgg catctaaagg tttgacactg
    13441 ggttctaagt tggaagcatg gccacctgta ggcttggaaa agccttaatg aagaattaaa
    13501 ggataattga tatgcgtcat cgtaatggca atcgcaaatt aaaccgtacc agcagtcatc
    13561 gtgctgcaat gctgcgtaat atggcgaatt cattattgac tcacgaagct attgtaacaa
    13621 ctctgcctaa ggccaaggaa ttgcgccgtg tagtagagcc gttgattaca ttgggtaaaa
    13681 agccgtcatt ggcaaaccgc cgtttggcat ttgaccgtac tcgcgaccgt gatgttgtag
    13741 taaaactgtt tggcgatttg ggtcctcgtt ttactgctcg taacggtggt tatgttcggg
    13801 tgttgaaata cggattccgt aaaggtgata atgcaccttt ggcactggtt gaattggttg
    13861 acaaaccggc tgctgagtaa ttttagtcat ataacgccat ctgccgaaaa gcaggtggcg
    13921 ttatttttgc aatatctgat aggtaatagg gtattggcta tcatgtttaa aatattaatt
    13981 gaatagctaa ggtttgcgcg gtaaacttac atcattaaaa aattctatga tggtttatat
    14041 aatgaatgct ttcgatataa agtcgacaaa gatggacgta ttgtctatat ctttgcatac
    14101 gtcagacttg tttgatttgg aagatgtgct ggtcaaattg ggcaagaagt ttcaagagtc
    14161 tggtgttgtt ccatttgtgc tggatgttca agagtttgat tatcccgagt ctttggatct
    14221 tgctgcattg gtttcgttgt tttcaaggca tggtatgcaa attttgggtc tgaagcattc
    14281 taatgaacgt tgggctgctg cggctatgaa gtatcatttg ctgttttgtc tgtctcattc
    14341 ggaaaatgtt aaagaactgg gtcaggttga ggtgcagaac acggaggatg gtcagaaagc
    14401 aaggaaaaca gtattgatta catcccctgt ccgtaccggt cagcaggttt atgccgaaga
    14461 tggcgatttg attgttacgg gggcggtcag ccagggggcg gaattgattg cagatggcaa
    14521 tatgcatatt tatgcgccga tgagagggcg tgctttggcc ggtgccaaag gcgatacttc
    14581 tgcccgcata tttatccact ccatgcaggc agaactggtt tctgtggcgg gtatttaccg
    14641 taattttgaa caggatttgc cggaccatct gcacaagcag ccggtacaga tattgttgca
    14701 ggataaccga ttggttatca gtgcaattgg ctcagaataa ttgtttgata ttttaaaaag
    14761 gaaatattgt ggcaaaaatt attgtagtaa cttcaggtaa gggcggtgtc ggtaaaacga
    14821 ctaccagtgc cagtattgcg acaggtttgg cattacgcgg atataaaact gcggtaattg
    14881 attttgatgt gggtttgcgt aacctcgacc tcattatggg ttgcgagcgt cgtgtcgttt
    14941 atgacctgat caatgtcatt cagggggagg cgacgctcaa ccaagctttg attaaagata
    15001 aaaattgtga aaacctgttt attttgccgg cttcccagac tcgggataaa gacgctttga
    15061 cacgcgaggg cgtagaaaaa gtgatgcagg agctgtccgg caagaaaatg ggctttgagt
    15121 atattatttg cgactctcct gccggtattg agcagggtgc attgatggcg ttgtattttg
    15181 ctgatgaagc cattgtaacg accaatcctg aggtttccag tgtgcgtgac tccgacagga
    15241 ttttgggaat tttgcaaagc aaatcccata aggcagagca aggcggttcg gttaaagaac
    15301 atctgttgat tacgcgttat tctcccgaac gtgtggcaaa aggcgaaatg ctgtctgtac
    15361 aggatatttg cgatattctg catatccctt tgctgggtgt gattcctgaa tcccaaaacg
    15421 tcttgcaggc atccaattcc ggagaaccgg tcatccatca ggacagcgtg gcggcttccg
    15481 aggcatataa ggacgttatt gcccgtcttt tgggcgagaa ccgtgaaatg cgtttcttgg
    15541 aagctgagaa aaaaagcttc ttcaaacgtc tgtttggagg ataaggtatg tcattaatcg
    15601 aatttttatt cggcagaaag cagaaaacgg caaccgttgc ccgcgaccgc cttcaaatca
    15661 tcattgccca agagcgcgcc caagaaggtc aggctccgga ttacctgccg actttacgta
    15721 aagagttgat ggaagtcctg tccaaatatg tgaatgtttc attagacaat atccgtattt
    15781 cccaggaaaa acaggatggt atggatgtgc ttgagttgaa cattactttg ccggaacaga
    15841 aaaaggtata ggacatgacc ttaaccgaat tgcggtacat cgtcgcagtc gcccaagaac
    15901 gtcatttcgg cagggcggcg cggcgttgtt ttgtcagcca gcccactttg tctattgcca
    15961 ttaagaaatt ggaagaagag cttgccgtct ctttgtttga ccggagcagc aacgatatta
    16021 ttacgaccga ggcgggggaa cgtatcgttg cacaggcgcg taaggtgttg gaagaggcag
    16081 agcttatcag gcatttggca aatgaagaac aaaacgagct ggagggtgcg ttcaaactcg
    16141 ggctgatttt tacggttgcg ccgtacctgc tgccgaaact gattgtctcg ttgcgccgta
    16201 ctgcaccgaa aatgcctttg atgttggaag agaattacac gcatactttg accgagtcgc
    16261 tcaaacgcgg ggacgttgat gcgattatcg ttgccgaacc gtttcaagag ccgggcattg
    16321 ttaccgaacc cttgtatgac gaaccgtttt tcgtgattgt cccgaaaggg cattcatttg
    16381 aggaactgga tgccgtttcg ccccggatgc tgggtgagga gcaggttttg ctgctgacgg
    16441 aaggcaactg tatgcgggat caggtactct caagctgttc cgaattggcg gcgaaacaac
    16501 gtatacaggg gttgaccaat acattgcagg gcagctcgat taatacaatc cgccatatgg
    16561 ttgccagcgg tttggcaatc agcgtgttac cggcaaccgc gctgaccgaa aacgatcata
    16621 tgctgttcag cattattccg tttgaaggta cgccgccaag ccggcgggtc gtattggcgt
    16681 accgccgcaa ttttgtccgt ccgaaggcgt tgtcggcgat gaaggcggcg attatgcagt
    16741 cgcagcttca cggggtaagt tttatccgcg actaggcgca ggcattgttt tcaaaacgcc
    16801 atttccctga gccgacaaca cggtatgcca agatattgcc gtcatcatcg attttgagta
    16861 tagcatcgcc acggaaactg ccgtcctgaa gatattcgac ttttgcatca ctgtgaatgt
    16921 tttcatcagt gccgatgcaa tgccatgtat agtggattaa caaaaaccag tacggcgttg
    16981 cctcaccttg ccgtactatt gccaaggcgt accagcctga tttgttgatg tgtgtactac
    17041 tgcatgggtt gttaagccct gcaagtgatt taagtgtgaa gattgagtaa tgcccactag
    17101 tcgcaggcgc ggggggcagg tgctaagtca ttggttatct ccttactatc tatctaacta
    17161 cagggtgtat tatgcgcctc cttgttgttt tgtgcaagtg gttttctgat aatttatgta
    17221 aagatttggt aaagacgtat agcctttttc tttgcattgt gcttgcgcag ggtatcaggt
    17281 aggtctcaaa aaatttgaaa atttttatgt tttcgtataa aatcttttgc acttatacgg
    17341 agtgagttat gcggtacacc attaaaacat ttcaaaccct tgctgatgaa actttcccct
    17401 ctaagaactt caaaattctt tcgttcaatg gggtggcacg acctataacc attgtttgcc
    17461 ctacacacgg ggtgcagact gtttctgcgg ggcaagcatt tatccgttcc aaacacggct
    17521 gtcctgcctg tggtttagtt ttatcaacct ccgttttggc ctctaggggt cggtcagtct
    17581 ccatcctcga caccgccaca ggcgaaacac tctcgttccc cagcgtacaa gctgccgcca
    17641 aagccctaaa cacctcctac ggctctatac gaaccaagct cgacggacgt tcgtcccccg
    17701 acaaccttgt ctgtaacagg tacaaagtaa tgccgtaatc tcgaaacttc gtgctataat
    17761 aaactcctag cttttgcagg agtttgtttt ttatggattt aaatgtcgaa atcaagaatt
    17821 ttggcaaggt tgcccatgct agggtttcca ttcgcccgct taccgtcatc acgggttgca
    17881 acccacactc gcgttcccca gcgttactgc cgcaggcact gctttgggtg tgcattttca
    17941 gcaaattaac cacaggctca agggtcgaac gtcctgtcag gaggtgttga tttctacacc
    18001 gattatactg tctgcggctt cgtcgccttg tcctgatttt tgttaatcca ctataaaaga
    18061 ggccgtctga aaaacatttt tcagacggcc ttgttgattc aatcaaatca gtctttcaac
    18121 ttcgacaact gagtttgcac ttttgccatt ttgtcttcca attccgccaa atcggctttg
    18181 tctttttcca ccaaatgcgc cggggctttt tcggtgtagc cgggtttgga gagtttggcg
    18241 ttgagtttgt ccaaggcttt ttgcagcttc tcggcttctt tgctcaaacg ggcggtttcg
    18301 gcggctttgt cgatttcgac tttcagcatc aggcgcgcgc cgttgcagac ggcgacgggc
    18361 gcgtcttcgc tttcgggtag ggcggcgact tgccgtgctt cggtcagtcg ggtcatcatc
    18421 ggcaggtatt tgaggtagtc cgccaagtcg tctgcgcttt cgacaaacag cggggctttc
    18481 acgttgggct ggatgcccat ttcgccgcgc aggttgcgga ctgcgccaat caaatcctgt
    18541 aacacggtca tttgcccgaa tgccgtctga acaatctcgc cgccgtcggt ttcggggaag
    18601 cgggcgagca tgatgctgtc ggcggttttc gcgtcgcaca taggagcgac ggtttgccac
    18661 agttcttcgg tgatgaacgg gataatcggg tgcagcaggc gcagggcggc ttcgagtacg
    18721 cgcaataagg tatggcgtgt ggcgcgttgg cggctggcgc agccggtttg gagctgcact
    18781 ttggcgagtt ccaaatacca gtcgcaatag tcgttccaca cgaagctgta cagggtttcc
    18841 gccgccaaat caaagcggta ggtttcgtag gcttgcgtaa cctgctcgat ggtctgattc
    18901 agacggccta caatccacat atcggggaag gagtagccgc gcggttcggt ggcggttgcg
    18961 ccgtagccgc agtcttggtt ttcggtgttc atcaagacga agttggtggc gttccagatt
    19021 ttgttgcaga aattgcggta gccttcggcg cgtttgaagt cgaagttgac gctgcggccg
    19081 aggctggcgt agctcgccat agtgaagcgc aatgcgtccg cgcccatact cggaatgcct
    19141 tcggggaaga gttttttggt ggcttcttcc actttcggcg cggtttcggg tttgcgcaga
    19201 ccggtcgtgc gtttcaccag tagcttgtcc aagtcgatgc cgtcaatcaa atcgacgggg
    19261 tcgatgacgt taccttcgga tttggacatt tttttgcctt catggtcgcg cacgatgccg
    19321 tggatgtaca cgtctttaaa cggtactttg ccggtgaagt gggtggtcat cataatcatg
    19381 cgcgccaccc agaagaagat gatttcgtag ccggtgacca agacgttgga cggcaggaag
    19441 gctttgagtt cgtcggtttc agacggccag ccgagcgtgg agaacggcac gagcgcggag
    19501 gagaaccatg tgtccaatac gtcttcttcg cgggtcaaac cggttttgcc ggcttgtttt
    19561 tcggcttccg cctgattgcg ggcgacgtaa acattgcctg cttcgtcata ccacgcgggg
    19621 atttgatgcc cccaccacag ttggcgcgag atgcaccagt cttggatgtt gttcatccat
    19681 tggttgtaag tgttgaccca gttttcaggg atgaagcgta ccgcgccgct gtcgacggct
    19741 tttttggctt tgtcggcgag gcttaaacct ttgaattcgc tgtcaggttc gccgccgttt
    19801 ggcgtggcgg acatggcgac aaaccattgg ctggtcagca tcggctcaat cactgagcct
    19861 gttcggtcgc ctttcggcgt catcagcgtg tgcgctttga tttcgaccaa caagccttgt
    19921 tcctgcaaat cggcaaccat ttgtttgcgc gcggcaaagc ggtccaagcc tgcgtatttt
    19981 tcaggcaggg caaagcctgg ttgcgcttcg cctttgaagt tgaacacttc ggcgtttgcc
    20041 agcactttgg cttccaagtc gaacacatta atcaggcgcg tgtcgtggcg tttgccgact
    20101 tcgtagtcgt tgaagtcgtg cgcgggcgtg atttttacgc agcctgtgcc gaagtctttt
    20161 tcgacgtatt cgtcggcaat cacgggaatg gtgcggccgg tcagcggcag gattaattcc
    20221 ttgccaatca aatgggtata acgttcgtct tcgggattga cggcaacggc aacgtcgccc
    20281 agcagcgttt cgggacgggt ggtggcgacg atgacggctt cggcaggatt gtccgccagc
    20341 ggatagcgga tgtgccacat agagccttgt tcttccacgc tttccacttc caaatcagac
    20401 actgcggtac ccaataccgg atcccagttc accaagcgtt tgccgcgata aatcaaacct
    20461 tgctcaaaca ggcgcacgaa cacttcggtt acggtttcgg cgcgcacgtc gtccatcgtg
    20521 aaatactcgc gcgtccagtc ggcagaacaa cccacgcggc gcatttgctg ggtaatcgta
    20581 ccgccggaaa cttctttcca ttcccacact ttttccaaga atttttcgcg ccccaagtca
    20641 tgacgggaca cgttttgcgc ggcaagctga cgctcgacca cgatttgcgt ggcgatgccc
    20701 gcgtggtcgg tgcccggaat ccaggcggtg ttgcagcctt tcatgcggta gtagcgggtc
    20761 aggccgtcca taatggtttg attgaaggcg tgtcccatat gcagcgtgcc ggttacgttg
    20821 ggcggcggca gttggatgga gaaagacggt ttggtcaaat ccatatcagg ttggaaatag
    20881 ccctgctctt cccagttttg ataatgtttg gattcgattt cggctggatt gtatttgtct
    20941 aacatgatgg aactttgtga aattaaggtt atttttgatg tgcggattat aacgcaaaaa
    21001 ggccgtctga atcatttcag acggcctttg gcatacaggt tttaaaaatg gaacaatacc
    21061 aggctgacgg caatcaccgc catgcccatt gtcaggccgt aaacggtttc atggccgtct
    21121 gaatagcgtt tggcagccgg cagcagctcg tccaacgcca aaaacaccat cacaccggct
    21181 atcacgccga ataccgaacc aaacacggca ggcgacaaaa acggctgcaa aaccaaatag
    21241 cccaaagccg cccccaacgg ctcggccaag ccggatagca gacacgccca caccgttttc
    21301 ttacggctgc gggtggcaaa ataaaccggc gcggcgatgg aaatgccctc cggaatatta
    21361 tggatggcaa tcgccaaggc caaaggcatc ccgactgctg gattttccaa tgtggcaaaa
    21421 aacgtcgcca agccttcggg gaaattgtgc gcagtaatcg caaacgccgc catcatgccg
    21481 actcgcgcga tatggcggcg tttgctttct tgaaacgacg ggtcttgcgc gtctaaagtt
    21541 tcatgcgggt tcggcaccag acggtcaatc agcgcaatgc cgcccatccc tgccaaaaat
    21601 gccatggtcg ccgccgcaaa cgcgtggtct ttatcataaa tttcagcgaa cgcctcgctg
    21661 gacttactga aaatctccgt cagggaaaca tacaccatcg caccgccggc aaatgccaaa
    21721 ccaaacgaca acacgcgcgg attgggcgtt ttggaaaaca tcaccaagcc gctgcctaat
    21781 acggtaaaca aaccggcagc caatgtgatg gaaaaggcaa cggccaaatt ggacatcgaa
    21841 aaatcgggca tgagaaaacc tgcgctaaaa gctgggacag gttcagacta acacttttta
    21901 atgtatatga taatagttat tatttatttt attgattgga tacacggatt ttgaaacaaa
    21961 agaccgtctg aaaaatgatt ttcagacggc ctttaaattt gaaatgccgc taaaccttag
    22021 tgctttccag cttaagcctg ataacgcgac aggctcaaat cgtcgctgcg gatttcggtg
    22081 tctttgccgc tcacgatatc ggcggttaat tttgccgaac ccagcgacat ggtccagcct
    22141 aaagtaccgt ggccggtatt cagaaacagg ttgtcaaagc gggtgcgacc gattaacggc
    22201 gtgctgtcgg gcgtcatcgg cctgaggccg ctccagaacg atgcttggct caaatcgccg
    22261 ccttccggga acaagtcgtt gacgaccaaa gccaaggttt cgcggcgttt ttcgggcagt
    22321 ttgatttcgt agcccgacaa ttccgccata ccgccgacgc ggattctgtt gtcaaagcgc
    22381 gtgatggcga ctttgtagct ttcatctaaa acggtggaca ccggtgcgcc gtcggaattt
    22441 gtgaccggca gggtcaagga atagcctttg acgggataaa tgggcagatt gagatccaac
    22501 tgcgccaaaa ccgtcctgct gaaacaaccg agcgcgcaga caacggcatc tgcttcaaac
    22561 cgccctgttt cggtttcaac ggttttgatg cgcagcccgt tgtggtcgat gcggctgatg
    22621 gtttggttga aatgaaaccg tacgcccttt tcctgacaca atttgtatag gttttcagtg
    22681 aagaggcggc agtcgccggt tgcatctgca ggcaggtgca ggccgctggc aattttggcg
    22741 gtaacgcgtg ccagcgcagg ctcaaattct gcacattctt cgggtttcag acggcggtac
    22801 ggcacgccgt agcgttccaa aacggcaatg tcttgttctg ccgcttcgac ttctttggtt
    22861 tggcggaaaa tctgcaacgt cccttttttg cgttcctcaa aattcatgcc ggtttgcgct
    22921 tcaaaacggc ggaacatttc acggctgtat tcggaaatcc tgaccatgcg ctctttattg
    22981 gtttgatagt gcgctgccgt gcagttttgc agcatttgcc acagccattc gatttgatac
    23041 aggctgccgt cggggcgaaa cagcaaaggc ggatggcttt taaacagcca tttcagcgct
    23101 ttggtcggga taccgggtgc agcccaaggc gtggtatagc cgtaagaaag ctggcctgcg
    23161 ttggcaaaac tggtttccat cgccacaccc tcggcgcggt cgatgaccgt tacttcatgt
    23221 cctgcctctg ccagatacca cgcggaagac acgccggcaa cacccgcacc taaaacaagc
    23281 actttcatgt ttctccctcc ggctttttca aaacagactt aatatgccgt gccgtctgaa
    23341 tattcggatt cagacggcct cggatattaa tgcggcaatt cgccgtttgt gattttttgt
    23401 ttgaagtcgc gcgtttcatt gacgatgact ttcgccatca ataaaagtgc aatcaggttg
    23461 ggcaatgcca tcaagccgtt gaatgtgtcc gaagccagcc acaccaaatc aaggctcaac
    23521 acggtaccta gcataacgga agaaacataa ccgacgcggt acaaaccggc aaatttctcg
    23581 ccgaaaacat acaccgcgca tttttcgccg taatagcacc agcccaaaat ggttgagtag
    23641 gcaaagaaaa tcaggccgat ggtgacaatc cagccgccga tgccgggcag cattttttgg
    23701 aatgtgacgg ttgtcagtgc cgcgccgctc acttcaggtt tgacaaactc gccgcccgcg
    23761 ccgagcagtc ccattaccaa cacgatgccg gtaatcgagc aaacgacgat ggtatccaaa
    23821 aacgtaccgg tcatagaaac caaggcctga cggacgggat ggtcggtttt cgcggctgcg
    23881 gcggcaatag gcgcagaacc catacctgcc tcattggaga acacgccgcg cgccacgccg
    23941 tagcggatga ccgtaccgat agcaccgccc gccactgcct gcgcgctgaa cgcatcggag
    24001 aaaatcagct tgacggcagg catcagtgca tcggaattaa tcgcgataat ggaaagaccg
    24061 cccaacacat aaaacaccgc catagcaggc acgatgaaag aagcggcttt ggcgatgcct
    24121 ttaataccac ctaaaacgac aacggcagtc agaacggtca acgtaatgcc ggtataggca
    24181 ggttcgatac cgaagctggt ttgcaccgcc tgtgcaaccg agttggactg caccgagctg
    24241 ccgataccga aggaagcgaa tgtgccgaac agcgcaaacg cgacggccat ccatttccag
    24301 tttttgccca agcctttttc gatgtaatac atcgggccgc cggacatttc gcctttggaa
    24361 ttgttgacgc ggtatttcac cgccaacacg ccttcgccgt atttggtggc catgccgaaa
    24421 atggcggtca tccacatcca aaataccgcg cccgggccgc cggttaccac cgcagtcgcc
    24481 acgccggcga tgttacccgt gccgatggtg gcggacagcg cggtcatcaa cgccgcaaaa
    24541 tgggaaatat cgccttcgtg gccttcgccg cttttatgct tctttggcgg cataaacgcc
    24601 tgtttcagcg cataacccaa catcgtgaac tgcaaacctt ttaataaaac agtcagcaaa
    24661 atacccgtgc cgaccagcag catcagcatc aaaggtcccc aaacccagcc gctgacggtt
    24721 tcaaaaaagg ctttgggatt gtctaaaaac acttgcatgg ctttctcctt tgtct
//
LOCUS       DAXHSF010000012        24664 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.012, whole genome shotgun sequence.
ACCESSION   DAXHSF010000012 DAXHSF010000000
VERSION     DAXHSF010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24664
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.012"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..1072
                     /locus_tag="ACFM8S_000398"
     CDS             <1..1072
                     /locus_tag="ACFM8S_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HFC6313522.1"
                     /translation="RPFFVGATVLAILGALVFFISPGAVVLHRQIFLELMLPAAYGGF
                     LTAALLDWTGFSGNLKPVATLMAALLLAASAILPFSPQTASFFVAAYWLVLLLFCARL
                     IWLDRNTDNFALLMLLAAFTVFQTAYAVSGDLNLLRAQVHLNMAAVMFVSVRVSILLG
                     AEALKECRLKDPVFIPNVVYKNIAITFLLLHAAAELWLSAQTAGFTSLAVGFILLAKL
                     RELHHHELLRKHYVRTYYLLQLFAAAGYLWTGAAKLQNLPASAPLHLITLGGMMGSVM
                     MVWLTAGLWHSGFTKLDYPKLCRIAVLLLFMAAVSRAFLMNVNPIFFITVPAILTAAV
                     FVLYLLTFVPIFRANAFTDDPE"
     gene            complement(1118..3307)
                     /locus_tag="ACFM8S_000399"
     CDS             complement(1118..3307)
                     /locus_tag="ACFM8S_000399"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFC6313523.1"
                     /translation="MIYHRIAVNVPLSDGLLTYSHSDPLPPGTRVLVPFRNKTVVGMV
                     WETDIAPDMDTARILSIQTTFLDEPPLPESWRDLLSFTSRYYHYPTGQAVFAALPQGL
                     KETRAVEMPQPPLFYALNEAGRAQTPPPARFNKKAALWDALLSGGMTMAALKQVNAQA
                     AKLIEDWTEQGWIETAEAAKPVLRSCHGQASHAGFVLNAGQRQASDEIQTALGRFQPF
                     LLYGITGSGKTEVYFDAMAKVLAQGRQVLFLLPEINLTPQLLKRVENRFADVPTAVLH
                     SQMAAGRRTQDYLRAMLGQAKLVIGTRLAVFTPMDDVGLIVVDEEHDGSFKQDNELRY
                     HARDLAVWRAKQGGCPIILGSATPSLESWHKAQSGAYRLLQLTERAHTAAQLPQVDIL
                     NVGRLKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCGDCGYTFGCPNCSA
                     KMVLHQRVRQLRCHHCDHREPIPFKCPDCGNQDLTAVGHGTQRVEETLRAFLPKAAVV
                     RVDRDSTAHKNDWADLYRRIADNEIDILVGTQMLAKGHDFARLNLVIVLNADGSLYSA
                     DFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYVVFAENELNE
                     RQMFAMPPFGFQTAVRADAPRVADAVEFLNAAKETLVPLLPESVSQFGAAPMLMVRLA
                     ERERAQIFLESPSRQDLHRAVSLWVQVLQQNRDGKIRWSVDVDPQEA"
     gene            3446..4228
                     /locus_tag="ACFM8S_000400"
     CDS             3446..4228
                     /locus_tag="ACFM8S_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFC6313524.1"
                     /translation="MKTKLIKILTPFTVLPLLACGQTPVSNANAEPAAKAESAGKSVA
                     ASLKARLEKTYSAQDLKVLSVSETPVKGIYEVVVSGRQIIYTDAEGGYMFVGELINID
                     TRKNLTEERAADLNKIDFASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRLEHEFEK
                     MTDVTVYSFMMPIAGLHPDAARKAQILWCQPDRAKAWTDWMRKGKFPVGGSICDNPVA
                     ETTSLGEQFGFNGTPTLVFPNGRSQSGYSPMPQLEEIIRKNQ"
     gene            complement(4546..6480)
                     /locus_tag="ACFM8S_000401"
     CDS             complement(4546..6480)
                     /locus_tag="ACFM8S_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HFC6313525.1"
                     /translation="MSLIECKNINRYFGSGENRVHILKDISLSIEKGDFVAIIGQSGS
                     GKSTLMNILGCLDTAGSGSYRIDGIETAKMQPDELAALRRERFGFIFQRYNLLSSLTA
                     RDNVALPAVYMGAGGKERSARADKLLQDLGLASKEGNKPGELSGGQQQRVSIARALMN
                     GGEIIFADEPTGALDTASGKNVMEIIRRLHEAGHTVIMVTHDPGIAANANRVIEIRDG
                     EIISDTSKNPEIPASNVGRIREKASWSFYYDQFVEAFRMSVQAVLAHKMRSLLTMLGI
                     IIGIASVVSVVALGNGSQKKILEDISSIGTNTISIFPGRGFGDRRSGRIKTLTIDDAK
                     IIAKQSYVASATPMTSSGGTLTYRNTDLTASLYGVGEQYFDVRGLKLETGRLFDENDV
                     KEDAQVVVIDQNVKDKLFADSDPLGKTILFRKRPLTVIGVMKKDENAFGNSDVLMLWS
                     PYTTVMHQITGESHTNSITVKIKDNANTQVAEKGLTDLLKARHGTEDFFMNNSDSIRQ
                     IVESTTGTMKLLISSIALISLVVGGIGVMNIMLVSVTERTKEIGIRMAIGARRGNILQ
                     QFLIEAVLICVIGGLVGVGLSAAVSLVFNHFVTDFPMDISAMSVIGAVACSTGIGIAF
                     GFMPANKAAKLNPIDALAQD"
     gene            complement(6546..7724)
                     /locus_tag="ACFM8S_000402"
     CDS             complement(6546..7724)
                     /locus_tag="ACFM8S_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacA family efflux pump subunit"
                     /protein_id="HFC6313526.1"
                     /translation="MAKMMKWAAVAAVAAAAVWGGWSYLKPEPQAAYITETVRRGDIS
                     RTVSATGEISPSNLVSVGAQASGQIKKLYVKLGQQVKKGDLIAEINSTSQTNTLNTEK
                     SKLETYQAKLVSAQIALGSAEKKYKRQAALWKDDATSKEDLESAQDALAAAKANVAEL
                     KALIRQSKISINTAESELGYTRITATMDGTVVAILVEEGQTVNAAQSTPTIVQLANLD
                     MMLNKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSSGGYNSSTD
                     TASNAVYYYARSFVPNPDGKLATGMTTQNTVEIDGVKNVLIIPSLTVKNRGGKAFVRV
                     LGADGKAAEREIRTGMRDSMNTEVKSGLKEGDKVVISEITAAEQQESGERALGGPPRR
                     "
     gene            7950..8339
                     /locus_tag="ACFM8S_000403"
     CDS             7950..8339
                     /locus_tag="ACFM8S_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HFC6313527.1"
                     /translation="MKNVQKGFTLLELMIAVAILGILTLITYPSYKTYIRRVRLSEVR
                     TTLLHNAQTMERYYRQKGTFENYDKNKLKQNKYFNITLSKVSPDHFTLQAAPDPMTNE
                     GETCIVTLNDGGTLSAAGSGQDCPDFD"
     gene            8622..9668
                     /gene="adhP"
                     /locus_tag="ACFM8S_000404"
     CDS             8622..9668
                     /gene="adhP"
                     /locus_tag="ACFM8S_000404"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFC6313528.1"
                     /translation="MKMQAVVVNKNVAGDVEIVEREVRPLEYGEALVEVEYCGVCHTD
                     LHVAAGDYGEKPGRVLGHEGIGLVKEVADGVKNLKVGDRVSIAWLFQSCGSCEYCNTG
                     RETLCRSVLNAGYTADGGMATHCIVNADYAVKVPEGLDPAQASSITCAGVTTYKAIKV
                     SSVRPGQWIAIYGAGGLGNLGVQYAKKVFGAHVVAIDINDDKLAFAKETGADLVVNAA
                     KEDAAKVIQEKTGGAHAAVVTAVSAAAFNSAVNCVRAGGRVVAIGLPPESMDLSIPRL
                     VLDGIEVVGSLVGTRKDLEEAFQFGAEGLVVPKVQLRALDEAPAIFQEMREGKITGRM
                     VIDMKKECSCGHHH"
     gene            complement(9833..13318)
                     /gene="smc"
                     /locus_tag="ACFM8S_000405"
     CDS             complement(9833..13318)
                     /gene="smc"
                     /locus_tag="ACFM8S_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFC6313529.1"
                     /translation="MRLTHIKLSGFKSFTDPTTIHVPGQLVAVIGPNGCGKSNVIDAV
                     RWVLGEASAKQLRGESMQDVIFNGAATRRPAPRASVELVFDNSDHSLQGAWGQYAEVS
                     IKRQLTRQGESTYFINNQTVRRRDITDLFLGTGVGARGYAVIEQGMISRIIEARPEEL
                     RAYIEEAAGVSKYKERRKETEGRLKDTREHLQRLGDLQNELARQVEKLEKQAETAERY
                     KSLTAQLNQQQDLLDYAQWQQSLAAADKATAQHQSLQAQQDETAAQVQALNDEVHALQ
                     TAEQSQQQAVHELSNKRGVLREQIARLEEQIRHRQNLHQRIERDKQAAQAQLQRIHQE
                     QQQIRVQLEENELQAEEKQTELAEWAMQVAEHEERLPELEEAQTALNAAFQTRQDEAN
                     RIRRELALKQQQLAHAEQTVAKHEERKGRLKQENQALNLPDEAETAAAQEAAALLQNR
                     QEHYEEQIIAAEEALHAAREAFQTASNRFQSLKQQHITLQAQQQALSQILSQQQEAAD
                     FWQASDHAAAPQLWQHITAPAEWQHALSVILAERLQARSVPHGFVPPAPLPQGQAAWL
                     SDGLSGGIKKSLPVQALLNQIQAQPPFQTALHHWLDGVLCAPDLSYALAHQNDLGAHQ
                     IWLTPEGHQVDKVSVLLYAKPAQESLIAQKAHLDGIASELDNLAPELSAAEAAFKQAE
                     AAVRSSEVQHKNLMQQQQQHTRQYSQAQQRAAELLARTNQGQIRREHIERELAQLAEE
                     QTVLQHTSDGLSDDIFTLQEAAAELEHQQQTAAHSRQEQQGRLKQAQLALLEANRQYG
                     LAEVAVHKLNQQKQNYQQQIAQLEQQTFDWQERQQELALAYETEFQNDEQHIKLEELS
                     EAVQSLDEEYIVVQEKLAQIQEQGREQYARVQTLQTKLPQLQAATQTALLQQQEALIN
                     AKRYHQNLTERAADLDALEALTQESPKVLNSSIGSLSQQIEALGAVNLAALQELEEAR
                     ERDGYYRSQSEDVQAAITLLEEAIAQIDDKTKARFKETFDAVNGKVQTFFPTLFGGGE
                     ATLKMIGDDLLTAGVSIMARPPGKKNSTIHLLSGGEKALTAMSLVFALFSLNPAPFCL
                     LDEVDAPLDDANTSRFCNLVKEMSAQTQFLYISHNRLTMEMAEQLVGVTMQEKGVSRV
                     VAVDIKQALEMAESV"
     gene            13302..13571
                     /locus_tag="ACFM8S_000406"
     CDS             13302..13571
                     /locus_tag="ACFM8S_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009345706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313530.1"
                     /translation="MWVRRIMVGKNKKEAYFNGNPAAPPHILPAKPYRIPHPRCRLKP
                     SDGIFNAPEPRFRQCPMLGAAFAVLPRRRARYNPPRHPPDLETCL"
     gene            13568..14290
                     /gene="lpxH"
                     /locus_tag="ACFM8S_000407"
     CDS             13568..14290
                     /gene="lpxH"
                     /locus_tag="ACFM8S_000407"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFC6313531.1"
                     /translation="MKPAYFISDLHLSEKHPELTALLLRFLRSSAAGQARAVYILGDL
                     FDFWVGDDEVSELNTSIVREIRKLSDKGVAVFFVRGNRDFLIGQDFCRQAGMTLLPDY
                     SVLDLFGCKTLICHGDTLCTDDRAYQRFRKIVHRKRLQKLFLMLPLKWRTRLAAKIRR
                     VSKMEKQVKPADIMDVNAAFTARQVRAFNAERLIHGHTHREHIHHENGFTRIVLGDWH
                     NDYASILRVDGDGAVFVPLEKY"
     gene            14559..16145
                     /locus_tag="ACFM8S_000408"
     CDS             14559..16145
                     /locus_tag="ACFM8S_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFC6313532.1"
                     /translation="MALFLSIFPIVLLIWLMVKKNSMPSYVALPITAVLIYAIKLFYF
                     DDAGMLLNATAASGLVKTLTPITVIFGAIMFNRMMETTGCIDVIRKWLATISPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILMSLGFNPLKVAIFTLVMNSVPVSFGAVGT
                     PTWFGFAPLNLSAEDILAIGRQTGVMHFFAGFVIPVIGLGFIVPWSEIRKNLGFVAIA
                     VFSCTIPYVALAMVNEEFPSLVAGAIGLMVSVFAANQGWGLSKDHAKDPNAEKVPFAQ
                     VAKALAPLGMLIGMLVVTRIKQLGIKGILTSKEEWFSFQLPFDLSKITVSDSLTITFG
                     NIFGQDVSASYQTLYVPAWIPFVLTVWICILLYKTKFKDAWTIYAVTFNQTKKPLLAL
                     MGALIMVQLMLVGGDNSMVKIIGKEFAAMAGEHWVYFSPYLGAIGAFFSGSNTVSNLT
                     FGPIQQQIALDTGLSVTLILALQSVGGAMGNMVCLNNIIAVCTVLDVKNSEGAIIKKT
                     VIPMAIYGVIAVVAAMIFFL"
     gene            complement(16314..16517)
                     /locus_tag="ACFM8S_000409"
     CDS             complement(16314..16517)
                     /locus_tag="ACFM8S_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HFC6313533.1"
                     /translation="MDNLNLQEISVLPENLPLYCSGPDNEQWNGHPRVFLPLCEGESG
                     SVACPYCGTRYRLDGKMPHHHYA"
     gene            16633..17130
                     /locus_tag="ACFM8S_000410"
     CDS             16633..17130
                     /locus_tag="ACFM8S_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-mimic protein DMP19"
                     /protein_id="HFC6313534.1"
                     /translation="MTALTLPEDIRQQEPSALLYTLVSAYLEHTAQTGDESLSCLSDD
                     QHTLTAFCYLDSQVEEGGFVQLIASGYGEYIFRNPLADSLRRWKIKAVPKVLDKAKAL
                     YEQHGKTIETLADGGADIESLRKQFPDFEEWDGAYYEAAEQDLPLLAEHIQSNWETFA
                     HIGQA"
     gene            17212..18405
                     /locus_tag="ACFM8S_000411"
     CDS             17212..18405
                     /locus_tag="ACFM8S_000411"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HFC6313535.1"
                     /translation="MFFKHIEAAPADPILGLGEAFKAETRPEKVNLGIGVYKDASGAT
                     PLVKAVKEAEKRLLESETTKNYLTIDGVADYNAQTQILLFGKDHEIIASRRAKTAQSL
                     GGTGALRIAAEFAKRQLNAQTIWISNPTWPNHNAIAKAVGIQDKPYRYYDAAKHGLDW
                     DGMIEDLSQAQKGDIVLLHGCCHNPTGIDPTPEQWETLAKLSAEKGWLPLFDFAYQGF
                     GNGLEEDAYGLRVFLKHNTELLIASSYSKNFGMYNERVGAFTLVAEDEETAARAHSQV
                     KTIIRTLYSNPASHGANTIALVLKNDDLKAQWIAELDEMRGRIKAMRQKFVELLKAKG
                     ASQDFDFIIEQNGMFSFSGLTPEQVDRLKNEFAIYAVRSGRINVAGITDDNIDYLCES
                     IVKVL"
     gene            complement(18809..19744)
                     /gene="hemC"
                     /locus_tag="ACFM8S_000412"
     CDS             complement(18809..19744)
                     /gene="hemC"
                     /locus_tag="ACFM8S_000412"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFC6313536.1"
                     /translation="MNPKKLVIASRESLLAMWQAKHIQGRLKALYPDCEVEILGMTTR
                     GDQILDKTLSKVGGKGLFVKELEQALYDGRADLAVHSIKDVPMDLPEGFALAAIGERA
                     NPFDAFVSNQYARLEEMPKGAVVGTSSLRREAQLRARYPHLVIKPLRGNVQTRLSKLD
                     NGEYDAIILAAAGLQRLELDERIRMILSESDSLPAAGQGALGIEIAAHREDLYEVLKP
                     LNHDTTHACVTAERALARALGGSCQVPLAAYCTEENGLLTLRGLVGHPDGSVVLRADA
                     QAPAEYADALGRAVAKKLADDGARELIGAVLNTEN"
     gene            19863..20378
                     /gene="ybeY"
                     /locus_tag="ACFM8S_000413"
     CDS             19863..20378
                     /gene="ybeY"
                     /locus_tag="ACFM8S_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFC6313537.1"
                     /translation="MKRTKKYPFLTLQRQRFHLNFENASSAAGIPAERDFYRWAWSAL
                     KSGYRRADISLILLDEEEARAYNRDYRGKDYATNVLSFALNEGEILPCQVSEKLYGDL
                     IICPQVVLKEAAEQGKTPERHFAHLTIHGVLHLMGYDHIEDDEAEIMEAEEIRLMLAA
                     GFPNPYQEDEY"
     gene            20380..21204
                     /locus_tag="ACFM8S_000414"
     CDS             20380..21204
                     /locus_tag="ACFM8S_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFC6313538.1"
                     /translation="MDGTQSKPKFFERLISRLAGEPDSAEDVLTLLRQAHEQEVFDAD
                     TLLRLEKVLDFSDLEVRDAMITRSRMNVLKENDSIERITAYVIDTAHSRFPVIGEDKD
                     EVLGILHAKDLLKYMFNPEQFHLKSILRPAVFVPEGKSLTALLKEFREQRNHMAIVID
                     EYGGTSGLVTFEDIIEQIVGDIEDEFDEDESADNIHAVSSERWRIHAATEIEDINTFF
                     GTEYSSEEADTIGGLVIQELGHLPVRGEKVIIGSLQFTVARADNRRLHTLMATRVK"
     gene            complement(21347..22726)
                     /gene="nhaC"
                     /locus_tag="ACFM8S_000415"
     CDS             complement(21347..22726)
                     /gene="nhaC"
                     /locus_tag="ACFM8S_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFC6313539.1"
                     /translation="MFAFKSLLDMPRGEALAVVVALIAAMGYTIISLEWLPHMSIIAA
                     IVVLILYGLARGLKYNDMQQGMIGALNQGMGAIYLFFFIGLMVSALMMSGAIPTLMYY
                     GFGLISPTYFYFSAFALCSVIGVSIGSSLTTCATVGVAFMGMAAAFHADMAMTAGAIV
                     SGAFFGDKMSPLSDTTGISASIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPSVAA
                     QDLNSVESFRSQLEATGLVHGYSLIPFALLVILALMRVNAVVAMLFTVMVAVAVTYLH
                     STPDLRQLGAWFYGGYKLEGEAFKDIAKLISRGGLESMFFTQTIVILGMSLGGLLFAL
                     GAIPSLLDAVRSFLTNAGRATFSVAMTSVGVNFLIGEQYLSILLSGETFKPVYDKLGL
                     HSRNLSRTLEDAGTVINPLVPWSVCGVFISHALGVPVWEYLPYAFFCYLSLALTLLFG
                     WTGLTLSKK"
     gene            complement(23017..24240)
                     /locus_tag="ACFM8S_000416"
     CDS             complement(23017..24240)
                     /locus_tag="ACFM8S_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HFC6313540.1"
                     /translation="MSAQSQNNHTSPLVVLTTLFFMMGFITCMNDILIPHLKEIFDLS
                     YVQAMLIQFCFFTAYAVMSIPMGAFVGKVGYKNGVIGGFLLTAVGCLLFYPAAGSHSY
                     AVFLGALFILASGVTLLQVAGNPYVTLLAKPGKESATLTLVQAFNALGTTIAPQIGAF
                     LILADATQTVSKAEQISSVQIPYLGLAGLLIILAVFVKMIRLPDARKIAAEESAHNHD
                     GKTSVWQYKHLVFGTAGIFCYVGAEVSIGSLMVNVLGYLKGLDHASAAHYLSFYWGGA
                     MVGRFLGSAVMAKFAPNRYLAFNASAAVVLLAVAMATGSGNADVAMWSLLAIGFFNSI
                     MFPTIFSLATKGLGKFTNAASGVLCTAIVGGAVVPVVQGWVADTYTLMSSFVVSVICY
                     LYIVFFAVYGYRADK"
BASE COUNT         5636 a         7060 c         6380 g         5588 t
ORIGIN      
        1 gcgcccgttt ttcgtcggcg cgacagtgct tgccatactc ggcgcgttgg tgtttttcat
       61 cagccccggt gccgtcgtcc tgcaccgcca aatcttctta gaactcatgc tacctgcggc
      121 atatggtggt ttcctgactg cggctttgtt ggactggacg ggtttttcgg gtaacctgaa
      181 acctgtcgcg actttgatgg cggcattatt gctcgccgca tccgctatac tgcccttttc
      241 gccgcaaact gcctcgtttt tcgtcgccgc ctattggctg gtgttgctgc tgttctgcgc
      301 ccggctgatt tggctagacc gaaacaccga caacttcgcc ctgctaatgt tacttgccgc
      361 gttcactgtt tttcagacgg catatgccgt cagcggcgat ttgaacctgt tgcgcgcgca
      421 agtgcatcta aatatggcgg cggtgatgtt cgtatccgtg cgcgtcagta ttcttttggg
      481 cgcggaagcc ctgaaagaat gccgtctgaa agacccagta ttcatcccca atgtcgtcta
      541 taaaaacatc gccattacct tcctgctcct gcacgccgcc gccgaacttt ggctgtctgc
      601 gcaaaccgcc ggttttacct cgctcgccgt cggctttatc ctgcttgcca agctgcgtga
      661 gcttcaccat cacgaactcc tgcgcaaaca ctacgtccgc acttattacc tgctccaact
      721 ctttgccgcc gcaggctatt tgtggacagg cgcggcgaaa ttacaaaacc tgcccgcctc
      781 cgcgcccctg cacctgatta ccctcggtgg catgatgggc agcgtgatga tggtgtggct
      841 gactgccgga ctgtggcaca gcggctttac caagctcgac tacccgaaac tctgccgcat
      901 cgccgtcctc ctgctcttta tggctgccgt ctcgcgtgct ttcttgatga acgtgaatcc
      961 aatattcttc atcaccgtcc ccgcgattct gaccgccgcc gtgttcgtgc tttacctgct
     1021 gacattcgta ccgatctttc gggcgaacgc gtttacagac gatccggaat aaaaatgccg
     1081 tctgaaacgg tttcagacgg catcgtattg ccgataatca agcctcctgc ggatcgacat
     1141 ccaccgacca tctgattttg ccgtccctgt tttgctgcaa cacctgcacc cacaaactca
     1201 cggcgcggtg caaatcctgt cgggacgggg attcgaggaa gatttgcgcg cgttcgcgtt
     1261 cggcgaggcg caccatcagc atcggggcgg cgccgaactg tgaaacgctt tcgggcaaca
     1321 gcgggaccag ggtttctttg gcggcgttga gaaactccac cgcatcggca acgcgcggcg
     1381 cgtcggcgcg gacggcggtc tggaaaccga agggcggcat ggcgaacatt tgccgctcat
     1441 tcaattcgtt ttcggcaaac acgacgtagt cctgcgcttt gacggcggcg aagacgggat
     1501 gttcgggcag ttgggtctgt atcagcacct tgccgggctt gtcggcgcgc cccgccctgc
     1561 cggacacctg catcagctcg gcaaacagcc tttccggcgc gcggaaatcc gcgctgtaca
     1621 ggctgccgtc ggcgttcaat acgataacga ggttgagccg cgcgaaatca tgacctttgg
     1681 cgagcatctg cgtgccgacc agaatatcga tttcgttgtc ggcgatgcgg cggtacaaat
     1741 ccgcccagtc gtttttgtgc gccgtactgt ccctatcgac gcggacgacg gcggccttgg
     1801 gcaggaaagc gcgcagggtt tcttcgacgc gctgcgtgcc gtgtccgacg gcggtcaggt
     1861 cttgattgcc gcagtcgggg catttgaacg gtatgggttc gcggtggtcg cagtggtggc
     1921 agcgcagttg gcggacgcgt tggtgcagca ccattttggc ggagcagttc gggcagccga
     1981 aggtataacc gcagtcgccg caaaacagcg cgggcgcgaa gccgcgacgg ttgaggtaca
     2041 ccaacgacat gccacctgct tcaaagttct gtttcaaaag ctgcaaggct tgcggcgaga
     2101 agccgttgtc aagtttcaga cggcctacgt tgaggatgtc cacttgcggc agttgcgcgg
     2161 cggtatgggc gcgttcggtc agttgcagca ggcggtacgc gccgctttgc gccttgtgcc
     2221 agctctccaa gctgggggtg gcactgccca atatgatcgg gcagccgccc tgcttcgccc
     2281 gccacaccgc caaatcgcgg gcgtggtagc gcaattcgtt gtcctgtttg aacgagccgt
     2341 cgtgttcctc atcgaccaca atcagcccga catcatccat cggcgtgaac accgccaacc
     2401 gcgtgccaat caccagcttc gcctgcccca acatcgcgcg caaataatcc tgcgtgcgcc
     2461 tgcctgccgc catctggctg tgcaacacgg cggtcggcac gtcggcaaaa cggttttcta
     2521 cccgcttcaa aagctgcggc gtgaggttga tttcgggcaa cagaaacaac acctgccgcc
     2581 cctgcgccaa cactttcgcc atcgcatcaa aatacacctc ggtcttgccg ctgccggtga
     2641 tgccgtacag caaaaacggc tgaaaccgtc ccaatgccgt ctgaatctca tcggaagcct
     2701 gccgctggcc ggcgttcaac acaaatccgg cgtgcgaagc ctgcccgtgg cacgacctta
     2761 aaaccggttt cgccgcttcc gccgtttcaa tccagccctg ctccgtccaa tcttcgatta
     2821 atttcgccgc ctgcgcgttc acctgcttca acgctgccat cgtcattccg cccgaaagca
     2881 gcgcgtccca caaagccgct tttttgttga accgcgccgg cggcggcgtt tgcgccctgc
     2941 ccgcttcgtt caacgcataa aacaacggcg gctgcggcat ttccaccgcg cgcgtttcct
     3001 tcaaaccctg cggcagcgcg gcaaacaccg cctgccccgt cggatagtgg taataacgcg
     3061 acgtaaacga gagcaaatca cgccagcttt caggcaacgg cggctcgtct aaaaaggtcg
     3121 tctgaatact caaaatccgc gccgtatcca tatccggcgc aatatccgtt tcccacacca
     3181 tcccgaccac ggttttattg cggaaaggca caagcacccg cgttcccgga ggaagcggat
     3241 cggaatggga ataagtcaaa aggccgtctg aaagcggcac gtttacggcg atgcggtggt
     3301 agatcataca ggcaggcaaa tcattttttt gcgtatttta acaggcggaa gctattttca
     3361 gacgataaat tgagcctagg cgcaatgcag gcgcaacaga gatttgatac aattccgcct
     3421 ttccaacacg cgcaatcatc tttatatgaa aaccaagtta atcaaaatct tgaccccctt
     3481 taccgtcctc ccgctgctgg cttgcgggca aacgcccgtt tccaatgcca acgccgaacc
     3541 cgccgccaaa gccgagtccg ccggcaaatc cgttgccgcc tctttgaaag cgcgtttgga
     3601 aaaaacctat tccgcccaag atttgaaagt gttgagcgtt agcgaaacac cggtcaaagg
     3661 catttacgaa gtcgtcgtca gcggcagaca gattatctac accgatgccg aaggcggcta
     3721 tatgttcgtc ggcgaactca tcaacatcga cacgcgcaaa aacctgaccg aagaacgcgc
     3781 cgccgatttg aacaaaatcg acttcgcctc cctgcctttg gacaaagcca tcaaagaagt
     3841 gcgcggcaac ggcaagctga aagtcgccgt cttttccgac cccgattgtc cgttctgcaa
     3901 acgcttggaa cacgagtttg aaaaaatgac cgacgtgacg gtttacagct ttatgatgcc
     3961 cattgccggc ctgcaccctg atgccgcgcg caaggcgcaa atcttatggt gtcagcccga
     4021 ccgcgccaaa gcgtggacgg attggatgcg taaaggcaaa ttcccggtcg gcggcagcat
     4081 ctgcgacaat cccgtcgcgg aaaccacttc cttgggcgaa caattcggct tcaacggcac
     4141 gccgaccctc gtcttcccca acgggcgcag ccaaagcggc tacagcccga tgcctcaact
     4201 ggaggaaatc atccgcaaaa atcaataatc catccgcaaa acaaaaggct tggaaaattc
     4261 caagcctttt ttgcgggata tgccgcctga aaaccaatcc gcactgaaag ttatagtgga
     4321 ttaaatttat agtggattaa atttaaacca gtacagcaag ccaacaaaca catcctaacc
     4381 cattgaatga ttgaaaataa taaattgaac aatacgaaac acaccgccgt cattcccacg
     4441 caagtgggaa tctggaacgt agaatctaag aaaccgtttt tgtccaataa gtttctgctc
     4501 caaaatgacc ggttctgcag cttcaggcgg catctttgtc caacctcaat cctgcgccaa
     4561 tgcgtctatc ggattgagtt tggccgcctt gttggcaggc ataaagccga acgcgatgcc
     4621 gattccggtc gaacaggcga ccgcgccgat gacggacatg gcggaaatgt ccatcgggaa
     4681 gtcggttaca aaatgattga acacgaggct gacggcggcg gacaaaccca cgccgaccaa
     4741 accgccgatg acgcagatta acaccgcctc aatcaaaaac tgctgcaaaa tattgccgcg
     4801 ccgcgcgccg attgccatcc gtatgccgat ttctttggtg cgctcggtaa cggacaccag
     4861 catgatgttc atcacgccga tgccgccgac taccaatgaa atcagggcga tggaggaaat
     4921 cagcagcttc atcgtaccgg tggtgctttc gactatctgc ctgatgctgt cgctgttgtt
     4981 catgaagaaa tcttccgtgc cgtgccgcgc tttgagcaga tcggtcagcc ctttttcggc
     5041 aacctgggta ttggcattgt ctttgatttt gacggtgatg gagttggtgt ggctctcgcc
     5101 tgtgatttgg tgcatcaccg tcgtataggg cgaccaaagc atcagcacgt cggaattgcc
     5161 gaaagcgttt tcgtcttttt tcatcacgcc gatgacggtc aaggggcgtt tcctgaacaa
     5221 aatggtttta cccaacggat ccgagtccgc aaagagtttg tctttgacat tttggtcgat
     5281 gacgacgacc tgcgcgtctt ctttcacatc gttttcgtca aacagccgcc ccgtttccag
     5341 cttcagtccg cgcacgtcga aatattgttc gcccacgccg tacaaagaag cggtcaggtc
     5401 ggtattgcgg taagtcagcg tgccgccgct cgaagtcatg ggcgtggcgg aagcaacgta
     5461 gctttgtttg gcgatgattt ttgcgtcgtc tatggtcagg gttttaatcc tgccgctgcg
     5521 cctgtcgccg aagccgcgcc ccgggaagat gctgatggtg ttcgtcccta tcgaactgat
     5581 gtcttcaagg atttttttct gcgaaccgtt gcccaatgcg acgacggaaa ccaccgacgc
     5641 gatgccgatg atgatgccga gcatcgtcag aagcgaacgc attttgtgcg ccaatactgc
     5701 ttgcaccgac attctgaagg cttcgacaaa ctggtcgtaa taaaacgacc acgaggcttt
     5761 ttcccgaatc ctcccgacat tgcttgcggg gatttcggga tttttcgagg tgtcggaaat
     5821 gatttcgccg tcccggattt cgatgacgcg gttggcattg gcggcgatgc cggggtcgtg
     5881 cgtgaccata atgacggtat gcccggcttc gtgcagcctg cggatgattt ccatcacgtt
     5941 tttgccgctg gcggtatcga gcgcgccggt cggctcgtcg gcgaagatga tttctccgcc
     6001 gttcatcagg gcgcgggcga tggagacgcg ctgctgctgt ccgcccgaga gttcgccggg
     6061 cttgttgccc tctttgcttg ccaaacccaa atcctgcaag agtttgtccg cccgcgcgga
     6121 acgctctttg ccgcccgcgc ccatatagac ggctggcagc gcgacgttgt cccttgcggt
     6181 cagcgagctt aagaggttgt agcgttggaa gatgaaaccg aagcgttcgc ggcgcaatgc
     6241 cgccagctcg tcaggctgca ttttggcagt ttcgatgccg tcgattcggt acgaaccgga
     6301 accggcggta tccaaacagc cgaggatgtt catcagcgtg gacttgcccg aaccggactg
     6361 cccgatgatg gcgacaaaat cgcccttctc tatcgacagg ctgatgtctt tcaaaatatg
     6421 gacgcggttc tcgccgctgc cgaaatagcg gttgatgttt ttacattcga tcaagctcat
     6481 aatattccgt aaatcaataa caaatgccgt ctgaaaccgt ttccgtgttc agacggcata
     6541 ttcgtttatc ggcgcggcgg gccgcctagg gcgcgttcgc cgctttcctg ttgctcggcg
     6601 gcggttattt cggagatgac cactttgtcc ccctctttca acccgctttt tacttcggta
     6661 ttcatactgt ctctcatacc ggtccggatt tcgcgttccg ccgccttgcc gtccgcaccc
     6721 aacacgcgca caaacgcctt gccgccgcga tttttcacgg tcagcgacgg aataatcagc
     6781 acatttttca cgccgtcgat ttcaaccgta ttctgcgtcg tcatccccgt ggcgagtttg
     6841 ccgtccggat tcggcacaaa cgaacgggca taatagtaga ccgcattgga agccgtatcc
     6901 gtactgctgt tgtagccgcc cgacgacatc gtggtcagcc cggggtcgac gctgtcgagc
     6961 ttcgccttaa tcggcgtatc cggttcggac aaaatcgtaa acgaaatatc ctgccccgcc
     7021 ttcaccttgg taatatcgcc ctcggcaatc tgcattttgt tcaacatcat atccagattc
     7081 gccaattgga caatcgtcgg cgtagactgc gccgcgttca cagtctgccc ctcttccacg
     7141 agaatcgcta ccaccgtgcc gtccatcgtt gcggtaatgc gcgtgtagcc caattccgac
     7201 tcggcggtat tgatggaaat tttgctctgt ctgattagag ccttcagctc ggcaacattg
     7261 gctttggcgg cggcaagcgc atcctgcgcg ctttccaaat cttctttaga ggtcgcatca
     7321 tccttccaca acgccgcctg acgcttatat ttcttctccg cgctgcccaa tgcaatctgt
     7381 gccgacacca gcttcgcctg atacgtttcc aacttggatt tttccgtatt gagcgtattg
     7441 gtctgcgagg tcgaattgat ttccgcaatc aaatcgccct ttttaacctg ttgcccgagt
     7501 ttgacataaa gtttcttaat ctgccccgat gcctgcgcgc cgaccgatac caggttggac
     7561 ggcgaaatct cccctgttgc agaaaccgtc cggctgatgt cgccgcgcct gaccgtttcc
     7621 gtaatataag cagcctgcgg ctcgggcttc agataagacc atccgcccca aaccgctgcc
     7681 gccgcgaccg ccgcaacagc cgcccatttc atcatttttg ccatgttttc aattctctgt
     7741 atgcgtcaat cccataaatc ggaagcggat tctaccgcaa gtccctgccc catccaaggc
     7801 ggcaaactgc cgacaataaa aataaccatt tgttttaaat ggcttataca atctgctttc
     7861 ccgaaaattt caatccgttt acatattccg ccatttccga tacggcgtta taatgtcgcc
     7921 gaccgacaaa caaatggaaa cacattgcta tgaaaaacgt tcaaaaaggc tttacgctgc
     7981 tcgagctgat gattgccgtc gccatcctcg gcatcctgac gctcatcacc tacccttcct
     8041 ataaaaccta catccggcgc gtccgcctgt cggaagtcag gacgaccctg ctgcacaacg
     8101 cgcagaccat ggaacgttac taccgccaaa aagggacgtt tgaaaactac gataaaaaca
     8161 aactgaaaca aaacaaatat ttcaacatta ccttaagcaa agtcagcccc gaccacttca
     8221 cccttcaggc cgcccccgat ccgatgacca acgaaggcga aacctgcatc gttacgctca
     8281 acgacggcgg cacactgtcc gccgccggca gcgggcagga ctgcccggac ttcgactaat
     8341 cctccgccga agcccgcaaa cccgtaaaga tgccgtccgg acacagcttc agacggcatt
     8401 tttacgggtt tgccgtccgg tttttcaaac ttgaaacctg ccgcccctca tccggcaacc
     8461 gccccgatgc cgtgcctttc caagatgtta taatataaca tataatctat atttttcaac
     8521 aacataccac ttgacctgtg agatgcggaa gcgtatgctt acccttatat cggacaacat
     8581 tccgtttaca ggtttttttg ccgtaactaa ggagcgcaat catgaaaatg caggcagttg
     8641 ttgtgaataa aaatgtagcg ggcgatgtgg aaatagtcga acgcgaggtt cgcccgttgg
     8701 aatacggcga ggcgttggtc gaagtcgaat attgcggcgt gtgccacacc gacctgcacg
     8761 ttgcggcagg cgactacggc gaaaaaccgg gccgcgtgct gggacacgag ggcatcggtt
     8821 tggttaaaga agttgccgac ggtgtgaaaa atctgaaagt cggcgaccgc gtcagcatcg
     8881 cctggctgtt ccaaagctgc ggctcttgcg aatactgcaa taccggccgc gaaaccctgt
     8941 gccgttccgt attgaacgcg ggctacaccg ccgacggcgg tatggcgacc cactgcatcg
     9001 tcaatgcgga ttatgcggtc aaagtccctg aaggtttgga tcctgcgcaa gcttccagca
     9061 ttacttgtgc cggcgtgacc acttataaag ccattaaagt ttccagtgtt cgtccgggac
     9121 agtggattgc catctacggc gcgggcggtt tgggcaactt gggggtccaa tacgcgaaaa
     9181 aagtattcgg cgcgcacgtt gtcgccatcg acatcaacga cgacaaactg gcgtttgcca
     9241 aagaaaccgg cgcggatttg gttgtcaacg ccgccaaaga agacgctgcc aaagtgattc
     9301 aggaaaaaac cggcggcgca cacgctgcgg tcgtaaccgc cgtatctgct gccgcattca
     9361 actctgccgt gaattgcgtc cgcgcaggcg gacgtgtggt tgccatcggg ctgccgccgg
     9421 aatcgatgga tttgtccatc ccgcgtttgg ttttggacgg catcgaagtg gtcggctctt
     9481 tggtcggcac gcgcaaagat ttggaagaag ccttccaatt cggcgcggaa ggtttggttg
     9541 tgccgaaagt ccaactgcgt gctttggatg aagcacccgc cattttccaa gaaatgcgcg
     9601 aaggcaaaat caccggccgt atggtgatcg atatgaaaaa agaatgcagc tgcggtcatc
     9661 accactgatt tgacgtggcg atacacatcg aaatgccgtc tgaacgctgt tcagacggca
     9721 ttttttatgg atttggattt gattttaatc cgttctgttt gaaatgccgt ctgaaaaccc
     9781 gtatcgcaac acgtcattaa aaacggcaag attcagccgt tctgcaaccc gttcaaacgg
     9841 attccgccat ttccaacgcc tgtttgatgt ccacggcgac gacgcgcgat acgccttttt
     9901 cctgcatcgt tacgccgacg agctgctctg ccatttccat ggtcaggcgg ttgtgggaga
     9961 tgtagaggaa ctgggtttgc gccgacattt ctttgaccag gttacagaaa cgcgaagtgt
    10021 tggcgtcgtc tagcggagcg tcgacttcgt ccaaaagaca gaacggagcg gggttgaggc
    10081 tgaacagggc gaacacgagg ctcatcgcgg taagggcttt ttcgccgccg gaaaggaggt
    10141 ggatggtgct gtttttcttg ccgggcggac gcgccataat ggacacgccg gcggtcaaaa
    10201 ggtcgtcgcc tatcattttg agggtggctt cgccgccgcc gaacagggtc gggaagaagg
    10261 tttggacttt gccgttgacg gcatcgaagg tttctttgaa acgcgctttg gttttgtcgt
    10321 cgatttgggc gatggcttct tccaaaaggg tgatggctgc ctgcacgtct tcgctttggc
    10381 tgcggtagta gccgtcgcgt tcgcgcgctt cttcgagttc ttgcagggcg gcgaggttga
    10441 ccgcgccgag ggcttcgatt tgttgcgaga ggctgccgat gctgctgttc aatactttcg
    10501 gcgattcttg cgtcaacgct tcgagcgcat ccaaatcggc ggcgcgttcg gtcaggtttt
    10561 gatggtagcg tttggcgttg atcagggctt cctgttgttg caacagggcg gtttgggtgg
    10621 cggcctgaag ctgcggcagc ttggtttgca gggtttgtac gcgggcgtat tgttccctac
    10681 cctgctcctg aatctgcgcg agtttctcct gtacgacaat atattcttcg tccaaactct
    10741 gtacggcttc gctcaactct tcgagcttga tgtgctgctc gtcgttttgg aactcggttt
    10801 cataggcgag ggcaagctct tgctggcgtt cctgccaatc gaaggtttgt tgttcaagct
    10861 gggcaatttg ctgttggtag ttttgttttt gctggttgag cttgtggacg gcgacttcgg
    10921 caagcccgta ttggcggttg gcttccaaca gggcaagctg cgcctgtttc agacgacctt
    10981 gctgctcttg gcggctgtgc gcggcggttt gctgctggtg ttcgagttcg gcggcggctt
    11041 cctgcaaagt aaagatgtcg tctgaaagcc cgtcggacgt gtgttgcaac acggtctgct
    11101 cttccgccaa ctgcgccagt tcgcgctcga tgtgttcgcg gcggatttgc ccttggttgg
    11161 tgcgcgccag aagttcggcg gcgcgttgct gcgcctgact gtattggcgc gtgtgctgct
    11221 gttgttgctg catcaggttt ttgtgctgca cttcggacga gcgcacggcg gcttccgcct
    11281 gtttgaacgc ggcttcggcg gcggagagtt cgggggctag gttgtccagt tcggatgcga
    11341 tgccgtcgag gtgcgctttt tgggcaatca ggctttcctg cgcgggcttg gcatagagca
    11401 ggacgctgac cttatcgacc tgatgacctt cgggcgtgag ccagatttgg tgtgcgccca
    11461 aatcgttttg atgcgcgagg gcatagctca aatcgggcgc gcacaatacg ccgtcgagcc
    11521 agtggtgcaa tgccgtctga aacggcggct gcgcttggat ttggttcagc aatgcctgta
    11581 cgggcaggga ttttttgatg ccgccggaga gaccgtctga aagccacgcc gcctgcccct
    11641 gcggcaaagg cgcgggggga acgaaaccat gcggtacgct acgggcttgc aggcgttcgg
    11701 caaggatgac ggacaaggcg tgctgccact cggcgggcgc ggtgatgtgt tgccacagtt
    11761 gcggcgcggc ggcgtggtcg cttgcctgcc agaagtcggc ggcttcctgc tgttgcgaca
    11821 ggatttgcga caacgcctgc tgctgcgcct gcaaggtgat gtgttgctgc ttcaggcttt
    11881 ggaagcggtt tgaggccgtc tgaaacgcct cgcgggcggc gtgtaaggct tcttcggcgg
    11941 cgatgatttg ttcttcgtaa tgctcttgcc ggttttgcaa cagggcggcg gcttcctgcg
    12001 cggcggcggt ttcggcttcg tcgggcaggt tcagggcttg gttttcctgt ttcagacggc
    12061 ctttgcgctc ttcgtgcttg gcaacggttt gttcggcatg ggcaagctgc tgctgcttca
    12121 acgccaactc gcggcggatg cggtttgcct cgtcctgccg ggtttggaag gcggcgttga
    12181 gcgcggtttg ggcctcttcc aattcgggca gacgctcttc gtgttcggca acctgcatcg
    12241 cccattccgc caattcggtt tgcttttctt ccgcttgcaa ctcgttttct tcaagctgca
    12301 cgcggatttg ctgctgctct tgatgaatgc gttgcaactg cgcctgcgct gcctgcttgt
    12361 cgcgttcgat gcgttgatgc aggttttgcc ggtggcggat ttgttcttcc aaacgggcaa
    12421 tctgctcgcg caacacgccg cgtttgttgc tcaattcatg caccgcctgc tgctgcgact
    12481 gttcggcggt ctgcaaggcg tgtacttcat cgtttaacgc ctgaacctgc gcggcggttt
    12541 cgtcctgctg cgcctgcaaa gactgatgct gcgcggtcgc cttgtcggcg gcggcaagcg
    12601 attgctgcca ttgggcgtaa tcgagcaaat cctgttgttg gttcaactgc gcggtcaggg
    12661 atttgtagcg ttcggcggtt tccgcctgtt tctccagctt ttccacctga cgcgccaatt
    12721 cgttctgcaa atcgcccaaa cgctgcaaat gctcgcgcgt gtctttcaga cggccttccg
    12781 tctccttgcg gcgttcctta tatttggaca cacctgccgc ctcctcgata taggcgcgca
    12841 attcctccgg ccgcgcttcg atgatgcgcg aaatcatccc ctgctcgata acggcataac
    12901 cgcgcgcgcc cacgcccgta cccaaaaata aatcggtaat gtcgcggcgg cgcacggtct
    12961 gattattgat gaaataagtc gattcgccct gacgcgtcag ctgccgcttg atactcacct
    13021 cggcatactg cccccacgcc ccctgcaaac tgtggtcgct gttgtcaaac accagctcca
    13081 ccgaagccct cggcgcagga cggcgcgtcg ccgcaccgtt aaaaatcacg tcctgcatac
    13141 tctcgccacg aagctgcttc gccgaagcct cgcccaacac ccagcgcacc gcgtcaatca
    13201 cattcgactt gccgcagccg ttcggcccga taaccgcaac aagctgcccc ggaacatgaa
    13261 tcgtggtcgg gtcggtaaaa gatttgaagc cggagagttt gatgtgggtc aggcgcataa
    13321 tggtcggaaa aaataaaaaa gaagcgtatt ttaacggaaa tcccgccgca ccaccccata
    13381 tcttgccggc aaaaccttac cgcatcccgc acccgcgatg ccgtctgaag ccttcagacg
    13441 gcatttttaa cgcgcccgaa ccccggttcc gccaatgccc gatgcttggc gcggcatttg
    13501 ccgtattgcc ccgccgccgg gcgcgttata atccgccacg acatcctccc gatttggaaa
    13561 cctgcctatg aaacccgcct atttcatctc cgacctgcat ttgagcgaaa agcaccccga
    13621 actgaccgcg ctgttgctgc gttttttacg ttcttccgcc gccgggcagg cgcgggcggt
    13681 ttatattttg ggcgatttgt ttgatttttg ggtgggcgat gacgaagttt ccgagttgaa
    13741 tacttcgatt gtgcgggaaa tcaggaaatt gtccgacaaa ggcgttgccg tgttcttcgt
    13801 caggggcaac cgcgatttcc taatcggtca ggatttttgc cgacaggcgg gtatgacgct
    13861 gctgccggat tactcggttt tggacttgtt cggctgcaaa accctcatct gccacggcga
    13921 cactctgtgt acagatgaca gggcatacca acgtttccgc aaaatcgtgc atcggaagcg
    13981 gctgcaaaaa ctgttcctga tgctgcccct gaagtggcgc acgcgccttg ccgccaaaat
    14041 caggcgtgtc agcaaaatgg aaaaacaggt caagcccgcc gatattatgg atgtcaatgc
    14101 cgcctttacc gcgcggcagg ttcgcgcctt taacgcggaa aggctgatac acgggcatac
    14161 ccaccgcgag catatccatc acgaaaacgg ctttacccgc atcgttttgg gcgactggca
    14221 taacgactat gcttcaatcc tccgcgtaga cggtgacggc gcggtattcg tgccgctgga
    14281 aaaatactga aaatgccgtc tcaagccttt cgggcggctt tttttacacg cccctttccc
    14341 acactccctc cgccgcatca tcaccatcct taacccgcat taaggggcgt tatcgataaa
    14401 atgcttgacc gattacataa gattacgtaa agtgtgcaaa cgcataacaa ttggtcttac
    14461 caatttatct tttaaccaaa atatcgggtg tacgggattt gaacgtcttt ccaaacctcg
    14521 acgcgcctta tgatgcaaat gcctaaaaag gattcgttat ggcacttttc ctcagcatat
    14581 tccccatcgt cctgctgatt tggctgatgg tgaaaaaaaa cagtatgccc tcctacgtcg
    14641 cgctgccgat taccgcagtg ctgatttacg ccatcaaact tttctacttc gacgatgcgg
    14701 gcatgctgct caacgccacc gccgcttccg gcctcgtcaa aacgctcacg ccgattaccg
    14761 tgattttcgg cgcgattatg ttcaaccgta tgatggaaac cacgggctgc atcgatgtca
    14821 tccgcaaatg gctggcgacc atcagcccca accccgtagc gcaactgatg attatcggct
    14881 gggcttttgc ctttatgatt gaaggcgcat ccggcttcgg cacgcctgcc gcgattgccg
    14941 cgccgattct gatgagcttg ggcttcaacc cgttgaaagt ggcgattttc actttggtga
    15001 tgaactccgt ccccgtctct ttcggcgcgg tcggtacgcc gacctggttc ggtttcgcac
    15061 cgctgaacct gagtgccgaa gacatcctcg ccatcggcag gcagaccggc gtaatgcact
    15121 tcttcgcagg tttcgtcatc cccgtcatcg gcttgggctt catcgtacct tggtctgaaa
    15181 tccgcaaaaa cttgggcttc gtcgccattg ccgtcttctc ctgcaccatt ccttatgtcg
    15241 cattggcgat ggtcaacgaa gaattcccgt cgctcgtcgc cggcgcgatc ggcctgatgg
    15301 tgtccgtatt cgccgccaac caaggctggg gcttgagcaa agaccacgcc aaagacccga
    15361 atgccgaaaa agtgccgttc gcccaagtcg ccaaagcact cgcccctttg ggtatgctga
    15421 tcggcatgct ggtggttacg cgcatcaaac agctcggcat caaaggcatt ttgaccagca
    15481 aagaagaatg gttcagcttc caactgccgt ttgatttgtc caaaattacc gtcagcgact
    15541 ccctgacgat tactttcggc aatattttcg gacaagatgt cagcgcgtct taccaaacgc
    15601 tgtacgtccc ggcttggatt ccgtttgtgc tgaccgtttg gatttgcatc ctgctgtata
    15661 aaaccaaatt caaagatgcc tggacgattt atgccgtaac tttcaatcaa accaaaaaac
    15721 cgctgcttgc cctgatgggc gcgctgatta tggttcagct gatgctggtc ggcggcgaca
    15781 attcgatggt gaaaatcatc ggtaaggaat ttgccgcaat ggcgggcgaa cactgggttt
    15841 atttctcgcc gtatctgggc gcgatcggtg cgttcttctc cggctccaac accgtgtcca
    15901 acctgacctt cggaccgatt cagcagcaaa tcgccttgga taccggcctg tccgttaccc
    15961 tgattctggc gttgcagtcc gtcggcggcg cgatgggcaa tatggtgtgc ctcaacaaca
    16021 tcatcgccgt atgtaccgta ttggacgtga aaaattccga aggtgcgatt atcaagaaaa
    16081 ccgttatccc gatggcgatt tacggcgtga ttgccgtcgt cgcggcaatg attttcttcc
    16141 tctaggcgaa acgccaccat ccatagcggc agcacgcggg gaacggaaac cgccgttccc
    16201 cgtccgggcg gcgcgtcggg cagaatcccg gtgccggaac aagcccgccc cgccaaacaa
    16261 atgccgtctg aaaccggaaa aggcttcaga cggcattttt ccgccgcttg cgttcaggcg
    16321 taatgatgat gcggcatctt gccgtcaagg cggtagcgcg tgccgcaata cgggcaggca
    16381 acgctgcccg attctccttc gcacaaaggc aaaaacactc tcggatgccc gttccactgc
    16441 tcgttgtcgg gtcccgagca atacagcggc agattttccg gcaacacgga aatttcctgc
    16501 agattcagat tgtccatttg attttccttt gcgtggttgg cgtgcgccca ttttacgcca
    16561 tcggcaggct aaaggatatt ttcggcgcaa agccgcaatc cgctataatc cccacttttc
    16621 agacggcata ccatgactgc gcttaccctt ccggaagaca tccgccaaca agagccatcc
    16681 gccctgctct ataccctcgt ttccgcctac ctcgaacaca ccgcccaaac cggcgacgaa
    16741 tccctctcct gcctgtccga cgaccagcac acgctgaccg cattctgcta cctcgacagc
    16801 caagtcgaag aaggcggctt cgtgcaactc atcgcctccg gttacggcga atatattttc
    16861 cgcaacccgc ttgccgacag cctgcgccgc tggaaaatca aagccgtgcc gaaagtcttg
    16921 gacaaagcca aagccctcta cgaacaacac ggcaaaacca tcgaaacgct cgccgacgga
    16981 ggcgcagaca tcgaatccct gcgcaaacag ttccccgact ttgaagaatg ggacggcgca
    17041 tactacgaag ccgccgaaca agacctgccc ctgcttgcag aacacataca gtcaaactgg
    17101 gaaaccttcg cccatatcgg gcaggcgtga ttgcgtctgt ttccagcccg tgtaaaacag
    17161 cgtaaaatcg gcaaacccga atgaccttcc gtccccatca aggagcaagc tatgttcttc
    17221 aagcacatcg aagccgcccc cgccgatccg attctcggtt tgggcgaagc gttcaaagcc
    17281 gaaacccgcc ccgaaaaagt caacctcggc atcggcgttt ataaagacgc atccggcgcg
    17341 acacccctcg tcaaagccgt caaagaagcc gaaaaacgcc tgttggaaag cgaaaccacc
    17401 aaaaactacc tcaccatcga cggcgttgcc gactacaacg cgcaaaccca aatcctgctg
    17461 ttcggcaaag accacgaaat catcgccagc cgtcgcgcca aaacagcgca aagcctcggc
    17521 ggtacgggcg cattgcgtat tgcggccgag tttgccaaac gccagttgaa cgcgcaaacc
    17581 atctggattt ccaatccgac ttggcccaac cacaacgcca tcgccaaagc ggtcggtatc
    17641 caagacaaac cttatcgtta ctatgatgcc gccaaacacg gtttggattg ggacggcatg
    17701 attgaggact tgagccaagc gcaaaaaggc gacatcgtcc tgctgcacgg ctgctgccac
    17761 aatcctaccg gcatcgaccc tacgcccgaa caatgggaaa ctttggcaaa actttctgcc
    17821 gaaaaaggct ggttgccgct gtttgacttt gcctaccaag gcttcggcaa tggtttggaa
    17881 gaagatgcct acggcttgcg cgtgttcttg aaacacaata cagaattgct gattgccagc
    17941 tcttattcca aaaacttcgg tatgtacaac gagcgcgtcg gcgcgttcac tttggtggcc
    18001 gaagatgaag aaacagcagc ccgcgcccac agccaagtca aaaccatcat ccgtaccttg
    18061 tattccaacc cggcttcaca cggtgcgaac accattgcgc tggtgttgaa aaatgatgat
    18121 ttgaaagcac aatggattgc cgaactcgat gaaatgcgcg gccgcatcaa agccatgcgc
    18181 caaaaatttg tcgagttgct caaagccaaa ggtgcaagcc aagactttga tttcattatc
    18241 gaacaaaacg gtatgttctc tttcagcggt ctgacccccg aacaagttga ccgtttgaaa
    18301 aacgagtttg ccatttatgc cgtccgctcc ggccgcatca acgtcgccgg cattaccgac
    18361 gacaacatcg attatctgtg cgaaagcatc gtgaaagtct tgtaaaacaa aagcgcatac
    18421 gaaatgccgt ctgaacctta accagttcag acggcatctg ttttttgtta aaaaacaata
    18481 aaccataaca atttcaaaca agctaaaaaa catctataaa atcaacccaa tatgatgaac
    18541 tcaaataaaa aatacgaata tcatcattct caaattcaaa atttgaacgc cacagtatta
    18601 tccgaagcca caaaaacctt tccgccgtca ttcccgcgca ggcgggaatc cgggaaccca
    18661 atgctgcaag aattgattag aaacaactga aaccgagcaa actggattcc cgcctgcgcg
    18721 ggaatgacga aataaggcga gtaaaactga tgaaacaaat tgattaaaaa caataactta
    18781 tattatttta tttaaatttt cgataaaatc aattttccgt attcaatact gctccaatca
    18841 attcccgcgc accgtcgtcc gccaatttct tagcgacagc gcgtccgagc gcgtcggcat
    18901 attcggcagg ggcttgcgcg tccgcccgca acacaaccga accgtcggga tgaccgacca
    18961 agccgcgcaa ggtcagcaag ccgttttctt ccgtgcaata tgccgccagc ggcacttggc
    19021 agcttccgcc caaagcgcgc gccaacgcgc gttcagccgt aacgcaggcg tgtgtggtat
    19081 cgtggttcaa cggctttaag acttcataca aatcttcgcg atgcgctgca atttcaatac
    19141 ccaatgcacc ttgtccggcg gcaggcagac tgtcagattc cgacaaaatc atgcggatgc
    19201 gttcgtccaa ttccagacgc tgcaaaccgg cggcggcaag gataattgcg tcgtattcgc
    19261 cgttatcgag tttggacaaa cgggtttgca cattgccgcg caaaggtttg ataaccaaat
    19321 gcggatagcg cgcacgcaac tgggcttcgc ggcgcaggct ggatgtaccg acgacagcgc
    19381 ctttgggcat ttcttccaaa cgcgcgtatt ggttggacac aaacgcgtca aacggattgg
    19441 cgcgttcgcc gatggcggca agcgcgaaac cttcaggcaa atccatcggc acgtccttaa
    19501 tcgaatgcac cgccaaatcg gcgcgcccgt catataaagc ctgttccaac tctttgacaa
    19561 acaagccttt accgccgact tttgacaaag ttttgtccaa aatctgatcg ccgcgcgtgg
    19621 tcatgcccaa aatctcgact tcgcaatcgg gatacagagc cttcagacgg ccttggatat
    19681 gctttgcctg ccacatagca agcaggcttt cgcggctggc gatgacgagt tttttcgggt
    19741 tcatgttcaa tgcgttcaaa gaatggaaac ggcgtgaagt ctatcatatt ttgaacgttt
    19801 cttcagacgg caccgtcagg tctatgttat atttacaccc acaaaacacg aaacccgcca
    19861 caatgaaacg caccaaaaaa taccctttct taacgttgca gcggcaacgt ttccatttga
    19921 actttgaaaa cgcctcttcc gccgccggca tcccagccga acgcgatttc taccgctggg
    19981 cgtggtctgc cttgaaaagc ggataccgcc gcgccgacat cagcctgatt cttctggacg
    20041 aagaagaagc ccgagcctac aaccgcgact accgcggcaa agattacgcc accaatgtat
    20101 tgagtttcgc gctcaacgaa ggcgaaatcc tgccctgcca agtttcggaa aaactgtacg
    20161 gcgatttgat tatctgcccg caagtggttt tgaaagaagc cgccgaacag ggcaaaacgc
    20221 ccgaacggca ttttgcccac ctgaccatac acggcgtttt gcacctgatg ggctacgacc
    20281 acatcgaaga cgatgaagcc gaaataatgg aagccgaaga aatccgcctg atgctggcgg
    20341 caggcttccc caacccctac caagaggacg aatattaaaa tggacggtac gcagtcgaaa
    20401 cccaagtttt tcgaacgcct gatctcccga cttgccggcg agcccgactc cgccgaagac
    20461 gtattgaccc tgctgcgcca agcgcacgag caggaagttt ttgatgcgga tacgctttta
    20521 aggttggaaa aagtcctcga tttttccgat ttggaagtgc gcgatgccat gattacgcgc
    20581 agccgtatga acgttttaaa agaaaacgac agcatcgaac gcatcaccgc ctacgttatc
    20641 gataccgccc attcgcgctt ccccgtcatc ggtgaagaca aagacgaagt tttgggtatt
    20701 ttgcacgcca aagacctgct caaatatatg ttcaaccccg agcagttcca cctcaaatcg
    20761 atattgcgcc ctgccgtctt cgtccccgaa ggcaaatcgc tgaccgccct tttaaaagag
    20821 ttccgcgaac agcgcaacca tatggcgatc gtcatcgacg aatacggcgg cacgtcgggt
    20881 ttggtaactt ttgaagacat catcgagcaa atcgtcggcg acatcgaaga tgagtttgac
    20941 gaagacgaaa gcgcggacaa catccacgcc gtttcttccg aacgctggcg catccacgcg
    21001 gctaccgaaa tcgaagacat caacaccttc ttcggtacgg aatacagcag cgaagaagcc
    21061 gacaccattg gcggcctggt cattcaagag ttgggacatc tgcccgtgcg cggcgaaaaa
    21121 gtcattatcg gcagcctgca atttaccgtc gcacgcgccg acaaccgccg cctgcatacg
    21181 ctgatggcga cccgcgtgaa gtaagcagag cctgcccgca ccgccgtttc tgcacagttt
    21241 aggatgacgg tacggacgtt ttctgtttca atcctcccca tccgccaaac ataaaaatgc
    21301 cgtctgaaac cggaaaacag gttcagacgg catttcgctt atccgcttat tttttgctca
    21361 aagtcagccc cgtccagccg aataacaggg ttaaagccaa actcaaatag cagaaaaagg
    21421 catagggcag atattcccag accggcacac ccagcgcatg gctgataaat acgccgcaca
    21481 cgctccacgg aacaagcggg ttgatgaccg tacccgcgtc ttcgagcgta cgcgagaggt
    21541 tgcgggaatg tagtccgagc ttatcgtaaa cgggtttgaa cgtttcaccc gacaacaaaa
    21601 tactcaaata ttgctcgccg atcaggaaat taaccccgac cgaagtcatg gcaacgctga
    21661 atgtggcacg tccggcattc gtcaaaaagc tgcggacggc atccagcagg gaaggaatcg
    21721 cgcccagcgc aaacagcagc ccgcccaaac tcatcccgag aatcacgatg gtttgcgtga
    21781 aaaacatact ttccaagccg ccgcgcgaaa tgagtttggc gatgtcttta aacgcttcgc
    21841 cttcgagttt gtaaccgccg taaaaccacg cgccgagctg acgcagatcg ggcgtgctgt
    21901 gcagatacgt tacggcaacg gcaaccatga cggtaaagag catggcgacc acggcgttga
    21961 cgcgcatcaa tgccaaaatg accaacagcg caaacggaat cagcgaatag ccgtgcacca
    22021 atcccgtggc ttcaagctgg ctgcggaagg attcgacgct gttcaaatcc tgcgcggcga
    22081 cactgggaag aagccacagc atcagtgccg cactgatgag ccacgcggga atggtggtgt
    22141 acatcatatt tttgatgtgc tcaaacaggt cgatgccgac gatggacgcg gaaatgcccg
    22201 tggtgtcgga aagcggggac attttgtcgc cgaaaaacgc accggaaaca atcgcgcccg
    22261 ccgtcatcgc catatcggca tgaaacgccg ccgccatccc cataaaggca acgccgacgg
    22321 tggcgcaggt ggtcaggctg ctgccgatgg atacgccgat gacggaacac agcgcgaaag
    22381 cggaaaaata aaaataagtc ggggaaatca gcccgaaacc gtaatacatc agcgtcggaa
    22441 tcgcaccgct catcatcagc gcgctgacca tcagcccgat gaagaaaaac aggtaaatcg
    22501 cgcccatacc ctgattcaac gcgcctatca tgccctgctg catatcgttg tatttcaaac
    22561 cgcgcgccaa gccgtacaaa atcagcacga cgatggcggc aataatggac atatgcggca
    22621 gccactccaa tgaaatgatg gtatagccca tcgcggcaat cagagcgacg acgacggcaa
    22681 gtgcctcacc gcgcggcata tcgagtaagg atttgaaagc gaacatgatt ctttgcccga
    22741 tttgatataa agttaacgga ttctaacaca ctataccgaa tccgcccgac gttttcagac
    22801 ggcattttag cgttttacaa aagccggaca gaaacaaaac ggcaattttc tcaatttaaa
    22861 ttaatttttt tgaaatataa ttttttattt ttttgtttgt tgacatataa gacttttatg
    22921 gattgaagta ttaaaacctc ggcacacacg ccgcaaacac cggtacgcaa aaatgccgtc
    22981 tgaaagatgt tcagacgaca tttctcaaga aaaagattat ttgtccgccc tatatccgta
    23041 caccgcaaaa aacacgatat acagataaca gatgacggaa acgacaaacg aagacatcag
    23101 ggtgtaagta tctgccaccc agccctgcac gacaggaacg accgcaccgc cgacaatcgc
    23161 ggtacacagt acaccggaag ccgcgttggt aaattttccc aatcctttgg ttgccaaaga
    23221 gaaaatcgtc gggaacataa tcgagttgaa aaaaccgatg gcaagcagcg accacatcgc
    23281 cacatccgca ttgccgctac ccgtcgccat cgcgacggca agcagtacga ccgcagccga
    23341 tgcgttaaac gccaaataac ggttgggcgc gaatttcgcc atcaccgccg aaccgaggaa
    23401 acgtccgacc atcgcgccgc cccaatagaa cgacaggtaa tgcgcggcag aagcatgatc
    23461 cagccctttc agataaccca atacgttgac catcaacgaa ccgatagaca cctccgcgcc
    23521 gacatagcag aaaatgcctg ccgtgccgaa cacgagatgt ttgtattgcc atacgctggt
    23581 tttgccgtcg tggttgtgcg cgctttcctc ggcggcaatt ttgcgcgcgt cgggcagccg
    23641 gatcattttc acgaaaacgg caaggataat cagcagcccc gccagtccca aatagggaat
    23701 ctgtacggaa gaaatctgtt ccgccttgct gacggtttgg gttgcgtccg ccagaatcag
    23761 gaacgcgccg atttgcggcg caatggtcgt acccaaagcg ttaaacgcct gaaccagcgt
    23821 cagtgttgcc gattccttgc cgggtttcgc cagcagggta acataaggat taccggcgac
    23881 ctgaagcagc gttacgccgg aagccaaaat aaacaacgcg cccaaaaata ccgcgtaaga
    23941 atggctaccc gcagcaggat aaaacagcag gcatccgacc gccgtcagca gaaagccgcc
    24001 gataacgccg tttttgtagc cgactttgcc gacaaaagcc cccatcggga tggacatcac
    24061 cgcataggcg gtaaagaaac agaattggat cagcatcgcc tgaacgtaag acaggtcgaa
    24121 aatttctttc aaatgaggga taaggatgtc gttcatgcag gtaataaaac ccatcatgaa
    24181 gaacagcgtg gtcaagacga ccaatgggga cgtatgattg ttttgtgatt gcgcagacat
    24241 gatttgcctt tcgtggtgtc ggaatattac ccgtttcaag aaaatagaaa ttgacatttc
    24301 ttaacggcaa tgcaaatcat actgatattt gaacattttg caaactttat cggatgcagg
    24361 caatgcgccg atctgaaatt caagcagcag aaattaatat tttacagttg cttgcataga
    24421 ttttaataga ttttaaaaaa ttccggcgga cttgcaaaaa ccgttcccaa taaaatgaaa
    24481 atccgccgcc gtatccggaa aattcggaaa tgccgcatcg tggcatcaaa accggagcgg
    24541 aaagaatgtc gtatgcccga caaaaaaggg gcatacggct gtgccgtatt ttttgcaccg
    24601 tcctccgccc ttcggcgggg gcgtgaaaat atagtggatt aacaaaaatc aggacaaggc
    24661 gacg
//
LOCUS       DAXHSF010000013        23466 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.013, whole genome shotgun sequence.
ACCESSION   DAXHSF010000013 DAXHSF010000000
VERSION     DAXHSF010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23466
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.013"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(214..1179)
                     /locus_tag="ACFM8S_000417"
     CDS             complement(214..1179)
                     /locus_tag="ACFM8S_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6313541.1"
                     /translation="MKPRFYWAACAVLLTACSPEPAAEKTVSAASASAATLTVPTARG
                     DAVVPKNPERVAVYDWAALDTLTELGVNVGATTAPVRVDYLQPAFDKAATVGTLFEPD
                     YEALHRYNPQLVITGGPGAEAYEQLAKNATTIDLTVDNGNIRTSGEKQMETLARIFGK
                     EARSAELKAQIDALFAQMREAAKGKGRGLVLSVTGNKVSAFGTQSRLASWIHGDIGLP
                     PVDESLRNEGHGQPVSFEYIKEKNPDWIFIIDRTAAIGQEGPAAVEVLDNALVRGTNA
                     WKRKQIIVMPAANYIVAGGSRQLIQAAEQLKAAFEKAEPVAAGKE"
     gene            1415..2371
                     /locus_tag="ACFM8S_000418"
     CDS             1415..2371
                     /locus_tag="ACFM8S_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC6313542.1"
                     /translation="MNTAAIYRQYQTYVRSDKSGWALDGCSDSALIAQAKQPGLHLEM
                     CINRFDSGITLSRMRGGGTGAFPTEIHNFSHNCALFVMVSGQNRLQMGGREYRPSAGE
                     IWLVRGELADVSETLLPDNSGMCALHLDFSLEKLRRWHDEGLLDERLFSPQTIGRFAL
                     QRLAQNAAALTAAACPLLQRPFESDGFGLLADEAAALELSARLLRFTFRRHDNGYRRR
                     RIDEAADILQHEFARPLTIAEIARRVGLNECYLKRYFKAQTGETVAGCLRRLRLEHAL
                     ALIESGSTVQAAMHFCGYRHAGRFNEAFRRHYGFLPSDVKKC"
     gene            complement(2485..4500)
                     /locus_tag="ACFM8S_000419"
     CDS             complement(2485..4500)
                     /locus_tag="ACFM8S_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family protein"
                     /protein_id="HFC6313543.1"
                     /translation="MKSYPDPYRHFEDLDSAETQNFAAEANAETRARFLNNDKARALS
                     DGILAQLQDTRQIPFCQEHRARMYHFHQDAEYPKGVYRVCTAATYRSGYPEWKILFSV
                     ADFDELLGDDVYLGGVSHLVEQPNRALLTLSKSGGDTAYTLEVDLEAGELVEGGFHFP
                     AGKNYVSWRDENSVWVCPAWDERQLTESGYPREVWLVERGKSFEESLPVYQIGEDGMM
                     VNAWRYLDPQGSPIDLIEASDGFYTKTYLQVSAEGEAKPLNLPADCDVVGYLAGHLLL
                     TLRKDWHRANQSYPSGALVAVKLNRGELGAAQLLFAPDETQALESVETTKRFVVASLL
                     ENVQGRLKAWRFTDGKWQETELPRLPSGALEMTDQPWGGDVVYLAASDFTTPLTLFAL
                     DLNVMELTVMRRQPQQFVSDGIEVRQFWAMSSDGERIPYFHVGKNAAPDTPTLVYAYG
                     GFGIPELPHYLGSIGKYWLEEGNAFVLANIRGGGEFGPRWHQAAQGISKHKSVDDLLA
                     VVRDLSERGISSPEHIGLQGGSNGGLITAAAFVREPQSIGALVCEVPLTDMFRYPLLS
                     AGSSWTDEYGNPQKYEVCKRRLGELSPYHNLSDGIDYPPALITTSLSDDRVHPAHALK
                     FYAKLRETSPQSWLYSPDGGGHTGNGTQREAADELACVLLFLKEFLG"
     gene            complement(4596..5906)
                     /gene="argA"
                     /locus_tag="ACFM8S_000420"
     CDS             complement(4596..5906)
                     /gene="argA"
                     /locus_tag="ACFM8S_000420"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFC6313544.1"
                     /translation="MSAPDLFVAHFREAVPYIRQMRGKTLVAGIDDRLLEGDTLNKLA
                     ADIGLLSQLGIRLVLIHGARHFLDRHAAAQGRTPHYCRGLRVTDETSLEQAQQFAGTV
                     RSRFEAALCGSVSGFARAPSVPLVSGNFLTARPIGVIDGTDMEYAGVIRKTDTAALRF
                     QLDAGNIVWLPPLGHSYGGKTFYLDMLQTAASVAVSLQAEKLVYLTLSDGISRPDGTL
                     AETLSAQEAQSLAEHAGGETRRLISSTVAALEGGVHRVQILNGAADGSLLQELFTRNG
                     IGTSIAKEAFVSIRQAHSGDIPHIAALIRPLEEQGILLHRSREYLENHISEFSILEHD
                     GNLYGCAALKTFAEADYGEIACLAVSPQAQDGGYGERLLAHIIDKARGIGISRLFALS
                     TNTGEWFAERGFQTASEDELPETRRKDYRSNGRNSHILVRRLHR"
     gene            complement(5903..6337)
                     /locus_tag="ACFM8S_000421"
     CDS             complement(5903..6337)
                     /locus_tag="ACFM8S_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MJ0042-type zinc finger domain-containing
                     protein"
                     /protein_id="HFC6313545.1"
                     /translation="MPACFCPHCKTRLWVKETQLNVAQGFVVCQKCEGLFKAKDHLAS
                     TKEPIFNDLPEAVSDVKLVHRIGTRAIGKKQISRDEIAGILNGGTTQPDIPPATAATP
                     AAAPQVTVPPAAPARQDGFNWTIATLFALIVLIMQLSYLVIL"
     gene            complement(6411..7052)
                     /gene="pyrE"
                     /locus_tag="ACFM8S_000422"
     CDS             complement(6411..7052)
                     /gene="pyrE"
                     /locus_tag="ACFM8S_000422"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFC6313546.1"
                     /translation="MTDFRQDFLKFSLAQNVLKFGEFTTKAGRRSPYFFNAGLFNDGL
                     STLQLAKFYAQSIIESGIRFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAK
                     DHGEGGVLVGAPLKGRVLIIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGT
                     GELSAVQEVEKQYGLPVAPIASLNDLFILLQNNPEFGQFLEPVRAYRRQYGVE"
     gene            complement(7114..7851)
                     /locus_tag="ACFM8S_000423"
     CDS             complement(7114..7851)
                     /locus_tag="ACFM8S_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HFC6313547.1"
                     /translation="MLSARYLHLHEALGLGPMWLKQAAAVLPPKNTPATPAQARPQTV
                     RAAPIRPSQPHNGQARLETMKALETAAVPTRKPAPETETPLPGLSDGIAPVPAASGIT
                     KLAVVSLCPPIEDAVYGQLFHGKAGVLLDNILKAVGLDAAYVHKTSWVKTAAVGNPMP
                     SEQAVANALGQIARELDGCRAPAVLFLGQAFVQPERQTMIETLCGSRPFFIIDHPARL
                     LRQPELKARAWQVLKQLKRALRQGGGS"
     gene            complement(7845..8285)
                     /gene="rimI"
                     /locus_tag="ACFM8S_000424"
     CDS             complement(7845..8285)
                     /gene="rimI"
                     /locus_tag="ACFM8S_000424"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFC6313548.1"
                     /translation="MNIRRASPSDCAALAVLDAVCNPSAWTQRQFESALVSPSEEVFL
                     AEKDGGIAAFIVWQNLPDESELHLIATAPECRRQGIASALLEYWYAHLPEGTQRLLLE
                     VRAGNTAAQALYAAHGFSIAGRRKNYYRTADGKTEDAVLMEKIC"
     gene            complement(8282..8959)
                     /gene="tsaB"
                     /locus_tag="ACFM8S_000425"
     CDS             complement(8282..8959)
                     /gene="tsaB"
                     /locus_tag="ACFM8S_000425"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFC6313549.1"
                     /translation="MQADFNRPVLAIDTGTSYLSLALRADGETHLFHQEAGSRQSELI
                     LPEIRTLFRNAGITAADLGAIVYAKGPGAFTGLRIGIGVAQGLATPFDTPLIGIPTLD
                     AAASLPPPQSCILAAADARMGEVFYAWFDTLNRRRLSDYQVGRAADIRLPEGCAFSDG
                     IGSAFALEETPPFSGKPDMPTAADFLNLAAEGGFPAVAAAHAELLYVRDKIALTAKEQ
                     AERKART"
     gene            complement(9099..10061)
                     /locus_tag="ACFM8S_000426"
     CDS             complement(9099..10061)
                     /locus_tag="ACFM8S_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="factor H-binding protein"
                     /protein_id="HFC6313550.1"
                     /translation="MPSEPLFGRHLIFASLTCLIDAVCKKRYHNQNVYILSILRMTRS
                     KPVNRTAFCCFSLTAALILTACSSGGGGVAADIGAGLADALTAPLDHKDKGLQSLTLD
                     QSVRKNEKLKLAAQGAEKTYGNGDSLNTGKLKNDKVSRFDFIRQIEVDGKLITLESGE
                     FQVYKQSHSALTALQTEQVQDSEDSGKMVAKRQFRIGDIAGEHTSFDKLPKGGSATYR
                     GTAFGSDDAGGKLTYTIDFAAKQGHGKIEHLKSPELNVELATAYIKPDEKRHAVISGS
                     VLYNQDEKGSYSLGIFGGQAQEVAGSAEVETANGIHHIGLAAKQ"
     gene            complement(10081..11145)
                     /gene="fba"
                     /locus_tag="ACFM8S_000427"
     CDS             complement(10081..11145)
                     /gene="fba"
                     /locus_tag="ACFM8S_000427"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFC6313551.1"
                     /translation="MALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIMEAADQVNAP
                     VIVQASAGARKYAGAPFLRHLILAAVEEFPHIPVVMHQDHGASPDVCQRSIQLGFSSV
                     MMDGSLMEDGKTPSSYEYNVNATRTVVNFSHACGVSVEGEIGVLGNLETGEAGEEDGV
                     GAVGKLSHDQMLTSVEDAVRFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRIDRIK
                     EIHQALPNTHIVMHGSSSVPQEWLKVINEYGGNIGETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRLASTGAVRRYLAENPSDFDPRKYLSKTIEAMKQICLDRYLAFGCEGQAGKIKP
                     VSLEKMANRYAKGELNQIVK"
     gene            11339..12256
                     /gene="xerC"
                     /locus_tag="ACFM8S_000428"
     CDS             11339..12256
                     /gene="xerC"
                     /locus_tag="ACFM8S_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFC6313552.1"
                     /translation="MVLDGFAAHFDAYLENIVREGKSEHTVAAYRRDLEELFALLAQM
                     PSEAEGGVPQGLSQRDFTAALRRLSQRGLNARTLARKLSSWRQYCAWLVKRGLMRADP
                     TADIKPPKQPERVPKALPQEWLNRMLDLPVDGGDPLAVRDHALFELMYGSGLRVSEIH
                     GLNADDVYLDEAWVHVTGKGRKQRQVPLTGKSVEALKNYLPLRQTASDGKALFTGRNG
                     TRLSQRQIQKRLAQWAAQNGDGRHVSPHMMRHSYAGHLLQASRDIRAVQELLGHSSLS
                     TTQIYTKLDFDHIARLYDEAHPRAKRQDE"
     gene            12341..14254
                     /gene="dxs"
                     /locus_tag="ACFM8S_000429"
     CDS             12341..14254
                     /gene="dxs"
                     /locus_tag="ACFM8S_000429"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFC6313553.1"
                     /translation="MNPSPLLDLIDSPQDLRRLDKKQLPRLAGELRTFLLESVGQTGG
                     HFASNLGAVELTIALHYVYDTPEDKLVWDVGHQSYPHKILTGRKNQMHTMRQYGGLAG
                     FPKRCESEYDAFGVGHSSTSIGAALGMAAADKLLGSDRRSVAIIGDGAMTAGQAFEAL
                     NCAGDMDVDLLVVLNDNEMSISPNVGALPKYLASNVVRDMHGLLSTVKAQTGKVLDKI
                     PGAMEFAQKVEHKIKTLAEEAEHAKQSLSLFENFGFRYTGPVDGHNVENLVDVLKDLR
                     SRKGPQLLHVITKKGNGYKLAENDPVKYHAVAKLPKEGAAQMPSEKEPKPVAKPTYTQ
                     VFGKWLCDRAAADERLIAITPAMREGSGLVEFEQRFPDRYFDVGIAEQHAVTFAGGLA
                     CEGMKPVVAIYSTFLQRAYDQLVHDIALQNLPVLFAVDRAGIVGADGPTHAGLYDLSF
                     LRCIPNMIVAAPSDENECRLLLSTCYQADAPAAVRYPRGTGTGAPVSDGMETVEIGKG
                     IIRREGEKTAFIAFGSMVAPALAVAEKLNATVADMRFVKPIDEELIVRLARSHDRIVT
                     LEENAEQGGAGGAVLEVLAKHGICKPVLLLGVADTVTGHGDPKKLLNDLGLSAEAVER
                     RVRAWLSDRDAAN"
     gene            complement(14339..15667)
                     /gene="miaB"
                     /locus_tag="ACFM8S_000430"
     CDS             complement(14339..15667)
                     /gene="miaB"
                     /locus_tag="ACFM8S_000430"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFC6313554.1"
                     /translation="MKKVFIRTFGCQMNEYDSDKMLAVLAEEHGGIEQVTQADEADII
                     LFNTCSVREKAQEKVFSDLGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVV
                     FGPQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
                     CSFCVVPYTRGEEFSRPLNDVLTEIANLAQQGVKEINLLGQNVNAYRGEMDDGEICDF
                     ATLLRIVHEIPGIERMRFTTSHPREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSA
                     MKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSF
                     VFIYSPRPGTPAANLPDDTPHEEKVRRLEALNEVIEAETARINQTMVGTVQRCLVEGI
                     SKKDPDQLQARTANNRVVNFTGTPDMINQMIDLEITEAYTFSLRGKVVEA"
     gene            15870..16283
                     /locus_tag="ACFM8S_000431"
     CDS             15870..16283
                     /locus_tag="ACFM8S_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HFC6313555.1"
                     /translation="MKPAVWAALLLCACTSNFGDREHQFLRDQTEEKIVQFAVRGKTT
                     RAEVEARFGRQNPFGCYAYHEVSLPIYNFLPTNFIYMKSERRHWEWCVDYDGEGVVRD
                     YRFTHKVEKDERSVIRDAVGVIRKEAGKSLSQPEK"
     gene            16347..17630
                     /gene="hemL"
                     /locus_tag="ACFM8S_000432"
     CDS             16347..17630
                     /gene="hemL"
                     /locus_tag="ACFM8S_000432"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFC6313556.1"
                     /translation="MNRNEILFDRAKAIIPGGVNSPVRAFGSVGGVPRFIKKAEGAYV
                     WDENGTRYTDYVGSWGPAIVGHAHPEVIEAVREAALGGLSFGAPTEGEIAIAEQIAKI
                     MPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
                     GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGNDIACVILEPFVGNMNLVRPTEA
                     FVKALRGLTEKHGAVLIYDEVMTGFRVALGGAQSLHGITPDLTTMGKVIGGGMPLAAF
                     GGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIRREGFYENLTALTSRLADG
                     IAAAAKAHGIEFTADSVGGMFGLYFASHTPRNYADMARSNIDAFKRFFHGMLDRGIAF
                     GPSAYEAGFVSAAHTPELINETVAVAVEVFKAMAE"
     gene            complement(17846..18118)
                     /locus_tag="ACFM8S_000433"
     CDS             complement(17846..18118)
                     /locus_tag="ACFM8S_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taxon MazF"
                     /protein_id="HFC6313557.1"
                     /translation="MPSEAQILHFRRHFYSRLKSKIYTPVIPAQAGIRKVGLVGHEVV
                     LKMKRYRKSDELKEKYSDIDETFEFIKGFIYVIALAFTAYVAIRFF"
     gene            complement(18129..18692)
                     /gene="orn"
                     /locus_tag="ACFM8S_000434"
     CDS             complement(18129..18692)
                     /gene="orn"
                     /locus_tag="ACFM8S_000434"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFC6313558.1"
                     /translation="MQDKNNLCWLDMEMTGLNPETDRIIEVAMIITDSDLNVLAQSEV
                     YAIHQSDELLDNMDEWNTATHGRTGLTQRVRESSHTEAEVEQKLLDFMSEWVPERATP
                     MCGNSIHQDRRFMVKYMPKLENYFHYRNLDVSTLKELAKRWNPPVAKSVVKRGSHKAL
                     DDILESIEEMRHYREHFLISAPRAEAQ"
     gene            complement(18710..19597)
                     /gene="prmA"
                     /locus_tag="ACFM8S_000435"
     CDS             complement(18710..19597)
                     /gene="prmA"
                     /locus_tag="ACFM8S_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFC6313559.1"
                     /translation="MPYQQITVNVNDAVAERLADALMEHGALSAAIEDAYAGTQNEQA
                     IFGEPGMPAEQIWQQSKVIALFGEHDEAAAIIQIAAQECGLKDLAYTGETIEDQDWVR
                     LTQSQFDPIRISDRLWITPSWHEAPEGTAVNLRLDPGLAFGTGSHPTTRLCLKWLDTQ
                     LKNGESVLDYGCGSGILTIAALKLGAGSAVGVDIDEQAVRAGKDNAEQNNVDAQFFLP
                     DGLPQGQFDVVVANILANPLRMLGEMLAARTKQGGRIVLSGLLDEQIEELSGIYSQWF
                     DLDPAETEEGWARLSGTKR"
     gene            complement(19715..21076)
                     /gene="accC"
                     /locus_tag="ACFM8S_000436"
     CDS             complement(19715..21076)
                     /gene="accC"
                     /locus_tag="ACFM8S_000436"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFC6313560.1"
                     /translation="MLKKVLIANRGEIALRVLRACREMGIATVAVHSEADKDSLHVKL
                     ADESVCIGPAASAQSYLNVPAIIAAAEVSCADAVHPGYGFLAENADFAEQVEQSGFTF
                     IGPKPDTIRLMGDKVSAKHAMIAAGVPCVPGSDGALPDDGEEILKIADKVGYPVIIKA
                     SGGGGGRGMRVVEKKEDLLQSVEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVIADE
                     HGNAVYLAERDCSLQRRHQKVIEEAPAPFITEEERAKIGNACADACKRIGYRGAGTFE
                     FLYEDGEFFFIEMNTRVQVEHPVTELITGVDIVQEQLRIAAGLPLQYKQKDIQVEGHA
                     FECRINAEDPYNFIPSPGPIESCHLPGGFGIRVDSHIYQGYRIPPYYDSLIGKICVHG
                     KTREQAMAKMRVALAELAVTGIKTNTPLHRDLFADAGFQKGGVSIHYLEHWLEDRKAK
                     QDK"
     gene            complement(21189..21644)
                     /gene="accB"
                     /locus_tag="ACFM8S_000437"
     CDS             complement(21189..21644)
                     /gene="accB"
                     /locus_tag="ACFM8S_000437"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFC6313561.1"
                     /translation="MDLRKLKKLIDLVEESGIAEIEVTEGEEKVRITRTIAAAPVYAA
                     PVPAAAPAVTPAAAPVAASAPAAAPAARDLSDAQKSPMVGTFYRAPGPNAAPFVEVGQ
                     QVKAGDTLCIIEAMKLMNEIEAEKSGTVKEILVENGTPVEFGEPLFIIG"
     gene            complement(21626..21883)
                     /locus_tag="ACFM8S_000438"
     CDS             complement(21626..21883)
                     /locus_tag="ACFM8S_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HFC6313562.1"
                     /translation="MRKIKLPAHFPQKPPFFDILLDIYRQLRENKHILTVVFHHRKTV
                     SEHHSARFAPLPQAGCFLKNQRNNPPEHRQPLKEQKWICAN"
     gene            22000..23040
                     /gene="queA"
                     /locus_tag="ACFM8S_000439"
     CDS             22000..23040
                     /gene="queA"
                     /locus_tag="ACFM8S_000439"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFC6313563.1"
                     /translation="MDISDFDFTLPEKLIAQHPPEVRGSSRLLVALPDMPLQDRVFGD
                     LPDYVEAGDVLVFNNTKVMKARLFGQKDSGGRIEALIERVLDNHTALAHIRSSKSPKP
                     GMGLVFEGGIRAVMVGREGELFCLRFEGGQTVYELLEQNGHLPLPPYIERAADADDDS
                     RYQTVYAKYQGAVAAPTAGLHFTEELLRRLKDKGAVTAEVTLHVGAGTFQPVRVEKIE
                     EHKMHSEWFEVPSETAAAVEAAKARGNKVWAVGTTSMRALESAARATGRLKAGQGDTD
                     IFITPGYRFNVVDRLVTNFHLPKSTLLMLVSAFSGMGHIRAAYRYAVEREYRFFSYGD
                     AMVLGRNEGVVR"
BASE COUNT         4784 a         6295 c         7035 g         5352 t
ORIGIN      
        1 ttcaacaaga gttaaaaatc gcctgaagct taaagacagc ctgcaaccca atcccctctc
       61 cctgtgggag agggctaggg agagggcggc aaaccgtagg tttgcttgag cggtattttc
      121 aacatacagg ctgcttttca attcgttgaa accgcacttt agcttcgcag aaaccccccg
      181 cttccttcgg aagctccgtt ttcagacgac tccctactct ttccccgccg caacgggttc
      241 tgccttttca aacgccgcct tcaactgctc cgccgcctga atcaactgcc gcgagccgcc
      301 cgcgacaatg tagttcgcgg caggcatgac gatgatttgc ttgcgcttcc aagcgttcgt
      361 gccgcgtacc agcgcgttat ccaatacttc gacagccgcc ggcccttcct gcccgatggc
      421 ggcggtacgg tcgatgatga aaatccaatc ggggtttttc tctttgatgt attcgaagga
      481 aacaggctgc ccgtgcccct cgttgcgtaa agattcgtct acaggcggta ggccgatgtc
      541 gccgtgtatc caacttgcca accgcgactg cgtgccgaag gcggacacct tgttgcccgt
      601 aaccgacagc accagcccgc gtcctttgcc tttggcggct tcgcgcattt gggcgaacag
      661 cgcgtcaatc tgcgccttca attccgccga gcgcgcttcc ttgccgaaaa tccgcgccaa
      721 ggtctccatc tgcttttcgc cgctggtgcg gatattgccg ttgtccaccg tcagatctat
      781 ggtggtcgcg tttttcgcca actgttcata cgcttccgcg cccggcccgc cggtaatgac
      841 aagctgagga ttgtagcggt gcagggcttc gtaatcgggc tcgaacagcg tccccaccgt
      901 tgccgccttg tcaaatgcag gctgcaaata atccacgcgc accggcgcgg tggttgcgcc
      961 cacattcacg cccaattcgg tcagtgtatc caacgccgcc cagtcgtaca cggcgacgcg
     1021 ttcgggattc ttcggcacaa cggcatcgcc ccgcgcggtc ggcacggtca gcgtggcggc
     1081 agatgcggat gcggcggata cagttttttc ggcggcaggt tcgggcgaac aggcggtcag
     1141 caggacggcg caggctgccc aataaaaacg cggtttcacg gtgtgcggtt cctttgcata
     1201 cgaataagaa tgtttattat taagcaaaca acctgcagaa accacgccgc aaaacgcaaa
     1261 attcccgata cggcaaaaca aattcccgat acggaaaagg tcgtctgaaa caccctttca
     1321 gacgaccttt tccgtttcaa ccaatccctg cgcttgatta ttggtaataa ttcctattta
     1381 attcatttgt tagacaactc gttcctatcc aatcatgaac accgccgcca tctaccgcca
     1441 gtaccaaacc tatgtccgct ccgataaatc cggctgggcg ttggacggct gttccgacag
     1501 cgcgctcatt gcgcaggcaa aacagcccgg tttgcatctg gaaatgtgca tcaaccgctt
     1561 cgattcgggc atcaccttgt cgcggatgcg cggcggcgga acgggcgcgt ttcccaccga
     1621 aatccacaat ttcagccaca actgcgcctt gttcgtcatg gtgtcggggc agaaccggtt
     1681 acaaatgggc ggcagggaat accgcccgtc tgccggcgaa atctggctgg tacgcggcga
     1741 gttggcggac gtatccgaaa ccctgctgcc cgacaacagc ggcatgtgcg cgctgcattt
     1801 ggatttttcg ctggaaaaac tgcgccgctg gcacgacgaa ggcttgctgg acgaacgcct
     1861 gttttcgccg caaacgatag gccgattcgc cctgcaacgg ctggcgcaaa acgcagcggc
     1921 actgacggcg gcggcctgcc cactgctgca acgcccgttc gaatcggacg gcttcggcct
     1981 gcttgccgac gaagccgccg cgctggaact ctccgcccga ttattgcgct tcaccttccg
     2041 ccgccacgac aacggttacc gccgccggcg catagacgaa gccgccgaca tcctgcaaca
     2101 cgaattcgcc cgtccgctga ccatcgccga aatcgcccgc cgcgtcggac tgaacgaatg
     2161 ctacctgaaa cgctatttca aagcgcaaac cggtgaaacc gtcgccggat gcctgcgccg
     2221 cctgcggctg gaacacgcgc tcgccctcat cgaatccggc agcaccgtcc aagccgccat
     2281 gcacttttgc ggctaccgcc acgcgggacg gttcaacgaa gcgttcagac ggcattacgg
     2341 atttttgcct tcggatgtga agaagtgctg atggggccgg cggcatagat attttgattt
     2401 gaaacaacca ttaaaaaagg tcgtctgaaa aagcagtttc agacgacctt ttttcattcg
     2461 cggcggcagt gccgctcccc gcccttagcc caaaaactct ttcaaaaaca gcaacacgca
     2521 ggcgagttcg tcggcggctt cgcgctgcgt gccgttgccg gtatggccgc cgccgtcagg
     2581 cgagtagagc caagattgcg gcgaggtttc gcgcagtttg gcgtagaact tgagcgcgtg
     2641 ggcgggatgg acgcgatcgt cggacaggct ggtggtaatg agcgcgggcg gataatcgat
     2701 gccgtctgaa agattgtgat acggcgacaa ttcgcccaac cggcgtttgc agacttcgta
     2761 tttttgcgga ttgccgtatt cgtctgtcca gcttgaaccg gcggagagca gcggataacg
     2821 gaacatgtcg gtcagcggca cttcgcacac cagcgcgcct atgctttgcg gttcgcgcac
     2881 gaaggcggcg gcagtaatca gtccgccgtt gctgccgccc tgcaagccga tgtgttcggg
     2941 cgaactgata ccgcgttcgg acaaatcgcg cacgactgcc aataaatcat caacgctttt
     3001 atgtttgctg attccctgcg ccgcctgatg ccagcgcggg ccgaactcgc cgccgccgcg
     3061 gatgttcgcc aatacaaagg cattgccctc ttccagccaa tatttgccaa tgctgcccag
     3121 ataatgcggc aattcgggaa tgccgaaacc gccgtaggca tagaccagcg tcggcgtatc
     3181 gggcgcggcg tttttgccga cgtggaaata agggatgcgt tcgccgtcgg acgacatcgc
     3241 ccaaaactgc cgcacttcga tgccgtctga aacaaactgc tgcggctggc ggcgcatgac
     3301 ggtcagttcc atcacgttca aatccaatgc aaacagcgtc agcggcgtgg tgaaatcgct
     3361 ggcggcaagg taaactacgt cgccccccca cggttggtcg gtcatttcca acgcgcccga
     3421 aggcaggcgc ggcaactcgg tttcctgcca tttgccatca gtaaaacgcc acgctttcag
     3481 acgaccctgt acgttttcca gcaggctcgc cacgacaaaa cgcttggtcg tttccacgct
     3541 ttccaatgcc tgcgtttcat caggcgcaaa caaaagctgc gccgcgccca attcgcctcg
     3601 gtttaatttt actgccacca atgcgccgct cggatagctt tggtttgcgc ggtgccagtc
     3661 tttacgcaat gtcagcaaaa gatgccccgc cagatagccg accacgtcgc aatcggcagg
     3721 caggttcaac ggtttcgcct cgccttcggc tgacacctgc aaataggttt tggtgtaaaa
     3781 accgtccgac gcttcaatca aatcaatcgg cgaaccctgc ggatcgaggt aacgccacgc
     3841 gttcaccatc atcccgtctt cgccgatttg atacaccggc aggctttcct cgaaactttt
     3901 gccgcgttcc accagccaca cctcgcgcgg atagcccgat tcggtcaact gccgctcgtc
     3961 ccaggccgga cacacccaca cgctgttttc atcgcgccac gacacatagt ttttgcctgc
     4021 cggaaagtga aaaccgcctt cgaccaattc ccctgcttcc aaatccactt ccagcgtgta
     4081 cgccgtatcg ccgcctgatt tgctcaaagt caacagcgcg cggttgggct gttccaccaa
     4141 gtgcgacacg ccgcccaaat acacatcgtc gccgagcagc tcgtcgaaat ccgccactga
     4201 aaacaggatt ttccactcgg gatagccgga acgatacgtc gccgccgtgc acacgcggta
     4261 cacgcccttc ggatattccg cgtcctgatg gaaatggtac atccgcgcac ggtgttcctg
     4321 acaaaacgga atctgccgcg tgtcctgcaa ctgcgccaaa ataccgtcag acaatgcgcg
     4381 cgccttgtcg ttgtttaaaa aacgcgcgcg cgtttcggca ttcgcttcag cagcgaagtt
     4441 ttgcgtttcg gcggaatcga ggtcttcaaa atggcggtag gggtcggggt aggatttcat
     4501 cggggtcctt gagggttggg caggtcttta taatcgggga aatgccgtct gaaacggggt
     4561 tcagacggca tttctgcggc ggctttccgt tgcggtcagc ggtgcaggcg acgtaccaga
     4621 atatgcgagt ttcgtccgtt gctgcggtag tctttgcgcc gcgtttcggg caactcgtct
     4681 tccgatgccg tctgaaagcc gcgttcggca aaccattcgc cggtatttgt ggacagtgcg
     4741 aacagcctgc ttatgcctat gccgcgcgcc ttatcgataa tgtgggcaag caggcgttcg
     4801 ccgtagccgc cgtcctgtgc ctgcggcgag acggcaaggc aggcgatttc gccgtaatcg
     4861 gcttcggcaa aggttttcag ggcggcgcaa ccgtacaggt tgccgtcgtg ttcgaggatg
     4921 gaaaattcgg aaatgtggtt ttcgaggtat tcgcggctgc ggtgcagcag gatgccctgt
     4981 tcttccagcg ggcggatgag ggcggcaatg tgcgggatgt cgccgctgtg cgcctgccgg
     5041 atggagacga aggcttcttt ggcaatggac gtgccgatgc cgttgcgagt gaagagttct
     5101 tgcagcaggc tgccgtcggc ggcaccgttg aggatttgga cgcgatgcac gccgccttcg
     5161 agcgcggcaa cggtggacga aatcagccgt cgcgtttcgc cgccggcgtg ttccgccagc
     5221 gattgcgctt cctgtgccga gagggtttcg gcgagcgtgc cgtcggggcg ggaaatgccg
     5281 tctgaaaggg tcaggtaaac gagtttttcg gcctgaagcg agacggcgac ggaggcggcg
     5341 gtttgaagca tatcgagata gaaggtcttg ccgccgtagg aatgtccgag cggcggcagc
     5401 cagacgatat tgcccgcgtc gagttggaaa cggagggcgg cggtgtcggt tttgcggata
     5461 acgcccgcgt attccatatc ggttccgtca atcacaccta tcggacgggc ggtcaggaag
     5521 ttgcccgata cgagcgggac ggaaggcgcg cgcgcgaacc cggaaacgct gccgcacaat
     5581 gcggcttcaa aacggctgcg gacggtgccg gcaaactgct gcgcctgttc gagcgaggtt
     5641 tcgtcggtaa cgcgcaagcc ccggcaataa tgcggcgtgc ggccttgagc ggcggcgtgg
     5701 cggtcgagga agtggcgcgc gccgtggatg aggacgagcc tgatgcccag ttgcgacaaa
     5761 agcccgatgt cggcggcgag cttgtttaag gtatcacctt cgagcaggcg gtcgtctatg
     5821 ccggcgacca gcgttttgcc gcgcatttgg cggatgtagg ggacggcttc gcggaagtgg
     5881 gcgacaaaga ggtcgggcgc gctcatagga tgacgaggta ggaaagctgc ataatgagga
     5941 cgataagggc aaacagggtt gcaatcgtcc agttgaaccc atcctgacgg gcgggcgcgg
     6001 cgggcggtac ggtaacctgc ggtgcggcag caggggtggc ggcggttgcg ggcggaatat
     6061 cgggctgggt tgtaccgccg ttgaggatgc cggcgatttc gtcacgggaa atctgtttct
     6121 tgccgatggc gcgcgtgccg atacggtgaa cgagtttgac atccgaaaca gcctcgggca
     6181 aatcgttgaa tatgggttct ttcgtgcttg ccagatggtc tttggcttta aacagtcctt
     6241 cgcatttttg gcagacgacg aagccttggg cgacattgag ttgggtttct ttgacccaga
     6301 gacgggtttt gcagtggggg cagaaacagg cgggcatggt atttcctcgt gtgtgtcagg
     6361 tttgatgccg tctgaggcgt aaggcggttc agacggcata tgctttgttt ttattctacg
     6421 ccgtactgcc gacggtaggc tcggacgggt tcgaggaact gtccgaattc ggggttgttt
     6481 tgcaacagaa taaacaaatc gttcaggctg gcgatggggg cgacgggcag gccgtattgt
     6541 ttttccactt cctgaaccgc gctcaattca cccgtgcctt tttccatgcg gtcgagcgcg
     6601 atggcgacac cggcgggggt tgcaccctcc gcttcaatca gtttgatcga ttcgcgtacg
     6661 gatgtgccgg cggaaatcac gtcgtcgata atcagcacgc ggcctttaag cggcgcaccg
     6721 accaacacgc cgccttcgcc gtggtctttg gcttctttgc ggttgtaggc aaacgggacg
     6781 ttcacgcctt tttccgccag catcatcgcg gttgccgccg ccaaaataat gcctttgtag
     6841 gcgggaccga acagcatatc gaatcggatg ccgctttcaa tgatggattg tgcgtaaaat
     6901 tttgccagtt gcagcgtgga caagccgtcg ttaaagaggc cggcattgaa gaaatagggc
     6961 gaccgccgtc ctgccttggt ggtaaattcg ccgaatttca aaacattttg ggcgagggag
     7021 aatttgagga aatcttggcg gaaatcggtc attttgtgct ttctgtcaga tattgggacg
     7081 cagttgcgat tctaccgccc cgtgcggcgc gcttcaactg ccgccgcctt gccgcaaggc
     7141 gcgtttcaac tgtttcaaca cctgccaggc gcgggcttta agttcgggtt ggcgcaacag
     7201 ccgggcggga tggtcgatga tgaagaaggg acggctgccg cacaaagttt caatcatcgt
     7261 ttgccgttca ggctggacaa aagcctgccc gaggaaaagg acggcggggg cacggcagcc
     7321 gtcgagttcc ctggcgattt gacccagcgc attcgcgacg gcctgttcag acggcatcgg
     7381 gttgccgacg gcggcggttt tcacccagct ggtcttgtgt acataagcgg catccagtcc
     7441 tacggctttg agtatgttgt caagcaggac acccgctttg ccgtggaaca gctgcccgta
     7501 aaccgcatcc tcgatcggtg ggcaaaggct gacgacggca agtttggtta tgcccgaagc
     7561 ggcgggaaca ggggcgatgc cgtctgaaag gccgggcaga ggcgtttcgg tttcaggcgc
     7621 gggtttgcgc gtaggtacgg cggcggtttc caacgctttc atcgtttcga gccgcgcctg
     7681 accgttatgg ggttgggaag ggcggatcgg ggcggcgcgg acggtttgag gacgtgcctg
     7741 tgccggggtt gcgggtgtgt ttttgggcgg caggacggca gcggcctgtt tcagccacat
     7801 cggacccaaa cccaaagctt cgtgcaggtg gaggtagcgc gcgcttaaca tattttctcc
     7861 atcaagacgg catcttcggt tttaccgtcg gctgtacggt aatagttttt ccgcctgccc
     7921 gcaatgctga agccgtgcgc cgcgtacagt gcctgcgcgg cggtgttgcc tgcgcggact
     7981 tcgagcagca ggcgttgcgt gccttcgggc aggtgtgcgt accaatattc gagcagggcg
     8041 gacgcaattc cttggcggcg gcattcgggc gcggtggcaa tcaggtgcag ttcggattcg
     8101 tcgggcaggt tctgccaaac gataaaggcg gcaatcccgc cgtctttttc cgcaaggaaa
     8161 acctcttcgg acggcgaaac caatgcggac tcaaattggc gttgcgtcca cgcggacggg
     8221 ttgcagacgg catcgagcac ggcgagtgcg gcgcagtcgg acggtgaggc gcggcggatg
     8281 ttcatgttcg ggctttccgt tccgcctgtt ctttggcggt caaggcgatt ttgtcgcgga
     8341 cgtagagcag ctcggcgtgt gccgcagcga cggcgggaaa accgccttca gccgccagat
     8401 tgaggaagtc ggcggcggtc ggcatatcgg gtttgcctga aaacggcggc gtttcttcca
     8461 acgcgaacgc gctgcctatg ccgtctgaaa aggcacatcc ctccggcagc cggatgtctg
     8521 ccgcccgccc gacctgataa tcgctcaaac ggcggcggtt cagcgtgtcg aaccacgcat
     8581 aaaacacttc gcccatacgc gcgtccgcag cggcgagtat gcagctttgc ggcggcggca
     8641 gcgaggcggc ggcatcgagc gtggggatgc cgattaaagg cgtatcaaac ggcgttgcca
     8701 aaccttgcgc cacgccgatg ccgatacgca gtcccgtaaa cgcgccgggg cctttcgcat
     8761 aaacaatcgc ccccaaatcg gcggcggtaa tgcccgcatt tcggaatagg gtgcggattt
     8821 ccggcagaat cagttcggat tggcggctgc cggcctcttg atggaacagg tgggtttcgc
     8881 cgtcggcgcg cagcgcgagc gacaaatagg aagtaccggt atcgatggcc aggacgggac
     8941 ggttaaaatc agcttgcatg gtatggttct cgttggttca gacggcatta tatagtgaaa
     9001 tcagcttgcc tgccgtgccg tcgtgtccta gggcggtatg gcgcaaaaat gccgtccgaa
     9061 cggtaaatta ttgtgttcgg acggcatttt cacaatggtt actgcttggc ggcaagaccg
     9121 atatggtgta tgccgtttgc ggtttccact tccgcgctgc cggcaacttc ctgggcttgc
     9181 ccgccaaaga taccgaggga gtaactgcct ttctcgtctt ggttgtaaag gacggaaccg
     9241 ctgataacgg catggcgttt ttcatccggc ttgatatagg cggtggcaag ctcgacattg
     9301 agttcgggcg atttcaaatg ttcgattttg ccgtgtccct gcttggcggc gaaatctata
     9361 gtataggtca gttttccgcc agcatcgtct gaaccgaacg ccgtcccgcg atatgtcgca
     9421 ctgccgcctt tgggaagctt gtcaaaagat gtatgttcgc ccgctatgtc gccgattctg
     9481 aactggcgtt tcgcaaccat cttcccggaa tcctccgagt cttgtacttg ctcggtctga
     9541 agggcggtta aggcggaatg gctttgtttg tacacttgga actctccgct ctccaaggta
     9601 atgagcttcc cgtccacttc gatttgacgg ataaagtcga agcggctgac cttgtcgttc
     9661 ttcaatttgc ccgtattaag gctgtcgccg tttccataag ttttttccgc accttgtgcc
     9721 gccagcttca gtttctcgtt tttcctgacg gactgatcca gcgttaaaga ctgcaaacct
     9781 ttgtctttat ggtcgagcgg tgcggttagt gcatcggcaa gccccgcacc gatgtcggcg
     9841 gcgacaccgc cccctccgct gctgcaggcg gtcagaatca gggcggcggt cagagaaaag
     9901 cagcagaagg cagttcggtt cacaggttta ctcctagtca tacgcagaat agataatata
     9961 taaacatttt ggttatggta tctttttttg catactgcat caatgaggca ggtcaaagaa
    10021 gcaaaaatca aatgccgtcc gaacaacggt tcagacggca ttttgtttac aagcaacctg
    10081 ttatttgacg atttggttca attcgccctt ggcataacgg tttgccattt tttccaagga
    10141 aaccggtttg attttgcctg cctgaccttc gcaaccgaac gcgaggtagc ggtcgaggca
    10201 gatttgcttc atcgcttcaa tggttttgct caaatatttg cgcggatcga agtcggacgg
    10261 gttttcggca aggtagcggc gtaccgcgcc ggtggaagca aggcgcaagt cggtatcgat
    10321 gttgactttg cgcacgccgt gtttgatgcc ttcgacgatt tcttcaaccg gcacgccgta
    10381 ggtttcaccg atattgccgc cgtattcgtt gatgactttc agccattctt gcggaacgga
    10441 gctggaaccg tgcatcacga tgtgtgtatt gggcagggct tggtggattt ctttgatgcg
    10501 gtcgatacgc aatacgtcgc ctgtgggcgg acgggtgaat ttgtatgcgc cgtggctggt
    10561 gccgacggca atcgccaatg cgtcaacacc ggtatcttta acgaaacgca cggcatcttc
    10621 aacgctggtc agcatttggt cgtgcgaaag tttacccact gcgcctacac cgtcttcttc
    10681 gcctgcttcg ccggtttcga ggttgcccaa tacgccgatt tcgccttcga cggatacgcc
    10741 gcaagcgtgg gagaaattaa ccacggtacg ggtggcgttg acgttgtatt cataagaaga
    10801 aggggttttg ccgtcttcca tcagcgaacc gtccatcatc acggaggaaa agcccagttg
    10861 gatggagcgt tggcacacgt cgggcgatgc gccgtggtct tggtgcatca cgacggggat
    10921 gtgcggaaat tcttcgactg ccgccagaat caggtggcgt aaaaacggcg cacccgcata
    10981 tttgcgcgca cctgcactcg cctgtacgat gacgggcgcg ttgacttggt cggcggcttc
    11041 cataatggcg cgcatttgtt cgaggttgtt gacgttgaac gcgggcaggc cgtagctgtt
    11101 ttcggcagca tgatcaagca gttggcgcat ggatacgagt gccatttgtg tctccttggg
    11161 caataggtaa ataaggcgga ttataatgtt ttttgcggca aaaaaccata aacggctcat
    11221 tgattttatg ttaacgataa cggcgggcgg acgggtcgga ttttgggcgg ttcggggttt
    11281 tggtgtgtcg tttatgataa tgttttcact gtttttttga tatttgtgtg gggcggttat
    11341 ggttttggac gggtttgcgg cgcattttga cgcttatttg gaaaacatcg tgcgcgaggg
    11401 caagtcggag cacacggtgg cggcataccg gcgcgatttg gaagaactgt ttgcgctgtt
    11461 ggcacaaatg ccgtctgaag ctgaaggcgg cgtgccgcag ggcttgtcgc aacgcgattt
    11521 tacggcggcg ttgcggcggc tgtcgcagcg cggtttgaat gcgcggacgc tggcgcgcaa
    11581 gctgtcgtct tggcggcagt attgcgcctg gctggtcaaa cgcgggctga tgcgcgccga
    11641 cccgaccgcc gacatcaaac cgccgaagca gcccgagcgc gtacccaaag cactgccgca
    11701 ggaatggctg aaccggatgc tggatttgcc cgtggacggc ggcgacccgc tggcggtgcg
    11761 cgatcacgcg ctgttcgaac tgatgtacgg cagcggtttg cgcgtgagcg agatacacgg
    11821 cttgaatgca gatgatgtct atttggacga ggcgtgggta cacgttaccg gcaaagggcg
    11881 caagcagcgg caggtgccgc tgaccggcaa aagcgtggaa gccctgaaaa actatctgcc
    11941 gctgcgtcag acggcatcgg acggcaaagc cctgtttacc ggcaggaacg gcacgcgcct
    12001 gagccaacgc caaatccaaa aacgtttggc gcaatgggcg gcgcaaaacg gcgacggcag
    12061 gcacgtttcg ccgcatatga tgcgccacag ctatgccgga cacctgttgc aggcttcgcg
    12121 cgacatccgg gcggtgcagg agctgctcgg gcacagcagc ctttcgacca cgcagattta
    12181 caccaaactc gatttcgacc acatcgcccg cctgtatgac gaagcccacc cgcgcgccaa
    12241 gcggcaggac gaatgacata cggcaaaatc agccgtcagc cgcacgctct tgatatataa
    12301 ttggtcgttg cacctggacg acacataaaa aagacacacc atgaacccaa gccccctact
    12361 cgacctgatt gacagcccgc aagatttgcg ccgtctggac aaaaaacagc tgccgcgcct
    12421 tgccggcgag ttgcgcacct ttctgctgga atctgtcggg cagaccggcg ggcatttcgc
    12481 cagcaatttg ggcgcggtcg aactgaccat cgccctgcac tatgtgtacg acacgcccga
    12541 agacaagctg gtgtgggatg tcggacacca aagctatccg cacaaaatcc tgacaggcag
    12601 gaaaaaccag atgcacacca tgcgccaata tggcggtttg gcgggttttc cgaaacgttg
    12661 cgagtccgag tacgacgcgt tcggcgtggg gcattcctcc acctccatcg gcgcggcgtt
    12721 gggcatggcg gcggcagaca aactcttggg cagcgaccgc cgcagcgtcg ccatcatcgg
    12781 cgacggcgcg atgacggcgg ggcaggcgtt tgaagccttg aattgcgcag gcgatatgga
    12841 tgtggatttg ctggtcgtcc tcaacgacaa cgaaatgtcg atttccccca acgtcggcgc
    12901 gctgccgaaa taccttgcca gcaacgtcgt gcgcgatatg cacggcctgt tgagtaccgt
    12961 caaagcgcaa acgggcaagg tattagacaa aatacccggc gcgatggagt ttgcccaaaa
    13021 agtcgaacac aaaatcaaaa cccttgccga agaagccgaa cacgccaaac agtcgctgtc
    13081 tttgtttgaa aacttcggct tccgctacac cggccccgtg gacggacaca acgtcgaaaa
    13141 tctggtggac gtattgaaag acttgcgcag ccgcaaaggc cctcagcttc tgcacgtcat
    13201 caccaaaaaa ggcaacggct acaaactcgc cgaaaacgac cccgtcaaat accacgccgt
    13261 cgccaagctg cctaaagaag gcgcggcgca aatgccgtct gaaaaagaac ccaagcccgt
    13321 cgccaaaccg acctataccc aagtgttcgg caaatggctg tgcgaccggg cggcggcaga
    13381 cgagcgtttg atcgccatca cgcccgcgat gcgcgagggc agcggactgg tggagtttga
    13441 acaacgattc cccgaccgtt atttcgatgt cggcattgcc gagcagcacg ccgttacctt
    13501 tgccggcggt ttggcgtgcg aaggcatgaa gcccgtcgtg gcgatttatt ccaccttttt
    13561 acaacgcgcc tacgaccaac tggtgcacga catcgccctg caaaacctgc ccgttttgtt
    13621 tgccgtcgac cgcgcgggca tcgtcggcgc ggacggcccg acccatgccg gtctgtacga
    13681 tttgagcttt ttgcgctgca ttccgaatat gattgtcgcc gcgccgagcg atgaaaacga
    13741 atgccgcctg ttgctttcga cctgctatca ggcagacgcg cccgccgccg tccgctatcc
    13801 gcgcggcacg ggtacgggcg cgccggtttc agacggcatg gaaaccgtgg aaatcggcaa
    13861 gggcattatc cgccgcgaag gtgagaaaac cgcattcatt gccttcggca gtatggtcgc
    13921 ccccgcattg gcggttgccg aaaaactgaa cgccaccgtc gccgatatgc gcttcgtcaa
    13981 accgatagac gaagagttga ttgtccgcct tgcccgaagc cacgaccgca tcgttaccct
    14041 tgaagaaaac gccgaacagg gcggcgcagg cggcgcggtg ctggaagtat tggcgaaaca
    14101 cggcatctgc aaacccgttt tgcttttggg cgttgccgat accgtaaccg gacacggcga
    14161 tccgaaaaaa cttttgaacg atttgggctt gagtgccgaa gcggtggaac ggcgtgtgcg
    14221 cgcgtggctg tcggatcggg atgcggcaaa ttaagccgcc cggccgcgcc gtcgtctgcc
    14281 gatacaaggc aaaatgccgt ctgaaacgct tcagacggca ttttttcggc gtgagggttt
    14341 aggcttcgac aactttgccg cgcagggaga aggtgtaggc ctcggtgatt tccaaatcga
    14401 tcatttggtt aatcatgtcg ggcgtgccgg tgaagttgac gacgcggttg ttggcggtgc
    14461 gggcttgcag ttggtcgggg tcttttttgg agataccttc gaccaggcag cgttgaaccg
    14521 tgccgaccat ggtttggttg atgcgcgcgg tttcggcttc gatgacttcg ttcaaagctt
    14581 cgaggcggcg cactttttct tcgtgcggcg tgtcgtccgg caggttggcg gcaggcgtgc
    14641 cggggcgcgg actgtaaata aagacaaagc tcaagtcgaa ggcaatgtct ttcaccagtt
    14701 tcaaggtttg ctcgaactcg cgctcggtct cgcccgggaa gccgacgata aagtcggagc
    14761 tgaggcacaa atcaggacgg atggcgcgca gtttgcggat gatggatttg tattccaaag
    14821 cggtgtagcc gcgtttcatc gcgctcaata cgcggtcgga accgctttga atcggcaggt
    14881 gcaggtggga aaccagcttc ggcaggtcgc ggtagcactc gataatcgag tcggtaaact
    14941 cgcgtgggtg gctggtggtg aagcgcatac gttcgatgcc ggggatttcg tggacgatac
    15001 gcagcagggt ggcgaagtcg cagatttcgc cgtcgtccat ttcgccgcga taggcattga
    15061 cgttttggcc taagaggttg atttctttca cgccttgctg ggcaaggttg gcgatttctg
    15121 tcaatacgtc gttgagcggg cgggagaatt cttcgccgcg cgtgtagggg acgacgcaga
    15181 aggagcagta tttggaacag ccttccataa tcgacacgaa tgccgcgccg ccttcgacgc
    15241 gggcgggcgg aaggtggtcg aatttttcga tttcggggaa ggaaatatcg acttgcgaca
    15301 gcccgctggt ttctttgtcc acaatcattt tgggcaggcg gtgcagtgtt tgcgggccga
    15361 aaaccacgtc cacataaggc gcacgtttga tgatgttttc gccttcttgc gaggcgacgc
    15421 agccggcaac gccgatgatg aggccggggt ttttttcttt gagcggacgc acgcgcccca
    15481 aatcggagaa gactttttcc tgtgcttttt cgcgcacgga gcaggtgttg aacaagatga
    15541 tgtcggcttc gtcggcttgg gtaacctgtt cgatgccgcc gtgttcttcg gcgaggacgg
    15601 cgagcatttt gtcgctgtcg tattcgttca tctggcagcc gaaggtgcgg ataaatactt
    15661 ttttcatggt ttgtgtcttt ctcaggcagc cgtaatcgcg gggctgattg ttgttggaat
    15721 gaaaaaattt cagacggcat gacgatgccg tctgaaaatc ggtgcggatt atagcacgat
    15781 gtgggttttg taaacagaat attgttttaa aatatgaatt taatcggtcg ggacggctgt
    15841 ataatgtttg gctttaatgg gaggtgtgta tgaaaccggc tgtgtgggcg gcattgctgc
    15901 tgtgtgcgtg taccagcaat ttcggcgaca gggaacatca gttcctgcgt gatcagacgg
    15961 aagaaaaaat cgtgcagttt gcggttaggg gcaagacgac ccgggcggaa gtggaagcgc
    16021 gtttcgggcg gcaaaatccg ttcggctgtt atgcctatca cgaggtcagc ctgcctattt
    16081 ataatttttt gccgaccaat ttcatttata tgaaatcaga acgccggcat tgggagtggt
    16141 gtgtggatta cgacggggag ggagttgtcc gggactaccg ctttacacat aaagtggaaa
    16201 aagacgagcg ttccgtcatc cgggatgcgg ttggcgtcat ccgcaaagaa gcaggcaaat
    16261 ccttgtcgca acctgaaaaa tgataaaatg ggactttctg cttctaagcc cgaaacctgc
    16321 cgttcagacg gcatttgagg ataaatatga accgtaacga aattttattc gaccgcgcca
    16381 aagccatcat ccccggcggc gtgaattccc ccgtccgcgc attcggcagc gtcggcggtg
    16441 tgccgcgctt tatcaaaaaa gccgaaggcg catatgtttg ggacgaaaac ggcacgcgct
    16501 acaccgatta cgtcggctct tggggtcctg cgattgtcgg acacgcacac cccgaagtca
    16561 tcgaagccgt gcgcgaagct gccttgggcg gtttgtcgtt cggcgcgccc accgagggcg
    16621 aaatcgccat tgccgaacaa atcgccaaaa tcatgccgtc cgtcgaacgg ctgcgcctcg
    16681 tcagctccgg taccgaagcg acgatgaccg ccatccgttt ggcacgcggt tttaccggtc
    16741 gcgacaaaat catcaagttt gaaggctgct accacgggca ttccgacagc ctgctggtga
    16801 aagccggcag cggcctgctg acgttcggca atccttcttc cgccggtgtg cctgccgact
    16861 ttaccaaaca cactttagtc cttgagtaca acaacatcgc ccaattggaa gaagcctttg
    16921 ctcaaagcgg caatgacatt gcctgtgtga ttttggagcc ttttgtcggc aatatgaacc
    16981 ttgtccgccc gaccgaggcc tttgtcaaag ccttgcgcgg attgaccgaa aaacacggcg
    17041 cggtgttgat ttacgacgaa gtgatgaccg gcttccgcgt cgcgctcggc ggcgcgcaat
    17101 cgctgcacgg catcacgccc gacctgacca cgatgggcaa agtcatcggc ggcggtatgc
    17161 cgcttgccgc gttcggcgga cgcaaagaca tcatggaatg tatttccccg ctgggcggcg
    17221 tgtatcaggc aggcacatta tcaggcaacc cgattgccgt cgccgccggc ttgaaaacgc
    17281 tggaaatcat ccggcgcgaa ggcttctatg aaaacctgac tgccctgacc agccgcctcg
    17341 ccgacggtat tgccgccgcc gccaaagcgc acggtatcga attcaccgcc gacagcgtgg
    17401 gcggtatgtt cggtctgtat tttgccagcc atacgccgcg aaactatgcc gatatggcgc
    17461 gatccaatat cgacgctttc aaacgcttct tccacggcat gctcgaccgc ggcattgcct
    17521 ttggcccgtc cgcttatgaa gcaggtttcg tttccgccgc gcatacgccc gagctgatta
    17581 acgaaacggt tgcggttgcg gttgaagtgt tcaaggcgat ggctgaatga tgttttgacg
    17641 gacagagttt ctctgttcga tttgtttggc agattgaagt aagaatgcac accgccgtca
    17701 ttcccgcgca ggcgggaatc cagacctttc agtttctgta atgattgaaa ataacgtgcc
    17761 gaaatcagcc gcacccggca ggaagccgcc gccacctgaa acaatgccgt ctgaagcctt
    17821 cagacggcat tttatcggaa gcgcatcaga aaaaacggat agcgacatat gcggtaaaag
    17881 caagggcaat tacatagata aaccccttta taaattcaaa agtctcatct atatcgctat
    17941 atttttcttt cagttcgtca gattttcgat aacgtttcat tttcaagacc acttcatgac
    18001 caacaagccc gaccttccgg attcccgcct gcgcgggaat gacgggcgtg tatatttttg
    18061 atttcaatct gctgtaaaaa tgccgtctga aatgcaggat ttgcgcttca gacggcattg
    18121 tttgtttctt attgcgcttc ggctctcggg gcggaaatca gaaagtgttc gcggtagtgg
    18181 cgcatttctt cgatgctctc caaaatgtcg tccaatgcct tgtgcgaacc gcgtttgacc
    18241 acgcttttgg caacgggcgg attccagcgt ttggcaagct ctttgagcgt ggaaacgtcg
    18301 aggttgcggt agtggaagta gttttccagt ttcggcatat atttgaccat aaaacgccgg
    18361 tcttggtgga tggagttgcc gcacatcggc gtggcgcgtt cgggtaccca ttccgacata
    18421 aagtccagca gtttctgttc gacttcggct tcggtatgcg acgattcgcg tacgcgctgc
    18481 gtcagccccg tcctaccgtg tgtggcggtg ttccactcgt ccatattgtc gagcagctcg
    18541 tcgctttggt ggatggcgta aacttcggat tgcgccaata cattcaaatc cgagtcggta
    18601 ataatcatcg cgacttcgat aatgcggtcg gtttcgggat taagccccgt catttccata
    18661 tcgagccagc ataggttgtt tttatcctgc atgatgtttc ctttgcttgt cagcgttttg
    18721 tcccgcttaa tcgcgcccat ccttcttcgg tttccgcagg atcgagatcg aaccattggc
    18781 tgtaaatgcc gctgagttct tcgatttgtt cgtccaacaa accggataac acgatgcggc
    18841 cgccttgttt ggtgcgggcg gcaagcattt cgccgagcat acgtaaaggg ttggcgagga
    18901 tgttggcaac aactacgtcg aattgccctt gaggcaggcc gtcgggcagg aagaattgtg
    18961 catcgacgtt gttctgttcg gcgttgtcct tgccggcgcg gacggcttgt tcgtcaatat
    19021 cgacgccgac ggcagaacct gcgccgagtt tgagggcagc gatggtcagg atgcccgaac
    19081 cgcagccgta gtcgaggacg ctttcgccgt ttttgagttg cgtatccagc catttgaggc
    19141 agaggcgcgt ggttgggtgg ctgccagtgc cgaaggcgag gccgggatcg aggcgtaggt
    19201 tgacggcagt gccttcgggg gcttcgtgcc aagagggggt aatccacagg cggtcggaaa
    19261 ttcggatggg gtcgaattgc gattgcgtga gacgcaccca gtcttggtct tcgatggttt
    19321 cgccggtgta tgccaagtct tttaacccgc attcttgtgc ggcgatttgg atgatggcgg
    19381 cggcttcgtc gtgttcgccg aacagggcga tgactttgct ctgctgccag atttgttcgg
    19441 cgggcatacc gggttcgccg aaaatcgcct gttcgttttg cgtgccggcg taggcatctt
    19501 cgatggcggc ggagagtgcg ccgtgttcca tcagcgcgtc ggcgaggcgt tcggcgacgg
    19561 catcgttgac gttgacggtg atttgttggt agggcataac gggctttctt aacagacctt
    19621 cggaataggg tcgtactgta taaggggatg ggtgtgtaac ggaaatgccg tctgaaaacg
    19681 gacaaactgt ttcagacggc atatcggcgg cggtttactt gtcctgtttg gctttgcgat
    19741 cttccagcca gtgttccaaa tagtggatgc tgacgccgcc tttttggaaa cccgcatcgg
    19801 cgaacaggtc gcggtgaagc ggcgtattgg ttttgatgcc ggttaccgcc agctcggcga
    19861 gtgcgacgcg cattttcgcc attgcctgtt cacgcgtttt gccgtgtacg cagattttgc
    19921 cgatcaggct gtcgtagtac ggtgggatgc ggtagccttg gtaaatgtgg ctgtccacgc
    19981 ggataccgaa gccaccgggc aggtggcagc tttcaatcgg gcctgggctg gggatgaagt
    20041 tgtacgggtc ttcggcgttg atacggcact caaacgcgtg gccttcgact tgaatatcct
    20101 tttgtttgta ttgcaacggc aggccggcgg cgatgcgcaa ttgctcttgt acgatgtcca
    20161 cgccggtgat gagttcggta accggatgct cgacctgaac gcgcgtgttc atctcgataa
    20221 agaaaaattc gccgtcttcg tacaggaatt caaacgtacc tgcgccacga tagccgatgc
    20281 gtttgcaggc gtcggcacag gcgttgccga ttttggcgcg ttcttcttca gtgatgaacg
    20341 gagccggtgc ttcctcgatg actttttggt ggcggcgttg cagcgaacag tcgcgctcgg
    20401 caagatatac ggcgttgccg tgttcgtcgg caatcacttg gatttcgacg tggcgcggac
    20461 gttgcagata gcgttccatg taaaccatcg ggttgccgaa tgccgcgcct gcttctgcct
    20521 tggtcatttc gacagattgg aggaggtctt cttttttctc gaccacgcgc ataccgcgac
    20581 cgccgccgcc gccagaggct ttgataatga cgggataacc gactttatcg gcgattttga
    20641 ggatttcttc gccgtcgtcg ggcaatgcgc cgtcagaacc ggggacgcag ggtacgcctg
    20701 ccgctatcat cgcgtgtttg gcggagactt tgtcgcccat caggcggatg gtgtcgggtt
    20761 tcgggccgat aaaggtaaag ccggactgct cgacctgttc ggcgaaatcg gcgttttcgg
    20821 caaggaaacc gtagcccgga tggacagcgt ccgcgcagct tacttcggcg gcggcgataa
    20881 tggcggggac gttaaggtag ctttgcgcgg aagcggcagg gccgatgcac acggattcgt
    20941 cggcgagttt gacgtgcagg ctgtctttgt cggcctcgga atgcacggcg acggtggcaa
    21001 tgcccatttc gcggcaggca cggagtacgc gtaatgcgat ttcgcctcgg ttggcgatta
    21061 aaactttttt cagcatgatg actgttcctg tggttccggc aacccgtccg taaaaaaaag
    21121 gtgcgggaac gctgaagggg gaaagcattt cagacggcat cggaagttta tgccgtctga
    21181 aaacaggatt atccgatgat gaagagcggt tcgccgaatt cgacgggcgt accgttttcg
    21241 accaaaattt ctttgaccgt gccggatttt tcggcttcga tttcgttcat cagcttcatc
    21301 gcttcgatga tgcacagcgt gtcgccggct ttaacctgtt ggccgacttc gacaaaaggc
    21361 gcggcattcg ggccgggtgc gcggtagaac gtgccgacca taggtgattt ttgcgcgtcg
    21421 gacaaatcgc gggcggcagg tgcggcggca ggcgcggatg ccgcaacggg tgcggcggca
    21481 ggcgttacgg ccggcgcggc ggcaggtacg ggcgcggcgt aaacgggtgc ggcggcgatg
    21541 gttcgggtga tgcggacttt ttcctcgcct tcggttactt cgatttcggc gatacccgat
    21601 tcttcaacca aatcaatcag tttttttaat ttgcgcaaat ccatttctgt tcctttaaag
    21661 gctgccggtg ttccggcggg ttgttgcgtt ggtttttcag aaaacagccg gcttgcggca
    21721 acggcgcaaa ccgggcggaa tggtgttcgg atacggtttt cctgtggtgg aaaacaaccg
    21781 tgagaatgtg tttattttcc cgaagttgtc ggtaaatgtc cagtaaaata tcaaaaaacg
    21841 gcggtttttg ggggaaatgt gcaggaagtt tgattttgcg cacaagatgt tccaggttaa
    21901 gagtgtttat tctaatctgt tggtttttcg gacaaagatg ccgtctgaaa aagggctaaa
    21961 gtgcgtataa tggcggcttg cccgaacgag agtgtaaaaa tggatatttc agattttgat
    22021 tttaccctgc ccgaaaagct gattgcccag catccgcccg aagtgcgcgg cagcagccgc
    22081 cttttggttg cgttgcccga tatgccgctg caagaccggg tgttcggcga tttgccggat
    22141 tatgtcgagg cgggcgacgt tttggtgttc aacaacacca aagtcatgaa ggcgcggctg
    22201 tttgggcaga aagacagcgg cggcaggatc gaagccctga ttgagcgcgt gttggacaac
    22261 cataccgcac tggcgcacat ccgttcgtcc aagtccccca agcccggtat ggggctggtg
    22321 tttgaaggcg gcatccgtgc cgtgatggtc gggcgtgagg gcgaactgtt ctgcctgcgt
    22381 tttgaaggcg gtcaaaccgt ttacgaactt ttggaacaga acggacacct gcccctgccg
    22441 ccttatatcg aacgcgccgc cgacgcggac gacgacagcc gttatcaaac cgtttatgcc
    22501 aaatatcagg gtgcggtcgc cgcgccgacg gcgggtctgc attttacgga agaacttttg
    22561 cgccgcctga aagacaaagg cgcagtaacc gcagaagtaa ccctgcacgt cggtgcggga
    22621 acgttccagc ccgtgcgtgt ggaaaaaatc gaagaacaca aaatgcacag cgaatggttt
    22681 gaagtgccgt ctgaaaccgc cgccgccgtt gaggcggcaa aagcacgggg gaacaaagtc
    22741 tgggcggtcg gcacgacttc catgcgcgcc ctcgaatctg ccgcgcgtgc gacgggtcgt
    22801 ctgaaagcgg ggcagggcga caccgatatt ttcatcacgc cgggctaccg ttttaatgtt
    22861 gtcgacaggc tggttaccaa ttttcatttg ccgaaatcga cgctgctgat gcttgtcagc
    22921 gcgttttcgg gtatgggtca tatccgcgcc gcgtatcgtt atgcggtcga acgcgaatac
    22981 cgtttcttca gctacggcga tgcgatggtt ttggggcgga acgaaggggt ggtacgttag
    23041 aacagcccca agtctattgg gggaagacaa gtgccgtctg aacggacgtt cggacggcat
    23101 tcttttgttg atttgaatga aaggaactgt atgacataga ttcaggcgcg agaggcgaga
    23161 aactcagtca ttgttgatga cgcgcaatac gttaaaattc attccgtttt ggtcgtataa
    23221 acatattaca tacgccattt ttttgtattc aaggctgcct gaaaacagtg ggcaagaata
    23281 tctaacccaa cgaaaagctg aaaaagcaac aaaaaataat ctgcacctta gtcggttggt
    23341 tagtccaacc ttaggggtgc agaccattca atctttcagg ctgcctttgt ttgtttataa
    23401 gataaatttt tagccgaaca ctttttccaa gtgtgcctga taatctgcca agtatttttc
    23461 cacttg
//
LOCUS       DAXHSF010000014        23300 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.014, whole genome shotgun sequence.
ACCESSION   DAXHSF010000014 DAXHSF010000000
VERSION     DAXHSF010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23300
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.014"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            44..1720
                     /gene="pilB"
                     /locus_tag="ACFM8S_000440"
     CDS             44..1720
                     /gene="pilB"
                     /locus_tag="ACFM8S_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HFC6313564.1"
                     /translation="MSVGLLRILVQNQVVTVEQAEHYYKESQAGKEVLPMLFSDGVIS
                     PKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQMVEFHCVPVFRRGDKVFFAVSD
                     PTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
                     YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPI
                     AVRGQLASRIKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMR
                     ILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNT
                     ESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETA
                     DIAIKAAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSS
                     CKQEVERPSASALKEVGFTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEE
                     MQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND"
     gene            complement(1805..2014)
                     /locus_tag="ACFM8S_000441"
     CDS             complement(1805..2014)
                     /locus_tag="ACFM8S_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFC6313565.1"
                     /translation="MAESRQTRLQVKCPTCQTAVVWEPENAFRPFCSQRCKLIDLGGW
                     ADGKYTVSGQTESLPEISEPDMAYR"
     gene            complement(2007..2639)
                     /gene="coaE"
                     /locus_tag="ACFM8S_000442"
     CDS             complement(2007..2639)
                     /gene="coaE"
                     /locus_tag="ACFM8S_000442"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261706.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFC6313566.1"
                     /translation="MTVWVGLTGGIGSGKSAAAQCFADLGVPRIDADATAHSLTASDG
                     IALPEIRRLFGDTVFDTQGLLRRDILRKEIFASPSRKALLESVMLPLIFSEIKKQQET
                     FADAAYGIVEIPLLTEKRQFISLIRRVLTISAPLEKRIGRVMARSGLTRGEVAAVISH
                     QASESERLLLADDVLLNDGSLKSLREKTMRLHAFYSGIFASKPTQGKHNG"
     gene            complement(2641..3501)
                     /locus_tag="ACFM8S_000443"
     CDS             complement(2641..3501)
                     /locus_tag="ACFM8S_000443"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFC6313567.1"
                     /translation="MSDLSVLSPFAVPLAAVFGLLVGSFLNVVIYRVPVMMERGWTVF
                     AKEYLNLPLTEEESRTFNLMKPDSCCPKCRVPIRAWQNIPIVSYLLLRGKCASCQTKI
                     SIRYPLIELLTGVLFGLVAWQYGWSWITLGGLVLTAFLISLTFIDADTQYLPDSMTLP
                     LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSLWLLCAVYKLLTGKTGMGNGDFKLIAAL
                     GAWLGISALPVLIFVSSLIGLVAAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRA
                     VNWWLTHPVL"
     gene            complement(3575..4807)
                     /gene="pilG"
                     /locus_tag="ACFM8S_000444"
     CDS             complement(3575..4807)
                     /gene="pilG"
                     /locus_tag="ACFM8S_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus assembly protein PilG"
                     /protein_id="HFC6313568.1"
                     /translation="MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARK
                     KLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLSTMIKAGLPLMQAFEIVARGHGN
                     PSMTEMLMEIRGEVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
                     KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPALTQTV
                     MDMSDFFVSYGWMVLIALGFAIYGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTI
                     ARWGRTTATLIAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRVIQGLSMTSGMRATE
                     LFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIG
                     TLLVAMYLPLFNLGNVVA"
     gene            5317..6960
                     /gene="pgi"
                     /locus_tag="ACFM8S_000445"
     CDS             5317..6960
                     /gene="pgi"
                     /locus_tag="ACFM8S_000445"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFC6313569.1"
                     /translation="MNAFTRAWYALERHNQDTRHVLLRDRFACEPDRFERMHERLDGM
                     LFDYSKNRLGEDTLQLLCRLAETADLEGKMRALRTGAKVNGSEGRAALHTALRLPDGA
                     DAVYADGRDVLPEIRRELNRALKFAHSLDDGSYQGTTGKRITDFVHIGIGGSDLGPAM
                     CVQALEPFRRHIAVHFAANADPACLDAVLCRLNPETTVFCVASKSFKTPETLLNAQAV
                     KAWYRGAGFSESETACHFCAVSADTAAAAAFGIAAERVFAMYDWVGGRYSVWSPVGLP
                     VMVAVGGARFRELLAGAHAMDRHFFSTPTRHNIPVLMALIAVWYNNFQQADGQTAVPY
                     SHNLRLLPAWLNQLDMESLGKSRASDGSPAACKTGGIVFGGEGVNCQHAYFQLLHQGT
                     RLIPCDFIVPMTAQGVEDGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERER
                     LAPHKEFPGNRPSNSILLDRLTPYNLGMLMAAYEHKTFVQGVIWDINPFDQWGVEYGK
                     QLAKTIIGELEGGTSVHDASTEGLMAFYRECRLKGGGAA"
     gene            6930..7121
                     /locus_tag="ACFM8S_000446"
     CDS             6930..7121
                     /locus_tag="ACFM8S_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313570.1"
                     /translation="MPSERRRRGIKAIPAACPIPKRQCSATARVLLTNMRLRGTIAHS
                     FQMNILPENTGKRPTKHLT"
     gene            7131..7952
                     /gene="dapD"
                     /locus_tag="ACFM8S_000447"
     CDS             7131..7952
                     /gene="dapD"
                     /locus_tag="ACFM8S_000447"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFC6313571.1"
                     /translation="MSLQNIIETAFENRADINPTTVTPEVKEAVLETIRQLDSGKLRV
                     AERLGVGEWKVNEWAKKAVLLSLRIQDNEILNDGVNKYFDKVPTKFADWSEDEFKNAG
                     FRAVPGAVARRGSFVAKNVVLMPSYVNIGAYVDEGAMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQASPTIIEDNCFIGARSEIVEGVIVEEGSVISMGVFIGQSTKIFDRT
                     TGEIYQGRVPAGSVVVSGSMPSKDGSHSLYCAVIVKRVDAQTRAKTSVNELLRGI"
     gene            8107..8892
                     /gene="fabI"
                     /locus_tag="ACFM8S_000448"
     CDS             8107..8892
                     /gene="fabI"
                     /locus_tag="ACFM8S_000448"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFC6313572.1"
                     /translation="MGFLQGKKILITGMISERSIAYGIAKACREQGAELAFTYVVDKL
                     EERVRKMAAELGSELVFRCDVASDDEINQVFADLGKHWDGLDGLVHSIGFAPKEALSG
                     DFLDSISREAFNTAHEISAYSLPALAKAARPMMRGRNSAIVALSYLGAVRAIPNYNVM
                     GMAKASLEAGIRFTAACLGKEGIRCNGISAGPIKTLAASGIADFGKLLGHVAAHNPLR
                     RNVTIEEVGNTAAFLLSDLSSGITGEITYVDGGYSINALSTEG"
     gene            complement(9077..10075)
                     /gene="ilvE"
                     /locus_tag="ACFM8S_000449"
     CDS             complement(9077..10075)
                     /gene="ilvE"
                     /locus_tag="ACFM8S_000449"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFC6313573.1"
                     /translation="MSRPVPAVFGSVFHSQMPVLAYREGKWQPTEWQSSQDLSLVPGA
                     HALHYGSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETQAYLDALVE
                     LVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPV
                     KILVETEHIRCAPHMGRVKCGGNYASAMHWVLKAKAEYGANQVLFCPNGDVQETGASN
                     FILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTVSERNFTVDELKAAVENGAEA
                     ILTGTAAVISPVTSFVIGGKEIEVKSQERGYAIRKAITDIQYGLAEDKYGWLVEVC"
     gene            10385..10699
                     /locus_tag="ACFM8S_000450"
     CDS             10385..10699
                     /locus_tag="ACFM8S_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly LapA
                     domain-containing protein"
                     /protein_id="HFC6313574.1"
                     /translation="MKLIYTVIKIIILLLFLLLAVINTDAVTFSYLPGQKFDLPLIVV
                     LFGAFVVGIVFGMFALFGRLLSLRGENGRLRAEVKKNARLTGKELTAPPAQNAPESAK
                     QP"
     gene            10710..11879
                     /gene="lapB"
                     /locus_tag="ACFM8S_000451"
     CDS             10710..11879
                     /gene="lapB"
                     /locus_tag="ACFM8S_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFC6313575.1"
                     /translation="MDNELWIILLPIILLPVFFAMGWFAARVDMKTVLKQAKSIPSGF
                     YKSLDALVDRNSGRAARELAEVIDQQPQSYDLNLTLGKLYRQRGENDKAINMHQTLLD
                     SPDTTGAKRARVLFELAQNYQSAGLVDRAEQIFLSLQDGEMAREARQHLLNIYQQDRD
                     WEKAIETAQLLSHDEQTYQFEIAQFYCELAQAALFKSNFDVARFNVGKALEANKKCTR
                     ANMILGDIEYRQGNFPASVEAYAAIEQQNHAYLSMVGEKLYDAYAAQGKPEEGLNRLI
                     GYMQTFPELDLINVVYEKSLLLRGEKEAAQTAIELVRRKPDLNGVYRLLGLRISDMDP
                     TWKADADMMRSVIGRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEV"
     gene            11904..12320
                     /gene="gloA"
                     /locus_tag="ACFM8S_000452"
     CDS             11904..12320
                     /gene="gloA"
                     /locus_tag="ACFM8S_000452"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFC6313576.1"
                     /translation="MRLLHTMLRVGNLEKSLDFYQNVLGMKLLRRKDYPEGRFTLAFV
                     GYGDETDSTVLELTHNWDTERYDLGNAYGHIAVEVDDAYEACERVKRQGGNVVREAGP
                     MKHGTTVIAFVEDPDGYKIEFIQKKSGDDSVAYQTA"
     gene            complement(12493..15006)
                     /gene="tspA"
                     /locus_tag="ACFM8S_000453"
     CDS             complement(12493..15006)
                     /gene="tspA"
                     /locus_tag="ACFM8S_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_164729270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark-like protein
                     TspA"
                     /protein_id="HFC6313577.1"
                     /translation="MKNNRQIKLIAASVAVAASFQAHAGLGGLNIQSNLDEPFSGSIT
                     VTGEEAKALLGGGSVTVSEKGLTAKVHKLGDKAVIAVSSEQAVRDPVLVFRIGAGAQV
                     REYTAILDPVGYSPKTKSALSDGKTHRKTAPTAESQENQNAKALRKTDKKDSANAAVK
                     PAYNGKTHTVRKGETVKQIAAAIRPKHLTLEQVADALLKANPNVSAHGRLRAGSVLHI
                     PNLNRIKAEQPKPQTAKPKAETASMPSEPSKQATVEKPVEKPEAKVAAPEAKAEKPAV
                     RPEPVPAANTAASETAAESAPQEAAASAIDTPTDETGNAVSEPVEQVSAEEETESGLF
                     GGSYTLLLAGGGAALIALLLLLRLAQSKRARRTEESVPEEEPDLDDAADDGIEITFAE
                     VETPATPEPAPKNDVNDTLALDGESEEELSAKQTFDVETDTPSNRIDLDFDSLAAAQN
                     GILSGALTQDEETQKRADADWNAIESTDSVYEPETFNPYNPVEIVIDTPEPESVAQTA
                     ENKPETVDTDFSDNLPSNNHIGTEETASAKPASPSGLAGFLKASSPETILEKTVAEVQ
                     TPEELHDFLKVYETDAVAETAPETPDFNAAADDLSALLQPAEAPSVEENITETVAETP
                     DFNATADDLSALLQPSEVPAVEENAAEIVADDLSALLQPAEAPAVEENVTETVAETPD
                     FNATADDLSALLQPSEVPAVEENAAETVADDLSALLQPAEAPAVEENAAEITLETPDS
                     NTSEADALPDFLKDGEEETVDWSIYLSEENIPNNADTSFPSESVGSDAPSEAKYDLAE
                     MYLEIGDRDAAAETVQKLLEEAEGDVLKRAQALAQELGI"
     gene            15314..15844
                     /locus_tag="ACFM8S_000454"
     CDS             15314..15844
                     /locus_tag="ACFM8S_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFC6313578.1"
                     /translation="MKFVSDLLSVILFFATYTVTKNMIAATAVALVAGVVQAAFLYWK
                     YKKLDTMQWVGLVLIVVFGGATIVLGDSRFIMWKPSVLFWLGALFLWGSHLAGKNGLK
                     ASIGREIQLSDAVWAKLTYMWVGFLIFMGIANWFVFTRFEAQWVNYKMFGSTALMLVF
                     FIIQGIYLSTCLKKED"
     gene            15847..16137
                     /locus_tag="ACFM8S_000455"
     CDS             15847..16137
                     /locus_tag="ACFM8S_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFC6313579.1"
                     /translation="MEYFMLLATDGENVYEARMAARPEHLKRLETLKSEGRLLTAGPN
                     PLPEDSNRVSGSLIVAQFESLDAAEAWAEDDPYVHAGVYSEVLIKPFKAVFK"
     gene            16137..16415
                     /locus_tag="ACFM8S_000456"
     CDS             16137..16415
                     /locus_tag="ACFM8S_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HFC6313580.1"
                     /translation="MPAVDLIRERLQTLDPLVLEIGDESHLHKGHAGNTGGGHYAVLV
                     VSGRFEGVSRLNRQKTVKSLLKDLFSGGMIHALGIRAATPDEYFHTAD"
     gene            16464..17330
                     /locus_tag="ACFM8S_000457"
     CDS             16464..17330
                     /locus_tag="ACFM8S_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC6313581.1"
                     /translation="MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFR
                     AENSRAEDTPQLRQSLLENEVVNTVVAQEVKRLKLDRSAEFKNALAKLRAEAKKSGDD
                     KKPSFKTVWQAVKYGLNGEAYALHIAKTQPVSEQEVKAAYDNISGFYKGTQEVQLGEI
                     LTDKEENAKKAVADLKAKKGFDAVLKQYSLNDRTKQTGAPVGYVPLKDLEQGVPPLYQ
                     AIKDLKKGEFTATPLKNGDFYGVYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRAVG
                     ALLGKANIKPAK"
     gene            17401..18159
                     /locus_tag="ACFM8S_000458"
     CDS             17401..18159
                     /locus_tag="ACFM8S_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC6313582.1"
                     /translation="MKQKKTAATVIAAMLAGFAAAKAPEIDPALVDTLVAQIMQQADR
                     HAEQSQKPDGQAIRNDAVRRLQTLEVLKNRALKEGLDKDKDVQNRFKIAEASFYAEEY
                     VRFLERSETVSESALRQFYERQIRMIKLQQVSFATEEEARQAQQLLLKGLSFEGLMKR
                     YPNDEQAFDGFIMAQQLPEPLASQFAAMNRGDVTRDPVKLGERYYLFKLSEVGKNPDA
                     QPFELVRNQLEQGLRQEKARLKIDAILEENGVKP"
     gene            18233..18724
                     /gene="dtd"
                     /locus_tag="ACFM8S_000459"
     CDS             18233..18724
                     /gene="dtd"
                     /locus_tag="ACFM8S_000459"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFC6313583.1"
                     /translation="MRAVIQKTVGAKVDVVSENGTETCGKIDGGFVVLLGVTHDDTEK
                     DARYIADKIVHLRVFEDEAGKLNLSLKDVGGAVLLVSQFTLYADAASGRRPSFSQAAP
                     AEQAQQLYLRTAELLRGHGIHVETGRFRTHMQVSLCNDGPVTILLDSFMTRISPKMKV
                     VPD"
     gene            18755..19765
                     /gene="dusA"
                     /locus_tag="ACFM8S_000460"
     CDS             18755..19765
                     /gene="dusA"
                     /locus_tag="ACFM8S_000460"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFC6313584.1"
                     /translation="MNDNTHTLPPRHLSVAPMLDWTDRHYRYMARQITRNAWLYSEMV
                     NAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKAAKAAEAYGYNEVNLNCGCPS
                     PRVQKGSFGACLMNEVGLVADCLNAMQDAVGITVTVKHRIGVDRQTEYQTVADFVGTL
                     RDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPGLEIIINGGITTN
                     EAIAGHLQHVDGVMVGREAYHNPMVMREWDRLFYGDTANPIGYADLVQRLYTYSQAQI
                     QAGRGTILRHIVRHSLGLMHGLKGARTWRRMLSDATLLKDNDGSLILEAWKEVERANM
                     RE"
     gene            complement(19835..20722)
                     /gene="gluQRS"
                     /locus_tag="ACFM8S_000461"
     CDS             complement(19835..20722)
                     /gene="gluQRS"
                     /locus_tag="ACFM8S_000461"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFC6313585.1"
                     /translation="MYTGRFAPSPTGLLHIGSLLTAAASYADARSNGGKWLVRMEDLD
                     PPREMPGAASHILHTLEAFGFEWDGEVAYQSRRYALYEETLCRLKTAGLVYPCHCSRK
                     DWQAGARRGADGFVYNGRCRHPGQRPAPQGKQPAWRIRVPDRVIGFSDGIVGGYAQNL
                     AGDIGDFVLLRADGYWAYQLAVVADDAEQSVTHIVRGQDLLVSTPRQIYLQQCLGVPT
                     PQYAHLPLLTNAQGQKWSKQTLAPALDLNRREQLLRQVFRYLKLPEAPETDRPAELLD
                     WAVAHWDMGKVPKHAITAP"
     gene            complement(20739..21029)
                     /locus_tag="ACFM8S_000462"
     CDS             complement(20739..21029)
                     /locus_tag="ACFM8S_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313586.1"
                     /translation="MAKALEIISPNEIYSDLIFKDPVPPHTIYTELMKLIGRNASNER
                     IADRAFDFFSSGLMDGSATEEQYNALYDLTLLEEPGMELNKDEIMALINSLK"
     gene            complement(21034..21168)
                     /locus_tag="ACFM8S_000463"
     CDS             complement(21034..21168)
                     /locus_tag="ACFM8S_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313587.1"
                     /translation="MPSERLSDGIARLTGHSAAYQGFTLKILINKKLMHDITHQHKRK
                     "
     gene            complement(21183..22238)
                     /gene="tal"
                     /locus_tag="ACFM8S_000464"
     CDS             complement(21183..22238)
                     /gene="tal"
                     /locus_tag="ACFM8S_000464"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFC6313588.1"
                     /translation="MTILSDVKALGQQIWLDNLSRSLVQSGELAQMLKQGVCGVTSNP
                     AIFQKAFAGDALYADEVAALKQQDLTPKQRYETMAVADVRAACGVCLAEHESTGGKTG
                     FVSLEVSPELSKDAQGTVEEARRLYAAIGCKNAMIKVPATDAGIDALETLVSDGISVN
                     LTLLFSRAQTLKAYAAYARGIAKRLAAGQSVAHIHVVASFFISRVDGALDTTLPDHLK
                     GKIAIALAKTAYQDWEQYFTAPKFAALETKGANRVQLLWASTGVKNPAYPDTLYVDSL
                     IGAHTVNTVPDATLKAFIDHGTAKATLTEGVEEAQAQLAETAALGIDVETLAARLQED
                     GLKQFEEAFEKLLAPLV"
     gene            22335..>23300
                     /locus_tag="ACFM8S_000465"
     CDS             22335..>23300
                     /locus_tag="ACFM8S_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HFC6313589.1"
                     /translation="MAENGKYLDWAREVLHTEAEGLREIAADLDENFALAADALLHCK
                     GRVVITGMGKSGHIGRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDNDVVVAISNSG
                     ESDEIAAIIPALKRKDITLVCITARPDSTMARHADIHITASVSKEACPLGLAPTTSTT
                     AVMALGDALAVVLLRARAFTPDDFALSHPAGSLGKRLLLRVADIMHKGGGLPAVRLST
                     PLKEAIVSMSEKGLGMLAVTDGQGRLKGVFTDGDLRRLFQECDNFTGLSIDEVMHTHP
                     KTIAAERLATEALKVMQANHVNGLLVTDADGVLIGALNMHDLLAAR"
BASE COUNT         5219 a         5954 c         6678 g         5449 t
ORIGIN      
        1 gctgtacagg tataacaatg aataaatttg gggataaggt cgtatgagcg taggtttgct
       61 gaggattctg gttcaaaacc aggtggttac tgttgagcag gccgagcatt actacaagga
      121 gtcgcaggcg ggtaaggaag tgttgccgat gctgttttca gacggcgtca tttcgcccaa
      181 gtcgcttgcg gcattgattg caagggtgtt cagttattcg attcttgatt tgcgtcatta
      241 tccgcgccac agggtgctga tgggggtgtt gacggaggag cagatggtgg agttccactg
      301 tgtgccggtt ttccgtcggg gcgacaaagt attttttgcg gtttccgatc cgacacagat
      361 gccgcaaatt cagaaaaccg tttctgccgc agggattgag gttgaattgg tcattgtcga
      421 ggatgaccag ttggcgggtt tgctcgattg ggtgggttcg cgttcgacat cgctgcttca
      481 ggagcttggg gaggggcagg aggaagagga aagccacacc ctgtatatcg acaacgagga
      541 ggcagaagac ggccctgttc cgaggtttat ccataagact ttgtcggatg ccttgcgcag
      601 cggggcatcc gacatccatt tcgagtttta cgaacacaat gcccgtatcc gtttccgtgt
      661 ggacgggcag ctccgcgagg tggttcagcc gcccattgcg gtaagggggc agcttgcttc
      721 ccggattaag gtaatgtcgc gtttggacat ttccgaaaaa cggataccgc aggacggcag
      781 gatgcagctg acctttcaaa agggcggcaa gcctgtcgat ttccgtgtca gcacattgcc
      841 gacgctgttt ggcgaaaagg tcgtgatgcg gattttgaat tccgatgccg cgtctttgaa
      901 catcgaccag ctcggttttg agccgtttca gaaaaaattg ttgttggaag cgattcaccg
      961 tccctacggg atggtgctgg taaccggtcc gacgggttcg ggtaagacgg tgtcgctcta
     1021 tacctgtttg aatattttga atacggagtc ggtaaatatt gcaacggcgg aagaccctgc
     1081 cgagattaac ctgccgggca tcaatcaggt taacgtcaat gataagcagg gcctgacttt
     1141 tgccgctgct ttgaagtctt tcctgcgtca ggacccggac atcattatgg tcggtgagat
     1201 tcgtgatttg gaaactgccg atattgcgat taaggcggca caaacagggc atatggtgtt
     1261 ttccaccctg cacaccaata atgcgccggc gacgttgtcg cgtatgctga atatgggtgt
     1321 cgcgccgttt aatattgcca gttcggtcag cctgattatg gcgcagcgtc ttttacgcag
     1381 gctgtgttcg agctgcaaac aggaagtgga acgcccgtct gcctctgctt tgaaggaagt
     1441 cggcttcacc gatgaggacc ttgcaaaaga ttggaaactt taccgcgccg tcggttgcga
     1501 ccgttgccgg gggcagggtt ataaggggcg tgcgggcgtg tatgaggtta tgcccatcag
     1561 cgaagaaatg cagcgtgtga ttatgaacaa cggtacggaa gtggatattt tggacgttgc
     1621 ctataaggag ggtatggtgg atttgcgccg ggccggtatt ttgaaagtta tgcagggcat
     1681 tacttcattg gaagaggtaa cggcaaatac caacgattag gtttgagaat gaaaatgccg
     1741 tctgaagcgt gtttgtttca gacggcattt gactttcagg gtgtttgccg ggaaggcggg
     1801 gcggtcagcg gtatgccatg tcgggttcgg atatttccgg caaactttcc gtttggccgg
     1861 aaaccgtata tttcccgtct gcccatccgc ccaagtcgat cagtttgcag cgttgcgaac
     1921 agaaggggcg gaatgcgttt tcgggttccc atactactgc tgtttgacag gtcggacatt
     1981 tgacttgaag gcgtgtttgc cgcgattcag ccattgtgtt ttccttgtgt tggttttgag
     2041 gcgaaaatcc ctgaataaaa cgcgtgcagg cgcattgttt tctcacgcag gcttttgagg
     2101 ctgccgtcat tgagcagcac atcgtctgca agcagcaggc gttcggattc ggatgcctga
     2161 tggctgatga cggcagccac ctcgccgcgc gtcagcccgc tgcgggccat caccctgccg
     2221 atacgttttt ccaaaggtgc gcttatggtc aggacacgcc gtatcaggct gataaattga
     2281 cgcttttccg tcagcagcgg gatttcgaca atgccgtaag ccgcatcggc aaaggtttct
     2341 tgctgttttt tgatttctga gaaaatcagc ggcaacatca cggattcgag caaggctttt
     2401 cgcgatgggg aggcaaagat ttctttacgc aatatgtcgc gccgcaacaa accctgtgtg
     2461 tcgaaaacgg tgtcgccgaa cagccgcctg atttccggca gggcgatgcc gtctgaagcc
     2521 gtcagcgagt gcgccgtcgc gtccgcatcg atgcgcggca cgcccaaatc ggcaaaacat
     2581 tgcgcggctg ccgatttgcc gctgccgatt ccgccggtca gcccgaccca taccgtcatc
     2641 ttacagcacc ggatgggtca gccaccagtt gaccgcccgc catacggaat cgtttgccgt
     2701 aaaaattatc cagcccgaaa ctgtcagtgc ggggccgaag gcaaaatgct gccccttggc
     2761 gacgcgcata acgattgccg cgaccaaacc gatcagcgag gaaacaaaaa tcagtacggg
     2821 caatgcggat atgccgagcc acgcgcccaa tgcggcaatc agtttgaaat ctccgttgcc
     2881 cataccggtt tttcctgtga gcagtttata cactgcacat aagagccata atgaaccata
     2941 gccggcgacc gcacctaaaa cggcagactg caaaggcacg aagccgccgt ccaaattaaa
     3001 tatcagaccc agccaaatta agggcagtgt catcgagtcg ggcaggtatt gggtgtccgc
     3061 atcgataaag gtcagggaaa tcagaaacgc ggtcagtacc aatccgccca gcgtaatcca
     3121 agaccagccg tattgccagg cgaccagccc gaacaatacg ccggtcagca gctcgattaa
     3181 gggataacgt atgctgattt tggtttggca ggaagcgcat ttgccgcgca ggagcaggta
     3241 gctgacaatc gggatgttct gccacgcgcg tatcggcacg cgacatttgg gacagcagga
     3301 atccggcttc atcaggttga aggtacggct ttcctcttcg gtcagcggca ggtttaaata
     3361 ttctttggca aataccgtcc agccgcgttc catcatgacc ggcacgcggt aaatgacgac
     3421 gtttaagaaa ctgccgacca gcagcccgaa caccgctgcc aaaggcacgg caaacggcga
     3481 caatacagac aaatcagaca tattttgttc tcaatgtatt caaaacaaaa acaaaccggc
     3541 gcagagcgaa tccgcgccgg atctgtgcgg caaatcaggc gaccacgttg cccaaattaa
     3601 acagcggcag atacatggcg accagaagcg tgccgatgac caagcctaaa atcacgataa
     3661 tgatcggctc catcatagcg gacagcctgc cgaccgcatt gtccacctcg tcttcgtaaa
     3721 attcggcggc tttgttgagc atatcgtcca aagaacccga ttcctcgccg atggaagaca
     3781 tctgcaacat catattgggg aacagttccg tcgcacgcat ccccgaagtc atagacaaac
     3841 cttggatgac gcgcgtacgg atttcccggg tggcttcttc atagattaaa ttgcccgccg
     3901 cgccggcagt ggagtccaat acatcgacca aaggcacgcc tgccgcaatc agcgtcgccg
     3961 tcgtcctgcc ccagcgggca atcgtccctt tgcggacaat gtctccgaaa atcggcatac
     4021 gcagcagtat ggcatccata cgccgttgga ttttaatcga acgcgccttc aatttaagga
     4081 agccgtatat ggcaaagccc agtgcgatca gcaccatcca gccgtatgag acgaaaaagt
     4141 cggacatatc catcactgtt tgggtcagtg cgggaagctc cgcgcccata ttggcgtaaa
     4201 cttctttaaa ggcgggcagt acgaaaatca tcatcacgaa taccaaaccg atggcgacgg
     4261 cgatgacgga taccggatag gtcagtgcgg tttttacctt tttgcggata gcctgggttt
     4321 tttctttgta aattgccaat ttgtccagca ggctttccaa tacgccgccc gtttcgcctg
     4381 ccgcaaccag gttgcagtag aagcggtcga aatatttcgg gtggtttgag aatgcgcggc
     4441 tcaacgagct gccctgttcc acttcgcctc ggatttccat cagcatttcc gtcatagacg
     4501 ggttgccgtg tccgcgcgcc acgatttcaa atgcctgcat cagcggcagg cccgctttaa
     4561 tcatcgtgga cagctggcgg gtgaaaacgg tgatgtcttc ttgtgtgatt ttgcgcttgg
     4621 agcttgtttt cacacgggta atctgcaacg ggcggatgcc gcgttttgcc agttttttgc
     4681 gcgcctcttc ttcggtaaac gcggatactt cgccgttgac cagtttgtcg gaggcggaat
     4741 gcctgccttc aaagataaag cgtttttctt tctttgcgaa caaagaaaat cctccgtttt
     4801 tagccatatt ctagccccgt aaagtaattg gaataaaatg taagaaacat cgttaaaaaa
     4861 cagtaccggc gtgttcccgg taagatgaaa accgccgaca tcccgcctgc gggcggcaaa
     4921 cgggacagaa tcggatgcga ttatacctta tttaggcggc tgtccggcat ttatgcgtac
     4981 acaataaatc ttgcaggata ttgttgcggg tcaaatgccg gccggagggt atagtgaatt
     5041 aacaaaaacc ggtacagcgt tggctcgcct tagctcaaga gagaacgatt ctctaaggtg
     5101 ctgaagcacc aagtgaatcg gttccgtact atttgtactg tttgcggctc gccgccttgt
     5161 cctgattttt gttaattcac tatattttcg ccatatggaa ataaggtgct attggacgcg
     5221 gcgggcggtg ttccggagat tcgccaaagc cgctgccgtt tgttaaacta cattctgcta
     5281 cattttaatc cggttctgaa aaatcaagga aaacagatga atgcttttac ccgtgcatgg
     5341 tatgcgctcg aacgccacaa tcaggatacg cgtcatgtcc ttttgcgcga ccgctttgcc
     5401 tgcgaacccg accgttttga gcgtatgcat gagcgtttgg acgggatgtt gttcgattac
     5461 agcaaaaacc gtttgggcga agatacgctg caactgctct gccgtcttgc ggagacggcg
     5521 gatttggaag ggaaaatgcg tgctttgcgg acgggtgcga aagtcaacgg cagcgagggg
     5581 cgtgccgcgc tgcatacggc tttgcgccta cccgacggtg cggatgccgt ttatgcggac
     5641 ggcagggacg tgttgcccga aatccgccgc gagttaaatc gtgcgttgaa gtttgcacac
     5701 agtttggacg acggttcgta tcaggggaca accggaaaac ggattacgga ttttgtccac
     5761 atcggcatag gcggatccga cctcgggccg gcaatgtgcg tgcaggcact cgagccgttc
     5821 agacggcata tcgccgtcca ttttgccgcc aacgccgacc ctgcctgcct ggatgcggtc
     5881 ttatgccgtc tgaaccccga aacgacagtg ttttgcgttg ccagcaagtc cttcaaaaca
     5941 ccggaaaccc tgctcaatgc acaggcagtc aaggcgtggt atcgcggtgc ggggttctcg
     6001 gaatccgaaa cggcgtgcca tttttgcgcg gtgtctgccg acactgcggc agctgcggct
     6061 tttggtatcg cggcggaacg cgtgtttgcg atgtacgact gggtgggcgg acgctattcc
     6121 gtctggtcgc ccgtcggttt gcccgtgatg gttgcggtcg gcggggcgcg tttccgcgag
     6181 ttgttggcgg gggcgcacgc gatggacagg cattttttca gtacgccgac gcgtcataat
     6241 atccccgttt taatggcact gattgccgtg tggtataaca atttccagca ggcggacggg
     6301 cagaccgccg ttccgtacag ccacaacctg cgcctgctgc cggcgtggct gaaccagctc
     6361 gatatggaga gtttgggcaa aagccgcgct tcagacggca gtcccgccgc gtgcaaaacg
     6421 ggcggcatcg tgttcggcgg tgaaggggtc aactgccagc acgcctattt ccaactgctc
     6481 caccaaggca cacgcctgat tccctgcgac tttatcgtcc cgatgacggc gcagggcgtg
     6541 gaggacggac gcagccgttt taccgttgcc aacgcctttg cccaagcgga agccttgatg
     6601 aagggcaaaa ccttggacga agcacgcgcc gaactggcag atttgcccga agcggaacgc
     6661 gaacgcctcg cgccgcacaa agagttcccc ggcaaccgcc ccagcaacag catcctgctc
     6721 gaccgcctca cgccctacaa tctgggtatg ctgatggcgg cttacgaaca caaaaccttt
     6781 gtgcagggtg taatttggga catcaacccc ttcgatcagt ggggggtgga atacggcaaa
     6841 cagttggcga aaaccatcat cggcgaactg gaaggcggca cgtccgtaca cgatgcctcg
     6901 accgaagggc tgatggcgtt ttaccgcgaa tgccgtctga aaggcggcgg cgcggcataa
     6961 aggcaatacc tgccgcctgc ccgattccga aacgccaatg ttcggcaacc gcccgcgtat
     7021 tgctgacgaa tatgcgtttg cgtggcacaa tagcgcattc atttcaaatg aacatactgc
     7081 ctgaaaatac cggcaagcgt cccacgaaac atctcacata aggaaacatt atgtctttgc
     7141 aaaacattat cgaaaccgcc tttgaaaacc gcgcggacat taatccgacc accgttactc
     7201 ccgaagttaa agaagccgtg ttggaaacca tccgccaact cgattccggc aaactgcgcg
     7261 tcgccgaacg cttgggcgtg ggcgaatgga aagttaacga atgggcgaaa aaagccgtgt
     7321 tgctgtcctt acgcatccaa gacaacgaaa tcctcaacga cggtgtgaac aaatacttcg
     7381 acaaagtgcc gaccaagttt gccgactggt ctgaagacga gttcaaaaac gcaggcttcc
     7441 gcgccgttcc gggtgcggtt gcccgacgcg gcagctttgt ggcgaaaaat gtcgtgctga
     7501 tgccgtctta cgtcaacatc ggcgcatacg ttgacgaagg tgcgatggtc gatacttggg
     7561 cgaccgtcgg ctcttgcgcg caaatcggca aaaacgtcca cttgagcggc ggtgtcggca
     7621 tcggtggcgt actcgaaccc ctgcaagcca gcccgaccat tattgaagac aattgcttca
     7681 tcggtgcgcg ttccgaaatc gttgagggcg tgattgtcga agaaggcagc gtgatttcta
     7741 tgggcgtgtt catcggtcaa tccaccaaaa tcttcgaccg caccaccggc gaaatctacc
     7801 aaggccgcgt accggccggt tcggtcgtcg tgtccggcag tatgccttcc aaagacggca
     7861 gccacagcct ttactgcgcc gtcatcgtca aacgcgtgga cgcgcaaacc cgtgcgaaaa
     7921 ccagcgtgaa cgaattgttg cgcggcatct gatgccttaa accgtatttg aaacgtccga
     7981 tgccgtctga aatccgcttc ggacggcatt gccgtttgca tgctgcaacg tgaaaacaca
     8041 gaaacaggga caatttgcta taatcaacgg tttagaatga accgaacact atttgaagga
     8101 tacaaaatgg gttttctgca aggcaaaaaa attctgatta ccggcatgat ttccgagcgt
     8161 tccatcgctt atggcatcgc caaagcctgc cgcgaacaag gcgcggaact ggcgtttacc
     8221 tacgttgtgg acaaactgga agagcgcgtc cgcaaaatgg cggcggaatt gggttccgaa
     8281 ctcgtattcc gctgcgatgt cgccagcgac gacgaaatca accaagtgtt cgccgacttg
     8341 ggcaaacatt gggacggctt ggacggtttg gtgcattcca tcggcttcgc gccgaaagaa
     8401 gccttgagcg gcgacttcct cgacagcatc agccgcgaag cgttcaacac cgcacacgaa
     8461 atttccgcat acagcctgcc cgcattggca aaagccgccc gtccgatgat gcgcggcaga
     8521 aactccgcca tcgttgccct gagctacttg ggcgcggtgc gcgcgattcc aaattacaac
     8581 gtgatgggta tggcaaaagc cagccttgag gcgggcatcc gttttaccgc cgcctgtctg
     8641 ggcaaagaag gcatccgctg caacggcatt tccgccggcc cgattaaaac gcttgccgcc
     8701 tccggtatcg ccgatttcgg caaacttttg ggacacgtcg ccgcccacaa tccgctgcgc
     8761 cgcaacgtta ccattgaaga agtcggcaat accgccgcct tcctgctgtc cgacctgtcg
     8821 tccggcatta ccggcgaaat cacttacgtt gacggcggtt acagcattaa tgccttgagc
     8881 accgagggat aatccgccgt tttcaaatcc gtgcgccgtc cgtaccgcgt atcggtttcg
     8941 ggcggcggtt tgccgtctga agcgtatttc tagggaaatg cccgacttac ggcaggtgca
     9001 gcggaaatgg tccgaacgcg aaaatgccgt ctgaaacgcc aaacgggttt cagacggcat
     9061 ttttttattt aaagcatcag cacacttcaa ccagccagcc gtatttgtct tccgccaaac
     9121 catactggat gtcggtaatc gccttacgga tggcatagcc gcgttcttgg cttttcactt
     9181 cgatttcttt gccgccgatg acgaaggaag taacgggcga gatgacggct gccgtaccgg
     9241 tcagaatcgc ctccgcaccg ttttccaccg cagctttgag ttcgtcaacc gtgaaattgc
     9301 gttcgctgac ggtatagccc aaatctttgg caaccgtcag tacggaatcg cgggttacgc
     9361 cgtgcaaaaa ttcgtcggtc agcggtttgg taatgatttc atcgccgtta atcaggataa
     9421 agttggacgc gccggtttcc tgcacgtcgc cgttcgggca gaagaggact tggtttgcgc
     9481 cgtattcggc tttcgccttc agcacccagt gcatggcgga agcgtagttg ccgccgcatt
     9541 tgacgcggcc catatgcggg gcgcagcgga tgtgttcggt ttccactaaa attttgacgg
     9601 gcgagccgac tttgaaatag tcgccgacgg gggaagccaa aatatacagc agggcggttt
     9661 cggaaggaga accggccttg ccgatgacgg gatcggtacc gattaaggtc gggcgcaggt
     9721 acagggcggc aggcgcatcg ggaatttcgt cggcggcacg tttgaccagt tcgaccaaag
     9781 catcgagata ggcttgggtt tcggggcggg gcaggtgcaa aatgtccgca ctttgccgca
     9841 tacgcgcgat attggcagtc ggacggaaca gtacgatttt accgtctgcc tgacggaagg
     9901 ctttcagtcc ctcgaaacat tcgctgccgt agtgcagggc gtgcgcaccc ggtacgaggg
     9961 agaggtcttg ggaagattgc cattcggtcg gctgccattt gccttcgcgg taggcgagga
    10021 cgggcatttg actgtgaaaa acgctgccga atacggcggg tacgggtctg ctcatgatgt
    10081 aaagcctttc ttattctgat atgtttcaat gaacggtttg aatttgaaga ttgtaaagat
    10141 acgcctgcaa acagggtttt gacaagtgcg cggcgggttt ttctgtcgat gcggtgtcca
    10201 atccgttatt tttcaaatgg aaaggaacgg tgtatttggt aaaattgtcg gcaatcgcat
    10261 actccgtatg tcgtccgaac acgctgccgc atcctatccg aaaccgtgca aatcgtttaa
    10321 actagcgcaa tcttggttca gagtgcgaag ctgtccgggc ggcgttttta tttacggagc
    10381 aaacatgaaa cttatctata ccgtcatcaa aatcattatc ctgctgctct tcctgctgct
    10441 tgccgtcatt aatacggatg ccgttacctt ttcctacctg ccggggcaaa aattcgattt
    10501 gccgctgatt gtcgtattgt tcggcgcatt tgtagtcggt attgtttttg gaatgtttgc
    10561 cttgttcgga cggttgttgt cgttacgtgg cgagaacggc aggttgcgtg ccgaagtaaa
    10621 gaaaaatgcg cgtttgacgg ggaaagagct gaccgcacca ccggcgcaaa atgcgcccga
    10681 atctgccaaa cagccttgag aaagccgata tggacaatga attgtggatt atcctgctgc
    10741 cgattatcct tttgcccgtt ttcttcgcga tgggctggtt tgccgcccgt gtggatatga
    10801 agactgtatt aaagcaggca aaaagcatac cgtcgggatt ttataaaagt ctggatgcct
    10861 tggttgaccg caacagtgga cgtgctgcca gggaattggc agaggtcatc gatcagcagc
    10921 ctcagtcata cgacctgaac ctcaccctag gcaagcttta ccgtcagcgt ggcgaaaacg
    10981 acaaagccat caatatgcac caaacattgc ttgactctcc cgatacaacc ggagccaagc
    11041 gtgcgcgcgt gttgtttgaa ttggcgcaaa actatcaaag tgcggggttg gttgatcgtg
    11101 ccgaacagat tttcctcagt ctgcaagacg gtgaaatggc gcgtgaagcc agacagcacc
    11161 tgctcaatat ctaccagcag gacagggatt gggaaaaagc gattgaaacc gcccaactcc
    11221 tcagtcacga cgaacagacc tatcagtttg aaattgccca gttttattgc gaacttgccc
    11281 aagccgcgct gttcaagtcc aatttcgatg tcgcgcgttt caatgtcggc aaggcactcg
    11341 aagccaacaa aaaatgcacc cgcgccaaca tgattttagg cgacattgaa tacaggcagg
    11401 gcaattttcc tgcatcggtt gaagcgtatg ccgccatcga gcagcaaaat catgcctatt
    11461 tgagcatggt cggagagaaa ctttatgacg cttatgcggc gcaaggaaag cctgaagaag
    11521 gattaaatcg cctgataggg tatatgcaga ccttcccgga acttgatttg attaacgtcg
    11581 tgtacgagaa atccttattg ctcagggggg agaaagaggc tgcacaaact gctatcgagc
    11641 ttgttcggcg caaaccggac ttgaacggtg tttaccgcct gctcggttta agaatcagcg
    11701 atatggatcc gacttggaaa gccgatgccg atatgatgcg ttcggttatc ggacggcagc
    11761 tacagcgcag cgtgatgtac cgttgccgca actgccactt caaatcccaa gtctttttct
    11821 ggcactgccc cgcctgcaac aaatggcaga cgtttacccc gaataaaatc gaagtttaac
    11881 caccaccgaa aggaacacaa aaaatgcgct tactccatac tatgctccgc gtgggcaatc
    11941 tcgaaaaatc cctcgatttc taccaaaacg ttttgggtat gaaactgctc cgccgaaaag
    12001 attatcccga aggcagattt acccttgcct tcgtcggtta cggcgatgaa accgacagca
    12061 cggttttgga actgacgcac aactgggata cggaacgata cgacttgggc aacgcctacg
    12121 gacacatcgc ggttgaagtg gacgatgcct acgaagcctg cgaacgtgtg aagcggcagg
    12181 gcggaaacgt cgtccgcgaa gccggcccga tgaaacacgg cacaaccgtg atagccttcg
    12241 tcgaagaccc cgacggatac aaaatcgagt tcattcaaaa gaaaagcggc gacgattcgg
    12301 ttgcctatca aactgcctga taccgccgcc gccaatgccg tctgaagcct ttaggggttt
    12361 cagacggcat tttgttgccg tcggcctgct gtttgagcct gtgccggttc aaactttatc
    12421 cgttacaccg ataaggcaaa aaaagatgcc gtctgaaacg gcatccttga tctgcgaaag
    12481 ggcagttggg aatcaaatac ccaattcctg cgccaatgct tgggcacgtt tgagtacgtc
    12541 gccttccgct tcttccagca atttctgcac tgtctcggca gcggcatcgc ggtcgccgat
    12601 ttcgagatac atttcggcaa ggtcgtattt cgcttcggaa ggcgcgtcag aacctacaga
    12661 ttccgaaggg aaactggtat ctgcattatt tgggatattt tcttccgaga ggtagatgct
    12721 ccaatctacc gtttcctcct cgccgtcttt caggaagtcg ggcaaagcgt ctgcctcaga
    12781 ggtgttggaa tcaggcgttt ccaaagtgat ttccgctgca ttttcctcaa cggccggtgc
    12841 ttcagcaggt tgcaacagtg cggacaaatc atcggcaacg gtttccgctg cattttcctc
    12901 aacggcaggt acttcagaag gttgaagtaa tgcggacaaa tcgtctgcgg tggcgttgaa
    12961 atcgggtgtt tcggcaacag tttccgttac attttcctca acagccggtg cttcagcagg
    13021 ttgcaacagt gcggacaaat catcggcaac gatttccgct gcattttcct caacggcagg
    13081 tacttcagaa ggttgaagta atgcggacaa atcgtctgcg gtggcgttga agtcgggtgt
    13141 ttcggcaacg gtttccgtta tattttcctc aacggacggt gcttcggcag gttgaagcaa
    13201 tgcggacaaa tcgtctgcgg cggcgttgaa atcgggcgtt tcaggcgcag tttccgcgac
    13261 ggcatcggtt tcgtacactt tcaggaaatc gtgcaactct tccggtgttt ggacttcggc
    13321 aactgttttt tccaagatgg tttcgggcga ggaagccttc aggaagcctg ccagtccgga
    13381 gggtgaggca ggttttgcgg aagctgtttc ttctgtgccg atatggttgt ttgagggcag
    13441 gttgtcggag aaatcggtat cgacggtttc cggtttgttt tcggcagttt gggcgacaga
    13501 ttccggttcg ggcgtgtcga tgacgatttc gacagggttg tacgggttga aggtctcggg
    13561 ctcgtacacg ctgtctgtgg attcgatggc gttccaatcg gcatccgcgc gtttttgggt
    13621 ttcttcatcc tgcgtaagtg cgccggataa aatgccgttt tgcgcggctg ccaggctgtc
    13681 gaaatccaag tcgatgcggt tggaaggcgt atcggtttcg acatcgaacg tttgttttgc
    13741 cgacaactct tcttcagatt ccccatctaa ggcaagtgtg tcgtttacat cgtttttcgg
    13801 agcgggttcg ggcgttgccg gagtttcgac ttcggcaaag gtgatttcta tgccgtcgtc
    13861 tgccgcgtcg tcaaggtcag gctcttcctc agggacggat tcttcggtac ggcgcgcgcg
    13921 tttggattgg gcaaggcgca aaagcagcag cagggcgatc aatgccgcgc ctccgccggc
    13981 aagcagcaag gtgtacgaac cgccgaacag tccgctttcg gtttcttctt cggcagaaac
    14041 ctgttcgaca ggttcggaaa cggcgttacc ggtttcgtcg gtcggcgtgt cgatggcaga
    14101 agcggcggct tcttgggggg cggattcggc agcggtttcc gatgcggcag tatttgcagc
    14161 gggtacaggt tcgggtcgaa cggccggttt ttccgctttt gcttcgggcg cggcaacttt
    14221 tgcttcaggt ttttcaaccg gtttctctac cgttgcctgt ttggacggtt cggacggcat
    14281 ggatgcggtt tcggctttgg gtttcgccgt ttgcggtttg ggttgttccg ctttgatcct
    14341 gttcagattc ggaatgtgaa gcacgctgcc cgcacgcagt ctgccgtgtg cggaaacatt
    14401 tgggtttgcc ttcagcagcg catcggcaac ctgttcgagc gtcaggtgtt tcggacggat
    14461 ggcggcggca atctgtttga ccgtttcgcc tttgcggacg gtatgggttt tgccgttgta
    14521 cgccggtttg acggctgcgt tcgcgctgtc ttttttatcg gttttgcgga gggctttggc
    14581 gttttgattt tcttgggact ctgctgtcgg agcggttttg cggtgtgtct tgccgtctga
    14641 aagtgcagat ttggttttgg gcgagtagcc gacaggatcg aggatggcgg tgtattcgcg
    14701 tacctgtgcg cctgcgccga tgcggaatac caggacggga tcgcggactg cctgttcgga
    14761 agaaacggca atgacggctt tgtcgcccaa cttgtggact ttggcggtca ggcctttttc
    14821 ggaaacggta acgctgccgc cgcctagcag ggctttggct tcttcgccgg ttacggtaat
    14881 gctgccggaa aagggttcgt caaggttgga ctggatattc agtccgccca gtccagcatg
    14941 tgcctgaaag gatgcggcaa ctgcgacgga ggcggcaatc agtttgattt gtctgttgtt
    15001 tttcaagatg tatcccctgt gggttggcgg ctgaatacgg tttgaccgcg tacagtctgt
    15061 aaatttcgtc atcatcgggc atcggcgggg cagtcggccg gcgggcattt aatatgtgaa
    15121 tgtaccgacc gccgccacat tttaaacggc aatcattcgc cgtttttaca aattatgaca
    15181 tatctccatc ttttttcaaa aacatctgtg catatttgca tcaatcaaaa caaaatttgt
    15241 tggttttgca ggtgcaaaaa cagggttctg cctgtatgat tagcgtttat ttgatttgct
    15301 ttctcatttg gatatgaaat tcgtcagcga ccttttgtcc gtcatcctgt ttttcgccac
    15361 ctataccgtt accaaaaaca tgattgccgc aacggcggtc gcattggttg ccggtgtggt
    15421 tcaggcggct tttctgtatt ggaaatataa aaagctggat acgatgcagt gggtcggatt
    15481 ggtgctgatt gtggtattcg gcggcgcaac cattgttttg ggcgacagcc gcttcattat
    15541 gtggaagccg agcgttttgt tttggctggg cgcgctgttc ctgtggggca gccacctcgc
    15601 cggtaaaaac ggcttgaagg cgagtatcgg cagggagatt cagctttcag atgccgtatg
    15661 ggcgaaattg acgtatatgt gggtcggttt tctgattttt atgggtattg ccaactggtt
    15721 tgtgtttact aggtttgaag cgcaatgggt caactataaa atgttcggct cgactgcact
    15781 gatgcttgtt ttctttatta ttcagggtat ttatctgagt acctgtctga aaaaggagga
    15841 ttgactgtgg aatattttat gttgctggca acagacgggg agaatgtgta tgaagcgcgt
    15901 atggcggcac gtcccgaaca cctcaaacgg ctggagacgc tgaagtcgga aggccggctg
    15961 ttgacggcag gcccgaatcc tttgccggag gactccaacc gcgtttcggg cagtttgatt
    16021 gtggcgcagt tcgagtcttt ggatgcggca gaggcgtggg cggaagacga tccctatgtt
    16081 catgcaggcg tgtacagcga agtgctgatc aagccgttta aagcggtgtt caaataatgc
    16141 cggccgtcga tttgatccgc gaacgcctgc agacgctcga tccgctggtg ttggaaatcg
    16201 gcgatgagag ccatctgcac aaaggacacg cgggcaatac cggcggcgga cattatgccg
    16261 ttttggtcgt cagcggccgt tttgaaggcg taagccgcct gaaccgccag aaaacggtca
    16321 aatcgctgct caaagatttg ttttcaggcg gcatgattca cgcgctcggc atccgggcgg
    16381 ctacccctga cgagtatttc catacggcgg actgaatgaa gtctgcccga acatttcaat
    16441 ttaaaattta aagagagaag attatgaaag caaaaatcct gacttccgtt gcactgcttg
    16501 cctgttccgg cagcctgttt gcccaaacgc tggcaaccgt caacggtcag aaaatcgaca
    16561 gttccgtcat cgatgcgcag gttgccgcat tccgtgcgga aaacagccgt gccgaagaca
    16621 cgccgcaact gcgccaatcc ctgctggaaa acgaagtggt caacaccgtg gtcgcacagg
    16681 aagtgaaacg tctgaaactc gaccggtcgg cagagtttaa aaatgcgctt gccaaattgc
    16741 gtgccgaagc gaaaaagtcg ggcgacgaca agaaaccgtc cttcaaaacc gtttggcagg
    16801 cggtaaaata tggcttgaac ggcgaggcat acgcgctgca tatcgccaaa acccaaccgg
    16861 tttccgagca ggaagtaaaa gccgcatatg acaatatcag cggtttttac aaaggtacgc
    16921 aggaagtcca gttgggcgaa atcctgaccg acaaggaaga aaatgcaaaa aaagcggttg
    16981 ccgacttgaa ggcgaaaaaa ggtttcgatg ccgtcttgaa acaatattcc ctcaacgacc
    17041 gtaccaaaca gaccggcgcg ccggtcggat atgtgccgct gaaagatttg gaacagggtg
    17101 ttccgccgct ttatcaggca attaaggact tgaaaaaagg cgaatttacg gcaacgccgc
    17161 tgaaaaacgg cgatttctac ggcgtttatt atgtcaacga cagccgcgag gtgaaagtgc
    17221 cttcttttga tgaaatgaaa ggacagattg ccggcaacct tcaggcggaa cggattgacc
    17281 gtgccgtcgg tgcactgttg ggcaaggcaa acatcaaacc tgcaaaataa ttctgaaaac
    17341 gggatatggc ggcaagacgt tcagacaggc gttttgccgc cgcgcaggac agggaatacc
    17401 atgaaacaga aaaaaaccgc tgccacagtt attgctgcaa tgttggcagg ttttgcggca
    17461 gccaaagcac ccgaaatcga cccggctttg gtggatacgc tggtggcgca gatcatgcag
    17521 caggcagacc ggcatgcgga gcagtcccaa aaaccggacg ggcaggcaat ccgaaacgat
    17581 gccgtccgtc ggctgcaaac tttggaagtt ttgaaaaaca gggcattgaa ggaaggtttg
    17641 gataaggata aggatgtcca aaaccgcttt aaaatcgccg aagcgtcttt ttatgccgag
    17701 gagtacgtcc gttttctgga acgttcggaa acggtttccg aaagcgcact gcgtcagttt
    17761 tatgagcggc aaatccgcat gatcaaattg cagcaggtca gcttcgcaac cgaagaggag
    17821 gcgcgtcagg cgcagcagct cctgctcaaa gggctgtctt ttgaagggct gatgaagcgt
    17881 tatccgaacg acgagcaggc ttttgacggt ttcattatgg cgcagcagct tcccgagccg
    17941 ctggcttcgc agtttgccgc gatgaatcgg ggcgacgtta cccgcgatcc ggtcaaattg
    18001 ggcgaacgct attatctgtt caaactcagc gaggtcggga aaaaccccga cgcgcagcct
    18061 ttcgagttgg tcagaaacca gttggaacaa ggtttgagac aggaaaaagc ccgcttgaaa
    18121 atcgatgcca ttttggaaga aaacggtgtc aaaccgtaat ggcatttcca ataccgatgc
    18181 cgtctgaagc cttttcagac ggcattgcac gttcaggtaa ggaggacggc ttatgcgtgc
    18241 ggtcatacag aaaacggtag gtgcaaaggt ggatgtcgtg tccgaaaacg gcacggaaac
    18301 ctgtggcaaa atcgacggcg gattcgtcgt gctgctcggc gtaacgcacg acgacacaga
    18361 aaaagatgcg cgctatatcg ccgacaaaat cgtccatttg cgcgtgtttg aagacgaagc
    18421 gggcaagttg aacctgtctt tgaaagatgt cggcggcgcg gtgctgctgg tgtcgcagtt
    18481 tacactttat gccgatgcgg caagcgggcg gcggccttcg ttttcccaag ccgcacctgc
    18541 agaacaggcg cagcagcttt acctgcgaac ggcggaactg ttgcgcggac acgggattca
    18601 tgtcgaaaca gggcgtttcc gcacgcatat gcaggtgtcg ctctgcaacg atgggccggt
    18661 aaccatactg ctggactctt tcatgacgcg gatttcccca aaaatgaagg ttgttccgga
    18721 ttgaaattga atccgcaatg gtgaaatatc gacaatgaac gacaatacac acacccttcc
    18781 cccgcgccac ctgtccgtcg cccccatgct cgactggacg gacaggcact accgttatat
    18841 ggcgcgccag attacccgaa atgcttggtt gtacagcgaa atggtcaacg ccggcgcgat
    18901 tgtttacggc gacaaagacc gctttttgat gttcaatgaa ggcgagcagc ccgtcgccct
    18961 gcaactgggc ggcagcgatc cgtccgattt ggcgaaagcc gccaaagccg ccgaagcata
    19021 cggctacaac gaggtcaacc tcaactgcgg ctgtcccagt ccgcgcgtcc agaaaggttc
    19081 gttcggcgcg tgtctgatga acgaagtcgg gctggttgcc gactgcctca acgccatgca
    19141 ggacgcggtc ggcattaccg ttaccgtcaa acatcgcatc ggtgtggaca ggcagaccga
    19201 ataccaaacc gttgccgatt tcgtcggcac gctgcgcgac aaaaccgcct gcaaaacctt
    19261 tatcgtccac gcccgcaacg cttggctgga cggtctttca cccaaagaaa accgcgacgt
    19321 tccgcccttg aaatacgatt atgtttaccg cctcaagcag gagtttcccg ggctggaaat
    19381 catcatcaac ggcggcatca ccaccaacga agcaatcgcc ggacacctgc aacacgttga
    19441 cggcgtgatg gtcgggcgcg aggcgtacca caacccgatg gtgatgcgcg aatgggacag
    19501 attgttttac ggcgacactg cgaacccgat cggatacgcc gatttggtgc aacgcctcta
    19561 cacatacagc caagcccaaa tccaagccgg acgcggcaca atcctgcgcc atatcgtccg
    19621 ccacagcctc gggctgatgc acggcttgaa aggcgcgcgg acttggcggc gtatgctttc
    19681 cgacgcaacg ctcttgaaag acaacgacgg cagcctgatt ctcgaagcgt ggaaagaagt
    19741 cgaacgggca aatatgcgcg aatagggcgg ggctgtgtgt atgaaatgcc gtctgaaggt
    19801 ttcagacggc atttgtgcgt ttgtcgggcg gtgtttaggg ggcggtaatg gcgtgtttcg
    19861 gcactttgcc catatcccag tgtgccaccg cccagtcgag cagttcggca gggcggtcgg
    19921 tttccggtgc ttcgggcagc ttgaggtaac ggaacacttg gcggaggagt tgttcgcggc
    19981 ggtttaaatc caatgcgggg gcgagcgtct gtttcgacca tttctgccct tgcgcgttgg
    20041 tcagcagcgg caggtgggca tattgaggtg ttggaacgcc caaacactgc tgcaaataga
    20101 tttggcgcgg cgtagaaacg agcaggtctt gcccgcggac gatgtgggta acgctctgtt
    20161 cggcatcgtc ggcaacgacg gcgagttggt atgcccagta accgtctgca cgaagcagga
    20221 cgaaatcgcc gatgtcgccg gcgaggtttt gggcgtaacc gccgacgatg ccgtctgaaa
    20281 aaccgataac gcggtcgggg acgcggatgc gccacgccgg ctgtttgcct tgcggtgcag
    20341 ggcgttgacc ggggtggcgg cagcgtccgt tatagacgaa cccgtctgcg ccccgccttg
    20401 ccccggcctg ccagtctttg cggctgcaat ggcagggata gaccagtccg gcggttttca
    20461 ggcggcatag ggtttcttca tacagggcgt aacggcggct ctgataggcg acttctccgt
    20521 cccactcgaa tccgaatgcc tcaagcgtgt gcaggatatg gcttgccgcc cccggcattt
    20581 cgcgcggtgg atcgaggtct tccatgcgga ccagccattt gccgccgttt gagcgtgcat
    20641 cggcatagga agcggcggcg gtcagcagcg agccgatgtg gagcagcccg gtcgggctgg
    20701 gggcgaaacg tcctgtgtac atatccggta cggccccttt atttaagact attaatcaaa
    20761 gccattatct catctttatt cagttccatc ccgggttctt caagtaaggt taaatcatat
    20821 agggcattat attgctcttc ggtagctgaa ccatccataa ggccggacga gaaaaaatcg
    20881 aaggctctat ctgcgattct ctcattactt gcatttctac caatcagttt catcaattct
    20941 gtatatattg tatggggagg taccggatcc ttaaaaatca gatctgaata aatttcattg
    21001 ggtgaaatga tttcgagtgc ttttgccatg attctatttc cttttgtgtt ggtgggtaat
    21061 gtcgtgcatt aacttcttgt tgattaatat ttttagggtg aatccttgat atgccgcact
    21121 gtgtccggtc aaacgggcga tgccgtctga aagcctttca gacggcatcg gggatgaaaa
    21181 gtttaaacca acggtgcgag cagtttttca aacgcttctt caaattgttt caaaccgtct
    21241 tcctgcaaac gcgccgccaa ggtttcgaca tcgatgccga gcgcggcggt ttcagcaagc
    21301 tgcgcttgtg cttcttctac gccttcggtc agcgtggctt tggctgtgcc gtggtcgata
    21361 aaggctttga gtgtggcatc gggaacggtg ttgacggtgt gcgcgccgat caggctgtca
    21421 acgtagagcg tgtcgggata ggctgggttt ttcacgccgg tagatgccca caaaagctgc
    21481 acgcggtttg cgcctttggt ttccagtgcg gcaaatttgg gggcggtgaa atattgttcc
    21541 caatcttgat aggcggtttt ggcaagggcg atggcgattt tgcctttgag gtggtcgggc
    21601 agtgttgtgt ccagcgcgcc gtccacgcgg gagatgaaga agctggcgac gacgtggata
    21661 tgggcaacgc tttgtccagc tgccaagcgt ttggcaatgc cgcgcgcgta ggcggcgtag
    21721 gctttgaggg tttgggcgcg tgagaacagc agcgtgaggt tcacgctgat gccgtccgaa
    21781 acgagggttt cgagcgcatc gatgcctgcg tcggtggcag gcactttaat catcgcgttt
    21841 ttgcacccga tggcggcgta gaggcggcgc gcttcttcaa ccgtgccttg cgcgtctttg
    21901 gacaattcgg gcgaaacttc gaggctgacg aagccggttt tgccgccggt ggattcgtgt
    21961 tcggcaaggc agacaccgca ggcggcacgc acatcggcaa ccgccatcgt ttcgtagcgt
    22021 tgtttggggg tcaggtcttg ctgcttgagg gcggcaacct cgtcggcgta aagcgcgtcg
    22081 ccggcgaagg ctttttgaaa gatggcggga ttggaagtta cgccgcacac gccttgtttc
    22141 agcatttgcg ccaattcgcc gctttgcacg agcgagcggg aaaggttgtc cagccagatt
    22201 tgttgtccta atgctttaac gtccgataaa atagtcattt ctgattcctt tggatggatg
    22261 tgtatgtttc aaaggcttat gctaccccga ttcggaaatt ttgggtagtt ttgttacagc
    22321 aaaggcggat ggcaatggca gaaaacggaa aatatctcga ttgggcgcgc gaagtgttgc
    22381 acaccgaagc ggaaggcttg cgcgaaattg cggcggattt ggacgaaaac ttcgcccttg
    22441 cggcggacgc gttgttgcac tgcaaaggca gggtcgttat cacgggcatg ggcaagtcgg
    22501 gacatatcgg gcgcaaaatg gcggcaacca tggcctcgac cggcacgcct gcgtttttcg
    22561 tccaccctgc ggaagcggca cacggcgatt tgggtatgat tgtggacaac gacgtggtcg
    22621 tcgcgatttc caattccggt gaaagcgatg aaatcgccgc catcattccc gcactcaaac
    22681 gcaaagacat taccctcgtc tgcatcaccg cccgccccga ttcaaccatg gcgcgccatg
    22741 ccgacatcca catcacggcg tcggtttcca aagaagcctg cccgctgggg cttgccccga
    22801 ccaccagcac caccgccgtc atggctttgg gcgatgcgtt ggcggtcgtt ctgttgcgcg
    22861 cgcgcgcgtt cacgcccgac gacttcgcct tgagccaccc tgccggcagc ctcggcaaac
    22921 gcctactttt gcgcgttgcc gacattatgc acaaaggcgg cggcctgcct gccgtccgac
    22981 tcagcacgcc cttgaaagaa gccatcgtca gcatgagtga aaaagggctg ggcatgttgg
    23041 cggtaacgga cgggcaaggc cgtctgaaag gcgtattcac cgacggcgat ttgcgccgcc
    23101 tgtttcaaga atgcgacaat tttaccggtc tttcgataga cgaagtcatg catacacacc
    23161 ctaaaaccat cgccgccgaa cgcctcgcca ccgaagccct gaaagtcatg caggcaaacc
    23221 atgtgaacgg gcttctggtt accgatgcag acggcgtgct gatcggcgcg ctgaatatgc
    23281 acgacctgct ggcggcacgg
//
LOCUS       DAXHSF010000015        22334 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.015, whole genome shotgun sequence.
ACCESSION   DAXHSF010000015 DAXHSF010000000
VERSION     DAXHSF010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22334
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.015"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            1..99
                     /locus_tag="ACFM8S_000466"
                     /pseudo
     CDS             1..99
                     /locus_tag="ACFM8S_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(120..1034)
                     /locus_tag="ACFM8S_000467"
     CDS             complement(120..1034)
                     /locus_tag="ACFM8S_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFC6313590.1"
                     /translation="MDAVQLKSFVAVAHEGNLTQAAKRLFLSQPAVSAQIKALEEYVG
                     TPLFRRTGKGMVLTRAGEILLPEAESLLQYKHKLEYFAKTLAGDYSEETSLGIIHPID
                     SAKLVALTDNIGQTAPKTRLHIQYGMSGEILSRIQHKTLHGGFILGNAAQRGIRSVFL
                     QNLTYALICPQSQYPHLTRSLPQSLQECVWIEMSGVSGSRKHLHQFWRSNRLSPKKQI
                     LCDYPQTIIDLVAGGIGVAMVPGNKAEAAAKEGAGVAIIESCRHSMPLNFIYAEEYED
                     NPHVSLLLECIEKVWGVQAVQPPVVSDN"
     gene            1290..2759
                     /locus_tag="ACFM8S_000468"
     CDS             1290..2759
                     /locus_tag="ACFM8S_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
                     /protein_id="HFC6313591.1"
                     /translation="MEKHNGTYRDLHRPASEFATRDEYLEHELQIMQPKRWRPNLPFR
                     DYRFEWEDLIPAMAGTIGKVVMVGAVAAAFAAPLGLPDSFVLENVRYELLIAAAFILL
                     VSGFFLPGANLPGTHGPLIPMIPIVVSAGGHPLAFGISIAVLGLLMALFRGGSIMAKL
                     TSNGVCGGLLLYLGFIGTTGQVKKLFSWAGGFNMPYIAFTVIIVTIVMYALLEHWKKR
                     WLAVPLGCLIAGVVAFALGAPFEFHTAPGLPPMSPAYWWGENSGWHLGLPTAESFLVV
                     FPFAVLAVAMWSPDFLGHQVFQKISYPEKTDKVLMNIDDTMTSCSVRQAVGSILGGAN
                     FTSSWGTYIVPASIAKRPIPGGAVLTAVLCIIAGLWGYPMDLAIWQPVLSVALVVGVY
                     LPLLEAGMEMTRKGKTTQSAAIVVFSSALVNPVFGWALTMLLDNLGLIGCKERSAQLG
                     FAGRVLIPAVGFLILCVAMGAVGMLPGIPPFLEHFKSLG"
     gene            3011..3382
                     /locus_tag="ACFM8S_000469"
     CDS             3011..3382
                     /locus_tag="ACFM8S_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313592.1"
                     /translation="MNKTLSILPVAILLGGCAAGGGNTFGSLDGGTGMGGSIVKMAVG
                     SQCRAELDKRSEWRLTALAMSAEKQAEWENKICACVAQEAPERMIGNDVMQMLDPSTR
                     NQALAALTAKTVSACFKHLYR"
     gene            3455..4864
                     /gene="thrC"
                     /locus_tag="ACFM8S_000470"
     CDS             3455..4864
                     /gene="thrC"
                     /locus_tag="ACFM8S_000470"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFC6313593.1"
                     /translation="MKYISTRGETAHKPFSEVLLMGLAPDGGLMLPEHYPQIGRETLD
                     KWRGLAYPELAFEIMRLFVTDIPEDDLRDILNRTYTEAAFGTKEITPVRTLSDGIKIQ
                     ALSNGPTLAFKDMAMQFLGNAFEYVLNKEGKKLNILGATSGDTGSAAEYALRGKKGVN
                     VFMLSPDGKMSAFQRAQMYSLQDGNIHNIAVKGMFDDCQDIVKAVQNDAAFKEKYHIG
                     TVNSINWGRIVAQVVYYFAGYFKATQSNDEQVSFCVPSGNFGNVCAGHIAKQMGLPIR
                     RLIVATNENDVLDEFFKTGAYRPRNSAHTYVTSSPSMDISKASNFERFVFDLMDRDPQ
                     EINTLWTEVAAGKGFDLQFALDKVGGKYGFTSSKSTHADRLATIKQVYEQDQELIDPH
                     TADGVKVAREVREEGETVVCLETALAAKFDATIREAVGDAAIPRPAALEGLENLPQRV
                     QTVPNSADAVKGIIEQTLA"
     gene            4910..5704
                     /locus_tag="ACFM8S_000471"
     CDS             4910..5704
                     /locus_tag="ACFM8S_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313594.1"
                     /translation="MTLPMQETRFSILLDELAAKQEAAIAPHLLADGTKVWIRKAGRH
                     NARWRYALLGMVARYLKLGVLKPVPSLGGEPAITTESKRLYELRAAGIAVPELLAVRK
                     NALMFGNLEGIPLDTQIRQEAEAGKSDAWLAGLEAIARVHKKRQFLSQAFARNMMSDG
                     KNISFLDFEDDPSEVLTIAQCQARDWLCYIHSTALILKNGGLLEAAAEKWGGVLSDQP
                     AEIQKLIAATVKPILPIRRLEHPRWGRDALRLAASISLISLADMPP"
     gene            5892..6668
                     /locus_tag="ACFM8S_000472"
     CDS             5892..6668
                     /locus_tag="ACFM8S_000472"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HFC6313595.1"
                     /translation="MAAFNTQKVLSVHHWTDAYFTFTCTRDESLRFENGQFVMVGLMV
                     DGKPLMRAYSVASANWEEHLEFFSIKVQDGPLTSRLQHLKVGDDVLISKKPTGTLVAG
                     DLNPGKHLYLLSTGTGIAPFLSITKDPEIYEQFEKIILVHGVRYKKDLAYYDRFTKEL
                     PEHEYLGDLVKEKLIYYPIVSREEFEHHGRLTDLMVSGKLFEDIGLPKINPQDDRAML
                     CGSPAMLKDTCKVLDDFGLTVSPKTGVRGDYLIERAFVDQ"
     gene            6744..6850
                     /locus_tag="ACFM8S_000473"
                     /pseudo
     CDS             6744..6850
                     /locus_tag="ACFM8S_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(7429..9900)
                     /locus_tag="ACFM8S_000474"
     CDS             complement(7429..9900)
                     /locus_tag="ACFM8S_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFC6313596.1"
                     /translation="MKKTCFHCGLDVPEHLHLTVRYENEDRETCCAGCQAVAQSIIDA
                     GLGSYYKQRTADAQKTELPPQEILDQIRLYDLPEVQSDFVETHGGTREAVLMLGGITC
                     AACVWLIEQQLLRTDGIVRIDLNYSTHRCRVVWDDGKIRLSDILLKIRQIGYTAAPYD
                     AQKIEAANQKERKQYIVRLAVAGLGMMQTMMFALPTYLYGGDIEPDFLQILHWGGFLM
                     VLPVVFYCAVPFYQGALRDLKNRRVGMDTPITVAIIMTFIAGVYSLATNAGQGMYFES
                     IAMLLFFLLGGRFMEHIARRKAGDAAERLVKLIPAFCHHMPDYPDTQETCEAAVVKLK
                     AGDIVLVKPGETIPVDGTVLEGASTVDESMLTGESLPVAKMPSEKVTAGTLNTQSPLI
                     IRTDRTGGGTRLSHIVRLLDRALAQKPRTAELAEQYASSFIFGELLLAVPVFIGWTLY
                     ADAHTALWITVALLVITCPCALSLATPTALAASTGTLAREGILIGGKQAIETLAQTTD
                     IIFDKTGTLTQGNPAVRRIELLGSMTEAQVLAVAQSLEQQSEHPLARAILNCRISGGS
                     VPDIAIKQRLNRIGEGVGAQLTVNGETQVWALGRASYVAEISGKEPQTEGGGSAVYLG
                     SQSGFQAVFYLTDPLKDSAAEAVRQLAGKNLTLHILSGDRETAVAETARALGVAHYRA
                     QAMPEDKLEYVKALQKEGKKVLMIGDGINDAPVLAQADVSAAAAGGTDIARDGADIVL
                     LNEDLRTVAHLLDQARRTRHIIRQNLIWAGAYNIIAVPLAVLGYVQPWIAALGMSFSS
                     LAVLGNALRLHKRGKMQSEKMPSEQ"
     gene            complement(9897..11075)
                     /gene="hflX"
                     /locus_tag="ACFM8S_000475"
     CDS             complement(9897..11075)
                     /gene="hflX"
                     /locus_tag="ACFM8S_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFC6313597.1"
                     /translation="MTGRTGGNGSTQAQPERVMLVGVMLDKDGTGSSAARLNGFQTAL
                     AEAVELVKAAGGNPVIVETAKRDRPHTALFVGTGKAAELSEAVAADGIDLVVFNHELT
                     PTQERNLEKILQCRVLDRVGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHL
                     QSQRGGIGMKGPGETKLETDRRLIAHRINALKKQLANLKKQRALRRKSRESGTIKTFA
                     LVGYTNVGKSSLFNRLTKSGIYAKDQLFATLDTTARRLYISPECSIILTDTVGFVSDL
                     PHKLISAFSATLEETAQADVLLHVVDAAASNSGQQIEDVENVLQEIHAGDIPCIKVYN
                     KIDLLPSEEQNTGIWRDAAGKIAAVRISVAENTGIDALREAIAEYCATAPNTDETEMP
                     "
     gene            complement(11080..12360)
                     /locus_tag="ACFM8S_000476"
     CDS             complement(11080..12360)
                     /locus_tag="ACFM8S_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFC6313598.1"
                     /translation="MKSLFIRLLLLGSAAGVFYHTQNQSLPAGELVYPSAPQIRDGGD
                     ALHYLNRIRAQIGLHKLAHAPVLENSARRHARYLTLNPEDGHGEHHPDNPHYTAQKLT
                     ERTRLAGYLYNGVHENISTEEEAAESSDSDIRTQQRQVDGLMSAIYHRLSLLDRHTDE
                     AGAAFVRENGKTVLVFNQGNGRFERHCAQGRNQPEAGRKYYRNACHNGAVVYTDEAMP
                     AQELLYTAYPVGSGALPYFHGERPDPVPEYEITGNPASIDFSEAAGKITMKSFKLYQG
                     KNEIRPVRVLTAGNDPNGRLTAYQFALFPLKPLEYGTLYTAVFDYVRNGRRAQAKWQF
                     RTRKPDYPYFEVNGGETLAVRKGEKYFIHWRGRWCLEACTRYTYRQRPGSRLSIGRHE
                     AGGIVFSVGGMAGSSITLAPEDSPERGVTLYLQD"
     gene            12362..12490
                     /locus_tag="ACFM8S_000477"
     CDS             12362..12490
                     /locus_tag="ACFM8S_000477"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313599.1"
                     /translation="MPSSRRGNGVFYQNGKLANAVSDCRLPNRQTFPRADAEPDVV"
     gene            complement(12513..13238)
                     /locus_tag="ACFM8S_000478"
     CDS             complement(12513..13238)
                     /locus_tag="ACFM8S_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFC6313600.1"
                     /translation="METQNKPTVTDIDRPILVPPGGHKKVLLHSCCAPCSGEVMEAML
                     ASGIDYTIYFYNPNIHPHKEYMLRKEENVRFAEKFGIPFIDKDDDYENDRKEWFAKAK
                     GMEFEPERGIRCTMCFDMRFEKAAQYAHEHGFPVFTSSLGISRWKNMAQINDCGRRAA
                     APYDDVVYWDFNWRKGGGSARMIEISKRENFYQQEYCGCAYSLRDSNAHRKSQGRIPI
                     KLGVLYYGDESTQYEPAPIRVDK"
     gene            complement(13311..13988)
                     /gene="radC"
                     /locus_tag="ACFM8S_000479"
     CDS             complement(13311..13988)
                     /gene="radC"
                     /locus_tag="ACFM8S_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFC6313601.1"
                     /translation="MSIKQWPEGERPREKLLERGAAALSDAELLAILLRVGTRGMSAV
                     DLARYLLQEFGSLGRLMSAEVGKLSAYKGMGTASFTQFAVVREIGRRILAEELQESIV
                     LSDPDTVADYLRFHLGQEKVEVSVALLLNRQNQLIAVRELSRGTVAENTIYIREIVKL
                     ALDEYADSLIIAHNHPGGSPEPSQEDIMFTRRLAQAMSLVDVSLLDHFIVTAQTVRSF
                     RQLGLMP"
     gene            complement(14111..15460)
                     /gene="gshA"
                     /locus_tag="ACFM8S_000480"
     CDS             complement(14111..15460)
                     /gene="gshA"
                     /locus_tag="ACFM8S_000480"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFC6313602.1"
                     /translation="MKLPVMSPEHSAQLQAFEAKILSNHAKIEAWFRTQWNAHRPPFY
                     GSVDIRNAGYKISSIDMNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETAKSVLIIPE
                     NHTRNTFYLQNVYALGEILCSAGYEVRLGSLNPEVTEPTEFETALGDKILLEPLLRTR
                     DRVHLADGFSPCVVLLNNDLSAGIPDILKGISQTVLPPLHGGWTTRRKTNHFGAYNQV
                     TAEFAKLIGIDEWQINPYFEKIGGLDFQGREGEDALAEAVERVLAKIQAKYDESGITD
                     KPFVIVKADAGTYGMGVMSVKSADEVRGLNRKNRNKMAKVKEGLEVSEVIVQEGIYTY
                     ETLNGAVCEPVVYMMDRFVIGGFFRVHEGRGADENLNAGGMVFVPLSNSIPTGNGDNS
                     QEAPEACKRVFEQWDSLGMPRSEKDCDVDNEHNRLYVYGVMARLSLLAASIELEETA"
     gene            15720..17129
                     /gene="leuC"
                     /locus_tag="ACFM8S_000481"
     CDS             15720..17129
                     /gene="leuC"
                     /locus_tag="ACFM8S_000481"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFC6313603.1"
                     /translation="MTAQTLYDKLWNSHVVREEEDGTVLLYIDRHLVHEVTSPQAFEG
                     LKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPISKLQVDTLDKNIKEFGALAYFPF
                     MDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
                     TAKKSKSMLIAVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAIEFGGEAIRSLSMEGR
                     MTLCNMAIEAGARSGMVAVDQTTIDYVKDKPFAPEGEAWDKAVEYWRTLVSDEGAVFD
                     KEYRFNAEDIEPQVTWGTSPEMVLDISSKVPNPAEETDPIKRSGMERALEYMGLEAGT
                     PLNEIPVDIVFIGSCTNSRIEDLREAAAIAKDRKKAANVQRVLIVPGSGLVKEQAEKE
                     GLDKIFIEAGFEWREPGCSMCLAMNADRLTPGQRCASTSNRNFEGRQGNGGRTHLVSP
                     AMAAAAAVTGRFTDIRMMA"
     gene            17226..17480
                     /locus_tag="ACFM8S_000482"
     CDS             17226..17480
                     /locus_tag="ACFM8S_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313604.1"
                     /translation="MNKLFITALSALALSACAGTWEGAKQDTARNLDKTQAAAERAAE
                     QTGNAVEKGWDKTKEAVKKGGNAVGRGISHLGGKIENATE"
     gene            17542..18183
                     /gene="leuD"
                     /locus_tag="ACFM8S_000483"
     CDS             17542..18183
                     /gene="leuD"
                     /locus_tag="ACFM8S_000483"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFC6313605.1"
                     /translation="MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDNSKRPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDY
                     GFRAVIAPSFADIFFNNCYKNGLLPIVLTEEQVDRLFKEVEANEGYQLSIDLAEQTLA
                     TPGGETFTFDITEHRKHCLLNGLDEIGLTLQHADEIHAFEEKRRQSQPWLFNG"
     gene            18357..19628
                     /locus_tag="ACFM8S_000484"
     CDS             18357..19628
                     /locus_tag="ACFM8S_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC6313606.1"
                     /translation="MQQIKFIDLFSGMSGIRKGFEQACRKQSVACECVFTSEIKPAAL
                     EVLKQNYPDEVPYGDITKIETGDIPDFDILLAGFPCQAFSFAGKRLGFEDTRGTLFFD
                     VARILKAKKPKGFILENVEGLVTHDRKDPTQKIGRTLTVILETLETLGYYVSWKVLNA
                     KDFGIPQNRKRIYLTGSLKSKPDLSFETTLSPKLKNILESGLPTESSPFIKKLLKKFP
                     PSELYGKSVKDKRGGKNNIHSWDIELKGTVTEEEKQLLNMLLKERRKKKWASEIGIDW
                     MDGMPLTKAQISTFYKHPNLQNILDSLTDKGYLVLEHPKQKIGGRRIKDESLPKGYNI
                     VSGKKSFEINKILDPNDVAPTLVAMDMEHLFVVDNGGLRTLTGKEGLRLFGYPDDYSF
                     DIPKKDKYDLLGNTVAVPVIKAVSERLLHTL"
     gene            19644..20375
                     /locus_tag="ACFM8S_000485"
     CDS             19644..20375
                     /locus_tag="ACFM8S_000485"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HFC6313607.1"
                     /translation="MVKLTAQQIFDKLLDEEKILSANGQIRFFLGDVDIIVKQKDVVG
                     NIIQEWLGGWLRKREIEFDVSTNTQMPPDFFLNKKDRSRELLEVKAFNRNAGPGFDIA
                     DFKMYSDEIIHKPYMLDVDYLIFGYDMDDNGNVTIKDLWLKKVWQITRSMDGWAINLQ
                     VKKGVVHKIRPGVWYSINKKNMPMFECLEDFVSAIEETVYQNPATRHNASLWKKKFEE
                     AYKKHYNRSISIPRWHEIAHKYKKK"
     gene            20409..21479
                     /gene="leuB"
                     /locus_tag="ACFM8S_000486"
     CDS             20409..21479
                     /gene="leuB"
                     /locus_tag="ACFM8S_000486"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFC6313608.1"
                     /translation="MTKHIAILRGDGIGPEIVAETVRVLDKLIAQGLDAGYEYAPLGG
                     EAYDEYGHPYPEFTQNLCRKADAVLLGAVGSPQYDNLDRPLRPERGLLAIRKDLNLFA
                     NLRPAVLYPELANASTLKPEIVAGLDILIVRELTGDIYFGEPRGIRVLENGEREGYNT
                     MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREIFEEIGKEYPDVELS
                     HMYVDNAAMQLVRAPKQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLY
                     EPSHGSAPDIAGQNKANPLATILSLAMLLRYSLNDEARAQQVENAVQKVLQQGLRTSD
                     IYEEGTKLVSCSEMGDAVLAAL"
     gene            21642..22205
                     /locus_tag="ACFM8S_000487"
     CDS             21642..22205
                     /locus_tag="ACFM8S_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFC6313609.1"
                     /translation="MKKIIIAALAAAAIGTTSAATYKVDEYHANARFAIDHFNTSTNV
                     GGFYGLTGSVEFDQAKRDGKIDITIPVANLQSGSQHFTDHLKSADIFDAAQYPNIRFV
                     STKFNFNGKKLVSVDGNLTMHGKTAPVKLKAEKFNCYQSPMLKTEVCGGDFSTTIDRT
                     KWGMDYLVNVGMTKSVRIDIQIEAAKQ"
BASE COUNT         5375 a         5887 c         5806 g         5266 t
ORIGIN      
        1 ggcatttcca aagggcattt taagccgtat ttgagagaat gcggatggcg ttttcagtgt
       61 tttcagcgtg ataaaattca aatttctatt ttaaaacaat cagcaaagca ggatttattt
      121 cagttgtccg agacaacggg cggctgcacc gcctgcactc cccatacttt ttcaatgcac
      181 tcgagcagga gtgagacgtg gggattatcc tcgtattctt ccgcataaat gaaattgagc
      241 ggcatactgt ggcggcacga ttcgataata gccacgcccg cgccttcttt tgccgccgct
      301 tcggctttgt ttcccggcac cattgccaca cctataccgc ctgcaaccaa atcgataatg
      361 gtttgagggt agtcgcacaa gatctgtttt ttgggtgaga gccggttgct gcgccaaaac
      421 tggtgcaggt gcttcctact tccggacacg cccgacattt ctatccatac gcattcttgc
      481 aggctctgcg gaagggagcg ggtcagatgg ggatattggc tttgcgggca aatcagcgcg
      541 taggtcaggt tttgcaggaa tacgctgcgg atgccgcgtt gggcggcgtt gccgagtata
      601 aagccgccgt gcagggtttt gtgttggatg cgcgagagga tttcgccgct cattccgtat
      661 tggatgtgca ggcgcgtttt gggggctgtt tgaccgatat tgtccgtcag cgcgacgagc
      721 tttgccgaat cgatggggtg gataatgccc aaactggtct cttccgaata atcgcctgcc
      781 agcgttttgg caaaatactc caacttgtgt ttgtattgca gcagggattc cgcttcgggc
      841 aacagtattt cgcccgcccg cgtcaatacc atgcctttcc ccgtgcgcct gaacagcggc
      901 gtgccgacat attcttcaag ggctttaatt tgggcggaaa cggcaggctg ggaaaggaaa
      961 agtcgtttgg cggcttgggt aaggttgccc tcgtgcgcga cggcgacaaa tgattttaat
     1021 tgtacggcat ccatatatcc ctccttgtgc ggatgttttc tatatttgtg caatcgaaat
     1081 cttttaggcg gattgttgct gaaaattaac tttttaatca agtggtttgt aaattgtatc
     1141 agttttccgg atgatggtta ttaaaaaaaa agattggttt tgttgcctgt ttgggcttta
     1201 aatggggtta cggcttccga acgcagcccg tatcaaaaag aaaagtcatg cgcccctttt
     1261 acgaggcgcg atatataagg aggaaggtta tggaaaaaca taatgggact tatcgggatt
     1321 tgcaccgtcc agcttcggaa tttgcgacgc gggacgaata tttggaacat gaattgcaga
     1381 ttatgcaacc aaaacgctgg cggcccaacc tgccctttcg cgattaccgc ttcgagtggg
     1441 aggatttgat tcctgcgatg gcgggaacga ttggaaaagt ggtgatggtg ggggcggtgg
     1501 cggcggcgtt tgccgcacct ttggggctgc ctgacagctt tgtactggaa aatgtgcgct
     1561 atgagctttt aatcgccgcc gcgtttatct tattggtatc gggctttttt ctgcccggcg
     1621 ccaacctgcc cggtacgcac gggccgctga ttccgatgat tcccatcgtt gtgtcggcag
     1681 gcgggcatcc tttggcgttc ggcatttcga ttgcggtttt aggtctgctg atggctttat
     1741 ttcgcggcgg cagtattatg gcgaagctga caagcaacgg cgtatgcggc ggattattac
     1801 tctatttggg ctttatcggc acgacggggc aggtaaaaaa attgttttcg tgggcaggcg
     1861 gttttaatat gccctacatc gcttttaccg tcattattgt aacgattgtg atgtacgctt
     1921 tgttggagca ttggaaaaaa cgctggttag ccgtgccttt gggatgcttg attgccggtg
     1981 tggtggcatt tgcattgggt gcgccgtttg agtttcacac cgcccccggc ctgcctccaa
     2041 tgagtcctgc ttattggtgg ggtgaaaaca gcggctggca tctggggttg ccgacggcag
     2101 aaagtttttt ggttgtcttt ccatttgcgg tattggctgt tgcaatgtgg tcgcccgatt
     2161 ttttaggaca tcaagtgttc caaaaaatca gctatccgga aaaaaccgat aaggtattga
     2221 tgaatataga cgacaccatg acaagttgtt ctgtccgtca agcagtgggt tctattttag
     2281 ggggtgcaaa ttttacctct tcttggggaa cttatatcgt accggcatcg attgccaaac
     2341 gccccattcc gggcggtgcg gttttaacgg cggttttatg tattatcgcc gggttatggg
     2401 gctatccgat ggacttggcg atttggcagc cggtattgag cgtagccttg gtcgtaggcg
     2461 tatacttacc gcttttggaa gcgggcatgg aaatgacgcg caaaggcaaa accacccaat
     2521 ccgccgccat cgtggtgttc tcttccgcct tggtcaatcc ggttttcggc tgggcgttga
     2581 cgatgctgtt ggataatttg ggcttaatcg gctgcaaaga gcgcagtgcg caattaggtt
     2641 tcgccggacg cgtgttgata cccgcagtag gtttcttgat cttgtgtgtg gcgatgggtg
     2701 cggtcgggat gctgcccggt atcccgccgt ttttggaaca cttcaaatct ttgggctagg
     2761 ctgaaatcgg aaatgccgtc tgaaccgctt tcagacggca tttttgcaaa caggcaaaat
     2821 gacggcggcg ggatttttta ttttcccgat tgaagtataa tgttgccggg cttcaaccgg
     2881 atattcaaaa cggtttgttc caacactcgg aacggcgcat aaaacgccgc ccttcgcgtt
     2941 atcccgaacg gggcggctaa tcagattcta tcgccataaa aggcggggtt tcaaccgaaa
     3001 aggaattgag atgaataaaa ccttgtctat tttgccggtg gcaatcttac tcggcggctg
     3061 cgccgccggg ggcggtaaca cattcggcag cttagacggc ggcacaggca tgggcggcag
     3121 catcgtcaaa atggcggtcg ggagccaatg ccgtgcggaa ttggacaaac gcagcgaatg
     3181 gcgtttgacc gcgctggcga tgagtgccga aaaacaggcg gagtgggaaa acaagatttg
     3241 cgcttgcgtc gcccaagaag cacctgaacg gatgatcggc aacgatgtga tgcagatgct
     3301 ggatccgtcc acgcgcaatc aggcacttgc cgccctgacc gccaaaacgg tttccgcctg
     3361 cttcaaacac ctgtaccgct aaccttcttc agacggcatg aggcagatgc cgtctgaagc
     3421 cgttttcctg caatttcgac agacgagaaa aatcatgaaa tacatcagca cgcgcggcga
     3481 aaccgcacac aaaccgttca gcgaagtttt attgatgggg cttgcacccg acggcggcct
     3541 gatgctgccg gaacattatc cgcaaatcgg gcgcgaaacc ttggacaaat ggcgcggttt
     3601 agcttatccc gaattggcgt ttgaaatcat gcgcctgttt gttacggata ttccggagga
     3661 cgatttgcgc gacattctga accgtactta cacggaagcg gcgttcggta ctaaggaaat
     3721 cacccccgtc cgcacgcttt cagacggcat caaaatccaa gccttgtcca acggcccgac
     3781 gctggcgttc aaagatatgg cgatgcagtt tttgggcaat gcgtttgaat atgttttaaa
     3841 caaagaaggc aaaaaactca atatcttggg cgcaaccagc ggcgatacgg gttcggctgc
     3901 ggaatatgcc ttgcgcggca aaaaaggtgt gaatgtattt atgctgtcgc ccgatggcaa
     3961 aatgagcgcg ttccaacgtg cgcagatgta cagcctgcaa gacgggaata tccacaatat
     4021 cgccgtgaag ggtatgtttg acgactgtca ggatattgtg aaggcggtgc aaaacgatgc
     4081 cgcgttcaag gaaaaatacc atatcggtac ggtcaattcg attaactggg gacgcatcgt
     4141 cgcgcaagtg gtttattact ttgccggcta tttcaaagcg acgcaaagca atgacgagca
     4201 agtcagcttt tgcgtgccga gcggcaactt cggcaacgtt tgcgcgggac atattgccaa
     4261 acagatgggc ttgcccatcc gccgcctgat tgtcgcgacc aatgaaaacg atgtgttgga
     4321 cgagtttttc aaaaccggcg cgtaccgccc gcgcaacagc gcgcatactt atgttacctc
     4381 cagcccatct atggacattt ccaaagcgtc caatttcgag cgtttcgtgt tcgacctgat
     4441 ggatcgcgat cctcaggaaa tcaatacgct gtggacggaa gtcgcggcgg gcaagggctt
     4501 tgacttgcag tttgccttag acaaagtcgg cggcaaatac ggctttacct ccagcaagtc
     4561 cacccacgcc gaccgcctcg ccaccatcaa acaggtttac gagcaagacc aagaactcat
     4621 cgacccgcac actgccgacg gcgtaaaagt cgcccgcgaa gtgcgcgaag aaggggaaac
     4681 ggtggtttgt ttggaaaccg cgttggcagc gaaattcgat gcgaccatac gcgaagccgt
     4741 cggcgatgcc gccattccgc gccctgccgc gctggaaggt ttggaaaacc tgccgcagcg
     4801 cgtgcaaacc gtgccgaaca gtgcggatgc ggtaaaaggc atcatcgaac aaacccttgc
     4861 ctgatgttcc gtacaggggc attccgtccg aacttaagat tgaaagaaaa tgaccttacc
     4921 tatgcaggaa acccgttttt ccatactgct agacgaattg gcggcaaaac aggaggcagc
     4981 catcgccccc cacctgcttg ccgacggaac caaggtatgg atacgcaaag caggcaggca
     5041 caatgcgcga tggcgttacg cgctgctcgg tatggttgcc cgatatttga aactgggggt
     5101 gttgaagccg gttcccagcc tcggcggcga gcctgccatt acaaccgaat caaaacgcct
     5161 gtacgaattg cgcgcggcgg ggatagccgt tcccgaattg ctcgccgtcc ggaaaaatgc
     5221 ccttatgttc ggcaatttgg aaggcatccc gctcgatacg caaatccgcc aagaagccga
     5281 ggctggaaag tcggacgctt ggcttgccgg tttggaagcc attgcacgcg tgcataaaaa
     5341 acggcagttc ctcagtcagg cgtttgcgcg aaatatgatg tcggacggaa aaaacatcag
     5401 ctttttggat tttgaagacg acccttccga agtcctgacc atagcgcaat gccaagcccg
     5461 cgattggctc tgctacatcc attcgaccgc gctgatattg aaaaacggcg gactgctgga
     5521 agcggctgcg gaaaaatggg gcggcgtatt gtcggatcag cctgccgaaa tacaaaagct
     5581 gattgccgcc accgtcaaac cgatcctccc gatacgcagg ctggaacacc cacgctgggg
     5641 acgggacgcg ctcaggctgg cagcctcgat ttccctgatt tcccttgccg atatgccgcc
     5701 gtaagccgcc gcccgccgcg cgatgccgtc tgaagccctt cagacggcat ataaccaacg
     5761 cgcatcaagc attgtcgcaa ccatccgttt cgtttagaat tttggcttta tcccgaatcg
     5821 ggcagacatt tccactctgc cgcacctccc gaacaagcac tatccatttc ttttactgac
     5881 aggaaaccca aatggcagca ttcaataccc aaaaagtatt gtccgtacac cactggacag
     5941 acgcatattt tacctttacc tgcacccgcg acgaatcgtt gcgctttgaa aacggacagt
     6001 tcgttatggt cggattgatg gtggacggca agccgctgat gcgcgcatac agcgtcgcct
     6061 ccgccaactg ggaagaacac ctcgaatttt tcagcattaa agtccaagac ggtccgctga
     6121 ccagccgcct gcaacacctc aaagtcggcg acgacgtgtt aatcagcaaa aaaccgaccg
     6181 gaaccctggt tgccggcgac ctgaatccgg gcaaacacct ttacttgttg agcaccggta
     6241 ccggcatcgc tcctttcttg agcatcacca aagaccccga aatttacgag caatttgaaa
     6301 aaatcatcct cgtacacggc gtgcgctaca aaaaagattt ggcgtactac gaccgcttta
     6361 ccaaagaatt gcccgaacac gaatacctcg gtgacttggt taaagaaaaa ctgatttact
     6421 acccgattgt ttcgcgcgaa gaattcgaac accacggtcg cctaaccgac ctgatggtga
     6481 gcggcaaact gtttgaagac atcggcctgc ccaaaatcaa cccgcaagac gaccgcgcca
     6541 tgctgtgcgg cagccccgcg atgctgaaag atacctgcaa agttttggac gatttcggac
     6601 tgaccgtctc tccgaaaaca ggcgtacgcg gcgattacct gattgagcgt gcgtttgtag
     6661 atcaataagc tgtttgaaaa tcccgtctga acggcaccag caatcaaaaa ccccgaatcc
     6721 tgccaatcag gattcggggt ttttatagtg aattaaattt aaatcaggac aaggcggcga
     6781 gccgcagaca gtacaaatag tacggcaagg cgagccaaca ctgtaccggt ttaaatttaa
     6841 ttcactatat agtgggtaaa aattagaaat gcacatttcc gtcattcacg cgcaggcggg
     6901 aatccagacc tatccgttaa ttagcatttg aaaataaaag aaaaaccaag catatggatt
     6961 cccccctacg cgagactgac gacggtgttc tgcagtagcc aaagcagaaa accatcctta
     7021 ttgtcatttc atcatgatat aacaataagg atgttaaaaa ttagttatca ttatatgcat
     7081 gatatataag aaaaattgtc ccaataagaa atgttatgca gaaaataaaa tgaaaaggat
     7141 ggttttcatt gaaaatggtt gttctgccac cgtcatacac agaaccggta tttggaaaaa
     7201 aaataatcca tattgacatc attattccat ttatcgcata aatccacttt ttaatttttt
     7261 tcctcattaa tacaatctct tatttaataa aatcaaatac ctagcaattc gatctcaaat
     7321 tttgaacgcc aacgtgtttg agcggtaaca atacatactt taagtttgca attcttcatc
     7381 gtcttatttt tgttttcatc aactatacgt ctaaagcaac gccgtctgtc attgttcgga
     7441 cggcattttt tcagactgca ttttcccccg tttgtgaagg cgcagggcgt tgcccaaaac
     7501 cgccagcgaa ctgaagctca tacccagtgc ggctatccac ggttggacat agcccaaaac
     7561 ggcaagcggt acggcaatga tattgtacgc gcccgcccat atcaggtttt gccggataat
     7621 atggcgggtg cgccgcgcct gatcgagcag gtgggcgacg gtacgcaaat cttcgttcaa
     7681 taacacaatg tccgcgccgt ccctcgcaat atccgtcccg cccgctgcgg cggcggatac
     7741 gtctgcctgc gccaaaacgg gcgcgtcgtt gatgccgtcg cctatcatca gcactttttt
     7801 cccttctttt tgcaaggctt tgacgtattc cagtttgtcc tcgggcatgg cttgggcgcg
     7861 gtagtgcgcg acacccaggg cgcgtgcggt ttcggcaacg gcggtttcgc ggtcgccgct
     7921 gaggatgtgc agggtcaggt ttttgcctgc caactgccgc accgcctccg ccgcgctgtc
     7981 tttcaagggg tcggtcaggt agaacacggc ttggaaaccg ctttgactgc cgaggtaaac
     8041 cgcgctgccg ccgccttctg tttgcggttc tttacctgaa atttcggcga cataggatgc
     8101 cctgcccaat gcccaaacct gtgtttcgcc gttgacggtc agttgcgcgc ccacgccttc
     8161 gccgatgcgg ttgaggcgtt gtttaatagc aatgtcgggg acgctgccgc ctgaaatgcg
     8221 gcagttgagg atggcgcgcg cgagcgggtg ttcggactgc tgttccaaac tttgcgccac
     8281 tgccagcact tgggcttcgg tcatgctgcc gagcagttca atccggcgga cggcgggatt
     8341 gccttgggtc agcgtgcctg ttttgtcgaa aatgatgtcg gtggtttggg cgagggtttc
     8401 gattgcctgc tttccgccga ttaaaatacc ttcgcgcgcc agcgtaccgg tagaagctgc
     8461 cagcgcggtc ggcgtggcaa gcgataaggc gcaggggcag gtaatgacca gcagggcgac
     8521 ggtaatccac aatgcggtgt gcgcgtcggc gtacagcgtc cagccgatga agactgggac
     8581 ggcaagcagg agttcgccga atatgaaaga cgaggcatat tgttccgcca actcggcggt
     8641 gcgcggtttt tgcgccaagg cgcggtcgag caggcggacg atgtgcgaca gtcgcgtgcc
     8701 gccgccggtg cggtcggtgc gtataatcag ggggctttgc gtgttgagtg tacctgcggt
     8761 tactttttca gacggcattt tggcgacggg caggctctcg ccggtcagca tagattcgtc
     8821 aaccgtgctt gcgccttcca gcaccgtgcc gtcaacgggg atggtttcgc ccggtttgac
     8881 cagcacgata tcgcccgcct tcaatttgac gacagctgcc tcgcaggttt cctgcgtatc
     8941 ggggtaatcg ggcatatgat ggcaaaacgc aggaatcagc ttcaccagcc tttcggcggc
     9001 atcgcctgcc ttgcgtcggg caatgtgttc cataaagcgt ccgcccagca ggaaaaacag
     9061 cagcatggct atggattcga aatacatccc ctgccccgca ttggtcgcaa ggctgtaaac
     9121 gccggcgata aaggtcatga tgatggcgac ggtaatcggc gtatccatgc cgacgcggcg
     9181 gtttttcaag tcgcgcagcg cgccttgata aaacgggacg gcgcaataga atacgacggg
     9241 cagcaccatt aaaaagccgc cccaatggag gatttgcagg aaatcgggtt cgatgtcgcc
     9301 gccgtaaagg taggtcggca gcgcgaacat catcgtctgc atcatcccca gcccggcaac
     9361 ggcgaggcgg acgatgtatt gtttgcgttc tttttggttg gcggcttcga ttttttgcgc
     9421 gtcatagggt gcggcggtgt agcctatctg cctgattttc aacagaatgt cggaaaggcg
     9481 gattttgccg tcgtcccaga cgacgcggca gcggtgcgtg ctgtaattga ggtcgatgcg
     9541 gacgatgccg tctgtacgca aaagctgctg ttcgatcagc cagacgcagg cggcgcaggt
     9601 gatgccgccg agcattaaaa ccgcctcgcg cgtgccgccg tgggtttcca caaagtcgga
     9661 ctggacttcg ggcaggtcgt acaggcggat ttggtcgagg atttcttggg gcggcagctc
     9721 ggttttttgc gcgtcggcgg tgcgttgttt gtaataactg cccaagcccg cgtcaataat
     9781 gctttgtgcg accgcctgac agccggcgca gcaggtttcg cggtcttcgt tttcgtaacg
     9841 gacagtcagg tggaggtgtt cgggaacatc cagaccgcag tggaaacagg tttttttcat
     9901 ggcatttcgg tttcgtctgt gtttggtgcg gtggcacaat actcggcaat ggcttcgcgc
     9961 agtgcgtcta taccggtatt ttcagcaacg gaaatgcgga cggcggcaat ttttcccgca
    10021 gcgtcgcgcc atatgcccgt gttttgttct tcagacggca gcaggtcgat tttgttgtac
    10081 accttgatgc acggaatatc gccggcatgg atttcttgca gtacgttttc cacgtcttca
    10141 atctgctgcc cgctgttcga ggcggcggca tcgacgacgt gcagcagcac atcggcttgc
    10201 gcggtttctt ccagcgtggc ggaaaatgcc gaaatcagtt tgtgcggcaa atcactgacg
    10261 aatccgacgg tatcggtcag gataatgctg cattcgggac tgatgtacag ccgccgcgcc
    10321 gtggtgtcga gtgtggcgaa aagctggtct ttcgcatata tgcccgactt ggtcagccgg
    10381 ttgaacaggc tggatttgcc gacattggta tagccgacca gcgcaaacgt tttgattgtg
    10441 cccgattcgc gggacttgcg gcgcagggcg cgctgttttt tgaggttggc aagctgtttt
    10501 ttcaaggcgt tgattcgatg ggcgatcaat cggcggtcgg tttccagttt ggtttcgccc
    10561 ggccctttca tgccgatacc gccgcgctgg ctctgcagat ggccgtaacc gcgtatcaag
    10621 cgtcccgcca aatggctcaa ttgcgccaac tcgacttgca gcctgccttc ctgcgtgcgg
    10681 gcgcggcggg cgaaaatcgc caaaatcaac cctaccctgt ccaatacgcg gcattggagg
    10741 attttttcca aattgcgttc ctgcgtgggc gtaagttcgt ggttgaatac gaccaaatcg
    10801 atgccgtctg cggcaactgc ttccgacagc tccgccgcct tgcccgtgcc gacaaacagc
    10861 gcggtgtgcg gacggtcgcg tttggcggtc tccacgatga cgggattgcc gcccgccgcc
    10921 ttgaccagct cgacggcttc cgccaatgcc gtctgaaagc cgttcagacg ggcggcgctg
    10981 ctgcccgtac cgtctttgtc caacattacg cccaccagca tgacgcgttc gggttgcgct
    11041 tgggtactgc cgttgccgcc tgttctgcct gtcatgtatt caatcctgta aataaagggt
    11101 aacgccgcgt tccgggctgt cttccggtgc aagcgtgatg ctgctgcccg ccattccgcc
    11161 aacgctgaag acgatgccgc ccgcctcgtg ccttcctatg gacaggcggc tgccgggtcg
    11221 ctgccggtag gtataacggg tacacgcttc caaacaccag cgtccgcgcc agtggatgaa
    11281 atatttttcg ccttttctaa ccgcaagtgt ctcgccgccg tttacctcaa aataagggta
    11341 atcgggtttt cgggttctaa actgccattt cgcctgcgcg cgccgtccgt tgcggacata
    11401 gtcgaatacc gccgtataaa gcgtaccgta ttccaaaggc ttgagcggga aaagcgcgaa
    11461 ttggtacgcg gtcagcctgc cgttggggtc gttgccggcg gttaaaaccc tgacggggcg
    11521 gatttcgttt ttaccctgat acagcttgaa acttttcatc gtaattttgc ctgccgcctc
    11581 ggaaaaatca atgctggcag gattgcccgt gatttcatat tccggcacgg ggtctggacg
    11641 ctcgccgtgg aaataaggca gtgcgccgct gccgacggga taggctgtat agagcagctc
    11701 ctgtgcgggc atggcttcgt cggtgtacac gaccgcaccg ttatggcagg cgttgcggta
    11761 atatttccgt cctgcttccg gctgatttct gccttgggcg caatgccgct caaacctgcc
    11821 gttgccctga ttgaatacga gaacggtttt accgttttcg cgcacaaatg ccgctcctgc
    11881 ctcatccgta tggcggtcaa gtagggaaag gcggtggtag attgcgctca ttaatccgtc
    11941 cacttggcgt tgctgcgtgc ggatgtcgct gtcggacgat tcggcggctt cctcttccgt
    12001 gctgatgttt tcatgcacgc cgttgtagag atacccggca aggcgtgtgc gttcggtcag
    12061 cttttgtgcg gtgtagtgcg gattgtcggg atggtgttcg ccgtgtccgt cttcgggatt
    12121 gagcgtgaga tagcgtgcgt gcctgcgggc ggaattttcc aaaaccggcg cgtgtgccag
    12181 cttgtgcaaa ccgatttggg cgcggatgcg gttgaggtag tgcagcgcat cgccgccgtc
    12241 cctgatttgc ggtgcggacg gatagacaag ttcgcccgcg ggcagggatt ggttttgggt
    12301 atgatagaaa acgcctgccg ccgaacccaa caggagcagc cgaataaaaa gggatttcat
    12361 aatgccgtcc tcccgacgcg gcaacggggt gttttatcaa aacggcaaac ttgccaatgc
    12421 ggtttccgat tgcagattgc caaaccggca aacctttccc cgtgccgatg ccgaacctga
    12481 tgtcgtctga accttcagac ggcatcggat gtttatttgt ccacccggat gggtgcaggt
    12541 tcgtattgtg tcgattcgtc gccgtaatac agcacgccga gtttgatggg gattctgccc
    12601 tgtgatttgc ggtgggcatt ggaatccctc agggaatagg cacaaccgca atattcctgc
    12661 tggtagaagt tttcacgttt gctgatttca atcatgcgcg cgctgccgcc gcctttgcgc
    12721 cagttgaaat cccaatacac cacatcatcg taaggcgcgg cggcacggcg gccgcagtcg
    12781 ttgatttgcg ccatattttt ccagcgtgaa atgcccagcg aactggtaaa gacggggaac
    12841 ccgtgttcat gcgcgtattg cgccgccttt tcaaaacgca tatcgaaaca catggtgcag
    12901 cggatgccgc gctcaggttc gaattccata cctttggctt tggcaaacca ttctttgcgg
    12961 tcgttttcgt agtcgtcgtc tttatcgatg aaaggaatgc cgaacttttc cgcaaagcgc
    13021 acgttttcct cttttcggag catatactct ttgtgcggat ggatattggg attgtaaaaa
    13081 taaatggtgt agtcgatgcc ggaggcaagc atcgcctcca tcacttcgcc gctgcacggg
    13141 gcgcagcagg aatgcagcaa gactttttta tgtccgccgg gcgggacgag tatggggcgg
    13201 tcaatgtcgg taacggtagg tttgttttgt gtttccatta acttaacaat atctgtcgaa
    13261 tacggttgaa gcggctattt tatcagagcc gccgcatgta aaacaggggg tcagggcatc
    13321 agcccgagct gcctgaacga acggacggtt tgcgccgtta cgataaaatg gtcgagcagc
    13381 gacacatcga ccagcgacat tgcctgtgcc agccgccttg tgaacatgat gtcttcctgc
    13441 gaaggttcgg gcgagccgcc cggatggttg tgcgcaataa tcaggctgtc ggcatattcg
    13501 tccaatgcca gtttgacgat ttcgcggatg taaatcgtgt tttccgcaac cgtaccgcgc
    13561 gacagctctc tgaccgcaat cagttggttt tggcggttca gcagcagcgc gacgctgact
    13621 tcgacttttt cctgccccaa atgaaagcgt aaataatcgg ccaccgtatc cggatcggac
    13681 aggacgatgc tctcctgcaa ttcttccgcc aatatccgcc gcccgatttc cctgaccacg
    13741 gcaaactgtg tgaaacttgc cgtccccatc cctttgtatg ccgacagttt tccgacctcc
    13801 gcgctcatca gcctccccaa actgccgaac tcctgcagca aataacgcgc caaatcgacc
    13861 gcactcattc cgcgcgtgcc gacgcgcagc aggattgcca aaagttcggc atcactcaac
    13921 gccgccgccc cgcgttccaa cagcttttcc ctgggtcttt cgccttccgg ccattgcttg
    13981 atgctcatgt tttccctttt ttaaatagtc tgagatgccg tctgaaacag atgttcccac
    14041 acgccaaata tccgaaatgc cgtctgaacc ggatttccgc ttcagacggc atctgtattt
    14101 caaaacagtc ttacgccgtt tcttccaact cgatggacgc cgccagaagc gacaggcgtg
    14161 ccattacgcc gtaaacgtag aggcggttgt gttcgttgtc cacgtcgcag tctttttcag
    14221 agcgcggcat acccagcgag tcccattgtt cgaatacgcg cttgcaggct tcgggcgctt
    14281 cttgggagtt gtcgccgttg ccggtgggga tgctgttgga cagcggaaca aacaccatac
    14341 cgccggcgtt gaggttttcg tccgcaccgc gcccttcgtg tacgcggaaa aagccgccga
    14401 tgacgaagcg gtccatcata tacacgacgg gttcgcacac cgcgccgttt aaggtttcat
    14461 aagtataaat cccttcttgg acaatcactt cgctgacttc caagccttct ttgactttcg
    14521 ccattttatt gcggttttta cggttcaatc cgcgcacttc gtcggcggat ttgacgctca
    14581 tcacgcccat gccgtaagtg ccggcatcgg ctttgacgat gacgaaaggt ttgtcggtaa
    14641 tgcccgattc gtcgtatttg gcttgaattt tcgccagcac acgttctacc gcttccgcca
    14701 acgcgtcttc gccttcacgc ccttggaagt ccaaaccgcc gattttttca aaataagggt
    14761 taatttgcca ttcgtcgatg ccgattaact tggcaaattc ggcggtaact tggttgtacg
    14821 cgccgaaatg atttgttttg cggcgcgtcg tccaaccgcc gtgcaacggc ggcaaaacgg
    14881 tttggctgat gcctttgagg atgtcgggaa tgccggcgga caaatcgttg ttcaacaaaa
    14941 ccacgcaagg cgaaaagccg tctgcaagat ggacgcggtc gcgggtacgc aataaaggtt
    15001 ccaacaggat tttgtcgccc aatgcggttt caaattcggt cggttcggtt acttccggat
    15061 tcaggctgcc caagcgcact tcataccctg ccgaacacaa aatctcgccg agggcgtaaa
    15121 cgttttgcag gtaaaacgta ttgcgcgtgt ggttttcggg aataatcaat acggatttcg
    15181 ccgtttcgca ggcgcgttgc accgcatctt gcgcggcaac cgccgccagc gggataaagt
    15241 tgggattcag attattgaag ccgccgggga acaaattcat atcgatagac gaaattttgt
    15301 aaccggcatt gcgtatatcg accgaaccgt aaaacggcgg gcggtgcgcg ttccactgcg
    15361 tgcggaacca cgcctcgatt ttggcgtgat tggacaggat ttttgcctca aacgcctgaa
    15421 gttgcgccga atgttcgggc gacatcaccg gtaatttcat ctttcgtctg cctctgtgtg
    15481 ttttttgaca agatgcagat gataatgcct ttgaaagaat gacgacaagt attctttcgt
    15541 atcgaatcat gctttagata taactgttta ctgaaatgga caatatgtct aattttttcg
    15601 ttaaaattgt ttgcaaaata acaccggctc aaaaattaga caaaatttgt tgcgcggtat
    15661 aaagaatacg tctaaaatcc gcccaatccg cattaccatt tatccaaaag aacaacatca
    15721 tgacagcaca aaccctctac gacaaacttt ggaacagcca cgtcgtccgc gaagaagaag
    15781 acggcaccgt cctgctctac atcgaccgcc atttggtgca cgaagttacc agccctcagg
    15841 catttgaagg cttgaaaatg gcggggcgca agctgtggcg catcgacagc gtcgtctcca
    15901 ccgccgacca caacaccccg accggcgatt gggacaaagg catccaagac ccgatttcca
    15961 agctgcaagt cgatactttg gacaaaaaca ttaaagagtt tggcgcactc gcctattttc
    16021 cgtttatgga caaaggtcag ggcatcgtac acgttatggg ccccgaacaa ggcgcgaccc
    16081 tgcccggtat gaccgtcgtc tgcggcgact cgcacacttc cacccacggc gcattcggcg
    16141 cactggcgca cggcatcggc acttccgaag tcgagcacac catggcgacc caatgtatta
    16201 ccgcgaaaaa atccaaatcc atgctgattg ccgttgacgg caaattaaaa gcgggcgtta
    16261 ccgccaaaga cgtggcgctc tacatcatcg ggcaaatcgg cacggcaggc ggtacaggct
    16321 acgccatcga gtttggcggc gaagccatcc gcagcctttc tatggaaggc cgcatgactt
    16381 tatgcaatat ggcgattgag gcaggcgcgc gctcaggcat ggttgccgtc gaccaaacca
    16441 ccatcgacta cgtaaaagat aaacccttcg cacccgaagg cgaagcgtgg gacaaagccg
    16501 tcgagtactg gcgtacgctg gtgtctgacg aaggtgcggt attcgacaaa gaataccgtt
    16561 tcaacgccga agacatcgaa ccgcaagtca cttggggtac ctcgcctgaa atggttttag
    16621 acatcagcag caaagtgccg aatcctgccg aagaaaccga tccgatcaaa cgcagcggta
    16681 tggaacgcgc ccttgaatac atgggcttgg aagccggtac gccattaaac gaaatccccg
    16741 tcgacatcgt attcatcggc tcttgcacca acagccgcat cgaagacttg cgcgaagccg
    16801 ccgccatcgc caaagaccgc aaaaaagccg ccaacgtaca gcgcgtgtta atcgtccccg
    16861 gctccggttt ggttaaagaa caagccgaaa aagaaggctt ggacaaaatt ttcatcgaag
    16921 ccggttttga atggcgcgaa ccgggctgct ctatgtgcct cgccatgaat gccgaccgcc
    16981 tgaccccggg tcaacgttgt gcctctactt ccaaccgcaa ttttgaaggc cgtcaaggca
    17041 acggcggacg tacccacctc gtcagccctg ccatggcagc cgcagccgca gtgaccggtc
    17101 gctttaccga catccgcatg atggcgtaag tattttcagg cggtctttaa gaatttgacg
    17161 cggccgtctg aaatatgtcc cgtaaggcag aaacaccttt aatattcaaa ccaaggagtt
    17221 taaccatgaa caaacttttc attaccgccc tgtccgccct cgccttgtcc gcctgcgccg
    17281 gcacttggga gggcgcgaaa caagacaccg cccgcaacct tgacaaaaca caggccgccg
    17341 ccgaacgcgc cgccgaacaa acaggcaacg ccgtcgaaaa aggctgggac aaaaccaaag
    17401 aagccgtcaa aaaaggcggc aatgccgtcg gacgcggcat ttcccatctc ggcggaaaaa
    17461 tcgaaaacgc caccgaataa ccgattcgcg atgccgtctg aaaccgcttc agacggcatc
    17521 gtcagcacaa gacgcaaacc catgaaagcc tttaccaaaa tcaccgccat cgtcgccccg
    17581 ctcgaccgca gcaacgtcga taccgacgcc atcatcccca aacaattttt aaaatccatc
    17641 aaacgcagcg gcttcggccc caatgccttt gacgaatggc gttacctcga ccacggcgaa
    17701 ccaggcatgg acaacagcaa acgcccgttg aaccctgatt tctcgctgaa ccagccgcgt
    17761 taccaaggcg cgcaaatcct gttgacacgt aaaaacttcg gttgcggctc ttcacgcgaa
    17821 cacgcccctt gggcattgga cgactacggc ttccgcgccg tcatcgcccc cagcttcgcc
    17881 gacatcttct tcaacaactg ctacaaaaac ggccttttac ctatcgtttt gacagaagaa
    17941 caggttgacc ggcttttcaa agaagttgaa gccaacgaag gctatcagct ctccatcgac
    18001 cttgccgaac aaaccctggc cacccctggc ggcgaaacat tcacattcga cattaccgaa
    18061 caccgcaaac actgcctctt aaacggcttg gacgaaatcg gcctgaccct gcaacacgcc
    18121 gacgaaattc acgcctttga agaaaaacgc cgccaaagcc agccttggct gtttaacggt
    18181 taaacctatg ccgtctgaag gccgttcaga cggcattcct gataacatat aattgctgcg
    18241 gctaatacaa acagaggggt gataaattgc tttcatcttt taagcgggct tagcgacact
    18301 ctaaattaat gcaataatgc aactctccat ttcaaataaa tttttagatt tttaatatgc
    18361 aacagattaa atttattgac ttattttccg ggatgagcgg tatcaggaaa ggatttgaac
    18421 aagcctgtcg gaaacagtcg gttgcttgcg aatgtgtttt tacctcagaa atcaaaccgg
    18481 cagctttgga agtattgaag caaaactacc ctgatgaagt gccgtatgga gatataacga
    18541 aaattgaaac gggagatatt cccgattttg acatcttgtt ggcaggcttc ccttgccagg
    18601 ctttttcttt cgccggaaaa agattgggct ttgaggatac gcggggaacg cttttctttg
    18661 atgtggcaag gattttaaag gcaaaaaaac caaaaggttt tattttggaa aatgtggaag
    18721 gattggtgac gcacgataga aaagatccga cacaaaaaat aggacgcacc ctgaccgtta
    18781 ttttggaaac cttggaaaca ttgggctact atgtttcttg gaaagtttta aatgcaaaag
    18841 atttcggcat tccccaaaac aggaagcgca tttatctgac aggcagtttg aaatccaaac
    18901 ccgacttgtc ttttgaaaca accctaagtc cgaaattaaa aaatattttg gaatcgggac
    18961 tgcctaccga aagcagtcct ttcatcaaaa aattgctaaa gaaatttccc ccgtccgaac
    19021 tgtacggaaa atcagtaaag gacaaacggg gagggaaaaa caatatccac agttgggata
    19081 ttgaattaaa aggcacagta accgaagaag agaagcaatt attaaatatg cttctaaaag
    19141 aacggaggaa gaaaaaatgg gcttcagaaa tcggcatcga ttggatggat gggatgcctt
    19201 tgacaaaagc gcaaatttca actttctata aacaccccaa tcttcaaaat attttggaca
    19261 gcctgacaga taaaggctat ttggttttgg agcatccgaa acaaaaaatt ggcgggcgaa
    19321 gaatcaaaga tgaatccctg cccaaaggct acaatattgt ttcaggaaaa aaatcatttg
    19381 aaatcaataa aatattagac ccaaatgatg ttgcgccaac cttagttgca atggatatgg
    19441 agcacctttt cgtcgttgac aacggcggtt tgcgcacact taccggaaaa gaaggtttac
    19501 gcttattcgg ctatccggac gattattcgt ttgatattcc taaaaaagac aaatacgatt
    19561 tattgggcaa taccgttgcc gttcctgtga ttaaggcggt atctgaaagg cttctgcata
    19621 ctttataaac cgatagggat aaaatggtaa aacttactgc acaacaaata tttgataagt
    19681 tgctggatga ggaaaaaatc ttatcggcta atggtcaaat tagatttttc ttgggcgatg
    19741 tggatattat cgtcaaacaa aaagatgttg tcggcaacat tattcaggaa tggctcggcg
    19801 gatggttgag aaaaagggaa attgaatttg atgtttcaac caatacccaa atgcctcccg
    19861 atttcttttt aaataaaaaa gaccgcagca gagaattgtt ggaagtaaag gcgttcaacc
    19921 gaaatgccgg cccgggtttt gatattgcag attttaaaat gtattctgat gaaatcattc
    19981 ataagcccta tatgctggat gtagactatt taatattcgg ttacgatatg gacgacaacg
    20041 gcaatgtaac catcaaggat ttatggctta aaaaagtatg gcaaattacg agaagcatgg
    20101 atgggtgggc aattaatctt caagtcaaaa aaggcgtggt gcataaaatc cgcccgggtg
    20161 tttggtacag cataaataaa aagaatatgc ctatgtttga atgcttggaa gattttgttt
    20221 ccgcaattga agaaaccgtt tatcaaaacc cggcaacacg gcataacgca tccttatgga
    20281 aaaagaaatt tgaggaagcc tataaaaaac actacaaccg atcgatttcc atacctcgtt
    20341 ggcacgaaat tgcacacaaa tacaaaaaga aataaccctt caaccatcaa cccataagga
    20401 aacttgaaat gaccaaacat atcgccatcc tccgaggtga cggcatcggt cccgaaatcg
    20461 tcgccgaaac cgtccgcgta ctcgacaaac tcatcgccca aggtttggat gccggctacg
    20521 aatacgcccc attgggcggc gaagcctatg acgaatacgg tcatccttat cccgaattta
    20581 cgcaaaacct ctgccgcaaa gccgatgccg tcctgcttgg tgcagtcggt tctcctcaat
    20641 acgacaacct cgaccgtccg ttgcgccctg agcgcggcct gttggcaatc cgtaaggatt
    20701 tgaacctgtt tgccaatttg cgtcctgctg ttttatatcc cgaattagcc aacgcctcca
    20761 cgctgaagcc tgaaatcgtt gccggtttgg atattttgat tgtgcgcgaa ctcaccggcg
    20821 acatctattt cggcgaaccg cgcggcatcc gcgttctgga aaacggcgaa cgcgaaggct
    20881 acaacaccat gaaatacagc gaaagcgaaa tccgccgtat tgcccatgtt gccttccaat
    20941 ccgcccaaaa acgcagcaaa aaagtctgtt ccgtaggcaa agccaacgtt ttggaaacca
    21001 ccgaattgtg gcgcgaaatc tttgaagaaa tcggcaaaga gtaccctgat gtcgagcttt
    21061 cccatatgta cgtcgacaac gccgccatgc agctcgtgcg tgcgcccaaa caattcgacg
    21121 tcattgccac cggcaacatc ttcggcgaca ttctctccga cgaagcctcc atgctgaccg
    21181 gctccatcgg tatgctgcct tccgcctcgc tggacgaaaa cggcaaaggc ctgtacgaac
    21241 cgtctcacgg ctccgccccc gacatcgccg gacaaaacaa agccaacccg ctggccacca
    21301 tcctctcgct cgcaatgttg ctgcgttaca gcctgaacga cgaagcgcgc gcgcaacaag
    21361 ttgaaaacgc cgtccaaaaa gtgctgcaac aaggcttgcg taccagcgac atttacgaag
    21421 aaggcacaaa actcgtttcc tgctccgaaa tgggcgatgc ggtactcgcc gccttgtaaa
    21481 aaaacaaagc gcggatcaca ccgcgctttt tcatccgccc ttttccgccc gccccgttcg
    21541 tcaaccgcct gcaaacccac cgtttaccgg caggaaatgc cataaatccc tgcctatgtt
    21601 aggataaacg cctttaccgg cccacacaaa ggattatcat catgaaaaaa atcatcatcg
    21661 ccgcgctcgc agccgccgcc atcggcacta cctccgccgc cacctacaaa gtggacgaat
    21721 atcacgccaa cgcccgtttc gccatcgacc atttcaatac cagcaccaac gtcggcggtt
    21781 tttacggtct gaccggttcc gttgagttcg accaagcaaa acgcgacggt aaaatcgaca
    21841 tcaccattcc cgttgccaac ctgcaaagcg gttcgcaaca ctttaccgac cacctgaaat
    21901 ccgccgacat cttcgatgcc gcccaatatc ctaacatccg cttcgtttcc accaaattca
    21961 acttcaacgg caaaaaactg gtttccgttg acggcaacct gaccatgcac ggcaaaaccg
    22021 cccccgtcaa actcaaagcc gaaaaattca actgctacca aagcccgatg ttgaaaaccg
    22081 aagtttgcgg cggcgacttc agcaccacca tcgaccgcac caaatggggc atggactacc
    22141 tcgttaacgt tggtatgacc aaaagcgtcc gcatcgacat ccaaatcgag gcagccaaac
    22201 aataaatctt gatgcgaatc agcacaaatg ccgtcttcat caaaccgccc cgccaacggt
    22261 aagccgatat ggcggagcac aatccgccga aagatatttc agcggcaggc agggcttgcc
    22321 ggaaagtccg gaaa
//
LOCUS       DAXHSF010000016        21642 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.016, whole genome shotgun sequence.
ACCESSION   DAXHSF010000016 DAXHSF010000000
VERSION     DAXHSF010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21642
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.016"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(49..1245)
                     /gene="odhB"
                     /locus_tag="ACFM8S_000488"
     CDS             complement(49..1245)
                     /gene="odhB"
                     /locus_tag="ACFM8S_000488"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFC6313610.1"
                     /translation="MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKV
                     VLEVPSPQAGVLVEIVAQDGETVVADQVLARIDTAATAAAEAPAAATAAAEAPAAAPA
                     EAAPAAAPAAAQNNAAMPAAAKLAAESGVDVNALQGSGRDGRVLKEDVQNAAAKPAAA
                     AAPAGARPEERVPMSRLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKYKEKFE
                     KEHGVKLGFMSFFVKAAVAALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRGLVVPI
                     LRDADQMSIADIEQAIVDYAKKAKDGKIAIEDLTGGTFSITNGGTFGSMMSTPIINPP
                     QSAILGMHATKERAVVENGQVVVRPMMYLALSYDHRIIDGREAVLTLVAIKDALEDPA
                     RLLLDL"
     gene            complement(1345..4173)
                     /locus_tag="ACFM8S_000489"
     CDS             complement(1345..4173)
                     /locus_tag="ACFM8S_000489"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFC6313611.1"
                     /translation="MMDEKLNFSYLFGSNAPYIEELYEAFLENPDAVDEKWKQYFTDL
                     SKQPGTVAVDVAHTPIRESFVTLAKKKIASAVAGSADEAMLKKQVSVLRLISAYRIQG
                     VGAAQLDPLKRIPPRDIEALDPKFHGLSDADMALQFNMGEGDFANRGKLPLSQIISNL
                     KQTYCGHIALEYIYIPNTEERRWVRNYFESVLSTPHYNADQKRRILKEMTAAETLERY
                     LHTKYVGQKRFGVEGGESAIAGLNYLIQNAGKDGVEEVIIGMAHRGRLNVLVNILGKK
                     PGDLFAEFEGRAEIKLPSGDVKYHMGFSSDIATPHGPMHVSLAFNPSHLEIVNPVVEG
                     SARAKQKRLGENGRDKVLPVLIHGDSAFIGLGVNQATFNLSKTRGYTTGGTVHIVINN
                     QIGFTTSDIRDTRSTVHCTDIAKMVSAPVIHVNGDDPERVCFAIQAALDYRKKFHKDI
                     VIDVVCYRKWGHNEGDDPTLTQPMMYKKVSQHPGARALYTEQLIAEGVVTQAEADGYI
                     QAYRDALDKGEHVEQTTLSNFQRTQIDWSKYQGKDWREHIETGLPASDIERLTEKFTA
                     VPEGFALHPTARRVIEARKAMASGKQAIDWGMAETLAYASLVTKGHGVRISGEDSGRG
                     TFSHRHAVLHDQKREKWDDGTYVPLRHMGEGMGEFLVIDSILNEEAVMAFEYGFACSA
                     PDKLTIWEAQFGDFANGAQVTIDQFLSSGETKWGRLCGLTTILPHGYDGQGPEHSSAR
                     VERWLQLCSENNMQVIMPSEASQMFHLLQRQVLGSYRKPLVIFMSKRLLRFKGAMSPL
                     ENFTEGSTFRPVIGDTAERASNDSVKRVVLCAGQVYYDLETGRAERKLEDDVAIVRVE
                     QLYPFPYEEVKAELAKYPNAKSVVWAQEEPKNQGAFYQIRHRIEDVISEEQKLSYAGR
                     PSSASPAVGYSSKHIAQLKQLVEDALAL"
     gene            complement(4378..5661)
                     /gene="gltA"
                     /locus_tag="ACFM8S_000490"
     CDS             complement(4378..5661)
                     /gene="gltA"
                     /locus_tag="ACFM8S_000490"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFC6313612.1"
                     /translation="MSKSIKLNVPGQAGLELPVLEASIGHDVVDIRGLTKNTGLFSFD
                     PGFVSTASCESKITYIDGDQGLLYYRGYPIEQLAEKSDYLEVCYLLIYGELPTPEQKA
                     EFDNTVRRHTMVHEQLTWFFRGFRRDAHPMAMMVGVVGALSAFYQDSLDIANPEHRKI
                     AIYRLISKIPTIAAMCYRYSNGLPFNYPKNNLSYAENFLHMMFATPCEEYKPNPVLAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGASHGGANEAVLKML
                     DEIGDVSRVAEYMEGVKQRKYRLMGFGHRVYRNMDPRASIMRETCYEVLKELGLEDSP
                     KFKLAMELEQIALKDPFFIERKLYPNVDFYSGIVLSALGIPTEMFTVIFALSRSVGWI
                     SHWHEMISDPSLKIGRPRQLYTGSERRDYVLPGER"
     gene            complement(5773..6021)
                     /locus_tag="ACFM8S_000491"
     CDS             complement(5773..6021)
                     /locus_tag="ACFM8S_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HFC6313613.1"
                     /translation="MMVFDDIAKRKIRFQTRRGLLELDLIFGRFMEKEFEHLSDKELS
                     EFSEILEFQDQELLALINGHSETDKGHLIPMLEKIRRA"
     gene            complement(6025..6732)
                     /locus_tag="ACFM8S_000492"
     CDS             complement(6025..6732)
                     /locus_tag="ACFM8S_000492"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (quinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HFC6313614.1"
                     /translation="MEKMSFEIYRYNPDVDAKPYMQRYELELEPTDVKLLDALVRLKA
                     QDDTLSFRRSCREGICGSDGMNINGKNGLACLTDLRGLKQPVKIRPLPGLPVIRDLIV
                     DMTQFFKQYHSVKPYVVNDNPIDADKERLQTQEERKELDGLYECILCACCSTACPSFW
                     WNPDKFVGPSGLLNAYRFIADSRDTITNERLDNLNDPYRLFRCHTIMNCVDVCPKHLN
                     PTRAIGKIKEIMLKRAV"
     gene            complement(6852..8615)
                     /gene="sdhA"
                     /locus_tag="ACFM8S_000493"
     CDS             complement(6852..8615)
                     /gene="sdhA"
                     /locus_tag="ACFM8S_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFC6313615.1"
                     /translation="MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLNCAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHM
                     GMPFDRVESGKIYQRPFGGHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQF
                     FVEWTAQDLIRDENGDVVGVTAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMN
                     TGDGLGICARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNADGERFMERY
                     APTVKDLASRDVVSRAMAMEIYEGRGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISI
                     QFAGIDPIKDPIPVVPTTHYMMGGIPTNYHGEVVVPQGDEYEVPVKGLYAAGECACAS
                     VHGANRLGTNSLLDLVVFGKAAGDSMIKFIKEQSDWKPLPANAGELTRQRIERLDSQT
                     DGENVDALRRELQRSVQLHAGVFRTDEILSKGVREVMAIAERVKRTEIKDKSKVWNTA
                     RIEALELDNLIEVAKATLVSAEARKESRGAHASDDHPERDDENWMKHTLYHSDTNTLS
                     YKPVHTKPLSVEYIKPAKRVY"
     gene            complement(8618..8959)
                     /gene="sdhD"
                     /locus_tag="ACFM8S_000494"
     CDS             complement(8618..8959)
                     /gene="sdhD"
                     /locus_tag="ACFM8S_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HFC6313616.1"
                     /translation="MVERKLTGAHYGLRDWAMQRATAVIMLIYTVALLVVLFSLPKEY
                     SAWQAFFSQTWVKVFTQVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVG
                     CLVYSIKVIWG"
     gene            complement(8953..9330)
                     /gene="sdhC"
                     /locus_tag="ACFM8S_000495"
     CDS             complement(8953..9330)
                     /gene="sdhC"
                     /locus_tag="ACFM8S_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HFC6313617.1"
                     /translation="MSAKPRPVYLDLPNIRLPIPGIVSILHRISGVGLFIMLPFLLYF
                     LSGTLSQESAFETYRAIVSHPLVKLVLIGVLWAYLHHSLAGIRFLFLDAHKGLELNTA
                     RNTAKAVFASALVLTVVLGALLW"
     gene            complement(9609..11012)
                     /locus_tag="ACFM8S_000496"
     CDS             complement(9609..11012)
                     /locus_tag="ACFM8S_000496"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC6313618.1"
                     /translation="MKKIQADVVVIGGGTAGMGAFRNARLHSDNVYLIENNVFGTTCA
                     RVGCMPSKLLIAAAEARHHALHTDPFGVHLDKDSIVVNGEEVMQRVKSERDRFVGFVV
                     ADVEEWPADKRIMGSAKFIDEHTVQIDEHTQITAKSFVIATGSRPVILPQWQSLGNRL
                     IINDDVFSWGTLPKRVAVFGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSD
                     EAKAVFGEELKLHLDAKTEVKLDADGNVEVHWEQDGEKGVFVAEYMLAAVGRRPNVDN
                     IGLENINIEKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAG
                     RYPNIGGGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQADEFVIGEVSFKNQGRSRVM
                     LVNKGHMRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVI
                     EEGLRTALRDADAKLKA"
     gene            complement(11261..11998)
                     /locus_tag="ACFM8S_000497"
     CDS             complement(11261..11998)
                     /locus_tag="ACFM8S_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HFC6313619.1"
                     /translation="MALQDRTGQKVPSVVFRTRVGDTWKDVSTDDLFKGKKVVVFSLP
                     GAFTPTCSSSHLPRYNELFGAFKENGVDAIYCVSVNDTFVMNAWAAEEESDNIYMIPD
                     GNGEFTEGMGMLVGKEDLGFGKRSWRYSMLVNDGVVEKMFIEPEELGDPFKVSDADTM
                     LQFVAPDWKAQESVAIFTKPGCQFCAKAKQALQDKGLSYEEIVLGKDATVTSVRAITG
                     KMTAPQVFIGGKYIGGSEDLEAYLAKN"
     gene            complement(12277..14553)
                     /gene="metE"
                     /locus_tag="ACFM8S_000498"
     CDS             complement(12277..14553)
                     /gene="metE"
                     /locus_tag="ACFM8S_000498"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFC6313620.1"
                     /translation="MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLAVAKDLRE
                     KNWKHQAAANADYVAVGDFTFYDHILDLQVATGAIPARFGFDSQNLSLEQFFQLARGN
                     KDQFAIEMTKWFDTNYHYLVPEFHADTEFKANAKHYVQQLQEAQALGLKAKPTVVGPL
                     TFLWVGKEKGAVEFDRLSLLPKLLPVYVEILTELAAAGAEWIQIDEPALAVDLPQAWL
                     DAYQDVYATLSQVNAKILLSTYFGSVAAHAGRLKALPVAGLHIDLVRAPEQLDAFADY
                     GKVLSAGVIDGRNIWRANLNNVLATLEPLKAKLGDRLWISSSCSLLHTPYDLSVEEKL
                     KVNKPALFSWLAFTLQKTQELRVLKTALNQGREAVAAELADSQAAADSRANSTEIHRA
                     EVAKRLADLPADADQRKSPFAERIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARAAFK
                     KGELSDADYEAAMKKEIALVIEEQEKLNLDVLVHGEAERNDMVEYFGELLEGFAFTQY
                     GWVQSYGSRCVKPPVIYGDVSRPNAMTVAWSTYAQTLTKRPMKGMLTGPVTILQWSFV
                     RNDIPRSTVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLAWAS
                     EAFRLSSTGCEDSTQIHTHMCYSEFNDILPAIASMDADVITIETSRSDMELLTAFGEF
                     KYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPFERLWVNPDCGLKTRGWKETLE
                     QLQVMMNVTRKLRAELAK"
     gene            complement(14691..15569)
                     /gene="metF"
                     /locus_tag="ACFM8S_000499"
     CDS             complement(14691..15569)
                     /gene="metF"
                     /locus_tag="ACFM8S_000499"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFC6313621.1"
                     /translation="MNYAKEINALNNSLSDLKGDINVSFEFFPPKNEQMETMLWDSIH
                     RLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASPDELRQIAK
                     DYWDSGIRRIVALRGDEPPGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKSA
                     QADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTNFKQL
                     GKMAQVTNVKIPSWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYTLN
                     RSELTYAICHILGVRP"
     gene            15769..16044
                     /locus_tag="ACFM8S_000500"
     CDS             15769..16044
                     /locus_tag="ACFM8S_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFC6313622.1"
                     /translation="MKPNIHPDNYRTVLFFDSSANEGWLIRSCAETHGKTMVWTDGKE
                     YPLFSLDTSSASHPVYTGKQRNVNTEGRASKFNQRFQSVMSSFRKDK"
     gene            16044..16169
                     /gene="ykgO"
                     /locus_tag="ACFM8S_000501"
     CDS             16044..16169
                     /gene="ykgO"
                     /locus_tag="ACFM8S_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFC6313623.1"
                     /translation="MQVLSSLKTAKQRHRDCQIVRRRGKVYVICKSNPRFKARQR"
     gene            complement(16275..16475)
                     /locus_tag="ACFM8S_000502"
     CDS             complement(16275..16475)
                     /locus_tag="ACFM8S_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313624.1"
                     /translation="MIGRLFRIFFFFALAALIINRLFSRKQKRALRDVAKISAWVLLG
                     AAAATLFWYLFMLYFKHIPDSY"
     gene            16633..17772
                     /locus_tag="ACFM8S_000503"
     CDS             16633..17772
                     /locus_tag="ACFM8S_000503"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HFC6313625.1"
                     /translation="MSQNASVGIVTPQKIPFEVPLVLENGKTLPRFDLMIETYGELNA
                     EKNNAVLICHALSGNHHVAGRHSAEDKYTGWWDNMVGPGKPIDTERFFVVGLNNLGGC
                     DGSSGPLSINPETGREYGADFPVVTVKDWVKSQAALADYLGIGQWAAVVGGSLGGMQA
                     LQWAISYPERVRHALVIASAPKLSTQNIAFNDVARQAILTDPDFNEGHYRSHNTVPAR
                     GLRIARMMGHITYLAEDGLGKKFGRDLRSNGYQYGYGVEFEVESYLRYQGDKFVGRFD
                     ANTYLLMTKALDYFDPAADFGNSLTRAVQDVQAKFFVASFSTDWRFAPERSHELVKAL
                     IAAQKSVQYIEVKSAHGHDAFLMEDEAYMRAVAAYMNNVYKECQQ"
     gene            17769..18350
                     /gene="metW"
                     /locus_tag="ACFM8S_000504"
     CDS             17769..18350
                     /gene="metW"
                     /locus_tag="ACFM8S_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HFC6313626.1"
                     /translation="MNLRDDLQLIYDRIPEGSRVLDLGCGDGELLAALVEHKKCSGYG
                     IEIDTDSVIAAMSRGVNVIQADLEEGLTAFNDQSFDVIVLSQTIQAMQNTEKILRCLM
                     RVAKQAIVSFPNFGYWRNRVQIALGGHMPVSERMPYHWYDTPNIHWCTLKDFDLLCAK
                     NNIRILERAVMTGNRQVKHFPNLLGSLAFYRVG"
     gene            18467..19312
                     /locus_tag="ACFM8S_000505"
     CDS             18467..19312
                     /locus_tag="ACFM8S_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NMB0938 family lipoprotein"
                     /protein_id="HFC6313627.1"
                     /translation="MKNKTSSLLLWLAAMMLTACSPSKEDKTKENGASAASSTASAAS
                     SSAPQTDLQPAASAPDNVKQAESVPPSNCTDLHPATGIDDLMQQIAEHIDSDCLFALS
                     HHELETRFGLPGGGYDNIQRLLFPDIRPEDPDYHQKIILAIEDLRYGKRTISRQAQDA
                     LMEQERRLREATLLLIQGSQETRGQGEEPKRTRYFEVSATPAYSSRHNNGLGGNFQYI
                     GQLPGYLKIHGEMLENQSLFRLSNRERNPDKPFLDIHFDENGKITRIVVYEKNIYFNP
                     NLGRR"
     gene            complement(19362..19922)
                     /gene="efp"
                     /locus_tag="ACFM8S_000506"
     CDS             complement(19362..19922)
                     /gene="efp"
                     /locus_tag="ACFM8S_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFC6313628.1"
                     /translation="MKTAQELRAGNVFMVGNDPMVVQKTEYIKGGRSSAKVSMKLKNL
                     LTGAASETIYKADDKFDVVILSRKNCTYSYFADPMYVFMDEEFNQYEIEADNIGDALK
                     FIVDGMEDQCEVTFYEGNPISVELPTIIVREVEYTEPAVKGDTSGKVMKTARLVGGTE
                     IQVMSYIENGDKIEIDTRTGEFRKRA"
     gene            complement(19965..21113)
                     /gene="earP"
                     /locus_tag="ACFM8S_000507"
     CDS             complement(19965..21113)
                     /gene="earP"
                     /locus_tag="ACFM8S_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HFC6313629.1"
                     /translation="MNTPPFVCWIFCKVIDNFGDIGVSWRLARVLHRELGWQVHLWTD
                     DVSALRALCPDLPDVPCVHQDIHVRTWHSDAADIDTAPVPDAVIETFACDLPENVLHI
                     IRRHKPLWLNWEYLSAEESNERLHLMPSPQEGVQKYFWFMGFSEKSGGLIRERDYRDA
                     VRFDTGSLRRRLMLPEKNAPEWLLFGYRSDVWAKWLDMWQQAESQMTLLLAGAQIIDS
                     LKQSGIIPQNALQNDGDVFQTASVRLVKIPFVPQQDFDKLLHLADCAVIRGEDSFVRA
                     QLAGKPFFWHIYPQDENVHLDKLHAFWDKAHGFYTPETVSAHRRLSDDLNGGEALSAT
                     QRLECWQILQQHQNGWRQGAEEWSRYLFGQPSASEKLAAFVSKHQKIR"
     gene            complement(21132..21410)
                     /locus_tag="ACFM8S_000508"
     CDS             complement(21132..21410)
                     /locus_tag="ACFM8S_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313630.1"
                     /translation="MSRNLLVRWLVVCLIPLATLAVFVANPPEDKPQHLINGIILACE
                     ATFLFKFVLFDTIKHHLKQEFDLKRQTMLLFIPIVLLVVYLFHYFGAF"
     gene            complement(21421..>21642)
                     /locus_tag="ACFM8S_000509"
     CDS             complement(21421..>21642)
                     /locus_tag="ACFM8S_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313631.1"
                     /translation="FQTDAPGFQYARACCRLVLRHRRQRPIFRGTPVSGVFKGSAVRC
                     FPAARLQTASPARPLDKACAWGDNGVLLF"
BASE COUNT         5355 a         5505 c         5427 g         5355 t
ORIGIN      
        1 aaattcagac ggccttttca ttaacaaata aaaggccgtc tgaaacgatt acagatccaa
       61 caacaggcgg gccgggtctt ccaacgcgtc tttaatggct accaaggtca atacagcttc
      121 gcgaccgtcg atgatacggt ggtcgtaaga cagagccaga tacatcattg gacggacaac
      181 aacttgaccg ttttcaacca cagcgcgctc tttagtggca tgcataccca aaatcgcaga
      241 ttgaggcggg ttgatgatcg gggtagacat catagaaccg aaagtaccgc cgttggtaat
      301 actgaatgta ccgccggtca gatcttcgat agcgattttg ccatctttgg cttttttcgc
      361 gtaatcaaca attgcttgtt cgatgtcggc aatgctcatt tggtcggcat cgcgcaggat
      421 tggcacaacc aaaccgcgtg ggctgccaat cgcgataccg atgtcgaagt agccgtggta
      481 cacgatgtct ttgccgtcaa cagaagcatt cacaaccggg tattttttca gggcggcgac
      541 agcggcttta acgaagaagg acataaagcc caatttcacg ccgtgttctt tctcgaattt
      601 ttctttgtac ttcgcacgca agtccatgat tggtttcatg ttgacttcgt tgaatgtagt
      661 cagaatggcg ttttcttgtt gagaagccaa gaggcgttct gcaacacggg cacgcaggcg
      721 gctcattggt acgcgttctt caggacgtgc gccggcaggg gctgcggctg cggcaggttt
      781 ggcagcggca ttttgtacgt cttctttcaa tacgcgaccg tcacggccgg aaccttgcaa
      841 tgcgttcacg tcaacaccgc tctctgcagc cagtttggcg gcggcaggca tagcggcgtt
      901 gttttgtgca gcagcaggag cggcggccgg ggcagcttcg gcaggagcgg cggcaggtgc
      961 ttcagcagcg gcagtagcag cggcaggtgc ttcagcagcg gcagtagcag cggtatcgat
     1021 acgtgccaaa acttggtcgg caacaacggt ttcaccgtct tgcgctacga tttcaaccaa
     1081 tacgccggct tgtggagaag gtacttccaa aaccactttg tccgtttcga tatcgatcag
     1141 gatttcgtca cgggcaactg cttcgccaac ttttttcttc cattccaaga gcgtgccttc
     1201 agatacgctt tcagacagca taggtacttt tacatcaata atcattttgt gtctccaatg
     1261 gccctttcag acggcctgtt gtgtgttttt cattctgcat atgggtgttc cgcctgagca
     1321 gcctcagacg gaatgctact tggcttacaa tgccaaagcg tcttcaacca attgtttcaa
     1381 ttgagcaatg tgtttgcttg agtagcccac tgcaggcgat gcgctgcttg gacgaccggc
     1441 ataagacagt ttttgctctt cgctgataac gtcttcgatg cggtggcgga tttggtagaa
     1501 cgcgccttgg tttttcggct cttcttgtgc ccaaaccaca gattttgcgt tcggatattt
     1561 cgccagttcg gctttaacct cctcgtatgg gaacggatac agctgctcaa cgcggacgat
     1621 agcgacatca tcttccagtt tacgctcagc acggccggtt tccaagtcat agtaaacctg
     1681 accggcacac aataccacgc gtttcacgct gtcgttgctt gcacgttcgg ctgtatcgcc
     1741 gataaccgga cggaaggtcg aaccttcggt gaagttttcc agcgggctca ttgcaccttt
     1801 gaagcgcaac aggcgtttgg acatgaaaat caccagcggt ttgcggtatg aacccaagac
     1861 ttgacgttgc aagaggtgga acatttgcga cgcttcagac ggcataatga cttgcatatt
     1921 gttctcagaa cacagttgca accaacgttc tacgcgtgca gaagagtgct cagggccttg
     1981 accgtcgtaa ccgtgcggca ggatggtagt cagaccgcac aagcgacccc atttggtttc
     2041 gcctgaagac aggaattgat caatcgtcac ttgcgcgccg ttggcgaaat cgccgaattg
     2101 cgcttcccaa atggtcagct tgtcaggagc agagcaggca aagccgtact cgaacgccat
     2161 cacggcctct tcgttcaaga tggagtcgat aaccaggaac tcgcccattc cttcgcccat
     2221 atggcgcagg ggaacataag taccgtcgtc ccatttttcg cgtttttgat cgtgcaatac
     2281 ggcgtggcgg tgcgagaacg taccgcgacc ggagtcctca ccggagatac gcacgccatg
     2341 acctttggtt acgaggctgg cgtatgccaa ggtttcggcc ataccccaat ctatggcctg
     2401 tttgccggat gccatggctt tacgcgcttc aatcacacgt cttgcagtcg gatgcagggc
     2461 aaagccttcc ggtacggcgg taaacttctc ggtaagacgc tcaatatctg aggcaggcaa
     2521 accggtttcg atgtgttcgc gccaatcttt gccttggtat ttgctccagt cgatttgtgt
     2581 gcgttggaag ttgctcaacg ttgtttgctc gacatgttcg cctttgtcca aagcatcacg
     2641 gtaggcttgg atgtaaccgt cagcctcggc ttgggttacc acgccttcgg caatcagttg
     2701 ttcggtgtac aaagcacgcg cacccgggtg ttgcgatact tttttgtaca tcatcggttg
     2761 ggtcaaggta ggatcgtcgc cctcgttgtg gccccattta cggtagcaga caacgtcgat
     2821 cacgatgtct ttatggaatt ttttgcggta atccaaagca gcttggatag caaagcaaac
     2881 gcgttcggga tcatcgccgt tcacatggat aaccggagcg gaaaccattt tcgcgatatc
     2941 ggtacagtgt acggtcgaac gggtatctcg gatatcggaa gtggtaaagc cgatttggtt
     3001 gttaattacg atgtgaaccg taccgccggt ggtataaccg cgcgttttag acagattgaa
     3061 tgtcgcttgg ttgacaccca aaccgataaa tgcggagtcg ccgtgaatca atacgggcaa
     3121 gactttatcg cggccgtttt cgcccaaacg tttttgcttg gcgcgtgcag aaccttccac
     3181 taccggattg acgatttcca agtgtgacgg gttgaacgcc aaagaaacgt gcatcggacc
     3241 atgtggagtg gcgatgtcgg agctgaagcc catgtggtat ttcacgtcgc cgctaggcaa
     3301 tttgatttcg gcacggcctt caaactcagc gaacaaatct ccgggttttt tacccaaaat
     3361 gttcaccaaa acattcagac ggccacggtg cgccataccg atgatgactt cttccacgcc
     3421 gtctttaccg gcgttttgaa tcaggtagtt caaaccggca atcgcgcttt cgccgccttc
     3481 gacaccgaaa cgtttctgac cgacatattt ggtatgcaga taacgttcca aagtctcggc
     3541 agcagtcatc tctttcaaga tacggcgttt ttgatcggca ttgtaatgcg gtgtggacaa
     3601 tacgctttca aaataattgc gtacccagcg gcgttcttcg gtattgggaa tatagatata
     3661 ttccaatgcg atgtggccgc agtaggtttg tttgaggttg ctgatgattt gggataaagg
     3721 cagtttgccg cgattggcaa aatcgccctc gcccatattg aattggagcg ccatatcggc
     3781 atctgacaga ccgtggaatt tcggatcgag ggcttcaata tcgcgcggcg ggatacgttt
     3841 gagcggatca agttgggctg cacccacgcc ttgtatacga taagcagaaa tcagccgtaa
     3901 aacgctgact tgctttttca gcattgcctc atccgcactg cccgcaacgg cagatgcaat
     3961 tttctttttc gccaaagtaa caaatgattc gcgaatcggt gtgtgtgcga catcgacagc
     4021 aaccgtcccc ggctgtttgc tcaaatcggt gaaatactgc ttccattttt catcaaccgc
     4081 atcggggttt tccaaaaaag cctcgtacaa ttcctcaatg taaggtgcgt ttgaaccgaa
     4141 caggtaagag aaattgagtt tttcgtccat catggcgtgc gctcttttct ttaaaatata
     4201 aacaaagggt agaagtccgc gtcctgacgg ctaaactgat gccgcctgaa aagacatttt
     4261 attctaccct attttaaacc ggattgcttg tcttgatttc atccgactac aatcagacgg
     4321 attcgggaaa caatccggtt ctctgatatt gcctgtttga caatatatta atgtatgtta
     4381 cctctcgcct ggcagcacat aatcgcggcg ttccgaacca gtataaagct ggcgcgggcg
     4441 gccgattttc agcgaaggat cgctaatcat ctcgtgccag tgcgaaatcc agcccacgct
     4501 gcgcgacagg gcgaagatga cggtaaacat ttcggtcggg atgcccagcg cggacaggac
     4561 gatgccggaa tagaaatcga cgtttggata cagtttgcgt tcgataaaga acgggtcttt
     4621 cagcgcaatc tgttccaatt ccatcgccag tttgaatttc ggactgtctt ccaagcccaa
     4681 ttccttcaaa acttcatagc aggtttcgcg cataatgctg gcacgcggat ccatattgcg
     4741 gtacacgcgg tgtccgaagc ccatcaggcg gtatttgcgt tgtttcacgc cttccatata
     4801 ttcggcaaca cgggatacat caccgatttc gtccaacatt tttaagacgg cttcgttcgc
     4861 gccaccatgg gatgcacccc acagacaggc gataccggca gcgatacagg caaatgggtt
     4921 tgcacctgaa gaacctgcca agcgtacggt cgaagtagag gcgttttgct cgtggtcggc
     4981 atgcaaaata aagatgcggt cgagcgcgcg agccaatact ggattaggtt tgtattcttc
     5041 acatggtgtc gcaaacatca tgtgcaagaa gttttcagca taggaaaggt tattttttgg
     5101 ataattgaac ggcagaccgt tggaataacg gtaacacatt gctgcaattg tcgggatttt
     5161 ggagattaag cggtaaatcg caattttgcg gtgctcagga ttggcaatat ccaagctgtc
     5221 ttggtagaac gcagacaaag cgccaaccac gccaaccatc atcgccatcg gatgcgcatc
     5281 gcgacggaac ccccggaaga accaagtcag ctgttcatgc accatcgtgt ggcggcggac
     5341 ggtattgtca aattctgcct tttgctcggg agtcggcagt tcgccgtaaa tcaacaggta
     5401 gcagacttcc aaataatcgg acttttcggc cagctgctcg atggggtatc cgcgataata
     5461 aagcaagcct tgatcgccgt cgatgtaagt aattttagac tcacagcttg cggttgaaac
     5521 aaatccgggg tcgaaggaaa acaaacctgt attttttgtc agcccccgaa tgtcaaccac
     5581 atcgtgcccg atgctggctt ccaataccgg cagctccaaa cctgcctgac ccggtacgtt
     5641 gagtttgatt gatttggaca tattattgct cctttgcaag ttataaccca cgttactgca
     5701 tccgcagcac gttctttgcc ttcagacggc ttttgcattt tgaaatctgc ctgcaggcag
     5761 atttcagatg cctcatgccc gtctgatttt ttcaagcatc gggataaggt gccctttgtc
     5821 cgtttccgaa tgcccgttaa tcaaggcaag caattcttga tcttgaaatt caaggatttc
     5881 ggaaaactcg gacagctctt tatcgctcaa atgctcgaat tctttttcca taaacctgcc
     5941 gaagattaaa tctaactcca acaatccccg gcgggtttga aaacggattt tccgtttggc
     6001 aatatcgtca aaaaccatca tttcttaaac ggcccgtttc aacataatct ctttaatctt
     6061 accgatggct cgggtcggat tcaagtgttt aggacatacg tctacgcagt tcataatggt
     6121 gtggcaacgg aacaaacggt atgggtcgtt cagattatcc aaacgctcat tagtaatggt
     6181 atcacggcta tccgcaatga aacggtaagc attcagcaaa ccagacgggc caacgaattt
     6241 atcagggttc caccagaatg acgggcaggc agtcgaacag caggcgcaca aaatacattc
     6301 gtacaaaccg tccaactctt tacgctcttc ctgagtttgc agacgctctt tgtccgcatc
     6361 aatcggatta tcgttgacaa cataaggttt gacggaatgg tattgtttga agaactgggt
     6421 catatccaca atcaggtcgc ggataacagg cagacctggc agaggacgga ttttaactgg
     6481 ctgtttcaag ccacgcagat cggtcaaaca cgccaagccg tttttgccgt tgatgttcat
     6541 accgtcagaa ccgcaaatcc cttcgcggca ggagcggcgg aaagacaagg tatcgtcttg
     6601 tgctttcagg cgcactaaag cgtccaaaag tttcacgtcg gtcggttcca attccaactc
     6661 gtaacgctgc atataaggct tggcatcaac atccgggttg taacggtaaa tttcaaaact
     6721 cattttttcc atgagaagtg ttccttttct catcaactgc ccaatgattt tgtattataa
     6781 attcaagcag ttcaatgtgg gtatggttag gtttcagacg gcctttgagg taaaggccgt
     6841 ctgaaaacgc atcaataaac gcgcttggcc ggtttgatgt attccacgct caagggcttg
     6901 gtatgcaccg gtttgtaaga caaggtattg gtatctgaat ggtacagcgt atgtttcatc
     6961 cagttttcat catcgcgctc aggatggtcg tctgaagcgt gcgcaccgcg tgattcttta
     7021 cgtgcttcgg cagacaccaa agtcgctttc gccacttcaa ttaggttatc caattccaaa
     7081 gcctcgatac gcgcggtatt ccacactttg ctcttgtctt tgatttcggt acgtttcaca
     7141 cgctcggcaa tcgccatgac ttctcgaacg cctttgctca gaatctcatc agtacggaac
     7201 acgccggcgt gcaattgtac ggagcgttgc agttcgcggc gcaatgcatc aacgttttca
     7261 ccatcggttt ggctgtccaa acgctcgata cgttggcggg tcaactcacc agcattagca
     7321 ggcaaaggtt tccagtcgct ttgctctttg atgaatttaa tcatgctgtc gccggcagct
     7381 ttaccgaata ccaccaagtc cagcagggag ttcgtaccca aacggttcgc accgtgtacg
     7441 gaagcacagg cgcactcacc tgcggcatac aggcctttta caggcacttc gtattcgtct
     7501 ccttgcggca caacgacttc gccgtggtaa ttggtcggaa tgccgcccat catatagtgg
     7561 gtagtcggca caacgggaat cgggtcttta atcggatcga taccagcgaa ctgaatggaa
     7621 atctcgcgga tgcccggcag tttttccata attttttctg cgccgatatg gtcgattttc
     7681 agtaagacat ggtctttgtt tttaccgcaa ccgcgacctt cgtagatttc catcgccatc
     7741 gcgcgggaaa caacgtcgcg agaagccaag tcttttacgg tcggcgcata gcgttccata
     7801 aagcgttcgc cgtcggcatt caacagaata ccgccctcgc cgcgtacgcc ttcggtaatc
     7861 aacacgcccg cacctgccac gccggtcggg tggaattgcc agaattccat gtcttccaac
     7921 gggatacctg cacgcgcaca aatacccaaa ccatcgccgg tattcatata ggcattggta
     7981 gaagacgcat aaatacggcc gccgccgccg gtagcaaaca tcacagcttt agcgtggaaa
     8041 atataaactt cgccggtttc catttccatg gcggttacgc cgacgacatc gccgttttca
     8101 tcacgaatca aatcttgtgc cgtccattcc acaaagaatt gcgtattggc acggacgttt
     8161 tgttggtaca aagtatgcag catcgcatga cctgtacggt cggcaaccgc acaggcgcgt
     8221 tctaccgcgc gtttaccgtg ttcggcagta tggccgccga aaggacgctg ataaatttta
     8281 ccgctttcca cacggtcaaa aggcataccc atgtgttcca actcaattac ggcttcaggc
     8341 gcggcgcggc acataaactc aatcgcatct tggtcgccca accagtcgga acctttcacg
     8401 gtatcgtaca tgtgccagtc ccaacggtct tcctgcacat tacccagaga ggcggaaata
     8461 ccgccctgcg ccgctacggt atgcgaacgg gtcgggaaca ctttagacaa aacggcacaa
     8521 ttcagaccgg atttggataa ttggagggct gcgcgtaaac ctgcaccacc accgccgaca
     8581 atcacggcat caaacttgcg aacaggaaaa cccatactta cccccaaatt actttaattg
     8641 aatacaccaa gcagccgacc agccagacga tggtggcaac ctgcaaaaac aaacgcacgc
     8701 cgaagggttt gatatagtcc atccacaaat cgcggatacc cacccaagcg tgcaagaata
     8761 cggcgatgaa gctcacttgg gtaaatactt ttacccaagt ttgactaaaa aatgcctgcc
     8821 atgccgaata ttctttaggc agggaaaata gaactactaa aagtgcaacg gtataaatca
     8881 acataataac cgcagtcgca cgttgcatcg cccaatcgcg caaaccgtaa tgggcaccgg
     8941 tcaatttacg ttctaccata acaacgctcc caaaacgaca gtcaaaacca atgcagaagc
     9001 aaatacggct ttagcggtat tgcgcgcagt attcagctca aggcctttgt gtgcatccaa
     9061 aaataaaaag cggataccgg cgagagaatg gtgcagataa gcccacaata caccgattaa
     9121 aaccagcttg accaaaggat gggaaacaat ggcacggtaa gtttcaaatg cagactcttg
     9181 actcagggta ccggacagga aatacagcag gaaaggcagc ataataaaca gcccgacccc
     9241 gctgatgcgg tgaaggatgg aaactatccc gggtatcggc agacggatgt tcggcaaatc
     9301 cagataaaca ggacgcggtt tggcagacat agtcaattcc tctgtagtta tatatcgact
     9361 caaaaaccgt aaacccattg ctacactggg ttacataaac gttaatttac ctgttttatc
     9421 ccattttgaa cagtgtattt taaaaacaaa ctgatagatt ttgacaaaca cgcgtggaaa
     9481 cagcagatta aaagaaagat ttttagtttt gccgccccct tcccgacacg gtatcagcat
     9541 ccgaccccat ccccaaaaac aaaatgccgt ctgaaaaaat ttcagacggc attgttttgc
     9601 catatcggtc aggctttcaa tttcgcatcg gcatcgcgca acgcggtacg cagaccttcc
     9661 tcgataacgg gatggtagaa cggcatatcc agcatttgcg gaacggtcat cttcatttga
     9721 tgcgcccatg ccaacaagtg cgccaaatgt tcggcggcag ggcctacgat ttccgcgccg
     9781 ataaagcggc cggtggcttt ttcggcatac aggcgcatat ggcctttgtt caccagcatc
     9841 acgcggctgc ggccttggtt tttgaacgat acttcgccga tgacaaattc gtcggcttgg
     9901 tattgcgcgg caacctgcgc gtatttcaga ccgacaaagc cgatttgcgg actggtaaac
     9961 accacgccga tggtgctgcg ccgcaaaccg ccgccgatat tcgggtaacg gcctgcattg
    10021 tcgccggcaa tcttgccttg gtcggcagct tcgtgcagca ggggcagttg gttggacgcg
    10081 tcgcccgcaa taaagatatg cggaatgctg gtctgcatgg tcagcgggtc ggcaacgggt
    10141 acgccgcgcg catctttttc gatattgata ttttccaaac cgatattgtc aacgttcgga
    10201 cggcggccca cggctgccag catatattcg gcaacaaata cgcctttttc gccgtcctgc
    10261 tcccaatgga cttctacatt gccgtctgca tcgagcttga cctcggtttt agcatccaaa
    10321 tgcagtttca attcttcgcc gaacacggct ttcgcctcgt ctgaaacgac ggggtcggaa
    10381 atgccgccga tgattccgcc caaaccgaaa atttcaactt tcacgcccaa acggtgcaat
    10441 gcctgaccca gttccaaacc gataacaccc ggcccgaaca cggcaacgcg cttaggcagc
    10501 gtaccccatg agaaaacgtc atcgttgata atcaaacgat tgcccaaaga ctgccattgc
    10561 ggcaggatga cgggacgcga accggtagca atcacgaaac tttttgccgt aatttgagta
    10621 tgctcgtcga tttggacggt atgctcgtcg ataaatttag ccgaacccat gatgcgcttg
    10681 tcggcaggcc actcttccac atcggcaacg acaaagccga caaaacggtc acgctcggat
    10741 ttgacgcgct gcatgacctc ttcgccgttg acgacgatgc tgtctttgtc caaatgcacg
    10801 ccgaacgggt cggtatgcaa tgcgtgatga cgcgcctctg cggcggcaat caagagtttg
    10861 gaaggcatac agcccacgcg cgcgcaggtc gtgccgaaca cattgttttc aatcaggtaa
    10921 acattatccg aatgtaaacg ggcattgcga aacgcaccca taccggcagt accgccgccg
    10981 attacgacga catccgcttg aatttttttc ataatctttg tccttccgtt ttcgtctgaa
    11041 acattgccga cacagaacat atcggtttca gacggcattt aaatattttc ccaatcaaaa
    11101 acatcattca gataaataaa aacaaaattt cgattgagtg caacaatatc caaattctct
    11161 tataaatata gagttattcg gagcggcagt gtagaaccgc ccgaataact ctatccggta
    11221 tatcagacag tttaatcaaa ccgccttaag caaacagcta tcagttttta gccaagtaag
    11281 cttccaaatc ttcgctgccg ccgatgtatt tgccgccgat gaagacttga ggggcagtca
    11341 tcttgccggt aatggcgcga acggaagtaa cggttgcatc tttgcccaat acgatttctt
    11401 cgtaagacaa acctttgtct tgcaaagctt gtttggcttt agcgcagaat tggcaacctg
    11461 gtttagtgaa aattgccaca gactcttgag ccttccaatc gggagcaacg aattgcagca
    11521 tagtatctgc atcggatact ttgaacggat cgcccagttc ttcaggttcg atgaacattt
    11581 tttcaaccac gccgtcgtta accagcatgg agtaacgcca agagcgttta ccgaagccca
    11641 agtcttcttt accgaccagc atacccatac cttcggtaaa ttcgccgttg ccgtcaggaa
    11701 tcatgtagat gttgtcggat tcttcttcgg cagcccaagc gttcattacg aacgtatcgt
    11761 ttacagatac gcagtagatt gcgtcaacgc cgttttcttt gaacgcgccg aacaattcgt
    11821 tgtaacgcgg caggtgtgaa gaagaacaag tcggggtaaa tgcaccgggc agggagaata
    11881 cgactacttt tttgcctttg aacaaatcat cggtagacac atctttccaa gtgtcgccga
    11941 cgcgggtgcg gaatactacg gaaggtactt tttgaccggt acgatcttgc aaagccattt
    12001 taagctcctt aatagattgg tttgagaaat aattgtgaat cgctgggcat tctaccccat
    12061 gcaaaatgat ttgtataatt tatagttcaa ataatataaa ttatttaaaa ctatcaaaca
    12121 caactccgat ttgcctgttt cccacatata aagccgaatt agaaaaaaac aagctgcaat
    12181 gaaaagattt tccggcaact gcgcccttgc gccgcaaatc aggacaaaat accgtctgaa
    12241 aggctttcag acggtattta ttcatacggt ctcggcttat ttcgccaatt cggcacgcag
    12301 ttttcgggtt acgttcatca tcacttggag ttgttccaga gtttctttcc agccgcgtgt
    12361 tttcaggccg cagtctgggt taacccacag acgttcaaac ggtacaacct cgatggcttt
    12421 gcgcaacagg tgctcaactt cggcttcagt cggtacgcgc gggctgtgga tgtcgtaaac
    12481 gcccgggccg atgtcgttcg ggtatttgaa ttcgccgaat gcggtcaaca attccatgtc
    12541 agaacgtgaa gtttcgatgg taatcacgtc cgcgtccatg ctggcgatgg ccggcaggat
    12601 gtcgttgaat tccgagtaac acatatgggt gtggatttgg gtactgtctt cgcaaccggt
    12661 tgacgacagg cggaaggctt cgcttgccca agccaagtag gcatcccaat cggcacgttt
    12721 caacggcagg ccttcgcgga tggccggttc gtcgatttga atcactttaa taccggcttt
    12781 ttccaaatcc aatacttcgt catttaaggc caaggcgatt tgtttgcaca cggtgctgcg
    12841 cggaatatcg ttgcgcacaa atgaccattg cagaatcgtt accggaccgg tcaacatgcc
    12901 tttcatcggg cgtttggtca gggtttgggc gtaggttgac caagccacag tcatcgcgtt
    12961 cgggcggcta acatcgccgt aaatcaccgg tggtttcacg cagcgtgagc catagctttg
    13021 tacccagccg tattgggtaa aggcaaagcc ttccagcaat tcgccgaaat attccaccat
    13081 gtcgttacgc tcggcttcgc cgtgtaccaa cacatccaaa ttgagttttt cttgctcttc
    13141 aatcaccaag gcgatttctt ttttcatcgc ggcttcgtaa tcagcgtcag acaattcgcc
    13201 ttttttgaac gcggcacgcg cttggcggat ttcagtggtt tgcgggaacg aaccgatatt
    13261 ggtggtcggc agcaacggca ggttcagcca tgcttgttgc gctttaatgc gctcggcaaa
    13321 cggtgatttg cgttggtcgg catctgccgg taaatcagcc aagcgtttgg ccacttcggc
    13381 gcggtggatt tcggtgctgt tggcgcgtga atcagcagcg gcttggctgt cggccaattc
    13441 ggcggcaacg gcttcgcggc cttggttcaa tgcagttttc aacacgcgca attcttgcgt
    13501 tttctgcaag gtgaaagcca accatgagaa caaggcaggt ttgttgactt tcagcttttc
    13561 ttctaccgac aaatcgtatg gcgtgtgcag cagcgagcat gagctggaaa tccacaagcg
    13621 gtcgcccaat ttggctttca acggctcaag cgtggctaat acgttgttta aattggcgcg
    13681 ccaaatatta cggccatcaa tcacaccggc agacaacact ttgccgtaat cagcaaatgc
    13741 gtccaattgc tcgggcgcgc gcaccaaatc gatgtgcaaa ccggccacag gcaaggcttt
    13801 cagacgacct gcgtgtgcgg caaccgaacc gaaataagtg ctcaacaaga ttttggcatt
    13861 cacttggctc aaagtggcgt aaacgtcttg gtacgcatcc aaccacgctt gcggcaggtc
    13921 aaccgccaat gctggctcat cgatttgaat ccattcggca ccggcagcgg ccaattcggt
    13981 caggatttca acataaaccg gcaataattt tggcaacagg ctcaagcggt caaattcgac
    14041 agcgcctttt tctttaccaa cccacaggaa ggtcaacgga ccaacaacgg tcggtttggc
    14101 tttcaggccc aaagcttggg cttcttgcag ctgttgaacg tagtgtttgg cgttcgcttt
    14161 gaattcggta tcggcgtgga attcaggcac caagtagtgg tagttggtgt cgaaccattt
    14221 ggtcatttcg atagcgaatt ggtctttatt gccgcgtgcc agttggaaga attgttccaa
    14281 agacaggttt tggctgtcga agccgaaacg ggcagggatg gcaccggtag cgacttgcag
    14341 gtcgaggatg tggtcgtaga aagtgaaatc gcctacagca acgtaatcag cgttggcagc
    14401 ggcctggtgt ttccagtttt tctcgcgcaa gtctttagca acagccagca attcttgctc
    14461 gctgatttct ttgcgccagt atttttcttg tgcgaatttc aattcgcgga aggcgccgac
    14521 acgcgggaag cctgagaaat gtaatgttgt catgttgaac tctccagttg gattttgtaa
    14581 ggaccggttc aatccgaatc ggccggtatt gtaaatcaga atggctcgga tggggcggtg
    14641 ttttgctttt tagacacctt agatgccgtc tgaaactgtt tgatacggct ttaagggcgc
    14701 acgcctaaaa tatggcaaat ggcgtaagtc agctcgctgc ggttaagcgt atagaagtgg
    14761 aaatctttca cgccttcgcg ggacagaact ttgaccatat cgatggcgat acttgccgcc
    14821 accagattgc gcgtaccttg gtcgtcgtcc aaaccttcat acatttgcga cagccagctt
    14881 gggattttga cgttggttac ttgagccatt ttaccgagct gcttgaagtt ggtaacaggc
    14941 aaaataccgg ggacgatttc cacatcgatg cccaacatca cgcagcggtc gcggaagcgc
    15001 aggtagcgtt ccacatcgaa gaagaattgg gtgatgacgt ggttcgcgcc cgcatcgatt
    15061 ttgcgtttca aattaatcag gtcggcttgt gcggatttcg cttcgggatg cacttcggga
    15121 tatgctgcta cagaaatgtc gaagtcggcg acggagcgta atagcttaac caagtcttcg
    15181 gcataaaacg gttttttctc gtaaccgggc ggctcgtcgc cgcgcagggc gacaatgcgg
    15241 cggatgccgc tgtcccaata atctttggca atttggcgca attcgtcggg agaagcgtcg
    15301 atgccggtca ggtgcggcgc ggcttccaag ccggtttctt gcttaatgcg tttgacgatg
    15361 ccgtgcgtgc ggtcgcgttc gccggaattt gcgccgtaag ttacggatac gaacttggga
    15421 tgcagggttt gcagacggtg gatggagtcc cacagcatcg tttccatttg ttcgtttttc
    15481 ggcgggaaaa attcaaacga aacgttaatg tcgcctttca aatcagaaag actgttattt
    15541 aacgcattga tttcttttgc gtaattcatg gctgcgtacc ttttattatc ttttatatga
    15601 tgtttcaggc aacataataa agttgcgcca aacatgagtc aatttcaaaa ttttcagatt
    15661 tatcatgaaa atttctaata tattacaaaa atagttaaat ttccttgtac aagccttcta
    15721 atttatgata aagtttgtta tgttataaca attcaactaa ggcgatatat gaaaccgaat
    15781 attcatcctg acaattatcg aaccgtcctt tttttcgaca gcagcgccaa tgaaggctgg
    15841 ttgattcgtt cttgcgccga aacacacgga aaaacgatgg tttggacaga cggcaaagaa
    15901 tatccgctgt tttcgctgga tacttcttcc gcatcccatc ccgtctatac tggcaaacag
    15961 cgcaacgtca acaccgaagg ccgtgccagc aaattcaacc aacgtttcca atccgtcatg
    16021 tcatcgttta gaaaggacaa ataatgcagg ttctgtcttc actcaaaact gccaaacaac
    16081 gccaccgcga ctgccaaatc gtcagacgca ggggcaaagt ttacgtcatc tgcaaaagca
    16141 atccgcgttt taaagcgcgc caacgctgac cccctaccct ttcctgcttc ggttgtaaag
    16201 cccctcctac cgcaagccct tttcaaatat gccgtctgaa acagaaaaat gcgtttcaga
    16261 cggcattttt tccgtcaata cgaatccgga atgtgtttga aatacagcat aaacagatac
    16321 caaaacagtg tcgcggcagc tgcgccaagc agtacccatg cgctgatttt ggcgacatcg
    16381 cgcagggcac gtttttgctt gcggctgaag aggcggttga taatcagcgc ggcaagtgcg
    16441 aaaaagaaaa aaatacggaa aagtctgcct atcatatagg gaagcggtgg aggtcgtctg
    16501 aaaaagtatg gatcggttgc ggcggcatgg ggacggatgt ttcagcgcgt cgggctgctt
    16561 gtccgaccgt gtcaaaaagt catgccgttt gctatataat aatgcttttc gccgcatttg
    16621 ttggaacagg atatgagtca aaatgcctcg gtgggcattg taacgcccca aaaaattccg
    16681 tttgaagtgc cgctggtttt ggaaaacggt aaaactttgc cgcgtttcga tctgatgatt
    16741 gaaacctacg gcgagctgaa tgctgaaaaa aacaatgcgg ttttaatctg ccacgcgctg
    16801 tcgggcaacc atcatgttgc gggcaggcat tcggcggagg ataaatatac gggctggtgg
    16861 gacaatatgg tcggacccgg caaaccgatt gatacggaac gttttttcgt ggtcggtttg
    16921 aacaatctgg gcggctgtga cggcagcagc ggacctttgt cgatcaatcc tgaaacgggt
    16981 agggaatacg gcgcggattt tccggtggtt acggtgaagg actgggtaaa atcacaagcc
    17041 gcgcttgccg actatctcgg catcgggcaa tgggcggcgg ttgtcggcgg cagtttgggc
    17101 ggtatgcagg ctttgcagtg ggcgatttcc tatcccgaac gcgtgcgcca tgccttagtg
    17161 attgcgtctg cgccgaaact gtccacgcaa aatatcgcgt tcaacgatgt ggcgcgtcag
    17221 gcgattttga ccgaccccga tttcaacgaa ggacattacc gcagccataa taccgttcct
    17281 gcacggggct tacggattgc ccgcatgatg gggcacatta cctatcttgc cgaagacggt
    17341 ttgggcaaaa aattcggacg cgatttgcgt tccaacggct atcaatacgg ttatggcgtt
    17401 gaatttgaag tagaatccta tctgcgctat cagggcgaca aattcgtcgg gcggtttgat
    17461 gccaacacct atttgctgat gaccaaagct ttggactatt tcgatccggc ggcggatttc
    17521 ggcaacagcc tgacccgcgc cgtgcaggat gtgcaggcaa aattctttgt cgccagcttc
    17581 agcaccgatt ggcgtttcgc gcccgaacgt tcgcacgaac tggtcaaggc cctgattgcc
    17641 gcccaaaaat ccgtgcagta tatcgaagtc aaatccgcac acgggcacga tgccttttta
    17701 atggaagacg aagcctatat gcgtgcggtc gccgcctata tgaacaacgt ttataaggaa
    17761 tgtcagcaat gaacctgcgc gatgatttgc aactgattta cgaccggata cccgaaggca
    17821 gccgcgtctt ggacttgggc tgcggcgacg gcgaactgct tgccgccttg gtcgaacaca
    17881 aaaaatgcag cggctacggc atcgaaatcg acaccgacag cgtgattgcc gcgatgtcgc
    17941 gcggcgtgaa tgttatccaa gccgatttgg aagaaggttt aaccgcattc aacgatcaaa
    18001 gttttgatgt gattgtgttg agccaaacca tccaagcgat gcagaatacc gaaaaaatcc
    18061 tgcgctgcct gatgcgcgtt gccaaacagg cgattgtcag cttcccgaat ttcggctact
    18121 ggcgcaaccg cgtccaaatc gcactcggcg gacatatgcc ggtttccgaa cgtatgccct
    18181 accattggta tgatacgccg aacatccatt ggtgtacgct caaagacttt gatttattgt
    18241 gcgccaaaaa caacatccgc atcctcgagc gcgccgtgat gacgggcaac agacaggtca
    18301 aacatttccc caaccttttg ggcagtcttg cattttaccg cgtcgggtaa gacagattga
    18361 gaaccttgtc ggcattaaca ggttcttcgg ttctattaac aacaggccgt ctgaaaaatg
    18421 aaacggtagg ttttcagacg gcctctttaa tactggaaac aatagaatga aaaacaaaac
    18481 ctcatcactt ctcttatggc ttgccgcaat gatgctgacc gcgtgttccc cgagcaaaga
    18541 agataaaacg aaagaaaacg gcgcatccgc cgcctcgtcc acggcatccg ccgcttcgtc
    18601 ttccgcgccc caaaccgatt tgcaaccggc cgcatccgcc cctgataacg tcaagcaggc
    18661 agaaagcgtg ccgccgtcaa attgcaccga cctgcacccc gccaccggca ttgacgatct
    18721 catgcagcaa atcgccgaac acattgactc ggactgtctg tttgcccttt cccatcacga
    18781 actggaaacc cgtttcggct tacccggcgg cggctatgac aacatacagc ggctgctgtt
    18841 tcccgacatc cgccctgaag atcccgacta ccatcagaaa atcatactgg caattgaaga
    18901 cttgcgttac ggaaagcgca cgatcagccg gcaggcacaa gatgccttga tggaacagga
    18961 acgccgcctc cgagaagcga cgctgttgct gatacagggc agtcaagaaa cccgcggaca
    19021 aggcgaggag ccgaaacgca cgcgttattt tgaagtttcg gcaacccctg cctattcgag
    19081 ccggcacaac aacggacttg gcggcaattt ccaatacatc ggccaattgc ccggctatct
    19141 gaaaatacac ggagaaatgc ttgaaaacca atcactcttc cggctgtcca accgtgaacg
    19201 caatcccgac aaaccgtttt tagacatcca ttttgacgaa aatggcaaaa tcacgcgtat
    19261 tgtcgtttac gaaaaaaaca tctacttcaa tccaaacttg gggcgaagat aaaaaaatgc
    19321 cgtctgaaac gttttcagac ggcatttttc aatgcaacaa atcaagcgcg tttgcggaat
    19381 tcgccggtgc gggtatcgat ttcgattttg tcgccgtttt cgatgtaaga catcacttgg
    19441 atttcggtgc ccccgaccag gcgcgcggtt ttcatcactt tgcctgaagt atcgcctttg
    19501 acggcaggct cggtatactc gacttcgcgt acgatgatgg tgggcagttc tacggagatg
    19561 gggttgcctt cgtagaaggt aacttcgcat tggtcttcca taccgtcaac gatgaatttc
    19621 aacgcgtcgc cgatgttgtc ggcttcgatt tcgtattggt tgaattcttc gtccataaag
    19681 acgtacatcg gatcggcaaa gtagctgtac gtacagtttt tgcgggacag gatgaccacg
    19741 tcgaatttgt cgtcggcttt gtaaatggtt tcggaagccg cgccggtcag caggtttttc
    19801 agtttcatgc tgactttggc ggaagagcgg ccgcctttga tgtattcggt tttttgaacg
    19861 accataggat cgttgccgac cataaataca ttgccggcgc gcagttcttg tgctgttttc
    19921 attgtgattt tccaatcaaa tcggttgtcg taaaacgcgc tattctagcg tattttttga
    19981 tgctttgaaa caaaggcggc gagtttttcg gatgcggaag gctgcccgaa aagataacgg
    20041 ctccactcct ctgcgccttg ccgccagccg ttttgatgtt gttgcaggat ttgccaacat
    20101 tcgaggcgtt gtgttgcgga taaagcctct ccgccgttga ggtcgtccga aagacggcgg
    20161 tgtgccgaca cggtttcggg cgtgtagaaa ccgtgtgcct tatcccaaaa ggcgtggagt
    20221 ttgtcgagat ggacattctc gtcttgcgga tagatgtgcc aaaagaaggg ttttccggca
    20281 agctgggcgc gcacgaaact gtcttcgccg cggatgacgg cgcagtcggc aaggtgcagc
    20341 agtttgtcga agtcctgttg cggcacgaaa gggattttga cgaggcggac ggatgccgtc
    20401 tgaaaaacat cgccgtcgtt ttgcagggcg ttttgcggaa tgatgccgct ttgtttgagg
    20461 ctgtcgataa tttgcgcccc cgccagcagc agggtcatct ggctctctgc ctgttgccac
    20521 atatccagcc actttgccca aacatcgctc cggtagccga aaagcagcca ttcgggggcg
    20581 tttttttcgg gaagcatcag ccgccgacgc agggatccgg tatcgaaacg gacggcatct
    20641 cggtaatcac gttcgcgtat caacccgccg cttttttcgc tgaaacccat aaaccaaaaa
    20701 tatttttgaa caccctcctg cggcgaaggc ataagatgca gcctttcatt gctttcctcc
    20761 gcgctcaaat attcccaatt cagccaaagc ggcttgtgtc ggcggataat gtgcagcaca
    20821 ttttcgggca ggtcgcaggc aaaagtttcg atgacggcat cgggaacagg cgcggtatca
    20881 atatctgccg catcggaatg ccaagtgcgg acatgaatat cctgatgaac gcagggaaca
    20941 tcgggcaaat cagggcaaag cgcacgcaag gcggacacat cgtccgtcca caaatgcacc
    21001 tgccaaccga gttcgcggtg caaaacacgg gcaagccgcc acgaaacgcc gatgtcgccg
    21061 aaattgtcga tgaccttgca aaaaatccaa cagacaaaag gaggagtatt catagaaata
    21121 acggaaacaa gctaaaacgc gccgaaatag tggaacaaat acacaaccag caaaacaatc
    21181 ggaataaaca gcaacatggt ttgacgtttc aaatcaaact cttgtttcaa atgatgcttg
    21241 atggtgtcga aaaggacgaa tttgaacaaa aacgtcgctt cgcaggcaag gatgatgccg
    21301 ttgatcagat gctggggttt gtcttcgggc ggattggcga cgaaaacggc aagcgtcgcc
    21361 aaagggatga ggcagacgac aagccagcgg acaagcaggt ttcgggacat tgcaaggctt
    21421 tcaaaaaagc aaaacgccat tatcgcccca agcgcacgcc ttgtctaacg gacgcgccgg
    21481 cgatgccgtc tgaagccttg ccgcaggaaa acaccgcacc gccgaacctt taaatacgcc
    21541 cgagacgggc gttccccgaa aaatcggacg ctgccgccga tgcctgagga caaggcggca
    21601 gcaggcacgg gcatattgaa aacccggcgc gtcggtttga aa
//
LOCUS       DAXHSF010000017        20931 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.017, whole genome shotgun sequence.
ACCESSION   DAXHSF010000017 DAXHSF010000000
VERSION     DAXHSF010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20931
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.017"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(79..771)
                     /locus_tag="ACFM8S_000510"
     CDS             complement(79..771)
                     /locus_tag="ACFM8S_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFC6313632.1"
                     /translation="MFHAFILRIYTMIKNISKLINHAVLIGFIPNIVFAYPIFAENPA
                     SYQNIHVRSWKARRDSGIVKQDLDFSCGAASIATLLNNFYGRHYSEAEILDKMDKTQM
                     RASFDDMQRIMPELGFEAQGYALPFEQLVQLKIPVIVYLKYRKNNHFSVLNGINGETV
                     LLADPSLGHVSMSKSQFLSAWKTRDGEMEGKILAIVPKNTDFVRNQMFFNKNPVRQTR
                     FTVEQIQMRQKR"
     gene            complement(1077..1634)
                     /locus_tag="ACFM8S_000511"
     CDS             complement(1077..1634)
                     /locus_tag="ACFM8S_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313633.1"
                     /translation="MMQKHKCLIDFILTVASQIAYADLPLSLEELLTDKGKFKLESSI
                     SYINTERNQSEFANPIYVQTSATKIATVMAMMITPVMAQNLDSQVFDSQNVKAIQLSQ
                     AEMKETQGEFVPIIAAAAFGGALGAWEYHGANLYNHGKLGTAQGAATAAGIGAATGVA
                     AKQGWLLLPEADWLEIWHGDRVFMH"
     gene            complement(1879..2037)
                     /locus_tag="ACFM8S_000512"
     CDS             complement(1879..2037)
                     /locus_tag="ACFM8S_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313634.1"
                     /translation="MKPPLPICTLSALAACTLSGQGGTQVYGEIKARDLCVTAPILLS
                     AFCLACSR"
     gene            complement(2034..2876)
                     /locus_tag="ACFM8S_000513"
     CDS             complement(2034..2876)
                     /locus_tag="ACFM8S_000513"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC6313635.1"
                     /translation="MLVCNPYEVVIHGTTSSGKIFRPSDWAERLCGILSSFTKDHRLS
                     YSKWVRPMLVDNIRCVAVDKKLETDNPQMFRFLMDFAADNDLRVIDCKALLEEREQGG
                     QNNPADEHVMLAQAIEEKHAAEKAQEQTASGASYVLREIGADDTATAFAALSVLRSAL
                     TDINRFTEQINKVQRPQGYRLLGIFEEGKHNAVAVCGFREACTLASGRHIHIDDIVTL
                     PQSRRKGYASRLLEEVRKIGAETGVTKIHLNVHVNHDRADAHRLYFKNGFEICAYHFR
                     CDPK"
     gene            3115..4035
                     /gene="pyrB"
                     /locus_tag="ACFM8S_000514"
     CDS             3115..4035
                     /gene="pyrB"
                     /locus_tag="ACFM8S_000514"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFC6313636.1"
                     /translation="MPNPLYRQHIISISDLSREQLECLLQTALKLKAHPRGDLLEGKL
                     IGSCFFEPSTRTRLSFETAVQRLGGKVIGFSDGANTSAKKGETLADTARIISGYTDAI
                     IQRHPKDGAARVAAEFSRVPVINAGDGTNQHPSQTLLDLVTIYETQGRLDKLKIAMAG
                     DLKYGRTVHSLCQALKRWGCEFAFVSPPSLAMPDYITEELDEAGCRYRILGSLEEAAE
                     WADILYMTRVQRERFDEQEFAKIQGKFNLEASMLAHAKPNLRVLHPLPRVDEIHPDVD
                     ATPHAYYFEQATNGVYARMAILSLVLNEEV"
     gene            4045..4503
                     /gene="pyrI"
                     /locus_tag="ACFM8S_000515"
     CDS             4045..4503
                     /gene="pyrI"
                     /locus_tag="ACFM8S_000515"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFC6313637.1"
                     /translation="METPKLSVEAIEKGTVIDHIPAGRGLTILRQFKLLHYGNAVTVG
                     FNLPSKTQGSKDIIKIKGVCLDDKAADRLALFAPEAVVNTIDNFKVVQKRHLNLPDEI
                     AEVFRCPNTNCAGHGEPVKSRFYVKKHNGQTRLKCHYCEKTYSRDSVAEA"
     gene            4568..5233
                     /locus_tag="ACFM8S_000516"
     CDS             4568..5233
                     /locus_tag="ACFM8S_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313638.1"
                     /translation="MMMHASVQSRFAPILYVLIFFAGFLTAQIWFNQKAYTEELPPLL
                     SALSAVALVWLAWAFVSARSKAKAEKFYREKMIQNESIHPVLHASLQHLEHKPQMLAL
                     LVKNHGKGMAEQVRFKAEVLPDDEDARTIAAELAKMDMFALGTDAVASGETYGRVFAD
                     IFELSAALEGRAFKGMLKLTAEYKNIFGDACRSETALELGALNQALQEISKTSEKSKR
                     IFY"
     gene            complement(5296..6513)
                     /locus_tag="ACFM8S_000517"
     CDS             complement(5296..6513)
                     /locus_tag="ACFM8S_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFC6313639.1"
                     /translation="MQRRIITLLCAAGMAFSTQTLAANLEVRPNAPERYTVKQGDTLW
                     GISGKYLYSPWQWGRLWDANRDQIHNPDLIYPDQVLVLRHVDGEPRLGLEQTDGIPVV
                     KMSPDKEVSGYGIPAIDVNFYRIFMRHPQIVSRKETAAAPRLLSGPEGRLLYTKGTRV
                     YTKGLKEPGRYLTYRINKNITDPDTGKFLGQEVAFSGIVRSLDYTDSVLEQRSKQAGE
                     RPKDNEYHTRTHPLITPLRTPSIQPLVVETAISEIQQGDYLMKMPEDTDRFNMMPHEP
                     SRPVQAKIVSVFEGTRIAGQFQTITIDKGEADGLDKGTVLSLYKRKKTMQVDLSNNFK
                     SRDTVELISTPAEEVGLAMVYRTSEHLSSAIILENISDISVGDTAANPGRDLDNIPDQ
                     GRSRVKFGFNRSE"
     gene            6697..7200
                     /gene="def"
                     /locus_tag="ACFM8S_000518"
     CDS             6697..7200
                     /gene="def"
                     /locus_tag="ACFM8S_000518"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFC6313640.1"
                     /translation="MALLNILQYPDERLHTVAKPVEQVDERIRKLIADMFETMYESRG
                     IGLAATQVDVHERVVVMDLTEDRSEPRVFINPVIVEKDGETTYEEGCLSVPGIYDTVT
                     RAERVKVEALNEKGEKFTLEADGLLAICVQHELDHLMGIVFVERLSQLKQGRIKTKLK
                     KRQKHTI"
     gene            7287..8213
                     /gene="fmt"
                     /locus_tag="ACFM8S_000519"
     CDS             7287..8213
                     /gene="fmt"
                     /locus_tag="ACFM8S_000519"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFC6313641.1"
                     /translation="MKVIFAGTPDFAAAALKAVAAAGFEIPLVLTQPDRPKGRGMQLT
                     APPVKQAALELGLRVAQPEKLRNNAEALQMLKEVEADVMVVAAYGLILPQEVLDTPKH
                     GCLNIHASLLPRWRGAAPIQRAIEAGDAETGVCIMQMDIGLDTGDVVSEHRYAIQPTD
                     TANEVHDALMEIGAAAVVADLQQLQSKGRLNAVKQPEEGVTYAQKLRKEEARIDWSES
                     AAVIERKIRAFNPVPAAWVEYQGKPMKIRRAEVVAQQGAAGEVLSCSADGLVVACGEN
                     ALKITELQPAGGRRMNIAAFAAGRHIEAGTKL"
     gene            8292..9551
                     /gene="rsmB"
                     /locus_tag="ACFM8S_000520"
     CDS             8292..9551
                     /gene="rsmB"
                     /locus_tag="ACFM8S_000520"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFC6313642.1"
                     /translation="MSMALAQKLAADSIAAVAEGRNLQDVLAQIRTAHPDLTAQENGA
                     LQDIAYGCQRYLGSLKHMLAQMLKKPIDNPQLESLLLAALYQLHYTRNAPHAVVNEAV
                     ESIAKIGRGQYRSFANAVLRRFLREREKLASSCKKDDVAKHNLPLWWVAYLKNHYPKH
                     WHNITAALQSHPPMTLRVNRRHGNAESYLEKLAAEGIPARALDEYAVTLEEAVPVNRL
                     PGFSDGIVSVQDFGAQQAAYLLNPKDGERILDACAAPGGKTGHILELADCRVTALDID
                     AGRLKRVEDNIARLGFQTASAACADARDLAAWYDGKPFDAILADVPCTASGVARRNPD
                     VKWLRRPTDALKTARQQEALLDALWQTLKQGGRMLLATCSVFVEENDGQLQKFLNRHA
                     DAEPIESRVLLPNKHQDGFYYALIQKH"
     gene            9517..10116
                     /locus_tag="ACFM8S_000521"
     CDS             9517..10116
                     /locus_tag="ACFM8S_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HFC6313643.1"
                     /translation="MAFITRLFKSIKQWLVLLPMLSVLPDAAAEGIDVSRAEARITDG
                     GQLSISSRFQTELPDQLQQALRRGVPLNFTLSWQLSAPIIASYRFKLGQLIGDDDNID
                     YKLSFHPLTNRYRVTVGAFSTDYDTLDAALRATGAVANWKVLNKGALSGAEAGETKAE
                     IRLTLSTSKLPKPFQINALTSQNWHLDSGWKPLNIIGNK"
     gene            10116..12236
                     /locus_tag="ACFM8S_000522"
     CDS             10116..12236
                     /locus_tag="ACFM8S_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC6313644.1"
                     /translation="MRRFLPIAAICAVVLLYGLTAATGSTSSLADYFWWIVAFSAMLL
                     LVLSAVLARYVILLLKDRRDGVFGSQIAKRLSGMFTLVAVLPGVFLFGVSAQFINGTI
                     NSWFGNDTHEALERSLNLSKSALNLAADNALGNAVPVQIDLIGAASLPGDMGRVLEHY
                     AGSGFAQLALYNAASGKIEKSINPHKLDQPFPGKARWEKIQRAGSVRDLESIGGVLYA
                     QGWLSAGTHNGRDYALFFRQPVPKGVAEDAVLIEKARAKYAELSYSKKGLQTFFLATL
                     LIASLLSIFLALVMALYFARRFVEPVLSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTK
                     LFNHMTEQLSIAKEADERNRRREEAARHYLECVLDGLTTGVVVFDEQGCLKTFNKAAE
                     QILGMPLTPLWGSSRHGWHGVSAQQSLLAEVFAAIGAAAGTDKPVHVKYAAPDDAKIL
                     LGKATVLPEDNGNGVVMVIDDITVLIHAQKEAAWGEVAKRLAHEIRNPLTPIQLSAER
                     LAWKLGGKLDEQDAQILTRSTDTIVKQVAALKEMVEAFRNYARSPSLKLENQDLNALI
                     GDVLALYEAGPCRFAAELAGEPLMVAADTTAMRQVLHNIFKNAAEAAEEADVPEVRVK
                     SEAGQDGRIVLTVCDNGKGFGREMLHNAFEPYVTDKPAGTGLGLPVVKKIIEEHGGRI
                     SLSNQDAGGACVRIILPKTVETYA"
     gene            12229..13506
                     /locus_tag="ACFM8S_000523"
     CDS             12229..13506
                     /locus_tag="ACFM8S_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HFC6313645.1"
                     /translation="MRSSDILIVDDEIGIRDLLSEILQDEGYSVALAENAEEARKLRH
                     QARPAMVLLDIWMPDCDGITLLKEWAKNGQLNMPVVMMSGHASIDTAVEATKIGAIDF
                     LEKPISLQKLLSAVENALKYGAAQTETGPVFDKLGNSAAIQEMNREVGAAVKCASPVL
                     LTGEAGSPFETVARYFHKNGTPWVSPARVEYLIDMPMELLQKAEGGVLYVGDIAQYSR
                     NIQAGIAFIVGKAEHRRVRVVASGSRAAGSDGIACEEKLAELLSESVVRIPPLRMQHE
                     DIPFLIQGIACNVAESQKIAPAAFSEEALAALTRYDWPGNFDQLQSVVATLLLEADGQ
                     EIGAAAVSSLLGQNVPAEGAEDMVGGFNFNLPLRELREEVERRYFEYHIAQEGQNMSK
                     VAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE"
     gene            13597..14784
                     /gene="dprA"
                     /locus_tag="ACFM8S_000524"
     CDS             13597..14784
                     /gene="dprA"
                     /locus_tag="ACFM8S_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFC6313646.1"
                     /translation="MTEDERFAWLQLAFTPYIGAESFLLLMRRFGSAQNALSAPAEQV
                     APAVRHKQAAEAWRNAEKRALARQAAEAALEWEMRDGCRLMLLQDEDFPEMLTQGLTA
                     PPVLFLRGNVRLLHKPSAAIVGSRHATPQAMRIAKDFGKSLGGQNIPVVSGMASGIDT
                     AAHQGALEAEGGTIAVWGTGIDRIYPPSNKNLAYEIAEKGLIVSEFPIGTRPYAGNFP
                     RRNRLIAALSQVTLVVEAALESGSLITAGLAAEMGREVMAVPGSIDNPHSKGCHKLIK
                     DGAKLVECLDDILNECPGLLQNTGASSYSINKDTPDTGRRAVQTAYAPPPAAKMPSEA
                     AAGGTAHGGILDKMGFDPVHPDVLAEQLAMPAADLYAALLELELDGSVAAMPGGRYQR
                     IRT"
     gene            14810..15271
                     /locus_tag="ACFM8S_000525"
     CDS             14810..15271
                     /locus_tag="ACFM8S_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HFC6313647.1"
                     /translation="MTEVIAYLIEHFQDFDTCPPPEDLGMLLEEAGFDTMEIGNTLMM
                     MEVLLNSSEFSAEPADSGALRVYSKEETDNLPQEVMGLMQYLIEEKAVSCEQREIIIH
                     ALMHIPGDEITVDTAKVLTLLLLWANKSELPVLVGDELMSALLLDNKPTMN"
     gene            15343..17649
                     /gene="topA"
                     /locus_tag="ACFM8S_000526"
     CDS             15343..17649
                     /gene="topA"
                     /locus_tag="ACFM8S_000526"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFC6313648.1"
                     /translation="MAKNLLIVESPSKAKTLKKYLGGDFEILASYGHVRDLVPKSGAV
                     DPDNGFAMKYQLISRNGKHVDAIVAGAKEAENIYLATDPDREGEAISWHLLEILKSKR
                     GLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICERENEIRAFEAQEYWTVHLDSHKGRSKFTAKLAQYNGA
                     KLEQFDLSNEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAPFTTSTMQQDAVRKLGF
                     TTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLP
                     SAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTP
                     AKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESNDDEESEDSKKLPEMSEGDKLP
                     VDKLYGEQHFTTPPPRYNEATLVKALEEYGIGRPSTYASIISTLKDREYVTLEQKRFM
                     PTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIADGKRQWIPLMDKFWKPFIKQVE
                     EKEGIERAKFTTQELDETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEE
                     AAERIAKAEAEQAELDGRECPKCGGRLVYKYSRTGSKFIGCANYPKCKHVEPLEKPKD
                     TGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAEECPNCHWPVLTIKT
                     TKRWGVEKVCPQKECGWKEQIEPPAPKE"
     gene            complement(17722..18417)
                     /locus_tag="ACFM8S_000527"
     CDS             complement(17722..18417)
                     /locus_tag="ACFM8S_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313649.1"
                     /translation="MMKDLNLSNSLFKGYNDKHGLMICGYEWGWSKADEAAYVAGEYK
                     LPENKIDHTFANKSLYFGEQAKKWRYDNTIKNWFEMWGHPLDENGLGGAFEKSLVQTN
                     WAATQGNTIDNPDKFTQPEHIDNFLYHIEKLRPKVILFMGSRLADFLNNQNVLPRFEQ
                     LVGKQTKPLETVQKEFDGTRFNVKFQSFEDCEVVCFPHPSASRGLSYDYIALFVPEMN
                     RILSDFKTTRGFK"
     gene            19108..19485
                     /locus_tag="ACFM8S_000528"
     CDS             19108..19485
                     /locus_tag="ACFM8S_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFC6313650.1"
                     /translation="METHEKIRLMRELNKWSQEDMAEKLAMSAGGYAKIERGETQLNI
                     PRLEQLAQIFKIDMWDLLKSGGGGMVFQINEGDSGGDIALYASGDVSMKIEFLKMELK
                     HCKEMLEQKDKEIELLRKLTETV"
     gene            19574..20143
                     /gene="rsmD"
                     /locus_tag="ACFM8S_000529"
     CDS             19574..20143
                     /gene="rsmD"
                     /locus_tag="ACFM8S_000529"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFC6313651.1"
                     /translation="MAAGKHTKHSNRVRIIGGQCRGRKLSFTSADGLRPTPDSVREKL
                     FNWLGQDLTGKTVLDLFGGSGALGIEAASRNAKRVLISDNNRQTVQTLQKNSRELGLG
                     QVQIVFSDGIAYLKTVSEQFDVVFLDPPFAWQDWQILFDALKPCLNPRAFVYLEAGTL
                     PNIPDWLTEYREGKSGQSTFELRVFQVAE"
     gene            20266..20517
                     /locus_tag="ACFM8S_000530"
     CDS             20266..20517
                     /locus_tag="ACFM8S_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HFC6313652.1"
                     /translation="MSLFITDECINCDVCEPECPNDAISQGEEIYEINPNLCTQCVGH
                     YDEPQCQQVCPVDCILIDEEHPETHDELMAKYEKIIQFK"
     gene            20614..20697
                     /locus_tag="ACFM8S_000531"
     tRNA            20614..20697
                     /locus_tag="ACFM8S_000531"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20648..20650,aa:Tyr,seq:gta)
     gene            20728..20801
                     /locus_tag="ACFM8S_000532"
     tRNA            20728..20801
                     /locus_tag="ACFM8S_000532"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20760..20762,aa:Gly,seq:tcc)
     gene            20811..20885
                     /locus_tag="ACFM8S_000533"
     tRNA            20811..20885
                     /locus_tag="ACFM8S_000533"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20843..20845,aa:Thr,seq:ggt)
BASE COUNT         5233 a         5317 c         5702 g         4679 t
ORIGIN      
        1 gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccactat ataaaataaa
       61 tgggcaaaaa tcggtttatt atcgtttttg ccgcatttgg atttgttcta ccgtaaaacg
      121 tgtttgacga acgggattct tattaaaaaa catctgattt ctaacaaaat cagtattttt
      181 tggcacgatg gctaaaattt ttccttccat ttcgccatca cgtgttttcc atgcgctcaa
      241 gaattgtgat ttgctcattg agacgtgccc cagcgatgga tcagccagca aaacagtttc
      301 tccgttaata ccgttcaata ccgaaaaatg gttgttttta cggtatttta aatacacaat
      361 tacaggaatt tttagttgta ccaactgttc aaatggcaaa gcataacctt gtgcttcaaa
      421 acccagttcg ggcattatgc gttgcatatc atcaaaagaa gcacgcattt gggttttatc
      481 cattttgtcc aagatttccg cttcagaata atgtctgcca taaaaattat tcagtaacgt
      541 ggcaatcgaa gccgcgccgc aagaaaaatc caaatcttgt tttactatgc cggaatctcg
      601 ccgtgctttc caactccgta catggatgtt ttggtaagaa gcggggtttt cagcaaatat
      661 agggtaagca aaaacaatat ttgggataaa gccaattaaa accgcatggt ttattaattt
      721 agaaatattt tttatcatag tatatatgcg tagaataaac gcatgaaaca taaaaaaata
      781 aaatcatatg caattttatt gcatgaataa atatgaataa aatagataat gatgggagta
      841 aatacgccat gtattttgga agtttaaatt tattaataat aaaataattt atcgtagcgc
      901 aaataaatcc caaaattgga acgattagaa aaaaaattaa tacacccatc atcatgcaaa
      961 ctctatatta ataaatagct aactaattct attgtggaaa ttagttagct aactaaaagt
     1021 tattaatgat tatttccgag aattgactgc attgttggca gcattggccc caaaacctag
     1081 tgcatgaata cccggtctcc atgccaaatt tccagccaat ccgcctccgg cagcagcagc
     1141 cagccctgtt ttgccgctac tcctgttgcc gcaccgattc ctgccgcagt agccgcgcct
     1201 tgcgcagttc ctaatttacc atgattatac aaattagcac catgatactc ccatgcacct
     1261 aatgcaccgc caaaagcagc ggctgcaata atgggaacaa attcaccttg tgtttctttc
     1321 atttcagcct gtgataattg aattgctttc acattttggc tgtcaaaaac ttggctgtct
     1381 aaattttgcg ccattacagg tgtaatcatc atagccatta cagttgcaat tttcgttgcg
     1441 ctggtttgca cataaatagg attagcaaat tcgctttgat tgcgttcagt gttgatgtag
     1501 ctaatactgc tttctagttt gaatttaccc ttgtcagtca ataattcttc caaacttaaa
     1561 ggtaagtcag cataagcaat ttggctggca acagtcaaaa taaaatctat tagacatttg
     1621 tgtttttgca tcatttcgtt tgattttcta gtttttgaga atgatacaaa gtttttttac
     1681 aaagtaaaga gtcactctga aaaacatttt ttcattataa atcaaaatat tgatagaata
     1741 aatagcgagc atcgattcac ggtgtacttt agtgcaaagc gtacaacgcg agcctgaacc
     1801 accagccgca acaggaaaag aaagccgata gagtgcaatg cttgccaacg tgcaagcgag
     1861 cttgcaagaa cgcttggctc aacgagagca ggcaagacag aaagcagaaa gcaggatagg
     1921 agcggtaacg caaaggtctc gggctttgat ttcgccgtaa acctgagtgc cgccttgtcc
     1981 ggaaagggtg caggcggcga gtgccgacag ggtgcagatg gggagggggg gtttcatttg
     2041 gggtcgcaac ggaagtggta tgcgcagatt tcaaaaccgt ttttgaaata caggcggtgc
     2101 gcgtcggcac ggtcgtggtt gacgtggacg ttgaggtgga ttttggttac ccctgtttcc
     2161 gcgccgattt tgcggacttc ttccaaaagg cgcgaggcgt agcctttgcg gcggctttgc
     2221 ggcagggtaa cgatgtcatc gatgtggatg tggcggccgc tggcgagggt gcaggcttcg
     2281 cggaagccgc agacggcgac ggcattgtgt ttgccttctt caaaaatacc cagcaggcgg
     2341 tagccttggg ggcgttggac tttgttgatc tgttcggtaa agcggttgat gtcggtcagg
     2401 gcggaacgca aaacgctcaa ggctgcaaag gcggtggcgg tgtcgtccgc gccgatttcg
     2461 cgcaaaacgt aggatgcgcc cgaggcggtc tgttcctgtg ctttctcggc ggcgtgtttt
     2521 tcttcgattg cctgtgccag catgacgtgt tcgtcggcag ggttgttttg tccgccctgt
     2581 tcgcgttctt cgagcagggc tttgcagtcg atgacgcgca ggtcgttgtc ggcggcaaag
     2641 tccatcagga agcggaacat ttgggggttg tcggtttcca gttttttatc gacggcgacg
     2701 cagcggatgt tgtccaccag catggggcgc acccatttcg aataggaaag cctgtggtct
     2761 ttggtgaacg aggacagaat gccgcacagg cgttccgccc agtcgctggg acggaaaatc
     2821 ttgccggaac tcgttgtgcc gtggatgacg acttcgtagg ggttgcagac taacatggcg
     2881 gcttcctgaa aagaaatgtc tggcgcgatt ataccttatg cttatgcggg cgtgtttgga
     2941 tatgccgtct gaaaagtacg ggattcgtgc ggtaaaactt tgcggcggca aatgtgcgat
     3001 aatacgcgcc gtattgccgc ttttgcgaag ctgttccgca aacatacggg cggcgtggac
     3061 gacgtataac cggatacccg cctgacgcgg gttttttacg gaaggggggc aaaaatgcct
     3121 aatccgcttt acagacagca tatcatctcc atttcggatt tgtcgcgcga acagttggaa
     3181 tgcctgcttc agacggcatt gaagctgaag gcgcatccgc gcggcgattt gttggaaggc
     3241 aaacttatcg gttcgtgctt ctttgaaccg tccacgcgca cgaggctgtc gtttgaaacg
     3301 gcggtgcagc ggctgggcgg caaggtcatc ggcttttcgg acggcgcgaa taccagtgcc
     3361 aaaaaaggcg agacgcttgc cgataccgcc cgcatcattt ccggctacac cgacgcaatc
     3421 atccaacgcc accccaaaga cggcgcggcg cgcgtggcag cggagttttc gcgcgtcccc
     3481 gtcatcaacg ccggcgacgg caccaaccag caccccagcc agaccttgtt ggatctggta
     3541 acgatttatg aaacgcaggg gcgtttggac aagctcaaaa tcgccatggc gggcgacttg
     3601 aaatacgggc gcaccgtgca ttcgctttgt caggcgttga aacgctgggg ctgcgaattt
     3661 gcctttgttt cgccgcccag cctcgcgatg cccgactaca ttaccgaaga gttggacgaa
     3721 gccggctgcc gataccgtat cctcggcagt ttggaagagg cggcggaatg ggcggacatc
     3781 ctgtatatga cccgcgtcca gcgcgaacgt ttcgatgaac aggaatttgc caaaatccaa
     3841 ggcaaattca acctcgaagc gtctatgctc gcccacgcca aaccgaacct gcgcgtgctg
     3901 caccctttgc cgcgcgtgga cgaaatccat cccgatgtcg atgccacgcc gcatgcctat
     3961 tatttcgagc aggcaaccaa cggcgtttat gcgcgtatgg cgatattgtc gctggtgttg
     4021 aacgaagaag tgtgaggaac cgatatggaa accccgaaac tcagtgtcga agccattgaa
     4081 aaaggtacgg ttatcgacca tattcccgcc ggcagggggc tgaccatcct gcgccagttc
     4141 aaacttttgc actacggcaa cgcagtaacc gtgggcttca acctgcccag caaaacccaa
     4201 ggcagcaaag acatcatcaa aatcaaaggc gtgtgcttgg acgacaaagc cgccgaccgc
     4261 cttgccctgt ttgcacccga agcagtggtc aacaccatcg acaatttcaa ggttgtgcag
     4321 aaacggcatt tgaacctgcc cgacgaaatc gccgaagtgt tccgctgtcc gaacacgaat
     4381 tgcgccggcc acggcgagcc ggtcaaaagc cggttttatg ttaaaaaaca caacgggcag
     4441 acgcggctga aatgccatta ctgcgaaaaa acctacagcc gggattcggt ggcggaagcc
     4501 tgacggattc ccttaaaccg agcgggcggc atttcgtctg ccgcttgttt tgccaatctg
     4561 aaatggaatg atgatgcacg cttctgtcca aagccgtttc gcaccgatac tttatgtttt
     4621 gattttcttt gccggttttt tgaccgcgca aatctggttc aatcagaaag cctatactga
     4681 agagctgcct ccgcttctgt ccgcattgtc cgctgtcgcg ctggtgtggc tggcgtgggc
     4741 gttcgtgtcg gcgcgttcaa aggctaaggc ggaaaagttc taccgcgaaa aaatgataca
     4801 gaacgaaagc atacatcccg tcctgcacgc ttctttgcaa cacttggaac acaagccgca
     4861 aatgctcgcc ctgctggtca aaaaccacgg caaagggatg gcggaacagg tcaggttcaa
     4921 ggcggaagtg ctgcccgacg acgaagacgc gcgcacgatt gccgccgagt tggcaaaaat
     4981 ggatatgttt gcattgggga cggacgcggt cgcctcgggc gaaacctatg gacgcgtgtt
     5041 cgccgatatt ttcgagttgt cggcggcttt ggaagggcgc gcgttcaaag gaatgttgaa
     5101 actgacggcg gaatataaaa acatcttcgg cgatgcctgc cgttcggaaa cggcgttgga
     5161 gttgggcgcg ctcaatcagg cgttgcagga gatttcaaaa acatcggaaa agtccaaacg
     5221 gatattttat tgaagatgga aaaatgccgt ctgaaacgga aggtgtttca gacggcattt
     5281 ttgtcggatg attaattatt cggagcggtt gaagccaaac ttcacgcggc tgcggccctg
     5341 atccggtata ttgtccaaat cgcgtcccgg attggcggcg gtgtcgccta cggaaatatc
     5401 ggagatgttt tccaaaatga tggcggacga caggtgttcg gaggtgcggt aaaccattgc
     5461 caagcccact tcttcggcag gagtggaaat cagctcgacg gtatccctgc ttttgaaatt
     5521 gttggagagg tcgacctgca tcgttttctt gcgtttgtag aggctcaaaa ccgtgccttt
     5581 gtccaaaccg tccgcctcgc ctttgtcgat ggtgatggtt tgaaactggc cggcaatcct
     5641 tgtgccttca aacacggaaa cgattttagc ctgaaccggg cgggacggtt cgtgcggcat
     5701 catgttgaag cggtcggtgt cttccggcat tttcatcagg tagtcgccct gctgtatttc
     5761 ggaaatggcg gtttcgacca ccagcggctg tatcgaaggg gtgcgcagcg gggtaatcaa
     5821 aggatgggtg cgggtatggt attcgttgtc tttcggccgt tctccagcct gtttcgagcg
     5881 ttgttcgagg acagagtcgg tatagtcgag ggagcgcacg atgccgctga atgcgacttc
     5941 ctgcccgagg aatttacccg tatccggatc ggtgatgttt ttattgattc ggtaggtcag
     6001 gtagcggccc ggctctttca ggcctttggt gtaaaccctg gtgcctttgg tgtacagcag
     6061 cctgccttcc gggcccgaga gcaggcgcgg cgcggcagcg gtttctttgc gggaaacgat
     6121 ttgcgggtgc cgcataaaga tgcggtagaa gttgacatcg atggcgggaa taccgtatcc
     6181 ggacacttcc ttatccggac tcattttgac gacggggatg ccgtctgtct gttccaagcc
     6241 gaggcgcggt tcgccgtcaa cgtggcgcaa caccaatacc tggtccggat aaatcaggtc
     6301 gggattgtgg atttgatccc ggttcgcgtc ccacaggcgg ccccattgcc acgggctgta
     6361 caggtatttg cccgaaatgc cccacagggt gtcgccctgt ttgaccgtgt agcgttccgg
     6421 cgcgttcggg cgcacctcca aatttgccgc caaagtttgt gttgagaatg ccatacctgc
     6481 cgcgcagagc agggttataa tacgacgttg cataaccgtt ccccttatct gataaatttc
     6541 ggtttgtctt gcttgattgg gttggaaaaa gcggcggcag cccctcggga tgtgccgcgt
     6601 gataaaaaat gttccgcatt ttaacatcga attatccgca ccatcacggt aattatgaaa
     6661 aacaggcggc gtatccgccg aaggaaagag aaaattatgg ctttattgaa tatcttgcaa
     6721 tatcccgacg agcgtctgca cacggtggca aagcctgtcg aacaagtcga cgagcgcatc
     6781 cggaagctga ttgccgatat gtttgaaacg atgtacgaat cgcgcggcat cgggctggcg
     6841 gcgacgcagg tcgatgtgca cgagcgcgtg gtcgtgatgg atttgaccga agaccgcagc
     6901 gaaccgcgcg tgttcatcaa ccccgtcatc gttgaaaaag acggcgaaac cacttacgaa
     6961 gagggctgcc tgtccgtgcc gggcatttac gacaccgtaa cccgcgccga acgcgtcaag
     7021 gtcgaggctt tgaacgaaaa aggcgaaaag ttcacgctgg aggcggacgg cttgctggcg
     7081 atttgcgtgc agcacgagtt ggaccacctg atgggcatcg tgtttgtcga acgcctgtcg
     7141 cagctcaagc agggacgcat caaaaccaag ctgaaaaaac gtcagaaaca tacgatttga
     7201 cccttttgcc gtgccgtctg aacgctgcaa agttttcaga cggcacggtc ttgtccgaca
     7261 accttacgca tacgcaggaa tatgctatga aagtcatctt tgccggcacg cccgattttg
     7321 ccgccgccgc cttaaaagcc gttgccgccg ccggttttga aatcccgctg gtgctgaccc
     7381 agcccgaccg tccgaaaggg cgcggtatgc aactgaccgc cccgcccgtc aaacaagccg
     7441 cgctggaact cggtttgcgc gtggcacagc ccgaaaaact gcgtaacaac gccgaagccc
     7501 tgcaaatgct caaagaggtc gaggcagacg taatggtggt tgccgcctac ggtttgattc
     7561 tgccgcagga agtgttggat acgccgaaac acggctgcct caacatccac gcttcgctgt
     7621 taccccgttg gcgtggcgcg gcgccgattc aacgcgcgat tgaagccggc gatgccgaga
     7681 caggcgtgtg tattatgcag atggacatcg gtttggacac cggcgatgtg gtcagcgaac
     7741 accgctacgc catccaaccg accgataccg ccaacgaagt ccacgacgcg ctgatggaaa
     7801 tcggtgcggc ggcggttgtt gccgatttgc aacagcttca aagcaaaggc cgtctgaacg
     7861 cggtcaaaca gcccgaagaa ggtgttactt acgcccaaaa attgcgcaaa gaagaggcgc
     7921 gtatcgattg gagcgaaagc gcggcggtta tcgaacgcaa aatccgcgcc ttcaaccccg
     7981 tgcctgccgc gtgggttgag tatcagggca agccgatgaa aatccggcgg gcggaagtgg
     8041 tggcgcaaca aggcgcggca ggcgaagtgt tgtcctgttc ggcggacggt ttggtcgttg
     8101 cctgcggcga aaacgcgctg aagattaccg aattgcagcc tgccggcggc aggcgtatga
     8161 atatcgcggc gtttgcagca ggacggcata tcgaagcagg gacgaagctg taaacctttt
     8221 tcagacggca tttgatccgc aaacgggaat gccgcctgaa accatcagtt gaagaaagcg
     8281 aatcacataa tatgagtatg gcacttgccc aaaaacttgc cgccgacagc attgcggcgg
     8341 ttgccgaagg acgcaacctt caggacgtgt tggcgcaaat ccgcaccgcg catcccgacc
     8401 ttacggcgca ggaaaacggc gcgttgcagg acatcgccta cggctgccag cgttatttgg
     8461 gcagtttgaa acatatgctc gcgcagatgc tgaaaaagcc gattgacaac ccgcagctcg
     8521 aaagcctgct tttggcggcg ttgtaccaac tgcattacac gcgcaacgcg ccgcacgccg
     8581 tggtcaatga ggcggtggaa agcatcgcga aaatcgggcg cgggcagtac cgttcgtttg
     8641 ccaacgcggt tttgcgccgt tttttgcgtg aacgtgaaaa attggcgtct tcctgcaaaa
     8701 aagacgatgt ggcaaaacac aacctgccgc tgtggtgggt ggcttacttg aaaaaccatt
     8761 atccgaaaca ctggcacaac atcaccgccg cgctgcaatc gcatccgccg atgacgttgc
     8821 gcgtcaaccg ccgacacggc aatgccgaaa gctatttgga aaaactggct gcggaaggta
     8881 ttccggccag ggcgttggac gaatatgcgg ttacgttgga agaagccgtg ccggtaaacc
     8941 gtctgcccgg tttttcagac ggcattgttt cggtacagga cttcggcgcg cagcaggcgg
     9001 cgtatctcct taacccgaaa gacggcgaac ggattttgga cgcgtgcgcc gcgccgggcg
     9061 gcaagacggg gcatatcttg gaactggcgg attgccgcgt taccgccttg gacatcgatg
     9121 caggccgtct gaaacgggtg gaggacaata tcgcccgttt gggctttcag acggcatcgg
     9181 cggcgtgtgc cgatgcgcgg gatttggcgg cgtggtatga tggaaagcct ttcgacgcaa
     9241 tccttgccga cgtgccatgc accgcttccg gcgtggcgcg gcgcaatccc gacgtgaaat
     9301 ggctgcgccg tccgaccgac gcgctcaaaa ccgcccgcca gcaggaagcc ctgctggacg
     9361 cactttggca gacgctgaaa cagggcggca gaatgctgct tgccacctgt tccgtgttcg
     9421 tcgaagaaaa cgacgggcaa ttgcaaaaat tcctcaaccg ccatgccgat gccgaaccga
     9481 tcgaatcgcg ggtgctttta ccgaacaaac accaagatgg cttttattac gcgcttattc
     9541 aaaagcatta aacaatggct tgtgctgctg ccgatgcttt ccgttttgcc ggacgcggcg
     9601 gcggagggga tagatgtgag ccgcgccgaa gcgaggataa ccgacggcgg gcagctttcc
     9661 atcagcagcc gcttccaaac cgagctgccc gaccagctcc aacaggcgtt gcgccggggc
     9721 gtgccgctca actttacctt aagctggcag ctttccgccc cgataatcgc ttcttatcgg
     9781 tttaaattgg ggcaactgat tggcgatgac gacaatattg actacaaact gagtttccat
     9841 ccgctgacca accgctaccg cgttaccgtc ggcgcgtttt cgacagacta cgacaccttg
     9901 gatgcggcat tgcgcgcgac cggcgcggtt gccaactgga aagtcctgaa caaaggcgcg
     9961 ctgtccggtg cggaagcagg ggaaaccaag gcggaaatcc gcctgacgct gtccacttca
    10021 aaactgccca agccttttca aatcaatgca ttgacttctc aaaactggca tttggattcg
    10081 ggttggaaac ctctaaacat catcgggaac aaataatgcg ccgttttcta ccgatcgcag
    10141 ccatatgcgc cgtcgtcctg ttgtacggac tgacggcggc aaccggcagc accagttcgc
    10201 tggcggatta tttctggtgg attgttgcgt tcagcgcaat gctgctgctg gtgttgtccg
    10261 ccgttttggc acgttatgtc atattgctgt tgaaagacag gcgcgacggc gtattcggtt
    10321 cgcagattgc caaacgcctt tccgggatgt ttacgctggt tgccgtactg cccggcgtgt
    10381 ttctgttcgg cgtttccgca cagttcatca acggcacgat taattcgtgg ttcggcaacg
    10441 atacccacga ggcgcttgaa cgcagcctca atttgagcaa gtccgcattg aatctggcgg
    10501 cagacaacgc cctcggcaac gccgtccccg tgcagataga cctcatcggc gcggcttccc
    10561 tgcccgggga tatgggcagg gtgctggaac attacgccgg cagcggtttt gcccagcttg
    10621 ccctgtacaa tgccgcaagc ggcaaaatcg aaaaaagcat caacccgcac aagctcgatc
    10681 agccgtttcc aggtaaggcg cgttgggaaa aaatccaacg ggcgggttcg gtcagggatt
    10741 tggaaagcat aggcggcgta ttgtacgcgc agggctggct gtcggcgggt acgcacaacg
    10801 ggcgcgatta cgccttgttt ttccgtcagc cggttcccaa aggcgtggca gaggatgccg
    10861 tcttaatcga aaaggcaagg gcgaaatatg ctgagttgag ttacagcaaa aaaggtttgc
    10921 agaccttttt cctggcaacc ctgctgattg cctcgctgct gtcgattttt cttgcactgg
    10981 tcatggcact gtatttcgcc cgccgtttcg tcgaacccgt cctatcgctt gccgaagggg
    11041 cgaaggcggt ggcgcaaggc gatttcagcc agacgcgccc cgtgttgcgc aacgacgagt
    11101 tcggacgctt gaccaagttg ttcaaccaca tgaccgagca gctttccatc gccaaagaag
    11161 cagacgagcg caaccgccgg cgcgaggaag ccgcccgcca ctacctcgag tgcgtgttgg
    11221 atgggctgac cacgggcgtg gtggtgtttg acgaacaagg ctgtctgaaa accttcaaca
    11281 aagcggcgga acagattttg gggatgccgc ttacccccct gtggggcagc agccggcacg
    11341 gttggcacgg cgtttcggcg cagcagtccc tgcttgccga agtgtttgcc gccatcggcg
    11401 cggcggcagg tacggacaaa ccggtccatg tgaaatatgc cgcgccggac gatgccaaaa
    11461 tcctgctggg caaggcaacc gtcctgcccg aagacaacgg caacggcgtg gtaatggtga
    11521 ttgacgacat caccgttttg atacacgcgc aaaaagaagc cgcgtggggc gaagtggcga
    11581 agcggctggc acacgaaatc cgcaatccgc tcacgcccat ccagctttcc gccgaacggc
    11641 tggcgtggaa attgggcggg aagctggatg agcaggatgc gcaaatcctg acgcgttcga
    11701 ccgacaccat cgtcaaacag gtggcggcat tgaaggaaat ggtcgaagca ttccgcaatt
    11761 atgcgcgttc cccttcgctc aaattggaaa atcaggattt gaacgcctta atcggcgatg
    11821 tgttggcatt gtatgaagcc ggtccgtgcc ggtttgcggc ggagcttgcc ggcgaaccgc
    11881 tgatggtggc ggcggatacg accgccatgc ggcaggtgct gcacaatatt ttcaaaaatg
    11941 ccgccgaagc ggcggaagaa gccgatgtac ccgaagtcag ggtaaaatcg gaagcggggc
    12001 aggacggtcg gattgtcctg acggtttgcg acaacggcaa agggttcggc agggaaatgc
    12061 tgcacaacgc cttcgagccg tatgtaacgg acaaaccggc gggaacggga ttgggtctgc
    12121 ccgtggtgaa aaaaatcatt gaagaacacg gcggccgcat cagcctgagc aatcaggatg
    12181 cgggtggcgc gtgtgtcaga atcatcttgc caaaaacggt agaaacttat gcgtagcagc
    12241 gatattttaa ttgtagacga cgaaatcggc atccgcgacc tgctgtcgga aatcctgcag
    12301 gacgaaggtt attcggtcgc attggcggaa aacgccgaag aggcgcgcaa gctgcgccat
    12361 caggcgcgcc ccgcgatggt gctgctggat atttggatgc ctgattgcga cggcatcacc
    12421 cttttgaagg agtgggcgaa aaacgggcag ctcaatatgc cggtggtgat gatgagcggg
    12481 catgccagca tcgataccgc cgtggaagcc accaaaatcg gcgcgatcga ttttttggaa
    12541 aaaccgattt ccctgcaaaa gctgctgtct gccgtcgaaa acgcgttgaa gtacggtgcg
    12601 gcgcaaaccg aaacggggcc tgtattcgac aagctgggca acagtgcggc gattcaggaa
    12661 atgaaccgtg aggtaggggc tgcggtgaaa tgtgcctctc ccgtactttt gacgggcgag
    12721 gcgggttcgc cgtttgaaac ggtggcacgc tatttccata aaaacggtac gccgtgggtc
    12781 agcccggcaa gggtcgaata tctgatcgat atgccgatgg aactgttgca gaaggcggag
    12841 ggcggcgttt tgtatgtcgg cgacatcgcc cagtacagcc gcaacatcca agccggtatt
    12901 gcctttattg tcggaaaggc ggaacaccgc cgcgtcaggg tggtcgcatc gggcagcagg
    12961 gcggcaggtt cagacggcat tgcctgcgag gaaaagctgg cggaactgct gtcggagtcg
    13021 gtcgtccgca ttccgccgct gcgtatgcag catgaagata ttcccttcct gatacagggg
    13081 attgcctgca atgtggcgga aagtcaaaag attgcgcccg ccgcattcag tgaagaggca
    13141 cttgccgcat tgacccgtta cgactggccg ggaaatttcg accaactgca aagcgtcgtt
    13201 gcaacgctgt tgttggaggc ggacggacag gaaatcggcg cggcggcggt ttcttccctt
    13261 ttggggcaga atgtgcctgc cgagggagcg gaagatatgg tgggcgggtt taatttcaac
    13321 ctgcccctgc gcgaattgag ggaggaggtg gagcggcgtt atttcgagta ccacatcgcc
    13381 caagaaggtc agaatatgag caaagtggcg cagaaggtcg gtttggaacg cacgcacctt
    13441 taccgcaaac tcaaacagct cggcatcggc gtttcgcgcc gggcggggga aaaaaccgaa
    13501 gaataggccc ggacggtcgg ttttccggct gcgggctttt gttttcagac ggcatttggt
    13561 gcaaatgccg tctgaaatcg taaggggacg gattttatga cagaggacga acgtttcgcg
    13621 tggttgcaac tggcttttac gccctatatc ggtgcggaaa gtttcttgct gctgatgcgc
    13681 cgtttcggca gcgcgcaaaa tgccttgtcc gcaccggcgg aacaggtcgc cccggcggtg
    13741 cggcacaagc aggcggccga ggcttggcgc aatgcggaaa aacgcgcttt ggcgcggcag
    13801 gcggcagaag cggcgttgga atgggaaatg cgggacggat gccgcctgat gctgcttcag
    13861 gatgaagatt tccccgaaat gctgacgcag gggctgaccg cgccgccggt tttgtttttg
    13921 cgcggcaacg tgcggctgct gcacaagcct tccgccgcca tcgtcggcag ccgccatgcc
    13981 acgccgcagg cgatgcggat tgccaaagat ttcggcaagt cgttgggcgg gcaaaatatc
    14041 cccgttgtgt cgggtatggc ttccggtatc gatactgccg cccatcaggg cgcactggag
    14101 gcagaaggcg gcaccatcgc cgtgtggggg acgggtatag accgcattta cccgccgtcc
    14161 aataaaaacc ttgcctatga aatcgccgaa aaaggattga ttgtcagcga gtttcccatc
    14221 ggtacgcgcc cgtatgccgg caattttccg cgccgcaacc gcctgattgc cgccctgtcg
    14281 caagtaacgc tggtggttga agccgccttg gaatccggtt cgctgattac cgccggcctg
    14341 gcggcagaaa tggggcgcga agtcatggcg gtacccggtt cgatagacaa cccccacagc
    14401 aaaggctgcc acaaactgat taaagacggc gcaaaattgg tggaatgcct ggacgacatc
    14461 ctgaacgaat gcccggggct attgcaaaat acgggtgctt catcatattc tataaataag
    14521 gatacgcccg acacgggacg ccgcgccgtt cagacggcat acgccccgcc gcctgccgca
    14581 aaaatgccgt ctgaagcggc ggcaggcggc acagcccacg gcggcatact ggataaaatg
    14641 ggtttcgacc ccgtccatcc cgacgtgctt gccgagcagc ttgctatgcc tgccgcagat
    14701 ttgtatgccg cactgttgga attggaattg gacggcagcg ttgccgcaat gcccggcggc
    14761 agataccagc gtatccgaac ttgaacgcac tttatattaa ggaacacgaa tgaccgaagt
    14821 catcgcctac ctcatcgaac atttccaaga tttcgatacc tgcccgccgc ccgaagactt
    14881 gggtatgctg cttgaggaag cgggtttcga tacgatggaa atcggcaaca ccctgatgat
    14941 gatggaagta ttgctcaaca gctccgaatt ttccgccgaa cctgccgaca gcggcgcatt
    15001 gcgcgtgtac agcaaagaag aaaccgataa cctgccgcag gaagtgatgg ggctgatgca
    15061 gtatctgatt gaagaaaaag ccgtcagctg cgaacagcgg gaaatcatca tccacgcgct
    15121 gatgcacatt ccgggcgacg aaattaccgt agataccgcc aaagtgctga ccctgctgct
    15181 tttatgggca aacaagagcg agctgcccgt gttggtcggc gacgagctga tgagcgcgct
    15241 tttactcgac aacaaaccca cgatgaactg aagcggcttc agacggcccg cccgagtccg
    15301 tctgaaacgt cggcatcaaa accaccatcc agagaacgac aaatggcgaa aaacctatta
    15361 atcgtcgaat ccccgtccaa agccaaaacc ctgaaaaaat atttgggcgg cgattttgaa
    15421 atccttgcat cctacggaca cgtccgcgac ctcgtcccca aaagcggcgc ggtcgatccc
    15481 gacaacggct ttgcgatgaa ataccaactc atcagccgca acggcaaaca cgtcgatgcc
    15541 atcgtcgccg gtgccaaaga agctgaaaac atctacctcg ccaccgaccc ggatagggaa
    15601 ggcgaagcca tttcctggca tcttttggaa atcctcaaat ccaaacgcgg cttgaaaaac
    15661 atcaagccgc agcgtgtcgt gttccacgaa atcaccaaaa acgccgtgct cgatgccgtt
    15721 gcccatccgc gcgaaatcga aatggacttg gtcgatgcac aacaagcccg ccgtgctttg
    15781 gactatttgg tcggctttaa cctttcgcca ttgttatgga aaaaaatccg ccgaggtttg
    15841 agcgcggggc gtgtgcaaag ccctgctttg cgcctgattt gtgagcgcga aaacgaaatc
    15901 cgcgcgtttg aagcgcagga atattggacg gtgcatctcg acagccacaa aggccgcagc
    15961 aagttcaccg ccaaactcgc ccaatacaac ggcgcgaaac tcgaacaatt cgacctgtcg
    16021 aacgaagccg cgcaagccga cgtattgaaa gaactcgaag gcaaagaagc cgtcgttacc
    16081 gccattgaaa agaaaaagcg cagccgcaat cctgccgcgc cgttcaccac ttctaccatg
    16141 cagcaggatg ccgtgcgcaa actcggcttc accaccgacc gcaccatgcg taccgcccaa
    16201 cagctttatg aaggtataga cgtagggcag ggcgcgatcg gtctgattac ctatatgcgt
    16261 accgacagcg tgaatttggc ggatgaagcg ttaaccgaaa tccgccatta catcgaaaac
    16321 aaaatcggca aagaatatct gccgagtgcc gccaaacaat acaaaaccaa atccaaaaac
    16381 gcccaagaag cgcacgaagc gatccgcccg acttccgtgt accgcacgcc cgaaagcgtc
    16441 aaacccttcc tgagcgcaga ccagttcaaa ctctatcaaa tgatttggca gcgcaccgtc
    16501 gcctgtcaga tgacgcctgc caaattcgac caaaccaccg tcgatattac cgtcggcaaa
    16561 ggcgtattcc gcgtaaccgg acaagtgcaa accttcgcag gcttccttag cgtttacgaa
    16621 gaaagcaacg acgacgaaga gagtgaagac agcaaaaaac tacccgaaat gagcgaaggc
    16681 gacaaattgc ccgtggataa actttacggc gaacaacact ttaccactcc gccgccgcgc
    16741 tacaacgaag cgacgctggt taaagccctt gaagaatacg gcatcggccg cccttcgacc
    16801 tacgccagca tcatctccac gctcaaagac cgcgaatacg ttacccttga gcaaaaacgc
    16861 ttcatgccca ccgacacagg cgacatcgtc aacaaattcc tgaccgaaca cttcgcccaa
    16921 tacgtcgatt accacttcac cgccaaactc gaagaccagc ttgacgaaat tgccgacggc
    16981 aaacgccaat ggattccctt gatggacaaa ttctggaaac cgttcatcaa acaagtggaa
    17041 gaaaaagaag gcatcgaacg cgccaaattt accacgcagg aacttgatga aacctgcccg
    17101 aaatgcggcg aacacaaact gcaaatcaaa ttcggcaaaa tgggtcgttt tgttgcgtgt
    17161 gccggttatc ccgagtgcag ctacacgcgc aatgtcaacg aaaccgccga agaagctgcc
    17221 gaacgcatcg ccaaagccga agccgaacag gccgaactcg acggacgcga gtgcccgaaa
    17281 tgtggcggtc gcctagtgta caaatacagc cgcaccggca gcaaattcat cggctgcgcc
    17341 aactatccga aatgcaaaca cgtcgagccg ctggaaaaac cgaaagatac cggcgtccag
    17401 tgtccgcaat gcaaaaaagg caacctcgtc gagcgcaaat cccgctacgg caaactgttt
    17461 tacagttgca gcacctatcc cgactgcaac tacgccactt ggaacccgcc cgttgccgaa
    17521 gaatgcccga actgccattg gccggtcttg accatcaaaa ccactaaacg ctggggtgtg
    17581 gaaaaagtct gcccgcaaaa agaatgcggc tggaaagaac agatcgaacc gcctgcaccg
    17641 aaagagtgag atcaggttgg tttgaaagag aaaaggtcgt ctgaaaaatt ttcagacgac
    17701 ctttgctttt ctgtgattgg tttatttgaa tccgcgtgtt gttttaaagt ccgataaaat
    17761 ccggttcatt tcaggcacaa acaaggcgat gtaatcgtaa gatagaccgc gactggcact
    17821 gggatgggga aagcagacga cttcgcaatc ttcaaacgat tggaatttga cattgaaacg
    17881 tgtaccgtca aattcttttt gcaccgtctc cagcggtttg gtctgcttac cgaccaactg
    17941 ctcgaagcgt ggcagtacat tttggttgtt cagaaaatcc gccaacctgc tgcccatgaa
    18001 gaggatgact ttcggacgca gtttttcgat gtggtagaga aaattatcga tgtgctcggg
    18061 ttgtgtgaac ttgtcgggat tgtcgatagt gttgccctgt gtagcagccc agttggtttg
    18121 aaccagggat ttttcaaatg caccgcccaa tccattttcg tctaaggggt gtccccacat
    18181 ttcaaaccaa ttttttatcg tattgtcgta acgccacttt tttgcctgct ctccgaaata
    18241 gagggatttg tttgcaaatg tatggtcgat tttgttttca gggagtttgt attcacctgc
    18301 tacataagca gcctcatcgg ctttactcca accccattca tagccacaaa tcattaagcc
    18361 atgtttgtcg ttgtagcctt tgaacaggct gttgctcaaa ttcaaatcct tcatcatgaa
    18421 ctcttccttt taaaatttaa gagcgattga cttcaatgtt tttagatggg gtggaaaaat
    18481 ccttgtgtag gcaacataaa ttcaataaat ttcttgataa ttcgaaacct actaatagcg
    18541 cacctataaa agctttttca ttacgttcag catgacggtc acgtcgttca tattttttac
    18601 gcttgctgtt ccctgttatt acagctaagc caagtgatat ggcgagaatt gcccaaacaa
    18661 tagtacttaa ataacaattt tccccatact atcaataagg aaagaaaaaa ccttttagta
    18721 ttaggtcgat agattataat ccatgcccat gaaaatgcaa taagagttat acataagata
    18781 caagctgaca ggatttcttt atttttaatt aaataactta gagcgatgag gatgacaggt
    18841 gtcaagaaaa taatagttac atcccgatag ctataaaaga aaactgccct attttgaatg
    18901 tggagatgtg cacagaatcc aatatagcta aggataatag ttaatataat aaaaaaagac
    18961 caccaagggt gaagagatag gaattccatg ttttcccttt ttttgtaaaa aggaaaaaat
    19021 cttatctaat agttaattgc ctaaccagcc agaagaagtt taaaatctat cccaataatt
    19081 caaccatcta tacagaaagt tcagcttatg gaaacccacg aaaaaatccg cctgatgcgc
    19141 gaattgaata aatggtccca ggaggatatg gcggaaaagc tggcgatgtc ggcaggaggg
    19201 tatgccaaaa tcgaacgggg cgaaacgcag ttgaatatcc cgcgtttgga gcagttggct
    19261 cagattttca aaatcgatat gtgggacttg ctcaaatcgg gcggtggtgg gatggtgttt
    19321 cagattaatg aaggtgatag tggtggcgat attgcgttgt atgcgtcggg tgatgtttcg
    19381 atgaaaatag aatttttaaa aatggagttg aaacactgca aagaaatgtt ggaacaaaaa
    19441 gacaaagaaa tcgagctgct ccgcaagctg accgaaaccg tttaaacaga tatgccgtct
    19501 gaaaaaagtt ttcagacggc atattctttg acaggtcttg tataataccg tttgaactta
    19561 caggtttttg attatggcgg caggcaaaca taccaaacac agcaaccggg tacgcattat
    19621 cggcgggcaa tgccggggca ggaaattgag tttcacatcc gccgacggac tgcgtccgac
    19681 acccgacagc gtgcgtgaaa agctgtttaa ctggctggga caggatttga cgggtaaaac
    19741 ggttttggat ctcttcggag gcagcggcgc actcggtata gaagccgctt cgcgcaacgc
    19801 caaacgcgtg ctgatttcgg ataacaaccg ccaaaccgtg cagaccttgc agaaaaacag
    19861 tcgcgaactg ggtttggggc aggtgcaaat cgtcttttca gacggcatcg catatttgaa
    19921 gaccgtatcc gaacagtttg atgttgtctt tctcgacccg ccgtttgcat ggcaggactg
    19981 gcaaatcctg ttcgatgcct tgaagccgtg cctgaacccc cgggcattcg tctatctcga
    20041 ggcgggtacg ctgccgaata ttcccgattg gctgacggaa tatagagaag ggaaatcggg
    20101 gcagagtaca ttcgaattaa gggttttcca agtggctgaa taatatgcgc tttgataatc
    20161 atttccgagt tgtaaacatt cgtttgcaac cgtccggttc aaaaaaacct tgtgctataa
    20221 tccgcgcccg cccggttttg ataatttagt ggaaaaggaa aagaaatgtc gctttttatt
    20281 accgacgagt gcatcaactg cgacgtatgc gaacccgaat gccccaatga tgccatttcc
    20341 caaggcgagg aaatttacga aatcaacccc aacctctgca cgcagtgcgt cggacactac
    20401 gatgagccgc agtgccagca ggtttgcccg gtggactgca tcctgattga cgaagaacat
    20461 cccgaaaccc atgacgagtt gatggcgaaa tacgaaaaga ttatccagtt taaataaatt
    20521 ctttttaaaa catcaaatta tgtctgtttt gaaataaaat caaaaaaaaa cttgacggaa
    20581 aagcaagccg ctaataaact aacgttctct tttggaggga ttcccgagcg gtcaaagggg
    20641 gcagactgta aatctgttgc gaaagcttcg aaggttcgaa tccttctccc tccaccaaaa
    20701 ttcttacttg gggcagtagc gagtaatgcg ggtgtagctc aatggtagag cagaagcctt
    20761 ccaagcttac ggtgagggtt cgattccctt cacccgctcc aaacaattag gcccatgtag
    20821 ctcaggggta gagcactccc ttggtaaggg agaggtcggc agttcaaatc tgcccatggg
    20881 caccatccct cgattattca tttctttaag gcttagatat ataggatatt g
//
LOCUS       DAXHSF010000018        20707 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.018, whole genome shotgun sequence.
ACCESSION   DAXHSF010000018 DAXHSF010000000
VERSION     DAXHSF010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20707
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.018"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(64..3669)
                     /gene="putA"
                     /locus_tag="ACFM8S_000534"
     CDS             complement(64..3669)
                     /gene="putA"
                     /locus_tag="ACFM8S_000534"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HFC6313653.1"
                     /translation="MFHFAFPAQTALRQAITDAYRRNEIEAVQDMLQRAQMSDEERNA
                     ASELARRLVTQVRAGRTKAGGVDALMHEFSLSSEEGIALMCLAEALLRIPDNATRDRL
                     IADKISDGNWKSHLNNSPSLFVNAAAWGLLITGKLTATNDKQMSSALSRLISKGGAPL
                     IRQGVNYAMRLLGKQFVTGQTIEEALQNGKEREKMGYRFSFDMLGEAAYTQADADRYY
                     RDYVEAIHAIGKDAAGQGVYEGNGISVKLSAIHPRYSRAQHGRVMGELLPRLKELFLL
                     GKKYDIGINIDAEEANRLELSLDLMEALVSDPDLAGYKGIGFVVQAYQKRCPFVIDYL
                     IDLARRNNQKLMIRLVKGAYWDSEIKWAQVDGLNGYPTYTRKVHTDISYLACARKLLS
                     AQDAVFPQFATHNAYTLGAIYQMGKGKDFEHQCLHGMGETLYDQVVGPQNLGRRVRVY
                     APVGTHETLLAYLVRRLLENGANSSFVNQIVDENISIDTLIRSPFDTIAEQGIHLHNA
                     LPLPRDLYGKCRLNSQGVDLSNENVLQQLQEQMNKAAAQDFHAASIVNGKARDVGEAQ
                     PIKNPADHDDIVGTVSFADAALAQEAVGAAVAAFPEWSATPAAERAACLRRFADLLEQ
                     HTPALMMLAVREAGKTLNNAIAEVREAVDFCRYYANEAEHTLPKDAEAVGAIVAISPW
                     NFPLAIFTGEVVSALAAGNTVIAKPAEQTSLIAGYAVSLMHQAGIPTSALQLVLGAGD
                     VGSALTGDTRIGGVIFTGSTEVARLINKALSKRDDNPVLIAETGGQNAMIVDSTALPE
                     QVCLDVLNSAFDSAGQRCSALRILCVQEDVADKMVNIIKGAMDELVVGKPTQLTTDIG
                     PVIDAEAQQNLLVHINKMKGVAKSYHEVKTAADVDSEKSTFVRPILFELNNLNELQRE
                     VFGPVLHVVRYRADELDSVIDQINSKGYALTHGVHSRIEGTVRHIRSRIEAGNVYVNR
                     NIVGAVVGVQPFGGHGLSGTGPKAGGSFYLQKLTRAGEWVAPTLSQIGQADEAALKRL
                     EALVHKLPFNAEEKKAAAAALGHARIRTLRRAETVLTGPTGERNSISWHAPKRVWIHG
                     GSTVQAFAALTELAASGIQAVVEPDSPLASYTADLEGLLLVNGKPETAGISHVAALSP
                     LDSARKQELAAHDGALIRILPSENGLDILQVFEEISCSVNTTAAGGNASLMAVAD"
     gene            3661..3828
                     /locus_tag="ACFM8S_000535"
     CDS             3661..3828
                     /locus_tag="ACFM8S_000535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313654.1"
                     /translation="MKHFLDSPKFFGGQAASVRPEYGNIVINPQMKYKASNAGRVGAS
                     VSLKMKRVKHK"
     gene            complement(3918..5444)
                     /gene="putP"
                     /locus_tag="ACFM8S_000536"
     CDS             complement(3918..5444)
                     /gene="putP"
                     /locus_tag="ACFM8S_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFC6313655.1"
                     /translation="MNPMYITFAIYLVAVLLIGLAAYFSTRNFDDYILGGRSLGPFVT
                     AMSAGASDMSGWLLMGLPGAIYLSGLNEAWIAIGLLVGAYFNWLLVAGRLRVHTEYAN
                     NALTLPDYFFHRFGAGGHLMKVVSALIILFFFTIYCASGIVAGATLFQSLFEGMTYNQ
                     AMWLGAGATIAYTFLGGFLAVSWTDTLQASLMIFALILTPVMVYLGLGGAEQMSAAIQ
                     SVAAGTGKEYGSLFAGTTVIGIISTAAWGLGYFGQPHILARFMAAESAKSLVSARRIG
                     MTWMALCLAGAVAVGYFGIAYFGANPDKVSSMSGNHERIFIALSTLLFNPWIAGIILS
                     AILAAVMSTLSCQLLVCSSAITEDFYKGFLRKNAQQSELVWVGRLMVLAIAVISILIA
                     SDPNSKVLGLVSYAWAGFGAAFGPIVILSVLWKRITAYGALSGMVAGALTVVVWAEWI
                     KKPAQAAGESGLLTMYEIVPGFIVCLIIAVLVSLFNKEPSREIQKRFEKADADYRAAR
                     "
     gene            5927..6247
                     /locus_tag="ACFM8S_000537"
     CDS             5927..6247
                     /locus_tag="ACFM8S_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HFC6313656.1"
                     /translation="MFGKQLFEEVGSKISETIANSPAKDVEKNIKAMLGGAFNRMDLV
                     TREEFDIQQQVLIKTRTKLAALEARLAKLEAAQNPERAALEAAEAAAEEAVAEIRQQT
                     EAGE"
     gene            6261..7757
                     /locus_tag="ACFM8S_000538"
     CDS             6261..7757
                     /locus_tag="ACFM8S_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFC6313657.1"
                     /translation="MSLALVYSRALSGMNAPLVEVEAHLANGLPHFNIVGLPDTEVKE
                     SRDRVRAAIIQSGFEFPAKKITVNLAPADLPKESGRFDLPIAIGILAASGQVAPEKLA
                     EYEFAGELALSGLLRPVRGALAMAWQGMQAKRAFVLPEENAGQAAVMRGITVYGARSL
                     GEVAAHLNGIEPLVQTDCQVPQMPSENTKLLDLVDVKGQHTARLALEIAAAGGHSLLM
                     MGPPGTGKSMLSQRLPGILPPLTEDELVEVWALRSLLPNHQQQLDSNRPFRSPHHSAS
                     AAAMVGGGSDPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLENGEIHISRAARQA
                     VYPAKFQLVAAMNPCPCGYLGHPVKPCRCTPESVARYRSKISGPLLDRIDLTIEVPSL
                     SAAELMQQEAGESSASVLERVIAARDKQYARQGKVNAALSVSELDTSARIQKEAQEAL
                     GGLLEKLSLSARSFHRIMRVARTLADLAGDEEVGRSHVMKAIGFRRAL"
     gene            7863..8726
                     /gene="ftsN"
                     /locus_tag="ACFM8S_000539"
     CDS             7863..8726
                     /gene="ftsN"
                     /locus_tag="ACFM8S_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFC6313658.1"
                     /translation="MNKFSQSGKGLSGFFFGLILATVIIAGILFYLNQSGQNAFKIPA
                     SSKQPAETEILKPKNQPKEDIQPEPADQNALSEPDAATEAEQSDAEKAADKQPVADKA
                     DEVEEKAGEPEREEPDGQAVRKKALTEEREQTVREKAQKKDAETVKKQAVKPSKETEK
                     KASKEEKKAEKEKVAPKPTPEQILNSGSIEKARSAAAKEVQKMKTSDKAEATHYLQMG
                     AYADRRSAEGQRAKLAILGIYSKVVGYQAGHKTLYRVQSGNMSADAVKKMQDELKKHE
                     VASLIRSIESK"
     gene            8729..9373
                     /locus_tag="ACFM8S_000540"
     CDS             8729..9373
                     /locus_tag="ACFM8S_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFC6313659.1"
                     /translation="MKLKHLLPLLLSAVLSAQAYALTEGEDYLVLDKPIPQKQSGKIE
                     VLEFFGYFCVHCHHFDPLLLKLGKALPSDAYLRTEHVVWQPEMLGLARMAAAVKLSGL
                     KYQANPAVFKAVYEQKIRLENRSVAEKWALSQKGFDGKKLMRAYDSPAAAAAASKMQQ
                     LTEQYRIDSTPTVVVGGKYRVIFNNGFDGGVHTIKELVAKVREERKRQTPAVQK"
     gene            9406..10227
                     /locus_tag="ACFM8S_000541"
     CDS             9406..10227
                     /locus_tag="ACFM8S_000541"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFC6313660.1"
                     /translation="MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLIDFHSNHK
                     VFEITIQLGAVLAVVFEYRQRFSNVLHGVGKDRKANRFVLNLAIAFIPAAVMGLLFGK
                     QIKEYLFNPLSVAVMLVLGGFFILWVEKRQSRAEPKIVDVDALRPIDALMIGVAQVFA
                     LVPGTSRSGSTIMGGMLWGIERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVG
                     LILIGFVAAFVSGLVAVKALLRFVSKKNYIPFAYYRIVFGIAIIILWLSGWISWE"
     gene            complement(10518..10593)
                     /locus_tag="ACFM8S_000542"
     tRNA            complement(10518..10593)
                     /locus_tag="ACFM8S_000542"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10558..10560),aa:Ala,seq:ggc)
     gene            complement(10601..10677)
                     /locus_tag="ACFM8S_000543"
     tRNA            complement(10601..10677)
                     /locus_tag="ACFM8S_000543"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10641..10643),aa:Met,seq:cat)
     gene            complement(10809..12026)
                     /locus_tag="ACFM8S_000544"
     CDS             complement(10809..12026)
                     /locus_tag="ACFM8S_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6313661.1"
                     /translation="MTAPVPTCNMSGNPINLHKLTLIMPLPLPSPEARQSSLKLQTLI
                     AEKIGKHGNWIPFSRFMELVLYAPQYGYYTGGSHKIGNTGDFITAPTLTSLFAQTLAR
                     QLQELLSQTAGNIYEFGAGTGQLAADLLGSISDGISRYYIIEISPELAARQKNLIQAR
                     APEASQKVVHLTALPEAFDGIIIGNEVLDAMPVEIVRKNEGGSFEHVGVCLDNDRFTY
                     SARPLHDLQLSALASLYFPQTDYPYTSELHPQQYAFIRTLASRLEHGCMIFIDYGFDA
                     AQYYHPQRNQGTLIGHYRHHVIHNPFDFIGLADLTAHVNFTDIAQAGTDAGLDLIGYL
                     PQSHFLLNLGITELLAQTGKTNSAAYIREAAAVQKLIDQHEMGELFKVIAFGKNIGID
                     WAGFRFGDICHKL"
     gene            12305..12760
                     /gene="mraZ"
                     /locus_tag="ACFM8S_000545"
     CDS             12305..12760
                     /gene="mraZ"
                     /locus_tag="ACFM8S_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFC6313662.1"
                     /translation="MFGGAHELSIDSKGRLAVPAKFRDILSRLYTPAVVVTLESKHKL
                     LMYPVAEWEKVAAQLLNLKVADNPVLRRFQNLLLHNAEILEWDSAGRVLLPAGLRKRV
                     DFDREVVLVGRANRLELWGREQWEAEMVQALDDDPDELAFQLSQTDLQL"
     gene            12757..13743
                     /gene="rsmH"
                     /locus_tag="ACFM8S_000546"
     CDS             12757..13743
                     /gene="rsmH"
                     /locus_tag="ACFM8S_000546"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFC6313663.1"
                     /translation="MSGAESYRHITVLLNEAVDALAVREDGVYVDGTFGRGGHSRLIL
                     SRLGDAGRLIVFDKDPQAIAVAEELARSDKRVGVVHGGFASFQTALDGLGIGKVDGAL
                     FDLGISSPQIDDGSRGFSFRFDAPLDMRMDTTRGMSAAEWIAVASEQDLHEVIKNYGE
                     ERFSRQIARAIVAQRAESPIDTTRKLAQIVAQNVRTRERGQDPATRTFQAVRIFINRE
                     LEEVGAVLPQVMCRLKEGGRLAVIAFHSLEDRIVKQFVKKYSQHAPLPRWAAVREADL
                     PEPPLKIVGRALKPGEAEIAANPRARSAVLRVAERTAGPIPEQSQRKTSEWQ"
     gene            13740..14003
                     /gene="ftsL"
                     /locus_tag="ACFM8S_000547"
     CDS             13740..14003
                     /gene="ftsL"
                     /locus_tag="ACFM8S_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFC6313664.1"
                     /translation="MNKLNFLLLLAVCVSAFSVVMQQNQYRLNFTALDKAKKQEIALE
                     QDYAQMRLQQARLANHEAIRAAAEKQNLHPPVSGNTFMVEHQR"
     gene            14064..15809
                     /locus_tag="ACFM8S_000548"
     CDS             14064..15809
                     /locus_tag="ACFM8S_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFC6313665.1"
                     /translation="MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAIAVLFAGLIA
                     RGLYLQTVTYNFLKEQGDNRIVRTQTLPATRGTVSDRNGAVLALSAPTESLFAVPKEM
                     KEMPSAAQLERLSELVDVPVDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFV
                     FEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLHGEDGAEVVLRDRQGNIV
                     DSLDSPRNKAPKNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLDARTGEIL
                     ALANTPAYDPNRPGWADSEQRRNRAVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNT
                     QPYKIGPSPVRDTHVYPSLDVRGIMQKSSNVGTSKLSARFGAEEMYDFYHELGIGVRM
                     HSGFPGETAGLLRNWRRWRPIEQATMSFGYGLQLSLLQLARAYTALTHDGVLLPVSFE
                     KQAVAPQGKRIFKESTAREVRNLMVSVTEPGGTGTAGAVDGFDVGAKTGTARKFVNGR
                     YADNKHIATFIGFAPAKNPRVIVAVTIDEPTAHGYYGGVVAGPPFKKIMGGSLNILGI
                     SPTKPLTAAAVKTPS"
     gene            15834..17312
                     /locus_tag="ACFM8S_000549"
     CDS             15834..17312
                     /locus_tag="ACFM8S_000549"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFC6313666.1"
                     /translation="MFSKLTPLAETGIPTLSCANAAGRLLHSDSRQIKQGDIFVACPG
                     EYADGRSYIPAAVANGAAFVFWDDDGKFAWNPEWKVPNQGIKDLKHRAGILAAQVYGN
                     VSDGLKFWGVTGTNGKTSITQWLAQAADLLGEKTAIVGTVGNGFWGALEETTHTTPAP
                     VDVQTLLYRFRQQGATVAAMEVSSHGLDQSRVNGVSFRSAIFTNLTRDHLDYHGTMEA
                     YGAIKSRLFYWHGLKHAVINVDDEYGAELAGRLKKDCPDLAVYSYGFSEHADIRITDF
                     TASSDGIAAVFQTPWGEGKCRTRLLGRFNAQNLAACIALLCANGYPLDNVLDVLAKIR
                     PASGRMDCIMNSGKPLVVVDYAHTPDALEKALATLQEIKPQGAALWCVFGCGGNRDRG
                     KRLLMGAAAVQGADKVVVTSDNPRLENPHDIINDILPAVPAPECVEADRAAAIRYAVE
                     QAAANDIILIAGKGHENYQDVQGVKHRFSDLEIVGQALLTRK"
     gene            17335..18981
                     /locus_tag="ACFM8S_000550"
     CDS             17335..18981
                     /locus_tag="ACFM8S_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFC6313667.1"
                     /translation="MKQSARIKNMNQTLKNTLGICALLAFCFGAAIASGYHLEYEYGY
                     RYSAVGALASVVFLLLLARGFPRVSSVVLLIYVGTTALYLPVGWLYGAPSYQIVGSIL
                     ESNPAEAREFVGNLPGSLYFVQALFFIFGLTVWKYCVSGGVFADVKNYKRRSKIWLTI
                     LLTLILSCAVMDKIASDKDLREPDAGLLLNIFDLYYDLASAPAQYAAKRAHILEAAKK
                     ASTWHIRHVAPKYKNYVVVIGESARSDYMNVYGFPLPDTPFLSQTKGLLINGYQSTAH
                     ATNLSLPQTLGLPGEPNNNIVSLAKQAGFRTAWLSNQGMLGHFANEISTYALRSDYPW
                     FTQRGDYGKSAGLSDRLLLPAFKRVLIGNAGTKPRLIVMHLMGSHSDFCTRLDKDARR
                     FQYQTEKISCYVSTIAQTDKFLEDTVKILNENKESWSLVYFSDHGLMHVGKGGERTLT
                     HGAWKRQSYGVPLVKISSDDTRREMIKVRRSAFNFLRGFGSWTGIETDELPDDGYDFW
                     GNVPDVQGEGNNLAFIDGLPDDPAPWYAGKGKSTKNTSKK"
     gene            19107..20465
                     /gene="murF"
                     /locus_tag="ACFM8S_000551"
     CDS             19107..20465
                     /gene="murF"
                     /locus_tag="ACFM8S_000551"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFC6313668.1"
                     /translation="MKPLDLNFICQALKLPMPSENKSVSRIVTDSRDIQEGDVFFALA
                     GERFDAHDFVEDVLAAGAAAVVVSREDCVAMDGALKVDDTLTALQMVAKAWRENVDPF
                     VFGITGSGGKTTVKEMLASVLRRRFGDNAVLATAGNFNNHIGLPLTLLKLNEKHRYAV
                     IEMGMNHFGELAVLTQIAKPDAALVNNALRAHVGCGFDGVGDIAKAKSEIYQGLCSDG
                     MALIPQEDANMAVFKTATLNLNTRTFGIDSGDVHAENIVLKPLSCEFDLVCGDERAAV
                     VLPVPGRHNVHNAAAAVALSLAAGLSLNDVAEGLKGFSNIKGRLNVKAGIKGATLIDD
                     TYNANPDSMKAAIDVLARMPAPRIFVMGDMGELGEDEAAAMHAEVGAYARDQGIEAAY
                     FVGDNSVEAAEKFGADGLWFTAKDPLIQVLRHDLPERATVLVKGSRFMQMEEVVEALE
                     DK"
     gene            20468..20644
                     /locus_tag="ACFM8S_000552"
     CDS             20468..20644
                     /locus_tag="ACFM8S_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313669.1"
                     /translation="MKSRRFFKALLLIAALVGAFYAGMQTQAYLYEDLCLDLGGGKNP
                     GSYPICVIEKVPAR"
BASE COUNT         4832 a         5042 c         5975 g         4858 t
ORIGIN      
        1 tgccgtctga aaggttttca gacggcattg gttcacggcc cgcgcccggg gatttcggta
       61 aaatcagtcg gcgaccgcca tcaggctggc gttgccgccg gcggctgtgg tgttgacgct
      121 gcaagagatt tcttcaaaca cttgcaggat gtcgagtccg ttttccgaag ggaggatgcg
      181 gatgagtgcg ccgtcgtggg cggcaagttc ctgtttgcgt gcgctgtcca aaggcgacag
      241 ggcggcaacg tggctgatgc cggcggtttc gggtttgccg ttgaccagca gcagaccttc
      301 caagtcggcg gtgtaggaag ccaaggggct gtcgggttcg accactgcct gtatgccgga
      361 ggcggcaagt tcggtcagtg cggcaaaggc ttgaaccgtg ctgccgccgt gtatccaaac
      421 gcgtttgggc gcgtgccatg agatgctgtt gcgctcgccg gtcggtccgg taaggacggt
      481 ttcggcacgg cgcagggtgc ggatgcgggc gtgtcccaaa gcggccgctg cggctttttt
      541 ctcttcggcg ttgaacggta gtttgtgaac cagtgcttcg aggcgtttga gtgcggcttc
      601 gtccgcctgt ccgatttggc tcagggtcgg ggcaacccat tcgccggcgc gggtcagttt
      661 ttgcaggtag aacgaaccgc ctgctttggg gcctgtgccg gacagaccgt gtccgccgaa
      721 gggctgtacg ccgacgactg cgccgacgat gttgcggttg acgtaaacgt tgccggcttc
      781 gatgcggctg cggatgtggc gtaccgtgcc ttcgatgcgg ctgtgtacgc cgtgggtcag
      841 ggcgtagcct ttgctgttga tttggtcgat gacgctgtcg agttcgtcgg cgcggtagcg
      901 gacgacgtgc aggacgggac cgaagacttc gcgttgcagt tcgttgaggt tgttcaattc
      961 aaacaggatg gggcggacga acgtggattt ttcggaatcg acatcggcag cggttttgac
     1021 ttcgtggtaa gacttggcaa cgcctttcat tttgttgatg tggaccaaca ggttttgttg
     1081 ggcttcagca tcgatgacag ggccgatgtc tgtggtcagt tgggtcggtt tgccgacaac
     1141 caattcgtcc attgcgcctt tgatgatgtt gaccatttta tcggcaacat cttcttgaac
     1201 gcataagata cgcagagcgg aacaacgttg gcctgcgctg tcgaaggcag agttcaaaac
     1261 gtcgaggcag acttgttccg gcagggcggt agagtccaca atcatcgcgt tttgaccacc
     1321 ggtctcggca atcaggacag ggttgtcgtc gcgtttggac agtgctttgt taatcaggcg
     1381 tgctacttcg gtagaaccgg tgaagatgac gccgccgatg cgggtgtcgc cggtcagggc
     1441 agagccgaca tcacctgcgc cgagtacgag ttgcagcgca gaagttggaa taccggcttg
     1501 atgcatcagg gaaacggcgt aaccggcaat caggctggtt tgttcggcag gtttggcgat
     1561 gacggtgtta cctgccgcca atgcggacac gacttcgccg gtaaagatgg cgagcgggaa
     1621 gttccacggg ctgatggcga caatcgcgcc gacggcttcg gcatctttgg gcagggtgtg
     1681 ttcggcttcg tttgcgtagt agcggcagaa atcgacggct tcgcgcactt cggcaatggc
     1741 gttgttcagc gttttgcctg cttcgcgcac ggcaagcatc atcagcgcgg gggtgtgctg
     1801 ttcgagcagg tcggcgaaac ggcgcaggca ggcggcgcgt tcggcggcag gtgtcgcact
     1861 ccattcgggg aacgcggcaa cggctgcgcc aaccgcttct tgggcaagcg cggcatcggc
     1921 aaagctgact gtgccgacga tgtcgtcgtg gtcggcaggg tttttaatcg gttgcgcttc
     1981 gccgacatcg cgggctttgc cgttgacgat ggatgcggcg tggaagtctt gcgcggcggc
     2041 tttgttcatc tgttcttgaa gctgctgcaa tacgttttcg ttgctcaagt ccacgccttg
     2101 cgagttcaga cggcatttgc cgtacaaatc gcgcggcagc ggcagggcgt tgtgcaggtg
     2161 gatgccttgt tcggcgatgg tgtcgaacgg gctgcggatg agcgtgtcga tgctgatgtt
     2221 ttcatcgacg atttggttga cgaaagacga gttcgcgccg ttttccaaca ggcggcgcac
     2281 caagtaggcg agcagggttt cgtgtgtgcc gaccggggcg tacacgcgca cgcggcggcc
     2341 taagttttgc gggccgacga cttggtcgta cagggtttcg cccatgccgt gcagacattg
     2401 gtgttcaaag tctttgcctt tgcccatttg gtagattgcg cccaaggtgt aggcgttgtg
     2461 ggtggcgaat tgcggaaata ccgcgtcttg cgcggaaagc agtttgcgcg cgcaggcgag
     2521 gtaggagatg tcggtgtgga ctttgcgggt gtaggtcgga tagccgttca agccgtccac
     2581 ttgcgcccat ttgatttcgc tgtcccaata cgcgcctttg acgaggcgga tcattagttt
     2641 ttggttgttg cggcgggcaa ggtcgatcag gtagtcgata acgaacggac aacgtttttg
     2701 gtaggcttgg acaacgaaac cgatgccttt gtagcctgcc aaatcgggat cggaaacgag
     2761 tgcctccatc aaatccaaag acagctccag acggttggct tcttcggcat cgatgttgat
     2821 accgatatcg tattttttac ccaaaaggaa cagctctttc aggcgcggca acagttcgcc
     2881 catcacacgg ccgtgttggg cgcgcgagta gcgcggatgt atggcggaga gtttgacgga
     2941 aatgccgttg ccttcgtaaa cgccttgtcc tgccgcatct ttgccgatgg cgtggatggc
     3001 ttcgacatag tcgcggtagt agcggtcggc atcggcttgg gtgtaggcgg cttcgcccaa
     3061 catatcgaag gagaagcggt agcccatttt ttcgcgttct ttgccgtttt gcagggcttc
     3121 ttcaatggtc tgtccggtta cgaactgttt gcccaacagg cgcatggcgt aatttacgcc
     3181 ttggcggatg agcggtgcgc cgcctttgct gatcaggcgg ctgagtgcgg aactcatttg
     3241 tttgtcgttt gtggcggtca gtttgccggt aatcagcagg ccccaggcgg cggcgttgac
     3301 gaagagggaa gggctgttgt tcaaatggct tttccagttg ccgtctgaaa tcttgtcggc
     3361 aatcaggcgg tcgcgcgtgg cgttgtcggg gatacgcagc agggcttctg ccagacacat
     3421 cagcgcgatg ccttcttcgc tggagagtga aaactcgtgc atcagcgcat ctacgccgcc
     3481 ggctttggtg cggccggcgc ggacttgggt aaccaaacgg cgggcaagct cggaggcggc
     3541 gttgcgctct tcgtcgctca tctgtgcacg ttgcaacata tcctgtacgg cttcgatttc
     3601 attacggcgg taggcatcgg ttatcgcttg gcgcagggca gtttgtgccg gaaatgcaaa
     3661 atgaaacatt ttttggattc tccaaagttt ttcggggggc aggcggcatc ggtgcggcct
     3721 gaatacggta atatcgtaat aaatccgcag atgaaataca aggcttcaaa tgcgggcagg
     3781 gtaggtgctt ccgtttcttt gaaaatgaaa cgggtaaaac acaaataagg cctgtatgca
     3841 ggcaaggttt atttgtgttt gacccggaaa cgggttcaga cggcacgaac cgggatgccg
     3901 tgccgtctga aaggggttta tcgggcggcg cggtaatctg cgtcggcttt ttcaaagcgt
     3961 ttttggattt cgcgcgaagg ttctttgttg aacagggaaa ccaacacggc aatgattaag
     4021 caaacaataa agcccgggac gatttcgtac atcgtcaaca agccgctttc gcctgctgct
     4081 tgagccggtt tttttatcca ttccgcccat acgactacgg ttaaggcacc tgcaaccata
     4141 cccgacaacg cgccgtaggc agtgatgcgt ttccacaata cggacagaat cacaatcggg
     4201 ccgaacgccg caccgaaacc tgcccacgca taagacacca gtcccaatac tttgctgttc
     4261 ggatcggaag caatcaggat ggaaatcacg gcaatcgcca agaccatcag gcggccgacc
     4321 cataccagtt ccgactgttg cgcgttttta cgcaaaaagc ctttgtagaa gtcttcggta
     4381 atcgcgctgg agcaaaccaa aagctggcag gacagggtgg acatcaccgc cgccaaaatc
     4441 gcgctcaaaa tgatgccggc gatccaaggg ttgaacagca gggtggaaag cgcgatgaag
     4501 atgcgttcgt ggttgccgct catagaagaa actttgtcgg gatttgcacc gaaatacgca
     4561 atgccgaaat aaccgaccgc caccgcgccc gcaaggcaca acgccatcca agtcatgccg
     4621 atgcggcgtg cggataccag cgatttcgcg ctttcggccg ccataaagcg cgccaaaatg
     4681 tgcggctgtc cgaaatagcc caagccccat gcggcggtgg aaatgatgcc gatgacggtc
     4741 gtaccggcaa acaggctgcc gtattctttg cccgtgcctg cggcgacgct ttgaatcgcg
     4801 gcagacatct gttccgcgcc gcccaagccc agatagacca ttaccggcgt taaaatcagc
     4861 gcgaaaatca tcaaagaagc ctgcaacgta tccgtccagc tgaccgccaa gaagccgccc
     4921 aagaaggtat aggcgatggt cgcgcccgcg cccagccaca ttgcctgatt gtaagtcata
     4981 ccttcaaaca ggctttggaa cagggttgcg cccgccacaa tgcccgaggc gcaataaatc
     5041 gtgaagaaga acaggataat cagtgcggaa accactttca tcaagtgtcc gcccgcgccg
     5101 aagcggtgga agaaataatc cggcagcgtc agcgcattgt tggcgtattc ggtatgcacg
     5161 cgcaggcggc ccgccaccaa gagccagttg aaatacgcgc cgaccaagag gccgatggca
     5221 atccaagcct cattcaaacc gctcaggtaa atcgcgcccg gcagacccat cagcagccag
     5281 ccggacatat cggacgcgcc tgccgacatc gcggtaacaa acgggcctag gctgcgcccg
     5341 cccaaaatat aatcgtcgaa attgcgcgta gaaaaatagg cggcaagccc gatgagaagg
     5401 actgcaacca gatagattgc aaaagtaatg tacatgggat tcatgtgcta ttcctcgtct
     5461 aaaacttcag aattacaggc tttgaaattg caagcaactt gcgcctgaaa tgtttttcta
     5521 ataaaagtac aacggaaaat ccggataccc gaaaggggga ttcggataaa ttatcttcaa
     5581 tcacaataag atatgtaata aaactatatg aaattgtaaa taatccgttt cagcataacc
     5641 caatttctgt tgtttgcaaa gcacttaatg gcttaaaaag ccgagtttga aacgatgcgc
     5701 gtcggaaaaa tcatttaaaa cagtatattg ttttgtaatg tcttgtaatc tggcgttgcg
     5761 cggaatatga aatccgtttt caggcggcag gtgttttgag gtgtaattta gcaaccgcaa
     5821 aggaggcgcg gtatgttttg ccgattatcc gccgcccgtt ttcagacggc atttttcctt
     5881 atacaatagc cgattgaatt tgatatgttc aggaaggata cagattatgt tcggcaagca
     5941 gctttttgaa gaagtcggtt cgaaaatcag cgaaaccatc gccaacagcc ctgccaaaga
     6001 tgtggaaaaa aatattaagg cgatgctggg cggcgcgttc aaccgtatgg atctggttac
     6061 gcgcgaagaa ttcgacatcc agcagcaggt tttaatcaaa actcgtacca aactggcggc
     6121 tttggaagcg cgtttggcaa aactcgaagc cgcgcaaaat cccgaacggg cagcattgga
     6181 agcggctgaa gccgctgccg aagaagccgt cgccgaaatc aggcagcaaa ccgaagccgg
     6241 cgaataaggc cgtctgaaat atgtcgcttg ccttggttta cagccgcgcc ttgagcggta
     6301 tgaatgcgcc gttggtcgaa gtggaagccc accttgccaa cggcctgccg catttcaaca
     6361 tcgtcgggct gcccgatacg gaagtaaagg aaagtcgcga ccgcgtccgt gccgccatta
     6421 ttcaaagcgg ttttgaattc cccgccaaaa aaattaccgt caacctcgcc cccgccgacc
     6481 tgcccaaaga gtcggggcgt ttcgatttgc cgattgcaat tggcatcctt gccgcatcgg
     6541 ggcaggttgc acccgaaaaa ctggcggaat acgagtttgc gggggaattg gcactgtcgg
     6601 ggctgttgcg ccccgtgcgt ggcgcgttgg cgatggcgtg gcagggtatg caggcaaaac
     6661 gtgcatttgt tttgcctgaa gaaaacgcag gacaagccgc cgtgatgcgc ggcattaccg
     6721 tttacggcgc gcgctctttg ggcgaagtcg ccgcccattt gaacggtatc gaacctttgg
     6781 tgcaaaccga ctgtcaagtt cctcagatgc cgtctgaaaa caccaaattg ctcgacctcg
     6841 tcgatgtcaa gggtcagcac actgcccgcc ttgctttgga aatcgctgcc gcaggcggac
     6901 acagcctttt gatgatgggt ccgccgggaa cgggcaagtc tatgctctcc caacggctgc
     6961 ccggcatcct gccgccgctg accgaagacg aattggtaga agtttgggca ttgcgttcgc
     7021 tcctgcccaa ccaccagcaa caactcgaca gcaaccgtcc tttccgcagt ccgcatcaca
     7081 gcgccagcgc ggcggctatg gtcggcggcg gttcggatcc gcgtccgggc gaaatttcat
     7141 tggcgcatca cggcgtttta tttttggatg aattgcccga gttcgaccgc aaagttttgg
     7201 aagttttgcg cgaaccgttg gaaaacggcg aaatccacat ttcccgagcg gcgcgccaag
     7261 ccgtctatcc cgccaaattc caacttgtcg ccgccatgaa cccctgcccg tgcggctatc
     7321 tcgggcatcc cgtcaaaccc tgccgctgca cgcccgaaag cgtcgcgcgt taccgcagca
     7381 aaatttccgg cccgctgctc gaccgcatcg atttgaccat cgaagtcccc agcctgtccg
     7441 ccgccgaact gatgcagcag gaagcgggtg aaagcagcgc gtccgttttg gaacgcgtta
     7501 tcgccgcccg cgacaaacaa tacgcacggc aaggcaaagt gaatgccgcc ttgagtgtca
     7561 gtgaactcga cacatccgcc cgcattcaaa aagaagcgca ggaagcattg ggcggcctgc
     7621 tggaaaaact ctccctttcc gcccgcagct tccaccgcat tatgcgcgtg gcgcgtacat
     7681 tggcggattt ggcgggcgac gaagaagtcg gcagaagcca cgtcatgaaa gccataggtt
     7741 tccgtcgcgc tttataggaa cgggaatgga agcaggtttt gcccaaatat ggcgatattg
     7801 ttagaatatc cgcccgtaag caaacggcgt taatgccgtc tgaaacacat taaggtatgt
     7861 ttatgaacaa attttcccaa tccggaaaag gtctgtccgg ttttttcttc ggtttgatac
     7921 tggcgacggt cattattgcc ggtattttgt tttatctgaa ccagagcggt caaaatgcgt
     7981 tcaaaatccc ggcttcgtcg aagcagcctg cagaaacgga aatcctgaaa ccgaaaaacc
     8041 agcctaagga agacatccaa cctgaaccgg ccgatcaaaa cgccttgtcc gaaccggatg
     8101 ctgcgacaga ggcagagcag tcggatgcgg aaaaagctgc cgacaagcag cccgttgccg
     8161 ataaagccga cgaggttgaa gaaaaggcgg gcgagccgga acgggaagag ccggacggac
     8221 aggcagtgcg taagaaagcg ctgacggaag agcgtgaaca aaccgtcagg gaaaaagcgc
     8281 agaagaaaga tgccgaaacg gttaaaaaac aagcggtaaa accgtctaaa gaaacagaga
     8341 aaaaagcttc aaaagaagag aaaaaggcgg aaaaggaaaa agttgcaccc aaaccgaccc
     8401 cggaacaaat cctcaacagc ggcagcatcg aaaaagcgcg cagtgccgcc gccaaagaag
     8461 tgcagaaaat gaaaacgtcc gacaaggcgg aagcaacgca ttatctgcaa atgggcgcgt
     8521 atgccgaccg ccggagcgcg gaaggacagc gtgccaaact ggcaatcttg ggcatatatt
     8581 ccaaagtggt cggttatcag gcgggacata aaacgcttta ccgcgtgcaa agcggcaata
     8641 tgtctgccga tgcggtgaaa aaaatgcagg acgagttgaa aaaacatgaa gtcgccagcc
     8701 tgatccgttc tatcgaaagc aaataattat gaagctcaaa catctgttgc cgctgctgct
     8761 gtcggcagtg ttgtccgcgc aggcatatgc cctgacggaa ggggaagact atcttgtgtt
     8821 ggataaaccc attcctcaaa aacagtcggg caaaattgag gttttggaat ttttcggcta
     8881 tttctgcgta cattgccatc atttcgatcc tttgttattg aaattgggca aggcattgcc
     8941 gtctgatgcc tatttaagga cggagcacgt ggtctggcag cctgaaatgc tcggtctggc
     9001 aagaatggct gctgcggtca agctgtcagg tttgaaatat caggcaaacc ctgccgtgtt
     9061 taaagcagtt tacgaacaaa aaatccgctt ggaaaacagg tcggttgccg aaaaatgggc
     9121 tttgtctcaa aaaggctttg acggcaaaaa actgatgcgc gcctacgact ctcctgcggc
     9181 agcggctgct gcatcaaaaa tgcagcaatt gacggaacag taccgcatcg acagtacgcc
     9241 gaccgttgtc gtcggcggaa aataccgcgt tatcttcaat aatggctttg acggcggtgt
     9301 tcatacgatt aaagaattgg ttgccaaagt cagggaagaa cgcaagcgtc agacccctgc
     9361 tgtacagaaa tagccgaact cccgtatccg aaagaagcgc aagcaatgga ttttctgatt
     9421 gtcctgaaag ccctgatgat gggcttggta gaaggtttta ccgaattttt accgatttcc
     9481 agcaccggac atttgattgt gttcggcaat ctgattgatt ttcacagcaa tcacaaggtt
     9541 tttgaaatta ccatccagct cggtgcggtt ttggcggtag tgtttgaata ccggcagcgt
     9601 ttcagcaatg tgttgcatgg cgtgggaaaa gaccggaaag ccaaccgttt cgtccttaat
     9661 cttgccattg cttttatacc tgccgccgtg atggggctgt tgttcggcaa acaaatcaaa
     9721 gagtatctgt ttaacccctt gagtgttgca gtcatgctgg ttttgggcgg tttttttatt
     9781 ttgtgggtgg agaaacgcca aagccgggca gagcctaaaa ttgtcgatgt tgatgcattg
     9841 cgtccgattg atgcgttgat gatcggcgtt gcccaagtgt ttgcactggt tccgggtacg
     9901 tcccgttcgg gcagtacgat tatgggcggg atgctttggg gaatcgagcg gaaaacggca
     9961 acggagtttt catttttctt ggccgttccg atgatggttg cagcaacggc ttatgatgtc
    10021 ctgaagcatt accggttttt caccctgcat gatgtcggtt tgattctgat tggctttgtt
    10081 gccgcttttg tttccggttt ggtggcggta aaagcgttgc tgaggtttgt ttccaagaaa
    10141 aattatattc cttttgccta ttaccgcatt gtttttggca ttgccatcat tatattgtgg
    10201 ctgtcaggct ggataagttg ggaatgaaac cataaacccg acctgaagac attattcggg
    10261 tcgggtttgt ttggcgggct gatatagtga attaacaaaa atcaggacaa ggcgacgaag
    10321 ccgcagacag tacagatagt acggcaaggc gagccaacgc tgtaccggtt taaatttaat
    10381 tcactataaa atcaggacag gcggggcgat aggtttaaag tcgattgcct gttttgaagg
    10441 cagtggttta ttctttattt gccggcaatc aggcaataaa aaagcacata cctttttacg
    10501 gtctgtgctt ttttatctgg tggaggtaag cgggatcgaa ccgctgacct cttgcatgcc
    10561 atgcaagcgc tctaccaact gagctatacc cccgaaaatt tggtggcgaa tcagggactc
    10621 gaaccccgga cacaaggatt atgattcctc tgctctaacc gactgagcta attcgccgtt
    10681 tcgtgaagac gctattatat gtttttctgt ttttttgaca agccgtattt tttaattttg
    10741 aattagttga ctgtttttaa atgttaaaaa gtttatgccg tctgaagcgg attcaggcgg
    10801 catgagggtt agagtttgtg gcagatgtcg ccgaagcgga atcctgccca gtcgatgccg
    10861 atattttttc cgaatgcgat gactttaaac agttcgccca tttcatgctg gtcaatcagt
    10921 ttctgaacgg cagcagcttc acggatatag gctgccgaat tcgttttccc cgtctgtgcc
    10981 aatagctcgg taatgcccag gttcaataag aaatgggatt ggggaaggta acctatcaaa
    11041 tctaatccgg catccgtccc ggcttgggca atgtcggtaa agttgacatg cgcggtcagg
    11101 tcggccaatc cgatgaagtc aaaaggattg tggataacgt gatgtcggta gtgtccgatc
    11161 agagtacctt gattgcgttg agggtggtaa tactgcgctg catcaaaacc gtagtcgatg
    11221 aatatcatgc agccgtgttc gagtctggag gcaagggtgc ggataaaggc atattgttgc
    11281 ggatgtagtt cgctggtgta gggataatct gtttgaggaa aatagaggga agccaaggca
    11341 gatagctgta agtcgtgcag cggtcgtgcc gaataggtaa aacggtcatt atctaggcaa
    11401 acgccgacat gctcgaatga gccgccttca tttttacgga cgatttcgac aggcatggca
    11461 tcgagtactt cgttgccgat gatgatgccg tcaaacgctt cgggaagtgc ggtcaagtgg
    11521 acaacttttt gagatgcttc cggtgcgcgg gcttgaatca ggtttttctg acgtgctgcc
    11581 agctccggcg atatttcaat aatatagtaa cggctgatgc cgtccgaaat gctgcccaac
    11641 aaatcggcgg caagctgtcc ggttcccgcg ccgaattcat agatattgcc cgccgtttga
    11701 gatagaagtt cttgaagttg gcgtgccagt gtctgtgcaa acagagaggt gagggtcggt
    11761 gcggtaataa aatccccggt attgccgatt ttatggctgc cgccggtgta gtagccgtat
    11821 tgcggagcgt ataaaaccaa ttccataaaa cgtgaaaatg gaatccagtt gccgtgttta
    11881 ccgatttttt cggcaatgag ggtttgcagt ttgagcgagg attgccgtgc ttcgggagag
    11941 gggaggggga ggggcatgat aagtgttagc ttgtgtaaat ttattggatt tcccgacata
    12001 ttacacgttg gtacgggtgc tgtcatggct ttatcttaat actatatatt gtgtttatat
    12061 tattaaatta atcatatata gttgtttatt ggttcggtca ctctgtaccg cacccgccgt
    12121 gccgttgtcg taatttttta tcttattgtt tttaaaagga ataaaaattt cagatatgtt
    12181 aatgagtttt catgccctga tttgaccgag tgtttaaaat ttcttatagt gtcgattggt
    12241 ggggaattgt ggggcaaagt gtctctttta cccttgtgat tttgatttcg gcttgggaca
    12301 tgtcatgttc ggcggcgcac acgaattaag catcgacagt aaggggcggt tggctgttcc
    12361 tgccaaattc cgtgacattc tgtcgcgcct ctatacgcct gccgtagtgg taacgctcga
    12421 gtcgaaacac aagctgttga tgtaccctgt tgcggagtgg gaaaaggttg cggcgcaact
    12481 tttaaactta aaagtggcgg ataaccctgt tttgcggcgg tttcaaaatc ttttgctgca
    12541 taacgcggaa attttggaat gggacagcgc cggccgggtg ctgcttcctg ccggactgag
    12601 gaaaagggtg gatttcgacc gtgaagtcgt tttggtcggt cgtgccaacc gtttggagct
    12661 ttggggtcgc gagcagtggg aggctgagat ggttcaggct ttggatgacg atcctgacga
    12721 acttgccttc cagttgagtc agacggattt gcaattgtga gtggagcaga aagttaccgg
    12781 catatcacgg tcttgctgaa tgaggcggtg gatgcgcttg ccgtgcgcga agacggtgtc
    12841 tatgtggacg gtacgttcgg caggggaggg cattcccggc tgattttgtc gcgtttgggc
    12901 gatgcggggc ggttgattgt tttcgacaaa gacccgcagg cgattgctgt ggcagaagag
    12961 ctggcgcgtt cggacaaacg ggtcggtgtc gtgcatggcg gttttgcttc gtttcagacg
    13021 gcattggacg gtttgggtat cggcaaggtg gacggtgcgc tgtttgattt ggggatttcg
    13081 tccccgcaaa tcgatgacgg cagccgcggt ttcagcttcc gtttcgatgc ccctttggat
    13141 atgcgtatgg atacgacgcg cggtatgtct gccgcagagt ggatagcggt tgcgtcggaa
    13201 caggatttgc acgaggtaat caagaattat ggtgaagagc ggtttagccg ccagattgcg
    13261 cgcgccattg ttgcgcaacg ggcggaaagt ccaatcgata caacccgcaa gctggcgcag
    13321 atcgtggcac aaaacgtccg tactcgcgag cgggggcagg atcctgcgac gcgcaccttc
    13381 caggcggtcc gcatctttat taaccgcgag cttgaagaag taggggcagt attgccgcag
    13441 gtcatgtgtc gtctgaaaga gggcggacgt ttggcggtca ttgctttcca ttcgttggaa
    13501 gaccgcattg tgaagcagtt tgtcaaaaaa tattcgcaac acgcgcccct gccgcgctgg
    13561 gcggcggtca gggaagcgga tttgcccgag ccgcccctga aaatcgtggg cagggcatta
    13621 aagccgggtg aggcggaaat tgccgccaat ccgagggcga gaagtgcggt tttgcgtgtg
    13681 gcggagcgga ctgccggtcc gataccggaa caatcacaga gaaaaacgtc tgaatggcaa
    13741 tgaacaaatt gaatttcctt ctgctgcttg cggtgtgcgt ttccgctttt tccgttgtga
    13801 tgcagcaaaa ccagtacagg ctcaacttca cagctttgga caaggcgaaa aaacaggaaa
    13861 tcgccttgga gcaggattat gcgcaaatga ggctgcaaca ggcgcgtttg gcgaaccacg
    13921 aagcgatcag ggcggcggca gaaaaacaaa acctccatcc gccggtttcg ggcaatacct
    13981 ttatggtgga gcatcaaaga tagaagcagc ctgtgtgccg gaatcggatt cctgcgtcag
    14041 gataataata acgagaagta aaaatgttga ttaagagcga atataagcct cggatgctgc
    14101 ccaaagaaga gcaggtcaaa aagccgatga ccagtaacgg acggattagc ttcgtcctga
    14161 tggcaatagc ggtcttgttt gccggtctga ttgcccgcgg actgtatctg cagacggtaa
    14221 cgtataactt tttgaaagaa cagggcgaca accggattgt gcggactcaa acattgccgg
    14281 ctacacgcgg tacggtttcg gaccggaacg gtgcggtttt ggcgttgagc gcgccgacgg
    14341 agtccctgtt tgccgtgcct aaagagatga aggaaatgcc gtctgccgca caattggaac
    14401 gcctgtccga gcttgtcgat gtgccggttg atgttttgag gaacaagctc gaacagaaag
    14461 gcaagtcgtt tatctggatt aagcggcagc tcgatcccaa ggttgccgaa gaggtcaaag
    14521 ccttgggttt ggaaaacttt gtatttgaaa aagaattaaa acgccattac ccgatgggca
    14581 acctgtttgc acacgtcatc ggatttaccg atattgacgg caaaggtcag gaaggtttgg
    14641 aactttcgct tgaagacagc ctgcatggcg aagacggcgc ggaagtcgtt ttgcgggacc
    14701 ggcagggcaa tattgtggac agcttggact ccccgcgcaa taaagccccg aaaaacggca
    14761 aagacatcat cctttccctc gatcagagga ttcagacctt ggcctatgaa gagttgaaca
    14821 aggcggtcga ataccatcag gcaaaagccg gaacggtggt ggttttggat gcccgcacgg
    14881 gggaaatcct cgccttggcc aatacgcccg cctacgatcc caacaggccc ggctgggcag
    14941 acagcgaaca gcggcgcaac cgtgccgtaa ccgatatgat cgaacccggt tcggcaatca
    15001 aaccgtttgt gattgcgaag gcattggatg cgggcaaaac cgatttgaac gaacggctga
    15061 atacgcagcc ttataaaatc ggaccgtctc ccgtgcgcga tacccatgtt tacccctctt
    15121 tggatgtgcg cggcatcatg cagaaatcgt ccaacgtcgg cacaagcaaa ctgtctgcgc
    15181 gtttcggtgc cgaagaaatg tatgacttct atcatgagtt gggcatcggt gtgcgtatgc
    15241 actcgggctt tccgggcgaa actgcaggtt tgttgagaaa ttggcgcagg tggcggccca
    15301 tcgaacaggc gacgatgtct ttcggttacg gcctgcaatt gagcctgctg caattggcgc
    15361 gcgcctatac cgcactgacg cacgacggcg ttttgctgcc ggtcagcttt gaaaaacagg
    15421 cggttgcgcc gcaaggcaaa cgcatattca aagaatcgac cgcgcgcgag gtacgcaatc
    15481 tgatggtttc cgtgaccgag ccgggcggca ccggtacggc gggtgcggtg gacggtttcg
    15541 atgtcggcgc gaaaaccggc acggcgcgca agttcgtcaa cgggcgttat gccgacaaca
    15601 aacacatcgc tacctttatc ggttttgccc ccgccaaaaa cccccgtgtg attgtggcgg
    15661 taaccattga cgaaccgact gcccacggtt attacggcgg cgtagtggca gggccgccct
    15721 tcaaaaaaat tatgggcggc agcctgaaca tcttgggcat ttccccgacc aagccactga
    15781 ccgccgcagc cgtcaaaaca ccgtcttaat ccgagtatca acgagattgt tttatgttca
    15841 gcaagttaac ccctttggct gaaaccggca tcccgaccct gtcgtgtgca aacgcggcag
    15901 ggcgtttgtt gcattcagac agccgccaaa tcaaacaagg cgatattttc gttgcctgtc
    15961 cgggcgaata tgccgacgga cgcagttata tccccgccgc cgttgccaac ggcgcggctt
    16021 ttgttttttg ggacgacgac ggcaaattcg cgtggaatcc cgaatggaaa gtccccaatc
    16081 aaggcatcaa agatttgaaa caccgtgccg gcatattggc ggcgcaagtt tacggcaacg
    16141 tttcagacgg tctcaaattc tggggcgtaa ccggaaccaa cggcaaaacc tccatcacgc
    16201 aatggctggc gcaagctgcc gatttgttgg gcgaaaaaac cgccattgtc ggcacggtcg
    16261 gcaacggctt ttggggtgca ttggaagaaa ccacgcatac cacacccgcc cccgtcgatg
    16321 tccaaaccct gctctaccgt ttccgtcaac aaggcgcaac agtcgccgcg atggaagtct
    16381 ccagccacgg gcttgaccag tcgcgcgtca acggcgtgtc attccgcagc gcaatcttta
    16441 ccaacctcac ccgcgaccac ctcgactacc acggcacgat ggaagcctac ggtgccatca
    16501 agtcgcgcct gttttactgg cacggcttga aacacgccgt tatcaatgta gatgacgaat
    16561 acggcgcgga actcgcaggt cgtctgaaaa aagattgtcc cgatttggcc gtttatagct
    16621 acggcttcag cgaacacgcc gacatccgca ttaccgactt taccgcctct tcagacggca
    16681 tagcagccgt attccaaacc ccgtggggcg aagggaaatg ccgcacgcgc ctgctcggac
    16741 ggttcaacgc gcaaaacctc gccgcctgca tcgccttgct gtgcgccaac ggctatccgc
    16801 ttgataatgt attggatgtg ctggcaaaaa tccgtcccgc ttcagggcgc atggactgca
    16861 tcatgaacag cggcaaacct ttggtcgttg tcgattatgc ccacacgccc gacgcattgg
    16921 aaaaagcact cgccaccttg caggaaatca aaccgcaggg tgcggctttg tggtgcgtat
    16981 tcggttgcgg cggtaaccgc gatcgcggca aacgcctgct gatgggcgcg gcagccgtac
    17041 agggtgcgga taaagtcgtc gtcaccagcg acaacccgcg tttggaaaat ccgcacgaca
    17101 tcatcaacga catcctgcct gccgttcccg cgcccgaatg cgtcgaagcc gaccgtgccg
    17161 ccgccatccg ttatgcggtt gaacaagccg ccgcaaacga catcatcctg attgccggca
    17221 aagggcatga aaactatcag gatgtacaag gcgtgaagca ccgtttttcc gatcttgaaa
    17281 tcgtcggaca ggctttgtta actcgtaaat aatgggatat tcggacggca tcgtatgaaa
    17341 caatccgccc gaataaaaaa tatgaatcag acattaaaaa atacattggg catttgcgcg
    17401 cttttagcct tttgttttgg cgcggccatc gcatcaggtt atcacttgga atatgaatac
    17461 ggctaccgtt attctgccgt gggtgctttg gcttcggttg tatttttatt attattggca
    17521 cgcggtttcc cgcgcgtttc ttcagttgtt ttactgattt acgtcggcac aaccgcccta
    17581 tatttgccgg tcggctggct gtatggtgcg ccgtcttatc agatagtcgg ttcgatattg
    17641 gaaagcaatc ctgccgaggc gcgtgaattt gtcggcaatc ttcccgggtc gctttatttt
    17701 gtgcaggcat tatttttcat ttttggcttg acagtttgga aatattgtgt atcggggggg
    17761 gtatttgctg acgtaaaaaa ctataaacgc cgcagcaaaa tatggctgac tatattattg
    17821 actttgattt tgtcctgcgc ggtgatggat aaaatcgcca gcgataaaga tttgcgagaa
    17881 cctgatgccg gcctgttgtt gaatattttc gacctgtatt acgatttggc ttccgcgccg
    17941 gcacaatatg ccgccaagcg cgcccacatt ttggaagcag caaaaaaagc gtcaacatgg
    18001 catatccgtc atgttgcgcc caagtataaa aattatgttg tggttatcgg tgagagcgcg
    18061 cgttcggatt atatgaatgt ttacggtttc ccattgcccg atacgccttt tttgagtcag
    18121 accaaagggc tgttgataaa cggttaccaa tcgaccgccc acgcgacgaa tctttcgctg
    18181 ccgcagactt tggggctgcc gggagaaccg aacaataaca tcgtcagctt ggcgaagcag
    18241 gcgggttttc ggacggcgtg gctgtctaat caaggaatgt tggggcattt tgccaacgaa
    18301 atttccacct atgccctacg cagcgattat ccgtggttta cccaaagggg tgattatggc
    18361 aaaagcgcgg ggttgagcga ccgccttttg ttgccggcgt tcaaacgggt tttgatagga
    18421 aatgcaggca cgaagcctcg gctgattgtg atgcacctga tgggttcgca cagtgatttt
    18481 tgcacacgtt tggataagga tgcgcggcgg tttcagtatc aaactgaaaa aatatcctgc
    18541 tatgtttcca ccatcgcgca aaccgataaa tttttagaag atacagttaa gatattgaat
    18601 gaaaataaag aaagctggtc tttggtttac ttttccgacc acggtttgat gcatgtcggt
    18661 aaaggcggcg agcgaacgtt gacacatggt gcgtggaagc gtcaaagcta cggcgtgccg
    18721 ctggttaaaa tttcgtccga tgacacgcgg cgcgaaatga ttaaagtgag gcgcagcgcg
    18781 tttaattttt tacgcggatt cggcagttgg acgggtatcg aaaccgacga gttgcccgat
    18841 gacggctatg atttttgggg gaatgttccc gatgtgcagg gcgaaggcaa taaccttgcc
    18901 tttatcgacg gactgcccga cgaccccgcg ccgtggtatg cgggaaaagg caaatcgact
    18961 aaaaatacgt ctaaaaaatg atacgtacag aaaaaatgcc gaatgagaat gggaaaataa
    19021 tctgtgtttt accacagcaa aacaggcgat aaaaaaaatc agccgctacc gatgtgttcg
    19081 ccgcccgaat attaacgaaa gtaaatatga aaccactgga cctaaatttc atctgccaag
    19141 ccctcaagct tccgatgccg tctgaaaaca aatccgtgtc gcgcatcgta accgacagcc
    19201 gcgatattca ggaaggcgat gtgtttttcg cattggcggg cgagcggttt gatgcgcatg
    19261 attttgttga agacgtattg gctgcaggtg cggcggcggt tgtggtttcg cgcgaagatt
    19321 gcgttgcaat ggatggcgcg ttgaaagtcg atgacacgct taccgcgttg caaatggtgg
    19381 cgaaggcgtg gcgtgaaaac gtggatccgt ttgtgttcgg cattaccggt tcgggcggca
    19441 agacgacggt gaaggaaatg ctggcttcgg tattgcgccg ccgtttcggc gataatgccg
    19501 ttttggcgac ggcaggcaac ttcaacaacc acatcggatt gccgttgact ttgttgaaat
    19561 taaacgaaaa acaccgctat gccgtgattg aaatgggtat gaatcatttt ggcgaactgg
    19621 cggttttgac gcaaatcgcc aaacctgatg ccgccttggt taacaacgcc ctgcgtgccc
    19681 atgtcggctg cggtttcgac ggagtgggcg atattgccaa agcgaaaagc gagatttatc
    19741 aaggcttatg ttcagacggc atggcgctga ttcctcaaga agatgccaat atggctgtct
    19801 tcaaaacggc aacgcttaat ttgaatacgc gcactttcgg catcgatagc ggcgatgtcc
    19861 acgcggaaaa tatcgtgctg aaaccgttgt cgtgcgaatt tgatttggtg tgcggcgacg
    19921 aacgcgccgc cgtggtgctg cctgttcccg gccgccacaa tgtccacaac gccgccgccg
    19981 ccgtcgcgct gtctttggct gcaggtttga gtttgaacga tgtggcggaa ggtttgaaag
    20041 gcttcagcaa tatcaaaggc cgtctgaacg tcaaagccgg catcaagggc gcgaccctga
    20101 ttgacgatac ttataatgcg aatcccgaca gcatgaaagc cgcgattgac gtgttggcgc
    20161 gtatgcctgc gccgcgtatt ttcgtgatgg gcgatatggg cgaactgggt gaggacgaag
    20221 ccgccgccat gcacgccgaa gtcggcgcgt atgctcgcga ccaaggcatc gaagcggctt
    20281 attttgtcgg cgacaacagc gtcgaagcgg cggaaaaatt tggcgcggac ggtttgtggt
    20341 tcaccgccaa agacccgttg attcaagtgt tgcgccacga tttgcccgaa cgcgccaccg
    20401 tgttggtgaa aggttcgcgc tttatgcaga tggaagaagt ggtcgaggca ttggaggata
    20461 agtgaaaatg aaaagtcgac gtttttttaa agccttattg ttgattgccg cgctggtcgg
    20521 cgcgttttat gccggaatgc agacgcaggc gtatctttat gaagatttat gtttagactt
    20581 gggcggcggc aaaaatccgg ggagttaccc gatttgcgtg attgagaaag tccctgcacg
    20641 ttaatctgca aaagccgtcc gaaaccttgc cgggcggcaa gccaacctca aacgggcgca
    20701 ggcccga
//
LOCUS       DAXHSF010000019        20211 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.019, whole genome shotgun sequence.
ACCESSION   DAXHSF010000019 DAXHSF010000000
VERSION     DAXHSF010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20211
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.019"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(527..1669)
                     /locus_tag="ACFM8S_000553"
     CDS             complement(527..1669)
                     /locus_tag="ACFM8S_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFC6313670.1"
                     /translation="MKKSVLAVLAALSLAACGGSEKNAVQPQADAASAANAEAAATDT
                     LNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFL
                     PRQIEAGAYQKVNKDLIPNYKNIDPELLKMLETADPGNQYAVPYFSGVNTIAITAKGK
                     ELLGGKLPENGWDLLFKPEYTHKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDL
                     KAAAEVLKSIRPDVKRFSPSIIDELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEV
                     LNPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIAVTFAPASKPAREKM
                     PAELVNTRSIFPNEQDMKDGFVMPQMSTDAKKLSVSLWQKIKVGTN"
     gene            1973..2236
                     /gene="rpsT"
                     /locus_tag="ACFM8S_000554"
     CDS             1973..2236
                     /gene="rpsT"
                     /locus_tag="ACFM8S_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFC6313671.1"
                     /translation="MANSAQARKRARQSVKQRAHNASLRTAFRTAVKKVLKAVEAGDK
                     AAAQAVYQESVKVIDRIADKGVFHKNKAARHKSRLSAKVKALA"
     gene            2396..3544
                     /locus_tag="ACFM8S_000555"
     CDS             2396..3544
                     /locus_tag="ACFM8S_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFC6313672.1"
                     /translation="MPTMGAEMNTRNMRYILLTGLLPMASAFGETALQCAALTDNVTR
                     LACYDRIFAAQLPSSAGQEGQESKAVLNLTETVRSSLDKGEAVIVVEKGGDALPADSA
                     GETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPLWYNNSPNYAPGSPTRGTTVQ
                     EKFGQQKRAETKLQVSFKSKIAEDLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTD
                     YKPEIFLTQPVKADLPFGGRLRMLGAGFVHQSNGQSRPESRSWNRIYAMAGMEWGKLT
                     VIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYRLNDRQNVYSVLRYNPKTGYGAI
                     EAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGIGLMFNDLDGI"
     gene            3687..4388
                     /locus_tag="ACFM8S_000556"
     CDS             3687..4388
                     /locus_tag="ACFM8S_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HFC6313673.1"
                     /translation="MTEPAAEGGKAAKALKKYLITGILVWLPIAVTVWVVSYIVSASD
                     QLVNLLPKQWRPQYVLGFNIPGLGVIVAIAVLFVTGLFAANVLGRQILAAWDSLLGRI
                     PVVKSIYSSVKKVSESLLSDSSRSFKTPVLVPFPQSGIWTIAFVSGQVSNAVKAALPK
                     DGDYLSVYVPTTPNPTGGYYIMVKKSDVRELDMSVDEALKYVISLGMVIPDDLPVKTL
                     AGPMPPEKADLPEQQ"
     gene            4446..6254
                     /gene="aspS"
                     /locus_tag="ACFM8S_000557"
     CDS             4446..6254
                     /gene="aspS"
                     /locus_tag="ACFM8S_000557"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFC6313674.1"
                     /translation="MRTNYCGLISEQYLDQTVTVKGWVHRRRDHGGVIFIDLRDREGI
                     VQVVIDPDTPEAFAAADSSRNEYVLSITGRVRNRPEGTTNDKMISGKIEILAKEIEVL
                     NAAATPPFQIDDENISENVRLTNRVIDLRRPVMQRNLRLRYQVAMGVRRYLDAQGFID
                     IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDLETSFLNEDEIMDITEGMAKQVFKDALGVDLGDFPRMPYSEA
                     MFYYGSDKPDMRINLKFTELTDLMKTEEFKVFRGAADMKGGRVVALRVPNGAKFSRKE
                     IDEYTKFVGIYGAKGLAYIKVNDVGNLSNGEDSGLQSPIVKFLSENALKEIIARTGAQ
                     NGDIIFFGADKTKVVNEAIGALRIKVGLEHGKDNGYFTDEWKPLWVVDFPMFEYDEEA
                     DRYVAVHHPFTAPKEGHEDLMVSDPANCLARAYDMVLNGWEIGGGSIRIHRADVQEKV
                     FAALKISPEEQQEKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMTGAESIRDVIAFPKT
                     QRAQCLLTNAPNSVDDKQLRELSLRLRQKAAETKEA"
     gene            6405..6689
                     /locus_tag="ACFM8S_000558"
     CDS             6405..6689
                     /locus_tag="ACFM8S_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFC6313675.1"
                     /translation="MKKLLLAAVVSLSAATAFADDSAERQIYGDPHFEQNRTKAVKML
                     EQRGYQVHDVDADGHWGKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDR"
     gene            6856..8748
                     /gene="speA"
                     /locus_tag="ACFM8S_000559"
     CDS             6856..8748
                     /gene="speA"
                     /locus_tag="ACFM8S_000559"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFC6313676.1"
                     /translation="MPILTIREVCNINHWGIGYYDVDDSGEIIVRPNPAQHNQTVSLQ
                     KLTEAVQQKHQARLPVLFCFPQILEHRLRDINRAFQTAREECGYKGGYCLVYPIKVNQ
                     HRRVIESLMSSGQPHGLEAGSKAELMAVLAHAGTRQTLIVCNGYKDREYIRFALMGEK
                     LGHQVYLVIEKLSEIQMVLEEAEKLGVKPRLGVRARLASQGSGKWQSSGGEKSKFGLS
                     ASQVLQLVDILKQKNRLDCLQLLHFHLGSQLGNIRDVATGVHESARFYVELHKLGVNI
                     RCFDVGGGLGVDYEGNRTQSDCSVNYSLNEYAATVVWGISQACLEHGLPHPTIITESG
                     RGITAHHAVLVANVIGVERYKPRRLDAPSPEAPRVLHSMWETWTDISASREKRSLRSW
                     IHEGQFDLADVHNQYNVGLLSLAQRAWAEQLYLNICHEVGELFNEKHRSHRTIIDELQ
                     ERFADKLYVNFSLFQSLPDAWGIDQLFPVCPITGLNEPIARRAVLLDITCDSDGTIDH
                     YIDGDGIAGTMPMPDYPEEEPPLLGFFMVGAYQEILGNMHNLFGDTATADVVVGEDGQ
                     FTVIDYDEGNTVADMLEYVYQDPKELMKRYREQIEHSDLPASQAMSFLKELEAGLNGY
                     TYLEDE"
     gene            8847..9770
                     /gene="speB"
                     /locus_tag="ACFM8S_000560"
     CDS             8847..9770
                     /gene="speB"
                     /locus_tag="ACFM8S_000560"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFC6313677.1"
                     /translation="MQYSTLAGQTDNSLVSNNFGFLRLPLNFMPYESHADWVITGVPY
                     DMAVSGRSGARFGPEAIRRASVNLAWEHRRFPWTFDVRERLNIIDCGDLVFSFGDSRD
                     FVEKMEAHAGKLLSSGKRCLSLGGDHFITLPLLRAHARYFGKLALIHFDAHTDTYDNG
                     SEYDHGTMFYTAPKEGLIDPSRSVQIGIRTEHSKKLPFTVLSAPKVNEDSVEETVRKI
                     KETVGNMPVYLTFDIDCLDPSFAPGTGTPVCGGLSSDRALKILRGLTDLDIVGMDVVE
                     VAPSYDQSDITALAGATIALEMLYLQGAKKD"
     gene            9968..11434
                     /locus_tag="ACFM8S_000561"
     CDS             9968..11434
                     /locus_tag="ACFM8S_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HFC6313678.1"
                     /translation="MMKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFVGV
                     IAAIIGKAMPLGALSIIAVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMIS
                     RGLLKTGLGMRIGYLFIAVFGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQ
                     SIAGSYGSNPAKGTEGKMGKYLALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSS
                     FRLSWGAWAWAMAVPGVIAFFVMPLILYFLYPPEIKETPNAVQFAKDRLREMGKMSAD
                     EIIMAVIFGILLLLWADVPALITGNHAFSINATATAFIGLSLLLLSGVLTWDDVLKEK
                     SAWDTIIWFGALIMMAAFLNKLGLIKWFSGVLAESVGGLGVSGTAAGVILVLAYMYAH
                     YMFASTTAHITAMFGAFFAAAVSLNAPAMPTALMMAAASNIMMTLTHYATGTSPVIFG
                     SGYTTMGEWWKAGFIMSVVNFLIFFVIGSIWWKVLGYW"
     gene            complement(11641..12717)
                     /locus_tag="ACFM8S_000562"
     CDS             complement(11641..12717)
                     /locus_tag="ACFM8S_000562"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFC6313679.1"
                     /translation="MICRKPIGHTPYFDMNKTICRTAALLISGFSYANTVIPDVSPVA
                     QGQHVFINIPQQRLFLYTDGKLTKVYPVAVGRAMTQTNLGEHKIGAKAYNPVWYIPKS
                     IQKERGDGVKTIAAGPDNPLGPVFVRLGDPKLGLGIHGTNAPASVPGVRSHGCVRMKS
                     PDALEFAKTIASGSPASVIYQMAGLNEDADRNLWLAAFRDPYGKNNLDIASLKKSIAQ
                     WAKTQGKTIAPEKVDAVLKDRTGSAVCLTCGKNGKMKMPLKSLAWIQGSSSYSQAEVI
                     EQTEETNSAEISETRTPEVPDVHTPEAQPHLNTQSDGTPTAYTEPAADSSPQVETPDQ
                     AASEPVDVLFSIDVIRQGNLRLGN"
     gene            12781..12918
                     /locus_tag="ACFM8S_000563"
     CDS             12781..12918
                     /locus_tag="ACFM8S_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase"
                     /protein_id="HFC6313680.1"
                     /translation="MATETNVAEISALDYEGRGVAKVGGKTVFIKRALLDCLMLGWFR
                     L"
     gene            12940..14082
                     /gene="bioF"
                     /locus_tag="ACFM8S_000564"
     CDS             12940..14082
                     /gene="bioF"
                     /locus_tag="ACFM8S_000564"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFC6313681.1"
                     /translation="MKVFKQQLEQLGAQNQYRSIPDLIHQGRYITRENCKMLNMSSND
                     YLGLASDENLRRSFLQQYGGNFPSFTSSSSRLLTGNFPIYTDLEELVAQRFQRESALL
                     FNSGYHANLGILPALTTTKSLILADKFVHASMIDGIRLSRCAFFRYRHNDYEHLKNLL
                     EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNTYLYVDEAHAIGVYGQNGL
                     GIAERDNLIAEIDLLVGTFGKALASVGAYAVCNQVLKECLINQMRPLIFSTALPPFNV
                     AWTYFIFERLPQFSKERSHLEQLSAFLRREVAHRTQIMPSQTCIVPYILGGNEAALAK
                     AEYLQRQGYYCLPIRPPTVPKGTSRIRLSLTADMTMDEVRQFAACL"
     gene            14092..14739
                     /locus_tag="ACFM8S_000565"
     CDS             14092..14739
                     /locus_tag="ACFM8S_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl esterase BioG family
                     protein"
                     /protein_id="HFC6313682.1"
                     /translation="MKTKFYNHQGEHLILYFAGWGTPPDAVNHLILPENHDLLICYDY
                     QDLNLDFDFSAYRHIRLVAWSMGVWAAERALQGIRLKSATAVNGTGLPCDDNFGIPCA
                     VFKGTLENLTENTRLKFERRMCGDKASFEDYQQFSARPFDEIHQELIALFAMIGQDRR
                     ADLIRWTNALVGSGDKIFMPANQHRYWTPRCTVREIDGGHYLFSRFTHWSALWNH"
     gene            14727..15509
                     /gene="bioC"
                     /locus_tag="ACFM8S_000566"
     CDS             14727..15509
                     /gene="bioC"
                     /locus_tag="ACFM8S_000566"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFC6313683.1"
                     /translation="MESLTAINKSRIRQAFQKALNDYDRHALIQQKMTINLMAHLQDY
                     LPDMPLENVLELGCGSGMLSALLQKQISANYWLFNDLCDVQPQLAEKLPQSFDFYCGD
                     AENFPFQRQFDLIASASAVQWFHQPDAFIAHCKTGLKANGLLAVATFGKDNLKEVRQI
                     TNIGLNYPTLSQWQTWLAKDFELLWCEDFKVILDFDTPLDVLKHLKYTGVTATNQKNW
                     TRKNLNEFIGDYLSAFSMPSGKVHLTYHPLFFIARYSAAGRQ"
     gene            15729..17165
                     /locus_tag="ACFM8S_000567"
     CDS             15729..17165
                     /locus_tag="ACFM8S_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC6313684.1"
                     /translation="MENSLIIGLALAVLLMLLVMRAKQGKKTPKRKSQGVGNTQGQTP
                     GSNDSDWVDQIGQSVSDGTQPDWSWNESAETASAAVSAQEVDPLTEYQVYKQFGYQGK
                     AAESLAAYLDGIPDGEAKPENLIRELLDINLEVGDVDVLADNLQKYGKLIPSELLAKY
                     IEQALQRDSNHLRIRVLAEEGLGWGTQEIEKRAEGGSATAASASPPPDAGGKAYEAEE
                     IKRIPIVRGKKDVSGISQEEIGAIAGLVRADQGAKILKDKVSYETASKQYDRAIQTSE
                     KPANLIIDALKLDYQHADIDRFAGHLWKLYQTLGNYGRQVKERMLGWGYSLGYHEVFD
                     DLEKGPNDRQIKDIGMGHGYLPKNIQKFKSQHRDLVLQDSSLINTGSSPADDAVKEVE
                     SLLMYGQIEAAMDVLEQAVLKYPDESQLYITLIDIYERTEDWDRLGQFLRVLRERADR
                     LPEEVVMLMSRLLQRMNQNIKKIKRYGK"
     gene            17168..18265
                     /locus_tag="ACFM8S_000568"
     CDS             17168..18265
                     /locus_tag="ACFM8S_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFC6313685.1"
                     /translation="MEVQLPKIKTVRVMLAGMTAQQESVFKMAFKMHNTTRYETVSPS
                     DGSAVPDLVLADTDAEGGFELWKELAERYKDIPVAVCSEKVPDSEVPYLPKPIRFETL
                     FPMLRKLLQGENVYGKSFIAPADRSAKNNGNVQRTVTIRQFNPNKGLLGALRFAEKNR
                     QDIAILHGNKPVLIVFPSIQRVLLTESVQKLEELCKDENLQVSCKTVPDNPQWREKAK
                     VGIMSCMWQFSIWTAQGRLIYPISPDTPFTLKSWPNLTRLANVPGSIRLSAFLTKASV
                     NLNVLYKVMPLNLNDILNYLAATYTTGFLSVDLKTVSQQAYSDMADKINIGADSASDS
                     EMMKKAEKITTSSQSQSRGLLQRLMKKLLGS"
     gene            18277..18813
                     /locus_tag="ACFM8S_000569"
     CDS             18277..18813
                     /locus_tag="ACFM8S_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HFC6313686.1"
                     /translation="MRENKIIFTGPVGVGKTTAIAAISDEALVQTDASASDMTLDRKR
                     NTTVAMDYGAISLDEDTKVHLYGTPGQERFNFMWEILSQGSMGLVLLLDNARTNPLKD
                     LEFFLHSFRGLLEKAPVVVGITKMDIRSQPGIDVYHKYLAKHNLNVPVFEIDARKEDD
                     VKQLVSAMLFSIDPGLEV"
     gene            18815..19459
                     /locus_tag="ACFM8S_000570"
     CDS             18815..19459
                     /locus_tag="ACFM8S_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFC6313687.1"
                     /translation="MESTLSLQANLYPRLTPAGAFYAVSSDAPSAGKTLLHSLLKADV
                     DEMVSSEKLLTWADTADIDTALNLLYRLQKLEFLYGDENGHSDGINLSDEQLPLLMEQ
                     LSGSGKALLVDRNGLYLANANFHHEAAEELGLLAAEVAQMEKKYRLLIKNNLYINNNA
                     WGVCDPSGQSELTFFPLYIGSTKFILVIGGIPDLGKEAFVTLVRILYRRYSNRV"
     gene            19482..19841
                     /locus_tag="ACFM8S_000571"
     CDS             19482..19841
                     /locus_tag="ACFM8S_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HFC6313688.1"
                     /translation="MQQLLISILEDLNNTSTDIIASAVISTDGLPMATMLPSHLNSDR
                     VGAISATLLALGSRSVQELACGELEQVMIKGKSGYILLSQAGKDAVLVLVAKETGRLG
                     LILLDAKRAARHIAEAI"
     gene            19934..20009
                     /locus_tag="ACFM8S_000572"
     tRNA            19934..20009
                     /locus_tag="ACFM8S_000572"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19967..19969,aa:Asn,seq:gtt)
BASE COUNT         5109 a         4671 c         5236 g         5195 t
ORIGIN      
        1 atgtaggttc gtgggaatga cgcggtgcag gtttccgtgc ggatggattc gtcattcccg
       61 cgcaggcggg aatctagaca ttcaatgtta aggcaattta tcgggaatga ctgaaactca
      121 aaaaactgga ttcccacttt cgtgggaatg acgggatgca ggttcgtggg aatgacggga
      181 tttgaggttt ctgtttttga ttttctgttt tcgtgggaat gacggtttag aagttacccg
      241 aaacctcaaa aaaaccgaaa ccgaacggat cggattaccg ctttcgcgtt tcaaagttac
      301 ggcgttatcg gaaaaacaga aaatcaaagc tgtaagaatt tatttaaaac aaccgaattt
      361 caacggatcg gattctcgcc tgtagggaat aacggcggaa ggttttttgt cttttctgac
      421 agatgtccgc aatctgaaat cctgaccgtg ggagcgacgg ttctatttca atccaatata
      481 aaatgccgtc cgaacgatgt tcggacggca tttttaatgc ttcaaatcag ttggtgccga
      541 ctttgatttt ctgccacagg ctgacagaca gttttttcgc atccgtactc atttgcggca
      601 tcacgaaacc gtctttcata tcctgctcgt tcgggaagat ggaacgggtg ttcaccagtt
      661 cggcaggcat tttttcgcgt gccggtttgc tggcgggggc gaaggttacg gcgatgccgt
      721 ttttcgctgc gatttcgggg tcgagcgtgt agttgatgta tttgtgggca ttggcgacgt
      781 ttttcgcatc ggcgggaatc agccaagact caatccagaa gcccatgcct ttcggattca
      841 gcacttcgat gccgacgttg tttttcactt cttcggaacg tgctttcgcc aagttcaaat
      901 cgccgccgtt gcctgccgcc aggcagatgt cgccgcgcgc cagctcgtcg atgatggacg
      961 ggctgaaacg tttgacatcc ggacggatag acttcaacac ttccgccgcc gccttcaaat
     1021 cttcaggatt cgagcctttg gggtctttgc ccaagtagtt cagcaaaatc gggaacattt
     1081 cgctcggggt gtcccacagg gcgatgccgc aggatttcag cttgtgggtg tattcgggtt
     1141 tgaacagcaa atcccagccg ttttcgggca gcttgccgcc taaaagctct ttgcctttcg
     1201 ccgtaatcgc aatcgtgttc acgccggaga aataggggac ggcatactgg ttgcccgggt
     1261 cggcggtttc cagcattttc aagagttcgg gatcgatgtt tttatagttg ggaatcaggt
     1321 ctttgttgac tttttgatac gcgcccgcct cgatttggcg cggcaggaag gcgatgcccg
     1381 gcacaaccaa atcgtaaccg gatttgccgg tcagcatttt ggcttccagc gtttcattgt
     1441 tttcgtacaa gtcgtaagtc agcttcagat tgttggcttt tttaaagtct tcgaccgtac
     1501 tctcgtcaac atagtttgac cagttgtaga tgttcagagt atcggtggca gcggcttcgg
     1561 cattggcagc agacgcagcg tctgcttgag gttgcacggc gtttttttcg ctgccgccgc
     1621 aggctgccag agacagcgcg gccaaaacgg ctaatacgga ttttttcata cgggcagatt
     1681 cctgatgaaa gaggttggaa aaaaaagaaa accccgcgcc ccataaacac cccggcgcaa
     1741 ggtttgggca ttgtaaagta aatttgtgca aactcaaagc gatattggac tgattttcct
     1801 aaaaaattac cctgtttcca aaagaggggg aaaacggccg cacgattttg ccgttttttt
     1861 gcgctgtcag agtgtccgac gggcggatag agagaaaagg cttgcatata atgtaaaccc
     1921 cctttaaaat tgcgcgttta cagaatttat ttttctttca ggagattcca atatggcaaa
     1981 cagcgcacaa gcacgtaagc gtgcccgcca gtccgtcaaa caacgcgccc acaacgctag
     2041 cctgcgtacc gcattccgca ccgcagtgaa aaaagtattg aaagcagtcg aagcaggcga
     2101 taaagctgcc gcacaagcgg tttaccaaga gtccgtcaaa gtcatcgacc gcatcgccga
     2161 caagggcgtg ttccacaaaa acaaagcggc acgccacaaa agccgtctgt ctgcaaaagt
     2221 aaaagcactg gcttgatttt tgcaaaaccg ccaaggcggt tgatacgcga taagcggaaa
     2281 accctgaagc ccgacggttt cggggttttc tgtattgcgg gggcaaaatc ccgaaatggc
     2341 ggaaagggtg cggtttttat ccgaatccgc tataaaatgc cgtttgaaaa ccaatatgcc
     2401 gacaatgggg gcggagatga atacacggaa tatgcgctat attcttttga caggactgtt
     2461 gccgatggca tccgcttttg gagagaccgc gctgcaatgc gccgctttga cggacaatgt
     2521 tacgcgtttg gcgtgttacg acaggatttt tgcggcacag cttccgtctt cggcagggca
     2581 ggaagggcag gagtcgaaag ccgtactcaa tctgacggaa accgtccgca gcagcctgga
     2641 taagggcgag gcggtcattg ttgttgaaaa aggcggggat gcgcttcctg ccgacagtgc
     2701 gggcgaaacc gccgacatct atacgccttt gagcctgatg tacgacttgg acaaaaacga
     2761 tttgcgcggg ctgttgggcg tacgcgaaca caatccgatg taccttatgc cgctctggta
     2821 caacaattcg cccaactatg ccccgggttc gccgacgcgc ggtacgactg tacaggaaaa
     2881 attcggacag cagaaacgtg cggaaaccaa attgcaggtt tcgttcaaaa gcaaaattgc
     2941 cgaagatttg tttaaaaccc gcgcggatct gtggttcggc tacacccaaa gatccgattg
     3001 gcagatttac aaccaaggca ggaaatccgc gccgttccgc aatacggatt acaaacctga
     3061 aattttcctg acccagcctg tgaaggcgga tttgccgttc ggcggcaggc tgcgtatgct
     3121 cggtgcgggt tttgtccacc agtccaacgg acagagccgt cccgaatcgc gttcgtggaa
     3181 caggatttac gccatggcag gcatggaatg gggcaaattg acggtgattc cgcgcgtgtg
     3241 ggtgcgtgcg ttcgatcaga gcggcgataa aaacgacaat cccgatattg ccgactatat
     3301 ggggtatggc gacgtgaagc tgcagtaccg cctgaacgac aggcagaatg tgtattccgt
     3361 attgcgctac aaccccaaaa cgggctacgg cgcgattgaa gccgcctaca cgtttccgat
     3421 taagggcaaa ctcaaaggcg tggtacgcgg attccacggt tacggcgaga gcctgatcga
     3481 ctacaaccac aagcagaacg gtatcggtat cgggttgatg ttcaacgact tggacggcat
     3541 ctgaaccgcg tgttcagacg gtatatcaag ttgccgtgcc gtctgaagcc gctggcggtt
     3601 tggcggggcg ggataaaaat ttcgtttgaa tccgggcaaa cgcgtataat gtgcggtttg
     3661 tacggacttt gtcggaatca agcaagatga cggaacctgc ggccgaaggc ggtaaagctg
     3721 ccaaggcgtt aaaaaaatat ctgattacgg gcattttggt ctggctgccg attgcggtaa
     3781 cggtttgggt ggtttcctat atcgtttccg cgtccgatca gctcgtcaac ctgctgccga
     3841 agcaatggcg gccgcaatat gttttggggt ttaatatccc ggggctgggc gttatcgttg
     3901 ccattgccgt attgtttgta accggattat ttgccgcaaa cgtattgggc cggcagattc
     3961 ttgccgcgtg ggacagcttg ttggggcgga ttccggttgt gaagtccatc tattcgagtg
     4021 tgaaaaaagt atccgaatcg ctgctgtccg acagcagccg ttcgtttaaa acaccagtac
     4081 tcgtgccgtt tccccaatcg ggtatttgga caatcgcatt cgtgtccggt caggtgtcga
     4141 atgcggttaa ggccgcattg ccgaaggacg gcgattatct ttccgtgtat gttccgacca
     4201 cgccgaatcc gaccggcggt tactatatta tggtaaagaa aagcgatgtg cgcgaactcg
     4261 atatgagcgt ggacgaagcg ttgaaatatg tgatttcgct gggtatggtc atccctgacg
     4321 acctgcccgt caaaacattg gcaggaccta tgccgcctga aaaggcggat ttgcccgaac
     4381 aacaataaag ccgccgttca gacggcattt tctgttttca gtttaaatca ataaaaggtg
     4441 attttatgcg taccaactat tgcggcctga tcagcgagca atacttagac caaaccgtta
     4501 ccgtcaaagg ctgggtacac cgtcggcgcg accacggcgg tgtgattttt atcgacctgc
     4561 gcgaccgcga aggcatcgtc caagtcgtga tcgatcccga cacgcccgaa gcgtttgccg
     4621 ctgccgattc ctcccgcaac gaatacgttt tgagcattac cggccgcgta cgcaaccgtc
     4681 cagaaggtac aaccaacgac aaaatgattt ccggcaaaat cgaaatcctt gccaaagaaa
     4741 tcgaagtatt gaacgctgcc gccacgccgc cgttccaaat cgacgacgaa aacatcagcg
     4801 aaaacgtgcg cctgaccaac cgcgttatcg acctgcgccg tccggtgatg caacgcaacc
     4861 tgcgcctgcg ttatcaagtc gctatgggcg ttcgccgcta cttggacgcg caaggcttca
     4921 tcgacatcga aacgccgatg ctgacccgct ccacgcctga aggcgcgcgc gactacctcg
     4981 tgccgagccg cgttcatccg ggcgagtttt tcgcgctgcc tcaatcgccg caattgttca
     5041 aacaactgtt gatggtggcg ggtttcgacc gttactacca aatcaccaag tgcttccgcg
     5101 acgaagactt gcgtgccgac cgtcagcctg aattcacgca aatcgacttg gaaacctcgt
     5161 tcttaaacga ggatgaaatc atggacatca ccgaaggtat ggcgaaacaa gtcttcaaag
     5221 acgcgctggg cgtggacttg ggcgacttcc cacgcatgcc ttactctgaa gccatgttct
     5281 actacggctc tgacaaaccg gacatgcgta tcaacttgaa attcaccgag ttgaccgacc
     5341 tgatgaaaac ggaagaattc aaagtcttcc gtggcgcagc cgacatgaaa ggcggccgcg
     5401 tggttgccct gcgtgtgccg aacggtgcga aattcagccg caaagaaatc gacgaataca
     5461 ctaaatttgt cggcatctac ggtgcgaaag gtctggcata catcaaagta aatgatgtgg
     5521 gcaacctctc caacggcgaa gacagcggcc tgcaatctcc aatcgtaaaa ttcctgtctg
     5581 agaatgccct gaaagaaatc atcgcgcgta ccggcgcaca aaacggcgat attatcttct
     5641 tcggtgcaga caaaaccaaa gtcgtgaacg aagccatcgg cgcattgcgt atcaaagtcg
     5701 gcttggaaca cggcaaagat aacggctact tcacagacga atggaaacct ttgtgggttg
     5761 tcgacttccc aatgttcgaa tacgacgaag aagccgaccg ctacgttgcc gtacaccatc
     5821 cgtttaccgc gccaaaagaa ggtcatgaag acctgatggt ttccgacccg gcaaattgtt
     5881 tggcacgcgc ctacgatatg gtattgaacg gctgggaaat cggcggcggc tctatccgta
     5941 tccaccgtgc agacgtacaa gagaaagtgt ttgccgcgct gaaaatcagc cctgaagagc
     6001 aacaagagaa attcggcttc ctcttggaca acctgaaatt cggtgcgcct cctcacggcg
     6061 gccttgcatt cggcctcgac cgtctggtaa cgctgatgac cggagccgaa tccatccgcg
     6121 acgtgattgc cttcccgaaa acacaacgcg cacaatgcct gctgaccaac gcgcccaaca
     6181 gcgtggacga caagcagttg cgtgaattga gcctgcgcct gcgccaaaag gcggctgaaa
     6241 ccaaagaagc ataaggaaaa cagagccatt tgacggctct gtttttttca gacggcattt
     6301 acgcttctta acttccctct aattcaaacc taattttgct gtgttttaat ggcggtatta
     6361 aaaaacacat cttgttcaat caaccgatta agaaaggact gaaaatgaaa aaactgttat
     6421 tggctgccgt tgtttccctg agtgccgcaa ccgcatttgc cgacgattct gccgagcgtc
     6481 agatttacgg tgatccccat tttgaacaga accgcacaaa agccgtaaaa atgttggaac
     6541 agcgcggtta tcaggttcac gatgtcgatg ccgacggcca ttggggcaaa cctgttttgg
     6601 aagtggaagc ctataaagac ggtcgtgaat acgacatcgt gttgtcttac cccgacctga
     6661 aaatcatcaa agagcagctc gatcgctgac tcctttgata gaaagatgaa ccaaaatgcc
     6721 gtctgaagcg ttcagacggc attttgcctg ttcctcatca ggtatgaggc aggcttttct
     6781 tattaaaaaa atgacatttc acgctgattt gttataatca ttccttttca acacgacaga
     6841 cggagcaggt ttattatgcc tatccttacc atccgtgaag tgtgcaacat taatcattgg
     6901 ggcataggtt attatgatgt tgacgattcc ggcgaaatca tcgtccgccc caatccggcg
     6961 caacacaatc aaactgtttc actgcaaaaa ctgactgaag ccgtgcaaca aaaacatcag
     7021 gcgcgcctgc ctgttttgtt ttgttttccg caaatcctcg aacaccgcct ccgcgacatt
     7081 aaccgcgcct ttcagacggc acgggaagag tgcggctata agggcggtta ttgtttggtt
     7141 taccctatca aggtcaacca acaccgccgc gtcatcgaat cgcttatgtc aagcggacaa
     7201 ccgcatggtt tggaagccgg ttctaaagcc gaactgatgg cggttttggc acacgccggc
     7261 acccggcaaa cattaatcgt ctgcaacggc tataaagacc gtgaatatat ccgttttgcc
     7321 ttgatgggcg aaaaactggg gcatcaggtt tatttggtga ttgagaagct gtccgaaata
     7381 caaatggtat tggaagaggc ggaaaaactc ggcgtcaagc cccgtttggg tgtgcgcgcc
     7441 agactggctt cccaaggttc gggaaaatgg cagtcttcgg gtggggaaaa atcaaaattc
     7501 ggcttgtcgg cttcccaagt tttgcaactg gtcgatattt tgaaacaaaa aaacaggctg
     7561 gattgcctgc agcttttgca tttccatttg ggctcgcagc ttgggaacat ccgtgatgtt
     7621 gccacaggtg tacacgaatc ggctcggttt tatgttgagt tgcacaaact gggggtaaat
     7681 atccgctgtt ttgatgtagg cggcgggctt ggcgtggatt acgaaggaaa ccgcacacaa
     7741 tcggattgtt ccgttaatta cagcctcaac gaatatgccg ccacagtcgt atggggcatc
     7801 agtcaggctt gtctcgaaca cgggctgccg catccgacaa tcatcaccga gagcgggcgc
     7861 ggcattaccg cacatcacgc cgttttggtt gctaatgtta taggcgttga gcgttacaaa
     7921 ccgcgccggc tggatgcgcc atcgcccgaa gcgccgcgtg tgttgcacag tatgtgggaa
     7981 acttggacgg atatttccgc ctcgcgggaa aaacgttcct tacgcagctg gatacacgaa
     8041 gggcagtttg atcttgccga tgtgcataat cagtataatg tcgggctgtt gagtttggcg
     8101 caacgtgcgt gggcggagca actgtattta aatatctgtc atgaagtcgg cgaattgttt
     8161 aatgaaaaac accggtctca ccgaaccatt attgacgaat tgcaagaacg ttttgccgat
     8221 aagctgtatg tcaatttctc actcttccaa tctttgcccg atgcttgggg catagatcaa
     8281 cttttccctg tttgtcccat taccggtttg aatgaaccga ttgcgcgccg cgccgtgttg
     8341 ttggacatta cctgcgattc agacggtacg attgaccact acatcgacgg cgacggcatc
     8401 gccggtacga tgcctatgcc tgattatccc gaagaagagc cgccgctttt aggctttttt
     8461 atggtgggag catatcagga aatactcggc aatatgcaca atcttttcgg cgacactgcc
     8521 actgccgatg ttgttgtagg ggaagacgga caatttaccg tcatcgatta cgatgaagga
     8581 aacaccgttg ccgatatgct cgaatacgtt tatcaagatc cgaaagagct gatgaaacgc
     8641 tatcgcgaac aaatcgaaca ttcagacctt cctgcctcgc aggctatgtc tttcttaaaa
     8701 gaactcgaag cggggcttaa tggttatacc tatttggaag acgaatagac gcatcaaggc
     8761 atcggatatg tcgtctgaag cccgattttc ttactcaaac accaatcatc acgaccgatt
     8821 gaaaccaatt acaaggaatc attacgatgc aatacagcac actggcagga caaaccgaca
     8881 actccctcgt ttccaataat ttcgggtttt tgcgcctgcc gcttaatttt atgccgtatg
     8941 aaagtcatgc cgattgggtt attaccggtg tgccttatga tatggcggtt tcagggcgtt
     9001 ccggcgcgcg tttcggtcct gaagccatcc ggcgcgcctc cgtcaacctc gcttgggagc
     9061 accgcaggtt tccatggaca tttgatgtgc gcgaacgcct gaacattatt gattgcggcg
     9121 acttggtttt ttcttttggc gacagcaggg attttgtcga aaaaatggaa gcgcacgccg
     9181 gcaaattact ttcttccggc aaacgctgtt tgagtttggg cggcgaccat ttcattaccc
     9241 tcccgttgtt gcgcgcccac gcccgctatt tcggcaaact cgcactgatt cattttgacg
     9301 cgcacaccga cacctacgac aacggcagcg aatacgacca cggtacgatg ttctataccg
     9361 cccccaagga aggcctcatc gacccgtccc gttccgtaca aatcggcata cgcaccgaac
     9421 acagtaaaaa attgcctttt actgtgttgt ccgcccccaa agtcaatgaa gacagtgttg
     9481 aagagaccgt ccgtaaaatc aaagaaaccg tcggcaatat gcccgtttac ctgactttcg
     9541 acatagactg cctcgacccg tcgttcgccc ccgggaccgg tacgcccgta tgcggcggct
     9601 tgagcagcga cagggcatta aaaatcctac gtgggctgac ggatctcgac atcgtcggta
     9661 tggatgttgt agaagttgcc ccctcttacg accaatccga cattaccgct ttggccggtg
     9721 ccacaattgc cttggaaatg ctttaccttc aaggtgcgaa aaaggactga acgtccggca
     9781 tcccccgggt tttcgccgtg ccgttcaaac ggcgtattca gtctaatgaa aattcaaata
     9841 ctgaaacaaa agttgcccgg agccgcatat cggaaagacg gtgaaatatc ggaatatatc
     9901 ttataaaaca attagttaaa tattattttt ccgatttttc gggacggtct tttttacgga
     9961 ggtcaatatg atgaaattgg gtttcaaacc gatacccctc gccattgccg cagtattgtg
    10021 cgccctggtt ttggcactgc ccgtacccga cggggtcaag cctcaggctt ggacgctgct
    10081 ggccatgttt gtcggtgtga ttgccgccat tatcggcaag gccatgccgt tgggcgcgct
    10141 gtcgattatt gccgtcgggt tggtcgcagt aaccggcgta accgccgaca aaccgggcgc
    10201 ggcgatgagc gatgcgttga gtgcgttcgc caatccgttg atttggctga ttgccatcgc
    10261 agttatgatt tcgcgcggtt tgctcaaaac agggctgggg atgcgtatcg gatatttgtt
    10321 tatcgccgtt tttggaagaa aaacgctggg catcggttac agtctcgctc tttccgaact
    10381 gctgctggct cccgttaccc cttccaatac cgcgcgcggc ggcggcatta tacatccgat
    10441 tatgcagtcg attgccggca gttacggctc caatcccgca aaaggcacag aaggcaagat
    10501 gggtaaatat ttggctttgg tcaactatca ttccaatccc atttcgtcgg ctatgtttat
    10561 tactgcaact gcccccaacc ctttaatcgt caacttgatt gccgaaaatt taggcagtag
    10621 tttccgtctt tcttgggggg cgtgggcgtg ggcaatggct gttcccggcg ttatcgcctt
    10681 tttcgttatg cctttgattt tatatttttt gtatccgcct gaaattaaag aaacgcccaa
    10741 tgccgttcaa tttgccaaag accgtctgag ggagatgggt aaaatgtcgg cagacgaaat
    10801 cattatggcg gtcattttcg gtatcttgtt gctgttgtgg gcagatgttc ccgcccttat
    10861 taccggcaat cacgctttta gtatcaacgc caccgccacc gcatttatcg gattaagcct
    10921 gcttttgctt tccggtgtat tgacttggga cgatgttttg aaagaaaaaa gcgcgtggga
    10981 tacgattatt tggtttggcg cattgattat gatggccgca tttttaaata aactcggact
    11041 gattaaatgg ttctccggag tgttggcgga aagtgtcggc ggtttgggcg ttagcggcac
    11101 ggctgcgggc gtaatcctcg tgcttgctta tatgtatgcg cattatatgt ttgccagtac
    11161 tactgcacat attaccgcta tgttcggcgc atttttcgct gctgccgttt cactgaatgc
    11221 cccggcgatg ccgaccgcgc tgatgatggc ggccgcatcc aacattatga tgaccctcac
    11281 tcattatgcg accggtactt cgcctgtgat tttcggttcg ggctacacca caatgggaga
    11341 atggtggaag gcgggtttta tcatgagcgt agtcaatttt ctgatttttt tcgttatcgg
    11401 cagcatttgg tggaaagttc tggggtattg gtaagggaaa aataaaataa atttccaatc
    11461 tgtgtttatt tgattgggcg actattatcg tgaaatatgc cgtctaaagc cttcagatgg
    11521 catatttgtg cgcttgaatg ttgcagaaag cggcaggcgg cggtgtagga aaagccaaac
    11581 aaaaaccaaa ccgcctatca acttctgata aacataagca ttaaataatc agaaggttat
    11641 tcaattacct aaacgcaaat ttccctgccg tatcacatct attgaaaata atacatcaac
    11701 cggctcggaa gctgcctgat caggtgtttc tacttgcggc gatgaatcgg cagccggttc
    11761 ggtataggca gtcggcgtgc cgtcggattg ggtatttaaa tgcggttgtg cctcgggcgt
    11821 gtgtacatca ggcacttcgg gcgtgcgtgt ctcggatatt tcggcagagt tggtttcctc
    11881 agtttgttca atgacttcag cttggctgta tgaggaagaa ccctgtatcc acgccagcga
    11941 tttgagcggc atcttcatct tgccgttttt gccgcaggtc aggcagacgg ccgatccggt
    12001 gcggtctttg agtacagcat cgactttctc gggcgcgatg gttttaccct gtgtttttgc
    12061 ccattgcgca atactttttt tcagagaggc gatgtcaagg ttgttcttac cgtaagggtc
    12121 gcggaaggcg gcaagccaca ggttgcgatc cgcatcttca ttgagacccg ccatttgata
    12181 gatgacggag gcgggcgagc cgctggcgat ggttttggca aactcgagcg cgtcgggcga
    12241 tttcatgcgg acgcagccgt gactccgaac cccggggacg ctggccggcg cattggtccc
    12301 gtgtataccc aaaccgagtt tggggtcgcc taagcggaca aaaaccggcc ccaaagggtt
    12361 gtccgggccg gcggctatgg tttttacgcc gtcgccgcgt tctttctgta tggatttggg
    12421 gatgtaccaa acagggttat aggctttcgc accgatttta tgttcgccta gattggtttg
    12481 cgtcatcgcc cgacctactg caacgggata aaccttggtc agtttgccgt cggtgtagag
    12541 gaacaggcgt tgctgaggga tgttaatgaa gacatgttga ccttgtgcga cgggggagac
    12601 atcgggaatg acggtgtttg cgtatgaaaa accgcttatc aatagtgcag cagtgcggca
    12661 gattgtttta ttcatatcaa aatatggtgt gtgtccgata ggttttcggc aaatcatacc
    12721 tgaaaccgta ccaatttgtg cgaaaatatg cgcttcggta cagtgcggac ggattgggta
    12781 atggcaacgg aaacaaatgt cgcggaaatt tccgccttgg attatgaagg caggggtgtg
    12841 gcaaaggtcg gcggcaaaac ggtttttatt aaaagggcat tacttgattg tttgatgctg
    12901 ggttggttca ggctttaact caggaatatt tacatcataa tgaaggtttt taaacaacag
    12961 cttgaacaac tcggcgcgca aaaccaatat cgttcgattc cggatttgat tcatcaaggg
    13021 cggtatatta cgcgggaaaa ctgcaaaatg ctgaatatgt cgtctaatga ttatttgggt
    13081 ttggcatcag atgaaaactt gcgccggtct tttttgcagc aatacggcgg taattttccc
    13141 tcttttacca gttcttcatc gcgtttatta acgggcaact ttcctattta taccgatttg
    13201 gaagagcttg tcgcacaacg tttccaacgg gaaagcgcgt tattgttcaa cagcggctat
    13261 cacgccaatc tcggtatttt gcctgctttg acgacgacga aaagtttgat tttggcagat
    13321 aaatttgttc acgccagtat gattgacggc atccgtttga gccggtgtgc gtttttccgt
    13381 tatcgtcata atgattatga acatttgaaa aatctgcttg aaaaaaacgt cggaaaattt
    13441 gaccgcactt ttatcgttac cgaatctgtt ttcagtatgg acggcgatgt ggcggatttg
    13501 aaacagcttg tccaattaaa aaaacagttt cccaatactt atctttatgt ggatgaagcc
    13561 cacgcaatcg gtgtttatgg gcaaaacgga ttggggattg ccgaacggga taatttgatt
    13621 gccgagattg atttactggt cggcactttc ggtaaagcct tagcctcggt gggggcgtat
    13681 gccgtctgca accaagtatt gaaagaatgt ttgattaatc aaatgcgtcc attgattttt
    13741 tcaaccgcat tgccgccgtt taatgtggct tggacttatt ttatttttga acgattgccg
    13801 caattctcaa aagaaagaag ccatcttgag cagttaagcg catttttacg gcgggaagtg
    13861 gcgcatcgga cgcaaataat gccgagccaa acctgtatcg tcccctatat tttaggcggg
    13921 aatgaagccg cccttgccaa agcggaatat ctgcaaaggc agggttatta ttgcctgccc
    13981 atcagaccac cgacagtacc aaaaggtaca tccagaatac gattgtcttt gacggcagat
    14041 atgacaatgg atgaagtgcg gcagtttgcg gcgtgcctgt aaggatatga tatgaaaaca
    14101 aaattttaca atcatcaagg cgaacattta atcctgtatt ttgcaggttg gggaacgccg
    14161 cccgatgctg taaatcattt gattttgccg gaaaatcacg atttattgat ttgctatgat
    14221 tatcaagatt taaatttgga ttttgatttt tccgcctatc ggcacatccg tttggtggcg
    14281 tggtcaatgg gcgtttgggc ggcagagagg gcattgcaag gaataagatt aaaatccgca
    14341 acggcagtga atggcacagg tttgccttgc gatgataatt tcggtatccc ttgcgccgtt
    14401 tttaaaggca cattggagaa cctcacggaa aacacccgtt taaaatttga acgcagaatg
    14461 tgtggcgata aagcatcttt tgaagattac caacaatttt ccgcccgtcc gtttgacgaa
    14521 attcatcaag aacttatcgc actttttgcg atgatcgggc aagataggcg cgcagatttg
    14581 attcgctgga caaatgcctt ggtcggatcg ggcgataaaa tttttatgcc tgccaatcag
    14641 caccgatatt ggacaccgcg ttgcaccgtt cgggaaattg acggcggaca ttacctgttt
    14701 tcaagattca cccattggtc ggcactatgg aatcactgac tgccataaat aaatcgcgca
    14761 ttcggcaggc tttccaaaaa gcattaaacg attatgaccg gcacgcctta atccaacaaa
    14821 aaatgacgat taatctaatg gcgcatttgc aagattattt gccagatatg ccattggaaa
    14881 acgtgttgga attgggctgc ggctcaggaa tgttgagtgc cttgctgcaa aaacagattt
    14941 cagcgaatta ttggttattt aatgatttgt gcgatgtgca gccccaactg gctgaaaaac
    15001 tgccgcaatc ctttgatttt tattgcggcg atgcggaaaa ctttcctttt caacgacaat
    15061 ttgacttaat cgcaagcgca tctgccgtgc aatggtttca tcaacccgac gcttttatcg
    15121 cccattgcaa aacaggcttg aaagcaaacg gattattggc ggttgcaacc tttggcaaag
    15181 acaatttaaa agaagtccgc caaattacaa atataggctt aaattacccg actttatccc
    15241 aatggcagac ttggttagcc aaagattttg agcttttatg gtgtgaggat tttaaggtaa
    15301 tactagactt tgatacgccg ttagatgtac tcaaacacct taaatataca ggcgtaacag
    15361 ccacgaacca aaaaaattgg acaagaaaaa atctcaatga atttattggc gattacttgt
    15421 cggcgttcag tatgccgtcg ggcaaagtgc acctgactta tcatccgtta ttttttatcg
    15481 cgcgctactc tgcggcgggc aggcaataag gcagcttatg ggcaaagtta tttttatatc
    15541 gggtattgat actgatgtgg gtaaaaggta atatggcgag gcttgtgcag aaggcatatt
    15601 gttaaacgtt aaattatggt atgatttaaa acttacaagt ctatttcagt aaatcgttaa
    15661 taataaaagc ggacaatggc cgttgcaggc ggtcagctat ccgctcgtta cggtatcggg
    15721 agtatccttt ggaaaactca ttaattatcg gtttggcact ggcggtattg ctgatgcttt
    15781 tggttatgcg tgcgaagcag ggtaagaaaa caccgaagcg caaatcccaa ggtgtcggca
    15841 atacgcaggg gcagacaccc ggaagcaatg attcggactg ggtcgatcag attggacaat
    15901 cggtttcaga cggcacgcaa cccgactggt cttggaacga aagtgccgag accgcatccg
    15961 ccgccgtatc cgcgcaagaa gtcgatccgc ttacggagta tcaggtttat aagcaattcg
    16021 gttatcaggg caaggctgcc gaatctttgg ctgcctatct ggacggcatt ccggatggtg
    16081 aagcgaaacc tgaaaacctt atccgcgagc tgctcgatat caatctcgaa gtgggggatg
    16141 tcgatgtttt ggcagacaat ctgcaaaaat acggcaaact gattccttcc gaacttttgg
    16201 caaaatatat cgaacaggca ttacagcgcg attcaaacca tttgcgtatc cgcgtcttgg
    16261 cagaagaggg tttgggatgg ggtactcagg agattgaaaa acgtgcggaa ggcggttctg
    16321 cgacggcagc ttccgcatcg cccccaccgg atgccggcgg taaggcttat gaagccgaag
    16381 aaatcaagcg catcccgatt gtgcggggca aaaaagacgt gtccggaatc agtcaagagg
    16441 aaatcggtgc gattgccggt ctggttcgtg ccgatcaagg tgcgaaaatc cttaaagaca
    16501 aagtcagcta tgaaacggca tcgaaacaat acgaccgtgc catccaaact tccgaaaaac
    16561 ctgcaaacct gattatcgat gcgttgaaac tcgattacca acacgcggac atagaccgtt
    16621 ttgccggaca tttgtggaaa ctttaccaaa cgttgggcaa ctacggcagg caggttaaag
    16681 agcggatgct ggggtggggg tacagcttgg gttaccatga agttttcgat gatttggaaa
    16741 aagggccgaa cgaccggcaa atcaaagaca tcggtatggg gcacgggtat ctgccgaaaa
    16801 atatacagaa attcaaatcg caacatcggg atttggtgct tcaagattct tcgttgatca
    16861 acaccggttc gtctccggca gacgatgcgg ttaaggaagt agagtcgttg ctgatgtatg
    16921 gtcagattga agcggcaatg gatgtgttgg agcaggcggt attgaaatat cccgacgagt
    16981 cccagcttta tattacgttg atcgatattt atgaacgtac tgaagattgg gataggttgg
    17041 ggcagttttt aagggtattg agggaacgtg cggacaggct tcctgaagag gtcgttatgc
    17101 tgatgagccg gctgctgcag cgtatgaatc aaaatattaa aaaaataaaa cggtacggaa
    17161 aataaaaatg gaagttcaac tgccgaaaat taaaacagta cgcgtaatgt tggcggggat
    17221 gacggcgcag caggaatccg ttttcaaaat ggcattcaaa atgcacaata ccacccgtta
    17281 tgaaacagta tccccttcag acggcagtgc cgtgcccgat ttggttttgg cggataccga
    17341 tgccgagggc ggttttgaac tttggaaaga gcttgccgag cgttataagg atatacccgt
    17401 cgccgtctgt tcggagaaag ttcccgattc tgaagttccc tacctgccca aaccgattcg
    17461 gtttgaaaca ttgtttccta tgctccgcaa attgttgcag ggcgagaatg tttatgggaa
    17521 atcgtttatt gcacccgcag accggtcggc gaaaaataac gggaatgtgc agcgtacggt
    17581 tacgatacgc cagtttaacc cgaataaagg attattgggg gcgttgcggt ttgcggaaaa
    17641 gaacaggcag gacatcgcta tcttgcatgg aaataagccg gtccttattg ttttcccctc
    17701 gatacaacgg gttttgctga cagaaagtgt gcaaaaactc gaagaattgt gcaaagacga
    17761 aaatttgcag gtcagctgca agactgttcc cgataacccg caatggcgcg aaaaggctaa
    17821 agtaggcatt atgtcctgta tgtggcagtt ttccatttgg acagcgcagg gcaggttgat
    17881 ttatccgatt tctcccgata ctccgtttac gttgaaatct tggccaaacc tgacccggtt
    17941 ggcaaatgtg ccggggtcga tacgcttgtc ggcatttctg accaaggcat ccgtcaacct
    18001 taacgtgttg tataaagtga tgcctttaaa cctcaatgat attctgaatt atcttgcggc
    18061 aacctataca accgggtttt tgtcggtaga tttaaaaacg gtttcacaac aggcatactc
    18121 cgatatggcg gataaaataa atatcggagc cgattctgcc tctgatagtg aaatgatgaa
    18181 aaaagcggaa aaaatcacaa catcatccca atcccagtcg cgcggtcttc tgcaaaggct
    18241 gatgaaaaaa ctgttgggca gctaagaggc ggagagatga gagaaaataa aattattttc
    18301 acaggacctg tcggcgtagg gaaaaccact gccattgcgg ctatttcgga cgaagcactc
    18361 gttcagaccg atgcttccgc atccgatatg actttggata ggaaaaggaa tacgacagtg
    18421 gcgatggact acggggccat cagcttggat gaggatacca aagtccattt atatggtacg
    18481 cccggtcagg aacggttcaa ctttatgtgg gaaatcttaa gccaaggcag tatgggtttg
    18541 gtcttgcttt tagataatgc ccgaaccaat ccgttgaaag atttggaatt ctttttacat
    18601 tcgtttcgag ggctgctgga gaaggcaccc gtcgttgtcg gtattaccaa gatggatata
    18661 cgctctcagc ccggtatcga cgtgtatcac aaatatcttg caaaacataa tcttaatgtt
    18721 ccggtttttg aaattgatgc ccgtaaggaa gatgacgtaa aacaattggt tagcgcaatg
    18781 ttattttcta ttgatccggg actggaggtt taatatggaa tcaacacttt cactacaagc
    18841 aaatttatat ccccgcctga ctcctgccgg tgcattttat gccgtatcca gcgatgcccc
    18901 cagtgccggt aaaactttgt tgcacagcct gttgaaagcg gatgtggacg aaatggtcag
    18961 cagtgagaag ctgcttacct gggcggacac cgccgacatc gataccgctt tgaacctgtt
    19021 gtaccgtttg caaaaactcg aattcctcta tggcgatgaa aacggtcatt cagacggcat
    19081 caatttgtcg gacgagcaat tgccgttgct gatggaacaa ttgtccggca gcggtaaggc
    19141 gttattggtc gatcggaacg gtctgtatct tgccaacgcc aatttccatc atgaggcggc
    19201 ggaagagttg gggttgttgg cggcagaagt cgcacagatg gaaaagaaat accggctgct
    19261 gattaagaac aacctgtata tcaacaataa cgcttggggc gtttgcgatc cttccggtca
    19321 gagcgaattg acatttttcc cattgtatat cggttcaacc aaatttattt tggttatcgg
    19381 cggcattccc gatttgggca aagaggcatt tgttactttg gtaaggattt tataccgccg
    19441 ttacagcaac cgcgtgtaaa acttgggaga gaggaggggt tatgcagcaa ttattgattt
    19501 caatccttga agatttaaac aatacatcta cggatattat cgcgtctgcc gttatctcaa
    19561 ccgacggatt gccgatggcg acaatgcttc cttcacattt gaattcggac agggtagggg
    19621 cgatttctgc cactttgctt gctttgggga gtcgctcggt gcaggaactc gcctgcgggg
    19681 aattggaaca agtgatgatt aaaggaaaat caggctatat ccttttaagt caggcgggta
    19741 aagatgccgt gttggtgctg gtggcaaaag aaaccggcag acttggttta atcctattgg
    19801 atgccaaacg tgcggcaagg catattgcgg aagccatata acatataaag attgcgggct
    19861 tgcagataaa gtgcaatcga ttgtcaattt atattgacac gttcggtatt tctgttttat
    19921 tattcgcgct tattccccga tagctcagtc ggtagagcga cggactgtta atccgcaggt
    19981 ccctggttcg agcccaggtc ggggagccaa atttcaaaac cctctaagta ttttcttaga
    20041 gggttttgtt ttaccggcgg tcagaaacgc atttttgaga tgattgtttt gagatggaat
    20101 aaaatctttg caaaattcct ttcgtgatgg ttatgaaaaa ataggggctg tcctggacag
    20161 ctaggataaa ctcgatttta tagtggatta acaaaaacca gtacggcatt g
//
LOCUS       DAXHSF010000020        19092 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.020, whole genome shotgun sequence.
ACCESSION   DAXHSF010000020 DAXHSF010000000
VERSION     DAXHSF010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19092
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.020"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(<1..128)
                     /locus_tag="ACFM8S_000573"
     CDS             complement(<1..128)
                     /locus_tag="ACFM8S_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFC6313689.1"
                     /translation="MKEYKVVIYQESLLSSLFFGAAKVNPVNFSAFLNKQTPEGWRV"
     gene            complement(203..1546)
                     /gene="argG"
                     /locus_tag="ACFM8S_000574"
     CDS             complement(203..1546)
                     /gene="argG"
                     /locus_tag="ACFM8S_000574"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFC6313690.1"
                     /translation="MNQNHTILQNLPVGQKVGIAFSGGLDTSAALLWMKLKGALPYAY
                     TANLGQPDEDDYNAIPKKAMEYGAENARLIDCRAQLAHEGIAAIQCGAFHVSTGGIAY
                     FNTTPLGRAVTGTMLVSAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPALKIYKPW
                     LDQQFIDELGGRHEMSEFLIANGFNYKMSVEKAYSTDSNMLGATHEAKDLEFLNSGIK
                     IVKPIMDVAFWDENVEIKPEEVSVRFEEGVPVALNGKEYADPVELFLEANRIGGRHGL
                     GMSDQIENRIIEAKSRGIYEAPGMALFHIAYERLVTGIHNEDTIEQYRINGLRLGRLL
                     YQGRWFDSQALMLRETAQRWVAKAVTGEVTLELRRGNDYSILNTESPNLTYQPERLSM
                     EKVEDAAFTPLDRIGQLTMRNLDITDTRVKLGIYSQSGLLSLGEGSVLPQLGNKQ"
     gene            1671..2471
                     /locus_tag="ACFM8S_000575"
     CDS             1671..2471
                     /locus_tag="ACFM8S_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFC6313691.1"
                     /translation="MNAFNLIIIGDEILHGSRQDKHFVFFKSLLESKGLKLNQVQYLP
                     DEPDLLVKQLRRSFSDGMPTFVTGGIGATPDDHTRQAAAAALDLPVVRHAQAAGFIEG
                     VTLKRGEPLDSPEHAQRLKMADFPQGAELVPNPFNNIAGFSIREHYFFPGFPVMAHPM
                     AEWVLETYYADRFNQTQRGSRSVYVFEQPESRITPLMEHIEQTYPGVRSYSLPSVGWT
                     HSDGTQVKPHIEFGIKAEGEAVNLLDAAWAEVLHSLDGLGAELKNRVN"
     gene            2511..3497
                     /locus_tag="ACFM8S_000576"
     CDS             2511..3497
                     /locus_tag="ACFM8S_000576"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFC6313692.1"
                     /translation="MQYRIRRENEAPEAKNTGETLWERDIMREVLLSAYQDRRRERMW
                     KNIWRAVSTLILVALIAGIFRKDEAALQLAGNTPHTAVVNLYGEIGNGVEDQVKKLKD
                     GMEAAYKNPQAKAIVIRANSPGGSPVVSNTAFEEIRRLKAQHPGIPVYLVAEDMCASG
                     CYYIAAAADKIYADPSSIVGSIGVIGSSFDATGLMEKIGVKRRVKIAGSNKGMGDPFS
                     PETPEQSKIWEEMLTGIHGEFIKAVKTGRGGRLKFRQYPDVFSGRVYTGADALKVGLV
                     DGLGNIYSVARDVVKAPDVVDYTPKDDFGRILGRRFGAELKASVREALQAVR"
     gene            3546..4073
                     /locus_tag="ACFM8S_000577"
                     /pseudo
     CDS             3546..4073
                     /locus_tag="ACFM8S_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684692.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            4153..4415
                     /locus_tag="ACFM8S_000578"
                     /pseudo
     CDS             4153..4415
                     /locus_tag="ACFM8S_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557955.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4453..4827)
                     /locus_tag="ACFM8S_000579"
     CDS             complement(4453..4827)
                     /locus_tag="ACFM8S_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HFC6313693.1"
                     /translation="MIIQNEFNLYPSNMLPERFCYPEKYVRISNDTSLIPYIQPHNFH
                     WWFENYGTEGAEVAYIFRNSILPDLNLIPFASNGEWEAYFDGNDVTGNPRVIVINLDN
                     IENHEFFNSFEDWLELAIKDTW"
     gene            complement(4824..4952)
                     /locus_tag="ACFM8S_000580"
     CDS             complement(4824..4952)
                     /locus_tag="ACFM8S_000580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313694.1"
                     /translation="MPQTWGEAIQFRIKKQIENELAPPNWSTQFPNGSIYDPKVTK"
     gene            complement(5000..5356)
                     /locus_tag="ACFM8S_000581"
     CDS             complement(5000..5356)
                     /locus_tag="ACFM8S_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMP12 family DNA mimic protein"
                     /protein_id="HFC6313695.1"
                     /translation="MNEHNLLIFCLKDNVSISEYTEMIDWAYKNIQSETVVEITENQI
                     IEYQNRGLWGLVSEITDNWLFGPSEGDWLIDKESILAVKEKLQNSDFSTEPLVKNIIH
                     ALEYAIKNEKTVIFHF"
     gene            complement(5361..5462)
                     /locus_tag="ACFM8S_000582"
     CDS             complement(5361..5462)
                     /locus_tag="ACFM8S_000582"
                     /inference="COORDINATES: protein motif:HMM:NF018014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313696.1"
                     /translation="MDDIRGIIQGAVNPLIYKVSKQKIIYNKLISGT"
     gene            complement(5496..5839)
                     /locus_tag="ACFM8S_000583"
                     /pseudo
     CDS             complement(5496..5839)
                     /locus_tag="ACFM8S_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688154.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5998..6399)
                     /locus_tag="ACFM8S_000584"
     CDS             complement(5998..6399)
                     /locus_tag="ACFM8S_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313697.1"
                     /translation="MKLKSLDFPTGYFYFDNAAINSDKVEVIAVGYRNTDKTIKIFIE
                     DVIHFRVVDESYFIDTFMDLISEDADRALLHENGGQSFFELLDECYAEWILKESYFPL
                     NREFFKYYIFMFEQTFIEIIGSSATYSIIEG"
     gene            complement(6403..6855)
                     /locus_tag="ACFM8S_000585"
     CDS             complement(6403..6855)
                     /locus_tag="ACFM8S_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313698.1"
                     /translation="MQTKALGTVDEIGDTVQQVGKQASGQKTSGGNPAIDSDPYSPSS
                     VAARIETGKVRSDLQIKDILSNTTQRSKTKGPAVQYDKVGDYNDALNDFNSLNVRNVQ
                     TRPNGTITGNLPDRRAVNARNDSSGGEPTLEITISNNRKIKIRYGNTR"
     gene            complement(6850..7143)
                     /locus_tag="ACFM8S_000586"
                     /pseudo
     CDS             complement(6850..7143)
                     /locus_tag="ACFM8S_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235291.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(7213..7530)
                     /locus_tag="ACFM8S_000587"
     CDS             complement(7213..7530)
                     /locus_tag="ACFM8S_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313699.1"
                     /translation="MKKNIFHNVSLYEIIFSDNGNTLTLSFTDTIEGNYFGYIKCSNI
                     LNFKLDTNNFVDYEDKEDSLFPLFIPEIELYKYQFYSEIIIDVGIIIKISAETINFEP
                     LGK"
     gene            complement(7527..7787)
                     /locus_tag="ACFM8S_000588"
     CDS             complement(7527..7787)
                     /locus_tag="ACFM8S_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFC6313700.1"
                     /translation="MRPTTRDELRQALQEQGFRRTGSDEAQYETWKGPDGVKIDIRPN
                     GEVIRTQRVPRTDGVQGKYPQRQDYEGNPLPNNRHHSGYFVK"
     gene            complement(7785..8129)
                     /locus_tag="ACFM8S_000589"
                     /pseudo
     CDS             complement(7785..8129)
                     /locus_tag="ACFM8S_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225727.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(8235..8606)
                     /locus_tag="ACFM8S_000590"
     CDS             complement(8235..8606)
                     /locus_tag="ACFM8S_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HFC6313701.1"
                     /translation="MIKQNSFVPYPEAMLPKGFKYPQSYLKLAQSTHAINYDEQYSFP
                     WWFENAESNISEVIDIYFEITGIPNLLPFARNQEWAACFDISDKSGNPKIIVVNLDNT
                     KYYETFENFDTWLKEAENDGW"
     gene            complement(8603..8958)
                     /locus_tag="ACFM8S_000591"
                     /pseudo
     CDS             complement(8603..8958)
                     /locus_tag="ACFM8S_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504007.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8986..9192)
                     /locus_tag="ACFM8S_000592"
     CDS             complement(8986..9192)
                     /locus_tag="ACFM8S_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313702.1"
                     /translation="MNILSINNQNSTISLTQDEVFVLRAILNEIYAGVCVDSREFENV
                     SGVRKHEVDNLQQQFAGIYKKMTT"
     gene            complement(9202..10047)
                     /gene="mafB"
                     /locus_tag="ACFM8S_000593"
                     /pseudo
     CDS             complement(9202..10047)
                     /gene="mafB"
                     /locus_tag="ACFM8S_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246532.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            complement(10144..10356)
                     /locus_tag="ACFM8S_000594"
     CDS             complement(10144..10356)
                     /locus_tag="ACFM8S_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313703.1"
                     /translation="MKLINFEESSVKISLDKDELYIIQAIVGEIYSGVCVDCRDFEII
                     HGVEKIKFFLWIKNLKKYMILGINVS"
     gene            complement(10432..10791)
                     /locus_tag="ACFM8S_000595"
     CDS             complement(10432..10791)
                     /locus_tag="ACFM8S_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HFC6313704.1"
                     /translation="MRLKLKNYDCSDFDLEDFPQDKLENFCILLTLSIGFDESNGADY
                     FYVYIYSTEWLLSNIHRPMSLKNSIVTNRFNIEHILKLINDILEICNSTSEDKSISNL
                     AKYFDWEFDDYNLNIQD"
     gene            complement(10796..11512)
                     /locus_tag="ACFM8S_000596"
                     /pseudo
     CDS             complement(10796..11512)
                     /locus_tag="ACFM8S_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504008.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(11729..12112)
                     /locus_tag="ACFM8S_000597"
     CDS             complement(11729..12112)
                     /locus_tag="ACFM8S_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313705.1"
                     /translation="MMTEPHGYPSNNLPVKIINDIIYATQLVEDLVLGKIKIVDFLKS
                     YNNFYYWLGFDELPQSEKIKFLNYLNILSIHKEIQDKTVNRVYTDCFDIDKLHSLGRI
                     TTNECINGIKKIYQKNKLEFDNILK"
     gene            complement(12078..13523)
                     /gene="mafB"
                     /locus_tag="ACFM8S_000598"
     CDS             complement(12078..13523)
                     /gene="mafB"
                     /locus_tag="ACFM8S_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFC6313706.1"
                     /translation="MNLPIQKFMMLFAAAISLLQIPISHANGLDARLRDDMQAKHYEP
                     GGKYHLFGDGRGSVKNRVYAVQTFDATAVGPILPITHERTGFEGVIGYETHFSGHGHE
                     VHSPFDHHDSKSTSDFSGGVDGGFTVYQLHRTGSEIHPEDGYDGPQGSDYPPPGGARD
                     IYSYYVKGTSTKTKTNIVPQAPFSDRWLKENAGAASGFFSRADEAGKLIWESDPNKNW
                     WGNRMDDIRGIIQGAANPFLTGFQGLGVGAITDSAVNPVTYAAARKTLQGIHNLGNLS
                     PEAQLAAATALQDSAFAVKDSINSARQWADAHPNITATAQTALSVAEAAGTVWRGKKV
                     ELNPTKWDWVKNNGYKKPAVRLMQTVDGEMAGGNKPPKPSTQQHSTHSDNNIGLPAPY
                     VKPDTSISPTGTIYVDSKGNAIPTPIGGYITGSPDARFIQVRDSNGNLTGARIDDAHH
                     ANTHQDPRALTGHAHVPGVTNDDGTPWLPIK"
     gene            complement(13563..14525)
                     /gene="mafA"
                     /locus_tag="ACFM8S_000599"
     CDS             complement(13563..14525)
                     /gene="mafA"
                     /locus_tag="ACFM8S_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HFC6313707.1"
                     /translation="MQARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAAS
                     ARAAVKDMDLQALHGRKVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDY
                     TYPRYETTAETTSGGLTGLTTSLSTLNAPARSRTQSDGSGSKSSLGLNIGGMGDYRNE
                     TLTTNPRDTAFLSHLVQTVFFLRGIDVVSPANADTDVFINIDVFGTIRNRTEMHLYNA
                     ETLKAQTKLEYFAVDRTNKKLLIKPKTNAFEAAYKENYALWMGPYKVSKGIKPTEGLM
                     VDFSDIRPYGNHTGNSAPSVEADNSHEGYGYSDEVVRQHRQGQP"
     gene            complement(14709..15428)
                     /gene="pyrH"
                     /locus_tag="ACFM8S_000600"
     CDS             complement(14709..15428)
                     /gene="pyrH"
                     /locus_tag="ACFM8S_000600"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFC6313708.1"
                     /translation="MTQQIKYKRVLLKLSGESLMGSDSFGINHDTIVQTVGEIAEVVK
                     MGVQVGIVVGGGNIFRGVSAQAGSMDRATADYMGMMATVMNALALKDAFETLGIKARV
                     QSALSMQQIAETYARPKAIQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEMNCDVML
                     KATNVDGVYTADPKKDSSATRYETITFDEALLKNLKVMDATAFALCRERKLNIVVFGI
                     AKEGSLKRVVTGENEGTLVHC"
     gene            complement(15648..16502)
                     /gene="tsf"
                     /locus_tag="ACFM8S_000601"
     CDS             complement(15648..16502)
                     /gene="tsf"
                     /locus_tag="ACFM8S_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFC6313709.1"
                     /translation="MAEITAKMVADLRTATGLGMMECKKALVEAEGNFDKAEEILRIK
                     SGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCETDFVAKDAGFVEFANFVAKTAAE
                     KKPASVEELSELVEAERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
                     YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMV
                     EGRIRKFLAEITLNGQAFVMNPDQTVAQFAKENGTEVVSFVRYKVGDGIEKAVVDYAA
                     EVAAAAKV"
     gene            complement(16631..17359)
                     /gene="rpsB"
                     /locus_tag="ACFM8S_000602"
     CDS             complement(16631..17359)
                     /gene="rpsB"
                     /locus_tag="ACFM8S_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFC6313710.1"
                     /translation="MSQITMRQMIEAGVHFGHQTRFWNPKMAQYIFGARNKIHIVNLE
                     KTLPMFQDAQEAIRRLVANKGTVLFVGTKRQARDIIREEATRAGMPFVDYRWLGGMLT
                     NYKTVKQSIKRLEEKTAALENAAESGFSKKEILEMQRDVEKLERSLGGIKNMKGLPDA
                     IFVIDTGYQKGTLVEAEKLGIPVIAVVDTNNSPDGVKYVIPGNDDSAKAIRLYCRGIA
                     DAVLEGKNQALQETVAAAQEAAAE"
     gene            complement(17536..17769)
                     /locus_tag="ACFM8S_000603"
     CDS             complement(17536..17769)
                     /locus_tag="ACFM8S_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313711.1"
                     /translation="MWHIVAIGYLFVAVMYSAAQPSIARALIYLVFWAVLPTVFTVFV
                     VTVRRRNRLMKQQEQAEFEQQRAQRQKDSGTKP"
     gene            17847..18428
                     /locus_tag="ACFM8S_000604"
     CDS             17847..18428
                     /locus_tag="ACFM8S_000604"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFC6313712.1"
                     /translation="MRNEEKRALRRELRGRRSQMGRDVRAAATVKINRLLKRYIKHGR
                     KIGVYWPMGKELRLDGFVRAAQKRGAKLYLPYIEPRSRRMWFTPYPESGMERERIRGR
                     ARLNVPQFAGRKIRVHGLSVLLVPLVGIDREGYRLGQAGGYYDATLAAMKYRLQAKTV
                     GVGFACQLVDRLSVEAHDLPLDGFVSEAGILCF"
     gene            18489..19079
                     /locus_tag="ACFM8S_000605"
     CDS             18489..19079
                     /locus_tag="ACFM8S_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFC6313713.1"
                     /translation="MSEKIILPVLSLGGVRLEPLDVHHETGLREAVCDGEVWKLGVTS
                     APHPDRVADYIGTALATRLAFAVVDEEAGRVVGTTAYYHFEPQIPRLDIGFTWYAASA
                     RRTRINTCCKIMLLDYAFDVLACRCVGWRTDILNLASQRAIERLGAEKDGVLRMHMLR
                     KDGSVRDTVVYSMLREDWCKNREILTGRLAGYGVQV"
BASE COUNT         4861 a         4228 c         4278 g         5725 t
ORIGIN      
        1 acccgccagc cttcgggggt ttgtttgttg aggaacgcgc tgaaattgac ggggttgacc
       61 tttgccgcgc cgaaaaacag gctggacaac aggctttcct gataaatgac gactttgtat
      121 tctttcatag gattcctttt aacataaggg atcatctttt cagacgaccc cttaagttgc
      181 taatgatgta aaacagcaaa ccttattgct tattgcccaa ctgcggcaat accgaacctt
      241 cgcccagcga gagcaaaccg ctttgcgagt agatacccag tttgacgcgg gtgtcggtga
      301 tgtcgaggtt gcgcatcgtg agctgtccga tgcggtcgag cggagtgaac gcagcgtctt
      361 cgactttttc catactcagg cgttcaggtt ggtaggtcag gttgggcgat tcggtgttca
      421 gaattgagta gtcgttgccg cgccgcagtt cgagggtaac ttcgccggta acggctttag
      481 cgacccagcg ttgggcggtt tcgcgcaaca tcagggcttg gctgtcgaac cagcggcctt
      541 ggtagagcaa acgtccgagg cgcaggccgt tgatgcggta ttgttcgatg gtgtcctcgt
      601 tgtggatgcc ggttaccaag cgttcgtagg cgatgtggaa caacgccatt cccggggctt
      661 cgtagatgcc gcgcgatttg gcttcgatga tgcggttttc gatttggtcg ctcataccca
      721 gaccgtggcg gccgccgatg cggttggctt cgaggaagag ttcgacggga tcagcgtatt
      781 ctttgccgtt cagtgcaacc ggcacgcctt cttcaaagcg cacgctgact tcttcgggtt
      841 tgatttcgac gttttcgtcc cagaaggcaa cgtccataat aggtttgacg attttgatgc
      901 cgctgttgag gaattctaaa tctttggctt cgtgggtcgc gccgagcata ttggaatcgg
      961 tggagtaggc tttttcaacc gacattttgt agttgaagcc gttggcaatc agaaattcgc
     1021 tcatttcgtg gcggccgccg agttcgtcga taaattgctg gtcgagccag ggtttgtaga
     1081 ttttcagcgc gggattggtg agcaaaccgt agcggtagaa acgctcgatg tcgttgcctt
     1141 tgtaggtgct gccgtcgccc cagatgttta cgtcgtcttc tttcattgcg gaaacaagca
     1201 tcgtgccggt tacggcgcgg cccagaggcg tggtgttgaa ataggcgatg ccgccggtgg
     1261 aaacgtggaa cgcgccgcat tggatggcgg cgatgccttc gtgtgccaac tgcgcgcggc
     1321 agtcgattaa gcgggcgttt tccgcaccgt attccatcgc ttttttggga atggcgttgt
     1381 agtcgtcttc atcgggctga ccgaggttgg cagtgtaggc ataaggcagt gcgcctttga
     1441 gtttcatcca caacagcgcg gcggaggtat caagtccgcc ggagaaggcg atgccgactt
     1501 tctgaccgac ggggaggttt tgtaaaatag tatggttttg gttcatgaaa aaatcctcgg
     1561 tagcttgaaa aatcaaagcc attatcttat aaataggaaa tattgtcagc catcatgccg
     1621 tctgaacggg tattccgccg tttcaggcgg tatttaaaac cgaaaacatt atgaacgcgt
     1681 tcaaccttat catcatcggc gacgaaatct tacacggcag ccgccaagac aagcatttcg
     1741 tctttttcaa atccctgctc gaatccaaag ggctgaagct caatcaggtg caatacctgc
     1801 ccgacgaacc tgatttgttg gtcaaacaac tgcgccgcag cttttcagac ggcatgccaa
     1861 ctttcgttac cggcggtatc ggcgccacgc ccgacgacca cacccgccaa gccgcagccg
     1921 ctgctttgga tttgcccgtc gtccgccacg cgcaagccgc cgggtttatc gaaggcgtga
     1981 cccttaaacg cggtgagccg ctcgattcgc ccgaacacgc ccaacgcctg aagatggcgg
     2041 attttcccca aggcgcggaa ttggtgccta atccgtttaa taatatcgcc ggattttcca
     2101 ttcgcgagca ttatttcttc cctggttttc ctgtgatggc gcacccgatg gcggaatggg
     2161 tattggaaac ttattatgcc gaccgcttca accaaaccca acgcggcagc cgcagcgtgt
     2221 atgtgttcga gcagcccgaa tcgcgcatta cgccgcttat ggagcatatc gagcaaactt
     2281 atcccggcgt gcgttcgtac agcctgccca gcgttggttg gacgcattca gacggcacgc
     2341 aggtcaaacc gcacattgag ttcggcatca aagccgaagg agaagccgtc aaccttttgg
     2401 acgcggcgtg ggcggaagtg ttgcacagct tggacggatt gggagcagag ctgaaaaatc
     2461 gggtaaactg aaaaccgttt gcccaaatcg gacgatattt aaggaaaacc atgcaatacc
     2521 gaatcagaag agaaaacgaa gcacccgaag caaaaaatac gggggaaacc ttatgggagc
     2581 gcgacatcat gcgcgaagtg ctgctgtccg cctatcagga caggcgcagg gagcggatgt
     2641 ggaaaaacat ctggcgcgcc gtcagcaccc tgattctggt tgccctgatt gcaggcattt
     2701 tccgaaaaga cgaagccgca ttgcagttgg cgggcaacac gccgcatacc gcagtcgtca
     2761 atctgtacgg cgaaatcggc aacggtgtag aagaccaggt caaaaaactc aaagacggta
     2821 tggaggccgc ctacaaaaac ccgcaggcaa aagccatcgt catccgcgcc aacagccccg
     2881 gcggttcgcc cgtcgtctcc aacaccgctt ttgaagaaat acgccgtctg aaggcgcagc
     2941 accctggcat tcccgtttat ctcgtggcgg aagatatgtg cgcgtccggc tgctactaca
     3001 tcgcggcggc ggcggacaaa atctatgccg acccgtccag catcgtcggc agcatcggcg
     3061 ttatcggcag cagtttcgac gcgaccggcc tgatggaaaa aatcggcgtg aaacgcaggg
     3121 ttaaaattgc aggcagcaac aaaggcatgg gcgatccgtt ttcgcccgaa acgcccgaac
     3181 agtcaaaaat ctgggaagaa atgctgaccg gcatacacgg tgagttcatc aaagccgtca
     3241 aaaccggacg cggcggccgc ctcaaattcc gacaatatcc cgacgtgttc agcggtcgcg
     3301 tgtacacagg cgcggatgcg ctgaaagtcg ggctggtgga cggactcggc aatatttaca
     3361 gcgtggcgcg agacgtggtc aaagcccccg atgtggtgga ttacactccg aaggacgatt
     3421 tcggcagaat cttgggcaga cgcttcggcg cagaactcaa agcaagcgtg cgcgaagccc
     3481 tgcaggccgt ccggtagcgt tgtttacagt atttctaagg gaataccaaa ataaaaatat
     3541 acacaaaaac actcacctcc ccagtatgct gccactaagt cagccgcgtc actatttacc
     3601 gtgcacttaa agccgtaaaa ggcaggttgt cccgggaggt tttgttcatc ctgataccga
     3661 tcggttgttg ttgctcaaag gacagaaggc cgccgataaa cgagattacc tgtttgtcgc
     3721 tattgacgat ttttatactc tgccattttg ccggacaaaa ccgcagacag tgccgccaag
     3781 tttctgaccg aacatctggc tgacccctgc ttgtacctga ttgagtacgt ttactctgac
     3841 aatgataggt aatataaaga gccgtccaac atgctttcgg tgcagcttgt tataataatg
     3901 ggattggttg gaggcttgcc cgatttgctt gtccgcagac caacggtaag gcggagcggg
     3961 ttatccgtac cttgatggag atgtggcatg aggaacagtc gtttgacagt ccggaacatc
     4021 ggcaaaagga gttgtgctgc tttattaatt taggggctgc cctagatcac tagggaaatt
     4081 caaattaagt tagaattatc cctatgagag aaagtcgtct aagacggtat aaacaaaata
     4141 aactcatttt gttatcgtgg ctatgatgta ttggatgcgg gcgaatttag ccatttccgt
     4201 atcaatcaca gcacacattt tgccgaacga caaaaccata ttaatggaac tgagaacttt
     4261 tggaatcaag caaaacgtca tttacgcaag tttaacggca ttcccaaagc gcattttgag
     4321 ctgtatttaa aggagtgcga atggcgtttt aacaacagtg aaataaaagt tcttgttcca
     4381 ttttaaaaca attagtaaaa tcgagtttat cctagttgtc caaggcagcc tcatctcttt
     4441 atagatagct gcttaccaag tatccttaat tgctaattca agccaatctt caaaactatt
     4501 aaaaaattca tggttttcta tattatctaa attaatgaca ataaccctag gatttcctgt
     4561 tacatcatta ccatcaaaat aagcttccca ttctccatta ctagcgaatg ggataagatt
     4621 taaatcaggc aggatagaat ttctaaatat ataagctact tctgcccctt ctgttccata
     4681 attctcaaac caccagtgaa aattatgtgg ctgaatataa ggtattaaag atgtatcgtt
     4741 agagatacga acatactttt caggataaca aaacctttca ggaagcatat tactaggata
     4801 taaattaaat tcattttgaa taatcatttc gttaccttag gatcataaat actaccatta
     4861 ggaaactggg tagaccaatt tggtggtgct agttcatttt caatttgttt tttaattcta
     4921 aattgaatag cttctcccca tgtttgcggc atctaatgct gcttgctctg catcaatatt
     4981 actaaaaatt ggattagtct caaaaatgaa aaattactgt tttttcattt tttatagcat
     5041 attcaagtgc atgaataata tttttcacta agggctctgt agaaaaatct gaattttgta
     5101 atttttcttt tacagccaaa atactttcct tatctattag ccaatccccc tcacttggtc
     5161 caaataacca attatcggta atttcagaaa caagccccca taatccacga ttttgatatt
     5221 caataatttg attttccgta atttctacaa ctgtttcaga ttgaatgttt ttataagccc
     5281 aatcaatcat ttcagtatat tcactaattg aaacattgtc ttttaaacag aaaattaaca
     5341 ggttgtgttc attcatttgt ctaagttcct gatatgagtt tgttgtaaat aatcttttgc
     5401 ttgcttacct tgtaaattaa aggattaacc gcaccttgga tgatgccgcg aatatcatcc
     5461 atacggttac cccaccaatt tttattggtg tatcgttaat catttatttc aaaaaagtta
     5521 tttattatca tattatcagg gagcaggtcg ttaaaaaaaa ccccctcaaa ttccattaaa
     5581 ttagtattta tccagcacat aaacgaacca aagcgttctt tccctgagtg taaatattta
     5641 aaattaaact caaaataagt ttttttgctt agaaaattta atggtttttc tatagtgaaa
     5701 aaaagccctt actcgttttc gttcgagata ctattttaaa taaattttta ttttcaataa
     5761 aataagaata ttcatttact aagttgttat gataaaaaag taattttatt aaaatgtttt
     5821 cataattttc taggttcatt tctagctcaa tttaaaatga tttcattgtt gccgtaccaa
     5881 tgcgaaattt accatcaaca aaatttccct tcactgatac aggtacacca tcaacttggg
     5941 tagttttatt ttcaggctgc ctgaaagaaa gataggcaac ttattactca tcttatgcta
     6001 gccctcaata attgaatacg ttgcactaga accaattatt tctatgaatg tttgctcaaa
     6061 cataaaaata tagtatttaa agaattctct attcaaagga aaataacttt ctttcaatat
     6121 ccattccgca taacactcat caagaagttc aaaaaaagat tgaccaccat tttcatgaag
     6181 caaagctcta tctgcatctt ccgaaattaa atccataaaa gtatctataa aatacgattc
     6241 atcaacaacc ctaaaatgaa taacatcttc aataaaaatt tttatggttt tatccgtatt
     6301 tctataacca actgctataa cttctacttt atcagagttt attgctgcat tatcaaaata
     6361 gaaatagcca gttgggaaat ctaagctttt taatttcata atttatcgtg tatttccata
     6421 tctgattttt atttttcggt tattactaat tgttatttca agtgttggtt ctccaccact
     6481 actatcatta cgagcattaa ccgcacgcct atcaggtaaa ttgcccgtta tcgttccatt
     6541 aggacgtgtt tgtacatttc gaacattcag actattaaaa tcatttagtg cgtcattgta
     6601 atcccccact ttatcatact gaacagcggg accttttgtt ttactccttt gagtagtatt
     6661 gctcaaaatg tctttgattt gtaaatcact gcgcacctta ccggtttcta tgcgagctgc
     6721 cacactactc gggctatagg ggtcgctatc aatcgcagga ttaccaccgc tggttttttg
     6781 tccgctagcc tgtttcccaa cctgctgtac tgtatcgcca atttcatcta ccgtacctaa
     6841 cgccttagtc tgcatatggc gaacagcagg ttttttatag ccggtatttt taacccaatc
     6901 ccatttagtc gggttaagtt ctactttttt acctctccaa accgtacctg cggcctctgc
     6961 tacggaaagg gcagtttggg ctgttgcagt tatattcgga tgggcatcag cccattgtct
     7021 ggcagagttg atactgtctt ttaccgcaaa agcactgtct tgtaatgcgg ttgcggccgc
     7081 aagttgtgct tcgggactta aatttcctaa attattaata ccttgtagag tctgctgcgc
     7141 agagatgtag caactgtatt tttaccccgt cgggtaaaaa tacagttgct acggctgctg
     7201 ggaaagcagt tactattttc ccagtggctc aaaattaatt gtttcagcag atatttttat
     7261 aataatccct acatcaataa taatttcact ataaaattgg tatttatata gctctatttc
     7321 gggtataaac aagggaaaca agctatcttc cttatcctca taatctacga aattatttgt
     7381 atctaattta aaattcaaaa tattactgca tttgatatat ccgaaataat taccttcaat
     7441 tgtatctgta aaagataatg taagggtatt tccattatcg gaaaagatta tttcataaag
     7501 gcttacattg tgaaaaatat tttttttcat ttgacaaaat atccagaatg atgccgatta
     7561 tttggcaatg gattgccttc ataatcttgt cgttgcggat attttccctg tacaccatcg
     7621 gttcgcggca ctctttgggt tcttataacc tctccatttg gacgaatatc tattttcacg
     7681 ccatcaggac ctttccatgt ttcatattga gcctcatctg aaccagtacg tctaaaacct
     7741 tgttcttgca atgcttgacg taattcatct cgcgtagttg gtctgataga tttaacaact
     7801 ggcttgtttc ctcctgccat ctcaccgtcc aacgtctgca taggtcgggc agcaggtttt
     7861 ttatagccgg tatttttaac ccaatcccat ttggtcgggt taaggtttac ttttttaccg
     7921 ccccaaaccg tacctgcggc ctctgctatg gcaagagcag tttgggctgt tgcagttata
     7981 ttcggatggg catcagccca ttgtctggcg gaattaatgc cgtcttttac cgcaaaagca
     8041 ctgtcctgta ataggctcgc ggcagcaagt tgtgcttccg gacttaaatt tcctaattca
     8101 ttaatatctt gtaaagtttg ctgcgcagag atgtaggtcg gatatttgta tccgacaaaa
     8161 atatttgacg catctatcgt ttccgaaatg ccgctgttgg aaaatgtcgg attcgagaat
     8221 ccgacctacg gctgctacca accatcattt tcagcttctt ttagccaagt atcaaaattt
     8281 tcaaaagtct cgtaatattt tgtattatct aaattaacta ctataatttt aggattacct
     8341 gatttatctg aaatatcaaa acaggcagcc cactcttggt ttctagcaaa aggtaatagg
     8401 tttggaatgc cagttatttc aaaataaatg tcaattactt ctgatatatt gctttctgca
     8461 ttttcaaacc accaaggaaa agaatattgt tcatcgtagt taatggcatg agtggattga
     8521 gctaatttta aataactttg cggatattta aatcctttag gaagcattgc ttcaggatac
     8581 ggaacaaaac tattttgttt tatcatttcc taagtacctt tacatcataa atacttccgt
     8641 taggaaatct tttgctccac ccttctggaa ctcctttttg tgtttcttgc tttctaattc
     8701 gtagctggat agcttctccc caagtttgag gctcaatacc atcatgctta aacctgattc
     8761 tctcttttga tgataatcgg tcattaaaat tatgaacatt cgcttccact tttactcctg
     8821 cttcacgtgc tgccataata cgcgtgttat ccatactagt aggtgctcca tcaggcatat
     8881 taacaacatc aaccctgtcg ccaacccatg atttttttct ttcatttcgg ctacaataga
     8941 atcatagttg taatttgcac taggctctga caaaaatttg ggttgttaag ttgtcatttt
     9001 tttataaatt ccagcaaact gttgttgtaa attatctact tcatgttttc taacaccaga
     9061 tacattttca aattctcttg aatctacaca tacgcccgca tatatctcat tcaagatagc
     9121 tcgtaaaaca aaaacttcat cttgagttag tgaaatagtt gagttttgat tatttatgga
     9181 tagtatattc atggcttttt cctataaatc atcataatat tttttacctg atgttggtct
     9241 aaatgctgta cctccatcgt cagaattttt atctcggata actattgtcc ctgttttatc
     9301 atcccaatac gcagttctac cgcgaggtaa ctctttcata tttgttggat ggctaacaat
     9361 attttcaata tgccgagcaa aatctgctgg tgaattgata tttaaatccg taaattcttg
     9421 ttgtcttatg acatgcttgt tataagcatg cccactacta atttgttctc caattagttg
     9481 cgcttgtaaa gacggttgtg tggaagcatc tgctttgctg ttggacgtta tagatttagg
     9541 cggtctattc ccaccggcca tttccccatc caaagtatgc atggtgcgaa cagcaggtgt
     9601 tttatagccg gtatttttaa cccaatccca tttggtcggg ttaagttcta cttttttacc
     9661 gccccaaacc gtagttgcgg cctctgctac ggcaagggca gtttgggctg ttgctgttat
     9721 attcggatgg gcatcagccc attgtctggc ggaattaatg ccgtctttta ccgcaaaggc
     9781 actgtcctgt aataggctcg cggcagcaag ttgtgcttcc ggacttaatt ttcctaaatc
     9841 attaatacct tgtagagtct gctgagcggc tgtatctgtg acagggctta ccgcactgtc
     9901 tgtaattgcc ccaatcccta ctccttgaaa acccattaaa aaaggattaa ccgcaccttg
     9961 gacgatgccg cgaatatcat ccatacggtt agcccaccaa tttttattgg ggtcgttttc
    10021 ccatatcagt tttcctgctt catcagcaca gttaggattg gcattgatgc ccacccagtt
    10081 tcaggcagcc tgaaaaggat ttttaatata cggcaaaagg tgggcaacaa gtgcccacct
    10141 tacctagcta acatttatcc caagtatcat atattttttt aagttcttta tccaaagaaa
    10201 gaactttatt ttttcaacac catgaattat ctcaaaatct ctacaatcca cgcatacgcc
    10261 tgaataaatt tctcctacaa ttgcttgaat aatatacaac tcatctttat cgagactaat
    10321 tttaactgaa ctctcttcaa aattaattaa tttcatgaat aattcctttt attttaattc
    10381 gtcatcaaaa taccgtttgc ctgtttttgg tctaaatgct gtccaccatc tttagtcttg
    10441 gatatttaga ttataatcgt caaattccca gtcaaaatat tttgccaaat ttgatataga
    10501 cttatcttca gacgttgaat tacatatttc caatatatca tttatcaatt ttaatatgtg
    10561 ttcaatatta aaacggtttg taacaattga gttttttaat gacatcggtc tatgtatatt
    10621 tgaaagtaac cattcagtgc tatatatata aacataaaaa taatcagcac cattactttc
    10681 atcaaaacct attgataatg tcaaaagtat acaaaaattt tctaacttgt cttgtgggaa
    10741 atcttccaaa tcaaagtcag aacaatcata attttttaat tttaatctca tataattacc
    10801 taatatttaa atgtccattt ttaattttat tcctttttcc atttgaatca atagtataag
    10861 tttcaaaatt tgcttgaata cctgtagttg caaattgtga atttttttct gttggtggcc
    10921 gatattgtcg agtgccatct atacttaacc atccgcctcc actagtttgt cttgcaccct
    10981 caccaaccca aattttacct agcctatctg cctcttcata agttgcagtt cctattggaa
    11041 aatttttttc actcttatga agagctagac tcaatcttgg atcttgagcc gcaatgtttt
    11101 ttaagttttg cccagttaat tgattaacta attgaggata agttgcagca ttggcttttc
    11161 ctccagacgt tatagattta ggcggtttat tcccaccggc catttcccca tctaaagtct
    11221 gcataggtcg ggcagcaggt gttttataat cggtattttt aacccaatcc catttagtcg
    11281 ggttaagttc tactttttta ccgccccaaa ccgtacctgc ggcctctgct atggcaaggg
    11341 cagtttgggc tgttgcagtt atattcggat gggcatcagc ccattgtctg gcagagttga
    11401 tactgtcttt taccgcaaaa gcactgtcct gtaataggct cacggcggca agttgtgctt
    11461 ccggacttaa atttcctaaa tcattaatac cttgtagagt ctgctgcgca gagatgtagg
    11521 tcggatattt gtatccgaca aaaatatttg acgcatctat cgtttccgaa atgccgctgt
    11581 tggaaaatgt cggattcgag aatctgacct acggctgctg actattttct tgtttatata
    11641 attctacaac tgcaaactac tatcaaatct ttttatctct ggctctacac tcagcaagaa
    11701 tatttttatt ttacaaaaca taggctagtt atttgagtat attgtcaaat tctaatttgt
    11761 ttttttgata gatttttttt ataccattaa tacattcatt agttgtaatt ctaccaagcg
    11821 aatgtaattt atcaatatca aaacaatcgg tataaaccct attcacagtt ttatcttgta
    11881 tttccttatg aatacttaat atattgagat agtttagaaa ttttattttc tcagattgag
    11941 gtaactcatc aaaaccaagc caataataaa aattgttata tgattttaaa aaatcaacaa
    12001 tttttatttt tcctaaaacc aaatcttcaa ctaactgggt tgcataaata atatcattta
    12061 tgattttaac aggtaaatta tttgatgggt aaccatgggg ttccgtcatc atttgtaact
    12121 cctggtacat gtgcgtgtcc tgtaagtgcg cgtgggtctt gatgggtatt tgcatgatga
    12181 gcatcatcta ttctggctcc agtcaagttt ccattactat ctcggacttg gataaaacga
    12241 gcatcaggcg aacctgttat ataaccgcct attggtgttg gtattgcatt tcctttgctg
    12301 tctacataaa ttgttcctgt cggagaaata gatgtatcag gtttaacata tggggcaggt
    12361 aagcctatat tgttatcaga gtgtgtagag tgttgctgcg tacttggttt cggtggctta
    12421 tttcccccag ccatctcccc gtctacagtc tgcataaggc gaacagcagg ttttttatag
    12481 ccgttatttt taacccaatc ccatttggtc gggttaagtt ctactttttt acctctccaa
    12541 accgtacctg cagcctctgc tacggaaagg gcagtttggg cagtagctgt aatgttcgga
    12601 tgggcatcag cccattgtct ggcagagttg atactgtctt ttaccgcaaa agcactgtct
    12661 tgtaatgcgg ttgcggccgc aagttgtgct tccggactta aatttcctaa attgtgaata
    12721 ccctgtaaag ttttccgtgc tgccgcatag gttaccgggt ttaccgcact gtctgtaatt
    12781 gccccaactc ccaatccctg aaaacccgtt agaaaaggat tggctgcacc ttggatgatg
    12841 ccgcgaatat catccatacg gttaccccac caatttttat tggggtcgct ttcccatatc
    12901 agttttcctg cttcatccgc acggctgaaa aaaccagagg cggcaccggc attttctttt
    12961 agccaacggt ctgaaaatgg ggcttgaggg acaatattag tctttgtttt tgttgaagtt
    13021 cctttgacat agtagctgta tatatccctt gctcctccgg ggggcggata atcgctgcct
    13081 tgcggtccgt catatccatc ctccggatgg atttccgacc ctgtccgatg aagttggtaa
    13141 acagtaaaac cgccgtctac accgccgctg aaatcagaag tgctttttga atcatgatga
    13201 tcgaacggac tgtgtacttc atgtccgtgc cctgaaaaat gggtttcata gccgataaca
    13261 ccttcaaacc ctgtccgttc gtgtgtaata ggcagtatgg ggccgaccgc agttgcatca
    13321 aatgtttgga cggcgtaaac ccgattttta acactgccgc gaccatcacc gaacagatgg
    13381 tatttgccac ccggttcgta gtgttttgcc tgcatatcat cgcgcaaacg ggcatccaaa
    13441 ccgttcgcat gactaatggg gatttgcagc aacgatattg ctgctgcaaa cagcatcatg
    13501 aatttttgaa taggcaaatt cattttgttt tccttggtag caagcggtta tggtagtgtg
    13561 aatcaaggtt gcccttgtct atgttgtcgc actacttcat cgctgtatcc atacccctca
    13621 tgactgttat cagcctctac ggatggggca gagttacccg tatgattgcc gtatggtcgg
    13681 atatcggaga aatcgaccat taatccttcc gtcggtttaa ttcctttgct tactttatac
    13741 ggccccatcc acaatgcgta attttcttta taggcagctt caaacgcatt ggttttgggt
    13801 ttgatgagca attttttatt ggttctgtct actgcgaaat attccagttt tgtttgggct
    13861 ttcagtgttt cggcattgta taggtgcatt tcggttctgt tgcgtatcgt tccgaatacg
    13921 tcgatgttaa taaacacatc tgtatcggca ttggcaggag aaacaacgtc tatgccgcgc
    13981 aggaaaaata cggtctgtac caagtgggaa agaaaggcag tgtcgcgcgg gttagtcgtc
    14041 aaggtttcat ttcgataatc ccccatcccg ccaatattta agcccagact gcttttactt
    14101 ccgctaccgt ctgattgggt gcgagagcgt gcaggggcat taagtgtaga taaagaagtg
    14161 gttaaacctg tcaaaccgcc tgatgttgtt tcagcggtgg tttcgtaacg tggataggtg
    14221 taatcggtac ggacggcagg gctgtttatg tattcgccac gaatcagtgc atcaatggag
    14281 tagcgacccc ctgtcaaact gcctgaacct tggtcgccca tagttgcaat gtacaatgca
    14341 acttttcgtc cgtgtaatgc ctgtaaatcc atgtctttaa cggcagctct ggcagaagcg
    14401 gccacaagtt cttgttcgac cgcaaagcgt ttacctccgc catgcgatgg aatacctgtc
    14461 agtgtcccgc aggcggataa aataaaaact gaaaaaagaa taggtatcag cagccgtgct
    14521 tgcatagatt ttctcctttg atgaaaaaca aattgtatca aaattgtaaa tatagtggat
    14581 taaatttaaa ccagtacggc aaggcgaggc aacgccgtac tggtttaaat ttaatccact
    14641 ataatctcca tataataaat ggctgtctga agcccatatg cgactatatc tgccgacact
    14701 atggtcaatc agcagtgaac cagcgtacct tcgttttcac cggttactac acgtttgagc
    14761 gagccttctt tagcaatgcc gaatactaca atattgagct tgcgttcgcg gcagagggcg
    14821 aaagcggtgg catccatcac tttgaggttt ttcaacaagg cttcgtcaaa agtaatggtt
    14881 tcgtagcgcg tggcggatga gtcttttttg gggtctgcgg tgtacacgcc gtcaacgttg
    14941 gtggctttga gcatcacgtc gcagttcatt tccgcaccgc gcaatgcggc ggcagtgtcg
    15001 gtcgtgaaga acgggttacc ggtaccggcg gcaaaaatca cgactttgcc ttcttccaaa
    15061 tattggatgg ctttggggcg ggcgtaggtt tcagcgattt gctgcataga cagtgcggat
    15121 tgtacgcgcg ctttgatgcc taaagtttca aatgcgtctt tgagtgccaa cgcgttcatc
    15181 acggtcgcca tcatgcccat gtagtcggcg gtggcgcgat ccatgctgcc tgcttgggcg
    15241 gatacgcccc ggaaaatatt gccgccgccg acaacaatac cgacttgcac gcccatttta
    15301 acgacttcgg caatttcgcc gacagtttga acgatggtat cgtgattgat gccgaatgaa
    15361 tcggaaccca tcagggattc gccggagagt ttcagtaata cgcgtttgta tttgatttgc
    15421 tgtgtcatgg gataccttgc tttcttgaat gtcgtcggtc ggagtttggg ccgtctgaat
    15481 tttcaaacgg tcggaattac aatgatatgg tgtgtcgttc tattattgtt ttgttttcag
    15541 acggtctgaa tattcggtcg tcttaaaata ggtaccgcaa acggttttct caaaaaaaag
    15601 caccctgatt cgtttggaat ccaggtgctt tctttttcat aagtgcctta cactttggcg
    15661 gctgcggcta cttcggctgc gtagtcgaca acggctttct cgataccgtc gcctactttg
    15721 tagcgtacga agctaaccac ttcagtgccg ttttctttag cgaattgggc aacagtttgg
    15781 tcagggttca tcacgaatgc ttggccgttc agagtgattt cagccaagaa tttacggata
    15841 cggccttcaa ccattttagc ggcgatgtcg gcaggtttgc cggaagcgat ggcttgttcg
    15901 gtgtagatgt ggcgttcttt ttcaacggtt tcagcatcta cttcagcttc gcttacgcat
    15961 tgtggtttag cggcaacgat gtgcataccg attttgcgtg ctacgtcttc agagcctttg
    16021 tactcaacca atacgccttc ggtagccaat gcaccgtgga tgtaggcaac cagttggttg
    16081 gcagtgtcga tcacttggaa gcggcgaaca gacatgttct cgcccaattt agcgatgatg
    16141 gctttacgtt ctgcttcaac cagttcgctc agttcttcaa cagaagccgg ttttttctcg
    16201 gcggcagttt tcgcaacgaa gttggcaaat tctacgaagc ccgcgtcttt agcaacgaag
    16261 tcggtttcgc agtttacttc gaccaatgcg ccgacattgc cgttgatcgc gtaagccaat
    16321 acgccttcgg cagcagtacg gccagccagt ttaccggctt tcgcaccaga tttaatacgc
    16381 aggatttctt cggctttgtc gaagttgcct tcggcttcaa ccaaggcttt tttgcattcc
    16441 atcatgccca ggccggtagc agtgcgcagg tcggcaacca tttttgcagt aatttctgcc
    16501 attttgaatc tcctagattt ttgggaaata cggaaaggat tttccgcatt cgggaatcgt
    16561 gtgccgcgaa cggacgttca gacggcatat ttgagaaaag gggcataacg cccctcttcg
    16621 gtttgccgga ttactcggca gcggcttctt gggcagcggc tacggtttct tgcagcgctt
    16681 ggtttttgcc ttccaaaact gcgtcagcga tgccgcggca gtacaggcgg atggctttgg
    16741 cggagtcgtc gttgccgggg atgacgtatt tcacgccgtc agggctgttg ttggtatcga
    16801 ctacggcgat aacggggatg cccagttttt cagcctcgac cagagtacct ttttggtagc
    16861 cggtatcgat aacgaaaatc gcgtcgggca ggcctttcat gtttttgata ccgcccaaag
    16921 aacgttccag tttttcaaca tcgcgttgca tttccagaat ttcttttttg ctgaaaccgc
    16981 tttcggcagc attttccaag gctgcggttt tttcttccag gcgtttgatg gattgcttaa
    17041 cggttttgta gttggtcagc ataccgccca accagcggta atcgacgaaa ggcataccgg
    17101 cgcgggtcgc ttcttcgcgg atgatgtcgc gtgcctggcg tttggtacct acgaacaata
    17161 ctgtaccttt gttggcaacc agacggcgta tggcttcttg cgcgtcttgg aacatcggca
    17221 gggttttttc caggttgacg atatggattt tgttgcgcgc accgaaaatg tattgtgcca
    17281 ttttcgggtt ccagaaacgg gtttggtggc cgaagtggac accggcttca atcatctgac
    17341 gcatagtaat ttgagacatt ttgatttcct tgaaagggtt aagggcacac attcaatcgc
    17401 atagaacttg ataggcgacg cctattgtca agcacccttc ggcgaaagtg ggcataagtt
    17461 ttaaaaaata aaagagtgtt tccgaaatgg aaaactgacg gattatagcg gataagaggc
    17521 cgtctgaaaa gggattcagg gttttgtgcc gctgtctttt tgccgttgtg cacgctgctg
    17581 ttcgaattct gcctgttcct gttgcttcat caagcggttg cggcggcgga cggttacgac
    17641 gaagaccgtg aacacggtgg gcagcaccgc ccaaaaaacc aaataaatca aggcgcgcgc
    17701 aatactcggc tgcgcggcgg aatacataac ggcaacaaaa agatagccga tggcgacgat
    17761 gtgccacatt gtgtgcgtcc tgtttgaatg ttaacaaaat cgttataatc gcaacattct
    17821 accgcaatcc tgaaaggccg tctgaaatga ggaatgagga aaaacgcgcc ctgcgccgcg
    17881 aattgcgcgg gcggcgttcg caaatggggc gggacgtgcg ggcggcggca acggtaaaaa
    17941 ttaaccgcct gctcaaacgt tatatcaagc acggtcggaa aatcggcgtg tattggccga
    18001 tgggcaagga attgcgtttg gacggctttg tccgcgcggc gcaaaaacgc ggcgcaaaac
    18061 tttatctgcc ttatatcgaa ccgcgttcgc ggcggatgtg gtttacgccg tatcctgaaa
    18121 gcggaatgga acgggagcgc atacggggca gggcgaggtt gaacgtgccg cagtttgcag
    18181 ggcgcaaaat ccgcgtgcac ggtttgtcgg tattgctcgt tccgcttgtc ggcatagacc
    18241 gcgaaggcta ccgtttgggg caggcaggcg gctattacga tgcgacgctt gcagcgatga
    18301 aataccgttt gcaggcaaaa accgtgggcg tgggctttgc ctgccagttg gtggacagac
    18361 tgtcggtcga ggcgcacgac ctgccgctgg acggttttgt gtcggaggcg gggatattgt
    18421 gtttttagcc gttcagacgg cattttgaac ggaaatgccg tctgaaggtg gaatattggg
    18481 agaaaaggat gtcggaaaag ataatactgc cggtcttgtc gttgggtggc gtacggctcg
    18541 aaccgttgga tgtgcatcat gaaacgggtt tgcgcgaggc ggtttgcgat ggggaagttt
    18601 ggaagctggg agtaacgtcc gcgccccatc cggatagggt ggcagactat atcgggacag
    18661 cattggcaac gcgtttggcg tttgcggttg tcgatgaaga ggcgggcagg gtggtcggga
    18721 caacggcgta ttatcatttt gaaccgcaga ttccgcgttt ggatattgga ttcacatggt
    18781 atgcggcatc ggcacggcgg acacggataa acacctgttg caaaatcatg ctgttggatt
    18841 acgcatttga tgttttggct tgccgttgcg tgggatggcg cacggatatt ctgaaccttg
    18901 cctcgcaacg tgccatcgaa cggctgggtg cggaaaaaga cggcgtgctg cgtatgcata
    18961 tgctccgtaa agacggcagt gtgcgcgata cggtcgtgta cagtatgctg agggaagatt
    19021 ggtgcaaaaa cagggaaatc ctgaccggca ggttggcagg gtatggcgtg caggtgtgag
    19081 gcagtatttt ca
//
LOCUS       DAXHSF010000021        18476 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.021, whole genome shotgun sequence.
ACCESSION   DAXHSF010000021 DAXHSF010000000
VERSION     DAXHSF010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18476
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.021"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            79..4555
                     /locus_tag="ACFM8S_000606"
                     /pseudo
     CDS             79..4555
                     /locus_tag="ACFM8S_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
     gene            4787..6241
                     /gene="mgtE"
                     /locus_tag="ACFM8S_000607"
     CDS             4787..6241
                     /gene="mgtE"
                     /locus_tag="ACFM8S_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFC6313714.1"
                     /translation="MSIEPTPPNLENDGIENDVERVSADFDRVHSLCEILEPAFEQIE
                     NGTPLEDAPLRDKLTELTVLLSELHPADVAAVLESLPPRERNIVWILVKPEDDGEVLL
                     EVSDAVRETLIESMDKDELLAAVDDLDADELAELADDLPHQVVYEALQTRDEEERAQV
                     KAAMSYEDNQVGAIMDFELVSIRADVACEVVLRYLRRFDSLPDHTDKIFVVDENDVLQ
                     GVLPIRKLLVADPEDLVENVMAKDVVRFRAEDDVEEAAQAFERYDLVTAPVVDENKKL
                     IGRVTVDEMVDVIREESEADMLNMAGLQEEEDLFAPVLDSVKNRWMWLAVNLCTAFLA
                     SRVIGAFEGSIEKIVALAALMPIVAGIGGNSGNQTITMIVRAMAMGQLTDMQAGRLLK
                     KEVGVALVNGIIWGTVMGAVSWLLYGSLGIGLVMVAAMTLNLLLAATVGVLIPVVMEK
                     FGRDPALGSSVLITAVTDSGGFLIFLGLATLFLL"
     gene            complement(6327..7235)
                     /gene="hslO"
                     /locus_tag="ACFM8S_000608"
     CDS             complement(6327..7235)
                     /gene="hslO"
                     /locus_tag="ACFM8S_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFC6313715.1"
                     /translation="MNQTAINRADVRTRFIFDDMPVRGLHVRLENVWQHIVKQKNYPA
                     AIRRALGELLAAGVLLSGNLKNEGTLIVQVQGRGRLKMLVAEAASDRTVRATARWDET
                     AEIADDESLGDLLGEGGVFVLTLQPKDGEPWQGVVPLEGGGIAQMLANYMKRSEQIDT
                     HIVLSASDEAAGGLLVQRLPEAVLDEEAWEHVSTLARTLTADELAELDAQHVLYRLFH
                     ETPPRVFEPETFEFSCTCSRGKVSDMLLMLGGEEVGGVVVEQGSIEVDCDFCHSKYVF
                     DETDVNALFGEDVVGVAKGLPRHTVQ"
     gene            7480..8235
                     /locus_tag="ACFM8S_000609"
     CDS             7480..8235
                     /locus_tag="ACFM8S_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFC6313716.1"
                     /translation="MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAED
                     EQPVLPINRAPARRAGNADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLN
                     EQPVLPVNRAPARRAGNADELIGNAMGLLGIAYRYGGTSVSTGFDCSGFMQHIFKRAM
                     GINLPRTSAEQARMGTPVARSELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTG
                     KNIEITSLSHKYWSGKYAFARRVKKNDPSRFLN"
     gene            8254..8472
                     /locus_tag="ACFM8S_000610"
     CDS             8254..8472
                     /locus_tag="ACFM8S_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313717.1"
                     /translation="MSMPEMPKWYDDDGQIVSCTEKVKVMSENMAELYQTAQDAFEDA
                     LLMGCGERQLRDYLLALIEGLENPYRKV"
     gene            8617..8961
                     /locus_tag="ACFM8S_000611"
     CDS             8617..8961
                     /locus_tag="ACFM8S_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFC6313718.1"
                     /translation="MNIIRALLIILGCLATGETAVFLAGIKLPGSIVGMGVLFALLQA
                     GWVKTSWLQQLTDALMANLTLFLVPPCVAVISYLDLIADDWFSILVSASASTLCVLLV
                     TGKVHRWIRSII"
     gene            8961..9653
                     /locus_tag="ACFM8S_000612"
     CDS             8961..9653
                     /locus_tag="ACFM8S_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFC6313719.1"
                     /translation="MNEILRQPSVLLFLTLAVYALAIIVRTRTGNIFCNPVLVSTIVL
                     IAYLKILDIDYAVYHNAAQFIDFWLKPAVVVLAVPLYQNRRKIFNQWLPVIVSQLAGS
                     VTGIVTGMYFAKWLGAEREVVLSLASKSVTNPIAIEITRSIGGIPAITAATVIIAGLV
                     GQIAGYKMLKNTVVMPSSVGMSLGTASHAMGIAASLERSRRMAAYAGLGLTFNGVLTA
                     LIAPLLIPVLGF"
     gene            9720..10940
                     /gene="argJ"
                     /locus_tag="ACFM8S_000613"
     CDS             9720..10940
                     /gene="argJ"
                     /locus_tag="ACFM8S_000613"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HFC6313720.1"
                     /translation="MAVNLTEKTAEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAG
                     STVGAVFTTNRFCAAPVHIAKSHLFNEDGVRALIINTGNANAGTGAQGRIDALAVCAA
                     AARQIGCKPNQVLPFSTGVILEPLPADKIIAALPKMQPAFWNEAARAIMTTDTVPKAA
                     SREGKVGDKHTVRATGIAKGSGMIHPNMATMLGFIATDAKVSQPVLQLMTQEIADETF
                     NTITVDGDTSTNDSFVIIATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDG
                     EGATKFITVRVENAKTRDEARQAAYAVARSPLVKTAFFASDPNLGRLLAAIGYAGVVD
                     LDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKAEITVRIKLHRGQSAATVYTCD
                     LSHGYVSINADYRS"
     gene            11000..12409
                     /locus_tag="ACFM8S_000614"
     CDS             11000..12409
                     /locus_tag="ACFM8S_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFC6313721.1"
                     /translation="MTSTFPRRLARKIRQTRRLSRKSIAFLFLLAGSALVALTALFFA
                     HLADFALELNAKLVQQYPWFAWVALPLGLPLIAWLTRKFAPFTAGSGIPQVIASLSLP
                     YGAQKTRLIRLGQTLLKIPLTFLGMLFGASIGREGPSVQVGAAVMGAWGAWCKKHGLA
                     FKGMQENDLMAAGAAGGLAAAFNAPLAGVIFAIEELGRGIMLRWERQILLGVLASGFI
                     QVAIQGNNPYFSGFNGGVLEHIFLWVALSGLVCGAAGGLFGRLLYRGAAAFAPRKIRG
                     FIRNRPLLLAALMGLLLALLGTFYQGKTYGTGYHEAAQALHGIYEAPFGLAAAKWLAT
                     VFSYWAGIPGGIFTPSLTIGAVLGEHIAAIADISQGANIIVLICMAAFLAGATQSPIT
                     SAVVVMEMTGGQSLLFWMLIACIFASQVSRQFSPRPFYHASGMRFRQRVLQETAAQTG
                     NAPARPQTANSKTGMPSEN"
     gene            12599..13990
                     /gene="hrpA"
                     /locus_tag="ACFM8S_000615"
     CDS             12599..13990
                     /gene="hrpA"
                     /locus_tag="ACFM8S_000615"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFC6313722.1"
                     /translation="MFPDFSQTLSKDRHFLRSAFKNPNKYGGLSKVEEKYQKSHEIFL
                     KRLAALPKPEFDNTLPVHEKLDEIKKAIAENQVTIICGETGSGKTTQLPKICLELGRG
                     AAGLIGHTQPRRLAARSVAERIAEELKSEIGSAVGYKVRFTDHTSRDACVKLMTDGIL
                     LAETQTDRYLAAYDTIIIDEAHERSLNIDFLLGYLKQLLPRRPDLKVIITSATIDAER
                     FSQHFNGAPVLEVSGRTYPVEILYRPLIGKDEDDAEIELTDAIVDAADELARYGEGDI
                     LVFLPGEREIREAAEALRKSTLRRNDEILPLFARLSHAEQHKIFHPSGAKRRIVLATN
                     VAETSLTVPGIKYVIDTGLARVKRYSARAKVEQLHVEKISQAAARQRSGRCGRVSAGV
                     CIRLFSEEDFNSRPEFTDPEIVRSNLAAVILRMASLKLGDVAAFPFLEMPDSRYINDG
                     FQVLLELGAVEAA"
     gene            14091..15218
                     /locus_tag="ACFM8S_000616"
     CDS             14091..15218
                     /locus_tag="ACFM8S_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HFC6313723.1"
                     /translation="MKHLLIDFENVQPQNLDKLLTENTHIWLFIGVLHKMLPISLVQS
                     LLRFGERVHLVQLQKTGKNALDFYLSYYLGQITATDPNAQIGILSRDGGYDVLVEHIL
                     KNHQAKGIVRLANIDEVQHQKIATEPPSALRENTPQPETTLKPQQPLTSYFQAALTAL
                     RRPDAFRPCRLHNLRQNLRKHILSDLFKEKTDEECEITTANVINKLKAQNFISIDEQE
                     TVSYHLSDNDLLQRIQRHILSQRPKTYADFQAVVQNRADALHLTVGTNDIQSFARHLR
                     DQNLIRQNNGKIEYAPFTEPKPQPTPKQPKKTAWEPDEIIWKKVIAALSLKNRPNKTK
                     TLRNTIQALTKSNAQETDKLLQHLQDTQVLRIDGTKIVYIK"
     gene            15395..16972
                     /gene="lpt3"
                     /locus_tag="ACFM8S_000617"
     CDS             15395..16972
                     /gene="lpt3"
                     /locus_tag="ACFM8S_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase"
                     /protein_id="HFC6313724.1"
                     /translation="MMKKSLFVLFLYSSLLTASEIAYRFVFGIETLPAAKMAETFALT
                     FMIAALYLFARYKASRLLIAVFFAFSIIANNVHYAVYQSWMTGINYWLMLKEITEVGG
                     AGASMLDKLWLPALWGMLEVMLFCSLAKFRRKTHFSADILFAFLMLMIFVRSFDTKQE
                     HGISPKPTYSRIKANYFSFGYFVGRVLPYQLFDLSKIPVFKQSAPSKIGQGSVQNIVL
                     IMGESESAAHLKLFGYGRETSPFLTQLSQADFKPIVKQSYSAGFMTAVSLPSFFNVIP
                     HANGLEQISGGDTNMFRLAKEQGYETYFYSAQAENQMAILNLIGKKWIDHLIQPTQLG
                     YGNGDNMPDEKLLPLFDKINLQQGRHFIVLHQRGSHAPYGALLQPQDKVFGEADIVDK
                     YDNTIHKTDQMIQTVFEQLQKQPDGNWLFAYTSDHGQYVRQDIYNQGTVQPDSYLVPL
                     VLYSPDKAVQQAANQAFAPCEIAFHQQLSTFLIHTLGYDMPVSGCREGSVTGNLITGD
                     AGSLSIRDGKAEYVYPQ"
     gene            17074..>18476
                     /locus_tag="ACFM8S_000618"
     CDS             17074..>18476
                     /locus_tag="ACFM8S_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HFC6313725.1"
                     /translation="MDARSNPANVSDGLQNSSGHIGTNTRYRLTKLGEQMARLPIDPK
                     IARILLAAKKHDCMAEILVIASALSIQDPRERPLEARDAAAKAHERFTDKQSDFLAYL
                     NIWDSFQRERDKGLSNKQLVQWCRQYFLSHLRMREWRELHHQLAQTAIEMGLTTKEAA
                     FRRPPEIRQLTSSENQGDQDLSAKLKQKQLDKKQHRAQIRAAKEAGYEQIHRALLTGL
                     IANVGMKSPDGNDYTGARGSRFHLFPASALFKAKPKWVMAAELVETTRLYARDVAAIQ
                     PEWIEQEAPHLVRYHYFEPHWEQKRGEVVAGERVTLYGLTVLPRRPVSYGKVAPEEAR
                     EIFIRSALVAQECDLKADFFVHNKKLIKEITELEHKSRKQDVLVDDEALFAFYHERLP
                     DFYTADAVSDGLRPANPQQTAPSYAREERREGKTVAAQTNFSATAANPLPNPLPQERE
                     QSAAASAVSNDLHPANP"
BASE COUNT         4574 a         5714 c         4528 g         3660 t
ORIGIN      
        1 ataaatccag cacaaaacgg gatcggtgat tcttgtccgc aagaatcgtt gattttctct
       61 attacacgga taatcatcat gcgcttcaca cataccatcc cattttattc cgtattgtcc
      121 accctcggtc tttttgccgt ttcccctgct tactcatcca ttgtccgcag cgatgtcgat
      181 taccaatatt ttcgcgactt tgccgaaaat aaaggcgcgt tcgccgtagg tgcaagcaat
      241 atttccatcc aagacaagca aggcaaaata ttaggcaggg ttctcagcgg catccccatg
      301 cccgacttcc gcgtcagcaa ccggcaaacc gctatcgcca ccctggttca cccccaatac
      361 gtcaacagtg tcaaacacaa cgtcggctac ggttccatac aattcggcaa cgacacccaa
      421 aaccctgaag aacaagccta tacctaccgc ctcgtatcac gcaacccgca cccggactac
      481 gactaccacc ttccccgcct caacaaactg gttaccgaaa tctcacctgc cgcactcagc
      541 agcgtaccct tgcttggaaa cggccagcca aaggccaatg cctacctcga taccgaccgc
      601 ttcccatact ttgtacgact cggctcaggc acgcaacaag tccgcaaagc agacggcacg
      661 cgtacacgaa ccgccccggc ataccaatac ctgaccggcg gcacgccgct gaaagtattg
      721 gggttccaaa accacggctt actcgtcggc ggcagcctga ccgaccaacc ccttaacacc
      781 tacgcagtcg ccggagacag cggttccccc ctgttcgcct tcgacaagca tgaaaaccgc
      841 tgggtgcttg cgggcgtact cggcacctac gccggcttcg acaacttctt caacaaatac
      901 atcgtcacgc aacccgaatt catccgttcc accatccgcc aatacgaaac ccggctggat
      961 gtcgggctga ccaccaacga actcatatgg cgcgacaacg gtaatggcgg cagcaccctg
     1021 caagggctca acgaacacat caccctgccc attgcaaacc cttcgcttgc cccgcaaaac
     1081 gacagcaggc acatgccgtc tgcagatgcc ggcaaaacgc tcatcctatc cagcaggttc
     1141 gacaacaaaa cactgatgct ggcagacaat atcaaccaag gcgcaggcgc attgcagttc
     1201 gacagcaact tcaccgtcgt cggtaaaaac cacacatggc aaggtgcagg cgttatcgta
     1261 gccgacggca aacgcgtctt ctggcaagtc agcaacccca aaggcgaccg gctctccaaa
     1321 ctgggcgcag gcacgcttat cgccaacgga caaggcatca accagggcga catcagcatc
     1381 ggggaaggca ctgtcgtact cgcccaaaaa gctgcttcag acggcagcaa acaagcattc
     1441 aaccaagtcg gcatcaccag cggcaggggc acggccgtcc tcgccgacag ccagcaaatc
     1501 aaacccgaaa acctctattt cggcttcagg ggcggacggc tcgacctcaa cggcaacaac
     1561 cttgccttta cccatatccg ccatgcggac ggcggcgcgc aaatcgtcaa tcacaaccct
     1621 gaccaagccg cgacactgac gctgaccggc aaccccatcc tcagtcccga gcatgtcgag
     1681 tgggtgcaat ggggcaaccg tccgcaaggc aacgcggcgg tttacgaata catcaacccg
     1741 caccgcaacc gtcggaccga ctacttcata ctcaaacccg gcggcaaccc gcgcgaattt
     1801 ttcccgttaa atatggaaaa ctcaacaagc tggcaattta tcggcaacaa caggcaacag
     1861 gccgccgaac aagtcgccca agccgaaaat gcccgccccg acctaattac cttcggcgga
     1921 tacttgggtg agaacgcgca aacgggcaaa gccgcaccga gttacagcaa aaccaatatc
     1981 gaatgggtga atggtccaaa caccccacac aagccctcgc cctatacgaa tacgccaacc
     2041 cgcacagggg caggcgtacc gacgattacc gcctcaaaga aagcggcaac cccggtgcct
     2101 acttcccatg gaatgcctac agcacccaag actgggaatt tctcggacat gaccggcgaa
     2161 gcagccatag aaaaaacccg ccatatcgca aatgccgccg tatacggccg gcccgaatac
     2221 cgttacaacg gcgcactcaa cctgcactat cgtcccaaac gcaccgacag cacgctgttg
     2281 ctcaacggcg gcatgaacct taacggggaa gtcttgattg agggcggcaa tatgattgtg
     2341 tcaggcaggc ccgtacccca tgcctacgac caccaggcca aacgcgaacc cgttcttgaa
     2401 aacgaatgga ccgacggcag cttcaaggct gcacggttca ccctgcgaaa ccatgcccga
     2461 ctgacggcag ggcgcaatac cgcgcatctg gacggcgaca taaccgcata cgatctgtcc
     2521 ggcatcgacc tcggctttat ccaaggcaaa acaccggaat gctaccgctc ctaccatagc
     2581 ggcagcaccc actgcacacc caacgccgtt ttaaaagccg aaaactatcg tgcactacct
     2641 gcaacgcaag tacgcggcga cattaccctt aacgaccgtt cagagctccg cctgggcaaa
     2701 gcacacctgt acggcagcat ccgtgccggc aaagacaccg cagtccgcat ggaagcagac
     2761 agcaactgga cactttccca gtccagccac accggcgcac tgacgcttga cggcgcacaa
     2821 attaccctga accccgattt cgccaataat acacacaaca accgcttcaa cacactgacc
     2881 gtcaacggca cacttgacgg gttcggcaca ttccgattcc tgaccggcat cgtccgaaaa
     2941 caaaatgccc ccccccctca aactggaagg ggacagccgc ggcgcattcc aaatccacgt
     3001 caaaaacacc ggacaagaac ctcaaacaac cgaatcgctt gcacttgtga gcctcaatcc
     3061 gaaacacagc caccaagccc gatttaccct ccaaaacggc tatgccgatt tgggtgccta
     3121 ccgctacatc ctccgcaaaa acaacaacgg atacagcctg tacaacccgc tcaaagaggc
     3181 cgaacttcaa attgaagcca cgcgtgcgga acatgagcgc aaccaacagg catacaacca
     3241 attacaggca accgacatca gcagacaggt tcaacatgac tctgacgcga ccaggcaggc
     3301 actacaggcc tggcagaaca gtcaaaccga acttgcccgc atcgacagcc aagtccaata
     3361 tctgtccgcc caattgaaac agacagaccc gctgaccggc attctgacgc gtgcccaaaa
     3421 cctgtgtgcc gcacaaggat acagtgccga tatctgccgt caggttgcca aagccgccga
     3481 cacgaacgac ctgacactct tcgaaaccga actggatacg tatatagaac gtgtagaaat
     3541 ggccgaatcc gaacttgaca aagcacggca aggcggcgat gcgcaagccg tcgaaacagc
     3601 ccggcacgcc tacctgaacg cactcaaccg tctgtcccga caaatccaca gtttgaaaac
     3661 cggcgttgcc ggcatccgta tgccgaacct ggccgaactg atcagccggt cggccaacac
     3721 cgccgtttcc gaacaggccg cctacaatac cggccggcaa caagcggaac gccgcatcga
     3781 ccgccacctt accgatccgc agcagcaaaa catctggctg gaaaccggta cgcaacaaac
     3841 cgactaccat agcggcacac accgtcccta ccaacaaact accaactatg cacatatcgg
     3901 catccaaacc ggcatcaccg accgtctcag tgtcggtacg attttaaccg atgagcgcac
     3961 aaacaaccgt tttgatgaag gcgtatccgc ccgaaaccgc agcaacggcg cacatctgtt
     4021 cgtcaaaggg gaaaacggcg cactctttgc cgcggcagat ttaggctaca gcaacagccg
     4081 tacccgattt accgattatg acggggctgc cgtccgccgc cacgcatggg atgcaggcat
     4141 caacaccggc atcaaaatcg ataccggcat caacctcaga ccctatgccg gcatccgtat
     4201 aaaccgcagc aacggcaacc ggtacgtact cgacggcgca gagataaaca gcccggcgca
     4261 aatccaaacc acatggcatg ccggcatccg tctcgataaa accgtcgaac tgggtcaagc
     4321 caagctgacc cccgccttca gcagcgatta ctaccatacc cgccaaaaca gcggttccgc
     4381 cctcagcgtc aacgaccgta ccttactgca gcaagccgcc cacggcacac tgcataccct
     4441 gcaaatcgac gccggataca aaggctggaa cgccaaactt catgccgctt acggcaaaga
     4501 cagcaacacc gcccgccaca aacaggcagg aatcaaaata ggctacaact ggtaacaagc
     4561 cgataaaaat gccgtctgga acccgcgttt cagacggcat ttgcgttaaa aatagtaaac
     4621 cgttccaaaa gggagtagaa tagtgccgtt tccaaccctg cgcctgtacc gtcaggcttt
     4681 tattatggac cttcccagtt cgtttttact gaacacccct tccgattcca aacgacaata
     4741 accatcccgc cggaatgcct ccccgcgcac ggcgggcgga gcatttatga gcatcgaacc
     4801 aacccctccg aaccttgaaa acgacggtat cgaaaacgat gtagaacgcg tttccgccga
     4861 tttcgaccgt gtccactccc tctgcgaaat cctcgaacct gcttttgaac aaatcgaaaa
     4921 cggtacaccg ctcgaagacg cgccgctgcg cgacaagctg accgagctga ccgtcctctt
     4981 gagcgagctg caccctgccg acgtggcggc ggtgttggaa tcgctgccgc cgcgcgaacg
     5041 caatatcgtc tggattttag tgaaacccga agacgacggc gaagtattgc tggaagtatc
     5101 cgacgcggtg cgcgaaacgc tgatcgagtc gatggacaag gacgaattgt tggcggcagt
     5161 tgacgatttg gacgcggacg aattggcgga actggcagac gatttgccgc accaagtggt
     5221 ttacgaagcc ttacagacgc gtgatgagga agaacgcgcc caagtcaagg cggcaatgtc
     5281 ctacgaagac aaccaagtcg gtgcgattat ggacttcgag ttggtcagca tccgcgccga
     5341 tgtcgcctgc gaagtggtgc tgcgttacct gcgccgcttc gacagcctgc ccgaccacac
     5401 ggataaaatc ttcgtggttg acgaaaacga tgtgttgcag ggcgtgctgc ccatccgcaa
     5461 acttttggtc gccgatcccg aagacttggt ggaaaacgtg atggcgaaag atgtcgtgcg
     5521 tttccgcgcc gaagacgacg tggaagaagc agcgcaggcg tttgaacgct atgacttggt
     5581 taccgcgccc gtcgtcgatg aaaacaaaaa gctcatcggc agggtgaccg ttgacgaaat
     5641 ggtggacgtg atccgcgaag aatccgaagc agatatgttg aacatggcgg gtttgcaaga
     5701 agaggaagac ttattcgccc ccgtcttgga ttccgtcaaa aaccgctgga tgtggctcgc
     5761 cgtcaacctc tgcaccgcct tccttgccag ccgcgtcatc ggcgcgtttg aaggcagcat
     5821 cgaaaaaatc gtcgcactcg ccgcgctgat gcccatcgtc gccggcatcg gcggcaactc
     5881 gggcaaccag acgattacca tgattgtccg cgccatggcg atggggcaac tgacggatat
     5941 gcaggcgggg cgtttgctga aaaaagaagt cggcgtcgcc ttggtcaacg gcatcatttg
     6001 gggaacggtc atgggcgcag tatcttggct gctttacggc agcctcggca tcggtttggt
     6061 catggttgcc gcgatgacgc tcaacctgct gctggcggca accgtcggcg tattaattcc
     6121 cgtggtaatg gaaaagttcg gacgcgatcc cgcgctgggc agctcggtgc tgattacggc
     6181 ggtaacggat tccggcggct tcctgatttt cttgggtctc gccaccctgt tcctgcttta
     6241 aatgccgtct gaacccgcgc aaaaatgccg tctgaagcgg aagctgcttc agacggcatt
     6301 tgactattta tccttgttgc acaagattat tggacggtat gccggggcag ccctttggca
     6361 acgccgacca catcctcccc gaacagcgcg ttgacatcgg tttcgtcaaa cacatatttg
     6421 ctgtggcaga aatcgcaatc gacttcgatg ctgccttgtt ccaccaccac gccaccgact
     6481 tcttccccgc ccagcatcag cagcatatcg ctgactttgc cgcgcgaaca ggtgcatgaa
     6541 aattcaaacg tttccggctc gaacacgcgc ggcggcgttt cgtggaacag gcggtacaaa
     6601 acatgttgcg catccaattc tgccagctcg tccgccgtca gtgtacgcgc cagcgtactg
     6661 acgtgttccc atgcctcttc atccaacacc gcttcaggca gacgctgcac cagcagaccg
     6721 cccgccgctt cgtcgcttgc agacaggacg atgtgcgtat cgatttgttc ggaacgcttc
     6781 atatagttcg ccaacatttg cgcgataccg ccgccttcca aaggcactac gccctgccag
     6841 ggttcgccgt ctttgggctg cagcgtcagc acgaatacgc cgccctcgcc caaaaggtcg
     6901 ccgaggcttt cgtcatcggc tatttctgcg gtttcgtccc aacgcgcggt tgcacggacg
     6961 gtacggtcgg aagccgcttc cgcaaccagc attttcagcc gcccccgccc ctgaacctgc
     7021 acaatcagcg tgccttcgtt tttgaggttg cccgacagca acacacccgc cgccaacaac
     7081 tcacccaaag cgcggcggat ggcggcggga tagtttttct gttttacaat gtgctgccac
     7141 acgttttcca gacggacgtg cagcccgcgc acgggcatat cgtcgaagat aaagcgggta
     7201 cgcacatcgg cgcggttgat ggcggtttga ttcatgattt tctctgactg attgttcgga
     7261 tggcggctat atggttgcgg tcggcgcgaa aacaagacgg acggcggatg cgcttcccaa
     7321 attatcaata aattatataa aaatcaacat attaactcaa tctaacaagc cgttttttgc
     7381 caaacagccg tttttttata tacaatcaac aagatatttt cgactgatac agcataacat
     7441 cgcacggcgg cacgatgcct cctgcgcgga aacaccgata tggattcttt tttcaaaccg
     7501 gcagtttggg cggttttgtg gctgatgttt gccgtccgcc ccgcccttgc cgacgagttg
     7561 accaacctgc tcagcagccg cgagcagatt ctcagacagt ttgccgaaga cgaacagccc
     7621 gttttaccca tcaaccgagc ccccgcccgg cgggcgggca atgccgacga actcatcggc
     7681 agcgcgatgg ggcttaacga acagcccgtt ttacccgtca accgagtccc cgcccggcgg
     7741 gcgggcaatg ccgacgaact catcggcaac gcgatggggc ttaacgaaca gcccgtttta
     7801 cccgtcaacc gagcccccgc ccggcgggcg ggcaatgccg acgaactcat cggcaacgcg
     7861 atgggacttt tgggtattgc ctaccgctac ggcggcacat cggtttctac cggttttgac
     7921 tgcagcggct tcatgcagca catcttcaaa cgcgccatgg gcatcaacct gccgcgcacg
     7981 tcggcagaac aggcacggat gggtacgccg gttgcccgaa gcgaattgca gcccggggat
     8041 atggtgtttt tccgcacgct cggcggcagc cgcatttccc atgtcggact ttatatcggc
     8101 aacaaccgct tcatccacgc gccgcgcacg gggaaaaata tcgaaatcac cagcctgagc
     8161 cacaaatatt ggagcggcaa atacgcgttc gcccgccggg tcaagaaaaa cgacccgtcc
     8221 cgctttctga actgatttcc caaggaatac gcaatgagta tgcccgaaat gcccaaatgg
     8281 tacgacgatg acggacagat cgtgtcctgt accgaaaagg tcaaagtgat gtccgaaaat
     8341 atggccgagc tgtatcagac ggcacaagac gcgtttgaag acgcgctgct gatgggttgc
     8401 ggcgaacgtc agttgcgcga ttacctgctc gcgctgattg aaggtttgga aaatccctac
     8461 cgcaaagtct gaacacgccc cggttgctgc ggcacggttt atccgtgccg tttttgcgtt
     8521 tgtgcgcggc ttcggctttt cagacggcat atttgacgtt atgattaaac agttaacaag
     8581 atttatcaca acgccgtcaa aagacagaca cacaacatga acatcatccg cgcgctcctc
     8641 atcatcctcg gctgcctcgc cactggcgaa accgccgttt tcctagcagg catcaaactg
     8701 cccggcagca tcgtcggcat gggcgtgctg tttgcgcttt tgcaggcggg ttgggtcaaa
     8761 acgtcttggc tgcaacagct taccgacgcg ctgatggcga atctgacgct gtttctcgtg
     8821 ccgccctgcg tggcggtcat cagctatttg gatttgattg ccgacgattg gttttcgata
     8881 ctggtttccg cctccgccag cactttgtgc gtactgctgg ttacaggcaa ggttcaccgc
     8941 tggatacgga gcattatctg atgaacgaaa tcctcaggca gcccagcgtt ctgcttttcc
     9001 tcacgcttgc cgtgtacgcg cttgcgatta tcgtgcgcac gcgcacgggc aatatcttct
     9061 gcaaccccgt actcgtcagc actatcgtgc tgattgccta cctgaaaatc ctcgacatcg
     9121 attatgcggt gtaccacaac gccgcgcaat tcattgattt ttggctgaaa cccgccgtcg
     9181 tcgtgcttgc cgtgccgctc taccaaaacc gccgtaaaat cttcaaccag tggctgcccg
     9241 tcatcgtttc acagcttgcg ggcagcgtta cgggcattgt tacggggatg tattttgcca
     9301 aatggctggg cgcggaacgc gaagtcgtcc tctcgctcgc gtccaaatct gttaccaacc
     9361 ccatcgccat tgaaatcacc cgctccatcg gcggcattcc cgccattacc gccgccaccg
     9421 tcatcattgc cggtctggtc ggacagattg ccggttacaa aatgctgaag aacacagtcg
     9481 tcatgccctc gtccgtgggt atgtcgctcg gcacggcttc gcacgcgatg gggattgccg
     9541 cctcgctcga acgcagccgc cgtatggcgg catacgcggg actggggctg acgttcaacg
     9601 gcgtactgac cgcgctgatt gcgccgctgc tcatccccgt tttgggattt tgaacccgtt
     9661 tcagacggca tttcagccca tgctgtctga acgccgacac actcgcaagg agaaccgtta
     9721 tggctgtcaa cctgaccgaa aaaaccgccg aacaactgcc cgacatcgac ggcattgccc
     9781 tctacaccgc ccaagcaggc gtgaagaagc ccgggcatac cgacctgaca ctgattgccg
     9841 tagccgccgg cagcaccgtc ggtgcagtct tcacgaccaa ccgtttctgt gccgcgcccg
     9901 tccacatcgc caaatcgcac cttttcaacg aagacggcgt gcgcgccctc atcatcaaca
     9961 cgggcaacgc caacgcgggt acgggcgcac agggcagaat cgatgctttg gcagtgtgtg
    10021 ccgccgccgc ccggcaaatc ggctgcaaac cgaaccaggt gctgcccttc tccaccggcg
    10081 tgattctcga accgctgccc gcagacaaaa tcatcgccgc gctgcccaaa atgcagcctg
    10141 ccttctggaa cgaagcggca cgcgccatca tgaccaccga caccgtgccc aaagccgcct
    10201 cgcgcgaagg caaggtcggc gacaaacaca ccgtccgcgc cacgggcatc gccaaaggct
    10261 cgggcatgat tcatcccaat atggcgacca tgctcggttt catcgccacc gatgccaaag
    10321 tttcccaacc cgtcctccaa ctgatgacgc aggaaatcgc cgacgaaacc ttcaacacca
    10381 tcaccgttga cggcgacacc agcaccaacg acagcttcgt catcatcgcc accggcaaaa
    10441 acagccaaag cgaaatcgac aacatcgccg acccgcgtta cgcccaactc aaagaattgt
    10501 tgtgcagcct cgcgctcgaa ctcgcccaag ccatcgtccg cgacggcgaa ggtgcgacca
    10561 agttcatcac cgtccgcgtc gaaaacgcca aaacccgcga cgaagcccgc caagccgcct
    10621 acgccgtggc acgttcgccg ctggtcaaaa ccgccttttt cgcctccgac cccaacctcg
    10681 gcaggctgct cgccgccatc ggttatgccg gcgttgtcga cctcgatact gacctcgtgg
    10741 aaatgtatct cgacgatatt ttggttgccg aacacggtgg acgcgccgca agctacaccg
    10801 aagcacaagg gcaggcggtg atgtcgaagg ccgaaatcac cgtccgcatc aagctgcatc
    10861 gcggacaaag tgccgccacc gtctatacct gcgacctgtc gcacggatac gtttccatca
    10921 acgccgacta ccgttcctga cccgacacgg cttcagacgg catacataaa atgccgtctg
    10981 aaccgccgga caacatgcca tgacctccac attcccccgc cgcctcgccc gcaaaatccg
    11041 ccaaacccgc cgcctgtcgc gcaaaagcat cgcctttctg ttccttttgg caggttcggc
    11101 actcgtcgcc ctgaccgcgc tgttttttgc ccatcttgcc gattttgcgc tggaactgaa
    11161 cgccaaactg gttcaacaat acccgtggtt cgcgtgggtc gcgcttcctt tgggtttgcc
    11221 gcttattgcg tggctcacac gcaaattcgc ccccttcacc gccggcagcg gcatcccgca
    11281 ggtcatcgcc tcactgtcgc tgccctacgg cgcacagaaa acgcggctga tccgcctcgg
    11341 gcagacgctg ctgaagattc cgctgacctt tttgggtatg ctgttcggcg cgtccatcgg
    11401 acgcgaaggt ccgtccgtgc aggtcggcgc ggcagtgatg ggcgcgtggg gcgcgtggtg
    11461 caagaaacac ggcttggcat tcaaagggat gcaggaaaac gatttgatgg cggcgggcgc
    11521 ggcgggcggt ttggcagccg cgttcaacgc gccgctggcg ggcgtgattt tcgccattga
    11581 ggaactcggg cgcggcatca tgttgcgctg ggagaggcaa attcttttgg gcgtgctcgc
    11641 ctccggtttc atacaggtcg ccattcaggg caacaacccg tatttttccg gcttcaacgg
    11701 cggcgtattg gaacatatct ttctgtgggt cgcactgtcc ggcctggttt gcggcgcggc
    11761 gggcgggctg ttcggacgtt tgctctatcg cggtgcggcg gcgtttgcac cgcgcaagat
    11821 acgcggcttc atccgcaacc gtccgctgct gctggcggca ctgatggggc tgctgctcgc
    11881 cctgctcggc acgttctacc aaggcaaaac ctacggcacc ggctaccacg aagccgccca
    11941 agccctgcac ggcatctacg aagccccctt cggactcgcc gccgccaaat ggctcgccac
    12001 cgtattcagc tattgggcag gcattccggg cggcattttc actccctcgc tgaccatagg
    12061 cgcggttttg ggcgagcata tcgccgccat cgccgacata tcgcagggtg caaacatcat
    12121 cgtcctcatc tgcatggcgg catttctggc gggcgcgaca caatccccga ttacttccgc
    12181 cgtcgtcgtc atggaaatga cgggcggaca aagcctgctg ttttggatgc taattgcctg
    12241 cattttcgcc tcgcaggttt cgcgccagtt ttcgccgcgt ccgttctacc acgcatcggg
    12301 aatgcgcttc cgccagcgcg tgcttcaaga aaccgccgcc caaaccggca atgcgcccgc
    12361 aagaccgcaa acagcaaaca gcaaaacggg aatgccgtct gaaaattaaa acgcccccga
    12421 tcaaacgccg gcagccgcct tgatttgaat accgttccgc cgccgcttga aatttcagca
    12481 acaatgccgt ctgaacgaca gaatgcggtt ttcagacggc atttccacat cccgatattg
    12541 cctaaacaaa accgaagcgt ttgctataat tctatttttt accgcacacg caccaatcat
    12601 gtttcccgat ttctcccaaa ccctctccaa agaccgccac ttcctacgtt ccgccttcaa
    12661 aaatcccaac aaatacggcg gcttgtccaa agtcgaagaa aaataccaaa aatcgcacga
    12721 aatctttttg aagcgtttgg ctgccttgcc caaacccgaa ttcgataaca ccctgcccgt
    12781 tcacgagaag ctcgacgaaa tcaaaaaagc cattgccgag aatcaggtaa cgattatttg
    12841 cggcgaaacc ggttcgggca agaccactca gttgcccaag atttgtctgg aactcgggcg
    12901 cggggcggcg ggtttgatcg ggcataccca gccgcgccgt ttggcggcgc gttccgtagc
    12961 agagcggatt gccgaagagc tgaaatccga aatcggcagc gcggtcggct ataaagtgcg
    13021 cttcaccgac cacacctcgc gcgatgcctg cgtcaagctg atgaccgacg gcatcctgct
    13081 ggcggaaacc cagaccgacc gttatctcgc cgcctacgac acgattatca tcgacgaagc
    13141 gcacgagcgc agcctgaaca tcgacttcct tttgggctat ttgaaacaac tcctgccgcg
    13201 ccgccccgat ttgaaagtca tcatcacctc ggcaacgata gacgcagaac gcttttccca
    13261 acacttcaac ggcgcgcccg tgctggaagt gagcggacgc acctatcccg tcgaaatcct
    13321 ctaccgaccg ctgatcggca aagacgaaga cgacgcggaa atagaattga ccgacgcgat
    13381 tgtcgatgcg gcggacgaat tggcgcgata tggcgaaggc gatattttgg tattcctgcc
    13441 gggcgaacgc gaaatccgcg aagccgccga agccctgcgc aaatccaccc tgcgccgtaa
    13501 cgacgaaatc ctgcccctgt tcgcacgcct gtcgcacgcc gaacagcaca aaatcttcca
    13561 cccctcaggc gcgaaacgcc gcatcgtatt ggcaaccaac gtcgccgaaa cctcgcttac
    13621 cgtgccgggc atcaaatacg tcatcgacac cggtctcgcg cgcgtcaaac gctattccgc
    13681 acgggcgaaa gtggagcagc ttcatgtcga aaaaatctcc caagccgccg cccgccaacg
    13741 ctccggccgc tgcggacgcg tatccgcagg cgtatgtatc cgactgtttt cagaagaaga
    13801 tttcaacagt cgccccgaat ttaccgaccc cgaaatcgtc cgcagcaacc tcgccgccgt
    13861 catcctgcgc atggcatcgc tgaaactcgg cgacgtagcg gcattcccat ttttagaaat
    13921 gcccgattcg aggtatatta atgatgggtt tcaggtgttg ctggaattag gggcagtgga
    13981 ggccgcctga aaacaagtag gatatacact tctacctgac aaagaccaac aaacgataaa
    14041 actacggcac atctagatgc ccaacctaca acattgcaca cggtaaaaac atgaaacact
    14101 tactcatcga ttttgaaaac gtccagccgc aaaacttaga caaattacta accgaaaata
    14161 cccatatttg gctatttata ggtgtattac acaaaatgtt acctattagt ctggtgcaat
    14221 ccctactacg tttcggcgaa cgtgtccatc ttgtccagtt acaaaaaacg gggaaaaacg
    14281 cattggattt ttacctgtcc tattacctcg gacaaattac cgccacagac cccaatgccc
    14341 aaatcggcat actctcgcgt gatggaggat acgatgttct ggtcgaacat attttgaaaa
    14401 accaccaggc gaagggtatc gtgcgcctag ccaatataga tgaagtacaa catcagaaaa
    14461 ttgctaccga accgccgtca gcattgcggg aaaacactcc tcagcctgaa accaccctca
    14521 aaccacagca accattaact tcctatttcc aagcagccct aactgcactg cgccgccccg
    14581 acgctttccg cccctgccgc ctgcataacc tgcgacaaaa tctgcgtaag catattttga
    14641 gtgatttgtt taaagaaaaa accgatgaag aatgcgaaat aaccactgct aacgttatca
    14701 ataaactcaa agcacaaaac ttcatcagca ttgatgaaca ggaaaccgtt tcctaccatc
    14761 tcagtgataa tgatttgtta caaagaatcc aacgccatat tttaagccaa cgtcccaaaa
    14821 cctacgctga ttttcaagcc gtcgtgcaaa accgagcaga tgcacttcac ttaacagtcg
    14881 gtaccaacga cattcaatcc tttgcgcgac atttgcgcga ccaaaacctg atccgccaaa
    14941 acaatgggaa aattgaatat gcaccgttta ctgaacctaa accacagcca acgcccaagc
    15001 agcctaaaaa aaccgcatgg gaacctgatg aaattatttg gaaaaaagtg attgccgcgt
    15061 tatcgttaaa gaaccgtcct aataaaacca aaactttacg caatacaatc caggcactca
    15121 caaaatccaa tgcacaagaa actgacaaac tgctacaaca tttacaagat acccaagtcc
    15181 tacgtattga cggtactaaa attgtgtata tcaagtagga tgggcattta tgcctgacct
    15241 acaaaaccaa aaacaaccta acacccttaa tcaactccac aaaccctttt cagacaacct
    15301 cgttttttga aaaacaatct gtaaacagat aactgctgaa gaataccgtt gccgagcccc
    15361 aaaacctgcc tgcaactttt attgtgaact tcccattatg aaaaaatccc ttttcgttct
    15421 ctttctctat tcatctctac ttaccgccag cgaaatcgcc tatcgctttg tattcggaat
    15481 tgaaacctta ccggctgcaa aaatggcaga aacgtttgcg ctgacattta tgattgctgc
    15541 gctgtatctg tttgcgcgtt ataaggcttc gcggctgctg attgcggtgt ttttcgcgtt
    15601 cagcattatt gccaacaatg tacattatgc ggtttatcaa agttggatga cgggcatcaa
    15661 ttattggctg atgctgaaag agattaccga agttggcggc gcgggggcgt cgatgttgga
    15721 taagttgtgg ctgcctgcgt tgtggggcat gttggaagtc atgttgtttt gcagccttgc
    15781 caagttccgc cgtaagacgc atttttctgc cgatatactg tttgccttcc taatgctgat
    15841 gattttcgtg cgttcgttcg acacgaaaca agagcacggt atttcgccca aaccgacata
    15901 cagccgtatc aaagccaatt atttcagctt cggttatttt gtcggacgcg tattgccgta
    15961 tcagttattt gatttaagca aaatccctgt gttcaaacag tctgcgccaa gcaaaatcgg
    16021 gcagggcagt gttcaaaata tcgtcctgat tatgggcgag agcgaaagcg cggcgcattt
    16081 gaaattgttt ggctacgggc gcgaaacttc gccgttttta acccagctgt cgcaagccga
    16141 ttttaagccg attgtgaaac aaagttattc cgcaggcttt atgacggcag tatccctgcc
    16201 cagtttcttt aacgtcatac cgcatgccaa cggcttggaa caaatcagcg gcggcgatac
    16261 caatatgttc cgcctcgcca aagagcaggg ctatgagacg tatttttaca gtgctcaggc
    16321 tgaaaaccaa atggcaattt tgaacttaat cggtaagaaa tggatagacc atctgattca
    16381 accgacgcag cttggctacg gcaacggcga caatatgccc gatgagaagc tgctgccgct
    16441 gttcgacaaa atcaatttgc agcagggcag gcattttatc gtgttgcacc aacgcggttc
    16501 gcacgcccca tacggcgcat tgttgcagcc tcaagataaa gtattcggcg aagccgatat
    16561 tgtggataag tacgacaaca ccatccacaa aaccgaccaa atgattcaaa ccgtattcga
    16621 gcagctgcaa aagcagcctg acggcaactg gctgtttgcc tatacctccg atcatggcca
    16681 gtatgttcgc caagatatct acaatcaagg cacggtgcag cccgacagct atctcgtgcc
    16741 gctggtgttg tacagcccgg ataaggccgt gcaacaggct gccaaccagg cttttgcacc
    16801 ttgcgagatt gccttccatc agcagctttc aacgttcctg attcacacgt tgggctacga
    16861 tatgccggtt tcaggttgtc gcgaaggctc ggtaacgggc aacctgatta cgggcgatgc
    16921 aggcagcttg agcattcgcg atggcaaggc ggaatatgtt tatccgcaat aagcgacgtg
    16981 aaaaccaata aaaacaaatt tagatgatgt cggggaagat gcccgaccga caagactatg
    17041 caaaatatga aaaaccaagt acgcggatca ggcatggatg cacgatccaa tccggccaat
    17101 gtttcagacg gcctgcaaaa cagttcgggt catatcggta ccaacacgcg ttaccgcctg
    17161 accaaactcg gcgaacaaat ggcgcgcctg cccatcgacc cgaaaatcgc gcgcattttg
    17221 ttggcggcga agaaacacga ctgcatggcg gaaatattgg tgatcgcgtc cgcactgtcg
    17281 attcaagacc cgcgcgagcg gccgctggaa gcgcgcgatg ccgccgccaa ggcgcacgag
    17341 cgttttaccg acaagcagtc cgatttcctt gcctatctga acatttggga cagcttccag
    17401 cgcgagcgcg acaagggctt gtccaacaag cagttggtgc agtggtgccg tcaatatttc
    17461 ctgtcgcacc tgcggatgcg cgagtggcgc gagctgcacc accagcttgc ccaaaccgcg
    17521 attgaaatgg gtttgaccac caaggaagcc gctttcagac gacctcccga aatcaggcag
    17581 ctcacgtcgt ctgaaaacca aggcgaccaa gacctatccg ccaaactcaa gcaaaaacaa
    17641 ctggataaga aacaacaccg cgcccaaatc cgcgctgcca aagaagcggg ctacgaacaa
    17701 atccaccgcg ccctgctcac cggcctcatc gccaacgtcg gcatgaaatc gcccgacggc
    17761 aacgactaca ccggcgcgcg cggcagccgc ttccaccttt tccccgcctc cgccctgttc
    17821 aaagccaaac ccaaatgggt gatggcggca gaactcgttg aaacgacaag actttatgct
    17881 cgcgacgtgg ccgccatcca gcccgaatgg atcgagcagg aagcgccgca cctcgtccgc
    17941 taccactatt tcgagccgca ctgggagcaa aaacgcggcg aagttgtcgc cggcgaacgc
    18001 gtgacgcttt acggcctgac cgtattgccg cgccgccccg tgtcttacgg caaagttgcg
    18061 cccgaagaag cgcgcgaaat ctttatccgc agtgcgctgg tggcgcagga atgcgatttg
    18121 aaagcggact tttttgtcca caacaaaaag ctgattaaag aaattaccga actcgaacac
    18181 aaatcgcgca agcaagacgt attggtcgac gacgaagccc tgtttgcgtt ttatcacgaa
    18241 cggctgcccg atttttatac ggcggatgcg gtttcagacg gtctgcgtcc tgcaaatccc
    18301 cagcaaactg ccccctccta cgcgagggaa gaacggagag agggcaaaac agttgccgca
    18361 caaaccaact tctccgcaac cgcagcaaac cctctcccta accctctccc acaggagagg
    18421 gagcagagtg ccgcagcttc agcggtttca aacgacctgc atcctgcaaa tcccca
//
LOCUS       DAXHSF010000022        17656 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.022, whole genome shotgun sequence.
ACCESSION   DAXHSF010000022 DAXHSF010000000
VERSION     DAXHSF010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17656
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.022"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(108..809)
                     /locus_tag="ACFM8S_000619"
     CDS             complement(108..809)
                     /locus_tag="ACFM8S_000619"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFC6313726.1"
                     /translation="MSLKTVAVIGAMEQEIELLREMMENVKAVSFGKFAAYEGELAGK
                     RMVLALSGIGKVNAAVATAWIIREFAPDCVINTGSAGGLGKGLKVGDVVVGTEIAHHD
                     VDVTAFGYVWGQVPQLPAVFVSDGLLVGKAKQAAEVFECAAVEQGLIVSGDRFVHSSE
                     GVAEIRKHFPKVKAVEMEAAAIAQTCHQLETPFVIIRAVSDSADEKADISFDEFLKTA
                     AASSAKMVAEIVKSL"
     gene            977..2107
                     /locus_tag="ACFM8S_000620"
     CDS             977..2107
                     /locus_tag="ACFM8S_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HFC6313727.1"
                     /translation="MFTDENMTAKEELFAWLRHMNQNKGSDLFVTTHFPPAMKLDGKI
                     TRITDEPLTAEKCMEIAFSIMSAKQAEEFSSTNECNFAISLPDTSRFRVNAMIQRGAT
                     ALVFRAITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNEN
                     SFGHIITIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDR
                     ETMDYAIAFAETGHLCMATLHANSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQ
                     RLVPRDGGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKSTTLGMQTFDQHLYQL
                     YEKGDISLQEALKNADSAHDLRLAVQLRSRRAQSSSPDLELL"
     gene            2141..3118
                     /locus_tag="ACFM8S_000621"
     CDS             2141..3118
                     /locus_tag="ACFM8S_000621"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFC6313728.1"
                     /translation="MIYPWHNEQWRQIAEHWERRPNAWLFAGKKDTGKTTFARFAAKA
                     LLCETPAPGCKPCGECMSCHLFGQGSHPDFYEITPLSDEPENGRKLLQIKIDAVREII
                     DNVYLTSVRGGLRVILIHPAESMNVQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTI
                     KSRCRKMVLPAPSHEEALAYLRERGVAEPEERLAFHSGAPLFDEADGVRALRIKLLDI
                     LAEPRLLKILDYAALFDKEKLPLAVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLLQ
                     TVSGFRPRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLELKKENG"
     gene            3122..3472
                     /locus_tag="ACFM8S_000622"
     CDS             3122..3472
                     /locus_tag="ACFM8S_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HFC6313729.1"
                     /translation="MSDGQNIPAKMMSLQLKDMNLLYSSYMPFLEHGGLFVQTNDVFS
                     IGDDILLAVEILNFPKLFLPTKVAWINPARTSSKPKGVGLAFTKHENCLKVKDQIEVE
                     LGNTIGGSRPTFTM"
     gene            3477..4256
                     /locus_tag="ACFM8S_000623"
     CDS             3477..4256
                     /locus_tag="ACFM8S_000623"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFC6313730.1"
                     /translation="MHIIDSHCHLNFEGLKERLPEVLSNMEANGVGQALAISVSRESF
                     SEVFAIAEAHEHIYCTIGVHPDSKEAEEFSIAEMVAAAAHPKVVGIGETGLDYYWCKG
                     DLSWQHKRFADHIEAANQTGLPVIVHTRDAAADTLSILKECRVNSGVIHCFSEDIGFA
                     RAAMDLGLYISFSGIVTFKNAPLVQEAAKYVPDDRILVETDAPFLAPVPKRGRQNEPA
                     FVRYTAEHIAKLRNQTLEQVAAYTTENFYRLFQKVPDMRTV"
     gene            4282..4497
                     /locus_tag="ACFM8S_000624"
     CDS             4282..4497
                     /locus_tag="ACFM8S_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFC6313731.1"
                     /translation="MKAIHPYACPRCCRLPANTFRTGMANSASKFCIAKGGRREVKKD
                     ESGGGYALCHLPDSRIVEEWEYFRSQH"
     gene            4598..4909
                     /gene="grxD"
                     /locus_tag="ACFM8S_000625"
     CDS             4598..4909
                     /gene="grxD"
                     /locus_tag="ACFM8S_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HFC6313732.1"
                     /translation="MASIHDQIKEVVTTHRVVLFMKGTKQFPQCGFSSRAVQILNAAG
                     CTDYVTVNVLENPEVRQGIKEYSDWPTIPQLYVNGEFVGGSDILMEMYEAGELQELLK
                     A"
     gene            5015..5641
                     /gene="upp"
                     /locus_tag="ACFM8S_000626"
     CDS             5015..5641
                     /gene="upp"
                     /locus_tag="ACFM8S_000626"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006223 - uracil salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFC6313733.1"
                     /translation="MNVNVINHPLVRHKLTLMREADCSTYKFRTLAIELARLMAYEAS
                     RDFEIEKYLIDGWCGQIEGDRIKGKTLTVVPILRAGLGMLDGVLDLIPTAKISVVGLQ
                     RDEETLKPVSYFEKFVDSMDERPALIIDPMLATGGSMVATIDLLKAKGCKNIKALVLV
                     AAPEGVKAVNDAHPDVTIYTAALDSHLNENGYIIPGLGDAGDKIFGTR"
     gene            5664..5984
                     /locus_tag="ACFM8S_000627"
     CDS             5664..5984
                     /locus_tag="ACFM8S_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HFC6313734.1"
                     /translation="MNFQDYLATFSSIDHLGGLDVQDADGKTVHHIPAVQGKLGSLKL
                     YNALAERFDGKLDKEAAEQGLIWFAEHVADARAHPGKHPNIDLLEKVVQSGETLLLKP
                     LAAQ"
     gene            6235..6654
                     /locus_tag="ACFM8S_000628"
     CDS             6235..6654
                     /locus_tag="ACFM8S_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Gly1ORF1"
                     /protein_id="HFC6313735.1"
                     /translation="MKKMFLSAVLLLSAATRTVWADTVFSCKTDNNKYIEVQKINRNL
                     YEYSFGSAAKKEIAIRNSKADLLGRSDRWQGMGSGRWATMKFQNGEFMYTIWTGFDSV
                     THTESSGVVVERRGKEVARVGCTPKTAQANFNDDDFS"
     gene            6675..7436
                     /locus_tag="ACFM8S_000629"
     CDS             6675..7436
                     /locus_tag="ACFM8S_000629"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFC6313736.1"
                     /translation="METAKPVMLIVRPSGRAAEDVETCLNAGWRAEVLSPVEIEADAA
                     GLELLSEQYARADAVFWVSPTAVETAVPYLNLSDGIKMHIAVGKGSRRALERCLGRTV
                     IAPDDGNDSEAVLRLPVWNSLPEGARVLFVRGHGGRDFLMNALQEKGLRTEMAEVYFR
                     RHKPLNFQNFQTENIAAAYITSTELVRLLFGQLPPQFSRFFKSLLYFTHHPRIAEALK
                     REGVCSVETVPTLEAALSHSSISVSDGMVFPGTSN"
     gene            7511..8785
                     /locus_tag="ACFM8S_000630"
     CDS             7511..8785
                     /locus_tag="ACFM8S_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFC6313737.1"
                     /translation="MPSETSSPRKENETEVHIPAAPFIVKQSGSNALAVCALVLAALG
                     LGTSGFLFVQGQNVLKNQELAFNQKIDKAALGESENAALLKDNLNRQAAIQSELDRLD
                     GNVKANGEQILEMQKSYRELTKGRADWLVDETETILNLAAQQLVLTGNIQTAVGVLEH
                     IDSRLSRFNQAELLPIKQAVSSDLAELKNRPYVDISGTALRLDRLEIAVSGLPLILDG
                     VLKPSVQARNEAVSASWWQNVWEKSLGTLKGLVEIRRLENNDAMLISPEQAYFVRENL
                     RLRLLDARTALMQRNSEVYQGDLNNAETAVRQYFDAKSPATQSWLKELAELKALDVRM
                     TADDGLKNSLNAVRAYRDGTRMTTAENQEAEQATSEPANENTASEPAAASDVKTIEAP
                     SLPSERKPEQPAKKQPVPEKAGRSPSAKGERA"
     gene            8782..9999
                     /locus_tag="ACFM8S_000631"
     CDS             8782..9999
                     /locus_tag="ACFM8S_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HFC6313738.1"
                     /translation="MKTVVWIVVLFAAAVGLALASGIYTGDVYIVLGQTMLRINLHAF
                     VLGSLIAVVVWYFLFKFIIGVFNIPEKMQRFGSARKGRKAALALNKAGLAYFEGRFEK
                     AELEASRVLVNKEAGDNRTLALMLGAHAAGQMENIELRDRYLAEIAKLPEKQQLSRYL
                     LLAESALNRRDYEAAEANLHAAAKMNANLTRLVRLQLRYAFDRGDALQVLAKTEKLSK
                     AGALGKSEMERYQNWAYRRQLADAADAAALKTCLKRIPDSLKNGELSVSVAEKYERLG
                     LYADAVKWVKQHYPHNRRPELLEAFVESVRFLGEREQQKAIDFADAWLKEQPDNALLL
                     MYLGRLAYGRKLWGKAKGYLEASIALKPSISARLVLAKVFDETGEPQKAEAQRNLVLA
                     SVAEENRPSAETH"
     gene            10074..11138
                     /gene="hemE"
                     /locus_tag="ACFM8S_000632"
     CDS             10074..11138
                     /gene="hemE"
                     /locus_tag="ACFM8S_000632"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFC6313739.1"
                     /translation="MTLLKNDTFLRALLKQPVEYTPIWMMRQAGRYLPEYKATRAKAG
                     SFLDLCKNTELATEVTIQPLERFDLDAAILFSDILTVPDAMGLGLYFAEGEGPKFKRA
                     MQHEADIAKLHVPDMEKLQYVFDAVTSIRKALDGRVPLIGFSGSPFTLACYMVEGGGS
                     KEFRTIKTMMYSRPDLLHKILDTNAQAVTAYLNAQIDAGAQAVQIFDTWGGVLSDAAF
                     KEFSLKYIRQIVAGLKRESEGRRVPVIVFAKGGGLWLESMAQIGADALGLDWTCNIGE
                     ARRRVGKQVALQGNFDPFALFGTPESIRTEVARILADYGHGSGHVFNLGHGINQHADP
                     EHAKILVDTVHELSRQYHGG"
     gene            11324..12703
                     /gene="radA"
                     /locus_tag="ACFM8S_000633"
     CDS             11324..12703
                     /gene="radA"
                     /locus_tag="ACFM8S_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFC6313740.1"
                     /translation="MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESLAAPEPK
                     NARFQSWAADTSTVQSLSAVTAAEVPRNPTGMSELDRVLGGGLVDGAVILLGGDPGIG
                     KSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRAQRLELPTEGVNLLAEIRMEAIQTA
                     LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTK
                     DGAIAGPRVLEHMVDTVLYFEGDQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVS
                     NPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFTPKRLTVGLEQNRLA
                     MLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPMPEKTVVF
                     GEIGLSGEVRPVARGQERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIYGVSSLQEA
                     IDICRDSRE"
     gene            complement(12979..13458)
                     /locus_tag="ACFM8S_000634"
     CDS             complement(12979..13458)
                     /locus_tag="ACFM8S_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HFC6313741.1"
                     /translation="MKHILPLIAASALCISTASAHPASEPSTQNETAMTTHTLTSKYS
                     FDETVSRLETAIKSKGMDIFAVIDHQEAARRNGLTMQPAKVIVFGTPKAGTPLMVKDP
                     AFALQLPLRVLVTETDGKVRAAYTDTRALIAGSRIGFDEVANTLANAEKLIQKTIGE"
     gene            complement(13590..13949)
                     /locus_tag="ACFM8S_000635"
     CDS             complement(13590..13949)
                     /locus_tag="ACFM8S_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFC6313742.1"
                     /translation="MNIKHLITAALIASAAFAAQAAPQKPVSAAQTAQHPAVWIDVRS
                     EQEFSEGHLHNAVNIPVDQIVRRIHEAAPDKDTPVNLYCRSGRRAEAALQELKKAGYT
                     NVANHGGYEDLLKKGMK"
     gene            complement(13988..17602)
                     /gene="recB"
                     /locus_tag="ACFM8S_000636"
     CDS             complement(13988..17602)
                     /gene="recB"
                     /locus_tag="ACFM8S_000636"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFC6313743.1"
                     /translation="MSAPIQAFDPLTVPIAGTNLIEASAGTGKTYGIAALFTRLIVLE
                     QKNVERVLVVTFTKAATAELKTRLRARLDDVLQVLESKEIAKLGDDTLSDGIAAYCAE
                     HHKGDTFLPALLKQALQKEGRTRLIVRLKAAIGQFDNAAIYTIHGFCQRILRDYAFLC
                     QAPFDVELTEEDGDRLLVPAQDFWRERVSNDPVLAALAFKRKAVPQTVLAQIRAYLSR
                     PYLNFRRPQTDLPQARDAALASWQNICGNLAELKETFWRIHPLLNGNSYRKNSFAELF
                     DALEQKALSPDLPFLEAKLHERLLKLASDKLEAGLKKGKTPDAAVFAELQKLADFRRD
                     LDALEEAEEATIIRLQLDLIGYLNRSLAEMKKSRRERGFDDLLLDVHTALTDNPHAET
                     LARAVAENWETALIDEFQDTDPLQYEIFQKIFIAQNRPLFLVGDPKQAIYSFRGADIY
                     AYLQAAEDARHRYTLATNYRSHAALIGSIGALFRLKERPFVLENIGYSEVGAARAESR
                     LSPKRPAVQVRWLHENDNEKANKDVLRRRAADYCADEIAHALNEAAGGRLNFKGCPLQ
                     SGDIAVLVRTHNEAVMVSAALKKRQVQSVLLSRESVFASPEAAALSALIGFWLEPRRA
                     GTLRFVLTSSIFGYNAQQLHDFNQNESEILHWAESARTALDNWNQYGIFAAMQQFSQT
                     HGIETRLLSRNNGRSLTNYFQLLELLAAEDAQNRNPAALHKWLRDQISLAGNNGGDNR
                     AIRLESDEDLVKIVTMHASKGLQYPLVYCPFAWDAQDTGPSDWQILHQSANRTELLAK
                     AQLSEDEQKQYADEEMAERLRLLYVALTRAEEQLNIYAAYSTNTADNPLAYLIEGSPQ
                     DSRETVRRAYACEKDGIAMLKRNWRRVADNAPSGTNFAFTENAPPPAAYRSNAGQTAE
                     FAANSIPERGFRFVRHTSFTALSRHTQTPDGGEEDACPSLDAAETSVPAMPSETPTAS
                     DGISIHDFPKGTQAGLCLHEILEDFKFGQAAAGQETLIADKLKKYGFEEIWLPAVAEM
                     AEACRKTPLTGAYCLSDIPPECRCPEMGFTLHTEDFGLKRLRDWFARDDIRLPEVCRA
                     AAETLDFHTVNGFLNGFIDMVCQDPDGNICIIDYKSNHLGADASAYTQQAMDEAVTHH
                     HYYLQALIYAVAAARYFKLRGQPPAAVSVRYLFLRGTDGKGGGVWRWDIDAAALEQIK
                     "
BASE COUNT         4041 a         4635 c         5015 g         3965 t
ORIGIN      
        1 aaatgccgtc tgaacaaaca gcggtgcgtc cgaatatcgg gcgcaacgtg gaaatagccc
       61 gatattctaa cggaaaaccg ctgttttggc atgatttgat aaaggtttta taaagatttg
      121 acgatttctg ccaccatttt tgcagaactt gccgccgccg ttttcaaaaa ttcgtcaaag
      181 ctgatgtctg ccttttcgtc cgccgaatcg gaaaccgcgc ggatgatgac gaaaggcgtt
      241 tccaactgat gacaggtttg ggcaatcgcc gccgcttcca tttccactgc tttgactttg
      301 gggaagtgct tgcggatttc cgccacgcct tcgctgctgt ggacgaagcg gtcgccgctg
      361 acaatcaggc cttgttctac cgccgcgcat tcaaacactt ccgccgcctg ttttgctttg
      421 ccgaccaaga ggccgtctga aacaaatacg gcgggcagtt gcggcacttg tccccaaaca
      481 tagccgaatg cggttacatc gacatcgtgg tgcgcgattt ctgtgccgac cacgacatcg
      541 ccgactttca aacccttgcc caagccgccc gcgctgccgg tgttgataac gcagtccggt
      601 gcgaattcac ggataatcca agccgttgca accgccgcgt tgaccttgcc gatgccgctc
      661 aatgcaagca ccatgcgttt tcccgctaat tcgccttcat aggcggcaaa cttgccgaaa
      721 gagacggctt tgacattttc catcatctcg cgcaaaagct cgatttcttg ttccattgcg
      781 ccgataacgg ctactgtttt caaagacata ttgctgacct gttgtgaatt tcggatagaa
      841 tgcctgatta tacacgctaa cacggcagga ttgagtggag gtggtttgtc cgtgccgtct
      901 gaaacggttt cagacggcac ggcgggtttt tggtagaatg ggaaggtaca gattgtttga
      961 agattagggg acgaggatgt ttaccgatga aaatatgacc gcaaaggaag aactgttcgc
     1021 atggctgcgc catatgaacc aaaacaaagg ttccgacctg ttcgtgacga ctcatttccc
     1081 gcccgcaatg aagctggacg gcaaaatcac ccgcatcacg gacgaaccgc tgacggcgga
     1141 aaaatgtatg gaaatcgcct tttcgattat gagtgcgaag caggcggaag aattttcatc
     1201 gaccaacgag tgcaacttcg ccatcagcct gccggacacc agccgcttcc gcgtcaatgc
     1261 gatgatacag cgcggcgcga cggcgttggt attccgtgcg attaccagca agattcccaa
     1321 gtttgaaagc ctgaacctgc cgccggtctt gaaggatgtc gcgctgaaaa aacgcgggct
     1381 ggttattttt gtcggcggca ccggctcggg caaatcgact tcgcttgcct cgcttatcga
     1441 ttaccgcaat gaaaattcgt tcggacacat catcaccatc gaagacccga tcgagtttgt
     1501 ccacgaacac aaaaactgca tcatcaccca gcgcgaggtc ggcgtggata cggaaaactg
     1561 gatggcggcg ttgaaaaaca cgctgcgtca ggcgccggat gtcatcctta tcggcgaaat
     1621 ccgcgaccgc gaaacaatgg actacgccat tgcctttgcc gaaacggggc atttgtgtat
     1681 ggcgacgctg cacgccaaca gcaccaatca ggcactcgac cgcatcatca actttttccc
     1741 cgaggagcgg cgcgaacaat tgctgacgga tttgtcgctc aaccttcagg cgtttatttc
     1801 gcaacgcctc gttccgcgag acggcggcaa gggcagggtg gcggcagtcg aggtgctgct
     1861 caattcgccc ctgatttcgg agttgattca caacggcaac atccatgaaa tcaaagaagt
     1921 gatgaaaaaa tccactaccc tgggtatgca gaccttcgat caacaccttt accaattgta
     1981 tgaaaaaggc gatatttccc tgcaagaagc attgaaaaat gccgattccg cacacgattt
     2041 gcgtttggcg gtacagttgc gcagccgccg cgcgcaaagt tccagccccg atttggaact
     2101 gctctgatgg cggtatggat ttccggacgg atggtttgaa atgatttatc cgtggcataa
     2161 tgagcaatgg cggcagattg cggaacattg ggagcgtcgt cccaatgcat ggctgtttgc
     2221 cggcaaaaaa gatacgggga aaactacatt tgcccgcttt gcggcgaagg cactgttgtg
     2281 cgaaacccct gcaccgggct gcaaaccctg tggcgaatgt atgtcctgcc atctgtttgg
     2341 acagggaagc catcccgatt tttacgaaat cacccccttg tcggacgaac ccgaaaacgg
     2401 acgcaaactg ttgcagatca aaatcgatgc cgtcagggaa atcatcgata atgtgtacct
     2461 gacttcggta cggggcggtt tgcgcgtgat tctgattcat cctgcggaaa gtatgaatgt
     2521 ccaagccgcc aacagtttgt tgaaagtgtt ggaagagccg ccgccacaag tggtcttttt
     2581 gctggtcagc cacgcggcgg acaaggtttt accgaccatt aaaagccgct gccggaagat
     2641 ggttttgccc gcgccttccc atgaagaggc attggcatat ctgcgtgaaa ggggtgtggc
     2701 ggaacctgag gaacgtctgg ctttccattc cggagcgccg ctgtttgatg aggcggacgg
     2761 tgtccgtgcg ttgcggatta aactgttgga tattttggca gaaccaaggt tgttgaagat
     2821 tttggattac gccgcgcttt tcgataagga aaaacttccg ctcgccgtat ttgtcgggtg
     2881 gatgcagaag tggctggtcg atttgggatt gtgcctgcaa cacatgaaac ccgtctatta
     2941 tcccgcttat gaagacaggc tgcttcagac ggtatccggt ttccgtccgc gcaatgtatt
     3001 tgcggcggag gatatgctca aacagcttgc cccctacggg tttcatactt taaatgtcaa
     3061 aatgcagatc gagcatctgc tcatcaacta tttggaattg aagaaagaga acgggtgaat
     3121 tatgtcagac ggacaaaata ttccggcaaa aatgatgtcg ttgcagctga aagacatgaa
     3181 tctgctgtac agctcctaca tgccgttttt ggaacacggc ggtctgtttg tgcagaccaa
     3241 cgacgtattt tccatcgggg acgatattct gcttgccgta gaaatcctca acttccccaa
     3301 actgttcctg ccgaccaaag tcgcctggat caatcctgcg cgtacttcct ccaaacccaa
     3361 aggggtaggg ctggcattca caaaacacga aaactgcctg aaagtcaaag atcagatcga
     3421 agtcgaactg ggcaacacaa tcggcggcag cagacctacg tttaccatgt aacgccatgc
     3481 atatcatcga ttcgcactgc cacctcaatt ttgaaggttt gaaagaacgc ctgcccgaag
     3541 ttttgtccaa catggaagca aacggcgtgg ggcaggcgct cgccatcagc gtcagcaggg
     3601 aaagcttctc cgaagtcttt gccatcgccg aagcgcacga acacatctat tgcaccatag
     3661 gcgtacatcc cgacagcaag gaagccgaag aattttctat tgcggaaatg gttgccgcag
     3721 ccgcccatcc gaaagtggtc ggcattggcg agacgggctt ggattattac tggtgcaaag
     3781 gcgatttgtc ctggcagcac aaacgctttg cagaccacat cgaagcagcc aatcaaaccg
     3841 gactgcccgt tatcgtccat acgcgtgatg cggcggcgga caccttgtct atcctgaaag
     3901 aatgccgggt taattcgggc gttatccact gtttttccga agacatcggt tttgcccgtg
     3961 cagcaatgga tttggggctt tatatttctt tctcgggaat cgttaccttt aaaaacgcac
     4021 ccttggttca ggaggcggcg aaatacgttc cggacgaccg cattttagtg gaaaccgatg
     4081 cgccgttcct cgcccccgta cccaagcgcg gcaggcagaa cgaaccggct ttcgtgcgtt
     4141 atactgccga acatatcgcc aaattgcgga accaaacctt ggaacaggtt gcggcataca
     4201 cgacggaaaa cttttaccgg ctgtttcaaa aagtacccga tatgcggacc gtctgaccct
     4261 gtaccgacga taaggaaaac catgaaggca attcatccgt atgcatgtcc gcgctgctgc
     4321 cggctgcctg ccaacacgtt tcggacaggc atggcaaatt ccgcttccaa attctgcatt
     4381 gccaaaggcg gcagacggga agtaaaaaaa gacgaaagcg gcggcggata tgccctgtgc
     4441 catttgccgg acagcaggat tgtcgaggag tgggaatatt tccgttcaca acattgatac
     4501 tgcgcgatat acggcaaata ttgtgggaag tttccgcttt tgcgtataat gcgtcctacc
     4561 tgacaaattt tgtcaacttt atcaaaagga ataagcgatg gcttccatcc acgaccaaat
     4621 taaagaagta gtaacgacac accgcgtcgt attgtttatg aaaggtacga agcagtttcc
     4681 gcaatgcggt ttctcttccc gcgccgtgca aatcctgaac gcggcaggct gcaccgatta
     4741 cgttaccgtc aacgtattgg aaaatcccga agtacgccaa ggcattaagg aatacagcga
     4801 ctggccgacc atcccccaac tttatgtgaa cggcgagttt gtcggcggtt cggacatcct
     4861 gatggaaatg tatgaggcag gcgagctgca agagctgctg aaagcctgat ggattcggca
     4921 atgccgtctg aacgtgtttc agacggcatt ttcttttccg gcaaatcaaa aaaaagtata
     4981 atggcgcgtc tcaaaatcac actggaacac cgcgatgaac gttaatgtta tcaaccatcc
     5041 gctcgtccgc cacaaattaa ccctgatgag ggaggcggat tgcagcacct acaaattccg
     5101 gacgcttgcc atcgagctgg cgcgcctgat ggcatacgag gcaagccgtg attttgaaat
     5161 cgaaaaatac cttatcgacg gatggtgcgg tcagattgaa ggcgatcgca tcaagggcaa
     5221 aacattgacc gtcgttccca tcctgcgtgc aggtttgggt atgcttgacg gcgtactcga
     5281 cctgattccg actgccaaaa tcagcgtagt cggactgcag cgcgacgagg aaacgctgaa
     5341 acccgtttcc tacttcgaga aattcgtgga cagcatggac gaacgtcctg ccctgattat
     5401 cgacccgatg ctggcgacgg gcggttcgat ggttgccacc atcgacctgt tgaaagccaa
     5461 gggctgcaaa aacatcaagg cactggtgct ggttgccgcg cccgagggtg tgaaggcggt
     5521 caacgacgcg catcccgacg ttacgattta caccgccgcg ctcgacagcc acttgaacga
     5581 gaacggctac atcatccccg gcttgggcga tgcgggcgac aagattttcg gcacgcgcta
     5641 actgactgat tttcggattt gatatgaatt ttcaagacta tctcgccaca ttttcttcaa
     5701 tcgaccatct gggcggtttg gacgttcagg atgcagatgg caaaacggtt caccacattc
     5761 ctgccgttca gggtaagctc ggttcgctca agttgtacaa cgctttggca gaacgttttg
     5821 acggaaaatt ggacaaagaa gcggcagaac agggtttgat atggtttgcc gaacacgttg
     5881 ccgacgcgcg cgcccatccc ggcaagcatc ccaatatcga cctgcttgaa aaagttgtgc
     5941 aaagcggcga aaccttgctg ctcaagccgc ttgccgcgca ataattttcg accatgccgt
     6001 ctgaaatccg tttcagacgg cattttgtcg gaaagaagac cgtaaaacgg gcattttctt
     6061 ttctatttca ggatacgggc aatgatgttt caacacacag gacgacacat aaagcgttgc
     6121 cctatgtgtt gccctaattt ggaaggggtt acacccctct caaataacat ctgatgctgc
     6181 tgccacgaag gacggatgtc caaatggcgg agtttcaacc attaaggaaa tacgatgaaa
     6241 aaaatgttcc tttctgctgt attgcttctg tcggctgcga cccgaaccgt gtgggcggat
     6301 acggtgtttt cctgtaaaac ggacaacaac aaatatatag aagttcaaaa aatcaaccgc
     6361 aatctttacg aatattcgtt cggcagtgcg gcaaaaaaag aaattgccat acgcaacagc
     6421 aaagctgacc tgttggggcg ttccgacagg tggcaaggta tgggcagcgg tcgttgggca
     6481 acgatgaaat tccaaaacgg cgaatttatg tacaccatat ggacaggctt cgattccgtg
     6541 actcatacgg aaagcagcgg tgtcgttgtg gagcgtaggg gcaaggaagt cgcacgggtc
     6601 ggctgtacgc cgaaaaccgc gcaggcgaat ttcaacgatg acgatttttc ctagtaatcg
     6661 gggcggataa ggcgatggaa acagcgaaac ccgtcatgct gattgtccgt ccgtcgggca
     6721 gggccgcaga agatgtcgaa acttgcctga atgccggttg gcgcgcggaa gtattgagtc
     6781 cggtcgaaat cgaagcagat gctgccggac tggaactttt gtccgaacaa tatgcccgtg
     6841 cggatgccgt gttttgggtc agtccgaccg ccgttgaaac cgccgtcccg taccttaacc
     6901 tttcagacgg cataaagatg cacattgccg tagggaaggg cagccgccgc gcattggaac
     6961 gctgtttggg cagaacggtc atcgcgcctg atgacggcaa cgacagcgag gcggttttgc
     7021 gcctgccggt ttggaacagt ctgcccgaag gtgcgcgcgt attgtttgtg cgcggacacg
     7081 gcgggcgaga ttttctgatg aatgccttgc aggagaaagg tttgcggacg gagatggcag
     7141 aagtctattt cagacggcat aaacctttga actttcaaaa tttccaaacc gaaaatattg
     7201 ccgccgccta tattacgtcg accgagctgg tgcgcttgct gttcgggcag cttccgccgc
     7261 aattttcccg attcttcaaa tccttgctat actttaccca tcatccgcgc attgcggagg
     7321 cattgaagcg cgaaggcgtg tgttcggtcg aaaccgtccc tacgctggaa gccgcgcttt
     7381 cccattcttc catttccgtt tcagacggca tggtctttcc cggaacctca aattaataag
     7441 gagcaaaacg gtgggcgaac ctgaaaacaa atcatccgaa cccgtacgcg agatacaggc
     7501 atcaaaagaa atgccgtctg aaacctcttc cccacgcaaa gaaaacgaaa cagaagtaca
     7561 cattcctgcc gctcctttta tcgtcaaaca gtccggcagc aacgctttgg cagtctgcgc
     7621 cctggtattg gcggcattgg gtttgggtac aagtggtttt ttgtttgtcc aaggacagaa
     7681 tgtcttgaaa aaccaagagc tggcattcaa ccaaaaaatc gacaaagccg ctttgggcga
     7741 gtcggaaaac gccgccctgc tcaaagacaa cctcaaccgg caagccgcca tacaatcaga
     7801 gctcgaccgt ttggacggaa acgtcaaagc aaacggcgaa caaatcttgg aaatgcaaaa
     7861 atcctatcgc gagttgacca aaggacgcgc cgattggctg gtggacgaaa ccgagaccat
     7921 actcaatctg gcggcgcaac agctggtgtt gactggcaat atccaaacgg cagtcggcgt
     7981 attggagcat atcgacagcc gcctgtcccg tttcaatcag gcagagcttc tgccgatcaa
     8041 gcaggcggtc agcagcgact tggcggaact gaaaaaccgt ccctatgtcg atatttccgg
     8101 cacggcattg cgcctcgaca ggctggaaat cgccgtatcc ggactgccgc tgattttgga
     8161 cggcgtattg aaaccgagcg tacaggcacg caacgaagcc gtttccgctt catggtggca
     8221 gaacgtatgg gaaaaatccc tcggcacatt gaaggggctg gtcgaaatcc gccgtttgga
     8281 aaacaacgat gccatgctga tttctcccga acaggcatat tttgtgcgtg aaaacctgcg
     8341 tctccgcctt ttggatgcgc ggactgcatt aatgcagcgc aacagcgaag tctatcaggg
     8401 cgatttgaac aatgccgaaa ccgccgtcag acagtatttt gatgccaaat cccccgccac
     8461 gcagtcgtgg ttgaaggaat tggcggaatt gaaggcgttg gatgtgcgca tgacggcgga
     8521 tgacggtttg aaaaacagcc taaatgccgt ccgcgcctat cgcgacggta cgcgcatgac
     8581 gacggcggaa aatcaagaag cggaacaggc gacttccgaa ccggcaaacg aaaacacagc
     8641 ttccgaaccg gctgccgcat cggatgtgaa gaccatagaa gcaccgtccc tgccttcgga
     8701 acgcaaaccg gaacagcctg caaaaaaaca gcccgtaccg gaaaaggcag ggcgttcgcc
     8761 gtccgctaaa ggagaacgcg catgaaaacg gtagtctgga ttgtcgtcct gtttgccgcc
     8821 gccgtcggac tggcactggc ttcgggcatt tacaccggcg acgtgtatat cgtactcgga
     8881 cagaccatgc tcagaatcaa cctgcacgcc tttgtgttag gttcgctgat tgccgtcgtg
     8941 gtgtggtatt tcctgttcaa attcattatc ggcgtattca atatccccga aaagatgcag
     9001 cgtttcggtt cggcgcgtaa aggccgcaag gccgcgcttg ccttgaacaa ggcgggtttg
     9061 gcgtattttg aagggcgttt tgaaaaggcg gaactagaag cctcacgcgt gttggtcaac
     9121 aaagaggccg gagacaaccg gactttggca ttgatgttgg gcgcacatgc cgccgggcag
     9181 atggaaaaca tcgagctgcg cgaccgttat cttgcggaaa tcgccaaact gccggaaaag
     9241 cagcagcttt cccgttatct tttgttggcg gaatcggcgt tgaaccggcg cgattacgaa
     9301 gcggcggaag ccaatcttca tgcggcggcg aagatgaatg ccaaccttac gcgcctcgtg
     9361 cgtctgcaac ttcgttacgc tttcgacagg ggcgacgcgt tgcaggttct ggcaaaaacc
     9421 gaaaaacttt ccaaggcggg cgcgttgggc aaatcggaaa tggaacggta tcaaaattgg
     9481 gcataccgcc gccagctggc ggatgctgcc gatgccgccg ctttgaaaac ctgcctgaag
     9541 cggattcccg acagcctcaa aaacggggaa ttgagcgtat cggttgcgga aaagtacgaa
     9601 cgtttgggac tgtatgccga tgcggtcaaa tgggtcaaac agcattatcc gcacaaccgc
     9661 cgccccgagc ttttggaagc ctttgtcgaa agcgtgcgct ttttgggcga acgcgaacag
     9721 cagaaagcca tcgattttgc cgatgcttgg ctgaaagaac agcccgataa cgcgcttctg
     9781 ctgatgtatc tcggtcggct cgcctacggc cgcaaacttt ggggcaaggc aaaaggctac
     9841 cttgaagcga gcattgcatt aaagccgagt atttccgcgc gtttggttct ggcaaaggtt
     9901 tttgacgaaa ccggagaacc gcagaaggcg gaggcgcagc gcaacttggt tttggcaagc
     9961 gttgccgagg aaaaccgacc ttccgccgaa acccattgaa accttcagac ggcatcgttg
    10021 aaatgccgtc tgaaaaccat ttcacttgac aaacagactg acagaaaacc cgtatgactc
    10081 ttttaaaaaa cgataccttc ctccgcgccc tgctcaaaca gcccgtcgaa tacacgccga
    10141 tttggatgat gcgccaggcg gggcgttatc tgcccgaata caaagccaca cgcgcgaaag
    10201 cgggcagctt cctcgatttg tgcaaaaaca ccgaattggc gaccgaagtt accatccaac
    10261 ctttggaacg cttcgatttg gacgcggcga ttttgttttc cgacatcctg accgtccccg
    10321 acgcaatggg cttgggactg tattttgccg aaggcgaagg cccgaaattc aaacgcgcca
    10381 tgcaacacga ggccgacatc gccaagctgc acgttcccga tatggaaaaa ctgcaatacg
    10441 ttttcgacgc ggtaacttcc atccgtaaag cattggacgg ccgcgtaccg cttatcggct
    10501 tttccggcag cccgttcaca ctcgcctgtt atatggtcga aggcggcggc agcaaagaat
    10561 tccgcaccat caaaaccatg atgtactcgc gccccgattt gctgcacaaa atcctcgata
    10621 ccaacgccca agccgttacc gcctacctca acgcccaaat cgacgcgggc gcgcaggcgg
    10681 tgcaaatttt cgacacttgg ggcggcgtgt tgagcgacgc ggcgtttaaa gaattcagcc
    10741 tcaaatacat ccgccagatc gtcgccggac tcaaacgcga aagcgaaggc cgccgcgtgc
    10801 ctgttatcgt atttgccaaa ggcggcgggc tgtggctgga aagtatggcc caaatcggcg
    10861 cagacgcatt gggcttggac tggacgtgta acatcggcga agcacgccgc cgcgtcggca
    10921 agcaagtcgc cctgcaaggc aacttcgacc cgttcgccct cttcggtacg ccggaatcca
    10981 tccgcaccga ggtcgcacgt atcctagccg actacggaca cggcagcggc catgtcttca
    11041 acctcggaca cggcatcaac caacacgccg accccgaaca cgccaaaatc ttagtcgata
    11101 ccgtacacga gctgtctcgg cagtatcacg gcgggtaagc cggcaggaaa ccgcccgata
    11161 tgccgtctga agccgagaga tggccggtta gggtaaaaat aaggcaatgc ggcaatatcc
    11221 gccgtgtacg gatagtacat gacggcggcg ttgtcgtatt ggcgcaatcc caaccgtccc
    11281 tatgttcaga cggcattttt gttttcagat gcagggaaaa ccgatggcaa aaacgcttaa
    11341 aaccctttac caatgcaccg aatgcggcgg cacttcgccg aaatggcaag gcaaatgccc
    11401 gcattgcggc gagtggaaca cgcttcaaga aagcttagcc gcgcccgagc cgaaaaacgc
    11461 ccgcttccaa tcttgggcgg cggatacctc gaccgtccaa tccctctccg ccgttaccgc
    11521 tgccgaagtg ccgcgcaacc cgaccggcat gagcgaactc gaccgcgtat tgggcggcgg
    11581 tttggtcgat ggcgcggtca tcctactcgg cggcgatccc ggcatcggca aatccacgct
    11641 gttgttgcaa accatcgcca aaatggcgca aagccgcaaa gtgctgtacg tttccggcga
    11701 ggaatccgcc caacaagtcg ccctgcgcgc gcagcgtttg gaactgccca ccgaaggcgt
    11761 gaacctgctt gccgaaatcc gcatggaagc cattcagacg gccttgaaac agcatcagcc
    11821 cgaagtcgtg gttatcgatt ccatccaaac catgtattcc gaccaaatca cttccgcccc
    11881 cggctccgtg tcgcaggtgc gcgagtgtgc agcccaactg acgcgcatgg cgaaacagat
    11941 gggcatcgcc atgatactgg tcggacacgt gaccaaagac ggcgcgattg ccggcccgcg
    12001 cgtgctggaa cacatggttg ataccgtgct gtatttcgag ggcgaccaac attccaacta
    12061 ccgcatgata cgcgccatca aaaaccgctt cggcgcggca aacgaactgg gcgtgttcgc
    12121 gatgacggaa aacggtttga aaggtgtgtc caacccgtcc gccatcttcc tcgccagcta
    12181 ccgcgacgat acgcccggct cgtgcgtttt ggttacacag gaaggcagcc gcccgctttt
    12241 ggtcgaaatt caggcattgg tcgatgacgc gcacggcttc acgcccaaac gcctcaccgt
    12301 cggactggaa caaaaccgtc ttgcgatgct gcttgccgtg ttaaaccgac acggcggcat
    12361 cgcctgtttc gatcaggatg tgttcctcaa cgccgtcggc ggcgtgaaaa tcggcgaacc
    12421 ggcggcggat ttggcggtca tcctcgcgat gctttccagc ttccgcaacc gccctatgcc
    12481 tgaaaaaacc gtggttttcg gcgaaatcgg cttaagcggc gaagtccgcc ccgtcgcacg
    12541 cgggcaagag cggctcaaag aagcggaaaa actcggcttc aaacgcgcca tcgttcccaa
    12601 agccaatatg ccgcgcaacg ccaaagagtt tccgaacctg aaaatctacg gcgtttcgag
    12661 tttgcaggaa gccatcgata tttgccgtga cagcagggaa taaacggaaa tgccgtctga
    12721 aatcaggttt cagacggcat ttggtttgtg gcggattgaa acaagaaggc ataccggcga
    12781 cagataagat ttgtggcaaa gttgcctgtg atgtggcaaa aacacacacg cccgtcatcc
    12841 ccgcgagggc gggaatccgg aatcgtccgt ttcggcaatg attgaaaatc acggcaatcc
    12901 aaccgcttgg attcccgact tcgcgggaat gaggggcgtg tgcgtttgat ttccatccgc
    12961 catatgtcgg caacgggctt attcgcctat ggttttttgt atcagttttt cggcgtttgc
    13021 caaagtgttt gccacttcgt cgaaaccgat gcggctgccg gcgatgaggg cgcgcgtatc
    13081 ggtataggcg gcgcgtactt tgccgtccgt ttcggtaacg aggacgcgca ggggcagttg
    13141 cagggcgaag gcggggtctt tgaccatcag cggcgtaccg gctttgggcg tgccgaagac
    13201 gatgactttt gccggctgca tcgttaagcc gtttcggcgg gcggcttcct gatggtcgat
    13261 gacggcaaaa atgtccatcc ctttgctttt tatggcggtt tcaaggcggc tgacggtttc
    13321 gtcaaaactg tattttgagg tgagggtatg cgtggtcata gcggtttcgt tttgggtgga
    13381 cggttcgctg gcaggatgtg ccgaagcggt tgaaatgcag agtgcggatg cggcaatcag
    13441 ggggagtatg tgtttcatcg tatttccttt ttccttttcg gtttgtttat caggcttcgg
    13501 acggggcggc acgcgccgcg cccgatgttg cgccgcgtgc cggaacgccg tatgccgtct
    13561 gaaagcctgt cctttcagac ggcattgcgt catttcatcc cttttttgag caggtcttca
    13621 taaccgccgt gattggcaac atttgtataa cctgcttttt tcagctcttg aagggcggct
    13681 tcggcacgcc gtccgctgcg gcagtagagg ttgaccggcg tgtctttgtc gggcgcggct
    13741 tcgtgtatgc ggcggacgat ttggtcgacg gggatgttga ccgcgttgtg caaatgccct
    13801 tcgctaaatt cctgttcgga acggacatcg atccaaacgg ccggatgttg cgcggtttgg
    13861 gcggcggata cgggtttttg cggggctgcc tgcgcggcaa aggcggctga ggcaatgagt
    13921 gcggcggtaa tcaggtgttt gatattcata gggttctcct gcggttgtcg ttttaaggga
    13981 cgggaagtta ttttatctgt tccaaagcgg cggcatctat gtcccaacgc caaacgccgc
    14041 cgcctttgcc gtctgtgccg cgcaaaaaca ggtagcggac ggaaacggcg gcgggcggtt
    14101 gtccgcgcag tttgaagtag cgtgcggcgg caacggcgta aatcagcgcc tgaaggtaat
    14161 agtggtggtg cgtgacggct tcgtccatcg cctgttgcgt gtaggcggat gcgtccgcgc
    14221 cgagatggtt tgatttgtag tcgatgatgc agatattgcc gtcggggtct tggcagacca
    14281 tatcgataaa gccgtttaaa aagccgttga cggtgtggaa gtcgagcgtt tcggcggcgg
    14341 cacggcagac ttcgggcagc ctgatgtcgt cgcgggcaaa ccagtcgcgc agccgtttga
    14401 ggccgaagtc ttcggtatgg agggtaaagc ccatttcggg acagcggcac tcgggcggaa
    14461 tgtcggacag gcagtatgcc cccgtcagcg gcgttttacg gcaggcttcc gccatttcgg
    14521 caacggcggg cagccatatt tcttcaaaac cgtatttttt cagcttgtcg gcaatgaggg
    14581 tttcctgtcc ggcggctgct tgtccgaatt tgaaatcttc aagaatttcg tgcaggcaca
    14641 gccccgcctg cgtgcctttc ggaaaatcgt gtatcgatat gccgtctgaa gccgtcggcg
    14701 tttcagacgg catcgccggc accgaggttt cggcggcatc caaggacggg caggcatctt
    14761 cttcgccgcc gtcgggcgtt tgggtatggc ggcttaaggc ggtaaagcta gtgtggcgga
    14821 caaatcggaa tccgcgttcg ggaatgctgt ttgcggcaaa ttcggcggtt tgaccggcgt
    14881 tgctgcgata ggcggcaggg ggcggcgcat tttcggtgaa ggcgaaattt gtgccggaag
    14941 gggcgttgtc cgccacgcgc cgccagttgc gtttgagcat cgcgatgccg tctttttcac
    15001 acgcataggc acggcggacg gtttcacggc tgtcttgggg cgagccttca atcaggtagg
    15061 cgagggggtt gtcggcagta ttggtggagt acgcggcgta gatgttgagc tgttcctcgg
    15121 cacgcgtcag tgcgacataa agcaggcgca ggcgttccgc catttcttca tcggcgtatt
    15181 gtttctgttc gtcttccgac agttgcgcct ttgccaacag ttcggttcgg tttgcgcttt
    15241 ggtggaggat ttgccagtcg gacggcccgg tatcttgcgc gtcccacgca aacgggcagt
    15301 acaccagcgg atactgcaaa cctttcgagg cgtgcatggt aacgattttg accaaatctt
    15361 cgtcgctttc cagacggatg gcgcggttgt cgccgccgtt gttgccggcc aggctgattt
    15421 ggtcgcgcag ccatttgtgc agcgcggcgg ggttgcggtt ttgcgcgtct tcggcggcaa
    15481 gcagttcgag cagttggaaa tagttggtca ggctgcgccc gttgttccgg cttaagaggc
    15541 gtgtttcgat gccgtgtgtt tgggaaaatt gctgcatagc ggcgaaaatg ccgtattgat
    15601 tccagttgtc gagcgcggtt cgggcagatt ccgcccaatg caaaatctcg ctttcgtttt
    15661 ggttgaagtc gtgcaattgc tgcgcgttat agccgaatat gctgcttgtc aggacaaagc
    15721 gcagcgttcc ggcgcggcgc ggttcgagcc agaagccgat gagtgcggac agggcggcgg
    15781 cttcgggcga ggcgaacacg gattcgcgcg aaagcaggac gctttgcacc tgccgttttt
    15841 tcagggcggc ggaaaccatc accgcctcgt tgtgcgtgcg taccagcacg gcaatatcgc
    15901 ccgactgcaa cgggcagcct ttgaaattca gacggcctcc ggcggcttcg ttgagcgcgt
    15961 gggcgatttc gtcggcgcaa tagtcggcgg cacgtcggcg caaaacgtct ttgttggctt
    16021 tttcattgtc gttttcgtgc agccaacgaa cctgtacggc aggacgtttg ggcgacaatc
    16081 tgctttcggc acgcgccgca ccgacttccg aatagccgat gttttccaaa acgaacgggc
    16141 gttctttgag gcggaacagt gcgcctatgc tgccgataag cgcggcgtgg ctgcggtagt
    16201 tggtggcgag cgtgtagcgg tgccgcgcgt cttccgctgc ctgaaggtag gcgtaaatgt
    16261 ccgctccgcg aaagctgtaa atcgcctgtt tgggatcgcc gacgaggaac agcggtcggt
    16321 tttgggcgat gaagattttt tggaagattt cgtattgcag cgggtcggtg tcttggaact
    16381 cgtcgatcag cgcggtttcc cagttttcgg caacggcgcg ggcgagggtt tcggcgtgcg
    16441 gattgtcggt cagcgcggtg tggacatcga gcagcaggtc gtcgaaaccg cgttcgcggc
    16501 gcgatttttt catctcggca aggctgcggt tgaggtatcc gattaaatcc agttgcagcc
    16561 ggattattgt tgcttcttcc gcttcttcga gtgcgtccaa atcacgcctg aagtctgcca
    16621 gtttctgcaa ttcggcaaat actgccgcat cgggcgtttt gccttttttc agtccggctt
    16681 cgagtttgtc ggacgcaagt ttcaagagcc gttcgtgcag ctttgcctcc aaaaagggca
    16741 aatccggcga cagggctttt tgttccaatg cgtcaaacag ttcggcaaaa ctgtttttcc
    16801 gatatgaatt gccgtttaaa aggggatgga tgcgccaaaa agtttctttt aattcagcca
    16861 gatttccgca aatgttttgc cagcttgcca aagcggcatc ccgggcttgc ggcaaatcgg
    16921 tttgcggacg gcggaaattc aggtacgggc gggacaggta ggcgcggatt tgggcaagga
    16981 cggtttgcgg cacagctttg cgtttaaacg ccaatgcggc aagcaccgga tcattgctga
    17041 cgcgttcccg ccaaaaatct tgcgccggga caagcaggcg gtcgccgtct tcttcggtca
    17101 gttcgacatc gaacggtgct tggcacaaaa acgcgtagtc gcgcaggatg cgctggcaga
    17161 agccgtggat ggtatagatg gcggcgttgt cgaattgccc gatggcggct ttgaggcgga
    17221 caatcagacg cgtccggccc tctttttgca aagcctgttt taagagtgcg ggcaggaagg
    17281 tgtcgccttt gtggtgttcg gcgcagtagg cggcgatgcc gtctgaaagc gtgtcgtctc
    17341 caagtttggc aatttcttta ctttctaaaa cctgcaacac atcgtccaaa cgtgcgcgca
    17401 ggcgtgtttt caactcggcg gtggcggctt tggtgaaggt aacgaccaat acgcgctcga
    17461 cgtttttttg ttccaatacg atcaggcgcg taaacagggc ggcaatgccg taggtttttc
    17521 cggtgccggc agaggcctca atcaggttgg tgccggcaat ggggacggtc agcgggtcaa
    17581 atgcttggat gggtgcagac atagtgcgcg ctcggaaaac ggttggacgg tatagtggat
    17641 taacaaaaat caggat
//
LOCUS       DAXHSF010000023        17251 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.023, whole genome shotgun sequence.
ACCESSION   DAXHSF010000023 DAXHSF010000000
VERSION     DAXHSF010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17251
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.023"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(<1..200)
                     /locus_tag="ACFM8S_000637"
     CDS             complement(<1..200)
                     /locus_tag="ACFM8S_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313744.1"
                     /translation="MPSERLSDGIFPGVLNRIHETLDAFGGNVGCRAMFGGDQRTKYA
                     GKGLMPSEGSDGIGVGESEAVA"
     gene            complement(218..775)
                     /locus_tag="ACFM8S_000638"
     CDS             complement(218..775)
                     /locus_tag="ACFM8S_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFC6313745.1"
                     /translation="MDALKLLTNRRSSKKLKHPAPDAAELEQIFQAATQVPDHGNMRP
                     FRFTVIQGEVGLQRFRDVLKQTVAELNFGDDAMKKAEKVGNMAPMVIGVTFAPNRDVP
                     KPKPEWEQMLTAGCAAYALQLAATAQGFDNVWITGMWVNSPLLREAFGCADKDKIIGL
                     MMVGTPTEEVHKPKNTDLEAFVSHW"
     gene            complement(851..2485)
                     /locus_tag="ACFM8S_000639"
     CDS             complement(851..2485)
                     /locus_tag="ACFM8S_000639"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:1900727 - osmoregulated periplasmic glucan
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFC6313746.1"
                     /translation="MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELHPFNGT
                     GADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKS
                     MLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAA
                     VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLD
                     MAVVQNRPAGSLRRFVVLVVGETTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSC
                     GTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKV
                     PNTDVTSLNLPEYCRNGECLDNILLTKFDEVLNKNDKDAVLILHTIGSHGPTYYERYT
                     EAERKFTPTCDTNEINKCTRATLVNTYDNTVLYVDQFIDKVIRKLENRDDLESVVHYV
                     SDHGESLGENGMYLHAAPYAIAPSGQTHIPMVMWFSKAFRQHGGIDFQCLKQKAAENE
                     YSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR"
     gene            complement(2887..3993)
                     /gene="serC"
                     /locus_tag="ACFM8S_000640"
     CDS             complement(2887..3993)
                     /gene="serC"
                     /locus_tag="ACFM8S_000640"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HFC6313747.1"
                     /translation="MSLYPIYNFSAGPAVLPEAVLETARQEMLDYNGTGFPVMAMSHR
                     SEMFLSILHHAEQDLRQLLKVPDNYKILFLQGGATTQFNMAAMNLAHGFRTADAVVTG
                     NWSRIAYEQMSRLTDTEIRLAAHGGEQFDYLDLPPVETWDVAPDSAFVHFAVNETVNG
                     LQYREVPRLSEGMPPLVCDMSSEILSREFDVADYGLIYAGAQKNIGPAGVTVVIVRED
                     LLERCPNDIPDVFNYRSHLNREGMYNTPSTYAIYMSGLVFRWLQAQGGVKKIEAVNRL
                     KAQTLYETIDGSGGFYINRIRPNARSKMNVVFQTGDEELDRRFVLEAELQGLCLLKGY
                     KTVGGMRASIYNAMPLEGVRALADFMRDFQRRYG"
     gene            4358..4789
                     /gene="rimP"
                     /locus_tag="ACFM8S_000641"
     CDS             4358..4789
                     /gene="rimP"
                     /locus_tag="ACFM8S_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFC6313748.1"
                     /translation="MDIQTILEKTLPGLGYELVDFELTAQGTLRVFIDKEGGITVEDC
                     ATVSNHLSRVFMVEDIDYKNLEISSPGLDRPLKKAADFVRFAGQNAKIKTRLPIDGQK
                     NFIGKIEGCENDTVTVSFDGKTVQIELGNIDKARLRPEFKF"
     gene            4817..6319
                     /gene="nusA"
                     /locus_tag="ACFM8S_000642"
     CDS             4817..6319
                     /gene="nusA"
                     /locus_tag="ACFM8S_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFC6313749.1"
                     /translation="MSREMLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHM
                     DVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQEEIPGTTIQIGEYYEEQL
                     PNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVV
                     AGKLDALIPRDQMIPRENFRSGDRIRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYA
                     NEVPEIADGMLEIRAVARDPGQRAKVAVKANDQRIDPQGTCIGVRGSRVNAVSNELSG
                     ERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVAEDQLALAIGRGGQNVR
                     LASDLTGWQLNIMTSAEADERNAAEDAAIRRLFMDHLNVDEETADVLVQEGFATLEEV
                     AYVPAAELLAIEGFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGIDADM
                     LRSLAEAGITTRDDLAELAVDELIEITGVNEETAKAVILTAREHWFTEDK"
     gene            6332..9220
                     /gene="infB"
                     /locus_tag="ACFM8S_000643"
     CDS             6332..9220
                     /gene="infB"
                     /locus_tag="ACFM8S_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFC6313750.1"
                     /translation="MSNTTVEQFAAELKRPVEDLLKQLKEAGVSKNSGSDSLTLDDKQ
                     LLNAYLTKKNGSNGGTISIRRTKTEVSTVDGVKVETRKRGRTVNIPSAEELAAQVKAA
                     QTQAAPVRPEQTAEDAAKARAEAAARAEARAKAEAEAAKLKAAKAGNKAKPAAQKPTE
                     AKAETAPVAAETKPAEESKAEKAQADKIPSKKPAEPKEKAAKPKHERNGKGKDAKKPA
                     KPAAPAAPQPVVSAEEQAQRDEEARRAAALRAHQEALLKEKQERQARREAMKQQAEQQ
                     AKAAQEAKTGRQRPAKPAEKPQAAAPAVENKPVNPAKAKKEDRRNRDDEGQGRNAKGK
                     GGKGGRDRNNARNGDDERVRGGKKGKKLKLEPNQHAFQAPTEPVVHEVLVPETITVAD
                     LAHKMAVKGVEVVKALMKMGMMVTINQSIDQDTALIVVEELGHIGKPAAADDPEAFLD
                     EGAEAVEAEALPRPPVVTVMGHVDHGKTSLLDYIRRTKVVQGEAGGITQHIGAYHVET
                     PRGVITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTIEAIAHAKAAGVPM
                     VVAVNKIDKEAANPERIRQELTAHEVVPDEWGGDVQFIDVSAKKGTNIDALLEAVLLE
                     AEVLELTAPVDAPAKGIIVEARLDKGRGAVATLLVQSGTLKKGDMLLAGTAFGKIRAM
                     VDENGKSITEAGPSIPVEILGLSDVPNAGEDAMVLADEKKAREIALFRQGKYRDVRLA
                     KQQAAKLENMFNNMGETQAQSLSVIIKADVQGSYEALAGSLKKLSTDEVKVNVLHSGV
                     GGITESDVNLAIASGAFIIGFNVRADASSRKLAENENVEIRYYNIIYDAIDDVKAAMN
                     GMLSPEEKEQVTGTVEIRQVISVSKVGNIAGCMVTDGVVKRDSHVRLIRNNVVIHTGE
                     LASLKRYKDDVKEVRMGFECGLMIKGYNEIMEGDQLECFDIVEVARTL"
     gene            complement(9381..10721)
                     /locus_tag="ACFM8S_000644"
     CDS             complement(9381..10721)
                     /locus_tag="ACFM8S_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase/DUF3482 domain-containing protein"
                     /protein_id="HFC6313751.1"
                     /translation="MNKQPLSLAVVGHTNTGKTSLLRTLLRDSGFGEVKNAPSTTRHV
                     EEAAISDGADTLVFLYDTPGLEDAGGVLEWLENHTDNRSDGIERLQQFLGSHGAHHDF
                     NQEAKVLRQVLQSDMAMYVIDAREPVLDKYRDELTILSWCAKPVMPVFNFTGGQPPES
                     WTTMLARRNLHVFAGFDTVAFDFEGELRLWENLATMLPERSTLDRLTAMRRREWQRLD
                     GEARREIADFLIDAAAFRQEVDENEDTATVLQTMQAEIRQLERQMQQRLFALYRFYHS
                     EIDGGDWMPQAFRQDPFDSELLKQYGIRTGTGAATGALIGLGLDIATLGGSLGLGTAI
                     GGFLGGILPNTRTISDKLAGRQTLHTDPETLTLLAARALDLLHVLQTRGHAAQSDIEL
                     HSRKAPWDAARLPPELNKARSHWKWSSLNTHRPETSHAERREYAEKLGIRLAGK"
     gene            complement(10795..12135)
                     /locus_tag="ACFM8S_000645"
     CDS             complement(10795..12135)
                     /locus_tag="ACFM8S_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HFC6313752.1"
                     /translation="MLNPSRKLVELIRILDEGGFIFSGDPVQATEALRRVDGSTEEKI
                     IRRAEMIDRNRMLRDTLERVRAGSFWLWVAAATFAFFTGFSGTYLLMDNQGLNFFLVL
                     AGVLGMNTLMLAVWLAMLFLRVKVGRFFSSPATWFRGKDPVNQAVLRLYADEWRQPSV
                     RWKIGATSHSLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLGDSSSVRLVEMLAWLPA
                     KLGFPVPDARAVIEGRLNGNIADARAWSGLLVGSIACYGILPRLLAWAVCKILLKTSE
                     NGLDLEKPYYQAVIRRWQNKITDADTRRETVSAVSPKIVLNDAPKWAVMLETEWQDGE
                     WFEGRLAQEWLDKGVAANREQVAALETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLS
                     EAAQGGAVVQLLAEQGLSDDLSEKLEHWRNALTECGAAWLEPDRAAQEGRLKDQ"
     gene            12278..12904
                     /locus_tag="ACFM8S_000646"
     CDS             12278..12904
                     /locus_tag="ACFM8S_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFC6313753.1"
                     /translation="MYTGERFNTYSHLSGLILAAAGLALMLLKTIGHGDGYRIFSVSV
                     YGISLLLLYLSSSLYHGIAAGKLKSILKKTDHCMIYVLIAGSYTPFALVSLRNGPGWT
                     VFSLSWLLAAAGIAQELTIGRKSEKRLLSIVIYVVMGWMVLAVMKSLTASLPSAGLAW
                     LAAGGMLYSVGIYWFVNDEKIRHGHGIWHLFVLGGSITQFVSVYGYVI"
     gene            complement(12968..14386)
                     /locus_tag="ACFM8S_000647"
     CDS             complement(12968..14386)
                     /locus_tag="ACFM8S_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC6313754.1"
                     /translation="MENILSVLVGTVNRFLWDYLLIYALLGIGLFFTLYLGAPQITKL
                     GAGFKSVFGGLFAKGDKDDKSLSQFQALAVAISAQIGTGNVAGVATAITAGGPGAIFW
                     MWVSAVLGMSTIFAEALLAQKYRVVSHGKYIGGPAFYITHGLTPKIGRGAARFLSGFF
                     SIALIIALGFIGNATQANSIASAVTIAFDVPSLAVGIVLAVLAGMVVIGGVNRIANIA
                     QFVVPFMAVVYILCAVVILFEFSDYIVPMFNHIFTAAFNPEAVLGGAAGIGMREAIRF
                     GVARGLFSNEAGMGSTPHAHATADVKHPVQQGMAAFVGVFIDTILVCTATALIILLTD
                     ANLSGEQGAAVTQFAFNKAFPGFGSQLLAVCLTFFALTTIIGWYYFGESNIRFLFRGR
                     HLGIYRALVLLAIVLGTLGKVDLVWSLSDMFNGFMVIPNLIALFLLRKEIRAIYDDYL
                     AQKKAGRDLSYQYEFHEFHDKG"
     gene            14713..15441
                     /locus_tag="ACFM8S_000648"
     CDS             14713..15441
                     /locus_tag="ACFM8S_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFC6313755.1"
                     /translation="MAGHSKWANIQHKKARQDAKRGKIFTRLIKEITVAARMGGGDPG
                     SNPRLRLALEKAAENNMPKDNVQRAIDKGTGNLEGVEYIELRYEGYGIGGAALMVDCL
                     TDNKTRTVADVRHAFTKNGGNLGTDGCVAFNFVHQGYLVFEPGVDEDALMEAALEAGA
                     EDVVTNDDGSIEVITAPNDWAGVKSALEAAGYKSVDGDVTMRAQNETELSGDDAVKMQ
                     KLIDALEDLDDVQDVYTSAVLNLD"
     gene            complement(15500..15988)
                     /locus_tag="ACFM8S_000649"
     CDS             complement(15500..15988)
                     /locus_tag="ACFM8S_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HFC6313756.1"
                     /translation="MTPLFRKAVWLLFAVSVCAFAGSLAAQYVLGMEPCVLCISQRLC
                     VLATALCTAIVLMCRPRRRAGGLFGAVFISIPAVTGISVAAYQLWLQSLPPGTAPSCG
                     APWTFRLKGWPLFDWFEPVVRGFGNCAEPDYLLGVALPVWSVAYFLAVALTVWWAWAR
                     AK"
     gene            complement(16747..17211)
                     /locus_tag="ACFM8S_000650"
     CDS             complement(16747..17211)
                     /locus_tag="ACFM8S_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HFC6313757.1"
                     /translation="MKELDKIDFRILKILQQNARIPMMELAEKVGLSTTPVTERVRRL
                     EREHYISGYHAHLNPHLLGKPLLVFVELKLQSKSGNIFEDFKKEVLKIPQIMECHLVS
                     GEYDYLIKVRLPDMSAYRDMLGNILLQLPAASESRSYVVMEEVKENPVLDLD"
BASE COUNT         4544 a         4828 c         4337 g         3542 t
ORIGIN      
        1 tgcgctaccg cttctgattc cccgacaccg atgccgtctg aaccttcaga cggcatgaga
       61 cctttgcctg cgtacttggt gcgctggtcg cctccgaaca tggcgcgaca cccgacattt
      121 ccgccgaacg catcgagcgt ttcatgaatc cggtttaaaa cgcctggaaa aatgccgtct
      181 gaaagccttt cagacggcat ttcgcttgag attccgttta ccaatggctg acaaacgctt
      241 ccaaatcggt attcttgggc ttatgcactt cctctgtcgg cgtgccgacc atcatcagcc
      301 cgatgatttt atccttatcc gcacatccga aagcctcacg caacaagggg ctgttgaccc
      361 acatccccgt tatccagaca ttgtcgaaac cttgcgccgt tgccgccagt tgcagcgcat
      421 acgccgcaca acccgccgtc agcatctgct cccattccgg tttcggctta ggcacatcgc
      481 ggttcggcgc aaacgttaca ccaatgacca tcggcgccat attgcccact ttttccgcct
      541 ttttcatcgc atcgtcgccg aaattcaatt cggcaaccgt ttgcttcaac acatcgcgaa
      601 aacgttgcaa tcctacctcg ccttgaatca cggtaaaacg gaaggggcgc atattgccgt
      661 gatcgggaac ttgggttgcc gcctgaaata tttgttccaa ctccgccgca tcgggggcgg
      721 ggtgcttcag ctttttggaa gatcggcggt tcgtcaataa ttttaaagca tccatatcgt
      781 tatactccgg tcatcggtcg ggtgttcgga cacatatgcc gtccgaaggc ttcagacggc
      841 atatccggca tcagcgcgga cggcggcagg ctgccaatat atccatttcc ttccgatagg
      901 tttgactgtt ggaaatgtcc atcagcccca ataccgtgct gaaatagtgg tcgtgcgaat
      961 attcgttttc cgccgctttt tgtttgaggc attggaaatc tatgcccccg tgttggcgga
     1021 aggctttgga aaaccacata accatcggga tatgcgtctg cccggaaggc gcgatggcgt
     1081 aaggcgcggc gtgcaggtac atcccgtttt cgcccaaact ttcgccgtgg tcggaaacat
     1141 aatgcaccac gctttccaaa tcgtcgcggt tttcaagttt gcggataacc ttgtcgataa
     1201 actggtccac atacaaaacc gtattgtcgt aagtgttgac cagcgtggcg cgggtgcatt
     1261 tgttgatttc gttggtgtcg caggtcggcg tgaatttgcg ttcggcttcg gtatagcgtt
     1321 cgtaatacgt cggcccgtgg ctgccgatgg tatgcaggat taaaaccgcg tctttatcgt
     1381 ttttgttgag gacttcgtcg aacttagtca gcaggatatt gtcgaggcac tcgccgttgc
     1441 ggcagtattc gggcaggttg agcgaggtaa cgtcggtatt cggcactttg ccgcacacgc
     1501 ccttgcagcc ggaatcgttt tccaaccagg taacttccac gccggcgcgc tgcacgatgt
     1561 ccagcaggtt gtcttggtgt tcggctttga tttcgtcata atccgtgcgg tcgaaagttg
     1621 agaacataca cggcagggag tgcgcggtcg atgtaccgca gcttctgacc tgcgggaaat
     1681 tgacaatttc atcgccgcgc gcggcaagca gcggcgtagt ttggcggctg taaccgttca
     1741 aaccccagtt ggcggcacgc gtggtctcgc ccacgaccag caccacgaaa cggcgcaggc
     1801 tgccggccgg ccggttttgc acgaccgcca tatccaattg cgtataagga atattggaac
     1861 gcttccaatc tttgtatttc gacacgcccg cgccgatgaa attagacggc acaatcagat
     1921 gggttactga tttattgttg cggaaaaacg aggcgtaatc ctgatattgc aacattgcga
     1981 tacccaacgc gcacaaaaag gaaacggcgg caagcacaag gcgcgtcaaa agctccttat
     2041 accaaacgcg gtatttaacc ttgacggcga tatacgccag cgcgggcaat acgcccaaac
     2101 atacaatcca cagcacatag cccggcgtaa tcaggcgcgc gctttcggca gccgtagttt
     2161 gcaagacatt attcaacatc gacttgttga aatagatatt gaaaaatatt tcttggtaag
     2221 acaccgccgc actgataacc aatatcaacg gaatcaatac cttatgcacg aaaggcaggg
     2281 caatgacgtg aaaaacgaaa ttacttaaaa aaaacagcac caccggcatc gtatagagga
     2341 agatatccgc cccggtgccg ttaaaaggat gaagctcgac aactttggca aaaaaggcgt
     2401 aattcaatac cagcgaggaa tacagggaaa ggaaggcaat cagcgcggaa gagccgagct
     2461 tcggcctcag gtttggtttt atcatttgga atgtgtcgga taagggttgg aaaaggcatc
     2521 cggcatttgg aatccggatt attgaaaaag attcttaatt ataaggcaac ggagcaaagc
     2581 agggcaagaa aacggcggct gtgcgggggt tccgcctgcc attcaaacgt ccggcagaca
     2641 taaaaacatc gtaagcaaga ttcgaaccgg tctgcaaccg cccctgccag aaaaacgggc
     2701 aaagcatttc atattggaaa aacccgaccg cgccgccgac gggacagtcc ggcacaaaca
     2761 gcatcacgct cggattgaaa aggacggata gccgccggca gcacaatctt accacctcca
     2821 aaacgccgcc ggagaacgcc gaaacagccg atgccgtctg aagccgcttc agacaacatc
     2881 gggacatcaa ccgtaacgcc gttggaaatc gcgcataaaa tccgccaaag cccgcacgcc
     2941 ttcaagcggc atcgcattat aaatgctggc acgcataccg ccgacggttt tatagccctt
     3001 aagcaggcac aagccctgca attcggcttc cagcacaaaa cggcggtcaa gctcctcatc
     3061 ccccgtttgg aacacgacat tcattttaga acgcgcattc ggacggatac ggttgatata
     3121 gaaaccgccg ctgccatcta tcgtctcata caaggtttgc gccttcagcc gattgaccgc
     3181 ttcaattttt ttcacaccgc cctgcgcctg cagccagcgg aacaccagcc ccgacatata
     3241 aatcgcgtaa gttgacggcg tgttgtacat accttcgcgg ttcaaatgcg aacggtagtt
     3301 gaacacatcg ggaatatcgt tcggacaacg ctcgagcaaa tcctcacgca caatcaccac
     3361 cgtaacccct gccggcccga tgtttttctg tgcgcctgcg taaatcagtc cgtagtcggc
     3421 aacatcaaac tcgcgcgaca aaatctcgct ggacatatcg cacaccagcg gcggcatgcc
     3481 ttctgaaagg cgcggcactt cacggtattg cagcccgttg accgtttcat tgacggcaaa
     3541 atggacaaac gccgaatcgg gtgcaacatc ccacgtttcc acaggcggca ggtcgagata
     3601 gtcgaactgc tcgccgccat gcgccgccaa acggatttcc gtatcggtca aacggctcat
     3661 ctgttcataa gcgatacggc tccagttgcc cgttaccacc gcgtcggcag tgcggaaacc
     3721 gtgtgccaga ttcatggctg ccatattaaa ttgggttgtt gctccgccct gcagaaacaa
     3781 tatcttatag ttgtcaggca ctttcaaaag ctgcctcaaa tcctgttccg catgatgcag
     3841 gatgctcaaa aacatttccg aacggtggct catcgccatc acgggaaaac ccgtaccgtt
     3901 gtagtccaac atttcctgcc gcgccgtttc caacacggct tcgggcaata cggcagggcc
     3961 ggcggaaaaa ttgtaaatcg gataaagaga catgatgcag ccttgattct gaacaataac
     4021 ccgcctgatt ttaggcttgc agcaggcttt gtgcaaggat gggaaatacc tgtcctccgc
     4081 ccgattccat gccgcccgaa cacggaaaac aacatcaata tattgattta caaatataaa
     4141 aatcatgcta gcgacaaata gatacatttg ttttgtcaac aatattcacg atttcccatt
     4201 acaaacctcc cttacacccg cttttttccg tcccaaaaac acaaaataaa tcaacacttt
     4261 catttctccg caaaagcggt tataatcacg ccgatttttc aactttgacg aaaaatggcc
     4321 acggggccat ttttttatta tgtacatagg gagcagcatg gatatccaaa ccatcctcga
     4381 aaaaaccctg cccggcctgg gctacgaact ggtcgatttt gaactgaccg cgcaaggaac
     4441 attgcgcgtg ttcatcgaca aagaaggcgg cattaccgtc gaagactgcg caaccgtcag
     4501 caaccacttg agccgcgtct tcatggttga agacatcgac tacaaaaacc tggaaatttc
     4561 cagccccgga ctcgaccgcc ccttgaaaaa agccgccgac ttcgtgcgct ttgccggtca
     4621 gaatgccaaa atcaaaaccc gcctgccgat agacggtcaa aaaaacttta tcggtaaaat
     4681 cgaaggctgc gaaaacgaca ctgttaccgt ttccttcgac ggcaaaaccg tacaaatcga
     4741 attgggcaac atcgacaaag cccgcctgcg ccccgaattc aaattctaaa acacaacaat
     4801 attggagatg ttcaaaatga gtcgtgaaat gttacagctg gcagaagcac tggcaagcga
     4861 aaaaaacgtt gatgcggaag tcgtcttcca agcactggaa ttcgccctgt ctaccgccgc
     4921 caagaaaaag gcagaccgcg aacacatgga tgtccgcgta caaatcaacc gcgacaccgg
     4981 cgaataccaa accttccgcc gctggctgat tgtcgccgat gaagactata cctatcccga
     5041 tgtcgaaaaa accatcgagg aaatccaaga ggaaattccc ggcactacca tccaaatcgg
     5101 cgaatactac gaagagcagc tgcccaacga aggcttcggc cgccaagccg cgcaaaccgc
     5161 caaacaaatc atcctgcaac gcatccgcga tgccgagcgc gagcagaatc tgaacgagtt
     5221 tctcgccgtc aaagaagaca tcgtgtccgg cacggtcaaa cgcgtcgaac gccacggcat
     5281 catcgtcgaa gtcgttgccg gcaaactgga cgcgctgatt ccgcgcgacc aaatgattcc
     5341 gcgcgaaaac ttccgcagcg gcgaccgcat ccgcgccctc ttcctgcgcg tcgaagaaat
     5401 cggcaacacc ggccgcaaac aagtcatcct gagccgtact tccggcgatt tcctcgtcaa
     5461 actgtacgcc aatgaagtac ctgaaattgc agacggcatg cttgaaatcc gcgctgtcgc
     5521 ccgcgacccg ggccagcgtg ccaaagtcgc cgtcaaagcc aacgaccagc gcatcgatcc
     5581 gcaaggcacc tgtatcggcg ttcgcggttc gcgtgtcaat gccgtcagca acgaattgtc
     5641 cggcgagcgc atcgacgttg tcctttggtc gcccgagcct gcccaattcg tcatgagcgc
     5701 gctctcaccc gccgaagtca gccgcatcgt catcgacgaa gacaaacacg ccgtcgatgt
     5761 catcgttgcc gaagaccagc tcgcgctcgc catcggacgc ggcggtcaaa acgtgcgcct
     5821 tgcttccgac ctgaccggct ggcagctcaa catcatgact tccgccgagg cagacgaacg
     5881 caatgcggca gaagatgccg ccatccgccg cctgtttatg gatcacttga acgtggacga
     5941 agaaaccgcc gacgtactgg ttcaggaagg ttttgcaacc ttggaagaag tcgcctatgt
     6001 tcctgccgcc gaactgcttg ccattgaagg atttgacgaa gaaatcgtcg atatgctccg
     6061 caaccgcgcc cgcgatgcca tcctgaccat ggcgattgcc gccgaagaaa aactgggcga
     6121 agtgtccgac gatatgcgca acctcgaagg catagatgct gatatgctcc gcagccttgc
     6181 cgaagcaggc attactaccc gcgacgactt ggcagagctt gccgtggacg aactgattga
     6241 aatcaccggt gtaaacgaag aaaccgcaaa agccgtcatc ctgaccgctc gcgaacactg
     6301 gtttaccgaa gacaaataaa agggggtaca gatgagtaac acaaccgtag aacaatttgc
     6361 cgccgagctg aaacgccccg tcgaagacct gttgaaacag ttgaaagaag ccggcgtcag
     6421 caaaaacagc ggcagcgatt ccctgacgct ggacgacaaa cagcttctga acgcctacct
     6481 gaccaagaaa aacggcagca acggcggcac catcagcatc cgccgcacca aaaccgaagt
     6541 cagcaccgtt gacggcgtaa aagtcgaaac acgcaaacgc ggacgcactg tcaacattcc
     6601 ttctgccgaa gagttggcag cacaggtaaa agccgcccaa acccaagccg cacctgtccg
     6661 gccggagcag acggcagaag acgcggcaaa agcccgagcc gaagctgccg cacgtgcaga
     6721 agcccgtgcc aaggcagaag cggaagcggc aaaactgaaa gcggcaaaag caggcaacaa
     6781 agccaaacct gccgcgcaga aacccaccga agcaaaagcc gaaaccgcac ccgttgcggc
     6841 ggaaaccaaa cccgccgaag aaagcaaagc ggaaaaagcc caagccgaca aaataccgtc
     6901 taaaaaaccc gccgagccca aagaaaaagc cgccaagccg aaacacgagc gaaacggcaa
     6961 aggcaaagat gccaaaaaac cggcgaaacc tgccgcaccc gccgccccgc aacccgtggt
     7021 cagcgcggaa gaacaggcgc aacgcgacga agaggcgcgc cgtgccgccg cacttcgcgc
     7081 ccaccaggaa gccctgttga aagagaaaca ggaacgccag gcacgccgcg aagccatgaa
     7141 acaacaggca gaacaacagg caaaagccgc acaggaagcc aaaaccggca gacagcgtcc
     7201 cgccaaacct gccgagaaac cgcaggcagc cgcgccggcc gtcgaaaaca aacctgtcaa
     7261 tccggcaaaa gcgaaaaaag aagaccgccg caaccgcgat gacgaaggtc aaggccgcaa
     7321 cgccaaaggc aaaggcggaa aaggcggacg cgaccgcaac aatgcacgca atggcgacga
     7381 cgagcgcgta cgcggcggca aaaaaggcaa aaaactcaaa ctcgagccga accaacacgc
     7441 cttccaagca ccgaccgaac ctgtcgtaca cgaagttttg gtgcctgaaa ccattaccgt
     7501 tgccgatttg gcgcacaaaa tggcagtcaa aggcgtggaa gtggtcaaag ccctgatgaa
     7561 gatgggcatg atggttacca tcaaccaatc catcgaccaa gacaccgccc tgattgtcgt
     7621 cgaagaactc ggacacatcg gcaaacccgc cgcagcggac gaccccgaag ccttcttgga
     7681 cgagggtgcg gaagcagtgg aagccgaagc attgccgcgt ccgcccgttg ttaccgtgat
     7741 gggtcacgtc gaccacggca aaacctcgct gctggactac atccgccgta ccaaagtggt
     7801 acagggcgaa gcaggcggca ttacccaaca catcggtgca taccacgttg aaaccccacg
     7861 cggcgtgatt accttcttgg atactccggg tcacgaagcg tttactgcca tgcgtgcgcg
     7921 cggtgcgaaa gcaaccgata tcgtgattct tgtggtcgcc gccgacgacg gcgtgatgcc
     7981 gcaaaccatc gaagcgattg cccacgccaa agcggcaggc gtaccgatgg tggttgccgt
     8041 caacaaaatc gataaagaag ccgccaaccc agagcgtatc cgccaagagc tgaccgcaca
     8101 cgaagtcgtg cctgacgaat ggggcggcga tgtgcaattt atcgacgttt ctgcgaaaaa
     8161 aggaacaaac atcgacgcgc tgctcgaagc agtcttgctc gaagccgaag tattggaact
     8221 gaccgcacct gtcgatgcgc ccgccaaagg catcatcgtc gaggcgcgct tggacaaagg
     8281 ccgcggcgcg gttgccacat tgctggttca aagcggtacg ctgaaaaaag gcgacatgct
     8341 gctggccggt acggcattcg gcaaaatccg cgcgatggtc gatgaaaacg gcaaatccat
     8401 taccgaagcc ggcccgtcca tccccgtcga aatcctcggc ttgtccgacg taccgaatgc
     8461 gggtgaagac gcgatggtat tggcggacga gaaaaaagcg cgcgaaatcg ccctcttccg
     8521 ccaaggcaaa taccgcgacg tgcgccttgc caaacagcag gcggcgaagc tggaaaatat
     8581 gttcaacaat atgggcgaaa cccaggccca atctttgtcg gtcatcatca aggcagacgt
     8641 tcagggctct tacgaggctt tggcgggcag cctgaaaaaa ctgtccaccg acgaggtgaa
     8701 agtgaacgtg ttgcacagcg gcgtaggcgg cattaccgaa tcggatgtca accttgccat
     8761 cgcttcgggc gcgttcatta tcggctttaa cgtgcgtgca gatgcctctt cgcgcaaact
     8821 tgccgaaaat gaaaacgtgg aaatccgcta ctacaacatc atctacgatg ccatcgacga
     8881 cgtgaaagcg gcgatgaacg gcatgctttc tccggaagag aaagaacagg ttaccggtac
     8941 ggtcgaaatc cgtcaggtca tctccgtttc caaagtcggc aacattgcag gctgtatggt
     9001 taccgacggc gtggtcaaac gcgattccca tgtccgcctc atccgcaaca acgtggtcat
     9061 ccacacgggc gaactggctt cgttgaaacg ctataaagac gacgtcaaag aagtccgtat
     9121 gggctttgag tgcggcctga tgattaaagg ctacaacgaa attatggaag gcgaccaact
     9181 cgaatgtttc gacatcgtcg aagtggcacg caccctgtga ttttgatgcg gaaaaatgcc
     9241 gtctgaagcc ttcagacggc atacgaaacg ggttctgtat catacagaac ccgttttttg
     9301 tcgcaaatcg gcttcagaca gccctcttgc cttatcccga tttgaatctg acttgccata
     9361 caaacaggct tcagacggca ttatttgccc gctaaacgta tcccaagctt ctccgcatat
     9421 tccctgcgtt cggcgtggct ggtttcgggg cggtgcgtat tgagcgacga ccatttccaa
     9481 tgactgcgag ctttgttgag ttcgggcggg agtctggcgg cgtcccacgg ggctttgcgg
     9541 ctgtgcagct caatatccga ctgtgccgcg tgtccgcgcg tttgcaggac gtggagcaga
     9601 tcgagggcgc gggcggcgag cagggtcagg gtttcggggt cggtgtgcaa ggtttggcgg
     9661 ccggcgagtt tgtcggaaat ggtgcgggta ttgggcagga tgccgcccaa aaagccgccg
     9721 attgccgtac ccaatccgag cgagccgccg agtgtggcga tgtccagccc caagccgatg
     9781 agtgcgccgg ttgccgcgcc cgtgccggtg cggatgccgt attgtttgag caattcgctg
     9841 tcgaacgggt cttggcggaa ggcttgcggc atccagtcgc cgccgtcgat ttcgctgtgg
     9901 tagaaacggt agagggcaaa cagccgctgc tgcatctgcc gttcgagttg gcgtatttcc
     9961 gcctgcatgg tttgcagcac ggtggcggta tcctcgtttt cgtccacttc ctgcctgaag
    10021 gcggcggcat caattaaaaa gtcggcgatt tcgcggcgcg cttcgccgtc cagccgctgc
    10081 cattcgcgcc ggcgcatggc tgtcaggcgg tcaagtgtgc tgcgttcggg caacatggtg
    10141 gcgaggtttt cccacaggcg tagttcgcct tcaaaatcaa aggcgacggt gtcgaaccct
    10201 gcgaaaacgt gcaggtttct cctcgccagc atggttgtcc acgattcggg aggctgtccg
    10261 ccggtaaagt tgaacacggg cataaccggt ttggcacacc atgaaaggat ggtcaactcg
    10321 tccctgtatt tgtcgaggac gggttcgcgc gcgtcgatga cgtacattgc catatcgctt
    10381 tgcaagactt gccgtaagac tttggcttcc tgattgaaat catggtgcgc gccgtggctg
    10441 ccgagaaact gttgcagccg ttcgatgccg tctgaacgat tgtccgtatg gttttccagc
    10501 cattccagca cgccgcccgc gtcttcgagt ccgggcgtgt cgtacaggaa aaccagcgtg
    10561 tctgcgccgt cgctgatggc ggcttcttcg acatgacgcg tggtcgatgg ggcgtttttg
    10621 acttcgccga aaccgctgtc gcgcaaaagg gtacgcagga gcgaggtttt gccggtgttg
    10681 gtgtgtccga cgacggcgag ggaaaggggt tgtttgttca tgatgttttt gaagaatgga
    10741 ttttcagacg gtcttttttc agaatggcgg cttaacagaa catttcaagt gagtttattg
    10801 gtctttcaaa cggccttcct gcgccgccct gtcaggctca agccacgccg cgccgcattc
    10861 ggtcagcgcg ttacgccaat gttccagctt ttccgaaagg tcgtctgaaa gcccctgttc
    10921 cgccaaaagc tgcaccaccg cgccgccctg cgccgcttcc gaaagtcgga cgatctgccg
    10981 caacacgccg cggtcgggca cagtttgggc gcgcacgccg ataagcagtt gcgccggttt
    11041 ctgcttcagc tctgtctcca gcgcggcaac ctgttcccga ttggcggcaa cgcccttatc
    11101 cagccattcc tgcgccagcc tgccctcgaa ccattcgccg tcctgccatt cggtctccag
    11161 catgaccgcc catttcggcg catcgttcaa gacgattttc ggcgaaacgg cggacacggt
    11221 ttcccgacgc gtatccgcat cggtgatttt gttctgccag cggcggatga ccgcctgata
    11281 atagggtttt tccaaatcca atccgttttc gcttgttttc aaaaggattt tgcataccgc
    11341 ccaagccaag aggcgcggca ggatgccgta gcaggcgata ctgccgacca gcagccccga
    11401 ccaagcccgc gcatcggcaa tattgccgtt cagacgacct tcgatgaccg cccgcgcatc
    11461 aggcacggga aaacccagtt tcgcaggcag ccatgccaac atttccacca gccgtaccga
    11521 agacgaatcg cccaacagcg tgctttccca gttgaacgta tattgccgca ccaaaagcag
    11581 caacaatacc gacaccagca ttccgagcag cgtgcagagc cacaggctgt gcgacgttgc
    11641 acctattttc caacgtaccg aaggttgccg ccactcgtcc gcatacagcc gcaacaccgc
    11701 ctgattgaca gggtctttgc cccgaaacca cgtcgccgga ctgctgaaaa aacgccccac
    11761 tttcacgcgc aggaacaaca ttgccaacca tactgccagc atcagcgtat tcatgcccaa
    11821 cacgcccgcc aaaaccaaaa agaaattcag accctgattg tccattagaa gataagtacc
    11881 tgaaaaaccg gtaaaaaacg caaacgtcgc cgccgccacc cacaaccaga acgaccccgc
    11941 acgcacacgt tccaatgtgt cccgcagcat acggttcctg tcgatcatct ccgcccgacg
    12001 gatgattttt tcctccgtac tgccgtccac gcggcgcaaa gcctccgtcg cctgcacggg
    12061 atcgccgctg aaaataaaac cgccttcgtc caaaatacgg atcagctcaa ccagttttcg
    12121 ggatggattc aacataaaat gccgtctgaa aataaaaaac agattttaac acacgcattt
    12181 tcaagaatat tcacagtgta ggcaaagagt aaatctcaca cagaggcaaa agtatcggcg
    12241 taaactgact gcctctactt tcccgaaaga ttgtgcgatg tatacaggcg aacgcttcaa
    12301 tacttacagc catttgagcg gtttgattct ggcggcggca ggtttggcgc tgatgttgct
    12361 gaaaaccata ggacacgggg acggctaccg tatcttcagc gtatcggttt acggcatcag
    12421 ccttcttctg ctctatttga gttcctcgct gtaccacgga attgcagccg gaaaactgaa
    12481 aagcattttg aaaaaaaccg accactgcat gatttatgtc ctgattgccg gaagctacac
    12541 accgtttgca ctggtttctt tgagaaacgg gccgggctgg acggtatttt cactgtcctg
    12601 gctgctggcg gctgcaggaa tcgcacaaga actcaccatc ggacggaaaa gcgaaaaacg
    12661 tctgctgtct attgtgattt atgtcgtcat gggttggatg gtcttggcgg taatgaaatc
    12721 cctgacagcc tcactcccgt cggcaggact ggcttggctg gcggcaggcg gtatgctgta
    12781 cagcgtcggc atttactggt ttgtaaacga tgaaaaaatc cgacacgggc acggaatctg
    12841 gcatctgttc gtattgggcg gcagcatcac ccaatttgtc agcgtgtacg gttacgtaat
    12901 ctgaatgccg tctgaaaagc aaaacctccc gatcctgaag attgggaggt tttctgtttg
    12961 ccggacatca gcccttgtcg tggaactcgt gaaattcata ctgataggac aaatcccgac
    13021 ccgctttttt ctgtgccaaa taatcatcat aaatggcacg gatttcctta cgcaacaaaa
    13081 acagggctat caagttcggg ataaccataa agccgttgaa catatcggac aggctccaaa
    13141 ccaaatcgac tttgccgagc gtacccaaaa cgatggcaag cagaaccaat gcgcgataga
    13201 tgcccaagtg tcttcccctg aaaagaaaac ggatgttgga ctcaccgaaa taataccagc
    13261 cgataatggt cgtcaaggcg aaaaaggtca gacacacggc aagcaattgc gaaccgaagc
    13321 ccggaaatgc cttgttaaag gcaaattgag taaccgccgc gccctgttcg cccgaaaggt
    13381 tggcatcagt cagcaggata atcaatgccg tagccgtaca taccaaaatc gtatcgataa
    13441 acacaccgac aaatgccgcc ataccttgct gcacaggatg cttcacatca gcggttgcat
    13501 gtgcatgcgg ggtagaaccc atacctgctt cgttggaaaa cagaccgcgc gccacgccga
    13561 aacgtatcgc ttcacgcata ccgatacccg cagcaccgcc caaaacggct tcaggattga
    13621 aggcggcggt aaagatgtgg ttgaacatcg gcacaatata gtcggaaaat tcaaacagga
    13681 taacgacggc gcacaaaata taaacaaccg ccataaacgg cacgacaaac tgggcaatat
    13741 tggcaatacg gttcacgccg ccaatcacaa ccatgcccgc aaggacggca agcacaatac
    13801 cgactgccaa agaaggcaca tcaaatgcaa tggtaacggc agaagcaatg gagtttgcct
    13861 gtgtcgcatt accgataaag cccaatgcga taatcaacgc aatggagaaa aagcccgaca
    13921 ggaaacgcgc cgcgcccctg ccgattttcg gagtcagacc gtgcgtaata taaaacgccg
    13981 gcccaccgat gtatttgccg tggctgacga cgcggtattt ctgcgccagc agtgcctccg
    14041 caaaaatcgt ggacatcccc aaaacggcag aaacccacat ccaaaaaatc gcgcccggcc
    14101 cgcctgcggt gatggcggtc gccacgccgg cgacgttgcc cgtaccgatt tgcgcggata
    14161 tggcaaccgc caacgcctga aactgcgata aagacttatc gtctttatcg cctttggcaa
    14221 acaagccgcc gaatacagat ttgaatcccg cacccagctt ggtaatctgc ggcgcaccaa
    14281 gatacagcgt aaaaaacagg ccgataccca aaagcgcgta aatcagcagg tagtcccaaa
    14341 ggaaccgatt gactgtaccc accagaacag acaatatatt ttccataaaa taaaccttat
    14401 cttacaatta aaatgactgc cttccaaaag acattccaat aaggaaacac ggcgagcaga
    14461 ccgtatttgc cgcaacagat gccttaaatt gtcaacaatc ggggagaagc tgcgccatca
    14521 taccgtaaaa tatcgttaat taaaacattt ctttattttt aagcggaaag cggaggaaat
    14581 cgctttcaga cagcatagac aacggcacgg cataaaacag gatattttgg gtacttgcaa
    14641 cttatgttaa aatgccgacc gtaaaaaatc tgacaaaaac agattaatta tttgaaataa
    14701 gaaaggaaat ttatggcagg ccatagcaag tgggcaaata tccagcataa aaaagcccgt
    14761 caggatgcca aacgcggcaa aatctttacc cgtttaatca aagaaatcac cgttgcggcg
    14821 cgtatgggcg gcggcgaccc cggttcaaat ccgcgcctgc gcctggcttt ggaaaaagca
    14881 gccgaaaaca atatgcccaa agacaatgtg caacgcgcca tcgacaaagg cacgggcaac
    14941 ttggaaggcg tggaatacat cgagttgcgc tacgaaggct acggcatcgg cggcgcggct
    15001 ttgatggtgg actgcctgac cgacaacaaa acccgcaccg ttgcggacgt acgccacgcg
    15061 tttaccaaaa acggcggcaa cttgggtacc gacggctgcg tggcgttcaa cttcgtgcat
    15121 cagggctatt tggtattcga acccggcgtt gacgaagacg cgctgatgga agcggctttg
    15181 gaagccggtg cggaagacgt ggttaccaac gacgacggtt ccatcgaagt cattaccgcg
    15241 ccaaacgatt gggcgggcgt aaaatccgct ttggaggcgg caggttacaa atccgttgac
    15301 ggcgacgtta cgatgcgcgc ccaaaacgaa accgaactct ccggcgacga tgccgtcaaa
    15361 atgcaaaaac tgattgacgc gctggaagac ttggacgacg tgcaagacgt ttacacttcc
    15421 gccgtattga atctggactg atacgcagca cagcagacat acaataaaat gccgtctgaa
    15481 cctttcagac ggcattgatt tatttagccc ttgcccacgc ccaccaaacc gtcagggcaa
    15541 ccgccagaaa atacgccaca ctccaaacag gcaaagcaac ccccagcaga taatccggtt
    15601 cggcacaatt tccgaacccg cgcacgacag gctcgaacca atcaaacaaa ggccagccct
    15661 tcaatcgaaa cgtccacggc gcaccgcacg aaggagccgt acccggcggc agcgactgca
    15721 gccacaactg atatgccgca acagaaatac ccgtaacggc cggaatactg ataaagacag
    15781 caccgaacaa accgcccgcc cttcttcttg gtctgcacat caggacaatt gccgtacaca
    15841 atgcggttgc caaaacgcat aaccgctgac tgatacacaa aacgcaaggc tccataccca
    15901 aaacatactg tgccgccaaa gaaccggcaa atgcacagac cgaaacggca aacagcagcc
    15961 aaacggcttt tctaaataac ggggtcattt tctcaacaca ccaatcaaaa taccgatatg
    16021 ccgattttgc tggatatatc ccgagatggc aagggacgaa tcgacaccgg ctcaggtgcg
    16081 gacgatttcg gttttccccg atatgcataa aacctgacaa gggacaaaac ggcgatttgc
    16141 ccgcacaatg cccgcaaaaa aatacatccg gaggatttga atttcggcaa atttagcgaa
    16201 tcaaaagttt atttcaatga aattatatga tttttttgaa taagcggatt gacaggtttt
    16261 tgaaggaatt tgttaccgga tagccatcgg gcaagttttt tgcaaaattt gaatcggtcg
    16321 ggtaaatttt caaaaaataa ttgacagcgg ataagaaacg gcggataatt cccgccgtcg
    16381 agttgcttga tgcagcttga tttttctcct ctatttctcc tttgtagact tggcacacat
    16441 tcaactggat gtgtgcattt ttttatctcc gctttttttg aaatttaatt acttttattg
    16501 tttgaaattc cattctttaa gaaatttaac aagaaaaacg cttgcttttt gaccggaaaa
    16561 gctgcataat tcgttccgtt agctggttcg agtagtcagt taatagtttc tcctctattt
    16621 ctcctttgta gacttggcac acattctatt gaatgtgtgc atttttttat ctgaagcaac
    16681 aagcctctgt gcgtgatgtt gttatgtttc atttaggtgt caaaccgcat acccggtctg
    16741 aaatattcaa tccaaatcca aaactggatt ttctttgacc tcctccatca caacataact
    16801 cctactctcc gaagcggcag gcagttgcaa taggatattg cctagcatat cccgataggc
    16861 agacatatcg ggcaaacgta ctttaatcag atagtcgtat tcgcccgaca ccaagtggca
    16921 ttccataatt tgcggaattt tcagcacttc ttttttgaaa tcttcgaaaa tattgcccga
    16981 tttggattgc agcttcagct cgacaaaaac caatagtggt ttgcccaaca gatggggatt
    17041 gagatgggcg tgatagccgg aaatataatg ttcccgctcc aaacggcgca ccctctctgt
    17101 aacgggcgtg gtggacaagc ctaccttctc ggcaagctcc atcatcggga tgcgggcatt
    17161 ctgttggagg atcttaagaa tgcggaaatc gattttatct agttctttca tttggattgc
    17221 cttgtattta ttattgattt taacaaatag a
//
LOCUS       DAXHSF010000024        17007 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.024, whole genome shotgun sequence.
ACCESSION   DAXHSF010000024 DAXHSF010000000
VERSION     DAXHSF010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17007
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.024"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            208..1272
                     /locus_tag="ACFM8S_000651"
     CDS             208..1272
                     /locus_tag="ACFM8S_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HFC6313758.1"
                     /translation="MKAARFYDKGDIRIEDIPEPTVAPGTVGINVAWCGICGTDLHEF
                     MEGPIFIPPCGHPHPISGESAPVTMGHEFSGVVYAVGEGVDDIKVGQHVVVEPYIIRD
                     DVPTGEGSNYHLSKDMNFIGLGGCGGGLSEKIAVKRRWVHPISDKIPLDQAALIEPLS
                     VGHHAYVRSGAKAGDVALVGGAGPIGLLLAAVLKAKGIKVIITELSKARKDKARESGV
                     ADYILDPSEVDVVEEVKKLTNGEGVDVAFECTSVNKVLDTLVAACKPAANLVIVSIWS
                     HPATINVHSVVMKELDVRGTIAYCNDHAETIKLVEEGKINLEPFITQRIKLDELVSEG
                     FERLIHNNESAVKIIVSPNL"
     gene            1413..1736
                     /locus_tag="ACFM8S_000652"
     CDS             1413..1736
                     /locus_tag="ACFM8S_000652"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HFC6313759.1"
                     /translation="MGDSVLSAIQQTITQRKSANPSESYVAQLLHKGEDKILKKVIEE
                     AGEVLMASKDKNPSHLVYEVADLWFHTMILLTHHDLKAEDVLDELARRQGLSGLAEKA
                     ARTES"
     gene            1808..2131
                     /locus_tag="ACFM8S_000653"
     CDS             1808..2131
                     /locus_tag="ACFM8S_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HFC6313760.1"
                     /translation="MDNCIFCKIAAKDIPAQTVYEDGEMVCFKDINPAAPVHLLLIPK
                     VHFDSLAHAAPEHQPLLGKMMLKVPEIAKAAGLADGFKTLINTGKGGGQEVFHLHIHI
                     MGTPV"
     gene            2178..2381
                     /gene="tatA"
                     /locus_tag="ACFM8S_000654"
     CDS             2178..2381
                     /gene="tatA"
                     /locus_tag="ACFM8S_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFC6313761.1"
                     /translation="MGSFSLTHWIIVLIIVVLIFGTKKLRNVGKDLGGAVHDFKQGLN
                     EGTDGKEAQKDDVIEHKKDEDKA"
     gene            2385..3071
                     /gene="tatB"
                     /locus_tag="ACFM8S_000655"
     CDS             2385..3071
                     /gene="tatB"
                     /locus_tag="ACFM8S_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFC6313762.1"
                     /translation="MFDFGLGELVFVGIIALIVLGPERLPEAARTAGRLIGRLQRFVG
                     SVKQEFDTQIELEELRKAKQEFEAAAAQVRDSLKETGTDMEGNLHDISDGLKPWEKLP
                     EQRTPADFGVDENGNPLPDAANTLSDGISDVMPSERSYASAETLGDSGQTGSTAEPAE
                     TDQDRAWREYLTASAAAPVVQTVEVSYIDTAVETPVPHTTSLRKQAISRKRDLRPKSR
                     AKPKLRVRKS"
     gene            3085..3855
                     /gene="tatC"
                     /locus_tag="ACFM8S_000656"
     CDS             3085..3855
                     /gene="tatC"
                     /locus_tag="ACFM8S_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HFC6313763.1"
                     /translation="MSETQNEQPVQPLVEHLIELRRRLMWTVVGILVCFFGMMPFAQQ
                     LYTFIADPLMANLPKDTSMIATDVIAPFFVPVKVTLMAAFLISLPHTLYQIWAFVAPA
                     LYQNEKRLITPLVLSSVSLFFIGMAFAYFLVFPVIFKFLASVTPVGVNMATDIDKYLS
                     FILGMFVAFGTTFEVPIVVILLTKISVVTTGQLKRARPYVIVGAFVIAAIITPPDVIS
                     QTLLAIPLILLYEAGIWFGRFFTPRSEQDGDIRPPATT"
     gene            3908..4513
                     /locus_tag="ACFM8S_000657"
     CDS             3908..4513
                     /locus_tag="ACFM8S_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HFC6313764.1"
                     /translation="MNTLYLGSNSPRRMEILTQLGYRVIQLPAGIDESVKAGETPFAY
                     VQRMAEEKNRTALTLFCETNGTMPDFPLITADTCVVSDGIILGKPRSQAEAIEFLNRL
                     SGKQHTVLTAVCIHYRGNAENRVQTNRVVFKPLSSEEISAYVQSGEPMDKAGAYAVQG
                     IGGIFIQSIEGSFSGIMGLPVYETVSMLQDLGYRTPPFIRA"
     gene            4529..4882
                     /locus_tag="ACFM8S_000658"
     CDS             4529..4882
                     /locus_tag="ACFM8S_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HFC6313765.1"
                     /translation="MNRQTAYLLTSFSLIALIILSLAWELWIAPLRPGGSWLALKTLP
                     LCLPLSGILKKKIYTYQYSSMLILIYFAEAVMRLFDAYPAEQICAALSAVFSIIFFIS
                     CLSFVKQYKETNNAR"
     gene            4872..6686
                     /locus_tag="ACFM8S_000659"
     CDS             4872..6686
                     /locus_tag="ACFM8S_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy polymerase domain-containing protein"
                     /protein_id="HFC6313766.1"
                     /translation="MPAETPVSGARPAAKLPIYILPCFLWIGIVPFTFALRQQPSPDF
                     YHDAAAAAGLIVLLFLTAGKKLFDVKIPPISFLLFAMAAFWYLQARLMNLIYPGMNDI
                     VSWIFILLAVSAWACRSLVAHYGQERIVTLFAWSLLIGSLLQSCIVVIQFAGWEDTPM
                     LQNIIVYSGQGVIGHIGQRNNLGHYLMWGILASAYLNGQRKIPAALGVICLIMQTAVL
                     GLVNSRTILTYIAAIALILPFWYFRSDKSNRRTMLGIAAAVFLTALFQFSMNTILETF
                     TGIRYETAVERVANGGFTDLPRQIEWNKALAAFQSAPIFGHGWNSFAQQTFLINAEQH
                     NIHDNLLSNLFTHSHNIVLQLLAEMGISGTLLVAATLLTGIAGLLKRPLTPASLFLIC
                     TLAVSMCHSMLEYPLWYVYFLIPFGLMLFLSPAEASDGIAFKKAANLGILTASAAIFA
                     GLLHLNWTYTRLVNAFSPATDDSAKTLNRKINELRYISANSPMLSFYADFSLVNFALP
                     EYPETQTWAEEATLKSLKYRPHSATYRIALYLMRQGKVAEAKQWMRATQSYYPYLMPR
                     YADEIRKLPVWAPLLPELLKDCKAFAAAPGHPEAKPCK"
     gene            6876..7553
                     /gene="misR"
                     /locus_tag="ACFM8S_000660"
     CDS             6876..7553
                     /gene="misR"
                     /locus_tag="ACFM8S_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator MisR"
                     /protein_id="HFC6313767.1"
                     /translation="MSRVLLVDDDALLTELLTEYLSAEGLNVRSVPDGEAGVQEILSG
                     QYDVVVLDSMMPKMNGLDVLKNVRARSTVPIIMLTAKGDDIDRIIGLEMGADDYVPKP
                     CTPRELLARINAILRRAQHSGEQNNAPNSISVSDVVLYPAKRQASVKDMPLELTSTEF
                     NLLEVLMRHAGQVVSKETLSVEALDRKLAKFDRSIDVHISSIRHKLGDASLIQTVRGL
                     GYLFVKN"
     gene            7567..8973
                     /gene="misS"
                     /locus_tag="ACFM8S_000661"
     CDS             7567..8973
                     /gene="misS"
                     /locus_tag="ACFM8S_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     MisS"
                     /protein_id="HFC6313768.1"
                     /translation="MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTI
                     ETTLMGSIISAFRARGDAGAREILTEWKDSPVSSGVYVIQGDEKKDILNRYIDSYTIE
                     RARLFAAGHPHSNLVHIEYDRFGEEYLFFTKDWDKLQARRLPSPLLIPGLPLAPIWHE
                     LIILSFIIIVGLLMAYILAGNIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSH
                     LAIQFDKMVEKLEKLVAKERHLLHHVSHEMRSPLARMQAIVGLIQAQPQKQEQYLKRL
                     EGELTRMDTLAGELLTLSRLETSNMALEKESLKLLPFLGNLVEDNQSIAQKNGQTVTL
                     SADGKIPENTTILANESYLYRAFDNVIRNAVNYSPEGSTILINIGQDHKHWIIDVTDN
                     GPGVDEMQLPHIFTAFYRADSSANKPGTGLGLALTQHIIEQHCGKIIAENIKPNGLRM
                     RFILPKKKTGSKTEKSAN"
     gene            9091..11517
                     /locus_tag="ACFM8S_000662"
     CDS             9091..11517
                     /locus_tag="ACFM8S_000662"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC6313769.1"
                     /translation="MSAQTDPGYFFMPNHIILIGASERPYSLGERVLSNLLSTPFQGK
                     ITPVNPRHHTIAGLPAYTSLNKIPGNADLIIAVTLPDSYDTLFKTCRKKQLRHIILIQ
                     DWDSLSAAELHTAETAIRKHHGNGLNITACTTAGIQLPSLGLNISTQDGYAAGHTAIL
                     TGNAAVSRQIDNILNKLRQGTSRHISLHPGISPITSADWLNRFGHSLHTKTAVLHHNP
                     EEDQRKLFSAIRQFTRHTPLILHITCRTTETDRAVLHCLARHCNFLISFNADDLEAAL
                     RAQLSDLPPLSRLDILSDTPAEWLNAHAPKTLALHFPNLPHHIRNGHLTGTPTPSICH
                     DIASRQLARPDTQAVLTILSPSGHEDYKKTARALIRLSEQTAKPLLVSSPFSDGITHF
                     DTPTQAIRTLSYRNTAAALKQAQLDIAPPQPCRLKTPQPQNIKKALAAANPSLLAEAL
                     HLPPYRHTTHNAVQFQFGSHPIYGNILTVRYAGQTTAALPPFTTQDSLHIAQFAELED
                     PQTLNQFLHILTTLSDHNLHIGKITLNLNGGQYSSDFILKTPETHDTPKRKNTGKAAQ
                     TLEHAAAKMQSAAAYLKHKNPTAAEFLRHTSEAAAELLGSKTETGAAVPNVLAPYPAA
                     HPKTLSLKNNTTVTITPLLPEDAEAKQQFVRSLGPEARYTRFMTHTNELPAATLARLC
                     NPDYHCEAAWTAKDADSNIVAVVRHSRLNRNECEFGITLAEHMRGSGLAQKMMELIIQ
                     TAAQQGYRTMSADILKTNTPMIKLAEKSGFTLKESDTEKNLYRAYLNLAADKTTEKTN
                     KNLRTDHKIT"
     gene            11562..11807
                     /gene="rpsP"
                     /locus_tag="ACFM8S_000663"
     CDS             11562..11807
                     /gene="rpsP"
                     /locus_tag="ACFM8S_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFC6313770.1"
                     /translation="MVVIRLARGGSKHRPFYNVIVTDSRSRRDGRFIERVGFYNPVAN
                     EKQERVRLNADRLNHWIAQGAQVSDSVAKLIKEQKAA"
     gene            11823..12332
                     /gene="rimM"
                     /locus_tag="ACFM8S_000664"
     CDS             11823..12332
                     /gene="rimM"
                     /locus_tag="ACFM8S_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748672.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFC6313771.1"
                     /translation="MTDTQNRVAMGYIKGVFGIKGWLKIAANTEYSDSLLDYPEWHLV
                     KDGKTISVTLEAGKVVNGELQVKFEGINDRDLAFSLRGYTIEIPREAFAPTEEDEYYW
                     TDLVGMTVVNKDHTVLGKVSNLMETGANDVLMIDGEHGQILIPFVSQYIETVDTGSKI
                     ITADWGLDY"
     gene            12332..13081
                     /gene="trmD"
                     /locus_tag="ACFM8S_000665"
     CDS             12332..13081
                     /gene="trmD"
                     /locus_tag="ACFM8S_000665"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFC6313772.1"
                     /translation="MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADN
                     RLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKAQSSQAAKVIYLSPQGKPLTHPKAAEL
                     AELPHLILLCGRYEGIDERLLQTSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGVL
                     GDMQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLE
                     RRPDLLEKRVLIPKESRLLETIRQEQREIQS"
     gene            13097..13462
                     /gene="rplS"
                     /locus_tag="ACFM8S_000666"
     CDS             13097..13462
                     /gene="rplS"
                     /locus_tag="ACFM8S_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFC6313773.1"
                     /translation="MNLIQQLEQEEIARLNKEIPEFAPGDTVVVSVRVVEGTRSRLQA
                     YEGVVIARRNRGLNSNFIVRKISSGEGVERTFQLYSPTVEKIEVKRRGDVRRAKLYYL
                     RGLTGKAARIKEKLPARKG"
     gene            13482..13787
                     /locus_tag="ACFM8S_000667"
     CDS             13482..13787
                     /locus_tag="ACFM8S_000667"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313774.1"
                     /translation="MPSEPSDGISYCRNIRNGTAPRSTPLPAHPESPSEPNKIYRPNV
                     IQYHISHIIITALPLSLSKPQIIMTPSLLLSGLTFRLVLALIAVSLLWGVYFWAVSA"
     gene            13784..14509
                     /locus_tag="ACFM8S_000668"
     CDS             13784..14509
                     /locus_tag="ACFM8S_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFC6313775.1"
                     /translation="MSIIVENLTVSYRRRPAVHHVDITFEEHSMWAVFGPNGAGKSTF
                     LKSLMGLQPIDTGSIRLDGLSRQDIAYLPQQSDIDRSQPMTVFDLAAMGLWYEIGFFK
                     GINTAQKQRVHESLERVGMRQFAGRQIAHLSNGQFQRVLFARMLVQNAKFLLLDEPFN
                     AVDARTTYELLDVLQKCHCGGHAIIAVLHDYEQVRAYFPSTLLLAREKIAAGATETIL
                     TEPYLAQANAKMQQQESPDWCAS"
     gene            14544..15419
                     /locus_tag="ACFM8S_000669"
     CDS             14544..15419
                     /locus_tag="ACFM8S_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFC6313776.1"
                     /translation="MNLYDLLLAPFAEFDFMRYALASVFCLSLSAAPVGVFLVMRRMS
                     LIGDALSHAVLPGAAVGYMFAGLSLPAMGLGGVAAGMLMALLAGLVSRFTTLKEDANF
                     AAFYLSSLAIGVVLVSKNGSSVDLLHLLFGSVLAVDIPALQLIAAVSSLTLITLAVIY
                     RPLVLESIDPLFLKSVGGKGGLWHVLFLVLVVMNLVSGFQALGTLMSVGLMMLPAITA
                     RLWAKHMGALILLSVLTALLCGLSGLLISYHIEIPSGPAIILCCSVLYLFSVILGKEG
                     GILTKWLKNHRHHTT"
     gene            15496..16410
                     /locus_tag="ACFM8S_000670"
     CDS             15496..16410
                     /locus_tag="ACFM8S_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFC6313777.1"
                     /translation="MKHLKLTLIAALLTTAATAAPLPVVTSFSILGDVAKQIGGERVS
                     IQSLVGANQDTHAYHMTSSDIKKIRSAKLVLLNGLGLEAADVQRAVKQSKVSYAEATK
                     GIQPLKAEEEGGHHHDHDHDHEGHHHDHGEFDPHVWNDPVLMSAYAQNVAEALIKADP
                     EGKVYYQQRLGNYQMQLKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGKRYHIEFIA
                     PQGVSSEAEPSAKQVAAIIRQIKREGIKAVFTENIKDTRMVDRIAKETGVNVSGKLYS
                     DALGNAPADTYIGMYRHNIKALINAMKQ"
     gene            complement(16690..>17007)
                     /locus_tag="ACFM8S_000671"
     CDS             complement(16690..>17007)
                     /locus_tag="ACFM8S_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HFC6313778.1"
                     /translation="DTMHFKGFKAADVHFIRSGSDLVLSASEQDNVRISGFFYGENHR
                     VDTFVFDDAAISNPDFAKYINAGNNLVQSMSVFGSNTAATGGNVDANIQSVQQPLLVT
                     PSA"
BASE COUNT         4462 a         5389 c         3715 g         3441 t
ORIGIN      
        1 cgtctgaagc cgattcggtt ttcagacgac atcattattt gacctgacgc aaacagaaag
       61 ccttccgcgt gcttaaacac atctcttgta gtaaaatatc taaacagatt caactggaaa
      121 cggtcaggct tcttcacgcc attcccattt caaccgccgc ctgccagatt cagacggtat
      181 tcctacttga aaacaaggag cgttacaatg aaagcagcac gtttttacga taaaggcgac
      241 atccgcatcg aagacatccc cgaaccgacc gtcgcccccg gcactgtcgg catcaatgtc
      301 gcctggtgcg gcatctgcgg tactgacctg cacgaattca tggaaggccc gattttcatt
      361 ccgccttgcg gtcatccgca cccgatttcc ggcgagtccg cacccgtaac gatgggacac
      421 gagttctccg gcgtggtcta tgccgtcggc gaaggcgtgg acgacatcaa agtcggccaa
      481 cacgtcgtgg tcgaacccta catcatccgc gatgacgtac cgaccggaga aggcagcaac
      541 taccacctct ccaaagatat gaactttatc ggcttgggcg gctgcggcgg cggtctgtcc
      601 gaaaaaatcg ccgtcaaacg tcgttgggtg catccgattt ccgacaaaat cccgttggat
      661 caagccgctt tgatcgaacc gctgtctgtc ggacaccacg cctatgtacg cagcggcgcg
      721 aaagcaggcg acgtcgcatt ggtcggcggc gcaggtccga tcggtttgct gttggctgcc
      781 gtgttgaaag ccaaaggcat caaagtcatc atcaccgagt tgagtaaagc acgcaaagac
      841 aaagcgcgcg aatccggcgt tgccgactac atcctcgacc cgtccgaagt cgatgttgtc
      901 gaagaggtga aaaaactgac caacggcgaa ggcgtggacg tggcattcga atgtaccagc
      961 gtgaacaaag tgttggatac tttggtggcc gcctgcaaac ccgctgccaa cttggtcatc
     1021 gtatccattt ggagccaccc cgccaccatc aacgtccaca gcgttgtgat gaaagagttg
     1081 gacgtgcgcg gcacgattgc ctactgtaac gaccacgccg aaaccatcaa actggtcgaa
     1141 gaaggcaaaa tcaacctcga acccttcatc acccagcgca tcaagctgga cgaactggtt
     1201 tccgaaggct tcgagcgtct gatccacaac aacgaatccg ccgttaaaat tattgtgagt
     1261 ccaaacctgt aagcaaatta catttgcaac agcacaaatg ccgtctgaaa cgctcagacg
     1321 gcattttcca acgaagcgca acaggcgtat tgcccgagag cagccgttta gcctatcatt
     1381 ccgaacaaac agaaacaaac agaaggaaaa tcatgggaga ttccgtacta tccgccatcc
     1441 aacaaaccat cacccagcga aaatctgcca atccgtccga atcttacgtc gcacagctct
     1501 tgcataaggg cgaggacaaa atcctaaaaa aagtgattga agaggcgggc gaagtgttga
     1561 tggcatccaa agacaaaaac ccgtcccacc tggtttacga agttgccgac ttatggtttc
     1621 acaccatgat tcttctgaca caccacgacc tgaaggcgga agacgtattg gacgaacttg
     1681 cgcgccgcca aggtttgtcg ggcttggccg aaaaagccgc tcgcacagaa tcttgaattt
     1741 atattaaaat ccgcactttc ccacattcaa tccgtctgac cgctgttcag acggcaacgg
     1801 agccgttatg gacaactgta ttttctgcaa aatcgccgcc aaagacattc cggcgcaaac
     1861 cgtctacgaa gacggcgaaa tggtttgttt caaagacatc aaccccgccg ctccggttca
     1921 tctgctgctg attcccaaag tccatttcga ttcgttggca cacgccgcgc ccgaacatca
     1981 gccccttttg ggaaaaatga tgctgaaagt tcccgaaatc gccaaagcgg caggactggc
     2041 agacggcttc aaaaccctga tcaataccgg aaaaggcggc ggacaagagg tcttccacct
     2101 gcatatacac atcatgggca cacccgtata aaccgttatt tcacaatcaa cccctaatac
     2161 ttacttaagg atacatcatg ggcagttttt ctctgacgca ctggattatc gtactgatta
     2221 tcgtcgtttt gatattcggc accaaaaaac tgcgcaacgt cggcaaagac ctcggcggtg
     2281 cggttcatga cttcaaacag gggctgaacg aaggtacaga cggcaaagaa gcccaaaaag
     2341 acgatgtaat cgaacacaaa aaagacgaag acaaagcgta atttatgttt gatttcggtt
     2401 tgggcgagct ggtttttgtc ggcattatcg ccctgattgt cctcggcccc gaacgcctgc
     2461 ccgaggccgc ccgcaccgcc ggacggctca tcggcaggct gcaacgcttt gtcggcagcg
     2521 tcaaacagga atttgacact caaatcgaac tggaagaact gaggaaggca aagcaggaat
     2581 ttgaagctgc cgccgctcag gttcgagaca gcctcaaaga aaccggtacg gatatggaag
     2641 gcaatctgca cgacatttcc gacggtctga agccttggga aaaactgccc gaacagcgga
     2701 cacctgccga tttcggtgtc gatgaaaacg gcaatccgct tcccgatgcg gcaaacaccc
     2761 tatcagacgg catttccgac gttatgccgt ccgaacgttc ctacgcttcc gccgaaaccc
     2821 ttggggacag cgggcaaacc ggcagtacag ccgaacccgc ggaaaccgac caagaccgcg
     2881 catggcggga atacctgact gcttctgccg ccgcacccgt cgtacagacc gtcgaagtca
     2941 gctatatcga taccgctgtt gaaacccctg ttccgcatac cacttcgctg cgtaaacagg
     3001 caataagccg caaacgcgat ttgcgtccca aatcccgcgc caaacctaaa ttgcgcgtcc
     3061 gtaaatcata aagagagcaa tccggtgtcc gaaacacaaa acgaacaacc cgtccaaccg
     3121 cttgtcgagc atctcatcga gctgcgccgc cgcctgatgt ggacggttgt cggcatctta
     3181 gtctgctttt tcggcatgat gccgtttgcc caacaactct atacttttat cgccgacccg
     3241 ctgatggcaa acctgcccaa agacaccagc atgattgcca ccgatgtcat cgcaccattt
     3301 ttcgtgccgg tcaaagttac cctgatggcg gcatttttaa tttcgctgcc gcatacgctc
     3361 taccaaatct gggcatttgt cgcgcccgca ctctaccaaa acgaaaaacg cctgattacg
     3421 ccgctcgtcc tctccagcgt cagcctgttt ttcatcggca tggcatttgc ctactttttg
     3481 gttttccccg tcattttcaa attccttgcc agcgttaccc ctgtcggtgt caatatggcg
     3541 acagacatcg acaaatacct gtcttttatc ttaggcatgt ttgttgcgtt cggcacaacg
     3601 tttgaagttc ccatcgtcgt tatcctgtta accaaaatca gcgtggtaac aaccggacag
     3661 ctcaaacgcg cccgccccta tgtgattgtc ggcgcgtttg tcattgccgc catcatcacg
     3721 ccgcccgatg tgatttcaca aaccctgctt gccattccgc tgattctctt atacgaagca
     3781 ggtatttggt tcggacgctt tttcacgcca cgttcagaac aggatggcga catacggccg
     3841 cctgcaacaa cctgacacta tgccgtccga acctccgcct cataccgcaa cagattaagg
     3901 aatacctttg aataccctct atttaggttc aaacagcccg cgccgaatgg aaatcctgac
     3961 acagttgggc taccgcgtca tccaactgcc tgccggcatc gacgaatccg ttaaagccgg
     4021 cgaaacacct ttcgcttacg ttcaaaggat ggcagaagaa aaaaaccgaa ccgccctgac
     4081 cctcttttgc gaaaccaacg gcacaatgcc cgatttcccc ctgattaccg ccgacacctg
     4141 cgtcgtttca gacggcatca tattgggcaa accccgctcc caagccgaag caatcgaatt
     4201 tttaaaccga ttgtccggca aacaacatac cgtcctgact gccgtctgca ttcattatcg
     4261 cggcaatgcg gaaaaccgcg tccaaaccaa ccgcgtcgtt ttcaagcccc tgagttcgga
     4321 agaaatttcc gcctatgtgc aaagcggcga accgatggac aaagccggcg cctacgccgt
     4381 acaaggcata ggcggcatct ttatccaatc tatcgaaggc agcttcagcg gcattatggg
     4441 gctgcccgtt tatgaaaccg tttcgatgtt gcaggatttg ggataccgta ccccaccttt
     4501 catccgagcc taacccgtaa agaccgccgt gaacagacaa accgcttacc tccttacctc
     4561 tttcagcctg atcgcactga taatcctgtc ccttgcttgg gaactgtgga ttgcaccgtt
     4621 acgcccgggc ggttcgtggc ttgcgctcaa aaccctgccc ctctgcctgc cgctttcagg
     4681 catcctgaag aaaaaaatct acacctacca atacagctcc atgttgattc tgatttattt
     4741 tgccgaagcc gtcatgcgcc tgttcgacgc ctatccggca gaacaaattt gcgccgcgct
     4801 ttccgcagta ttcagcatca tcttcttcat atcctgcctg tcgttcgtca aacaatacaa
     4861 ggaaacaaac aatgcccgct gaaacgcccg tatccggtgc gcgccccgcc gccaaactgc
     4921 cgatttacat cctgccctgc ttcctttgga taggcatcgt cccctttacc ttcgcgctca
     4981 ggcagcaacc gtcgcccgac ttttaccacg atgctgcagc cgcagccggc ctgattgtcc
     5041 tgttgttcct cacggcagga aaaaagctgt ttgatgtcaa aatcccaccc atcagcttcc
     5101 ttctgtttgc aatggcggcg ttttggtatc ttcaggcacg cctgatgaac ctgatttacc
     5161 ccggtatgaa cgacatcgtc tcttggattt tcatcttgct cgccgtcagc gcgtgggcct
     5221 gccggagctt ggtcgcacac tacggacaag aacgcatcgt taccctgttt gcctggtcac
     5281 tgcttatcgg ctccctgctt caatcctgta tcgtcgtcat ccagtttgcc ggctgggaag
     5341 acacccccat gcttcaaaac attattgttt acagcgggca aggcgtaatc ggacacatcg
     5401 ggcagcgcaa caacctcgga cactacctca tgtggggcat actcgcctcc gcctacctca
     5461 acggacaacg aaaaatcccc gccgccctcg gcgtaatctg cctgattatg cagaccgccg
     5521 ttttaggttt ggtcaattcg cgcaccatct tgacctacat agccgccatc gccctcatcc
     5581 ttcccttctg gtatttccgt tcggacaaat ccaacaggcg gacgatgctc ggcatagccg
     5641 cagccgtatt ccttaccgcg ctgttccaat tttccatgaa caccattctg gaaaccttta
     5701 ccggcatccg ctacgaaact gccgtcgaac gcgttgccaa cggcggtttc acagacttgc
     5761 cgcgccaaat cgaatggaat aaagcccttg ccgccttcca gtccgccccg atattcgggc
     5821 acggctggaa cagttttgcc caacaaacct tcctcatcaa tgccgaacag cacaacatac
     5881 acgacaacct cctcagcaac ttgttcaccc attcccacaa catcgtcctc caactccttg
     5941 cagagatggg aatcagcggc acgcttctgg ttgccgcaac cctgctgacg ggcattgctg
     6001 ggctgcttaa acgccccctg acccccgcat cgcttttcct aatctgcacg cttgccgtca
     6061 gtatgtgcca cagtatgctc gaatatcctt tgtggtatgt ctatttcctc atccctttcg
     6121 gactgatgct cttcctgtcc cccgcagagg cttcagacgg catcgccttc aaaaaagccg
     6181 ccaatctcgg catactgacc gcctccgccg ccatattcgc aggattgctg cacttgaact
     6241 ggacatacac ccggctggtt aacgcctttt cccccgccac tgacgacagt gccaaaaccc
     6301 tcaaccggaa aatcaacgag ttgcgctata tttccgcaaa cagtccgatg ctgtcctttt
     6361 atgccgactt ctccctcgta aacttcgccc taccggaata ccccgaaacc cagacttggg
     6421 cggaagaagc aaccctcaaa tcactaaaat accgccccca ctccgccacc taccgcatcg
     6481 ccctctacct gatgcggcaa ggcaaagttg cagaagcaaa acaatggatg cgggcgacac
     6541 agtcctatta cccctacctg atgccccgat acgccgacga aatccgcaaa ctgcccgtat
     6601 gggcaccgct gctacccgaa ctgctcaaag actgcaaagc cttcgccgcc gcgcccggtc
     6661 atccggaagc aaaaccctgc aaatgacccg cgccggcgga tgcggatacc gcccgaaatg
     6721 taaagctcca tgcaagacat tgcaaaaaac agcaaaccgg tagggaaata cgctatcaaa
     6781 aaacattgca gccgtgttaa gataaaccgt caaacaatct tttcacgccc cgcccgaaac
     6841 agggtcgggg cataccctta cgaaaaggaa acaccatgag ccgcgtatta ctcgtagatg
     6901 acgatgccct gctaaccgaa ctgctgaccg aatacctgag cgccgaaggt ctgaacgtcc
     6961 gcagcgttcc cgacggggaa gcaggcgtac aggaaatcct gagcgggcag tacgatgtag
     7021 tcgtattgga ttccatgatg cccaaaatga acggcttgga tgtcttgaaa aacgtacgcg
     7081 cccgaagcac cgtccccatc atcatgctga ccgccaaagg cgacgacatc gaccgaatca
     7141 tcggcttgga aatgggcgcg gacgactatg tccccaaacc ctgcacacca cgcgaactct
     7201 tggcacgcat caatgccatc ctgcgccgcg cacaacacag cggcgaacag aacaacgcac
     7261 ccaacagcat ctccgtcagc gatgtcgtcc tgtaccccgc caaacgccag gcatccgtca
     7321 aagacatgcc gctcgaactg accagcaccg aattcaacct gctcgaagtc ctgatgcgcc
     7381 atgccggaca ggtagtcagc aaagaaaccc tgtccgtcga agcactcgac cgcaagctgg
     7441 caaaattcga ccgcagtatc gacgtacaca tctccagcat ccgccacaag ttgggcgatg
     7501 cctctctgat tcaaaccgta cgcggcttgg gctacctgtt tgtcaaaaac tgaaataaac
     7561 agataaatga aactgttcca acgcatcttc gccacatttt gcgcggttat cgtctgtgca
     7621 atctttgtgg cgagtttttc tttctggctg gtgcagaaca cccttgccga aaaccagttc
     7681 aaccaacgcc gcaccatcga aaccactttg atgggcagca tcatttccgc attccgggca
     7741 cgcggggacg cgggcgcgcg cgaaatcctg acggaatgga aagacagccc cgtctcatcg
     7801 ggcgtgtacg ttatacaggg cgacgagaaa aaagatatcc tgaaccggta tatcgacagc
     7861 tataccatcg aacgcgcccg gcttttcgcc gccggacacc cgcattccaa cctcgtccat
     7921 atcgaatacg accgcttcgg cgaagaatac ctgttcttca ccaaagactg ggacaaactc
     7981 caagcccgcc gcctgcccag ccccctgttg atccccggcc tgccgctcgc cccgatttgg
     8041 cacgaactca tcatattgtc cttcatcatc atcgtcggac tgctgatggc atatatcctc
     8101 gccggcaaca ttgccaaacc catcagaatc ttaggcaacg gcatggacag ggtggcaaac
     8161 ggagaacttg aaacccgtat ctcccaacag gtcgacgacc gcgacgacga attgtcccat
     8221 cttgccatcc aattcgacaa aatggtggaa aaactcgaaa aactcgttgc caaagaacgc
     8281 cacctgctcc atcacgtctc ccatgaaatg cgttctcccc ttgcgcgcat gcaggcaatt
     8341 gtcggactga ttcaggcgca gccccaaaaa caggagcaat atctcaaacg gctggaaggc
     8401 gaactgaccc gcatggatac gctggccggg gaactgttaa ccctgtcccg tctcgaaact
     8461 tccaatatgg ctttggaaaa agaaagcctg aaactcctgc ccttcctggg caacctggta
     8521 gaagacaatc aaagcattgc ccagaaaaac ggacaaacgg ttaccctgtc tgccgacgga
     8581 aaaatccccg aaaatacaac catccttgcc aacgaaagct acctgtaccg cgccttcgac
     8641 aacgtcatcc gcaacgccgt caactacagt cccgaaggca gcaccatcct gatcaacatc
     8701 ggacaagacc acaaacactg gataatcgac gttaccgaca acggccctgg cgtggacgaa
     8761 atgcagctcc cgcacatctt caccgctttc taccgtgcag actccagtgc gaacaaaccc
     8821 ggaacaggac tggggcttgc attgacccaa catattattg aacagcactg cggaaaaatc
     8881 atcgccgaaa acatcaaacc gaacggtctg cggatgcgct tcatcctgcc caagaaaaaa
     8941 accggttcca aaacagaaaa aagtgcgaac tgaccataat gccgtctgaa ccggcttcag
     9001 acggcattgc acaaacagtt accccaaaac aaacacacag cgggtgcatc tccaacaatc
     9061 acgcacatac cgtccgcaaa ggaatatatc atgtcggcac aaaccgatcc gggctacttt
     9121 ttcatgccca accacattat tctgataggc gcgagcgaac gtccgtacag cctgggcgaa
     9181 cgcgtactca gcaacctgct gagtacgccc tttcaaggaa aaatcacccc cgtaaacccg
     9241 cgccaccaca ccatagccgg gcttcccgcc tacaccagcc tcaacaaaat ccccggcaat
     9301 gcagacctga ttattgccgt taccctaccc gacagttacg acaccctctt caaaacctgc
     9361 cgcaaaaagc agctccgaca catcatcctc atacaggact gggacagcct gtctgccgca
     9421 gaactgcaca ccgccgaaac tgccatccgc aaacaccacg gcaacggact caacatcacc
     9481 gcctgcacca ccgcaggcat ccaactgccc tcactcggac ttaacatcag tacccaagac
     9541 ggatacgccg caggccatac cgccatactg accggcaatg ccgccgtcag ccgccaaatc
     9601 gacaacatcc tgaacaaact ccgtcaaggc acatcccgcc acatcagcct gcatcccggc
     9661 atcagcccca tcacatccgc cgattggctc aaccgcttcg gacacagcct gcacaccaaa
     9721 accgccgtcc tacaccacaa ccctgaagaa gaccagcgca aactgttcag cgcaatccgc
     9781 caatttaccc gccatacgcc gctgattctc cacatcacct gccgcacgac agaaaccgac
     9841 cgtgccgtac tgcactgcct cgcccgacac tgcaacttcc tcatcagttt caacgccgac
     9901 gacctcgaag ccgcactgcg cgcccaactg tccgaccttc cacccctgtc ccgactcgac
     9961 atcctgtccg acacgcctgc cgaatggctg aacgcgcacg cgccaaaaac cctcgccctc
    10021 cactttccca accttcccca ccacatccgc aacggacacc tgaccggcac acccacaccc
    10081 tcaatctgcc acgacatcgc ctcacgtcag cttgcccgcc ccgacaccca agccgtcctg
    10141 accatcctca gtccctccgg acacgaggat tacaaaaaaa cagcacgcgc ccttatccgc
    10201 ctgtccgaac agaccgccaa acccctgctc gtcagcagcc ccttttcaga cggcataacc
    10261 catttcgaca cccccactca ggcaatccgc accctttcct accgcaacac cgccgccgcc
    10321 ctgaaacagg cacagctcga cattgcaccg ccgcagccat gccgtctgaa aacaccgcaa
    10381 ccccaaaaca tcaaaaaagc ccttgcagcg gcaaatccct ccctgctcgc cgaagccctg
    10441 cacctccccc cctaccggca cactacccat aacgccgtcc aattccaatt cggcagccac
    10501 cccatttacg gcaatattct gaccgtacgg tacgcagggc aaaccacggc cgcccttccc
    10561 ccgtttacca cacaggacag cctccatatc gcccagtttg ccgaattgga agacccgcaa
    10621 acccttaacc agttccttca catactgacc accctttccg accacaacct gcacatcggc
    10681 aaaatcaccc tcaacctaaa cggcggacaa tacagcagcg atttcatctt aaagacaccc
    10741 gaaacacacg acacgcccaa acgcaagaac acaggcaaag ccgcccaaac cctcgaacat
    10801 gccgccgcaa aaatgcagag tgccgccgca tacctgaaac acaaaaaccc gacagccgcc
    10861 gaatttctcc gccacacaag cgaagccgcc gcagaactgc tcggcagcaa aaccgaaacc
    10921 ggagcagccg tacccaacgt acttgccccc tatcccgcag cacaccccaa aacactgtcc
    10981 ctaaaaaaca acacgaccgt taccattacc ccccttttgc ccgaagacgc agaagccaaa
    11041 cagcagttcg tccgcagcct cggtcccgaa gcacggtaca cacgcttcat gacccacacc
    11101 aacgaactgc ccgcagccac gttggcacgc ctgtgcaacc ccgattacca ctgtgaagcc
    11161 gcatggacgg caaaggatgc cgacagcaac atcgtcgccg tcgtccgcca cagccgcctg
    11221 aaccgcaacg aatgcgaatt cggcatcaca ctggcggaac atatgcgcgg cagcggactg
    11281 gcacagaaaa tgatggaact catcatccaa accgccgcac agcaaggcta ccggactatg
    11341 agtgccgaca ttctcaaaac caataccccc atgatcaaac ttgctgaaaa atcaggattt
    11401 accctcaagg aatcggacac cgaaaaaaac ctgtaccgcg catatctgaa ccttgcggca
    11461 gacaaaacaa cagaaaaaac aaataaaaac ttgcgcaccg accacaaaat aacctaaaat
    11521 cggcagtttc catatatccg attttctcaa aaggactcaa aatggtagtt atccgtttgg
    11581 cacgcggcgg ctcgaaacac cgccccttct acaacgtcat cgttactgac tcacgcagcc
    11641 gccgcgacgg ccgcttcatc gaacgcgtag gcttctacaa ccccgtagcc aacgaaaaac
    11701 aagagcgcgt ccgcctcaat gcagaccgcc tgaaccactg gattgcacaa ggcgcgcaag
    11761 tcagcgactc cgttgcaaaa ctgattaaag aacaaaaagc cgcctaatcc gcatttgccg
    11821 ccatgacaga cactcaaaac cgggtagcca tgggctacat caaaggcgta ttcggcataa
    11881 aaggctggtt gaaaattgcc gccaacaccg aatattccga cagccttttg gactaccccg
    11941 agtggcattt ggtcaaggac ggcaaaacca tcagcgttac ccttgaagcc ggaaaagtcg
    12001 tcaacggcga actccaagtc aaattcgaag gcataaacga ccgcgacttg gcattctcat
    12061 tgcgcggtta caccatcgaa ataccccgtg aagcattcgc cccgacagaa gaagacgaat
    12121 actactggac agacttggtc ggcatgaccg tcgtcaacaa agaccatacc gttttaggca
    12181 aggtaagcaa cctgatggaa accggcgcaa acgacgtatt gatgattgac ggagaacacg
    12241 ggcagattct gattccgttc gtttcccaat atatcgaaac cgtcgatacc ggcagcaaga
    12301 ttattactgc cgactggggt ttggactact gatgcttatc caggcagtta ccattttccc
    12361 cgaaatgttc gacagcatta cccgctacgg cgtaacggga cgcgcgaaca gacagggaat
    12421 ctggcagttt gaagcagtca atccccgaaa gtttgccgac aacagattgg gctacatcga
    12481 cgaccgcccg ttcggcggcg gcccgggaat gattatgatg gctccgccgc ttcatgcagc
    12541 aatagaacac gccaaagcac aatcttccca agctgcaaaa gtcatttacc tcagccccca
    12601 aggaaaaccg ctgacacacc caaaagcggc agaactggca gaacttccgc atctgattct
    12661 gctgtgcgga cgctatgagg gaatagacga aagactgctg caaaccagcg tcgatgaaga
    12721 aatcagcatc ggagacttcg ttgtttccgg cggagagctt cccgccatga tgctgatgga
    12781 tgcggtattg aggctcgtac ccggcgtatt gggcgatatg cagtctgccg aacaagattc
    12841 gttctcaagc ggcattttgg actgccccca ctacaccaaa cccttagaat ttcaaggcat
    12901 ggctgttccg gaagtattac gttccggcaa tcatggcttg atagcggaat ggcggttgga
    12961 acaatcgctg cgccgcacct tggagcgcag acccgatctt ttggaaaagc gcgttttaat
    13021 cccaaaggaa tcccgcctct tagaaaccat ccggcaagag caacgggaaa tccaatcata
    13081 atttaggaaa aaaacaatga acctgattca acagctcgag caagaagaaa ttgcccgcct
    13141 gaataaagaa atccccgaat tcgcaccggg cgacaccgta gtcgtatccg tacgcgtcgt
    13201 ggaaggtacc cgcagccgtc tgcaagccta cgaaggcgtg gttattgccc gtcgcaaccg
    13261 tggtctgaac agcaacttca tcgtccgtaa aatttccagc ggcgaaggtg tggaacgtac
    13321 tttccaactg tactccccta ccgttgagaa aatcgaagtc aaacgccgtg gcgatgtccg
    13381 ccgtgccaaa ctttactatc tgcgcggcct gaccggcaaa gctgcacgca tcaaagaaaa
    13441 actgcctgca cgcaaaggtt gattcaaacc cgttttcccc aatgccgtct gaaccttctg
    13501 acggcatttc ttattgccgc aacatccgca acggaacagc accaagaagc acaccccttc
    13561 cggcacatcc cgaatccccg tccgaaccga acaaaattta cagaccaaac gttatacagt
    13621 atcatatcag ccatataata ataaccgctt taccgctttc cctttcaaaa ccgcaaatca
    13681 tcatgacacc ttccctttta ctatcaggat tgaccttccg cctcgtcctt gccctgattg
    13741 ccgtatccct tttatggggc gtttactttt gggcagtatc cgcatgagca tcattgtcga
    13801 aaacctgacc gtcagctacc gccgccgtcc tgccgtacac cacgttgaca ttacttttga
    13861 agaacatagt atgtgggcgg ttttcggtcc caacggcgca gggaaatcca cctttctcaa
    13921 atccttgatg ggattgcagc ctatcgatac aggcagcatc cggctggatg gattgagccg
    13981 ccaagacatc gcctaccttc cccagcagtc cgatatcgac cgctcccaac ctatgactgt
    14041 tttcgacttg gcggcaatgg ggctttggta tgaaatcggc tttttcaaag ggataaatac
    14101 cgctcaaaaa caacgcgttc acgaatcttt ggaacgcgtc gggatgcggc agtttgccgg
    14161 ccgccaaatc gcccacctct caaacggaca attccaacgc gtcctttttg cccgaatgct
    14221 ggttcaaaat gcaaaattcc tgcttttgga cgaacctttc aacgccgttg atgcacggac
    14281 gacctacgaa cttctcgacg tattgcagaa atgccattgc ggcggacacg ctatcattgc
    14341 cgtactgcac gattacgaac aagtccgtgc ctactttccc agtaccctgc tgctcgcccg
    14401 agaaaaaatt gcggcaggcg caaccgagac cattctgaca gaaccctacc tcgcccaagc
    14461 caacgccaaa atgcagcaac aggaaagccc cgactggtgc gcctcataaa tactgcccga
    14521 aaccacgccc cacaccagac accatgaatc tctacgacct gctccttgcc ccctttgcag
    14581 aattcgactt tatgcgctac gccctcgcat ccgtcttctg cctgtccctc agtgccgcac
    14641 ccgtcggcgt attcctcgtc atgcgccgta tgagcctgat aggcgacgca ttgagccacg
    14701 ccgtcctgcc cggtgccgcc gtcggctaca tgtttgccgg cttgagcctg cccgccatgg
    14761 gtttgggcgg cgtagccgca ggcatgctga tggcactgct tgccggactc gtcagccgct
    14821 tcaccaccct gaaagaagat gccaactttg ctgcctttta tctcagcagc ctcgccatcg
    14881 gcgtagtcct cgtcagcaaa aacgggagca gcgtcgattt gctccacctc cttttcggct
    14941 ctgtacttgc cgtcgatatt cctgccctgc agctcatcgc cgccgtctcc agcctcacgc
    15001 tcattaccct tgccgtcatc taccgcccgc tcgtactcga aagcatcgac cccctgtttc
    15061 tcaaatccgt cggcggcaaa ggcgggcttt ggcacgtcct ctttctcgtc ctggtcgtca
    15121 tgaacctcgt atccggcttt caagccctcg gcacactcat gtccgtcgga ctcatgatgc
    15181 tgccagccat taccgcccgc ctgtgggcga agcacatggg cgcactcatc ctcctatccg
    15241 ttctgacagc cctgctgtgc ggcttgagcg gactgctcat ttcctaccac atcgaaattc
    15301 cctccggtcc cgccatcatc ctctgttgca gcgtccttta tctcttttcc gtcatactcg
    15361 gcaaagaagg cggcattctg accaaatggc tcaaaaacca ccgccaccac accacctgac
    15421 cccgcaagcc gcttgaacaa acaggcagct tgttttacca taacagatgt tacgttatat
    15481 cacaggagaa cagttatgaa acacctcaaa ctcaccctta ttgccgcatt gctaaccacc
    15541 gccgcaactg ccgcccccct gcccgtcgta accagcttca gcattttagg cgacgttgcc
    15601 aagcaaatcg gcggagagcg cgtatccata caaagtttgg tcggagccaa ccaagatact
    15661 catgcctatc acatgaccag tagcgacatt aaaaaaatcc gcagtgcaaa actcgtcctg
    15721 ctcaacggct taggacttga agctgccgat gtacaacgtg ccgtcaaaca gagcaaagta
    15781 tcctatgccg aagcgaccaa aggcatccaa cccctcaaag ccgaagaaga aggcggacac
    15841 catcatgacc acgaccatga ccacgaagga caccaccatg accacggcga atttgacccg
    15901 cacgtctgga acgaccccgt ccttatgtcc gcctatgccc aaaacgtcgc cgaagccctg
    15961 ataaaggccg accccgaagg caaagtttat tatcaacaac gcttgggcaa ctaccaaatg
    16021 cagcttaaaa aactgcacag cgacgcacaa gccgcattta acgccgtccc tgccgccaaa
    16081 cgcaaagtcc tgaccgggca cgatgccttt tcctacatgg gcaaacgtta ccatattgaa
    16141 ttcatcgccc cgcaaggtgt gagcagcgaa gccgagcctt cagccaaaca agtcgccgcc
    16201 atcatccgac aaatcaaacg cgaaggcatc aaagccgtct ttaccgaaaa catcaaggac
    16261 acccgtatgg ttgaccgtat cgccaaagaa accggtgtca acgtcagcgg caaactgtat
    16321 tccgacgcac tcggcaacgc gcccgcagac acctacatcg gcatgtaccg ccacaacatc
    16381 aaagccttga tcaacgcgat gaagcaataa aaccgtttga ttggtttgaa tttgaaaagg
    16441 caaaaggccg tctgaaaatt atagtggatt aacaaaaatc aggacaaggc gacgaagccg
    16501 cagacagtac agatagtaca gtaaggcgag gcaacgctgt actggttttt gtgaatccac
    16561 tatattgcta gaagtgcacc gattaaggtt aattaaaata cgacccttat cccaatatta
    16621 agaaaaacag caatcaaaat aaacttgatt gctgtttttc agtttaagcc atgaatgtga
    16681 ttaggctcct tatgcagatg gcgttaccaa taacggctgc tgtacggatt gtatattggc
    16741 atccacattt cctcctgtcg cagcagtatt agaaccgaac acagacatag actgtaccaa
    16801 attattgcca gcattaatat acttggcaaa atctggatta ctgatagctg catcatcaaa
    16861 gacaaatgta tctacacgat ggttttcacc atagaagaat ccggaaatac gtacgttgtc
    16921 ttgttcagaa gcgctaagca ccaaatcact tccggaacgg ataaaatgaa catctgctgc
    16981 tttaaatcct ttaaagtgca tagtgtc
//
LOCUS       DAXHSF010000025        16760 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.025, whole genome shotgun sequence.
ACCESSION   DAXHSF010000025 DAXHSF010000000
VERSION     DAXHSF010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16760
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.025"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(124..1293)
                     /gene="metK"
                     /locus_tag="ACFM8S_000672"
     CDS             complement(124..1293)
                     /gene="metK"
                     /locus_tag="ACFM8S_000672"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFC6313779.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAET
                     LVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPD
                     IAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGRL
                     PWLRPDAKAQLTVVYDSETGKVKRIDTVVLSTQHDPSIAYEKLKNAVIEQIIKPVLPS
                     ELLTDETKYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVD
                     RSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLITLV
                     REHFDLRPKGIVQMLDLLRPIYSKSAAYGHFGREEPEFTWERTDKAAALRAAAGL"
     regulatory      complement(1476..1586)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM-I-IV-variant riboswitch; SAM riboswitches
                     modulate the expression of downstream genes in response to
                     levels of S-adenosylmethionine; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            1639..2535
                     /locus_tag="ACFM8S_000673"
     CDS             1639..2535
                     /locus_tag="ACFM8S_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFC6313780.1"
                     /translation="MFRLQFGLFPPLRTAMHILLTALLKCLSLLPLSCLHTLGNRLGH
                     LAFYLLKEDRARIVANMRQAGMNPDPKTVKAVFAETAKGGLELAPAFFRKPEDIETMF
                     KAVHGWEHVQQALDKHEGLLFITPHIGSYDLGGRYISQQLPFPLTAMYKPPKIKAIDK
                     IMQAGRVRGKGKTAPTSIQGVKQIIKALRSGEATIVLPDHVPSPQEGGEGVWVDFFGK
                     PAYTMTLAAKLAHVKGVKTLFFCCERLPDGQGFDLHIRPVQGELNGDKAHDAAVFNRN
                     AEYWIRRFPTQYLFMYNRYKMP"
     gene            complement(2579..3643)
                     /gene="tsaD"
                     /locus_tag="ACFM8S_000674"
     CDS             complement(2579..3643)
                     /gene="tsaD"
                     /locus_tag="ACFM8S_000674"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFC6313781.1"
                     /translation="MLVLGIESSCDETGVALYDTERGLRAHCLHTQMAMHAEYGGVVP
                     ELASRDHIRRLVPLTEGCLAQAGASYGDIDAVAFTQGPGLGGALLAGSSYANALALAL
                     DKPVIPVHHLEGHLLSPLLAEEKPDFPFVALLVSGGHTQIMAVRGIGDYALLGESVDD
                     AAGEAFDKTAKLLGLPYPGGAKLSELAESGRFEAFVFPRPMIHSDDLQMSFSGLKTAV
                     LTAVEKVRAENGADDIPEQTRNDICRAFQDAVVDVLAAKVKKALLQTGFRTVVVAGGV
                     GANRKLRETFGNMTVQIPTPKGKPKHPSEKVSVFFPPTAYCTDNGAMIAFAGAMHLGK
                     GREVGAFNVRPRWPLSEIVK"
     gene            3650..3775
                     /locus_tag="ACFM8S_000675"
     CDS             3650..3775
                     /locus_tag="ACFM8S_000675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313782.1"
                     /translation="MPLKTEKRILADFGTTAVSQKRNMPSEDRSDGIVAPYQSVP"
     gene            complement(3760..4947)
                     /gene="ccsB"
                     /locus_tag="ACFM8S_000676"
     CDS             complement(3760..4947)
                     /gene="ccsB"
                     /locus_tag="ACFM8S_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HFC6313783.1"
                     /translation="MTEHYKTLPEHELLIQKSLISNLNLWDWVFAVLVFAATVFVQTR
                     SGMHMDIYETVMLWASAGIAVFLGWFFKPMRWFVPLSVLLAYAAVGLYGGNIKSAEIF
                     LLRYFLSSQSAIMWQCAFVFFALFAYISGAVLASVKNVPTNTLLGMGTVFAWVSAVAG
                     FTGLLVRWHESYLLRPDAGHIPVSNLYEVFILFLVITALMYLYYEGKFAIQKLGGFVF
                     GFMAVVVGFVLWYSVSREAHTIQPLIPALQSWWMKIHVPANFIGYGAFCISAMLGIAE
                     LVSLRAEGKGGKLWLPPSALIDEVMYKAIAVGFLFFTIATILGALWAADAWGRYWSWD
                     PKETWAFIVWLNYAVWLHLRLVAGWRGKVLAWWAIIGLFVTAFAFIGVNMFLSGLHSY
                     GTL"
     gene            complement(4940..6892)
                     /locus_tag="ACFM8S_000677"
     CDS             complement(4940..6892)
                     /locus_tag="ACFM8S_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HFC6313784.1"
                     /translation="MRFAVALLSLLGIASVIGTVLQQNQPQTDYLVKFGPFWAQIFGF
                     LGLYDVYASAWFVVIMMFLVVSTSLCLIRNVPPFWREMKSFREKVKEKSLAAMRHSSL
                     LDVKIAPEVAKRYLEVQGFQGKTVSREGGSVLIAAKKGTMNKWGYIFAHVALIVICLG
                     GLIDSNLLLKLGMLTGRIVPDNQAVYAKDFKPESILSASNLSFRGNVNISEGQSADVV
                     FLNADNGILVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKATGEKLERTIRVNH
                     PLTLHGITIYQASFADGGSDLTFKAWNLGDASREPVVLKATSIHQFPLEIGKHKYRLE
                     FDQFTSMNVEDMSEGAEREKSLKSTLNDVRAVTQEGKKYTNIGPSIVYRIRDAAGQAV
                     EYKNYMLPVLQEQDYFWITGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGEGR
                     KRLVADATKGAPAEIREQFMLAAENTLNIFAQKGYLGLDEFITSNIPKEQQDKMQGYF
                     YEMLYGVMNAALDETIRRYGLPEWQQDEARNRFLLHSMDAYTGLTEYPAPMLLQLDGF
                     SEVRSSGLQMTRSPGALLVYLGSVLLVLGTVLMFYVREKRAWVLFSDGKIRFAMSSAR
                     SERDLQKEFPKHVESLQRLGKDLNHD"
     gene            complement(7169..7792)
                     /locus_tag="ACFM8S_000678"
     CDS             complement(7169..7792)
                     /locus_tag="ACFM8S_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFC6313785.1"
                     /translation="MKRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGN
                     SGIAMYPRLAAQHTAYIYHQTIGIRDGKRTHGSAAVMKPVVMNLSDQDILNVSAFYAK
                     QQPKSGEANPKENPELGAKIYRGGLSDKKVPACMSCHGPSGAGMPGGGSEIQAYPRLG
                     GQHQAYIVEQMNAYKSGQRKNTIMEDIANRMSEEDLKAVANFIQGLR"
     gene            7998..8627
                     /gene="yihA"
                     /locus_tag="ACFM8S_000679"
     CDS             7998..8627
                     /gene="yihA"
                     /locus_tag="ACFM8S_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFC6313786.1"
                     /translation="MNLFQNAKFFTTVNHLKDLPDTPLEIAFVGRSNAGKSSAINTLT
                     NHVRLAYVSKTPGRTQHINFFELQNGNFMVDLPGYGYAQVPEAVRAHWVNLLGDYLQQ
                     RKQLIGLVLIMDARHPLKELDIRMLDFFHTTGRPVHILLSKADKLSKNEQIKTLSQVK
                     KLLKPYSDRQNISVQLFSNLKKQGIDEANRTVGSWFDAADAAASSPEEN"
     gene            complement(8748..11144)
                     /locus_tag="ACFM8S_000680"
     CDS             complement(8748..11144)
                     /locus_tag="ACFM8S_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HFC6313787.1"
                     /translation="MIKKILTTCFGLVFGFCVFGVGLVAIAILVTYPKLPSLDSLQHY
                     QPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWG
                     VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDK
                     ILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPE
                     RAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVRKIDQSALYVAEMVRQELYE
                     KYGEDAYTQGFKVYTTVRADHQKVATEALRKALRNFDRGSSYRGAENYIDLSKSEDVE
                     ETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVTLDRRALGFAARAVNNEK
                     MGEDRIRRGAVIRVKNNGGRWAVVQEPLLQGALVSLDAKTGAVRALVGGYDFHSKTFN
                     RAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPKNSDGRY
                     SGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRSSELPASLSMALGTGETT
                     PLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLVAGQNAPQAIDPRNAYIM
                     YKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
                     SMGRVGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLT
                     LDNSGIAPQPSRRAKEDDGGAAEGGRQAADDEVRQDMQETPVLPSNTGSKQQQLDSLF
                     "
     gene            11296..12411
                     /gene="pilM"
                     /locus_tag="ACFM8S_000681"
     CDS             11296..12411
                     /gene="pilM"
                     /locus_tag="ACFM8S_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFC6313788.1"
                     /translation="MRLFKSLKNPKKTDAKLPKKSSGLNNRAAIGIDIDQHSIKMVQL
                     SGRSLNQIQLEKYVIAKLPKNIIQGNKVQNYDQLVTYLQQAYAKLGTSCKNIIASVPQ
                     NLATIEQLTYTDKDAELDLQGFVESSISEVSSISLEEANYDYQVLSQSAAGEAVLAVA
                     SRKDEIEPLIDAFNAAGMKLSALDVDIFGQYNAYALWINHFAPELAAEKVAIFGVYAA
                     QTYALVIQDGKILYKQETSVSEEQLNQLIQRTYQVTEEKAEEIINSPQKPSDYQESVA
                     NYFNQQITQEIQRVLQFYYTTQTADDMTDIKHILLTGEAARQEGIAQTVASQTNADVQ
                     CVHPARYFADNLKTDKQQFELDAPTLTRAFGLAVRGL"
     gene            12414..13013
                     /locus_tag="ACFM8S_000682"
     CDS             12414..13013
                     /locus_tag="ACFM8S_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HFC6313789.1"
                     /translation="MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYL
                     FIDNMINNQSERNTLLETSIAHLDTELSEIQKLKQEKDAFLIKKNKIEELQLKRLQAA
                     KILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
                     SIKKNNSHQEFTLQATLQPIVKAAESKENPASGNAQEAN"
     gene            13014..13661
                     /locus_tag="ACFM8S_000683"
     CDS             13014..13661
                     /locus_tag="ACFM8S_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HFC6313790.1"
                     /translation="MASKSSKTNLDLNNLHLLNLPARLFIALLAVAAVLGLGYAGLFK
                     SQMESLEEYEAKETELKNTYKQKSIDAASLNNLRDELASIRSAFDIMLKQLPTDAEIP
                     NLVQELHQAGSSNGLRLDSVMPQPPVDDGPIKRLPYSISITGNYEQISQFTRDVGSLS
                     RIITLESLKIAQSPENGGNPDGKSSILNLSAIATTYQAKSVEELAAEAAQNAEQK"
     gene            13679..14224
                     /locus_tag="ACFM8S_000684"
     CDS             13679..14224
                     /locus_tag="ACFM8S_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HFC6313791.1"
                     /translation="MKHYALLISFLALSACSQGSEDLNEWMAQTRREAKAEIIPFQAP
                     TLPVAPVYSPPQLTGPNAFDFRRMETDKKGENAPDTKRIKETLEKFSLENMRYVGILK
                     SGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIVLNELIEDSTGNWVSRKAE
                     LLLNSSDKNTEQAAAPAAEQN"
     gene            14243..16528
                     /gene="pilQ"
                     /locus_tag="ACFM8S_000685"
     CDS             14243..16528
                     /gene="pilQ"
                     /locus_tag="ACFM8S_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HFC6313792.1"
                     /translation="MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKV
                     SFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLV
                     LNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPSTKSAV
                     SVSKPFTPAKQQAAAPFTESVVSVSAPFSPAKQQAAASAKQQTAAPAKQQAAAPAKQT
                     NIDFRKDGKNAGIIELAALGFAGQPDISQQHDHIIVTLKNHTLPTTLQRSLDVADFKT
                     PVQKVTLKRLNNDTQLIITTAGNWELVNKSAAPGYFTFQVLPKKQNLESGGVNNAPKT
                     FTGRKISLDFQDVEIRTILQILAKESGMNIVASDSVNGKMTLSLKDVPWDQALDLVMQ
                     ARNLDMRQQGNIVNIAPRDELLAKDKAFLQAEKDIADLGALYSQNFQLKYKNVEEFRS
                     ILRLDNADTTGNRNTLVSGRGSVLIDPATNTLIVTDTRSVIEKFRKLIDELDVPAQQV
                     MIEARIVEAADGFSRDLGVKFGATGKKKLKNDTSAFGWGVNSGFGGDDKWGAETKINL
                     PITAAANSISLVRAISSGALNLELSASESLSKTKTLANPRVLTQNRKEAKIESGYEIP
                     FTVTSIANGGSSTNTELKKAVLGLTVTPNITPDGQIIMTVKINKDSPAQCASGNQTIL
                     CISTKNLNTQAMVENGGTLIVGGIYEEDNGNTLTKVPLLGDIPVIGNLFKTRGKKTDR
                     RELLIFITPRIMGTAGNSLRY"
BASE COUNT         4507 a         4771 c         3818 g         3664 t
ORIGIN      
        1 ttttgcaaag gtctctatct gtactgtctg cggcttcgtc gccttgtcct gatttttgtt
       61 aatccactat ataaaaatgc cgtctgaact gttcagacgg catttttgat tttcaaaccg
      121 gaattacagc cccgctgccg ccctcaatgc agcagctttg tcggtgcgct cccaagtgaa
      181 ctcaggttct tcgcggccga aatgtccgta agcggcggat ttactgtaaa tcgggcgcaa
      241 gaggtcgagc atttggacga tgcctttggg gcgcaggtcg aaatgttcgc gaactaaggt
      301 aatcagtttt tcttcgctga ttttgcctgt gccgaaagta tcgatggaaa tcgaagtcgg
      361 ttcggcaacg ccgatggcgt aggaaacttg gatttggcat tgggttgcca atcctgcggc
      421 gacgatgttt ttcgccacat agcggcaggc gtaggcggcg gaacggtcaa ccttggacgg
      481 gtctttgccg gaaaacgcgc cgccgccgtg cggagccgcg ccgccgtagg tatcgacgat
      541 gattttacgg ccggtcaaac cgcagtcgcc ttgcgggccg ccgatgacga agcggccggt
      601 cgggttgatc aggtatttgg tttcgtcggt cagcagttca gacggcagga cgggcttgat
      661 aatctgctcg atgacggcgt ttttcagttt ttcgtaagcg atggacggat cgtgctgggt
      721 agagaggacg acggtgtcga tgcgttttac tttgccggtt tcgctgtcgt aaaccacggt
      781 cagttgggct tttgcgtcgg ggcgcagcca aggcaggcgg ccgtctttgc gtaattcgct
      841 ttgacgctgc atcaggcggt ggctgtaata gatggcaaac ggcatcaggg tcggggtttc
      901 gtcgcaggca tagccgaaca tcaaaccttg gtcgcccgcg ccctggttca ggtcgatgcc
      961 ttcgccttcg ttcacgcctt gggcgatgtc gggggattgc tggtcgtagt acacgccgac
     1021 cgcgcagccg ttggcatcaa agcccagctc ggacgagttg tagccgatgc gtttgatggt
     1081 ttcgcgtgcg actttgatgt agtccacttg ggcggtggtg gtgatttcgc ccgccaatac
     1141 gcacaagcct gtgttgacca aggtttctgc agcgacacgc gctttggggt cttgcgccaa
     1201 gatggcatcc aaaatcgcat cggacacttg gtcggcaact ttgtccggat ggccttcgga
     1261 taccgattcg gaagtaaaca gatattcgct cattgagatt ttccttgaaa aacaaaccat
     1321 cttcttcaga cggcatgttg tatgaacata atgtcgacag cgggaaatat agcaaaattt
     1381 ccctattcat accattcagt tgagaaatat tcccatttga atggcacttt ggaatctctg
     1441 cccgtacgtt tcttacaggc aaaaaaattc ccgcatcaag cgggtttgga ttgctctggt
     1501 gagccacatc ggcttttcaa ccgtccacct tacttttcct tttgaaaagc aggttggcat
     1561 ggaattccca actcttaatg cagcgcggat ggtagcagaa aagcctttcc gccgcaatac
     1621 ttcccttttt cagacggcat gtttcgttta caattcgggc tgtttccccc tttgcgaacc
     1681 gccatgcaca tcctgttgac cgccctgctc aaatgcctct ccctgctgcc actttcctgt
     1741 ctgcacacgc tgggaaaccg gctcggacat ctggcgtttt accttttaaa ggaagaccgc
     1801 gcgcgcatcg tcgccaatat gcgtcaggca ggcatgaatc ccgaccccaa aacagtcaaa
     1861 gccgtttttg cggaaacggc aaaaggcggt ttggaacttg cccccgcgtt tttcagaaaa
     1921 ccggaagaca tagaaacaat gttcaaagcg gtacacggct gggaacatgt gcagcaggct
     1981 ttggacaaac acgaagggct gctattcatc acgccgcaca tcggcagcta cgatttgggc
     2041 ggacgctaca tcagccagca gcttccgttc ccgctgaccg ccatgtacaa accgccgaaa
     2101 atcaaagcga tagacaaaat catgcaggcg ggcagggttc gcggcaaagg aaaaaccgcg
     2161 cctaccagca tacaaggggt caaacaaatc atcaaagccc tgcgttcggg cgaagcaacc
     2221 atcgtcctgc ccgaccacgt cccctcccct caagaaggcg gggaaggcgt atgggtggat
     2281 ttcttcggca aacctgccta taccatgacg ctggcggcaa aattggcaca cgtcaaaggc
     2341 gtgaaaaccc tgtttttctg ctgcgaacgc ctgcctgacg ggcagggttt cgatttgcac
     2401 atccgccccg tccaagggga attgaacggc gacaaagccc atgatgccgc cgtgttcaac
     2461 cgcaatgccg aatattggat acgccgtttt ccgacgcagt atctgtttat gtacaaccgc
     2521 tacaaaatgc cgtaacgaaa ataaaaatgc cgtctgaaca atttcagacg gcattttgtc
     2581 atttgacgat ttccgacagc ggccagcgcg gacggacatt gaacgcaccg acctccctgc
     2641 ccttgcccag gtgcatcgca ccggcaaagg caatcatggc accgttgtcc gtgcagtatg
     2701 ccgtcggcgg gaaaaacacg ctgacttttt cggacggatg tttcggcttg cctttggggg
     2761 tcgggatttg caccgtcatg ttgccgaaag tttcacggag cttgcggttt gcaccgaccc
     2821 cgccggcgac cactacggtt ctgaaccctg tctgcaacag ggcttttttc actttcgccg
     2881 ccaacacatc gactaccgca tcttgaaacg cacggcagat gtcgttgcgt gtctgctcag
     2941 gaatgtcatc cgccccgttt tccgcgcgca ctttctcgac ggcggtcaat acggcggttt
     3001 tcaaacctga aaaactcatc tgcaaatcgt cggaatgaat catcgggcgc ggaaaaacaa
     3061 acgcttcgaa cctgcccgat tccgcaagtt ccgacagttt cgcaccgccc ggatacggca
     3121 agcccagcag tttcgccgtt ttgtcgaatg cctcgcccgc cgcatcatcg acgctctcgc
     3181 ccaaaagcgc gtagtcgcct atgcccctga ccgccataat ctgcgtatgc ccgcccgaaa
     3241 ccaacagcgc gacaaaagga aagtcgggtt tttcctccgc caacagcggc gacagcagat
     3301 gtccttccaa atgatggacg ggaataacag gcttgtccaa cgctaaagcc agcgcgttgg
     3361 cgtagctcga acccgccagc agcgcaccgc ccaaaccggg cccctgcgta aaggcaaccg
     3421 cgtcaatgtc gccatacgat gcgcccgcct gcgccaaaca gccttcagtc aacggaacaa
     3481 ggcggcggat atggtcgcgg cttgccagct ccggcacaac cccgccgtat tcggcgtgca
     3541 ttgccatctg cgtgtgcagg cagtgcgccc gcaatcctcg ttcggtgtca tacagcgcaa
     3601 cgcctgtttc gtcgcaagaa gattcgattc ccaataccaa catggtctga tgccgttaaa
     3661 aactgaaaaa cgtattttag cggatttcgg cacgactgcc gtatcccaaa aacggaacat
     3721 gccgtctgaa gaccgttcag acggcatcgt cgcaccgtat caaagcgttc cgtaagaatg
     3781 cagcccgctc aaaaacatat tcacgccgat aaaggcaaat gcggttacga acaaaccgat
     3841 aatcgcccac cacgccagca ctttgccgcg ccaaccggca accagccgca agtgcagcca
     3901 aacggcgtaa ttgagccaga cgatgaacgc ccacgtctct ttcggatccc aactccaata
     3961 gcgtccccaa gcatctgccg cccacagcgc acccaaaatg gtggcaatgg taaagaacag
     4021 aaagccgacg gcaatcgcct tatacatcac ctcgtcgatc aatgccgacg gcggcagcca
     4081 cagttttccg ccttttcctt ccgcacgcag ggaaaccagt tcggcaatac cgagcatcgc
     4141 ggaaatgcaa aacgcgccgt aaccgataaa gtttgccgga acgtggattt tcatccacca
     4201 ggactggagc gcgggaatca gcggctggat ggtatgcgcc tcgcgggaca cgctgtacca
     4261 caagacaaat ccaaccacga ccgccataaa gccgaacacg aagccgccca atttctgtat
     4321 ggcgaactta ccttcataat aaagatacat cagcgcggta atcaccaaaa acaggatgaa
     4381 cacttcatac aggttggaaa ccggaatatg ccccgcatcg ggacggagca gatagctttc
     4441 gtgccaacgt accagcagac cggtaaagcc tgctacggca gacacccatg caaacacggt
     4501 tcccataccc aacagcgtgt tggtcggcac attttttacg cttgccaaaa ccgcgcccga
     4561 aatataggcg aacagggcga agaagacaaa ggcgcactgc cacatgatcg ccgactggct
     4621 gctgaggaaa taccgcaaca ggaaaatctc tgccgatttg atgttgccgc catacaaacc
     4681 gacggcggca taggcaagca atacgcttaa aggaacaaac cagcgcatcg gtttgaaaaa
     4741 ccaacccaaa aacacggcaa taccggcact tgcccacaac atgaccgttt cgtaaatgtc
     4801 catatgcata ccggaacggg tctgtacgaa aaccgtagcc gcaaaaacca gcacggcaaa
     4861 tacccaatcc caaagattca gattgctgat caaagacttc tgaatcagca gctcgtgttc
     4921 cggaagggtt ttatagtgtt cagtcatgat tcaagtcctt gccgagccgt tgcagactct
     4981 cgacgtgttt tggaaattcc ttctgcaaat cccgttcgct gcgggccgaa gacatggcaa
     5041 aacggatttt gccgtctgaa aacaataccc acgcccgttt ttcgcgcaca taaaacatca
     5101 ataccgtacc caataccaac agcaccgagc cgagatagac caaaagcgca cccggggaac
     5161 gggtcatctg caaacccgac gaacgcacct cggaaaaccc atcaagttgc agcagcatag
     5221 gcgcgggata ttcggtcaaa cccgtgtacg catccatact gtgcagcagg aaacgattcc
     5281 gcgcttcatc ctgctgccat tcgggcaagc cgtaccggcg tatggtttca tccaaagcag
     5341 cgttcatcac gccgtaaagc atttcgtaga aatagccctg catcttatcc tgctgctctt
     5401 tcgggatatt ggacgtaata aattcgtcca atcccaaata gcctttttgt gcaaagatgt
     5461 tcagcgtgtt ttccgcagcc agcatgaatt gttcgcggat ttcggcaggt gcgcctttgg
     5521 ttgcgtcggc aaccagacgt ttgcgccctt ccccatcttt caaaaactca cgcaatgcca
     5581 taaaggtgtc cgctttcaac tgcttgtcca aggggatacg cagccagcgg tattgctgct
     5641 gcaagccgct gcgcgtgccg gtaatccaaa aataatcctg ttcctgcaaa accggcagca
     5701 tatagttttt atattcgacc gcctgccctg ccgcatcacg gatacggtaa acaatggaag
     5761 ggccgatatt ggtgtatttt ttaccttcct gagtaacggc gcggacatcg ttcagcgtgg
     5821 atttcaggct tttttcccgt tccgcgccct cgctcatgtc ctccacattc atagaagtaa
     5881 actgatcgaa ctcaagacga tatttgtgtt tgccaatttc caacggaaac tggtgtatgg
     5941 atgttgcctt caacacgaca ggctcgcgcg aagcatcacc caaattccac gccttgaatg
     6001 tcaaatccga accgccgtcg gcaaaactcg cctgataaat cgtgatgccg tgcaaggtca
     6061 aaggatggtt cacgcggatg gtgcgctcga gtttctcacc ggttgcctta tccgttactt
     6121 caatatcact ggcaaaatcg cgcggcatac ccgtattgta aaaatcgata tggaattttt
     6181 tcagtttgac ttcaaaaggc aagtcctgaa ccaatatccc gttgtcggca ttcaggaaaa
     6241 ccacatccgc actctgcccc tcggaaatat tgacgttgcc cctaaatgag agattggacg
     6301 cactcaaaat actttcgggc ttgaaatcct tggcataaac cgcctgattg tccggaacaa
     6361 tccgaccggt cagcataccc agtttcaaca gcaggttact gtctatcaac ccgcccaggc
     6421 aaatgacaat caaagcaaca tgggcaaaga tatagcccca tttgttcatc gtgccttttt
     6481 tggcggcaat cagaaccgac ccgccctcac ggctgacggt ttttccctga aaaccctgca
     6541 cctccagata acgtttggca acctcgggcg caatttttac atccaacagc gaagaatggc
     6601 gcatcgccgc cagagatttt tctttaacct tttcccgaaa agacttcatt tcgcgccaga
     6661 acggcggcac attgcgaatc aggcacaaac tggtagaaac caccaaaaac atcatgataa
     6721 cgacaaacca tgccgaagca tagacgtcat acagtcccag aaaaccaaaa atctgcgccc
     6781 aaaaaggtcc gaatttgacc aaataatccg tctgcggctg gttttgctgc aacaccgtac
     6841 cgataaccga tgcaataccc agcagactga gcaaagcgac tgcaaagcgc atggagctga
     6901 aaaaagcgaa ccacggacgg gaaagaagtg ggggagatct acgggattta ctcattgtgt
     6961 gtttattccg ccatcaggaa tatgggaaag cagaattggg caaacagaaa acaacgtccc
     7021 gattctactg tcttgatgct ttttatttca agacaatgaa gacagcctgc atcgattcca
     7081 acggttgcga ttgaaaaaac ttatcgcaga attgcctgaa gccgtctgaa aacttttcag
     7141 acggcctcta aaacagacta ttgcggaatt aacgcaaacc ttggataaag ttggcgaccg
     7201 ctttcaaatc ttcttcagac atacggtttg caatatcttc catgatggta tttttacgct
     7261 gaccggactt gtaggcattc atctgttcaa caatatatgc ctgatgctga ccgcccaaac
     7321 gcggataagc ctgaatttcg cttccgcctc ccggcatacc cgcaccgctc ggaccgtggc
     7381 aggacataca cgccggcact tttttatcgc tcaaaccgcc gcgatagatt ttcgcaccca
     7441 attcgggatt ttccttagga ttggcttcac cggatttggg ctgctgtttg gcatagaatg
     7501 cggatacgtt caaaatatcc tgatcgctca aattcattac caccggtttc atcacagctg
     7561 ccgaaccgtg ggtacgtttg ccgtcgcgga taccgatagt ttgatgatag atgtaagcag
     7621 tatgctgtgc cgccaaacgt ggatacatcg caatgccgct attaccgtct gctgcatggc
     7681 aagccgcaca aaccgttgcg gcaacctgtt tgcctttttc cacgtctgct ttgggagatg
     7741 cggaaaccgc accggcagcc aaaacaaagg ccaataaagt caatcgtttc atggagtgct
     7801 cctgattaca gcattggata acgcaacaat gctcttttta tattcaaata cgggattttt
     7861 gacccgatta aaaccgatga ttctgtaaac gtgttattct atactaaatt tacattaaat
     7921 taccactgtg tttcacataa aaccaaccgc atatttttgc tgtcggacaa acggcggcgg
     7981 aaaacaagga tatgcccatg aacctttttc aaaacgccaa attcttcacg acggtaaacc
     8041 atctcaaaga cctgcccgac acccctctcg aaattgcctt tgtcggcagg agcaatgccg
     8101 gaaaatccag tgccatcaat accctgacca accatgtccg tcttgcctac gtttcaaaaa
     8161 cacccggacg gacgcagcat atcaacttct tcgagctgca gaacggcaat tttatggtcg
     8221 atttgcccgg ctacggttat gcccaagtcc ctgaagcagt acgcgcacat tgggtcaatc
     8281 tgctcggcga ctatctgcaa cagcgcaaac agcttatcgg gctggttttg attatggatg
     8341 cccgccatcc tttaaaagaa ctcgacatcc gtatgctgga ttttttccac acgaccggca
     8401 gaccggttca catcctgctg tcaaaagccg acaaattatc caaaaacgaa cagataaaaa
     8461 ccctgtccca agtcaaaaaa ctgctcaaac cttattccga caggcaaaac atcagcgtac
     8521 agctgttttc caacctgaaa aaacaaggta ttgacgaggc caaccgaact gtcggaagct
     8581 ggtttgacgc agcagatgcc gccgcttcct ctccagagga aaactgaccc caattatacg
     8641 gaaaccgtat ttcccccact tgaccgaccg caaacattta aaaaattgcc actgccaaat
     8701 ctaaaatgcc gtctgaaaga cttttcagac ggcattttgc ggagtcttta aaacagagaa
     8761 tccaactgct gctgtttgga accagtatta ctcggaagca ccggcgtttc ctgcatatct
     8821 tggcggactt cgtcatccgc cgcctgccgt ccgccttctg ccgcgccccc gtcatcttct
     8881 tttgcccgtc gggaaggttg cggcgcaata ccgctgttgt ccagcgtcaa gcccggatcg
     8941 gttaccatac gttccttcat atagtattcg ccattgctgc tgaccacacc ttcaggcatt
     9001 ttcatcccct tgccctgctt tcctttcaac gcaaaacgca tatagtccac ccaaaccggc
     9061 accgcaatcg taccgccgta gccgacacgc cccatactct taggtttgtc gaagccgata
     9121 tatacggcag taaccacatc agggttaaaa ccgacaaacc acgcatcctt attgtcattg
     9181 gtcgtacccg ttttaccggc aatatccgtt cttcccaacg cagctgcccc ccttgccgta
     9241 ccgacacgga ccacatcctg cataatctta tacataatat aggcattgcg cggatcgatt
     9301 gcctgaggcg cattttgccc agccaccaaa ggttgcattt gggcgcgcaa cctgccgtct
     9361 ctgtcataaa tcttatcgat tacgtgcgaa gaaaccctat atccgccgtt cgcaaatacg
     9421 ctatatgcct ccgccacttt caacggcgtt gtctcgcccg tacctaaagc catagacagg
     9481 cttgccggca gctcggacga cctgaagccg aaacgccgga tatactgttg cgcgtaaccg
     9541 acaccgatag acatcaaaat acggatggaa accatattct tggaagccgt cagagcctgt
     9601 ctcaaagtaa tgtagccgga atatctgccg tctgaatttt taggtgtcca aaccgaaccg
     9661 ttcggccctt tccccggcag ggaaatcggc gcatcgttaa ccactgtgga cgcggtcatc
     9721 cccttagata atgccgccga atagacaaac ggcttaaagg tcgaacccgg ctgccgcatt
     9781 gcctgaacgg cacgattgaa tgttttgctg tgaaaatcat aaccgccgac cagcgcgcgc
     9841 acagctccgg tttttgcatc cagcgaaacc aaagccccct gcagcaacgg ctcttgaacc
     9901 accgcccaac gcccgccgtt gtttttgaca cggatgaccg cgcccctgcg gatacggtcc
     9961 tcccccattt tttcattatt gaccgcgcgg gccgcaaagc ccaaggcgcg cctatcaagc
    10021 gtaacccgcc tgccgccggg cagctgtatg acgacatttt tctttttagt cacatccaac
    10081 acaacggcgg gaaccatttt atcgacggta tagagtcccg acagatactg gctgacagtc
    10141 tcctcgacat cttcactctt actcaaatcg atatagtttt ccgcaccgcg gtagctgctg
    10201 ccgcgatcga aattccgtag agccttgcgc aatgcctcgg ttgccacctt ctgatgatcg
    10261 gcgcggaccg tggtataaac cttaaaaccc tgcgtatagg catcttcacc gtatttctca
    10321 tacagttcct gacgcaccat ttccgccaca tataaagcac tctgatcgat tttccgaaca
    10381 aaccgctcgt aatgcagttc ctcattcaac gcctgatcgc gctgttgcac ggtaatcatc
    10441 ttctcctcga gcatattgtt caaaatatac ttctggcgca acttggcacg ttctggatta
    10501 acaatcggat tataggcaga cggagccttg ggcagtcccg caagcatggc ggcttccgcc
    10561 aaagtcaaat ctcggacatt cttattgaaa tagatttgcg cggcagatgc aaaaccatag
    10621 gcgcgctgac cgaggtaaat ctgattgaaa tacaactcga ggattttgtc tttgcttaaa
    10681 gactgctcga ttttataggc aagcaacacc tcattgaatt tgcgtgtgaa cgttttttca
    10741 ctgctcaaat aaaaattttt cgccacctgc tgcgtaatcg tactcgcacc cgactgcacg
    10801 ctgccggaca cgacattgcc gacggcagcg cgggcaacac cccaaacatc caccccccaa
    10861 tgccggtaaa agcgtttgtc ctcggcggcg ataaccgcat tccgcaacac ctctgggaaa
    10921 tcgccgattt ttgtaaattc gcgccgctgc tccccataca taccgatgac ttccccatcc
    10981 gccgaataaa tagtcaacgg cattttaggc tggtaatgct gcaaagaatc caaagacggc
    11041 agtttcggat acgttaccaa aatagcaatg gcaaccaaac ccactccaaa tacacaaaac
    11101 ccaaaaacca aaccaaaaca agtcgttaaa atctttttaa tcatagctga ataataattt
    11161 accattattg gtattaaata aagtaaaata gcaaccgatt tctacaaagc acggtttcaa
    11221 tgtgcaaaga acaaggaatc cattacggat accgaaacgg ttactcactg tacaaataaa
    11281 gcaggaactt tcatcatgcg cttgtttaaa agcttgaaaa accctaaaaa aacagatgcc
    11341 aagctcccta aaaaatcttc gggactcaat aaccgcgcgg caatcggcat cgatatcgac
    11401 cagcattcca tcaaaatggt ccaattgtca ggacgtagtt taaaccaaat tcaattggaa
    11461 aaatacgtca ttgccaaatt accaaagaat atcattcaag gcaataaagt ccaaaattac
    11521 gatcaacttg ttacatattt gcaacaagcc tatgccaaac tgggtacttc gtgcaaaaac
    11581 atcatcgcgt ccgtcccgca aaatctggca accatcgaac aattgaccta cacagacaaa
    11641 gatgcagaat tagacctgca ggggttcgtg gagtcctcca tctccgaagt cagctcgata
    11701 tcgctcgaag aagccaatta tgactatcag gtcttgtccc aatcggccgc cggcgaagct
    11761 gtgttggccg tcgcatcgag aaaggatgaa atcgaacccc tgattgacgc attcaacgcc
    11821 gccggtatga aattatccgc gcttgatgtg gacattttcg gacaatacaa cgcctacgcg
    11881 ctatggataa accatttcgc ccccgagctt gcagccgaaa aagtcgccat tttcggcgta
    11941 tatgccgcac agacctacgc cttggtcatc caagacggaa aaatcctata caaacaggaa
    12001 acctccgtca gcgaagaaca gctcaaccaa ctcatccagc gcacctatca ggtaacagaa
    12061 gaaaaagcgg aagaaatcat caactccccg caaaaacctt ccgattacca agaaagcgtg
    12121 gcaaactatt tcaaccagca gattacccaa gaaatacaaa gggtcttgca gttttattac
    12181 accacgcaga ccgcagacga tatgaccgac atcaagcata tcctgctgac cggggaagcg
    12241 gcgcgccagg aaggcatcgc ccaaaccgtc gcctcacaaa ccaatgcaga tgtacaatgc
    12301 gtccatcccg cgcgttattt tgcggacaac ctcaaaacag acaaacaaca attcgaactt
    12361 gatgcgccga cactgaccag ggcgttcggt ttggcggtac ggggattata attatgaaca
    12421 atttaatcaa aatcaacctc ctcccctaca gggaagagat gaacaagcgc aaacagcagc
    12481 agtttaaaac gctgatgtac ggtgccgtgc tgacgggcgt tgccgccgtt gcggcaacct
    12541 acctgtttat cgacaatatg atcaataacc agtcggaaag aaacacgctg ctggaaacct
    12601 ccatcgcaca cttggatacc gagctgtcgg aaatacaaaa gctcaaacag gaaaaagatg
    12661 ccttcctgat taagaaaaac aaaatcgagg agctccagct caaacgcctc caagccgcaa
    12721 aaatcctcga cagcctgaat gaggccgtcc ccggaagcac ctacctgacc tcgctggatg
    12781 ccgttaccgc cgactcttat cggctcagcg gcaggacatc cagcgacaac cgcgttgccg
    12841 ccatgatgag ggcgatgccc aataccggca tattcaagca acccgaattg ttaagcatca
    12901 agaaaaacaa ttcgcatcaa gaatttaccc ttcaggcaac attacaaccc atcgtaaagg
    12961 cggccgaatc caaagagaat ccggcttcgg gaaacgcaca ggaggcaaac tgaatggctt
    13021 ctaaatcatc taaaaccaac ttggatctca acaaccttca cctgctcaac cttcctgcca
    13081 ggctttttat cgccctgctg gccgttgccg ccgtgctggg gctcggttat gccggattgt
    13141 tcaaaagcca gatggaatcc cttgaggaat acgaagcaaa agaaaccgaa ctgaaaaaca
    13201 cctacaaaca gaaaagtatc gacgcggcca gcctgaacaa cctgagggac gaacttgcct
    13261 caatccgctc tgccttcgat atcatgttga aacagctgcc gacagatgca gaaattccca
    13321 acctggttca agagcttcat caggcaggtt cgagcaacgg cctgcgcttg gacagcgtta
    13381 tgccccaacc tcccgtagat gacggcccca tcaaaagatt accctattcc atttccatta
    13441 ccggaaatta cgaacagatc agccaattta cccgcgatgt cggcagcctc tcccgaatca
    13501 ttacccttga gtcgctgaaa atcgcccaat ctccggaaaa cggcggcaat cctgacggca
    13561 agagcagcat cctgaacctc agtgccattg ccaccaccta ccaagcaaaa tccgtagaag
    13621 agcttgccgc agaagcggca caaaatgccg agcaaaaata acttacgtta gggaaaccat
    13681 gaaacactat gccttactca tcagctttct ggctctctcc gcgtgttccc aaggttctga
    13741 ggacctaaac gaatggatgg cacaaacgcg acgcgaagcc aaagcagaaa tcataccttt
    13801 ccaagcacct accctgccgg ttgcgccggt atacagcccg ccacagctta cagggccgaa
    13861 cgcattcgac ttccgccgca tggaaaccga caaaaaaggg gaaaatgccc ccgacaccaa
    13921 gcgtattaaa gaaacgctgg aaaaattcag tttggaaaat atgcgttatg tcggcatttt
    13981 gaagtctgga cagaaagtct ccggcttcat cgaggctgaa ggttatgtct acactgtcgg
    14041 tgtcggcaac tatttgggac aaaactacgg tagaatcgaa agcattaccg acgacagcat
    14101 cgtcctgaac gagctgatag aagacagcac gggcaactgg gtttcccgta aagcagaact
    14161 gctgttgaat tcttccgaca aaaacaccga acaagcggca gcacctgccg cagaacaaaa
    14221 ttaagaagag gattactcca ttatgaatac caaactgaca aaaatcattt ccggtctctt
    14281 tgtcgcaacc gccgcctttc agacagcatc ggcaggaaac attacagaca tcaaagtttc
    14341 ctccctgccc aacaaacaga aaatcgtcaa agtcagcttt gacaaagaga ttgtcaaccc
    14401 gaccggcttc gtaacctcct caccggcccg catcgccttg gactttgaac aaaccggcat
    14461 ttccatggat caacaggtac tcgaatatgc cgatcccctg ttgagcaaaa tcagtgccgc
    14521 acaaaacagc agccgtgcgc gtctggttct gaatctgaac aaaccgggcc aatacaatac
    14581 cgaagtacgc gggaacaaag tttggatatt cattaacgaa tcggacgata ccgtgtccgc
    14641 ccccgcacgc cccgccgtaa aagccgcgcc tgccgcaccg gcaaaacaac aggctgccgc
    14701 accgtctacc aagtccgcag tatccgtatc caaacccttt accccggcaa aacaacaggc
    14761 tgccgcaccg tttaccgagt ccgtagtatc cgtatccgca ccgttcagcc cggcaaaaca
    14821 acaggcggcg gcatcagcaa aacaacagac ggcagcacca gcaaaacaac aggcggcagc
    14881 accagcaaaa caaaccaata tcgatttccg caaagacggc aaaaatgccg gcattatcga
    14941 attggctgca ttgggctttg ccgggcagcc cgacatcagc caacagcacg accacatcat
    15001 cgttacgctg aaaaaccata ccctgccgac cacgctccaa cgcagtttgg atgtggcaga
    15061 ctttaaaaca ccggttcaaa aggttacgct gaaacgcctc aataacgaca cccagctgat
    15121 tatcacaaca gccggcaact gggaactcgt caacaaatcc gccgcgcccg gatactttac
    15181 cttccaagtc ctgccgaaaa aacaaaacct cgagtcaggc ggcgtgaaca atgcgcccaa
    15241 aaccttcaca ggccggaaaa tctcccttga cttccaagat gtcgaaatcc gcaccatcct
    15301 gcagattttg gcaaaagaat ccgggatgaa cattgttgcc agcgactccg tcaacggcaa
    15361 aatgaccctc tccctcaaag acgtaccttg ggatcaggct ttggatttgg ttatgcaggc
    15421 acgcaacctc gatatgcgcc aacaagggaa catcgtcaac atcgcgcccc gcgacgagct
    15481 gcttgccaaa gacaaagcct tcttacaggc ggaaaaagac attgccgatc tgggcgcgct
    15541 gtattcacaa aacttccaat tgaaatacaa aaatgtggaa gaattccgca gcatcctgcg
    15601 tttggacaat gccgacacaa ccggaaaccg caatacgctt gtcagcggca ggggcagcgt
    15661 gctgatcgat cccgccacca ataccctgat tgttaccgat acccgcagcg tcatcgaaaa
    15721 attccgcaaa ctgattgacg aattggacgt acccgcgcaa caagtgatga ttgaggcgcg
    15781 tatcgtcgaa gcggcagacg gcttctcgcg cgatttgggc gttaaattcg gcgcgacagg
    15841 caagaaaaag ctgaaaaatg atacaagcgc attcggctgg ggggtaaact ccggcttcgg
    15901 cggcgacgat aaatgggggg ccgaaaccaa aatcaacctg ccgattaccg ctgccgcaaa
    15961 cagcatttcg ctggtgcgcg cgatttcctc cggtgccttg aatttggaat tgtccgcatc
    16021 cgaatcgctt tcaaaaacca aaacgcttgc caatccgcgc gtgctgaccc aaaaccgcaa
    16081 agaggccaaa atcgaatccg gttacgaaat tcctttcacc gtaacctcaa tcgcgaacgg
    16141 cggcagcagc acgaacacgg aactcaaaaa agccgtcttg gggctgaccg ttacgccgaa
    16201 catcacgccc gacggccaaa tcattatgac cgtcaaaatc aacaaggact cgcctgcgca
    16261 atgtgcctcc ggtaatcaga cgatcctgtg tatttcgacc aaaaacctga atacgcaggc
    16321 tatggttgaa aacggcggca cattgattgt cggcggtatt tatgaagaag acaacggcaa
    16381 tacgctgacc aaagtccccc tgttgggcga catccccgtt atcggcaacc tctttaaaac
    16441 acgcgggaaa aaaaccgacc gccgcgaact gctgattttc attaccccga ggattatggg
    16501 tacggccggc aacagcctgc gctattgatg cgtcaaaata agggcatatg ttttacggca
    16561 tatgcccttt ctttatgctt tttgccgcga ccgaaatgcc gtcattcccg cgcaggcaga
    16621 aatccaactt gtccggttcc ggttgttttt cgtctcgtaa cttttgagcc gtcattcccg
    16681 cgcaggcaga aatccagtcc gttcagtttc ggtcatttcc gataaattcc tgttgctttt
    16741 catttctgga ttcccacttt
//
LOCUS       DAXHSF010000026        16643 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.026, whole genome shotgun sequence.
ACCESSION   DAXHSF010000026 DAXHSF010000000
VERSION     DAXHSF010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16643
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.026"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..287
                     /locus_tag="ACFM8S_000686"
     CDS             <1..287
                     /locus_tag="ACFM8S_000686"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313793.1"
                     /translation="IRCFISLLRESRGGADTTLVLVSVISSFFSSREYIDSRRQVLDT
                     AFSYKSDGRMVLFKSIKQESISFLSEDLKKDWMLHKVKTGKKMDMVQMKC"
     gene            complement(802..1164)
                     /locus_tag="ACFM8S_000687"
     CDS             complement(802..1164)
                     /locus_tag="ACFM8S_000687"
                     /inference="COORDINATES: protein motif:HMM:NF018933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFC6313794.1"
                     /translation="MKAARRQLYYTFANRIAEQSRIICIKKLNLLNLQCIQIEVNKLH
                     QKIVAPSPLLHTLKEGAAKNGVHIEEIKANATSQSCYSCGRLNKRIGVAATRTNMVCE
                     GFGRHYDTDENASSNIRK"
     gene            1584..2948
                     /locus_tag="ACFM8S_000688"
     CDS             1584..2948
                     /locus_tag="ACFM8S_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFC6313795.1"
                     /translation="MQHLTERMTQIAKLLNSSPNNPDIDIPDFLTEIKDYSEFSVTDE
                     NGTYLHWDKFRRIHTEDTRMKWRAVKESRKKIQKPIDFPFEHQFWFCIPDSLQARLHL
                     IDKSCSSSIGTSSLGGFGRSEQNRFLLKSLIMEEAITSAQLEGAATTRKVAKDMLKSQ
                     RKPKTKDEIMIVNNYHLMKKAVELKNTPLSVEMILDLHRIATSNAIENKAEPGQFRQD
                     DEIFIADINGNSLYQPPPHGQVHTLMEEVCAFANNTYDGVENPFIHPVVQAIILHFLI
                     GYIHPFGDGNGRTARALFYWFMLKNGYWLFEYISISRLLKNAPAQYAKSYLYAETDDL
                     DLTYFIYYQCDIIKRAVADLEHYISDKQKHQQEFKAAIAQYTEKIGKLNQRQIGILQK
                     AVEESGKIFTAQEIANQYGISLNTARSDLSKLGEYRFLVPFKSGNALEYVAPQDLLER
                     LEKK"
     gene            3309..3815
                     /locus_tag="ACFM8S_000689"
     CDS             3309..3815
                     /locus_tag="ACFM8S_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313796.1"
                     /translation="METTFALLNFKESLCYIYPPTEPDRYVSSIVALNVHSPNGTGDW
                     HSAKALSDRAYPEKFYIYGENQERNTNHLLGDNGIIDGTDRLNKMGYFPENIPVWLAD
                     HPRACVDYLYTAVLQTGSIGRVILDDWFPSDEDKQSVYDLLNQIEPHLNTQEWENLQL
                     WKRKNPIM"
     gene            3942..5231
                     /locus_tag="ACFM8S_000690"
     CDS             3942..5231
                     /locus_tag="ACFM8S_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HFC6313797.1"
                     /translation="MMKSILTVSGNRMRKPRITYLDVWANDERIGTLEKGAMYRFTYD
                     NPNSSLLGLHYQDRSKVYISNNMPHIFAQYFPEGFLAAHITSKYAFHDAPFEDNEMLR
                     LAILGREALGRIHVRCNDPLFNEWIDGLEMKNPRILTERDLLGINARQVFQQYMAEIF
                     HHGRFVSVSGIQQKMSLDAISRNTKQTASYIAKGFDASEYPCLAANEFLCMQTIKQAG
                     IAVAQTSLSEDSSVLLVRRFDVSEQGYFLGMEDFTSLRQYSAEDKYKGSYAAIAQIIR
                     QISGRPDEDLIHFFNQLAASCILKNGDAHLKNFSVLYHDEYDVRLAPAYDVLDTSIYR
                     VGTQGIFDAYDDTLALNLTNHGKKTYPSKNTLLDFAEKYCDLGREDASFMIDTIVQAK
                     EQVLVKYSDVLRENEWLAQKWHFIPDENEEGLPFTFR"
     gene            complement(5568..7355)
                     /locus_tag="ACFM8S_000691"
     CDS             complement(5568..7355)
                     /locus_tag="ACFM8S_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFC6313798.1"
                     /translation="MKLPLSYLPNIRFLSWCCLLAGIIAPATLLASPNPAEIRMQQDI
                     QQRQREEQLRQTMQPESDVRLHQKNTGETVNQLMGDDSSQPCFAINEVVLEGEHHDRF
                     QFALKRALRETGFQAGKCLHAGNINQIMSLAQNALIGRGYTTTRILAAPQDLNSGKLQ
                     LTLIPSYLRSIRIDRSNDDQTHAGRIAAFQNKFPTRSNDLLNLRDLEQGLENLKRLPT
                     AEADLQIVPVEGEPNQSDVVVQWRQRLLPYRVSVGMDNSGSEATGKYQGNITFSADNP
                     FGLSDMFYVNYGRSIGGTPDEESFDGHRKEGGSNNYAVHYSAPFGKWTWAFNHNGYRY
                     HQAVSGLSEVYDYNGKSYNTDFGFNRLLYRDAKRKTYLGVKLWTRETKSYIDDAELTV
                     QRRKTAGWLAELSHKEYIGRSTADFKLKYKRGTGMKDALRAPEEAFGEGTSRMKIWTA
                     SADVNTPFQIGKQLFAYDTSVHAQWNKTPLTSQDKLAIGGHHTVRGFDGEMSLSAERG
                     WYWRNDLSWQFKPGHQLYLGADVGHVSGQSAKWLLGQTLAGTAIGIRGQIKLGGNLHY
                     DIFTGRALKKPEFFQSRKWASGFQVGYTF"
     gene            complement(7410..7868)
                     /locus_tag="ACFM8S_000692"
     CDS             complement(7410..7868)
                     /locus_tag="ACFM8S_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HFC6313799.1"
                     /translation="MPITPPLNIISPKLYLNEQWNESEALGAITWLWYQSPTHRQVPI
                     VEMMTYILPVLKNGQFALFCKGTQPIGYISWAYFDEVAQAHYLESDRHLRDNSDWNCG
                     DNIWLIQWFAPLGHSHQMRSAVRQLFPSTTVRALYHKGSDKGLRILTFKT"
     gene            complement(8032..8346)
                     /locus_tag="ACFM8S_000693"
     CDS             complement(8032..8346)
                     /locus_tag="ACFM8S_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313800.1"
                     /translation="MKKILSNLMLISFCSTMLTACPFWGSCANWNCSELNPRWVKSVH
                     NCRSNNYKVVLSAIGSKFNYQSTSQVQDSDIGQHISGQCTYYDGRYDVRKDKQYGHLF
                     VQ"
     gene            complement(8327..15859)
                     /locus_tag="ACFM8S_000694"
     CDS             complement(8327..15859)
                     /locus_tag="ACFM8S_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HFC6313801.1"
                     /translation="MNKTLYRVIFNRKRGAVVAVAETTKREGKSCADSDSGSAHVKSV
                     PFGTTHAPVCRSNIFSFSLLGFSLCLAVGTANIAFADGIIADKAAPKTQQATILQTGN
                     GIPQVNIQTPTSAGVSVNQYTQFDVGNRGAILNNSRSNTQTQLGGWIQGNLWLARGEA
                     RVVVNQINSSHSSQMNGYIEVGGRRAEVVIANPAGIAVNGGGFINASRATLTTGQPQY
                     QTGALTGFQVRSGHIAITGQGLDARDTDFTQILSHAVKIDGPVWGKDVRVSAGKNDVS
                     ADGSIQSSHSPATNINSDNSLYAIDTGALGGMYAGKITLISTDRDASVRNQGQWFASA
                     GNVAIDANGRLVNSGTMAAANVQDMNNTAEHKVNIRSQAFENSGTAVSQQGTQIHSQS
                     IQNTGTLLSSGEILIHNSGSLKNETSGTIEAARLAIDTDTLNNQGKLSQTGSQKLHID
                     AQGKMDNRGRMGLQDTAPTASNGSSNQTGNSYNASSHSSTTTPTTATGTGTATVSISN
                     ITAPTFADGIIRTHGALDNSGSIIANGQTDVSAQQGLNNAGQIDIHQLNAKGSAFDNH
                     NGTIISDAVHIQAGSLNNQNGNITTRQQLEIETDQLDNAHGKLLSAEIADLAVSGSLN
                     NQNGEIATNQQLIIHDGQQSTAVIDNTNGTIQSDRDVAIQAKSLSNNGTLAADNKLDI
                     ALQDDFYVERNIVAGNELSLSTRGSLKNSHTLQAGKRIRIKANNLDNAAQSNIQSGGT
                     TDIGTQHNLTNRGLIDGQQTKIQAGQVNNIGTGRIYGDNIAIAATRLDNQDENGTGAA
                     IAARENLNLGIGQLNNRENSLIYSGNDMAVGGALDTNSQATGKAQSIRNASATIEAAG
                     KMRLGVEKLHNTNEHLKTQLVETGREHIVDYEAFGRHELLREGTQHELGWSVYNDESD
                     HLRTPDGAAHENWHKYDYEKVTQKTQVTQTAPAKIISGNDLTIDGKEVFNTDSQIIAG
                     GDLLVQTEKDGLHNEQTFGEKKVFSENGKLHSYWREKHKGRDSTGHSEQNYTLPEEIT
                     RNISLGSFAYESHRKALSHHAPSQGTELPQSNGISLPYTSNSFTPLPSSSLYIINPVN
                     KGYLVETDPRFANYRQWLGSDYMLDSLKLDPNNLHKRLGDGYYEQRLINEQIAELTGH
                     RRLDGYQNDEEQFKALMDNGATAARSMNLSVGIALSAEQVAQLTSDIVWLVQKEVKLP
                     DGGTQTVLVPQVYVRVKNGDIDGKGALLSGSNTQINVSGSLKNSGTIAGRNALIINTD
                     TLDNIGGRIHAQKSAVTATQDINNIGGILSVEQTLLLNAGNNINSQSTAASSQNTQGS
                     STYLDRMAGIYITGKEKGVLAAQAGKDINIIAGQISNQSEQGQTRLQAGRDINLDTVQ
                     TSKHQATHFDADNHVIRGSTNEVGSSIQTKGDVTLLSGNNLNAKAAEVSSANGTLAVS
                     AKNDINISAGINTTHVDDASKHTGRSGGGNKLIITDKAQSHNETAQSSTFEGKQVVLQ
                     AGNDANILGSNVISDNGTRIQAGNHVRIGTTQTQSQSETYHQTQKSGLMSAGIGFTIG
                     SKTNTQENQSQSNEHTGSTVGSLKGDTTIVAGKHYEQIGSTVSSPEGNNIIHAQSIDI
                     QAAHNKLNSNTTQTYEQKGLTVAFSSPVTDLAQQAIAVAQSSKQVGQSKNGRVNAMAA
                     ANAGWQAYQTGKSAQNLANGTTNAKQVSISITYGEQQNRQTTQVQASQAQASQIQAGG
                     KTTLIATGAAEQSNINIAGSDIAGKAGTILIADNDITLQSAEQSNTERSQNKSTGWNA
                     GAAVSFGQGGWSLGVTAGGNVGKGYGNGDSVTHRHSHIGDKGSQTLIQSGGDTTIKGA
                     QVRGKGVQVNAKNLSIQSVQDSETYQSKQQNASAQVTVGYGFSASGDYSQSKIRANHA
                     SVTEQSGIYAGEDGYQIKVGNHTDLKGGIITSTPSAEDKGKNRFQTATLTHSDIKNHS
                     QYKGESFGLGASASISGKTLGQGAQNKPQNKHLTSVADKNGASSSVGYGSDSDSQSSI
                     TKSGINTQNIQITDEAAQIRLTGKTAAQTKADIGTNVTTDTAERHSGSLKNTFNREAV
                     QNELDLQREVTQEFDKTRQGVKQELYAVVDSKRAQATQERLKNGGYDNDKSRALNKEV
                     NELDEKIRWLDAGLGLVWGAGSSDMAWSMFATTQADRAVRSATAPKEMWFHKKIIDEK
                     TGKVSFDTRQIWSLNDLSKEELASIQDTNGKVITVSNPGIFNNREDSLSNAAKQNRNS
                     TNGSGVIAVMNPPTGKYKSDSNNKIKDFLWLGSSLVSELMYVGYDQLNNKVFQGYLPK
                     TNSEKLNQDIYREVQKMGNGWSVDTSNHSRGGITASVSLKDWVNNQKQNGIAPIRKAR
                     FYGTATNVQNDYADVLQKNGYTYTGADGKTYNSGSYSIVHDKDFVGNKWIPFLLGNNE
                     TTKGACKGFCYSHSSYFAEVPEQYKRDKNGNFVTDNEGNKIETKDWDSYTKIWGIPKK
                     GTDGKDINHAIPKLVNPNKPNGEKYEENPF"
     gene            16015..16167
                     /locus_tag="ACFM8S_000695"
                     /pseudo
     CDS             16015..16167
                     /locus_tag="ACFM8S_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4248 a         3552 c         3651 g         5192 t
ORIGIN      
        1 cgatacgctg tttcatctct ttgcttaggg agagtagggg gggggcggat acgaccttag
       61 ttttggtatc cgtaatatca tcattttttt cgtcaaggga gtatatcgac tctagaagac
      121 aggtattaga tactgccttt tcttacaaga gtgatggtag gatggttctc ttcaagtcaa
      181 tcaaacagga aagtatttct tttctatctg aagatttgaa aaaggactgg atgttacaca
      241 aggttaaaac tgggaaaaag atggatatgg ttcagatgaa atgctgagcg taccctgtgt
      301 ctatttggaa atgatgtcgc ggaaaacggg agtcccctac tccagtattc tttaaagtct
      361 aagtagaaga cttcttcgtc ggtctttttt tgttgtttgg tttgcatgga gtaaaactgt
      421 acaactgttg aagcataaaa ccagcaagaa ggtaaaaaag aaagaagcag ttctttggat
      481 tttagatatt accgcaatta gtttcctttc ctaaaatttg tttaaattat ttgcaatatt
      541 aatataaacg agatattaat gatgagaaat caaaaaggca tactaaatac aatttgtaca
      601 aaataattgc agtatttaaa aatgttgatt cgtatatgaa aagttaaaaa tgccaaaatg
      661 tacagttgct aaactgtaaa actgccaaag caacaaaaca taaaaaggaa tgcagggatg
      721 caatcactac atctttttat tccgtaagca tttatgactt tacggtcaac tgctactcta
      781 tgtttccagc ttttcagctc cctattttcg aatattggac gaggcatttt catcagtgtc
      841 gtaatgccgg ccgaaaccct cacaaaccat attggttctt gtggcagcaa cacctatccg
      901 tttgttcaag cggccacaag agtaacatga ttggctggtg gcattggctt taatctcttc
      961 gatatgaact ccatttttag ctgcaccttc tttcagcgta tgcaacagtg gggatggggc
     1021 aactatcttc tggtggagtt tgttgacttc aatctgtatg cactgtaggt tgagcagatt
     1081 cagttttttg atacagatta tccggctttg ttcggcaatt ctgttggcga acgtatagta
     1141 gagctgtcgt cttgccgctt tcaatttgcg gtaggtattt taccattcca tgcgtagccg
     1201 ctcggtacgt ttgagccaaa tggtatcttc gccatttgta ccaattggtt tttacattcg
     1261 gctgtgtttt agtaatttat taatttcaat tatttgcaag ggaaaagaca attgtttccg
     1321 gttaggaata aacctatcct attgaatata ttgaagccaa gtacgtctat caacactata
     1381 ttaaaacaca gcctttttta atatagtaga cacaatcttt ccctatttat gaaggtgatc
     1441 gcttctttca gattcgtatt ttaatacttt ctatttctag aaaatttgac taaaataact
     1501 caattataaa aaattgcgcg attttggtat ttatcatgaa aatttccaga cctccggaat
     1561 ttaccctgtt gcaacaggaa tatatgcagc atctcactga aagaatgacg caaattgcca
     1621 agctgctgaa ttcttcccca aacaatcctg atatagacat tcccgatttt cttactgaaa
     1681 tcaaagatta ttcagaattt tccgtgacag atgaaaatgg aacctacctg cattgggaca
     1741 aattccgccg gattcacacg gaagatacgc ggatgaaatg gcgcgccgtt aaggaaagcc
     1801 gcaaaaaaat ccaaaaacca attgatttcc cgtttgaaca tcagttttgg ttctgcattc
     1861 ccgactcttt gcaggcacgg cttcatttga ttgacaaaag ctgcagcagt tctatcggca
     1921 cgtctagctt gggtggtttc ggcagaagcg agcaaaacag attcttgctc aagtctctga
     1981 ttatggaaga agcgattaca tccgcccaac tggaaggtgc ggctaccacg cgtaaagtgg
     2041 ccaaggatat gctcaaatcg cagcgtaaac ccaaaacaaa agacgaaatc atgatagtga
     2101 acaactatca cttgatgaaa aaagcggtag aattgaaaaa tacgccgtta agtgttgaaa
     2161 tgattttgga tttgcaccgc attgctacca gcaacgctat tgaaaacaag gccgagcccg
     2221 gacaattcag gcaggatgac gaaatcttta tcgccgatat caatggtaac agcctgtatc
     2281 aaccaccgcc gcacggacag gttcatacgc tgatggaaga ggtgtgtgcg tttgccaata
     2341 atacctatga cggcgtggaa aatccgttta tccatccggt tgtccaagct attatcctgc
     2401 atttcctcat cggctacata cacccatttg gtgatggcaa cgggcggaca gcgcgggctt
     2461 tgttctattg gtttatgctc aaaaacggct actggctatt tgaatacata tccatcagcc
     2521 gtcttctgaa aaacgctcct gcccaatacg ccaaatccta tttgtatgcg gaaactgacg
     2581 atttagattt aacctatttc atctattacc aatgcgatat tatcaagcgg gcggttgctg
     2641 atttggagca ctacatttct gacaaacaaa aacaccaaca ggaattcaaa gcagcgattg
     2701 cccaatatac tgaaaagata ggaaagttga accaacggca aattggtatc ctgcaaaaag
     2761 cagtggaaga aagcggaaaa atctttactg cacaagaaat tgccaaccaa tacggcatct
     2821 ccctgaatac tgcccgtagc gatttgagca aactgggaga atatagattc ctagtgccgt
     2881 tcaaatcagg aaatgcttta gagtatgttg ctcctcagga tttattggaa aggttagaaa
     2941 aaaaatagtt tgctagccta aaatgcagct ttaaccgagt caaaatcaat acagtccgca
     3001 ccttcaaaaa gaagctgcgg actgcttgct ttttgctcta caaatgatct ttgtagctga
     3061 tttaaccaag attgtagcaa ttttgctttc cgagcaagca gggttagaaa attcgatact
     3121 tttaattatt ggctgtgttt taatatgatg ttgataggcg tgtttggctt taaggtattc
     3181 aacaggatag gtttattcct aaccggaaaa taattgtctt ttcccttgca agtaattgaa
     3241 atcaacagat tactaaaaca cagccttaca ttattggggt gactatcctg taaaatatgt
     3301 cctaaaacgt ggaaaccact tttgctctgc taaattttaa ggaatcttta tgttacatat
     3361 accccccaac ggaacctgat cgatatgtta gcagtattgt agccttaaac gtgcatagtc
     3421 ctaacggtac aggcgactgg catagtgcaa aggcattgag tgatcgggct taccctgaaa
     3481 aattttatat ttacggtgaa aatcaagagc gaaataccaa tcatctatta ggtgacaatg
     3541 gcattattga tgggacggat cgactgaaca aaatgggtta tttccctgaa aacatcccag
     3601 tttggctcgc agatcacccc cgtgcttgcg tggattatct ttacacagca gtgttacaaa
     3661 ctggctcaat cggtcgggtg attttagatg attggtttcc aagcgatgaa gacaagcaat
     3721 cagtttatga cttacttaac caaatcgaac cgcacttgaa tactcaagaa tgggagaatt
     3781 tacagctatg gaaacgcaaa aacccaataa tgtaatgcta accgaacgag ataaaaggca
     3841 cgagaagcgg tattactgaa tttcatccgc aatacgccat gtacaattaa atcagcagat
     3901 gtgtctaaac gtaccgctgt tcccgtttga aagaaatttt catgatgaag tctatcctca
     3961 ccgtatccgg aaatcgtatg cgtaaaccca gaatcaccta tttggatgtt tgggcaaacg
     4021 atgaaagaat cggtactttg gaaaaggggg ccatgtatcg gttcacatac gacaatccca
     4081 attcttcgtt gctgggcctg cattatcaag acagaagcaa ggtatatatc agcaacaaca
     4141 tgccacatat ctttgcacag tattttccgg aaggcttttt ggccgcacac atcacaagca
     4201 aatatgcttt tcatgatgcg ccttttgaag acaatgagat gctgcgcttg gcaattctgg
     4261 gcagagaggc tttgggtcgg atacatgtgc gctgtaatga cccgcttttt aatgaatgga
     4321 ttgacgggtt ggagatgaaa aatccaagaa tattgactga acgggatttg ctgggcataa
     4381 atgcccgaca ggtttttcag caatatatgg cagaaatctt ccatcacggc cgtttcgtca
     4441 gtgtatccgg gatacagcag aagatgtcct tagatgccat cagcagaaat accaagcaaa
     4501 ctgcctcata tattgccaaa ggttttgatg catccgaata tccttgcttg gctgccaatg
     4561 aatttttatg catgcagacc atcaaacaag ccggcattgc cgttgcacag accagcctgt
     4621 cggaagattc atcagtctta ttggtacgtc ggtttgatgt cagtgaacag gggtatttct
     4681 taggcatgga agactttacc agtctgcgtc agtattcagc agaagataag tataaaggca
     4741 gttatgcggc tattgcacag attatccgac agatatccgg cagacccgat gaagatttaa
     4801 tccatttctt taaccaactt gccgccagtt gcatattgaa aaacggcgat gcacacctca
     4861 aaaatttttc agtactctat catgacgaat acgatgtacg tcttgcacct gcctatgatg
     4921 tattggatac atcaatatac agggttggaa cacaaggaat ttttgatgct tatgacgata
     4981 cgctggcatt aaacctgact aaccacggta agaaaacata tccttccaag aatacattgt
     5041 tggattttgc tgagaaatat tgcgatttgg gaagagaaga tgcatccttt atgatagata
     5101 caatcgttca agctaaagaa caggttcttg ttaaatactc ggatgtattg cgtgagaatg
     5161 aatggttggc gcagaagtgg cattttatcc cggatgaaaa tgaagaaggt ctaccgttta
     5221 cattccggta gctgccgggt ttgaaccggc tgtcagagat ggccggtcga ctttcaccct
     5281 gaaaatcact tcatcttatg gtgtttgaaa ccgagaaatt agaagaatcg tattcggtag
     5341 gagatatact aggaagattg gaaaactggt aaatcattct attgattgag ttggcggcct
     5401 atatgtttaa tgtaggcaaa cgagaaggaa tcatgtattt catgattcct tcttaaattc
     5461 ctgtgtcaat ctaatatcaa aacacagcca tctcttacca taatcatgat aggtgtttta
     5521 ttatgaaaag ctatatctat aattaccgtt gatttgacta tgccgtttta aaacgtatag
     5581 cctacctgaa aaccgcttgc ccatttcctt gattggaaaa attcgggctt tttcaatgcg
     5641 cggccggtaa atatatcgta atgcaggttg ccgccaagct ttatctgccc gcgtatccca
     5701 attgctgtgc cggctagagt ttggcccaat aaccatttgg cggattgtcc tgaaacatgt
     5761 cctacatcag ccccaagata aagctgatgg cctggtttaa attgccagct caaatcgttg
     5821 cgccaatacc atccccgctc ggcagacaaa ctcatttcac cgtcgaagcc acgtacggtg
     5881 tggtgtccgc cgatagccag tttgtcttgc gatgttagcg gggttttgtt ccattgtgca
     5941 tgaacggatg tgtcataggc aaatagctgt ttaccgattt gaaaaggagt atttacatca
     6001 gccgatgccg tccaaatttt catacgtgac gtgccttctc caaaggcttc ttcaggcgcg
     6061 cgcagagcat ctttcatgcc ggtgccgcgt ttatatttca acttaaaatc tgccgtactg
     6121 cggccgatat attctttgtg ggaaagttct gccaaccaac ccgcagtttt acgccgttgt
     6181 acagtcagtt cggcatcatc aatgtaactt tttgtttccc tcgtccacag ttttacacca
     6241 agataggttt tgcgtttggc atcacgatac aacaggcggt tgaagccgaa atcagtattg
     6301 taactttttc cattatagtc atagacttcc gataatccgg aaaccgcctg atggtaacgg
     6361 tagccattgt gattgaatgc ccatgtccat ttaccgaaag gggctgaata atgtacggcg
     6421 taattgtttg atccgccttc tttgcgatgg ccgtcaaaac tttcctcgtc gggcgtaccg
     6481 ccaattgaac gtccataatt tacatagaac atatcactca gtccaaaagg attgtcggca
     6541 gagaaagtga tatttccttg gtattttcct gtcgcctcac tacccgaatt atccatcccc
     6601 acactcacac ggtagggcag cagacgttgc cgccattgca ccacgacatc actttggttt
     6661 ggttctccct ctacgggaac gatttggaga tcggcttccg cagtcgggag acgtttgaga
     6721 ttttccagtc cttgttccaa atcacgcaga ttcaacagat cgttcgagcg ggtgggaaat
     6781 ttgttctgga atgctgcaat acgtcctgca tgggtttgat catcgttaga ccgatcgatt
     6841 cgtatggagc gcagatagct cggtatcagg gttaattgaa gcttgccact attcaaatcc
     6901 tgtggcgcag ccaagatacg ggtcgtggta tatcccctgc cgatcaaagc attttgtgct
     6961 aaggacatga tttgattaat gttgcccgca tgcagacact tgccagcctg aaaacccgtt
     7021 tcgcgcaagg cacgttttag ggcaaactga aaccgatcat ggtgttcacc ttccaacacc
     7081 acttcgttaa tggcaaaaca cggttggctg ctgtcatcgc ccatcaactg attaaccgtt
     7141 tcccccgtgt ttttttgatg caaacgcaca tcgctttcag gctgcatggt ttggcgcaac
     7201 tgctcttcgc gttggcgttg ctgaatatct tgctgcatac ggatttcggc agggttgggg
     7261 gaagccaaca aagtagcagg agcgatgata cctgccaata agcagcacca agacaaaaag
     7321 cgaatattag gcaaatagga taaaggaagt ttcatggcat ggtcgcaaaa tcaataaaat
     7381 catcaatggg cattcaatac acgattgcat caagttttaa aagttaaaat tctcaaaccc
     7441 ttatcgctcc ctttatgata caaggcgcgt actgtcgtac taggaaataa ctggcgcaca
     7501 gctgagcgca tttgatgact gtgtcccaat ggcgcaaacc attgaatcag ccaaatattg
     7561 tcgccacagt tccaatcgct gttgtcgcgc aaatggcggt cagattctaa ataatgcgcc
     7621 tgcgccactt catcaaaata agcccatgag atataaccga ttggttgggt acccttgcaa
     7681 aacaaagcga actgcccgtt ttttaacaca ggcaatatat acgtcatcat ctccacaata
     7741 ggtacttggc gatgcgtagg cgactgatac catagccaag tgatggcacc gagtgcttcg
     7801 ctttcgttcc attgttcatt gaggtagagt ttaggggaga tgatgtttaa gggtggagtg
     7861 atgggcatat tttaagttag ggttggtgtt ggttaaaaca aaaaatggtt tcagcctgaa
     7921 atgaaatatc tcacgttaaa acatagcttt agcatacgag actgaattgt ggggcaataa
     7981 gcaacgaccc taccattcaa caattctcgt taattgcaca ggttacgctg gttattgcac
     8041 aaacaaatga ccatactgct tatctttgcg aacatcgtat ctgccatcat agtaagtaca
     8101 ttgtccagaa atatgttgcc caatgtcact atcttgaact tggcttgttg attgatagtt
     8161 aaatttactt cctattgccg ataacacaac tttatagtta tttgatcgac aattatgaac
     8221 tgatttcacc cagcgtggat tgagttcgga acaattccag tttgcacaac tgccccaaaa
     8281 agggcaagct gttagcattg ttgagcagaa tgatattagc attaaattag aaaggatttt
     8341 cttcatactt ttctccattt ggcttgtttg gattgactaa tttgggaata gcgtgattta
     8401 tgtcttttcc atcagtacct tttttaggaa ttccccatat ttttgtgtaa ctgtcccagt
     8461 ctttcgtttc aattttattc ccttcattat cagtaacaaa atttccattt ttgtctcgct
     8521 tatactgttc aggcacttcc gcaaaataac tgctatgcga atagcaaaat cccttacaag
     8581 ctccttttgt tgtctcatta tttcctagca agaaaggtat ccatttgttc cccacaaaat
     8641 ctttatcatg cacgattgag taggatccgc tgttataagt tttgccgtct gcacccgtat
     8701 aggtatagcc gtttttctgt aaaacatcgg cgtaatcatt ctgcacattt gtggctgtac
     8761 catagaaacg tgcttttctg attggggcaa tgccattttg tttttgattg tttacccaat
     8821 cttttaagga aacgcttgct gtaattcccc cacgactgtg attactggta tcaaccgacc
     8881 agccgttacc cattttttga acctctcgat aaatatcttg attcagtttt tctgaattgg
     8941 ttttgggtaa atagccttgg aacactttat tatttaattg gtcgtaaccg acatacatca
     9001 gttcagaaac aagacttgaa ccgagccata aaaaatcttt tattttgtta ttagaatcag
     9061 atttatattt ccctgttgga ggattcataa ctgcaataac accactaccg tttgtactat
     9121 tacgattttg ttttgctgcg ttgcttaatg aatcttctcg attattgaaa ataccaggat
     9181 tagacacagt aataactttg ccatttgtgt cttgaatgct tgccagttct tccttgctta
     9241 aatcattcaa tgaccaaatt tgtctggtat caaaggatac tttacctgtt ttttcatcta
     9301 ttatcttctt atggaaccac atttctttag gtgcagttgc agaccttacc gctctatcgg
     9361 cttgggttgt ggcaaacata ctccaagcca tatcagaact ccctgcgccc caaacgagtc
     9421 ctaatcccgc atcaagccaa cgaatttttt catctaattc attaacttcc ttatttaatg
     9481 ctctagattt atcattgtca tatccaccat ttttgagtct ctcttgtgtg gcttgcgctc
     9541 gtttgctatc tactactgca taaagttctt gtttcactcc ttgtctagtt ttatcaaact
     9601 cttgggttac ttccctttgc aaatccagct cgttttgcac cgcttctctg ttgaaggtgt
     9661 tcttcaagct gcccgaatgt cgttcggcgg tgtctgtggt tacgtttgta ccaatatcgg
     9721 ctttggtttg tgccgctgtt ttgcctgtca gcctgatttg tgcggcttcg tcggtgattt
     9781 gaatgttttg ggtgttgatg ccgcttttcg tgatgctgct ttgactgtcg ctgtcgctgc
     9841 cataacccac tgatgaactt gcgccgtttt tatcggctac gcttgtcagg tgtttgtttt
     9901 gaggcttatt ttgtgcgccc tgtcccagtg ttttgccgct tatggacgca cttgcgccca
     9961 atccaaaact ttcgcctttg tattggctgt ggtttttgat gtcgctatgg gtgagggtgg
    10021 ccgtctgaaa gcggttttta cccttgtctt ctgcgcttgg ggtactggtg atgatgccgc
    10081 ctttgaggtc tgtatggttt ccgaccttga tttgatagcc gtcttctccg gcataaatac
    10141 cgctttgctc ggttaccgaa gcatgattgg ctcggatttt gctttggctg taatcgccac
    10201 tggcactgaa gccataaccg acggtcactt gtgcactggc gttttgttgt ttgctttgat
    10261 aggtttcgct atcttgtacg ctttgaatac ttaggttttt ggcattgact tgtacgcctt
    10321 tgccgcgtac ttgcgcgcct ttgatggtag tgtcgccacc gctttggata agggtttggc
    10381 tgcctttgtc gccaatatgg ctatggcggt gggttacgct atcgccattg ccgtagcctt
    10441 tgccgacatt gccgcctgcg gtaacgccta atgaccagcc tccttgtccg aatgatacgg
    10501 cagcacctgc gttccaacct gtcgatttgt tttggctgcg ttcggtattg ctttgctcgg
    10561 ctgattggag tgtgatgtcg ttatcggcaa tcaggattgt gcctgctttg ccggcaatat
    10621 ctgagcctgc gatgttgata ttggattgtt ctgctgcgcc tgtggcgatt aatgtggttt
    10681 taccgcctgc ttgaatttga ctcgcttggg cttggcttgc ttgaacttgg gtggtttgtc
    10741 ggttttgctg ttcgccgtag gttatggaga tgctgacttg tttggcattg gttgtaccat
    10801 tggctaagtt ttgtgcactc ttacctgttt gataggcttg ccagcctgca ttggcagctg
    10861 ccatggcatt gacacgacca tttttgcttt gtccgacttg tttgctgctt tgtgcgacgg
    10921 caatcgcttg ttgtgccaaa tcggtaacgg gcgaactgaa tgccaccgtt aagccttttt
    10981 gttcataggt ttgggtggta ttactgttta atttgttgtg tgccgcttga atgtctatgc
    11041 tttgggcatg gatgatattg ttgccttctg ggctggaaac ggtactgccg atttgttcgt
    11101 agtgtttgcc tgcaacaatg gtggtatcgc ctttcaagct gcctacggta ctgcctgtat
    11161 gttcgttgct ttgggattgg ttttcttgtg tgtttgtctt gctgccaata gtgaagccga
    11221 tacctgcact catcaatcct gatttctggg tttgatgata ggtttcgctt tggctttgag
    11281 tttgggttgt accaatgcga acatgattgc ctgcttgaat ccgggtgcca ttatcggaaa
    11341 taacattgct gccaaggata ttggcatcgt ttcctgcctg caatacaact tgcttgcctt
    11401 caaaggtgct gctttgagca gtttcgttat ggctctgggc tttgtcggta ataatcaatt
    11461 tattgccacc accgcttctg cctgtgtgtt tggacgcatc atcaacatgg gtcgtgttga
    11521 tgcctgcact gatgttgatg tcatttttgg cagacacagc gagtgtaccg tttgcgctgc
    11581 tgacttcggc agctttggca ttgaggttat tccctgacaa tagggtaaca tcgccttttg
    11641 tttgaatgct gctgccgact tcgttcgttg aaccgcgaat aacatggtta tcggcatcaa
    11701 aatgggttgc ttgatgtttg ctggtttgta ccgtatctag gttaatgtcg cgccctgctt
    11761 gcagccgggt ttgcccttgc tctgattgat tgctgatttg accggcaatg atgttgatgt
    11821 cttttcctgc ctgcgctgct aaaacacctt tttctttgcc tgtgatataa atacctgcca
    11881 ttcggtctag gtaggtgctg ctgccttgtg tattttgact gctggcggcg gtgctttggc
    11941 tgttgatgtt gttgcctgcg ttgagcaata atgtctgttc gacagaaaga atgccgccaa
    12001 tattattgat gtcttgtgtg gccgtaaccg ctgatttttg cgcatgaata cgcccaccga
    12061 tattgtctag cgtatcggta ttgataataa gcgcattgcg ccctgcaatc gtgcctgagt
    12121 ttttcaggct gcctgaaaca ttgatttgtg tattgctgcc tgacaacaat gcacctttac
    12181 cgtctatgtc gccattctta acgcgtacat aaacctgtgg caccaatacg gtttgtgtgc
    12241 cgccatcagg aagtttaact tctttttgta ccaaccaaac aatatcgctg gtcagttgcg
    12301 ctacttgctc ggcacttaat gcaatgccaa cgctgagatt catggagcgt gccgcagtcg
    12361 cgccattatc cattaaggct ttaaattgtt cttcgtcgtt ttgataaccg tctaaacgac
    12421 gatgccctgt cagctctgcg atctgttcat tgattaaacg ttgctcgtaa taaccatcac
    12481 ccaaacgttt atgtaaattg tttgggtcta gtttgaggct gtccagcata tagtcactac
    12541 ccaaccattg acggtagttg gcaaagcgtg gatcggtttc aacaagatag cctttattga
    12601 caggattgat aatgtataag ctgctgctgg gtaatggggt aaaagaattg gacgtatagg
    12661 gtagcgaaat accgttgctt tgcggcaact cagtgccttg gctgggcgca tgatggctta
    12721 atgctttgcg atgcgattca taggcaaatg aacccagtga aatgttgcgt gtgatttcct
    12781 ccggcaaagt gtaattttgt tcgctatgtc ccgttgagtc tcgtccttta tgtttctcac
    12841 gccaatagct gtgtaatttg ccattttcac tgaatacttt cttttcgcca aaggtttgct
    12901 cgttatgcaa accgtctttt tctgtttgca caagcaaatc gccgccggcg atgatttggc
    12961 tatcggtatt aaatacttct ttaccatcaa tggttaaatc attacctgaa atgattttgg
    13021 ctggcgcagt ttgggtaact tgggtttttt gggtgacttt ttcataatcg tatttatgcc
    13081 aattttcatg cgccgctcca tcaggggtgc gtaagtggtc tgattcatcg ttatagacag
    13141 accagcctaa ttcatgttgc gtgccttccc gcaataattc gtgtcgtcca aatgcttcgt
    13201 aatcaacaat atgctcgcgc cctgtttcta ccaactgcgt tttcaaatgc tcattggtat
    13261 tgtgcagctt ttctacacct aaacgcattt tgccggcggc ttcaatggtt gcgctggcat
    13321 tgcgtatgct ttgggctttg cctgtggctt ggctattggt atctaatgcg ccgccaaccg
    13381 ccatatcgtt accgctgtaa atcagactgt tttcacggtt gtttaattgt ccgatgccta
    13441 aattcaggtt ttcgcgtgcc gcaatggcgg cacctgtacc gttttcatct tgattgtcta
    13501 agcgggtagc cgcaatagcg atattgtcac cataaatccg acctgtaccg atattattca
    13561 cttgcccggc ttggattttg gtttgttgtc cgtcaatcaa gcctctattg gttaaattgt
    13621 gctgcgtgcc aatgtctgtc gtaccgccgg attgaatgtt gctttgtgct gcattatcaa
    13681 ggttatttgc tttaatccga atgcgttttc ctgcttgcaa ggtatgtgaa tttttcaggc
    13741 tgcctcgtgt actgagcgac aattcattgc ccgccacgat attgcgttct acataaaaat
    13801 catcttgtaa cgcaatatcc agtttattat cagcggcaag tgtgccgttg ttggataacg
    13861 attttgcctg aatagcaaca tcacggtctg attgtatcgt gccattcgta ttatcaatga
    13921 cagcggtaga ttgctgacca tcgtgaataa tcagttgttg attggtcgct atttcgccat
    13981 tttgattgtt caggctgcct gaaacggcta aatccgctat ttctgctgat aataacttgc
    14041 catgagcgtt atccagttga tcggtttcaa tctctaactg ttggcgtgtt gtgatgttgc
    14101 cgttttgatt attcaggctg ccggcttgaa tgtggaccgc atcactgata attgttccat
    14161 tgtgattgtc aaacgccgaa ccttttgcat ttaactgatg aatgtctatt tgtcctgcat
    14221 tatttaaacc ttgttgcgca ctaacatctg tttgaccatt ggcaataata ctgcctgaat
    14281 tatccagtgc accatgagta cgaattatcc catcagcaaa ggtaggcgca gttatgtttg
    14341 atatagaaac ggttgcagtc cccgtacctg ttgccgttgt tggtgtggta gtggatgaat
    14401 gggaagatgc attgtaacta ttgccggttt gattgcttga accatttgac gcggttggtg
    14461 cggtatcttg taaacccatg cggccacggt tatccatttt gccttgtgca tcaatatgga
    14521 gtttttgtga acctgtttga gagagtttgc cttgattatt aagtgtgtcg gtatcaatag
    14581 ccaaacgagc ggcttcaatg gtgcctgatg tttcattttt caggctgccc gaattgtgaa
    14641 tcaatatttc gcctgaggac aataatgtgc cagtgttttg aatcgactga ctgtgaattt
    14701 gagtgccttg ttgcgatacc gccgtaccgc tgttttcaaa ggcttgactg cggatattga
    14761 ctttgtgttc cgctgtatta ttcatatctt gcacattggc ggcagccatc gtgccactat
    14821 tgaccaaacg gccatttgca tcaatcgcca cattgccggc tgaggcaaac cattgcccct
    14881 gattgcgcac ggatgcatca cgatcagtgc tgatcagggt gatcttaccg gcatacatac
    14941 cgcccaatgc ccctgtatcg atggcataca gtgagttgtc ggaatttata ttcgtagcag
    15001 gggagtgtga agactggata gagccatcag ctgagacatc atttttccct gccgacacac
    15061 gcacatcttt accccacaca ggcccatcaa tcttaaccgc atggctcaat atctgcgtga
    15121 aatcggtatc acgtgcatcc aaaccttgtc ccgtgatcgc aatatgaccc gaacgaacct
    15181 gaaagcctgt taatgcgcct gtttgatatt gcggttggcc tgtcgtcaaa gtggcacggg
    15241 aagcattgat aaaaccacca ccattgactg caatccctgc cggattggca ataacgactt
    15301 ctgcacgtcg tccgcccact tcaatatagc cattcatttg tgaagaatgg ctgctgttga
    15361 tttggtttac aaccacacgt gcttcgcccc ttgccaacca aagattgcct tgaatccaac
    15421 cgcctagctg tgtttgggtg ttgctgcggc tgttgtttaa aatcgccccg cgattaccca
    15481 catcaaactg tgtatattga ttaacagaaa cccctgccga agtaggggtt tgaatattga
    15541 cttgcggtat gccgttacct gtttgcagaa tcgtggcttg ttgggtttta ggagcagctt
    15601 tatcagcaat aatgccatca gcaaaagcaa tattggccgt acctacagcc aaacataaag
    15661 aaaagcccaa taaagaaaaa gaaaagatat ttgaacgaca aacaggtgca tgagtagtac
    15721 caaaaggaac agatttcaca tgagcgctgc ctgaatcact atcggcacag cttttacctt
    15781 cgcgcttggt agtttcagca acggctacca cagccccacg tttgcggttg aaaattacac
    15841 gatagagagt tttattcatg atttcagtta tttgattttt atagagttat tagaaaaaat
    15901 tggatagtct gaccattcta gatcaaggat tttggcgagt caattaccgc cattttactg
    15961 ccatttgttt attaaattag ggactttact agataacggt taaaaatccc attcgaacga
    16021 aatggcaagg tttataccgt cgttgtccct aatgcgcaat cagcaacatt attgccgatt
    16081 atccgagaga aagttaagtc tgatggcatt gtgtatacgg atacctttcg tagttatgat
    16141 gtacttgatg tcagtgaatt tagccattta cgcaagtttt ccagtatttg actggcaatt
    16201 taaaacaatc ggaagttgtc ccatttccag tatttgactg gcaatttaaa acaatcggaa
    16261 gttgtcccat ttccagtatt tgactggcaa tttaaaacag tcggattttg tcccatttgt
    16321 tggccaagtc tttacttgct tggccgtttg aatttaaaaa gcagtctttc tactttccga
    16381 ccttttttct gttgtaaggt ctatgatcca atagcattcc caaagagcat tttggacggt
    16441 atttaaagga atgtgaatag cgttttaaca acagtgaaat agaatctaaa attcagattt
    16501 gaagacaatt agttaatggg aatttggtct ggttatctag tacagcctct aaaattttag
    16561 tgaccgaaca aactcgaaaa atccacatcg ccgatgcgtt ccagccggtc ggcaaagctg
    16621 tcaagattca cagattcagc gtt
//
LOCUS       DAXHSF010000027        16522 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.027, whole genome shotgun sequence.
ACCESSION   DAXHSF010000027 DAXHSF010000000
VERSION     DAXHSF010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16522
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.027"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(86..2191)
                     /gene="fusA"
                     /locus_tag="ACFM8S_000696"
     CDS             complement(86..2191)
                     /gene="fusA"
                     /locus_tag="ACFM8S_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFC6313802.1"
                     /translation="MARKTPISLYRNIGISAHIDAGKTTTTERILFYTGLTHKLGEVH
                     DGAATTDYMEQEQERGITITSAAVTSYWSGMAKQFPEHRFNIIDTPGHVDFTVEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRLAFVNKMDRQGANFFRVVEQMKT
                     RLRANPVPIVIPVGAEDNFSGVVDLLKMKSIIWNEVDKGTTFTYGDIPAELVETAEEW
                     RQNMIEAAAEASEELMDKYLGGDELTEEEIVGALRQRTLAGEIQPMLCGSAFKNKGVQ
                     RMLDAVVELLPAPTDIPPVQGVNPNTEEADSRQASDEEKFSALAFKMLNDKYVGQLTF
                     IRVYSGVVKSGDTVLNSVKGTRERIGRLVQMTAADRTEIEEVRAGDIAAAIGLKDVTT
                     GETLCAESAPIILERMEFPEPVIHIAVEPKTKADQEKMGIALNRLAKEDPSFRVRTDE
                     ESGQTIISGMGELHLEIIVDRMKREFGVEANIGAPQVAYRETIRKAVKAEYKHAKQSG
                     GKGQYGHVVIEMEPMEPGGEGYEFIDEIKGGVIPREFIPSVDKGIRDTLPNGIVAGYP
                     VVDVRIRLVFGSYHDVDSSQLAFELAASQAFKEGMRQASPALLEPIMAVEVETPEEYM
                     GDVMGDLNRRRGVVLGMDDDGIGGKKVRAEVPLAEMFGYSTDLRSATQGRATYSMEFK
                     KYSEAPAHIAAAVTEARKG"
     gene            complement(2210..2680)
                     /gene="rpsG"
                     /locus_tag="ACFM8S_000697"
     CDS             complement(2210..2680)
                     /gene="rpsG"
                     /locus_tag="ACFM8S_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFC6313803.1"
                     /translation="MPRRREVPKRDVLPDPKFGSVELTKFMNVLMIDGKKSVAERIVY
                     GALEQIEKKTGKVAIEVFNEAIANAKPIVEVKSRRVGGANYQVPVEVRPSRRLALAMR
                     WVRDAARKRGEKSMDLRLAGELIDASEGRGGALKKREEVHRMAEANKAFSHFRF"
     gene            complement(2798..3169)
                     /gene="rpsL"
                     /locus_tag="ACFM8S_000698"
     CDS             complement(2798..3169)
                     /gene="rpsL"
                     /locus_tag="ACFM8S_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003093744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFC6313804.1"
                     /translation="MPTINQLVRKGRQKPVYVNKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQARSKYGAKRPK"
     gene            complement(3348..3497)
                     /locus_tag="ACFM8S_000699"
     CDS             complement(3348..3497)
                     /locus_tag="ACFM8S_000699"
                     /inference="COORDINATES: protein motif:HMM:NF016462.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC6313805.1"
                     /translation="MSAALWHKKLSWKGFTPQINFRYNKINSNMPAFYSRSGKERFVS
                     IEKTY"
     gene            complement(3494..3751)
                     /locus_tag="ACFM8S_000700"
     CDS             complement(3494..3751)
                     /locus_tag="ACFM8S_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313806.1"
                     /translation="MKPRNLFFAGCLLTSATFAEDIGVPVELINVGNRIAMPSEGESL
                     ALLPFAEDVPPVRDAMPSEVPKSAAGGDVRGDRMRMPINIG"
     gene            complement(4043..8218)
                     /gene="rpoC"
                     /locus_tag="ACFM8S_000701"
     CDS             complement(4043..8218)
                     /gene="rpoC"
                     /locus_tag="ACFM8S_000701"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFC6313807.1"
                     /translation="MNLLNLFNPLQTAGMEEEFDAIKIGIASPETIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKFKGVTCEKCGVEVTLSKVRRE
                     RMGHIELAAPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAFVVTDPGMTPLQRRQ
                     LLTEDDYYNKLDEYGDDFDAKMGAEGIRELLRTLNVAGEIEILRQELESTGSDTKIKK
                     IAKRLKVLEAFHRSGMKLEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELHAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIFHKLEKQGL
                     ASTVKAAKKLVEQEVPEVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPILIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDI
                     VLGLYYMTRDRINAKGEGSLFADVKEVHRAYHTKQVELGTKITVRLREWVKNEAGEFE
                     PVVNRYETTVGRALLSEILPKGLPFEYVNKALKKKEISKLINASFRLCGLRDTVIFAD
                     HLMYTGFGFAAKGGISIAVDDMEIPKEKAALLAEANAEVKEIEDQYRQGLVTNGERYN
                     KVVDIWGRAGDKIAKAMMDNLSKQKVIDRAGNEVDQESFNSIYMMADSGARGSAAQIK
                     QLSGMRGLMAKPDGSIIETPITSNFREGLTVLQYFIATHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVVEDDCGTSDGFVMKAVVQGGDVIEALRDRILGRVTASDVVDPSSG
                     ETLVEAGTLLTEKLVDMIDQSGVDEVKVRTPITCKTRHGLCAHCYGRDLARGKLVNAG
                     EAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASQVEAKSNGTARFSSQMRYVANN
                     KGELVVIGRSCEVVIHDDIGRERERHKVPYGAILLVQDGMAIKAGQTLATWDPHTRPM
                     ITEHAGMVKFENVEEGVTVAKQTDDVTGLSTLVVIDGKRRSSSASKLLRPTVKLLDEN
                     GVEICIPGTSTPVSMAFPVGAVITVREGQEIGKGDVLARIPQASSKTRDITGGLPRVA
                     ELFEARVPKDAGMLAEITGTVSFGKETKGKQRLIVTDVDGVAYETLISKEKQILVHDG
                     QVVNRGETIVDGAVDPHDILRLQGIEALARYIVQEVQEVYRLQGVKISDKHIEVIIRQ
                     MLRRVNIADAGETGFITGEQVERGDVMAANEKALEEGKEPARYENVLLGITKASLSTD
                     SFISAASFQETTRVLTEAAIMGKQDELRGLKENVIVGRLIPAGTGLTYHRSRHQQWQE
                     VEQETAETQVTDE"
     gene            complement(8375..12553)
                     /gene="rpoB"
                     /locus_tag="ACFM8S_000702"
     CDS             complement(8375..12553)
                     /gene="rpoB"
                     /locus_tag="ACFM8S_000702"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFC6313808.1"
                     /translation="MSYSFTEKKRIRKSFAKRENVLEVPFLLATQIDSYAKFLQLENA
                     FDKRTDDGLQAAFNSIFPIVSHNGYARLEFVHYTLGEPLFDIPECQLRGITYAAPLRA
                     RIRLVILDKEASKPTVKEVRENEVYMGEIPLMTPSGSFVINGTERVIVSQLHRSPGVF
                     FEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDLLYFRIDRRRKMPVTILLKALG
                     YNNEQILDIFYDKETFYLSSNGVQTDLVADRLKGETAKVDILDKEGNVLVAKGKRITA
                     KNIRDITNAGLTRLDVEPESLLGKALAADLIDSETGEVLASANDEITEELLAKFDING
                     VKEITTLYINELDQGAYISNTLRTDETAGRQAARVAIYRMMRPGEPPTEEAVEQLFNR
                     LFFSEDSYDLSRVGRMKFNTRTYEQKLSEAQQNSWYGRLLNETFAGAADKGGYVLSVE
                     DIVASIATLVELRNGHGEVDDIDHLGNRRVRSVGELTENQFRSGLARVERAVKERLNQ
                     AESENLMPHDLINAKPVSAAIKEFFGSSQLSQFMDQTNPLSEVTHKRRVSALGPGGLT
                     RERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYARTNDYGFLETPYRRVIDG
                     KVTEEIDYLSAIEEGRYVIAQANADLDSDGNLIGDLVTCREKGETIMATPDRVQYMDV
                     ATGQVVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPMVGTGIERSVAVDSA
                     TAIVARRGGVVEYVDANRVVIRVHDDEATAGEVGVDIYNLVKFTRSNQSTNINQRPAV
                     KAGDVLQRGDLVADGASTDLGELALGQNMTIAFMPWNGYNYEDSILISEKVAADDRYT
                     SIHIEELNVVARDTKLGAEDITRDIPNLSERMQNRLDESGIVYIGAEVEAGDVLVGKV
                     TPKGETQLTPEEKLLRAIFGEKASDVKDTSLRMPTGMSGTVIDVQVFTREGIQRDKRA
                     QSIIDSELKRYRLDLNDQLRIFDNDAFDRIERMIVGQKANGGPMKLAKGSEITTEYLA
                     GLPSRHDWFDIRLTDEDLAKQLELIKVSLQQKREEADELYEIKKKKLTQGDELQPGVQ
                     KMVKVFIAIKRRLQAGDKMAGRHGNKGVVSRILPVEDMPYMADGRPVDIVLNPLGVPS
                     RMNIGQILEVHLGWAAKGIGERIDRMLKEQRKAGELREFLNRLYNGSGKKEDLDALTD
                     EEIIELASNLRKGASFASPVFDGAKESEIREMLNLAYPSDDPEVEKLGFNDSKTQITL
                     YDGRSGEAFDRKVTVGVMHYLKLHHLVDEKMHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYENIVKGEHKIDAGMPESFNVLVK
                     EIRSLGLDIDLERY"
     gene            complement(12744..13115)
                     /gene="rplL"
                     /locus_tag="ACFM8S_000703"
     CDS             complement(12744..13115)
                     /gene="rplL"
                     /locus_tag="ACFM8S_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFC6313809.1"
                     /translation="MAITKEDILEAVGSLTVMELNDLVKAFEEKFGVSAAAVAVAGPA
                     GAGAADAEEKTEFDVVLASAGDQKVGVIKVVRAITGLGLKEAKDIVDGAPKTIKEGVS
                     KAEAEDIQKQLEEAGAKVEIK"
     gene            complement(13173..13673)
                     /gene="rplJ"
                     /locus_tag="ACFM8S_000704"
     CDS             complement(13173..13673)
                     /gene="rplJ"
                     /locus_tag="ACFM8S_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFC6313810.1"
                     /translation="MSLNIETKKVAVEEISAAIANAQTLVVAEYRGISVSSMTELRAN
                     ARKEGVYLRVLKNTLARRAVQGTSFAELADQMVGPLVYAASEDAVAAAKVLHQFAKKD
                     DKIVVKAGSYNGEVMNAAQVAELASIPSREELLSKLLFVMQAPVSGFARGLAALAEKK
                     AGEEAA"
     gene            complement(13903..14598)
                     /gene="rplA"
                     /locus_tag="ACFM8S_000705"
     CDS             complement(13903..14598)
                     /gene="rplA"
                     /locus_tag="ACFM8S_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFC6313811.1"
                     /translation="MAKVSKRLKALRSSVEANKLYAIDEAIALVKKAATAKFDESVDV
                     SFNLGVDPRKSDQVIRGSVVLPKGTGKITRVAVFTQGANAEAAKEAGADIVGFEDLAA
                     EIKAGNLNFDVVIASPDAMRIVGQLGTILGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VQYRTDKAGIVHATIGRASFAEADLKENFDALLDAIVKAKPAAAKGQYLKKVAVSSTM
                     GLGIRVDTSSVNN"
     gene            complement(14598..15032)
                     /gene="rplK"
                     /locus_tag="ACFM8S_000706"
     CDS             complement(14598..15032)
                     /gene="rplK"
                     /locus_tag="ACFM8S_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFC6313812.1"
                     /translation="MAKKIIGYIKLQIPAGKANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATQGMEPGLPIPVVITAFADKSFTFLMKTPPASILLKKAAGLQKGSSNPLTNKVGKLT
                     RAQLEEIAKTKDPDLTAADLDAAVRTIAGSARSMGLDVEGVV"
     gene            complement(15133..15669)
                     /gene="nusG"
                     /locus_tag="ACFM8S_000707"
     CDS             complement(15133..15669)
                     /gene="nusG"
                     /locus_tag="ACFM8S_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFC6313813.1"
                     /translation="MSKKWYVVQAYSGFEKNVQRILEERIAREEMGDYFGQILVPVEK
                     VVDIRNGRKTISERKSYPGYVLVEMEMTDDSWHLVKSTPRVSGFIGGRANRPTPISQR
                     EAEIILQQVQTGIEKPKPKVEFEVGQQVRVNEGPFADFNGVVEEVNYERNKLRVSVQI
                     FGRETPVELEFSQVEKIN"
     gene            complement(15671..15949)
                     /gene="secE"
                     /locus_tag="ACFM8S_000708"
     CDS             complement(15671..15949)
                     /gene="secE"
                     /locus_tag="ACFM8S_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFC6313814.1"
                     /translation="MTEHTPEKKNVKVDQLVVQDKESASNSGKEGFFAYFSNSWSEFK
                     KVVWPKREDAVRMTVFVIVFVAVLSIFIYAADTAISWLFFDVLLRREG"
     gene            complement(16066..16141)
                     /locus_tag="ACFM8S_000709"
     tRNA            complement(16066..16141)
                     /locus_tag="ACFM8S_000709"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16106..16108),aa:Trp,seq:cca)
     gene            complement(16148..>16522)
                     /locus_tag="ACFM8S_000710"
     CDS             complement(16148..>16522)
                     /locus_tag="ACFM8S_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFC6313815.1"
                     /translation="AGDNVGVLLRGTKREDVERGQVLAKPGTITPHTKFKAEVYVLSK
                     EEGGRHTPFFANYRPQFYFRTTDVTGAVTLEEGVEMVMPGENVTITVELIAPIAMEEG
                     LRFAIREGGRTVGAGVVSSVIA"
BASE COUNT         4158 a         4282 c         3692 g         4390 t
ORIGIN      
        1 gctaattcct ttacattaaa gatcgattaa agaacaatgc cgtctgaaaa atcatttcag
       61 cctttcggac ggcctatttg cctgattagc ctttacgggc ttcagttaca gcagcagcta
      121 tgtgggcagg agcttcagaa tatttcttga actccataga gtaagtagcg cggccttggg
      181 ttgcagaacg caggtcggtc gaataaccga acatttctgc cagaggtact tcggcacgga
      241 cttttttacc gccgataccg tcatcatcca tacccaatac aacaccgcga cggcggttca
      301 agtcgcccat tacgtcgccc atgtattctt ccggggtttc cacttcaact gccataatcg
      361 gctcaagcag ggcgggagat gcttgacgca taccttcttt aaacgcttga gaagcagcca
      421 attcaaatgc caattgggaa gagtcgacat catggtaaga accgaatacc agacggatac
      481 gtacgtcaac tacaggatag ccggcaacga taccgttagg caacgtatcg cggatacctt
      541 tatcgacaga cggaataaat tcgcgaggaa tcacaccacc tttaatttca tcgataaact
      601 cgtaaccttc accacccggt tccataggtt ccatttcaat cacaacgtga ccgtattgac
      661 ctttaccacc ggattgtttt gcatgtttgt attcggcttt aacggctttg cggatagttt
      721 cacggtaagc cacttgaggc gcaccgatat ttgcttccac accgaattcg cgtttcatac
      781 ggtcaacaat aatttccaag tgcagctcac ccataccgga aataatggtt tgaccggatt
      841 cttcgtctgt acggacacgg aaagaagggt cttctttagc caagcggttc agggcgatac
      901 ccattttctc ttggtcggct ttggttttcg gctcaacggc aatatggatt accggctcgg
      961 ggaattccat acgttccaag ataatcggcg cgctttccgc acacaaggtt tcaccggtag
     1021 taacgtcttt cagaccaata gcggctgcga tgtcgccggc gcgtacttct tcgatttcag
     1081 tacggtctgc ggcagtcatt tgtaccaaac gaccgatacg ttcgcgagtg ccttttacgg
     1141 agttcaatac ggtatcgccg gattttacta cgcctgagta aacgcggata aaggtcagct
     1201 gaccgacgta tttgtcgttc aacattttga acgccaatgc agagaatttc tcttcatcgc
     1261 tggcttgacg gctgtcggct tcctcggtat tcgggttgac accttgaacc ggaggaatat
     1321 cggtaggagc tggcagcaat tctacaactg cgtccaacat acgttgaaca cctttgtttt
     1381 taaatgcaga accacacagc ataggctgaa tttcgcctgc caaagtacgt tgacgcaacg
     1441 cgcctacgat ttcttcttcg gtcagctcgt cgccgcctaa gtatttgtcc atcagttctt
     1501 cgctggcttc ggctgcggct tcaatcatat tttgacgcca ttcttcggca gtttcgacca
     1561 attcggcagg aatatcgcca taggtaaagg ttgtaccttt atcgacttca ttccaaatga
     1621 tggatttcat tttcaacaaa tcaaccacac cgctgaagtt gtcttccgca ccaaccggaa
     1681 tgacgatagg tacagggttt gcgcgcaaac gggttttcat ttgctcgaca acgcggaaga
     1741 agttggcacc ctgacggtcc attttattga caaacgccaa gcgcggcact tggtatttgt
     1801 tggcttgccg ccatacggtt tcagattggg gttgaacacc gcccaccgcg cagtaaacca
     1861 ttaccgcgcc gtccaataca cgcatagaac gctctacctc tacggtaaag tcaacgtgtc
     1921 ccggggtgtc gatgatgttg aagcggtgct cggggaattg tttcgccata ccggaccagt
     1981 aggaagtaac ggcagcggag gtaatggtaa taccgcgctc ttgctcctgt tccatgtagt
     2041 cggtagtagc cgcaccgtca tgcacttcgc ccagcttgtg ggtcaaaccg gtatagaaca
     2101 aaatacgttc tgtcgtcgtg gttttacccg catcgatatg ggcggaaata ccgatgttac
     2161 ggtacaggct gatcggggtc ttacgagcca ttttattagc ctttcaaaat tagaaacgga
     2221 agtgagagaa tgctttgttg gcttcagcca tacggtgtac ttcttcacgt tttttcaacg
     2281 caccgccacg gccttcggac gcatcaatca attcgcctgc caaacgcagg tccatggatt
     2341 tctcaccacg tttgcgggcc gcatcgcgaa cccagcgcat tgccaaagcc aaacggcgtg
     2401 aaggacgaac ttcaacagga acttggtagt ttgcaccacc tacacggcgg cttttcactt
     2461 ccacgatagg tttggcgttt gcaatggctt cgttaaatac ttcgattgct actttgccgg
     2521 tttttttctc aatctgttcc aacgcaccgt aaacgatacg ctcggcaacg gattttttac
     2581 cgtcaatcat caatacgttc atgaatttgg tcaactcgac gctgccgaat ttaggatctg
     2641 gcagtacgtc gcgcttgggg acttctctac gtcttggcat tttaatttcc tttaatctat
     2701 tcagtcgggt ctattcccat gaatacccaa ttgagtattc acttactcgg ccgttgttca
     2761 gcttaggcgg ccgacgtgcc tatttaagtc ccagtaatta tttaggacgc ttagcaccgt
     2821 atttggaacg ggcttgttta cggtctttaa cgcctgcagt atccaaagaa ccgcgtacag
     2881 tgtggtaacg cacacctggc aagtctttta cacgaccgcc gcgaatcaat acgacactgt
     2941 gctcttgcag gttgtggcct tcgccgccga tgtatgaaat gacttcaaaa ccgttggtca
     3001 ggcggacttt acatacttta cgcaatgcag agttaggttt tttaggggta gtcgtgtata
     3061 cacgggtgca cacgccgcgt ttttgcgggc aggcttccag tgcgggcact ttgtttacgt
     3121 acacgggctt ttgacggcct ttgcgtacca attggttgat agttggcata ttttctcgtc
     3181 ctgttgagtt aaatacttgc cgacaccatg tcggcaagag tggaattata tttttattgc
     3241 aagcagacag tcaagcataa cgacaagaag aaagatgatt ttcttgtttg ccgataaata
     3301 tttaccgaca tccctcacaa tcagtcggac gactgaagaa aatactgtca atacgttttt
     3361 tctatactga caaaccgttc cttgccgctg cgcgaataaa aggcgggcat attgctgttg
     3421 atcttgttgt aacggaaatt tatttggggt gtaaatcctt tccacgacag ttttttatgc
     3481 cataaagccg cgctcatccg atgttaatcg gcattctcat ccggtcaccc cgaacatcgc
     3541 cgcctgccgc gcttttagga acttcagacg gcattgcatc gcgaaccggc ggtacatcct
     3601 cggcaaacgg caggagggcg aggctttccc cttcagacgg catcgcaatc cgattaccga
     3661 cgttaatcag ttcgacaggt acgccgatat cctcggcaaa cgtcgccgaa gtcagcaggc
     3721 agcctgcaaa aaagagattt ctgggtttca tatttatttt ttaaaggaaa tgccgtctga
     3781 agtttcagac gacatttgtt taaatggaaa acattaagac gctacttttt ttgaccgccg
     3841 aaaccaacat ccctggcaaa gttattttgg tctccggacg taccgacgac acccgccacc
     3901 tcgttggtct ccggcccgaa aatacctaag gaataatcca cacgcctcaa aatgacgaaa
     3961 acgcttgttt ttgccttaat cggacaaaga aaaaccgcaa gcttaaagct tgcggttttg
     4021 tattgaataa acgcaccaaa gattattcat ccgttacttg ggtttcagca gtctcctgtt
     4081 ccacctcttg ccattgttga tgacggctgc ggtggtaagt caaaccggta ccggcaggaa
     4141 tcaagcgacc gacgatgacg ttttctttca aaccacgcaa ctcgtcttgt ttgcccataa
     4201 tcgcggcttc ggtcagaacg cgggtcgttt cttggaacga tgcggcagaa atgaagctgt
     4261 cggtggacag ggaagctttg gtaataccca gcaatacgtt ttcgtaacgc gccggttctt
     4321 tgccttcttc caaagctttt tcattggccg ccatcacatc gccgcgttcg acctgctctc
     4381 cggtaatgaa cccggtttcg ccggcatccg caatgttcac acggcgcaac atttgacgga
     4441 tgatgacttc gatgtgttta tcagaaatct tcacaccttg cagacggtaa acctcttgca
     4501 cctcttggac aatgtagcgt gccagtgctt cgataccttg caaacgcaga atatcgtgcg
     4561 gatcgaccgc gccgtccacg atggtttcac cgcggtttac cacttgaccg tcgtgtacca
     4621 gaatttgttt ctctttggaa atcaaggtct cgtatgctac accgtccacg tcagtaacaa
     4681 tcagacgttg cttgcctttg gtctctttgc cgaaggaaac ggtaccggta atttccgcca
     4741 acataccggc atctttcggc acgcgtgctt caaacaattc ggcaacgcgc ggcagaccgc
     4801 cggtaatgtc gcgggttttg gaagaggctt gcggaatacg cgccaatacg tcgcctttac
     4861 cgatttcctg accttcgcgt acggtaatca ccgcaccaac ggggaatgcc atggataccg
     4921 gagtagaagt accgggaata cagatttcca cgccgttttc gtccaagagt ttcacagtcg
     4981 gacgcagcag tttggaagca ctagaggaac gacgtttacc gtcaatcacc accaaagtgg
     5041 acaaaccggt tacatcatcg gtttgtttgg caacggtaac gccctcttcc acgttttcga
     5101 atttcaccat acctgcgtgt tcggtaatca tcggacgggt atgcggatcc caggttgcca
     5161 aggtttgacc ggctttaatg gccataccgt cttgtaccag caggatggca ccgtaaggta
     5221 ctttgtggcg ttcgcgttca cggccgatat cgtcgtgaat cacgacttca caagaacggc
     5281 cgatgacaac caactcgcct ttattgttgg caacataacg catttggctg ctgaatcgtg
     5341 ccgtaccgtt ggatttggct tccacttggc tggctgctgc cgcacgggat gccgcaccac
     5401 cgatgtggaa cgtacgcata gtcagctggg tacccggttc gccgattgat tgggcggcaa
     5461 tcacgccgac tgcctctccg gcattgacca gtttgccgcg cgccaagtcg cggccgtaac
     5521 agtgcgcgca caaaccgtgg cgggttttac aagtaatcgg cgtacggact ttgacttcat
     5581 cgacaccgga ttggtcaatc atatccacca gtttttcagt caacaacgtg cctgcttcaa
     5641 ccaaggtctc gccgcttgac ggatcgacaa cgtcagacgc agtaacacgg cccaaaatac
     5701 gatcgcgcaa tgcttcaatc acatcaccgc cttgtaccac tgccttcatg acaaagccgt
     5761 ctgaagtacc gcaatcgtct tcaacaacga ccaaatcttg agttacgtct accagacgac
     5821 gggtcaggta accggagttc gcggttttca atgcggtatc cgccaaaccc ttacgcgcac
     5881 cgtgggtcgc aataaagtat tgcaatacgg tcaggccttc gcggaagttt gaggtaatcg
     5941 gcgtttcaat aatcgagccg tcaggtttag ccatcaaccc gcgcataccg gacaactgtt
     6001 taatctgagc cgcagaacca cgggcaccgg agtccgccat catataaatg gagttgaatg
     6061 actcttgatc gacttcgttg ccggcacggt cgataacttt ttgtttggac aagttatcca
     6121 tcatcgcttt agcgatttta tcgccggcac gaccccaaat atccactact ttgttgtaac
     6181 gttcgccatt ggtaaccaaa ccttgacggt attggtcttc gatttcttta acctcggcat
     6241 tggcttcagc cagcaaggcc gctttttctt tcgggatttc catatcgtca acggcaatgg
     6301 aaataccgcc ttttgccgca aatccgaaac cggtgtacat caggtggtca gcaaagataa
     6361 ccgtatcgcg caagccgcac aggcggaacg atgcgttaat cagtttagaa atttcttttt
     6421 tcttcaatgc cttgttgaca tattcaaacg gcaggccttt cggcaggatt tcgctcaaca
     6481 atgcacggcc gacggttgtt tcgtaacggt taacgacagg ttcaaactca cctgcttcgt
     6541 ttttcaccca ttcgcgcaga cgtacggtaa ttttcgtacc cagctcgacc tgtttggtat
     6601 ggtatgcacg atgcacttct ttcacatcgg caaacaggct gccttcgcct ttggcattga
     6661 tacggtcgcg ggtcatgtag tacaaaccca atacgatgtc ttgggaaggt acgataatcg
     6721 gttcgccgtt ggctggagac aatacgttgt ttgaagccag catcagggtg cgcgcttcca
     6781 tttgtgcttc caagctcaat ggaacgtgta cagccatttg gtcaccgtca aagtcggcgt
     6841 taaatgcggc acacaccaat gggtgtaact gaatcgcttt accttcaatc aggataggtt
     6901 cgaacgcttg aatacccaaa cggtgcaggg ttggcgcacg gttcagcata atcggatgtt
     6961 cgcggatcac ttcttccaag atatcccata cttcaggtac ttcttgttct accagttttt
     7021 tcgcagcttt aacggtagaa gccaaacctt gtttttccaa tttgtggaaa atgaatggtt
     7081 tgaacaactc caaagccatt tttttcggca gaccgcattg gtgcagacgc aggtatgggc
     7141 ctacggtaat cacggaacgg ccggagtagt ccacacgttt acccagcagg ttttgacgga
     7201 agcgtccgcc tttaccttta atcatgtcag ccaatgattt cagcgggcgt ttgttggcgc
     7261 cggtcatggc tttaccgcga cggccgttat ccaacagcga gtcaactgct tcttgcaaca
     7321 tacgtttttc gttgcggacg ataatgtcgg gcgcgtgcag ttccagcagg cgtttcagac
     7381 ggttgttacg gttgatgacg cgacggtaca aatcgttcaa atcggaagtg gcaaaacgac
     7441 cgccatccaa tggaaccaac ggacgcaaat caggcggcaa taccggcagc acgtccataa
     7501 tcatccattc cagcttcata ccggaacggt ggaaggcttc caatactttc aaacgtttgg
     7561 cgattttttt gattttggtg tcggatccgg tcgattccaa ctcttggcgc aggatttcga
     7621 tttcgcccgc tacattcagg gtacgcagca attcgcggat accttccgca cccattttgg
     7681 catcgaaatc gtcgccgtat tcgtccagct tgttgtagta atcgtcttca gtcagcaatt
     7741 ggcggcgttg cagcggagtc ataccgggat cggttaccac aaatgcttca aagtacaata
     7801 cgcgctcgat gtcgcgcaaa gtcatgtcca gtaccatacc caagcgggaa ggcagggatt
     7861 ttaagaacca aatatgtgcg acgggcgcag ccaattcgat atggcccatg cgttcgcggc
     7921 gcactttgga cagggttact tccacgccgc atttttcaca cgttacgcct ttaaatttca
     7981 agcgtttgta ttttccgcac aagcattcgt agtctttgac cgggccaaag attttggcac
     8041 agaacaaacc gtcacgctca ggtttgaacg tacggtagtt gatggtttca ggttttttga
     8101 cttcgccata agaccatgag cggatggttt cgggagaggc aataccgatt ttaatggcat
     8161 caaactcttc ttccatgccg gcagtttgca acggattaaa taagttcaac aaattcattt
     8221 ttgctccttg aaggaagtat ttttaccgca tggcggattt tcgatatggg gaaacggtac
     8281 ttttgatttc agacggccta aatgccgatt gcttcattct tattctgaaa ccactttttc
     8341 agacgaccct gaaaggccgt ctgaaaactt ttgtttagta acgttccaaa tcgatatcca
     8401 agcccagtga gcgaatctct ttgaccaata cgttgaagga ctcgggcata ccggcatcga
     8461 ttttgtgttc gcctttgacg atgttttcgt acattttggt acggccgttc acgtcgtcag
     8521 acttcacagt cagcatctct tgcagcgtgt atgccgcgcc gtatgcttcc aatgcccaaa
     8581 cctccatctc gccgaaacgt tggccgccga actgggcttt accgcccaaa ggctgctggg
     8641 taaccagact gtacggaccg gtagaacgcg cgtgcatttt ttcgtcaacc aagtggtgca
     8701 gtttcagata gtgcatcaca cctactgtaa ccttgcggtc aaatgcttcg cctgaacggc
     8761 cgtcatacag cgtgatttgg gttttactgt cgttgaagcc cagtttttca acctcaggat
     8821 cgtcgctcgg ataagccaag ttcaacattt cgcggatttc agactctttc gcaccgtcga
     8881 atacaggaga ggcgaaagat gcacctttac gcaggttgga ggccagttcg atgatttctt
     8941 catcagtcag ggcatccaaa tcttctttct tgccgctgcc gttgtagagt ctgttcaaga
     9001 actcgcgcaa ctcgcctgct ttgcgttgct ctttcagcat acggtcgata cgctcgccga
     9061 taccttttgc tgcccaaccc aagtgaactt ccaaaatctg accgatgttc atacgggaag
     9121 gtacgcccaa tgggttcagt acgatgtcta ccggacggcc gtccgccatg taaggcatgt
     9181 cttccactgg cagaatgcgc gataccacgc ctttgttacc gtggcggccc gccattttgt
     9241 cgccggcttg cagacggcgt ttgatggcga taaaaacttt caccattttt tgtacgccgg
     9301 gttgcaattc gtcgccttgg gtcagttttt tcttcttgat ttcgtataac tcgtccgctt
     9361 cttcgcgttt ttgttgcagg ctcactttaa tcagttccaa ctgcttggcc aaatcttcat
     9421 cggtcagacg gatatcgaac caatcgtgcc tgctcggcag acccgccaga tattcggtcg
     9481 tgatttcact gcctttggcc agcttcatcg ggccgccgtt ggctttctga ccgacaatca
     9541 tacgctcgat acggtcgaat gcgtcgttgt cgaaaatacg caattggtcg ttcaaatcca
     9601 aacggtagcg tttcaactcg gaatcgataa tggattgagc acgtttgtcg cgttgaatac
     9661 cttcacgagt gaagacttga acgtcgataa cggtaccgct catgccggta ggcatacgca
     9721 atgaagtatc ttttacgtca gatgcttttt caccgaagat ggcgcgcagc agtttttctt
     9781 ccggcgtcag ctgggtttcg cctttaggcg ttaccttgcc taccaacaca tcgccggctt
     9841 ctacttccgc accgatgtaa acgataccgg attcgtccaa acggttttgc atacgctcgg
     9901 acaagttcgg aatatcgcgg gtaatgtctt ccgcacccag cttggtatcg cgggcaacga
     9961 cattcaattc ctcaatgtga atcgaagtat agcggtcgtc cgcagccact ttttcggaaa
    10021 tcagaatcga gtcttcgtag ttgtaaccgt tccacggcat gaaggcgatg gtcatatttt
    10081 gacccaaagc caattcgccc aaatcggtgg acgcgccatc ggccaccaaa tcgccgcgtt
    10141 gcaaaacatc gcctgctttg acagccggac gttggttgat gttagtagat tgattggaac
    10201 gggtaaattt aaccaagttg taaatatcga cacccacttc gccggcagtc gcttcgtcgt
    10261 catggacacg aatcacaaca cggttggcat cgacatactc gaccacgccg cctcggcggg
    10321 caacgattgc agtagcagag tcaacggcaa cggaacgctc gataccggta ccgaccatcg
    10381 gtttttcagg acgcaagcaa ggcactgcct gacgttgcat gttggcaccc atcaatgcgc
    10441 ggttcgcgtc atcatgttcc aagaacggaa tcagggatgc tgcaacggat accacttgac
    10501 cagttgccac gtccatatat tggacgcggt cgggcgttgc cataatggtt tcgccttttt
    10561 cacgacaggt aaccaaatcg ccaatcagat tgccatctga atccaaatcg gcattcgcct
    10621 gtgcaatcac atagcggcct tcttcgatgg cagacaagta atcgatttcc tcggttactt
    10681 tgccgtcgat aacgcggcgg taaggcgttt ccaagaaacc gtaatcattg gtgcgcgcat
    10741 aaacggacaa tgagttgatc aaaccgatgt tcggaccttc aggcgtttca atcggacata
    10801 cgcgaccgta gtgggtcgga tgcacgtccc gcacctcaaa tcctgcacgt tcgcgggtca
    10861 aaccgcccgg acccaatgcg gatacgcggc gtttatgggt tacttcagac aagggattgg
    10921 tctgatccat aaactgactc aattggctgg agccgaagaa ttctttaata gcggcagaaa
    10981 caggttttgc attaatcaaa tcgtgcggca tcaagttttc tgattccgcc tgattcaaac
    11041 gttcttttac ggcacgttcc acacgggcca aaccgctacg gaattggttt tcagtcagct
    11101 cgcctaccga acgtactcgg cggttgccca agtgatcgat atcgtccact tcgccatggc
    11161 cgttacgcaa ctcgaccaaa gtcgcaatcg aggcgacaat atcttcgacg ctcaggacat
    11221 aaccgccttt gtcggcagca ccggcaaacg tttcgttcag caggcggccg taccaagagt
    11281 tttgttgggc ttcggacagt ttttgttcgt atgtgcgcgt attaaatttc atacggccta
    11341 cgcgggacag atcgtagctg tcttcactga agaacaagcg gttaaacaat tgctcgaccg
    11401 cctcttcggt gggcggttcg cccggacgca tcatacggta aatcgcaaca cgagccgcct
    11461 gccggccggc agtctcatcc gtacgcaagg tattggagat ataagcaccc tgatccagct
    11521 cattgatata aagggtcgta atttctttta cgccgttgat atcaaatttg gccaacaact
    11581 cttctgtaat ttcatcattg gcagaagcca atacctcgcc ggtttccgaa tcaatcagat
    11641 cggcagccaa tgctttgccc agcaggcttt ccggttctac atccaaacgg gtcaggcctg
    11701 cattggtaat atcacggata tttttcgcag taatgcgctt acctttggca accaatacat
    11761 tgccttcttt atccaagata tcgaccttgg cagtttcgcc tttcagacgg tctgcgacca
    11821 aatcggtttg aacaccgttt gaagacaaat agaacgtttc tttgtcgtag aaaatatcca
    11881 agatttgctc attgttgtag cctaaagcct tcaacaaaat cgttaccggc attttacggc
    11941 ggcggtcgat acggaaatac agcaaatctt tcggatcaaa ttcaaaatcc aaccatgaac
    12001 cacggtaggg aatgatgcgg gcggagaata acaatttgcc ggaagagtgc gtcttacctt
    12061 tgtcatgctc gaagaatacg ccgggcgaac ggtgcaactg ggagacaatc acacgctctg
    12121 tgccgttaat cacaaaagaa ccgctcgggg tcatcaacgg aatttcgccc atatacactt
    12181 cgttttcacg aacttctttt accgtcggtt tagatgcttc cttatccaaa atcaccaaac
    12241 ggatacgcgc gcgcaagggg gctgcataag tgattccgcg caactgacat tcgggaatat
    12301 cgaacaaagg ctcgcccaat gtgtaatgca caaactccaa tcgcgcataa ccgttatggc
    12361 tcacaatcgg gaaaatagaa ttaaatgccg cctgcagacc gtcatcggta cgtttgtcaa
    12421 aagcattttc cagctgcaaa aacttcgcat aagaatcaat ttgggttgcc agcaagaaag
    12481 gaacttccaa aacattttcc cgctttgcaa aactcttacg gatacgtttt ttctcggtaa
    12541 acgaatagct catatacact ccgaaaagta attttcaata ataggccgtc tgaaagcaac
    12601 ggcacgcatc aatctgatgt ttacatttat ttgcaatgat ttgataaaaa ctatgcaaat
    12661 aaatgtaaac aatacttaat gcttatttta agaagcaaaa taaggctggc agaaaactgc
    12721 cagccttcat aagaagcatc aaattatttg atttcgactt tagcgccggc ttcttccagt
    12781 tgtttttgga tgtcttcggc ttcagcttta gaaacaccct ctttaatggt tttaggtgca
    12841 ccgtcaacga tgtctttagc ttctttcaga cccaaaccgg taattgcacg gacaacttta
    12901 atcacgccga ctttttgatc accggcagaa gccaaaacga catcaaattc ggttttttct
    12961 tcagcatcgg cagcaccggc accagcagga cctgcaactg caacagcagc agcagaaaca
    13021 ccgaattttt cttcaaaagc tttaaccaag tcgttcaatt ccattacggt caaagaacca
    13081 actgcttcca aaatgtcttc tttagtaata gccatgctat tttactccaa atattgtatt
    13141 aaaaaataat tgattaacag aaacaaaatc gattaagcgg cttcttcgcc ggcttttttc
    13201 tctgccaaag cagccaaacc gcgcgcaaag cccgatacag gagcttgcat aacgaacaac
    13261 agtttggaca acagctcttc gcggctcgga atagaagcca actcagcaac ctgagcagca
    13321 ttcattactt cgccattgta agaaccggct ttaacgacaa ttttgtcatc ttttttcgcg
    13381 aattggtgca acactttagc agcagcaaca gcatcttcag aagcagcgta aaccaacgga
    13441 ccaaccattt gatcggccaa ttctgcaaat gaagtacctt gcactgcacg acgagccaaa
    13501 gtatttttca gaacgcgcaa ataaacgcct tctttacgtg cattcgcacg aagctcagtc
    13561 atactggaaa cactgatacc gcgatattca gcgactacga gggtttgagc attagcaatt
    13621 gccgcgctaa tttcctcgac cgccactttc ttggtttcaa tattgagact caaggtctac
    13681 ctcccactgt ttataaacag gataccaaaa cgatatccca ccagcgcggt aacctaaaaa
    13741 gacatcttac aagcaatctt gcaatgtgtt tcaggactac cgtctgcgta gggcagttac
    13801 gattaaatct tacgatccct acggtcttgg acatctactt aattttaagt agcccaaatt
    13861 caagcagccc agaaaaccaa actgcttgaa aattccttaa gattagttat ttacgcttga
    13921 tgtatcaacg cgaataccca aacccatggt gctagacaca gcaacttttt tcagatactg
    13981 acctttagcg gcagcaggct tggctttaac gatagcatcc agcaacgcat caaagttctc
    14041 tttcaaatca gcttcagcga atgaagcacg accaatcgtt gcatgaacaa tacctgcctt
    14101 gtcggtacgg tactgcactt gacctgcttt tgcatttttt actgcttcgg caacattagg
    14161 ggtaaccgta cctactttag ggtttggcat cagaccacga ggacccaaga tggtacccaa
    14221 ttgaccaaca atacgcattg catcgggaga agcaataaca acgtcaaagt tcagattgcc
    14281 tgctttgatt tcagcagcca aatcttcgaa accgacgata tctgcaccag cttctttagc
    14341 agcttctgca tttgcacctt gagtaaatac agccacacgg gttatcttac cggtgccttt
    14401 aggcagaacg accgaaccac ggataacttg gtcagattta cgcggatcaa cgcccaagtt
    14461 gaaagatacg tcaacagact cgtcaaattt agcagtcgct gcttttttta ccaaagcaat
    14521 tgcttcatca attgcatata atttattggc ttccacagaa gagcgaagag ctttcaagcg
    14581 tttagatact ttagccatta tacaacaccc tccacatcca agcccattga gcgagcagaa
    14641 cctgctatag tacggacagc cgcatccaag tcagcagcag tcaaatcagg atctttagtt
    14701 ttagcaattt cttccaactg ggcacgggtc aatttaccca ctttgttggt cagaggatta
    14761 gaactacctt tttgcaaacc ggcagccttt ttcaacaaga tagaagctgg cggggttttc
    14821 atcaaaaatg tgaatgattt atctgcaaat gcagtaatca caaccggaat cggtaagcca
    14881 ggctccatac cttgggttgc agcattaaat gccttacaaa attccataat attcaaaccg
    14941 cgctgaccca aagcaggacc aaccggagga gatggattgg ctttacctgc aggaatttgc
    15001 agtttaatat agccgataat tttctttgcc acttaacgaa ctcctaaaaa cgggtaagac
    15061 gcggaaccat ttccgctccc caagttaatt tttgattcta tcctcttatt gctttttttt
    15121 caagtataaa aatcagttaa tcttttcaac ctggctgaac tccagctcaa cgggtgtttc
    15181 tctaccaaat atctgaacag acacgcgtaa cttattccgt tcataattga cctcctcaac
    15241 caccccgtta aaatccgcaa acggcccttc atttacacga acctgttgac cgacctcaaa
    15301 ttcaactttt ggtttcggct tctctatgcc ggtctgaacc tgctgtaaaa taatttcagc
    15361 ctctctctga ctaatcggcg taggtctatt agccctccct ccaataaaac cggaaacacg
    15421 gggggtgctt tttacaagat gccaagagtc atctgtcatt tccatctcaa ctagcacata
    15481 accaggatat gactttcttt cactaatagt cttacgacca ttgcggatat caacaacttt
    15541 ctctacaggc accagaattt gtccgaaata atctcccatc tcctcacggg caatgcgctc
    15601 ttccaatatt cgttggacat tcttctcaaa ccccgaatac gcctgtacaa cataccattt
    15661 tttcgacatc tcaaccttcc cttctcagca atacatcaaa aaataaccac gaaattgctg
    15721 tatctgccgc atagataaat atagaaagca cagcaacaaa cactataaca aatacagtca
    15781 ttctgacagc atcttcacgc ttaggccaaa ccaccttttt gaattcggac caagaatttg
    15841 agaaatatgc aaaaaaccct tccttaccgg aattagatgc agattcttta tcttgaacaa
    15901 ccagttgatc cactttaacg ttcttttttt caggcgtatg ttctgtcata ttatttatcc
    15961 atcatagcat ctgtctattc tcaatccatg taaatggcaa gagagtttac taaataacaa
    16021 atacaaaaaa aaattaaccg gcacaaggcc ggttaatttt ttatttggca ggccaagagg
    16081 gtctcgaacc cccaaccctc ggttttggag accgatactc taccaattga gctattggcc
    16141 tctaaactta agcgataaca gaagaaacca cgccggcacc cacggtacgg ccgccttcgc
    16201 gaatcgcaaa gcgcaaacct tcttccatag cgataggcgc aatcagttct acggtgatgg
    16261 ttacgttctc gcccggcatt accatttcca caccttcttc caaagtaacc gcgccggtta
    16321 cgtcggtggt acggaagtag aattggggac ggtagttggc gaagaacggg gtatggcggc
    16381 cgccctcttc tttgctcagt acgtatactt ctgctttgaa cttggtgtga ggagtgattg
    16441 tacccggttt agccaatact tgaccacgct ctacgtcttc acgtttggta ccgcgcagca
    16501 atacgcctac gttgtcgcct gc
//
LOCUS       DAXHSF010000028        15844 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.028, whole genome shotgun sequence.
ACCESSION   DAXHSF010000028 DAXHSF010000000
VERSION     DAXHSF010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15844
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.028"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(57..677)
                     /gene="galE"
                     /locus_tag="ACFM8S_000711"
                     /pseudo
     CDS             complement(57..677)
                     /gene="galE"
                     /locus_tag="ACFM8S_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248656.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
     gene            complement(756..1100)
                     /locus_tag="ACFM8S_000712"
     CDS             complement(756..1100)
                     /locus_tag="ACFM8S_000712"
                     /inference="COORDINATES: protein motif:HMM:NF014177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFC6313816.1"
                     /translation="MNKQYPKINYIGNKEKIASWICDQLPSDVDTVADVFSGGCSFAY
                     EAKKRGYRVITNDILAINYQIALALIENNHETLNDDDGAMIFSGSPHAGFMSQRYAEK
                     FYFHDEYQQLDL"
     gene            complement(1093..1287)
                     /locus_tag="ACFM8S_000713"
     CDS             complement(1093..1287)
                     /locus_tag="ACFM8S_000713"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC6313817.1"
                     /translation="MRKVFHYEQNRALTVRELAALQSFPDNFIFCGSKIAQQQQVGNA
                     VPPLLAKAIAESILKMSENE"
     gene            complement(1265..2095)
                     /locus_tag="ACFM8S_000714"
     CDS             complement(1265..2095)
                     /locus_tag="ACFM8S_000714"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC6313818.1"
                     /translation="MHTPLTYIDLFSGAGGLSLGFEQAGFQQLLSVEMESDYCQTYRT
                     NFPHHQLLQKDLTTLTEQDLINCLNGQAVDLIIGGPPCQGFSMAGKIGRTFTDDPRNH
                     LFKEFVRIVKIVQPYFFVMENVARLYTHNSGKTRIEIIQAFQNIGYSVECKILSAADF
                     GVPQIRSRVIFIGRRDKGKISFPEPLQISHQTVGSAIGHFPKLAAGESNPHVANHEAM
                     NHSAQMLEKMAFVKNGGNRNDIPEPLRPKTGDIRKYIRYNSNKTSRLYYRRYAQSFSL
                     "
     gene            complement(2126..4399)
                     /locus_tag="ACFM8S_000715"
     CDS             complement(2126..4399)
                     /locus_tag="ACFM8S_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFC6313819.1"
                     /translation="MNITQILSQELSATAAQITAAVELLDDGATVPFIARYRKEATGG
                     LDDTQLRRLAERLQYLRELEERKAVVLKSIEEQGKLSDGLRAQIEAADNKTALEDLYL
                     PYKPKRRTKAQIAREHGLQPLADVLLAEQSQDVEAAAQGYLNENVPDAKAALDGARAI
                     LMEQFAEDAELIGTLRDKLWNEAEIHAQVVGGKETEGEKFSDYFDHREPIRAMPSHRA
                     LAVLRGRNEGVLNIALKYQPDDTPITQQSEYEQIIARRFKVSDGHKWLRDTVRLTWRA
                     KIFLSLELEALNRLKEAADTDAITVFARNLKDLLLAAPAGRLTTLGLDPGYRNGVKCA
                     VVDDTGKLLDTVIVYLHQENNMLATLSRLIKQHGVKLIAIGNGTASRETDKIAGELVR
                     EMSKMGLHKIVVSEAGASIYSASELAAREFPDLDVSLRGAVSIARRLQDPLAELVKID
                     PKSIGVGQYQHDVNQSQLAKSLDAVVEDCVNAVGVDVNTASAPLLARISGLNQTLAQN
                     IVAYRDENGAFDSRKKLLKVPRLGEKTFEQAAGFLRINGGKEPLDASAVHPEAYPVVA
                     KMLAQQGISAAELIGNRERVKQIKASDFTDERFGLPTILDILSELEKPGRDPRGEFQT
                     ASFAEGIHEISDLQVGMILEGVVSNVANFGAFVDIGVHQDGLVHISALSNKFVQDPRE
                     VVKAGDVVKVKVLEVDAARKRIALTMRLDDEPGGAKHKMPSENRSRERTAGRKPQRND
                     RAPANSAMADAFAKLKR"
     gene            complement(4463..5113)
                     /locus_tag="ACFM8S_000716"
     CDS             complement(4463..5113)
                     /locus_tag="ACFM8S_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC6313820.1"
                     /translation="MISVEHVSKRYLTRQGWRTVLHDISFKMEKGEKIGILGRNGAGK
                     STLIRLISGVEPPTTGEIKRTMSISWPLAFSGAFQGSLTGMDNLRFICRIYNVDIDYV
                     KNFTEEFSELGQYLYEPVKRYSSGMKARLAFALSLAVEFDCYLIDEVIAVGDSRFADK
                     CKYELFEKRKDRSIILVSHSHSAMKQYCDNAMVLEKGHMYQFEDMDKAYEYYNSLP"
     gene            complement(5110..5907)
                     /locus_tag="ACFM8S_000717"
     CDS             complement(5110..5907)
                     /locus_tag="ACFM8S_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC6313821.1"
                     /translation="MKALHKTSFWESLAIQRRVIGALLMREIITRYGRNNIGFLWLFV
                     EPLLMTFVIVLMWKFLKADRYSTLNIVAFAITGYPMLMMWRNASKRAVGSISSNASLL
                     YHRNVRVLDTILARMILEIAGATIAQIVIMAVLIAIGWIEMPADMFYMLMAWLLMAFF
                     AIGLGLVICSIAFNFEPFGKIWGTLTFVMMPLSGAFFFVHNLPPKVQEYALMIPMVHG
                     TEMFRAGYFGDSVTTYENPWYILLCNLVLLLLGLAVVARFSKGVEPQ"
     gene            complement(5907..7070)
                     /gene="ctrB"
                     /locus_tag="ACFM8S_000718"
     CDS             complement(5907..7070)
                     /gene="ctrB"
                     /locus_tag="ACFM8S_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export protein CtrB"
                     /protein_id="HFC6313822.1"
                     /translation="MSEQLPVAVATETKAERKKPKKKSWIKKLSPLFWVTVIIPTVIS
                     LVYFGFFASDRFTSQSSFVVRSPKSQSSLNGLGAILQGTGFARAQDDIYTVGEYMRSR
                     SSLDELRKILPVREFYETKGDAFSRFNGFGFRGEEEAFYQYYKNQVMINFDTVSGIST
                     LNVTSFDALESKKINEALLKQGEALINQLNDRARADTVRYAEEVVKTAAERVKEASQN
                     LTDYRIANGVFDLKAQSEVQMGLVSKLQDELIVIQTQLDQVKAVTPENPQIPGLQARE
                     QSLRKEIDQQLRAISGGGHSSLSNQAAEYQRVYLENQLAEQQLAAAMTSLESAKVEAD
                     RQQLYLEVISQPSLPDLAHEPKRLYNIVATLIIGLMVYGILSLLTASIREHKN"
     gene            complement(7085..8260)
                     /gene="ctrA"
                     /locus_tag="ACFM8S_000719"
     CDS             complement(7085..8260)
                     /gene="ctrA"
                     /locus_tag="ACFM8S_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export outer membrane
                     protein CtrA"
                     /protein_id="HFC6313823.1"
                     /translation="MFKVKFYIRHAVLLLCGSLIVGCSAIPSSGPSAKKIVSLGQQSE
                     VQIPEVELIDVNHTVAQLLYKAQINQSFTQFGDGYASAGTLNIGDVLDIMIWEAPPAV
                     LFGGGLSSMGSGSAHQTKLPEQLVTARGTVSVPFVGDISVVGKTPGQVQEIIKGRLKK
                     MANQPQVMVRLVQNNAANVSVIRAGNSVRMPLTAAGERVLDAVAAVGGSTANVQDTNV
                     QLTRGNVVRTVALEDLVANPRQNILLRRGDVVTMITNPYTFTSMGAVGRTQEIGFSAR
                     GLSLSEAIGRMGGLQDRRSDARGVFVFRYTPLVELPAERQDKWIAQGYGSEAEIPTVY
                     RVNMADAHSLFSMQRFPVKNKDVLYVSNAPLAEVQKFLSFVFSPVTSGANSINNLTN"
     gene            8395..9528
                     /gene="neuC"
                     /locus_tag="ACFM8S_000720"
     CDS             8395..9528
                     /gene="neuC"
                     /locus_tag="ACFM8S_000720"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFC6313824.1"
                     /translation="MKRILCITGTRADFGKLKPLLAYIENHPDLELHLIVTGMHMMKT
                     YGRTCKEVTRENYQHTYLFSNQIQGEPMGAVLGNTITFISRLSDEIEPDMVMIHGDRL
                     EALAGATVGALSSRLVCHIEGGELSGTVDDSIRHSISKLSHIHLVANEQAVTRLVQMG
                     EKRKHIHIIGSPDLDVMASSTLPSLEEVKEYYGLPYENYGISMFHPVTTEAHLMPQYA
                     AQYFKALELSGQNIISIYPNNDTGTESILQELLKCQSEKFIAFPSIRFEYFLVLLKHA
                     KFMVGNSSAGIREAPLYGVPSIDVGTRQSNRHMGKSIIHTDYETKNIFDAIQQACSLG
                     KFEADDTFNGGDTRTSTERFAEVINNPETWNVSAQKRFIDLNL"
     gene            9532..10218
                     /gene="neuA"
                     /locus_tag="ACFM8S_000721"
     CDS             9532..10218
                     /gene="neuA"
                     /locus_tag="ACFM8S_000721"
                     /EC_number="2.7.7.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="HFC6313825.1"
                     /translation="MEKQNIAVILARQNSKGLPLKNLRKMNGISLLGHTINAAISSKC
                     FDRIIVSTDGGLIAEEAKNFGVEVVLRPAELASDTASSISGVIHALETIGSNSGTVTL
                     LQPTSPLRTGAHIREAFSLFDEKIKGSVVSACPMEHHPLKTLLQINNGEYAPMRHLSD
                     LEQPRQQLPQAFRPNGAIYINDTASLIANNCFFIAPTKLYIMSHQDSIDIDTELDLQQ
                     AENILNHKES"
     gene            10219..11268
                     /gene="siaC"
                     /locus_tag="ACFM8S_000722"
     CDS             10219..11268
                     /gene="siaC"
                     /locus_tag="ACFM8S_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialic acid capsule biosynthesis protein
                     SiaC"
                     /protein_id="HFC6313826.1"
                     /translation="MQNNNEFKIGNRSVGYNHEPLIICEIGINHEGSLKTAFEMVDAA
                     YNAGAEVVKHQTHIVEDEMSDEAKQVIPGNADVSIYEIMERCALNEEDEIKLKEYVES
                     KGMIFISTPFSRAAALRLQRMDIPAYKIGSGECNNYPLIKLVASFGKPIILSTGMNSI
                     ESIKKSVEIIREAGVPYALLHCTNIYPTPYEDVRLGGMNDLSEAFPDAIIGLSDHTLD
                     NYACLGAVALGGSILERHFTDRMDRPGPDIVCSMNPDTFKELKQGAHALKLARGGKKD
                     TIIAGEKPTKDFAFASVVADKDIKKGELLSGDNLWVKRPGNGDFSVNEYETLFGKVAA
                     CNIRKGAQIKKTDIE"
     gene            11352..12839
                     /locus_tag="ACFM8S_000723"
     CDS             11352..12839
                     /locus_tag="ACFM8S_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2,8-polysialyltransferase family protein"
                     /protein_id="HFC6313827.1"
                     /translation="MLKKIKKALFQPKKFFQDSMWLTTSPFYLTPPRNNLFVISNLGQ
                     LNQVQSLIKIQKLTNNLLVILYTSKNLKMPKLVHQSANKNLFESIYLFELPRSPNNIT
                     PKKLLYIYRSYKKILNIIQPAHLYMLSFTGHYSYLISIAKKKNITTHLIDEGTGTYAP
                     LLESFSYHPTKLERYLIGNNLNIKGYIDHFDILHVPFPEYAKKIFNAKKYNRFFAHAG
                     GISINNNIANLQKKYQISKNDYIFVSQRYPISDDLYYKSIVEILNSISLQIKGKIFIK
                     LHPKEMGNNYVMSLFLNMVEINPRLVVINEPPFLIEPLIYLTNPKGIIGLASSSLIYT
                     PLLSPSTQCLSIGELIINLIQKYSMVENTEMIQEHLEIIKKFNFINILNDLNGVISNP
                     LFKTEETFETLLKSAEFAYKSKNYFQAIFYWQLASKNNITLLGHKALWYYNALYNVKQ
                     IYKMEYSDIFYIDNISVDFHSKDKLTWEKIKHYYYSADNRIGRDR"
     gene            complement(13335..14492)
                     /locus_tag="ACFM8S_000724"
     CDS             complement(13335..14492)
                     /locus_tag="ACFM8S_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6716 putative glycosyltransferase"
                     /protein_id="HFC6313828.1"
                     /translation="MKKVLLIASYDSFLNSGYAVAKEIKDAQIDIYIHKSRENILSNR
                     QLLESGIDKDQAIFFHIDDYFIKNMHQYYDAVILSVGNGLLKRFFKQNAQLNIASRPL
                     IITLFPGVVFGDQASILSRMGADIVLYNNKHDFRIAEEYKKQYKLSCQNILYGYPIFR
                     HASKGRHGEKIYFIDQVKIPFKKEERIYTLKKLIALAEKYPEKEFTILLRVADKDITV
                     HQDKHSYIELAKQFQLPSNLTIERKSTAQAFQEMGYCLSYSSTMLFEAECKGIPVGVV
                     ADLGFSKSYANQHFLGSGVLVYFDQIDFTSPKIADPDWLDCYATKKVITTDEFNKLLK
                     QVVPLQHDYQEYLSAVNSIESTKTIFLRKFKKLIRDLKKFFYDSKWLRKVI"
     gene            14669..15688
                     /gene="galE"
                     /locus_tag="ACFM8S_000725"
     CDS             14669..15688
                     /gene="galE"
                     /locus_tag="ACFM8S_000725"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFC6313829.1"
                     /translation="MKKILVTGGTGFIGSHTVVSLLKSGHQVVILDNLCNSSINILPR
                     LKTITGQEIPFYQGDIRDREILRRIFAENRIDSVIHFAGLKAVGESVAEPMKYYDNNV
                     SGSLVLAEEMARAGVFSIVFSSSATVYGDPGKVPYTEDMPLGDTTSPYGTSKSMVERI
                     LTDIQKADPRWSVILLRYFNPIGAHESGLIGEQPNGIPNNLLPYICQVAAGKLPQLAV
                     FGDDYPTPDGTGMRDYIHVMDLAEGHVAAMKAKGGVAGVHLFNLGSGRAYSVLEIIRA
                     FEAASGLTIPFEIKPRRAGDLACFYADPSYTKAQIGWQTQRDLSQMMEDSWRWVSNHP
                     NGYDD"
BASE COUNT         4597 a         3633 c         3184 g         4430 t
ORIGIN      
        1 ccgataaagc ccgcaccgcc ggtaacgagg atggtttttt tgtttgcggt ttgcatttaa
       61 tccccatatc cgttggggtt gcggctgacc caacgccacg aatcttccat catttgctgc
      121 aagccgcgtt tggtttccca gccggtttgt tgtttggtat gggacgggtc ggcaaaggaa
      181 cacgccaaat caccggcgcg gcgcggtttg atttcaaatg gaatggttaa acccgaagcc
      241 gcttcaaatg cgcggatgat ttccaatacc gaagaagcgc ggccggagcc taagttcagc
      301 aaatgcgtgc ctgctacatt actttttgcc tgcatagccg cgacatggcc ttctgccaaa
      361 tccatcacat gaatatagtc acgcatcccc gtgccgtcgg gggtagggta gtcatcgcca
      421 aataccgcca attgcggcag tttgcctgcc gccacttggc agatataagg caacaaatta
      481 ttcgggatgc cgtttggctg ctcgccaatc aaaccacttt cgtgcgcccc aatcgggttg
      541 aaatagcgca acaaaatcac gctccaacgc ggatcggcct tttggatgtc ggttaacatc
      601 cgttccacca tcgctttgga tgcgccataa ggattagcgg tatcgcccgg gcgcatatct
      661 tccgtatagg gcattttacg tatcatggcg cgacgcatta aagtaaacgc caaagcgcgt
      721 ttatactggt tatccagttt ccctatattt ttacgttaca aatcaagttg ttggtattca
      781 tcgtgaaaat agaatttttc ggcataacgc tgactcataa aaccggcatg cgggctgcct
      841 gaaaaaatca ttgcgccatc atcgtcattt aatgtttcat ggttgttttc tattaatgct
      901 aaagcaattt ggtaattaat tgccaaaata tcgttagtaa tcacacgata gccgcgtttt
      961 ttggcttcat aggcaaagga acagcctcca ctaaatacat ctgcaactgt atctacatca
     1021 gacggaagct ggtcacaaat ccaggaagct attttctctt tattaccgat atagttaatt
     1081 ttcggatatt gcttattcat tttcactcat ttttaaaata ctttcagcaa tagcttttgc
     1141 caataaaggc ggtacggcgt taccaacctg ctgctgctgg gcaattttgc tgccgcaaaa
     1201 aataaaatta tcagggaaag attgtaaggc agctaattca cgaacggtta acgcccgatt
     1261 ctgttcatag tgaaaaactt tgcgcatatc tcctgtaata caaacggctg gttttgttgc
     1321 tgttgtaacg gatgtattta cggatatcac ctgttttcgg acgtaatggt tcaggaatat
     1381 cgttacggtt acctccattt ttaacaaatg ccattttttc taacatttgt gccgaatgat
     1441 tcatagcttc atgatttgca acgtgtggat tgctttcgcc agcagccagt tttggaaaat
     1501 gtcctattgc tgatccaaca gtctgatggg aaatctgcaa aggttcggga aaggaaattt
     1561 tgcctttatc cctcctcccg ataaatatca ctcggctacg tatctgagga acaccgaaat
     1621 cggctgcact cagtatctta cattccaccg aataaccgat attctgaaat gcttgaataa
     1681 tctcaatacg tgttttacct gaattgtgtg tatagagtcg cgctacattt tccataacaa
     1741 aaaaatatgg ttggacaatt ttaactattc ggacaaactc tttaaataaa tggttgcgtg
     1801 ggtcatctgt aaatgtccgt ccaatctttc ctgccatact aaaaccttga caaggtggtc
     1861 ctccaataat caaatcaact gcttgtccgt taagacaatt gattaaatct tgttcggtta
     1921 gtgtggttaa atctttttgc agtaattgat gatgggggaa gttggtacgg taagtctgac
     1981 aataatcaga ctccatttca acagaaagca attgttggaa tccggcttgt tcaaaaccca
     2041 aggataggcc tcctgctcct gagaaaaggt caatataggt aagtggtgtg tgcataagtc
     2101 aaaatccata aactcttcga ttattttacc gcttcagctt cgcaaacgca tccgccatcg
     2161 ccgaattggc tggggcgcgg tcgttgcgtt ggggtttgcg gccggctgtc cgttcgcggc
     2221 tgcggttttc agacggcatt ttatgttttg cgccgcccgg ttcgtcatcc aagcgcatgg
     2281 tcagcgcgat gcgtttgcgt gcagcatcga cttccagcac tttcactttc accacgtcgc
     2341 cagctttcac cacttcgcgc gggtcttgga cgaacttgtt ggacagggcg gagatgtgca
     2401 ccaagccgtc ctgatggacg ccgatgtcca cgaacgcgcc gaagttggcg acgttggaaa
     2461 ccacgccttc gagtatcata ccgacttgca agtcgctgat ttcgtggata ccttcggcaa
     2521 acgatgccgt ctgaaactcg ccgcgcggat cacggccggg tttttccagt tcggacagga
     2581 tgtccaaaat ggtcggcagg ccgaagcgtt cgtcggtgaa gtcggacgct ttgatttgct
     2641 tcacgcgctc gcggttgccg atgagttcgg cggcgctaat gccttgttgc gccagcattt
     2701 tggcgacgac gggataggct tcggggtgga cggcgctcgc gtccaacggc tctttaccgc
     2761 cgttaatccg caaaaagcct gccgcctgct cgaaggtttt ttcgcccaaa cgcggtactt
     2821 tcagcaattt tttgcggctg tcgaacgcgc cgttttcatc gcggtaggca acgatgtttt
     2881 gggcaagggt ttgattcaaa ccggaaattc gcgccaagag cggggcggag gcggtgttca
     2941 cgtccacgcc gacggcgttc acgcagtctt cgaccaccgc gtccagcgat ttggcgagct
     3001 ggctttggtt cacgtcgtgc tgatactgcc ccacgccgat ggatttgggg tcgattttga
     3061 ccaactcggc aagcgggtct tgtagcctgc gggcgatgga caccgcgccg cgcagggaaa
     3121 cgtccaagtc ggggaactcg cgcgccgcca gttcggacgc ggaataaatc gacgcgccgg
     3181 cttcggacac gacgatttta tgcagcccca ttttcgacat ttcacgcacc agttcgcccg
     3241 cgattttgtc ggtttcacgg ctggcggtgc cgttaccgat ggcgatgagc ttcacgccgt
     3301 gctgtttaat caggcgcgac agcgttgcca acatattgtt ttcttgatgc aaatagacga
     3361 tgacggtatc cagcagcttg ccggtgtcgt ccaccacggc gcatttcacg ccgttgcggt
     3421 agccggggtc gagacccagc gtggtcagcc gtccggcggg cgcagcaagc agcaagtctt
     3481 tgagattgcg ggcgaacacg gtaatcgcgt cggtgtcggc ggcttctttc aaacgattga
     3541 gagcttcaag ttccaacgac aaaaagattt tcgcgcgcca agtcaggcgc acggtgtcgc
     3601 gcagccattt gtggccgtct gaaactttga agcggcgggc gatgatttgc tcgtattcgc
     3661 tttgctgcgt aatcggcgtg tcgtcgggct gatatttgag cgcgatgttc aacacgcctt
     3721 cgttgcgtcc gcgcaaaacc gctagcgcac gatggctggg catcgcacga ataggttcgc
     3781 ggtggtcaaa ataatcgctg aatttttcgc cttcggtttc tttgcctcca acaacttgcg
     3841 cgtggatttc ggcttcgttc cacagcttgt cgcgcagcgt gccgatgagt tccgcgtctt
     3901 cggcaaactg ctccatcaga atcgcacgcg cgccgtccaa cgcggctttg gcatcgggga
     3961 cgttttcgtt caggtagcct tgtgcggcgg cttccacgtc ctgcgactgc tcggcaagca
     4021 acacgtccgc cagcggctgc aaaccgtgtt cgcgcgcaat ttgcgccttg gtgcggcgtt
     4081 tgggcttgta gggcagatac aggtcttcca gcgcggtttt gttgtcggcg gcttcgattt
     4141 gcgcgcggag gccgtctgaa agtttgcctt gctcttcaat gctttttaac acaacggctt
     4201 tgcgctcttc caactcgcgc aggtattgca gccgctcggc aagccggcgc aactgcgtat
     4261 cgtccaaccc gcccgtcgct tccttgcggt agcgggcgat aaacggcacg gtcgcgccgt
     4321 cgtccaaaag ctcgacggcg gcggtgattt gcgcggcagt cgcggagagt tcttgggaaa
     4381 gaatttgagt aatgttcatc aatagaattc caacggacag gccgtctgaa atttcagacg
     4441 gcctgattta aaaacaatcg ctttaaggca gcgaattata atattcgtag gctttgtcca
     4501 tatcttcaaa ctggtacata tgcccttttt ccagcaccat cgcattatcg caatattgct
     4561 tcatggcgct gtggctgtgc gacaccaaga tgatggaacg gtctttgcgc ttttcaaaca
     4621 actcgtactt acatttatcg gcaaaacgcg agtcaccaac tgcaatcact tcgtcaatca
     4681 aatagcaatc aaactctacc gccaacgaca aagcaaatgc caaacgcgcc ttcatacctg
     4741 aagaataacg cttgaccggc tcgtaaagat attgccccaa ctcggaaaac tcttcggtaa
     4801 agttcttcac ataatcgata tcgacattgt aaatccggca gatgaaacgc aaattgtcca
     4861 taccggtcag actaccttga aacgcaccgg agaatgccaa aggccaagaa atactcattg
     4921 tccgcttgat ttcacccgtg gtcggcggct caacgccact gatcaaacgg atgagcgtcg
     4981 atttacctgc accgttgcgg ccgagaatac cgattttctc gcccttctcc attttgaagc
     5041 taatatcgtg caagactgtc cgccaacctt ggcgggtcag atagcgtttg gaaacgtgtt
     5101 caactgaaat cattgcggct caaccccttt gctgaatctc gccaccaccg ccaaaccaag
     5161 cagcagcaaa accaagttac acaacaaaat ataccaagga ttttcgtaag tcgtgacgct
     5221 gtcgccaaaa tatccggcac ggaacatttc tgtgccatgc accatcggga tcatcaatgc
     5281 atactcttgc actttcggag gcaaattatg tacaaaaaag aatgcacccg ataacggcat
     5341 catcacaaaa gtcaatgtgc cccaaatctt gccaaacggc tcgaaattaa aggcaatcga
     5401 acaaatcacc aaacccaaac caatcgcaaa aaaagccatc aaaagccaag ccatcagcat
     5461 ataaaacata tctgccggca tttcaatcca gccaatcgca atcaataccg ccataatcac
     5521 aatctgcgca atggttgcac cagcaatttc caaaatcatg cgcgccaaga tggtatccaa
     5581 aactcttaca ttgcggtgat aaagcaagct ggcatttgaa gaaatcgacc caactgcccg
     5641 ttttgaggca ttacgccaca tcatcaacat cggatagcca gtaatcgcaa atgcgacaat
     5701 attcaaagtt gaatatcggt ctgcctttaa aaatttccac atcaagacga taacgaatgt
     5761 catcagcaac ggctcaacaa acagccataa aaagccaata ttattgcgac cgtaacgggt
     5821 gataatttcc cgcatcaaaa gcgcaccgat tacgcgcctt tgaatggcta aagattccca
     5881 aaatgatgtt ttatgcaagg ctttcatcag tttttatgct cacgaatgct ggcagtcaac
     5941 aggctcaaaa taccataaac catcaagccg ataatcagag tggcaacaat gttgtataac
     6001 cgtttaggct catgtgccaa atccggcagg ctcggttgcg agatcacttc caaataaagc
     6061 tgctgacggt ctgcttcaac cttggcactt tccaaagaag tcatggcggc tgccaactgc
     6121 tgctctgcca actggttttc caaatacaca cgctgatatt cggcagcctg attagacaac
     6181 gaagaatgcc caccgcccga aatggcacgt aactgttggt caatttcttt acgcaagctc
     6241 tgctcacgcg cctgcaaacc cggaatctgc ggattctccg gagtgactgc tttcacctga
     6301 tccagctggg tttgaatcac aatcaattca tcttgcagct tggaaaccaa ccccatttgc
     6361 acttccgatt gcgctttcaa atcaaaaacg ccattggcaa tccggtaatc cgtcagattc
     6421 tgagaggctt cctttacccg ctctgccgcc gttttcacta cttcttccgc atagcgcacc
     6481 gtatcagcac gtgcacgatc gttcaactgg ttaatcaatg cttcaccttg ttttaacaaa
     6541 gcctcattga ttttcttaga ttccagcgca tcaaaggaag ttacattcaa cgtggaaata
     6601 cccgaaaccg tatcaaaatt gatcatcacc tgatttttat agtattgata aaaagcctct
     6661 tcctcgccac ggaatccaaa accattaaag cggctgaacg catcaccttt ggtttcataa
     6721 aactcacgca ccggcaagat tttacgcagt tcatccaaag acgagcgcga acgcatatac
     6781 tccccaaccg tgtaaatatc atcttgcgca cgggcaaaac ctgtgccctg caaaatggca
     6841 cccaggccat tgagagaaga ttggctttta ggcgagcgca ccacaaagct cgattgcgac
     6901 gtaaaacgat cggaagcgaa gaagccgaaa tacaccaacg aaattaccgt agggataatc
     6961 accgttaccc aaaataaagg gcttagcttt ttaatccaac ttttcttttt cggcttttta
     7021 cgctcggctt tggtttcagt ggcaactgcc acaggaagtt gctcagacat cttacttact
     7081 cacattaatt agttaaatta ttaatactgt tcgcgccact ggtaaccggc gagaacacaa
     7141 acgacaaaaa tttctgcact tcagccaacg gcgcattcga cacatacaat acatctttat
     7201 tcttcacagg aaagcgctgc atagaaaata gcgaatgcgc atcagccata ttcacacgat
     7261 ataccgttgg aatctctgcc tcactgccat aaccttgagc aatccattta tcctgacgtt
     7321 ctgccggcaa ttccaccaat ggcgtatagc ggaacacaaa cacaccacgc gcatcagaac
     7381 ggcgatcttg caaaccgccc atacggccaa tggcttcaga aagcgataag cctctggctg
     7441 aaaaaccgat ttcttgtgtt ctccccaccg cacccataga cgtaaaggta tagggattgg
     7501 taatcatggt aaccacatca ccgcgacgca gcaaaatatt ttgtcgcgga tttgcaacta
     7561 aatcttccaa ggcaacagtt cgtactacat tgccacgtgt cagctgcaca ttcgtatcct
     7621 gcacatttgc cgttgaacca cctaccgcag ccaccgcatc caacacacgc tcaccggctg
     7681 ccgtcagcgg catacgcaca ctattcccag cacgaatcac cgacacattc gccgcattat
     7741 tctgcaccaa acgcaccatc acttgtggct gattggccat ttttttcagg cggcctttaa
     7801 taatttcctg aacctgacca ggcgttttac cgaccaccga aatatcgcca acaaacggca
     7861 cagaaaccgt accacgtgcc gtgaccaact gctctggcaa cttagtttga tgcgcactac
     7921 ccgagcccat cgaagaaagg ccaccaccaa acaatactgc cggcggcgct tcccaaatca
     7981 taatatccaa tacatcacca atatttagcg taccagccga agcataacca tcgccaaact
     8041 gagtgaatga ctgatttatc tgagccttat ataataactg agcaaccgta tgattcacat
     8101 caatcagctc cacttcagga atttgaactt cagattgttg ccctaaagag acaatttttt
     8161 ttgcgctggg gcctgatgaa ggaatcgcag agcatcctac aattaaactt ccacacaata
     8221 ataatactgc gtgacgaata taaaatttca ctttaaacac aagccaaatc ctaatataat
     8281 tataaatggc ctaattatag cacttaatcg aaataaattt atgagtacgt agagtataat
     8341 tagtattctt ctttccaact tccttatact tatatactta tagattctaa aatcatgaaa
     8401 agaattcttt gcattacagg taccagagcc gacttcggca agctaaaacc tttattagcc
     8461 tatattgaaa atcacccaga ccttgaattg catttgattg taactggtat gcatatgatg
     8521 aaaacatatg gcagaacctg caaggaagta actcgagaaa actatcaaca tacatatctg
     8581 ttttcaaatc aaatccaagg tgaaccaatg ggtgccgttt taggcaatac cattacgttt
     8641 atctctcgtc tatctgatga aattgaacct gatatggtca tgattcacgg cgaccgttta
     8701 gaagcactag caggcgcaac tgtaggtgca ttaagcagcc gtttagtttg ccatatcgaa
     8761 ggtggtgaac tatctggtac agtagatgac tccattcgtc attctattag taaactttct
     8821 catatccact tggtagcaaa tgaacaagct gtcactcgcc tagtgcaaat gggagaaaaa
     8881 agaaagcata ttcacatcat cggctccccc gatttagatg ttatggcctc ttccaccctc
     8941 ccatccttag aagaagtcaa agaatattac ggtttaccat acgaaaatta tggtatttct
     9001 atgtttcacc ccgtgactac agaagcacat ttaatgccac aatatgcggc ccaatatttc
     9061 aaagcattag aattaagtgg ccaaaatatc attagcattt accctaacaa cgacacgggt
     9121 actgaaagta ttttacaaga gttattaaaa tgtcaatctg aaaaatttat cgcatttcca
     9181 tctatacgat ttgaatactt tttagtctta ttgaaacatg ctaaatttat ggtcggcaac
     9241 tcaagtgcag gtattagaga agctcctctc tacggtgtcc cttcaattga tgttggtaca
     9301 cgccaaagta accgccatat gggaaaatct attattcata cagattatga aactaaaaat
     9361 atctttgatg cgattcaaca agcatgcagt ttaggcaaat ttgaagcaga tgataccttt
     9421 aatggcggag atactcgcac cagcacagaa agatttgctg aagtaatcaa caatcctgaa
     9481 acgtggaatg tttctgctca aaaacgtttt atcgatttga atctttaaat tatggaaaaa
     9541 caaaatattg cggttatact tgcgcgccaa aactccaaag gattgccatt aaaaaatctc
     9601 cggaaaatga atggcatatc attacttggt catacaatta atgctgctat atcatcaaag
     9661 tgttttgacc gcataattgt ttcgactgat ggcgggttaa ttgcagaaga agctaaaaat
     9721 ttcggtgtcg aagtcgtcct acgccctgca gagctggcct ccgatacagc cagctctatt
     9781 tcaggtgtaa tacatgcttt agaaacaatt ggcagtaatt ccggcacagt aaccctatta
     9841 caaccaacca gtccattacg cacaggggct catattcgtg aagctttttc tctatttgat
     9901 gagaaaataa aaggatccgt tgtctctgca tgcccaatgg agcatcatcc actaaaaacc
     9961 ctgcttcaaa tcaataatgg cgaatatgcc cccatgcgcc atctaagcga tttggagcag
    10021 cctcgccaac aattacctca ggcatttagg cctaatggtg caatttacat taatgatact
    10081 gcttcactaa ttgcaaataa ttgttttttt atcgccccaa ccaaacttta tattatgtct
    10141 catcaagact ctatcgatat tgatactgag cttgatttac aacaggcaga aaacattctt
    10201 aatcacaagg aaagctaaat gcaaaacaac aacgaattta aaattggtaa tcgttcagta
    10261 ggttacaacc acgaaccatt gattatctgt gaaatcggca tcaatcatga aggctcttta
    10321 aaaacagctt ttgaaatggt tgatgctgcc tataatgcag gcgctgaagt tgttaaacat
    10381 caaacacaca tcgttgaaga cgaaatgtct gatgaggcca aacaagtcat tccaggcaat
    10441 gcagatgtct ctatttatga aattatggaa cgttgcgccc tgaatgaaga agatgagatt
    10501 aaattaaaag aatacgtaga gagtaagggt atgattttta tcagtactcc tttctctcgt
    10561 gcagctgctt tacgattaca acgtatggat attccagcat ataaaatcgg ctctggcgaa
    10621 tgtaataact acccattaat taaactggtg gcctcttttg gtaagcctat tattctctct
    10681 accggcatga attctattga aagcatcaaa aagtcggtag aaattattcg agaagcaggg
    10741 gtaccttatg ctttgcttca ctgtaccaac atctacccaa ccccttacga agatgttcga
    10801 ttgggtggta tgaacgattt atctgaagcc tttccagacg caatcattgg cctgtctgac
    10861 cataccttag ataactatgc ttgcttagga gcagtagctt taggcggttc gattttagag
    10921 cgtcacttta ctgaccgcat ggatcgccca ggtccggata ttgtatgctc tatgaatccg
    10981 gatactttta aagagctcaa gcaaggcgct catgctttaa aattggcacg cggcggcaaa
    11041 aaagacacga ttatcgcggg agaaaagcca actaaagatt tcgcctttgc atctgtcgta
    11101 gcagataaag acattaaaaa aggagaactg ttgtccggag ataacctatg ggttaaacgc
    11161 ccaggcaatg gagacttcag cgtcaacgaa tatgaaacat tatttggtaa ggtcgctgct
    11221 tgcaatattc gcaaaggtgc tcaaatcaaa aaaactgata ttgaataatg cttattaact
    11281 tagttacttt attaacagag gattggctat tacatatagc taattctcat taatttttaa
    11341 gagatacaat aatgctaaag aaaataaaaa aagctctttt tcagcctaaa aagttttttc
    11401 aagattcaat gtggttgaca acatctccat tttatcttac ccccccacgt aacaatttat
    11461 ttgtcatatc taatttaggt cagcttaacc aagtccaaag cctaattaaa atacaaaaat
    11521 taaccaataa tttactagta attttatata cttctaaaaa cttaaaaatg cctaagttag
    11581 ttcatcaatc agctaacaag aatctatttg aatctattta tctatttgag cttcctagaa
    11641 gccctaataa tataactcct aaaaaattac tttatattta tagaagttac aaaaaaatcc
    11701 ttaatattat acagcctgct catctctata tgctgtcttt tacaggccac tactcctatc
    11761 tgattagtat tgcaaaaaaa aagaatatta cgactcattt aattgatgaa gggactggaa
    11821 catatgctcc tttattagaa tcattttcat atcatccaac aaaattagaa cgttatttga
    11881 ttggaaataa tcttaatatt aaaggatata tagatcattt tgacatattg catgtcccct
    11941 ttcctgaata tgctaaaaaa atatttaatg caaaaaaata taaccggttt tttgcgcatg
    12001 ctggaggaat aagcattaat aataacattg caaacttaca gaaaaaatat caaatatcta
    12061 aaaatgacta tatttttgtt agtcaacgct accccatttc agatgatttg tattataaga
    12121 gtatagtaga aatcttaaac agcataagtt tacaaattaa aggaaagata tttattaaac
    12181 tacacccaaa agagatgggc aacaactatg taatgtcttt atttctaaat atggtagaaa
    12241 taaaccctcg gctggtagtt attaatgaac ctccttttct aattgagccc ctaatatact
    12301 taacaaatcc taaaggaatt ataggcctgg cctctagttc tttaatttat acaccattac
    12361 tctcaccctc aacccaatgt ctttctattg gagagttaat tattaactta attcaaaaat
    12421 attcaatggt ggaaaacact gaaatgatcc aagaacactt agagattatt aagaaattta
    12481 attttattaa tatactaaat gatttaaatg gggtaataag taaccctctc tttaaaacag
    12541 aagaaacatt tgaaacactt cttaaatctg cagaattcgc atataaatct aaaaactact
    12601 ttcaggctat tttttactgg caacttgcca gcaaaaacaa tattacctta ttagggcata
    12661 aagcattatg gtactacaat gcactttata atgtaaaaca aatttataag atggaatatt
    12721 cagatatttt ttatatcgat aatatctccg tagactttca tagtaaagat aaattgacat
    12781 gggaaaaaat taaacattat tactattccg ccgacaatag aattggtaga gatagataaa
    12841 aatactatga cttctgatct ccatacgtat gcttgttttc aaaaagcagc attaagatag
    12901 ataagtgaag cttgatgaga gccatttttg tggtcatcgc caaggtacac gcggagagta
    12961 atcgacaaca gagctgctgt attctggctt ttaaagccca gtagtaaagt ttataaggct
    13021 gcatttctca atgtaaaacg cgatattttc acccattatt agggataagt agccagtggt
    13081 ttacactggc tactttcata gttatgaagt actgggtctg agtgaattca attatttccg
    13141 tataaaacac agtagacatt ttactaatcg gcaaaattat attagaagaa ttgagaacta
    13201 ctgaagtcag gtaagatacc attttgcaca aatttaacgg catccctaaa aagcattttg
    13261 ggtggtattt aaaggagtac aaaatggttt taactacagt atgcttagaa atctatagcc
    13321 ttacgcgctt tatattaaat cacctttctc aaccatttag aatcataaaa aaacttttta
    13381 agatccctaa ttaacttttt aaattttctt aaaaaaatag ttttagttga ttctatacta
    13441 ttcaccgcag ataaatattc ttggtaatca tgttgcaatg gcacaacctg ctttaatagc
    13501 ttattaaact catcagttgt aatcaccttt ttagtagcat agcaatcaag ccaatccgga
    13561 tctgctattt ttggggaagt gaaatctatt tgatcaaaat aaactaaaac cccactacct
    13621 aaaaaatgct gatttgcata ggatttagaa aagcctaagt ctgcaacaac accaacaggg
    13681 atacccttac attcagcttc aaaaagcata gtagatgaat aagataaaca ataccccatt
    13741 tcttggaagg cttgcgcggt actttttcgc tctattgtca aattactcgg caactgaaac
    13801 tgctttgcca gctctatata cgaatgttta tcctgatgca cagtaatatc tttatctgca
    13861 acccttagca aaatagtaaa ttctttctca gggtattttt cagccaaggc aatcaatttt
    13921 tttaatgtat aaattctttc ttctttttta aatgggattt taacttggtc aataaagtaa
    13981 attttctctc catgacgacc ttttgatgcg tggcgaaaaa ttggataacc ataaagtata
    14041 ttttgacaac ttaatttata ttgtttctta tattcctctg caattctaaa atcatgctta
    14101 ttattatata aaacaatatc agcccccata cgagatagaa tacttgcctg atcaccgaat
    14161 actacacctg gaaacaaggt aataatcaat ggccttgaag caatatttaa ttgcgcattc
    14221 tgcttaaaga acctttttaa caacccattt ccaaccgata aaattactgc gtcataatat
    14281 tgatgcatat tcttaataaa gtaatcatca atatgaaaaa aaattgcttg gtctttatct
    14341 atccctgatt ctaataactg tcgatttgaa agaatgtttt ctcgactttt gtggatataa
    14401 atatcaattt gagcatcttt tatctctttt gcaacagcat agcccgagtt aaggaacgag
    14461 tcataactgg caattagtaa tacttttttc actattttcc cttaattcta catcatatga
    14521 ataaaattat agtattttac cttagatatc aaatatactt aaccaaaaaa atctgaagaa
    14581 atctactgct acttcaggga taattatttc attaagattt tacaaccatt attatcaatc
    14641 tatcaaagta tttttagtat tttattctat gaaaaaaatt ctcgttaccg gcggcaccgg
    14701 ttttatcggc tcgcataccg ttgtttcttt gctgaaaagc ggccatcaag tcgtgatttt
    14761 ggataaccta tgcaattcca gcatcaatat cctgccacgc ttgaaaacga ttaccggcca
    14821 agagattccg ttttatcaag gcgatatccg cgatcgtgag attttgcgcc gtatttttgc
    14881 ggaaaaccgc attgattcgg tgattcattt tgccggcttg aaagcggtgg gtgaaagtgt
    14941 ggccgagccg atgaaatatt atgataataa tgtttccggc agcttggtgt tggcggaaga
    15001 aatggcgcgt gcgggcgtgt ttagcattgt gttcagttct tcggcgacgg tttatggcga
    15061 tccgggcaaa gtgccttata ccgaggatat gccactgggc gataccacca gcccttacgg
    15121 cacatcgaaa tcaatggttg agcgcattct cactgatatt caaaaagccg atccgcgctg
    15181 gagcgtgatt ttgttgcgtt atttcaatcc gattggcgcg cacgaaagtg gtttgattgg
    15241 cgagcagcca aacggcatcc cgaataattt gttgccttat atctgccaag tggcggcagg
    15301 caaactgccg caattggcgg tatttggcga tgactaccct acccccgacg gcacggggat
    15361 gcgtgactat atccatgtaa tggatttggc ggaaggccat gtcgcggcaa tgaaggcaaa
    15421 aggcggcgtt gccggcgtac atttgttcaa cttgggttcg gggcgcgcct attccgtttt
    15481 ggaaatcatc cgcgcatttg aagcggcttc gggtttaacc attccatttg aaatcaaacc
    15541 acgccgtgcg ggcgatttgg cgtgtttcta tgctgaccct tcttatacga aggcacaaat
    15601 cggttggcaa actcagcgtg atttaagcca aatgatggaa gattcatggc gctgggtaag
    15661 taatcatccg aatggctacg acgattaagt tgacctgata caggccgtct gaaagagatg
    15721 ttttcagacg gcctctttat ctgaaaaaca cacattctgt ctgctataat ctgtttatat
    15781 tttttggcta tcctctgaaa tttatgagaa aaatccttgt taccggcggc gcgggcttta
    15841 tcgg
//
LOCUS       DAXHSF010000029        15127 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.029, whole genome shotgun sequence.
ACCESSION   DAXHSF010000029 DAXHSF010000000
VERSION     DAXHSF010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15127
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.029"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(87..860)
                     /locus_tag="ACFM8S_000726"
     CDS             complement(87..860)
                     /locus_tag="ACFM8S_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFC6313830.1"
                     /translation="MSANILAIANQKGGVGKTTTTVNLAASLASRGKRVLVVDLDPQG
                     NATTGSGIDKAGLQSGVYQVLLGDADVKSAAVRGKEGGYAVLGANRALAGAEIELVQE
                     IAREVRLKNALKAVAEDYDFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGIS
                     DLIATVRKIRQAVNPDLDITGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETVIPRNI
                     RLAEAPSHGMPVMAYDAQAKGTKAYLALADELAARVSGK"
     gene            complement(959..1582)
                     /gene="rsmG"
                     /locus_tag="ACFM8S_000727"
     CDS             complement(959..1582)
                     /gene="rsmG"
                     /locus_tag="ACFM8S_000727"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFC6313831.1"
                     /translation="MERKERLQSGIAAMGLDISETAQDRLLAYVDLLKKWNKTYNLTA
                     LRDEEKMIVHHLLDSLTLLPHIEGVQTMLDVGSGGGQPGIPAAVCRPDVQITLLDANT
                     KKTAFLQQAVIELGLDNVRVVSGRVEAVSDVRADVVTSRAFAELADFVSWTVHLLKDG
                     GYWAAMKGVYPQEEIGRLPQDVCVEKVQRLDVPGLDAERHIVILSKR"
     gene            complement(1678..2163)
                     /locus_tag="ACFM8S_000728"
     CDS             complement(1678..2163)
                     /locus_tag="ACFM8S_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313832.1"
                     /translation="MYVNEKYPYAALFAGLVFLTLPFALSVHDALAFAFGRTGLLVSV
                     SDGGFGWRGGWDSTVWFVFGVFAFLNVVVSAGLTKLAYKKMMRRHSRYALFLSGVAAC
                     AAAAVAWIFELLLGSAALGGLRGEAVLEYAFAVWLVAMLTLPKRLTRAPVQLVVFHRK
                     K"
     gene            2269..2344
                     /locus_tag="ACFM8S_000729"
     tRNA            2269..2344
                     /locus_tag="ACFM8S_000729"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2302..2304,aa:Thr,seq:cgt)
     gene            2420..3547
                     /locus_tag="ACFM8S_000730"
     CDS             2420..3547
                     /locus_tag="ACFM8S_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFC6313833.1"
                     /translation="MNTSQRNRLVSRWLNSYERYRYRRLIHAVRLGGAVLFATASARL
                     LHLQHGEWIGMTVFVVLGMLQFQGAIYSKAVERMLGTVIGLGAGLGVLWLNQHYFHGN
                     LLFYLTVGTASALAGWAAVGKNGYVPMLAGLTMCMLIGDNGSEWFDSGLMRAMNVLIG
                     AAIAIAAAKLLPLKSTLMWRFMLADNLTDCSKMIAEISNGRRMTRERLEENMAKMRQI
                     NARMVKSRSHLAATSGESRISPAMMEAMQHAHRKIVNTTELLLTTAAKLQSPKLNGSE
                     IRLLDRHFTLLQTDLQQTVALINGRHARRIRIDTAINPELEALAEHLHYQWQGFLWLS
                     TNMRQEISALVILLQRTRRKWLNAHERQHLRQSLLETREHG"
     gene            3728..4285
                     /gene="frr"
                     /locus_tag="ACFM8S_000731"
     CDS             3728..4285
                     /gene="frr"
                     /locus_tag="ACFM8S_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFC6313834.1"
                     /translation="MINDIQKTAEGKMQRSVEVLKENLAKVRTGRAHTGLLDQVEVEY
                     WGSMVPVSQVANVTLLDARTIGVKPFEGNMAAKVEKAIRDSNLGLNPAAVGDLIRVPM
                     PMLTEERRKDLIKVVRGEAEEGRVSIRNVRRDANDHIKKLLKDKEISEDEARRGEEAV
                     QKLTDKYITEADKLLTAKEEDLMAI"
     gene            4341..5087
                     /locus_tag="ACFM8S_000732"
     CDS             4341..5087
                     /locus_tag="ACFM8S_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFC6313835.1"
                     /translation="MKSSTQAVLEHTAIPKHIAVIMDGNGRWAKKRFLPRIMGHKRGL
                     DALENMVKHCAKLGVQYLTVFAFSTENWRRPEDEVSFLMGLFLQALQKQVRRLHENNM
                     RLKILGSRERFNRQILQGIEEAEALTANNTGLTLSIAADYGGRWDILQAANKLIAEGV
                     SEITEDTLAKHLMLGDAPEPDLFIRTGGETRISNFLLWQMAYAELYFTDILWPDFDGK
                     ALDDAVASFQKRERRFGRTSEQLPIEQQRN"
     gene            5090..5887
                     /locus_tag="ACFM8S_000733"
     CDS             5090..5887
                     /locus_tag="ACFM8S_000733"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFC6313836.1"
                     /translation="MLKQRVITAMWLLPLMLGMLFYAPQWLWAAFCGLIALIALWEYA
                     RMGGLCKIKTNHYLVATLVFGVVAYAGGWMLPNLVWYVVLAFWLAIMPLWLRFKWRLN
                     GGWQVYAVGWLLVMPFWFALVSLRPHPDDALPLLAVMGLVWVADVCAYFSGKAFGKHK
                     IAPAISPGKSWEGAIGGAVCVAVYMTAVRSAGWLAFDTGWFDTVLIGLVLTVVSICGD
                     LLESWLKRAAGIKDSSNLLPGHGGVFDRTDSLIAVISVYAAMMSVLN"
     gene            5892..7127
                     /gene="ispC"
                     /locus_tag="ACFM8S_000734"
     CDS             5892..7127
                     /gene="ispC"
                     /locus_tag="ACFM8S_000734"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFC6313837.1"
                     /translation="MPSENASDGIRYKVIPIMTPQVLTILGSTGSIGESTLDVVSLHP
                     EKFRVFALAGHKQVEKLAVQCRTFRPEYAVVADAEHAARLEALLKGDGTATQVLHGAQ
                     ALVDVASADEVSGVMCAIVGAVGLPSALAAAQKGKTIYLANKETLVVSGALFMETARA
                     NGAAVLPVDSEHNAVFQVLPRDYAGRLNEHGIASIILTASGGPFLTADLNTFDSITPD
                     QAVKHPNWRMGRKISVDSATMMNKGLELIEAHWLFNCPPDKLEVVIHPQSVIHSMVRY
                     RDGSVLAQLGNPDMRTPIAYCLGLPERIDSGVGDLDFDALSALTFQKPDFDRFPCLKL
                     AYEAMNAGGAAPCVLNAANEAAVAAFLDGQIKFTDIAKTVAHCLSQDFSDGIGDIGGL
                     LAQDARTRAQARAFIGTLR"
     gene            7162..8502
                     /gene="rseP"
                     /locus_tag="ACFM8S_000735"
     CDS             7162..8502
                     /gene="rseP"
                     /locus_tag="ACFM8S_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFC6313838.1"
                     /translation="MHTLLAFIFAILILVSLHEFGHYIVARLCGVKVVRFSVGFGKPF
                     FTRKRGDTEWCLAPIPLGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLT
                     NLALAVLLYGLSFSFGVTELRPYVGTVEPDTIAARAGFQSGDKIQSVNGTPVADWGGA
                     QTEIVLNLEAGKVAVGVQTASGAQTVRTIDAAGTPEAGKIAKNQGYIGLMPFKITTVA
                     GGVEKGSPAEKAGLKPGDRLTAADGKPIASWQEWANLTRQSPGKKITLNYERAGQTHT
                     ADIRPDTVEQSDHTLIGRVGLRPQPDRAWDAQIRRSYRPSVIRAFGMGWEKTVSHSWT
                     TLKFFGKLISGNASVSHISGPLTIADIAGQSAELGLQSYLEFLALVSISLGVLNLLPV
                     PVLDGGHLVFYTAEWIRGKPLGERVQNIGLRFGLALMMLMMAVAFFNDVTRLLG"
     gene            8559..10952
                     /gene="bamA"
                     /locus_tag="ACFM8S_000736"
     CDS             8559..10952
                     /gene="bamA"
                     /locus_tag="ACFM8S_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFC6313839.1"
                     /translation="MKLKQIASALMVLGISPLALADFTIQDIRVEGLQRTEPSTVFNY
                     LPVKVGDTYNDTHGSAIIKSLYATGFFDDVRVETADGQLLLTVIERPTIGSLNITGAK
                     MLQNDAIKKNLESFGLAQSQYFNQATLNQAVAGLKEEYLGRGKLNIQITPKVTKLARN
                     RVDIDITIDEGKSAKITDIEFEGNQVYSDRKLMRQMSLTEGGIWTWLTRSNQFNEQKF
                     AQDMEKVTDFYQNNGYFNFRILDTDIQTNEDKTKQTIKITVHEGGRFRWGKVSIEGDT
                     NEVPKAELEKLLTMKPGKWYERQQMTAVLGEIQNRMGSAGYAYSEISVQPLPNAETKT
                     VDFVLHIEPGRKIYVNEIHITGNNKTRDEVVRRELRQMESAPYDTSKLQRSKERVELL
                     GYFDNVQFDAVPLAGTPDKVDLNMSLTERSTGSLDLSAGWVQDTGLVMSAGVSQDNLF
                     GTGKSAALRASRSKTTLNGSLSFTDPYFTADGVSLGYDVYGKAFDPRKASTSIKQYKT
                     TTAGAGIRMSVPVTEYDRVNFGLVAEHLTVNTYNKAPKHYADFIKKYGKTDGTDGSFK
                     GWLYKGTVGWGRNKTDSALWPTRGYLTGVNAEIALPGSKLQYYSATHNQTWFFPLSKT
                     FTLMLGGEVGIAGGYGKTKEIPFFENFYGGGLGSVRGYESGTLGPKVYDEYGEKISYG
                     GNKKANVSAELLFPMPGAKDARTVRLSLFADAGSVWDGKTYDDNSSSATGGRVQNIYG
                     AGNTHKSTFTNELRYSAGGAVTWLSPLGPMKFSYAYPLKKKPEDEIQRFQFQLGTTF"
     gene            11018..11518
                     /locus_tag="ACFM8S_000737"
     CDS             11018..11518
                     /locus_tag="ACFM8S_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HFC6313840.1"
                     /translation="MTRLTRAFAAALIGLCCTAGAHADTFQKIGFINTERIYLESKQA
                     RKIQKTLDSEFSARQDELQKLQREGLDLERQLAEGKLRNAKKAQAEEKWRGLVAAFRK
                     KQAQFEEDYNLRRNEEFASLQQNANRVIVKIAKQEGYDVILQNVIYVNTQYDVTDSVI
                     KEMNAR"
     gene            11551..12594
                     /gene="lpxD"
                     /locus_tag="ACFM8S_000738"
     CDS             11551..12594
                     /gene="lpxD"
                     /locus_tag="ACFM8S_000738"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFC6313841.1"
                     /translation="MIPATYTLSQITARLGGEWRGEDISVTAVRPLADAQAEHISFLA
                     NPKYKAEVHDSSAGAVIVSAKAADGFEGRNLIVADDPYLYFAKVARLFSPVVKARGGI
                     HPTAVVEPGATVPASCEIGANVYIGANTVLGEGCRILANAVVQHDCKLGDEVVLHPNA
                     VVYYGCTLGRRVEIHSGAVIGADGFGLAFAGDSWFKIPQTGAVTLGDDVEIGSNTNID
                     RGAMSDTTVGNGTKIDNQVQIGHNCKIGSHTVIAAKTGISGSVTIGSYCIIGGGVGTV
                     GHIEIADKTTIGGGTSVTHSITESGKHLAGIFPMSTHKEWARNAVYIHRLSEMNKRLK
                     TLEQQLSDGKDTQ"
     gene            12630..13079
                     /gene="fabZ"
                     /locus_tag="ACFM8S_000739"
     CDS             12630..13079
                     /gene="fabZ"
                     /locus_tag="ACFM8S_000739"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFC6313842.1"
                     /translation="MDVQLPIEAKDIQKLIPHRYPFLQLDRITAFEPMKTLTAIKNVT
                     INEPQFQGHFPDLPVMPGVLIIEAMAQACGTLAILSEGGRKENEFFFFAGIDEARFKR
                     QVIPGDQLVFEVELLTSRRGIGKFNAVAKVDGQVAVEAIIMCAKRVV"
     gene            13287..13967
                     /locus_tag="ACFM8S_000740"
     CDS             13287..13967
                     /locus_tag="ACFM8S_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HFC6313843.1"
                     /translation="MERYKNAVGEDKAAELYLLNLSLSRELFHVVSIFEIVLRNKIDI
                     CLQQAFKDRNWLYNSIQPQTNPALKYQGCFLRNGTKESAELIKVALSKIQNNSGGKFD
                     HNQLVAGLGFGFWRYLFAGGKDAQFDATGKVLMKVFPKKPKSTPSVQYNQKWIFRELS
                     NINKFRNRLAHHEPICFKGAIKDTNYARNIHQSIFELLNYMDVDTASVFSHFSDQVIA
                     VCDEIDKL"
     gene            14010..14786
                     /gene="lpxA"
                     /locus_tag="ACFM8S_000741"
     CDS             14010..14786
                     /gene="lpxA"
                     /locus_tag="ACFM8S_000741"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFC6313844.1"
                     /translation="MTLIHPTAVIDPKAELDSSVKVGAYTVIGPNVQIGANTEIGPHA
                     VINGHTSIGENNRIFQFASLGEIPQDKKYRDEPTKLIIGNGNTIREFTTFNLGTVTGI
                     GETRIGDDNWIMAYCHLAHDCVVGNHTIFANNASLAGHVTIGDYVVLGGYTLVFQFCH
                     IGDYAMTAFAAGVHKDVPPYFMASGYRAEPAGLNSEGMRRNGFTAEQISAVKDVYKTL
                     YHRGIPFEEAKADILRRAETQAELAVFRDFFAQSTRGIIR"
BASE COUNT         3771 a         4657 c         3743 g         2956 t
ORIGIN      
        1 cgatataagt atttttattt taatccgcca tattaacgca cccggccaaa cagcataaag
       61 gcacgggcag cccgatttgg attgacctat ttccccgaca ccctcgccgc cagctcgtcc
      121 gccaaggcaa gatacgcctt ggtacccttt gcctgcgcat cataagccat caccggcata
      181 ccgtggctcg gcgcttccgc aaggcggata ttgcgcggga tgacggtttc aaacagcaaa
      241 tccccgaaat ggctgcgcaa ctgttcgctg acttcggcaa ccagcctgct gcggctgtcg
      301 tacatcgtac gcacgatgcc cgtgatgtcc aaatcgggat tgaccgcctg acggattttg
      361 cgcacggtcg caatcaaatc ggaaatccct tccagcgcgt aatattcgca caacatcggc
      421 acaatcacgc cgccggctgc caccagcccg ttaagcgtca acagcgtcag cgaaggcgga
      481 cagtcgatca ggataaagtc gtaatcttcc gccactgcct tgagtgcgtt tttcaaacgc
      541 acttcccggg cgatttcctg caccagttcg atttccgcgc cggcaagtgc gcggttcgca
      601 cccaacacag cgtatccgcc ctctttgccg cgtaccgccg ccgatttcac gtccgcatcg
      661 cccaataaga cctgataaac gccggactgc aaacccgcct tgtcgatacc gctgcccgtc
      721 gtcgcattgc cctgcggatc caaatcgacc accagcacgc gtttgccgcg cgatgccagc
      781 gaagccgcca aatttaccgt cgtcgtcgtt ttgcccacac cgcccttctg attggcgatg
      841 gcaaggatgt tcgcactcat gtcccgcccg tgtcccgtct gatgttgaaa aaagaaaaac
      901 agtgcggaat tttaccttta tccgcacaaa aaaggtattc atgccgtctg aagtgcgctc
      961 aacgcttact caggatgacg atatggcgtt ccgcatccaa gcccggcacg tcgagccttt
     1021 ggactttttc aacgcacaca tcctgcggca ggcggccgat ttcttcctgc ggatacacgc
     1081 ccttcatcgc cgcccagtag ccgccgtctt tcaacagatg caccgtccac gacacaaaat
     1141 ccgccagttc tgcaaacgca cggctggtaa ccacatcggc acgcacgtcc gaaaccgcct
     1201 ccacgcgtcc ggataccacg cgcacattgt ccaaccccaa ctcgataacc gcctgctgta
     1261 aaaaagccgt tttcttcgta ttcgcatcca aaagggttat ctgcacatcc ggacggcaca
     1321 ccgccgccgg aatgccgggc tgaccgccgc ccgaaccgac atccagcatc gtttgcacac
     1381 cctcgatatg gggcagcagc gtcaggctgt ccaaaagatg atggacaatc attttttcct
     1441 cgtcgcgcag ggcggtcaga ttgtaggttt tgttccactt tttcaacaaa tccacatagg
     1501 ccaaaagcct gtcctgcgcc gtttccgaaa tatccagccc catcgcagca atgcccgatt
     1561 gtaggcgttc tttacgttcc atatctgctt cctatccata ttcaggcgta atgttagcgg
     1621 aaaaacatgc cgtctgaaac cgcgtgtcgg gtttcagacg gcatttcccg ttccgtccta
     1681 ttttttcctg tggaacacca ccagctgcac tggcgcgcgc gtcaagcgtt tgggcagcgt
     1741 cagcatcgcc accagccaca cggcaaacgc atattccaac accgcctccc cgcgcagccc
     1801 gcccaaggcg gcactgccca acaacagttc gaaaatccaa gccaccgccg ccgccgcgca
     1861 agccgccacg cctgacagaa acagcgcgta acgcgaatgc cgccgcatca tctttttgta
     1921 cgccagtttc gtcagacccg ccgacacaac cacattcaaa aacgcaaaca caccgaacac
     1981 aaaccaaaca gtgctgtccc aaccgccacg ccagccgaat ccgccgtccg acaccgacac
     2041 cagcagcccc gtccgtccga acgcaaatgc caaggcatca tgcaccgaca gtgcgaacgg
     2101 cagcgtcaaa aacaccagcc ccgcaaacaa agccgcatag ggatatttct cgttcacata
     2161 catcaccgac tctcctcacg aagcaaaccg cattataccg tgcaaaactt tgccgcccgc
     2221 ccgaaaagtt gcacaaacaa ccgttcatat atatcatgac gaaaaaacgc cggtgtagct
     2281 cagtcggtag agcagcgcat tcgtaacgcg aaggtcgggg gttcgattcc cttctccggc
     2341 accaatacca agcacagacc ctcccttcct cgggaagcct gtgctttttc acatttccgc
     2401 ttcagacggc acaaccgata tgaacacctc gcaacgcaac cgcctcgtca gccgctggct
     2461 caactcctac gaacgctacc gctaccgccg cctcatccac gccgtccggc tcggcggggc
     2521 cgtcctgttc gccaccgcct ccgcccggct gctccacctc caacacggcg agtggatagg
     2581 gatgaccgtc ttcgtcgtcc tcggcatgct ccagtttcaa ggggcgattt actccaaggc
     2641 ggtggaacgt atgctcggca cggtcatcgg gctgggcgcg ggtttgggcg ttttatggct
     2701 gaaccagcat tatttccacg gcaacctcct cttctacctc accgtcggca cggcaagcgc
     2761 actggccggc tgggcggcgg tcggcaaaaa cggctacgtc cctatgctgg cggggctgac
     2821 gatgtgcatg ctcatcggcg acaacggcag cgaatggttc gacagcggcc tgatgcgcgc
     2881 gatgaacgtc ctcatcggcg cggccatcgc catcgccgcc gccaaactgc tgccgctgaa
     2941 atccacactg atgtggcgtt tcatgcttgc cgacaacctg accgactgca gcaaaatgat
     3001 tgccgaaatc agcaacggca ggcgcatgac ccgcgaacgc ctcgaagaga acatggcgaa
     3061 aatgcgccaa atcaacgcac gcatggtcaa aagccgcagc cacctcgccg ccacatcggg
     3121 cgaaagccgc atcagccccg ccatgatgga agccatgcag cacgcccacc gtaaaattgt
     3181 caacaccacc gagctgctcc tgaccactgc cgccaagctg caatctccca aactcaacgg
     3241 cagcgaaatc cggctgctcg accgccactt cacactgctc caaaccgacc tgcaacaaac
     3301 cgtcgccctt atcaacggca gacacgcccg ccgcatccgc atcgacaccg ctatcaaccc
     3361 cgaactggaa gccctcgccg aacacctcca ctaccaatgg cagggcttcc tctggctcag
     3421 caccaatatg cgtcaggaaa tttccgccct cgtcatcctg ctgcaacgca cccgccgcaa
     3481 atggctgaat gcccacgaac gccaacacct gcgccaaagc ctgcttgaaa cacgggaaca
     3541 cggctgacgg ccggacacga tgccgtctga aatgcctgcc gcttcagacg gcatccccaa
     3601 tccgcctgcc ttgtcgtcat gcccgaataa cggtactatt cgcagttaac agttttcatg
     3661 ccgtctgaaa ggtttgaagc gcgtttcaga cggctgcccg cctaccttat cttaccgaaa
     3721 gaacaatatg atcaacgata ttcaaaaaac agccgaaggc aaaatgcagc gttcggtcga
     3781 agtactgaaa gaaaatctgg cgaaagtgcg taccggccgc gcgcataccg gcctgctcga
     3841 ccaagtggaa gtcgaatact ggggcagcat ggtccccgtc agccaggttg ccaacgtaac
     3901 gcttctggac gcgcgcacca tcggcgtgaa accgtttgag ggcaatatgg cggccaaagt
     3961 cgagaaagcc atccgcgatt caaacttggg gctgaacccg gcagctgtcg gcgacctgat
     4021 ccgcgtaccg atgcccatgc tgaccgagga acgccgcaaa gacctgatta aagtcgtacg
     4081 cggcgaagcg gaagaaggcc gcgtctccat ccgcaacgtg cgccgcgatg ccaacgacca
     4141 catcaaaaaa ctcctcaaag acaaagaaat ttccgaagac gaggcacgtc gcggcgaaga
     4201 agcggttcaa aaactgaccg acaaatacat taccgaagcc gacaaactcc tgactgccaa
     4261 agaagaagat ttgatggcaa tttaacctgc acggttcggc gttcagacgg catttgaacg
     4321 ccgaaccgcg aaaggcagac atgaaaagca gcacgcaggc cgttttggaa cacaccgcca
     4381 ttcccaagca tatcgccgtg attatggacg gcaacggccg ttgggcgaaa aaacgtttcc
     4441 tcccgcgcat aatgggacac aaacgcggtt tggacgcgtt ggaaaatatg gtgaagcatt
     4501 gcgccaaact gggtgtgcaa tatctgaccg tgtttgcctt ttcaaccgaa aactggcgcc
     4561 gccccgaaga cgaagtttcg ttcctgatgg ggctgttttt acaggctttg caaaaacagg
     4621 tacgccgcct gcacgaaaac aatatgcgcc tgaaaatact gggcagccgc gaacgcttta
     4681 atcggcagat tctgcaaggc atcgaagagg cggaagcctt gacggcaaac aataccggcc
     4741 tgaccctgag cattgccgcc gattacggcg gccgctggga tattttgcag gcggcaaaca
     4801 aactgattgc cgaaggcgta tccgagatta cggaagacac gctggcgaaa cacttgatgc
     4861 tgggcgatgc accggaaccg gatttgttca tccgcaccgg cggcgaaacg cgcatcagca
     4921 atttcctgct ctggcagatg gcatatgcag aactgtattt caccgatatt ttgtggcccg
     4981 attttgacgg caaggctttg gacgatgccg tcgcttcgtt ccaaaaacgc gaacggcggt
     5041 tcggacgcac ctccgaacaa ctgcctatcg aacagcaaag gaactgaata tgctgaaaca
     5101 acgggtaata accgccatgt ggctgctgcc gctgatgctg ggcatgctgt tttacgcgcc
     5161 gcaatggttg tgggccgcat tttgcggact gattgccctg attgccttgt gggaatatgc
     5221 ccgtatgggc ggcttgtgca aaatcaaaac caaccattac ctcgtcgcaa ccttggtttt
     5281 cggcgtggtt gcctatgcgg gcggctggat gctgcctaat ttggtttggt atgttgtttt
     5341 ggcattttgg ctcgccatca tgcctttatg gttgagattt aaatggaggc tcaacggcgg
     5401 ttggcaggtt tatgccgtcg gctggcttct ggtcatgccg ttttggttcg cgctcgtatc
     5461 cctgcgcccg catcccgatg atgccctgcc gctgctcgcc gtgatgggtc tggtctgggt
     5521 tgccgacgtt tgcgcgtatt tcagcggcaa ggcgttcggc aaacacaaaa tcgcaccggc
     5581 aatcagcccc ggcaaaagct gggaaggcgc aatcggcggt gcggtttgcg tggccgtgta
     5641 catgactgcc gtacgaagtg ccggctggct ggcattcgat acaggctggt tcgataccgt
     5701 gttaatcggt ttggtgctga ccgtcgtcag catatgcggc gacctgttgg aaagctggct
     5761 caagcgcgcg gcaggcatca aagacagcag caacctgctg ccaggacacg gcggcgtgtt
     5821 cgaccgcacc gacagcctga ttgccgttat cagcgtctat gcggcgatga tgtcggtttt
     5881 aaattgattc tatgccgtct gaaaacgctt cagacggcat ccggtataaa gttatcccca
     5941 ttatgacacc acaagtcctg accatattag gcagtaccgg cagcataggc gaaagcacgc
     6001 tggacgttgt ctccctccac cccgaaaaat tccgcgtatt cgcgctggca gggcataagc
     6061 aggtcgagaa actggcggtg caatgccgga cgttccgccc cgaatatgcc gtcgttgccg
     6121 atgccgaaca cgccgcccgg cttgaagctt tgttgaaagg cgacggcacg gcgacgcagg
     6181 ttttacacgg cgcgcaggca ttggttgacg ttgcctctgc cgacgaagtc agcggtgtca
     6241 tgtgcgccat cgtcggggcg gtggggctgc cttccgcgct cgcagcggcg caaaaaggca
     6301 aaaccattta tctggcgaac aaagagacgc tggtggtttc cggcgcgttg tttatggaaa
     6361 ccgcccgtgc aaacggcgcg gcagtgctgc ccgtcgacag cgaacacaac gccgttttcc
     6421 aagttttgcc gcgcgattac gccggccgtc tgaacgaaca cggcatcgct tcgattatcc
     6481 tgaccgcttc cggcggcccg tttctgaccg ccgatttaaa cacgttcgac agcattacgc
     6541 ccgaccaagc ggtcaaacac cccaattggc gtatgggacg caaaatctcc gtcgattccg
     6601 ccaccatgat gaacaaaggt ttggagctga ttgaagcgca ttggctgttc aactgtccgc
     6661 ccgacaaact cgaagtcgtc atccatccgc aatccgtgat acacagcatg gtgcgctacc
     6721 gcgacggctc cgtgttggcg caactgggca atcccgatat gcgaacgccc atcgcttatt
     6781 gtttgggttt gcccgagcgc atcgattcgg gtgtcggcga cctggatttc gacgcattgt
     6841 ccgcgctgac cttccaaaag cccgactttg accgcttccc ctgcctgaag ctcgcctatg
     6901 aagccatgaa cgcaggcgga gccgcgccct gcgtattgaa cgccgccaac gaagccgccg
     6961 tcgccgcctt tttggacgga cagattaagt ttaccgacat tgccaaaacc gtcgcccatt
     7021 gtctttcaca agacttttca gacggcatag gcgacatagg ggggctcttg gcgcaagatg
     7081 cccggacacg cgcacaagcg cgggcattta tcggcacact gcgctgatgc cgtctgaaca
     7141 ccgttatcaa aggaaaacca tttgcacacc cttctagctt ttatcttcgc catcctgatt
     7201 ttggtcagcc tgcacgagtt cggacactac atcgttgcca gattgtgcgg cgtcaaagtc
     7261 gtacgctttt ccgtcggctt cggcaaaccg tttttcaccc gaaagcgcgg cgacaccgaa
     7321 tggtgcctcg ccccgattcc gttgggcggt tacgtcaaaa tggtcgacac gcgcgaaggc
     7381 gaagtatcag aagccgattt accctacgct tttgacaaac aacaccccgc caagcgcatc
     7441 gccatcgtcg ccgccggccc actgaccaac ctcgcactgg cggttttgct gtacggactg
     7501 agcttttcct tcggcgtaac cgaactgcgc ccctacgtcg gtacagtcga acccgacacc
     7561 attgccgccc gcgccggctt ccaaagcggc gacaaaatac aatccgtcaa cggcacaccc
     7621 gttgcagatt ggggcggcgc gcaaaccgaa atcgtcctca acctcgaagc cggcaaagtc
     7681 gccgtcggcg ttcagacggc atcgggcgcg caaaccgtcc gcaccatcga tgccgcaggc
     7741 acgccggaag ccggtaaaat cgcaaaaaac caaggctaca tcggactgat gccctttaaa
     7801 atcacaaccg ttgccggcgg cgtggaaaaa ggcagccccg ccgaaaaagc aggcctgaaa
     7861 ccgggcgaca ggctgactgc cgccgacggc aaacccatcg cctcatggca agaatgggca
     7921 aacctgaccc gccaaagccc cggcaaaaaa atcaccctga actacgaacg cgccggacaa
     7981 acccataccg ccgacatccg ccccgatact gtcgaacagt ccgaccacac cctgatcggg
     8041 cgcgtcggcc tccgtccgca gccggacagg gcgtgggacg cgcaaatccg ccgcagctac
     8101 cgtccgtctg ttatccgcgc attcggcatg ggctgggaaa aaaccgtttc ccactcgtgg
     8161 acaaccctca aatttttcgg caaactaatc agcggcaacg cctccgtcag ccatatttcc
     8221 gggccgctga ccattgccga cattgccgga cagtccgccg aactcggctt gcaaagttat
     8281 ttggaatttt tggcactggt cagcatcagc ctcggcgtgc tgaacctact gcccgtcccc
     8341 gttttggacg gcggccacct cgtgttttat actgccgaat ggatacgcgg caaacctttg
     8401 ggcgaacgcg tccaaaacat cggtttgcgc ttcgggcttg ccctcatgat gctgatgatg
     8461 gcggtcgcct tcttcaacga cgttacccgg ctgctcggtt agattttacg tttcggaatg
     8521 ccgtctgaaa ccgcattccg caccacaagg aactgacaat gaaactgaaa cagattgctt
     8581 ccgcactgat ggtcttgggc atatcgcctt tggcacttgc cgacttcacc atccaagaca
     8641 tccgcgtcga aggcttgcag cgtaccgagc cgagtaccgt attcaactac ctgcccgtca
     8701 aagtcggcga cacctacaac gacacacacg gcagtgccat catcaaaagc ctgtacgcca
     8761 ccggtttctt tgacgacgta cgcgtcgaga ctgcggacgg gcagctcctg ctgaccgtca
     8821 tcgaacgccc caccatcggc tcgctcaaca tcaccggcgc aaaaatgctg caaaacgacg
     8881 ccattaagaa aaatctcgaa tcgttcgggc tggcgcagtc gcaatacttt aatcaggcga
     8941 cactcaatca ggcagtcgcc ggcctgaaag aagaatacct cgggcgcggc aaactcaata
     9001 tccaaatcac gcccaaagta accaaactcg cccgcaaccg cgtcgacatc gacatcacga
     9061 ttgacgaggg caaatccgcc aaaatcaccg acatcgaatt tgaaggcaac caagtctatt
     9121 ccgaccgcaa actgatgcgg cagatgtcgc tgaccgaagg cggcatttgg acatggctga
     9181 cacgaagcaa ccaattcaac gagcagaaat ttgcccaaga catggaaaaa gtaaccgact
     9241 tctaccagaa caacggctac ttcaatttcc gcatcctcga taccgacatc caaaccaacg
     9301 aagacaaaac caagcagacc atcaaaatca ccgtccacga aggcggacgt ttccgttggg
     9361 gcaaagtctc catcgaaggc gacaccaacg aagtccccaa agccgaactg gaaaaactgc
     9421 tgaccatgaa gcccggcaaa tggtacgaac gccagcagat gaccgccgtt ttgggtgaga
     9481 ttcagaaccg catgggctcg gcaggctacg catacagcga aatcagcgta cagccgctgc
     9541 caaacgccga aaccaaaacc gtcgatttcg tcctgcacat cgaaccgggc cggaaaatct
     9601 acgtcaacga aatccacatc accggcaaca acaaaacccg cgacgaagtc gtgcgccgcg
     9661 aattgcgcca aatggaatcc gcgccttacg acacctccaa gctgcaacgc tccaaagagc
     9721 gcgtcgagct tttgggctac ttcgacaacg tacagtttga tgccgtcccg cttgccggca
     9781 cacccgacaa agtcgatttg aacatgagcc tgaccgaacg ttccaccggc tcgctcgact
     9841 tgagcgcggg ctgggtacag gataccggcc tggtcatgtc cgcaggcgtt tcccaagaca
     9901 acctgttcgg tacgggcaag tcggccgccc tgcgcgcctc acgaagcaaa accacgctca
     9961 acggctcgct gtcgtttacc gacccgtact tcacggcaga cggggtcagc ctgggctacg
    10021 atgtttacgg aaaagccttc gacccgcgca aagcatcgac cagcatcaaa caatataaaa
    10081 ccaccacggc aggcgcaggc atccgcatga gcgtgcctgt taccgaatac gaccgcgtga
    10141 atttcggttt ggtggcagaa cacctgaccg tcaacaccta caacaaagcg cccaaacact
    10201 atgccgactt tatcaagaaa tacggcaaaa ccgacggcac agacggcagc ttcaaaggct
    10261 ggctgtacaa aggtaccgtc ggctgggggc gcaacaaaac cgacagcgcg ttatggccga
    10321 cgcgcggcta cctgacgggc gtgaacgccg aaatcgccct gcccggcagc aaactgcaat
    10381 actactccgc cacccacaac caaacctggt tcttcccctt aagcaaaacc ttcacactga
    10441 tgctgggcgg cgaagtcggc attgcgggcg gctacggcaa aaccaaagaa atccccttct
    10501 ttgaaaactt ctacggcggc ggcctgggtt cggtgcgcgg atacgaaagc ggcacgctcg
    10561 gtccgaaagt gtatgacgaa tacggcgaaa aaatcagcta cggcggcaac aaaaaagcca
    10621 acgtctccgc cgagctgctc ttcccgatgc ccggcgcgaa agacgcgcgc accgtccgcc
    10681 tgagcctgtt tgccgacgca ggcagcgtgt gggacggcaa aacctacgac gacaacagca
    10741 gttccgcgac cggcggcagg gttcaaaaca tttacggcgc cggcaatacc cataaatcca
    10801 cctttaccaa cgaattgcgc tattccgccg gcggcgcggt tacctggctc tcgcctttag
    10861 gcccgatgaa attcagctac gcctacccgc tgaagaaaaa accggaagac gaaatccaac
    10921 gcttccaatt ccaactcggc acgacgttct aatcccgcaa atgccgtctg aagcccttca
    10981 gacggcattt cgcggcaaca ttcgaaggag ttttaccatg acccgtttga cccgcgcgtt
    11041 tgccgcggct ctgatcggtt tgtgctgcac cgcaggcgcg cacgccgaca ccttccaaaa
    11101 aatcggcttt atcaacaccg agcgcatcta cctcgaatcc aagcaggcgc gcaagattca
    11161 aaaaacgctg gacagcgaat tttccgctcg tcaggacgaa ttgcaaaaac tgcaacgcga
    11221 aggtctggat ttggaaaggc agcttgccga aggcaaactc agaaacgcaa aaaaggcgca
    11281 agccgaagaa aaatggcgcg ggctggtcgc agcgttccgc aaaaaacagg cgcagtttga
    11341 agaagactac aacctccgcc gcaacgaaga gtttgcctcc ctccagcaaa acgccaaccg
    11401 cgtcatcgtc aaaatcgcca aacaggaagg ttacgatgtc attttgcaga acgtgattta
    11461 cgtcaacacc caatacgacg ttaccgacag cgtcattaaa gaaatgaacg cccgctgacc
    11521 ctttcagacg gcataccgaa caggaaaacc atgattccgg ccacctacac cctgtcccaa
    11581 atcaccgcgc ggctcggcgg cgaatggcgc ggcgaggaca tttccgttac cgccgtgcgc
    11641 ccgctcgcag acgcgcaggc ggaacacatc agcttccttg ccaatccgaa atacaaagcc
    11701 gaagtccacg acagcagcgc gggcgcggtc atcgtttccg ccaaagcggc agacggattt
    11761 gaagggcgca acctgattgt cgccgacgac ccctatctct atttcgccaa agtcgcccgc
    11821 ctgttttcac ccgtcgtcaa agcgcgcggc ggcatccatc cgaccgccgt cgtcgaaccg
    11881 ggcgcgaccg ttcccgccag ctgcgaaatc ggcgcgaacg tctacatcgg cgcaaacacc
    11941 gtgctcggcg aaggctgccg catcttggca aacgccgtcg tccaacacga ttgcaaactg
    12001 ggcgacgaag tcgtcctgca tcccaacgcc gtcgtttatt acggctgcac actgggcaga
    12061 cgcgtcgaaa tccacagcgg cgcggtcatc ggcgcggacg gtttcggact cgcttttgcc
    12121 ggcgattcat ggtttaaaat cccgcaaacc ggcgcggtaa cgctgggcga cgacgtagaa
    12181 atcggctcga acaccaacat cgaccgcggc gcgatgagcg acaccaccgt cggcaacggc
    12241 accaaaatcg acaaccaagt ccaaatcgga cacaactgca aaatcggttc gcacaccgtc
    12301 atcgccgcca aaaccggcat ctcaggcagc gtaaccatag gcagctactg catcatcggc
    12361 ggcggcgtcg gtacggtcgg acacatcgaa atcgccgaca aaaccaccat cggcggcggc
    12421 acatccgtca cccacagcat taccgaaagc ggcaaacacc ttgccggcat cttcccgatg
    12481 tccacccata aagaatgggc gcgcaacgcc gtttacatcc accgcttaag cgaaatgaac
    12541 aaacgcctca aaacgctgga acagcagctt tcagacggca aagacacgca ataaatccaa
    12601 accgacttta ttcaaggaat acgacagaca tggacgttca actccccatc gaagccaaag
    12661 acatccaaaa actcatcccc caccgctatc cgtttctcca gctcgaccgc atcaccgcct
    12721 tcgagccgat gaaaaccctg accgcgatta aaaacgtcac cataaacgaa ccccagttcc
    12781 aaggccattt tcccgacctg cccgtgatgc ccggcgtact catcatcgaa gcgatggcgc
    12841 aggcgtgcgg cacgttggcg attttgagcg aaggcgggcg caaagaaaac gaattcttct
    12901 tcttcgccgg catagacgaa gcccgtttca aacgccaagt catccccggc gaccaactcg
    12961 tctttgaagt cgagctgctg accagccggc gcggcatcgg caaattcaac gccgttgcca
    13021 aagtggacgg gcaagtcgcc gtcgaagcca tcatcatgtg tgccaaacgc gtggtttgag
    13081 tgttcagaaa aaggtcgtct gaaagttttc agacgaccta ataaaaagaa aacccaccgg
    13141 tttaggttta ttcaaaccca gggacacggt ggggattagt tttacctaac tgtatcaaat
    13201 atagtttgaa acgtcaattt aaaaataaag aatcaggtgt tagcgtggat tataaggaat
    13261 gctgtagtat tttttctgat ttcagaatgg aacggtataa aaatgccgtt ggggaagaca
    13321 aagcagctga attgtattta ttgaatttat ctttatcaag agaacttttt catgttgttt
    13381 caatatttga gattgtttta agaaacaaaa ttgacatttg ccttcagcag gcgtttaaag
    13441 acagaaactg gttatataac agcatacagc cacagacgaa tcccgcatta aaatatcaag
    13501 gttgcttttt aagaaatggc acgaaagaat ctgctgaatt aatcaaggtt gcattatcta
    13561 aaatacaaaa taatagcggg ggaaagtttg accacaacca attggttgca ggactaggat
    13621 tcggtttttg gcggtatctg tttgcaggag ggaaagatgc ccaatttgat gctacaggga
    13681 aagtattgat gaaagttttt ccaaaaaagc ccaaatctac accaagtgtg caatataacc
    13741 aaaaatggat ttttcgagaa ctttccaata ttaacaaatt ccgtaatcgt ttggcacatc
    13801 acgagccgat ttgttttaaa ggtgcaataa aagataccaa ctacgctaga aatattcacc
    13861 aatctatttt tgaactgctt aattacatgg atgtagatac tgcttcagta tttagccatt
    13921 ttagcgatca agtgattgcg gtctgcgatg aaatcgacaa actgtaattt tctattattt
    13981 gtcttaacaa gcagcggagt attcaaaaca tgaccctcat ccacccgacc gccgtcatcg
    14041 accccaaagc cgaactcgac tccagcgtca aagtcggcgc gtacaccgtc atcggcccca
    14101 acgtccaaat cggcgcgaat accgaaatcg gcccgcacgc cgtcatcaac ggccacacca
    14161 gcatcggcga aaacaaccgc attttccaat ttgccagcct cggcgaaatc ccgcaggaca
    14221 aaaaataccg cgacgagccg accaagctga ttatcggcaa cggcaacacc atccgcgaat
    14281 tcaccacctt taatttaggc acggtaaccg gcatcggcga aacccgtatc ggcgacgaca
    14341 actggattat ggcgtactgc cacctcgcgc acgactgcgt ggtcggcaac cacaccatct
    14401 tcgccaacaa cgcctcgctt gccggacacg ttaccatcgg cgactacgtc gttttgggcg
    14461 gctacacgct ggttttccaa ttctgccaca tcggcgacta cgctatgacc gccttcgccg
    14521 caggcgtaca caaagacgta ccgccctact ttatggcatc gggctaccgc gccgaaccgg
    14581 cggggctcaa cagcgaaggc atgcgccgca acggttttac cgcagaacag atttccgccg
    14641 tcaaagacgt gtacaaaacc ctctaccatc gcggcattcc gtttgaagag gccaaggcag
    14701 acatcctccg ccgcgccgaa acccaagccg agcttgccgt attccgagac tttttcgcac
    14761 aatcgacgcg cggcatcatc cgctgaccgt acccttttga tgccgtctga aaccccgaca
    14821 gcgtttcaga cggcatcggt ttttttaaag cggtatattg tttgatcgat gcatcatttt
    14881 gtagatttca gaagtatggc agtcggcagc attgatgatt tctgccgccc gccacatccc
    14941 cattcggaca tttcattgtg acctccgccg ctgcttttaa agaattacaa acaatttccg
    15001 cagcttgaac cggcctgttt tcggcgaaaa aaaccaaaag tatttttcaa aagaacatat
    15061 tgtcttattg gatattctgt gctgaaatta ctggacacta caaaagtggt gttttaaaaa
    15121 tatagtg
//
LOCUS       DAXHSF010000030        14954 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.030, whole genome shotgun sequence.
ACCESSION   DAXHSF010000030 DAXHSF010000000
VERSION     DAXHSF010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14954
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.030"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(<1..104)
                     /locus_tag="ACFM8S_000742"
     CDS             complement(<1..104)
                     /locus_tag="ACFM8S_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFC6313845.1"
                     /translation="MNTNQPAVYDPLTRALHWLTVAGFIGILTTIVLWT"
     gene            382..1536
                     /gene="obgE"
                     /locus_tag="ACFM8S_000743"
     CDS             382..1536
                     /gene="obgE"
                     /locus_tag="ACFM8S_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFC6313846.1"
                     /translation="MKFIDEAKIEVAAGKGGNGATSFRREKFVPRGGPDGGDGGKGGS
                     VWAEADENTNTLVEYRFVKRYQAKNGEKGHGSDRYGAGADDIVLKMPVGTLIRDLDTD
                     EIVADLTYHGQRVCLAKGGKGGLGNIHFKSSVNRAPKQSTPGEEGETRSLQLELKVLA
                     DVGLLGMPNAGKSTLITAVSAARPKIANYPFTTLHPNLGVVRIDENHSFVMADIPGLI
                     EGAAEGAGLGHRFLKHLSRTGLLLHVVDLAPFDEAVDPAEEALAIINELRKYDEELYG
                     KPRWLVLNKLDMLDEEEAQERTVTFLEAVGWDYPKPDDRFQFDMETPRLFQISALTHQ
                     GTQELVHQINQYLTEKKRLEAEKAEAEKAAANVEIIEQQPKTDTGVFKPE"
     gene            1619..2668
                     /locus_tag="ACFM8S_000744"
     CDS             1619..2668
                     /locus_tag="ACFM8S_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFC6313847.1"
                     /translation="MSSNQYIQSLELTNFTVLPNDTFEFSENLNVIVAENGCGKTHLL
                     KILYSLLEVTSNTKNRLLKTELQKSFADKLLNVFRPDSLGRLSKRLQGRGRTEIVLKL
                     QNGTTHSSLNFSSNSSSQVNVQSIGLKENEYTPTPIFLPSRELITLCPWFTSLYQNQS
                     IPFEETWFDTCMQLNHPLAKGPRETKIRELLEPIENAMGGKVSEEQGRFYLSLTNTGG
                     KIEAPLVAEGLRKFVMIARLIATGALLDKGYLFWDEPEANLNPKLIKVAARIIWSLSQ
                     QGIQVFIATHSLFLLRELELLKLENNTDLPVRFFSLIASDDGTKVEQGNSINDLNTLI
                     LLDENIMQADRYLNI"
     gene            3356..4777
                     /gene="cysS"
                     /locus_tag="ACFM8S_000745"
     CDS             3356..4777
                     /gene="cysS"
                     /locus_tag="ACFM8S_000745"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFC6313848.1"
                     /translation="MTTIYNTLTRQKEPFAPIDPKNVRMYVCGMTVYDYCHLGHARVM
                     VVFDMIARWLRECGYPLTYVRNITDIDDKIIARAAENGETIGELTARFIQAMHEDADA
                     LGVLRPDIEPKATENIPQMIAMIETLIQNGKAYPAANGDVYYAVREFAAYGQLSGKSL
                     DDLRAGERVEVDGFKRDPLDFVLWKAAKAGEPAWESPWGNGRPGWHIECSAMSENLFG
                     DTFDIHGGGADLQFPHHENEIAQSVGATGHTCGHHHAQTHHGQSIASHVKYWLHNGFI
                     RVDGEKMSKSLGNFFTIREVLKQYDPEVVRFFILRAHYRSPLNYSDAHLDDAKGALTR
                     LYTTLKNTPPADPMPSEAGDDYTRRFYVAMNDDFDTVKAVAVLFELAGEVNKTNDAQL
                     AGCLKALGGIIGLLQRDPTEFLQGGAASDGLSNEEIEDLIARRKQARSDKNWAESDRI
                     RDLLNEHKIILEDNAGGTTWRRG"
     gene            4865..5644
                     /locus_tag="ACFM8S_000746"
     CDS             4865..5644
                     /locus_tag="ACFM8S_000746"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFC6313849.1"
                     /translation="MLKIISANVNGIRSAYKKGFYEYIAASGADIVCVQELKAQEADL
                     SADMKNPHGMHGYWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFDR
                     LSVISLYLPSGSSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLK
                     NWKGNQKNSGFLPEEREWIGKVIHKLGWTDMWRTLYPDVPGYTWWSNRGQAYAKDVGW
                     RIDYQMVTPELAAKAVSAHVYKDEKFSDHAPLVMEYDYAAE"
     gene            5631..5975
                     /locus_tag="ACFM8S_000747"
     CDS             5631..5975
                     /locus_tag="ACFM8S_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313850.1"
                     /translation="MLPNKVLGKYDWNVDGKTGIGAAWVVAAFILPILVWAIFMLSRM
                     QGWLAPTKANPTWALVWLLICMPCLLIASKCLGWKGWRLLANVLACLAVCALLSVPAA
                     LLIAFTLQDLLK"
     gene            5985..6515
                     /locus_tag="ACFM8S_000748"
     CDS             5985..6515
                     /locus_tag="ACFM8S_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFC6313851.1"
                     /translation="MESFELEDLTLWLVRDADEAEMWIDRWAISYPVVQMSEASAGQS
                     IGEWQAGLQTAFERIRGKYVAVVAHGAGAAAFLAWLYQADILTRKKIANIILVPQRPD
                     IFPDDAEHTFQRVRCPCRAALVVPEHGGVPHGWAKKQADLWNVRLLLSPHSGSLNGML
                     GGWQWGMKLMQEMLLA"
     gene            complement(6744..7859)
                     /gene="asd"
                     /locus_tag="ACFM8S_000749"
     CDS             complement(6744..7859)
                     /gene="asd"
                     /locus_tag="ACFM8S_000749"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFC6313852.1"
                     /translation="MKVGFVGWRGMVGSVLMQRMKEENDFAHIPEAFFFTTSNVGGAA
                     PDFGQAAKTLLDANDVAELAKMDIIVTCQGGDYTKSVFQALRDSGWNGYWVDAASSLR
                     MKDDAIIVLDPVNRNVIDNGLKNGVKNYIGGNCTVSLMLMALGGLFQNDLVEWATSMT
                     YQAASGAGAKNMRELISGMGTIHAQVADELADPSSAILDIDRKVSDFLRSEDYPKANF
                     GVPLAGSLIPWIDVDLGNGQSKEEWKGGVETNKILGRSDNPTVIDGLCVRIGSMRCHS
                     QAITLKLKKDLPVSEIETILAGTNDWVKVIPNEKEASIHELTPAKVTGTLSVPVGRIR
                     KLGMGGEYISAFTVGDQLLWGAAEPLRRVLRIVLGSL"
     gene            8035..8616
                     /locus_tag="ACFM8S_000750"
     CDS             8035..8616
                     /locus_tag="ACFM8S_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFC6313853.1"
                     /translation="MEEKNDYTDAASDNRNGQEIEVGIAGAGDRILAALLNQLFTFLV
                     LLVPFAGLIAFAIKNEGRIGGSEEIFGLLLGMTSFWVGLAGILVYTVVQIYYMSRDGQ
                     SLGKKIMRIRVLKTDGRNPGFVGTVLVREIAWSVLVAIIAAVIGLAVGDNGENAINLL
                     AFLANFVLLFMVKRDRRTLYDILADTVVVKLPK"
     gene            complement(8678..9532)
                     /gene="folD"
                     /locus_tag="ACFM8S_000751"
     CDS             complement(8678..9532)
                     /gene="folD"
                     /locus_tag="ACFM8S_000751"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFC6313854.1"
                     /translation="MSAQLINGKEVSQKRLQAVAEAVAQRQQDNLHMPCLAVVLVGGD
                     PASAVYVRNKKTACQKCGIKSLSYELPESTSQEELLALVDRLNADSEVDGILVQLPLP
                     KHLDSQAVLERISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGK
                     KAVVVGASNIVGRPQALELLLARATVTVCHSATENLTDEVAGADILVVGVGIPNFVKG
                     EWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLMENTL
                     HAASLHDA"
     gene            9755..9832
                     /locus_tag="ACFM8S_000752"
     tRNA            9755..9832
                     /locus_tag="ACFM8S_000752"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9790..9792,aa:Pro,seq:tgg)
     gene            9857..9933
                     /locus_tag="ACFM8S_000753"
     tRNA            9857..9933
                     /locus_tag="ACFM8S_000753"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9891..9893,aa:Arg,seq:tct)
     gene            9961..10036
                     /locus_tag="ACFM8S_000754"
     tRNA            9961..10036
                     /locus_tag="ACFM8S_000754"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9994..9996,aa:His,seq:gtg)
     gene            10172..10678
                     /locus_tag="ACFM8S_000755"
     CDS             10172..10678
                     /locus_tag="ACFM8S_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HFC6313855.1"
                     /translation="MVDAWSVPDFESKICPPEALAARLALLPRPLVFTNGCFDILHRG
                     HVTYLAQARSMGGALVLALNTDASVRRLGKGGDRPINPLENRAAVAAALESVDLVTWF
                     DGDTPAALIEAVKPEILVKGGDWAADKIVGAAETLARGGQVFSIPFLHQTSTTKTLAK
                     IRAAEGGK"
     gene            10675..12453
                     /locus_tag="ACFM8S_000756"
     CDS             10675..12453
                     /locus_tag="ACFM8S_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/type III pantothenate kinase"
                     /protein_id="HFC6313856.1"
                     /translation="MTVLKPSHWRVLAELADGLPQHVSQLARMADMKPQQLNGFWQQM
                     PAHIRGLLRQHDGYWRLVRPLAVFDAEGLRELGERSGFQTALKHECASSNDEILELAR
                     IAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWVFDRPQYELGSLSPVAA
                     VACRRALSRLGLKTQIKWPNDLVVGRDKLGGILIETVRTGGKTVAVVGIGINFVLPKE
                     VENAASVQSLFQTASRRGNADAAVLLETLLAELDAVLLQYARDGFAPFVAEYQAANRD
                     HGKAVLLLRDGETVFEGTVKGVDGQGVLHLETAEGKQTVVSGEISLRSDDRPVSVPKR
                     RDSERFLLLDGGNSRLKWAWVENGTFATVGSAPYRDLSPLGAEWAEKADGNVRIVGCA
                     VCGEFKKAQVQEQLARKIEWLPSSAQALGIRNHYRHPEEHGSDRWFNALGSRRFSRNA
                     CVVVSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNRHAGKRYPFPTTT
                     GNAVASGMMDAVCGSVMMMHGRLKEKTGAGKPVDVIITGGGAAKVAEALPPAFLAENT
                     VRVADNLVIHGLLNLIAAEGGESEHT"
     gene            12566..13390
                     /locus_tag="ACFM8S_000757"
     CDS             12566..13390
                     /locus_tag="ACFM8S_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HFC6313857.1"
                     /translation="MKWLFTLLAVLNIAVFGGTVGYKLAVKATGGVPENRAVENTPPA
                     TPAADNAAASVEDTAALLKPGDILTEEQAEQLRLKKEAEQKKLREKKQREEKARREKL
                     AAEKAQAERENGAADALCAAQASLTMDEDDYHRIKGLLGKWSHVASRSVEKRTAKAKP
                     ADKTYRVVLPVSADAENQAAELSAKGFNPIPFDGALSLGVGNSRENAQALQNRLAGAG
                     FGGAHIVEHFAEADRQDDSLSVSRMTVLFTGVNAADADEIRKITSLYGKLNLKSCK"
     gene            complement(14154..14942)
                     /locus_tag="ACFM8S_000758"
     CDS             complement(14154..14942)
                     /locus_tag="ACFM8S_000758"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFC6313858.1"
                     /translation="MLTLYGETFPSRLLLGTAAYPTPEILKQSVRIAQPAMITVSLRR
                     TGSGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIG
                     DDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDAGCQALMPWAAPIGTG
                     LGAVHAYALNVLRERLPDTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVN
                     MARAFALAVESGRLAFEAGPVEARDKAQASTPTVGQPFWHSAEY"
BASE COUNT         3584 a         3774 c         4178 g         3418 t
ORIGIN      
        1 gtccacagga caatggtggt cagaatgccg atgaaaccgg caacggtcag ccagtgcagc
       61 gcgcgtgtca acgggtcgta aacggcaggt tggtttgtgt tcataaaaaa cttattatat
      121 gtaacggttt gaacggacat tccgtcgggt cggaatgtca aaaaggcgcg attgtaccaa
      181 agaagttgga cataattttg tttgcaggct gaagatttgc ttaaaaattc attaagatgg
      241 gcggaacaaa tagtttgggt acggcgtgtt gaaataccgc ttatccctta taatagcgtc
      301 cgaaattctg ttcagacggc atcaaaacac acggtaatcc gtctgaaccc cccatttgaa
      361 accaacaaac aagagcattg aatgaaattc atcgacgaag caaaaatcga agtcgccgca
      421 ggcaaaggcg gtaatggcgc aaccagtttc cgccgcgaaa aattcgtacc gcgcggcggc
      481 ccggacggcg gcgacggcgg caaaggcggt agcgtctggg cggaagccga cgaaaacacc
      541 aacaccctcg tcgaataccg cttcgtcaaa cgataccaag ccaaaaacgg cgaaaaaggc
      601 cacggttccg accgttacgg cgcaggtgcg gacgacatcg tcctcaaaat gcccgtcggc
      661 accctcatcc gcgacctcga caccgacgaa atcgttgccg acctcactta ccacggacag
      721 cgcgtctgcc tagccaaagg cggcaaaggc ggcttgggca acatccactt caaatcctcc
      781 gtcaaccgcg ccccaaaaca atccacgccc ggcgaagaag gcgaaacccg ctccctgcaa
      841 ctcgaactca aagtcctcgc cgatgtcggc ttattgggta tgcccaacgc cggcaaatcc
      901 accctgatta ccgccgtatc cgccgcacgc cccaaaatcg ccaactaccc cttcaccacc
      961 ctgcatccga acttgggcgt ggtacgcatc gacgaaaacc acagcttcgt gatggccgac
     1021 atccccggcc tgattgaagg tgcggcagaa ggcgcaggcc tcggccatcg tttcctcaaa
     1081 cacctatcgc gtaccggcct gctgttgcac gttgtcgatt tggcgccctt cgacgaagcc
     1141 gtcgatcccg ccgaagaagc ccttgccatc atcaacgaat tacgcaaata cgatgaagaa
     1201 ctctatggca aaccgcgctg gctggtgctg aacaaactcg acatgctcga tgaagaagaa
     1261 gcccaagaac gcaccgtcac cttcctcgaa gccgtcggct gggattaccc gaaaccggac
     1321 gaccgcttcc aattcgatat ggaaaccccg cgcctcttcc aaatcagcgc gctaacccac
     1381 caaggcacgc aggaattggt acaccaaatc aaccaatacc tgaccgagaa aaaacgcttg
     1441 gaagccgaaa aagcagaagc ggagaaagcg gcggcaaatg ttgaaattat cgaacagcag
     1501 cctaaaacgg atacgggcgt gtttaagccg gagtgaaaaa agaaagggtt tcagacgata
     1561 tttgttctac aaaaggttgt ttgaaacaat tttaaagcaa gattgaaatg gattaaaaat
     1621 gtcatctaac caatatatcc agtcgcttga gttgacaaac tttaccgttc ttccaaacga
     1681 tacttttgaa ttttctgaaa atctcaatgt tattgttgcc gaaaacggtt gtggcaaaac
     1741 acatttgtta aaaattttat acagcctgct agaagtaaca tctaacacca aaaacagatt
     1801 actgaaaaca gaattgcaaa aaagttttgc cgataaatta ctgaatgttt tccgtcccga
     1861 ttccttgggc aggttgagca aacgtttgca ggggcgcggg cgtacggaaa tcgttttaaa
     1921 actgcaaaac ggcactacgc atagtagtct gaatttttcc agtaactcat catcgcaagt
     1981 caatgttcaa agcatcggat tgaaagaaaa tgaatatact ccgacaccta tcttcttacc
     2041 cagccgcgaa ttaattacac tttgcccttg gttcacttct ttgtatcaaa accaaagcat
     2101 ccctttcgaa gaaacttggt ttgatacctg tatgcaatta aatcacccgc ttgccaaagg
     2161 ccccagagaa acaaaaattc gagaattgct cgaacctatt gagaacgcga tgggaggaaa
     2221 agtatctgaa gaacaaggaa ggttttatct atcgctgaca aatacgggcg gtaagattga
     2281 agcacctttg gtagcagagg gtttgcggaa atttgtcatg attgcccggt taatcgcaac
     2341 aggggcattg ctggataaag gctatctatt ttgggatgaa ccggaagcta atctgaatcc
     2401 taaattgatc aaagtggctg cccgtattat ttggtcttta tctcaacagg gtattcaggt
     2461 ttttattgca acacactctc tattcttatt gagagaattg gaacttttga agcttgaaaa
     2521 taacacggat ttacctgtac gttttttctc gttgattgct tccgatgatg gaacaaaagt
     2581 ggagcaaggg aacagtatta atgatttgaa cacattgata ttgttagatg aaaacattat
     2641 gcaggcagac aggtatctga atatttgagg ttaagctatg actacaatca cagaaaagag
     2701 attaaccttt gcttttcctg aagattatta cgttaccaag tatgacgaat gggaacatta
     2761 taaaattttt cagaacagtt gtaatttacg taataaaatt gatacaaatg aaaagggaaa
     2821 gaacgggata aatcaaagtg tagatgacga taatggcagc tcaggtgtag atataatagc
     2881 ccttcatgag tcgacacttt ggctaatcga aattaaagat tattaccgac ttgggcttga
     2941 gccaaatgcc caatcaatag atgaaaaact ttctgaccta ccatatttaa tcgcaagaaa
     3001 aattcgagat tcattggcag gattggtttc tgctaaattt aaagcagaaa aacaggaaga
     3061 aaaagatttt tctcgcttgg ctttagattg taacgaaata aaaatagtcc ttcatattga
     3121 aatgcctagt ataagaagta aactttatcc ctcgtcatca gatttagcaa acttattaaa
     3181 agataagttt aaattatcag aatttacaaa aaattttgca aactgttatg cagaaccaat
     3241 tttcacaaat atcagccata ttaataatcc gcaactacgt aatgttcctt ggtccgtatc
     3301 tacaggaaca gaacagaaat tatcatccga gcaacaaaga ttaatccata atcccatgac
     3361 caccatctac aacaccctta cccgccaaaa agaacccttt gcccccatcg accctaaaaa
     3421 cgtgcgtatg tacgtttgcg gcatgactgt ttacgactat tgccacttag gccatgcccg
     3481 tgtgatggtg gtgtttgaca tgattgcccg ctggttgcgc gagtgcggtt atccgctcac
     3541 ttatgtgcgc aacatcaccg acatcgacga caaaatcatt gcccgcgcgg ctgaaaacgg
     3601 cgagactatc ggcgaactga ccgcgcgttt cattcaggct atgcacgaag atgccgatgc
     3661 tttgggtgtg ttgcgtcccg atatcgagcc gaaggcgacg gaaaacatcc cgcaaatgat
     3721 tgccatgatt gaaaccctga ttcaaaacgg caaggcatat cctgccgcaa atggcgacgt
     3781 ttactacgcc gtgcgcgagt ttgccgctta cggacaactg tcgggcaaat cgttggacga
     3841 cctgcgcgcg ggcgaacgcg tggaagtgga cggtttcaaa cgcgatccgc ttgattttgt
     3901 attgtggaaa gcggcgaaag caggcgaacc ggcgtgggaa agcccgtggg gcaacggccg
     3961 tccgggttgg cacattgaat gctctgccat gagtgaaaac ctgttcggcg ataccttcga
     4021 catccacggc ggcggcgcgg atttgcagtt tccgcaccac gaaaacgaaa ttgcccaaag
     4081 cgtcggcgcg acggggcata cctgcggtca tcatcacgcg caaacccacc acgggcaaag
     4141 catcgccagc cacgtcaaat actggctgca caacggcttt atccgcgtgg acggcgaaaa
     4201 gatgtccaaa tcgctgggca acttcttcac catccgcgaa gtgttgaaac aatacgatcc
     4261 ggaagtcgtg cgcttcttca tcctgcgcgc ccactaccgc agcccgttga actattccga
     4321 cgcgcatttg gatgatgcaa aaggcgcgtt gacgcgtttg tatacgactt tgaaaaatac
     4381 gccgcccgcc gatccgatgc cgtctgaagc cggcgatgac tacacgcgcc gcttttatgt
     4441 tgcgatgaat gatgattttg atacggtgaa agcggtggcg gtgttgttcg aacttgccgg
     4501 cgaagtgaac aaaaccaatg acgcgcaact ggccggctgc ctgaaagcct tgggcggcat
     4561 catcggactg ctgcaacgcg acccgaccga gttcctgcaa ggcggcgcgg cttcagacgg
     4621 cctctccaac gaagaaatcg aagacttgat tgcccggcgg aaacaggcgc gctccgataa
     4681 aaactgggcg gaatccgacc gcattcgcga ccttctgaac gaacacaaaa tcattttgga
     4741 agacaacgcc ggcggcacga cttggcggcg cggttaaggg gtacggtaaa gggcaggagt
     4801 gctgtctctt gccttttcta tgtttgcaga cggcattttt tattgcgaga acacatttcc
     4861 tattatgctt aaaatcattt ccgccaacgt caacggcatc cgctccgcct acaaaaaagg
     4921 tttttacgaa tacatcgccg catcgggcgc ggacattgtc tgcgtgcagg aactcaaagc
     4981 gcaggaggct gatttgtctg ccgatatgaa aaatccgcat gggatgcacg gctattggca
     5041 ttgcgccgag aagcgcggtt acagcggcgt ggcggtgtac agcaaacgca aacccgacaa
     5101 tgtgcaaatc ggtatgggca ttgaagaatt cgacagggaa gggcggtttg tgcgttgcga
     5161 tttcgacagg ttgagcgtta tttcgcttta cttgcccagc ggcagcagcg cggaagaacg
     5221 ccagcaggtg aaataccgtt tccttgatgc gttttaccct atgctcgaag cgatgaaaaa
     5281 cgaaggacgc gacattgtcg tttgcggcga ctggaacatc gcccaccaaa acatcgacct
     5341 gaaaaactgg aaaggcaatc agaaaaattc gggcttccta ccggaagaac gcgagtggat
     5401 aggcaaggtt atccacaagc tcggttggac ggatatgtgg cgcacgcttt atcccgatgt
     5461 gccgggctac acgtggtgga gcaaccgcgg gcaggcgtat gcgaaagatg tcgggtggcg
     5521 catcgattat cagatggtta cgcccgaact tgccgccaaa gccgtgtccg cacacgttta
     5581 taaagatgaa aaattttccg accatgcgcc gctggtcatg gagtatgact atgctgccga
     5641 ataaagtttt gggtaaatat gattggaatg tggacggaaa aacaggaata ggcgcagcat
     5701 gggttgtcgc tgcgtttatc ctgcccatat tggtgtgggc gatatttatg ttgtcgcgga
     5761 tgcaaggctg gcttgcgccg accaaggcaa acccgacatg ggcgttggtg tggctgctga
     5821 tctgcatgcc ctgcctgctg attgcgtcca aatgtttggg ttggaaaggc tggcggctgt
     5881 tggcaaacgt tcttgcctgc ctcgccgtgt gcgctctgtt gagtgtgcct gccgcgctgc
     5941 tgattgcgtt cacgctgcaa gacctgctca aataggcgcg gacaatggaa agtttcgagc
     6001 ttgaggattt gacgctgtgg ctggtacgcg atgcggatga agcggaaatg tggatagacc
     6061 gctgggcgat cagttatccc gtcgtgcaga tgtccgaagc gtcggccggt caaagcatag
     6121 gggaatggca ggcagggctt cagacggcat ttgaacgcat acgcggcaaa tacgtcgccg
     6181 ttgtcgcaca cggtgcgggc gcggccgcat ttttggcgtg gctgtatcag gcagacatcc
     6241 tgacacggaa gaaaattgcc aacatcatcc ttgtaccgca gcgtcccgat atttttcccg
     6301 acgatgcgga acacactttc caacgcgtcc gctgtccctg ccgtgccgca ttggttgtac
     6361 ccgaacacgg cggcgtgccg cacggttggg cgaaaaaaca ggcagatttg tggaacgtgc
     6421 gcctgttgct ttccccgcat tcgggcagtt tgaacggtat gctcggcggc tggcagtggg
     6481 gtatgaagct gatgcaggaa atgttgctgg cgtgaacgct tgccttatcg gaatttcggc
     6541 tgtagcagga agtccgcata tcgggctgtt ttcattgacg tgcaggcgca tcaggttcgg
     6601 ataaagtcgg gctgccgcat accgccggaa atggaatcct ttatttttta cgtcggcagt
     6661 gccggcaggg cgttatccgg cagggttgca aggaaaatgc cgtctgaaac aggcttcaga
     6721 cggcatttcc attcaaacag ggcttacagg ctgcccaaca cgatacgcaa tacgcggcgc
     6781 agcggttcgg ccgcgcccca caagagctgg tcgccgacgg tgaacgcgct gatgtattcg
     6841 ccgcccatgc ccagtttgcg gatgcgtccg acagggacgg acagcgtgcc ggtaactttg
     6901 gcaggagtca gctcgtggat gctggcttct ttttcgttgg ggatgacttt cacccagtca
     6961 ttcgtgcctg ccaaaatcgt ttcgatttcg gaaacaggca ggtctttttt cagcttcaga
     7021 gtgatggctt ggctgtggca gcgcatagag ccgatgcgga cgcacaagcc gtcgattaca
     7081 gtcggattgt cgctgcgacc gaggattttg ttggtttcca cgccgccttt ccattcttct
     7141 ttggactggc cgttgcccaa atccacgtca atccacggaa tcaggctgcc ggcgagcggt
     7201 acgccgaagt tggctttcgg atagtcttcg ctgcgtagga aatcggacac tttgcggtcg
     7261 atgtcgagaa tcgcgctgga aggatcggca agctcgtcag ctacttgggc gtgaatcgtg
     7321 cccatgccgc tgatgagttc gcgcatgttt ttcgcgcctg cgccggaagc ggcttggtag
     7381 gtcatgctgg ttgcccattc gaccaaatcg ttttggaaca ggccgcccaa agccatcagc
     7441 atcagggaaa cggtacagtt gccgccgatg tagtttttca cgccgttttt gaggccgttg
     7501 tcgataacgt tgcggttgac agggtcgagg acgataatcg cgtcgtcttt catgcgcaga
     7561 gaagatgccg cgtcaaccca gtagccgttc cagccgctgt cgcgcagggc ttggaacacg
     7621 gatttggtgt aatcgccgcc ctgacaggta acgatgatgt ccattttggc gagttcggca
     7681 acatcgttgg catccaacaa tgttttggct gcctgaccga aatcaggggc tgcgccgccg
     7741 acgttggaag tggtaaagaa aaacgcttca ggaatgtggg cgaagtcgtt ttcttctttc
     7801 atacgctgca tcaaaaccga accgaccata ccgcgccagc cgacgaaacc tactttcatt
     7861 gtgttgctcc ttgaggaaaa atcgttaata aaattatgta tggcgtaaag gaagcgtttt
     7921 aacgccgtat tgggaaaatg taaagttctt tatcgggatt tctttaagta ttggcgtttt
     7981 cggtgtcgtg gcggtagaat cggggtcagt taagaacaat tatcaggaac ggatatggaa
     8041 gaaaaaaacg actacaccga cgcggcatcc gacaaccgca acgggcagga aattgaagtc
     8101 ggaattgccg gcgcaggcga ccggatactt gccgcgctgc tgaaccaatt gtttaccttt
     8161 ttggttttgt tggtgccgtt tgccggactg attgctttcg ccatcaagaa tgaaggcagg
     8221 attggcggca gtgaggaaat attcgggctg cttttgggca tgacttcctt ttgggtagga
     8281 ctggcgggca ttttggttta taccgtcgtt caaatctact atatgagtcg ggacgggcag
     8341 tcattgggta agaagatcat gagaatcagg gtgttgaaaa ccgacggccg caatccgggt
     8401 tttgtcggca cggttttggt acgcgaaatc gcatggtcgg ttttggttgc cattattgcc
     8461 gccgttatcg gtcttgcagt aggtgacaac ggagaaaacg ccatcaacct gctggcattc
     8521 cttgccaact ttgtcctgct ctttatggtc aaacgcgacc gccgcacgct ttacgacata
     8581 ctggcggata cggttgtcgt caagctgccc aaataaacgg acacggcaaa atgccgtctg
     8641 aaagcctttc agacggtatt tttatcttca gaaccgctca agcatcgtgc agtgaagccg
     8701 cgtgcagggt gttttccatc aatgtggcaa tcgtcatcgg acccacgccg ccgggaacgg
     8761 gcgtaatcat cgccgcccgt tcttttgccg tttcaaattc cacgtcgccg cacaggctgc
     8821 catcgtccaa acggttgatg cccacatcaa taacgaccgc gccaggtttg atccattcgc
     8881 ctttgacaaa gttcggaatg cctacgccga ccaccaaaat atcggctccg gcaacctcgt
     8941 ctgtcagatt ttcggttgcg ctgtggcaga ccgttaccgt tgcgcgcgcc agcagcagtt
     9001 ccaaagcctg cgggcggccg acgatattcg acgcgccgac cacgaccgct tttttcccct
     9061 tcggatcaat gccgtaagct tccaaaagcg tcatcacgcc cttgggcgta cacgggcgca
     9121 tcagcggcat tttgaccgcc agcctgccga cattgtaagg atggaagccg tccacgtcct
     9181 tatccggcga aatacgttcc aaaaccgcct ggctgtcgag gtgcttcggc agcggtagct
     9241 gaaccagaat accgtccact tcggaatcgg cattcaggcg gtcgaccagt gccagcagtt
     9301 cttcctgcga tgttgattcg ggcagctcgt aagacagtga tttgatgccg catttttggc
     9361 aggcagtttt cttgttgcgg acataaaccg cgctggcagg gtcgcctccg accaaaacca
     9421 cggccaggca aggcatgtgc agattgtcct gttggcgttg cgccaccgct tcggcaaccg
     9481 cctgcaggcg tttttgcgaa acttctttac cattgatcag ttgtgccgac atatccgtcc
     9541 ttttgttaaa caggtagaaa atgctgaaaa ttatcgcatt ttccgtttgt ttttacagca
     9601 ggatgccgaa aataaatttg ccctgaaata aaatacggct gtttggacgg attaaatatt
     9661 ttttcaaatc aaaaaactat atttttcatc ttggtgtctt gaaaataaat attgacgcat
     9721 ctttccctga gttgtatagt tcgcttcttc aagtcggggc gtagcgcagc ccggttagcg
     9781 catctgcttt gggagcagag ggtcgtgagt tcgaatccca ccgccccgac caatcaatca
     9841 gcacgaatcg gcatgagcgc ccgtagctca accggataga gcaccgacct tctaagtcgg
     9901 gggttacagg ttcgattcct gtcgggcgtg ccaagaattt gattgaaaac cgtttttatg
     9961 gtggctgtag ctcagttggt agagccccgg attgtgattc cggttgtcgt gggttcgagc
    10021 cccatcagcc accccagata aaggcccgta cttttcggta cgggtctttt cccgtttgcg
    10081 cgtactgtcc gcgccgccgg ctttgccttt cgcatatgct tctgtatcat gtgggttttt
    10141 ctgtctttta tggtttttgt cggaggcttt gatggttgac gcttggtctg tccctgattt
    10201 tgaatcgaaa atctgtccgc ccgaggcgtt ggcggcgcgt ttggcgttgt tgccgcgccc
    10261 gctggtgttt accaacggct gtttcgacat cctccacagg gggcacgtta cttatctggc
    10321 gcaggcgcgt tcgatggggg gcgcgttggt gctggcgttg aataccgatg cttcggtgcg
    10381 gcgtttgggc aagggcggcg accgcccgat taatcctttg gagaaccgtg ccgccgttgc
    10441 cgccgctttg gaaagtgtgg atttggtaac gtggtttgac ggggatacgc cggcggcgtt
    10501 gattgaggcg gtcaaacctg agattttggt caagggcggc gattgggctg cggataagat
    10561 tgtcggtgcg gcagaaacgt tggcgcgcgg cggtcaggtg ttttcaattc cgtttctgca
    10621 ccagacttcg acaacgaaga ctttggcaaa aatccgtgcg gcagagggcg gaaaatgacg
    10681 gttttgaagc cttcgcactg gcgggtgttg gcggagcttg ccgacggttt gccgcaacac
    10741 gtctcgcaac tggcgcgtat ggcggatatg aagccgcagc agctcaacgg tttttggcag
    10801 cagatgccgg cgcacatacg cgggctgttg cgccaacacg acggctattg gcggctggtg
    10861 cgcccattgg cggttttcga tgccgaaggt ttgcgcgagc tgggggaaag gtcgggtttt
    10921 cagacggcat tgaagcacga gtgcgcgtcc agcaacgacg agatactgga attggcgcgg
    10981 attgcgccgg acaaggcgca caaaaccata tgtgtgaccc acctgcaaag taagggcagg
    11041 gggcggcagg ggcggaagtg gtcgcaccgt ttgggcgagt gtctgatgtt cagttttggc
    11101 tgggtgtttg accggccgca gtatgagttg ggttcgctgt cgcctgttgc ggcagtggcg
    11161 tgccggcgcg ccttgtcgcg tttgggtttg aaaacgcaaa tcaagtggcc aaacgatttg
    11221 gtcgtcggac gcgacaaatt gggcggcatt ctgattgaaa cggtcaggac gggcggcaaa
    11281 acggttgccg tggtcggtat cggcatcaat ttcgtgctgc ccaaggaagt ggaaaacgcc
    11341 gcttccgtgc aatcgctgtt tcagacggca tcgcggcggg gaaatgccga tgccgccgtg
    11401 ttgctggaaa cgctgttggc ggaacttgat gcggtgttgt tgcaatatgc gcgggacgga
    11461 tttgcgcctt ttgtggcgga atatcaggct gccaaccgcg accacggcaa ggcggtattg
    11521 ctgttgcgcg acggcgaaac cgtgttcgaa ggcacggtta aaggcgtgga cggacaaggc
    11581 gttctgcact tggaaacggc agagggcaaa cagacggtcg tcagcggcga aatcagcctg
    11641 cggtccgacg acaggccggt ttccgtgccg aagcggcggg attcggaacg ttttctgctg
    11701 ttggacggcg gcaacagccg gctcaagtgg gcgtgggtgg aaaacggcac gttcgcaacc
    11761 gtcggtagcg cgccgtaccg cgatttgtcg cctttgggcg cggagtgggc ggaaaaggcg
    11821 gatggaaatg tccgcatcgt cggttgcgcc gtgtgcggag aattcaaaaa ggcacaagtg
    11881 caggaacagc tcgcccgaaa aatcgagtgg ctgccgtctt ccgcacaggc tttgggcata
    11941 cgcaaccact accgccaccc cgaagaacac ggttccgacc gctggttcaa cgccttgggc
    12001 agccgccgct tcagccgcaa cgcctgcgtc gtcgtcagtt gcggcacggc ggtaacggtt
    12061 gacgcgctca ccgatgacgg acattatctc gggggaacca tcatgcccgg tttccacctg
    12121 atgaaagaat cgctcgccgt ccgaaccgcc aacctcaacc ggcacgccgg taagcgttat
    12181 cctttcccga ccacaacggg caatgccgtc gccagcggca tgatggatgc ggtttgcggc
    12241 tcggttatga tgatgcacgg gcgtttgaaa gaaaaaaccg gggcgggcaa gcctgtcgat
    12301 gtcatcatta ccggcggcgg cgcggcaaaa gttgccgaag ccctgccgcc tgcatttttg
    12361 gcggaaaata ccgtgcgcgt ggcggacaac ctcgtcattc acgggctgct gaacctgatt
    12421 gccgccgaag gcggggaatc ggaacatact taaaaaatac accctttttc aacacggggc
    12481 ggcatccgtc cgaatccgtc cggattctgc cgcccgacag actgccgccc aggcgggcgg
    12541 ggtttcaaac attgaggaaa ttctaatgaa atggctcttc acccttttgg cggtactcaa
    12601 tattgccgta ttcggcggca cggtgggcta taaattagcc gttaaagcga cgggcggcgt
    12661 gccggaaaac cgggccgtcg aaaacacgcc ccccgcaacg cccgccgccg acaatgcggc
    12721 ggcatccgtg gaggatacgg cggcactgct caaaccgggc gacatcctga ccgaagagca
    12781 ggccgagcag ttgcgcttga aaaaggaagc ggaacagaaa aaactgaggg agaaaaaaca
    12841 gcgtgaagaa aaagcccgcc gcgaaaaact cgccgcagaa aaggcgcagg cggaacgcga
    12901 aaacggcgcg gcggatgcct tatgcgccgc gcaggcaagc ctcacgatgg acgaagatga
    12961 ctaccaccgc atcaaaggac ttttgggcaa atggtcgcac gttgccagca ggagcgtcga
    13021 aaaacgcacc gccaaagcca aacctgccga caaaacctat cgcgtcgtcc tgcccgtttc
    13081 cgccgatgcc gaaaatcagg cggcggagct gtctgccaaa ggtttcaacc ccataccgtt
    13141 tgacggcgca ttgagtttgg gtgtcggcaa cagcagggaa aacgcccaag ccctgcaaaa
    13201 ccggcttgcc ggcgcaggat tcggcggggc gcatattgtc gaacactttg ccgaagccga
    13261 caggcaggac gattctttga gcgtgtcgcg tatgacggtt ttgtttaccg gcgtgaatgc
    13321 cgccgatgcg gacgaaattc gtaaaatcac gtccctatac ggcaaactga acctcaagtc
    13381 ttgcaaatag gcgcgaaagc cggacgataa acgccaaacc caccgcgaag gtgggtttgg
    13441 cgtttcggtt ttgagtatct gatatataaa aagggaatgt atgtctcgta tatgccgact
    13501 ccaagtgtga aagtgaattg tgagggaata cagtgtttaa tggttcggaa tggacgactt
    13561 ccgagtggtt ttgattatac gctaataata ataattatca ataaaaattt aaaaataaat
    13621 gcggattttt tgtaatgaga tgaaaaataa acaaattaaa ctatttgccg atgtttgtga
    13681 ttcgggtcgg caaactttca ttgtgccgga caaatcgggg tcggcaggca tagctgaggg
    13741 cttcgtgcct gacggaaata aggtcgcccg tgtttgaaca ttcgtccgat ttggcgcgga
    13801 cagggttgtt atttgggggt gtttgagcgt ggattcgggg atgcgaaagc ggtaaaaccg
    13861 ttgcggatga aggcttacgg acggctttag gtatttttaa gtgtattcaa acaacaacaa
    13921 aacgcttctt ctattgccgt atttagggct tgtgcggcag cctgcacacc gtggaggccg
    13981 agtgtttcgt tggttgcggc atcgggacgg gcggcaaggt tttccggccg gtttttccat
    14041 tttttccgac aaagccgtga tgccggggaa tggcggtttg tccgtttcag gccgccgccg
    14101 ttttgggctg cgatgccgtc tgaaccgttc agacggcatt tttgccgcct ttttcaatat
    14161 tccgccgaat gccaaaacgg ctgtccgact gtcggcgtgc tggcttgcgc tttgtcgcgt
    14221 gcttcgaccg gtccggcttc aaatgccagc cgtccggatt cgacggcgag tgcgaaggcg
    14281 cgcgccatat tgaccggatc gccgctgcgg gaaacggcgg tgttcaaaag cacgccgtca
    14341 aagccccatt ccatcacttg tgccgcctgt gagggcaaac ccaagcccgc gtcgataatc
    14401 agcggcgtgt cgggcaggcg ttcgcgcagg acgttcaacg cgtaggcgtg aaccgcgccc
    14461 aaacccgtgc cgatcggggc cgcccacggc atcaacgcct gacagcccgc gtcgagcagg
    14521 cggcggcagg caatcaggtc ttcggtgcaa taaggcagca ctttgaagcc gtctttaatc
    14581 aggatttccg ccgcttcgac aagttggaac acatccggct gcaaggtgtc gtcgtcgccg
    14641 atgagttcga gtttaatcca atcggtttca aacacttcgc gcgccatttg cgccgtcgtt
    14701 accgcttcct gcacgctttg gcagcctgcc gtgttcggca ggacgggaac gccggtttct
    14761 tgaagcagcg accaaaaccc ctgaccgtgc gcctcgccgc cgcttcccgt gcggcgcagc
    14821 gagacggtaa tcatcgcggg ttgggcgata cggacggatt gtttgaggat ttcaggggtc
    14881 gggtaggcag ccgtgccgag cagcagccgt gaagggaaag tttctccgta tagggtgagc
    14941 ataatgggtt cctt
//
LOCUS       DAXHSF010000031        14932 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.031, whole genome shotgun sequence.
ACCESSION   DAXHSF010000031 DAXHSF010000000
VERSION     DAXHSF010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14932
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.031"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            126..1535
                     /gene="murC"
                     /locus_tag="ACFM8S_000759"
     CDS             126..1535
                     /gene="murC"
                     /locus_tag="ACFM8S_000759"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFC6313859.1"
                     /translation="MMKNRVTNIHFVGIGGVGMSGIAEVLHNLGFKVSGSDQARNAAT
                     EHLGSLGIQVYPGHTAEHVNGADVVVTSTAVKKENPEVVAALEQQIPVIPRALMLAEL
                     MRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKLNAAGTNARLGKGEYIVA
                     EADESDASFLHLTPIMSVVTNIDEDHMDTYGHSVEKLHQAFIDFIHRMPFYGKAFLCI
                     DSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHVQMKGHEQGSFEVV
                     LNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYGDIKLPNGGTAL
                     LVDDYGHHPVEMAATLAAARGAYPEKRLVLAFQPHRYTRTRDLFEDFTKVLNTVDALV
                     LTEVYAAGEEPIAAADCRALARAIRVLGKLEPIYCENVADLPEMLLNVLQDGDIVLNM
                     GAGSINRVPAALLELSKQI"
     gene            1648..2562
                     /locus_tag="ACFM8S_000760"
     CDS             1648..2562
                     /locus_tag="ACFM8S_000760"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFC6313860.1"
                     /translation="MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDP
                     KETPLSELKAQGFQTAFNILHGTYGEDGAVQGALELLGIPYTGSGVAASAIGMDKYRC
                     KLIWQALGLPVPEFAVLHDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKGKGRLKS
                     VYEELKHLQGEIIAERFIGGGEYSCPVLNGKGLPGIHIIPATEFYDYEAKYNRDDTIY
                     QCPSEDLTEAEESLMRELAVRGAQAIGAEGCVRVDFLKDTDGKLYLLEINTLPGMTGH
                     SLVPKSAAVTGVGFADLCIEILKTAHVG"
     gene            2552..3280
                     /locus_tag="ACFM8S_000761"
     CDS             2552..3280
                     /locus_tag="ACFM8S_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFC6313861.1"
                     /translation="MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSL
                     KGNLVYSDKKALGSLAKEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEVV
                     LTERKPVARWGDHALVDGEGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAK
                     QGLGIKEMTYTARSAWIVVLDNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYV
                     DMRYKDGFSVRYASDGLPEKESEE"
     gene            3366..4610
                     /gene="ftsA"
                     /locus_tag="ACFM8S_000762"
     CDS             3366..4610
                     /gene="ftsA"
                     /locus_tag="ACFM8S_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFC6313862.1"
                     /translation="MEQQQRYISVLDIGTSKVLALIGEVQDDDKINIVGLGQAPSRGL
                     RAGMVTNIDATVQAIRQAVNDAELMADTKITHVTTGIAGNHIRSLNSQGVVKIKDGEV
                     TQADIDRAIETAKAINIPPDQKILDAVVQDYIIDTQLGVREPIGMSGVRLDTRVHIIT
                     GASTAVQNVQKCIERCGLKSDQIMLQPLASGQAVLTEDEKDLGVCVIDIGGGTTDIAV
                     YMNGAIRHTSVIPAGGNLITKDLSKSLRTPLDAAEYIKIHYGVASCDTEGLGEMIEVP
                     GVGDRTSRQVSSKVLAAIISARIQEIFGVVLGELQKSGFPKEVLNAGIVLTGGVSMMT
                     GIVEFAEKIFDLPVRTGAPQEMGGLSDRVRTPRFSTAIGLLHAACKLEGNLPQPENGA
                     VQEREVGGGLLARLKRWIENSF"
     gene            4729..5907
                     /gene="ftsZ"
                     /locus_tag="ACFM8S_000763"
     CDS             4729..5907
                     /gene="ftsZ"
                     /locus_tag="ACFM8S_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFC6313863.1"
                     /translation="MEFVYDVAESAVSPAVIKVIGLGGGGCNAINNMVANNVRGVEFI
                     SANTDAQSLAKNHAAKRIQLGTNLTRGLGAGANPDIGRAAAQEDREAIEEAIRGANML
                     FITTGMGGGTGTGSAPVVAEIAKSLGILTVAVVTRPFAYEGKRVHVAQAGLEQLKEHV
                     DSLIIIPNDKLMTALGEDVTMREAFRAADNVLRDAVAGISEVVTCPSEIINLDFADVK
                     TVMSNRGIAMMGSGYAQGIDRARMATDQAISSPLLDDVTLDGARGVLVNITTAPGCLK
                     MSELSEVMKIVNQSAHPDLECKFGAAEDETMSEDAIRITIIATGLKEKGAVDFVPARE
                     VEAVAPSKQEQSHNVEGMIRTNRGIRTMNLTAADFDNQSVLDDFEIPAILRRQHNSDK
                     "
     gene            6046..7356
                     /locus_tag="ACFM8S_000764"
     CDS             6046..7356
                     /locus_tag="ACFM8S_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFC6313864.1"
                     /translation="MDTSKQTLLDGIFKLKANGTTVRTELMAGLTTFLTMCYIVIVNP
                     LILGETGMDMGAVFVATCIASAIGCFVMGFVGNYPIALAPGMGLNAYFTFAVVKGMGV
                     PWQVALGAVFISGLIFILFSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGI
                     IVANPATLVGLGDIHQPSALLALFGFAMVVVLGHFRVQGAIIITILTITVIASLMGLN
                     EFHGVVGEVPSIAPTFMQMDFEGLFTVSMVSVIFVFFLVDLFDSTGTLVGISHRAGLL
                     VDGKLPRLKRALLADSTAIVAGAALGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLML
                     ACLMFSPLAKSVPAFATAPALLYVGTQMLRSARDIDWDDMTEAAPAFLTIVFMPFTYS
                     IADGIAFGFISYAVVKLLCRRTKDVPPMVWIVAVLWALKFWYLG"
     gene            7982..8857
                     /gene="prpB"
                     /locus_tag="ACFM8S_000765"
     CDS             7982..8857
                     /gene="prpB"
                     /locus_tag="ACFM8S_000765"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFC6313865.1"
                     /translation="MSQHSAGARFRQAVKESNPLAVAGCVNAYFARLATQSGFKAIYL
                     SGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDTPLLVDIDVGWGGAFNIARTIRN
                     FERAGVAAVHIEDQVAQKRCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDA
                     LAVEGLDAAIERAQACVEAGADMIFPEAMTDLNMYRQFADAVKVPVLANITEFGSTPL
                     YTQSELAENGVSLVLYPLSSFRAASKAALNVYEAIMRDGTQAAVVDSMQTRAELYEHL
                     NYHAFEQKLDKLFQK"
     gene            8943..10097
                     /gene="prpC"
                     /locus_tag="ACFM8S_000766"
     CDS             8943..10097
                     /gene="prpC"
                     /locus_tag="ACFM8S_000766"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFC6313866.1"
                     /translation="MTETTQTPTFKPKKSVALSGVAAGNTELCTVGRTGNDLSYRGYD
                     ILDLAQKCEFEEVAHLLIHGHLPNKFELAAYKTKLKSMRGLPIRVIKVLESLPAHTHP
                     MDVMRTGVSMLGCVHPERESHPESEARDIADKLIASLGSILLYWYQYSHNGKRIEVES
                     DEETIGGHFLQLLHGKRPSESHIKAMHVSLILYAEHEFNASTFTARVIAGTGSDMYSS
                     ITGAIGALKGPKHGGANEVAYDIQKRYRNADEAEADIRERIGRKEIVIGFGHPVYTIS
                     DPRNVVIKEVARGLSKETGDMRLFDIAERLESVMWEEKKMFPNLDWFSAVSYQKLGVP
                     TAMFTPLFVISRTTGWSAHVLEQRKDGKIIRPSANYTGPEDLAFVEIEER"
     gene            10190..10912
                     /locus_tag="ACFM8S_000767"
     CDS             10190..10912
                     /locus_tag="ACFM8S_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFC6313867.1"
                     /translation="MDKDKGSYAGIPVQNTKELVWQKIQDIPISECGEPLVSVGNTGR
                     IRVNPVYFSQGIEGAINSVLLRRSVADRLTQVVSLLPEVYGLEIFDGWRPVIVQRALR
                     ENMKQEIEKRHPEYDEVQVREALDCFAADPDRAGMPLPHLTGGSVDVGLFDIKSGKSL
                     DMGSEFDATGHLSYSDALEKEGEYFSSARNLRRILIHAMKEAGFSNLPTEWWHFDYGN
                     QNWAYFTQQSNAVFGAVEEQDT"
     gene            10938..12143
                     /locus_tag="ACFM8S_000768"
     CDS             10938..12143
                     /locus_tag="ACFM8S_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HFC6313868.1"
                     /translation="MQTLISADNHLLLWAFVMLAAAAAIFIEQNSRLAGKVPGAVLAL
                     LIALIASNLGVIPTDAPVFDAVWSYIVPLAIPLLLFQLDLHALFKESGRMLFIFLISS
                     LGTVIGTLVSFALLKNYIPELDKVAGMISASYTGGGVNFAAMSAKLNPAKDVIAATIV
                     ADNLMMACYFFILIGISSSYWVRKVWGSPYQDKLEQEYHDPSVNKAAEYWKPKSISLQ
                     SIALSLAASILLVAVSFSLSSFLQNHFQSVDGVALKMLTGLISDKYLLLTTFTLAVVL
                     FFRKGIQKLDGSQELGTYCIYLFFVVIGVPASIPLIIKTAPLLFVFTLIIAVLNLLLT
                     LVLGKLFKFSIEEIVLACNANIGGPTTAAALAISKGWRDLVGAILLIGTVGYIIGNYI
                     GSFVYALLS"
     gene            12246..14852
                     /gene="acnD"
                     /locus_tag="ACFM8S_000769"
     CDS             12246..14852
                     /gene="acnD"
                     /locus_tag="ACFM8S_000769"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047456 - 2-methylisocitrate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HFC6313869.1"
                     /translation="MAANQRYRKPLPGTDLEYYDARAACEDIKPGSYDKLPYTSRILA
                     ENLVNRADKVDLPTLQSWLGQLIEGKQEIDFPWYPARVVCHDILGQTALVDLAGLRDA
                     IAEKGGDPAKVNPVVQTQLIVDHSLAVECGGYDPDAFRKNRQIEDRRNEDRFHFINWT
                     KTAFENVDVIPAGNGIMHQINLEKMSPVVQVKNGVAFPDTCVGTDSHTPHVDSLGVIS
                     VGVGGLEAETVMLGRASMMRLPDIVGVELNGKRQAGITATDIVLALTEFLRKERVVGA
                     FVEFFGEGARSLSIGDRATISNMTPEFGATAAMFAIDEQTIDYLKLTGRDDAQVKLVE
                     TYAKTAGLWADALKTAVYPRVLKFDLSSVTRNMAGPSNPHARFATADLAAKGLAKPYE
                     EPSDGQMPDGSVIIAAITSCTNTSNPRNVVAAALLARNANRLGLKRKPWVKSSFAPGS
                     KVAEIYLKEAGLLPEMEKLGFGIVAFACTTCNGMSGALDPKIQKEIIDRDLYATAVLS
                     GNRNFDGRIHPYAKQAFLASPPLVVAYALAGSIRFDIENDVLGIADGKEIRLKDIWPT
                     DEEIDAIVAEYVKPQQFRDVYIPMFDTGTAQKAPSPLYDWRPMSTYIRRPPYWEGALA
                     GERTLRGMRPLAILPDNITTDHLSPSNAILAVSAAGEYLAKMGLPEEDFNSYATHRGD
                     HLTAQRATFANPKLFNEMVKNEDGSVCQGSLARVEPEGETMRMWEAIETYMNRKQPLI
                     IIAGADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNLIGMGVLPLQFKPDTNRH
                     TLQLDGTETYDVVGERTPRCDLTLVIHRKNGETVEVPVTCRLDTAEEVLVYEAGGVLQ
                     RFAQDFLEGNAA"
BASE COUNT         3689 a         3621 c         3938 g         3684 t
ORIGIN      
        1 attttgcaaa ggtctcatcc tgtatgccga aacatctgta atttacaaga aaactatggc
       61 gtagaagcgg atagcccttt aaaataacgc ctttacgcat cgaaaatcca ccggaacgca
      121 acattatgat gaaaaatcga gttaccaaca tccattttgt cggtatcggc ggcgtcggca
      181 tgagcggtat cgccgaagtc ttgcacaatt tgggttttaa agtttccggt tcggatcagg
      241 cgcgaaatgc cgctaccgag catttgggca gcctgggcat tcaagtttat cccggccata
      301 ccgcagaaca cgttaacggc gcggatgtcg tcgttacctc taccgccgtc aaaaaagaaa
      361 atcccgaagt tgtcgctgcg ttggagcagc aaattcccgt tattccgcgc gccctgatgt
      421 tggcggagtt gatgcgcttc cgtgacggca tcgccattgc cggcacgcac ggcaaaacca
      481 cgaccaccag cctgaccgcc tccatcctcg gcgcggcagg acttgacccg actttcgtta
      541 tcggcggcaa actcaacgcc gcaggcacca acgcccgctt gggcaaaggc gaatacatcg
      601 ttgccgaagc cgacgagtcg gatgcatcct ttctgcacct gacaccgatt atgtccgtcg
      661 ttaccaatat cgacgaagac catatggata cctacgggca cagcgtcgaa aaactgcatc
      721 aggcgtttat cgatttcatc caccgtatgc ccttctacgg caaagccttt ttgtgtattg
      781 acagcgaaca cgtccgcgcg attttgccca aagtgagcaa accttatgct acttacggtt
      841 tggacgatac cgccgacatc tacgccaccg acatcgaaaa cgtcggcgcg caaatgaaat
      901 tcaccgtcca tgttcaaatg aaaggacatg agcaggggtc gtttgaagtc gtgctgaata
      961 tgcccggcag acacaacgtg ctgaacgcat tggcagccat cggcgtggcg ctggaagtcg
     1021 gcgcatcggt tgaagcgatc caaaaaggct tgctcggctt tgaaggcgtc ggccgccgct
     1081 tccaaaaata cggcgacatc aagttgccaa acggcgggac cgcgctcttg gtggacgact
     1141 acggacacca ccccgtcgaa atggcggcga cccttgccgc cgcacgcggc gcgtatccgg
     1201 aaaaacgttt ggtactcgcc ttccagccgc accgctatac ccgcacgcgc gatttgtttg
     1261 aagactttac caaagtcctc aataccgttg acgcgctggt gctgaccgaa gtttatgccg
     1321 ccggtgaaga gccgattgcc gccgccgatt gccgcgctct tgcccgcgcc atccgcgtgt
     1381 tgggcaaact cgagccgatt tactgcgaaa acgttgccga tctgcccgaa atgctgttga
     1441 acgttttgca ggacggcgac atcgtgttga atatgggcgc gggaagcatc aaccgcgtcc
     1501 ccgccgcgct gctggaattg tcgaaacaga tttgaggcac acccgcctga cagacggaac
     1561 atcatataaa ggtcgtctga aaccgcaaat caggtttcag acgacctctg gcaacaagca
     1621 taaagcaatc aggaaagaac aaaaacaatg cagaattttg gcaaagtggc cgtattgatg
     1681 ggcggttttt ccagcgaacg agaaatctcg ctggacagcg gcaccgccat tttgaatgct
     1741 ttaaaaagca aaggcataga cgcatacgcc ttcgatccta aagaaacccc attgtctgaa
     1801 ttgaaggcac aaggttttca gacggcattc aacatccttc acggtactta cggcgaagac
     1861 ggggcggttc agggtgcatt ggaactgttg ggcattccct ataccggcag cggtgtcgcc
     1921 gcatccgcca tcggcatgga caaataccgc tgcaaactga tttggcaggc attgggattg
     1981 cccgttcccg agttcgccgt cctgcacgac gacactgatt tcgatgccgt cgaagaaaaa
     2041 ttgggcctgc cgatgtttgt gaaaccggcg gccgaaggca gcagcgtagg cgtggtaaaa
     2101 gtcaaaggaa aaggccgtct gaaaagcgtt tacgaagaat tgaaacacct tcagggcgaa
     2161 atcattgccg aacgttttat cggcggcggc gaatattcct gccccgtcct gaacggcaaa
     2221 gggctgcccg gcatacacat cattcccgca accgagtttt acgactacga agccaagtac
     2281 aaccgcgacg acaccattta tcaatgtcct tcggaagatt tgaccgaagc cgaagaaagc
     2341 ctgatgcgcg aactggcggt tcgcggcgcg caggcaatcg gtgcggaagg ctgcgtgcgc
     2401 gtcgatttcc tcaaagatac cgacggcaaa ctctatctgt tggaaatcaa caccctgccc
     2461 ggtatgaccg gccatagttt agtaccgaaa tccgctgccg ttacgggcgt gggttttgcc
     2521 gatttatgta ttgaaatttt gaagaccgca catgtgggat aatgccgaag cgatggaacg
     2581 gctgacgcgc tggctgcttg tcatgatggc gatgctgctt gctgcgtccg ggctggtttg
     2641 gttttacaat tcgaatcatc tgcccgtcaa gcaggtgtcg ctgaagggca acctggttta
     2701 ttccgataag aaagcattgg gcagtttggc gaaagaatac atccatggga atattttgag
     2761 gacggacatc aatggcgcac aggaggccta ccgccggtat ccgtggattg cgtcggtcat
     2821 ggtgcgccgc cgttttcccg acacggttga ggtcgtcctg accgagcgca agccggtcgc
     2881 gcgttggggc gaccatgcct tggtggacgg cgaaggcaat gtttttgaag cccgcttgga
     2941 cagacccgga atgccggtat tcagaggcgc ggaaggaacg tctgccgaaa tgctccgccg
     3001 ttatgacgaa ttttcgactg ttttggcaaa acagggtttg ggcatcaaag agatgaccta
     3061 tacggcacgt tcggcgtgga ttgtcgtttt ggacaacggc atcaccgtca ggctcggacg
     3121 ggaaaacgag atgaaacgcc tccggctttt taccgaagcg tggcagcatc tgttgcgtaa
     3181 aaataaaaat cggttatcct atgtggatat gaggtataag gacggatttt cagtccgcta
     3241 tgcttccgac ggtttacccg aaaaagaatc cgaagaatag tgggaacagg tatcggacag
     3301 attacggccg tgccgtctga aacggtgcga cgcaaatttc aatcagtttt aagagcagac
     3361 gaacaatgga acagcagcaa agatacatca gcgtactgga tatcggtacg tctaaagtcc
     3421 tcgcactgat cggggaagtt caagatgacg acaaaatcaa catcgtcggt ttggggcagg
     3481 ctccttcacg gggcttgcgc gcgggcatgg taaccaatat cgatgccacc gtccaagcca
     3541 tcaggcaggc ggtcaatgat gccgagctga tggcggatac caaaattacc cacgttacca
     3601 caggtatcgc aggcaaccac atccgcagtc tcaattcgca aggtgtggtt aaaattaaag
     3661 acggggaagt cacgcaggca gacatcgacc gcgccattga aacggcaaag gcaatcaata
     3721 tcccgcccga tcaaaaaatt ctcgatgccg tggttcaaga ctacattatc gacacccaac
     3781 ttggcgtgag ggagcccatc ggtatgagcg gtgtgcgtct ggatacgcgg gtgcacatca
     3841 ttaccggtgc aagtacggca gtgcagaatg tccaaaaatg tatcgagcgg tgcggcttga
     3901 aaagcgatca aatcatgctt cagccgttgg caagcgggca ggcggtgctg actgaagatg
     3961 aaaaagacct cggcgtatgc gtcatcgaca ttggtggcgg aacgaccgat attgccgttt
     4021 atatgaacgg cgccatccgc catacgtccg tcattccggc cggtggtaat ctgattacca
     4081 aagatttgtc caaatcgttg agaacacctc tcgatgccgc cgagtacatt aaaatccatt
     4141 atggcgtggc atcatgcgat acggaaggct tgggtgagat gattgaagtt ccgggcgtgg
     4201 gtgaccggac atcgcgtcag gtttccagta aggttttggc agcaatcatc agcgcacgta
     4261 ttcaggagat ttttggcgta gtgctgggcg agctgcaaaa atcgggtttc cccaaagaag
     4321 tgctgaatgc gggtatcgtt ctgaccggcg gtgtgtccat gatgaccggg attgtggaat
     4381 ttgccgaaaa aatcttcgat ttgcctgtac gcaccggtgc gccccaagaa atgggcggtt
     4441 tgtccgaccg cgtccgcaca ccgcgctttt ctaccgccat cgggctgctt catgcagcat
     4501 gcaagctgga aggaaacttg ccgcagccgg aaaacggtgc agtgcaagag agggaagtgg
     4561 gcggcggttt gttggcaaga ttgaaacggt ggattgaaaa cagcttctga acaggtggat
     4621 tgccgtttga caggtgagaa gtattttgcc agcagcaaga tacttcttat ataatgaata
     4681 ataatttatt taaaccgtcc tctgaatggg gcgagcagga gtttttgaat ggaatttgtt
     4741 tacgacgtgg cagaatcggc agtcagccct gcggtgatta aagtaatcgg cttgggcggc
     4801 ggcggttgca atgcaatcaa taacatggtt gccaacaatg tgcgcggtgt ggagtttatc
     4861 agtgccaata cggatgcgca gtctctggca aaaaaccatg cggcgaagag aatccagttg
     4921 ggtacgaatc tgacacgcgg tttgggcgcg ggcgcgaatc ccgatatcgg ccgtgcggca
     4981 gcccaggaag accgggaagc cattgaagaa gccattcgcg gtgcgaatat gctgtttatc
     5041 acgaccggta tgggcggcgg taccggtacc ggttccgcgc cggttgttgc tgagattgcc
     5101 aagtctttgg gcattctgac cgttgccgtg gttacccgac cgttcgcata tgaaggtaag
     5161 cgcgtccatg tcgcacaggc agggttggaa cagttgaaag aacacgtcga ttcgctgatc
     5221 atcatcccga acgacaaact gatgactgca ttgggtgaag acgtaacgat gcgcgaagcc
     5281 ttccgtgccg ccgacaatgt attgcgcgat gcggtcgcag gcatttccga agtggtaact
     5341 tgcccgagcg aaatcatcaa cctcgacttt gccgacgtga aaaccgtgat gagcaaccgc
     5401 ggtatcgcta tgatgggttc gggttatgcc caaggtatcg accgtgcgcg tatggcgacc
     5461 gaccaggcca tttccagtcc gctgctggac gatgtaacct tggacggagc gcgcggtgtg
     5521 ctggtcaata ttacgactgc tccgggttgc ttgaaaatgt ccgagttgtc cgaagtcatg
     5581 aaaatcgtca accaaagcgc gcatcccgat ttggaatgca aattcggtgc ggctgaagac
     5641 gagaccatga gcgaagatgc catccggatt accattatcg ctaccggtct gaaagaaaaa
     5701 ggcgcggtcg attttgttcc ggcaagggag gtagaagcgg ttgccccgtc caaacaggag
     5761 caaagccata atgtcgaagg tatgatccgc accaatcgcg gtatccgcac gatgaacctt
     5821 accgctgcgg atttcgacaa tcagtccgta cttgacgact ttgaaatccc tgcgattttg
     5881 cgtcgtcaac acaattcaga caaataatgt gctgtttgcc cgtaaacctg ctgcctcccg
     5941 aatcggtttg tccggtttgg gaggtatgtt tttcaagatg ttgcaatttc gtacggtttg
     6001 cggtcggcgg attcagattt ttccacttga tacagacttt cagatatgga cacttcaaaa
     6061 caaacactgt tggacgggat ttttaagctg aaggcaaacg gtacgacggt gcgtaccgag
     6121 ttgatggcgg gtttgacaac ttttttgacg atgtgctaca tcgttatcgt caaccctctg
     6181 attttgggcg agaccggcat ggatatgggg gcggtattcg tcgctacctg tatcgcgtct
     6241 gccatcggct gttttgttat gggttttgtc ggcaactatc cgattgcact cgcaccgggg
     6301 atggggctga atgcctattt cacctttgcc gtcgttaagg gtatgggcgt gccttggcag
     6361 gttgcgttgg gtgcggtgtt catctccggt ctgattttca tcctgttcag cttttttaaa
     6421 gtcagggaaa tgctggtcaa cgcactgcct atgggtttga aaatgtcgat tgctgccggt
     6481 atcggtttgt ttttggcact gatttccctg aaaggcgcag gcattatcgt tgccaatccg
     6541 gcaaccttgg tcggtttggg cgatattcat cagccgtccg cgttgttggc actgttcggt
     6601 tttgctatgg tggtcgtatt gggacatttc cgcgttcaag gtgcaatcat cattaccatc
     6661 ttgaccatta ccgtcattgc cagcctgatg ggtttgaacg agtttcacgg cgtggtcggc
     6721 gaagtaccga gcattgcgcc gacttttatg cagatggatt ttgaaggcct gtttaccgtc
     6781 agcatggtca gtgtgatttt cgttttcttc ttggtcgatc tatttgacag taccggaacg
     6841 ctggtcggca tatcccaccg tgccgggctg ctggtggacg gtaagctgcc ccgcctgaaa
     6901 cgcgcactgc ttgcagactc taccgccatt gtggcaggtg cggctttggg tacttcttcc
     6961 accacgcctt atgtggaaag cgcggcgggc gtatcggcag gcggacggac cggcctgacg
     7021 gcggttaccg tcggcgtatt gatgctcgcc tgcctgatgt tttcaccttt ggcgaaaagt
     7081 gttcccgctt ttgccaccgc gcccgccctg ctttatgtcg gcacgcagat gctccgcagt
     7141 gcgagggata ttgattggga cgatatgacg gaagccgcac ccgcattcct gaccattgtc
     7201 ttcatgccgt ttacctattc gattgcagac ggcatcgcct tcggcttcat cagctatgcc
     7261 gtggttaaac ttttatgccg ccgcaccaaa gacgttccgc ctatggtatg gattgttgct
     7321 gtattgtggg cactgaaatt ctggtatttg ggctgattga ttcgatatta aaaatgccgt
     7381 ctgaaaggtt ttcagacggc attttgtttg ccggtatatt aaatttttat taaattaaat
     7441 aaaaatcaaa tacataataa aatacatcgg attgcttaaa aataatacat tgtttttatg
     7501 tataaaatat tttataagtt ttcaggattt tgattatcaa aaatttttct tgatttcctg
     7561 acaattttat tgaaacaaat aattcaaaat taatctagtt taatcatgga attaaaataa
     7621 aatattaaaa ttatgtaatg agtctcctta aaaatgtttg acattttcag tcttgtgttt
     7681 tagattagcg aaaaataaaa ctacacaata ctacaaagga acattactat gaaaccaatt
     7741 cagatgtttt ccccttttct gaataatccc cttgttttct tcttgtctgc ggttttgccg
     7801 cataattccg aacggtctgc tgtttttctt tgattcgttt taaatatcaa taagataatt
     7861 tttcccatat atttttaatg attggattgg gatgcccgac gcgtcggatg gctgtgtttt
     7921 gccgtccgaa tgtgatggaa gcctgtccat actgaaaaaa agtctataaa ggagaaatat
     7981 gatgagtcaa cactctgccg gagcacgttt ccgccaagcc gtgaaagaat cgaatccgct
     8041 tgccgtcgcc ggttgcgtca atgcttattt tgcacgattg gccacccaaa gcggtttcaa
     8101 agccatctat ctgtccggcg gcggcgtggc agcctgttct tgcggtatcc ctgatttggg
     8161 cattaccaca atggaagatg tgctgatcga cgcacgacgc attacggaca acgtggatac
     8221 gcctctgctg gtggacatcg atgtgggttg gggcggtgca ttcaatattg cccgtaccat
     8281 tcgcaacttt gaacgcgccg gtgttgcagc ggttcacatc gaagatcagg tagcgcaaaa
     8341 acgctgcggc caccgtccga acaaagccat tgtatctaaa gatgaaatgg tcgaccgtat
     8401 caaagctgcc gtagatgcgc gcgttgatga gaacttcgtg attatggcgc gtaccgatgc
     8461 gctggcggta gaaggtttgg atgccgctat cgaacgcgcc caagcttgtg tcgaagccgg
     8521 tgcggacatg attttccctg aagccatgac cgatttgaac atgtaccgcc aatttgcaga
     8581 tgcggtgaaa gtgcccgtgt tggcgaacat taccgagttt ggttccactc cgctttatac
     8641 ccaaagcgag ctggctgaaa acggcgtgtc gctggtgctg tatccgctgt catcgttccg
     8701 tgcagcaagc aaagccgctc tgaatgttta cgaagcgatt atgcgcgatg gcactcaggc
     8761 ggcggtggtg gacagtatgc aaacccgtgc cgagctgtac gagcatctga actatcatgc
     8821 tttcgagcaa aaactggata aattgtttca aaaatgattt accgctttca gacggtcttt
     8881 caacaaatcc gcatcggtcg tctgaaaacc cgaaacccat aaaaacacaa aggagaaata
     8941 ccatgactga aactactcaa accccgactt tcaaacctaa aaaatccgtt gcgctttctg
     9001 gcgttgcggc tggtaatacc gaattgtgta ccgttggccg taccggcaac gatttgagct
     9061 atcgcggtta cgacatcttg gatttggcac aaaaatgcga gtttgaagaa gtcgcccacc
     9121 tgctgattca cggtcatctg cccaacaaat tcgagctggc cgcttataaa accaagctca
     9181 aatccatgcg cggcctgcct atccgtgtga ttaaagtttt ggaaagcctg cctgcacata
     9241 cccatccgat ggacgtaatg cgtaccggcg tatccatgct gggctgcgtt catcctgaac
     9301 gtgaaagcca tccggaaagt gaagcgcgcg acatcgccga caaactgatc gccagcctcg
     9361 gcagcatcct cttgtactgg tatcaatatt cgcacaacgg caaacgcatt gaggttgaaa
     9421 gcgacgaaga gaccatcggc ggtcatttcc tgcaactgtt gcacggcaaa cgcccaagcg
     9481 aatcacacat caaagccatg cacgtttcac tgattctgta tgccgaacac gagttcaacg
     9541 cttctacctt taccgcccgc gtgatcgccg gtacaggctc tgatatgtac tccagcatta
     9601 ccggagcaat cggcgcgttg aaaggtccga aacacggcgg cgcgaacgaa gtggcttacg
     9661 atattcaaaa acgctaccgc aatgccgacg aagctgaagc cgacatccgc gaacgcatcg
     9721 gccgcaaaga aatcgtgatc ggtttcggtc atccggtgta caccatttcc gaccctcgca
     9781 acgttgtcat taaagaagtg gcacgcggtt tgagcaaaga aaccggcgat atgcgcctct
     9841 ttgacattgc cgaacgtttg gaaagcgtga tgtgggaaga gaaaaaaatg ttcccgaatc
     9901 tggactggtt ctctgccgtt tcctaccaaa aattgggcgt accgaccgct atgttcacac
     9961 cgctgttcgt gatttcccgt acaaccggtt ggagcgcaca cgttctcgag caacgcaaag
    10021 acggcaaaat catccgtccg agcgcgaact acacaggccc tgaagatttg gcgtttgtgg
    10081 agattgaaga acgctgatga ttttcagacg accttgtttg tacaaggtcg tctgaaaaaa
    10141 gaaatttaat gaatttattg gcacactaat tattaatgcg gaacatttga tggataagga
    10201 caaggggtct tatgcgggta ttcctgtaca aaataccaaa gagttagttt ggcagaaaat
    10261 acaagacata cctatttctg aatgtgggga accgttggtc agtgttggta ataccggacg
    10321 cattcgtgtc aatcctgttt atttttctca gggtattgaa ggggcgataa atagtgtttt
    10381 attgcgtaga agtgttgctg ataggctgac tcaggttgtc tctttgctgc cggaagtgta
    10441 cgggttggaa atttttgatg gttggcgtcc tgtcatagtg caaagagcat tacgagaaaa
    10501 tatgaagcaa gaaattgaaa aacgccatcc ggaatatgat gaagttcaag ttagagaagc
    10561 actggactgt tttgctgctg atccagatcg ggcaggaatg cctctgcccc atttgacagg
    10621 cggatctgtt gatgtcggat tgtttgatat aaaaagtggc aaaagtttgg atatgggaag
    10681 tgagtttgat gcaactggac atttatctta tagcgatgct ttagagaaag aaggtgagta
    10741 tttctcctct gctcgtaatt tacgccggat tttgattcat gcgatgaaag aggcaggatt
    10801 tagtaatttg ccaaccgaat ggtggcattt cgattatggc aaccaaaatt gggcttattt
    10861 tacccaacaa agcaacgcag tttttggggc ggtagaagag caggatactt aaattaaaat
    10921 aaaaattggg aggaaaaatg caaactttaa tttcagcaga taatcatttg ctgctgtggg
    10981 cttttgttat gttagctgcc gctgctgcga tttttattga gcaaaacagt cgcttggctg
    11041 ggaaagtccc tggtgcagta ttagctttat tgattgcgtt gattgcttct aatttgggtg
    11101 ttattccgac ggatgctcca gtatttgatg ctgtatggtc ttatattgta cctcttgcta
    11161 ttccgttgtt attgtttcaa ttagatcttc atgctctatt taaggaaagt ggacgtatgc
    11221 tgtttatttt cctgattagt tcactaggga cggtcattgg aacgcttgtc tcatttgcac
    11281 tattgaaaaa ctatattcct gaactagata aggtagctgg catgatttca gcatcttata
    11341 cgggaggtgg tgttaatttt gcagctatgt cagctaagct taaccctgct aaagatgtta
    11401 ttgcagcaac aattgttgct gataatttga tgatggcttg ttactttttt attttgattg
    11461 gtatctcttc ctcatattgg gtcagaaaag tttggggaag tccatatcag gataaattgg
    11521 agcaggaata tcatgatccg agtgtgaata aggctgccga gtattggaaa cctaaatcca
    11581 tttcgctaca gagtatagcc ttatctttgg ctgcctcgat tttgttggtc gcagtttcat
    11641 tctcattatc cagttttttg caaaatcatt ttcaaagtgt tgatggtgtt gcattaaaaa
    11701 tgctgacagg tttaattagt gacaaatatt tgctgttaac gacatttaca ttggcagttg
    11761 ttttattttt ccgtaaaggc attcaaaaac tagatggcag tcaagaatta gggacatatt
    11821 gtatttatct gttttttgtt gtgattggtg ttcctgcctc tataccgtta attattaaaa
    11881 cagcaccatt actctttgtt tttactttga ttattgcggt attgaatttg ttactgacgt
    11941 tagtgttagg caagctgttc aaattcagta ttgaagaaat tgtattggct tgtaatgcca
    12001 atattggagg gccgactact gcagcagcct tagccatcag taaaggttgg cgtgatttag
    12061 tcggtgcaat tttgttaatt ggtactgtag gctacattat cggtaactat ataggttcgt
    12121 ttgtatatgc cctattgagc tgaagactaa tctaacaatc catgaacctt ttcagacgac
    12181 ccccttccaa aaacgtcggc tgaaacctca aaccacaaga aaaacagatc cccaggagaa
    12241 ccgacatggc tgccaaccaa cgttaccgca aaccgctgcc cggtacggat ttggaatact
    12301 acgacgcgcg tgcggcgtgt gaggacatca agcccggctc ttacgacaag ctgccttaca
    12361 cgagccgcat tttggcggag aatttggtca accgcgcgga caaagtcgat ttgccgacgc
    12421 tgcaaagctg gctgggtcag ctgattgagg gaaaacagga aatcgacttt ccttggtatc
    12481 cggcgcgggt ggtgtgccac gatattctgg ggcagaccgc gttggtggat ttggcaggtc
    12541 tgcgcgatgc gattgccgaa aaaggcggcg atcctgccaa agtgaatccg gtggtgcaaa
    12601 cccagctcat cgtcgaccac tctctggccg ttgaatgcgg cggctacgac cccgatgcct
    12661 tccgcaaaaa ccgccaaatc gaagacagac gtaacgaaga ccgtttccac ttcatcaact
    12721 ggacaaaaac cgcatttgaa aatgtggacg tgattccggc gggcaacggc atcatgcacc
    12781 aaatcaatct agaaaaaatg tcgcccgtcg tccaagtcaa aaacggcgtg gcgttccccg
    12841 atacctgcgt cggtactgac tcacatacgc cgcacgtcga ttcattgggc gtgatttccg
    12901 tgggcgtggg cggattggaa gcggaaaccg taatgctggg acgcgcgtcc atgatgcgcc
    12961 tgcccgatat tgtcggcgtt gagctgaacg gcaaacggca ggcgggcatt acggcgacgg
    13021 atattgtgtt ggcactgacc gagtttctgc gcaaagaacg cgtggtcggg gcgtttgtcg
    13081 aattcttcgg cgagggcgcg agaagcctgt ctatcggcga ccgcgcgacc atttccaaca
    13141 tgacgccgga gttcggcgcg actgccgcga tgttcgctat tgatgagcaa accattgatt
    13201 atttgaaact gaccggacgc gacgacgcgc aggtgaaatt ggtggaaacc tacgccaaaa
    13261 ccgcaggctt atgggcagat gctttgaaaa ccgccgttta tccgcgcgtt ttgaaatttg
    13321 atttgagcag cgtaacgcgc aatatggcag gcccaagtaa cccgcatgcc cgttttgcga
    13381 ccgccgattt ggcggcgaaa gggctggcga agccttacga agagccttcg gacggccaaa
    13441 tgcccgacgg ctcggtcatc atcgccgcga ttaccagttg caccaacact tccaacccgc
    13501 gcaacgtcgt tgccgccgcg ctcttggcac gcaatgccaa ccgcctcggc ttgaaacgca
    13561 aaccttgggt gaaatcttcg tttgccccgg gttcaaaagt agccgaaatc tatttgaaag
    13621 aagcgggcct gttgcccgaa atggaaaaac tcggcttcgg tatcgttgcc ttcgcatgta
    13681 ccacctgtaa cggcatgagc ggcgcgctgg atccgaaaat ccaaaaagaa atcatcgacc
    13741 gcgatttgta cgccaccgcc gtattgtcag gcaaccgcaa cttcgacggc cgcatccacc
    13801 cgtatgcgaa acaggctttc ctcgcttcgc ctccgttggt cgttgcctac gcgctggcag
    13861 gcagcatccg tttcgatatt gaaaacgacg tactcggcat tgcagacggc aaagaaatcc
    13921 gcctgaaaga catctggcct accgacgaag aaatcgatgc catcgttgcc gaatatgtga
    13981 aaccgcaaca attccgcgac gtttatatcc cgatgttcga caccggcaca gcacaaaaag
    14041 caccaagccc gctgtacgac tggcgtccaa tgtccaccta tatccgccgt ccgccttact
    14101 gggaaggcgc gctggcaggg gaacgcacat tgagaggcat gcgtccgctg gcgattttgc
    14161 ccgacaacat caccaccgac catctctcgc catccaatgc gattttggcc gtcagtgccg
    14221 caggcgagta tttggcgaaa atgggtttgc ctgaagaaga cttcaactct tacgcaaccc
    14281 accgcggcga ccacttgacc gcccaacgcg ctaccttcgc caatccgaaa ctgtttaacg
    14341 aaatggtgaa aaacgaagac ggcagcgtgt gccaaggctc gctcgcccgc gtcgaacccg
    14401 aaggcgaaac catgcgcatg tgggaagcca tcgaaaccta tatgaaccgc aaacagccgc
    14461 tcatcatcat tgccggtgcg gactatggtc aaggctcaag ccgcgactgg gctgcaaaag
    14521 gcgtacgcct cgccggcgta gaagcgattg ttgccgaagg cttcgagcgt atccaccgca
    14581 ccaaccttat cggcatgggc gtgttgccgc tgcagttcaa acccgacacc aaccgccata
    14641 ccctgcaact ggacggtacg gaaacctacg acgtggtcgg cgaacgcaca ccgcgctgcg
    14701 acctgaccct cgtgattcac cgtaaaaacg gcgaaaccgt cgaagttccc gttacctgcc
    14761 gcctcgatac tgcagaagaa gtattggtat atgaagccgg cggcgtgttg caacggtttg
    14821 cacaggattt tttggaaggg aacgcggctt agaggtcgtc tgaaaagcaa gacgtagcgt
    14881 gggtcgggtt caacattttg ctcattcacg taattctcga tatggcaggc at
//
LOCUS       DAXHSF010000032        14880 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.032, whole genome shotgun sequence.
ACCESSION   DAXHSF010000032 DAXHSF010000000
VERSION     DAXHSF010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14880
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.032"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(104..1888)
                     /gene="lpdA"
                     /locus_tag="ACFM8S_000770"
     CDS             complement(104..1888)
                     /gene="lpdA"
                     /locus_tag="ACFM8S_000770"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC6313870.1"
                     /translation="MSLVELKVPDIGGHENVDIIAVEVNVGDTIAVDDTLITLETDKA
                     TMDVPAEVAGVVKEVKVKVGDKISEGGLIVVVEAEGTAAAPKAEAAAAPAQEAPKAAA
                     PAPQAAQFGGSADAEYDVVVLGGGPGGYSAAFAAADEGLKVAIVERYKTLGGVCLNVG
                     CIPSKALLHNAAVIDEVRHLAANGIKYPEPELDIDMLRAYKDGVVSRLTGGLAGMAKS
                     RKVDVIQGDGQFLDPHHLEVSLTAGDVYEQAAPTGEKKIVAFKNCIIAAGSRVTKLPF
                     IPEDPRIFDSSGALALKEVPGKLLIIGGGIIGLEMGTVYSTLGSRLDVVEMMDGLMQG
                     ADRDLVKVWQKQNEYRFDNIMVNTKTVAVEPKEDGVYVTFEGANAPKEPQRYDAVLVA
                     AGRAPNGKLISAEKAGVAVTDRGFIEVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHEG
                     HVAAENCAGHKAYFDARVIPGVAYTSPEVAWVGETELSAKASGRKITKASFPWAASGR
                     AIANGCDNGFTKLIFDAETGRIIGGGIVGPNGGDMIGEVCLAIEMGCDAADIGKTIHP
                     HPTLGESIGMAAEVALGTCTDLPPQKKK"
     gene            complement(1965..3557)
                     /gene="aceF"
                     /locus_tag="ACFM8S_000771"
     CDS             complement(1965..3557)
                     /gene="aceF"
                     /locus_tag="ACFM8S_000771"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFC6313871.1"
                     /translation="MSIVEIKVPDIGGHENVDIIAVEVKAGDTIAVDDTLITLETDKA
                     TMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGAAAAEAAPAAAEAQPAPAAAPVA
                     AGGATVQVAVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGVV
                     KAVFLKVGDKVSEGSAIIEVETVGSAAAAPAQAAQAAAPAAAPPPTAAAAPAAAPAAA
                     KIDEAAFAKAHAGPSARKLARELGVDLGQVKGTGLKGRIVGDDIKAFVKSVMQGGAAK
                     PAAAGASLGSGLDLLPWPKVDFSKFGNVEVKELSRIKKISGQNLSRNWVVIPHVTVHE
                     EADMTELEEFRKQLNKEWEREGVKLSPLAFIIKASVSALKAFPEFNASLDGDNLVLKN
                     YFNIGFAADTPNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQGACFTI
                     SSLGGIGGTGFTPIVNAPEVAILGVCKSQIKPVWNGKEFAPRLMCPLSLSFDHRVIDG
                     AAGMRFTVFLAKLLKDFRRITL"
     gene            complement(3707..6370)
                     /gene="aceE"
                     /locus_tag="ACFM8S_000772"
     CDS             complement(3707..6370)
                     /gene="aceE"
                     /locus_tag="ACFM8S_000772"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFC6313872.1"
                     /translation="MSTQLHDVDPIETQEWLDALSSVLEYEGGERAQYLLENLVKYCR
                     DKGVRMPHGTTTPYLNTVSVENEKGIPGDQNIEHRIRAFVRWNAAAIVLRAGKKDLEL
                     GGHIASFQSAATMYEVGFNHFWKAKGEGEEGDLVFFQGHVAPGIYARAFVEGRLTEDQ
                     LNNFRQEVDGHGLPSYPHPHLLPDFWQFPTVSMGLGPIMAIYQARFLKYLESRGLAKT
                     KGRKVWCFCGDGEMDEPESQGAIALAAREGLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEGNFAGAGWNVVKVIWGRRWDRLLAKDKDGILRQRMEECLDGDYQTYKSKDGAYVRE
                     HFFNTPELKALVADMTDEQLWALNRGGHDPQKVYNAYDRAANHADGKPTVILAKTIKG
                     YGMGASGEGQNVAHQAKKMDKASLKQFRDRFDIPVTDEQIESGDLPYLTFAPDTEEYK
                     YLHARRDALGGYLPQRKPTQEVLEVPELSAFDAQLKSSGEREFSTTMAFVRILSTLLK
                     DKKIGKRVVPIVPDESRTFGMEGMFRQYGIWNPKGQQYTPQDKDQLMFYKESVDGQIL
                     QEGINEPGAMADWIAAATSYANSNFAMIPFYIYYSMFGFQRIGDLAWAAGDMHARGFL
                     LGGTAGRTTLNGEGLQHEDGHSHIQADLIPNCVSYDPTFQYEVAVIVQDGLRRMYANN
                     EDVFYYITLMNENYTHPDMPEGAEQDILKGMYLLKAGGKGDKKVQLMGSGTILQEVIA
                     GAELLKADFGLEADIWSCPSFNLLHRDAVEVERFNRLHPLEAEKVPFVTSQLQGHDGP
                     VIAATDYIRSYADRIRAYIPNDYHVLGTDGFGRSDSRANLRRFFEVDRYNVAVAALAA
                     LAEQGKVSKETVQQAIEKYGIKADSAPSWKR"
     gene            7064..8776
                     /locus_tag="ACFM8S_000773"
     CDS             7064..8776
                     /locus_tag="ACFM8S_000773"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFC6313873.1"
                     /translation="MKASQFFISTLKEAPAEAALASHKLMIRAGLIKANASGLYTWMP
                     MGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHDR
                     DFCMGPTCEEVIADIVRKEINSYKQLPKNFYHIQTKFRDEVRPRFGVMRAREFVMKDA
                     YSFHADYASLQTTYDAMYDAYCRIFTRLGLEFRPVAADTGSIGGTGSHEFQVLAESGE
                     DVIAYSDTSDYAANIELAPTLPLKGERAAAQAELVKVHTPNVKTIESLVEFLNIPVEQ
                     TLKSIVVEGENEGEIVLLLLRGDHEFNDIKAEKLAGVKSPLTMASPAAIVEQFGANGG
                     SLGPVGFAGKVYADFATEKDGDWVIGANEDGYHYTGFNFGRDAAEPEFVDLRDVVEGD
                     ESPDGQGRLKLARGIEVGHVFQLRDKYTQAMNVSFLDNNGKSQIMEMGCYGIGITRVV
                     AAAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDTVREAADKIYAELLAAGADVLLD
                     DRDERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRDNEAQAIAIGEIVARVTA
                     SLNA"
     gene            8839..9495
                     /locus_tag="ACFM8S_000774"
     CDS             8839..9495
                     /locus_tag="ACFM8S_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFC6313874.1"
                     /translation="MASVFLEGEAVEVGNIFCIGRNYAAHIEELKNEIPSEPVVFMKP
                     SGSILNSGGTILLPEFSRDVQFECELVLLVGKDSDGTGEGKDILGCVAGYGVGLDLTA
                     RDIQCRLKEKGLPWLKAKGFRHSACVSDFAAAGRIGNPEKVLFSLKQNGVLKQRGDTG
                     LMIYPIREILHKLAADYGLGKGDLVFTGTPSGVGAIGAGDNLALELDGLVRASFTVGC
                     "
     gene            complement(9505..9960)
                     /gene="ruvX"
                     /locus_tag="ACFM8S_000775"
     CDS             complement(9505..9960)
                     /gene="ruvX"
                     /locus_tag="ACFM8S_000775"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFC6313875.1"
                     /translation="MHKIPKGTALAFDFGEARIGVAQGDAELGLSHPLSTVTGGSNDE
                     KFAAIAKLVQEWQPRYFVVGLPVHTDGTKHEMTHLSRKFGRRLNGRFNLPVYWVDERL
                     SSVYAESLLSEAQVFGKKRKSVLDQVAAQAILHGFFEGGPAECFNGREG"
     gene            complement(9953..10501)
                     /locus_tag="ACFM8S_000776"
     CDS             complement(9953..10501)
                     /locus_tag="ACFM8S_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFC6313876.1"
                     /translation="MNLSNHFLVAMPDMEDAFFSQSVVYICKHDEDGALGIAINKPSP
                     ITMDMIFSATGKNIPMRMQHDSVMMGGPVQVERGYVVHTPIGNWQSSIGVSDNIALTS
                     SRDVIENISREGAVDKALISIGYSSWGKGQLERELADNAWLTVPADEHILFDIPYEHR
                     YAAAFAKLGIDPLALFSGAGHA"
     gene            complement(10503..11063)
                     /locus_tag="ACFM8S_000777"
     CDS             complement(10503..11063)
                     /locus_tag="ACFM8S_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HFC6313877.1"
                     /translation="MNRLLLLSAAVLLTACGSGETDKIGRASTVFNILGKNDRIEVEG
                     FDDPDVQGVACYISYAKKGGLKEMVNLEEDASDASVSCVQTASSISFDETAVRKPKEV
                     FKHGASFAFKSRQIVRYYDPKRKTFAYLVYSDKIIQGSPKNSLSAVSCFGGGIPQTDG
                     VQADTSGNLLAGACMISNPIENPDKR"
     gene            11391..13181
                     /locus_tag="ACFM8S_000778"
     CDS             11391..13181
                     /locus_tag="ACFM8S_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HFC6313878.1"
                     /translation="MRYKPLLLALMLVFSTPAVAAHDAAHNRSAEVKKQTKNKKEQPE
                     AAEGKKEKGKNGAVKDKKTGGKEAAKEGKESKKTAKNRKEAEKEATSRQSARKGREGD
                     KKSKAEHKKAHGKPVSGSKEKNAKTQPENKQGKKEAKGQGNPRKGGKAEKDTVSANKK
                     VRSDKNGKAVKQDKKYREEKNAKTDSDELKAAVAAATNDVENKKALLKQSEGMLLHVS
                     NSLKQLQEERIRQERIRQARGNLASVNRKQREAWDKFQKLNTELNRLKTEVAATKAQI
                     SRFVSGNYKNSQPNAVALFLKNAEPGQKNRFLRYTRYVNASNREVVKDLEKQQKALAV
                     QEQKINNELARLKKIQANVQSLLKKQGVTDAAEQTESRRQNAKIAKDARKLLEQKGNE
                     QQLNKLLSNLEKKKAEHRIQDAEAKRKLAEARLAAAEKARKEAAQQKAEARRAEMSNL
                     TAEDRNIQAPSVMGIGSADGFSRMQGRLKKPVDGVPTGLFGQNRSGGDVWKGVFYSTA
                     PATVESIASGTVSYADELDGYGKVVVVDHGENYISIYAGLSEISVGKGYMVAAGSKIG
                     SSGSLPDGEEGLYLQIRYQGQVLNPSSWIR"
     gene            13301..14785
                     /locus_tag="ACFM8S_000779"
     CDS             13301..14785
                     /locus_tag="ACFM8S_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HFC6313879.1"
                     /translation="MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDRRDNEVLPVQ
                     SIRTMAEVYGQIKANYYQDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSG
                     EFGGLGMEIGQEDGFVKVVSPIEDTPAERAGVKSGDFIVKIDNVSTRGMTVSEAVKKM
                     RGKPGTKITLTLSRKNADKPIVVNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQERTVE
                     SVNTAAKELVKENKGKPLKGLVLDLRDDPGGLLTGAVGVSAAFLPSEAVVVSTKGRDG
                     KDRMVLKAVPEDYVYGMGGDSLAGIPAELKTIPMTVLVNSGSASASEIVAGALQDHKR
                     AVIVGTQSFGKGSVQTLIPLSNGSAVKLTTALYYTPNDRSIQAQGIVPDVEVKDKERI
                     FESREADLVGHIGNPLGGEDVNSETLAVPLEKDADKPAVKEKGKKKKDEDLSSRRIPN
                     PAKDDQLRKALDLVKSPEQWQKSLGLAAKKPVSNKDKKDKKDKK"
BASE COUNT         3539 a         3691 c         4194 g         3456 t
ORIGIN      
        1 ccggaggaaa tattttaatc cgctgtaaaa tagaaaatgc cgtctgaaca tttcagacgg
       61 catttgctca aataaacctt atcggctgtt tattcagtcg gatttatttt ttcttttgcg
      121 gaggcaggtc ggtacaagta cccaatgcca cttccgccgc cataccgatg gattcgccca
      181 aggtcgggtg cgggtggatg gttttgccga tgtctgccgc gtcgcagccc atttcgatgg
      241 caaggcagac ttcgccgatc atatcgccgc cgttaggacc aacgataccg ccaccgatga
      301 tgcggccggt ttcggcatca aaaatcagct tggtgaaacc attgtcgcaa ccgttggcaa
      361 tcgcacggcc ggaagcagcc cacgggaagc tggctttggt gattttgcgg ccggaggctt
      421 tggcggacaa ttcggtttcg cccacccacg ccacttcggg ggaagtgtag gcaacgcccg
      481 gaatcacgcg cgcgtcgaag taggctttgt ggccggcgca gttttcggcg gcaacgtggc
      541 cttcgtgaac ggctttgtgc gccaacatcg gctgaccgac gatgtcgccg atggcgtaga
      601 tgtgcggcac attggtacgc atttgtttgt ccacttcgat gaagccgcga tcggttacgg
      661 caacgcctgc tttttccgcg ctgatgagtt tgccgttggg cgcgcggccg gcggcaacca
      721 atacggcatc gtagcgttgc ggctctttag gcgcgttcgc gccttcaaag gtaacgtaaa
      781 cgccgtcttc tttcggctca actgcaacgg ttttggtgtt gaccataatg ttgtcaaaac
      841 ggtattcgtt ttgtttttgc catactttca ccaagtcgcg gtctgcgcct tgcatcaggc
      901 cgtccatcat ttcaacaaca tccaggcgag aacccagcgt gctgtaaacc gtacccattt
      961 cgagaccgat aataccgcca ccgatgatca gcagtttgcc cggcacttct ttcagcgcca
     1021 aagcgccgct ggagtcgaaa attcgaggat cttcagggat gaaaggcagt ttggttacgc
     1081 ggctgcctgc tgcaatgata cagtttttga aggcaacgat ttttttctcg ccggtagggg
     1141 ctgcctgttc gtacacatcg ccggcagtca gcgacacttc caagtggtgc ggatctaaga
     1201 attgcccgtc gccttggata acgtccactt tacggctttt cgccatacct gccaaaccgc
     1261 ccgtgaggcg ggaaactacg ccgtctttgt aggcgcgaag catatcgatg tcgagttccg
     1321 gctcggggta tttgataccg ttggcagcca agtggcgcac ttcgtcgata acggcggcat
     1381 tgtgcaacaa ggctttggaa gggatacagc cgacgttcag gcaaacgccg cccaaagttt
     1441 tgtaacgttc gacgatggcg actttcaagc cttcatcggc agcggcaaat gcagcggagt
     1501 aaccgccggg accgccaccc aatacgacca cgtcgtactc ggcatcggca gaaccgccga
     1561 attgcgcggc ttgcggagca ggagcagcag ctttaggggc ttcttgcgcc ggggcggcag
     1621 ccgcttcggc tttaggagcg gcagccgtgc cttcagcttc aacgacaaca atcaaaccgc
     1681 cttcggagat tttgtcgccg actttgactt tcacttcttt gaccacgccc gcaacttcgg
     1741 caggcacgtc catcgtcgct ttgtcggttt ccaaggtaat cagggtgtcg tcaacggcga
     1801 tggtgtcgcc cacgtttact tcaaccgcga taatatctac attttcgtgt ccgccaatgt
     1861 cgggcacttt caattcaact aagctcatgt ctcatctttc tgaatgatgt tcaaacttcc
     1921 ttttcagacg gcacagaccg ctatgccgtc tgaagccgat cgggttacag ggtaatgcgg
     1981 cggaagtctt tcaacagctt cgccaagaat acggtgaagc gcataccggc cgcaccgtcg
     2041 atgacgcggt ggtcgaagga caggctcaac gggcacatca ggcgcggggc aaactctttg
     2101 ccgttccaaa ccggtttgat ttgggatttg cacacgccca agatggcgac ttcgggagcg
     2161 ttcacaatcg gcgtgaaacc tgtgccgccg atgccgccca agctggaaat ggtaaagcac
     2221 gcgccttgca tttcttgcgg tttgagcttg ccttcgcggg cttttttaga caattcggtc
     2281 agctcttggc tgatttgttt caagcctttt tgatccacgt ctttgatgac gggaacaacc
     2341 aagccgttcg gcgtatcggc tgcgaaaccg atgttgaagt agtttttcag caccaggttg
     2401 tcgccgtcca aagaagcgtt gaattcgggg aaggctttca gcgcggaaac ggaggctttg
     2461 atgatgaacg ccagaggaga cagtttcacg ccttcgcgtt cccattcttt gttcagttgt
     2521 ttgcggaatt cttccagctc ggtcatatcc gcttcttcgt gtacggtaac gtgcggaatc
     2581 acaacccagt tgcgagacag gttttgaccg gagattttct taatgcggga caattcttta
     2641 acttcgacat tgccgaattt ggagaagtcc actttaggcc acggcagcaa gtccagaccg
     2701 ctgcccaaag atgcgccggc tgcggcaggt tttgccgcgc caccctgcat tacggatttc
     2761 acaaaggctt tgatgtcgtc gcccacgatg cggcctttca agccggtacc tttgacttgg
     2821 cccaaatcca cgcccaattc gcgcgccagt ttgcgtgcgg aagggccggc gtgtgctttg
     2881 gcgaaagcgg cctcgtcgat tttggcagcg gcaggcgcgg cggcgggtgc ggcggcagca
     2941 gtcggaggcg gagcggcagc cggtgcggca gcttgagcgg cttgagcagg ggctgctgcg
     3001 gcagagccga cggtttctac ttcgataatg gcagagcctt cggatacttt gtcgccgact
     3061 tttaagaata cggctttaac gacaccggca gcggtacaag gtacgtccat tgtcgcttta
     3121 tcggtttcca aagtaatcag cgtgtcgtct tcggcaacgg tgtcgcccac tttgatctcg
     3181 acggcgatta catccacatc ggtatggccg ccgatatcgg gaacggctac ttgaacggtt
     3241 gcaccgcctg cggcaacggg tgcggcagca ggtgcaggtt gtgcttcggc agcagcagga
     3301 gcggcttcgg cagcggcggc accggtttca acggtcaaaa ttacgccgcc ttcggagatt
     3361 ttgtcgccga ctttgacttt cacttctttc acgacaccgg ccgcatcggc aggcacatcc
     3421 atcgtggctt tgtcggtttc cagtgtaatc agggtgtcgt caacggcgat ggtgtcgccc
     3481 gctttaactt ctacggcgat gatgtcgacg ttttcgtgac cgccgatatc ggggactttg
     3541 atttctacga tactcatttg agttccttta atctttttca attggatgcc gtctgaaagc
     3601 tgcttcaggc ggcatcggta cggtttgtct gctcaaacca ttcgggcatt cggttttcag
     3661 acggcctgat gtcggaatgg ggcaaacagg ccgtctgaaa catcaatcag cgtttccagc
     3721 taggagctga atcggctttg atgccgtatt tctcaatggc ttgttgaacg gtttctttgc
     3781 tgactttgcc ttgttccgcc aatgcggcca atgcggccac ggcaacgttg tagcgatcca
     3841 cttcaaagaa gcggcgcagg ttggcgcgac tgtcggaacg gccgaaaccg tcagtgccca
     3901 agacgtggta gtcgttcggg atgtaggcgc ggatacggtc agcatagctg cggatatagt
     3961 cggtagcggc aataaccgga ccgtcatgac cttgcagttg ggaagtaacg aaaggtactt
     4021 tttcggcttc cagcggatgc aggcggttga agcgttctac ctcgacagcg tcgcggtgca
     4081 gcaggttgaa ggatgggcaa gaccaaatgt ctgcttccaa gccgaagtcg gctttcagca
     4141 gctcggcacc ggcaatcact tcttgcaaaa tcgtaccgga acccatcagt tggactttct
     4201 tgtcgccttt gccgccggct ttcagcaggt acataccttt caagatgtct tgttccgcac
     4261 cttcgggcat atccggatgg gtgtagttct cgttcatcag ggtgatgtag tagaacacgt
     4321 cttcattatt ggcatacata cggcgcagac cgtcttgtac gatgacggcg acttcgtatt
     4381 ggaaagtcgg gtcataagat acgcagttcg gaatcaggtc ggcctggatg tggctgtggc
     4441 cgtcttcgtg ttgcaggcct tcgccgttca gtgtcgtacg gccggcagta ccgcccagca
     4501 ggaagccgcg cgcgtgcata tcgcccgccg cccaagccaa gtcgccgata cgttggaaac
     4561 cgaacataga atagtaaatg tagaacggaa tcatggcgaa gttgctgttg gcgtagctgg
     4621 ttgcagccgc aatccagtcg gccatcgcgc ccggttcgtt aataccttct tgcaagattt
     4681 gaccgtcaac ggattcttta tagaacatca gttggtcttt gtcttgaggg gtatattgct
     4741 gacctttcgg attccaaata ccgtattggc ggaacatacc ttccatgccg aaagtacggc
     4801 tttcgtcggg aacgataggt acgacgcgtt tgccgatttt tttgtctttc agcaaagtgg
     4861 acaggatgcg gacgaatgcc atcgtggtcg agaactcgcg ttcaccgctg gatttgagtt
     4921 gtgcgtcgaa tgctgacagc tcgggcactt ccaatacttc ctgcgtcggt ttgcgttgcg
     4981 gcaggtagcc gcccaaagca tcgcggcgtg cgtgcaggta tttgtattct tccgtatcgg
     5041 gggcaaaagt caggtaaggc agatcgccgc tttcgatttg ttcgtcggta accggaatgt
     5101 caaagcggtc gcggaattgt ttcagggacg ctttgtccat ttttttggct tggtgggcaa
     5161 cgttctgacc ttcgccggat gcgcccatac cgtaaccttt aatggttttc gccaagatga
     5221 cggtaggttt gccgtcggca tggttcgctg cgcggtcgta ggcgttgtac actttttgcg
     5281 ggtcgtggcc gccgcggttc aatgcccaga gttgctcatc ggtcatatcg gcaaccaatg
     5341 ctttcagttc gggcgtattg aagaagtgtt cgcgcacata cgcgccgtct ttggatttgt
     5401 aagtttggta gtcgccgtcc aaacattctt ccatacgttg gcgcaggata ccgtctttgt
     5461 ctttcgccaa gaggcggtcc cagcggcggc cccaaatgac tttgacgaca ttccagccgg
     5521 cgccggcaaa gttgccttcc aattcttgga tgattttgcc gttgccgcgt accggaccgt
     5581 ccaagcgttg cagattgcag ttgatgacga aaatcaggtt gtccaagcct tcgcgtgcag
     5641 ccagtgcgat tgcaccttga gattcgggtt cgtccatttc gccgtcgccg cagaaacacc
     5701 atactttacg gcctttggtt tttgccaaac cacgcgattc caagtatttc aggaaacgcg
     5761 cctgataaat cgccatgatg ggtcccaagc ccatggatac ggtcgggaac tgccaaaagt
     5821 cgggcaagag gtgggggtgc ggataggaag gcagaccgtg tccgtccact tcttggcgga
     5881 agttgttcag ctggtcttcg gtcagacggc cctcgacgaa tgcgcgtgca tagatgcccg
     5941 gggcaacgtg accttggaag aagaccaaat cgccttcttc gccttcgccc ttggctttcc
     6001 aaaagtggtt gaaaccgact tcatacatgg tggcggcaga ttggaaagat gcgatgtgcc
     6061 cacccagttc caaatctttc ttgccggcgc gcagtacgat ggcggcggcg ttccagcgca
     6121 cgaatgcgcg gatgcggtgt tcgatgtttt ggtcgcccgg aatgcctttt tcgttttcga
     6181 cggaaacggt attcaaatac ggggtggtcg tgccgtgtgg catacgtacg cccttgtcgc
     6241 ggcagtattt gaccaggttt tccaagaggt attgcgcgcg ttcgccgcct tcatattcga
     6301 ggacggagct taacgcgtcc agccactctt gggtttcgat agggtcaaca tcgtgtaatt
     6361 gggtggacat agtatctatc ctttatgttg agtgtttaat cggcagggag ggtgtagcca
     6421 acccctttat tttcgtttgt gaaaaataat aagcaaaaac gaaaatttct aaatcaaacc
     6481 gaaaaaaata atttaagttt aatgttttca aaaaattata aaagttttgg aaacaaggga
     6541 aaacttgaaa aaaatgttcg atctgctcca cacgaaatcc tatcagcaat cgggtctgct
     6601 ggcaaataac catgcctgta aaagcaggtt ttatactgaa gttggcgttt ggtaaggtca
     6661 agtctttttg atttcgaaat attctgtttg attttggcga tattttttcg gaattgtggt
     6721 atatcgaatc attatgtctc tgtcggcaat tttcctattg tagaaaatct atacacaagc
     6781 tttgtggata accgtgttga taagccacgg cttgtgttaa aaaagtctta cgcaacaaat
     6841 gtacgcttat tttgattaaa aaataggcac atttaaatct ataaaaatca tgtgcttatg
     6901 ttcaatatga tgtttctggt catgtttttg ccgtctgtta tgggatttgt cccgcgcttg
     6961 gggcaggggg tgaattttgg aggttggtgc aaaacctgct tttttcgcgt tacaatctgc
     7021 atccgattta cttttgaatt tgcctgtttg gaaaaatact tttatgaaag ccagccaatt
     7081 ctttatctct actttaaaag aagcccctgc cgaagccgcg cttgccagcc acaagctgat
     7141 gattcgcgcc ggtctgatta aagccaacgc gtccggcctt tatacttgga tgccgatggg
     7201 gctgcgcgtg ttacgcaaag tcgaaaacgt cgtgcgcgag gaaatggcgc gtgcgggcag
     7261 cgtggagttg ctgatgccgg tggtgcagcc tgccgagctg tggcaggaat ccggccgctg
     7321 ggaattttac ggtaaagagt tgctgcgcct gaaagaccgc cacgaccgcg atttctgcat
     7381 gggtccgacc tgtgaagaag tcatcgccga catcgtgcgc aaagaaatca acagctacaa
     7441 acaactgccg aaaaactttt accacatcca aaccaaattc cgtgacgaag tgcgcccgcg
     7501 tttcggcgtg atgcgcgcgc gcgagttcgt catgaaagac gcttattcct tccatgccga
     7561 ctacgcctcg cttcagacga cctatgatgc catgtatgac gcttactgcc gtatcttcac
     7621 ccgtctgggc ttggaattcc gccccgttgc cgcagatacc ggcagcatcg gcggtacggg
     7681 ttcgcacgag tttcaagtgt tggcggaaag cggcgaagat gttattgcgt acagcgacac
     7741 ttccgattac gccgccaata tcgagttggc cccgaccttg ccgcttaaag gcgaacgcgc
     7801 cgctgctcag gcagaactcg tcaaagtaca cacgccaaac gtcaaaacca ttgagtcttt
     7861 ggttgaattc ctgaatattc cggttgaaca aaccctcaaa tccatcgtgg ttgaaggcga
     7921 aaacgaaggc gaaatcgttt tattgctgtt gcgcggcgac catgagttca acgacatcaa
     7981 agccgaaaaa ctcgcaggcg tgaaatcgcc attgacgatg gcaagtcctg ccgcgattgt
     8041 cgaacagttc ggcgcaaacg gcggctcgct gggtccggtc ggctttgcag gtaaagttta
     8101 tgccgatttc gccactgaaa aagacgggga ctgggtcatc ggtgcgaacg aagacggcta
     8161 ccactacacc ggcttcaact tcgggcgcga cgctgccgag cctgaatttg tcgatttgcg
     8221 cgatgtcgtc gaaggtgacg aaagccccga cgggcaaggc cgtctgaaac tggcgcgcgg
     8281 catcgaagtc ggacacgttt tccaattgcg cgacaaatac acccaagcca tgaacgtaag
     8341 cttccttgac aacaacggca aatcgcaaat catggaaatg ggctgctacg gtatcggcat
     8401 cacccgcgtc gtcgccgccg ccatcgagca gaataacgac gaaaaaggca tcatctggac
     8461 caaagcaatg gcgccgtttg aagtcgttat cgtgccgatg aactacaaaa aatcagacac
     8521 cgtacgcgaa gccgccgaca aaatctatgc cgaattgctg gcggcaggcg cggatgtgct
     8581 gctggatgac cgcgacgaac gcgcaggcgt attgctgaac gattccgagc tgttgggcat
     8641 cccgcaccgc atcgtcatcg gcgaccgcgc cttgaaagaa ggcaatgtcg aatacgccga
     8701 acgccgcgac aacgaagcgc aggcaattgc aatcggagaa attgttgcac gtgtaacagc
     8761 ttcattaaat gcgtaagctt ggtcgaatgc cgtctgaaaa taatttcaga cggcattttt
     8821 acttaggggg aaagtgaagt ggcttcagta tttttagaag gagaggcagt cgaggtcggc
     8881 aatattttct gtatcggcag gaactatgcc gcacatatcg aagagttaaa aaacgaaata
     8941 ccttccgaac ctgtggtgtt tatgaagccg tcaggcagca ttttgaacag cggcggtaca
     9001 atcttgctgc ccgagtttag tagggatgtg cagtttgagt gcgaactcgt gctgctggtc
     9061 ggtaaggatt cagacggcac gggcgagggt aaagatattt tgggatgtgt cgcaggatat
     9121 ggtgtagggc tggaccttac ggcacgggat atccaatgcc gtctgaagga aaaagggctg
     9181 ccctggctga aggcaaaagg gttcaggcat tccgcgtgcg tgtcggattt tgcggcggca
     9241 ggcagaatcg gcaatcccga gaaggtgttg ttttcattaa aacaaaacgg cgtattgaaa
     9301 cagcgcgggg ataccggttt gatgatttac ccgattcggg aaattttaca caaactggcg
     9361 gcggattacg gtttggggaa aggggatttg gtgtttaccg gaacgccctc cggcgtgggc
     9421 gcaatcggtg cgggggacaa tttggcattg gagctggacg gcttggtgcg cgcctctttc
     9481 acggtagggt gttaaccgcg ccgcttaacc ctcacgcccg ttgaaacatt ccgcaggacc
     9541 gccctcgaaa aaaccgtgca agatggcttg cgccgccact tggtcgagca ccgatttgcg
     9601 ttttttgccg aagacctgtg cttccgaaag caggctttcg gcatagacgg acgacagccg
     9661 ttcgtcaacc caatagacgg ggagattgaa cctgccgttc agcctgcgtc cgaacttgcg
     9721 cgacaggtgc gtcatttcat gtttcgtgcc gtcggtatgc acgggcagtc cgacgacaaa
     9781 ataacgcggc tgccattctt gaaccagctt ggcgattgcc gcgaactttt catcgttgct
     9841 gccgccggta acggtgctca aaggatggga tagccctaat tccgcgtctc cttgtgccac
     9901 gccgatacgc gcttcgccga agtcgaatgc cagtgccgtt ccttttggaa ttttatgcat
     9961 ggccggctcc cgaaaacagg gcgagcgggt cgatgccgag tttggcgaat gcggcggcgt
    10021 aacggtgttc gtaggggatg tcgaacagga tgtgttcgtc ggcgggaaca gtcagccacg
    10081 cattgtcggc aagttcgcgt tcgagctggc cttttcccca gcttgaatag cctatgctga
    10141 tcaaggcttt gtcaaccgca ccttcgcgtg aaatattttc aatcacgtct cgggaagaag
    10201 ttagcgcgat attgtctgaa acgccgatac tgctttgcca gttgccgatc ggggtatgca
    10261 cgacataacc tcgctcgact tgcaccggcc cgcccatcat cacgctgtcg tgctgcatcc
    10321 gcatggggat gtttttgccg gtggcggaaa aaatcatgtc catcgtaatc ggagagggtt
    10381 tgttgatggc gatgccgagt gcgccgtctt catcgtgttt gcagatatag acgaccgatt
    10441 gtgaaaaaaa cgcgtcttcc atatcgggca tggcgacgag aaagtggttg gagaggttca
    10501 tatcagcgtt tgtcgggatt ttctatcggg ttggaaatca tgcaggcgcc ggcaagcagg
    10561 ttgccggaag tatcggcttg caccccatcg gtttgcggta tgccgccgcc gaaacaggaa
    10621 accgcgctta aggaattttt cggcgagcct tggatgattt tatcgctgta caccaaatag
    10681 gcgaaggttt tgcgtttggg gtcgtaataa cggacaatct gccggctctt gaacgcgaag
    10741 ctcgcaccgt gtttgaaaac ttctttcggt ttgcgcacgg cggtttcgtc aaaagaaatc
    10801 gaagatgccg tctgaacgca cgaaaccgat gcgtcggacg cgtcctcttc caaattgacc
    10861 atttccttca agccgccttt ttttgcatac gaaatataac aggcaacccc ttgaacgtcg
    10921 ggatcgtcga atccttccac ttcgatacgg tcgtttttgc ccagtatgtt gaaaacggta
    10981 cttgcccgtc cgattttatc ggtttcgccg ctgccgcagg cagtcagcag gacggcggca
    11041 gacagcagta gcagtctgtt catcaaaatt tccttatcgg ttgaatcccc gttttccgtg
    11101 aaggcggcag ggcttgcttt gtttggaggg gcgcacgttc ccaaaatcgg gcaacatacg
    11161 gtatgcggct taatgtgcta ccctgtgtgt cggaacacaa tatccgttgc ggcattgaaa
    11221 atatgacgca caatataagg ctgtttgagc cgattgcaag tatccgcgca aagaaggcgc
    11281 aagattgggc aaacggcatt ttcagacggc ataactgaca gtataatcca aacgtccggc
    11341 tgctttcgtg cagccttcag tattatccaa ctttttccga aggtcgaacc atgcgctaca
    11401 aaccccttct gcttgccctg atgctcgttt tttccacgcc cgccgttgcc gcccacgacg
    11461 cggcacacaa ccgttccgcc gaagtgaaaa aacagacgaa gaacaaaaaa gaacagcccg
    11521 aagcggcgga aggcaaaaaa gaaaaaggca aaaatggcgc agtgaaagat aaaaaaacag
    11581 gcggcaaaga ggcggcaaaa gagggcaaag agtccaaaaa aaccgccaaa aaccgcaaag
    11641 aagcagagaa ggaggcgaca tctaggcagt ctgcgcgcaa aggacgcgaa ggggataaga
    11701 aatcgaaggc ggaacacaaa aaggcacatg gcaagcccgt gtccggatcc aaagaaaaaa
    11761 acgcaaaaac acagcctgaa aacaagcaag gcaaaaaaga ggcaaaagga cagggcaatc
    11821 cgcgcaaggg cggcaaggcg gaaaaagaca ctgtttctgc aaataaaaaa gtccgttccg
    11881 acaagaacgg caaagcagtg aaacaggaca aaaaatacag ggaagagaaa aatgccaaaa
    11941 ccgattccga cgaattgaaa gccgccgttg ccgctgccac caatgatgtc gaaaacaaaa
    12001 aagccctgct caaacaaagc gaaggaatgc tgcttcatgt cagcaattcc ctcaaacagc
    12061 ttcaggaaga gcgtatccgc caagagcgta tccgtcaggc gcgcggcaac cttgcttccg
    12121 tcaaccgcaa acagcgcgag gcttgggaca agttccaaaa actcaatacc gagctgaacc
    12181 gtttgaaaac ggaagtcgcc gctacgaaag cgcagatttc ccgtttcgta tcggggaact
    12241 ataaaaacag ccagccgaat gcggttgccc tgttcctgaa aaacgccgaa ccgggtcaga
    12301 aaaaccgctt tttgcgttat acgcgttatg taaacgcctc caatcgggaa gttgtcaagg
    12361 atttggaaaa acagcagaag gctttggcgg tacaagagca gaaaatcaac aatgagcttg
    12421 cccgtttgaa gaaaattcag gcaaacgtgc aatccctgct gaaaaaacag ggtgtaaccg
    12481 atgcggcgga acagacggaa agccgcagac agaatgccaa aatcgccaaa gatgcccgaa
    12541 aactgctgga acagaaaggg aacgagcagc agctgaacaa gctcttgagc aatttggaga
    12601 agaaaaaggc cgaacaccgc attcaggatg cggaagcaaa aagaaaattg gctgaagcca
    12661 gactggcggc agccgaaaaa gccagaaaag aagcggcgca gcagaaggct gaagcacgac
    12721 gtgcggaaat gtccaacctg accgccgaag acaggaacat ccaagcgcct tcggttatgg
    12781 gtatcggcag tgccgacggt ttcagccgca tgcaaggacg tttgaaaaaa ccggttgacg
    12841 gtgtgccgac cggacttttc gggcagaacc ggagcggcgg cgatgtttgg aaaggcgtgt
    12901 tctattccac tgcaccggca acggttgaaa gcattgcgtc gggaacggta agctatgcgg
    12961 acgagttgga cggctacggc aaagtggtcg tggtcgatca cggcgagaac tacatcagca
    13021 tctatgccgg tttgagcgaa atttccgtcg gcaagggtta tatggtcgcg gcaggaagca
    13081 aaatcggctc gagcgggtcg ctgccggacg gggaagaggg gctttacctg caaatacgtt
    13141 atcaaggtca ggtattgaac ccttcgagct ggatacgttg atcggattgc cgaaacggta
    13201 ggctttatgc aaatcgtgcc gatatggtgc gctgttccgt gaagtttgcc gtacctgtat
    13261 tttccggttt ttggaaatca agagagaaag ttaaagaaga atgtcgaaac ctgtttttaa
    13321 gaaaatcgca ctttatactt tgggtgcaat cagcggcgtg gccgtcagtc tggcggtgca
    13381 gggttttgcc gccgagaagg acaggcggga taacgaagtc ctgccggtgc aatccatccg
    13441 cacaatggcg gaggtttacg gtcaaatcaa ggcaaactac tatcaggaca aacccgatgc
    13501 cgatttgttt gaaggtgcga tgaagggtat ggtggccggt ttggatccgc attccgaata
    13561 tatggataaa aaaggttatg ccgagataaa ggagtccacc agcggcgaat ttggcggctt
    13621 ggggatggaa atcgggcagg aagacggatt tgtcaaagtg gtttcgccga ttgaggacac
    13681 gcctgcggaa cgggcggggg tgaaaagcgg cgatttcatt gtgaaaatcg ataatgtttc
    13741 gacacgcggc atgacggtca gcgaagcggt gaagaaaatg cggggcaagc cgggtacgaa
    13801 gattactttg acgctgtcgc gcaaaaatgc cgacaagccg atagtcgtca acctgacccg
    13861 tgccattatt aaagtgaaaa gcgtccgcca tcacctgatc gaacccgatt acggctatat
    13921 ccgcgtgtcg cagttccaag agcggacggt cgaaagcgtc aataccgccg caaaagagct
    13981 ggtaaaggaa aataaaggaa aaccgctcaa ggggctggtg ttggatttgc gcgacgaccc
    14041 cggcgggctt ttgactggcg cggtcggcgt gtcggcggca tttctgccgt ctgaagcagt
    14101 cgtcgtcagc accaagggac gcgacggcaa agaccgcatg gtactgaaag ccgttcctga
    14161 agattatgtg tacgggatgg gcggcgattc gttggcgggc attcctgccg agttgaaaac
    14221 cataccgatg acggtattgg tcaattccgg ttcggcttcc gcgtcggaga ttgtcgcagg
    14281 tgcattgcag gatcataaac gcgcggtcat cgtcggtacg cagagcttcg gcaaaggttc
    14341 ggttcagact ttgattcctt tgtccaacgg cagcgcggtc aagctgacaa cggcactgta
    14401 ttatacgccg aacgaccgtt ctattcaggc gcaggggatt gttcccgatg tcgaagtaaa
    14461 agataaggaa cgcatttttg aaagccgcga ggcggatttg gtcggacaca tcggcaatcc
    14521 tttgggcggc gaggatgtga acagtgaaac ccttgccgtg ccgcttgaaa aagatgcgga
    14581 taagcccgct gtaaaagaaa aaggtaaaaa gaaaaaggac gaggatttgt cttcaaggcg
    14641 gattcccaac cctgccaaag acgaccagtt gcggaaagct ttggatttag tcaagtcgcc
    14701 cgagcagtgg cagaagtctt tggggctggc ggcgaaaaag ccggtttcaa ataaagataa
    14761 gaaagataaa aaagataaga agtaggtttt gacgatgccg tctgaaagga tttcagacgg
    14821 catattatgt ttgaaaggac aggttatgag cgatttttta ccggaatttg aaccgtgcgg
//
LOCUS       DAXHSF010000033        14727 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.033, whole genome shotgun sequence.
ACCESSION   DAXHSF010000033 DAXHSF010000000
VERSION     DAXHSF010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14727
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.033"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            136..2016
                     /locus_tag="ACFM8S_000780"
     CDS             136..2016
                     /locus_tag="ACFM8S_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HFC6313880.1"
                     /translation="MNIFYEESGQFKIASIVQKNDATYQVDTPHGKRTKVKANNVFAE
                     FDGDMAAFLENAQAQAADIDTDLLWEVCGEEEFTAEAIAEEYYGHAPTKTELAATLIA
                     LYAAPVYFYKKAKGVFKAAPEETLKQALAAIERKKQQDAQIDAWAEALKRGEMPSEIA
                     ADLKTILHAPDKQSLTYKAFTKAADALKTSAYELAKKTGGITSIPQYLQDGFEIKYFP
                     KGTGFPDLALPEMPDLPKADVTAFSIDDESTTEVDDALSLTDLDNGTKRVGIHIAAPS
                     LAVKPGDKMEKNIMERLSTVYFPGGKITMLPENWIAAFSLDAGAYRPSISIYFDVDGE
                     FNVGEPTCKIEAVNIAENLRIQAIEPHFNAETGLDEAGEMMFAHHQDLIWFYQFATAL
                     QKARGKYEPDRAPQYDYSIELDEEGKVSVVRRERGSPIDTLVSEMMILANSTWAQMLH
                     DNDLPGLFRVQPAGKVRMSTKSEPHIGMGVQHYGWFTSPLRRAADYINQKQLISLIDD
                     TAEPLYQNSDAELFAALRDFDAAYTAYADFQRQMEAYWSLIYLQQQGTSELTATILKE
                     DLVRIEGLPLVTRANGIPFDALPKSQALFKITELDAEKQFVSLNYIKAAAPAGTTAGN
                     AV"
     gene            2073..2462
                     /locus_tag="ACFM8S_000781"
     CDS             2073..2462
                     /locus_tag="ACFM8S_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313881.1"
                     /translation="MLYKCKIKKLDNTIEEAVLIEINGYELHCFISYCPFKIFENQFY
                     EVELSYEIFNDYILDKSTSNIGFRKIDDSFKYEITAELKNNHLTLQNIQFEEDEYLIN
                     YPYLDNCLVTLTVDRISVSFIREIQAA"
     gene            2471..2854
                     /locus_tag="ACFM8S_000782"
                     /pseudo
     CDS             2471..2854
                     /locus_tag="ACFM8S_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233580.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
     gene            complement(3178..4284)
                     /locus_tag="ACFM8S_000783"
     CDS             complement(3178..4284)
                     /locus_tag="ACFM8S_000783"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC6313882.1"
                     /translation="MKIKALMIAAALLAAADVHAAPQKAKTASAKAAKAAKAAKAAKA
                     AKAAKAAKVAKVAKVAKVAKVAKVAKVAATAQKEAAPAQQQGGIRFSDGIAAVADNEV
                     ITRRRLAEAVAEAKANLPKEAQISESELSRQVLMQLVNQSLIVQAGKRRNIQASEAEI
                     DAVVAKNSALKNLSPAQRRDFADNIIAEKVRQQAVMQNSRVSEAEIDAFLEQAQKQGI
                     TLPEGAPLRQYRAQHILIKADSENAAVGAESTIRKIYGEARSGTDFSSLARQYSQDAS
                     AGNGGDLGWFADGVMVPAFEEAVHALKPGQVGAPVRTQFGWHIIKLNEVRDAGTPQER
                     IRNSVRQYIFQQKAEQATVNLLRDLHSGAYVDIR"
     gene            complement(4389..6797)
                     /locus_tag="ACFM8S_000784"
     CDS             complement(4389..6797)
                     /locus_tag="ACFM8S_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HFC6313883.1"
                     /translation="MARLFSLKPLVLALGFCFGTHCAAADAVAAEETDNPTAGGSVRS
                     VSEPIQPTSLSLGSTCLFCSNESGSPERTEAAVRGSGEASIPEDYTRIVADKVEGQSQ
                     VQVRAEGNVVVERNRTTLNTDWADYDQSGDTVTAGDRFALQQDGTLIRGETLTYNLEQ
                     QTGEAHNVRMETEHGGRRLQSVSRTAEMLGEGHYKLTETQFNTCSAGDAGWYVKAASV
                     EADREKGIGVAKHAAFVFGGVPIFYTPWADFPLDGNRKSGLLVPSLSAGSDGVSLSVP
                     YYFNLAPNLDATFAPSVIGERGAVFDGQVRYLRPDYAGQSDLTWLPHDKKSGRNNRYQ
                     AKWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGNKEIAGNVNLNRRVWLDYGGRAAGG
                     SLNAGLSVLKYQTLANQSGYKDKPYALMPRLSADWRKNTGRAQIGVSAQFTRFSHDSR
                     QDGSRLVVYPDIKWDFSNSWGYVRPKLGLHATYYSLNRFGSQEARRVSRTLPIVNIDS
                     GATFERNTRMFGGGVLQTLEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENLYY
                     GNDRINTANSLSAAVQSRILDGATGEERFRAGIGQKFYFKDDAVMLDGSVGKKPRNRS
                     DWVAFASGSIGSRFILDSSIHYNQNDKRAENYAVGASYRPAQGKVLNARYKYGRNEKI
                     YLKSDGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEVLAGAEYKSSCGC
                     WGAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPADRMDVAVPGYIPAHSLSAGR
                     NKRP"
     gene            6867..7871
                     /gene="amgK"
                     /locus_tag="ACFM8S_000785"
     CDS             6867..7871
                     /gene="amgK"
                     /locus_tag="ACFM8S_000785"
                     /EC_number="2.7.1.221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramate/N-acetylglucosamine kinase
                     AmgK"
                     /protein_id="HFC6313884.1"
                     /translation="MQRQTELKNWLQTIYPERDFDLSFAAADADFRRYFRATFSDGSS
                     VVCMDAPPDKMSVAPYLKVQKLFDMVNVPQVLHADTDLGFVVLNDLGNTTFLTAMLQE
                     QGETAHKALLLEAIGELVELQKASREGVLPEYDRETMLREINLFPEWFVAKELGRELT
                     FKQRQLWQQTVDTLLPPLLAQPKVYVHRDFIVRNLMLTRGRPGVLDFQDALYGPISYD
                     LVSLLRDAFIEWEEEFVLDLVIRYWEKARAAGLPVPEAFDEFYRWFEWMGVQRHLKVA
                     GIFARLYYRDGKDKYRPEIPRFLNYLRRVSRRYAELALLYALLVELVGDEELETGFTF
                     "
     gene            complement(7928..8626)
                     /locus_tag="ACFM8S_000786"
     CDS             complement(7928..8626)
                     /locus_tag="ACFM8S_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFC6313885.1"
                     /translation="MKSRQLALGVAALFALAACDSKVQTSVPADSAPAASAAAAPAGL
                     VEGQNYTVLANPIPQQQAGKVEVLEFFGYFCPHCAHLEPVLSKHAKSFKDDMYLRTEH
                     VVWQKEMLPLARLAAAVDMAAADSKDVANSHIFDAMVNQKIKLQEPEVLKKWLGEQTA
                     FDGKKVLAAYESPESQARADKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMNTID
                     LLADKVREEQKAAQ"
     gene            8653..8844
                     /locus_tag="ACFM8S_000787"
     CDS             8653..8844
                     /locus_tag="ACFM8S_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313886.1"
                     /translation="MLEINRNPAILLHFRVLIQFAAGKGRFRFGKPLQTASNPMPSEA
                     VSVVQYAPLPQTGTTVEEQ"
     gene            8844..10382
                     /gene="murJ"
                     /locus_tag="ACFM8S_000788"
     CDS             8844..10382
                     /gene="murJ"
                     /locus_tag="ACFM8S_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFC6313887.1"
                     /translation="MNMLGALAKVGSLTMVSRVLGFVRDTVIARAFGAGMATDAFFVA
                     FKLPNLLRRVFAEGAFAQAFVPILAEYKETRSKEATEAFIRHVAGMLSFVLVIVTALG
                     ILAAPWVIYVSAPGFAKDADKFQLSIDLLRITFPYILLISLSSFVGSVLNSYHKFSIP
                     AFTPTFLNVSFIVFALFFVPYFDPPVTALAWAVFVGGILQLGFQLPWLAKLGFLKLPK
                     LNFKDAAVNRVMKQMAPAILGVSVAQISLVINTIFASYLQSGSVSWMYYADRMMELPG
                     GVLGAALGTILLPTLSKHSANQDTEQFSALLDWGLRLCMLLTLPAAVGLAVLSFPLVA
                     TLFMYREFTLFDAQMTQHALIAYSFGLIGLIMIKVLAPGFYARQNIKTPVKIAIFTLI
                     CTQLMNLAFIGPLKHVGLSLAIGLGACINAGLLFYLLRRHGIYQPGKGWAAFLAKMLL
                     SLAVMGGGLWAAQACLPFEWVRAGGMRKAGQLCILIAVGGGLYFASLAALGFRPRHFK
                     RVEN"
     gene            10386..10517
                     /locus_tag="ACFM8S_000789"
     CDS             10386..10517
                     /locus_tag="ACFM8S_000789"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313888.1"
                     /translation="MPISFFGKRFSTPIFGRASPTSAPAGRAAASSMPSENKKTDTL"
     gene            10514..11470
                     /locus_tag="ACFM8S_000790"
     CDS             10514..11470
                     /locus_tag="ACFM8S_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheT family hydrolase"
                     /protein_id="HFC6313889.1"
                     /translation="MILTPPDTPFFLRNGNADTIAAKFLQRPAPTYRRELLPDSTGKT
                     KVAYDFSDGISPDAPLVVLFHGLEGSSRSHYAVELMLAVRSRGWHGAVVHFRSCGGIA
                     NTAPVFYHLGDTAEIAFTLDTLAARYREIYAVGVSLGGNALAKYLGEQGENALPQAAA
                     VISAPVDAEAAGRRFDSGITRLLYTRYFLRTLIPKARTVPGFQTAFAAGCKTLGEFDD
                     RFTAPLHGFADRHDYYRRTSCKPLLKHVAKPLLLLNAANDPFLPPEALPRTDEVSEAV
                     TLFQPAHGGHVGFVGSTGGRLHLQWLPQTVLSYFDSFRTNSR"
     gene            11520..12302
                     /gene="trpC"
                     /locus_tag="ACFM8S_000791"
     CDS             11520..12302
                     /gene="trpC"
                     /locus_tag="ACFM8S_000791"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HFC6313890.1"
                     /translation="MTDILNKILATKAQEVAAQKAAVNAEHIRALAENAPPVRSFIDS
                     IRGKHRLNLPAVIAEIKKASPSKGLIRPDFRPAEIARAYENAGAACLSVLTDEPYFQG
                     SPEYLKQAREAVSLPVLRKDFIIDEYQVYQARAWGADAVLLIAAALEQEQLERFEAVA
                     HELGMTVLLELHDETELEKCRNLTTPLWGVNNRNLRTFEVSLDQTLSLLPALEGKTVV
                     TESGITGKADVEFMRARGVHTFLIGETFMRADDIETEVGKLF"
     gene            12367..14667
                     /gene="recQ"
                     /locus_tag="ACFM8S_000792"
     CDS             12367..14667
                     /gene="recQ"
                     /locus_tag="ACFM8S_000792"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFC6313891.1"
                     /translation="MHRPTAKQILHEVFGYPEFRGRQEAVINTLAGGRSLMVLMPTGG
                     GKSLCYQIPALMREGVAVVVSPLIALMNDQVASLHVAGIEAAAVNSGTSADEAREIAD
                     KLAQGRLKLLYVAPERLVTDRFLRFLDQQTVSLFAIDEAHCVSQWGHDFRPEYQQLGI
                     LAERYPNVPRIALTATADAATRADIKHYLHLDNAPEFVSSFDRPNIYYQVIEKNNGKK
                     QLLDFIRKEMTGQSGIVYCLSRKKVEDVAQFLRENGLNAIPYHAGLSMDVREENQRRF
                     THEDNIIVVATVAFGMGIDKPDVRFVAHLDMPQSVEHFYQESGRAGRDGLPAASWLCY
                     GLNDWVLLRERIAEGNSDEVQKQIEMQKLDAMLSVCETAACRRVLLLKHFGEESEPCG
                     HCDNCLHPPVRFDGTVLVQKLLSCVYRAGQRFAAGYITNLLRGKSDDWIRGNRHEQLS
                     TFGIGTELSDKEWRSVIRQCISLGYLTVNIARYQALQLTEAAKKVLKGETEVMLRPLK
                     RDKPAARTLKDNWLRTEREERLWQALRVWRMKQAEAEVIPAYMIFGDKTLRDLVEKMP
                     QDLNGLHDIYGLGEAKIDRFGHGILEVCRNTADFSHNAVIRPQTEREQQLRQKLEAWR
                     YEQARAENCALHAVLSDESLADMLAATPETETDLEGVHGLGSVRAAKYGRDILAVCRP
                     FSDGIDETAKHKRRLMRALIQWCNETAKHEQSEPYRILSKAALRTIAAKQPEGLAELA
                     AVYGVGEEKAARYGAAVLAVLERNAV"
BASE COUNT         3248 a         4071 c         4046 g         3362 t
ORIGIN      
        1 gtactggttt ttgttaatcc actataacgc aagcaccgca aagacgcgcc aaccctctcc
       61 caaccttttt tcagacggca ttttcggtaa tctgctaaaa tcgcccgctt gagtttccac
      121 agaaaaatcc gaaaaatgaa tattttttac gaagagtccg gccaattcaa aatcgcctct
      181 attgtccaaa aaaacgatgc cacctaccaa gtcgataccc cacacggcaa acgcaccaaa
      241 gtgaaggcga acaacgtctt tgccgagttt gacggcgata tggcggcgtt tttggaaaac
      301 gcgcaggcgc aggcggcgga catcgacacc gatttattgt gggaagtgtg cggcgaagag
      361 gaatttaccg ctgaagccat agccgaagaa tattacggcc atgcgccgac caaaaccgaa
      421 ctggcggcga ccttgattgc gctgtacgcc gcgccggtgt atttctacaa aaaagccaaa
      481 ggcgtgttca aagccgcgcc cgaagaaact ttaaaacaag cacttgccgc catcgaacgc
      541 aaaaaacagc aagacgcgca aatcgatgct tgggcggaag ccttgaaacg cggcgagatg
      601 ccgtctgaaa tcgcggcgga tttgaaaacc atcctgcacg cgcccgacaa gcagtcgctg
      661 acctacaaag cctttaccaa agccgccgac gcgctgaaaa cctctgccta cgaattggcg
      721 aaaaaaacgg gcggcattac gtccattccc caatacctgc aagacgggtt tgaaatcaaa
      781 tacttcccta aaggaacagg cttccccgac cttgcccttc ccgaaatgcc cgacctgccc
      841 aaggccgacg ttaccgcctt ttccattgac gacgaatcga ccaccgaagt ggacgacgct
      901 ttaagcctga ccgacttgga caacggcacg aagcgcgtcg gcatccacat cgccgcgccg
      961 tcgcttgccg ttaaaccggg cgacaaaatg gaaaaaaaca tcatggaacg cttaagcacg
     1021 gtttattttc ccggcggcaa aatcactatg ttgcccgaaa actggattgc cgcgttcagc
     1081 cttgatgcag gtgcataccg cccttccatc agcatttatt tcgatgtgga cggcgaattc
     1141 aacgtcggcg aaccgacctg caaaatcgaa gcggtcaaca tcgccgaaaa cctgcgtatc
     1201 caagccatcg agccgcattt caacgccgaa accggcttgg acgaagccgg cgaaatgatg
     1261 ttcgcccacc atcaagacct gatttggttc tatcaattcg ccaccgccct gcaaaaagcg
     1321 cgcggcaaat acgaacccga ccgcgcgccg caatacgatt acagcatcga attggatgag
     1381 gaaggcaagg tatccgtcgt ccgccgcgaa cgcggttcgc ccatcgatac gctggtcagc
     1441 gagatgatga ttcttgccaa cagcacttgg gcgcaaatgc tccatgacaa cgacctgccc
     1501 ggccttttcc gcgtccaacc ggcaggcaaa gtgcgcatga gcactaaatc cgagccgcac
     1561 atcggcatgg gcgtgcagca ttacggctgg ttcacttcgc cgctgcgccg cgccgccgac
     1621 tacatcaacc aaaagcagct gatcagcctg atagacgaca ctgccgagcc gctgtatcaa
     1681 aacagcgatg ccgagctttt cgccgcgctg cgcgactttg atgccgccta taccgcctac
     1741 gccgacttcc aacgccaaat ggaagcctac tggagcctca tctatctgca acagcaaggc
     1801 acgagcgagc tgaccgcgac catcctcaaa gaagacctcg tccgcatcga aggcctgccg
     1861 ctggtaacac gtgccaacgg catcccgttt gacgcgctgc ccaaatcaca ggcattgttt
     1921 aaaatcactg aattagatgc cgagaagcag tttgtctcgc tcaactacat caaggcagcc
     1981 gcacccgcgg gtacaacggc aggcaatgcc gtctgaagcc cgatacggca aactccggca
     2041 acaaaaaacg gacggcaaaa aggcaagata aaatgttata taaatgtaaa atcaaaaagt
     2101 tagataacac cattgaagag gcagtattga ttgaaataaa tggttatgaa ctacattgtt
     2161 ttatatcata ctgcccattc aaaatatttg aaaatcaatt ttatgaagtg gaattatcct
     2221 atgaaatctt taatgattac atactggata aatcaacttc aaatattgga tttagaaaaa
     2281 tagatgatag tttcaagtat gaaataacgg cagaattaaa aaataatcat ctaaccctac
     2341 aaaatataca atttgaagaa gatgagtatt taataaacta cccttatctt gataattgtt
     2401 tggttacatt aacggttgat agaataagtg tctcatttat tagagaaatt caggcagcct
     2461 gaaacctgtt gtggataccg ttggcagttt agcgattggt tatgccacag gcggcaatgc
     2521 tggggctttt actggtgcga atgcagattg gaataatcgg cagttgcatt cgagtgaata
     2581 taaattagca gaacaatatg cagacatagt agcttctcgt ctaaacattt caaaagaaga
     2641 agcacttggg cgaattattc ggcatatgca acgaaccgta gattacgcaa cggctaaagc
     2701 ggataatttc cgtactgatg acgccattat ttcaatttta ggagcaaaca atttacccaa
     2761 gcaagatcaa cattacacta atccgaacta caacaaacaa tacattcaaa attatgttcg
     2821 tgatttcaat ttagccaata aatacaatta ttttatgccg tccgttttta cttttatccc
     2881 gatttgcggg gcaagttttc caactgcatc ctgtcaaaaa attccattat tttcaaatac
     2941 ttaaaagtta aaaaggttaa atttttctta acaatcaaaa acattgttta acggaatatt
     3001 aacttaaaga gcagctagaa aacgagcttt tcgccgtcca ccggaaaact tttacggcgt
     3061 ttgtttattt ttttcaatat gttccaccgc ttcgtccgcc tgtctgccgc cgcagtttga
     3121 aaaagccgtg ccgcccgaag gttgtcggga cggcacggct tttcagtcac gaccgtttca
     3181 gcggatgtcc acgtaggcac cggaatgcag gtcacgcaac aggttgacgg ttgcctgttc
     3241 ggctttttgt tggaagatgt attgccgcac ggaattgcgg atacgttcct gaggtgtgcc
     3301 ggcatcgcgc acttcgttca atttgatgat atgccagccg aattgggtgc ggacgggcgc
     3361 gccgacctgt ccgggtttga gcgcgtggac ggcttcttca aaggcgggaa ccatcacgcc
     3421 gtcggcaaac cagcccaaat ctccgccgtt gcccgcgctc gcgtcttgcg aatattggcg
     3481 cgctaggctg gaaaagtctg tgccgctgcg ggcctctccg tagattttgc ggatggtgct
     3541 ttccgcgccg acggcggcgt tttcgctgtc ggctttaatc aggatgtgtt gggcgcggta
     3601 ttggcgcaac ggtgcgcctt cgggcagggt gatgccttgt ttttgcgcct gctcgaggaa
     3661 ggcatcgatt tcagcttcgc tcacgcggct gttctgcatc actgcctgct ggcggacttt
     3721 ttcggcaatg atgttgtcgg caaaatcgcg gcgttgggcg gggctgaggt ttttgagggc
     3781 ggaatttttt gcgacgacgg catcgatttc cgcttcgctt gcttgaatgt tgcggcgttt
     3841 gcccgcctgt acaatcaggg attggttgac aagctgcatc agcacctgtc gggacagctc
     3901 ggattcgctt atctgcgcct ctttgggcag gttggctttg gcttcggcaa cggcttcggc
     3961 aagccggcgg cgcgtgatga cttcgttgtc ggcaacggcg gcaatgccgt ctgaaaagcg
     4021 gataccgccc tgctgttgtg cgggtgcggc ttctttttgc gccgtggcgg caactttggc
     4081 aactttggca actttggcaa ctttggcaac tttggcaact ttggcaactt tggcagcttt
     4141 ggcagctttg gcagctttgg cagctttggc agctttggca gctttggcag ctttggcgga
     4201 tgcggttttt gccttttgcg gtgcggcgtg gacatcggct gctgccagca atgcggcggc
     4261 aatcatcagg gctttgattt tcatcattct ttccttatcg tttgaatcct acctttgatg
     4321 gcgggtttgg ggttgtcggt ggaatgccgt cttgttcggg aacggtgctc ctgccgggtt
     4381 tccgacggtc agggtcgttt gttgcgtccg gcggaaagag agtgagcggg gatatagccg
     4441 ggaacggcga catccatcct gtctgcgggg tttctgccga cactgctgag gtctttcaac
     4501 tgaagtgaga aaaagacagc gtttttgtag gtgttttcgc cggtaacgta gcgttgggcg
     4561 tacacgcccg cgccccagca gccgcaactg cttttgtatt ccgcacccgc cagcacctct
     4621 atcggttttt tggcttcaaa accgtagttg taacggacga cggccgacag gttgcgcgtc
     4681 agcggccatt gtgcggacag gtcgagctgg ctgagtttgt cgtaaaaata ggaaccgtcg
     4741 gacttcaggt agattttttc gttgcgcccg tatttgtagc gggcgttcag cactttgccc
     4801 tgtgcgggac ggtagcttgc accgacggcg tagttctcgg cgcgtttgtc gttttggttg
     4861 tagtggatgc tgctgtcgag gatgaagcgg ctgccgatgc tgccggaggc aaatgccacc
     4921 cagtcggaac ggttgcgcgg ttttttgccg acgctgccgt caagcatcac cgcatcatcc
     4981 ttgaaataga atttctgacc gatgccggcg cggaaacgct cttcccccgt cgcgccgtcc
     5041 aaaatacggc tttgcacggc ggcggaaagg ctgtttgcgg tgttaatcct gtcgttgccg
     5101 taatagaggt tttcgcgaaa gagctgcccg tagccgaagc tgctttccga cgaatcgaaa
     5161 ttgggcaggt cgttttggga tttggcagga atatagttgt agaacaggcg cggctcgagg
     5221 gtttgcagga ctccgccgcc gaacatccgc gtattccgct caaaagttgc gccgctgtcg
     5281 atgttgacaa tgggcagagt gcggctgacg cgtcgggctt cttggctgcc gaagcggttg
     5341 aggctgtaat aggtggcgtg cagtccgagt ttgggacgga cgtaacccca gctgttgctg
     5401 aaatcccatt tgatgtcggg atagacgacg aggcggctgc cgtcttggcg gctgtcgtgg
     5461 ctgaagcggg taaattgtgc ggacacgccg atttgcgccc tgccggtgtt tttgcgccaa
     5521 tcggcggaaa ggcgcggcat cagggcatac ggtttgtctt tgtagccgct ttggtttgcc
     5581 agcgtctggt atttcagaac cgaaaggccg gcattcaggc tgccgcccgc cgccctgccg
     5641 ccataatcca gccatacacg gcggttgagg ttgacgttgc cggcgatttc tttgttgccg
     5701 taaaagtcgc ggtagtagcc gctgtcggag acttggttga aatcgacacc cgcctgaagc
     5761 gtgtcggaaa tgtcgtgccg atgctgccat ttcgcctgat agcggttatt cctgccgctt
     5821 ttcttgtcgt gcggcagcca ggtcaggtcg gactggccgg cataatccgg ccgcaggtag
     5881 cgtacctgcc cgtcaaagac cgcgccgcgt tcgccgatca cgctgggcgc gaacgtggca
     5941 tcgagattgg gggcaaggtt gaaataatag ggaacggaaa gggaaacgcc gtccgaaccg
     6001 gcggacagtg agggaacaag caggccgctt ttgcggttgc cgtcaagcgg gaagtccgcc
     6061 caaggggtgt agaaaatggg aacgccgccg aacacgaagg cggcgtgttt ggcaacgcct
     6121 atgccttttt cccgatcggc ttcgacggaa gcggccttga cataccagcc ggcatcgccg
     6181 gcggaacagg tgttgaattg ggtttccgtc agtttgtaat gcccttcgcc caacatttcg
     6241 gcggtgcggc tgacgctttg cagccgccgt ccgccgtgtt cggtttccat acggacgttg
     6301 tgcgcttccc cggtctgctg ctcgagattg taggtcaggg tttcgccccg aatcagcgta
     6361 ccgtcctgtt ggagggcgaa ccggtcgcct gcggtaacgg tgtcgcccga ctggtcgtaa
     6421 tccgcccaat cggtattgag ggtcgtccgg ttgcgttcga cgacgacgtt gccttcggca
     6481 cgcacctgca cctgcgactg tccttccacc ttgtcggcaa caatgcgcgt atagtcttcg
     6541 gggatggatg cttcgccgct gcctcggacg gcggcttcgg ttctctcggg gctgccgctt
     6601 tcgttactgc aaaacaggca ggtcgaaccg aggctcaggc tggtaggctg tatgggttcg
     6661 gacacgctcc gaacgcttcc tccggcggtc ggattgtccg tttcctccgc cgcaacggca
     6721 tcggcggcgg cgcaatgcgt gccgaaacag aagcccaatg ccagcaccag tggtttgagt
     6781 gaaaataaac gagccaaaat cgcccctcaa gtcggtttgc cagttagaat agcgtttatt
     6841 gtaacctgaa atgctttagt actgttatgc aacggcaaac cgaactgaaa aattggcttc
     6901 agaccatcta tcccgaacgg gacttcgatc tgtccttcgc ggcggcggat gctgatttcc
     6961 gccgctattt ccgtgcaacg ttttcagacg gcagcagtgt cgtctgcatg gatgcaccgc
     7021 ccgacaagat gagtgtcgca ccttatttga aagtgcagaa actgtttgac atggtcaatg
     7081 taccgcaggt attgcacgcg gacacggatt tggggtttgt ggtattgaac gacttgggca
     7141 atacgacgtt tttgaccgcg atgcttcagg agcagggcga aacggcgcac aaagccctgc
     7201 ttttggaggc aatcggcgag ttggtcgaat tgcagaaggc gagccgtgaa ggggttttgc
     7261 ccgaatatga ccgtgaaacg atgttgcgcg aaatcaacct gttcccggaa tggtttgtcg
     7321 caaaagaatt ggggcgcgaa ttaacattca aacaacgcca actttggcag caaaccgtcg
     7381 atacgctgct gccgcccctg ttggcgcagc ccaaagtcta tgtgcaccgc gactttatcg
     7441 tccgcaacct gatgctgacg cgcggcaggc cgggcgtttt agacttccaa gacgcgcttt
     7501 acggcccgat ttcctacgat ttggtgtcgc tgttgcgcga tgcctttatc gaatgggaag
     7561 aagaatttgt cttggacttg gttatccgct actgggaaaa ggcgcgggct gccggcctgc
     7621 ccgtccccga agcgtttgac gagttttacc gctggttcga atggatgggc gtgcagcggc
     7681 acttgaaggt tgcaggcatc ttcgcacgcc tgtactaccg cgacggcaaa gacaaatacc
     7741 gtcccgaaat cccgcgtttc ctcaattacc tgcgccgcgt atcgcgccgt tatgccgaac
     7801 tcgccctgct ctacgcgctc ttggtcgaac tggtcggcga tgaagaactg gaaacgggct
     7861 ttacgtttta aacccaatca aaatgccgtc tgaaaaccaa gtttcagacg gcatttttca
     7921 aacgggctta ctgcgcggct ttttgttctt cgcgtacttt gtccgccaaa aggtcgatgg
     7981 tgttcatacc ggactcccag tcggcaaatt caactttata tttaccgccg acgataaccg
     8041 tgggcgtacc gtcgatttgg aaggtttcgg tcagctcctg cattttgtcg gcgcgcgcct
     8101 ggctttcggg ggactcgtag gcggcaagga cttttttgcc gtcaaaggcg gtttgttcgc
     8161 ccagccattt tttgaggact tccggctctt gcagcttgat tttttggttg accatcgcat
     8221 cgaaaatatg gctgttcgcc acatctttgc tgtcggcggc agccatatcg acggcggcgg
     8281 cgaggcgtgc cagcggcagc atttcttttt gccaaacgac atgctcggta cgcaggtaca
     8341 tatcgtcttt aaaagacttg gcgtgtttgc tcaaaacagg ttcgaggtgg gcgcagtgcg
     8401 gacagaaata gccgaaaaac tcaaggactt cgactttgcc tgcctgctgt tggggaatcg
     8461 ggttggcaag gacggtatag ttttgccctt cgaccagccc tgccggggcg gcggctgccg
     8521 aagcggcagg cgcgctgtcg gcggggacgc tggtttggac tttgctgtcg cacgcggcaa
     8581 gggcgaacag ggcggcaacg ccgagggcga gttgtctgga tttcatacgg ctctccgtga
     8641 tgttggaaac agatgttgga aatcaatcgg aatccggcta ttttattgca tttccgcgtg
     8701 ttgatacagt ttgccgccgg aaaaggacgt tttcgtttcg ggaaaccgct tcagacggca
     8761 tcaaacccga tgccgtctga agcggtttct gtcgtacaat acgcgccgtt gccccaaacg
     8821 ggtacgactg ttgaggaaca atgatgaata tgctgggagc tttggcaaaa gtcggcagcc
     8881 tgacgatggt gtcgcgcgtt ttgggatttg tgcgcgatac ggtcattgcg cgcgcattcg
     8941 gcgcgggcat ggcgacggat gcgttttttg tcgcgttcaa actgcccaac ctgcttcgcc
     9001 gcgtgtttgc ggagggggcg tttgcccaag cgtttgtgcc gattttggcg gaatataagg
     9061 aaacgcgttc gaaagaggcg acggaggctt ttatccgcca tgtggcgggg atgctgtcgt
     9121 ttgtgctggt catcgttacc gcgctgggca tacttgccgc gccttgggtg atttatgttt
     9181 ccgcacccgg ttttgccaaa gatgccgaca aatttcagct ctccatcgat ttgctgcgga
     9241 ttacgttccc ctatatctta ttgatttcac tttcctcttt tgtcggctcg gtactcaatt
     9301 cctatcataa attcagcatt ccggcgttta cgcccacgtt cctgaacgtg tcgtttatcg
     9361 tattcgcgct gtttttcgtg ccgtatttcg atcctcccgt taccgcgctg gcttgggcgg
     9421 tttttgtcgg cggcatttta caactcggct tccaactgcc ctggctggcg aaactgggtt
     9481 ttttgaaact gcccaaactg aatttcaaag atgcggcggt caaccgcgtc atgaaacaga
     9541 tggcgcctgc gattttgggc gtgagcgtgg cgcagatttc tttggtgatc aacacgattt
     9601 tcgcgtctta tctgcaatcg ggcagcgttt catggatgta ttacgccgac cgcatgatgg
     9661 agctgcccgg cggcgtgctg ggggcggcac tcggtacgat tttgctgccg actttgtcca
     9721 aacactcggc aaaccaagat acggaacagt tttccgccct gctcgactgg ggtttgcgcc
     9781 tgtgcatgct gctgacgctg ccggcggcgg tcggactggc ggtgttgtcg ttcccgctgg
     9841 tggcaacctt gtttatgtac cgagaattca cgctgtttga cgcgcagatg acgcaacacg
     9901 cgctgattgc ctattctttc ggtttaatcg gtttaatcat gattaaagtg ttggcgcccg
     9961 gcttttatgc gcggcaaaac atcaaaacgc ccgtcaaaat cgccatcttc acgctcattt
    10021 gcacgcagtt gatgaacctt gcctttatcg gcccactgaa acacgtcgga ctttcgcttg
    10081 ccatcggtct gggcgcgtgt atcaatgccg gattgttgtt ttacctgttg cgcagacacg
    10141 gtatttacca acctggcaag ggttgggcgg cgttcttggc gaaaatgctg ctctcgctcg
    10201 ccgtgatggg cggcggactg tgggcggcgc aggcttgcct gccgtttgaa tgggtacgcg
    10261 ccggcggaat gcggaaagcg gggcagctct gcatcctgat tgccgtcggc ggcggactgt
    10321 atttcgcatc actggcggct ttgggcttcc gtccgcgcca tttcaaacgc gtggaaaact
    10381 gaccgatgcc gatatccttt ttcggcaaac gcttttccac gcctatattc gggcgcgcat
    10441 cccccacgtc cgcaccagcc ggacgggctg ccgcatcatc aatgccgtct gaaaacaaga
    10501 aaaccgatac cttatgattt taacaccgcc ggacacgccc tttttcctcc gcaacggcaa
    10561 tgccgacacg attgccgcca aattcctgca acgccccgca cccacatacc gccgcgagct
    10621 gcttcccgac agcacgggta aaaccaaagt cgcctacgat ttttcagacg gcatttcgcc
    10681 cgatgcgccg ctggtcgtgc tgtttcacgg tttggaagga agcagccgca gccattacgc
    10741 ggtcgaactg atgctcgcgg tacgcagccg cggttggcac ggcgcagtcg tccatttccg
    10801 cagctgcggc ggcattgcca acaccgctcc ggtgttctac cacttgggcg ataccgccga
    10861 aatcgccttt actttggaca cgcttgccgc gcgttaccgt gaaatatacg ccgtcggcgt
    10921 atcactgggc ggcaacgcgc tggcaaaata tttgggcgaa cagggcgaaa acgcgctgcc
    10981 gcaagccgct gccgtcatct ccgcccccgt cgatgcagag gcggcaggca gacgcttcga
    11041 cagcggcatc acgcggctgc tctacacgcg ctacttcctc cgcaccctga tacccaaagc
    11101 acggacagtt ccgggttttc agacggcatt tgccgcaggg tgcaaaacac tgggcgagtt
    11161 tgacgaccgt ttcaccgcac cgctgcacgg ctttgccgac cggcacgact actaccgccg
    11221 aacttcctgc aaaccgctgc tcaaacacgt tgccaaaccg ctgctcctgc tcaacgccgc
    11281 caacgacccc ttcctgccgc ccgaagccct gccccgcaca gacgaagtgt ccgaagccgt
    11341 taccctgttc cagccggcac acggtggtca tgtcggcttt gtcggcagca ccggtggcag
    11401 gctgcacctg caatggctgc cgcagaccgt cctgtcctat ttcgacagct tccgcacaaa
    11461 cagtcgttaa cggtttgatg ctaatattcc ccctttccca gacgaatacg gagcccgaca
    11521 tgaccgacat cctcaataaa atccttgcca ccaaagcaca ggaagttgcc gcccaaaaag
    11581 ccgccgtcaa cgccgaacac atccgcgcac ttgccgaaaa cgcaccgccc gtccgcagct
    11641 tcatcgattc gatacgcggc aaacaccgtc taaacctgcc cgctgtcatt gccgaaatca
    11701 aaaaggcaag cccgagcaaa ggcctgatcc gtccggactt ccgaccggcc gagattgcac
    11761 gcgcctatga aaacgccgga gcggcgtgtt tgtccgtact gaccgacgaa ccctatttcc
    11821 aaggttcgcc cgaatacctc aaacaagcgc gcgaagccgt atcgctgccc gtgctgcgca
    11881 aagacttcat catcgacgaa taccaggttt atcaggcgcg cgcatggggg gcggatgccg
    11941 tcctgctgat tgccgcagca ctggaacagg aacaattgga acgctttgaa gcggtggcgc
    12001 acgaattggg catgaccgtc ctgctcgagc tgcacgatga aaccgaattg gaaaaatgcc
    12061 gcaatctgac cacgccgttg tggggcgtaa acaaccgcaa cctgcggact tttgaagtct
    12121 ccctcgacca aaccctgtcg ctgctgcccg cgctggaagg caaaaccgtc gttaccgaaa
    12181 gcggcattac gggtaaggcg gatgtggaat ttatgcgggc gcgcggcgtg cataccttcc
    12241 tgatcggcga aacgttcatg cgtgccgacg atattgagac ggaagtgggc aaactcttct
    12301 aaatcccgat ttcagacggc atattgccgc cgaccgacca acacccttac caagcacgga
    12361 accatcatgc accgaccgac cgccaaacag attttgcacg aagtattcgg ttatcccgaa
    12421 tttcgcggca ggcaggaggc tgtcatcaat actttggcag gcggcaggag tttgatggtg
    12481 ctgatgccga cgggcggggg caagtctttg tgttaccaga ttcccgcgct gatgcgcgaa
    12541 ggcgtggcgg ttgtcgtatc gccgctgatt gcgctgatga acgaccaagt ggccagcctg
    12601 catgtggccg ggattgaagc agcggcagtc aacagcggca catcggcaga tgaggcgcgc
    12661 gagattgccg acaagcttgc ccaaggccgt ctgaaactgc tttatgtcgc accggaacgc
    12721 ttggttaccg accgcttttt gcgttttctc gaccaacaaa ccgtcagtct gtttgccatt
    12781 gacgaggcgc attgtgtcag ccagtggggg cacgatttcc gccccgaata ccaacagctc
    12841 ggcatccttg ccgaacgcta tccgaacgtc ccgcgcatcg ccctgaccgc taccgccgat
    12901 gccgccacgc gcgccgacat caagcattat ctgcacttgg acaatgcgcc cgaatttgtc
    12961 tccagcttcg accgtccgaa tatttattat caggttatcg aaaaaaacaa cggcaaaaaa
    13021 caattgctgg atttcatccg caaagaaatg acggggcaaa gcggcattgt gtattgccta
    13081 agccgcaaaa aggttgaaga tgtggcgcag tttttgcgtg aaaacggatt aaacgcgatt
    13141 ccgtatcatg ctggtttgag catggacgtg cgcgaggaaa accaacgccg ctttacgcat
    13201 gaagacaata ttatcgtggt ggcgaccgtg gcgttcggca tgggcataga caaacccgac
    13261 gtgcgctttg tcgcccatct cgatatgccc cagagtgtcg aacatttcta tcaggaatcg
    13321 gggcgtgccg gcagggacgg gctgcctgcc gcgagctggc tgtgttacgg tttgaacgat
    13381 tgggtgttgc tgcgcgaacg gattgccgaa ggcaacagcg acgaggtgca aaagcaaatc
    13441 gaaatgcaaa aactcgatgc catgctttcc gtctgcgaaa ccgccgcctg ccgccgcgta
    13501 ctgctgctca aacatttcgg cgaagaatcc gaaccctgcg gccattgcga caactgcctg
    13561 catccgcccg tacggtttga cggcacggtg ttggtgcaaa aattactcag ctgcgtgtac
    13621 cgcgccggac aacgttttgc cgccggttac atcaccaacc ttttgcgcgg caaaagcgac
    13681 gattggatac gcggcaaccg gcacgaacaa ctgtccacct tcggcatcgg tacggagttg
    13741 tccgacaaag aatggcgcag cgtcatccgc cagtgtatca gcctcggcta cctcaccgtc
    13801 aacattgccc gatatcaggc attgcaactg accgaagccg ccaaaaaagt cctcaaaggc
    13861 gaaaccgaag tgatgctgcg tccgctcaag cgcgacaagc ccgccgcccg caccctcaaa
    13921 gacaactggc tgcgtaccga acgcgaagaa cgcctgtggc aggcattgcg cgtttggcgt
    13981 atgaaacagg cagaagccga agtcatcccc gcctatatga ttttcggcga caaaaccctg
    14041 cgcgaccttg tcgaaaaaat gccgcaagac ctcaacggac tgcacgacat ctacggtttg
    14101 ggcgaagcca aaatcgaccg tttcggacac ggcatcctcg aagtctgccg caacaccgcc
    14161 gactttagcc acaatgccgt catccgcccg caaaccgaac gcgaacaaca actgcgtcaa
    14221 aaactcgaag cctggcggta tgaacaggca agggcggaaa actgcgccct gcatgccgtc
    14281 ctctccgacg aaagccttgc cgatatgctt gccgccacgc ccgaaaccga aaccgacctc
    14341 gaaggcgtac acggcttggg cagcgtacgc gccgccaaat acggacggga catcctcgcc
    14401 gtctgccgtc cgttttcaga cggcatcgat gaaaccgcca aacacaaacg ccgcctgatg
    14461 cgcgccctga tccaatggtg caacgaaacg gcaaaacacg aacagtccga accctaccgc
    14521 attctcagca aagccgccct gcgcaccatt gccgccaaac agccggaagg tttggcggag
    14581 cttgccgccg tatacggcgt aggcgaagaa aaagccgcac gttacggtgc ggcggtgttg
    14641 gcggtgttgg aacggaatgc cgtctgaagc ccgttaccaa gtttcagacg gcattgcctc
    14701 tatttaaaaa ttcctgtttt tatagtg
//
LOCUS       DAXHSF010000034        13769 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.034, whole genome shotgun sequence.
ACCESSION   DAXHSF010000034 DAXHSF010000000
VERSION     DAXHSF010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13769
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.034"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(116..1441)
                     /locus_tag="ACFM8S_000793"
     CDS             complement(116..1441)
                     /locus_tag="ACFM8S_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFC6313892.1"
                     /translation="MKKYLFRAALCGIAAAILAACQSKSIQTFPQPDTSIINGPDRPV
                     GIPAPAGTTVAGGGAVYTVVPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCA
                     QAFQTPVHSFQAKQFFERYFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIY
                     GIPDDFISVPLPAGLRSGKALVRIRQTGKNSGTIDNAGGTHTADLSRFPITARTTAIK
                     GRFEGSRFLPYHTRNQINGGALDGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIR
                     IGYADKNEHPYVSIGRYMADKGYLKLGQTSMQGIKAYMQQNPQRLAEVLGQNPSYIFF
                     RELTGSSNDGPVGALGTPLMGEYAGAVDRHYITLGAPLFVATAHPVTRKALNRLIMAQ
                     DTGSAIKGAVRVDYFWGYGDEAGELASKQKTTGYVWQLLPNGMKPEYRP"
     gene            1653..2492
                     /gene="efeU"
                     /locus_tag="ACFM8S_000794"
     CDS             1653..2492
                     /gene="efeU"
                     /locus_tag="ACFM8S_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFC6313893.1"
                     /translation="MLVAFLIMLREGIEAALIVGIVAGFLKQSGHSKLMPKVWFGVVL
                     ASLMCLGLGYGIHSATGEIPQKQQEFVVGIIGLVAVAMLTYMILWMKKAARSMKRQLQ
                     DSVQAALNRGSGQGWALVGMAFLAVAREGLESVFFLLAVFKQSPTWQMPAGAVAGVLA
                     AAVIGALIYQGGMRLNLAKFFRWTGAFLIAVAAGLLAGSLRALHEAGIWNALQDIVFD
                     SSKYLHEDSPLGVLLGGFFGYTDHPTQGEALVWLLYLIPVITWFLCGSRPSETLTRKE
                     ELK"
     gene            2489..3655
                     /gene="efeO"
                     /locus_tag="ACFM8S_000795"
     CDS             2489..3655
                     /gene="efeO"
                     /locus_tag="ACFM8S_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFC6313894.1"
                     /translation="MRKFNLTALSVMLALGLTACQPPEAEKAAPAASGEAQTANEGGA
                     VSIAVNDNACEPMELTVPSGQVVFNIKNNSGRKLEWEILKGVMVVDERENIAPGLSDK
                     MTVTLLPGEYEMTCGLLTNPRGKLVVTDSGFKDTANEADLEKLSQPLADYKAYVQGEV
                     KELVAKTKTFTEAVKAGDIEKAKSLFADTRVHYERIEPIAELFSELDPVIDAREDDFK
                     DGAKDAGFTGFHRIEYALWVEKDVSGVKEIAAKLMTDVEALQKEIDALAFPPGKVVGG
                     ASELIEEVAGSKISGEEDRYSHTDLSDFQANVDGSKKIVDLFRPLIETKNKALLEKTD
                     TNFKQVNEILAKYRTKDGFETYDKLGEADRKALQASINALAEDLAQLRGILGLK"
     gene            3919..5184
                     /gene="efeB"
                     /locus_tag="ACFM8S_000796"
     CDS             3919..5184
                     /gene="efeB"
                     /locus_tag="ACFM8S_000796"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFC6313895.1"
                     /translation="MSKNQPAQPTRRTLFKTAIAAGAVGAIGGYLGGKKRGETAERTA
                     ESQHSPQAYPCYGEHQAGIVTPQQAFSIMCAFDVTAQSAKQLENLFRTLTARIEFLTQ
                     GGEYQDGDDKLPPAGSGILGKAFNPDGLTVTVGVGSSLFDGRFGLKDKKPIHLQEMRD
                     FSNDKLQKSWCDGDLSLQICAFTPETCQAALRDIIKHTVQTAVIRWSIDGWQPKSEPG
                     AMAARNLLGFRDGTGNPKVSDPKTADEVLWTGVAANSLDEPEWAKNGSYQAVRLIRHF
                     VEFWDRTPLQEQTDIFGRRKYSGAPMDGKKEADQPDFAKDPEGNTTPKDSHIRLANPR
                     DPEFLKKHRLFRRAYSYSRGLASSGQLDVGLVFVCYQANLADGFIFVQNLLNGEPLEE
                     YISPFGGGYFFVLPGVEKGGFLGQGLLGV"
     gene            complement(5256..5585)
                     /locus_tag="ACFM8S_000797"
     CDS             complement(5256..5585)
                     /locus_tag="ACFM8S_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFC6313896.1"
                     /translation="MSLPPCPQCASEYTYEDGGQYICPECAHEWNETESAADLAAQVL
                     DANGAVLQNGDTVILIKDLKVKGSSMVIKQGTKVKGIRLQEGDHNIGCKIDGSAMNLK
                     SEFVKKA"
     gene            complement(5662..7032)
                     /gene="glmU"
                     /locus_tag="ACFM8S_000798"
     CDS             complement(5662..7032)
                     /gene="glmU"
                     /locus_tag="ACFM8S_000798"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFC6313897.1"
                     /translation="MPQNTLNIVILAAGKGTRMYSKMPKVLHEIGGETMLGRVIDTAA
                     ALNPQNICVVVGHGKEQVLDTVKRDVVWVEQTEQLGTGHAVKTALPHLAAEGRTLVLY
                     GDVPLIDVETLETLLEAAGNEVGLLTDVPADPSGLGRIIRDGSGSVTAIVEEKDASAA
                     QKAVKETNTGILVLPNAKLENWLNSLSSNNAQGEYYLTDLIAKAVADGIKVHPVRVRA
                     SHLAAGVNNKRQLAELERIFQTEQAQELLKAGVTLRDPARFDLRGRLKHGQDVVIDVN
                     CIFEGEVEIGDNVEIGANCVIKNAKIGANSKIAPFSHLEDCEVGENNQIGPYARLRPQ
                     AKLANDVHVGNFVEIKNAAIGKGTKANHLTYIGDAEVGSKTNFGAGTIIANYDGVHKH
                     KTVIGDEVRIGSNCVLVSPVKIGNKVTTGAGSTITRNVEDGKLALARSRQTIIDGWVR
                     PEKNKQ"
     gene            complement(7087..7359)
                     /locus_tag="ACFM8S_000799"
     CDS             complement(7087..7359)
                     /locus_tag="ACFM8S_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex protein"
                     /protein_id="HFC6313898.1"
                     /translation="MRKTFLILMTAAALLSGCAWETYQDGNGKTAVRQKYPAGTPVYY
                     QDGSYSKNMNYNQYRPERHAVLPNQTGNNADEEHRQHWQKPKFQNR"
     gene            complement(7335..8066)
                     /locus_tag="ACFM8S_000800"
     CDS             complement(7335..8066)
                     /locus_tag="ACFM8S_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFC6313899.1"
                     /translation="MQENPTVWLFDLDNTLHNADAGIFTLINRAMTRYMARRLKLSES
                     AASDLRQDYWHRYGATLAGLQIHHPEIDIAEFLRESHPIDAILTRLHGMADTENTLCR
                     LNGRKAVFSNGPSFYVRAVVGALGLENRFDALFGTDDFGLLYKPNPQAYLNVCRLLDV
                     PPECCIMVDDSADNLHQAKALGMKTVRFGAKVHDLPFIDASVSDMAQLARYAETLSEH
                     RQNHYNTRTPPRKYERMLCVKPSLS"
     gene            8125..9126
                     /locus_tag="ACFM8S_000801"
     CDS             8125..9126
                     /locus_tag="ACFM8S_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6313900.1"
                     /translation="MKRKIWLLPLLAVSAYLQAQTEVRLAVHKSFSLPKGLIARFERA
                     NDAKVSIIQAGGANEMLNKLILSRANPIADAVYGLDNANIGKAREMGILAAAQPESAP
                     VAVGLPSALAVDYGYVSINYDKKWFEGKKLPLPQTLQDLTRPEYKNLLVVPSPATSSP
                     GLGFLMANISGLGEEGAFKWWAQMRQNGVKVAKGWSEAYYTDFSHNGGAYPLVVGYAA
                     SPAAEVYFSKGKYSEPPTGNLFLKGGVFRQVEGAAVLKGAKQPKLAAKLVQWLQSREV
                     QQAVPSEMWVYPAVKNTRLPDVFRFAQAPTHTTAPAQRDIDANQRGWVSRWIRTVLK"
     gene            9220..10068
                     /locus_tag="ACFM8S_000802"
     CDS             9220..10068
                     /locus_tag="ACFM8S_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFC6313901.1"
                     /translation="MDFKQFDFLHLISVSGWEHLAEKAWAFGLNLAAALLIFLVGKWA
                     AKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGG
                     AGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
                     EEVVLPNSVVMGNSIVNRSTLPLCRAQVIVGVDYNCDLKVAKEAVLKAAVEHPLSVQN
                     EERQAAAYITALGDNAIEITLWAWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRD
                     IHIINS"
     gene            10094..10579
                     /locus_tag="ACFM8S_000803"
     CDS             10094..10579
                     /locus_tag="ACFM8S_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HFC6313902.1"
                     /translation="MDALHTIARNLTKKRQTVSCAESCTGGLLAAVFTSVAGSSQWFD
                     QSFVTYSNKAKEDRLGVLPETLLEHGAVSRQTVYEMARGAKAVAQADYAVGISGIAGP
                     GGGSESKPVGTVWFGFAFPGGSCEAMRRFDGNRESVRAQAVAFALERLAGLIENGGDA
                     V"
     gene            complement(10681..12249)
                     /gene="msrAB"
                     /locus_tag="ACFM8S_000804"
     CDS             complement(10681..12249)
                     /gene="msrAB"
                     /locus_tag="ACFM8S_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HFC6313903.1"
                     /translation="MKHRTFFSLCAKFGCLLALGACSPKIVDAGAATVPHTLSTLKTA
                     DNRPASVYLKKDKPTLIKFWASWCPLCLSELGQTEKWAQDAKFSSANLITVASPGFLH
                     EKKDGDFQKWYAGLNYPKLPVVTDNGGTIAQSLNISVYPSWALIGKDGDVQRIVKGSI
                     NEAQALALIRDPNADLGSLKHSFYKPDTQKKDSKIMNTRTIYLAGGCFWGLEAYFQRI
                     DGVVDAVSGYANGNTKNPSYEDVSYRHTGHAETVKVTYDADKLSLDDILQYFFRVVDP
                     TSLNKQGNDTGTQYRSGVYYTDPAEKAVIAAALKREQQKYQLPLVVENEPLKNFYDAE
                     EYHQDYLIKNPNGYCHIDIRKADEPLPGKTKTAPQGKGFDAATYKKPSDAELKRTLTE
                     EQYQVTQNSATEYAFSHEYDHLFKPGIYVDVVSGEPLFSSADKYDSGCGWPSFTRPID
                     AKSVTEHDDFSYNMRRTEVRSHAADSHLGHVFPDGPRDKGGLRYCINGASLKFIPLEQ
                     MDAAGYGALKSKVK"
     gene            12394..13659
                     /gene="ftsY"
                     /locus_tag="ACFM8S_000805"
     CDS             12394..13659
                     /gene="ftsY"
                     /locus_tag="ACFM8S_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFC6313904.1"
                     /translation="MFSFFRRKKKQETPAPEEAQVQETAAKAESEVTQIVENIKEEVE
                     SLTESVKGQVESAVETVSGAVEQVKETVAEMLSEAEEAAEKAAEQVEAAKEAVAETVG
                     EAVGQVQEAVATTEEHKLGWAARLKQGLTKSRDKMAKSLAGVFGGGQIDEDLYEELET
                     VLITSDMGMEATEYLMKDVRDRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPET
                     KEPFVIMLAGINGAGKTTSIGKLAKYFQAQGKSVLLAAGDTFRAAAREQLQAWGERNN
                     VTVISQTTGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHLMEEIKKVKRVLQKA
                     MPDAPHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRP
                     VPVRYIGVGEGIDDLRPFDARAFVDALLD"
BASE COUNT         2952 a         3537 c         4124 g         3156 t
ORIGIN      
        1 atccactata attccgtcaa ataagaaagg aattttgtgc ctgcggtatc gcaaaacttc
       61 gccttaatgc gcccgattga ctagggatgg gcttcagatg gcattgtttt ccggtttacg
      121 ggcggtattc gggcttcata ccgttgggca gaagctgcca gacatatccc gtggttttct
      181 gtttgctggc aagttcgccg gcttcgtcgc cgtatcccca aaaataatcc acgcgcaccg
      241 cgcctttaat cgcgctgccg gtatcctgcg ccataatcag gcggttgagg gctttgcggg
      301 taaccggatg ggcggtggcg acaaataagg gcgcgcccaa ggtaatgtag tgccggtcga
      361 ctgcgccggc gtactcgccc atcagcggcg tgcccagtgc gccgacaggg ccgtcattgc
      421 tgcttccggt aagctctcgg aaaaagatat agctggggtt ttgccccaaa acttcggcga
      481 ggcgttgcgg gttttgctgc atataggctt tgatgccctg catggaggtt tgtccgagtt
      541 tgaggtagcc cttatccgcc atatagcgtc cgatggaaac gtagggatgt tcgtttttgt
      601 cggcatagcc gatgcggatg tatttgccgg acggggtttt cagacggccc gagccttgga
      661 tgtgcataaa aaaaagttcg acagggtctt cggcgtaacc gagtatcggt gctttgccgt
      721 caagcgcgcc gccgttgatt tggttgcgcg tgtggtaggg gaggaagcgg cttccttcaa
      781 acctgccttt gattgccgtt gtgcgcgcgg tgatggggaa tcgggagagg tcggcggtgt
      841 gcgtgccgcc ggcattgtcg attgtgccgc tgtttttccc cgtctgcctg atgcggacaa
      901 gggcttttcc gctccgcaaa ccggcaggca gggggacgga gataaaatcg tcgggaatac
      961 cgtaaatcgg gaagcgggct tgtgccgtcc gcctgtcgtc gcccttcagc accggctcgt
     1021 aatagccggt aaccgtaccg gcaaggcttc cgttgcctgc aacctgccac ggcgtgaaat
     1081 agcgttcaaa aaactgtttt gcctgaaagg aatggacggg ggtttgaaag gcttgggcac
     1141 acacatcctg ccagccttgg cggtttttca aattggcgca gccgaggcgg aaggattgca
     1201 ggcttttggc gaaatcctgc gccgcccagt ggggcaggga caggtgcggc acaacggtat
     1261 aaacggcccc gccgccggca accgtcgttc cggcgggggc ggggatgccg accggccggt
     1321 ccgggccgtt gatgatggat gtgtcgggtt gcggaaaggt ttggatgctc ttgctttggc
     1381 aggcggcgag gatggcggcg gcgatgccgc acagggcggc gcggaacagg tattttttca
     1441 taatggacaa tgttgtcagc agtaataaaa aaagatggtt tcgggcggcg ttgcggcagc
     1501 cgtggagagg ggattttaac acagggcgca gctgcagcct gcggaacttt ccgccgcgcg
     1561 gtactgcaga taaaaataac ttgcatttgt atttacaagc aatgaaaata ttccgataat
     1621 atattattca tcatccttgt tcgttcgcgt ttatgctggt cgctttttta attatgttgc
     1681 gcgagggtat cgaagccgcg ctcattgtcg gcatcgttgc cggttttctg aaacagtccg
     1741 gacattccaa actgatgcct aaggtctggt tcggggtcgt ccttgcttct ttgatgtgtt
     1801 tggggctggg gtacggaatc cattcggcaa cgggcgagat tccccaaaag cagcaggagt
     1861 tcgtcgtcgg cattatcggc ctggttgctg tcgcgatgct gacttatatg attttatgga
     1921 tgaaaaaagc cgcccgttcg atgaagcggc agcttcagga ttctgtgcag gcggctttga
     1981 accgtggcag cggtcaagga tgggccttgg tcggtatggc gtttcttgcc gtggcgcgcg
     2041 aaggtctgga gagcgttttt ttcctgcttg ccgtattcaa acagagcccg acgtggcaga
     2101 tgccggccgg tgcggtagcg ggggttttgg ctgccgccgt gattggcgcg ttgatttatc
     2161 agggcgggat gcgcctgaat ctggcgaagt ttttccgttg gacgggggcg tttttgattg
     2221 ccgttgccgc cggtctgctt gccggctcgc tgcgcgcgct gcatgaggca ggtatttgga
     2281 acgcgcttca ggacattgtg ttcgactcat caaaatattt gcacgaagac agcccgttgg
     2341 gcgtgctgct cggcggattt ttcggctata ccgaccatcc gacgcagggc gaggccttgg
     2401 tttggctgct gtaccttatt cccgtcataa cttggttttt gtgcggcagc aggccgtctg
     2461 aaactttaac ccgtaaagag gagctgaaat gagaaaattc aatttgaccg cattgtccgt
     2521 gatgcttgcc ttaggtttga ccgcgtgcca gccgccggag gcggagaaag ctgcgccggc
     2581 agcgtccggt gaggcgcaaa ccgccaacga gggcggtgcg gtcagtatcg ccgtcaacga
     2641 caatgcctgc gaaccgatgg aactgaccgt gccgagcgga caggttgtgt tcaatattaa
     2701 aaacaacagc ggccgcaagc tcgaatggga aatcctgaaa ggcgtgatgg tggtggacga
     2761 gcgcgaaaac atcgcccccg gactttccga taaaatgacc gtcaccctgt tgccgggcga
     2821 atacgaaatg acttgcggtc ttttgaccaa tccgcgcggc aagctggtgg taaccgacag
     2881 cggctttaaa gacaccgcca acgaagcgga tttggaaaaa ctgtcccaac cgctcgccga
     2941 ctataaagcc tacgttcaag gcgaggttaa agagctggtg gcgaaaacca aaacctttac
     3001 cgaagccgtc aaagcaggcg acattgaaaa ggcgaaatcc ctgtttgccg acacccgcgt
     3061 ccattacgaa cgcatcgaac cgattgccga gcttttcagc gaactcgacc ccgtcatcga
     3121 tgcgcgtgaa gacgacttca aagacggcgc gaaagatgcc ggatttaccg gcttccaccg
     3181 tatcgaatac gccctttggg tggaaaaaga cgtgtccggc gtgaaggaaa ttgcagcgaa
     3241 actgatgacc gatgtcgaag ccctgcaaaa agaaatcgac gcattggcgt ttcctccggg
     3301 caaggtggtc ggcggcgcgt ccgaactgat tgaagaagtg gcgggcagta aaatcagcgg
     3361 cgaagaagac cggtacagcc acaccgattt gagcgacttc caagccaatg tggacggatc
     3421 taaaaaaatc gtcgatttgt tccgtccgtt gatcgagacc aaaaacaaag ccttgttgga
     3481 aaaaaccgat accaacttca aacaggtcaa cgaaattctg gcgaaatacc ggactaaaga
     3541 cggttttgaa acctacgaca agctgggcga agccgaccgc aaagcgttac aggcctctat
     3601 taacgcgctt gccgaagacc ttgcccaact tcgcggcata ctcggcttga aataagccgc
     3661 aagcgttcag acggtatttg gcggcagata ccgtctgaag ttttatagtg gattaacaaa
     3721 aaccagtacg gcattgcctc gccttgcctt gccgtactat ttatactgtc tgcggcttcg
     3781 tcgccttgtc ctgatttttg ttaatccact atatccgcca tatattgcag ggcgggattt
     3841 caacctgccg ctatcggtta atggaaaaac ggcgtgcagg gatacccatc ctgctgcacg
     3901 gatattgaag gaaacaccat gagcaaaaac caacccgcac aaccgaccag gcgcactctt
     3961 tttaaaaccg cgatcgcagc cggagcagtc ggcgcaatcg gaggttatct cggcggcaaa
     4021 aaacggggcg aaaccgccga acgcaccgcc gaaagtcaac actcgcccca agcctatccc
     4081 tgctacggcg aacatcaggc aggcatcgtt acgccgcagc aggcgttttc gattatgtgc
     4141 gccttcgacg taaccgcgca aagtgccaag cagctggaaa acctgttccg cacgctgacc
     4201 gcccgcatcg agtttctcac ccaaggcggc gaataccaag acggcgacga caaacttccg
     4261 ccagccggca gcggcatttt gggcaaagcc ttcaaccccg acgggttgac cgttaccgtg
     4321 ggggtgggca gcagcctgtt tgacggccgg ttcggactca aagacaaaaa accgattcat
     4381 ttgcaggaaa tgcgcgactt ctccaacgat aagctgcaaa aaagctggtg cgacggcgat
     4441 ttgagcctgc aaatctgtgc cttcaccccc gaaacctgcc aagccgccct gcgcgacatc
     4501 atcaaacaca ccgtccaaac cgccgttatc cgctggagta tcgacgggtg gcagcctaaa
     4561 tccgaacccg gcgcgatggc ggcgcgcaac ctgttgggct tccgcgacgg cacgggcaac
     4621 cccaaagttt ccgaccccaa aaccgccgac gaggttttgt ggacgggtgt ggccgccaac
     4681 agcctcgacg aaccggagtg ggcgaaaaac ggcagctatc aggcagtccg ccttatccgc
     4741 cactttgtcg agttttggga caggacgccg cttcaagagc aaaccgacat tttcgggcgg
     4801 cgcaaataca gcggtgcgcc gatggacggc aaaaaagaag ccgaccaacc ggattttgcc
     4861 aaagaccccg aggggaatac cacgcccaaa gacagccata tacgcctggc gaatccgcgc
     4921 gatcccgaat tcctcaaaaa acaccgcctc ttccgccgcg cctacagcta ttcgcgcgga
     4981 ctcgcctcaa gcggacagct tgatgtcggg ctggtgttcg tctgctatca ggcaaacctt
     5041 gccgacggat tcatcttcgt gcaaaacctc ctcaacggcg aaccgctgga agaatacatc
     5101 agccccttcg gcggcggcta tttcttcgtc ttgcccggcg tggaaaaagg cggctttttg
     5161 gggcaagggc tgctgggcgt ataaatccgc catataaaaa acgccgtccg aaccttgcca
     5221 aacgggttcg gacggcgttt cttgtttttg ggcggtcagg cttttttgac gaattcggat
     5281 tttaaattca tcgcgctgcc gtcgattttg cagccgatgt tgtgatcgcc ttcctgcagg
     5341 cgtatgcctt tgacttttgt gccttgtttg atcaccatcg agctgccttt taccttgagg
     5401 tctttgatga ggatgacggt atcgccgttt tgcagcactg cgccgttggc atcgagcact
     5461 tgagccgcaa ggtcggcggc ggattcggtt tcattccatt cgtgggcgca ttcggggcag
     5521 atgtattgtc cgccgtcttc ataagtgtat tcggaggcgc attgtgggca tgggggtaat
     5581 gacatggttt gcagtcctta tctgatatcg gaggagtttg atgccgtctg aaacctgaaa
     5641 ccggcttcag acggcatagc tttattgttt gtttttttca ggcctcaccc agccgtcgat
     5701 gatggtttgg cgggagcggg cgagggcgag tttgccgtct tcgacattgc gggtaatcgt
     5761 gctgcccgcg ccggtggtta ctttgttgcc gattttgacg ggggagacca agacgcagtt
     5821 tgaaccgatg cgcacttcgt cgccgatgac ggttttgtgt ttgtgcacgc cgtcgtagtt
     5881 ggcaataatc gtacctgcgc cgaagttggt tttgctgccg acttcggcat cgccgatgta
     5941 ggtgaggtgg ttggctttgg tgcctttgcc gatggcggcg tttttgattt cgacgaagtt
     6001 gccgacgtgt acgtcgttag ctagtttggc ttgcggacgc aaacgggcgt atgggccgat
     6061 ttggttgttt tcgccgactt cgcagtcttc gaggtgggag aagggggcga ttttgctgtt
     6121 tgcgccgatt ttggcgtttt tgatgacgca gtttgcgccg atttcgacgt tgtcgccgat
     6181 ttcgacttcg ccttcaaaga tacagttcac atcaattacg acatcctgtc cgtgtttcag
     6241 acggcctcgt aaatcgaaac gtgccggatc gcgcagggtt acgcctgctt tgagcaattc
     6301 ttgtgcctgt tcggtttgga agatgcgttc gagttcggcg agctggcgtt tgttgttcac
     6361 gccggcggcg aggtgggagg cgcgcactcg gacgggatga actttaatac cgtcggcaac
     6421 ggctttggcg atgaggtcgg ttaaataata ttcgccttgg gcattgttgc tggaaaggct
     6481 gttcagccag ttttccagtt tggcgttggg caggacgaga atgcctgtgt tggtttcttt
     6541 cacggctttt tgggcggcac ttgcgtcttt ttcttcgaca atggcggtta cgctgccgct
     6601 gccgtcgcgg atgatgcgcc ccaaacctga cgggtcggcg ggcacgtcgg tcaacagccc
     6661 gacttcgttg cctgcggctt cgagcagggt ttcgagggtt tcaacgtcaa ttaaaggaac
     6721 gtcgccgtac aacaccagcg tgcggccttc ggcggcaagg tggggcaggg cggttttgac
     6781 ggcgtggccg gtgccgagct gttcggtttg ttcaacccag acgacatcgc gtttgacggt
     6841 gtccaaaact tgctctttgc cgtggccgac gacgacgcag atgttttgcg gattcagcgc
     6901 ggcggcggtg tcgataacgc gcccgagcat tgtttcgccg ccgatttcgt gcaaaacctt
     6961 gggcattttg gaatacatgc gcgtgccttt gccggcggcg aggatgacga tgtttaaagt
     7021 gttttgcggc atagcgtttt cctgtgcaat gccgtctgaa agggcttcag acggcatagg
     7081 gtgggtttat cggttttgaa atttgggctt ttgccagtgt tggcgatgct cttcgtcggc
     7141 gttgttgccg gtttggttgg gtaacacggc atggcgttcg ggacggtatt ggttgtagtt
     7201 catatttttc gagtagctgc cgtcttggta ataaacgggc gtgccggcgg gatatttttg
     7261 acggacggcg gtcttgccgt tgccgtcttg ataagtttcc cacgcgcagc ccgacaaaag
     7321 ggcggcggca gtcatcagga taaggaaggt tttacgcata acattctttc gtattttcgg
     7381 gggggggtgc gggtattgta atgattttgg cggtgttctg acaaagtttc tgcataccga
     7441 gccagttgcg ccatatcgct tacggaagca tcgataaagg gcaggtcgtg cacttttgca
     7501 ccgaaccgga cggttttcat gcccaatgct tttgcctgat gcaggttgtc cgcgctgtcg
     7561 tccaccataa tgcagcattc gggcggtacg tccaaaaggc ggcagacatt gagatacgct
     7621 tgcggattgg gtttgtacag cagcccgaaa tcatccgtgc cgaacagtgc gtcgaaacgg
     7681 ttttccaaac cgagtgcgcc gacaacggca cggacgtaaa acgacgggcc gttggaaaaa
     7741 accgccttgc gcccgttcag acggcatagg gtgttttccg tatccgccat gccgtgcagc
     7801 ctggtcagga ttgcatcgat cggatggctt tcgcgcaaaa attcggcgat gtcgatttcg
     7861 ggatggtgga tttgcagtcc ggcgagcgtt gcgccgtagc ggtgccaata gtcttgacgc
     7921 aggtcggacg cggcagattc ggagagtttg aggcggcgtg ccatatagcg tgtcatggcg
     7981 cggttgatga gtgtgaagat gccggcatcg gcattgtgca gcgtgttgtc gaggtcgaac
     8041 agccacacgg tcgggttttc ttgcattttg aaccgtgaaa atttgttaga atgttatttt
     8101 acagcgaata gaggaggact cggaatgaaa cggaaaattt ggctgctgcc gctgctggcg
     8161 gtttcggcat acctgcaggc gcagacggaa gtcaggctgg cggtgcataa gtcgttcagc
     8221 ctgcccaaag ggctgattgc gcgcttcgag cgggcaaacg atgcgaaggt gtcgattatt
     8281 caggcgggcg gcgcgaacga aatgctcaac aaactgattt tgagccgcgc caacccgatt
     8341 gccgacgcgg tgtatggttt ggacaacgcc aatatcggca aggcgcggga aatgggcatt
     8401 ttggcggcgg cgcaacccga atccgccccc gtcgcggtcg ggctgccttc ggctttggcg
     8461 gtcgattacg gctatgtgtc catcaattac gacaaaaaat ggtttgaagg caaaaagctg
     8521 cccctgccgc aaaccctgca ggatttgacc cgccccgaat ataaaaacct attggtcgtg
     8581 ccgtcccccg ccacgtcgtc cccggggctg ggcttcctga tggcgaacat cagcggtctg
     8641 ggcgaagaag gcgcgttcaa atggtgggca cagatgcggc agaacggcgt gaaggtcgcc
     8701 aaaggctgga gcgaggcgta ttacaccgac ttttcgcaca acggcggcgc gtatccgctg
     8761 gtggtcggtt atgccgccag cccggcggcg gaagtgtatt tttccaaagg caaatacagc
     8821 gagccgccga cgggcaacct gtttttaaaa ggcggcgtat tccgccaggt cgaaggcgcg
     8881 gcggtcttga agggcgcgaa acagccgaag ctggcggcaa aactggtgca atggctgcaa
     8941 agtcgggaag tgcagcaggc ggttccgtcc gaaatgtggg tttaccccgc cgtcaaaaac
     9001 acgcgcctgc ccgacgtgtt ccgcttcgcc caagccccga cgcacaccac cgcccccgcg
     9061 cagcgcgata ttgatgcgaa ccagcgcgga tgggtttccc gttggattag aacggttttg
     9121 aaataaaaca aacataccgc cccgcagggc ttcatacggc atttttacac ctgtgccgat
     9181 tacgccgcat ggggcggatg ttcgatcaag aggaaaacaa tggacttcaa acaatttgat
     9241 tttttacacc tgatcagtgt ttccggttgg gagcatctgg ctgaaaaggc gtgggcgttc
     9301 gggctgaacc ttgccgccgc gctgcttatt tttttggtcg gaaaatgggc ggcgaaacgc
     9361 attgtcgccg tgatgagggc ggcgatgacg cgcgcgcagg tcgatgccac gctgattagt
     9421 tttttgtgta atgttgccaa tatcggctta ttgattttgg tgattattgc cgcattgggc
     9481 agattgggcg tttccacaac atccgtaacc gccttaatcg gcggcgcggg tttggcggtg
     9541 gcgttgtccc tgaaagacca gctgtccaat tttgccgccg gcgcgctgat tatcctgttc
     9601 cgcccgttca aagtcggcga ttttatccgc gtcggcggtt ttgaaggata tgtccgagag
     9661 attaaaatgg tgcagacttc tttgcggacg accgacaacg aagaagtcgt gctgcccaac
     9721 agcgtggtga tgggcaacag catcgtcaac cgttccacac tgccgctgtg ccgcgcccaa
     9781 gtgatagtcg gcgtcgatta caactgcgat ttgaaagtgg cgaaagaggc ggtgttgaaa
     9841 gccgccgtcg aacacccctt gagcgttcaa aacgaagagc ggcaggccgc cgcctacatc
     9901 accgccttgg gcgacaatgc catcgaaatc acattatggg cttgggcaaa cgaagcagac
     9961 cgctggacgc tgcaatgcga cttgaacgaa caagtggtcg aaaacctccg caaagtcaat
    10021 atcaacatcc cgttcccgca acgcgacata cacatcatca attcttaaac gccgtctgaa
    10081 agaggagtgg gaaatggacg cgctgcacac catcgcccga aacctgacga aaaaacgtca
    10141 aaccgtaagc tgtgccgaat cctgcacggg tgggctgctt gccgccgtat tcacaagcgt
    10201 tgcaggcagt tcgcaatggt tcgatcaaag ttttgtaaca tacagcaaca aagccaaaga
    10261 agaccgcttg ggcgtgttgc ccgaaaccct gctcgaacac ggcgcggtca gccgccaaac
    10321 cgtctatgag atggcgcgcg gcgcgaaagc cgtggcgcag gcggattacg ccgtcggtat
    10381 ttccggcatc gccggtccgg gcggcggcag cgaaagcaaa cccgtcggca cggtttggtt
    10441 cgggtttgcc tttccgggcg gaagttgcga agcaatgcgc cgttttgacg gcaaccgcga
    10501 atccgtccgc gcgcaggcgg tcgccttcgc gttggaacgg ttggcggggc tgattgaaaa
    10561 cggcggcgat gctgtctaaa caaaatctcc gtctggacag aatccccgcc ggataaaaaa
    10621 taccgtctga aacgttgcag gtttcagacg gtattttgtc gggttaggcg gcggtgcggc
    10681 ttatttcact ttactcttca acgcgccgta gcctgccgcg tccatttgtt ccagcgggat
    10741 gaatttcaag ctcgcgccgt tgatgcagta gcgcagtccg cctttgtcgc gcgggccgtc
    10801 ggggaagacg tgtcccaaat gcgaatcggc ggcgtggctg cgcacttcgg tgcggcgcat
    10861 gttgtagctg aaatcatcgt gttcggtaac ggattttgca tcaatcgggc gcgtgaagct
    10921 cggccagccg cagccggaat catatttgtc ggcggagctg aacaaaggtt cgccgctgac
    10981 aacgtccaca taaatgccgg gtttgaacaa atggtcgtat tcgtggctga aggcatattc
    11041 ggtcgcgctg ttttgggtaa cttggtattg ctcttcggtc agggtgcgtt tgagttcggc
    11101 gtcactcggt tttttatacg ttgccgcatc gaagcctttg ccttgcgggg cggtcttggt
    11161 tttgcccggc agcggttcgt cggctttgcg gatatcgatg tggcagtagc cgttggggtt
    11221 tttaatcagg tagtcctgat ggtattcctc ggcatcgtag aagtttttca gcggctcgtt
    11281 ttcaacaacg aggggcagtt ggtatttttg ctgctcgcgt ttgagggcgg cggcgatgac
    11341 ggctttttcg gcggggtcgg tgtagtacac gccgctgcgg tattgcgtac cggtgtcgtt
    11401 gccctgtttg ttgaggctgg tcggatcaac gacgcggaag aaatattgca ggatgtcgtc
    11461 taggctgagt ttgtcggcat cgtaggtcac tttgacggtt tcggcgtggc ccgtatggcg
    11521 gtaggacacg tcttcatagc tcggattttt cgtgttgccg ttggcgtagc cggataccgc
    11581 gtcaaccacg ccgtcgatgc gttggaaata ggcttccaag ccccagaagc agccgccggc
    11641 gaggtaaatg gtgcgcgtgt tcatgatttt tgaatccttt ttctgagtgt cgggtttgta
    11701 gaacgaatgt ttcaagctgc ccaaatcggc attcgggtcg cggattaacg ccaacgcctg
    11761 cgcttcgttg atgctgcctt tgacgatgcg ctgcacgtcg ccgtctttac cgattaacgc
    11821 ccacgaaggg taaacgctga tattcaggct ttgggcgatc gtgccgccgt tgtcggttac
    11881 gacgggcagc ttgggataat tcaaaccggc ataccatttt tggaagtcgc cgtctttttt
    11941 ctcgtgcaaa aagcccggag acgcgacggt aatcaggttg gcggagctga attttgcatc
    12001 ttgcgcccat ttttcggtct gtcccaattc ggacaaacac aaaggacacc agctcgccca
    12061 aaatttaatc agcgtcggtt tgtctttttt caagtaaaca ctggcggggc ggttgtccgc
    12121 agttttcaaa gtggataaag tgtgcggcac ggtcgcggct ccggcatcga cgattttggg
    12181 cgaacaagca cccagcgcaa gcaggcagcc gaacttggcg caaagggaaa agaaagtacg
    12241 gtgtttcatt ttgagtttcc tgtgtggacg gtttgcatga ttagacgttt gagatgccga
    12301 aaccttacag ctcggatttt cagacaacct tgccgcgtaa aatacgctac aatacgccct
    12361 gtttcaagtt tctaaaatta aaaggaaaat tcaatgttca gcttcttccg tcgcaagaaa
    12421 aaacaggaaa cgccggctcc cgaggaggct caagttcagg aaactgcagc aaaagcagaa
    12481 tccgaagtta ctcaaatagt tgaaaatatt aaagaagaag tcgaatcttt aacggaaagc
    12541 gtcaaagggc aggtcgaatc ggctgttgaa accgtcagcg gtgcggttga acaggtaaag
    12601 gaaaccgttg ccgagatgct gtctgaagca gaggaagcgg cggaaaaagc agcggaacaa
    12661 gtcgaagcgg caaaagaagc cgttgccgaa accgtcggcg aggctgtcgg gcaagttcaa
    12721 gaagccgttg cgacaactga agaacacaag ctcggttggg cggcgcgttt gaaacaaggt
    12781 ttgaccaaat cgcgcgacaa aatggcgaaa tcgctggcgg gcgtgttcgg cggcggacaa
    12841 atcgacgaag atttgtacga agagctggaa accgtgctga ttaccagcga tatgggcatg
    12901 gaagccaccg aatacctgat gaaagacgtg cgcgaccgcg tcagcctcaa agggctgaaa
    12961 gacggcaacg aattgcgcgg cgcgctgaaa gaagccttgt acgacctgat taagcctcta
    13021 gagaaaccgc tggttttgcc cgaaaccaaa gagccgtttg tcatcatgct tgctggcatc
    13081 aacggcgcgg gcaaaaccac atccatcggc aaactcgcca aatatttcca agcgcagggc
    13141 aaatctgtgc tgcttgccgc cggcgatact ttccgcgccg ccgcgcgtga gcagcttcaa
    13201 gcttggggcg aacgcaacaa cgtaaccgtg atttcgcaaa ccacgggcga ttccgccgcc
    13261 gtgtgcttcg atgccgtcca agccgccaaa gcgcgcggca tcgacattgt gctggccgac
    13321 accgccggcc gcctgcccac gcagcttcac ttgatggaag aaatcaaaaa agtgaaacgc
    13381 gtgctgcaaa aagccatgcc cgacgcgcca cacgaaatca tcgtcgtgct tgatgccaat
    13441 atcggtcaaa acgccgtcaa ccaagtcaaa gcctttgacg acgcattggg gctgaccggt
    13501 ttaattgtta ccaagctaga cggcacggca aaaggcggca tcctcgccgc gcttgcctcc
    13561 gaccgccccg tccccgtccg ctatatcggc gtgggcgaag gcatagacga tttgcgcccg
    13621 tttgacgcgc gcgcgtttgt ggacgcactg ctggattgag ccgaaatgcc gtccgaaaac
    13681 agcagaccga tgccgtcatt cccgcgcagg cgggaatcta gaccttagaa taacagtaat
    13741 attcaaaggt tagctgaagc tttagagat
//
LOCUS       DAXHSF010000035        13678 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.035, whole genome shotgun sequence.
ACCESSION   DAXHSF010000035 DAXHSF010000000
VERSION     DAXHSF010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13678
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.035"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..1722
                     /locus_tag="ACFM8S_000806"
     CDS             <1..1722
                     /locus_tag="ACFM8S_000806"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - obsolete DNA methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC6313905.1"
                     /translation="LYTKLTRKRQEIFFNQTLAFDEIDRLFDAKAFSKFSRYTADGKQ
                     AVGEIKRHSDGTPAENLIIKGNNLIALHSLAKQFKGKVKLIYIDPPYYFVKKKQQDSF
                     GYNTNFKLSTWLTFMKNRLSIAKELLTDDGIIVISIDDDGNAYLKILLDEIFGFENFI
                     GNLPTIMNLKGNNDEYAFAGTHEYTLVFAKNKDKSTFYEFPIDEDNFLEKWEEDEIGF
                     YKKGAPMRATGTEDKREDRPEMFYPFLVKNNTVSTITDEEFSQIYNKDLEVFNDDFIQ
                     KLKEKYENLGYNFILPIADKQWGRWRWGYSIKNKARLQTDIIVSQSKNGISLYKKQRP
                     ELDDLPTKKPKTIFYKPEYSSGNGTEQMKNLFGEKAFKNPKPEELIQDFITITTNESD
                     IVLDYHLGSGTTAAVAHKMNRQYIGIEQMDYIETLAVERLKKVIDGEQGGISKAVNWQ
                     GGGEFVYAELAPFNETAKQQILACEDSDGIKTLFEGLCERYFLKYNVSVNEFSQIIQE
                     PEFQSLALDEQKQMVLEMLDLNQMYVSLSEMDDEQFAGCLNDDDKALSREFYQSAKNQ
                     AEKKDGE"
     gene            1712..4486
                     /locus_tag="ACFM8S_000807"
     CDS             1712..4486
                     /locus_tag="ACFM8S_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFC6313906.1"
                     /translation="MANNKTLFDWVEDRKSTLEEIEQTDFFALPEFVERNLKYPFFEW
                     QKSALENFVIFDRASKLKDFPDIKNRPTHLLFNMATGAGKTMMMAALILYYFEKGYRH
                     FLFFVNQNNIVDKTENNFIDPAHSKFLFTEKILQGDQVIPIHKVETFSPHSDGIEIKF
                     TSIQKLYNDIHTERENQTTLADLHELNLVMLGDEAHHLNAQTKGKKQGELDLEVELKA
                     NAGDAEFERKGWEHMVLELLLNKNGNPGQNVLLEFTATPPEKADVQQKYADKIITKFG
                     LKEFLQKGYTKEINLVSSTLSKKERVLHALLFAWYRHQIALKYGIANFKPVMLFRSKT
                     VDESKADYLAFLNWVENVQADDFSFLKTFSMSLNDSDNANEQGKTRTEQALKFMQENG
                     FEFAHLANWIKQNYQKHNIIITNSETNKSKTEKTDSETEKLLNNLEAVDNPIRAIFTV
                     GRLTEGWDVLNLFDIVRLYEGQNGGGSNKKSCKTANTTVSEKQLIGRGVRYFPFSFED
                     KQPNKRKFDNDMQHELRILEELFYYTHDEQSRYITELKNELRKDGYLPEKDDDKVLAT
                     FKLKSEFADNENFKNLLIWANKKIPNPNAKSNNADSLKANPQTLSFQIHGNQLLQETQ
                     FTADKNDEKVKQLGIQNNFSQTIKMSEMERHIFNKALHIKGKNSQSLFHFNRLQSKLN
                     IQNRNELQNNLLKDWQIEFLGLEQDKQVRPDDKLAGCLKILEMVEKHLNESDKPFIGT
                     KEFTPKTLWEIFGTPKQKWVKKDDIKTAIAMQNDWYVMDNFAGTGLEEALIQFISARL
                     GDLKSQYDIHLIRNEEVFKLNNFANGEGFMPDFILLLKDKQKSSSNGVNDFLHYQIFI
                     EPKGEHLVETDGWKEEFLEAITVEYGMDKILQKDTPHYRLIGLPFFTDHQKNGQFTES
                     FPLGVASLKK"
     gene            complement(4627..5799)
                     /locus_tag="ACFM8S_000808"
     CDS             complement(4627..5799)
                     /locus_tag="ACFM8S_000808"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFC6313907.1"
                     /translation="MKRDLSKMTCIEDLRRVAKRKMPRMFYDYIDSGSWTETTYRENT
                     SDFKDIRFRQKVLVNMEGRSLETKMIGQDVKMPVAIAPTGFTGMAHADGEILAARAAE
                     KFGIPFTLSTMSICSIEDVAENTSAPFWFQLYVMRDREFMENLIKRAKDAKCSALVLT
                     ADLQVLGQRHKDIKNGLSAPPKPTIANLINLATKPEWCMKMLNTERRTFRNIVGHAKN
                     VGDLSSLSSWTSEQFDPRLSWDDVARIKDLWGGKLIIKGIMEPEDAEKAAKSGADALI
                     VSNHGGRQLDDTVSAIKALPDIVSAVGSDIEVWMDSGIRSGQDILKAWALGAKGTMIG
                     RAFLYGLGAYGEEGVTRALEILYKEMDISMAFTGHRDIQDVDASILRGKDWGRETV"
     gene            6149..6595
                     /gene="iscR"
                     /locus_tag="ACFM8S_000809"
     CDS             6149..6595
                     /gene="iscR"
                     /locus_tag="ACFM8S_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFC6313908.1"
                     /translation="MRLTTKGRFAVTAMLDLAMNAQTGAVKLSAISERQNISLSYLEQ
                     LFGKLRRAGLVESLRGPGGGYILAAPAARINIAQIIAAAEDRLDATQCGSKANCHHGA
                     PCLTHDLWENLNKTINDYLGSVTLQSIIEQKNNGDGSRVVQFTHIH"
     gene            6624..7838
                     /locus_tag="ACFM8S_000810"
     CDS             6624..7838
                     /locus_tag="ACFM8S_000810"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HFC6313909.1"
                     /translation="MTVKTPVYLDYAATTTVDKRVAEKMIPYLTETFGNPASNSHAFG
                     WEAEEAVEKARADIAALINADPKEIVFTSGATESDNLAIKGAANFYKTKGKHLITVKT
                     EHKAVLDTMRELERQGFEVTYLGVQENGLVDLDVLKAAIREDTILVSVMWVNNEIGVV
                     QDIPAIGEICRERKIVFHVDAAQACGKVPVDVEAAKIDLLSMSAHKVYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGFRSGTLPTHQIVGMGEAFRIAKEELEQDMAHYRKLRDI
                     FLKGIEGIEEVYINGDLEHRAPNNLNVSFNFVEGESLIMAVKELAVSSGSACTSASLE
                     PSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKD
                     GIDLNSIEWAAH"
     gene            7984..8829
                     /locus_tag="ACFM8S_000811"
     CDS             7984..8829
                     /locus_tag="ACFM8S_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFC6313910.1"
                     /translation="MKIANALIHNTVRIECLSADGQSISTGTAFLFLFDFDKTGVIPV
                     LVTNKHVVFVESAKKIAITLTKDENGSPNHKENITFTIEDFIQNCLPHPDENIDLCIA
                     PVGHFFNQLINHNFSPFIKGIRESDIMQTEEMDQLSAFEEVFMIGYPNGLWDSKNNLP
                     IFRTGITATHPAIDFEGKKEFMIDVACFPGSSGSPVFKYIPAGLRKIENDGFAITADE
                     TFKLMGILYAGPQQTISGEIYITTVNKPISVSSIPINLGFVIKAEKLLDFKPILKNFL
                     EKNRE"
     gene            8976..9104
                     /locus_tag="ACFM8S_000812"
     CDS             8976..9104
                     /locus_tag="ACFM8S_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313911.1"
                     /translation="MNEQELDLDNLDNLLDDFDGVTVEGGVDSENDDGCEGGACKI"
     gene            9170..9556
                     /gene="iscU"
                     /locus_tag="ACFM8S_000813"
     CDS             9170..9556
                     /gene="iscU"
                     /locus_tag="ACFM8S_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFC6313912.1"
                     /translation="MAYSDKVIDHYENPRNVGTFDKNDESVGTGMVGAPACGDVMRLQ
                     IKVNDEGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLDDALAIKNSEIAEELELPPV
                     KIHCSILAEDAVKAAVADYRKRQENR"
     gene            9643..9963
                     /gene="iscA"
                     /locus_tag="ACFM8S_000814"
     CDS             9643..9963
                     /gene="iscA"
                     /locus_tag="ACFM8S_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFC6313913.1"
                     /translation="MITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFV
                     DEADGDDLIFEGHGARIYIDPKSLVYLDGTQVDYTKEGLQEGFKFENPNVKDSCGCGE
                     SFHV"
     gene            complement(9923..10144)
                     /locus_tag="ACFM8S_000815"
     CDS             complement(9923..10144)
                     /locus_tag="ACFM8S_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HFC6313914.1"
                     /translation="MGGKVQHNKGKIRDNALKALVKSDLFRHKVERKRKGKGSYNRQE
                     AKKWRDGFDTVPPFLCLKHGSFRRSRRSL"
     gene            10226..10726
                     /gene="hscB"
                     /locus_tag="ACFM8S_000816"
     CDS             10226..10726
                     /gene="hscB"
                     /locus_tag="ACFM8S_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001671 - ATPase activator activity
                     [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HFC6313915.1"
                     /translation="MSQYFTLFRIEPAFDIDTENLEQTYRALAARFHPDKFASASAFE
                     QKQAVMMSSTINDAYRTLKNPIDRAAYLLKTSGIDADAPEHTSFASEFLMQQMEWRET
                     LMEARAGKDLESLKNLDNEIRDEQEKLFCGLKQSFARQDYDTAAQQVRQGRFLDKLRN
                     EISSAL"
     gene            10824..13574
                     /gene="gyrA"
                     /locus_tag="ACFM8S_000817"
     CDS             10824..13574
                     /gene="gyrA"
                     /locus_tag="ACFM8S_000817"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFC6313916.1"
                     /translation="MTDATIRHDHKFALETLPVSLEDEMRKSYLDYAMSVIVGRALPD
                     VRDGLKPVHRRVLYAMHELKNNWNAAYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQ
                     NFAMRYVLIDGQGNFGSVDGLAAAAMRYTEIRMAKISHEMLADIEEETVNFGPNYDGS
                     EHEPLVLPTRFPTLLVNGSSGIAVGMATNIPPHNLSDTVNACLRLLDAPDTEIDELID
                     IIQAPDFPTGATIYGLSGVREGYKTGRGRVIMRGKTHIEPIGKNGEREAIVIDEIPYQ
                     VNKAKLVEKIGDLVREKTLEGISELRDESDKSGMRVVIELKRNENAEVVLNQLYKLTP
                     LQDSFGINMVVLVDGQPRLLNLKQILSEFLRHRREVVTRRTLFRLKKARHEGHIAEGK
                     AVALSNIDEIIKLIKESPNAAEAKDKLLARPWRSSLVEEMLTRSGLDLEMMRPEGLAA
                     NIGLKEQGYYLSEIQADAILRMSLRNLTGLDQEEIVESYKNLMGKIIDFVDILSKPER
                     ITQIIRDELEEIKTNYGDERRSEINPFGGDIADEDLIPQREMVVTLTHGGYIKTQPTT
                     DYQAQRRGGRGKQAAATKDEDFIETLFVANTHDYLMCFTNLGKCHWIKVYKLPEGGRN
                     SRGRPINNVIQLEEGEKVSAILAVREFPEDQYVFFATAQGMVKKVQLSAFKNVRAQGI
                     KAIALKEGDYLVGAAQTGGADDIMLFSNLGKAIRFNEYWEKSGNDEAEDADIETEISD
                     GIEDETADSENALPSGKHGVRPSGRGSGGLRGMRLPADGKIVSLITFAPETEESGLQV
                     LTATANGYGKRTPIADYSRKNKGGQGNIAINTGERNGDLVAATLVGETDDLMLITSGG
                     VLIRTKVEQIRETGRAAAGVKLINLDEGETLVSLERVAEDESELSDASVISNVTEPEV
                     EN"
BASE COUNT         4246 a         3287 c         2909 g         3236 t
ORIGIN      
        1 ttatacacta aattaacccg aaaaagacaa gaaatctttt ttaatcaaac gcttgctttt
       61 gatgaaatcg atcggctttt tgacgcaaaa gcattctcaa aattctctcg ctataccgca
      121 gacggcaaac aagcggttgg cgaaatcaaa cgtcattcag acggcacacc cgccgaaaat
      181 ctcattatca aaggcaataa tctgatagcc ctacattcgc ttgccaaaca gtttaaaggc
      241 aaagtgaagt tgatttatat tgatccgcct tattattttg ttaaaaagaa acaacaagat
      301 agttttggtt ataacactaa ctttaaatta tctacttggc ttacatttat gaaaaatcgt
      361 ttatcaattg ccaaagaatt attaactgat gatgggatta ttgttatttc cattgatgat
      421 gacggaaatg cttatttaaa gattttgtta gatgaaattt ttggatttga aaattttatc
      481 ggaaatttac ccactattat gaatttaaaa ggtaataatg atgaatatgc ttttgcaggc
      541 actcacgaat acacactagt ctttgctaaa aataaagata aatctacttt ttatgaattt
      601 ccaatagatg aagataattt tttagagaaa tgggaagaag atgaaattgg gttttataaa
      661 aaaggagctc caatgcgagc aacaggaacc gaagataaaa gagaagatag acctgaaatg
      721 ttctatcctt ttttggtaaa aaataacaca gttagtacaa ttacagatga agaattttct
      781 caaatctata ataaagattt agaagtattc aatgatgatt ttattcaaaa actaaaagag
      841 aaatacgaaa atcttggata caattttatt ttacctattg ctgataaaca atgggggcgt
      901 tggcgttggg ggtattcaat aaagaataag gcaaggctgc agacggatat tattgttagc
      961 caatctaaaa atggaatttc cttgtataaa aaacaaagac ctgaattaga tgatttacct
     1021 acaaaaaaac ctaaaactat tttttataaa cctgaataca gtagtggtaa tggaactgaa
     1081 cagatgaaaa atctttttgg tgaaaaggca tttaaaaatc ctaaaccaga agaacttatc
     1141 caagatttta ttaccatcac aaccaacgaa tccgacatcg tcttagacta ccatcttggt
     1201 agtggcacaa ccgccgccgt tgcccacaaa atgaaccgac aatatatcgg tattgaacaa
     1261 atggattata ttgaaacgct tgccgttgaa cgcttgaaaa aagtgattga tggcgagcaa
     1321 ggcggtattt ccaaagccgt gaattggcaa ggtggtggcg agtttgttta tgccgaactt
     1381 gccccattta acgaaaccgc aaaacaacaa attttggctt gcgaagattc agacggcatc
     1441 aaaacgctgt ttgaaggttt atgcgaacgc tatttcttga aatacaacgt cagcgtaaat
     1501 gaatttagtc aaatcattca agagcctgaa tttcaatcct tggcattaga cgaacaaaaa
     1561 caaatggtgc ttgaaatgtt ggatttaaat caaatgtatg tttcattatc cgaaatggat
     1621 gatgagcaat ttgcaggttg cttaaatgat gacgataaag cgttaagccg tgaattctat
     1681 caatcagcaa aaaatcaagc ggagaaaaaa gatggcgaat aataaaacgt tatttgattg
     1741 ggtggaagac cgcaaatcaa cgctggaaga aatagaacag acagattttt tcgcgctgcc
     1801 tgaatttgta gaacgcaatt taaaatatcc gttttttgaa tggcaaaaat cggctttaga
     1861 aaactttgtg atttttgacc gcgcttcaaa attaaaggat ttccctgaca tcaaaaaccg
     1921 ccctacccat ttgctgttca atatggcaac aggtgctggc aaaacgatga tgatggcggc
     1981 gttgattttg tattattttg aaaaaggtta tcggcatttt ttgtttttcg tcaatcaaaa
     2041 taatatcgtg gataaaacgg aaaataattt tattgacccg gcgcactcaa aatttttatt
     2101 taccgagaag attttgcaag gcgaccaagt tattcccatt cacaaagtag aaacgtttag
     2161 cccacattca gacggcattg aaattaaatt taccagtatt caaaaactgt ataacgatat
     2221 ccacaccgaa cgggaaaacc aaaccacatt ggcagatttg cacgaattga accttgtgat
     2281 gctgggcgat gaagcgcacc atttaaacgc acaaaccaaa ggcaaaaaac aaggcgagtt
     2341 ggatttggaa gtggaattaa aagccaatgc aggcgatgcc gaatttgaac gcaaaggctg
     2401 ggagcatatg gttttggaat tgttgctcaa taaaaatggc aatcccggcc aaaatgtgct
     2461 gttggaattt accgccacgc cacctgaaaa agctgacgtg caacaaaaat acgctgataa
     2521 aatcatcaca aaatttggtt taaaagaatt tttgcaaaaa ggttatacca aagaaatcaa
     2581 tttggtatcc agtacactga gcaagaaaga gcgggtgttg cacgctttat tgtttgcttg
     2641 gtatcgccat caaatcgctt tgaaatatgg cattgccaat ttcaagcctg tgatgttgtt
     2701 tagaagtaag acggttgatg aatcaaaagc ggattatttg gcatttttaa attgggtaga
     2761 aaatgtgcaa gcagatgatt tttcgttttt aaagacattt tcaatgagct taaacgatag
     2821 cgataacgcc aacgaacaag gcaaaacccg caccgaacaa gccctaaaat ttatgcagga
     2881 aaatggcttt gagtttgcac atttagcaaa ttggataaaa caaaattatc aaaaacacaa
     2941 tatcattatt accaactccg aaaccaacaa aagcaaaacc gaaaaaaccg acagcgaaac
     3001 ggaaaaattg ctaaacaatc tggaagcggt tgataatccg attcgtgcta tttttacggt
     3061 gggtagatta accgaaggtt gggacgtttt aaatttgttt gatattgtgc gtttgtatga
     3121 agggcaaaac ggcggcggtt caaataaaaa atcatgcaaa acggcaaata caacggtatc
     3181 agaaaaacag ttgattggtc gaggcgtgcg ttattttcca ttttcgtttg aagataaaca
     3241 gccgaataag cgtaaatttg acaacgacat gcaacacgaa ttgcgtattt tggaagaatt
     3301 gttttattac acgcacgatg agcaatcccg ctatattaca gaactgaaaa acgagttacg
     3361 aaaagacggt tatttgcctg aaaaagacga tgataaggta ttggcaacat ttaaactcaa
     3421 atctgaattt gcagataatg aaaattttaa aaacttattg atttgggcga ataaaaaaat
     3481 ccccaatccc aatgctaaaa gtaataatgc agacagccta aaagccaatc cgcaaacact
     3541 ttcattccaa attcacggca atcaactgtt gcaggaaacg caatttacag ccgataaaaa
     3601 tgatgaaaag gtcaagcaac ttggcataca aaataatttt tctcaaacca taaaaatgag
     3661 tgaaatggaa cggcacatct tcaataaagc attacatatc aaagggaaaa atagtcaatc
     3721 tttattccat ttcaaccgtt tgcaaagcaa actcaacatt caaaatcgca atgaattgca
     3781 aaataacttg ttgaaagact ggcaaattga atttttagga ttagagcaag ataaacaggt
     3841 tcgcccggat gataaacttg caggctgcct gaaaatcttg gaaatggttg aaaagcattt
     3901 gaatgaaagt gataagccat ttatcggtac aaaagaattt acgcctaaaa cattatggga
     3961 aattttcggc acgccaaaac aaaaatgggt caaaaaagat gatataaaaa ctgctattgc
     4021 catgcaaaat gattggtatg tgatggataa ttttgccgga acgggtttgg aagaagcgtt
     4081 aattcaattt atttcagcgc gtttgggcga tttgaaatct caatatgaca ttcatttaat
     4141 ccgtaacgaa gaagtgttta aattgaataa ctttgccaat ggcgaaggat ttatgcccga
     4201 ctttatttta ttgctgaaag ataaacaaaa atcttcttcc aatggcgtga atgacttttt
     4261 gcattaccaa attttcattg agcccaaagg tgagcattta gtggaaactg atggatggaa
     4321 agaagaattt ttagaagcca tcacagtgga atatgggatg gataagatcc tgcaaaaaga
     4381 tacaccgcat tatcgtttga tcggtttacc gttttttact gatcatcaga aaaatggaca
     4441 atttacggag tcattccctt taggtgtggc atcgcttaaa aaatagagta gtgcattgca
     4501 ggcagccccg tttgacaaaa cttcctttac aaaagggcgt tttgtcagat agttcattat
     4561 taaaatacag ccaaatttta atgccgtccg aaccatgtgt tcagacggca tcgtattttt
     4621 cagtatttaa accgtttccc taccccaatc tttacccctt aaaatcgagg catcgacatc
     4681 ttgaatatcg cggtgtcccg taaacgccat agatatatcc atttctttat acaggatttc
     4741 cagcgcacgg gttacgcctt cttcgccata cgcacccaag ccatacagga acgcccgacc
     4801 tatcattgta cctttcgcac ccaaagccca cgccttcaaa atatcctgac cgctgcggat
     4861 gccgctgtcc atccaaactt cgatgtcgct gccgactgca ctaacgatgt cgggcaaggc
     4921 tttgatggcg gacacggtat cgtcgagctg gcggccgccg tggttggaaa caatcaatgc
     4981 gtccgcgccg cttttcgctg ctttttccgc atcttcaggt tccataatgc ctttgataat
     5041 cagcttgcca ccccacaaat ctttaatgcg tgcaacgtcg tcccagctca ggcgcgggtc
     5101 gaattgttcg gaagtccatg aagacagcga ggacaaatcg ccgacgtttt tggcgtgtcc
     5161 aacgatattg cggaacgtgc ggcgttccgt gttcagcatt ttcatacacc attcgggctt
     5221 ggtcgccaga ttgattaaat tggcgatggt cggtttcggc ggcgcggaca ggccgttttt
     5281 gatgtctttg tggcgttgcc ccaaaacctg caaatcagcg gtcaatacca atgccgaaca
     5341 tttggcatcc ttcgcgcgct taatcaggtt ttccataaac tcgcggtcgc gcatcacata
     5401 aagctgaaac caaaatggtg cggaagtgtt ctcggcaacg tcttcaatcg agcagataga
     5461 catcgtggac aacgtaaacg gaatgccgaa cttctccgcc gcccgcgccg ccaagatttc
     5521 accgtctgcg tgcgccatac ccgtgaaacc agtcggggca atcgccaccg gcattttcac
     5581 atcctgccca atcattttgg tttcgaggct gcgcccctcc atattgacca atactttttg
     5641 tcggaaacga atgtctttga aatccgaagt attttcccga taagtcgttt ccgtccacga
     5701 acccgaatcg atgtaatcgt aaaacatacg cggcattttg cgcttggcaa cgcggcgcaa
     5761 gtcttcgatg caggtcattt tgctcaaatc acgtttcatc ttgtttttcc ctgtttgaca
     5821 gcctgatgga atatgccgtc cgaaaccgct tgagccttca gacagcattt caggcaatac
     5881 ccaaataaat cactaagatt tagataatct atatcaatat caaaaataaa tcaaatcgtt
     5941 ttgaaaccgc ttgccggcaa aataccccga ccgccgctcc tcaaaaaaca taaccaactg
     6001 atttaaaaaa ccaaacaaat aaagcaaaat cagactatac cgccatttaa acaaacaatc
     6061 ggcaacagct cccccttact tgactgaaac actcagatat tggacaattc tccccactat
     6121 aaaaaagccg acacgggcaa ccaccaccat gagactgacc accaaagggc gtttcgccgt
     6181 taccgctatg ctggatttgg cgatgaacgc gcaaaccggc gccgtcaaac tcagtgccat
     6241 cagcgaacgc caaaacatat ccctctccta tctcgagcaa ttgttcggca aactccgccg
     6301 cgccggactg gttgaaagcc tgcgcgggcc cggcggcggc tacatcctcg ccgcaccggc
     6361 ggcacgcatc aacatcgccc aaatcatcgc cgccgccgaa gaccggctgg acgcgaccca
     6421 atgcggcagc aaagccaact gccaccacgg tgcgccctgc ctgacgcacg acctttggga
     6481 gaatttaaac aaaaccatca acgactacct cggcagcgtt accctgcaaa gcatcatcga
     6541 acagaaaaac aacggcgacg gcagccgcgt cgtccaattt acacacatcc attaaataac
     6601 acccgaaaaa gaaagagcaa accatgaccg tcaaaacccc cgtttacctc gactacgccg
     6661 ccaccaccac cgttgacaaa cgcgttgccg aaaaaatgat tccctatctg accgaaacct
     6721 tcggcaaccc cgcctccaac agccacgcat tcggctggga agcagaagag gccgtcgaaa
     6781 aagcccgcgc cgacatcgct gccctgatta atgccgaccc caaagaaatc gtcttcacca
     6841 gcggcgcgac cgagtccgac aacctcgcca tcaaaggcgc ggcaaacttc tacaaaacca
     6901 aaggcaaaca cctcattacc gtcaaaaccg aacacaaagc cgttttagat acaatgcgcg
     6961 aactcgaacg ccaaggtttt gaagtaacct acctcggcgt tcaagaaaac ggtttggttg
     7021 atttagacgt actgaaagcc gccatccgcg aagacaccat cctcgtttcc gtaatgtggg
     7081 taaacaacga aatcggcgtg gttcaagata ttcctgccat cggcgaaatc tgccgcgaac
     7141 gtaaaatcgt cttccacgtc gatgccgccc aagcctgcgg caaagtgcct gtcgatgtcg
     7201 aagccgccaa aatcgacctg ctctcgatgt ccgcgcacaa agtgtacggc cccaaaggca
     7261 tcggcgcgct gtacgtccgc cgcaaacccc gcgtccgcct cgaagcccaa atgcacggcg
     7321 gcggccacga acgcggcttc cgcagcggca cattgccgac ccaccaaatc gtcggtatgg
     7381 gcgaagcctt ccgcatcgcc aaagaagaac tcgaacaaga tatggcgcac taccgcaaac
     7441 tgcgcgacat cttcctcaaa ggcatcgaag gcatcgaaga agtctacatc aacggcgacc
     7501 tcgaacaccg tgctccgaac aacctgaacg tcagcttcaa cttcgtcgaa ggcgaaagcc
     7561 tgattatggc agtcaaagaa ctcgccgtat ccagcggctc tgcctgtact tctgccagtc
     7621 tcgaaccaag ctacgtccta cgcgcgctcg gtcgcaacga cgaactggcg cactcatccc
     7681 tgcgcatcac cttcggtcgc atgaccaccg aagaagaagt gcaattcgcc gccgaactga
     7741 ttaaatccaa aatcggcaaa ctgcgcgaac tgtcgccgct gtgggaaatg ttcaaagacg
     7801 ggattgattt gaattcgatt gagtgggcgg cgcattaatt tatgaattag tacgatgata
     7861 tgagtatagt agataagatt ttcaaatatt ctcgccacct cgattattga gatatagtga
     7921 acttcttatc tacggataaa atttttatag atagtttaat atttaaatag aacttaaaaa
     7981 attatgaaaa tagccaatgc attaattcac aatacagtcc gtattgaatg cctttcagca
     8041 gacggccaat ctatatcaac gggaacggca ttcttattcc ttttcgattt cgacaaaaca
     8101 ggggtaattc cagttcttgt aacaaataaa catgttgttt ttgtagagtc cgcaaaaaaa
     8161 atagctatta ctttgacaaa agatgaaaat ggctctccaa atcataaaga aaatataact
     8221 tttacaattg aagattttat acaaaattgt ttacctcatc ctgatgaaaa tattgatcta
     8281 tgcattgctc ctgttggaca tttttttaat cagctgatta atcataattt ctcgcctttt
     8341 ataaaaggaa taagagaatc tgatattatg caaactgaag aaatggatca attatcagct
     8401 ttcgaagaag tttttatgat aggctatcct aatggcttat gggactcaaa aaacaactta
     8461 ccaatattta gaacaggaat tactgctacc catccagcta ttgattttga agggaaaaaa
     8521 gaatttatga ttgatgttgc ctgtttccct ggttcaagcg gttctccggt gttcaaatat
     8581 atacctgctg ggttaagaaa aatagaaaac gatgggtttg caattacagc agatgaaaca
     8641 tttaaattaa tgggaatcct atatgctgga cctcagcaaa caatatcagg ggaaatttat
     8701 ataacaactg ttaataaacc aatttctgta tcttctattc ctattaattt aggttttgtc
     8761 ataaaggcag aaaaactttt agactttaaa ccaattttaa agaatttttt ggaaaaaaat
     8821 agagaatgaa attatattaa aagattaata aatattagag actggcaatc caccttacct
     8881 aatctacatt aaaaataaaa aagaccgttt gaaacatcaa aaagattttt tcaaacgacc
     8941 taaaagccac cacatgaaat caaggataca aaaccatgaa cgaacaagaa cttgacctcg
     9001 acaacctcga caacctgctt gacgatttcg acggcgttac cgtggaaggc ggcgtcgatt
     9061 cggaaaacga cgacggctgt gaaggcggag cgtgcaaaat ctaactcagg ccgtctgaaa
     9121 accggcaaac aaaccactta aatcatcaaa acattaagga aaccacatca tggcatacag
     9181 cgataaagta atcgaccact acgaaaatcc ccgcaacgtc ggtactttcg acaaaaacga
     9241 cgaatctgtc ggcaccggca tggtcggcgc accggcctgc ggcgacgtga tgcgcctgca
     9301 aatcaaagtg aacgatgaag gcatcatcga agatgcgaaa ttcaaaactt acggctgcgg
     9361 ttccgccatc gcttcgtcca gcctgattac cgagtgggtt aaaggcaaaa gcctggatga
     9421 cgcgctggca atcaaaaaca gcgaaatcgc cgaggagttg gaattgccgc cggtaaaaat
     9481 ccactgctcc atcttggctg aagatgcggt aaaagcggcc gttgccgact accgcaaacg
     9541 tcaggaaaac agataaagcc cttcagacgg catcgtcccg caatgccgtc cgaaccaccc
     9601 gccgcttcag gtccgtccgg ggcggtacaa caaggaagaa atatgattac ccttaccgag
     9661 aatgccgcaa aacacatcaa tgactatctc gccaaacgcg gcaaaggctt gggcgtacgc
     9721 ttgggtgtga aaaccagcgg ctgctcgggg atggcgtaca accttgaatt tgtcgacgaa
     9781 gccgatggcg acgacctgat tttcgaagga cacggcgcgc gcatttatat cgatccgaaa
     9841 agcctggttt atctggatgg cacgcaagtc gattacacca aagaaggttt gcaggaaggt
     9901 ttcaaatttg aaaaccccaa tgtcaaagac tcctgcggct gcggcgaaag cttccatgtt
     9961 taagacataa aaatggcggg accgtatcaa aaccgtcccg ccattttttc gcttcctgcc
    10021 tgttgtagct gcctttgcct ttccttttcc gttccacctt gtgccggaac aaatcggatt
    10081 tcactaaggc ttttaaagca ttgtcgcgta ttttgccttt attgtgctgc actttgccgc
    10141 ccatattcag tcctttcgtt taagaagcgg cagattataa ggcaaaaaca gttttctgcc
    10201 aaaatcttac atttatcatc ctactatgtc ccaatatttc accctcttcc ggattgaacc
    10261 cgctttcgat atcgacaccg aaaacttgga acaaacctac cgcgccttgg ccgcccgttt
    10321 ccatcccgat aaattcgctt cagcttccgc ctttgagcaa aagcaggcag tgatgatgtc
    10381 ttccaccatc aacgatgcct accgcacctt gaaaaaccct atcgaccgcg ccgcctacct
    10441 gctgaaaaca tcgggcatcg atgccgacgc gccggagcat acctctttcg cctccgaatt
    10501 ccttatgcag caaatggaat ggcgcgaaac gctgatggag gcacgggcgg gcaaagacct
    10561 tgaatccttg aaaaatctcg acaacgaaat ccgcgacgaa caagaaaaac tgttctgcgg
    10621 tctgaaacag tcgtttgccc gacaagatta cgacaccgcc gcacaacaag tccgccaagg
    10681 caggtttctc gacaaactcc gcaacgaaat ttcctcggca ttataatccg caccgtgttt
    10741 cagacggcgt aaccgccgca ccgttccgcg tcaaaatatg ctaaaataag caacaatttt
    10801 ttgccatacg aaacattgaa accatgaccg acgcaaccat ccgccacgac cacaaattcg
    10861 ccctcgaaac cctgccggta agccttgaag acgaaatgcg caaaagctat ctcgactacg
    10921 ccatgagcgt cattgtcggg cgcgcgctgc cggacgttcg cgacggtctc aagccggtac
    10981 accgccgcgt actgtacgcg atgcacgaat tgaaaaacaa ctggaatgcc gcctacaaaa
    11041 aatcggcgcg catcgtcggc gacgtcatcg gtaaatacca cccccacggc gataccgccg
    11101 tatacgacac catcgtccgt atggcgcaaa atttcgctat gcgttatgtg ctgatagacg
    11161 gacagggcaa cttcggatcg gtggacgggc ttgccgccgc agccatgcgc tacaccgaaa
    11221 tccgcatggc gaaaatttcc cacgaaatgc tggcagacat tgaggaagaa accgtcaatt
    11281 tcggcccgaa ctacgacggt agcgaacacg agccgcttgt actgccgacc cgtttcccca
    11341 cactgctcgt caacggctcg tccggcatcg ccgtcggcat ggcgaccaat atcccgccgc
    11401 acaacctttc cgataccgtc aatgcctgcc tgcgcctgct cgatgcaccc gacaccgaaa
    11461 tcgacgaact gatcgacatt atccaagccc ccgacttccc gaccggggca accatctacg
    11521 gcttgagcgg cgtgcgcgaa ggctataaaa caggccgcgg ccgcgtcatt atgcgcggta
    11581 agacccatat cgaacccata ggcaaaaacg gcgaacgcga agccatcgtt atcgacgaaa
    11641 tcccctatca ggtcaacaaa gccaagctgg tcgagaaaat cggcgatttg gttcgggaaa
    11701 aaacactgga aggcatttcc gagctccgcg acgaatccga caaatccggt atgcgcgtcg
    11761 ttatcgagct gaaacgcaac gaaaatgccg aagtcgtctt aaaccaactc tacaaactga
    11821 ctccgctgca agacagtttc ggcatcaata tggtggtttt ggtcgacgga caaccgcgcc
    11881 tgttgaacct gaaacagatt ctctccgaat tcctgcgcca ccgccgcgaa gtcgttaccc
    11941 gacgtacgct tttccggctg aagaaggcac gccatgaagg gcatatcgcc gaaggcaaag
    12001 cggtcgcact gtccaatatc gatgaaatca tcaagctcat caaagaatcg cccaacgcag
    12061 ccgaggccaa agacaaactg cttgcgcgtc cttggcgcag cagcctcgtt gaagaaatgc
    12121 tgacgcgttc cggtctggat ttggaaatga tgcgtccgga aggattggct gcaaacatcg
    12181 gcttgaaaga gcaaggttat tacctgagcg agattcaggc agatgctatt ttacgcatga
    12241 gcctgcgaaa cctgaccggc ctcgatcaag aagaaattgt cgaaagctac aaaaacctga
    12301 tgggtaaaat catcgacttt gtggatatcc tctccaaacc cgaacgcatt acccaaatca
    12361 tccgcgacga actggaagaa atcaaaacca actatggcga cgaacgccgc agcgaaatca
    12421 acccgttcgg tggcgacatt gccgatgaag acctgattcc gcaacgcgaa atggtcgtta
    12481 ccctgacaca tggcggctat atcaaaaccc agccgaccac cgactatcag gcgcagcgtc
    12541 gcggcgggcg cggcaaacag gcggctgcca ccaaagacga agactttatc gaaaccctgt
    12601 ttgttgccaa cacgcatgat tatttgatgt gctttaccaa tttgggcaag tgtcattgga
    12661 ttaaggttta caaactgccc gaaggcggac gcaacagccg cggccgtccg attaacaacg
    12721 tcatccagtt ggaagaaggc gaaaaagtca gcgcgattct ggcagtacgc gagttccccg
    12781 aagaccaata cgtcttcttc gccaccgcgc agggaatggt gaaaaaagtc caactttccg
    12841 cctttaaaaa cgtccgcgcc caaggcatta aagccatcgc gctcaaagaa ggcgactacc
    12901 tcgtcggcgc tgcgcaaaca ggcggtgcgg acgacatcat gctgttctcc aacttaggta
    12961 aagccatccg cttcaacgaa tactgggaaa aatccggcaa cgacgaagcg gaagatgccg
    13021 acatcgaaac cgaaatttca gacggcatcg aagatgaaac cgccgacagc gaaaacgcac
    13081 tgccgagcgg caaacacggt gttcgcccgt ccggtcgcgg cagcggcggt ttgcgcggta
    13141 tgcgcctgcc tgccgacggc aaaatcgtca gcctgattac cttcgcccct gaaaccgaag
    13201 aaagcggttt gcaagtttta accgccaccg ccaacggata cggaaaacgc accccgattg
    13261 ccgattacag ccgcaaaaac aaaggcgggc aaggcaatat tgccattaac accggcgagc
    13321 gaaacggcga tttggtcgcc gcaaccttgg tcggcgaaac cgacgatttg atgctgatta
    13381 ccagcggcgg cgtacttatc cgcaccaaag tcgaacaaat ccgcgaaacc ggccgcgccg
    13441 cagcaggcgt gaaactgatt aacttggacg aaggcgaaac cttggtatcg ctggaacgtg
    13501 ttgccgaaga cgaatccgaa ctctccgacg cttctgtaat ttccaatgta accgaaccgg
    13561 aagtcgagaa ctgaaaatca tctcccgatg ccgtctgaag attcagacgg catttatttt
    13621 atccctcatc cgtcatccag cttctcacaa tatagcggat tatagtcaat taaaaaca
//
LOCUS       DAXHSF010000036        13638 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.036, whole genome shotgun sequence.
ACCESSION   DAXHSF010000036 DAXHSF010000000
VERSION     DAXHSF010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13638
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.036"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(31..1557)
                     /locus_tag="ACFM8S_000818"
     CDS             complement(31..1557)
                     /locus_tag="ACFM8S_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HFC6313917.1"
                     /translation="MKTRSLISLLCLLLCSCSSWLPPLEERTESRHFNTSKPVRLDNI
                     LQIRHTPHTNGLSDIYLLNDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNL
                     MYLAAERGVRVRLLLDDNNTRGLDDLLLALDSHPNIEVRLFNPFVLRKWRALGYLTDF
                     PRLNRRMHNKSFTADNRATILGGRNIGDEYFKVGEDTVFADLDILATGSVVGEVSHDF
                     DRYWASHSAHNATRIIRSGNIGKGLQALGYNDETSRHALLRYRETVEQSPLYQKIQAG
                     RIDWQSVQTRLISDDPAKGLDRDRRKPPIAGRLQDALKQPEKSVYLVSPYFVPTKSGT
                     DALAKLVQDGIDVTVLTNSLQATDVAAVHSGYVKYRKPLLKAGIKLYELQPNHAVPAT
                     KDKGLTGSSVTSLHAKTFIVDGKRIFIGSFNLDPRSARLNTEMGVVIESPKIAEQMER
                     TLADTTPEYAYRVTLDRHNRLQWHDPATRKTYPNEPEAKLWKRIAAKILSLLPIESLL
                     "
     gene            complement(1669..3075)
                     /locus_tag="ACFM8S_000819"
     CDS             complement(1669..3075)
                     /locus_tag="ACFM8S_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC6313918.1"
                     /translation="MEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNTALPEIAADM
                     NESPLDMQLAVVAYTLTVALLIPLSGYLADRFGTKKVFFGSIAVFMLGSALCAASGSL
                     FELTLSRVVQGIGGSMLVPIPRLTILRVYDKSKLLNAINYAVMPALIGPVLGPLAGGY
                     LVEYASWHWIFLLNLPIGLLGFILGRNIMPDVKGNDTALDFKGYLIFSAAACLLLLSA
                     ESLSHALPPYFALLPLCGGLLFARRYFRHMKTASKPIYSADLFLIRTFRLGLAGNLFS
                     RLGISSIPFLMPLMFQVAFGFGASLSGWLVAPVALSSLLVKPLIAPLMKRFGYRTVLL
                     WNTKLLAAFIMLLALPDGNSPLWIWIFLSLAIGACNSLQFSAMNTLTLADLRPQQTGS
                     GNSLMAVNQQLAISMGIVVGALILKNWTFLIPASSGLHSAFRMTLLSIGGITLASSLV
                     FKRLHVSDGANLTRNTLS"
     gene            3359..4138
                     /locus_tag="ACFM8S_000820"
     CDS             3359..4138
                     /locus_tag="ACFM8S_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFC6313919.1"
                     /translation="MSATIPPKIIRYDSNPTDVYFFGTCVLDLFMPEAGMDAIALIEQ
                     QGIRVHFPMAQSCCGQPAYSSGHPTEAFDVAKAQLDLFPENWPIVVPSGSCGGMMKHH
                     WPTLFKGSEYEERAVDCAGRIIEFTHFLLAIGFKPEDKGEPLKVAVHTSCAARREMNV
                     HLSGWQLIDGMENVERIVHDHESECCGFGGTFSVKQADISGAMVTDKVAALKETGATE
                     IISADCGCMMNIGGKIAKDEPDMPRPKHIASFLLERTGGKA"
     gene            4135..4836
                     /locus_tag="ACFM8S_000821"
     CDS             4135..4836
                     /locus_tag="ACFM8S_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFC6313920.1"
                     /translation="MSARENILAKLKKADALPMEEPAVFDYYREKGVSWDSEAERLKH
                     WAAAMRAVKTEIYWVTKSNWMQVFREAAEGKGLKNILLPLATEHGQIARAALAGSNIE
                     PIVFEREIDTWKTEFFTNINAGFSGAQCGIARTGTLMLFSSPEEPRTLSLVPPVHFCL
                     FDTSKMYNEFHNAVEGEKLVENGMPTNVFLISGPSKTADIQLTLAYGAHGPRDLVILA
                     ILPDHISPADLEENA"
     gene            4833..6287
                     /locus_tag="ACFM8S_000822"
     CDS             4833..6287
                     /locus_tag="ACFM8S_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFC6313921.1"
                     /translation="MTTQTIKFHMKPETFKQNAAISLQDKPLRKSLRTAMDMLMTKRK
                     AVLTDEEELQSLRDLCEHIRQRSLSKLPALLEQLEENLTKLGVKVHWAETPAEACEII
                     HDIITAKNGKLMVKGKSMVSEEIELNHYLEAKGIKAVESDLGEFIVQMAGEKPTHIVM
                     PAIHKTKEQVSELFHQNLGTPLTDDVDQLTGFARKALRDIYSTADVGLSGVNFAVAET
                     GTLCLVENEGNGRLSTTVPPVHIAITGIEKVVAKLSDIPPLYSLLPRSAIGQNITTYF
                     NMITGPRRSEELDGPQEMHLVLLDNGRSQAYAEDQMRRTLQCIRCGACMNHCPVYTRI
                     GGAAYGTTYPGPIGEIISPHLLGLDATRDLPTACTMCGACVEVCPVRIPITEQMQRLR
                     VEAQRSPTETVPHPIRGQGASHTFGEQMAWRTFNGIFSGSKTYRAFGWAATKFRNLTP
                     RKQLGWTQNRVPMKPAKKTLHELMAEKMRQKEQA"
     gene            complement(6470..6955)
                     /gene="purE"
                     /locus_tag="ACFM8S_000823"
     CDS             complement(6470..6955)
                     /gene="purE"
                     /locus_tag="ACFM8S_000823"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFC6313922.1"
                     /translation="MIQIGIIMGSNSDWPVMRQAAQFLEEFGVEYEACVVSAHRTPDL
                     MFQYAETARARGIKAIIAGAGGAAHLPGMVAAKTTVPVLGVPVPSKYLRGEDSLLSIV
                     QMPKGVPVATFAIGEAGAANAALFAVSMLANENPELAQKLADFRAKQEQAVLSMELEQ
                     I"
     gene            complement(7003..7719)
                     /locus_tag="ACFM8S_000824"
     CDS             complement(7003..7719)
                     /locus_tag="ACFM8S_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system YbgF family protein"
                     /protein_id="HFC6313923.1"
                     /translation="MKTKLPLFIIWLSVSAACSSPVSRNIQDMRPEPQAEAGSSDAIP
                     YPVPTLQDRLDYLEGTLVRLSNEVETLNGKVKALEHAKTHPSSRAYVQKLDDRKLKEH
                     YLNTEGGSASAHTVETAQNLYNQALKHYKSGRFSAAAALLKGADGGDGGSIAQRSMYL
                     LLQSRARMGNCESVIEIGGRYANRFKDSPTAPEAIFKIGECQYRLQQKDIARATWRSL
                     IQAYPSSPAAKRAAAAVRKR"
     gene            complement(7719..8387)
                     /locus_tag="ACFM8S_000825"
     CDS             complement(7719..8387)
                     /locus_tag="ACFM8S_000825"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6313924.1"
                     /translation="MTTHLSNVAPDLQNYLNAIGEPEHPVLTRLREKTGHHRMGKMAI
                     AREQAAVLVWLAKLIRVEKYLEVGVFTGYSSTALALALPEHGRITACDINVTFTDTAR
                     QVWNEAGVAHKISLHLQPALLTLDDLIAQGEAGSYDLALIDADKPPTPQYFERCLKLV
                     RQGGIIAIDNILLNGRVMREAASDAPPSVGILKDFNQNLPNDTRIVPITLPVGDGLTL
                     LLKK"
     gene            complement(8398..8583)
                     /locus_tag="ACFM8S_000826"
     CDS             complement(8398..8583)
                     /locus_tag="ACFM8S_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HFC6313925.1"
                     /translation="MYHYQSEATQFLNRLIEEKPELEQQRLENRGLLWDVELNPEEQE
                     NFEAAKVAKKPYTYYQD"
     gene            8726..10702
                     /gene="mutL"
                     /locus_tag="ACFM8S_000827"
     CDS             8726..10702
                     /gene="mutL"
                     /locus_tag="ACFM8S_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFC6313926.1"
                     /translation="MPRIAALPDHLVNQIAAGEVVERPANALKEIVENSIDAGATAID
                     VELAGGGIRLIRVSDNGGGIHPDDIKLALHRHATSKIKTLNDLEHVASMGFRGEGLAS
                     IASVSRLTLTSRQDGSAHATQVKAEDGKLSSPTAAAHPVGTTIEAAELFFNTPARRKF
                     LKSEATEYAHCATMLERLALAHPHIAFSLKRDGKQVFKLPAQSLHERIAAIVGDDFQT
                     ASLEIDSGNGALRLYGAIAKPTFAKGKTDKQYCFVNHRFVRDKVMLHAVKQAYRDVLH
                     NALTPAFVLFLDLPPEAVDVNVHPTKTEIRFRDSQQVHQLVFHTLNKALADTRANLTE
                     SVGNAGEVLHDITGVVSTPMPSENDSENLFDSVSNYPTGNKSDTHNAFGSSGKTAPMP
                     YQSAYAPQQRSLSLRESRAAMNTYAELYKKTDDIDLELSRFEQARFGNMPSETPAPQT
                     DTPLSDGIPSQSELPPLGFAIAQLLGIYILAQAEDSLLLIDMHAAAERVNYEKMKRQR
                     QENGRLQSQRLLIPVTFAASHEECAALADHADALAGFGLELSDMGGNTLAVRTVPAML
                     GKADVVSLAKDVLGELAQVGSSQTIEEHENRILATMSCHGSIRAGRRLTLPEMNALLR
                     DMENTPRSNQCNHGRPTWVKLTLKELDALFLRGQ"
     gene            10797..12926
                     /gene="dnaX"
                     /locus_tag="ACFM8S_000828"
     CDS             10797..12926
                     /gene="dnaX"
                     /locus_tag="ACFM8S_000828"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFC6313927.1"
                     /translation="MAYQVLARKWRPKTFSDLVGQEHVVKALQNALDEGRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCENAQHGEPCGVCQSCTQIDAGRYVDLLEIDAASNTGID
                     NIREVLENAQYAPTAGKYKVYIIDEVHMLSKSAFNAMLKTLEEPPEHVKFILATTDPH
                     KVPVTVLSRCLQFVLRNMTAQQVADHLAHVLDSEKITYAPPALQLLGRAAAGSMRDAL
                     SLLDQAIALGSGKVAENDVRQMIGAVDKQYLYELLTGIINQDGAALTAKAQEMAACAV
                     GFDNALGELAILLQHLALIQAVPSALAHDDPDSDILHRLAQTISGEQIQLYYQIAVHG
                     KRDLSLAPDEYAGFMMTLLRMLAFAPLAAASCDANAVIENTELKSPSAQTAEKETAAK
                     KPQPRPEAETAQTPVQTASAAAMPSESKTAEPVSNQENNDVPPWEDAPDEAQTAAGTA
                     QTSAKSIQTASEAETPPENQVSKNKAADNETNAPLSETPSENPIQATPNNEAVETETF
                     AHEAPAEPFYGYGFPDNDCPVEDGAEIPPPDWEHAAPADTAGGGADEEAEAGGIGGNN
                     TPSAPPPEFSTENWAAIVRHFARKLGAAQMPAQHSAWTEYHPDTGLMVLAMTAEARAT
                     ADKKRLDKIRDTLAQAYGLQLTLQTQDWRDEAGRETPAMQDKRVQAEDRQKAQALLEA
                     DPAAQKILQAFGAQWQPESVELAANRP"
     misc_feature    11986..12025
                     /gene="dnaX"
                     /locus_tag="ACFM8S_000828"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01843"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="This ribosomal frameshift element is known only in
                     the genus Neisseria; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01843"
     gene            13006..13341
                     /locus_tag="ACFM8S_000829"
     CDS             13006..13341
                     /locus_tag="ACFM8S_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFC6313928.1"
                     /translation="MFGKAGLGGLMKQAQQMQENMKKAQAKLAETEIEGEAGNGLVKI
                     TMTCAHEVRKIDISPDLIQEAADDKEMLEDLILAALKSARGKAEETANKTMGAFTQGL
                     PPGVGDFFR"
BASE COUNT         3363 a         3857 c         3678 g         2740 t
ORIGIN      
        1 cgtactggtt tttgttaatc cgctatattt ctataataaa ctttctatgg gcagcaggga
       61 taggattttt gcggcgatgc gtttccaaag tttggcttcg ggttcgttcg ggtaggtttt
      121 tcgggtggcg ggatcatgcc attgcaggcg gttgtgcctg tcgagggtaa cgcggtaggc
      181 gtattcgggt gtggtatcgg caagggtgcg ctccatctgt tctgcgattt tggggctttc
      241 gataacaacg cccatttcgg tgttgagacg cgcggaacgg gggtcgaggt tgaacgaacc
      301 gatgaagatg cgtttgccgt ccacaatgaa ggttttggcg tgcaggctgg ttacggagct
      361 gccggtcagg cctttgtctt ttgtggcggg gacggcatgg ttgggttgca gctcgtagag
      421 tttgatgccg gctttgagca gcggttttcg gtatttgaca tagccggaat ggacggcggc
      481 aacgtcggtc gcctgtagcg agttggtcag aacggtaacg tctatgccgt cctgcaccag
      541 ttttgccagt gcgtctgtgc cggattttgt ggggacgaaa tagggtgaaa ccagatagac
      601 gcttttttcg ggctgtttga gcgcgtcttg cagccgcccg gcaatcggcg gtttacggcg
      661 gtcgcggtcg agtccttttg cagggtcgtc gctgatgagg cgggtttgga cgctctgcca
      721 gtcgatgcgt cctgcctgta ttttttggta gaggggcgac tgttcgacgg tttcgcggta
      781 gcgcaggagc gcgtgtctgg acgtttcgtc gttgtatccg agtgcttgaa gacccttgcc
      841 gatgttgccg ctgcggatga tgcgcgtggc gttgtgggcg gaatggcttg cccagtagcg
      901 gtcgaagtcg tgcgatactt cgccgacgac gctgccggtg gcgaggatgt ccaggtcggc
      961 gaaaacggtg tcctcaccga ctttgaagta ttcgtcgccg atattgcgtc cgccgagtat
     1021 ggtggcgcgg ttgtcggcgg taaaggattt gttgtgcatg cggcggttga ggcgggggaa
     1081 gtcggtcagg tagccgagtg cgcgccattt tcgtaagacg aaggggttga acaggcgcac
     1141 ttcgatattg ggatggctgt cgagggcgag caggaggtcg tccaatccgc gcgtgttgtt
     1201 gtcgtccaac agcaggcgta cgcgcacgcc gcgttctgcg gcaaggtaca tcaggttgaa
     1261 cagcagtcgg ccggaaatgt cgttgcgcca gatgtagtat tgcaaatcga ggctgtgttc
     1321 ggcagattcg ataagggcgg cgcgggcggc aaaggcttcg tgggggtcgt tcaacagata
     1381 gatatcggat agcccgttgg tatgaggggt gtgccggatt tgcaggatgt tgtccaggcg
     1441 gacgggtttg gaagtattga aatgacggct ttccgtccgt tcttccagtg ggggcaacca
     1501 tgaagaacat gaacagagaa ggaggcataa aagggaaatt aggctgcgtg ttttcatcag
     1561 ggatatggtt tcagacggca ttgcctgtgt tttggggttg gcgcgcatgg atgtgcggaa
     1621 tcataatcca aacgttgaaa cgggtaaaag ttttgcgtgc ggaccgcttt aggacagtgt
     1681 gttccgtgtc aggtttgcgc cgtctgaaac gtgcagacgc ttgaaaacca acgacgaggc
     1741 aagggtgatg ccgccgatgc tgagcagggt catacggaag gcggaatgca gacctgaaga
     1801 agccggtatc agaaatgtcc agtttttaag gattaatgcg ccgacaacaa tgcccatgct
     1861 gatggcaagc tgttggttga ccgccatcag gctgttgccg ctgccggtct gttgcgggcg
     1921 caaatcggcg agggtcagtg tgttcatggc agaaaactgt agggagttgc acgcgccgat
     1981 cgccagcgag aggaaaatcc aaatccacag cggcgagttt ccatcaggca gggcgagcag
     2041 catgatgaag gcggcaagca gcttggtgtt ccaaagcagt accgtgcggt agccgaaacg
     2101 tttcatgagc ggtgcaatca gcggtttgac cagcagcgaa gacagggcga cgggggcgac
     2161 cagccaaccc gacaggcttg cgccgaagcc gaaagcgact tgaaacatca ggggcatcag
     2221 aaaaggaatc gagctgatgc cgagacggct gaacagattg cccgccagtc ccagacggaa
     2281 agtgcgtatc agaaacaggt cggcggaata aatcggtttg gacgcggttt tcatatgtcg
     2341 gaaataacgg cgtgcaaaca gcagtccgcc gcacagcggc aacagtgcaa aatacggagg
     2401 cagcgcgtgc gacaggcttt ctgccgaaag taacaagagg cacgcggcgg cagaaaaaat
     2461 cagatagcct ttgaaatcca gagcggtatc gttgcctttg acatcgggca tgatgttgcg
     2521 tcccaatatg aaacccaaaa gaccgatggg caggttgagc aggaaaatcc agtgccacga
     2581 agcgtattcg accaaataac cgcccgccaa aggccctaaa accggcccga ttaatgcggg
     2641 cataaccgca taattgatgg cattgagcag cttggacttg tcgtacacac gcaagatggt
     2701 cagacgcggt atcggaacca gcatcgaacc gccgatgccc tgaacgacac gggaaagcgt
     2761 caattcaaac agcgaacccg atgcggcgca caatgccgat ccgagcataa aaacggcaat
     2821 cgaaccgaaa aagacttttt tcgttccgaa cctgtccgcc aaataaccgc tcaaaggaat
     2881 cagcagggca accgtcagcg tataggcaac gactgccagt tgcatatcca gaggcgactc
     2941 attcatgtcg gcggcaattt caggcagtgc ggtatttaaa atggtcgcat ccaacatctg
     3001 cataaaaatg gcaattgcca gcagaagcgg cagccaaggg gatggtgcgc gggcggatag
     3061 ggtgtttttt tccatagggc gattgtaccc catccttgtg ccgttattgt tttcagatgc
     3121 tgtctgaatg ccgtcagagt cggcgtcctg aatgttcaca agcaaacgaa tcagcatttt
     3181 attgtaatga taattattat cgaaaattat cagagtaagg tacagtaagc gttatggggc
     3241 agtttgtaaa aaaaaccgga ttatttttta aaattagact tgacccgcaa cagtcaatta
     3301 cttaaagtaa acgcttacct ttctacagag aaaaacgggt ttcccgttat caaaaaacat
     3361 gagcgcaacc attcccccaa aaatcatccg atacgacagc aatccgaccg atgtctattt
     3421 tttcggcact tgcgtccttg atctttttat gcccgaagca ggcatggatg ccattgccct
     3481 aatcgagcag cagggcatac gcgtccattt cccgatggcg caaagctgct gcggccagcc
     3541 tgcctattca tccggccatc cgaccgaagc cttcgatgtc gccaaagcgc aacttgacct
     3601 tttccccgaa aactggccga ttgtcgtacc gtccggttcg tgcggcggta tgatgaaaca
     3661 ccattggccg acgctgttta aaggcagcga gtacgaggaa agggctgtgg attgcgccgg
     3721 ccgcatcatc gagtttaccc atttcctgct tgccatcggt ttcaaacccg aagacaaggg
     3781 cgaaccgctc aaagtcgccg ttcacacttc ctgcgccgcc cgccgcgaaa tgaatgtcca
     3841 tctttcaggc tggcaactga ttgacggtat ggaaaacgtc gaacgcatcg tccacgacca
     3901 cgaaagcgaa tgttgcggct tcggcggcac attctccgtc aaacaagccg atatttccgg
     3961 cgcgatggta accgacaaag tcgccgcgct gaaagaaacc ggcgcaaccg aaatcatcag
     4021 cgcggactgc ggctgtatga tgaacatcgg cggcaaaatc gccaaggacg agccggatat
     4081 gccgcgtccg aaacatatcg catccttctt gttggaacgc accggaggca aagcatgagc
     4141 gcgcgtgaaa atattttggc aaaactgaaa aaagccgatg ccttgccgat ggaagagcct
     4201 gcggttttcg attattaccg cgaaaagggc gtttcttggg acagcgaagc cgagcgtctg
     4261 aaacattggg ctgccgcaat gcgcgcggtc aaaaccgaaa tttattgggt gacgaaaagc
     4321 aattggatgc aggttttccg cgaagcggca gaaggcaagg gtttgaaaaa catcctgctg
     4381 cccttggcga ccgaacacgg acaaattgcc cgtgccgcat tggcgggcag caatatcgaa
     4441 ccgattgtct tcgagcgcga aatcgatact tggaaaaccg agtttttcac gaacatcaat
     4501 gcgggcttca gcggcgcgca atgcggcatc gcccgcaccg gcacgctgat gctgttttcc
     4561 agccccgaag aaccgcgtac tttgagtctc gttccgcccg tgcatttctg cctgttcgat
     4621 acgtccaaga tgtacaacga gtttcataat gccgtcgaag gcgaaaaact ggtggaaaac
     4681 ggtatgccga ccaatgtatt cctgatttcc ggcccgtcca aaaccgcaga catccaactg
     4741 acgcttgctt acggcgcgca cggcccgcgc gatttggtca tcctcgccat cctgcccgac
     4801 cacatttccc ctgccgattt ggaggaaaac gcatgactac gcaaaccatc aaatttcaca
     4861 tgaagccgga aactttcaag caaaacgccg caatttccct tcaagacaag cctttgcgca
     4921 aaagcctgcg taccgcgatg gatatgctga tgaccaaacg caaagccgtt ttgaccgacg
     4981 aagaagagct gcaaagcttg cgcgatttgt gcgaacacat ccgccagcgt tccctgtcca
     5041 aattgcccgc gctgttggag cagttggaag aaaacctgac caagttgggc gtaaaagtac
     5101 actgggcgga aacaccggcc gaagcctgcg aaatcatcca cgacatcatc acagccaaaa
     5161 acggcaagct gatggtcaaa ggcaaatcga tggtcagcga ggaaatcgag ctgaaccatt
     5221 atcttgaagc aaaaggcatt aaagcggtag aaagcgactt gggcgagttc atcgtccaaa
     5281 tggcaggcga aaaaccgacc catatcgtga tgcctgcaat ccataaaacc aaagaacagg
     5341 tgagcgaact gttccaccaa aacctcggca caccgcttac agacgatgtg gaccaactga
     5401 ccggcttcgc ccgcaaagcc ctgcgcgata tttacagcac tgccgatgtc ggcttgagcg
     5461 gcgtgaactt cgccgttgcc gaaacaggta cgctgtgtct ggtggaaaac gaaggcaacg
     5521 gtcgcttgag taccaccgta ccgcccgtgc atatcgctat taccggcatt gaaaaagtgg
     5581 tggcgaaatt gtccgacatc ccacccttgt acagtctgct gccgcgctcc gccatcggtc
     5641 aaaacatcac aacctatttc aatatgatta ccggcccgcg ccgcagtgaa gaattagacg
     5701 gtccgcaaga aatgcacttg gttctgctcg acaacggccg cagccaggct tatgccgaag
     5761 accaaatgcg ccgcaccctg caatgtatcc gttgcggcgc gtgtatgaac cattgcccgg
     5821 tttatacccg catcggcggc gcggcatacg gcacaaccta tcccggcccg attggcgaga
     5881 ttatttcccc tcacctgttg ggtctggatg ccactcgcga cctgccaacc gcctgtacca
     5941 tgtgcggtgc gtgtgtggaa gtctgtccgg tacgcatccc gattaccgaa caaatgcagc
     6001 gtttgcgcgt tgaagcgcaa cgttcgccga ccgaaaccgt gccgcacccc atccgagggc
     6061 aaggcgcatc gcataccttc ggcgaacaaa tggcgtggcg cacattcaac ggtattttca
     6121 gcggcagcaa aacctaccgc gccttcggtt gggcagccac caagttccgc aacctgaccc
     6181 cgcgcaaaca gttgggttgg acgcaaaacc gcgtgccgat gaaaccggcg aagaaaacct
     6241 tgcacgaact gatggcggaa aaaatgcgcc aaaaagaaca ggcataaaaa gttgttcgca
     6301 aaaatgccgt ctgaaacccg aaacagggct tcagacggca tttgtatagt ggattaacaa
     6361 aaatcaggac aaggcgacga agccgcagac agtacaaata gtacggcaag gcgaggtaac
     6421 gccgtactgg tttaaattta agccactata tattcgcgga cggcgggttt taaatctgtt
     6481 ccaattccat actcaaaaca gcctgttcct gtttggctcg gaagtctgcc agtttttgcg
     6541 ccagttcggg gttttcgttg gccagcatgg aaacggcgaa caatgcggca tttgccgcgc
     6601 ctgcctcgcc gatggcgaat gtggcgacgg gtacgccttt gggcatttgt acaatcgata
     6661 aaagcgaatc ttcgccgcgc aggtatttgc tggggacggg tacgcccaaa acggggacgg
     6721 tggtcttggc ggcaaccata ccgggtaaat gcgccgcgcc gcccgcaccc gcgatgatgg
     6781 ctttgatgcc gcgcgcccgt gcggtttcgg cgtattggaa cattaaatcc ggggtgcggt
     6841 gtgcggaaac gacgcacgcc tcatattcta cgccgaactc ttcaagaaac tgcgctgcct
     6901 gccgcataac gggccaatcg ctgttgctgc ccatgatgat gccgatttga atcataaatc
     6961 ctccttggtg cggatggggt aaaaagcgga aaaatggaaa aactatcgtt tgcgcacggc
     7021 tgcggcggcg cgttttgccg ccgggctgct cgggtaagcc tgtatcaggc tgcgccaagt
     7081 cgcccttgca atgtctttct gctgcaacct gtattggcat tcgccgattt tgaatatggc
     7141 ttcgggcgcg gttgggctgt ctttgaaacg gttggcgtaa cgccctccga tttcgatgac
     7201 ggattcgcag ttgcccatac gcgccctgct ttgcagcaac aggtacatac tgcgttgcgc
     7261 aatgctgccg ccgtcgcctc cgtccgcgcc tttcaacagg gcggctgcgg cagaaaacct
     7321 gccgctttta tagtgtttga gtgcctgatt gtagaggttt tgtgcggttt cgacggtatg
     7381 tgcggatgcg ctgccgcctt cggtattgag gtaatgctct ttcaacttgc ggtcgtcgag
     7441 tttttggacg tatgccctgc tggaagggtg tgttttcgca tgctccagtg ctttgacttt
     7501 gccgtttaag gtttccactt cgttcgacag gcggacgagt gtgccttcca gataatccaa
     7561 acggtcttgc agagtgggaa cgggataggg aatagcgtcc gaactacctg cctctgcctg
     7621 cggttcgggc cgcatatcct gaatattgcg ggaaacaggg gaagaacagg cggcggatac
     7681 ggacagccaa atgataaaaa gcggtaattt ggtcttcatt attttttcag aagcagggtc
     7741 aaaccgtcgc cgacgggcag ggtgatgggg acaatccgcg tatcgttcgg caggttttga
     7801 ttaaaatctt tgaggatgcc gacgctgggc ggcgcatcgg aagccgcttc gcgcatcacc
     7861 cttccgttca acaaaatatt gtcgatggcg atgatgccgc cttgccggac aagcttgagg
     7921 caacgctcga aatattgcgg cgtgggcggt ttgtctgcgt ctatcagtgc caaatcgtag
     7981 cttccggctt caccctgtgc aatcaaatca tccaatgtca gcaatgcggg ttgcaggtgc
     8041 aggctgattt tatgtgccac acctgcctcg ttccaaacct gacgcgccgt atcggtaaag
     8101 gttacattga tgtcgcaggc ggtaatccgc ccgtgttcgg gcaacgccaa tgcaagcgcg
     8161 gtgctgctgt atccggtaaa cacgccgact tccagatatt tttccacgcg aatcagcttt
     8221 gccagccaaa ccaaaaccgc cgcctgttcg cgcgcaatcg ccattttgcc catacggtga
     8281 tgcccggtct tctcgcgcag ccgcgtcaaa acgggatgtt cgggttcgcc gatggcgttc
     8341 aaatagtttt gcaggtccgg tgcgacattg gacagatggg tcgtcatttc ggcggattca
     8401 gtcttggtaa taggtataag gttttttcgc cacttttgcc gcctcgaagt tttcctgttc
     8461 ttcgggattg agttcgacat cccacaaaag cccccggttt tccaaacgct gctgttccaa
     8521 ctcaggtttt tcttcaatca ggcggttgag gaattgtgtg gcttcggatt ggtagtgata
     8581 catctttgcg ctccaatttt acgggatacg gcgtgattat actggtattt tccaaacggg
     8641 ataaacggct tttatcaaga atacaggcag aaaagtaagg ggttttatta tagaataaag
     8701 cgtttttgca acggaagcct accttatgcc acgcattgcc gccctgcccg accatctcgt
     8761 caaccaaatc gccgccggcg aagtggtcga acgccctgcc aacgccttga aagaaatcgt
     8821 tgaaaacagt atcgatgcag gcgcaacggc gattgatgtc gagctggcag gcggcggcat
     8881 ccgcctgatt cgcgtcagcg acaacggcgg cggtatccat ccggacgaca tcaaactcgc
     8941 actccaccgc cacgctacca gcaaaatcaa aaccttaaac gatttggaac acgtcgccag
     9001 tatgggcttt cgcggcgaag gcttggcaag catcgcctcc gtcagccgcc tgaccctgac
     9061 cagccgccaa gacggcagcg cacacgcgac acaagtcaaa gccgaagacg gcaaactcag
     9121 cagccctacg gcagccgccc accccgtcgg cacgaccatc gaagccgccg aactcttctt
     9181 caatacccct gcgcggcgca agttcctcaa gtctgaagcc accgaatacg cccactgtgc
     9241 cacgatgctc gaacgcctcg cgctggcgca tccgcacatt gccttctcgc tcaaacgcga
     9301 cggcaaacaa gtgttcaaac ttcctgccca aagcctgcat gaacgtattg ccgccattgt
     9361 cggcgatgat tttcagacgg catccttaga aatagacagc ggcaacggcg cgctgcggct
     9421 ctatggcgcg attgctaagc cgactttcgc caaaggcaaa accgacaaac aatactgctt
     9481 cgtcaaccac cgcttcgtgc gcgacaaagt catgctccat gccgtcaagc aggcataccg
     9541 agacgtattg cacaacgcgc tgacgcctgc tttcgtcctc ttcctcgacc tgccgcccga
     9601 agccgtggat gtcaacgtcc acccgaccaa aaccgaaatc cgcttccgcg acagtcagca
     9661 ggtgcaccaa cttgtgttcc acacgctcaa caaagccctt gccgacacac gcgccaacct
     9721 gaccgaaagc gtcggcaacg caggcgaagt gttgcatgac attaccggcg ttgtctccac
     9781 cccaatgccg tctgaaaacg acagcgaaaa tctgtttgat agcgtatcca actacccgac
     9841 aggcaacaaa tcagatacac acaatgcctt tggttcatca ggcaaaaccg cgcccatgcc
     9901 ctatcagtcc gcatatgcgc cgcaacaacg cagcctgtcc ctgcgcgaaa gccgcgcggc
     9961 aatgaatact tacgccgaac tttacaaaaa aaccgacgac atcgaccttg agttaagccg
    10021 attcgagcag gcacgtttcg gcaatatgcc gtctgaaacg cctgctcccc aaacagatac
    10081 gccgctttca gacggcatcc cgtcccaatc cgaactgccg ccgctcggtt ttgccattgc
    10141 ccaactatta ggcatctaca ttcttgccca agccgaagac agcctgctgc tcatcgatat
    10201 gcacgccgcc gccgaacgcg tcaactacga aaaaatgaaa cgccaacgtc aggaaaacgg
    10261 caggctgcaa agccaacgcc tgcttattcc cgtaaccttt gccgcgtccc acgaagaatg
    10321 cgccgccctt gccgaccatg ctgatgcact ggcaggcttc ggcttggaac tgtccgacat
    10381 gggcggcaat acccttgccg tccgcacagt tcccgccatg ctcggcaaag ccgacgtcgt
    10441 ctcactcgcc aaagacgtat tgggcgaact cgcccaagtc ggcagcagcc aaaccatcga
    10501 agaacacgaa aaccgcatcc tcgccactat gtcctgccac ggctcgatcc gcgccggccg
    10561 ccggcttacc ctgcccgaaa tgaacgccct tctgcgcgat atggaaaaca cgccgcgcag
    10621 caaccagtgc aaccacggca ggccaacttg ggtcaaactg actttgaaag aattggacgc
    10681 actgttcttg cgcggacagt aagccgaaag tgctagaata cgccgcccga agccgccgtt
    10741 cagacggcat tccgacgcac cgacagaaac atcacgaccg aaaccaagag aaaaacatgg
    10801 cctatcaagt tctcgcccga aaatggcggc ccaaaacctt ttccgactta gtcggtcagg
    10861 aacacgtcgt caaagccctg caaaatgcct tggacgaagg caggctgcac cacgcctacc
    10921 tgctgaccgg tacgcgcggc gtgggcaaaa ccaccatcgc ccgcatcctt gccaaaagcc
    10981 tcaactgcga aaacgcgcaa cacggcgaac cttgcggcgt gtgccaaagc tgcacccaaa
    11041 tcgatgccgg acgctatgta gatttattgg aaatcgatgc cgcttccaac acaggcatcg
    11101 acaacatccg cgaagtattg gaaaacgccc aatacgcgcc gaccgccggc aaatacaaag
    11161 tctatatcat cgacgaagtg catatgcttt ccaaaagcgc gttcaacgct atgctcaaaa
    11221 cgctggaaga gccacccgaa cacgtcaaat tcatccttgc cacgaccgat ccgcacaaag
    11281 tccccgttac cgtcttgagc cgctgcctgc aatttgtctt acgcaatatg accgcgcagc
    11341 aggttgccga ccacctcgcc cacgtcctcg acagcgaaaa aatcacctac gccccccccg
    11401 ccctgcaact tttgggacgc gccgccgccg gatcgatgcg cgatgccttg agcctgctcg
    11461 accaagccat cgccctgggt tcgggcaaag ttgccgaaaa cgatgtccgc caaatgatcg
    11521 gcgcggttga caaacaatac ctttacgaac tgctgacagg catcatcaac caagacggcg
    11581 cagccctgac cgccaaagcg caggaaatgg cggcgtgtgc cgtcggcttt gacaacgcct
    11641 tgggcgaact tgccatactg ctgcaacacc tcgccctgat acaggcagtc ccctccgcct
    11701 tggcgcacga cgaccccgat tccgatattt tgcaccgcct cgcccaaacc ataagcggcg
    11761 aacaaatcca gctttactac caaatcgccg tccacggcaa acgcgacctg agcctcgccc
    11821 ccgacgaata cgccggcttt atgatgaccc tgctgcgtat gctggcgttt gcgcccttgg
    11881 cagcagcatc gtgtgatgca aatgccgtga ttgaaaatac cgaactaaaa tccccatcgg
    11941 cacaaaccgc cgaaaaggaa accgccgcaa aaaagcccca accgcgccct gaagcggaaa
    12001 ccgcccaaac acccgttcag acggcatccg cagcggcaat gccgtctgaa agcaaaactg
    12061 ccgaacccgt ttccaatcaa gaaaacaacg atgttccgcc ttgggaagac gcgccggacg
    12121 aagcccaaac cgcagccggc acggcgcaaa cgtcggcaaa aagcattcag acggcatccg
    12181 aagccgaaac gccgcccgaa aaccaagttt ccaagaacaa ggcagccgac aacgaaacca
    12241 atgccccctt gtccgaaacg ccgtctgaaa accccattca ggcaacaccg aataatgaag
    12301 ccgttgaaac ggaaacattt gcacacgaag cccctgcaga acctttctac ggttacggct
    12361 ttccggataa tgactgcccc gtagaagacg gcgcagaaat cccgccgccc gattgggaac
    12421 acgccgcccc tgccgatacc gcaggcggag gcgcggacga agaagcagaa gcaggcggca
    12481 tcggcggaaa caacacgccg tccgccccgc cgcccgaatt ttccaccgaa aactgggcag
    12541 ccatcgtccg gcatttcgcc cgcaaactcg gcgcggcgca aatgccggcg caacactccg
    12601 cgtggacgga ataccatccc gacaccggcc tgatggtttt ggcgatgacc gccgaagccc
    12661 gcgccaccgc cgacaaaaaa cgtctcgaca aaatccgcga cacccttgcc caagcctacg
    12721 gactgcaact caccctgcaa acccaagact ggcgcgacga agccggcagg gaaacccccg
    12781 cgatgcagga caagcgcgtc caagccgaag acaggcaaaa agcacaagca ttgctcgaag
    12841 ccgaccccgc cgcacaaaaa atcctccaag cattcggcgc gcaatggcag cccgaatcag
    12901 tggaattggc agcaaaccgg ccataaacag atataatgcc gcccgaaccc ttcggacggc
    12961 attgccgttt ccctcattca atcaaaacag acaggagtat tcagtatgtt cggaaaagcc
    13021 ggattaggcg gcctgatgaa acaggcgcag caaatgcagg aaaatatgaa aaaagcgcaa
    13081 gccaaacttg ccgaaaccga aatcgaaggc gaagcaggca acggcctggt caaaatcaca
    13141 atgacctgcg cgcacgaagt acgcaaaatc gacatcagcc ccgatttgat tcaagaagcc
    13201 gccgacgaca aagaaatgct cgaagacctc atcctcgccg ccctcaaatc cgcccggggc
    13261 aaagccgaag aaaccgcaaa caaaacaatg ggcgcattca cgcaaggtct accccccgga
    13321 gtgggcgact tcttccgctg atacccgacc gtcattccca cgcaggcggg aatctagaac
    13381 gtagaatcta agaaaccgtt ttactcgata aatttccgtg ccgacgggtc tggattcccg
    13441 ccttcgcggg aatgacggca tcagtttgca ggattcggcg tgaacggtaa aaacagtgag
    13501 aatgataaga acgcaaaaac ggcaagaata gcgggaatcg gcaggctgaa gcccacccta
    13561 ccattattta cacatccgta ccgcttaaat gccgtctgaa acttcgtcat tcccgtgaaa
    13621 gcgggaatcc aaccccgt
//
LOCUS       DAXHSF010000037        13613 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.037, whole genome shotgun sequence.
ACCESSION   DAXHSF010000037 DAXHSF010000000
VERSION     DAXHSF010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13613
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.037"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(307..417)
                     /locus_tag="ACFM8S_000830"
                     /pseudo
     CDS             complement(307..417)
                     /locus_tag="ACFM8S_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            437..1063
                     /locus_tag="ACFM8S_000831"
                     /pseudo
     CDS             437..1063
                     /locus_tag="ACFM8S_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751227.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(1052..1399)
                     /locus_tag="ACFM8S_000832"
                     /pseudo
     CDS             complement(1052..1399)
                     /locus_tag="ACFM8S_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255955.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
     gene            1710..2462
                     /locus_tag="ACFM8S_000833"
     CDS             1710..2462
                     /locus_tag="ACFM8S_000833"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFC6313929.1"
                     /translation="MITLPSLNNLPSKWDEIRYWLETQVFECEVMPYPNLTEHEAKQF
                     ERDFWDNIGCAPEEYVRIRRAIRLLEARYPDSLNELVCAAIATPLGEMLAVFGNKGLC
                     LLEFVGQKHLEQEIAAVQKALRGRFVFREDERTQLLRQELDLYFQCRLKTFATPLEMI
                     GTAFQKQAWDALLAIPYGETRSYKEQAQHLGNPKAVRAVAAANGQNKVSVLIPCHRVI
                     GSDGKLTGYAGGLNRKQFLLALERGEVQTALF"
     gene            2653..2728
                     /locus_tag="ACFM8S_000834"
     tRNA            2653..2728
                     /locus_tag="ACFM8S_000834"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2686..2688,aa:Asn,seq:gtt)
     gene            2859..3869
                     /gene="waaF"
                     /locus_tag="ACFM8S_000835"
     CDS             2859..3869
                     /gene="waaF"
                     /locus_tag="ACFM8S_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HFC6313930.1"
                     /translation="MSIKILIISPSWIGDCVMTQPLFRRLKELHPGCTIDVFAPKWSM
                     AVFERMPEVNEILENPFGHGALELKRRWRVGRDLGRRGYDQVIVLPGSLKSAVIALAT
                     GIAKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHPSQEDFDGHSGFPEFSID
                     ERRREISVETFGLNLGKPVLAFCPGAEFGPAKRWPTRHFAELGKHYLAAGWQVWLFGS
                     QKDDEIAEEINCLSDGMCVNLCGKTDLSQAMDLLSLADTVVCNDSGLMHLAAALGRKV
                     VAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEE
                     AV"
     gene            3924..4370
                     /gene="smpB"
                     /locus_tag="ACFM8S_000836"
     CDS             3924..4370
                     /gene="smpB"
                     /locus_tag="ACFM8S_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFC6313931.1"
                     /translation="MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESY
                     IYWKKDAFYLVGCHITALPTASTHIKPDAVRPRKLLLNQSEINKLIGKTERAGYTIVP
                     LDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR"
     gene            4744..6933
                     /locus_tag="ACFM8S_000837"
     CDS             4744..6933
                     /locus_tag="ACFM8S_000837"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFC6313932.1"
                     /translation="MKLEASKQKFKKSFIISLFFSILYTSPLLAVDYVYDKTKLTDDE
                     ITRLKKLRDRNSEYWKDDLFRIDIPKETGLRHDIEGAAKGNFSYPIFYIPTDDKPIDP
                     DKKISFFDSPYTPGYTAAFVQGFGVKERNGYTEEQAKKYIDELRTKLKTAPIIAAAQL
                     GYFQLNDGSGDSWLYNYYRNDIFNSYGEIKHSNINSEILAVGNVADLYYKHFIRNPFI
                     YVDWSIFSLPLPRLKVSENSHVIGQIIHLYRLDLEDSLWEPRWDSDVSYLNLFNSHIR
                     FNTKNDSLVVGESRIRPTPDNALNTEKYLKSQFSDTGYHSSVRSTYIGYPSIRFAYDL
                     SEREADKPVLTLKSKVTGKTAIVFEEKALNNLKNLTYRQLIKTETDVEPDAFFLLEEY
                     KKGRYRLFLRQCPNGFCIGVEKLAIPTHLVASYAQQAQAANTLFNLRLNDKNSDIFDR
                     TLPRKGLWLRVIDGHSNQWVQGKTAPVEGYRKGVQLGGEVFTWQNESNQLSVGLMSGQ
                     AEQRSTFRNPDTDNLTTGNVKGFGAGVYATWHQLQDKQTGAYADSWVQYQRFRHRINT
                     EDATERFTSKGITASIEAGYNALLAEHFTKKGNSLRVYLQPQAQLTYLGVNGKFSDSE
                     NAHVNLLGSRQLQTRVGVQAKAQFSLYKNIAIEPFAAVNALYHNKPFGVEIDGERRVI
                     NNKTAIESQLGVAVKIKSHLTLQATFNRQTGKHHQAKQGALNLQWTF"
     gene            complement(7114..8481)
                     /locus_tag="ACFM8S_000838"
     CDS             complement(7114..8481)
                     /locus_tag="ACFM8S_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFC6313933.1"
                     /translation="MPDLHTEFSRLLPADEIAELSPTLLKDQRNRFTSAPDIILQPRS
                     VESVQTIMRFCYEHRIPVTPQGGNTGLCGAAVSENGVLLNLSKLNRIRSINLSDNCIT
                     VEAGSVLQTVQQAAEASNRLFPLSLASEGSCQIGGNIACNAGGLNVLRYGTMRDLVIG
                     LEVVLPNGELVSHLHPLHKNTTGYDLRHLFIGSEGTLGIITAATLKLFARPSDKATAW
                     VGIPDIESAVRLLTETQAHFAERLCSFELIGRFAAELSSEFSKLPLPTHSEWHILLEL
                     TDSLPDSNLDDRLVEFLYKKGFTDSVLAQSEQERIHMWALRENISASQRKLGTSIKHD
                     IAIPIGRVADFVRRCAKDLEQNFKGIQIVCFGHLGDGSLHYNTFLPEILSNEVYRYEN
                     DINDAVYRNVLACNGTIAAEHGIGIIKKQWLDKVRTPAEIALMKSIKQHLDPYNIMNP
                     GKLLP"
     gene            complement(8591..8887)
                     /locus_tag="ACFM8S_000839"
     CDS             complement(8591..8887)
                     /locus_tag="ACFM8S_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313934.1"
                     /translation="MKKSLFAAALLSLVLAACGGEKAAEAPATEAPAAEAPATEAPAA
                     EAPAAEAPAAEAPATEAPAAEAPAAEAAATEAPAAEAAASEAPAAEAPAAEAAK"
     gene            9360..9842
                     /locus_tag="ACFM8S_000840"
     CDS             9360..9842
                     /locus_tag="ACFM8S_000840"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC6313935.1"
                     /translation="MAIVKNSVVSLHYEMYDANNQLLDKTEEPIAYLHGGYDGIFPLV
                     EEALHGKDAGDTVDVALSPDDAFGEQDPELVRIEDAGVFPVEVEVGMMFEADDLETGD
                     VVVYRVTDVADGKAVVDGNHPLAGMKIRFKATVESVRDASDEEIAHGHVHGPHGHHHH
                     "
     gene            9929..10741
                     /gene="hpnC"
                     /locus_tag="ACFM8S_000841"
     CDS             9929..10741
                     /gene="hpnC"
                     /locus_tag="ACFM8S_000841"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HFC6313936.1"
                     /translation="MSVGHYENFPVGSLILPRRLRKPVHAVYAFARTADDMADEGSMP
                     SEARLAGLEGLRRELDVLASGGRSAHPLIARLDAEAVVPFGLDLQPFYDLLSAFSQDV
                     VKTRYAHFGELADYCRRSANPVGRIMLALYGKTDAVCVAQSDGICTALQLVNFWQDVA
                     VDWQKGRVYIPQDDLLKFGVSEGQIAEGRADAAFQRLMAYECRRAFRMLKAGSPLARE
                     LKGRIGLELRMIVLGAQLILQKLDACRYDVFAQRPVLDKKDWLIMLKRALWK"
     gene            complement(10812..11282)
                     /locus_tag="ACFM8S_000842"
     CDS             complement(10812..11282)
                     /locus_tag="ACFM8S_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HFC6313937.1"
                     /translation="MYDVNTHDVRRFFARVWQQRLNPLQLSALEQKALRIIEAHPEYH
                     RYLERIEDHLDTDWLPENGESNPFLHMSLHLSVQEQAGIDQPHGIRAIHDTLCAKRGW
                     LEAEHEMMEALAETLWTAQRYGTGLDVNFYMTRLRKLIGLGAEDQARLNPHEIA"
     gene            11373..13214
                     /locus_tag="ACFM8S_000843"
     CDS             11373..13214
                     /locus_tag="ACFM8S_000843"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFC6313938.1"
                     /translation="MQTNYLSETDSIMKKLICLFAVFLMLCGRAFALDANDLLPPEKA
                     FVPELAVADDGVNVRFRIADGYYMYQAKIVGKTDPADLLGQPSFSKGEEKEDEFFGRQ
                     TVYHHEAQVAFPYAKAVGEPYKLVLTYQGCAEAGVCYPPVDTEFDISGNGTYHPQTDE
                     PASAKDRFLQPSSQNGSGALPPPKGDEGGDSRFKLSWDTLNANLLAFFLAGLGLSFTA
                     CMYPLLPIVSSIVVGDKKAGKARAFVLSVVYVQGLALTYTLVGIVAGLTGALLTVWLQ
                     QAWVVLAASALMVVLALSMFGLFNIQLPNAVQSYFQNQSSRLSGGKIVSVFIMGILSA
                     LIVGPCVAPPLAFALGYIGQTGDAVLGGLALYTLALGTGVPLIAIGTFGGHILPRAGD
                     WMNAVKYAFGFILLAVAVYLATPHLPYYLVVALYTLLMLVPACMLLVNGRRQKRRPKA
                     VAFALGGILLIGGAWFGWQGANGKTTALHHFLTLNPPAEAGKSSEHGKMFADTAALKA
                     AMDTALKEHPDKPVVLDFYADWCISCKEMAAYTLNQPEVHQAVDMERFFQIDVTANTP
                     EHQALLKEYGLFGPPGVFVVRSDGSRSEPLLGFVKADKFIEWYEQNR"
BASE COUNT         3316 a         2988 c         3783 g         3526 t
ORIGIN      
        1 acattgattt ttatgtagat ttttttattt aaacggcaat ccattccctc tcccgtggga
       61 gagggttagg gagagggcat cggggcggta acggaaatat tgtctgtatc tgccccgttt
      121 taccctctcc ccaaccctcc cccacagggg agggagtcaa gttgcaggca cgccgacgga
      181 atttggcttc gcattaactg ccttattttg aacgcacgtc aaacttaaat ttgcaatgcc
      241 gtctgaaaag aagatgggct gtcggataag gatgtccgac catttgctta gacctaacgt
      301 gggtttgaga tatagtggat taaatttaaa ccagtacggc gttgccttgc cttgccgtac
      361 gatttgtact gtctgcggct tcgtcgcctt gtcctgattt aaatttaatc cactatactt
      421 tcatacgcgc gaacgattgg gtgctttggg cggcatcgtg caacatcata ccgccttgta
      481 ccgcagcgtg gcggtatatg aaccagctgt gcttatcgtg cggcgcggaa gcaagaagct
      541 gcggtgggcg aggcgcgaat tgaggattgc ggcgggtgag gcggttgcgt tggcaggcgg
      601 acagacgttt gacgttatta atattcccga ttcagacggc ctctatcagg cgcaatggat
      661 tgctttcgaa caggaagcca tagaaaggtt tgccgcacaa tacgggacgg cgcaggcagt
      721 gtgcgatgcg gtgaagctgc cgcaccccgg gcggatgggc gcggcgtttg attatgcggc
      781 ggcggtgctg acggatgaag aagtgccgca caatgcggcg gaggtcgcgc tttgcggcgt
      841 gttggcgtgg ttgcagcatg acggcatcgg ttttgccgtg tacgaaggcg tcaacctgat
      901 gcggcaaatc cgcaaactga ttaccgccga tatggctgcg gactggtcgt cggcgatgtt
      961 ggcgcagcgg cttaattgca gcgaggccgc gttgcggcgg cggacatcgg cgggaatgtc
     1021 ggcgaccacc cagtgcatcc agcccagtcc ggtcggcgat gttggcgcag cggcttaatt
     1081 gcagcgaggt cgcgttgcgg cggtggacat cggcgggaat gtcggcgacc acccggtgca
     1141 tccagcccag tccggtcggc gcgtctttat cgtaaacggt caggacgaaa ctttttgtcc
     1201 cggcgggcgg atttttccac gacagcgcgg gcgaagcatt gccgcccgaa cagccaaatc
     1261 cgtaaggcgc gctcaaaagc tggttttgcg tgaagccgcc gtcttcggac gggttgtcga
     1321 attgcagcgt gaatgcgccc ccggcaaagg cagcggttga aaaagccagc agtacggcgg
     1381 agagaatgcg ggtttgcata gatgattcct ttgcttgatg aataagtgga ttcattatag
     1441 gctgtcaacg gcggcaggaa tgtgccgaaa cggttggatg atgtgccaga atgcgcaaat
     1501 gcaaaaatcg tggtttcaga cgagcctttg caggtcgtct gaagtcttta attcctttcc
     1561 ctgaggcttt ggaaatatcg catatttagg cattgccgca acctaaccct caagatttac
     1621 gtcgtagccc gttcctgcat cagcgtgcgg gaatggcgaa aattgggaat tttgccgtca
     1681 aatccatgat agagatacgg agcaaacgaa tgattaccct gccttcctta aacaatctgc
     1741 cgtcaaaatg ggatgaaatc cgctattggc tcgaaaccca agtttttgag tgcgaagtta
     1801 tgccgtatcc caatctgacg gaacacgaag ccaagcagtt cgagcgggat ttttgggaca
     1861 atatcggctg cgcgcctgaa gaatatgtcc gcatccgccg cgccatccgt ttgttggaag
     1921 cgcgttatcc cgacagcttg aacgagctgg tgtgtgccgc cattgccacg cctttgggcg
     1981 agatgctggc ggtgttcgga aataagggat tgtgtctgct ggagtttgtc gggcagaagc
     2041 atttggaaca ggaaattgcc gccgtccaaa aagccttgcg cggacggttt gtgtttcggg
     2101 aggatgagcg gacgcaactt ttgcggcagg aattggattt gtatttccaa tgccgtctga
     2161 aaacctttgc cacgcctttg gaaatgattg gtacggcgtt tcaaaaacag gcgtgggacg
     2221 cgcttttggc gattccttac ggtgaaacgc gcagctacaa ggaacaggcg cagcatttgg
     2281 gcaaccccaa agccgtccgc gccgttgccg ccgccaacgg gcagaacaag gtgtctgtcc
     2341 tgattccctg ccaccgcgtc attggcagcg acggcaaact gaccggctac gcaggcggtt
     2401 tgaaccgcaa acagttttta ctggctttgg aacgcggcga agttcagacg gcattgtttt
     2461 aagcggggga gggcagaacg gctcatcggg cttaaatatg gtgtgatgac ggctgtggaa
     2521 attcatacaa cggcaatcct tttaaggcat cggtcttaat gtctttctga ttcatatgct
     2581 tgtcgttttg tcgcccattt ttccaaaatt aaggattgac acattttttc cggacgctat
     2641 aatacgcccc attccccgat agctcagtcg gtagagcgac ggactgttaa tccgcaggtc
     2701 cctggttcga gcccaggtcg gggagccaga attttgaagc ccgaacttga atgttcgggc
     2761 tttttcatat ttgcacgtac ggcgttcgga atgagacaag ataggttttc caacggtttc
     2821 gcggtaaaat tctgcctgtc tgatttcggt ttcttgttat gtccatcaaa atcctgatta
     2881 tttctcccag ttggataggc gactgcgtga tgacccagcc cttgttccgc cgtttgaagg
     2941 aacttcaccc cggttgcacg attgatgtgt tcgcgccgaa gtggtcgatg gcggtgttcg
     3001 agcgtatgcc ggaagtgaat gaaattcttg agaatccgtt cggacacggt gcgttggagt
     3061 tgaaacgccg ctggcgggtc ggcagggatt tggggcggcg cggatacgat caggttatcg
     3121 tgttgcccgg ttctttgaaa tcggcagtca tcgcgctagc aacgggtatt gccaaaagga
     3181 cgggttatgt cggtgaaagc cgttattttc tgttgaacga tatacgcagg ctggataagg
     3241 aacgtctgcc tttgatggtg gacagatata cggctctcgc gcatccgagt caggaagatt
     3301 ttgacgggca ttcgggattc cccgagtttt ccattgatga acggcggcgg gaaatttctg
     3361 tcgaaacttt cggtttgaat cttggaaagc ctgttttggc attttgcccg ggcgcggaat
     3421 tcgggccggc aaagcgttgg ccgacaaggc attttgccga gttgggcaaa cattatctgg
     3481 cggcgggttg gcaggtttgg ctgttcggtt cgcaaaaaga tgatgaaatt gccgaggaaa
     3541 tcaactgcct ttcagacggt atgtgtgtca atttgtgcgg caaaaccgat ttgtcgcagg
     3601 caatggattt gctgtcgttg gcggacacgg ttgtgtgcaa cgacagcggg ctgatgcact
     3661 tggcggcggc attgggcagg aaggtggtgg cggtttacgg ttcttccagc ccgacgcata
     3721 cgccgccttt gagcgacagg gcgaaaatcg tcagcctgca cttggaatgt tcgccctgtt
     3781 ttaagcggga atgtccgctg gggcataccg actgcctcaa caggctgtat cccgagaaga
     3841 ttgtgcaggc ggttgaagag gcggtatgat gggttctgtt ttcgggttta tgccgtctga
     3901 cggcggatat tgataggatg tttatggcta ttgcgaacaa taaaaaagca tttcacgatt
     3961 ttttcattga agaccggatt gaagccggtt tggtcttgga aggctgggaa gtcaaagcaa
     4021 tccgcgccgc gcgcgtacag cttaaagaga gttatatcta ttggaaaaaa gacgcgtttt
     4081 atctggtcgg ctgccatatt acggctttgc ccacagcctc gacacacatt aaaccagatg
     4141 ccgtacgtcc gcgcaagctc ttgttgaacc agtcggaaat caacaaatta atcggcaaaa
     4201 ccgaacgtgc cggctacacc atcgtgccgc tggatttgca tttttcgcgc ggaaaaatca
     4261 agatggaaat cggtttggca aaaggtaaga aacagcacga caaacgtcag agtatgaaag
     4321 aggcggattg gaaacgcgag aaacagcggt tgattaagca tacgcgctga ttgtggaaac
     4381 agtgcttcag acggtatggg caaggctgcc gcctgatgtg taaacgttct acacctaaac
     4441 cagcatccgc tttaatcagc acagcatgat gccccaaacc ttcatcaaag gtttgagcaa
     4501 tttgatgata aattggcatt aaccaagggt aaagtgcggt catttttcct gaaatttttg
     4561 taaataacga gggcaattat acacattttc aaactggctt gacaaaatag caaaaagcaa
     4621 acgtttacct ttaataaata agaaataaat agagaaaaat gtgagctaaa tgacattttt
     4681 tgttcagttt tatgctaaaa tccacgccct tatttatttt tacttgacaa aggaaaattt
     4741 attatgaaac tcgaagcaag caagcagaag tttaaaaaat catttattat aagtctattt
     4801 ttttctattc tttatacctc tccgcttttg gctgttgatt acgtttacga caaaaccaag
     4861 ctcactgatg atgaaattac ccgcttaaaa aaactccgcg atagaaatag tgaatattgg
     4921 aaagatgatt tgttcaggat tgatatacca aaagaaactg gtctccgtca tgacattgag
     4981 ggggcagcaa aaggaaattt ttcgtatcca attttttata tcccaactga tgataaacct
     5041 attgatcctg acaaaaagat ctcttttttc gacagccctt atacccctgg ctacacggct
     5101 gctttcgtgc agggatttgg cgttaaagaa agaaatggct atacggaaga acaagctaaa
     5161 aaatatatcg atgaattgag aacaaaatta aaaaccgctc ctataatcgc agccgcccag
     5221 ctgggatatt ttcaattaaa tgatggaagc ggcgatagct ggctgtataa ttattatcgc
     5281 aatgatattt tcaattctta tggagagata aaacatagca atattaacag tgaaattctt
     5341 gctgttggta atgttgctga tttgtattat aaacacttca taagaaatcc atttatatat
     5401 gtagattggt ctatattctc tctccccctt cctagattaa aagtttccga aaattcccac
     5461 gttattgggc aaattattca tctttaccga ttagacttag aggattctct ttgggagcct
     5521 cgttgggatt ctgatgttag ttatttaaat ttattcaata gccatattcg ttttaatacc
     5581 aaaaatgaca gccttgttgt aggcgaaagc cgcattagac caacccccga taatgccttg
     5641 aataccgaaa aatatttaaa aagccaattt agcgatacag gttatcatag ttccgtgaga
     5701 agcacttata taggttatcc aagtattcgc tttgcttatg atttaagcga aagagaggct
     5761 gacaagcccg tattaacgtt aaaaagcaag gtaacaggga aaacagctat tgtttttgaa
     5821 gaaaaagcct taaacaattt aaaaaacctg acttaccgac agctcatcaa aacagaaacc
     5881 gatgttgaac cagatgcgtt ttttctttta gaagaatata aaaaaggcag atatcgttta
     5941 tttttgcgac aatgtccaaa cggtttttgt atcggtgtag aaaaactagc cattccaact
     6001 catcttgtcg cctcctatgc acaacaggct caagccgcca atactttatt caacctacgt
     6061 ttgaacgaca aaaacagcga tattttcgac cgcactttac cacgcaaagg cttgtggttg
     6121 cgtgtgattg acggacattc caaccaatgg gtgcaaggca aaacagcacc tgtagaaggc
     6181 tatcgtaaag gtgtgcaact cggcggcgaa gtattcacat ggcaaaacga aagtaatcaa
     6241 ctttccgttg gcttaatgag cggacaagca gaacaacgta gcactttccg caatccagat
     6301 acagacaatc ttacaacggg aaatgtgaaa ggctttggtg caggcgttta cgccacttgg
     6361 catcagcttc aggacaaaca gacaggtgcg tatgcggata gctgggtaca atatcaacgc
     6421 ttccgccacc gcattaacac tgaagatgct acagaacgtt ttacttcaaa aggtattacc
     6481 gcctcaattg aagcgggtta caatgcttta ttggcggaac acttcactaa aaagggcaac
     6541 agccttcgtg tttacctaca accacaggca caattgactt atttgggggt aaacggaaaa
     6601 ttcagcgata gcgaaaatgc ccacgtgaat ttacttggct cccgccaatt acaaactcgg
     6661 gttggcgttc aagctaaagc gcaattttcg ctctataaaa atatcgctat tgaacctttc
     6721 gccgctgtga acgcgcttta ccacaacaaa cctttcggtg tggaaataga cggagagcgt
     6781 cgagtgataa acaataagac ggcaattgag agccaattag gtgtagcagt gaaaatcaaa
     6841 tctcacttaa ctttacaagc aacattcaac cgccaaacag gcaaacatca tcaggctaaa
     6901 caaggtgcgt tgaatttaca gtggacgttt taagttaagt tgtaacaaaa taaaatcggc
     6961 agagaaattt actgccgatt tttttatttt aaaaatgcgg tcatttttcg tgtcatttcc
     7021 accaattttt taccgcactc acgagctcgc gctaaaccga caatcggatc tattgtccct
     7081 ttctttgttg tgtatgcaaa tcagaaatgc cggttacgga agcagtttgc ccggattcat
     7141 aatgttatat ggatcaaggt gttgtttgat gcttttcatc agggcgattt cggcaggcgt
     7201 gcgtactttg tccagccact gttttttgat gatacctatg ccgtgttcgg cggcaatcgt
     7261 gccgttgcag gcaagaacgt tgcgataaac tgcgtcgttg atgtcgtttt cgtaacgata
     7321 gacttcattg ctgaggattt cgggcaggaa agtattgtag tgcaggctgc cgtcgccaag
     7381 atgtccgaag cagacgattt gtatgccttt aaaattctgt tccaaatctt tggcgcaccg
     7441 gcggacaaag tcggcaacgc gtccgatagg aatggcaata tcgtgtttga tgctggtgcc
     7501 cagtttgcgt tgcgatgcgg agatgttttc gcgcaatgcc cacatatgga tacgttcttg
     7561 ttcgctttgt gccaatacgc tgtcggtaaa tcctttttta taaagaaatt cgacaagccg
     7621 atcatcaaga ttgctgtcgg gtaatgagtc ggtcaactca agtaaaatat gccattctga
     7681 atgtgtcggc agggggagtt tgctgaattc ggaagacaac tcggcggcaa aacggccgat
     7741 cagctcaaaa ctgcataggc gttcggcaaa gtgagcttgg gtttcggtca gcaggcggac
     7801 ggcggattcg atgtcgggta tgccgaccca tgccgttgcc ttgtccgagg gacgggcaaa
     7861 aagcttgagc gtggcggcag tgatgatgcc cagtgtgcct tcgctgccga taaacagatg
     7921 gcgcaggtcg tagccggtgg tgtttttatg caggggatgg agatgggaaa ccagctcgcc
     7981 gttggggagg acgacttcca aaccgataac caggtcgcgc atcgtgccgt aacgcaatac
     8041 gttcaaacct ccggcattgc aggcgatgtt gccgccgatt tgacaggagc cttcgctggc
     8101 gagactgagt gggaacagcc tgtttgaggc ttcggctgcc tgttggacgg tttggagtac
     8161 ggaacctgct tcgacggtta tgcagttgtc tgacaaattg atgctgcgga tgcggttgag
     8221 tttggaaagg ttcagcaata cgccgttttc cgatactgcc gctccgcaca aaccggtatt
     8281 gccgccttgc ggcgtaaccg gaatacggtg ttcgtaacaa aaacgcataa tggtttgcac
     8341 gctttcaacg ctgcgcggct gcaaaatgat gtctggtgca gacgtaaagc ggttgcgctg
     8401 gtcttttaaa agcgtcggag aaagttcggc aatttcatct gccggcagga gtcgggaaaa
     8461 ttcggtatga aggtcaggca tgtttgtcgg aagggtaggg agaagattga ggaaaggaat
     8521 ctgatgccgt ctgaaaaatc aaaaaagcag gatactgaac gtatcctgct tttttgcaag
     8581 cggaaaatgc ttattttgca gcttcggcag caggagcttc agcggcagga gcctcagaag
     8641 cggcagcttc agcggcaggt gcttcggtag cggcagcttc agcagcggga gcttcggcgg
     8701 caggtgcttc agtagcagga gcttcggcgg caggtgcttc agcagcggga gcttcggcgg
     8761 caggtgcttc agtagcggga gcttcggcgg caggtgcttc ggtagcggga gcttcagcgg
     8821 ctttttcacc gccgcaggct gccaggacca aagacaacaa agcagcggca aacagggatt
     8881 ttttcatttt agtaaccttt agaaccaatt taaattattg aaacaaatag gtctttatcg
     8941 accatactac aaaccgggtt ggaaattcat cgctgaattt gcgtaattat gcactatgga
     9001 ataagggatt gcaatgtcgt acagatggga aaaaggtttg aatgaaaagt tttctggcgg
     9061 gcggattgat aaatcctgta aaacggtttt gctgttaaaa attcgatagg gataaatatt
     9121 atagagatta atataatttc agtatcttgt atgatttatt aaggcggatt gggaaagtcg
     9181 gcagacaggt cggaatggtt ttaataatgt tgtaaaaaag ttacatcgca ataaaataac
     9241 aaaaatttac attgaaatat gtgctgtaca gtttatgaac aggttaacgg taatcggccg
     9301 gcgcggcggt tgtgttagaa tgcgcctgta atcaatctgc tgttcctgaa aggacaaata
     9361 tggctatcgt gaagaattcc gtggtttcgc tgcattatga gatgtatgat gccaacaatc
     9421 agcttttgga caaaaccgaa gaaccgattg cgtatctgca cggcggttac gacggcattt
     9481 tccctttggt ggaagaggcg ttgcacggta aggatgccgg cgatacggtc gatgtggcgc
     9541 tgtcgcccga cgatgcgttc ggcgagcagg atccggagtt ggtccgtatt gaagatgcgg
     9601 gcgtgttccc tgttgaagtc gaagtcggca tgatgtttga agccgacgat cttgaaaccg
     9661 gcgatgttgt cgtctatcgt gtaaccgatg ttgccgacgg taaggcggtg gtggacggca
     9721 accatccttt ggcaggcatg aaaatccgct ttaaggctac ggttgaaagt gtgcgcgatg
     9781 catccgatga ggaaatcgca cacggtcatg tccacggtcc gcacggtcat caccaccatt
     9841 gattgccggc agcttggagg tatctgatgc cgtctgaaaa gggtttgttc cgtttcagac
     9901 ggcattttgt ttgatggatg gagaaggtat gtcggttggg cattatgaaa attttcccgt
     9961 cggttcgctg attttgccgc gcaggttgag gaagccggtt catgcggtgt atgcgtttgc
    10021 acggacggcg gacgatatgg cggacgaggg cagtatgccg tctgaagcca ggttggcggg
    10081 gttggagggt ttgcggcgcg agttggacgt gttggcatcg ggcggccggt cggcgcatcc
    10141 tttgattgca cgattggatg ccgaggcggt tgtgccgttc ggtttggatt tgcagccgtt
    10201 ttatgatttg ctttcggcgt tttcgcagga cgtggttaaa acgcggtacg cgcatttcgg
    10261 cgagctggcc gattattgcc gacgttctgc caatcctgtc ggacgcatta tgctggcttt
    10321 gtacgggaaa acggatgcgg tgtgcgtggc gcaaagcgac ggcatctgta cggctttgca
    10381 actggtgaat ttttggcagg atgtggctgt ggattggcaa aagggcaggg tttatattcc
    10441 gcaggacgat ttgttgaaat tcggtgtttc cgaaggacag attgcggagg gtagggcgga
    10501 tgcggcgttt cagcggctga tggcgtatga gtgccggcgc gcatttcgta tgctgaaggc
    10561 gggttcgcct ttagcgcgcg aattgaaagg gcgtatcggt ttggaactcc gtatgattgt
    10621 gttgggggcg cagttgattt tgcagaaact ggacgcgtgc cgatacgatg tgtttgcaca
    10681 acgccccgtt ttggataaga aggattggtt gattatgctg aaacgcgcgt tgtggaaatg
    10741 atgggggaaa tgcatgccgt ctgaaagggg ttgttccgct tcagacggca ttttgcaggc
    10801 ggttggtatg gtcaggcgat ttcatgcggg ttcaacctgg cttgatcttc tgcacccaag
    10861 ccgatgagtt tgcgcagtcg ggtcatgtag aaattgacat ccaaaccggt gccgtagcgt
    10921 tgcgccgtcc acagtgtttc cgccagtgcc tccatcattt cgtgttcggc ttccagccag
    10981 ccgcgtttgg cgcacagggt gtcgtggatt gcgcgtatgc cgtgcggctg gtctatgccc
    11041 gcctgttctt ggacggacag atgcagcgac atatgcagga aggggttgct ttcgccgttt
    11101 tcgggcagcc agtcggtgtc cagatggtct tcgatgcgtt cgagataacg gtggtattcg
    11161 ggatgggctt cgataatgcg gagggctttc tgttccagtg cgctcagttg cagcggattg
    11221 agccgctgct gccacacgcg ggcgaaaaag cggcggacat cgtgggtatt gacatcgtac
    11281 atggcgggtt cggtaagatg tttatggaaa taggttcgga taatggcggg ttttacataa
    11341 attaaatccc accgtcaacc ttaccattta taatgcagac aaattatttg tctgaaacgg
    11401 attcgattat gaaaaaactg atttgcctgt tcgccgtatt tttgatgttg tgcggacgag
    11461 ctttcgcgct ggatgcgaac gatctgctgc cgccagaaaa ggcattcgtg ccggagcttg
    11521 ccgttgccga cgacggtgtg aacgtccgtt tcaggattgc cgacggatac tatatgtatc
    11581 aggcgaaaat cgtcggcaag accgatccgg cggatttgtt gggacagcct tctttcagca
    11641 agggcgaaga gaaggaagac gagtttttcg gcaggcagac ggtttaccat cacgaagcgc
    11701 aggttgcctt tccttatgca aaggctgtcg gcgaaccgta taaattggtt ttgacctatc
    11761 agggctgtgc cgaagccggc gtgtgctatc cgcccgtgga taccgagttt gatatttccg
    11821 gcaacggcac ttaccatccg caaaccgacg aaccggcatc cgccaaagac cgctttttgc
    11881 agccttcctc tcaaaacggc agcggggcgc tgccgccccc gaagggggat gagggcggcg
    11941 acagccgttt caagctgtct tgggatacgc tcaacgccaa tcttttggcg ttttttctcg
    12001 ctggtttggg cctgagtttt accgcctgta tgtatcccct gttgccgatt gtttccagta
    12061 ttgtggtcgg cgacaaaaag gcgggcaagg cgcgggcgtt tgtgctgtcc gtcgtttatg
    12121 ttcagggttt ggctctgact tatacgctgg tcggcattgt tgccggactg acgggcgcac
    12181 tgctgaccgt atggttgcag caggcttggg tggtattggc ggcatcggct ttaatggtcg
    12241 tcttggcact gtctatgttc gggctgttca acatccagct tcccaacgcc gtgcagtcgt
    12301 attttcagaa tcaaagcagc aggctttcag gcggtaaaat cgtttccgtc tttattatgg
    12361 gcatattgtc cgcgctgatt gtcgggccgt gcgtcgcccc gccgctggca tttgctttgg
    12421 gctacatcgg tcagacgggc gatgcggttt taggcggttt ggcactttac actttggcgt
    12481 tgggcaccgg cgttccgctg attgccatcg gcacgttcgg cgggcatatc ctgcctaggg
    12541 caggcgattg gatgaatgcc gtcaaatacg ctttcggctt tatcctgctt gccgtcgccg
    12601 tttacctcgc cacgccgcac ttgccctatt atctcgtcgt cgcgctgtac acgctgctga
    12661 tgctggttcc tgcctgtatg ctgctggtca acggacgcag gcagaaacgc cgtccgaaag
    12721 ctgtggcatt cgcattgggc ggcatattgc tgataggcgg cgcgtggttc ggctggcagg
    12781 gcgcaaacgg caaaacgacc gcgctgcacc atttcctgac cctcaatccg ccggccgaag
    12841 caggcaaatc ttcggaacac ggaaaaatgt ttgccgatac tgccgcgctg aaggcagcga
    12901 tggatacggc gttgaaagaa catcccgaca aacccgtcgt tttggatttt tatgccgact
    12961 ggtgcatttc ctgcaaagaa atggcggctt acacgctcaa tcagccggaa gtgcatcagg
    13021 cagtcgatat ggaacgcttt ttccaaatcg acgtaaccgc caacacgccc gaacatcagg
    13081 cgttgttgaa agaatacggt ctgttcggac cgccgggcgt gtttgtcgtc cgctccgacg
    13141 gcagccgcag cgagccgctg ctgggttttg tcaaagcaga caagtttatc gagtggtatg
    13201 aacaaaaccg ctgattccga tgtttcaaat gccgtctgaa gttctcagac ggcatttttg
    13261 tcagaaagga aaacggcaat atccgctata cacacggaag cagtccaaag atgtgaaaac
    13321 cgtgtgtata agttagtttg cgtattgaaa ttaaaagata aaaatacgat gattaaaaaa
    13381 taggcataag aaaagataaa cgcagccggt gtttgccggt gtgtgtatct tttgtggaaa
    13441 aacatcttaa tgtcatgtat tttatggaaa ataataacat gattaaaaaa tagtcaatca
    13501 ttaaggttca ggtgggcggc tgcaaccgtc aatgtctgaa aagaggcagt gctgtttaag
    13561 tatggaaagt cggatatagt ggattaacaa aaatcaggac aaggcgacga agc
//
LOCUS       DAXHSF010000038        13376 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.038, whole genome shotgun sequence.
ACCESSION   DAXHSF010000038 DAXHSF010000000
VERSION     DAXHSF010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13376
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.038"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(100..528)
                     /locus_tag="ACFM8S_000844"
     CDS             complement(100..528)
                     /locus_tag="ACFM8S_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HFC6313939.1"
                     /translation="MAILKLDEHLYISPQLTKADAEQIAQLGIKTVICNRPDREEESQ
                     PDFAQIKQWLEQAGVTGFHHQPVTARDIQKHDVETFRQLIGQAESPVLAYCRTGTRCS
                     LLWGFRRAAEGMPVDEIIRRAQAAGVNLENFRERLDNARV"
     gene            complement(593..2341)
                     /locus_tag="ACFM8S_000845"
     CDS             complement(593..2341)
                     /locus_tag="ACFM8S_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HFC6313940.1"
                     /translation="MIHYKITSSPLDHEWHILLTFTQDDDLPIEISLPNWVPGSYLIR
                     DFSRHITSIHASCNGTSMPLEQIAKNRWHAAAVRGEWQIRYTVYAFDLSVRGSFLTTE
                     RGFFDGSCLFLKVEGTETLPHRLELTGIPSEWRIATTLPETGRFVFQAASYAELIDRP
                     VEMGLIEFLDFEAAGIPHTIALSGIYPDFDRNRLVSDIKKICETELAVFSSPAPFQKY
                     LFLLHVGDHIYGGLEHTDSTALLADRHSLPPYGMTDADDTYTTLLGLFSHEYFHAWNV
                     KSIKPAAFVPYDLDKENYTEQLWAFEGITSYYDDLFLARSRTISPESYLNLLAQGITR
                     VQQTRGRLRQTLAESSFTAWNKFYKPDENSPNAIVSYYQKGALAALCLDLIIRNRSNG
                     RHSLDTLMDKLYREWRDTHSGIPEKHWQIRCQEITGLDLEDFFQKALYSTEDLPLAEC
                     LATAGVGLTFLPLPRQHGGGYAEHICPVPPAGDFGARFKQNTDHIVLTHVFNSGSAES
                     AALCPQDKIIALDGYACTDFTAQWARYHVNAKINIHFFRAGILRQTVLTVQAAAADTA
                     ILHITDRNLLDNWLFG"
     gene            2668..4101
                     /gene="ccoN"
                     /locus_tag="ACFM8S_000846"
     CDS             2668..4101
                     /gene="ccoN"
                     /locus_tag="ACFM8S_000846"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045278 - plasma membrane respiratory
                     chain complex IV [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HFC6313941.1"
                     /translation="MDTQTYNYKVVRQFAIMTVVWGIVGMLVGVIVAAQLFAPALDLS
                     NIGPWFHFGRLRPLHTNAVIFAFGGCGLIGTSYYVVQRTCNTRLFGGWLPAFTFWGWQ
                     AVIVAAVVSFPMGWTQGKEYAELEWPIDILITLVWVAYAIVFFGTIAKRKIKHIYVAN
                     WFYGGFILAVALLHIVNNISIPAGLMKSYPVYSGAIDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAARPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVLSL
                     ILFAPSWGGMINGIMTLSGAWDKLRTDPILKFLIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTVAHVHAGALGWVGFVTIGSVYYMIPRLFGKEQMHSTKLVEAHFWIATIGVV
                     LYIAAMWIAGVMQGLMWSSLNDDGTLTYSFVESVKRTMPYYVIRFAGGLLYLSGMCIM
                     AYNVYRTAIGGKAVDAEIPAVSQTQHH"
     gene            4128..4739
                     /gene="ccoO"
                     /locus_tag="ACFM8S_000847"
     CDS             4128..4739
                     /gene="ccoO"
                     /locus_tag="ACFM8S_000847"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HFC6313942.1"
                     /translation="MKLQQLAEEKIGVLIVFTLLVVSVGLLIEVVPLAFTKAATQPAP
                     GVKPYNALQVAGRDIYIREGCYNCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGS
                     KRTGPDLARVGGRYSDEWHRIHLLNPRDVVPESNMPAFPWLARNKVDVDATVANMKAL
                     RKVGTPYSDEEIAKAPEALANKSELDAVVAYLQGLGLALKNVR"
     gene            4744..4914
                     /locus_tag="ACFM8S_000848"
     CDS             4744..4914
                     /locus_tag="ACFM8S_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HFC6313943.1"
                     /translation="MDINGIRALFTVWIFICFLLVLYIVFNRRNKKNYDDAANSIFDE
                     NQDAQDKKSENR"
     gene            4938..5849
                     /gene="ccoP"
                     /locus_tag="ACFM8S_000849"
                     /pseudo
     CDS             4938..5849
                     /gene="ccoP"
                     /locus_tag="ACFM8S_000849"
                     /inference="COORDINATES: protein motif:HMM:TIGR00782.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
     gene            5964..6269
                     /locus_tag="ACFM8S_000850"
                     /pseudo
     CDS             5964..6269
                     /locus_tag="ACFM8S_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
     gene            6471..7880
                     /locus_tag="ACFM8S_000851"
     CDS             6471..7880
                     /locus_tag="ACFM8S_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HFC6313944.1"
                     /translation="MDTQIKTEADNQSNRRYLTVWRWHFYAGLLVMPFLTLLAVTGLG
                     MLLFANITGKEGERIHVVPQATVQPLSVQAEAARSAVNPETSSVVQYIAPRADDMVAV
                     FRVNNEGKATMVAVDPYTAKVVSTMPRNQGWYYAMDEIHSDMMLGSTGDYLLETAASL
                     TIIMIISGLYLWWVKRRGIKAMLLPPKGRARSWWRNLHGAFGTWVSLILLLFCLSGIA
                     WAGIWGGKFVQAWSQFPAGKWGVEPNPVSVVPTHGEVLNDGKVKEVPWILELTPMPVS
                     GTTVGKDGINPDEPMTLETVDRFAREIGFKGRYQLNLPKGEDGVWTLSQDSMSYDMIS
                     PFADRTVHIDQYSGKILADIRFDDYNPFGKFMAASIALHMGTLGWWSVLANVVFCLAV
                     IFIGISGCVMWWKRRPSGAVGMVPPAQKIKLPVWWAMAAPLLLIALLFPTALLAIAVI
                     WLLDTLLLSRIPVLRRWFK"
     gene            7981..11190
                     /gene="recC"
                     /locus_tag="ACFM8S_000852"
     CDS             7981..11190
                     /gene="recC"
                     /locus_tag="ACFM8S_000852"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFC6313945.1"
                     /translation="MFYLYQSNRLETLAALFARIQKVKPLKSALQPEQIIVQSQGMRR
                     YLNTCLARDLGVAANLSFSLPAGLTWKLMKKLIPGIPELSPFAPEVMRWRLLDLFRSE
                     AFRNTAEFEDVRNVLQDYLGSGESADYQLAGQLADIFDQYLVYRPQWIDAWQQGRRLG
                     LGDDEIWQSKLWRYLDDGRQSAPHRVALWEKLLESLSSDKLPERYFVFGISTMAPMYL
                     QLLHKLSEHCDVFVFALNPSGMYWGNVIEAAQILKGGGDPDLTQAGHPLLASLGKQGR
                     DFFDFLNEMEIEGETPVFEEGGRDTLLHALQTDIQNLKMPSENVGSVGTDDGSIRIVS
                     AHSPLRELQILKDKLLKILHEHPDWQPHDIAVLTPNIESYTPFIEAVFGQAQPGAQAL
                     PYSVSDVKISRRQPLFHALSCLFDLLESRFEVDKVLVLLETAPVLRRFGLTEDDLPLL
                     HDMVADLNVHWGLDGEMRGGTDQLFTWKQAVERMILGWMLPEGGNPMWQDVSAWYADV
                     NQTALFGRFAALLETLSDIVRIWQQPATVGEWVARCRDLLETLFQTEADDQKAVQNLE
                     NEWVKWQAETELAQFSGQLPPHTVIRHIRRFLDSESEAGFLRGGITFCSMVPMRSLPF
                     KVICLLGLNDGDFPRNTKAAVFDLIAKHPAKGDRARRDDDRYLFLEALISAREILYLS
                     YIGRDIRKDEELAPSSLLGELIDTVAAMTGTNSRQLAQNLIEQHPLQAFSRRYFQEGG
                     RSDGIFGTRTDYAAALGQTPEPPQPFFDQPVENAEPVAEIGQDEFIRFWRNPVKVWLQ
                     QQLAWSEPHIGEAWEPAEPFEPQHADQIAKTYIEARREGRDFSQTAARIGAESLLPSG
                     ELGRLWQQDFQTAAKQIDTAVLNSPKLPPLSYAIPSDGQILKGSLGNLYRCGQVFYAY
                     GKPNAPQRIAFLLEHLIFCAVMPSETEARQTFIVQSGETEILAEIAQDRALQLLSEWM
                     AFFNIGQNRPLPFFAKTSLAAAEAFAQKQDWEAALKKAQTAYHGNKVSKGQKDYTEVA
                     LVFGNASQNPLEQPLFENLVRLLADTFAAAEKKEEAGEA"
     gene            complement(11258..12826)
                     /gene="mtrF"
                     /locus_tag="ACFM8S_000853"
     CDS             complement(11258..12826)
                     /gene="mtrF"
                     /locus_tag="ACFM8S_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041421321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family antimetabolite efflux transporter
                     MtrF"
                     /protein_id="HFC6313946.1"
                     /translation="MSQTDTQRDGRFLRTVEWLGNMLPHPVTLFIIFIVLLLIASAAG
                     AYFGLSVPDPRPVGAKGRADDGLIHVVSLLDADGLIKILTHTVKNFTGFAPLGTVLVS
                     LLGVGIAEKSGLISALMRLLLTKSPRKLTTFMVVFTGILSNTASELGYVVLIPLSAII
                     FHSLGRHPLAGLAAAFAGVSGGYSANLFLGTIDPLLAGITQQAAQIIHPDYVVGPEAN
                     WFFMVASTFVIALIGYFVTEKIVEPQLGPYQSDLSQEEKDIRHSNEITPLEYKGLIWA
                     GVVFVALSALLAWSIVPADGILRHPETGLVSGSPFLKSIVVFIFLLFALPGIVYGRVT
                     RSLRGEQEVVNAMAESMSTLGLYLVIIFFAAQFVAFFNWTNIGQYIAVKGATFLKEVG
                     LGGSVLFIGFILICAFINLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDS
                     VTNIITPMMSYFGLIMATVIKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLG
                     LPVGPGAPTLYPAP"
     gene            13160..13372
                     /locus_tag="ACFM8S_000854"
     CDS             13160..13372
                     /locus_tag="ACFM8S_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313947.1"
                     /translation="MKNEIQKIMDKYNPWHEDDFESYEDIAKDVSLMTDKTFIEHYLL
                     EVYSEENGHFDQENVHAMIEEIKNAI"
BASE COUNT         3339 a         3223 c         3557 g         3257 t
ORIGIN      
        1 actgtaaaaa cggagaagga cagaggacgg cagtaaggtt tgccgtcctc tttttcaagt
       61 tgcggtggaa tgccgcttga aggtgttttc ggcttgtaat cagacgcggg cgttgtccag
      121 ccgctctctg aagttttcca aatttacgcc tgccgcttgg gcgcggcgga tgatttcgtc
      181 aaccggcata ccttctgccg cccggcggaa gccccacagg agggagcagc gcgtaccggt
      241 ccggcaatag gcaaggacgg gagattcggc ttgtccgatg agttggcgga aggtttcgac
      301 atcgtgtttt tggatgtcgc gtgcggtaac gggttggtga tggaatccag taacgcctgc
      361 ttgttccagc cactgtttga tttgggcgaa gtcgggttgc gattcttctt cgcggtcggg
      421 gcggttgcag atgacggttt tgatgcccag ttgcgcgatt tgttccgcat cggctttggt
      481 cagttgcgga gaaatataga ggtgttcgtc aagtttcaga atggccattt tgtttccttt
      541 actttgcggt tgtttttcag acggcatttt gtgtgcaatg ccgtctgaaa gtttaaccga
      601 acaaccagtt gtccaacagg ttccggtctg tgatatgtag gatggcagta tccgctgccg
      661 ctgcctgaac cgtcaagacg gtttgacgca atatgccggc acggaagaag tggatattga
      721 tttttgcatt gacgtggtat cgggcccatt gtgcggtaaa gtcggtgcag gcataaccgt
      781 ctaaagcaat gattttgtct tgcgggcaca gtgccgcaga ttccgcgctg ccgctgttga
      841 agacatgggt caggacgatg tggtcggtgt tttgtttgaa acgtgcgcca aaatcgcctg
      901 ccggcgggac ggggcagatg tgttctgcgt atccgccgcc gtgttgtcgg ggaagtggca
      961 ggaaggtcag tcccacgcct gcggttgcca ggcattcggc aagcggcaaa tcttcggtac
     1021 tgtataacgc tttttggaaa aaatcttcca aatccaagcc ggtaatttcc tgacagcgga
     1081 tttgccagtg tttttccgga atacccgagt gtgtgtccct ccactcccga tagagtttgt
     1141 ccattaacgt atcgagagaa tgtctgccgt tgcttcggtt gcgtattatc agatcaaggc
     1201 acaatgcggc aagcgcgcct ttctggtagt agctgacgat ggcgttgggg ctgttttcat
     1261 ccggtttgta aaatttgttc cacgcggtaa aactcgattc cgccaaggtc tgcctcaaac
     1321 ggccgcgggt ttgttgtacg cgcgtaatgc cttgtgccag caggtttaaa taagattcgg
     1381 gcgagatggt gcggctgcgt gccaaaaaca aatcgtcgta ataggatgta atgccttcga
     1441 atgcccatag ttgttcggta tagttttctt tgtcgaggtc ataagggacg aacgcggcag
     1501 gtttgatgga tttgacgttc cacgcgtgaa aatattcgtg ggagaaaagt ccgagcaatg
     1561 tggtgtaggt atcgtcggca tcggtcatac cgtacggcgg aaggctgtgg cggtcggcga
     1621 gcagggcggt gctgtcggtg tgttccaaac cgccgtaaat atggtcgccg acgtggagca
     1681 ggaacaaata tttttgaaac ggggcagggg aggaaaacac cgccagttct gtttcgcaga
     1741 tttttttgat atccgaaacc agcctgttgc ggtcgaaatc gggatatatg ccgcttaagg
     1801 caattgtgtg cggaatgcct gccgcctcaa aatctaaaaa ttcaatcaag cccatctcga
     1861 caggtcggtc aatcaattcg gcataagatg ccgcctgaaa gacaaacctc cctgtttccg
     1921 gcagcgttgt ggcaatacgc cattcggacg gaatacccgt caattccaag cggtgcggca
     1981 gcgtttccgt tccttcgact ttcaaaaaca ggcacgatcc gtcaaaaaaa ccgcgttctg
     2041 tcgtcaggaa agaacctcga accgacaaat cgaatgcata tacggtgtag cggatttgcc
     2101 actcgccgcg tacggcggca gcgtgccagc ggttttttgc aatttgttcg agcggcatgg
     2161 acgtgccgtt acaggatgca tggatagaag tgatgtggcg ggaaaaatcc cgaatcagat
     2221 agctgcccgg aacccagttt ggcaggctta tttctatagg aagatcatca tcttgtgtga
     2281 atgtcagcag gatgtgccac tcgtggtcga gaggggacga ggttatttta taatgaatca
     2341 taactatcaa atatatcgtt aaacgggatg gattaaatac ctttaaaatg cagcttaacc
     2401 tacatcaata aagtgccaca aaaagggaaa attggaaaca aaagcaatgg cggtcgggtt
     2461 gtaatttatt ataacaatat gatttatatg taataaaaaa cagattaagc aaagagctcg
     2521 ccggcggcgc gaatgtaatt ccgcttggcg taataaggca gatatgttaa atttagaaaa
     2581 aattacgctt gcccggattg caccttaaac aggctgcgga agcggcagga taaaaatctt
     2641 tattcccata aattcatgga gactctcatg gacacacaaa cttacaacta caaagtggtg
     2701 cgccagttcg ccatcatgac tgtagtttgg gggattgtgg gtatgttggt cggcgttatc
     2761 gtcgccgccc agctttttgc tcctgccctc gacttgtcta atatcggacc ttggttccac
     2821 ttcggccgcc tgcgtccgct gcacaccaat gcggttattt tcgcatttgg cggttgcggc
     2881 ctgatcggca catcatacta cgttgtccaa cgtacttgta atacccgtct tttcggcggt
     2941 tggctgccgg catttacctt ttggggctgg caggcggtaa tcgttgccgc cgtcgtcagc
     3001 ttccctatgg gttggaccca aggtaaggaa tatgccgaac tggaatggcc gatcgatatt
     3061 ctgattactt tggtttgggt ggcttacgcc atcgtattct tcggtacgat tgccaaacgt
     3121 aagattaaac atatttacgt tgccaactgg ttctacggcg gctttatttt ggcggtcgca
     3181 cttttacaca tcgtcaacaa tatcagcatc ccggccggtt tgatgaagtc ataccccgtc
     3241 tattccggtg cgattgatgc tatggttcaa tggtggtacg ggcataacgc cgtgggcttc
     3301 ttcctgactg ccggcttctt gggtatgatg tactatttcg tacccaaaca agcagcccgc
     3361 cccgtttact cctaccgcct gtccgtcgtt cacttctggg cgttgatttt cacctatatg
     3421 tgggcgggtc cgcaccatct tcactacact gcgctgcctg actggacgca atctttgggt
     3481 atggttctgt ctttgattct gttcgcaccc tcttggggcg gtatgattaa cggcatcatg
     3541 accttgtccg gcgcgtggga caaactgcgt acagacccga ttcttaaatt cctgattgta
     3601 tccttgtcct tctacggtat gtctaccttt gaaggcccga tgatgtcgat taaaacggtc
     3661 aatgcattga gccactatac ggactggacc gtcgcgcacg ttcatgcggg tgcgttgggc
     3721 tgggtaggct ttgtaaccat cggttccgtc tattacatga ttccccgtct gttcggcaaa
     3781 gaacagatgc acagcaccaa gctggtagaa gcacattttt ggattgcgac catcggcgtg
     3841 gttctgtata tcgctgccat gtggattgcc ggtgtgatgc agggtttgat gtggagttct
     3901 ttgaacgatg acggtacgct gacctactcg tttgtcgaat ccgtaaaacg caccatgcct
     3961 tactacgtga tccgttttgc aggtggttta ttgtatctga gtggtatgtg cattatggcg
     4021 tacaacgtgt accgcacagc catcggtggt aaagcagtcg atgccgaaat ccctgcggtt
     4081 tcccaaacac agcaccacta aaatactaag aaagataggc taccaaaatg aaattacaac
     4141 aattggctga agaaaaaatc ggcgttctga ttgtgttcac gctgcttgta gtcagtgtcg
     4201 gtctgttgat tgaagttgtg cccttggcct ttaccaaggc ggcaacacag ccggcgccgg
     4261 gcgtgaagcc ttacaatgcc ctgcaggttg ccggacgcga tatttacatc cgtgagggct
     4321 gttacaactg ccactcgcaa atgattcgtc cgttccgtgc ggaaaccgag cgttacggtc
     4381 attactctgt tgccggagag tcggtttacg accatccgtt ccaatggggt tccaaacgta
     4441 ccggtcctga tttggcacgt gtgggcggtc gctattccga cgaatggcac cgtatccacc
     4501 tgctgaatcc ccgtgatgtc gtgcctgagt ccaatatgcc ggcattcccg tggcttgcac
     4561 gcaataaagt cgatgtcgat gcaaccgttg ccaacatgaa ggctttgcgt aaagtaggta
     4621 ctccttacag tgatgaggaa attgcgaaag cgcctgaggc tttggcaaac aaatccgagc
     4681 tggatgctgt agtcgcctat ctgcaaggat tgggtctggc tttgaaaaac gtaaggtaac
     4741 atcatggata ttaacggtat tcgtgctctc ttcacggtat ggatctttat ctgtttcctg
     4801 ttggtactct atatcgtctt caacaggcgg aataagaaaa actacgatga tgccgcaaac
     4861 agcatttttg atgaaaacca agatgcgcaa gataagaaaa gcgaaaaccg ttaatattgt
     4921 gataacggag caaaacaatg aacacaacat cccaatttac cagtaatttc tggaatatat
     4981 atattgcagt tattgtctta ctgagcttta tcgctttggc ttggctgctg ctgtctcaaa
     5041 atgttgtcaa acgtccgaag aagggcgaag aagtacaaac tacgggtcat gagtgggacg
     5101 gcattgccga atacgacaat ccgctgcccc gctggtggtt ttggctgtgt gttttgacgt
     5161 ggctgttcgg tatcggttat ttggttatgt atccgggtgt cggcgactac aaaggtctgc
     5221 tgaaatggac cagccataac caatatgaaa aagaggtcaa aaaagccgat gagcaatacg
     5281 gcaaactgta tgccaagttt gcggatatgc cgattgaaaa agtggcaaaa gaccctcaag
     5341 ccaagcaaat cgcccaaaac ctgtttaaca cttattgtat ccagtgccac ggctctgatg
     5401 ctaaaggctc taaaggtttt ccgaatctga ccgatagcga ttggttgtgg ggcggtgatc
     5461 ccgataaaat ccacgagacc atcgaaaaag gccgtgttgc gactatgcct gcctggggcc
     5521 ctgctttggg cgaagaaggc gtgaaagacg ttgcccatta tgtgatgtcc ctttcaaaac
     5581 ccgaaggtca gtatgatgag gaacgtgccg cgcgcggaca agccttgttc agcggtccgc
     5641 ctgccaactg tttcacttgc cacggcgata agggacaagg tatccaaggt ttgggtccga
     5701 acctgactga tgacgtgtgg ttgtggggcg gtacgcaaaa atccattatc gaaaccatta
     5761 ccaacggtcg cagcagccaa atgcccgctt ggggacattt cttggataaa gacaaactgc
     5821 atattatgac tgcttatgta tggggtcttt ccgataaaga cggtaaagct ccggtgaaaa
     5881 aagccgagcc tgcaccgact cccgcaccgg cggcagaacc cgcagcctct gctcctgcag
     5941 aagcagcaca agccgcgtcc gaagccaaac ctgccgcagc agaacctaaa gccgaggaaa
     6001 cagccgcacc tgctgccaaa gcggacggca aataggttta tgaaaccgtt tgtgccgcct
     6061 gccatggcaa tgcgattccg ggtattcccc atgtaggcat caaagccgat tgggccgacc
     6121 gcatcaaaaa aggcaaggac acgttgcaca aacacgcgat cgaaggtttc aatacgatgc
     6181 ccgcaaaagg cggtcgcggc gatttgagcg atgatgaggt taaagctgcg gttgactata
     6241 tggtcaacca gtcaggcggc aaattctaac ttgactgagt ttccgatatt atcggttatg
     6301 aaaatccaga ttcagtattg aatctggatt ttttgtttta acgggcatcc ggttttaaac
     6361 gcagactgcc tatttgttgt aatgaatttt gactttagtc ataaagaatt agtctttatt
     6421 gtttataatc gcgcatcatc acgatagccc tattttcaaa aggacgatta atggatacac
     6481 aaatcaaaac tgaagccgac aatcagagca accggcgtta tctgaccgtt tggcggtggc
     6541 atttttatgc cggtctgctg gttatgcctt ttcttaccct gcttgccgtt acgggtttgg
     6601 gtatgctgct gtttgccaat attaccggta aggagggcga gcggattcat gttgtgccgc
     6661 aggcaacggt acaacctctg tctgttcagg cggaagcggc acgcagtgcc gttaatccgg
     6721 aaacttcgtc cgtcgttcag tatattgcgc cgcgtgccga tgatatggtt gccgtgttcc
     6781 gtgtcaacaa tgagggcaag gcaacgatgg tcgcggtcga tccttatacg gcaaaagtgg
     6841 tcagtaccat gccgcgcaat cagggttggt attacgcgat ggatgaaatc cacagcgata
     6901 tgatgctcgg ttcgacaggt gattatcttt tggaaacggc tgcatcgctg acgattatca
     6961 tgataatcag cggtttgtac ctttggtggg tgaaacggcg cggcatcaag gcgatgctgc
     7021 tgccgccaaa aggcagggcg cgttcttggt ggcggaatct gcacggcgcg tttggaactt
     7081 gggtgtcgtt gattttactg ttgttctgcc tgtcgggtat tgcttgggca ggtatttggg
     7141 gcggcaagtt cgtgcaggct tggagtcagt tcccggcagg caaatggggt gtcgaaccga
     7201 accctgtttc agtcgtgccg acccatggcg aggtattgaa cgacggcaag gttaaggaag
     7261 tgccgtggat tttggagctt acgcctatgc ctgtttcagg gacgactgtg ggcaaagacg
     7321 gcattaaccc cgacgagccg atgacattgg aaaccgtcga ccgttttgcg cgggaaatcg
     7381 gtttcaaagg gcgttatcag ttgaatttgc ccaaaggcga ggacggcgta tggactttgt
     7441 cgcaagactc tatgagttac gatatgatta gtccgtttgc cgaccgcacg gtgcatatcg
     7501 accagtacag cggcaagatt cttgccgaca tccgttttga tgattacaac ccgttcggca
     7561 aatttatggc ggcgagcatt gcgctgcata tggggacttt gggctggtgg agcgtgttgg
     7621 cgaacgtcgt gttctgcctt gccgtcattt ttatcggtat cagcggctgc gtgatgtggt
     7681 ggaaacgccg tccgtccggc gcggtgggca tggttccgcc ggcgcaaaaa atcaagctgc
     7741 ccgtctggtg ggcaatggcg gcgccgctgc tgctgattgc attgcttttc ccgaccgcgc
     7801 tgcttgccat tgccgtgatt tggctgttgg atacgctgct gttgtcgcgg attcctgttt
     7861 tgaggagatg gtttaaatga cccgatgccg tctgaagcgt tcagacggca ttttgtttga
     7921 aggcggacgg gggaaaggct atataatccc gaatactttt ccgcaacttt tgtcgaaatc
     7981 atgttttatc tgtatcaatc caaccgtctt gaaacgctgg cggcattgtt tgcccgcatt
     8041 caaaaagtca aaccgctgaa atcggcttta cagcccgaac agattattgt gcagagtcag
     8101 gggatgcgcc gctatctcaa tacctgcctc gcccgcgatt tgggcgtggc ggcgaatttg
     8161 tcgttcagcc tgcccgccgg cctgacgtgg aagctgatga aaaaactgat tcccggtatt
     8221 ccggaactca gcccgtttgc acccgaagtc atgcgctggc ggctgctgga tttgttccgc
     8281 agtgaagcat tccggaatac ggcagaattt gaagatgtga ggaatgtgct gcaagactat
     8341 ctgggcagcg gcgaatcggc agattaccag cttgcgggac agcttgcgga catattcgac
     8401 caatacctcg tgtaccgtcc tcagtggata gacgcttggc agcagggcag gcggctcggt
     8461 ttgggcgacg acgaaatctg gcagtccaaa ctgtggcgtt acctcgacga tggcaggcag
     8521 agcgcgccgc accgtgtcgc gttgtgggaa aagctgttgg aatctttgag cagtgataag
     8581 ctgcccgagc gttatttcgt gttcggcatt tccacgatgg cgccgatgta tttgcaactt
     8641 ttgcacaagc tgtccgaaca ttgcgacgtg ttcgtgttcg cactcaatcc gagcgggatg
     8701 tattggggca atgtcatcga ggcggcgcaa atcctcaaag gtggcggcga tcccgattta
     8761 actcaggcag ggcatccgct gctcgcctca ttgggcaagc aggggcggga ctttttcgac
     8821 tttttgaacg aaatggaaat agaaggcgaa acgccggtat ttgaagaagg cggacgcgat
     8881 acgcttttgc acgccctgca aaccgatatt caaaacctga aaatgccgtc tgaaaatgtg
     8941 ggaagcgtcg gtacggatga cggttcgata cgtatcgtct ccgcacacag ccctttgcgc
     9001 gaattgcaga tactcaaaga caaactgttg aaaattctgc atgaacatcc cgattggcag
     9061 ccgcatgata tcgccgtatt aaccccgaac atcgaatcct atacgccttt tatcgaagcc
     9121 gtgttcggac aggcgcagcc cggtgcgcag gcattgccgt attccgtttc agacgtgaaa
     9181 atcagccgcc gacaaccgct gtttcatgct ttgtcatgcc tgttcgactt gttggaaagc
     9241 cgatttgaag tcgataaagt gcttgtgctt ttagaaaccg cacccgtgtt gcgccgtttc
     9301 ggactgactg aggacgattt gccgcttttg cacgatatgg ttgccgattt gaacgttcac
     9361 tggggtttgg acggagaaat gcgcggcggc acggatcagc tgtttacctg gaagcaggcg
     9421 gtagaacgca tgatattggg ctggatgctg cccgaaggcg gcaacccgat gtggcaggat
     9481 gtcagcgcgt ggtatgccga cgtgaaccaa accgccctgt tcggacgttt tgccgccctc
     9541 ctcgaaaccc tgtcggatat tgtacggata tggcagcaac ctgcgacggt cggcgaatgg
     9601 gtggcgcgtt gccgggattt gcttgaaaca ttgttccaaa ccgaagccga tgaccaaaag
     9661 gcagtccaaa acctcgaaaa cgaatgggtc aaatggcagg cggaaaccga attggcgcaa
     9721 ttttccggac agttgccgcc gcacaccgtc atccgccaca tccgccgctt tctcgacagc
     9781 gaaagcgagg caggcttttt acgcggcggc atcacctttt gcagtatggt gccgatgcgg
     9841 agcctgccct ttaaagtcat ctgtctgttg ggtttgaacg acggagattt tccccgcaat
     9901 accaaagccg ccgtattcga cctgattgcc aaacatcccg ccaaaggcga ccgagcccgt
     9961 cgcgatgacg accgctacct gttccttgaa gctctcatca gcgcgcgtga aatcctgtat
    10021 ttgtcctaca tcgggcgcga tatccgcaaa gacgaagagc ttgccccgtc ttcgctgttg
    10081 ggcgaactca tcgacacagt tgccgccatg accggcacaa acagccgcca acttgcacaa
    10141 aacctgatag aacagcatcc gctgcaagcc ttctcgcgcc gatattttca agaaggcgga
    10201 cgttcagacg gcatattcgg cacgcgtacc gactatgccg ccgcgctcgg acaaacgccc
    10261 gaaccgccgc aacccttttt cgatcaaccc gtagaaaacg ccgaacctgt tgccgaaatt
    10321 ggacaggacg aatttatccg cttctggcgc aaccctgtca aagtatggct tcagcagcag
    10381 cttgcgtgga gcgaacccca tatcggcgaa gcctgggagc ctgccgaacc cttcgagccg
    10441 caacacgccg atcaaatcgc caaaacctac atcgaagcac gccgcgaagg acgggatttt
    10501 tcccaaaccg ccgcccgcat cggggcggaa agcctgctgc cgtcgggaga gttgggcaga
    10561 ctttggcagc aggacttcca aaccgctgcc aaacaaatcg acacggcggt tttaaacagc
    10621 cccaaactgc caccgctttc atatgccata ccgtcggacg ggcaaatcct gaaaggcagc
    10681 ttgggcaatc tgtaccgctg cggacaagtg ttttacgcct acggcaaacc caacgcgccg
    10741 caacgtatcg cttttctgct ggaacacctg atattttgcg ccgttatgcc gtctgaaacc
    10801 gaagcgcggc aaacctttat cgtccaatcc ggagaaaccg aaatattggc ggaaatcgcg
    10861 caagacaggg cattgcagct attgtcggaa tggatggcgt ttttcaatat cgggcaaaac
    10921 cgcccgctgc cgttttttgc caaaacctcg cttgccgccg ccgaagcgtt tgcccaaaaa
    10981 caagattggg aagccgccct gaaaaaagcc caaaccgcct accacggcaa caaagtcagc
    11041 aaagggcaga aagactatac cgaagtcgcc ctcgtgttcg gcaacgcaag ccaaaacccg
    11101 ctcgaacagc ccctgtttga aaacctcgtc cgcctgcttg ccgacacgtt tgccgcagcg
    11161 gaaaaaaagg aagaggccgg agaagcctga cgggatggaa aggcaaatgc cgtctgaaag
    11221 cgtttaagca tttcagacgg cattttgttt atcgtgttta aggtgcggga tacaaagtgg
    11281 gtgcgccggg accgacgggc aggcccaaaa caaataccca aatgcagaat aaggcaatcc
    11341 atgcaattaa gaagaaagcg gaatacggca acatcataga aatcagcgta cccacgcccg
    11401 catctttttt gtatttgatc accgtcgcca taatcagccc gaaataactc atcatcggcg
    11461 taataatatt ggtaacggaa tcaccgatgc ggtaagcggc ttgaatgact tcgggcgcgt
    11521 agccggccaa catcagcata gggacgaaaa tcggcgcagt taccgcccat tgcgcggagg
    11581 cggagcctat catcagattg ataaaagcac aaattaaaat aaaaccgata aacaacacgc
    11641 tgccgcccaa gccgacttct tttaagaacg tcgccccttt aacggcaata tattgcccaa
    11701 tattcgtcca attaaaaaat gcgacaaact gtgcggcaaa aaagatgatg accaaataaa
    11761 gccccagagt actcatcgat tcggccatcg cattaacgac ttcctgttcg ccgcgcaaac
    11821 ttcgggttac ccggccataa acaatgcccg gcagtgcaaa caacaagaaa ataaaaacaa
    11881 caatcgattt taaaaacggc gaaccggaaa ccaatcctgt ttcaggatga cgcaaaatac
    11941 cgtcggcagg gacgatgctc caagccaata gggcggataa ggcaacaaac accacgccag
    12001 cccaaattaa tcctttatat tccaaaggcg tgatttcatt ggaatgccga atgtcttttt
    12061 cttcttgtga caaatctgat tgataagggc ccaattgcgg ttcgacgatt ttttcagtaa
    12121 caaaataacc aatcaaagca atcacaaacg tactggctac cataaaaaac cagttggctt
    12181 cagggcctac gacgtagtcg ggatggatga tttgcgccgc ctgttgggtg atgcctgcca
    12241 agagcggatc gattgtgcct aagaacagat tggccgaata accgcccgaa acgccggcga
    12301 aagccgcagc cagaccggca agcggatggc ggccgaggga atgaaagatg atggcggaca
    12361 aagggattag gacgacatag cccaattcag aagcggtatt agataaaatc cctgtaaaaa
    12421 caaccataaa agtagtgagt ttgcgtggag attttgtgag caataagcgc attaatgcgg
    12481 aaatcaagcc cgatttttcc gcaatcccca cgcccaataa agaaaccaac accgttccca
    12541 acggcgcgaa accggtgaaa tttttaacgg tatgcgtcag gattttgatc aaaccgtcag
    12601 catcgagcag gctgacaacg tgaatcaaac cgtcatcggc acgtcctttc gcaccaacag
    12661 ggcgcggatc ggggacggat agtccgaaat acgcaccggc ggcagaggca atcagcaata
    12721 acacaatgaa aataataaaa agcgtaaccg ggtgcggcaa catattgccc agccattcga
    12781 ctgtgcgtaa aaatcgtccg tcccgttgcg tatcggtttg actcatctgc ttctccttaa
    12841 aaaaaatata ttgagtgcat taaatttttg aatgtaacat gaagtgtttt gtaagcaaac
    12901 taaaataatc gtatatgtat ataaccgtaa tagattaaaa cagaaaatgc ccacatcgtt
    12961 attctcataa aggcggtaat ccgaaaactt gacgttaaag ccttatcagg aatgactgga
    13021 actcaacaaa gcggattccc gcctgcgcgg gaatgacggg attttcggtt tttgtttgca
    13081 agaatgccgg agaagaacgc agtttacatt ttgaatggta ttgttattaa ttgtaaataa
    13141 atattggaag gtaaatgata tgaaaaatga aattcaaaaa atcatggaca aatataatcc
    13201 atggcatgaa gatgattttg agtcgtatga agacattgcc aaagatgtat cgctgatgac
    13261 agataaaacg ttcattgaac attatttgtt agaagtttat tcagaagaaa acggacattt
    13321 tgaccaagag aatgtccatg caatgataga agaaattaaa aatgcaattt agtgaa
//
LOCUS       DAXHSF010000039        12987 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.039, whole genome shotgun sequence.
ACCESSION   DAXHSF010000039 DAXHSF010000000
VERSION     DAXHSF010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12987
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.039"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            188..1372
                     /gene="ubiM"
                     /locus_tag="ACFM8S_000855"
     CDS             188..1372
                     /gene="ubiM"
                     /locus_tag="ACFM8S_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HFC6313948.1"
                     /translation="MSLHSDILVVGAGPAGLSFAAELAGSGLKVTLIERSPLTVLQNP
                     PYDGREIALTHFSREIMQRLGMWDKIPENEIYPLRDAKVLNGRSDYQLHFPQPTEARG
                     EPADCLGYLISNHNIRRAAYEVVSQLDNVSILTDTVVKEVKTSDNEAQVILENGKILT
                     ARLLLAADSRFSQTRRQLGISSDMHDYSRTMFVCRMKHTLSNQHTAYECFHYGRTIAL
                     LPLEEHLTNTVITVDTDKINSVQNLSPEELAASVKEQLKGRLGDMELVSSIHHYPLVG
                     MIAKRFYGKRSALIGDAAVGMHPVTAHGFNLGLSSADILAKLILEAEQRGQDIGAASL
                     LEKYSSKHMLHAHPLYHGTNMMLKLFTNETAPAKLLRGLVLRAGNNFPPLKKLITKQL
                     TG"
     gene            complement(1643..1798)
                     /gene="rpmG"
                     /locus_tag="ACFM8S_000856"
     CDS             complement(1643..1798)
                     /gene="rpmG"
                     /locus_tag="ACFM8S_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFC6313949.1"
                     /translation="MRDKIKLESSAGTGHFYTTTKNKRTMPGKLEIKKFDPVARKHVV
                     YKETKLK"
     gene            complement(1830..2063)
                     /gene="rpmB"
                     /locus_tag="ACFM8S_000857"
     CDS             complement(1830..2063)
                     /gene="rpmB"
                     /locus_tag="ACFM8S_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFC6313950.1"
                     /translation="MARVCKVTGKRPMSGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWVRLRVSNAALRTIDKVGIDVVLADLRARGEA"
     gene            complement(2313..3839)
                     /gene="farB"
                     /locus_tag="ACFM8S_000858"
     CDS             complement(2313..3839)
                     /gene="farB"
                     /locus_tag="ACFM8S_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     permease subunit FarB"
                     /protein_id="HFC6313951.1"
                     /translation="MDYPPLKGAALAWVTLSLGLAVFMEVLDTTIANVAVPVIAGNLG
                     AATTQGTWVITSFAVANAVSVPLTGFLAKRIGEVKLFTAAAVGFVITSWLCGIAPNLQ
                     SLVVFRILQGFIAGPLIPLSQSLLMASYPPAKRTLALALWAMTVVVAPVLGPILGGWI
                     SGNWHWGWIFFINIPIGIISAWITWKHLKCRETETVKMPTDYVGLTLMVVGIGALQMM
                     LDRGKELDWFASGEIITLGVTALVCLSYFIVWELGEKYPIVDLSLFKDRNFTVGVIAT
                     SLGFMVYMGTLTLLPLVLQTNLGYTSTWAGLAAAPVGILPVFLSPIIGRFGNKADMRL
                     LVTASFLTFAFTFYWRTDFYADMDIGNVIWPQFWQGVGVAMFFLPLTTITLSHMKGGQ
                     IAAAGSLSNFLRVLMGGVGVSVVSTLWERREALHHTRFAEHITPYSATLHETAAHLSQ
                     QGISDIQTLGIINNTITQQGFIIGSNEIFMAGSLLFIIMIPVIWLAKPPFHSSGGGGH
                     "
     gene            complement(3863..5035)
                     /gene="farA"
                     /locus_tag="ACFM8S_000859"
     CDS             complement(3863..5035)
                     /gene="farA"
                     /locus_tag="ACFM8S_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     adaptor subunit FarA"
                     /protein_id="HFC6313952.1"
                     /translation="MATHADETTIQNRQEANSAKAKRKRCLTALTLLFALAAAAAGSA
                     FFLWWQHEEETEDAYVAGRVVQVTPQKGGTVRKVLHDDTDAVKKGDVLAVLDDDNDVL
                     AYERAKNELIQAVRQNRRQNAATSQAGAQVALRRADLARAQDDLRRRSALAESGAVSA
                     EELAHARAAVSQAQAAVKAALAEESSARAALGGQVSLREQPAVQTAIGRLKDAWLNLQ
                     RTQIRAPADGQVAKRSVQVGQQVAAGAPLMAVVPLSDVWVDANFKETQLRHMKIGQPA
                     ELVSDLYGKQIVYRGRVAGFSAGTGSAFSLIPAQNATGNWIKVVQRVPVRIVLNREDV
                     DRHPLRIGLSMTVKVDTSAAGAPVSKTPGAALPEMESTDWSEVDRTVDEILGQSAP"
     gene            complement(5079..5438)
                     /locus_tag="ACFM8S_000860"
     CDS             complement(5079..5438)
                     /locus_tag="ACFM8S_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HFC6313953.1"
                     /translation="MINLELKAINLYDCAFEDFVPEIKDNFCVGIDLDIGTDDSDAAD
                     IFSVRICAPKWILHNCFQNQRVKWGAGMMIMNEFNHSVIKSEIEKILKECSKETWEKS
                     LTYLLRFFSWEFEDYQC"
     regulatory      5653..5697
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            5702..6175
                     /gene="queF"
                     /locus_tag="ACFM8S_000861"
     CDS             5702..6175
                     /gene="queF"
                     /locus_tag="ACFM8S_000861"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046857 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors, with NAD or NADP
                     as acceptor [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HFC6313954.1"
                     /translation="MSRNNEELQGISLLGNQKTQYPTGYAPEILEAFDNKHPDNDYFV
                     KFVCPEFTSLCPMTGQPDFATIYIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNI
                     IMKDLIALMDPKYIEVFGEFTPRGGIAIHPFANYGKAGTEFEALARKRLFEHDAQ"
     gene            6252..6938
                     /locus_tag="ACFM8S_000862"
     CDS             6252..6938
                     /locus_tag="ACFM8S_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFC6313955.1"
                     /translation="MYAFTDAQQQKALFRLVLFHILIIAASNYLVQFPFQIFGIHTTW
                     GAFSFPFIFLATDLTVRIFGSHLARRIIFWVMFPALLLSYIFSVLFHDGGWTGLGALS
                     EFNTFVGRIALASFAAYALGQILDIFVFNKLRRLKAWWIAPTASTVIGNALDTLVFFA
                     VAFYASSDEFMAANWQGIALVDYLFKLTVCGLFFLPAYGVILNLLTKKLTTLQTKQAQ
                     DRPAPSLQNP"
     gene            complement(7287..8579)
                     /locus_tag="ACFM8S_000863"
     CDS             complement(7287..8579)
                     /locus_tag="ACFM8S_000863"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HFC6313956.1"
                     /translation="MAVFPLSAKHRKYALRALAVSIILVSAAYIALTERTERVRPQRV
                     EQNLPPLSWGGSGVQTAYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLR
                     HLRADQSVHVLVGGDGGAREVQFFTDEDGERNLVALEKKGGIWRRSASEADMKVLPTL
                     RSVVVKTSARGSLARAEVPVEIRESLSGIFAGRFSLDGLKEGDAVRLMYDSLYFHGQQ
                     VAAGDILAAEVVKGGTRHQAFYYRSDKEGGGGGNYYDEDGRVLQEKGGFNIEPLVYTR
                     ISSPFGYRMHPILHTWRLHTGIDYAAPQGTPVRASADGVITFKGRKGGYGNAVMIRHA
                     NGVETLYAHLSAFSQAQGNVRGGEVIGFVGSTGRSTGPHLHYEARINGQPVNPVSVAL
                     PTPELTQADKAAFAAQKQKADALLARLRGIPVTVSQSD"
     gene            8656..9624
                     /locus_tag="ACFM8S_000864"
     CDS             8656..9624
                     /locus_tag="ACFM8S_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313957.1"
                     /translation="MKLTLALPSLNLDEDEIRIPLCLPAFNKILQYGSLHRQSCTASA
                     FYARYLWCGRLAERAAQSLNMPSETVALATPVWQKMGMHQANVLTAEYLDVGTDEAER
                     LCRDLSAFYGDIPWRFVPVLPELWLVSLPCAYRWGAKPVLDLGGLLGADDQPDGEDAL
                     EWLRVQTEIQMWLAAHPVNHNRKKRGLPELNGLWLWDGTNGGAQGGTLFADTVWSRFH
                     PDRRALPDNFRAYEETAAHLPDTHHILFMDDLRLTALTGDRERYAAILQQWEERWFAP
                     LYEAVRTGKIKRLDITTDGQHGGTLTFKPADRWKFWRGAKTFDGIW"
     gene            complement(9825..11291)
                     /locus_tag="ACFM8S_000865"
     CDS             complement(9825..11291)
                     /locus_tag="ACFM8S_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC6313958.1"
                     /translation="MVIFYFCGKTFMPARNRWMLLLPLLASAAYAEETPREPDLRSRP
                     EFRLHEAEAKPIDREKVPGQVREKGKVLQIDGETLLKNPELLSRAMYSAVVSNNIAGI
                     RVILPIYLQQAQQDKMLALYAQGILAQADGRVKEAISHYRELIAAQPDAPAVRMRLAA
                     ALFENRQNEAAADQFDRLKAENLPPQLMEQVELYRKALRERDAWKVNGGFSVTREHNI
                     NQAPKRQQYGKWTFPKQVDGTAVNYRLGAEKKWSLKNGWYTTAGGDVSGRVYPGNKKF
                     NDMTAGVSGGIGFADRRKDAGLAVFHERRTYGNDAYSYTNGARLYFNRWQTPKWQTLS
                     SAEWGRLKNTRRARSDNTHLQISNSLVFYRNARQYWMGGLDFYRERNPADRGDNFNRY
                     GLRFAWGQEWGGSGLSSLLRLGAAKRHYEKPGFFSGFKGERRRDKELNTSLSLWHRAL
                     HFKGITPRLTLSHRETRSNDVFNEYEKNRAFVEFNKTF"
     gene            complement(11500..12987)
                     /gene="autA"
                     /locus_tag="ACFM8S_000866"
                     /pseudo
     CDS             complement(11500..12987)
                     /gene="autA"
                     /locus_tag="ACFM8S_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261573.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="autoaggregation autotransporter AutA"
BASE COUNT         3014 a         3823 c         3140 g         3010 t
ORIGIN      
        1 ataccccaca cccgatttga tacacattaa gctgaaagta aaaatccgca tacaccctcc
       61 cctgcatatt tcttcaacag tgggttttga tataatcgcc tatctgttac agatagttca
      121 aacggcattc aaacccttac aaataccgtc tgaaatcatc ggcatccgtc tatgcaaagg
      181 atattttatg agtttacaca gcgacatcct cgtcgtcggc gcaggcccgg cgggtttaag
      241 ttttgcagcc gaacttgccg gcagcggttt gaaagttacc ctgatcgaac gcagcccact
      301 gactgttctg caaaatcctc catatgacgg acgcgaaatc gccctgaccc atttttcccg
      361 cgaaatcatg cagcgtttgg gtatgtggga caaaattccc gaaaacgaaa tctacccttt
      421 gcgcgatgcc aaagtgctga acggacgttc cgactaccag ctccactttc cccaaccgac
      481 tgaagcgcgc ggcgaacctg ccgactgttt gggctatctg atttccaacc acaatatccg
      541 ccgcgccgcc tatgaagtcg tatcacaact cgacaatgtc agcattctga ccgatacggt
      601 tgtcaaagaa gtcaaaacat ctgacaacga agcacaagtc attttggaaa acggcaaaat
      661 cctgactgca cgcctgcttt tggcggcaga cagccgcttc tcgcaaaccc gtcgacaact
      721 cggtatctcc tccgatatgc acgattacag ccgaaccatg ttcgtctgcc gtatgaaaca
      781 taccctttcc aaccaacata ccgcttacga atgcttccat tacggacgca ccatcgcact
      841 gctgccgctg gaagagcacc tgaccaatac cgtcattacc gtcgataccg ataaaatcaa
      901 cagcgttcaa aacctttcgc ccgaagaatt ggcggcaagc gtaaaagaac aactcaaggg
      961 taggcttggc gacatggaac tggtcagcag catccaccat tacccccttg tcggtatgat
     1021 tgccaaacgt ttctacggca aacgcagcgc gctgatcggc gatgcggctg tcggtatgca
     1081 ccccgttacc gcacacggtt tcaacttggg tctgtccagc gcagatattt tggcaaaact
     1141 gatactcgaa gccgagcagc gcggtcagga catcggcgcg gcttccttgt tggaaaaata
     1201 cagcagcaaa catatgctgc acgcccatcc gctttatcac gggaccaata tgatgctgaa
     1261 actgtttacc aacgaaaccg cgccggcaaa actgctgcgc ggtttggtat tgcgtgccgg
     1321 caacaacttc ccgccgctga aaaaactgat taccaaacaa ctgaccggtt gaaaactgcc
     1381 gaaatgccgt ctgaaacgtt tcagacggca tttttgtatt atagtggatt aacaaaaatc
     1441 aggacaaggc gacgaagctg cagacagtac aaatagtacg gaaccgattc acttggtgct
     1501 tcagcacctt agagaatcgt tctctatcct tggcagacgg tttgcagcag attcgcatac
     1561 ggcagatgtt tcaagcggaa aagaaacacg ataaaaataa aaaagcctcc gaacagtcgg
     1621 aggctttgct ttcaaactga aattatttca gtttggtttc tttgtacact acgtgtttgc
     1681 gggcaaccgg atcgaatttt ttaatttcca gtttgccggg catagtgcgt ttgtttttgg
     1741 tagtggtgta gaagtgacca gtacctgcac tggattccag tttgatttta tcgcgcattg
     1801 cagtaatcct taattaaata gtgtttaaat taagcttcgc cgcgagcacg caaatcagcc
     1861 aatacgacat caatgcctac tttgtcgatg gtacgcagtg cagcgttgga aacgcgcagg
     1921 cgaacccagc ggttttcact ttctacccaa aaacgacgtg attgcaagtt gggcaaaaaa
     1981 cggcgtttgg ttttgttgtt ggcgtgcgat acgttgttgc cggacatcgg gcgtttgccg
     2041 gtcactttgc aaactcgtgc cataatcagt ctccaaactt ctaaaatagt aaaacgcgat
     2101 ttataccacg aaaaacagtg ttagttcaag tgttttaaag aaaaacggct gttttgcgcg
     2161 gataatcctt ccgattgtcg catctgcgga acgtttgcag atgcgaaaca gatggggcgc
     2221 gattatagcg gatttcggga actgctgcat tcaaaatgcc gtccgaacat atttccagta
     2281 ttttcagacg gcatttcaag ttttcaaatc cctcaatgtc caccgccgcc actgctgtgg
     2341 aacggcggtt ttgccagcca tatgacgggt atcatgataa tgaataacaa gctgcctgcc
     2401 ataaagattt cattcgatcc gatgatgaag ccctgctggg taatggtatt gttgatgata
     2461 cctagggttt gaatgtcgga aatgccttgt tgggacaaat gcgccgccgt ttcgtgcaat
     2521 gttgcggaat agggcgtgat gtgttcggca aagcgcgtgt ggtgcaacgc ttcgcgccgt
     2581 tcccacaggg tgctgacgac ggatacgccg acaccgccca tcagcacgcg caggaaattc
     2641 gacaggctgc cggcggcggc aatctgcccg cccttcatgt gcgacagtgt gatggtagtc
     2701 agcggcagga agaacatggc aacgcctact ccctgccaaa actgcggcca gatgacgttg
     2761 ccgatatcca tgtcggcata aaaatcggtg cgccaataga aagtgaaggc aaaggtcagg
     2821 aagctggcgg ttacgagcag gcgcatatcg gccttgttgc cgaatctgcc gataatgggg
     2881 gacaggaaaa ctgggaggat gccgacgggc gcggcggcaa gccctgccca cgtggaggta
     2941 tagcccaggt tggtctgcaa cactaacggc agcagggtca gcgtccccat atacaccata
     3001 aaacccaatg acgtggcaat gacgccgacg gtaaaattcc gatctttaaa cagcgataaa
     3061 tcgacaatcg gatatttttc gcccaattcc caaacaataa aatacgacaa gcacaccaat
     3121 gcggttacgc ccaaggtaat gatttctccc gaagcgaacc agtcgagttc cttacccctg
     3181 tccaacatca tctgtaacgc gccgataccg actaccatca atgtaagccc gacatagtcg
     3241 gtcggcattt taacggtttc cgtttcccga catttcaaat gtttccatgt aatccatgcc
     3301 gatatgatac cgatagggat attaatgaag aaaatccaac cccaatgcca gtttccggaa
     3361 atccagccgc cgagtatcgg cccgagaaca ggggcgacaa cgacggtcat tgcccacaat
     3421 gccagtgcca gcgtccgttt tgcgggcgga taggatgcca ttaacaggct ttgcgacagg
     3481 ggaatcagcg gcccggcgat aaagccctgc aagatgcgga aaacaaccag cgactgaagg
     3541 ttgggggcaa taccgcacag ccacgatgtg atgacgaaac cgacagcggc ggcggtaaac
     3601 aatttgacct cgccgatacg ttttgccaaa aagcccgtta acggcacgga aacggcgttt
     3661 gccacagcaa aagaagtaat gacccacgtc ccctgcgtgg ttgccgcacc gaggttgccg
     3721 gcgatgacgg gaacggcgac attggcgata gtcgtatcta aaacttccat aaatacggca
     3781 agccccaaag acagcgtaac ccacgccaat gccgcaccct taagcggtgg ataatccatt
     3841 gtgtcctccg tttcagacgg catcagggcg cggattgccc gaggatttca tcgaccgtcc
     3901 gatcgacttc cgaccagtcg gtactttcca tttccggcaa tgccgcaccc ggcgtttttg
     3961 aaacaggcgc gcctgcggcg gaagtatcca ctttaaccgt catcgacaaa ccgatacgca
     4021 acggatgcct gtccacatct tcgcggttca gcacgatacg gacggggacg cgctgcacca
     4081 ctttaatcca gttgcccgtt gcgttttgcg ccggaatcag cgaaaacgcg ctgcccgtac
     4141 ctgccgaaaa acctgccacc ctgccgcgat aaacaatttg tttgccgtac aaatcggaca
     4201 ccagctcggc aggctgtccg attttcatat gccgcaactg cgtctcttta aaattagcat
     4261 ccacccacac atccgacagc ggcaccaccg ccatcagcgg cgcgcctgcc gccacctgct
     4321 gcccgacctg caccgaacgc ttcgccacct gaccgtccgc cggcgcgcgg atttgcgtcc
     4381 gctgaaggtt caaccacgca tctttcaacc tgccgattgc cgtctgaacc gccggctgtt
     4441 cgcgcaaaga aacctgaccg cccaaagccg cacgcgccga agattcttcc gccaaagccg
     4501 ccttaactgc cgcctgagcc tgagacactg ccgcacgggc atgtgccagc tcttcggcgg
     4561 acaccgcgcc cgattccgcc aaagcagacc ggcggcgcaa atcatcctgc gcgcgtgcca
     4621 aatccgcccg gcgcaaggca acctgcgccc ccgcctgcga agtggcggca ttttgccggc
     4681 ggttttgccg caccgcctga atcagctcgt tttttgcccg ctcgtaagcc agcacatcat
     4741 tatcgtcgtc caataccgcc agcacgtcgc cttttttcac ggcatccgta tcgtcgtgca
     4801 aaaccttccg caccgtaccg cccttttgcg gcgtaacctg aaccacgcgt ccggcaacat
     4861 aagcgtcttc cgtttcctct tcgtgctgcc accataaaaa aaacgccgac ccggcggctg
     4921 cggcggcaag cgcgaacagc agcgtcaatg ccgtcaggca gcgtttgcgt ttggcttttg
     4981 cgctattggc ttcttgtctg ttttgaattg tcgtttcatc tgcatgtgtt gccattattc
     5041 caattccaaa tacttctaat actatgtttt tgatttaatt agcactgata atcttcaaat
     5101 tcccacgaaa aaaaacgaag taaataagtc aatgactttt cccaagtttc ttttgaacat
     5161 tctttaagaa ttttctcaat ttccgattta ataacagaat gattaaattc attcataatc
     5221 atcatacccg ctccccattt aaccctttga ttttggaaac aattatgcaa aatccattta
     5281 ggagcgcata tgcgaacaga aaatatatct gcagcatcac tatcatcagt tcctatgtct
     5341 aaatcaattc ccacacaaaa attgtctttg atttcgggaa cgaaatcttc aaaggcacaa
     5401 tcgtaaagat tgatggcttt caattctagg ttaatcattt tatattcaaa ataaataaaa
     5461 ccaattaaag attggttagg attctatacc ctggcaataa ctcaatcaag ttaatgattt
     5521 agaatgaata ttcaaataaa ctgtatcagc ccggcctcac agaactattt atatccacac
     5581 atcaccgagt aaagcaatta cccatactaa ccgaagaaaa ctacacaaat accgccactt
     5641 atattacaat cgccgcccgt ggttcgaaaa cctcccacat taaaaaacta aggaaaccct
     5701 catgtcccgc aacaacgaag agctgcaagg catctctctt ttgggcaatc aaaaaaccca
     5761 atatccgacc ggctacgcgc ctgaaattct cgaagcattc gacaacaaac atcccgacaa
     5821 cgactatttc gtcaaattcg tctgtcccga gttcaccagc ctctgcccca tgaccgggca
     5881 gcccgacttc gccaccatct acatccgcta cattccgcac atcaaaatgg tggaaagcaa
     5941 atccctgaaa ctctacctct tcagcttccg caaccacggc gattttcatg aagactgcgt
     6001 caacatcatc atgaaagacc tcattgccct gatggatccg aaatacatcg aagtattcgg
     6061 cgagttcaca ccgcgcggcg gcatcgccat tcatcctttc gccaattacg gcaaagcagg
     6121 caccgagttt gaagcattgg cgcgtaaacg cctgtttgag cacgacgcac aataaaccat
     6181 ccttcccata tttcagacgg cattctttcg atacggtatg ccgtctgaac gcctttccga
     6241 aaggtttttt tatgtacgca tttaccgacg cacagcaaca gaaggcactc ttccggctgg
     6301 tgctttttca tatccttatc atcgccgcca gcaactatct ggtgcagttc cccttccaaa
     6361 ttttcggcat ccacaccact tggggcgcat tttcctttcc cttcatcttc cttgccaccg
     6421 acctgaccgt ccgcattttc ggttcgcact tggcacggcg gattatcttt tgggtgatgt
     6481 tccccgccct tttgctttcc tatatctttt ccgttttgtt ccacgacggc ggttggacgg
     6541 gcttgggcgc actgtccgaa ttcaacacct ttgtcggacg catcgcgctg gcaagttttg
     6601 ccgcctacgc gctcggacaa atcctcgata tttttgtatt caacaaatta cgccgtctga
     6661 aagcgtggtg gattgccccg accgcatcaa ccgtcatcgg caacgccttg gatacgctgg
     6721 tatttttcgc cgttgccttc tacgcaagca gcgatgaatt tatggcggca aactggcagg
     6781 gcatcgcctt ggtcgattac ctgttcaaac tcaccgtctg cggtctgttt ttcctgcccg
     6841 cctacggcgt gattctgaat ctgctgacga aaaaactgac gaccctgcaa accaaacagg
     6901 cgcaagaccg ccccgcgccc tcgctgcaaa atccgtaaca cagccctgct gggaatcggg
     6961 acggttgggt tttcttttat ttgcaaacac tgccggaacg gataaatccg gattcccgcc
     7021 acttcgtcat tcccgcgaaa gcgggaatcc ggtgcgttgg gtttcagtca tttccgataa
     7081 actcctgccg cgttgtattt ctagattccc gctttcgcgg gaatgacggc ggatgggttt
     7141 ccgtgccgtg gtgtccggat tcccgcccgt acgggaatga cggcagtgct tccttggctt
     7201 aattttattt taatcaactg caaatgccgt ctgaaaacct gtttgcagat tttcagacgg
     7261 cattgttttc gtcgccggtt caaacttcaa tccgattgcg acacggtaac cggtatgccg
     7321 cgcaagcgcg caagcagcgc gtccgccttc tgtttctgcg cggcaaacgc cgccttgtcc
     7381 gcctgcgtca attcgggtgt cggcaatgcg actgaaacag gattgacggg ctgcccgttg
     7441 atgcgtgcct cgtaatgcag gtgcggcccg gtcgaacgcc ctgtcgaacc gacaaaaccg
     7501 atgacctcgc cgccgcgcac attgccttgt gcctgcgaaa acgcgctcaa gtgcgcgtac
     7561 agcgtttcca caccgttggc gtggcgtatc atcaccgcgt tgccgtatcc gcccttccgg
     7621 cctttaaagg taatcacgcc gtcggcggaa gccctgaccg gcgttccctg cggtgcggca
     7681 taatcgatgc ccgtgtgcag ccgccaagtg tgcaggatgg ggtgcatacg gtagccgaac
     7741 ggcgaagaaa tgcgcgtata gaccagcggc tcgatgttga agccgccttt ttcctgcaac
     7801 accctgccgt cttcatcata ataattgccg ccccctccgc cttccttgtc cgaacggtaa
     7861 tagaacgcct gatgccttgt gccgccctta acgacttcag ccgccaaaat atcgcccgcc
     7921 gccacctgct gcccgtggaa atacaggctg tcgtacatca ggcgcacggc atcgccttcc
     7981 ttcaaaccgt caaggctgaa gcggccggcg aaaatcccgc ttaaggattc gcggatttcg
     8041 acgggcactt ccgcccgcgc cagcgaaccg cgcgccgacg ttttgaccac gaccgaacgc
     8101 agcgtcggca aaaccttcat atccgcctca gaagccgacc gccgccatat gccgcctttc
     8161 ttttccaaag cgaccagatt gcgctcgccg tcttcgtcgg taaaaaactg cacttcgcgc
     8221 gcgccgccgt cgccgccgac caaaacatga accgactggt cggcacgcaa atgccgcaaa
     8281 tcggcttcgc cgccatattt ttccgtgatt cgggcaatct cgtcccgcgc catacccgaa
     8341 cgcgccagca cgtccgccag cgagtcgccc ggctgcaccg cctcctgcac ccaatatgcc
     8401 gtctgaacgc cgctgccgcc ccaagacagc ggcggcagat tttgttccac gcgctgcggt
     8461 ctgacgcgtt ccgtcctctc tgtcaaagca atgtatgccg ccgacaccaa aataatcgaa
     8521 acggcaagcg cgcgcagcgc gtatttccga tgttttgccg aaagtgggaa gacagccata
     8581 ttgttccttt ttgacggcgt ttgcgccgaa tcctctataa tatccgcccg atccgcattt
     8641 gtcagacatc aaacgatgaa actcaccctc gccctcccct cgctcaattt ggatgaagac
     8701 gaaatccgaa tacctctatg cctgcccgcc ttcaacaaaa tcctgcaata cggctcgctg
     8761 caccgccaaa gctgcacggc ttcggcattt tacgcgcgtt atttgtggtg cggcagactg
     8821 gcggaacgcg cggcgcaaag cctgaatatg ccgtctgaaa ccgtcgcgct cgccacgccc
     8881 gtctggcaaa agatggggat gcatcaggca aatgtgctga cggcggaata tttggatgtc
     8941 ggtacggatg aagcggaacg gctctgccgc gatctgtcgg cgttttacgg cgatattccg
     9001 tggcgtttcg ttcccgtcct gcccgaatta tggctggttt cccttccttg cgcgtaccgc
     9061 tggggcgcga agcccgtttt ggacttgggc ggattgttgg gcgcggacga tcagccggac
     9121 ggagaagatg cgttggaatg gctgcgcgtc caaaccgaaa tccaaatgtg gctggcggcg
     9181 caccccgtca accataaccg caaaaaacgc gggctgcccg agttgaacgg cttatggctt
     9241 tgggacggca caaacggcgg cgcgcaaggc ggcacgcttt tcgccgatac ggtttggagc
     9301 cgtttccacc ccgaccgccg cgcattgccc gacaacttcc gcgcctatga agaaacggcg
     9361 gcacacctgc ctgacacgca ccatatcctg tttatggacg atttgcgcct gaccgccctg
     9421 acaggggaca gggagcggta tgcggcaata ttgcagcagt gggaagaacg ctggttcgca
     9481 ccgctttacg aagccgtccg taccggcaaa atcaaacggc tggacatcac cacggacgga
     9541 caacacggcg gtacgctgac cttcaaaccc gccgacaggt ggaaattttg gcgcggcgcg
     9601 aaaacctttg acggaatctg gtgaacccgc ctgccgcaac cgcgacaatg ccgtctgaag
     9661 cccgaatcgg gtttcagacg gcattttacc tcgcatcgcc gttatgccgg aaaaaccgga
     9721 atgcgtttcc gcacacgcgg cggcacgcaa agcaccgccc gaaagccgcc gatgcccgcc
     9781 gtgataccgc cgccggcagg gtttcctccg aaaaggaaca gcaatcagaa cgttttatta
     9841 aactcgacaa acgcccgatt tttctcgtat tcgttgaaca catcgttact ccgcgtttcg
     9901 cggtgcgaca acgtcaggcg cggcgtgatg cctttgaaat gcaatgcccg gtgccaaagg
     9961 ctcaaggatg tgttcaattc tttatccctg cgcctttccc ctttaaaacc gctgaaaaag
    10021 ccgggttttt cataatgccg tttcgccgcg ccgaggcgca acagcgaaga caggccgctg
    10081 ccgccccatt cctgccccca ggcaaagcgc aggccgtaac ggttgaaatt gtcgccccgg
    10141 tcggcggggt tgcgctcgcg gtaaaaatcc aaaccgccca tccaatattg gcgcgcattc
    10201 cggtaaaaca ccagcgaatt ggaaatttgc aaatgggtat tgtcggaacg cgcccggcgc
    10261 gtattcttca aacgccccca ctccgccgaa gacaacgttt gccatttcgg ggtttgccaa
    10321 cggttgaaat aaaggcgtgc gccgttggtg taagaataag cgtcgttgcc gtaggtgcgg
    10381 cgttcgtgga acactgccag cccggcatct ttgcgccggt cggcaaaacc gatgccgccg
    10441 gaaacgcctg ccgtcatatc gttgaatttc ttattccccg gataaaccct gccggacacg
    10501 tcgccgcccg ccgtcgtgta ccagccgttt ttcagcgacc attttttctc cgcgccgagc
    10561 cggtaattga ccgccgtgcc gtccacctgt ttcgggaaag tccatttgcc gtactgctgc
    10621 cgtttcgggg cttggttgat attgtgttcg cgggtgacgc tgaagccgcc atttaccttc
    10681 cacgcatcgc gttcgcgcaa tgccttgcgg tacagctcga cctgctccat cagctgcggc
    10741 ggcaggtttt ccgccttcag gcggtcgaac tggtctgccg ccgcctcgtt ctgcctgttt
    10801 tcaaacaatg ccgccgccaa acgcatacgg acggcgggcg cgtcgggttg ggcggcaatc
    10861 aattcccggt aatgggaaat cgcctccttc accctaccgt ctgcctgcgc caaaatccct
    10921 tgtgcataaa gtgccaacat cttatcctgc tgcgcctgtt gtaggtaaat tggcaaaata
    10981 acgcggatac cggcaatatt gtttgaaacc actgcggaat acatcgcgcg ggacagtaat
    11041 tcgggatttt tcagcagggt ttcgccgtca atctgcaaaa cttttccttt ttcccgcacc
    11101 tgccccggta ccttctccct gtcgatcggt ttggcctccg cttcatgaag cctgaactcg
    11161 ggacggcttc tcaaatccgg ttcgcgcggt gtttcttcgg catatgccgc gcttgccaat
    11221 aaaggcagca gcagcatcca tctgtttcgt gcaggcataa atgtcttccc acaaaaataa
    11281 aaaataacca ttatcaaccg gcggattgta tcagtttttt atgccgctgt tccaaaccct
    11341 gccgcccgat gccgcaacaa tgccgtctga agcccgaacc gggtctcagg tggcaaaaaa
    11401 ccggcagttt cccgccggtt tcatcattat gcgctgccct tcgggtcggc agggttcgga
    11461 ttggtgtgcc gcctgtgcgt caaaacattt cccgacgcat caaaacagcc atttgagcga
    11521 caatgcggct tctttgttgc cgtccgtcct tttgccgtat ccgatgcgtg cggacatatg
    11581 gcctttccaa ccggcttcaa tgccgaaccg ccccgagtgc cgtcctgcct gccagcgtct
    11641 gtttttcgcc gtccatttcc acgccgaaag attttgacct gtgcaaaaca ttaaaagcgg
    11701 cgaaaggctg aagattgaca ccgttacgca aagcaaaacg ggtttttgcc cgaatgccgg
    11761 cgcggctttg ccactgaccg ctgccgagca gtccgacccc cgtcccctcg ctgtcggtaa
    11821 agccgccgtt tacgcccaag taggtaaact gcgcctgcgg ttgcaggtaa aaccgcacat
    11881 tattgccttt tccgacaacg ccttccgcca caagcgcgtt gtagccgcct tcgacagaag
    11941 ccgtccaacc tttggttttg tagcgttccg cacggttttc atcattgatg cggtgtttga
    12001 aacgttggta ttgcaaccag ccgtccaaat acgcacccgt ttgtttatcg cgcaactgat
    12061 gccacgcagc ataaacaccc ccgccataac catgcaaata actgcctgcc gcaccgcctt
    12121 tgccgttggc tgatgcgtgc tggccggccc tgccgcccat cacgccgatt gccagccggc
    12181 tgccttcatt ttgccgtaca aacacctcgc cgccgatttg cacgcctttg ctccgcccgt
    12241 ccgcagccgc gccgccccgt atattttgat gcgaccggcc gccgatgaag cgcagccaca
    12301 atttttgacg ggacggatcg gcggcataca cgtcgtcgcc cctgtcggcg gcacgcagcc
    12361 caaacaaagt atttgccgca taagcctgtt gggcatataa aaccgcttcc gcctcaggga
    12421 tagccaaacg ctgcacaccc aagcaaaatc cgccttcgca ttgcttgagc aataattcgt
    12481 acagtccctg ccggtaattt tgtttaagcg caaacgaatt agggtctgcc ttttccgccg
    12541 caatcagttt gcgcccgtcg agcgttttca gattttccgg ccggcgttcc aaaaccacgc
    12601 cggattttcc gctaacgttt tgttcaaagg tcagggcggg ttcgtccgac tcccgcacgt
    12661 cgtagccgaa caggaaatcc gcgccgccct gatacgcttt atggacggtc agtttttcat
    12721 ctttcgtgtt taggcggatc cgggcattat cggaagtttc cagcatatgg atgtcggaat
    12781 ggcggcgcgg ttcccaaagg gaatctttca gcgtcatctt ggtcagttcc aacacctgtc
    12841 cggcgacgtg ggatttgttt tcgattttga cgtgtaattt tttattgaag atgttgttga
    12901 gactagtctg tgatgtatta ctatgaggag tgagtacgga atcttctacc aatttattaa
    12961 ttaaatcggg gtttttctgt ttgagta
//
LOCUS       DAXHSF010000040        12863 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.040, whole genome shotgun sequence.
ACCESSION   DAXHSF010000040 DAXHSF010000000
VERSION     DAXHSF010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12863
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.040"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(39..764)
                     /gene="aat"
                     /locus_tag="ACFM8S_000867"
     CDS             complement(39..764)
                     /gene="aat"
                     /locus_tag="ACFM8S_000867"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFC6313959.1"
                     /translation="MRIPLLAPDNYAFPDPTYALARCDGLVGVSGDLDAGRLLEAYRN
                     GVFPWFSRDGWFFWYAVGPRAVIVPERLHVPRSLAKTLRNGSYRVAVNGCFAEVVAHC
                     AAAARPNQDGTWIAPEFQTAYLKLHEMGHAHSFECHYPDESGETRLAGGFYGVQIGRV
                     FYGESMFALQPDASKIAFACAVPFLADLGVELIDCQQDTEHMRRFGSELLPFADFAER
                     LRMLNAVPLKEEIGRREVACRGL"
     gene            complement(834..1268)
                     /gene="fur"
                     /locus_tag="ACFM8S_000868"
     CDS             complement(834..1268)
                     /gene="fur"
                     /locus_tag="ACFM8S_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFC6313960.1"
                     /translation="MEKFNNIAQLKDSGLKVTGPRLKILDLFETHSEEHLSAEDVYRI
                     LLEEGVEIGVATIYRVLTQFEQAGILQRHHFETGKAVYELDKGDHHDHIVCVKCGEVT
                     EFHNPEIEALQDKIAEENGYRIVDHALYMYGVCSDCQAKGKR"
     gene            1487..1864
                     /locus_tag="ACFM8S_000869"
     CDS             1487..1864
                     /locus_tag="ACFM8S_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFC6313961.1"
                     /translation="MNKTLILALSALLGLAACSAERASLFPSYKLKVIQGNEIDPRAA
                     AALRLGMTKDQVLLLLGSPLLRDAFHTDRWDYTFNTSRNGIIKERSNLTVYFENGVLV
                     RTEGDALQNAAEALKDRQNTDKP"
     gene            1874..2683
                     /gene="dapB"
                     /locus_tag="ACFM8S_000870"
     CDS             1874..2683
                     /gene="dapB"
                     /locus_tag="ACFM8S_000870"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFC6313962.1"
                     /translation="MTPLKIAIAGANGRMGRVLVEAVNNHPDTVLSGALEHSGSEALG
                     LDAGYAVGLKTGIAISDDVDAVLAQSDVLIDFTRPEPTLKHLQKCVEKQVNIIIGTTG
                     FDDAGKAAIRAAAEKTGIVFAANFSVGVNLTFHILDTVALVLNEGYDIEIIEGHHRHK
                     VDAPSGTALRMGEVIAGALGRDLKQCAVYGREGHTGPRDPSTIGFATVRAGDIVGDHT
                     ALFATDGERVEITHKASSRMTFAAGAVRAAVWVNGKTGLYDMQDVLGLNNR"
     gene            complement(2680..2925)
                     /locus_tag="ACFM8S_000871"
     CDS             complement(2680..2925)
                     /locus_tag="ACFM8S_000871"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313963.1"
                     /translation="MPRKQLYRISFSVETPPLGHLSFGAVESDFIWEGRNPFRIRATH
                     RATLYVSSCVLKHMPIRSLSAKCRLNNIFSDGILYGG"
     gene            complement(2943..3353)
                     /locus_tag="ACFM8S_000872"
     CDS             complement(2943..3353)
                     /locus_tag="ACFM8S_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313964.1"
                     /translation="MTTYTIPKKDYQFLYIYEGTLLNYTLKNDEFHIIVNCVDYPDLP
                     QEIPIPNYSDWVKIKFKQFSYLKFIYGYATKNQDKNIKNVLELGELKQDDEIWDYGGA
                     LEVIGGRYDLPTGFSIYIVCREIELEFLDQESFN"
     gene            complement(3355..3600)
                     /locus_tag="ACFM8S_000873"
     CDS             complement(3355..3600)
                     /locus_tag="ACFM8S_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313965.1"
                     /translation="MDYLLEAARITKEGGEIVINGTSNNKYLRGIPNEGELARVGLRL
                     KYNGPLKEEFKQLKFHKSSRTNLDSKNLKLIVFEKVK"
     gene            complement(3608..4780)
                     /gene="lpxB"
                     /locus_tag="ACFM8S_000874"
     CDS             complement(3608..4780)
                     /gene="lpxB"
                     /locus_tag="ACFM8S_000874"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFC6313966.1"
                     /translation="MWGQTNMADKKSPLIAVSVGEASGDLLGAHLIRAIRKRCPQARF
                     VGIGGELMKAEGFESLYDQERLAVRGFVEVVRRLPEILRIRRGLVRDLLSLKPDVFVG
                     IDAPDFNLGVAERLKRSGIPTVHYVSPSVWAWRRERVGKIVHQVNRVLCLFPMEPQLY
                     LDAGGRAEFVGHPMAQLMPLEDDRETARQTLGVDAGIPVFALLPGSRVSEIDYMAPVF
                     FQTALLLLERYPAACFLLPAATEATKRRLAEVLQRPEFAGLPLTVIDRQSETVCRAAD
                     AVLVTSGTATLEVALCKRPMVISYKISPLTYAYVKRKIKVPHVGLPNILLGKEAVPEL
                     LQSEAKPEKLAAALADWYEHPDKVAALQQDFRALHLLLKKDTADLAARAVLEEAGC"
     gene            complement(4828..5820)
                     /locus_tag="ACFM8S_000875"
     CDS             complement(4828..5820)
                     /locus_tag="ACFM8S_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFC6313967.1"
                     /translation="MKTHEISKDSVSLIGVAEHEAGQRLDNYLIKILKGVPKSHIHRI
                     IRAGEVRLNKKRCKPDSRIAEGDTVRIPPVRVAEKEMPSERRAAVPARAFEAVYEDDA
                     LLVIDKPSGVAVHGGSGVSFGVIEQLRRARPEARYLELVHRLDKDTSGLLMVAKKRSA
                     LVKLHEAIRNDHPKKIYLALGVGKLPDDNFHVRLPLSKYTGAQGEKMVRVSADGQSAH
                     TVFRVLSRFSDGILHGVGLSHLTLVRATLKTGRTHQIRVHLQSQGCPIAGDERYGDYQ
                     ANRRLQKLGLKRMFLHASELHLNHPLTGEPLVLKVGLPPDLAQFAVMLENGTKM"
     gene            6314..9073
                     /locus_tag="ACFM8S_000876"
     CDS             6314..9073
                     /locus_tag="ACFM8S_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HFC6313968.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQNLLDLDIETLGKEQRKGNIYKG
                     IITRIEPSLEACFVDYGTDRHGFLPFKEVSRSYFRDYEGGRARIQDVLKEGMEVIVQV
                     EKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERQELKAAMAELDIPN
                     GMSIIARTAGIGRSAEELEWDLNYLKQLWQAIEEAGKAHHDPYLLFMESSLLIRAIRD
                     YFRPDIGEILVDNQEVYDQVSEFMSYVMPGNIGRLKLYEDHTPLFSRFQIEHQIESAF
                     SRSVSLPSGGAIVIDHTEALVSIDVNSARATRGADIEDTAFKTNMEAAEEVARQMRLR
                     DLGGLVVIDFIDMENPKHQRDVENVLRDALKKDRARVQMGKLSRFGLLELSRQRLKPA
                     LGESSHVACPRCAGTGVIRGIESTALHVLRMVQEEAMKDNTGEVRAQVPVDVATFLLN
                     EKRAELFAMEERLDVNVVLIPNIHLENPHYEINRIRTDDVEEDGEPSYKRVAEPEEDE
                     SAKPFGGEKAKAARPEPAVKGVRHTSPAPTAAPEKKTSWWDSFKAWLKRIFGGSKTQA
                     APAAETSEKRSTANRSGSRANNRRQNPRRSKREGSKVEVREVAGKTAGQEARADKAET
                     RNNGNRRRNERGDRAAGRADEAEIQDRNIQPAATVADAAPSETEVQTGKRRRNGSRSE
                     RGQTAPETAAVAETTVQTAENTPSEPHTAEDKGSKPKSERNRRERDSRDAKERRERNN
                     QRDRRQNGKKRNIPSAAKIEQYLNIHDTADKVRSAAAHVFGETDANAPITVSIADPVA
                     ERDLPTASPAVSNGDAPVYDAAEKIRRVTAAILPEGVAPKAEAQEMPSETATFTAAAE
                     QARETAQTGGLVLIETDPAALKAWAAQPEVQTGRGLRRSEQPKPSEVATVPAEEMIQV
                     ETRQG"
     gene            complement(10098..11087)
                     /gene="pdxA"
                     /locus_tag="ACFM8S_000877"
     CDS             complement(10098..11087)
                     /gene="pdxA"
                     /locus_tag="ACFM8S_000877"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFC6313969.1"
                     /translation="MKQPVFAVTSGEPAGIGPDICLDLAFARLPCRCAVLGDKNLLRA
                     RAEALGKSVVLRDFDPESGGAAYGELEVLHIPAAEAVEAGRLNPANAAYVLQLLDTAL
                     AGISDGIFDGIVTAPLHKGIINDARASTGFFSGHTEYLAEKSGTGQVVMMLAGKGLRV
                     ALVTTHLPLKDVAAAITQPLIESVARILHHDLKHKFCIKNPKILVAGLNPHAGEGGHL
                     GHEETDTIIPALENLRRERINLAGPYPADTLFQPFMLEGADAVLAMYHDQGLPVLKYH
                     SFGQGVNITLGLPFIRTSVDHGTALDLAATGRADSGSLITAVETAVEMARGSL"
     gene            11247..12674
                     /locus_tag="ACFM8S_000878"
     CDS             11247..12674
                     /locus_tag="ACFM8S_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC6313970.1"
                     /translation="MNENFTEWLHGWVGAINDPMWSYLVYMLLGTGLFFTVTTGFVQF
                     RLFGRSIKEMLGGRKQGDDPHGITPFQAFVTGLASRVGVGNIAGVAIAIKVGGPGAVF
                     WMWVTALIGMSSAFVESSLAQLFKVRDYDNHHFRGGPAYYITQGLGQKWLGVLFALSL
                     IFCFGFVFEAVQTNTIADTVKAAWGWEPHYVGVALVILTAPIIFGGIRRISKAAEIVV
                     PLMAVLYLFIALFIILTNIPMIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKR
                     GLYSNEAGMGSAPNAAAAAEVKHPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYG
                     DLSGAALTQAAIVSQVGQWGAGFLAVILFMFAFSTVIGNYAYAESNVQFIKSHWLITA
                     VFRMLVLAWVYFGAVANVPLVWDMADMAMGIMAWINLVAILLLSPLAFMLLRDYTAKL
                     KMGKDPEFKLSEHPGLKRRIKSDVW"
BASE COUNT         3078 a         3972 c         3165 g         2648 t
ORIGIN      
        1 tccaccatat ttgaacctga ccgaagcata agccgccatc aaagccccct gcacgccact
       61 tcgcgccgcc cgatttcctc tttcaacggc acggcgttca acatccgcag acgttcggca
      121 aaatccgcaa acggcagcaa ctccgaaccg aaacggcgca tatgttccgt atcctgctgg
      181 cagtctatca gttccacgcc caaatccgcc aaaaacggca cggcgcaggc aaacgcgatt
      241 ttcgacgcat ccggttgcaa cgcaaacatt gattcgccat aaaacaccct gccgatttgc
      301 acgccgtaaa agccgcccgc caacctcgtt tcaccgcttt catcgggata atggcactcg
      361 aacgaatgcg cgtgtcccat ttcgtgcagc ttcaaatatg ccgtctgaaa ctcgggcgca
      421 atccaagttc cgtcctgatt cgggcgcgcc gctgccgcac aatgcgcgac cacttccgca
      481 aaacagccgt tgaccgcaac ccgatagctg ccgttgcgca gcgttttcgc cagcgagcgc
      541 ggaacgtgca gacgttcggg aacaatcacc gcacgcgggc cgaccgcata ccaaaaaaac
      601 caaccgtccc gagaaaacca cggaaacacg ccgttccgat acgcctcgag cagccgcccc
      661 gcatccaaat cgccgctcac gccgaccagc ccgtcgcacc gggccaaagc ataggtagga
      721 tcgggaaagg cataattgtc aggggcaagc agcggaatac gcatggcagt tctccaaaaa
      781 cacagacggc atccaaaatc atgaatgccg tctgaacaac aaaccgtccg gatttaacgt
      841 ttgcccttgg cctgacagtc gctgcacacg ccgtacatat aaagcgcgtg atcgacgatg
      901 cggtagccgt tttcctcagc gattttgtct tgcagggctt cgatttcggg attgtggaat
      961 tccgttacct cgccgcactt cacgcagacg atgtggtcat ggtggtcgcc tttgtccaac
     1021 tcataaaccg ccttgcccgt ttcaaaatga tggcgttgca gaatacccgc ctgctcaaac
     1081 tgggtcagca cacggtaaat cgtcgccaca ccgatttcca caccctcttc caacaaaatg
     1141 cggtacacat cttccgcact caaatgctct tccgaatgcg tctcgaacaa atccaaaatc
     1201 ttcaaacgcg ggccggtaac cttcagaccg ctgtctttca gttgtgcaat attgttgaat
     1261 ttttccataa tattcaatac ccctgtaaaa caatagacgt tataatacgc aatttcggcc
     1321 tgtttgccca ctatcgcacc atagcagttt gcaatagtaa aaccgcaccg gcggccgatg
     1381 cgcggacagg gcggataccg cttaatcgta tgattatcgt tcgcttttat aaaatattca
     1441 agcagtgtta cactacccat cccgatttgc acagaaaggc atttccgtga acaaaaccct
     1501 catcctcgcc ctttccgccc tccttggcct tgccgcgtgc agtgccgaac gcgcctcgct
     1561 gttcccctca tacaagctca aagtcataca gggcaacgaa atcgaccccc gcgccgccgc
     1621 cgcactccgc ctcggtatga ccaaagacca agtcctgctc ctgctcggca gccccctgtt
     1681 gcgcgacgcg ttccacaccg accgctggga ctataccttc aacacctccc gcaacggcat
     1741 catcaaagaa cgcagcaatc tgaccgtcta ttttgaaaac ggcgtactcg tccgcaccga
     1801 aggcgacgcc ctgcaaaacg ctgccgaagc cctcaaagac cgccaaaaca cagacaaacc
     1861 ataaggaaca cacatgacac cgctcaaaat cgccatcgcc ggcgcaaacg gccgtatggg
     1921 acgcgtattg gttgaagccg tcaacaacca tcccgacacc gtcctttccg gcgcgctcga
     1981 acactcaggc tcagaagccc tcggactgga cgcaggctac gccgtcggac tcaaaaccgg
     2041 catcgccatt tcagacgacg ttgacgccgt tctcgcacaa agcgacgtac tcatcgactt
     2101 cacccgcccc gagcccaccc tcaaacacct gcaaaaatgc gttgaaaaac aagtcaacat
     2161 catcatcggc acaacaggct tcgacgatgc gggcaaagcc gccatccgcg ctgccgccga
     2221 aaaaacaggc atcgttttcg ccgccaactt cagcgtcggc gtcaacctca ccttccacat
     2281 cctcgacacc gtcgccctcg tactcaacga aggctacgac atcgaaatca ttgaaggtca
     2341 ccaccgccac aaagtcgacg cacctagcgg caccgcgtta cgcatgggcg aagtcatcgc
     2401 cggcgcgctc ggcagagacc tcaaacaatg cgccgtttac ggccgcgaag gccacaccgg
     2461 cccgcgcgat ccgtccacca tcggctttgc caccgtccgc gcgggcgaca tcgtcggcga
     2521 ccacaccgcc ctcttcgcca ccgacggcga gcgcgtggaa atcacccaca aggcaagcag
     2581 ccgcatgacc tttgccgccg gcgccgtccg cgccgcagtt tgggtaaacg gtaaaacggg
     2641 tttgtacgat atgcaggacg tactcgggct gaacaaccgt taacccccat acaaaatgcc
     2701 gtctgaaaag atattgttca gacggcattt tgccgacagg ctccgtatcg gcatatgttt
     2761 caacacacag gacgacacat aaagcgtcgc cctatgtgtt gccctgattc ggaaggggtt
     2821 acgcccctcc caaataaagt ctgattctac cgccccgaag gacagatgtc cgagtggcgg
     2881 ggtttcaacc gaaaaggaaa tacgataaag ttgcttgcgc ggcatgacaa gctacggctc
     2941 gtttaattaa aactctcctg atctaaaaat tctaactcta tttcccggca aactatatat
     3001 atgctaaaac cggtcggaag atcataccta ccgcctatta cttccagcgc acctccataa
     3061 tcccagattt catcatcctg ctttaattct ccaagttcca atacattttt gatattttta
     3121 tcttggttct tcgtggcgta tccatagata aatttcagat agctgaattg cttgaattta
     3181 atttttaccc agtctgaata attaggtata gggatttctt gaggtaaatc tggataatcc
     3241 acacaattga cgatgatatg gaattcatca tttttcaaag tatagtttaa tagagtgccc
     3301 tcatatatat acagaaattg ataatctttt tttggaatgg tataagttgt catattattt
     3361 taccttttca aaaacaatga gtttaagatt ttttgagtcc aagttggttc tactactctt
     3421 atgaaatttc agttgtttaa actcttcctt taacggccca ttatatttta accttaatcc
     3481 cactcgagcc agttctcctt catttggaat tcctcttaaa tacttattgt ttgatgtgcc
     3541 attaatcaca atttctcccc cttcttttgt aatacgtgct gcttcaagaa gataatccat
     3601 taaccgctca acatcccgcc tcttccaaaa ccgcgcgcgc ggccaaatcc gccgtatctt
     3661 ttttcaacag caggtgcaac gccctgaaat cctgttgcag cgcggcaacc ttatcggggt
     3721 gttcgtacca gtccgccaac gccgccgcca gtttttccgg ttttgcttca gattgcaata
     3781 attccggcac agcctcctta cccaacagga tattcggcag gccgacatgc ggcactttga
     3841 ttttgcgttt cacataagca taagtcagcg gcgaaatctt gtagctgatg accatcggac
     3901 gcttgcacaa cgccacctcc aaagtcgccg taccgctcgt taccagcacc gcatccgccg
     3961 ccctgcacac tgtttcagac tgtctgtcga ttaccgtcag cggcaatccg gcaaactccg
     4021 gccgctgcaa aacttccgcc aaacgccgct tcgtcgcctc cgttgcggca ggcagcagga
     4081 agcatgcggc aggatagcgt tccaacaaca ataatgccgt ctgaaaaaac accggcgcca
     4141 tatagtcgat ttcgctgacg cggctgccgg gcagcagggc gaatacgggg atgccggcat
     4201 ccacgcccaa agtttgccgc gccgtttcac ggtcgtcttc caagggcata agctgcgcca
     4261 tcggatgacc gacaaactcc gcacgtccgc ccgcatcgag ataaagctgc ggctccatcg
     4321 ggaacaggca caacacgcgg ttgacctgat gcacgatttt gcccacacgt tcccgccgcc
     4381 acgcccaaac cgacggactg acataatgca cggtcggaat ccccgaccgt ttgagccgct
     4441 ccgccacacc cagattaaaa tcgggcgcat cgataccgac aaagacatca ggtttcaacg
     4501 acagcaaatc ccgtaccagc cccctgcgta tccgtaaaat ttccggcagc cgcctgacca
     4561 cttcgacaaa gccgcgcacc gccagccgct cctgatcata aaggctctcg aaaccttccg
     4621 ccttcatcag ttcgccgccg ataccgacaa aacgcgcctg cggacaacgc ttgcggatgg
     4681 cgcgtatcag gtgcgccccc aacaggtcgc ccgacgcttc gccgacactg acggcaatca
     4741 aagggctttt tttatcagcc atattcgtct gtccccacat actttcacgt cccgatgccg
     4801 tctgaaggct tcagacggca tcggggttca cattttcgtc ccgttttcca acatcaccgc
     4861 aaactgtgcc aaatccggcg gcagccccac ctttaacacc agcggctcgc ccgtgagcgg
     4921 atggttcagg tgcagctcgg acgcgtgcaa aaacatccgc ttcaaaccca acttctgcaa
     4981 acgccggttc gcctgataat cgccgtagcg ttcgtcgccc gcaatcggac agccttgaga
     5041 ttgcaggtgg acgcggattt ggtgcgtgcg ccccgttttc aacgtcgccc gcaccaaagt
     5101 caggtgcgac agcccgacac cgtgcaaaat gccgtctgaa aaacggctta acacacggaa
     5161 caccgtatgc gccgactgcc cgtccgcact gacgcgcacc atcttttcgc cctgtgcgcc
     5221 cgtatatttg gacagcggca gcctgacgtg gaaattatcg tccggcagtt tgcccacccc
     5281 cagcgcaagg tagatttttt tggggtggtc gttacggatg gcttcgtgaa gtttgacgag
     5341 cgcgctgcgt ttcttcgcca ccatcaacaa accgctcgta tccttgtcca aacggtggac
     5401 gagttcgaga taacgcgcct ccggacgggc gcggcgcaac tgttcgataa cgccaaaact
     5461 cacgccgctg ccgccatgca ccgccacgcc ggaaggctta tcgatcacca aaagcgcgtc
     5521 gtcctcatac accgcctcaa acgcacgcgc cggtacggcg gcacgccttt cagacggcat
     5581 ttccttctcc gccacgcgca caggcggaat ccgcaccgta tccccctccg caatacggct
     5641 gtcgggtttg cagcgtttct tgttcaaccg cacctcgccg gcgcggataa tgcggtggat
     5701 atggctcttg ggaaccccct tgaggatttt tatcagatag ttatcaaggc gttgccccgc
     5761 ctcgtgttcg gcaaccccta tcaggctgac cgaatctttg cttatttcgt gcgttttcat
     5821 ctataatccg aacatccgtt tcagcaaaaa gcgcgcccgc cgcgcccccc tgaaacggtt
     5881 tactttaaaa cggaatttta tattaaaaac gcactccgca aagcattttc cctgcgcctg
     5941 ttcccagccg gcaggcgcag aacgtagtca agtttgaatt gattttgccg tttcggcgcg
     6001 gaagtaagac ggcagccgga ccccaagtcc caaacgcaaa ccgcccaaaa cacaggcatc
     6061 acgataacaa gaagaagccg gcacccattt ttccgaccgc agcgttcgga acgcggcaga
     6121 caaaccgccg catcgatctt tatagccttc tgtatccgac ccttccgcac acggttctcc
     6181 ggaacgtgca tccctgcgga ttttcaactc aaaaactgat taccggtttt atccgaagac
     6241 atcggaaaac ccagccttac ggaatgccga accggaccgg cgcgcccgaa tgccgcccgt
     6301 ccacgaggtg atcatgaaaa gaatgttatt taacgcaacg caggccgaag agctgcgcgt
     6361 tgccatcgtc gacggacaaa acctgctgga tttggacatc gaaacgctgg gcaaagaaca
     6421 gcgcaaaggc aatatctaca aaggcatcat tacccgcatc gagccgtcgc tggaagcgtg
     6481 tttcgtggat tacggaaccg accgccacgg cttcttgccg tttaaagaag tatcgcgttc
     6541 gtatttccgc gactacgaag gcggcagggc gcgtattcag gacgtgctca aagaaggcat
     6601 ggaagtcatc gtccaagtcg aaaaagacga gcgcggcaac aaaggcgcgg cactgaccac
     6661 cttcatcagc ctcgccggcc gctatctcgt actgatgccg aacaacccgc gcggcggcgg
     6721 cgtatcccgc cgtatcgaag gtgaagagcg tcaagagttg aaagccgcca tggcggaact
     6781 cgacattccg aacggcatga gcatcatcgc ccgcaccgcc ggcatcgggc gcagcgcgga
     6841 agagttggaa tgggacttga actacctcaa acaactctgg caggcgattg aagaagcagg
     6901 aaaggcgcat catgacccct acctgctctt tatggaaagc tcgctgctca tccgcgccat
     6961 ccgcgactat ttccgccccg acatcggcga gattttggtg gacaatcaag aagtttacga
     7021 ccaagtttcc gagttcatga gctatgtcat gccgggcaat ataggccgtc tgaaactcta
     7081 cgaagaccac acgccgctgt tttcccgctt ccaaatcgaa caccaaatcg aaagcgcgtt
     7141 ctcgcgcagc gtcagcctgc cttccggcgg cgcgatcgtc atcgaccaca ccgaagccct
     7201 cgtctccatc gatgtcaact ccgcccgtgc cacacgcggc gcggacatcg aagacaccgc
     7261 gttcaaaacc aatatggaag ccgccgaaga agtcgcccga caaatgcgcc tgcgcgactt
     7321 gggcggtttg gtcgtcatcg acttcatcga catggaaaac cccaaacacc agcgcgatgt
     7381 ggaaaacgtc ctgcgcgacg cgctcaaaaa agaccgcgcc cgcgtgcaaa tgggcaaact
     7441 ttcgcgtttc ggacttttgg aattgagccg ccaacgcctg aaacccgcct tgggcgaaag
     7501 cagccacgtc gcctgtccgc gctgcgccgg caccggcgtg attcggggca tcgaatccac
     7561 cgccctgcac gttttgcgta tggttcaaga agaagcgatg aaagacaaca ccggcgaagt
     7621 gcgcgcccaa gtgcccgtcg atgtcgccac cttcctgttg aacgaaaaac gcgccgagct
     7681 gtttgcgatg gaagagcgtt tggatgtcaa cgtcgtcctg attccgaaca tccacctcga
     7741 aaatccgcac tacgaaatca accgcatccg caccgacgac gtagaagaag acggcgaacc
     7801 gagctacaag cgcgtcgccg agcctgaaga agacgaatcc gccaaaccgt tcggcggcga
     7861 aaaagccaaa gccgcccgtc ccgaacccgc cgtcaaaggc gtgcgccaca ccagccccgc
     7921 cccgactgcc gcccccgaga aaaaaacctc ttggtgggac agcttcaaag catggctcaa
     7981 acgcattttc ggcggcagca aaacccaagc cgcacccgct gccgaaacct ccgaaaaacg
     8041 cagcacggca aaccgcagcg gcagccgcgc caacaaccgc cgccaaaacc cgcgccgcag
     8101 caaacgcgaa ggcagcaaag tggaagtccg cgaagtggca ggcaaaactg ccggacagga
     8161 agcgcgtgcc gacaaagccg aaacgcgcaa caacggcaac cgccgccgca acgaacgcgg
     8221 cgaccgtgcc gccggacgcg ccgacgaagc ggaaatccaa gaccgcaaca tacagcctgc
     8281 cgcaaccgtt gcagatgccg caccgtccga aaccgaagtg caaaccggaa aacgccgccg
     8341 caacggcagc cgcagcgaac gcggccaaac cgcgccggaa accgccgccg ttgccgaaac
     8401 aaccgttcag acagcggaaa acacgccgtc cgaaccgcat accgcagaag acaaaggcag
     8461 caagcccaaa tccgaacgca accgccgcga acgcgacagc cgagatgcca aagaacgccg
     8521 cgagcgcaac aatcagcgcg accgccgtca aaacggcaaa aaacgcaata ttccgtctgc
     8581 cgccaaaatc gagcagtacc tgaatattca cgacaccgcc gacaaagtcc gttccgccgc
     8641 cgcgcacgtt ttcggcgaaa ccgacgcaaa cgcgccgatt accgtcagca ttgccgatcc
     8701 ggttgcagaa agggatcttc cgacagcatc tcccgccgtt tcaaacggcg acgcaccggt
     8761 ttatgatgcg gcggaaaaaa tccgccgtgt caccgccgcc atcctgcccg aaggcgtagc
     8821 accgaaagcc gaagcacagg aaatgccgtc tgaaaccgca acctttacgg ctgcggcgga
     8881 acaggcacgg gaaaccgcac aaaccggcgg actcgtcctg atcgaaaccg accctgccgc
     8941 attgaaggca tgggcggcac aacccgaagt ccaaaccgga cgcggtttgc gccgttccga
     9001 acagcccaag ccgtctgaag tcgcaaccgt ccctgccgaa gaaatgatcc aagtcgaaac
     9061 ccggcaaggc tgaaccgacg gcggcaaaaa gaggttctgt tccgcagaac ctctttttta
     9121 catagattcg gatacctgca atgccgtctg aaacttcgcc attcccgtca ttcccgcaac
     9181 ctttcgtcat tcccatgatt cccgcaacat tccgtcattc ccgtgaaaac gggaatctag
     9241 aacctcaaac tttcagataa tctttgaata ttgccgctgc ccgaaggtct ggattcccgc
     9301 aacattccgt cattcccgtg aaaacgggaa tctagaacct caaactttcg gataatcttt
     9361 gaatattgcc gtcgcccgaa ggtctggatt cccgcaacat tccgtcattc ccgtgaaaac
     9421 gggaatctag aacctcaaac tttcagataa cctttgaata ttgccgctgt ccaatggtct
     9481 ggattcccgc ctgcgcggga atgacggttt agaagttgcc cgaaacctca aaaaaaccga
     9541 aaccgaacaa gccggattcc cgcaacattc cgtcattccc gtgaaaacgg gaatctagaa
     9601 cctcaaactt tcggataatc tttgaatatt gccgtcgccc aaaggcctgg attcccgcct
     9661 gcgcgggaat gacggcggag ggtggacgat gccgtctgaa acttcgccat tcccgtcatt
     9721 cccgccacct ttcgtcattc ccgtgaaaac gggaatctag aacctctaaa ctttcagata
     9781 atctttgaat attgccgtcg cccaaaggcc tggattcccg cctgcgcggg aatgacggtt
     9841 tagaagttgc ccgaaacctc aaaaaaaaaa accgaaaccg aacaagccgg attcccgcaa
     9901 cctttcgtca ttcccgtgaa aacgggaatc tagaacctca aactttcaga taatctttga
     9961 atattgccgt cgcccgaagg tctggattcc cgcctgcgcg ggaatgacga tttggaaatt
    10021 acccgaaacc caaaaacaac tgaaaccgaa cagaccggat tcccgcctgc gcggaaatga
    10081 cgggtctttt atcatcttta aaggctgccg cgcgccatct cgacggcggt ctccacggca
    10141 gttatcaggc tgccggaatc cgccctgccg gttgccgcca aatcgagcgc ggtgccgtga
    10201 tcgacggagg tgcggataaa gggcagcccc agtgtgatgt tcacgccctg cccgaaactg
    10261 tggtatttca acacgggcag cccttggtcg tggtacatcg ccaatacggc atccgcacct
    10321 tcgagcataa acggttggaa caatgtgtcc gccggatacg ggccggcgag gtttatcctt
    10381 tcgcggcgca ggttttcgag cgcggggata atggtgtcgg tttcttcgtg tccgaggtgt
    10441 ccgccctcac cggcgtgggg gttgagtccg gcgaccagga ttttgggatt tttgatgcag
    10501 aatttgtgtt ttaagtcgtg atgcaaaatg cgtgcgacgc tttcaatcag cggttgcgtg
    10561 atggcggcgg caacgtcttt cagcggcagg tgggtcgtta cgagggcgac gcgcaggcct
    10621 ttgccggcaa gcatcatcac gacctgcccc gtgccgcttt tttccgccag atattcggtg
    10681 tgtccgctga aaaaacctgt gcttgcgcgc gcgtcgttga tgatgccttt gtgcagcggc
    10741 gcggtaacga tgccgtcgaa aatgccgtct gaaatgcctg cgagcgcggt gtccaaaagt
    10801 tgcagcacat aggcggcgtt ggcgggattg agccgccctg cctcaaccgc ttcggcggca
    10861 gggatgtgca gcacttccag ctcgccgtat gccgcgccgc ctgattccgg atcgaagtcg
    10921 cgcaagacga cgcttttgcc caaggcttcg gcgcgcgcgc gcaataggtt tttgtcgccc
    10981 aataccgcgc agcggcaggg caggcgtgca aacgccaagt ccaaacaaat atcggggccg
    11041 atgccggcag gctcgccgga agtaacggca aaaacaggct gtttcatcgt gtttgctcca
    11101 aacaaaatgc gattctaacg ccgcagccgc gcggcgatgt aaatttttct gattttgttg
    11161 acaatctgct agaatgggcg tttacaaaat ttaaaccctg cttgcatacc gccaatatgt
    11221 gcgagtttca actttaagga agcgatatga acgagaactt taccgaatgg ctgcacggct
    11281 gggtcggcgc catcaacgat ccgatgtggt catacttggt ttatatgctt ttgggtacgg
    11341 ggcttttctt caccgtaacc acgggctttg tccaattccg cctgttcggg cgcagcatca
    11401 aagaaatgct cggcggccgc aaacaggggg acgaccctca cggcatcacg ccgtttcagg
    11461 catttgtaac cggccttgcc agccgcgtgg gtgtgggcaa catcgcgggc gtggcaatcg
    11521 ccatcaaagt cggcgggccg ggcgcggtgt tttggatgtg ggtaaccgcc ttaatcggta
    11581 tgagttcggc gtttgtcgaa tcttcgctgg cgcagctctt taaagtccgc gactacgaca
    11641 accaccattt ccggggcggc cccgcctact acatcactca agggctgggg cagaaatggc
    11701 tgggcgtgtt gttcgccctg agcctgattt tctgtttcgg ctttgtgttt gaagcggttc
    11761 agaccaatac catcgccgat accgtcaaag cggcatgggg ttgggagcct cattatgtcg
    11821 gcgtcgccct ggtgatttta accgcgccga ttatcttcgg cggcatcagg cgcatatcta
    11881 aagcggcgga aatcgtcgtc cccctgatgg cggttttgta cctctttatc gcgcttttca
    11941 tcattttgac caatattccg atgattccgg acgtgttcgg tcagattttt tcgggcgcgt
    12001 tcaaattcga cgcggcagca ggcggcttac tcggcggtct gatttcgcaa acgatgatga
    12061 tgggcatcaa acgtggcctg tattccaacg aggcgggtat gggttccgcg ccgaacgccg
    12121 ccgccgccgc cgaagtgaaa caccctgttt cgcaaggtat gattcaaatg ctgggcgtgt
    12181 ttgtcgatac catcatcgtt tgttcttgca ccgccttcat catcttgatt taccaacagc
    12241 cttacggcga tttgagcggt gcggcgctga cgcaggcggc gattgtcagc caagtggggc
    12301 aatggggcgc gggcttcctc gccgtcatcc tgtttatgtt tgccttttcc accgttatcg
    12361 gcaactatgc ctatgccgag tccaacgtcc aattcatcaa aagccattgg ctgattaccg
    12421 ccgttttccg tatgctggtt ttggcgtggg tctatttcgg cgcggttgcc aatgtgcctt
    12481 tggtctggga tatggcggat atggcgatgg gcattatggc gtggatcaac cttgtcgcca
    12541 tcctgctgct ctcgcccttg gcgtttatgc tgctgcgcga ttacaccgcc aagctgaaaa
    12601 tgggcaaaga ccccgagttc aaactttccg aacatccggg cctgaaacgc cgtatcaaat
    12661 ccgacgtttg gtaaatcccg cccttaccag agccgcttcc ccgcgaagcg gcttttccct
    12721 ttccgcacac tgtaaaaaca gggcgaacaa gcgtacaatc ccaacccttt acttttgaat
    12781 ccatttcgtt tttcagacgg catattgaat atagtggttt aacaaaaatc aggacaaggc
    12841 gacgaagccg cagacagtac aag
//
LOCUS       DAXHSF010000041        12331 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.041, whole genome shotgun sequence.
ACCESSION   DAXHSF010000041 DAXHSF010000000
VERSION     DAXHSF010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12331
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.041"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            318..1055
                     /locus_tag="ACFM8S_000879"
     CDS             318..1055
                     /locus_tag="ACFM8S_000879"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFC6313971.1"
                     /translation="MIILDKVSKHYQTRDKTRFAAVEPTSLEIRDGEIFGLMGYSGAG
                     KSTLLRLINLLERPDSGKVNVCGQELTALDAAALRQARQNIGMVFQQFNLLNNRTVAG
                     NVAFPLEIAGWPSEKIEARVAECLEIVGLTERAGHYPAQLSGGQKQRVGIARALASKP
                     QVILADEPTSALDPATTRSVLECLEDINKRFNVTIVIVTHEMSVIRRLCDRAALLDKG
                     KVVEIVEVRGNQIHARSDIGRELIRED"
     gene            1057..1743
                     /locus_tag="ACFM8S_000880"
     CDS             1057..1743
                     /locus_tag="ACFM8S_000880"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFC6313972.1"
                     /translation="MADLTFQQAVSNIIGMKGEIVRALGETFVMVGLSTTFAVVFGTL
                     LGVLLFVTSSRQLHYNKAVNFLLDNLVNLMRAFPFVILMIAMIPATRAIVGSTIGPVA
                     ASLVLSVSGLFYFARLVEQNLREVPKGVIEAAAAMGAPPVAIIRKVLLNEARAGMVSS
                     ITVLAIGLLSYSAAAGMIGGGGLGDLAIRYGYYRYQTEVIVFIVALLVLLVILIQSTG
                     NALARKLDKR"
     gene            1902..2765
                     /locus_tag="ACFM8S_000881"
     CDS             1902..2765
                     /locus_tag="ACFM8S_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFC6313973.1"
                     /translation="MKTFFKTLSAAALALILAACGGQKDSAPAASASAAADNGAAKKE
                     IVFGTTVGDFGDMVKEQIQPELEKKGYTVKLVEFTDYVRPNLALAEGELDINVFQHKP
                     YLDDFKKEHNLDITEVFQVPTAPLGLYPGKLKSLEEVKDGSTVSAPNDPSNFARVLVM
                     LDELGWIKLKDGINPLTASKADIAENLKNIKIVELEAAQLPRSRADVDFAVVNGNYAI
                     SSGMKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVTEAYNSDAFKAYAHKRFEGYK
                     SPAAWNEGAAK"
     gene            complement(2828..3397)
                     /locus_tag="ACFM8S_000882"
     CDS             complement(2828..3397)
                     /locus_tag="ACFM8S_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFC6313974.1"
                     /translation="MVRRLIIGISGASGFQYGVKALELLRAQDIETHLVVSKGAEMAR
                     ASETAYARDEVYALADFVHPIGNIGACIASGTFKTDGMLVAPCSMRTLASVAHGFGDN
                     LLTRAADVVLKERRRLVLMVRETPLNLAHLDNMKRVTEMGGVVFPPVPAMYRKPQTAD
                     DIVAHSVAHALSLFGIDTPDSTEWQGMAD"
     gene            3555..4415
                     /locus_tag="ACFM8S_000883"
     CDS             3555..4415
                     /locus_tag="ACFM8S_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFC6313975.1"
                     /translation="MAKVKGGLGRGLDSLLANGADNSSGDRLTTVAVKDIRPGRYQAR
                     VQIDDEALQELADSIKAQGVIQPVIVREHGLSRYELIAGERRWRAAQIAGLTEIPAVI
                     KTISDETALAMGLIENLQRENLNPIEEAQGLKRLADEFGLTHETIAQAVGKSRSAISN
                     SLRLLSLPEPVQEMLYQRRLEMGHARALLTLPVVEQLELAQKAVKNGWSVREVERRSQ
                     AALQNKRPEPKKTAAADIGRLNDLLTEKLGVNAEIKTANHKKGRIILHFDTPETFDHI
                     LKQLGIDYRP"
     gene            4551..4889
                     /locus_tag="ACFM8S_000884"
     CDS             4551..4889
                     /locus_tag="ACFM8S_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6313976.1"
                     /translation="MNTRIIVSAAFVALALAGCGSINNVTVSDQKLQERAAFALGVSP
                     NAVKISNRNNEGIRINFTATVGKRVSQCYVTSVISTIGVTTSDAICLGGGTHKGKSQC
                     NALLKAAGRC"
     gene            5257..5610
                     /locus_tag="ACFM8S_000885"
     CDS             5257..5610
                     /locus_tag="ACFM8S_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HFC6313977.1"
                     /translation="MKQIIILQSAVLSICAAVAFAVWGFAGFLSAVGGGLSYLLPTFV
                     AVLLLKLFRGNPFLQSRMFVFGEILKVVLSLLSMLAVFAIWHQSLVFAPFLMGLLGVS
                     HLVFLVLLRVKDYGR"
     gene            5600..6466
                     /gene="atpB"
                     /locus_tag="ACFM8S_000886"
     CDS             5600..6466
                     /gene="atpB"
                     /locus_tag="ACFM8S_000886"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFC6313978.1"
                     /translation="MAGETITAADYIKHHLQSLTSLSDVTQGQGLKNIADFSFINLDA
                     VFFAVLLGVIGSFLLWRGAKKATAGVPGRFQAAVEILFEFVDDMCKSIIHNEKSRKAV
                     APLGLTLFVWIFLMNAMDMLPVDLLPMVWQGITGNHHALLRVVPTADLNTTLALAVGV
                     LLICIYYNIKIKGLGGWFHELFSAPFGAKLAPANFLLNLVEFLSKTVSHGMRLFGNMY
                     AGELVFLLIALLGGAWAASGSVEVMDPILFVFHIIAGLAWAIFHILVITLQAFIFMAL
                     AFVYIGQAHDAH"
     gene            6523..6759
                     /gene="atpE"
                     /locus_tag="ACFM8S_000887"
     CDS             6523..6759
                     /gene="atpE"
                     /locus_tag="ACFM8S_000887"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFC6313979.1"
                     /translation="MGLIAIACGLIVALGALGASIGIAMVGSKYLESSARQPELIGPL
                     QTKLFLIAGLIDAAFLIGVAIALLFAFVNPFAGA"
     gene            6830..7300
                     /locus_tag="ACFM8S_000888"
     CDS             6830..7300
                     /locus_tag="ACFM8S_000888"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFC6313980.1"
                     /translation="MNINATLFAQIIVFFGLVWFTMKFVWPPIAKALDERAAKVAEGL
                     AAAERGKSDFEQAEKKVAELLAEGRNQVSEMVANAEKRAAKIVEEAKEQASSEAARIA
                     AQAKADVEQELFRARESLREQVAVLAVKGAESILRSEVDASKHAKLLDTLKQEL"
     gene            7305..7838
                     /locus_tag="ACFM8S_000889"
     CDS             7305..7838
                     /locus_tag="ACFM8S_000889"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFC6313981.1"
                     /translation="MAEFATIARPYAKALFGLAQEKNQIESWLGGLEKLAAVVQEGKV
                     ASLIDRPETNASEKADILIDLVGLKDKELKNFVIVLAGQKRLSILPEVYAQYQDLTLS
                     FNHIKSAIIYSAYPLTDKQVGELAQMLNKRFDSELKISVEIEPELIGGIKVEVGDQVL
                     DLSVQGKLSALYTTMTN"
     gene            7849..9396
                     /gene="atpA"
                     /locus_tag="ACFM8S_000890"
     CDS             7849..9396
                     /gene="atpA"
                     /locus_tag="ACFM8S_000890"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFC6313982.1"
                     /translation="MQLNPAEISDLIKAKIENLSVNAEVRTRGTVISVTDGIVRIHGL
                     SDAMQGEMLEFPGNTFGLAMNLERDSVGAVVLGEYEHIKEGDTVTCTGRILEVPVGRE
                     LVGRVVDALGRPIDGKGPINTTLTAPIEKIAPGVIARKSVDQPMQTGLKAIDSMVPVG
                     RGQRELIIGDRQTGKTAVALDAIVNQKGTGVICIYVAIGQKASSIANVVRKLEEHGAM
                     EHTIVVAATASEAAALQYIAPYSGCTMGEFFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVSENEVEKLTNGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     IRLALAQYRELAAFSQFASDLDEATRKQLEHGEVVTELMKQKQFSTLNTAEMALTLWA
                     INNGSYSDVPVAKALAFESEFLSFVRTQHPEVLEAVNASGAMSDESEKTLEAAMKSFK
                     SSYAYQA"
     gene            9421..10296
                     /gene="atpG"
                     /locus_tag="ACFM8S_000891"
     CDS             9421..10296
                     /gene="atpG"
                     /locus_tag="ACFM8S_000891"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFC6313983.1"
                     /translation="MAVGKEILTKIRSVQNTQKITKAMQMVSTSKMRKTQERMRLARP
                     YAEKVRMVMSHLAQTNTDHGIPLLESHREIRRVGFILITSDKGLCGGLNANVLKKFLA
                     QVQEYRNQGIEEEIVCLGSKGLMACQSIGLNVVASAVNLGDTPKMEMLLGPLTELFQR
                     YEKHEIDRIHLVYSGFVNTMRQEPRMEVLLPIGENVIGDSAPKSPFSWEYRYEPTALA
                     VLEYLVRRYLESVVYQALSDNMASEQAARMVAMKAATDNAGNAIKELRLVYNKSRQAA
                     ITTELSEIVAGAAAV"
     gene            10334..11731
                     /gene="atpD"
                     /locus_tag="ACFM8S_000892"
     CDS             10334..11731
                     /gene="atpD"
                     /locus_tag="ACFM8S_000892"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFC6313984.1"
                     /translation="MSQGKIVQIIGAVVDVEFPRDMIPRVYDALKLDENGLTLEVQQL
                     LGDGVVRTIAMGSSDGLKRGMTVSNTGAPITVPVGKGTLGRIVDVLGTPVDEAGPIDT
                     DKSRAIHQAAPKFDELSSTTELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFAGVGERTREGNDFYHEMKDSNVLDKVAMVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEKDENGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTL
                     AEEMGRLQERITSTQTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRDIASLG
                     IYPAVDPLDSTSRQLDPMVLGQEHYDVARGVQSTLQKYKELRDIIAILGMDELSDEDK
                     LTVMRARKIQRFLSQPFHVAEVFTGSPGKYVALRDTIAGFKAILNGEYDHLPEQAFYM
                     VGSIEEAVEKAKTLN"
     gene            11742..12164
                     /locus_tag="ACFM8S_000893"
     CDS             11742..12164
                     /locus_tag="ACFM8S_000893"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFC6313985.1"
                     /translation="MSIMQVEVVSSEQKIYSGEATFIVVPTVQGELGIYPRHEPIMSL
                     VRPGALRLTVPGEDKEVLVAVSGGVLEVQPDKVTVLADVAVRSAEMDQARAEEAKKAA
                     EAGISQAKDDKALAEAHKALAAAIAQLKTLDYIRSHKK"
BASE COUNT         3043 a         3139 c         3159 g         2990 t
ORIGIN      
        1 atcggttccg tactatttgt actgtctgcg gcttcgtcgc cttgtcctga tttttgttaa
       61 tccactatat gtttttcaat tatttgccgt tttggtgcga accgctgcct ttgcccgttt
      121 cagacggcat tgtccgaaat ggttgcccgc ttcctgcttt attgacaaaa aaatgctttc
      181 ccgataatat cctacgaaaa ttaacctgcc gatttgacac agcttgcggg cataacagct
      241 aaagcgttcc gacaatttca gctttatctt ccgcgcccgt tgtgtccgac atcgggcttt
      301 gttgtatgga aaagacaatg attattttgg acaaggtttc caagcattac caaacgcgcg
      361 acaagacccg ttttgccgcc gtcgagccga ccagcctcga aatccgcgac ggcgaaatct
      421 tcgggctgat gggttattcg ggtgcaggca aatccaccct gttgcgcctg attaacctgt
      481 tggaacgtcc cgacagcggc aaggtcaacg tctgcggaca agagctgacc gcgctcgatg
      541 ccgccgcatt gcgtcaggct cggcagaata tcggcatggt gtttcagcag ttcaacctgt
      601 tgaacaaccg caccgtcgcc ggcaacgtcg ccttcccatt ggaaatcgcc ggatggccgt
      661 ctgaaaaaat cgaagcgcgc gttgccgaat gtctcgaaat cgtcggactg accgaacgcg
      721 ccggtcacta tcccgcccag ctttccggcg gacaaaaaca gcgcgtcggc atcgcccgcg
      781 cgctcgcatc caaaccccaa gtcatcctcg ccgacgaacc cacttccgcc ctcgaccccg
      841 ccaccacgcg cagcgtcttg gaatgtttgg aagacatcaa caaacgattc aacgtaacca
      901 tcgtcatcgt aacccacgaa atgagcgtca tcagacgact gtgcgaccgc gccgccctgt
      961 tggacaaagg caaagtcgtc gaaatcgtcg aagtacgcgg caaccaaatc cacgcccgat
     1021 ccgacatcgg gcgcgaactg attcgggagg actgatatgg cagacttaac attccaacaa
     1081 gccgtttcca acatcatcgg tatgaaaggc gaaatcgtcc gcgcattggg cgaaaccttc
     1141 gtgatggtcg gcttgtccac cacattcgcc gtcgttttcg gcacgttgct gggcgtgttg
     1201 ctcttcgtaa cttccagccg ccaactgcat tacaacaagg cggtgaactt cctgctcgac
     1261 aacctcgtca acctcatgcg cgccttcccc ttcgtcattt tgatgattgc gatgataccc
     1321 gccacacgcg ccatcgtcgg cagcaccatc ggtccggttg ccgcctcgct ggtgttgagc
     1381 gtgtcgggat tgttttattt tgcccgtttg gtggaacaaa acctgcgcga agtccccaaa
     1441 ggcgtgattg aagccgccgc cgctatgggt gcgccgccgg ttgccatcat ccgcaaagtc
     1501 ctcttaaacg aagcgcgcgc gggcatggtt tccagcatta ccgtgcttgc catcgggctt
     1561 ttgtcataca gcgcggcggc agggatgata ggcggcggcg gcttgggcga cctcgccatc
     1621 cgctacggct actaccgcta ccaaaccgaa gtcatcgtct tcatcgtcgc cctcctcgtg
     1681 ctgctggtca tcctgattca aagcaccggc aacgcgttgg cgcggaaact cgacaaacgt
     1741 tgaacccaaa tgccgtctga acgccaaaac ccccaccgct atccgaaaaa tgctataaaa
     1801 tccccctgtt cgcggcaaat gccgtctgaa cgccgaatcc ggacggcagg actccctgcc
     1861 cgtcattttt gtttgaaact gccacaacat caggagaaaa tatgaaaacc ttcttcaaaa
     1921 ccctttccgc cgccgcactc gcgctcatcc tcgccgcctg cggcggtcaa aaagatagcg
     1981 cgcccgccgc atccgcttct gccgccgccg acaacggcgc ggcgaaaaaa gaaatcgtct
     2041 tcggcacgac cgtcggcgac ttcggcgata tggtcaaaga acaaatccaa cccgagctgg
     2101 agaaaaaagg ctacaccgtc aaactggtcg agtttaccga ctatgtgcgc ccgaatctgg
     2161 cattggctga gggcgagttg gacatcaacg tcttccaaca caaaccctat cttgacgact
     2221 tcaaaaaaga acacaatctg gacatcaccg aagtcttcca agtgccgacc gcgcctttgg
     2281 gactgtaccc gggcaagctg aaatcgctgg aagaagtcaa agacggcagc accgtatccg
     2341 cgcccaacga cccgtccaac ttcgcccgcg tcttggtgat gctcgacgaa ctgggttgga
     2401 tcaaactcaa agacggcatc aatccgctga ccgcatccaa agcggacatt gccgaaaacc
     2461 tgaaaaacat caaaatcgtc gagcttgaag ccgcgcaact gccgcgtagc cgcgccgacg
     2521 tggattttgc cgtcgtcaac ggcaactacg ccataagcag cggcatgaag ctgaccgaag
     2581 ccctgttcca agaaccgagc tttgcctatg tcaactggtc tgccgtcaaa accgccgaca
     2641 aagacagcca atggcttaaa gacgtaaccg aggcctataa ctccgacgcg ttcaaagcct
     2701 acgcgcacaa acgcttcgag ggctacaaat cccctgccgc atggaatgaa ggcgcagcca
     2761 aataaggcag tcgtataaaa tgatgccgtc tgaaccgcat tccgtgttca gacggcattt
     2821 ttgtccttta atccgccatt ccctgccatt ccgtcgaatc cggtgtatcg attccgaaca
     2881 gcgacaaagc gtgtgcaaca ctgtgcgcca ctatgtcgtc cgccgtctgc ggtttgcggt
     2941 acatcgcagg aacaggggga aacaccacgc cgcccatttc cgttacccgc ttcatattgt
     3001 ccaaatgggc aaggttcagc ggcgtttcgc gcaccatcag caccagccgc cgcctttcct
     3061 tcaaaaccac atccgccgca cgcgtcaaga ggttgtcgcc gaagccgtgc gcgaccgagg
     3121 caagtgtccg catcgaacag ggggcgacca gcatcccgtc cgttttaaac gtaccgctgg
     3181 caatgcacgc cccgatattg ccgatcggat gcacgaagtc cgccaaggca tatacctcat
     3241 ctctcgcata agccgtttcc gaagcgcgcg ccatctccgc acctttcgat accacaaggt
     3301 gcgtttcgat atcttgcgcg cgtaaaagtt ccaaagcctt cacgccgtat tggaaaccgc
     3361 tcgccccact gatgccgatt atcaaacgcc gtaccatcat ccgcctttcc cataaaaccg
     3421 cctgcaacgg caaaccggct attatagtga aaaaacagaa atccgataaa tacggataca
     3481 aattgtcggc aacacccaat atccgataaa atatccgatt taacatccta tctgaatagg
     3541 cacgggaggg cggtatggca aaagtaaaag gcggattggg gcgcggtttg gattcgctgc
     3601 tcgccaacgg cgcagacaac agcagcggcg accgattgac cacggttgcg gttaaagata
     3661 tccggcccgg ccgctatcag gcgcgtgttc aaatcgatga cgaagccttg caggagctgg
     3721 cagattcgat taaggcgcaa ggcgtgatac agcccgtcat cgtgcgcgaa cacggactgt
     3781 cccgatacga actgattgcg ggggagcggc gttggcgtgc cgcacagatt gccggcctga
     3841 ccgaaatccc cgccgttatc aaaaccatca gcgacgaaac cgcgttggca atgggtttga
     3901 tcgaaaacct ccagcgcgaa aacctcaacc ccatcgaaga agcacaaggc ttgaaacgcc
     3961 ttgccgacga gttcgggctg acccacgaaa ccatcgccca agccgtcggt aaaagccgaa
     4021 gcgcgatttc caacagcctg cgccttttaa gcctgcccga acccgtgcag gaaatgcttt
     4081 accaacgccg cctcgaaatg gggcacgccc gcgcactgct gaccctgccc gtcgtcgaac
     4141 agctcgaatt ggcgcaaaag gctgtcaaaa acggctggtc ggtgcgcgaa gtcgaacgcc
     4201 gcagccaagc cgcccttcaa aacaaacgtc ccgagcccaa aaaaactgcc gccgccgaca
     4261 tcggccgcct gaatgatttg ctgactgaaa aactgggtgt caacgccgaa atcaaaaccg
     4321 ccaaccataa aaaaggcaga atcatcctgc atttcgacac gcccgaaacc ttcgaccata
     4381 tcttgaagca attgggcata gattaccgac cttaattttg cgggatatac cgtctgaaat
     4441 atagagaata gctttccaga ttttaagtgg gaaatataat tctattgaca ttttttctgc
     4501 ttcacgtaag aatcgttttc ctgttttcat ttttaatttt cgaggaaatt atgaacacac
     4561 gcatcatcgt ttcggctgcg ttcgttgcgt tggcattagc aggttgcggc tcaatcaata
     4621 atgtaaccgt ttccgaccag aaacttcagg aacgtgccgc gtttgccttg ggcgtcagcc
     4681 caaatgccgt aaaaatcagc aaccgcaaca atgaaggcat acgcatcaac tttaccgcaa
     4741 ctgtgggtaa gcgcgtgagc caatgctatg ttaccagtgt aatcagcaca atcggcgtta
     4801 ccacttccga tgcaatttgt ttgggaggcg gaacgcacaa aggcaaaagt caatgcaatg
     4861 ctttgcttaa agcggcaggc cgttgctaat cctttattcg gaaaaggtcg tctgaaaata
     4921 ttttcagacg accttttatt tattgagcaa attccccaaa ctgccgacaa tccgaccgat
     4981 aaactgctac aatttcgcgt tgatttttta ttatttccac gtaaagcaaa caaatcacgt
     5041 aagaacatta aaaatgtaaa gaaattgcgg acaaactcct taatatcctg taattatatg
     5101 taatgtgtgt tagaatgaca cttgtcgccg ttattcaggc ggcgagcgcg gcggattggg
     5161 atacgttgtt tcacaaagat taactgtcgc ttgacgtaat aaggcttcta aattatatta
     5221 gccgcgcccc aaatccgagg cggaaacatt ccctttatga agcagattat catcctacaa
     5281 tccgccgtat tgtccatatg cgccgcagtt gcctttgctg tatggggttt tgccggattc
     5341 ctttcggcgg tcggtggcgg tttgtcctac ctgcttccca cgtttgttgc agttttactt
     5401 ttaaaacttt tcaggggaaa ccccttcctg caaagcagga tgttcgtctt cggagagatt
     5461 ttaaaagtag tgctgtcgct gttgtccatg cttgccgtat ttgcaatatg gcatcaatcg
     5521 ctggtgttcg ccccgtttct gatggggctg ctcggtgtca gccatttggt ttttttagta
     5581 ttgttgagag tgaaagatta tggcaggtga aaccattacc gctgccgact acatcaagca
     5641 ccacttgcag agcttgacca gtttgtcgga tgttactcag ggtcagggac tgaaaaacat
     5701 tgctgatttt tcgtttatta accttgatgc cgtctttttt gccgtcctgt tgggcgtaat
     5761 cggcagcttt ctgttgtggc gcggtgcgaa aaaagcaacg gcaggcgttc ccggacgttt
     5821 ccaggctgcc gtggaaatct tgtttgagtt tgtggacgat atgtgtaaga gcatcattca
     5881 caacgaaaag tctagaaaag ccgtcgcgcc gttgggtctg acgctgtttg tctggatttt
     5941 tctgatgaac gcgatggata tgctgccggt cgatttgctg ccgatggtat ggcagggcat
     6001 taccggcaac catcacgccc tgctgcgcgt cgtaccgact gccgatttga acaccacttt
     6061 ggcactcgct gtcggcgtgt tgctgatttg tatctattac aacatcaaaa tcaaaggatt
     6121 gggcggctgg tttcacgagt tgttcagcgc gccgttcggt gcaaaactcg cacctgcgaa
     6181 ctttctgttg aatttggtcg agtttctttc taaaaccgta tcccacggta tgcggttgtt
     6241 cggcaatatg tatgcgggcg aattggtatt cttgctgata gccttgcttg gcggtgcttg
     6301 ggcggcttcc ggcagcgtcg aagtcatgga tccgattctg tttgtattcc acattattgc
     6361 cggtttggca tgggcgattt tccatatttt ggtgattacc ctgcaggcgt ttattttcat
     6421 ggcgttggcg ttcgtctata tcggacaggc acatgatgcg cactgatttt cctaagtagt
     6481 ttgtttttgt agtttaacct ttgtttcttt aaggagttta aaatgggttt gattgctatc
     6541 gcatgtggtt tgatcgttgc attgggtgca ttgggtgcct ctatcggtat cgcaatggtc
     6601 ggttctaaat atttggagtc ttctgcccgt cagcccgaac tgatcggtcc gctgcaaacc
     6661 aaactgttcc tgattgccgg tttgattgat gccgcattct tgattggtgt cgccattgca
     6721 ctgctgttcg ccttcgttaa cccgtttgca ggtgcataat caggacggat tcgtccgttc
     6781 cgtacaggcg cagcctgttt gattaacccc agtacgaagg ttaagtaacg tgaatatcaa
     6841 tgcaacatta ttcgctcaaa tcatcgtctt tttcggtttg gtatggttta ccatgaaatt
     6901 cgtgtggccg ccgattgcaa aagctttgga tgagcgtgcc gcaaaagtcg ccgaaggctt
     6961 ggctgccgcc gagcgtggta aaagcgattt cgagcaggct gaaaaaaagg ttgcagaact
     7021 cttggcagaa gggcgtaatc aggtttccga aatggttgcc aacgccgaaa aacgtgccgc
     7081 caaaattgtc gaagaagcca aagaacaggc ttcttccgag gcggcgcgca ttgcagctca
     7141 ggcaaaggcc gatgtggagc aggaattgtt ccgcgcacgc gaatccctgc gcgagcaggt
     7201 tgccgtgttg gctgtcaaag gtgccgaatc tattttgcgc agcgaagtcg atgcttccaa
     7261 acacgcaaaa ctgctcgata ccctgaaaca ggagctgtaa tcttatggca gagttcgcaa
     7321 cgattgccag accttatgca aaggcattgt tcggtctggc tcaggaaaaa aaccaaattg
     7381 agtcttggtt gggcggactg gaaaaacttg cggcggttgt tcaggaaggg aaggtggctt
     7441 cattgattga ccgtcctgaa acgaatgctt cagaaaaagc agatatcctc atcgatttgg
     7501 tcggtttgaa agacaaggag ttgaaaaact ttgttatcgt cttggccggg cagaaacgtt
     7561 tgtcgatatt gccggaagta tatgctcaat atcaagactt gaccttatca ttcaaccata
     7621 tcaaatctgc catcatttac agtgcctatc cgttgaccga caaacaggtc ggcgagttgg
     7681 cgcaaatgct gaataagcgt ttcgacagcg agctgaaaat ctctgtcgaa atcgaaccgg
     7741 agctgattgg cggcataaaa gttgaagtgg gtgatcaggt tttggatttg tctgtacaag
     7801 gcaaattgag tgccttgtac acgactatga cgaattagga gagttttcat gcagcttaat
     7861 cctgctgaaa ttagcgattt gattaaagcc aagatcgaaa atctgtctgt aaatgccgaa
     7921 gtgcgtactc gtggtacggt gatttcggta actgacggta tcgttcgcat ccatggtttg
     7981 tcagatgcaa tgcaaggtga gatgctcgaa ttcccgggta acactttcgg cttggcgatg
     8041 aacctggagc gcgactccgt cggcgccgta gtgttgggcg agtacgaaca tattaaagaa
     8101 ggcgacacgg ttacctgtac cggtcgtatc ttggaagtgc cggtcgggcg cgaactggtc
     8161 ggacgcgtcg ttgatgcatt gggtcggcct atcgacggca aaggcccgat taatacaact
     8221 ttgaccgccc ctatcgaaaa aatcgcaccg ggtgtgattg cgcgtaaatc ggttgaccaa
     8281 ccaatgcaaa ccggcttgaa agccattgac tctatggttc cggttggtcg tggtcaacgt
     8341 gagttgatca ttggtgaccg tcaaaccggt aaaactgccg tagcactgga tgccatcgtt
     8401 aaccaaaaag gtacaggcgt aatctgtatt tatgtggcta tcggtcaaaa agcatcttct
     8461 attgccaacg tagtgcgcaa attggaagag catggcgcga tggaacatac tattgtagtt
     8521 gctgcaactg cttctgaagc cgctgcgctg caatatatcg ctccttactc cggttgtacc
     8581 atgggcgaat tcttccgtga ccgcggtgaa gatgcgttga tcgtatatga tgacttgtct
     8641 aaacaagccg ttgcataccg tcaaatttcc ctgctgctgc gccgtcctcc tggtcgtgaa
     8701 gcatatcctg gcgatgtatt ctacctgcac tcccgtctgt tggaacgtgc ggcccgtgtc
     8761 agcgaaaacg aagttgaaaa actgaccaat ggcgaagtaa aaggcaaaac cggttctctg
     8821 actgcattgc cgattattga aacccaagcc ggtgacgtat cggcattcgt accaaccaac
     8881 gtaatttcga ttacagacgg ccaaatcttc ttggaaaccg acttgttcaa cgccggtatc
     8941 cgtcctgcaa tcaatgccgg tatttccgta tcgcgcgtag gtggtgcagc acaaaccaaa
     9001 gtcatcaaaa aactgggtgg cggtatccgt ttggcgttgg cgcaatatcg tgaattggcg
     9061 gctttctcgc aatttgcatc cgatttggat gaagctacgc gcaaacaatt ggagcatggc
     9121 gaagtcgtaa ccgaactgat gaaacagaaa cagttcagta cgttgaatac ggctgaaatg
     9181 gctttgaccc tttgggcaat caacaacggt tcgtattctg atgttccggt tgccaaagct
     9241 ctggcttttg aatctgaatt tttgagcttt gtccgtaccc aacatccgga agttttggaa
     9301 gccgtcaatg cttcaggtgc aatgtccgac gagagcgaga aaacgcttga agcagccatg
     9361 aaatccttca aatcttctta cgcctatcag gcataaggct tgaagtgaaa ggagtctgaa
     9421 atggcagtag gaaaagagat tctcaccaaa atccgcagtg ttcagaatac ccaaaagatc
     9481 actaaagcga tgcagatggt gtcaacctct aaaatgcgga agactcagga acggatgcgt
     9541 ttggcgcgtc cgtatgccga aaaagtgcgt atggtgatga gccatcttgc gcaaaccaat
     9601 accgatcatg gtattccgtt actggaatct catcgggaaa tcagacgtgt cggttttatt
     9661 ttgattacgt ctgataaggg tttgtgtggc ggtttgaacg ccaacgtgct gaaaaagttt
     9721 ttggcacaag ttcaagagta tcggaatcaa ggtattgaag aggaaatcgt atgccttggc
     9781 agtaaaggtc tgatggcgtg tcagagcatt ggtctgaatg tggttgccag tgccgtaaat
     9841 ttgggcgata ccccaaaaat ggaaatgctg ctcggacctt tgacagaact cttccaacgg
     9901 tatgagaaac atgaaattga cagaatccat ctggtgtatt cgggttttgt caataccatg
     9961 cgtcaagaac cgagaatgga agtattgctg cctatcggtg agaacgtgat tggcgattca
    10021 gctcccaaat caccgttcag ctgggaatac cgctacgaac cgactgcact tgcagtgttg
    10081 gaatatctgg ttcgccgcta tttagagtct gtggtttatc aggcgttgag cgacaatatg
    10141 gcatccgaac aggcagcccg catggttgcc atgaaagctg caacagacaa tgcaggcaat
    10201 gccatcaaag agttgcgttt ggtatataac aaatcgcgtc aagctgcgat taccacggaa
    10261 ttgtcagaaa ttgtagcagg tgcggcggct gtctgatgcc gtctgaatcc tgtacggaaa
    10321 ttaggatacg ataatgagcc aaggcaaaat cgtacaaatt atcggtgcgg ttgttgacgt
    10381 ggaatttcca cgcgacatga ttccgcgcgt ttacgacgct ttgaaattag acgaaaacgg
    10441 tctgactttg gaagtccaac agcttttggg cgacggcgta gtccgtacca ttgcgatggg
    10501 cagctcggac ggtttgaaac gcggcatgac tgtgagcaat accggtgcgc ccattactgt
    10561 gccggtaggt aaaggtacgt tgggacgcat tgtcgatgta ttgggaacgc ctgttgacga
    10621 ggcaggtccg atcgataccg acaagagtcg tgccatccac caagccgctc ctaagtttga
    10681 cgaactgtct tccacaaccg aattgctcga aacgggcatt aaagtgattg acttgctgtg
    10741 tccgtttgcc aaaggcggta aagtaggtct gttcggcggt gccggtgtgg gtaaaaccgt
    10801 gaacatgatg gaattgatca acaacatcgc caaagcgcac agcggcttgt ccgtgttcgc
    10861 aggcgtgggt gagcgtaccc gcgaaggtaa cgacttctac cacgagatga aagattccaa
    10921 cgtattggat aaagtggcaa tggtttacgg tcagatgaac gaacctccgg gcaaccgttt
    10981 gcgcgtcgca ttgaccggtt tgaccatggc ggaatacttc cgtgacgaaa aagacgaaaa
    11041 cggcaaaggc cgcgacgtat tgttcttcgt ggacaacatc taccgttaca ctctagctgg
    11101 taccgaagta tccgcattgt tgggccgtat gccgtctgca gtgggctacc aaccgacatt
    11161 ggcagaagaa atgggtcgtt tgcaggagcg tattacctct acccaaaccg gttctattac
    11221 ttctatccaa gccgtatatg tacctgcgga cgacttgacc gacccgtctc cggcaacaac
    11281 gttcgcccac ttggacgcaa ccgtcgtatt gagccgcgat attgcctctt tgggtattta
    11341 cccggcagtc gatccgcttg attccacttc gcgccaactg gatccgatgg tcttgggtca
    11401 agagcactac gacgtggcgc gcggcgtaca gtccaccctg cagaaataca aagaattgcg
    11461 cgacatcatc gccatcttgg gtatggacga attgtctgac gaagacaaac tgaccgtaat
    11521 gcgtgcgcgt aaaatccaac gcttcctgtc ccaaccgttc cacgttgccg aagtgtttac
    11581 aggttctccg ggcaaatatg tcgccctgcg cgataccatt gccggcttca aagccatctt
    11641 gaacggcgaa tacgatcatc tgcccgagca ggcattctat atggtcggca gcattgaaga
    11701 agcggttgag aaagcgaaaa ccttaaactg aggaggtcgg catgagcatc atgcaagttg
    11761 aggtggtaag tagcgagcag aaaatctatt caggcgaggc aacatttatc gttgttccga
    11821 ctgtacaggg cgaactcggt atttatccgc gacacgagcc gattatgagt ttggtgcggc
    11881 cgggggcttt gcgtttgacc gttccgggcg aggataaaga ggttttggtt gctgtttccg
    11941 gcggtgtttt ggaagtacag cctgataaag taactgtctt ggcggatgtt gccgtccgca
    12001 gtgcagagat ggatcaggca cgtgcggaag aggcgaaaaa agccgcagaa gcgggcattt
    12061 cccaagctaa agacgataag gctttggcgg aggcgcataa agcattggct gctgcgattg
    12121 cacagcttaa aactttggac tatatccgtt cgcacaagaa ataaacttgt ttggtttgga
    12181 aaagcacgac cgtttggtcg tgctttttat tttccggatt taaattaaag actgtgtttg
    12241 gatatggcgt tggcaggcat agcctgcttt aaggtattca atagggcagc ctgccgccta
    12301 accgaaacat atagcggatt aacaaaaatc a
//
LOCUS       DAXHSF010000042        12097 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.042, whole genome shotgun sequence.
ACCESSION   DAXHSF010000042 DAXHSF010000000
VERSION     DAXHSF010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12097
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.042"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(42..971)
                     /locus_tag="ACFM8S_000894"
     CDS             complement(42..971)
                     /locus_tag="ACFM8S_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFC6313986.1"
                     /translation="MDSIIELRHLKTLLALEETGSVSLAAKRVFLTQSALSHQIRMLE
                     NHYGAPLFERKSTPLRFTPVGERLLRLAHELIPQVAVAERDLARITEGEAGELRIAVE
                     CHTCFDWLMPAMGEFRPMWPQVELDIVSGFQADPVGLLLQHRADLAIVSEAEKQSGIS
                     FQPLFAYEMVGICAKDHPLAAKTVWEAEDFIGETLITYPVPDEMLDLPKKILIPKNIN
                     PPRRHSELTIAIIQLVASRRGIAALPYWTVMPYLEKGYVVHRQITDDGLQSKLYAAIR
                     TEDTDKSYLNNFCQIIRERGFADLPGLSELEPV"
     gene            1102..1851
                     /locus_tag="ACFM8S_000895"
     CDS             1102..1851
                     /locus_tag="ACFM8S_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFC6313987.1"
                     /translation="MVLRRDFLTWCNETLQTALFKDYAPNGLQVEGREYIGKIVTSVT
                     ASRAAIDFAVEQKADLLLVHHGMFWKNELPTVTGWKKERIAALLRHDINMAGYHLPLD
                     AHPTLGNNAQLADRLGFATEKRFGEQNLLNSGSLKQAKTLGALAAHIETVLQRKPVVI
                     GKPEREIRRVAWCSGGAQGFFQTAIDEGVDLYLTGEISEAQYHLANETGTAFISAGHH
                     ATERYGVRALAESAAEVFGLEVCHFDENNPA"
     gene            1974..2555
                     /gene="petA"
                     /locus_tag="ACFM8S_000896"
     CDS             1974..2555
                     /gene="petA"
                     /locus_tag="ACFM8S_000896"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HFC6313988.1"
                     /translation="MDNQEINNGRRRFLTLATCGAGGVAALGVATPFVASFFPSEKAK
                     AAGAAVEVDVSKIEAGQLLTAEWQGKPIWVLNRTDQQLKDLKGLNGELTDPNSDAEQQ
                     PEYAKNETRSIKPNILVAIGICTHLGCSPTFRPDIAPADLGADWKGGFFCPCHGSKFD
                     LAGRVYKGVPAPTNLVVPPYKYLSDTTILVGED"
     gene            2574..3923
                     /locus_tag="ACFM8S_000897"
     CDS             2574..3923
                     /locus_tag="ACFM8S_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HFC6313989.1"
                     /translation="MANQTNSKAKALLGWVDARFPLSKMWKEHLSEYYAPKNFNFWYF
                     FGSLSMLVLVIQIVSGIFLTMNYKPDGNLNAYHLPAAFTAVEYIMRDVSGGWIIRYMH
                     STGASFFFIVVYLHMFRGLIYGSYKKPRELVWIFGSLIFLALMAEAFMGYLLPWGQMS
                     FWGAQVIINLFSAIPVIGPDLSTWIRGDFNVSDVTLNRFFALHVIAVPLVLLGLVVAH
                     IIALHEVGSNNPDGVEIKKLKDENGVPLDGIPFFPYYVVHDILAVTIFLIVFCAVMFF
                     APEGGGYFLEAPNFDAANALKTPPHIAPVWYFTPFYAILRAIPSFLGTQVWGVIGMGA
                     AVVLIALLPWLDKGEVKSVRYRGPIFKTALVLFIIAFIGLGILGAMVATDTRTLVARI
                     LSFVYFAFFLGMPFYTKLDTNKPVPERVTMSTTKQKIMFFVYVGITVVGAYLFATNI"
     gene            3938..4723
                     /locus_tag="ACFM8S_000898"
     CDS             3938..4723
                     /locus_tag="ACFM8S_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HFC6313990.1"
                     /translation="MKQTLKNWFAALLLAVPMSAAVASGGGHYEKVDIDLRDQVSLQH
                     GAQIFTNYCLSCHSASGMRFNRLKDIGLTDEEIKKNLMFTTDNVGDVMHSAMNPKDAA
                     KWFGAAPPDLTLIARSKGADYLYAYMRGFYKDPTRPSGWNNTVFDKVGMPHPLWEQQG
                     VQAVELDAKGQPVMVKDEHGEMKPKLYWESTGLHSRRLPNGKVIQKEYDAYVRDLVNY
                     LVYMGEPAQLQRKRIGYVVMIFLFAVMLPLAYFLNKEFSKDIH"
     gene            5097..6887
                     /gene="rmuC"
                     /locus_tag="ACFM8S_000899"
     CDS             5097..6887
                     /gene="rmuC"
                     /locus_tag="ACFM8S_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFC6313991.1"
                     /translation="MELMTVLLPLAALVSGVLFTWLLMKSRFQGVLAVLNAQLAEKAA
                     RCDFVEQAHAEIASELAVLDGKYEHLQDENYALGNRFSAAEKQIAYLQEKEAESARLK
                     QSYIELQEKAQGLAVENERLATQLGQERKAFADQYALERQIRQRVETDLEESRQTVRD
                     VQNDLSDVGNRFAAAEKQIAHLQEKEAEAERLRQSHTELQEKAQGLAVENERLATQIE
                     QERLASEEKLSLLGEARKSLSDQFQNLANTILEEKSRRFTEQNREQLHQVLNPLNERI
                     HGFGELVKQTYDKESRERLTLENELKRLQGLNAQLHSEAKALTNALTGTQNKVQGNWG
                     EMILETVLENSGLQKGREYVVQAASVRKEEDGGTRRLQPDVLVNLPDNKQIVIDSKVS
                     LTAYVRYTQAADADTAARELAAHVASIRAHMKGLSLKDYTDLEGVNTLDFVFMFIPVE
                     PAYLLALQNDAGLFQECFDKRIMLVGPSTLLATLRTVADIWRNEQQNQNALAIADEGG
                     KLYDKFVGFVQTLESVGKGIDQAQNSFQTAFKQLAEGRGNLVGRAEKLRLLGVKAGKQ
                     LQRDLVERANEQTAFALGKPSEQEAADEAE"
     gene            complement(6955..7587)
                     /locus_tag="ACFM8S_000900"
     CDS             complement(6955..7587)
                     /locus_tag="ACFM8S_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFC6313992.1"
                     /translation="MTLRYEIFPVTPFRQNCTLIWDDESGEAVLTDVGGDVPFLLQAL
                     ANRKLTLTAIWLTHGHLDHAGGVVEMLKTHKVPVLGSHREDEFLLQSLPQTTAQYGFP
                     VSPAFAPNRWLEEGETLKVGRYAFQVLHIPGHTPGHVVFYCAEAELLIAGDVLFYETI
                     GRTDFPRGNHADLINNIRNKLFALPETVQVVAGHGRMTSIGHEKRHNPFF"
     gene            7679..8503
                     /locus_tag="ACFM8S_000901"
     CDS             7679..8503
                     /locus_tag="ACFM8S_000901"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HFC6313993.1"
                     /translation="MIKKTIGGIIPIFTAVFIPASAGAADLMLAQEYKGQDIAGWAMS
                     EKLDGVRAYWDGKHLISRQGYAFTPPKGFTAQFPPYPLDGELYSGRGQFEQISATVRS
                     VSSDWRGIRLHVFDVPKAQGNLYQRLAVATQWLKTHPNAPITIIPQTKVRDRQHAMDF
                     LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGA
                     IGCKNRHGEFRIGSGFKDKDRDNPPKIGTLITYRYRGFTRKGTPKFATFVRVRTDR"
     gene            8572..9135
                     /locus_tag="ACFM8S_000902"
     CDS             8572..9135
                     /locus_tag="ACFM8S_000902"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HFC6313994.1"
                     /translation="MTDLETKRLETQAMLENADLLFDQGQCRAALQKVADEITRDLGG
                     KYPLLLPVMGGAVVFTGQLLPLLRFPLDFDYVHVSRYGDKLEGGAFNWKRMPDAEQIR
                     GRHVVVLDDILDEGHTMSAIQAKLLEMGAASCRAAVFANKLIDKPKPIRADYVGLDVP
                     NRYVFGYGMDAAGCWRNLGEIYALDGK"
     gene            9216..9653
                     /locus_tag="ACFM8S_000903"
     CDS             9216..9653
                     /locus_tag="ACFM8S_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFC6313995.1"
                     /translation="MIGLLIITHETIGEAYRKLAHHFFPGGLPENVRILGVQPTEDQD
                     DIINNAIAALQEFPDNDGVLIMTDIFGATPCNAARRLVRENKSAILTGLNAPMMVKAV
                     QYSPAAEDLAAFTECVREAAVKGIFAITSAPEDLVCRRSGDAV"
     gene            9722..9991
                     /locus_tag="ACFM8S_000904"
     CDS             9722..9991
                     /locus_tag="ACFM8S_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFC6313996.1"
                     /translation="MLKQSIEIINKLGLHARASNKFTQTASQFKSEVWVTKNDSRVNG
                     KSIMGLMMLAAAKGTVIELETDGADEAEAMRALTDLINGYFGEGE"
     gene            9991..11766
                     /gene="ptsP"
                     /locus_tag="ACFM8S_000905"
     CDS             9991..11766
                     /gene="ptsP"
                     /locus_tag="ACFM8S_000905"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFC6313997.1"
                     /translation="MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAEADTDAEAE
                     RFDAAVKATRKELEQLRSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKIN
                     AEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
                     TVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLIT
                     EGETVIVDGINGVLIIAPDESVLNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIEL
                     VGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYEVYSAIVKKMKGKSV
                     TIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAVH
                     GPVRMMWPMITSVSEVRQCLIHLDTAQRQLAERGDAFGKVGIGCMIEIPSAALTVGSI
                     LKLVDFISVGTNDLIQYILSVDRGDDSVSHLYQPGHPAVLKMLQHVIRTANRMDKDVS
                     VCGEMAGDTAFTRVLLGMGLRRFSMNPNNILPVKNIILHSNVGQLENEIAKIVRCEDE
                     EKAEKLIKQINSVSVEEEAEFKGRK"
BASE COUNT         2966 a         3018 c         3375 g         2738 t
ORIGIN      
        1 tgaaaaaata gaaaaactgc cgggtatggt tgaataaggg gtcagaccgg ttccagttcg
       61 ctcagtccgg gcaaatctgc aaaaccgcgt tcgcgtatga tttggcaaaa attgttcaga
      121 tagctcttgt ccgtatcttc ggtacggatg gcggcataca gtttgctttg cagtccgtcg
      181 tcggtaatct gccggtgaac gacatagcct ttttcaaggt agggcatgac tgtccaatag
      241 ggaagggcgg caatgccacg tctgctggca accagttgga taatggcgat ggtcagttcg
      301 ctgtgtcggc gcggcgggtt gatgtttttc ggaatcagga tttttttggg caaatccagc
      361 atctcgtcgg gaacgggata agtaatcagg gtttccccga taaagtcttc ggcttcccag
      421 acagttttgg cggcaagcgg gtgatctttg gcgcagatgc cgaccatttc gtaggcaaac
      481 agtggttgga agctgatgcc gctttgtttt tccgcttcgg aaacaatggc aaggtcggcg
      541 cggtgttgca gcagcagccc gacgggatcc gcttggaatc ccgatacgat atccagttcg
      601 acttggggcc acatcgggcg gaattcgccc atggcgggca tcagccagtc gaaacaggta
      661 tggcattcga cggcaatccg cagctctccc gcctcccctt ccgtgattcg cgccaaatct
      721 cgttctgcaa cagcaacttg aggtatcagt tcgtgggcga ggcgcagcag cctttcgccc
      781 accggggtaa agcgcaaggg cgtggatttt cgttcgaaca acggtgcgcc gtagtggttt
      841 tcaagcatac ggatttggtg ggaaagtgca gattgggtaa ggaaaacccg tttggcggca
      901 agggagacgc tgccggtttc ttcaagtgcc agcagggtct tgaggtggcg cagttcgatg
      961 atggaatcca tggggcttca gacggcatat tgaaccggcg catattaaaa taattcatat
     1021 gcgggtgcaa atcggaaaaa tgtgaattcc ggacattttg cgattagaat gcccgcttgt
     1081 ttaaagcgat taggaaaaaa gatggtattg cgcagggatt ttctgacttg gtgtaatgaa
     1141 acattgcaga cagcgttgtt taaagattac gcccctaacg gtttgcaggt tgaagggagg
     1201 gaatatatcg ggaaaatcgt tacgtcggta acggcaagca gggcagcgat tgattttgct
     1261 gtggagcaga aggcagattt gcttttggta catcacggta tgttctggaa aaacgagttg
     1321 ccgaccgtta ctggttggaa aaaagaacgg attgccgcac tgttacggca cgacatcaat
     1381 atggcaggct accatctgcc cctggatgca catcccacac tgggcaacaa tgcccaactc
     1441 gccgacagat tgggttttgc gacagaaaaa cggttcggcg aacaaaacct gctcaactcg
     1501 ggcagcctga aacaagccaa gacactcggc gcattggcgg cgcatattga aacagttttg
     1561 caacgtaagc ctgtcgtcat cggcaaaccc gaacgcgaaa tccgacgggt tgcatggtgt
     1621 agcggcgggg cgcaggggtt ttttcagacg gcaatagacg aaggtgtcga tctgtatttg
     1681 acgggggaaa tctctgaagc tcaataccac cttgccaatg aaacgggtac ggctttcatt
     1741 tcggcagggc atcacgcgac ggaacgttac ggcgtacgcg cgctggcaga atcggcggca
     1801 gaggttttcg ggttggaagt gtgccatttt gacgaaaaca atccggcttg agtcttgaga
     1861 aatatcataa aactttacct tattttaaat aatgctttga gtatcactgc aaactgggta
     1921 aaattgcaac gtttaatggc tcggtattcc caaaatatta ggacgactga ataatggata
     1981 atcaagaaat caacaacggc cgccgccgtt tcctgacact cgcgacctgc ggcgcgggcg
     2041 gagtggcagc attgggtgtg gcaacgccgt ttgtggccag ttttttccct tcggaaaaag
     2101 ccaaggccgc cggtgctgcc gtcgaggtgg atgtcagtaa aatcgaagcg ggtcagctgc
     2161 tgaccgccga gtggcaaggc aaaccgattt gggtgctcaa ccgtacagat cagcagctta
     2221 aagacctgaa aggcctgaac ggcgaactta ccgatcccaa ttccgatgcg gaacagcagc
     2281 cggagtatgc taaaaacgag acccgttcga ttaagccgaa catccttgtc gccatcggta
     2341 tctgcaccca tttgggctgc tcgcccacct tccgtcccga cattgccccc gccgatttgg
     2401 gtgcagactg gaaaggcggc ttcttctgcc cctgccacgg ttcgaaattc gacttggccg
     2461 gccgcgtata taaaggtgtt cctgccccga ccaacctggt tgtcccgcca tataaatact
     2521 tgagcgacac aactatcttg gtgggcgaag actaagaata aggaacgata attatggcaa
     2581 accaaaccaa tagcaaagca aaagcattgt taggctgggt agatgcccgt tttccattaa
     2641 gtaaaatgtg gaaagagcat ctgtctgaat actatgcgcc taaaaacttc aacttctggt
     2701 atttcttcgg ctcattgtct atgctggtgc tggtgattca aatcgtcagc ggtattttcc
     2761 tgaccatgaa ctacaaaccg gacggcaacc ttaacgccta ccatctgcct gctgccttta
     2821 ccgcagtaga gtacatcatg cgcgacgtgt ccggcggctg gattatccgc tatatgcact
     2881 ctaccggcgc atctttcttc ttcatcgtcg tttatctgca catgttccgt ggtctgattt
     2941 acggttcgta caaaaaaccg cgcgaattgg tgtggatttt cggttccctg attttcttgg
     3001 cattgatggc agaagccttt atgggttacc tgttgccttg gggtcaaatg tccttctggg
     3061 gtgcgcaggt aattattaac ctgttctccg ccatccctgt tatcggtcct gatttgtcca
     3121 cttggatccg cggtgacttc aacgtttccg atgttacttt gaaccgattc ttcgccctgc
     3181 acgttatcgc tgtacctttg gtattgctcg gcttggttgt ggctcatatc attgccttgc
     3241 atgaagtggg ttccaacaac cctgacggtg tagaaatcaa aaagctgaaa gatgaaaacg
     3301 gtgtccctct agatggcata cctttttttc cgtattatgt tgtgcatgat atattggcag
     3361 taacgatatt cttgattgtc ttctgtgccg tgatgttctt tgcaccagaa ggcggcggct
     3421 acttcctcga agcgccgaac ttcgatgctg cgaatgcgct gaaaacacct ccgcacattg
     3481 caccggtatg gtacttcact ccgttctacg ccattttgcg tgcgattccc tcgttcttgg
     3541 gaacacaggt atggggtgta atcggtatgg gtgcggcagt tgtactgatt gccctgttgc
     3601 cttggttgga taaaggcgag gttaaatctg tccgctatcg cggcccaatc ttcaaaaccg
     3661 cattggttct gttcatcatt gccttcatcg gtttgggtat tttgggtgca atggtagcaa
     3721 ctgatactcg tactttggtt gcacgtatcc tgtctttcgt ctactttgca ttcttcctgg
     3781 gtatgccgtt ctataccaaa ctggatacca acaaaccagt tcctgaacgc gtaaccatga
     3841 gcactactaa acaaaaaatt atgttctttg tttacgtcgg tattaccgtt gttggtgctt
     3901 acttgtttgc aaccaatatc tgatgagggc agcgaaaatg aaacaaactc tgaaaaactg
     3961 gtttgctgcc ttattgctgg cagtgcctat gagtgcagcc gtcgccagcg gcggcggaca
     4021 ctacgaaaaa gtcgatatcg acctgcgtga ccaagtcagc ctgcagcacg gtgcgcaaat
     4081 ctttacaaac tactgtttgt cttgccactc tgcaagcggt atgcgcttca accgtctgaa
     4141 agacatcggt ttgactgacg aagaaatcaa gaaaaacctg atgtttacca ccgataatgt
     4201 cggcgatgtc atgcattcgg cgatgaaccc gaaagatgcg gcaaaatggt ttggtgctgc
     4261 tccgcccgat ttgacgttga ttgcgcgttc caaaggtgca gactaccttt acgcctatat
     4321 gcgcggcttc tataaagatc cgacccgtcc gagcggctgg aacaatactg tattcgataa
     4381 agtcggtatg ccccacccgt tgtgggagca acaaggtgtt caagccgttg agttggatgc
     4441 caaaggtcag ccggttatgg taaaagacga acacggcgag atgaagccta agctgtattg
     4501 ggaatctacc ggtttgcaca gccgccgcct gcctaacggc aaagtgatcc aaaaagagta
     4561 cgacgcatat gtacgcgatt tggtcaatta ccttgtgtac atgggcgaac ctgcacaact
     4621 gcaacgcaaa cgtataggct atgtcgtgat gattttccta tttgcggtta tgctgccttt
     4681 ggcttacttc ctaaataaag aattttctaa agatattcat taatcattaa aaaagaatat
     4741 tggaaagacg tacactaagc gtttggaaca aaagggcaaa tcctttaggg tttgcccttt
     4801 tttcattttg cctgccgttt gaaaagcctg aatccgtatg ccgtctgaac atagctgcaa
     4861 catttcagac ggcatcattc taaaaatgtc agacatcggg aacttaatca gggtttgtgg
     4921 cagattgata ttgaattagg aaaaggtctg aaaccgcaag cagcatggta aggcgatgtc
     4981 gaacaggttt aaagccaatc cgctatattt tttggagcta tgtcgaacaa acaggtagaa
     5041 tcggaataag cggcggtttt gtccggtgtg aaaatgtttg aatattagga tgattgatgg
     5101 aactgatgac tgttttgctg cctttggcgg cgttggtgtc gggcgtgttg tttacatggt
     5161 tactgatgaa gagccggttt caaggtgtat tagccgtatt gaacgcgcaa ttggcggaaa
     5221 aggcggcaag atgtgatttt gttgaacagg cacacgcaga aattgcatcg gaattggccg
     5281 ttttggatgg gaaatacgag catttgcagg acgaaaatta tgctttgggc aaccgttttt
     5341 ccgcagccga aaagcagatt gcttatttgc aggaaaaaga ggcggagtcg gcgcgtctga
     5401 agcagtcgta tatcgagttg caggaaaagg cacagggttt ggcggttgaa aacgaacgtt
     5461 tggcaacgca gctcggacag gagcggaagg catttgccga ccaatatgcc ctggaacgcc
     5521 aaatccgcca aagagtcgaa accgatttgg aagaaagccg ccaaactgtc cgcgacgtgc
     5581 aaaacgacct ttccgatgtg ggcaaccgtt ttgccgcagc cgaaaaacag attgcccatt
     5641 tgcaggaaaa agaggcggaa gcggagcggt tgaggcagtc gcataccgag ttgcaggaaa
     5701 aggcacaggg tttggcggtt gaaaacgaac gtttggcaac gcaaatcgaa caggaacgcc
     5761 ttgcttctga agagaagctg tccttgctgg gcgaggcgcg caagagtttg agcgatcagt
     5821 ttcaaaatct tgctaacacg attttggaag aaaaaagccg ccgttttacc gagcagaacc
     5881 gggagcagct ccatcaggtt ttgaacccgc tgaacgaacg catccacggt ttcggcgagt
     5941 tggtcaagca aacctatgat aaagaatcgc gcgagcggct gacgttggaa aacgaattga
     6001 aacggcttca aggattgaat gcgcagctgc acagcgaggc aaaggcactg accaacgcgc
     6061 tgaccggtac gcagaacaag gttcagggca attggggcga aatgattctg gaaacggttt
     6121 tggaaaattc cggcctccag aaagggcgcg aatatgtggt tcaggcggca tccgtccgaa
     6181 aagaggaaga cggcggcacg cgccgcctcc agcccgacgt tttggtcaac ctgcccgaca
     6241 acaagcagat tgtgattgat tccaaggtct cgctgacagc ttatgtgcgc tacacgcagg
     6301 cggcggatgc ggatacggcg gcacgcgaac tggcggcaca cgttgccagc atccgtgcac
     6361 acatgaaagg cttgtcgctg aaggattaca ccgatttgga aggtgtgaac acattggatt
     6421 tcgtctttat gtttatccct gtcgaaccgg cctacctgtt ggcgttgcag aatgacgctg
     6481 gcttgttcca agagtgtttc gacaaacgga ttatgctggt cggccccagt acgctgctgg
     6541 cgactttgcg gacggtggcg gatatttggc gcaacgagca gcaaaaccaa aacgcgctgg
     6601 cgattgcgga cgaaggcggc aagctgtacg acaaatttgt cggctttgta cagacgctcg
     6661 aaagcgtcgg caaaggcatc gatcaggcgc aaaacagctt tcagacggca ttcaagcaac
     6721 ttgccgaggg gcgcgggaat ctggtcggcc gcgccgagaa actgcgtctg ttgggcgtga
     6781 aggcaggcaa acagcttcaa cgggatttgg tcgagcgtgc caacgaacag acggcatttg
     6841 ctttgggcaa accttcggaa caggaagcgg ctgacgaagc cgaataggaa aggcggatgc
     6901 gtttcagacg gcatcagtgc agatgacgcc gtctgaagac cgtagtgaag tcggttagaa
     6961 aaacggattg tgccgttttt cgtgtccgat ggaagtcata cgcccgtgtc cggcgacaac
     7021 ttgcacggtt tcggggaggg cgaataattt gttgcggata ttattgatta agtcggcgtg
     7081 gttgccgcgc ggaaaatcgg ttctgcctat ggtttcgtaa aacagcacgt cgcccgcaat
     7141 cagcagctcc gcttcggcac aatagaaaac cacgtgtccc ggcgtgtgcc ccggaatatg
     7201 cagcacttga aaggcatagc gtccgacttt gagcgtttcg ccttcttcga gccaacggtt
     7261 cggcgcaaag gcgggcgaga cgggaaatcc gtattgcgcg gtggtttgcg gcagcgattg
     7321 gagcaggaat tcatcttcgc ggtgcgaacc gaggacaggg actttatgcg ttttcaacat
     7381 ttcgaccacg ccgcccgcgt gatcgagatg gccgtgcgtc agccagattg ccgtgagcgt
     7441 aagtttgcgg tttgccaacg cttgcagcag gaacggcacg tcgccgccga catcggtcag
     7501 gacggcttcg ccgctttcgt cgtcccaaat cagggtgcag ttttggcgga agggggtaac
     7561 ggggaagatt tcgtaacgta gggtcatcgg cgtgttccta aacggttttt cagacggcat
     7621 cgggtttgcc gtttgtttta tggcggtttg ccgcctgttt tgatattggg ggaatcagat
     7681 gattaagaag acaatcggcg gcatcatacc gatttttacg gcggttttca tccctgcatc
     7741 ggcaggcgcg gcggatttga tgctggcgca ggaatacaaa gggcaggata ttgccggctg
     7801 ggcaatgagc gagaaactcg acggcgtgcg cgcctattgg gacggaaagc acctgataag
     7861 ccgtcagggc tacgcgttta cgccccccaa aggctttacc gcgcagtttc cgccttatcc
     7921 tttggacggc gaattgtata gcggacgtgg tcagttcgag cagatttccg ctaccgtgcg
     7981 ttctgtttct tcagactggc gcggcatccg cctgcacgtt ttcgatgtac ccaaggcgca
     8041 gggcaatctc taccaacgtt tggcagtcgc aacgcagtgg ctgaaaacgc atccgaacgc
     8101 gccgattacc atcatcccgc aaaccaaagt gcgcgaccgg cagcacgcga tggacttttt
     8161 aaaacaaatc gaagcgcagg gcggcgaagg cgtgatgctg cgtcagcccg aatcccgtta
     8221 cagcggcggc aggagcagcc aattattgaa gctgaaaagc caatacgacg acgaatgcac
     8281 ggtaacgcgg cactatgagg gcaaagggcg aaacgccgga cggttgggcg cgatcggctg
     8341 caaaaaccgg cacggcgaat tccgcatcgg cagcggtttc aaagacaaag accgcgacaa
     8401 cccgcccaaa atcggcacac tgattaccta ccgctaccgt ggttttacgc ggaaaggcac
     8461 gccgaaattt gccacatttg tgcgcgtgcg taccgaccgc tgagcaggac ggttcagacg
     8521 gcagccgata ctttggttta taattttcct tttaccgacc gattccgaca tatgaccgat
     8581 ttagaaacca aacgccttga aacacaggcg atgcttgaaa acgccgatct tttgttcgac
     8641 caaggccaat gccgtgccgc actgcaaaaa gtggcggacg agattacgcg cgatttgggc
     8701 ggcaaatatc cgctgctgct gcccgtgatg ggcggcgcgg tggtgtttac ggggcagttg
     8761 ctgccgctgt tgcgttttcc cttagatttt gattatgttc acgtttcccg ttacggcgac
     8821 aagctggagg gcggcgcgtt caactggaag cgtatgcccg atgcggaaca aatccggggc
     8881 aggcacgtcg tcgtgctgga cgatattttg gacgaagggc atacgatgtc cgccattcaa
     8941 gccaaacttt tggaaatggg tgcggcaagc tgccgtgcgg cggtgttcgc caacaagctg
     9001 attgacaaac ccaagcctat ccgagccgat tatgtcggac tggatgtgcc gaaccgttat
     9061 gttttcggtt acggcatgga tgcggcgggc tgctggcgca atttgggcga gatttacgca
     9121 ttggacggaa aataaggacg cgatgccgtc tgaaggctgt tcagacggca ttgcgcccat
     9181 acgccggcag gacaacgagg aacaggacgc ataatatgat agggctttta atcatcacac
     9241 acgaaaccat aggcgaagcc taccgcaagc tggcacatca tttttttccg ggcggactgc
     9301 ctgaaaacgt ccgcatactc ggcgtgcagc cgacggaaga ccaagacgac atcatcaaca
     9361 acgccattgc cgcgcttcag gagtttcccg acaacgacgg cgtgctaatt atgaccgata
     9421 ttttcggcgc gaccccctgc aatgcagccc gccgcctcgt gcgcgaaaac aaatcggcga
     9481 ttttgaccgg gctgaacgcg ccgatgatgg ttaaggccgt ccaatattcg ccggcggcgg
     9541 aagaccttgc cgcctttacc gaatgcgtca gggaggcggc tgtaaaaggc attttcgcca
     9601 tcacgtccgc gcccgaagat ttggtgtgcc ggcgcagcgg cgatgccgtc tgaagaagcg
     9661 gcagggcagg aaaacatttt aaacggcgtc tgctgccgat atacataaca cgggaaccga
     9721 aatgctcaaa caatccatcg aaatcatcaa caaactcgga ctccacgccc gcgcgtccaa
     9781 caagttcacc caaaccgcgt cccaattcaa aagcgaagtc tgggttacga aaaacgacag
     9841 ccgcgtcaac ggcaaaagca ttatggggct gatgatgctc gccgccgcca agggtacggt
     9901 catcgaactg gagacggacg gcgcggacga ggcggaagcg atgcgcgccc tgaccgactt
     9961 aatcaacggc tacttcggcg agggcgaata atgagtatcg tgctgcacgg cgtggcggcg
    10021 ggcaaaggca ttgccgtcgg ttgcgcccac ctgattgcgc gcggtacgga ggaagtgccg
    10081 cagtatgatg ttgcggaggc ggacaccgat gccgaagccg aacgtttcga tgccgccgtc
    10141 aaagccacgc gcaaagagtt ggaacagctc cgcagcgcga ttcccgaaaa cgccccgacc
    10201 gagttgggcg cgttcatctc gctgcacctg atgctcttga ccgatgttac cttgtcgcgc
    10261 gaacccgtcg atattttaag ggaacaaaaa atcaacgccg agtgggcatt gaagcagcag
    10321 agcgacaaac tcgccgccca attcgacaat atggacgatg cctatttgcg cgaacgcaag
    10381 caggatatgc tgcaagtcgt ccgccgcatc cacaacaacc tgatcgggca gggcaacgag
    10441 ttggaagttg ccgacaacct gtttgacgaa accgttctga ttgcaaacga cctttcgccc
    10501 gccgacacgg ttttgtttaa agagcagcgc attgccgcct tcgttaccga tgccggcggc
    10561 cccaccgggc atacggcgat tttgggcagg agcttggaca tcccgtccgt cgtcgggctg
    10621 cacaacgcgc gcaaactgat taccgaaggc gaaacggtca ttgtggacgg tatcaacggc
    10681 gtgttgatta tcgcgccgga tgagtcggtg ttgaacgaat accgccgccg tgcccgcgaa
    10741 taccgcagcc acaaacgcga tttgaacaag ctcaaaaaaa ccgccgccgc caccgccgac
    10801 ggggtctgca tcgagcttgt gggcaatata gaatccgccg aagacgtgaa accgctgcac
    10861 aacctcggcg cagacggcat cgggctgttc cgcagcgagt ttctttacct gaaccgcgat
    10921 acgatgccgt ctgaagacga gcagtacgaa gtgtacagcg cgattgtcaa aaaaatgaaa
    10981 ggcaaaagcg taacgatacg gacagtcgat ttgggtgtgg acaaaaaccc gcgctggttc
    11041 gggaaaaaca gcacgcccaa cggcagcctc aaccccgcgc tgggcatgac cggcatccgc
    11101 ctgtgccttg ccgaaccggt catgttccgc acccagatgc gcgccatcct ccgtgcggcg
    11161 gtacacggcc ccgtgcggat gatgtggccg atgattacct cagtatccga agtgcgccag
    11221 tgcctcatcc acctcgacac cgcgcaacgc cagcttgccg aacgcggcga tgccttcggt
    11281 aaagtcggca tcggctgtat gattgaaatt ccgtctgccg cgctgaccgt cggcagtatt
    11341 ttgaaactgg tcgatttcat ctccgtcggt accaacgacc tgattcaata catcttgtcc
    11401 gtcgatcgcg gcgacgacag cgtcagccac ctctaccagc ccggccatcc cgccgtgctg
    11461 aaaatgctgc aacacgtcat ccgtaccgcc aaccgcatgg acaaagacgt atccgtatgc
    11521 ggcgagatgg cgggcgatac cgcgtttacc cgcgttttat tgggtatggg gctgcgccgt
    11581 ttttccatga accccaacaa catcctgccc gtcaaaaaca tcattctgca cagcaatgtc
    11641 ggacagctcg aaaacgagat tgccaaaatc gtccgttgcg aggacgagga aaaggcggaa
    11701 aagctgatca aacagatcaa cagcgtgtct gtcgaggaag aagccgaatt caaggggcgg
    11761 aaataaatac ggcaggtaaa aaatagaaat acttaacaat gcccgcaatc tgaaattttg
    11821 ccattcttgc aaaatagaaa accgaaacag aaacccaaaa tcggtcattc cctcaaaaac
    11881 agaaaaccaa aatcagaaac ctaaaacccc gtcattcccg cgcaggcggg aatctaggtt
    11941 tgtcggcaca gaaacttgtc gggaaaaacg gtttctttag attttacgtt ctagattccc
    12001 gcctgcgcgg gaatgacgat gaaaagattg ttgtcgcttc ggataaattt ttgccgtgtt
    12061 gggttctaga ttcccgcttt tgcgggaatg acggcag
//
LOCUS       DAXHSF010000043        11967 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.043, whole genome shotgun sequence.
ACCESSION   DAXHSF010000043 DAXHSF010000000
VERSION     DAXHSF010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11967
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.043"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            141..575
                     /locus_tag="ACFM8S_000906"
     CDS             141..575
                     /locus_tag="ACFM8S_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HFC6313998.1"
                     /translation="MNFALSVIMLTLASFLPVPPAGAAVFTWKDGGGNSYSDVPKQLH
                     PDQSQILNLRTRQTKPAVKPAQADAGKRTDGAAQENNPDTAEKNRQLEEEKKRIAETE
                     RQNKEENCRISKMNLKAVGNSNAKNKDDLIRKYNNAVNKYCR"
     gene            complement(623..1966)
                     /locus_tag="ACFM8S_000907"
     CDS             complement(623..1966)
                     /locus_tag="ACFM8S_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFC6313999.1"
                     /translation="MFHSVNVFTGETLYRRPAQDYAEFERRLADLKMRGGAFAQLGVT
                     ERAARLQKFADRLEAEKERFAEMVCEEVGRCLHECRAEIGKSIELIRYYARLAPELLA
                     HKTIATQASLSQVRFEPLGVVFAVMPWNYPVWQVLRFAVPAMCAGNACAVKPAPSVAR
                     VSQALFDLASDGIPLAGVWLDEAGTLKAVEDTDAMAFTGSTHTGRILAAHAGANLKKT
                     VLELGGSNAFIVMPDADLERAAAEACYSRFRDAGQSCNAAKRIIVTEAAADRFITLFL
                     AECAKLKMGDPKHPDTTLAPLHREDLRDRVHGQVEDAVSNGAVCLTGGKIPQGRGWFY
                     PATVLDRVNPACRVWREEVFGPAALILRAENEEHAIALANDSPFGLGACIYTADTERA
                     WRFAEKIQAGSVFINRHTSSDLRLPFGGVKDSGYGRELSEFGLYEFVNVKTYWQK"
     gene            complement(1971..2624)
                     /locus_tag="ACFM8S_000908"
     CDS             complement(1971..2624)
                     /locus_tag="ACFM8S_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HFC6314000.1"
                     /translation="MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHSTKERRKVAR
                     TAAVAVFAVIAVFALIGGTLLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNL
                     GAQPETGQARPARNAGAIAVVPIAIPITIGPGGISTVIIYASAAKTYGDIALIIAAGL
                     VVSAICYAILIVAGKVSRLLGATGLTILNRIMGMMLAAVSVEIIVSGLKTIFPQLAG"
     gene            2775..3065
                     /gene="gatC"
                     /locus_tag="ACFM8S_000909"
     CDS             2775..3065
                     /gene="gatC"
                     /locus_tag="ACFM8S_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFC6314001.1"
                     /translation="MALTLADVDKIARLSRLHLTAEEKEKSLQELNDIFTMVEQMQNI
                     NTDGIEPMAHPHEAALRLREDEVTETDRAAEYQAVAPEVRDRLYIVPQVIEE"
     gene            3128..4573
                     /gene="gatA"
                     /locus_tag="ACFM8S_000910"
     CDS             3128..4573
                     /gene="gatA"
                     /locus_tag="ACFM8S_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFC6314002.1"
                     /translation="MTQYTLKQASVLLQSKQISAVELASAYLAAIAEKNPALNGYITI
                     DQDKTLAEARAADERIAQGNASALTGVPVAYKDIFCQTGWRSACASKMLDNFISPYTA
                     TVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGAAKNPWNLEHVPGGSSGGSAAVVA
                     ARLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQTGPMAQTAE
                     DCAILLNAMAGFDPKDSTSLEREKEDYTRDLNQPLKGLKIGLPKEYFGEGNSADVLTA
                     LQNTIDLLKAQGAELIEVSLPQTKLSIPAYYVLASAEASTNLSRYDGVRYGHRAAQFA
                     DLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFAR
                     CDLILAPTAPTAAPKIGADASPVETYLSDIYTIAVNLAGLPALTLPAGFSGGGLPVGV
                     QLVGNYFAEAKILGAAHQIQLNSDWHGKRPE"
     gene            4570..5502
                     /locus_tag="ACFM8S_000911"
     CDS             4570..5502
                     /locus_tag="ACFM8S_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFC6314003.1"
                     /translation="MKQNRTFTFPDFRTVYSYAPLYRLQHLKYTLRKFFGKKEIYAFE
                     QFVNASPIRQGLFLHCPQNAYPLLREFVDRRFNCKRRLDAMTADFLMAEKLFGTDILH
                     QMEDYRFHLVLAHLSDGISLWLNRNDNCVEEGAWSLSLRDEAGNRLYMATFAFVGTHL
                     LTASVQGPAGEEAKDTVRRITKQLHGLRPQQLMVTALQYFAAVLGLDGAMGIAQKHQV
                     KLRWKLKKRVKMNYDAFWQEYGASLERDGYWHLPQTPARKDLTDIESKKRSMYRKRYE
                     MLDNMVAEMKDGLKTEARGISDGIQTEKPLRRTA"
     gene            5545..6975
                     /gene="gatB"
                     /locus_tag="ACFM8S_000912"
     CDS             5545..6975
                     /gene="gatB"
                     /locus_tag="ACFM8S_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFC6314004.1"
                     /translation="MTWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHAGVVECAL
                     PGVLPVMNREVVEKAIKLGLALDAKINRKNVFDRKNYFYPDLPKGYQISQLDLPIVEH
                     GKLEIVVGDDVKTINVTRAHMEEDAGKSVHEGLNGATGIDLNRAGTPLLEVVSEPEMR
                     SAAEAVAYAKALHSLVTWLNICDGNMAEGSFRVDANVSVRPKGQAEFGTRREIKNLNS
                     FRFLEQAINYEAEAQIEILEDGGKVQQATMLFDPEKGETRVMRLKEDAQDYRYFPDPD
                     LLPVIISDAQMQKAKAEMPELPKEMAVRFVADYGVSEYDARLLTASRAQAAYFEEAAK
                     ESGQGKPTANWMNGELAAALNKEGMELADSPITAPRLAALVGKIADGTLSSKLAKKAF
                     EAMWAEPEATIAEIIEKHGLQQMTDTGAVEAMVDEVLANNAKAVEQFKSGNEKALNAI
                     VGQVMKASKGKANPAQVQELIKAKLA"
     gene            7067..8077
                     /locus_tag="ACFM8S_000913"
     CDS             7067..8077
                     /locus_tag="ACFM8S_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HFC6314005.1"
                     /translation="MNHTVTLPDQTTFAAGDGETVLAAAARQNLNLPHSCKSGVCGQC
                     KAELVSGDIQIGGHSEQALSEAEKAQGKILMCCTTAQSDISLNIPGYKADALPVRTLP
                     ARIESMVFKHDVALLKLALPKAPPFAFYAGQYIDLLLPGNVSRSYSIANSPDQEGILE
                     LHIRRRENGVCSEMIFGSEPKVKEKGIVRVKGPLGSFTLQEDSGKPVILLATGTGYAP
                     IRSILLDLIRQDSGRAVHFYWGARHQDDLYALEEAQGLACRLKNACFTPVLSRPGEGW
                     QGRKGHVQDIAAQDHPDLSEYKVFACGSPTMTEQAKNLFVQQHKLPENLFFSDAFTPS
                     AS"
     gene            complement(8206..8838)
                     /gene="pdxH"
                     /locus_tag="ACFM8S_000914"
     CDS             complement(8206..8838)
                     /gene="pdxH"
                     /locus_tag="ACFM8S_000914"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFC6314006.1"
                     /translation="MDLHNIREDYSKRELSEGDCADNPIEQFERWLDEAVRAQVNEPT
                     AVNVAAVDGRGRPNSRMVLLKEVNSEGFVFFTNYHSRKGRSLDAHPFAAMTFFWPELE
                     RQVRVEGRVERLAEKLSDEYFESRPYQSRLGAWASAQSEVIPNKAVLVAKAAAVGLKH
                     PLHVPRPSHWGGYIVIPDLLEFWQGRPSRLHDRIQYRLLDGGWIRERLSP"
     gene            8898..9050
                     /locus_tag="ACFM8S_000915"
     CDS             8898..9050
                     /locus_tag="ACFM8S_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020424398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314007.1"
                     /translation="MHKLCPQSRSDDFIRKTAASGTISNRPTEGGILSTRPAAHAAEE
                     PPYQAS"
     gene            9059..10108
                     /locus_tag="ACFM8S_000916"
     CDS             9059..10108
                     /locus_tag="ACFM8S_000916"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFC6314008.1"
                     /translation="MSKQPTSKRQWRDGAAPSAKKTAKPFKSKAHPKGETRKTASQPY
                     GQKASDGIKPQNLPKQRAAKAKKLVVRNPNQKIMEHARDLKERRSDLSRMEPERLQKV
                     LAASGVGSRREMEEWINNGWVTVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRI
                     ILYYKQEGEIVSRDDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQ
                     RFAHPSFEVEREYAVRVLGGLTGEQMRVLTEEGVMLEDGLAKVERIREQGGEGANKWY
                     NVVIKEGRNREVRRIFESQGLTVSRLVRVGFGPIGLPNRLKRGQFYELNPAEVANIIK
                     WADMLLPGERRRKKA"
     gene            complement(10177..11718)
                     /locus_tag="ACFM8S_000917"
     CDS             complement(10177..11718)
                     /locus_tag="ACFM8S_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HFC6314009.1"
                     /translation="MKFLDREASIAKPGFNRWLVPPAALAVHLAIGQIYAYSVFNAPL
                     TKLIGITESAAGDWKLTTVGWIFSIALAMLGASAALFGTWMERVGPRKAIFAAACCFS
                     LGFFVSAFGVRTHNLFLLYLGNGVIGGVGLGLGYIGPVSTLMKWFPDKPGMATGLAIM
                     GFGGGAMLASPLSVSLMNAFSNAASVGVAETFAVLGLLYLALMMFGAFTIRVPADGWK
                     PEGYTAPKTQNKLVSSNHVNVSQAMKTPQFWLLFWVLCLNVTAGIGVLGQASVMIQEL
                     FSETSIGRQAAVGAGAAAGFVSLLSLFNMGGRFLWSSVSDKIGRKNTYTIFFVLGSLL
                     YFAVPSIGEGGSKALFIIGFCVIISMYGGGFAAIPAYLKDLFGTYQVGAIHGRILLAW
                     STAAVIGPVLVNYIRQSQIDSGIPAAQAYSVTMYIMACLLIIGLLCNLAVKSVHEKHH
                     EKDIKTAAHSGNPDDETAISDAYLVGEKVSGGGISVWWRWALAVIPLAYGVVMVFVKA
                     LDLFS"
BASE COUNT         3070 a         3639 c         2978 g         2280 t
ORIGIN      
        1 tttgggaatc tgttttaccc caatatataa agcaccatat taaggcggag tgtcttcccc
       61 actttgaccc gaacccggaa aagacaccgc ccaagccaat cctgatgctg ccccgacagc
      121 caaccattaa ggaaatccta atgaactttg ctttatccgt cattatgttg accctcgcct
      181 ctttcctgcc cgtcccgcct gccggagccg ccgtctttac ttggaaggac ggcggcggca
      241 acagctattc ggatgtaccg aaacagcttc atcccgacca aagccaaatc ttaaacctgc
      301 ggacgcgcca aaccaaaccg gcggtcaaac ccgcccaagc cgacgcaggg aagcgcacag
      361 acggcgcggc acaggaaaac aatcccgaca ctgccgagaa aaaccggcag cttgaggaag
      421 aaaagaaaag aattgccgaa accgaacggc agaacaaaga agaaaactgc cggatttcaa
      481 aaatgaacct gaaggcggtg ggaaattcaa atgcaaaaaa caaggatgat ttgattcgga
      541 aatacaataa cgccgtaaac aaatactgcc gttaatcggc tctagcgcaa acccgatgcc
      601 gtctgaagcg gcacggggtt tgtcatttct gccagtaggt tttgacgttg acgaactcgt
      661 acagcccgaa ttcggacaat tcgcgcccgt aaccggaatc tttgactccg ccgaaaggca
      721 ggcgcaaatc gctgctggta tggcggttga taaacaccga tcccgcctgt attttttcgg
      781 caaaccgcca agcgcgttcg gtatcggcgg tataaatgca ggcaccgagc ccgaacgggg
      841 aatcattggc aagggcgatg gcatgttctt cgttttcggc gcgcaaaatc agggcggccg
      901 gcccgaatac ttcttctctc cagacgcggc aggcaggatt taccctgtct aaaaccgtcg
      961 cgggataaaa ccagcctcgc ccttgtggga tttttccgcc ggtcaggcat accgcgccgt
     1021 ttgaaacggc atcttcaacc tgcccgtgaa ccctgtcccg caaatcttcg cggtgcagcg
     1081 gtgcaagcgt agtatcggga tgtttggggt cgcccatttt caatttagcg cattcggcaa
     1141 gaaacagcgt gataaaacga tcggctgcgg cttcggttac gatgatgcgc ttggcggcgt
     1201 tacacgattg ccccgcatcg cggaaacggg aataacaggc ttctgcggcg gcacgctcca
     1261 aatcagcatc gggcatcacg ataaaggcgt tgctaccgcc gagctccaac acggttttct
     1321 taaggtttgc gcccgcgtgt gccgcaagga tgcgccccgt atgcgttgaa ccggtaaacg
     1381 ccattgcatc ggtatcttca accgccttga gcgtgcccgc ctcatccagc cacacgcctg
     1441 ccagaggaat gccgtctgaa gccaaatcga acagtgcctg actgacgcgt gccacgctgg
     1501 gcgcgggttt gacggcgcac gcgttgcccg cgcacatagc gggaacggcg aaacgcaata
     1561 cctgccagac gggatagttc caaggcatga cggcaaacac cacgcccaaa ggctcgaagc
     1621 gcacctgact caaactcgcc tgcgtcgcga tggttttgtg ggcaagcagt tcgggggcaa
     1681 ggcgggcgta atagcgtatc agttcgatag acttgccgat ttccgcacgg cattcgtgca
     1741 agcagcgtcc gacttcctca cacaccattt ccgcaaaacg ctctttctcc gcctccaaac
     1801 ggtcggcaaa tttttgcagg cgcgcggcac gttcggttac gcccagttgc gcgaacgccc
     1861 cgccgcgcat tttcaaatcc gccagccgcc gttcaaactc cgcataatct tgagctggac
     1921 ggcggtaaag cgtttcgccc gtaaatacat tgacactgtg aaacatcgaa tcaacctgcc
     1981 agttgcggga atatcgtttt cagtcccgac acaataatct ccaccgatac cgccgccagc
     2041 atcataccca taatgcggtt taaaatcgtc agccccgtcg cgcccagcag gcggctgacc
     2101 ttcccggcaa cgattaaaat ggcataacaa atcgcactga ccaccaaacc ggccgcgata
     2161 atcaacgcga tgtcgccgta tgttttagcc gccgaagcgt aaataatcac ggtcgaaata
     2221 ccgcccgggc cgatggtgat cggtatggcg atgggcacga cggcaatcgc tccggcattg
     2281 cgggcggggc gcgcctgccc cgtttccggc tgcgcgccga gattctgctt ggcgggattg
     2341 tcgttgccgt tcatcataga aatggcaatc agcaacacca aaattccgcc gccgacctga
     2401 aacgaaccga cgctgatgcc caaaaccttc agcagcgtac cgccgatcag cgcaaatacc
     2461 gcaatcacgg caaacacggc aacggcggcc gtccgcgcga ccttcctgcg ctccttcgtg
     2521 ctgtgcccgt tggtcaggtc aaggtaaagc gacaacgcgc taaacggatt gatcagcacc
     2581 aaaaaagcca caatcagctt gccgatttcc atgcccaatc ccattatttc cccctccttc
     2641 aaacccgtgc ggcaggcatc cgatgctgca aattgccgcc gcaacggatt tttccgttat
     2701 aattaaaaat tcaagcaata cgccccatca tacccgaacg acggtatctt taccatcaga
     2761 caaggatgct tttcatggca ctgacacttg ccgacgtaga caaaatcgcc cgactctccc
     2821 gactgcacct gactgcggaa gaaaaagaaa aatcgcttca agaattaaac gacattttca
     2881 ctatggtcga acagatgcag aacatcaaca cagacggcat cgaaccgatg gcgcacccgc
     2941 acgaggccgc cctgcgcctg cgcgaagacg aagtaaccga aaccgaccgc gccgccgaat
     3001 atcaggcggt cgcaccggaa gtacgcgacc gcctgtacat cgtaccgcaa gttatcgaag
     3061 aataatccga atacgcttca gacggcatca gcaataccgc ccgaagccct ttaaggatgg
     3121 aagatttatg acccaataca cattgaaaca ggcaagcgtc ctgttgcagt ccaaacagat
     3181 ttccgccgtc gaactggcaa gcgcatacct tgccgccatc gccgaaaaaa atcccgccct
     3241 caacggctat atcaccatcg accaagataa aacccttgca gaagcccgtg ccgccgacga
     3301 acgtatcgcg cagggcaacg cctccgcgct taccggcgta cccgtcgcct acaaggatat
     3361 tttctgccaa accggctggc gcagcgcgtg cgcttccaaa atgctcgaca acttcatctc
     3421 cccctacacc gccaccgtcg tccaaaacct gctcgacgaa ggtatggtaa cgctcggccg
     3481 caccaatatg gatgagttcg ctatgggttc gaccaatgaa aactcattct acggtgcagc
     3541 caaaaaccca tggaatcttg agcacgtccc cggcggttcg tcaggcggtt ccgccgccgt
     3601 cgttgccgcg cgcctcgccc ctgccgcgct cggttcggac accggcggct ctatccgcca
     3661 acccgcatcg cactgcggca ttaccggcat caaacccaca tacggcacgg tttcccgctt
     3721 cggtatggtc gcctacgcct ccagcttcga tcaaaccggc ccgatggcgc aaactgccga
     3781 agactgcgcg attctgttaa acgcgatggc aggtttcgac cccaaagact ccaccagcct
     3841 cgagcgcgaa aaagaagact acacccgcga tttgaaccaa ccgctcaaag gtttgaaaat
     3901 cggcctgccc aaagaatatt tcggcgaagg caacagcgcc gatgttctga cggcattgca
     3961 aaacaccatt gatttgctga aagcccaagg cgcggaattg attgaagttt ccctgccgca
     4021 aaccaagctg tccatccccg cctactacgt cctcgcctcc gcagaagcca gcaccaacct
     4081 ttcacgttac gacggcgtac gttacggaca ccgtgccgcc caattcgccg atttggaaga
     4141 aatgtacggc aaaacccgcg ccgaaggttt cggcagcgaa gtcaaacgcc gcatcatgat
     4201 cggcacttat gtactgtcgc acggctacta cgatgcctac tatctcaaag cccaaaaact
     4261 gcgccgcctc gttgccgatg attttcagac ggcatttgca cggtgcgacc tcatcctcgc
     4321 gccgaccgca cccactgcag ccccaaaaat cggagcggat gcttcgccgg ttgaaaccta
     4381 cttgagcgat atctacacca tcgccgtcaa cctcgccgga ctgcccgcat tgaccctgcc
     4441 cgcaggcttc agcggcggcg gactgcccgt cggcgttcag cttgtcggca actacttcgc
     4501 cgaagccaaa atcctcggtg cggcgcatca aatccaactc aacagcgatt ggcacggcaa
     4561 acgacccgaa tgaagcagaa ccgcaccttt accttccccg attttcgcac cgtttacagc
     4621 tatgcgcctt tatatcggct gcaacattta aaatacacat tgcgaaaatt tttcggaaaa
     4681 aaagaaattt acgccttcga gcagtttgtc aacgcctccc ctatccgtca ggggctgttc
     4741 ctccactgcc cgcaaaatgc ctatccgctg ctgcgcgaat ttgttgacag gcgttttaac
     4801 tgcaaacgcc gtttagatgc gatgacggca gattttctca tggcggaaaa actgttcggc
     4861 acagacatcc tgcaccaaat ggaagactac cgcttccatt tggtcttggc gcacctttca
     4921 gacggcatca gcttgtggct caaccgcaac gacaactgcg tcgaagaagg cgcgtggtct
     4981 ttatctttgc gcgacgaagc aggcaaccgg ctgtatatgg cgactttcgc ctttgtcggc
     5041 acacacctgc tgacagcctc cgtacaaggg cctgcgggtg aagaagccaa agacactgtc
     5101 cgccgcataa ccaaacaact tcacggcttg cgtccccaac aactgatggt aaccgccctg
     5161 caatatttcg ccgccgtact cggcttggac ggcgcaatgg gcattgcaca aaaacatcag
     5221 gtcaaactgc gctggaaact taaaaagcgt gtcaaaatga attacgacgc attctggcag
     5281 gaatacggcg caagtttgga acgggacggc tactggcatc tcccccaaac ccccgcccgc
     5341 aaagacctta ccgacatcga aagcaaaaaa cgctcgatgt accgcaagcg ttatgaaatg
     5401 ctggacaaca tggttgcaga gatgaaggac ggtctgaaaa cagaagcacg cggcatttca
     5461 gacggcatcc aaacggaaaa accgctccgc cggacagcct gacgcgaaga ctatcgaatt
     5521 gatattttag agaaagaagc tcttatgacc tgggaaaccg taatcggctt ggaaatccac
     5581 gtccaattga acaccaaatc caaaatcttc agcggcgcat cgaccgcatt cggcgcagaa
     5641 cccaacgcgc acgccggcgt agtggaatgt gctttgccgg gcgtactgcc ggtaatgaac
     5701 cgagaagtcg ttgaaaaagc catcaaatta ggcttggctt tggatgcgaa aatcaaccgg
     5761 aaaaacgtgt tcgaccgtaa aaactacttc tatcccgact taccaaaagg ctatcaaatc
     5821 agccagttgg acttaccgat tgtcgaacac ggcaaattgg aaatcgtagt cggcgacgat
     5881 gtgaaaacca tcaacgtaac ccgtgcgcac atggaagaag acgcaggcaa gtccgtgcat
     5941 gaaggcttga acggcgcgac cggtatcgac ctgaaccgcg ccggcacgcc gctgttggaa
     6001 gtcgtatccg aacccgaaat gcgctccgcc gccgaagccg ttgcctacgc caaagccttg
     6061 cacagcttgg taacctggct gaacatttgc gacggcaata tggcggaagg ttcgttccgc
     6121 gtcgatgcca acgtatccgt tcgcccgaaa ggtcaagcgg aattcggcac gcgccgcgag
     6181 attaaaaacc tcaattcctt ccgtttcttg gagcaggcga ttaattacga agcagaagcg
     6241 caaatcgaga ttttggaaga cggcggcaaa gtacagcagg caacgatgct gtttgatccc
     6301 gaaaaaggcg aaacccgcgt aatgcgcctg aaagaagacg cgcaagacta ccgctacttc
     6361 cccgaccccg atttgctgcc cgtcatcatt tcagacgctc aaatgcaaaa agccaaagca
     6421 gaaatgcccg agctgccgaa agaaatggca gtgcgtttcg tggcggacta cggcgtgtcc
     6481 gaatacgacg cgcgcctgct caccgcaagc cgcgcacagg ccgcctattt tgaagaagcc
     6541 gccaaagaga gcggacaagg caagccgact gccaactgga tgaacggcga actcgccgcc
     6601 gcactgaaca aagaaggcat ggaacttgcc gacagcccga ttaccgctcc gcgcctcgcc
     6661 gcgctggtag gcaaaatcgc cgacggcaca ttaagcagca aattagcgaa aaaagccttt
     6721 gaagccatgt gggcagaacc cgaagccact attgccgaaa tcatcgaaaa acacggtttg
     6781 caacagatga ccgataccgg cgcggttgaa gcgatggtgg acgaagtgct agcaaacaac
     6841 gccaaagccg tggaacagtt taaatccggc aacgaaaaag ccctgaatgc gattgtggga
     6901 caagtgatga aagccagcaa aggcaaagcc aaccccgcgc aggttcaaga gctgattaaa
     6961 gccaaacttg cctgacaagc aagatgccgt ctgaaaccca gatccgacgt tcagacggcg
     7021 ttttcacagt ctgaccacat taaaacagca taaaaaacaa tattccatga accacaccgt
     7081 taccctgccc gaccaaacca cctttgccgc cggcgacggt gaaaccgttt tggccgctgc
     7141 cgcccgtcaa aacctcaatc tgccccattc ctgcaaaagc ggtgtctgcg ggcaatgcaa
     7201 agccgaactg gtcagcggcg atattcaaat aggcggacac tcggaacagg ctttatccga
     7261 agcagaaaaa gcgcaaggca agattttgat gtgctgcacc actgcgcaaa gcgacatcag
     7321 cctcaacatc cccggctaca aagccgatgc cctacccgtc cgcaccctgc ccgcacgcat
     7381 cgaaagtatg gttttcaaac acgatgtcgc cctcttgaaa cttgccctgc ccaaagcccc
     7441 gccgtttgcc ttctatgctg ggcaatacat cgatttactg ctgccgggca acgtcagccg
     7501 cagctactcc atcgccaatt cgcccgacca agaaggcatt ttggaactac acatccgcag
     7561 gcgcgaaaac ggtgtctgct cggaaatgat tttcggcagc gaacccaaag tcaaagaaaa
     7621 aggcatcgtc cgcgttaaag gcccgctcgg ttcgtttacc ctgcaggaag acagcggcaa
     7681 acccgtcatc ctgctggcaa ccggcacagg ctacgccccc atccgcagca tcctgctcga
     7741 ccttatccgc caagacagcg gccgcgccgt ccatttctac tggggcgcgc gtcatcaaga
     7801 tgatttgtat gccctcgaag aagcacaagg gttggcatgc cgtctgaaaa acgcctgctt
     7861 cacccccgta ttgtcccgcc ccggagaggg ctggcaggga agaaaagggc acgtacaaga
     7921 catcgcggca caagaccacc ccgacctgtc ggaatacaaa gtgtttgcct gcggttcccc
     7981 cactatgacc gaacaagcaa agaatctgtt tgtgcaacag cataagctac cggaaaactt
     8041 atttttctcc gacgcattca cgccgtccgc atcataattc cccggtataa agaagattcg
     8101 ggctttccgt tcggaacaca aaaaacttcc cgtccgtgtt ttcccgtgaa aaaatgccgt
     8161 ctgaaaccca attccggctt tcagacggca tatgtttttt cctgttcaag gcgacagccg
     8221 ctcgcgtatc cagccgccat ccagcaaacg gtattggatg cggtcgtgca gcctactcgg
     8281 cctgccctgc cagaactcaa gcaaatcggg aatcacaata tagccgcccc aatgcgacgg
     8341 acgcggcacg tgcaaaggat gtttgagtcc gaccgccgcc gcctttgcca ccaataccgc
     8401 cttgttcgga atcacctcgc tctgcgcact tgcccacgca cccaaacggc tctgatacgg
     8461 gcgactctca aaatattcgt ccgacaactt ctccgccagc ctttcaacac gcccttccac
     8521 gcgcacctga cgctccagct ccggccaaaa aaacgtcatc gccgcaaatg gatgagcatc
     8581 cagcgaacgc cccttgcggc tgtgataatt cgtaaagaaa acaaaacctt cagaattaac
     8641 ttccttcagc agcaccatac ggctgttggg tctaccgcgt ccgtcgactg ccgccacatt
     8701 gaccgccgtc ggctcgttga cctgtgcgcg taccgcctcg tccaaccacc gctcgaactg
     8761 ttcgatcgga ttgtcggcgc aatcgccttc cgacaattcc cgtttgctgt aatcttcccg
     8821 aatattgtgc aaatccattt accgctcctc ttgttatttg aaagattcta cccccgcacg
     8881 caaaccgatt tcaaccgttg cacaaacttt gtccccaaag ccgcagcgac gatttcatcc
     8941 gcaaaaccgc cgcatcaggt acaatatcga accgtccgac cgagggcggc attttatcaa
     9001 cccgtcctgc cgcacacgcc gcagaagaac cgccttatca ggcgagttag gaaaaatgat
     9061 gtccaagcaa cccaccagca aacgccaatg gcgcgacggc gcagccccgt ctgccaaaaa
     9121 aaccgccaaa ccgttcaaaa gcaaagccca tcccaaaggc gaaacgcgca aaaccgcatc
     9181 ccaaccttac ggacaaaaag cttcagacgg catcaaacct caaaacctcc ccaaacagcg
     9241 cgccgccaaa gccaaaaaac tcgtcgtccg caatcccaac caaaaaatta tggaacacgc
     9301 gcgcgattta aaagaacgcc gcagcgacct atcgcgcatg gaacccgaac gcctgcaaaa
     9361 agtgcttgcc gcgtccggcg tcggctcgcg ccgcgaaatg gaagaatgga tcaacaacgg
     9421 ctgggtaacg gtcaacggca aaaccgctca actgggcgac aaagtcaccc ccgacgacca
     9481 cgttaccgtc aaaggcagca tcatcaaact caaatgggcc gaccgcctgc cgcgcatcat
     9541 cctatattac aaacaagaag gcgaaatcgt ttcccgcgac gacccgcaag gccgcgtcag
     9601 catattcgac cgtctgccgc aggccgccag cagccgctgg gtcgccatcg gacgcttgga
     9661 catcaacacc agcggacttc tgattctcac cacctccggc gaactcgtcc aacgtttcgc
     9721 ccaccccagc ttcgaagtcg aacgcgaata cgccgtgcgc gtcttgggcg ggctgaccgg
     9781 cgaacaaatg cgcgtcctca ccgaagaagg cgtgatgctc gaagacggct tggcaaaagt
     9841 cgaacgcatc cgcgaacaag gcggcgaagg cgcgaacaaa tggtacaacg tcgtgattaa
     9901 agaaggccgc aaccgcgaag tgcgccgcat ttttgaaagc caagggctca ccgtcagccg
     9961 cctcgtgcgc gtcggcttcg gtcccatcgg actgcccaac cgcctcaaac gcgggcagtt
    10021 ctacgaactc aaccccgccg aagtcgccaa catcatcaaa tgggcggaca tgctgctgcc
    10081 gggcgaacgc cgccgcaaaa aagcctaaac ccgccaaaac acaaaaatgc cgtctgaaac
    10141 atctgctgtt tcagacggca ttttattcgg gcgttttcag gagaaaaggt cgagtgcttt
    10201 gacaaagacc atcaccacgc cgtaggcgag cggtatgacg gccaatgccc aacgccacca
    10261 gaccgatatg ccgccgccgg aaactttttc gcccacaagg taagcgtcgg atatggcggt
    10321 ttcgtcatcg gggttgccgc tgtgtgcggc ggttttgatg tctttttcgt ggtgtttttc
    10381 gtgtacggat ttgacggcga ggttgcacaa caaaccgata atcagcaggc acgccatgat
    10441 gtacatggtt acgctgtatg cctgtgccgc cggtatgccg ctgtcgattt ggctttggcg
    10501 tatgtaattg accagtaccg ggccgatgac ggcggcggtt gaccaggcca gcaggatgcg
    10561 tccgtgaatc gcgccgacct gataggtgcc gaacaggtct ttcaggtagg cgggaatggc
    10621 ggcaaatccg ccgccgtaca tggaaataat cacgcaaaag ccgatgatga acagggcttt
    10681 gctgccgccc tcgccgatgg agggaacggc gaaatacagc agcgagccga gtacgaagaa
    10741 gatggtgtag gtgtttttgc gtccgatttt gtcggaaacg ctcgaccaca aaaagcgtcc
    10801 gcccatgtta aacaggctca ggaggctgac gaagcctgcc gccgcacctg cgccgactgc
    10861 tgcctgcctg cctatggagg tttcggaaaa gagttcctga atcatcacgg atgcctgacc
    10921 caatacgccg atgccggcgg ttacgttcag gcacaatacc cagaacaaca gccaaaactg
    10981 cggcgttttc atggcttggg acacgttgac atgattactg ctgaccagct tgttttgcgt
    11041 tttcggcgcg gtatagcctt caggtttcca gccgtcggca ggtacgcgga tggtaaacgc
    11101 gccgaacatc atcagtgcga ggtaaagcag gcccaatacg gcaaaggttt cggcaacccc
    11161 gaccgaagcg gcgtttgaaa aggcgttcat cagtgatacg gaaagcggag aggccagcat
    11221 tgcgccgccg ccgaaaccca taatcgccaa accggtcgcc atacccggct tgtcgggaaa
    11281 ccatttcatc agtgtggaaa ccggcccgat gtagcctaag cctaagccta cgccgccgat
    11341 gacgccgttg cccaaataga gcaggaagag gttgtgcgta cgcacgccga atgcggatac
    11401 gaagaagccc aggctgaaac agcaggcggc ggcaaatatg gctttgcgcg gacctacccg
    11461 ttccatccat gtgccgaaca gggcagccga cgcgcccagc atcgcgagtg cgatactaaa
    11521 aatccaacct acggtcgtca gcttccaatc tccggccgcc gattcggtta tgccgataag
    11581 tttggtcagc ggcgcgttga atacggaata ggcgtaaatc tgcccgatgg caaggtgcac
    11641 cgccaatgct gcgggcggta cgagccaacg gttgaaaccc ggcttggcaa tgcttgcctc
    11701 acggtctaaa aacttcataa catcctcttt ctgtcagttg aaaaataaaa tttcatttgc
    11761 tcaatggaaa cttattgaaa attataaaaa aatatcgggt cgggttttta tccgtcccaa
    11821 gatgcgccgt ctgaaacatt tcgggtgtac ggaaaggttt ctgttttttc cgacaaattc
    11881 ctgcggcttt tcgcttccgg attcccgctt ttgcgggaat gacgaattaa agattatctt
    11941 aagatcaagg gactggattc ccgcttt
//
LOCUS       DAXHSF010000044        11744 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.044, whole genome shotgun sequence.
ACCESSION   DAXHSF010000044 DAXHSF010000000
VERSION     DAXHSF010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11744
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.044"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(354..776)
                     /locus_tag="ACFM8S_000918"
     CDS             complement(354..776)
                     /locus_tag="ACFM8S_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFC6314010.1"
                     /translation="MQVTSKWIDGMCFVGTTEGGHSVVMEGSAAEGKAKRGPSPLEML
                     LLGVAGCSSIDVVMIAEKQRQKVTDCRATVTAKRADDAPRVFTEIHIHFKVFGHDLKE
                     SAIERAVQMSAEKYCSASIMLGKAAKITHSFEIAGADK"
     gene            complement(894..1565)
                     /locus_tag="ACFM8S_000919"
     CDS             complement(894..1565)
                     /locus_tag="ACFM8S_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HFC6314011.1"
                     /translation="MMKTFKNIFSAAILSAALPCAYAAGLPQSAVLHYSGSYGIPATM
                     TFERSGNAYKIVSTIKVPLYNIRFESGGTVVGNTLHPTYYRDIRRGKLYAEAKFADGS
                     VTYGKAGESKTEQSPKAMDLFTLAWQLAANDAKLPPGLKITNGKKLYSVGGLNKAGTG
                     KYSIGGVETEVVKYRVRRGDDTVMYFFAPSLNNIPAQIGYTDDGKTYTLKLKSVQING
                     QSAKP"
     gene            complement(1581..2207)
                     /gene="purN"
                     /locus_tag="ACFM8S_000920"
     CDS             complement(1581..2207)
                     /gene="purN"
                     /locus_tag="ACFM8S_000920"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFC6314012.1"
                     /translation="MKNIVILISGRGSNMQAIVNAAIPNVRIAAVLSNSETAAGLQWA
                     AGQGIPTDSLNHKNFESRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCAHYEGRL
                     MNIHPSVLPSFTGLHTHERALEAGCRVAGCTIHFVTAELDCGPIVSQGIVPILDGDTA
                     DDVAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA"
     gene            complement(2314..3132)
                     /locus_tag="ACFM8S_000921"
     CDS             complement(2314..3132)
                     /locus_tag="ACFM8S_000921"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC6314013.1"
                     /translation="MNTIFKISALTLSAALALSACGKKEAAPASASEPAAASSAQGDT
                     SSIGSTMQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQ
                     EVMMKFLQEQQAKAVEKHKADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGE
                     GKQPTKDDIVTVEYEGRLIDGTVFDSSKANGGPVTFPLSQVIPGWTEGVQLLKEGGEA
                     TFYIPSNLAYREQGAGDKIGPNATLVFDVKLVKIGAPENAPAKQPAQVDIKKVN"
     gene            complement(3391..3831)
                     /locus_tag="ACFM8S_000922"
     CDS             complement(3391..3831)
                     /locus_tag="ACFM8S_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFC6314014.1"
                     /translation="MPKATFYTHVAQVPIFTCRLIARAIRDGGRILVWSDSFGQLQEL
                     DKMLWQYEAESFIPHEIWEMEEAMPSETSVLLACGGNPPRIPEGMTVLNLSDGFWNTA
                     PVLPARVLEIVGNSLEDLADARERFTAYRRSGFAIEHHGMEGKA"
     gene            complement(3894..5300)
                     /locus_tag="ACFM8S_000923"
     CDS             complement(3894..5300)
                     /locus_tag="ACFM8S_000923"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFC6314015.1"
                     /translation="MEFSTKTEILQEQQAGAQLFVCTEEAQLNHPTALALLSSLEEGQ
                     NFADTKIPTGNGLQAVAVCCLKSTGRAALNKAAAEAAKWAQNQETVNVDIHAFDEAQA
                     AAVAEAFAIAFGNAAYRFDRYKKEAKPAKFETAVFHSAHEAVVKEALRVAEAQVYGQS
                     LCRDLGNAAPNECTPEFLARTAKAEAEKLGAHAKIIEKDYIKENMGSFWSVAKGSAEA
                     PYLVELSYFGAADKEAAPVVLVGKGITFDTGGISLKPGLNMDEMKFDMCGAATVISTF
                     CAAVKLQLPINLIAIVATCENMPSGAANKPGDVVKSMKGLTIEVLNTDAEGRLILCDA
                     LTYAEQFKPKAVIDVATLTGACIVALGHDVSGVMGNNQDLVDSLLAASYNVDDKAWQL
                     PLFETYKDQLKSNFADIPNIGTPGAGTITAATFLSYFTEDYPWAHLDIAGTAWKSGAE
                     KGATGRPVPLLLNYLRNL"
     gene            5470..6585
                     /gene="lptF"
                     /locus_tag="ACFM8S_000924"
     CDS             5470..6585
                     /gene="lptF"
                     /locus_tag="ACFM8S_000924"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFC6314016.1"
                     /translation="MIYQRNLIKELSFTAVGIFVVLLAVLVSTQAINLLGRAADGRVA
                     IDAVLALVGFWVIGMTPLLLVLTAFISTLTVLTRYWRDSEMSVWLSCGLALKQWIRPV
                     MQFAVPFAVLVAVMQLWVIPWAELRSREYAEILKQKQELSLVEAGGFNSLGKRNGRVY
                     FVETFDTESGIMKNLFLREQDKNGGDNIIFAKEGNFSLNDNKRTLELRHGYRYSGTPG
                     RADYNQVSFQKLNLIISTTPKLIDPVSHRRTIPTAQLIGSSNPQHQAELMWRISLTVS
                     VLLLCLLAVPLSYFNPRSGHTYNILIAIGLFLIYQNGLTLLFEAVEDGKIHFWLGLLP
                     MHIIMFVIAIVLLRVRSMPSQPFWQAVGKSLTLKGGK"
     gene            6582..7652
                     /gene="lptG"
                     /locus_tag="ACFM8S_000925"
     CDS             6582..7652
                     /gene="lptG"
                     /locus_tag="ACFM8S_000925"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFC6314017.1"
                     /translation="MNLISRYIIRQMAVMAVYALLAFLALYSFFEILYETGNLGKGSY
                     GIWEMLGYTALKMPARAYELIPLAVLIGGLVSLSQLAAGSELTVIKASGMSTKKLLLI
                     LSQFGFIFAIATVALGEWVAPTLSQKAENIKAAAINGKISTGNTGLWLKEKNSIINVR
                     EMLPDHTLLGIKIWARNDKNELAEAVEADSAVLNSDGSWQLKNIRRSTLGEDKVEVSI
                     AAEENWPISVKRNLMDVLLVKPDQMSVGELTTYIRHLQNNSQNTRIYAIAWWRKLVYP
                     AAAWVMALVAFAFTPQTTRHGNMGLKLFGGICLGLLFHLAGRLFGFTSQLYGIPPFLA
                     GALPTIAFALLAVWLIRKQEKR"
     gene            7952..10537
                     /gene="acnB"
                     /locus_tag="ACFM8S_000926"
     CDS             7952..10537
                     /gene="acnB"
                     /locus_tag="ACFM8S_000926"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFC6314018.1"
                     /translation="MLEAYRKAAAERAALGIPALPLTAQQTADLVELLKSPPAGEGEF
                     LVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPLISPEYATELLGTMLGGYNIHAL
                     IELLDDDKLAPIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFASRA
                     KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVG
                     PIKLLEELKAKGHPVAYVGDVVGTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGG
                     KIAPIFFNTQEDSGALPIEVDVSALKMGDVVDILPYEGKIVKNGETVAEFELKSQVLL
                     DEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPQAPAESKAGFTLAQKMVGRACG
                     LPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYP
                     KPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVMPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIKQGLLTV
                     AKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSN
                     VVLMKNMIANGYQDPRTLERRIKAMEKWLANPELLEADKDAEYAAVIEINMDDIKEPI
                     IACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRAASKLLEGKADTPVRLWIAPP
                     TKMDAKQLSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
                     KNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVA
                     ETVNV"
     gene            10679..11674
                     /locus_tag="ACFM8S_000927"
     CDS             10679..11674
                     /locus_tag="ACFM8S_000927"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006591 - ornithine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFC6314019.1"
                     /translation="MNLKNRHFLKLLDFTPEEITAYLDLAAELKAAKKAGREIQRMKG
                     KNIALIFEKTSTRTRCAFEVAARDQGAGVTYLEPSASQIGHKESIKDTARVLGRMYDA
                     IEYRGFGQEVVEELAKYAGVPVFNGLTNEFHPTQMLADALTMREHSGKPLNQTAFAYV
                     GDARYNMGNSLLILGAKLGMDVRIGAPQSLWPSEGIIAAAHAAAKETGAKITLTENAH
                     EAVKNVDFIHTDVWVSMGEPKEVWQERIDLLKDYRVTPELMAASGNPQVKFMHCLPAF
                     HNRETKVGEWIYETFGLNGVEVTEEIFESPASIVFDQAENRMHTIKAVMVAALGD"
BASE COUNT         2694 a         3252 c         3059 g         2739 t
ORIGIN      
        1 aaggcgaggc aacgccgtac tggtttttgt taatccacta taattcaata aattaatata
       61 tggcttaaaa taacgggatg cccgcctccc gtcctcctgc agaagcgggc gggtatcatt
      121 ttaaaacgcg gcatttaaaa tttgaccgaa aattgttgac aatccggaat caagtctgca
      181 caataccccg acaagtccaa gtattataaa ggctgaataa agaggaaaca gcaggcagat
      241 atattcggga ggtgcagtcc gaatatatct gcttttttat gcgcctccgg attgcctgcc
      301 gcacctttcc cttcagacgg catcagccgt ttccccataa tgccgcccga tgcctattta
      361 tctgccccgg caatttcaaa actgtgggta atctttgccg ctttgcccaa cataatcgaa
      421 gccgaacagt atttttcggc agacatctga acggcgcgct caatggccga ttctttcaaa
      481 tcatgcccga atactttgaa atggatgtgg atttcggtaa acacgcgcgg cgcatcgtcc
      541 gcccgtttcg ccgtaaccgt cgcacggcag tcagtcactt tctgacgctg tttttcggca
      601 atcatcacca catcgatgct cgaacagccc gccacgccca acagcagcat ttccaaaggg
      661 ctgggcccgc gcttagcctt accttctgcc gccgacccct ccataacgac gctgtgcccg
      721 ccttccgttg ttccgacaaa gcacattccg tctatccatt ttgatgtaac ctgcatggtg
      781 tcattcctga aaatagcgtt aaaaccgctt tgcatatggc gttattgtaa acaatttcaa
      841 gcggcttatg cagaaatatg gacaaaacgg caaaaaaacg cctgaaaatc gatttacggt
      901 ttggcggact ggccattgat ctgcaccgat ttgagtttca gcgtataggt tttgccgtcg
      961 tcggtatagc cgatttgtgc cggaatattg ttcagggacg gtgcgaagaa atacattacc
     1021 gtatcgtcgc cgcgccgcac ccgatatttg acgacttcgg tttccacgcc gcctatgctg
     1081 tattttcctg tacccgcctt attcaaaccg ccgacggaat aaagtttttt gccgttggtg
     1141 attttcagcc ccggggggag tttcgcgtca tttgccgcca actgccaggc aagcgtgaac
     1201 aaatccatag ccttggggct ttgctcggtt ttgctctcgc ccgctttgcc gtaggttacg
     1261 ctgccgtcgg cgaatttggc ttccgcatac agtttgcccc tgcgtatgtc tctatagtag
     1321 gtagggtgca gggtattgcc gacaaccgta ccgccggact cgaaacggat attgtatagc
     1381 ggcactttaa tcgtcgaaac gattttgtaa gcattgccgc tgcgttcaaa tgtcatcgtg
     1441 gcgggaatgc cgtagctgcc ggaatagtgc agcacggcgg attggggcag ccctgccgca
     1501 tacgcgcacg gcagggcggc ggacaaaatg gcggcggaaa atatattttt aaaagtcttc
     1561 atcatttgct cccgcccggt ttacgccgtc aggaaacggg cggcatcggc gttttccgaa
     1621 tttctgacgc ggtttccctc gataatcagg cggccggcgg caaaatcggc aacggctttc
     1681 ggatagagtt tgtgttcgac agccaaaacc cgggcggcaa catcgtctgc cgtatcgccg
     1741 tcgagtatcg gcacaatccc ttgcgataca atcgggccgc aatccagttc ggcagtaacg
     1801 aaatggatgg tgcagccggc aacgcggcag cccgcctcca aagcgcgttc gtgcgtatga
     1861 agtccggtaa acgagggcag gacggacggg tgaatgttca tcagcctgcc ttcgtaatgg
     1921 gcgcaaaact caggggtcag aatccgcata aaacctgcca aaaccaccaa gtcgggttga
     1981 tatgcgtcga ttttttccat catggcggta tcgaaggcaa gtcgtgattc aaagttttta
     2041 tgattcaggc tgtcggtcgg aataccctga ccggccgccc attgcaaacc ggcagccgtt
     2101 tcgctgttgc tcaacacggc ggcaatacgg acgttgggaa tggcggcatt gacgattgcc
     2161 tgcatattgc tgccgcgtcc ggaaatcagg atgacgatgt ttttcataat ggtgcgcttt
     2221 tgaaagggat gccatctgaa ccgctgtttg gtggtttcag acggcatttg ccgtaaaaat
     2281 gcccgaaaac ctgtttcggg catggattcg gacttaattt acttttttga tgtcgacttg
     2341 agccggctgc ttggcgggcg cgttttcggg tgcgccgatt ttgaccagtt tcacatcaaa
     2401 taccaaagtg gcgttcggac cgattttgtc gcccgcaccc tgttcgcggt aggcaaggtt
     2461 ggacgggatg tagaacgtgg cttcgccgcc ttctttcaga agctgtacgc cttcggtcca
     2521 acccggaatc acttggctca aagggaaggt gaccgggccg ccgttggctt tgctgctgtc
     2581 gaataccgta ccgtcaatca ggcggccttc gtattccacg gtaacgatgt cgtctttggt
     2641 cggctgtttg ccttcaccct gtttggtgat tttgtattgc aggccggaag cagtggtctt
     2701 cacgccgtct ttggcggcat tttctttcag aaaggcttcg cctttttctt tattggcctt
     2761 cgcgtccgcc ttgtgttttt ctacggcttt agcctgttgt tcctgaagga atttcatcat
     2821 gacttcctga gcctgctctt cggtcatttt gatttctttg ccgtcataca ctgcctgcat
     2881 ggcttcggta aagactttca aatcgatttc cgcgccctgt tccttcattt gcttcaggga
     2941 gcgtccgatg tccacgccca tcgcatagct tgcctgctgc atcgtgctgc cgatcgaaga
     3001 ggtgtcgccc tgcgcggaag aagcggcggc aggttcggat gcagatgcgg gggcggcttc
     3061 ttttttgccg caggcggaaa gtgccaaagc ggcggaaagg gtcagtgcgc tgattttgaa
     3121 aatggtgttc atgatggatc ttcgctgtcg ataaggtcgg aaaaacggga ttatagccga
     3181 gtttgaatgt ttcaacacac aggatgacac ataaagcgtc aatcgtgtgt tgccctgttt
     3241 tggaaaggat tgaaccttcc aaaataagtt ttgattctac cgccccgagg gacagatgtc
     3301 caagtggcgg ggttcaaccg ataaggaaat tttaatcaaa tagaatcaag cctgtttaaa
     3361 ttttgtaaat gcggcatttc agacggcatt ttatgccttg ccctccatgc cgtgatgttc
     3421 gatggcaaaa ccgcttcggc ggtaggcggt aaagcgttcg cgcgcgtcgg cgagatcttc
     3481 caagctgttg ccgacgattt ccaaaacgcg cgcgggcaag accggagcgg tgttccaaaa
     3541 accgtcggac aggttcaaaa cagtcatgcc ttcgggaatt cggggcggat tgccgccgca
     3601 agcaagcagg acggatgttt cagacggcat ggcttcttcc atttcccaaa tttcgtgcgg
     3661 aataaaactc tcggcctcgt attgccaaag cattttgtcc aattcctgaa gctgcccgaa
     3721 cgaatcggac cacaccagta tcctgccgcc gtcccgaatc gcacgggcaa tcaggcggca
     3781 ggtgaaaatc ggaacttggg caacgtgcgt gtaaaaggtg gctttcggca tattgtttga
     3841 gcatttggca ggacgatgcc gtctgaaagg cttcagacgg cattgtggca aaattaaaga
     3901 ttccgcagat agttcagcag caagggaacg ggacggccgg tcgcaccttt ttccgcaccg
     3961 gatttccacg ccgtacccgc gatgtcaagg tgtgcccatg gatagtcttc ggtaaagtag
     4021 gataggaatg ttgcggcggt aatcgtgccc gcgccgggcg tgccgatgtt tggaatgtcg
     4081 gcaaagttgg atttgagttg gtctttgtag gtctcaaaga gcggcagttg ccacgctttg
     4141 tcgtccacgt tgtaggaagc ggcaagcagg ctgtcgacca aatcttgatt gttgcccatc
     4201 acgccgctga catcgtgccc caaggcaaca atacacgcac cggtcagggt ggcgacgtcg
     4261 atgacggctt tgggtttgaa ctgttcggcg taagtgagcg cgtcgcacaa aatcagacgg
     4321 ccttcagcat cggtgttcaa cacttcgatg gtcaggcctt tcatgctttt tacgacgtcg
     4381 cccggtttgt tggccgcgcc ggaaggcata ttttcacaag tggcgacgat ggcaatcagg
     4441 ttgatcggca gttgcagttt gacggcggcg cagaaggtgc tgatgacggt tgccgcgccg
     4501 cacatatcaa acttcatttc gtccatgttc agaccgggtt tgagggaaat gccgccggtg
     4561 tcgaaggtaa tgcctttgcc gaccaagacg acgggggcgg cttctttgtc ggctgcaccg
     4621 aaatagctca gttcgaccaa atagggggct tccgcgctgc ctttggcgac cgaccaaaac
     4681 gaacccatgt tttctttgat gtagtctttt tcgatgattt tggcgtgcgc gcccagtttt
     4741 tcggcttcgg ctttggcggt gcgcgctaaa aattcgggcg tgcattcgtt gggcgcggcg
     4801 ttgcccaagt cgcggcagag gctttgtccg taaacttgcg cttcggcgac gcgcaaggct
     4861 tctttaacga cggcttcgtg tgcgctgtgg aacacggcgg tttcaaattt ggcgggcttg
     4921 gcttcttttt tgtagcggtc gaaacggtag gcggcgttgc cgaacgcaat cgcaaacgct
     4981 tcggctacgg cggcggcttg cgcttcatcg aaggcatgga tgtccacatt gaccgtttcc
     5041 tgattttgcg cccatttggc ggcttcggcg gcggccttgt tcaatgcggc gcggccggtg
     5101 cttttcagac agcatacggc aacagcctgc aaaccgttgc ctgtcgggat ttttgtgtcg
     5161 gcaaaatttt gaccttcttc aagcgaagac aaaagggcaa gggcggtcgg atggtttaat
     5221 tgcgcctctt cggtgcagac aaataactgt gcgcctgcct gctgttcctg caagatttcg
     5281 gtttttgtgc taaattccac gtttattctc ctgattgaga cggttgtcgg tagttttcgg
     5341 acggcctttc gctcaaaaga ccgtctgaag acggctggca cgattgtacc ccatttgaag
     5401 caccgtctga aaccttgcgc ggacaatccg cctgcgccga accgcttacc gccccctgac
     5461 cgcgattcta tgatttatca aagaaacctc atcaaagaac tctcttttac cgccgtcggc
     5521 attttcgtcg tcctcttggc ggtattggtc tccacgcagg caatcaacct gctcggccgt
     5581 gccgccgacg ggcgtgtcgc catcgatgcc gtgttggcat tggtcggctt ctgggtcatc
     5641 ggtatgacgc cgcttttgct ggtgttgacc gcatttatca gtacattgac cgtgttgacc
     5701 cgctactggc gcgacagcga aatgtcggtc tggctatcct gcggattggc attgaaacaa
     5761 tggatacgcc cggtgatgca gtttgccgtg ccgtttgccg ttttggttgc cgtcatgcag
     5821 ctttgggtga taccgtgggc agagctacgc agccgcgaat acgctgaaat cctgaagcag
     5881 aagcaggaat tgtctttggt ggaggcaggc gggttcaaca gtttgggcaa gcgcaacggc
     5941 agggtttatt ttgtcgaaac cttcgatacc gaatccggca tcatgaaaaa cctgttcctg
     6001 cgcgaacagg acaaaaacgg cggcgacaac atcatcttcg ccaaagaagg taacttctcg
     6061 ctgaacgaca acaaacgcac gctcgaattg cgccacggct accgttacag cggtacgcct
     6121 ggacgcgccg actacaatca ggtttccttc caaaaactca acctgattat cagcaccacg
     6181 ccaaaactca tcgaccccgt ttcccaccgc cgtaccattc cgaccgccca actgattggc
     6241 agcagcaacc cgcaacatca ggcggaattg atgtggcgca tctcgctgac cgtcagcgtc
     6301 ctcctactct gcctgcttgc cgtgccgctt tcctatttca acccgcgcag cggacatacc
     6361 tacaatatct tgattgccat cggtttgttt ttaatttacc aaaacgggct gaccctgctt
     6421 tttgaagccg tggaagacgg caaaatccat ttttggctcg gactgctgcc tatgcacatc
     6481 atcatgttcg tcatcgcaat cgtacttctg cgcgtccgca gcatgcccag ccagcccttc
     6541 tggcaggcgg ttggcaaaag tctgacattg aaaggcggaa aatgaacctg atttcacgtt
     6601 acatcatccg tcaaatggcg gttatggcgg tttacgcgct ccttgccttc ctcgctttgt
     6661 acagcttttt tgaaatcctg tacgaaaccg gcaacctcgg caaaggcagt tacggcatat
     6721 gggaaatgct gggctacacc gccctcaaaa tgcccgcccg cgcctacgaa ctgattcccc
     6781 tcgccgtcct tatcggcgga ctggtctccc tcagccagct tgccgccggc agcgaactga
     6841 ccgtcatcaa agccagcggc atgagcacca aaaagctgct gttgattctg tcgcagttcg
     6901 gttttatttt tgctattgcc accgtcgcgc tcggcgaatg ggttgcgccc acactgagcc
     6961 aaaaagccga aaacatcaaa gccgccgcca tcaacggcaa aatcagcacc ggcaataccg
     7021 gcctttggct gaaagaaaaa aacagcatta tcaatgtgcg cgaaatgttg cccgaccata
     7081 cgcttttggg catcaaaatt tgggcgcgca acgataaaaa cgaattggca gaggcagtgg
     7141 aagccgattc cgccgttttg aacagcgacg gcagttggca gttgaaaaac atccgccgca
     7201 gcacgcttgg cgaagacaaa gtcgaggtct ctattgcggc tgaagaaaac tggccgattt
     7261 ccgtcaaacg caacctgatg gacgtattgc tcgtcaaacc cgaccaaatg tccgtcggcg
     7321 aactgaccac ctacatccgc cacctccaaa acaacagcca aaacacccga atctacgcca
     7381 tcgcatggtg gcgcaaattg gtttaccccg ccgcagcctg ggtgatggcg ctcgtcgcct
     7441 ttgcctttac cccgcaaacc acccgccacg gcaatatggg cttaaaactc ttcggcggca
     7501 tctgtctcgg attgctgttc caccttgccg gacggctctt cgggtttacc agccaactct
     7561 acggcatccc gcccttcctc gccggcgcac tacctaccat agccttcgcc ttgctcgccg
     7621 tttggctgat acgcaaacag gaaaaacgtt gaaccaatgc cgtctgcccc ccttcggacg
     7681 gcattggttt gtattatcat attcccgtag acagatagcc gttccctatt atagtggatt
     7741 aacaaaaacc agtacggcgt tgcctcgcct tgccgtacta ttgtactgtc tgcggcttcg
     7801 tcgccttgtc ctgatttaaa atacattacc tgtcataaca gttccatttt tgttaaaact
     7861 agtctatgat agcggtacaa atattgttta caatatttaa cgcaaatcat ttgcaacccg
     7921 acaaaagaaa aacagaaaaa ggaacaaaga gatgttagaa gcctatcgta aagccgccgc
     7981 cgagcgcgcc gcccttggta ttcccgccct tcctctgact gctcagcaaa ccgccgattt
     8041 ggttgagctg ctgaaaagcc cgcccgcagg cgaaggcgag ttcttggtcg aactgcttgc
     8101 ccaccgtgtt ccgcccggtg tggacgatgc cgccaaagtc aaagcctcat tcctggctgc
     8161 cgttgccgaa ggcagcgcgt ccagcccgct gatctccccc gaatatgcga ccgaactctt
     8221 aggtacgatg ctcggcggtt acaatatcca cgccttaatc gaactcttgg acgacgacaa
     8281 actcgcgccc attgccgcca aaggcttgaa acatacgctt ctgatgttcg attccttcca
     8341 cgacgttcag gaaaaagccg aaaaaggcaa caaatacgcg caagaagttt tgcaatcttg
     8401 ggcagatgcc gaatggttcg cctcacgcgc caaagttccc gaaaaaatca ccgttaccgt
     8461 tttcaaagtt gacggcgaaa ccaatacaga cgacctctcc cccgcgcccg acgcgtggag
     8521 tcgtcccgat attccgctgc acgcgctggc catgctgaaa aacccgcgcg acggcatcac
     8581 gcccgacaaa ccgggcgaag tcggtccgat taaattgttg gaagaactca aagccaaagg
     8641 ccatccggtt gcttacgtcg gcgacgtggt cggtactggt tcttcacgca aatccgcgac
     8701 caactccgtc atttggcata ccggcgaaga cattccgttc gtgccgaaca aacgcttcgg
     8761 cggcgtatgt ttgggcggca aaatcgcgcc gattttcttc aatacccaag aagattccgg
     8821 cgcgctgccg attgaagtcg atgtatctgc tctaaaaatg ggcgatgtcg tcgatatcct
     8881 gccttatgaa ggcaaaatcg tgaaaaacgg cgagactgtt gccgagtttg aattgaaatc
     8941 acaagtattg ctggacgaag tgcaagccgg cggccgtatc aacctgatta tcggtcgagg
     9001 tctgaccgcc aaagcgcgcg aagccctgaa actgcctgcc tctactgcat tccgcctgcc
     9061 gcaagcgcct gccgaaagca aagccggttt caccttggcg caaaaaatgg tcggccgcgc
     9121 ctgcggtctg cccgaaggac aaggcgtgcg cccgggtact tactgcgaac cgcgtatgac
     9181 gacggtcggc tcgcaagaca cgaccggccc gatgacccgc gacgagttga aagacttggc
     9241 ttgtttgggc ttctccgccg atatggtgat gcagtctttc tgccacaccg ccgcctatcc
     9301 gaaacctgtc gatgtaaaaa cccataaaga actgcccgcc tttatttcca cccgtggcgg
     9361 cgtgtcactg cgtccgggcg acggcgtcat ccactcgtgg ctcaaccgcc tgctgctgcc
     9421 cgataccgtc ggcaccggcg gcgacagcca tacccgtttc cccatcggta tttccttccc
     9481 cgccggctcc ggcttggttg cctttgccgc cgcaacgggc gtaatgccgc tcgatatgcc
     9541 cgagtctgta ttggtacgct tcagcggcaa gctgcaaccg ggcgtaaccc tgcgcgattt
     9601 ggtgaacgcc atcccgctgt acgcaattaa acaaggtttg ctgaccgttg ccaaagccgg
     9661 taagaaaaac atcttctctg gccgcatcct cgaaatcgaa ggcctgcccg atttgaaagt
     9721 ggaacaagcc tttgaattga ccgacgcatc cgccgaacgc tccgccgccg gctgtaccgt
     9781 gaagctcaat aaagagccga ttatcgagta catgaaatcc aacgtcgtgt tgatgaaaaa
     9841 catgattgcc aacggctatc aagacccgcg cactttggaa cgccgcatca aagctatgga
     9901 aaaatggctg gcaaatcccg agttgctcga agcggataaa gatgccgaat acgccgccgt
     9961 gattgaaatc aacatggacg acatcaaaga gccgattatc gcctgcccga acgacccgga
    10021 cgacgtgtgc ttcatgtccg aacgctccgg caccaaaatc gacgaagtat tcatcggttc
    10081 gtgtatgacc aacatcggcc acttccgcgc cgcctccaaa cttttggaag gcaaggcaga
    10141 cacccccgtc cgcctgtgga ttgcgccgcc gaccaaaatg gacgcgaaac aattgtccga
    10201 cgaaggacac tacggcgtac tcggacgtgc cggcgcgcgt atggaaatgc cgggttgctc
    10261 cttatgtatg ggtaatcagg cgcaagtacg cgaaggtgcg accgttatgt ccacctccac
    10321 ccgcaacttc ccgaaccgtt tgggtaaaaa cacctttgtt tacctcggtt cggcggaatt
    10381 ggcagcgatt tgctccaaac tgggtaaaat cccgaccgtt gaagaatatc aagccaatat
    10441 cggcatcatc aacgaacagg gcgataaaat ctaccgctat atgaacttca acgaaatcga
    10501 cagctacaac gaagtagccg agaccgtgaa cgtttaatcc ccgtcatccg tatgaagtaa
    10561 gggattgacc gcaatgccgt ctgaacaacc ttcagacggc attgcaacat tccgctaacc
    10621 cttctttccg caaacgctgc aaatacggcg ttcacgcccc cacataaagg aaacgacagt
    10681 gaacctgaaa aaccgccatt ttctgaaact tttagacttc acgccggaag aaatcaccgc
    10741 ctacctcgac cttgccgccg aattgaaagc cgccaaaaaa gcagggcgcg agattcagcg
    10801 gatgaaaggg aaaaacatcg ccctgatttt tgaaaaaacc tctactcgga cgcgctgcgc
    10861 gtttgaagtc gccgcgcgcg atcaaggcgc gggagtgact tatttagagc cgtccgccag
    10921 ccaaatcggg cataaggaaa gcatcaaaga caccgcccgc gtgttgggca ggatgtacga
    10981 tgccatcgaa tatcgcggtt tcggtcagga agttgttgaa gaattggcga aatacgcggg
    11041 cgtacccgtg ttcaacgggc tgaccaacga gttccatccc acacaaatgc ttgccgacgc
    11101 actgactatg cgcgaacaca gcggcaaacc tttgaaccaa accgcgtttg cctacgtcgg
    11161 cgacgcgcgt tacaacatgg gcaattccct gctgatttta ggggcaaaat tggggatgga
    11221 cgtgcgtatc ggcgcaccgc aaagcctgtg gccgtctgaa ggcattattg ccgccgcaca
    11281 cgccgccgcc aaagaaaccg gcgcaaaaat taccctgacc gaaaacgcgc atgaagccgt
    11341 gaagaatgtt gattttattc ataccgatgt gtgggtcagc atgggcgagc cgaaagaagt
    11401 ctggcaggaa cgcatcgatt tgctgaaaga ttaccgcgtt acgcccgaac tgatggcggc
    11461 atcgggcaat ccgcaagtca aattcatgca ctgcctgccc gccttccaca accgcgaaac
    11521 caaagtcggc gaatggattt acgaaacctt cgggctgaac ggtgtggaag ttacagaaga
    11581 aatattcgaa agccccgcca gcatcgtgtt cgatcaggcg gaaaaccgta tgcacacgat
    11641 taaagcggta atggtcgcgg ctctgggcga ctgacagaac tgtgcctgtt taaattcatc
    11701 cgcaacacag ataccgtctg aacacgatgt tcagacggta tcca
//
LOCUS       DAXHSF010000045        11691 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.045, whole genome shotgun sequence.
ACCESSION   DAXHSF010000045 DAXHSF010000000
VERSION     DAXHSF010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11691
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.045"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            98..814
                     /locus_tag="ACFM8S_000928"
     CDS             98..814
                     /locus_tag="ACFM8S_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFC6314020.1"
                     /translation="MNFENDDIIHAPTTSSLILEERHDSELFRVYALILDGITDQVLL
                     PGKKLTESELCRQMVCSRNTVRGALSLLAHDKIVDLQPNRGAFVHVPDLKEMQDVFNA
                     RIEMETMILNILADLPDLETRLKPLYAMIRREEEASGRGDRVGWNRLSNAFHVELARL
                     VGNDVLFDIMNTLCARSSLIVAVAGVHREEKHAINTHTHSEHREILDLLLAGRRNRVV
                     KILRRHLGNCMERLEKTLED"
     gene            963..1865
                     /locus_tag="ACFM8S_000929"
     CDS             963..1865
                     /locus_tag="ACFM8S_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HFC6314021.1"
                     /translation="MDSLMTLISVLIPMFAGFFIRVPKPYLPALDKVLSVLVYAVLLL
                     IGVSLSRVEDLGSRLDDMALTVLWLFVCTVGTNLLALAVLGKLFPWRIKGKGKGVSVG
                     VSGSVGQLGCVLLGFASGKLMRDIWMPSESAGMYCLMLLVFLIGVQLKSSGLSLRQVL
                     VNRRGIRLSVWFMLSSLSGGLLFAASADGVSWTKGLAMASGFGWYSLSGLVMTEAYGA
                     VWGSIMLLNDLARELFALAFIPLLMKRFPDAAVGVGGATSMDFTLPVIQGAGGLEVVP
                     VAVSFGVVVNIAAPFLMVVFSTLG"
     gene            1908..2681
                     /locus_tag="ACFM8S_000930"
     CDS             1908..2681
                     /locus_tag="ACFM8S_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFC6314022.1"
                     /translation="MKPKIQRHGEILSLVRRHQFMSVDELAAALDVTPQTIRRDIREL
                     EEGGSLKRHHGGASSGGNLPEGLPASRQTRCQNEKNAIARLIAEHIPDGSSLFVSIGT
                     TMEAVASELVRRRGSLRIITNNIHVASIVSARTDYTVIITSGVVRPLDGGITGVATVD
                     FINQFKVDYAVMSTHGVESDGSLLDDDYKEVSVMQAMIANARVRFLGVDHSKFRSNAL
                     VRLGDITAFDKVFTDRLPDTAMQKMLKEAGVECLIADAV"
     gene            2793..4481
                     /locus_tag="ACFM8S_000931"
     CDS             2793..4481
                     /locus_tag="ACFM8S_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HFC6314023.1"
                     /translation="MLNKDQFADNHFIRTFIEEDLKSGKHETIQTRFPPEPNGYLHIG
                     HAKSICLNFGLAYIYDGLCNLRFDDTNPEKENDEYVNAIKEDVEWLGFHWAGEPRFAS
                     NYFDQLYDYAVGLVKDGKAYVDDLTPEEMREYRGTLTEAGKNSPYRDRSIEENLDLFT
                     RMKNGEFPDGSKTLRLKIDMASGNINMRDPVIYRIRRAHHHNTGDKWCIYPMYDYTHC
                     ISDAIEGITHSLCTLEFEAHRPLYDWVLDNIPAPHATRPRQYEFSRLELLYTITSKRK
                     LNQLVVEKHVSGWDDPRMPTISGMRRRGYTPEGLRLFAKRAGISKSENIVDMSVLEGA
                     IREELENSAPRLMAVLNPLKVTLTNFETGRTQSRRAAFHPNHEEMGDREVPVSQTIYI
                     EADDFAENPPKGFKRLIPGGEVRLRHGYVIKCDEVVKDEAGNVVELKCSIDHDTLGKN
                     PEGRKVKGVIHWVSAEHAAEIKVRLYDRLFTVERPDAVRGEDGEYLPFTDFLNPESVK
                     EITAYAEPAAKDLPAESRWQFERIGYFVTDRKDHGKDTPVFNRTVTLKDSWQPK"
     gene            4574..5530
                     /gene="gshB"
                     /locus_tag="ACFM8S_000932"
     CDS             4574..5530
                     /gene="gshB"
                     /locus_tag="ACFM8S_000932"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFC6314024.1"
                     /translation="MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSGELSV
                     KNGLVVADASAFEFLGAQDDHDKNWFAAADKVQTALKAFDAVIMRTDPPFDMQYLYAT
                     QLLTLAEQQGAKVFNSGQSMRDFNEKLAILNFSRFTAPTLVTTRSADVRAFLKEHGDI
                     IIKPLDGMGGMGIFRLTEKDPNIGSILETLMRLDSRTIMAQRYIPEIVHGDKRILIIG
                     GEVVPYALARIPQNGETRGNLAAGGRGVAQELDRRDREIAETLAPELKRRGILLAGLD
                     VIGSNLTEVNVTSPTGFQEIMKQKGFDVAAMFADAVAAWSVR"
     gene            5842..6225
                     /locus_tag="ACFM8S_000933"
     CDS             5842..6225
                     /locus_tag="ACFM8S_000933"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFC6314025.1"
                     /translation="MEPSSYAAEKKGKGGIRRVINAFGYSIDGIAAAYRYEAAFRQVL
                     WLNVLLVCAAFFWVSETAVRLPLIIASFVSVIVELFNTAVEAAVDHTSTEKHELAKRA
                     KDAGSAAQLFAMLMLAAVWLSALFG"
     gene            6340..6813
                     /locus_tag="ACFM8S_000934"
     CDS             6340..6813
                     /locus_tag="ACFM8S_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HFC6314026.1"
                     /translation="MKKLLAAVMMAGLAGAVSAAGVHVEDGWARTTVEGMKIGGAFMK
                     IHNDEAKQDFLLGGSSPVADRVEVHTHINDNGVMRMREVKGGVPLEAKSVTELKPGSY
                     HVMFMGLKKQLKEGDKIPVTLKFKNAKAQTVQLEVKIAPMSAMDHGHHHGEAHQH"
     gene            6939..8042
                     /gene="mnmA"
                     /locus_tag="ACFM8S_000935"
     CDS             6939..8042
                     /gene="mnmA"
                     /locus_tag="ACFM8S_000935"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFC6314027.1"
                     /translation="MNTTANPSNIIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWED
                     DDNDEYCSIKQDSFDAIAVADIVGIDIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDV
                     LCNAEIKFKCFLDYAVGQGADTIATGHYARKEVRNGVHYLLKGLDRNKDQSYFLYRLK
                     PFQLERAIFPLGGLEKPEVRRLAAEFNLPTAAKKDSTGICFIGERPFREFLQKYLPTD
                     NGKMVTPEGKTVGEHVGLMFYTLGQRKGLGIGGAGEPWFVAAKDLTKNELIVVQGHDH
                     PLLYTRSLVMNDLSFTLPERPKAGRYTCKTRYRMADAPCELRYLDEETAELVFDEPQW
                     AVTPGQSAVLYDGDICLGGGIIQTTDKPVIITR"
     gene            8114..9784
                     /locus_tag="ACFM8S_000936"
     CDS             8114..9784
                     /locus_tag="ACFM8S_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFC6314028.1"
                     /translation="MEKIWLDSYEQGVNAEIDITQYGSVSDVFCQSVKKFAGQPAFQN
                     MGKTLTYAETGKLATDFASYLQNVLKLPRGERVAIMMPNVLQYPIALFGILQAGLVAV
                     NTNPLYTPRELEHQLKDSGAAAIIVLENFANTLELVLPRTQIKHVIVASVGEMFGLLK
                     GSLINFIIRKIKKMVPEYRIRETVSFQTALKEGAKHVFQPVALNREDTALLQYTGGTT
                     GVAKGAVLSHGNICANMLQAKEWIKNQLREGKETVIAALPLYHIFALTVNLMIFANAG
                     SKIVLITNPRDMKGFIGELKKQRVNVFIGVNTLFNAMVNRPDFAEVDFSELRLTLGGG
                     MATQKAVAEKWKKITGTPIVEAYGLTEASPGVCCNPLNIESYSGSIGLPVSSTEVELR
                     DANGKEVPVGQPGELWVKGPQVMQGYWNRPEETAKAIDARGFLETGDIAVMDEKGWLK
                     LVDRKKDLVVVSGFNVYPNEIEEVIAHHGKVMEVACIGVPDEKTGEALKVFVVKKDPS
                     LTKEELIAFCRTELTAYKVPKNIEFRDELPKSNVGKILRRELRQSTGK"
     gene            9896..11530
                     /locus_tag="ACFM8S_000937"
     CDS             9896..11530
                     /locus_tag="ACFM8S_000937"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFC6314029.1"
                     /translation="MTKFIFVTGGVVSSLGKGIAAASIAAILESRGLNVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDSTMTRRNSFSTGQVYENVIAKE
                     RRGDYLGGTVQVIPHITDEIKRRIHEGAAGYDVAIVEIGGTVGDIESLPFLEAIRQMR
                     SQLGRSNTLFAHLSYVPYIAAAGEIKTKPTQHTVKEMLSIGLQPDILICRMDREMPAD
                     ERRKIALFCNVEERAIVGSYDVDSIYECPEMLHNQGIDSIITEQLQLNVQQADLTAWK
                     KIVHAIQNPKHTVKIAMVGKYVDLTESYKSLIEALKHAGIHTETDVQITFVDSESIEK
                     NNGDVSMLKDMDAILVPGGFGSRGVEGKIAAVRYARENNVPYLGICLGMQIALIEYAR
                     DVAGLKGANSTEFDLKCAAPVVALIDEWQTADGSVETRDESADLGGTMRLGAQEVELK
                     AGSLAAKIYGSEHIRERHRHRYEVNNNYVPTLEQAGLVVGGVSAGRERLVETIELPNH
                     PWFFACQFHPEFTSNPRKGHPLFTAFVKAALNNKKA"
BASE COUNT         2884 a         3154 c         3102 g         2551 t
ORIGIN      
        1 aatttgtgca atgctttaat tcctctgaca cgatttgaaa cacagccgcc ttaaaatatc
       61 ttaaaaaatt tgaaagcgta aaaatacgga aaacatcatg aactttgaaa acgacgacat
      121 tatccatgcg ccgactacgt cttccctgat tctcgaagag cggcacgatt ctgagctgtt
      181 ccgtgtttac gccctgattt tggacggcat taccgatcag gtgctgctgc ccggcaaaaa
      241 gctgaccgaa tccgaacttt gccgtcagat ggtgtgttcg cgcaacaccg tccgcggcgc
      301 gctgtcgctt ttggcgcacg acaagattgt cgatttgcaa cccaacaggg gcgcgttcgt
      361 ccacgttccc gatttgaaag aaatgcagga tgtgttcaat gcgcgcatcg aaatggagac
      421 gatgattttg aatatcctcg cagatttgcc ggatttggaa acgcgcctca agccgcttta
      481 tgcgatgata cggcgcgaag aagaggcctc cggcaggggc gaccgcgttg gctggaaccg
      541 cctgtccaat gccttccacg tcgaactggc gcgcctggtg ggcaacgatg tgctgttcga
      601 cattatgaac acgctgtgcg cgcggtcttc cctgattgtc gccgtggcgg gcgtgcatcg
      661 cgaggaaaaa cacgccatca atacgcatac gcattcagaa caccgcgaaa tcctcgacct
      721 gctgctggcg ggcaggcgca accgcgtggt caaaatcctg cgccgccatt tgggcaactg
      781 tatggagcgt ttggaaaaga ctttggaaga ttgaatgccg gagcggataa gccgcctgaa
      841 ccttcaggcg gttttttaat ggcgaacctg atgccgtctg aaatatggat gcgggtatct
      901 gcaattttca gacggcaatt tttaaaccgc acatatcatg cggcaataaa ggaggtcagg
      961 agatggacag cctgatgaca ttgatttcgg ttttgatacc gatgtttgcc ggatttttta
     1021 tccgtgtgcc caagccttac ctgcccgctt tggataaggt gctatcggtt ttggtgtatg
     1081 ccgtgctgct gctgatcggc gtatcgttgt cgcgcgtgga ggatttgggt tcgcggttgg
     1141 acgatatggc gttgacggtt ctgtggctgt ttgtttgtac ggtcgggacg aacctgcttg
     1201 ctttggcagt gttgggaaag ttattcccgt ggcggataaa ggggaaaggg aagggcgttt
     1261 cggtcggtgt gtcgggcagt gtggggcagc tcggatgcgt gctgctcgga tttgcatccg
     1321 gcaaactgat gcgcgatatt tggatgccgt ctgaaagcgc gggcatgtat tgtctgatgc
     1381 tgctggtgtt cctcatcggc gtacagctca aaagcagcgg cctatcgttg cggcaggttt
     1441 tggtcaaccg caggggtatt cggttgtcgg tctggtttat gctttcatct ctttcgggcg
     1501 ggctgctgtt tgccgcatcg gcagacggtg tgtcgtggac gaaaggtttg gcgatggctt
     1561 ccggcttcgg ttggtattcc ctctcgggtt tggtcatgac cgaggcttac ggcgcggtat
     1621 ggggcagcat catgctgctg aacgatttgg cacgagagct gtttgcactg gcatttatcc
     1681 cgctgctgat gaagcgtttt ccggatgcgg cggtgggggt tggcggtgcg accagtatgg
     1741 attttacatt gcccgtgatt cagggtgcgg gcggtttgga agtcgtgccg gtagcggtca
     1801 gcttcggcgt ggtggtcaat attgccgccc cgtttctgat ggtggtgttt tccacgctgg
     1861 gctgaacgcg gtaaaatcgg catcccgatg caaggaggca gaaaacgatg aaaccgaaaa
     1921 tccaaaggca cggagagatt ttaagccttg tccgccggca tcagtttatg tcggtggacg
     1981 agcttgccgc cgcattggac gttaccccgc agacgatacg ccgcgacatc cgcgagttgg
     2041 aggagggcgg cagcctgaaa cgccatcacg gcggcgcatc ttcgggcgga aacttgccgg
     2101 aggggctgcc tgccagccgc caaacccggt gtcaaaacga aaaaaacgcc attgcccggc
     2161 tgattgcgga acacatcccc gacggttcgt cgctgtttgt cagtatcggc acgacgatgg
     2221 aagccgtggc atcggagctg gtcaggcggc gcggcagcct gcggattatt accaacaata
     2281 tccacgtcgc ctccatcgtt tcggcgcgga cggattacac ggtcattatc acttccggag
     2341 tcgtccgccc tttggacggc ggtattaccg gcgtggcaac cgtcgatttt atcaaccagt
     2401 tcaaagtcga ttatgccgtg atgagtacgc acggcgtgga aagcgacggt tcgcttttgg
     2461 acgacgacta caaagaagtc agcgtgatgc aggcgatgat tgccaatgcg cgcgtccgct
     2521 tcctcggcgt ggaccacagt aaattccgca gcaacgcgct ggtcaggctc ggcgacatta
     2581 cggcgtttga caaagtattt accgaccggc tgcccgatac cgcgatgcag aagatgctga
     2641 aagaggcggg ggtggaatgc ctgattgccg atgccgtctg aacgctatgt caaagcgcgc
     2701 aagtcgggta caataaacac atcatcaaac cgcttcagac ggcataagga accgccccaa
     2761 tgccgtctga agccatctgt tcaaagaaaa ctatgctcaa taaagaccaa tttgcggaca
     2821 accattttat ccgcaccttc atcgaagaag acctcaaaag cggcaaacac gaaaccatcc
     2881 aaacccgttt tccgccggag cccaacggct acctgcacat cggacacgcc aaatccatct
     2941 gcctgaactt cggtttggcg tatatttacg acggcttgtg caacctgcgt tttgacgata
     3001 ccaaccccga aaaagaaaac gacgaatacg tcaacgccat caaagaagac gttgagtggc
     3061 tcggtttcca ttgggcgggc gagccgcgtt tcgcttccaa ctatttcgac cagctttatg
     3121 actacgccgt cggtttggtt aaagacggca aagcgtatgt cgatgatttg acgccagaag
     3181 aaatgcgcga ataccgcggt acgctgaccg aagcaggcaa aaacagccct tatcgcgacc
     3241 gcagtatcga agaaaacctc gacctgttca cgcgcatgaa aaacggtgag ttccccgacg
     3301 gcagcaaaac cctgcgcctg aaaatcgaca tggcatccgg caacatcaat atgcgcgatc
     3361 cggtcatcta ccgcatccgc cgcgcccatc accacaacac cggcgacaaa tggtgcatct
     3421 atccgatgta cgactacacg cattgtattt ccgatgccat cgaaggcatc acgcattcct
     3481 tgtgtacgct cgaatttgaa gcccatcgtc cgctgtatga ctgggtgttg gacaacatcc
     3541 ccgcgccgca cgccacacgt ccgcgccaat acgagttttc acgcttggag cttttgtaca
     3601 ccattacctc caaacggaaa ttgaatcagt tggttgtgga aaaacacgtt tccggctggg
     3661 acgatccgcg tatgccgacc atttccggta tgcgccgacg cggctacacg cccgaagggc
     3721 tgcgcctgtt tgccaaacgc gccggtattt ccaaatctga aaacatcgtc gatatgagcg
     3781 tgttggaagg cgcgattcgc gaagagttgg aaaattccgc cccacgcctg atggcggtgt
     3841 tgaacccgct caaagtaacc ctgaccaact ttgaaaccgg cagaacccaa agccgccgtg
     3901 ccgcgttcca tccgaaccac gaagaaatgg gcgatcgcga agtacctgtt tcacaaacca
     3961 tttacatcga agccgacgac tttgccgaaa atccgcccaa aggcttcaaa cgcctgattc
     4021 ccggcggcga agtacgcttg cgccacggct atgtcatcaa gtgcgatgaa gtcgtcaaag
     4081 acgaagcagg caatgtggtt gaactcaaat gcagcatcga ccacgacacc ttgggcaaaa
     4141 atccagaagg ccgtaaagtt aaaggcgtga ttcattgggt ttccgccgaa cacgccgccg
     4201 aaatcaaagt ccgtctgtac gaccgcctct ttaccgtcga gcgtcccgat gccgtgcgcg
     4261 gcgaagacgg cgaatacctg ccgttcaccg atttcctcaa tccggaatcc gtcaaggaaa
     4321 tcaccgccta cgccgaacct gccgccaaag atttgccggc agaaagccgt tggcagttcg
     4381 agcgtatcgg ctacttcgta accgaccgta aagaccacgg caaagacacg ccggtgttta
     4441 accgcacggt cacgctgaaa gattcttggc agcctaagta aaaccccatc cttgccgtct
     4501 gaatattgtt cggacggcat ttcccctttg cccgcgaaat gcggcacatt cggcacaccg
     4561 gaaaggaaat atgatgaaag tcctctttat cgccgacccg atggcaagtt tcaaaaccta
     4621 caaagacacc acctacgcca tgatgcgcga gatggcaaaa cgcggttggc ggctgtttca
     4681 taccttgagc ggggaattgt cggttaaaaa cggtctggtg gttgctgatg cgtctgcttt
     4741 tgagtttctc ggcgcacaag acgaccacga caaaaactgg tttgccgccg ccgacaaagt
     4801 tcagacggca ttgaaagcat ttgatgccgt gattatgcgt accgatccgc cgttcgatat
     4861 gcaatacctc tacgccaccc aattactgac gctggcggaa cagcagggcg cgaaagtctt
     4921 taacagcgga cagtcgatgc gtgacttcaa tgaaaaactg gcgattttga acttcagccg
     4981 ctttaccgcg cccacgctgg taacgacccg ttccgccgat gtccgcgcat ttttgaaaga
     5041 acacggcgac attatcatca aaccgctgga cggtatgggc ggtatgggta ttttccgcct
     5101 gaccgaaaaa gaccccaaca tcggcagcat cctcgaaacc ctgatgcggc ttgattcccg
     5161 caccattatg gcgcaacgct acattcccga aatcgtacac ggtgacaaac gcatcctgat
     5221 tatcggcggc gaagtcgtcc cctatgcttt ggcgcgtatc ccgcaaaacg gcgaaacacg
     5281 cggcaacctt gcggcaggcg ggcgcggcgt ggcgcaggaa ttggacagac gcgaccgcga
     5341 aattgccgaa acgctcgccc ccgaactcaa acggcgcggc atcctgctgg ccggtttgga
     5401 cgttatcggc agcaacctga ccgaagtcaa cgtaaccagc ccgaccggat tccaagaaat
     5461 tatgaaacaa aaaggtttcg acgtggcggc aatgtttgcc gatgccgttg ccgcgtggtc
     5521 ggtacgttaa accgatgccg tttgaaaggc ttttgcttca caaccgtttg gtttgttcgg
     5581 gcaggcaggg ttttctctca gccggcaggg atgcggttaa tgccgtacgg acaccccgcc
     5641 gccccgtttt cagacggtat atatagtgga ttaacaaaaa tcaggacaag gcggcgaagc
     5701 tgcagacagt acaaatagta cggaaccgat tcactcggtg cttcagcacc ttagagaatc
     5761 gttctctttg agctaaggcg gggcaacgcc gtacaggttt ttgttaatcc actatattga
     5821 gaacattttg aaaggatacc gatggaacct tcctcctacg cggcagaaaa aaaaggcaaa
     5881 ggcggcatca ggcgcgtcat taacgcattc ggctattcga tagacggcat cgccgccgcc
     5941 taccgttacg aagcggcatt ccgtcaggtt ttgtggctga acgtactgct ggtgtgcgcg
     6001 gcattttttt gggtttccga aaccgccgtc cgcctgccgt tgattatcgc gtcttttgtg
     6061 tcggtcattg tcgaactgtt caacaccgcc gtcgaagctg ccgtcgatca tacttcgacc
     6121 gaaaaacacg agctggccaa acgcgccaaa gacgcaggtt ctgccgcaca attgttcgcg
     6181 atgctgatgt tggcggcggt ttggctgtct gccctgttcg ggtaaaacgc ttgcagcagg
     6241 atttgtaatc ctttaggatt agtatgtggt atttattatt cacttaatcc actataagat
     6301 taagcacatt ccccgtctga tattataagg agcagaaaga tgaaaaaatt attggcggcc
     6361 gtgatgatgg caggtttggc aggcgcggtt tccgccgccg gagtccacgt tgaggacggc
     6421 tgggcgcgca ccaccgtcga aggtatgaaa ataggcggcg cgttcatgaa aatccacaac
     6481 gacgaagcca aacaagactt tttgctcggc ggaagcagcc ccgttgccga ccgcgtcgaa
     6541 gtgcataccc acatcaacga caacggcgtg atgcggatgc gcgaagtcaa aggcggcgtg
     6601 cctttggagg cgaaatccgt taccgaactc aagcccggca gctatcacgt gatgtttatg
     6661 ggtttgaaaa aacaattaaa agagggcgac aagattcccg ttaccctgaa atttaaaaac
     6721 gccaaagcgc aaaccgtcca actggaagtc aaaatcgcgc cgatgtcggc aatggaccac
     6781 ggtcatcacc acggcgaagc gcatcagcac taatctgctg aaaatatttg aaatgccgtc
     6841 tgaaaaagcc cgggctttca gacggcattt ttatgaccgc ctttaaaatg tgttaaaatc
     6901 tgcctttaaa aaccgccgtt tccaaccata ttctacgcat gaatacgaca gcaaaccctt
     6961 ccaatatcat cgtcgggctc tccggcggtg tcgattcttc cgtaaccgcc gccctgctca
     7021 agcagcaggg ttatcaagtg cgcggtgtgt tcatgcagaa ctgggaagac gacgacaacg
     7081 acgaatattg cagcatcaaa caggattcgt ttgatgccat cgccgttgcc gatattgtcg
     7141 gcatcgacat cgacatcgtt aatttcgccg cgcaatataa agacaaagtc tttgcttatt
     7201 ttcttcagga atacagtgcg gggcgcacgc cgaatcccga tgtgttgtgc aacgccgaaa
     7261 tcaaattcaa atgctttttg gattacgccg tagggcaggg cgcggatacc atcgccaccg
     7321 gacactatgc gcgcaaagaa gtccgcaacg gcgtgcatta cctgctcaaa ggtttggatc
     7381 gaaacaaaga ccaaagctat ttcctctacc gcctcaagcc tttccaactc gagcgcgcga
     7441 tttttccgtt gggcggtttg gaaaaacctg aagtccgccg ccttgccgcc gaatttaatt
     7501 tgccgaccgc cgccaaaaaa gacagcacgg gcatctgctt catcggcgag cgtccgttcc
     7561 gcgagttcct gcagaaatac ctgccgaccg ataacggcaa aatggttacg cccgaaggga
     7621 aaaccgtcgg cgaacacgtc gggctgatgt tttacacatt gggtcagcgc aaaggtttgg
     7681 gcatcggcgg cgcgggcgaa ccgtggtttg ttgcggctaa agatttgacg aaaaacgaac
     7741 tgattgtcgt gcaaggacac gaccatccgc tgctctatac ccgcagcctt gtgatgaacg
     7801 atttgagttt cacgcttccc gaacgcccga aggcaggacg ctatacctgc aaaacgcgtt
     7861 accgtatggc ggacgcgcct tgtgagttgc gctatttgga tgaagaaacc gccgagctgg
     7921 tgtttgacga accgcaatgg gcggttacgc cgggccagtc tgccgtgctg tacgacggcg
     7981 acatctgttt gggcggcggc atcattcaaa cgaccgacaa gcctgtcatc atcacacgat
     8041 aaaacttatg ccgtctgaaa cagttttcag acggcattgt tccgctcaat tccactttaa
     8101 aagaccgata cctatggaaa aaatctggtt agacagttac gaacagggcg tgaatgccga
     8161 aatcgacatc acgcaatacg gttccgtcag cgatgtattt tgccaaagtg tcaagaaatt
     8221 tgccggccaa cctgcttttc aaaacatggg caaaacgctg acttatgccg aaaccggcaa
     8281 actggcgacc gatttcgcct cttatctgca aaacgtcctc aagctgccgc gcggcgaacg
     8341 tgttgccatt atgatgccga acgtattgca atatccgatt gcccttttcg gtattttgca
     8401 ggcaggtttg gtagcggtga acaccaatcc gctctatacc ccgcgcgagc tggagcatca
     8461 gttgaaagac agcggcgcgg ccgccatcat cgttttggaa aatttcgcca acacgctgga
     8521 gctggtgctg ccgcgcacgc agatcaaaca cgtcatcgtc gcctctgtcg gcgaaatgtt
     8581 cgggctgctt aaaggttcgc tgatcaattt catcatccgg aaaatcaaga aaatggtgcc
     8641 cgaataccgt attcgggaaa ccgtttcctt tcagacggca ttgaaagaag gggcgaagca
     8701 cgttttccaa cctgtcgcat taaaccgcga agataccgcg ctgttgcaat acacgggcgg
     8761 cacgacaggt gttgccaaag gggcggtgct gagtcacggc aacatctgcg ccaatatgct
     8821 tcaggcaaaa gaatggatta aaaaccagtt gcgcgagggc aaagaaaccg ttatcgccgc
     8881 cctgccgctg tatcatattt ttgccttaac cgtgaatctg atgatttttg ccaatgccgg
     8941 ctcgaaaatt gtcctgatta ccaacccgcg cgatatgaaa ggctttatcg gcgaactgaa
     9001 aaaacagcgg gttaatgtat ttatcggcgt aaacacgctg tttaacgcga tggttaaccg
     9061 gcccgatttt gccgaagtcg atttttcaga attgcggctg actttgggcg gcggtatggc
     9121 gacccaaaaa gccgttgccg aaaaatggaa aaaaatcact ggcacgccca tcgtcgaagc
     9181 ctacggtttg accgaagcca gccccggcgt gtgctgcaac cccttaaaca tcgaatcata
     9241 cagcggcagc atcggtttgc ccgtctcgtc caccgaagtc gaactgcgcg acgcaaacgg
     9301 caaagaagtc cccgtcgggc agccgggcga attgtgggta aaaggccctc aagtgatgca
     9361 aggctactgg aaccgccccg aagaaaccgc caaagccata gacgcgcgcg gcttcttgga
     9421 aaccggcgat attgccgtca tggacgaaaa aggctggttg aagctggtcg atcgtaagaa
     9481 agacctcgtc gttgtttccg gattcaatgt ttatccgaac gaaatcgagg aagtcatcgc
     9541 gcatcacggc aaagtcatgg aagtcgcctg tatcggcgtt cccgacgaaa aaaccggcga
     9601 ggcactcaaa gtattcgtcg tcaaaaaaga cccgtcattg accaaagaag aacttatcgc
     9661 cttttgccgc accgaattaa ccgcatataa agtaccgaaa aatatcgaat tccgcgacga
     9721 gttgcccaag tccaacgtcg gcaaaatcct gcgccgcgag ttgcggcaaa gtaccgggaa
     9781 ataaagaaaa gatgccgtct gaaaacagcc gtccaccgtt cagacggcat ccgtccgttt
     9841 gccagaaccg cgcgcttcac gttaaaatca cgcattccaa catgggtatt ctatcatgac
     9901 caaattcatt ttcgtaaccg gcggcgttgt ctcctcactg ggtaaaggta tcgccgccgc
     9961 ttctattgcc gccatcctcg aatcgcgcgg cttgaacgtt accatgctca agctcgatcc
    10021 ttatatcaac gtcgatcccg gcacgatgag cccgtttcaa cacggcgaag tgttcgtaac
    10081 cgacgacggc gcggaaaccg acctcgactt gggacactac gaacggttta tcgattccac
    10141 gatgacccgc cgcaacagct tcagcacggg gcaggtgtac gaaaacgtca tcgccaaaga
    10201 acgacggggc gactacctcg gcggcacggt tcaagtcatt ccgcacatta ccgacgaaat
    10261 caaacgccgc atccacgaag gcgcggcagg ttatgatgtg gcgattgtcg aaatcggcgg
    10321 cacggtcggc gacatcgaat cgctgccgtt tttggaagcc atccgccaaa tgcgaagcca
    10381 gttgggacgc agcaacaccc tgttcgccca cttgagctac gtcccctaca tcgccgccgc
    10441 cggcgaaatc aaaaccaaac cgacccagca tactgttaaa gaaatgttga gcatcggttt
    10501 gcaaccggac atcctgatct gccgtatgga cagagaaatg cctgcggacg agcgccgtaa
    10561 gattgcattg ttctgtaacg ttgaagaacg tgccatcgtg ggcagttacg acgtggacag
    10621 catttacgaa tgccctgaaa tgctgcacaa ccaaggcatc gacagcatta ttaccgagca
    10681 gttgcagctt aatgtgcagc aggcggattt gaccgcgtgg aaaaaaatcg tccacgccat
    10741 ccaaaacccg aaacacaccg tcaaaatcgc catggtcggc aaatacgtcg atttgaccga
    10801 gtcctacaaa tcgctgatcg aagccttgaa acacgcaggc attcacaccg aaaccgacgt
    10861 acaaattacc tttgttgaca gcgaaagcat cgagaaaaac aacggcgacg tttctatgct
    10921 taaagatatg gacgccatcc tcgttcccgg cggttttggt tcgcgcggcg tggaaggcaa
    10981 aatcgccgct gtgcgctatg cccgcgaaaa caacgtgcca tacttgggca tctgcctcgg
    11041 tatgcagatt gcgctgatcg aatacgcccg cgacgtggca ggtttgaaag gtgcgaattc
    11101 caccgagttc gacctcaaat gcgccgcccc cgtcgttgcc ctgattgacg aatggcaaac
    11161 tgccgacggc agcgtcgaaa cccgcgacga atccgccgat ttgggcggca ccatgcgttt
    11221 gggcgcgcaa gaggttgagt tgaaagcagg cagcctcgcc gccaaaatct acggcagcga
    11281 acacatccgc gaacgccacc gccaccgcta cgaagtcaac aacaactacg ttcctacgct
    11341 ggaacaagca ggtttggtcg tcggcggcgt atccgcagga cgcgaacgct tggtcgaaac
    11401 catcgaactg ccgaaccatc cttggttctt cgcctgccag ttccaccccg agtttacctc
    11461 caacccgcgc aaagggcatc ccttgttcac cgcgtttgtc aaagctgcgt tgaacaataa
    11521 aaaagcctga taaagcgtta cttgatgatc aaaatgccgt ctgaaagcct gacaaagact
    11581 ttcagacggc attttcgcaa gtcggggatt ataaaagcca tcaatttaat ttttatggtt
    11641 ttatctaata tttaccaact gaatattgtt aagcggggcg tgaaactcaa g
//
LOCUS       DAXHSF010000046        11627 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.046, whole genome shotgun sequence.
ACCESSION   DAXHSF010000046 DAXHSF010000000
VERSION     DAXHSF010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11627
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.046"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            39..461
                     /locus_tag="ACFM8S_000938"
     CDS             39..461
                     /locus_tag="ACFM8S_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314030.1"
                     /translation="MSAVFIAPYLTAFHEQEKTFEYAELTVTAPNRSGRAIKLDADGQ
                     HYRLSCYGFDGLCAEGNIGRTIRAEQVRAVLSETVGKGFLNGVLLEYRNSGSVYSNKD
                     FARTEDRLVEVLAQPAVFCLKLGILLLLPAIFLRLKRM"
     gene            567..1130
                     /gene="pgsA"
                     /locus_tag="ACFM8S_000939"
     CDS             567..1130
                     /gene="pgsA"
                     /locus_tag="ACFM8S_000939"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFC6314031.1"
                     /translation="MPWNLPIFLTWSRVLLIPVMTALFYLPFPWFAEETINLAAAVIF
                     AVAALTDWFDGFLARLWKQTSDFGAFLDPVADKLMVAVSLLLLVKLDRTYVLFAMIII
                     GREITISALREWMAQMGKRNSVAVATVGKFKTAAQMLAIFLLLLNIPDFYGFNLVVIG
                     NVLMFIASLLTVWSMLYYLKMAWKEIA"
     gene            1210..1285
                     /locus_tag="ACFM8S_000940"
     tRNA            1210..1285
                     /locus_tag="ACFM8S_000940"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1243..1245,aa:Gly,seq:gcc)
     gene            1311..1386
                     /locus_tag="ACFM8S_000941"
     tRNA            1311..1386
                     /locus_tag="ACFM8S_000941"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Gly,seq:gcc)
     gene            1410..1485
                     /locus_tag="ACFM8S_000942"
     tRNA            1410..1485
                     /locus_tag="ACFM8S_000942"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1443..1445,aa:Gly,seq:gcc)
     gene            1507..1582
                     /locus_tag="ACFM8S_000943"
     tRNA            1507..1582
                     /locus_tag="ACFM8S_000943"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1540..1542,aa:Gly,seq:gcc)
     gene            1608..1681
                     /locus_tag="ACFM8S_000944"
     tRNA            1608..1681
                     /locus_tag="ACFM8S_000944"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1640..1642,aa:Cys,seq:gca)
     gene            1782..1871
                     /locus_tag="ACFM8S_000945"
     tRNA            1782..1871
                     /locus_tag="ACFM8S_000945"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1816..1818,aa:Leu,seq:taa)
     gene            1975..3027
                     /gene="sohB"
                     /locus_tag="ACFM8S_000946"
     CDS             1975..3027
                     /gene="sohB"
                     /locus_tag="ACFM8S_000946"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFC6314032.1"
                     /translation="MWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSESGSVVL
                     TDFSENYKKQRQSFEAFFLSGEEAKHQEKEEKKKEKAEAKAEKKRLKEGGEKSAETQK
                     SRLFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLLRLESPGGVVHGYGLAASQL
                     RRLRERNIPLTVAVDKVAASGGYMMACVADKIVSAPFAIVGSVGVVAEVPNIHRLLKK
                     HDIDVDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPQLDIEEV
                     ATGEHWFGRQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKQSLIQRIGLQAEAS
                     VEKLFAKLVNRRADVM"
     gene            complement(3078..3944)
                     /locus_tag="ACFM8S_000947"
     CDS             complement(3078..3944)
                     /locus_tag="ACFM8S_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
                     /protein_id="HFC6314033.1"
                     /translation="MWKMLKHIAKTHRKRLIGTFSLVGLENLLMLVYPVFGGWAINAV
                     IAGKVWQALLYALVVLLMWLVGAARRIADTRTFTRIYTEIAVPVVLEQRQRQVPHSAV
                     TARVALSREFVSFFEEHLPIAATSVVSIFGACIMLLVLEFWVGVSAVGILALFLWLLP
                     RFAAISENLYFRLNNSLECDNHFIRKGDRRQLYRHYGLVARLRVLISNREAFGYLCVG
                     AAMGILFGFAFVMMTLKGYSSAGHVYSVGTYLWMFAMSLDDVPRLVEQYSNLKDIGQR
                     IEWSERNIKAGT"
     gene            complement(3954..4385)
                     /locus_tag="ACFM8S_000948"
     CDS             complement(3954..4385)
                     /locus_tag="ACFM8S_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFC6314034.1"
                     /translation="MNQLDQLGTRINLICNVFDKWIGQQDLNYNLFAVLYTLATEGSR
                     TQKHIGEEWSLPKQTVPGVCKTLAEQGLIEWQEGEQDRRERLLSLTEKGKAHAAPLTE
                     SAQEFSDKVFATFGDKRTTRLFADLDALAEVMEKTISENKK"
     gene            complement(4510..5379)
                     /locus_tag="ACFM8S_000949"
     CDS             complement(4510..5379)
                     /locus_tag="ACFM8S_000949"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFC6314035.1"
                     /translation="MSANEYTQIGWIGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAP
                     ISAKGAKVYGNTAELVRDYPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTE
                     NLAVKALVEAAGGQFAEAPVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTF
                     HFGDVGKGSGAKLVLNSLLGIFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQ
                     TKKSLWANREFPPAFALKHASKDLNLAVKELEQAGNTLPAVEIVAASYRKAVEAGYGE
                     QDVSGVYLKLAEH"
     gene            complement(5474..6391)
                     /gene="hisB"
                     /locus_tag="ACFM8S_000950"
     CDS             complement(5474..6391)
                     /gene="hisB"
                     /locus_tag="ACFM8S_000950"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HFC6314036.1"
                     /translation="MNLTKTQRQLHNFLTLAQEAGSLSKLAKLCGYRTPVALYKLKQR
                     LEKQAEDPNARGIRPSLMAKLEKHTGKPKGWLDRKHRERTVPETAAESTGTAETQIAE
                     TASAAGCRSVTVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDID
                     ISCKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLS
                     GRPGLVYNIEFTRALIGRFDVDLFEEFFHGIVNHSMMTLHIDNLSGKNAHHQAETVFK
                     AFGRALRMAVEHDPRMAGQTPSTKGTLTA"
     gene            complement(6388..7485)
                     /gene="hisC"
                     /locus_tag="ACFM8S_000951"
     CDS             complement(6388..7485)
                     /gene="hisC"
                     /locus_tag="ACFM8S_000951"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFC6314037.1"
                     /translation="MKSVRSFIRDDIQAMSAYQIADVPPGFAKLDSMESPVHPFAGHE
                     TLLQEWQARLAAAPIHLYPNPSGSGLQEALRSAFDIPDCADIALGNGSDELIQFITML
                     TAKPGAAMLAAEPSFVMYRHNAALYGMDYVGVPLNGDFTLNLPAVLEAVRKHRPALTF
                     IAYPNNPTGVCFTRAEIEAVIEASDGIVVVDEAYGAFNGDSFLPQAGSIPNLIVMRTV
                     SKIGFAGLRIGYAAGCPEVIGELQKILPPYNMNQLSLTTAKLALQHYGIISANIDSLK
                     NERERMFAELGKICHLNTFSSQANFITIRVPDADLLFDTLKQNRILVKKLHGAHPLLE
                     HCLRITVGSPAQNDAVLNIIRQLYCQPTDFL"
     gene            complement(7531..8820)
                     /gene="hisD"
                     /locus_tag="ACFM8S_000952"
     CDS             complement(7531..8820)
                     /gene="hisD"
                     /locus_tag="ACFM8S_000952"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFC6314038.1"
                     /translation="MKKLNTQSPDFQAGLKALLAFETAQNPETERIVADICADVQKRG
                     DAALIEYTNKFDQTNAKSIDDLILTQADLKAAFERIPNDVQTALQTAARRVESYHQRQ
                     KMESWSYTDEDGTLLGQQITPLDRVGIYVPGGKAAYPSSVIMNAMPAHVAGVKEIIMV
                     VPTPKGERNDIVLAAAYVAGVTKVFTVGGAQAVAALAYGTETIPQVDKITGPGNAFVA
                     AAKRRVFGVVGIDMVAGPSEILVIADGTTPADWVAMDLFSQAEHDEIAQAILIGTSQA
                     YLDEVEAAMDRLIETMPRRDIIEASLGNRGAMILAKDLDEACEIANYISPEHLELSVE
                     NPQEWAKKIRHAGAIFMGRYTGESLGDYCAGPNHVLPTSRTARFSSPLGTYDFQKRSS
                     LIQVSEQGAQKLGETASVLAHGESLTAHARAAEFRMK"
     gene            complement(8817..9716)
                     /locus_tag="ACFM8S_000953"
     CDS             complement(8817..9716)
                     /locus_tag="ACFM8S_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HFC6314039.1"
                     /translation="MVAKIKKFSDSTLSVLNNGERRFYVYCLTDLKKDKILYIGKGFG
                     NRIFEHEWVASRSQDPVSGEIIDRKLKAISKCKKLGRYIISYHLTEVEALAAESALIH
                     FVKSVLGKKLKNKIAGHGPGGISVEELDRRFGFSSLPLNEINPDGLILAIKIHNAFDL
                     DTDEELDYLFDNQDDANLKSRTLGNWVIGKDVASKVKYVIGVHTGLQNAVVSAYEVDG
                     FETMVEETKNGRKQSRYRFRTTSRSEEVLAKLGLQQKCLPELKFGSGGEKAYIRPKTE
                     TETEQENIQTTPNPKIKKEKTKS"
     gene            complement(9820..10473)
                     /gene="hisG"
                     /locus_tag="ACFM8S_000954"
     CDS             complement(9820..10473)
                     /gene="hisG"
                     /locus_tag="ACFM8S_000954"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFC6314040.1"
                     /translation="MQDNALTIALSKGRIFEETLPLLAAAGIVPTEEPEKSRKLIIGT
                     NHENIRLVIVRATDVPTYVRYGAADFGIAGKDVLIEHGGTGLYRPLDLEIAKCRMMVA
                     VRKGFDYEAASQPGCRLKIATKYPEIAASHFAGKGVHVDIIKLYGSMELAPLVGLSDA
                     IVDLVSTGNTLKANGLEAVEHIVDISSRLVVNKAALKTKYALLEPIIQAFGGAVKAK"
     gene            complement(10552..11205)
                     /locus_tag="ACFM8S_000955"
     CDS             complement(10552..11205)
                     /locus_tag="ACFM8S_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HFC6314041.1"
                     /translation="MFSVPRSFLPGVFVLAALAACKPQDNSAAQVASSSVSASAAENA
                     AKPQTRGTDMRKEDIGGDFTLTDGEGKPFNLSDLKGKVVILSFGFTHCPDVCPTELLT
                     YSDTLKQLGGQAKDVKVVFVSIDPERDTPEIIGKYAKQFNPDFIGLTATGGQNLPVIK
                     QQYRVVSAKVNQKDDSENYLVDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLL"
     gene            11482..11566
                     /locus_tag="ACFM8S_000956"
     tRNA            11482..11566
                     /locus_tag="ACFM8S_000956"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11516..11518,aa:Leu,seq:caa)
BASE COUNT         2579 a         2765 c         3271 g         3012 t
ORIGIN      
        1 atcgctgggt atggtttgcc gccataagct gtctgctgtt gtccgccgtt tttattgcac
       61 cttacctgac ggcatttcac gaacaagaaa aaacattcga atatgcggaa ttgaccgtta
      121 ccgcccccaa ccgcagcgga cgggcaatca aactggatgc agacgggcag cattaccggc
      181 tttcctgcta tgggttcgac ggtttgtgcg cggaaggcaa tatcggcaga accatcaggg
      241 cggagcaggt tagagccgtt ttaagcgaaa ccgtcggcaa aggtttttta aacggcgttc
      301 tgttggaata ccgcaacagt ggcagtgtct atagcaataa agattttgcc cgcacggaag
      361 accgccttgt cgaagtgttg gcacaacctg ccgttttctg cctgaaactg ggtattttgc
      421 ttttattgcc cgccattttc ctgcgtttga aaagaatgtg aaaacagatt ggcggggcgg
      481 gggaatgccc atgcagcaat tttacgtttt gggttatgag gataaccgtt ataatcacaa
      541 ctttacagtc tgcacagaga aaaccgatgc cttggaatct ccccatcttc ctcacatggt
      601 cgagggtctt gctcattcct gtcatgacag ccctttttta cctgcctttt ccgtggttcg
      661 cggaggaaac gattaatctc gccgccgccg ttatttttgc cgttgccgcc ttaaccgact
      721 ggtttgacgg atttttggca agactgtgga aacagacttc cgatttcggc gccttcctcg
      781 atcccgtcgc cgacaagctg atggttgccg tatcgctact tttactggtc aaactcgacc
      841 ggacctatgt tttgtttgcc atgattatca tcggcaggga aattaccatt tccgcattgc
      901 gcgaatggat ggcgcaaatg ggcaaaagga acagcgttgc cgtcgccacc gtcggtaagt
      961 tcaaaaccgc cgcgcaaatg ctggcaatct tcctgctgct gctgaatatt cccgattttt
     1021 acggatttaa tcttgttgtt atcggcaatg tattgatgtt tatcgcatct ttgctgacag
     1081 tctggtcgat gctgtattat ctgaaaatgg cgtggaaaga aatcgcctga aaaaaacata
     1141 aaaatagctt gacggcaaaa acagaatcca taataattgc gtctcttcga tgttggagaa
     1201 tgaaatcggg cgggaatagc tcagttggta gagcgcaacc ttgccaaggt tgaggtcgcg
     1261 agttcgagac tcgtttcccg ctccaaaaat ttcattctcc cacaatcatt gcgggaatag
     1321 ctcagttggt agagcgcaac cttgccaagg ttgaggtcgc gagttcgaga ctcgtttccc
     1381 gctccaagtt ttatttcaaa cgtatcaacg cgggaatagc tcagttggta gagcgcaacc
     1441 ttgccaaggt tgaggtcgcg agttcgagac tcgttttccg ctccaaaaac cgatatgttt
     1501 gaacgcgcgg gaatagctca gttggtagag cgcaaccttg ccaaggttga ggtcgcgagt
     1561 tcgagactcg tttcccgctc caagtttttt agataagcca gttttagggc gagatagcaa
     1621 agtggttatg cagcggattg caaatccgtc tacgccggtt cgattccgac tctcgcctcc
     1681 atatatatta cggcggggtg gcagagtgtt tatgctatga ggagtgcaat cttcatatag
     1741 gccggttaaa atccgcgccc ccgcctccac ctttcacaaa tgcccgggtg gtgaaatagg
     1801 tagacacaac ggacttaaaa tccgtcggga ctaaacatcc cgtgccggtt cgattccggc
     1861 tccgggcacc aagctgaaaa tgacaatacc gctccaagcg gttatttttt tatctgtcgg
     1921 acggtgatgg acaatattga catgttcatg cctgaacaag aggaaatcca atcaatgtgg
     1981 aaagaaattt tactgaatta cggtattttc ctgctcgaac tgcttaccgt gttcggcgca
     2041 attgcgctga ttgtgttggc tatcgtacag agtaagaaac agtcggaaag cggcagtgtc
     2101 gtactgacgg atttttcgga aaattataaa aaacagcggc aatcgtttga agcattcttt
     2161 ttaagcgggg aagaggcaaa acatcaggaa aaagaggaaa agaaaaagga aaaggcggaa
     2221 gccaaagcag agaaaaagcg tttgaaggag ggtggggaga aatctgccga aacgcaaaaa
     2281 tcccgccttt ttgtgttgga ttttgacggc gatttgtatg cacacgccgt agaatccttg
     2341 cgtcatgaga ttacggcggt gcttttgatt gccaagcctg aagatgaggt tctgcttaga
     2401 ttggaaagtc cgggcggcgt ggttcacggt tacggtttgg cggcttcaca gcttaggcgt
     2461 ttgcgcgaac gcaatattcc gttgactgtt gctgtcgata aggtggcggc aagcggcggt
     2521 tatatgatgg cgtgtgtggc ggataaaatt gtttccgctc cgtttgcgat tgtcggttcg
     2581 gtgggtgtgg tggcggaagt gccgaatatc caccgcctgt tgaaaaaaca tgatatcgat
     2641 gtggatgtga tgacggcggg cgaatttaag cgcacggtta cctttatggg tgaaaatacg
     2701 gaaaagggca aacagaaatt ccgacaggaa ctggaggaaa cgcatcagtt gttcaagcag
     2761 tttgtcagcg agaaccgccc tcaattggat attgaggaag tggcaacggg cgagcattgg
     2821 ttcggtcggc aggcgttggc gttgaacttg attgacgaga tttcgaccag tgatgatttg
     2881 ttgttgaaag cgtttgaaaa caaacaggtt atcgaagtga aatatcagga gaagcaaagc
     2941 ctgatccagc gcattggttt gcaggcggaa gcttctgttg aaaagttgtt tgccaaactt
     3001 gtcaaccggc gggcggatgt gatgtagttt gccgaacgga tggaaatgcc gtctgaagcg
     3061 tgttcagacg gcatttttca agttccggct ttgatgttcc gttccgacca ctctatccgt
     3121 tgtccgatgt ctttcaaatt ggaatattgt tcgaccaatc gcggcacgtc gtccaaactc
     3181 atggcaaaca tccacagata agtgccgacc gaatagacat gccccgcgct gctgtagcct
     3241 ttgagcgtca tcatcacaaa agcaaagccg aacaaaatac ccatcgccgc gccgacgcag
     3301 agatagccga aggcttcgcg gttggaaatc agcacacgca ggcgcgcaac cagtccgtaa
     3361 tggcggtaca gctgccgtcg gtctcctttt cggataaagt ggttgtcgca ttccaagctg
     3421 ttgttcaggc ggaaatacag gttttcgcta atggcggcaa aacgtggcaa aagccataaa
     3481 aacaacgcaa gtatgcccac cgccgacacg ccgacccaaa attccagcac cagcagcatg
     3541 atgcacgcgc cgaatatgga tacgacggat gtcgcggcaa tcggcaggtg ttcttcaaaa
     3601 aagctgacaa actcacgcga cagggcaact cgcgcagtta ccgccgaatg cgggacttgt
     3661 cgctgccgct gttccaacac gaccggcacg gcgatttcgg tataaatccg cgtaaacgtg
     3721 cgcgtatcgg caatccgccg cgccgcaccg accagccaca tcaaaagcac aaccaaagcg
     3781 tacagcaacg cctgccacac cttccccgca attacggcat taattgccca gccgccaaac
     3841 accggataca ccagcatcaa aaggttttcc agtccgacca gggaaaatgt gccaatcaat
     3901 cgcttgcggt gggttttggc tatgtgtttc aacattttcc acatctttgc tccctatttt
     3961 ttattttccg agattgtttt ttccatcact tccgccagtg catccaaatc ggcaaacagc
     4021 cgagttgtgc gcttgtcgcc gaatgtggca aatactttgt cgctgaattc ctgcgcgctt
     4081 tctgttaaag gtgcggcatg ggctttgcct ttttcggtca gcgacagcaa ccgttcgcgc
     4141 cggtcctgtt cgccttcctg ccattcaatc aacccttgtt cggcaagggt tttgcatacg
     4201 cctggaacgg tctgtttggg caaactccac tcttcgccga tatgcttttg cgtgcggctg
     4261 ccttcggttg ccagggtata aagtacggca aagaggttgt aattcaaatc ctgctgcccg
     4321 atccatttgt cgaagacatt gcaaatcagg ttgatacggg tgccaagttg gtcgagttgg
     4381 ttcataattg gtcttgatat tgactaaatt tgtgcaggtt atagtcataa tcgggactaa
     4441 ttttaagcgt ggggcgataa aaatgccgtc tgaaaatatt tcagacggca ttgtgtttgg
     4501 aaaaggcaat cagtgttctg ccaatttcag gtaaacgccg gaaacgtcct gttcgccgta
     4561 gccggcttcg actgctttgc ggtagctggc agcaacgatt tcgacggcgg gcagggtgtt
     4621 gcctgcctgt tcaagctctt tgacggcgag gttgaggtct ttggaggcgt gtttgagggc
     4681 gaaggcgggt gggaattcgc ggtttgccca cagggatttt ttggtttgga acatgggcga
     4741 gtccattgcc gagccgccga tggcttcgac gatggtgtcg gtatcgatgc cgaactgccg
     4801 cgccatcagc atcgcttcgc tgtacgcttc gccgaaaatg cccaagagcg agttcaagac
     4861 gagtttcgcg cccgaacctt tgccgacatc gccgaaatgg aaggtttttt tgccgacgag
     4921 ggaaaatatt ttttgcagcg ggtttaaaac ggcttcgctg ccgccgaaca gaatcagcag
     4981 cgtgccgttg gtggcgggcc cgaccgatcc ggaaacgggt gcttcggcaa actgtccgcc
     5041 tgcggcttcg acaagtgctt tgacggcgag gttttcggtc ggggagatgg tgctcatgtt
     5101 gacgatgatt ttgccggcca atccgtcgcg gactccgttc aggatgtcgc acacggcggc
     5161 atagtcggaa accatcagga aaatgacggg atagtcgcgg acgagttcgg cggtgttgcc
     5221 gtaaactttt gcgcctttgg cggagatggg ggcagttttg tcgggcgagc ggttgtatac
     5281 gccgacttcg atgccgccgt ccaagagccg cgttaccata ggcagaccca tttgccctaa
     5341 gcctatccag ccgatttgtg tgtattcgtt tgcggacatg atgtttactc cttttctgtt
     5401 tgtagtgtag tgtaatctta tctgttccga taccgtctga aaagcctgcg ggtgtttcag
     5461 acggtatggt tttttatgcg gtcagcgtgc ctttggtcga gggggtctgt cctgccatgc
     5521 gcgggtcgtg ttcgactgcc atacgcaggg cgcgcccgaa ggctttgaat acggtttccg
     5581 cctgatggtg ggcgtttttg ccgctgaggt tgtcgatgtg cagggtcatc atactgtggt
     5641 tgacgatgcc gtggaaaaat tcttcaaaca aatcgacatc gaaacgtccg attagtgcgc
     5701 gggtaaattc gatgttgtac acgagtccgg ggcggccgga aaggtcgatg acgacgcggc
     5761 tgagggcttc gtcgagcggg acgtaggaat gtccgtaacg gcggatgcct tttttgtcgc
     5821 cgagtgcctg ccggattgct tgtccgagtg tgatgccgat gtcttcggcg gtgtggtggt
     5881 cgtcgatgtg caggtcgcct ttgcagctga tgtcgatgtc aatcatgccg tggcgggcga
     5941 tttgatcgat catgtgttcg aggaagggta cgccggtatc cagcctgctt ttgccgctgc
     6001 cgtcgaggtt gatggagacg gtgatttggg tttcgcaggt attgcggttg acggtaacgc
     6061 tgcggcagcc ggcagcagat gcggtttcgg caatttgggt ttcggcagtt ccggtgcttt
     6121 ctgcggcggt ttcggggaca gtgcgttcgc ggtgttttct gtcgagccag cctttgggtt
     6181 tgccggtgtg tttttcgagt tttgccatca ggctgggacg gatgccgcgt gcatttgggt
     6241 cttctgcctg cttttcaagg cgttgtttga gtttgtagag tgcgacgggg gtacggtagc
     6301 cgcagagttt ggcgagcttg gacagcgaac ctgcttcttg ggcgagggtc agaaagttgt
     6361 gcagttggcg ttgtgtttta gtcaaattca taggaaatcc gttggttggc agtaaagttg
     6421 gcgaatgatg ttgagaacgg catcgttttg tgcggggctg cctacggtaa tgcgcaggca
     6481 gtgttccaaa agcgggtgcg cgccatgcag ttttttaacc aagatgcggt tttgtttgag
     6541 cgtgtcaaac aacaaatcgg catcgggtac gcgtatggta atgaagtttg cctgacttga
     6601 aaaggtgttc agatggcata ttttgcccaa ttcggcgaac atccgttcgc gttcgttttt
     6661 caggctgtcg atgttggcag agatgatgcc gtagtgttgc agggcgagtt tggcggtggt
     6721 caggctcagt tggttcatat tgtagggcgg caggattttt tgcagttcgc cgatgacttc
     6781 gggacagcct gccgcatagc cgatacgcag tccggcaaaa ccgattttgc tgacggtacg
     6841 catgacgatg aggttgggaa tgctgcccgc ctgcggcagg aagctgtcgc cgttgaatgc
     6901 gccgtaggct tcgtcgacga cgacgatgcc gtctgaagct tcgatgacgg cttcgatttc
     6961 ggcacgcgtg aaacatacgc cggtggggtt gttggggtag gcgataaagg tcagggcagg
     7021 gcggtgcttc ctgacggctt cgaggacggc gggcaggttg agggtgaaat ctccgttcag
     7081 tggaacgccg acataatcca tgccgtacag cgcggcgttg tggcggtaca tgacgaaact
     7141 gggttcggct gccaacattg ccgcgcccgg tttggcggtc agcatcgtga tgaactgtat
     7201 cagctcgtcc gagccgttgc ccagcgcgat gtcggcgcag tcgggaatgt cgaacgccga
     7261 acgtaatgct tcctgtaaac cgctgccgga gggattgggg taaagatgga tgggcgcggc
     7321 ggcaagccgt gcctgccatt cctgcaacag cgtttcatgt ccggcaaaag ggtggacggg
     7381 actttccatc gaatcgagtt tggcaaagcc gggcggaacg tcggcaatct gatatgccga
     7441 catagcttgt atgtcgtcgc ggatgaagga gcggacggat ttcatcgtgt ttccttaatg
     7501 gttggaatat gcctgtacgc cgtttcggca ttatttcata cggaactctg ccgcgcgggc
     7561 gtgggcggtc aggctttcgc cgtgtgccag cacgctggcg gtttcgccta atttttgcgc
     7621 gccctgttcc gaaacctgaa tcaggctgga gcgtttttgg aaatcatatg tccccaaagg
     7681 cgaggaaaag cgggcagtgc ggctggtggg caatacatgg tttggaccgg cgcagtagtc
     7741 gccgaggctt tcgccggtgt agcgtcccat gaaaatcgcg ccggcgtggc ggattttttt
     7801 cgcccattcc tgcggatttt cgactgacaa ttccaagtgt tcgggggaaa tgtagttggc
     7861 gatttcgcag gcttcgtcca agtctttggc gagtatcatc gcgcccctgt tgccgagcga
     7921 ggcttcgatg atgtcgcggc gcggcatggt ttcgatgagc ctgtccatag cggcttctac
     7981 ttcgtcgaga tacgcttgcg acgtgccgat taagatggct tgggcaattt cgtcgtgttc
     8041 ggcctggctg aacaaatcca tcgccaccca atcggcaggt gtcgtgccgt cggcgatgac
     8101 caggatttca gacggccccg ccaccatgtc gatgccgacc acgccgaaca cgcggcgttt
     8161 ggcggcggcg acgaaggcgt tgcccggacc ggtgatttta tcgacttggg ggatggtttc
     8221 cgtgccgtag gcgagggcgg caaccgcctg cgcaccgccg acggtgaaga ctttggttac
     8281 gccggcgacg tatgcggcgg caagtacgat gtcgttgcgt tcgccttttg gtgtcggcac
     8341 gaccatgatg atttctttca cacctgcgac gtgggcgggc atggcgttca tgatgacgga
     8401 actcggatac gccgccttgc cgccggggac gtaaatgccg acgcggtcaa gcggtgtgat
     8461 ttgttgtccc aacagcgtgc cgtcttcatc ggtgtagctc cacgattcca ttttttggcg
     8521 ttggtggtag ctttcgacac ggcgggcggc ggtctgcaat gccgtctgaa cgtcgttcgg
     8581 aatgcgctcg aacgccgctt tcaaatcggc ttgcgtgagt attaaatcat cgatgctttt
     8641 agcgtttgtc tgatcgaatt tgttggtgta ttcaatcaaa gccgcatcgc cgcgcttttg
     8701 cacgtcggcg caaatgtcgg cgacgatgcg ttcggtttcg gggttttgcg cggtttcaaa
     8761 agccagcagg gctttgagtc cggcttggaa atcgggcgat tgggtgttga gttttttcat
     8821 gatttggttt tttccttttt tatttttgga ttgggggtcg tctgaatatt ctcttgttca
     8881 gtttctgtct ctgttttggg tctgatatac gctttttctc ccccgctacc aaacttcaat
     8941 tcgggcaggc atttttgttg cagaccgagt ttggctaata cctcttcgct acgagaggta
     9001 gtgcggaaac ggtaacggga ttgttttcta ccgtttttgg tttcctcaac cattgtttca
     9061 aaaccgtcca cttcgtatgc actgacaaca gcgttttgca gaccggtgtg aacgccgata
     9121 acgtatttca cttttgaagc aacatcttta ccgataaccc agttgcccaa cgtacgcgat
     9181 ttgaggttgg catcgtcttg gttgtcgaaa aggtagtcta attcttcgtc agtatctaaa
     9241 tcgaaagcat tgtggatttt gatggcgaga atcagcccgt cggggttaat ctcgttaagt
     9301 gggagagaag agaatccaaa gcggcgatct agttcttcta cgctaatacc acccggacca
     9361 tgcccggcaa ttttattttt gagtttttta cccaagacag atttaacaaa atgaattaag
     9421 gcagattcgg cggcgagtgc ttcgacttca gtcagatgat agctgatgat atagcgaccg
     9481 agtttcttgc atttggagat ggctttgagt ttccgatcga taatctcgcc ggagactggg
     9541 tcttgtgaac gactagcaac ccattcatgc tcgaagatac gattaccgaa gcctttgccg
     9601 atgtagagga ttttgtcttt tttcaggtcg gtcagacaat agacataaaa ccgacgctcg
     9661 ccgttattca aaacggaaag ggttgaatct gagaattttt ttatttttgc aaccatttta
     9721 acttctccta tacagtttac tgccggtgat attttcagaa gacctgtcgg tgggaacgga
     9781 tagggtcgtc tgaaaacgca tctttattca aatgaatgct tacttcgcct tcactgcgcc
     9841 gccgaacgcc tgaataatcg gctccagcag cgcgtatttc gttttcaaag cagccttgtt
     9901 gaccaccagg cggctggaaa tgtcgacgat gtgttcgact gcctccaagc cgtttgcctt
     9961 caaggtgttg cccgtcgaaa ccaagtccac aatcgcatcg ctcaaaccga ccagcggcgc
    10021 aagttccatc gagccgtaca gtttgataat gtccacatgg acacccttgc cggcaaaatg
    10081 agatgccgcg atttcaggat acttcgtggc aatcttcaga cggcatccgg gttgcgaagc
    10141 tgcttcgtaa tcaaaccctt tacgcacagc aaccatcatg cggcacttgg caatctccaa
    10201 atccaaaggc cggtaaagcc ccgtgccgcc gtgttcgatc agcacgtctt tgcccgcaat
    10261 gccgaagtcc gccgcgccgt agcggacata agtcggcaca tcggttgcgc ggacaatgac
    10321 aaggcggatg ttttcatggt tcgtcccgat aatcagcttg cgcgattttt caggctcttc
    10381 agtcggaaca atgccggcag cggcaagcag cggcagcgtc tcctcaaaaa tgcgcccctt
    10441 ggataaggcg atggtcaaag cattatcctg catggtgtgg atgtcctttg tcggttgaaa
    10501 caataatgcc gtctgaatag gcgccgacgg tgcagacggc atacggtttt atcagagcag
    10561 agtccttaca tcggcagcaa tcgtttctgg ctcgcttccg taaggcgaga aaatggcaac
    10621 ctcaccgttt ttgtcgatga gatacgcacc ggaagagtgg tcgaccaaat agttttcgct
    10681 gtcgtctttt tgattgactt tggcagaaac cacgcggtat tgctgcttga tgaccggcag
    10741 gttttggccg cccgttgccg tcagaccgat aaagtccgga ttgaactgtt tggcatactt
    10801 gccgatgatt tcaggcgtgt cgcgttccgg atcgatgctg acgaacacca ctttcacgtc
    10861 cttagcctgc ccgcccaact gcttcaacgt gtcgctgtac gtcaaaagct ctgtcgggca
    10921 gacatcggga cagtgcgtaa agccgaaaga cagaatcacg accttgcctt tcaaatcgct
    10981 caggttgaaa ggcttgcctt cgccgtcggt cagcgtgaaa tcgccgccga tgtcttcctt
    11041 acgcatatcc gtaccgcgcg tttgcggctt tgccgcattt tccgcagccg acgcggatac
    11101 acttgaagaa gcgacttgcg ccgcactgtt gtcttgaggt ttgcaggcgg caagcgcggc
    11161 aagtacgaaa acgcccggca aaaaggaacg cggtacggaa aacatgacaa tctcccaaga
    11221 ataaatagca aaacctgatt cgtacggaat ttgaatattt ccatcttaac gcgtttggca
    11281 gatttaatgt agtatttttt tacgttgtct gcacggcagg aattgccgaa tcatacagaa
    11341 tctttggagc ggaaagaaca gtaagattaa aataataaaa attctattta aaatcaaaaa
    11401 taaaataaaa acagataaaa taatttcaaa aaaaaggttt acagaatttc aaaagccatt
    11461 ataatgacga cttcttcacc agcccaggtg gcgaaattgg tagacgcagg ggactcaaaa
    11521 tcccccgccg caaggtgtgt cggttcgagt ccgaccctgg gcaccacaac cgcttttaga
    11581 gcggttattt ttttgtctat tgctgtttaa tgaaatagat aagacta
//
LOCUS       DAXHSF010000047        11610 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.047, whole genome shotgun sequence.
ACCESSION   DAXHSF010000047 DAXHSF010000000
VERSION     DAXHSF010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11610
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.047"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(175..867)
                     /locus_tag="ACFM8S_000957"
     CDS             complement(175..867)
                     /locus_tag="ACFM8S_000957"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFC6314042.1"
                     /translation="MERFTYTLSDGLCIEIELKRSAKKNLILRPVNMQTVSINVPPFF
                     QDHALANWLAANETILRNTLAKTPVHPVSHPNLPEWIWYRGIKTKLDTHSQSHIRITS
                     SEILLPRKETAAQIDHLRRLLNERAREYLLPRLEKHAAETGLTPTATDLSNAKTFWGV
                     CRPHTGIRLNWRLIGAPKYVADYVCIHELCHLRHPDHSPRFWHLVNTLTPHTDNAKSW
                     LKAHGRELFVLG"
     gene            complement(975..2168)
                     /locus_tag="ACFM8S_000958"
     CDS             complement(975..2168)
                     /locus_tag="ACFM8S_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HFC6314043.1"
                     /translation="MSNQKNRRNALIGAAFLMATSAIGPGFLTQTATFTQALAASFGF
                     VILLSILLDIGAQLNIWRIVAVSEKQAQDIANQVLPGAGYFLAVLIVMGGLAFNIGNV
                     GGAGLGLNLLTGLSPETGAVISGVIAIGVFLFKEAGKVMDKFAQVMGFVMIALTVYVA
                     WQANPPLADAAVHTFMPEKLDAMAIVTLVGGTVGGYITFAGAHRLLDAGIKGKSALPE
                     VSQSSVRAILIASIMRIVLFLAVLGVVSQGVQLNPDNPASTPFEYAAGYIGLLIFGVV
                     IWAASITSVIGAAYTSVSFFSGLSPSIERNKNKWIIAFIAVSTAVFSTIGKPAQVLVF
                     VGALNGLILPISLGLILIAAYKTKIVGDYKHPLWLTVSGVIVVGLMAVLSAITISKYI
                     GGLFG"
     gene            complement(2297..3034)
                     /gene="pxpA"
                     /locus_tag="ACFM8S_000959"
     CDS             complement(2297..3034)
                     /gene="pxpA"
                     /locus_tag="ACFM8S_000959"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFC6314044.1"
                     /translation="MKQVDLNADLAEGCGSDEALLQLITSANIACAQHAGSIADIRAA
                     LAYAQQNGVRIGAHPGYPDRENFGRTEMNLSEADLRACLNYQLGALQALCRDQGLEMA
                     YVKPHGAMYNQAAKNRALADTVARIVADFDPKLKLMALSGSLLLEAGKAAGLGVISEV
                     FADRRYMPDGTLVPRSRPDAQVDSDEEAIAQVLQMVRDGRVKAVNGSLVAVQADSICL
                     HGDGPHAVVFAEKIRQELLAAGIKVSA"
     gene            complement(3021..3950)
                     /locus_tag="ACFM8S_000960"
     CDS             complement(3021..3950)
                     /locus_tag="ACFM8S_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HFC6314045.1"
                     /translation="MIHVSAVQAPAHIQDTGRYGHRRYGIGHAGAMDTVALAAGNILL
                     GNDEGTAAIEIALGGIMLVFERDTPFCLTGAVYQAELDGEPVYSYWRYTARKGQTLKM
                     VRAVQGMYGYVCVAGGFDVPEVMGSRSTDLKAGFGGHQGRMLQKGDCLPIGKGAQELS
                     KVGIAPIPFTDTVHLVPSSEYAAFSEKGRLNLERETWTLQSDSNRMGYRFDGQPLTLS
                     QPLEMLSHAVQAGTVQVPPGGKPIILLADAQTTGGYPKIATVAAADLGRLAQVRFGSK
                     VKFKIIGLKEATALRRKNQVYLNQIRRITHEAG"
     gene            complement(3947..4594)
                     /gene="pxpB"
                     /locus_tag="ACFM8S_000961"
     CDS             complement(3947..4594)
                     /gene="pxpB"
                     /locus_tag="ACFM8S_000961"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFC6314046.1"
                     /translation="MRIEITPISESALVCRLNAPSELGKQQKLWAFAAALGQHDRIEE
                     VVVGMNNLTVFTRFDTDLATLADELQYVWEHTAVTDHQGKLVEIPVCYGGEYGPDLAE
                     VAAFHQTVISEIVRRHTAQTYTVFMMGFQPGFPYLGGLPEALHTPRRAVPRTSVPAGS
                     VGIGGSQTGVYPFASPGGWQIIGRTELPLFRADLNPPTLLAAGDQVRFVAERIEP"
     gene            4778..6985
                     /gene="uvrD"
                     /locus_tag="ACFM8S_000962"
     CDS             4778..6985
                     /gene="uvrD"
                     /locus_tag="ACFM8S_000962"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFC6314047.1"
                     /translation="MFPDQSAPNLLQGLNPEQLSAVTWPPQSALVLAGAGSGKTRVLT
                     TRIAWLLQSGQASVHSIMAVTFTNKAAKEMQTRLGAMIPVNVRAMWLGTFHGLCHRFL
                     RLHHRDAGLPSSFQILDSGDQLSLIKRLLKSLNIAEEIIAPRSLQGFINAQKESGLRA
                     SVLSAPDPHTSRMIECYAEYDKICQREGVVDFAELMLRSYEMLQSNEILRQHYQNRFN
                     HILVDEFQDTNKLQYAWLKLMAGGNAAVFAVGDDDQSIYRFRGANVGNMTALMEEFHI
                     DAPVKLEQNYRSVGNILAAANAVIENNDERLGKNLRTDAEAGDKIRYYSAFTDLEEAR
                     FIVDETKALEREGWDLDEIAVLYRSNAQSRVIEQSLFRSGIPYKIYGGLRFYERQEIK
                     HALAYLRLAVNPDDDNALLRVINFPPRGIGARTVENLQTASQEQGVSLWQAACNAGAK
                     AAKVAAFVRLIEALRNQVGQMPLPEIIAGILKDSGLTEHYRTQKGDNQDRLDNLDELV
                     NAAIEFKPEDSNFETLPDNISDDPAFPILAFLSNAALESGENQAGAGEKAVQLMTVHA
                     AKGLEFNAVFLTGMEEGRFPSEMSLAERGGLEEERRLMYVAITRARKRLYITMAQQRM
                     LHGQTQFGIVSRFVEEIPPEVLHYLSVKKTAYDSYGSPRQTAASKDKIIDDYKQPQTY
                     AGFRIGQNVRHAKFGTGVIIDAADKGESARLTINFGKQGVKELDTKFAKLEAM"
     gene            7342..7881
                     /locus_tag="ACFM8S_000963"
     CDS             7342..7881
                     /locus_tag="ACFM8S_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HFC6314048.1"
                     /translation="MSQVFKDFDLSSVWESDSWADENYKEAPFTPEILAAVESELGYK
                     LPQSFIELMAVQNGGIFVKNCFPTTQRNSWAKDHVQICEVSGIGFEKEGSLCGETGQK
                     LWMEEWEYPPIGVYFANDPSGGHAMFALDYRECGKDGEPKVVFVEQESDFEIVELAPD
                     FETFIRSLRHEDEFIDEEI"
     gene            complement(8071..8286)
                     /locus_tag="ACFM8S_000964"
     CDS             complement(8071..8286)
                     /locus_tag="ACFM8S_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314049.1"
                     /translation="MNIINYTGDDIIISLTREELQLLRSLVIEIYAGVCIDAEEFEIV
                     SGIRNSKLVQELEQQLIEAYDLMDTKG"
     gene            complement(8410..8769)
                     /locus_tag="ACFM8S_000965"
     CDS             complement(8410..8769)
                     /locus_tag="ACFM8S_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HFC6314050.1"
                     /translation="MIKLDLKSINLYDIDFEKFTPEIPDNFHRWIDLDIGIEGEQGSS
                     IFSLCICSPKWISHHCNKEGFFWSNALILEQFDHKIIKSEIDKILEYCSKETWDLTLS
                     NLLRFFSWEFEDYNPNT"
     gene            8822..9199
                     /locus_tag="ACFM8S_000966"
     CDS             8822..9199
                     /locus_tag="ACFM8S_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC6314051.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTATEPDSIVYTDCYRSYDV
                     LDVSEFSHFRINHSTHFAERQNHINGIENFWNRAKRHLRKFNGIPKAHFELYLKECEW
                     RFNNSEIKVQISILKQLVKSSLS"
     gene            9398..10018
                     /locus_tag="ACFM8S_000967"
     CDS             9398..10018
                     /locus_tag="ACFM8S_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HFC6314052.1"
                     /translation="MNSTASKTLKGLSLVFFASGFCALIYQVSWQRLLFSHIGIDLSS
                     ITVIISVFMVGLGVGAYFGGRIADRFPSSIIPLFCIAEVSIGLFGLVSRGLISGLGHL
                     LVEADLPIIAAANFLLLLLPTFMMGATLPLLTCFFNRKIHNVGESIGTLYFFNTLGAA
                     LGSLAAAEFFYVFFTLSQTIALTACFNLLIAASVWLRYRKDGYSEH"
     gene            10041..11492
                     /locus_tag="ACFM8S_000968"
     CDS             10041..11492
                     /locus_tag="ACFM8S_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HFC6314053.1"
                     /translation="MLSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIA
                     VGAYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLS
                     AVVRGLIFPLVHHVGTDGNKSGRQVSNVYFANVAGSALGPVLIGFVILDFLSTQQIYL
                     LICLISAAVPLFCTLFQKSLRLNAVSVAVSLMFGILMFLLPDSVFQNIADRPDRLIEN
                     KHGIVAVYHRDGDKVVYGANVYDGAYNTDVFNSVNGIERAYLLPSLKSGIRRIFVVGL
                     STGSWARVLSAIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLR
                     RHPDEKFDLILMNTTWYWRAYSTNLLSAEFLKQVQSHLTPDGIVMFNTTHSPHAFATA
                     VHSIPYAYRYGHMVVGSATPVVFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKV
                     VSRMLIQMTEPSAGAEVITDDNMIVEYKYGRGI"
BASE COUNT         2848 a         3079 c         2839 g         2844 t
ORIGIN      
        1 cctctttcag ttacagcagc ttgatccctt tcccttatcc aacgggggaa ggctaggata
       61 gggtggcttg caaatataca gaacaaggga caagagccac cctctctcca accctctccc
      121 tccgtacggg agggggtgga ttctcgcggg cgaagcccac gctacggtta gcctttaccc
      181 cagcacaaac aattcccgcc cgtgcgcctt cagccaactt ttagcattgt cggtatgcgg
      241 cgtcagcgtg ttcaccaaat gccaaaagcg cggactgtgg tcggggtggc ggaggtggca
      301 gagttcgtgg atgcagacat agtcggcaac gtacttgggc gcgccgatca gccgccagtt
      361 gaggcggatg ccggtgtgcg ggcggcatac gccccaaaag gttttggcgt tgctcaggtc
      421 tgtggcggtg ggcgtcagtc ctgtttcggc tgcgtgtttt tcaaggcggg gcagcaggta
      481 ttcgcgggcg cgttcgttca acaggcggcg caggtggtcg atttgtgcgg cggtttcttt
      541 tcggggaagc aggatttcag acgacgtgat acggatatgg ctttggctgt gggtatccag
      601 cttggtcttt attccccgat accaaatcca ctcgggtaag tttgggtggg aaacaggatg
      661 cacgggcgtt ttggcaagcg tgttccgcaa aatcgtttcg tttgccgcca gccagtttgc
      721 taacgcgtgg tcttgaaaaa agggtgggac gttgatgctg accgtctgca tattgacggg
      781 gcgcagaatc agatttttct tggcactgcg tttgagttcg atttcgatgc acaaaccgtc
      841 ggaaagagta taggtgaagc gttccatagt tgtgaatagg tttcagaccg gatacatcgt
      901 ctgaaacagg aattttccat atcaggcggc aaacttcgga taatatacaa aatcaaacat
      961 ctgcgctaca aggttcagcc gaacaagccg ccgatatatt tgctgatggt aatggcgctg
     1021 agtactgcca tcaaaccgac cacaatcacg ccggaaacgg tgagccacag cgggtgtttg
     1081 tagtcgccga caattttggt tttgtaggcg gcaatcagaa tcagaccgag ggaaatcggt
     1141 aaaatcaggc cgtttaatgc gcctacgaac accagcacct gcgccggttt gccgatggtg
     1201 gaaaatacgg cggtggacac ggcgataaag gcaataatcc atttgttttt attgcgttcg
     1261 atagacgggc tgagaccgga gaagaacgac accgaagtat aagccgcacc aatcaccgaa
     1321 gtaatcgaag ccgcccaaat caccacgccg aaaatcagca ggccgatgta tcccgccgca
     1381 tattcaaacg gtgtggaagc agggttgtcg ggattgagct gtacgccttg gctgaccacg
     1441 cccaaaaccg ccaaaaacaa tacaatccgc ataatcgagg caatcaggat cgcccgcacc
     1501 gagctttggc tcacttccgg caacgccgat ttgcctttga tgcctgcgtc cagcagacgg
     1561 tgcgcaccgg cgaaggtgat gtagccgccg accgtgccgc ccaccagtgt aacaatcgcc
     1621 attgcatcga gtttttccgg cataaaggta tgcacggcgg catctgccag cggcggattc
     1681 gcctgccatg ccacataaac cgtcagcgca atcattacga aacccatcac ttgggcgaat
     1741 ttgtccatca ctttgcctgc ttctttaaac agaaacacac cgatggcaat cacgccgctg
     1801 atcacggcac cggtttccgg tgacagtccg gtcagcaggt tcagacccaa gcctgcgccg
     1861 ccgacgttgc caatattgaa cgccaaaccg cccatcacaa tcagcacagc caagaaatag
     1921 cctgcgccgg gcaagacctg attggcaata tcctgcgcct gtttttcgga aacggcgaca
     1981 atccgccaaa tattgagctg cgccccgatg tcgagcagaa tcgagagcag aatcacaaag
     2041 ccgaaacttg ccgccagtgc ttgggtgaag gtggcggttt gggtcagaaa gcccgggccg
     2101 atggcggaag tcgccatcag gaatgcagcg ccgattaagg catttctgcg gtttttttga
     2161 ttagacataa tcgcttatcc tctataaaat tggttgttgc tgtgtttggg cgaaacctgc
     2221 ggttttagct acgcagaaac tcgctttgct cgttttggcg aaacctgcgg ttttcagacg
     2281 gcctatgaac tgtttttcaa gcagaaactt tgatgcctgc cgccagtagt tcctgccgga
     2341 ttttttcggc aaacaccacg gcgtgcggcc cgtctccgtg cagacagatg ctgtcggctt
     2401 gcacggcaac caggctgccg ttcactgctt tgacccgccc gtcccgcacc atctgcaata
     2461 cttgggcgat ggcttcttcg tcgctgtcca cctgcgcatc ggggcggctg cggggaacca
     2521 gcgtaccgtc gggcatatag cggcggtcgg cgaatacttc ggaaatcaca cccaagcctg
     2581 cggcttttcc ggcttccaag agcaggctgc cggaaagtgc catcaatttc aatttcgggt
     2641 cgaaatccgc cacaattcgg gcaacggtat ccgccagcgc acggtttttc gccgcttggt
     2701 tgtacattgc gccgtgcggt ttgacataag ccatttccaa accctgatca cggcacaagg
     2761 cctgcaatgc gcccaactgg taattcagac acgcccgcaa atcggcttcg gacagattca
     2821 tttcggtacg gccgaagttt tcccgatcgg gatagccggg gtgtgctccg atgcgcacgc
     2881 cgttttgttg ggcatacgcc aatgccgccc gaatatcggc aatgctgccg gcgtgttggg
     2941 cgcaggcgat gttggccgaa gtaatcagct gcaacaaggc ttcgtcgctg ccgcagcctt
     3001 cggcgagatc ggcgtttaaa tcaacctgct tcatgggtga ttctccgtat ttggttcaga
     3061 tagacttggt ttttgcgccg cagggcggtg gcttctttca agccgattat tttgaatttg
     3121 actttgctgc cgaagcgcac ctgtgccagc ctgcccaaat cggcggcggc aacggtagcg
     3181 attttcggat aaccgccggt ggtttgcgca tcggccagca ggataatcgg tttgccgccg
     3241 ggcggcacct gcacggttcc tgcctgaaca gcgtgggaca gcatttccaa aggttgcgac
     3301 agggtcagcg gctgtccgtc gaagcggtag cccatgcggt tgctatcgct ttgcagcgtc
     3361 cacgtttccc gttccagatt cagacgccct ttttcactga aagcggcata ttccgacgaa
     3421 ggaacaaggt ggacggtatc ggtaaacggt atcggggcaa tgccgacttt ggacaattcc
     3481 tgcgcacctt tgccgatggg gagacaatca cctttttgca gcattctgcc ctgatggccg
     3541 ccgaaaccgg ctttcaggtc ggtgcttctc gaacccatca cttccggcac atcaaatccg
     3601 cccgccacgc acacatagcc gtacatgccc tgcacggcac gcaccatttt caaggtctgc
     3661 cctttgcggg cggtataacg ccaatacgaa tagaccggtt cgccgtccaa ttccgcctga
     3721 tacacggcac cggtgagaca aaacggcgta tcccgttcaa acaccagcat tatcccgccc
     3781 aaagcgattt cgattgcggc cgtgccttcg tcgttgccca ataaaatatt acccgccgcc
     3841 aaagcaaccg tgtccatcgc accggcatga ccgatgccgt aacgccggtg tccgtagcgt
     3901 ccggtatcct gaatatgcgc cggtgcctgc actgccgaaa catgaatcat ggctcaatcc
     3961 tttctgcaac aaagcggact tggtcacccg ccgccagcag ggtcggcgga ttcaaatcgg
     4021 ctcggaacaa gggtaattcg gttctgccga taatctgcca gccgccgggc gaagcgaacg
     4081 gatacacacc ggtctgactg ccgccgatac cgaccgaacc ggcaggaacg gacgttctcg
     4141 gcacggcacg gcggggcgtg tgcaatgctt cgggcaagcc gcccagataa gggaaaccgg
     4201 gctggaagcc catcataaat acggtataag tttgcgccgt atggcggcgg acgatttcgg
     4261 aaataaccgt ctgatggaaa gcagcgactt ccgccaaatc cgggccgtat tcgccgccgt
     4321 agcagacggg aatttccacc agtttgccct gatggtctgt aacggcggtg tgttcccaca
     4381 catattgcaa ttcatcggca agcgtcgcca aatcggtatc gaaacgggtg aacacggtca
     4441 gattgttcat gccgaccacc acttcctcaa tcctgtcgtg ctgcccgagc gcagcggcaa
     4501 acgcccacaa cttttgctgt ttgcccagtt cggaaggcgc attcagtcgg cagaccaaag
     4561 cggattcgct gattggtgtg atctctattc tcatttgttg ttcattttgg ttatgtttta
     4621 atgaatctat atgcaggggc ggcggtttgt caatatcttc tgtgctgcat catcaaaccg
     4681 tcgattggaa aagtgctgcc ctgccgctgc actttttcag acgaccttaa accgtttcta
     4741 ttaaaatagc gcattccact tttcagacgg catccctatg tttcccgacc aatccgcccc
     4801 caacctcctg caaggcttga atcccgaaca actctccgcc gtaacctggc cgccgcaatc
     4861 cgcccttgtg ctggcgggcg cgggcagcgg caaaacgcgc gtgctgacca cgcgcatcgc
     4921 atggcttttg caaagcggac aagccagcgt gcatagcatt atggcggtaa cgtttaccaa
     4981 caaagccgcc aaagaaatgc agacccgttt gggcgcaatg attcccgtca acgtccgcgc
     5041 catgtggctc ggcacgtttc acggtctctg ccaccgcttt ttgcgcctgc accaccgcga
     5101 cgcaggcctg ccgtcttcct ttcaaatcct cgacagcggc gaccagcttt ccctcatcaa
     5161 acgcctgctc aaaagcctca acatcgccga agaaatcatc gcgccgcgtt cgctgcaagg
     5221 ctttatcaac gcgcaaaaag aatccggttt gcgcgcttcc gtcttgagcg cgcccgaccc
     5281 gcacacaagc cgcatgatcg aatgctacgc cgaatacgac aaaatctgcc aacgcgaagg
     5341 cgtggtcgat ttcgccgagc ttatgctccg cagctacgaa atgctgcaaa gcaacgaaat
     5401 cctgcgccag cattaccaaa accgcttcaa ccacattctc gtcgacgagt tccaagacac
     5461 caacaaactg caatacgcct ggctcaaact catggcgggc ggaaacgcag cggtatttgc
     5521 cgtcggcgac gacgaccaaa gcatttaccg tttccgtggc gcaaacgtcg gcaacatgac
     5581 cgcgctgatg gaagaattcc acatcgacgc gcccgtcaaa ctcgaacaaa actaccgctc
     5641 cgtcggcaac atccttgccg ccgccaatgc cgttatcgaa aacaacgacg aacgactcgg
     5701 caaaaacctg cgcaccgacg ccgaagcagg cgacaaaatt cgctactact ccgcctttac
     5761 cgacctcgaa gaagcccggt tcatcgtgga cgaaaccaaa gccctcgaac gcgaaggctg
     5821 ggatttggac gaaatcgccg tcctctaccg tagcaacgcc caatcccgcg ttatcgaaca
     5881 aagcctgttc cgcagcggca tcccctacaa aatctacggc ggcttgcgct tttacgaacg
     5941 ccaagaaatc aaacacgccc tcgcctacct gcgcctcgcc gtcaatcccg acgacgacaa
     6001 cgccctcttg cgtgtcatca acttcccacc gcgcggcatc ggtgcacgta ccgtcgaaaa
     6061 tcttcagacg gcatcgcaag agcaaggcgt ttcactttgg caggcagcct gcaatgccgg
     6121 cgcaaaagcc gccaaagtcg ccgccttcgt ccgcctcatt gaagccctgc gcaaccaagt
     6181 cggacaaatg cccctgcccg aaatcatcgc cggcatcctc aaagacagtg gcttgaccga
     6241 acactaccgc acccaaaaag gcgacaacca agaccgcctc gacaaccttg acgaactcgt
     6301 caacgccgcc atcgagttca aacccgaaga cagcaacttt gaaaccctgc ccgacaacat
     6361 ttcagacgac cccgccttcc ccatcctcgc tttcttgagc aacgccgccc tcgaatccgg
     6421 cgaaaaccaa gcaggcgcag gcgaaaaagc cgtccaactg atgaccgtcc acgccgccaa
     6481 aggcttggaa ttcaacgccg tcttcctcac cggcatggaa gaaggccgct tccccagcga
     6541 aatgagcctt gccgaacgcg gcggcctcga agaagaacgc cgcctcatgt acgtcgccat
     6601 cacccgcgcc cgcaaacgcc tctacatcac catggcgcaa caacgcatgc tgcacggaca
     6661 aacccaattc ggcatcgtct cccgcttcgt cgaagagatc ccacccgaag tattgcacta
     6721 cctgtccgtc aaaaagaccg cctacgacag ctacggcagc ccgcgccaaa ccgccgcatc
     6781 caaagacaaa atcatcgacg actacaaaca gccccaaacc tacgcaggtt tccgtatcgg
     6841 acaaaacgtc cgccacgcca aattcggcac cggcgtgatt atcgatgccg cagataaagg
     6901 cgaatccgcc cgactgacca tcaacttcgg caaacagggc gtgaaagagt tggataccaa
     6961 gtttgcgaaa ttggaagcga tgtaaatttg aaatgcaggc tggatactcg tatccgacaa
     7021 aaatatttga cgcgtctatc gtttccgaaa aaccgctgtt ggaaaatgtc ggattcgaga
     7081 atccgaccta tggcaaaaaa ctgtagcaag gacaaggcaa aaggccgtct gaatccggta
     7141 acaacaaatt ttgccgaaac ctccgccgtc attcccgcgc aggcgggaat ccagaccttt
     7201 cggcacggga acttatcgga taaaaggttt ctttagattc cacgttctag attcccgcct
     7261 gcgcgggaat gacggacggt aaaagccggg tatagatacc taacccttgt ttatagctga
     7321 aaatcaataa ggaagaacat tatgtcccaa gtttttaaag attttgattt gtcctccgta
     7381 tgggaatccg acagttgggc agatgaaaac tacaaagaag ccccgtttac ccctgaaatt
     7441 ttggctgccg tagaaagtga actgggctat aaattgccgc aaagttttat tgaattgatg
     7501 gcggtacaaa acggcggaat atttgtcaaa aactgttttc cgaccacaca gagaaattcg
     7561 tgggcgaaag atcatgtgca aatttgcgag gtatcgggca tcggctttga aaaagaaggg
     7621 agtttgtgcg gcgaaacagg gcaaaaactt tggatggaag aatgggaata tccgcccatc
     7681 ggcgtgtatt ttgccaacga cccgtcaggc ggtcatgcca tgtttgcctt ggactatcgg
     7741 gagtgtggaa aagacggcga gccgaaagtg gtgtttgtcg agcaagaatc ggattttgaa
     7801 atcgtcgaac ttgcccccga ttttgaaacc tttatccgca gcttgcggca tgaagatgag
     7861 tttattgacg aggaaatata aaacggcggt tgaaaaaccg gaatcatcaa gggaaaacgg
     7921 gcgaaataac ggataatcgc ttgaatccgt aaggaaaacg gtttggtgaa acgcgccatc
     7981 caagaccttt gcaaagaaaa actgtctccg acagcattga cattattaac agaacttatc
     8041 aagtttggag ctatctcaaa tataattcgg ttatcctttt gtatccatta aatcatatgc
     8101 ttcaattaat tgttgttcta gctcttgtac caattttgaa ttgcgaattc ctgacacaat
     8161 ctcaaattct tctgcatcta tgcaaacacc tgcataaatt tcaataacaa gggaacgcaa
     8221 taattgaagc tcttctcttg ttaaagaaat aataatgtca tcacctgtgt aattgattat
     8281 attcataata attttatttt tgtttgtcaa agtaagtttt gcctaaggtt ggtctaaatg
     8341 cagttccacc atcttttgaa tttgggtctc tgattacaat tgctccagac ttatcatccc
     8401 aaattgctct tatgtgtttg gattgtaatc ttcgaattcc caagaaaaaa atcgtaataa
     8461 gtttgaaagt gtcaaatccc aagtttcttt tgagcaatat tctaatattt tatcaatttc
     8521 acttttaata atcttatgat caaactgttc taatattaat gcattagacc aaaaaaaacc
     8581 ttctttatta caatgatggg aaatccattt aggagaacaa atgcaaagtg aaaaaataga
     8641 tgagccttgt tctccttcga ttccgatatc caaatctatc catctatgga aattatctgg
     8701 aatttcgggg gtaaattttt caaaatcaat atcatataaa tttatgcttt ttaaatccaa
     8761 tttaatcatt agggctgtcc tagataaata gggaaattca aattaagtta gaattatccc
     8821 tatgagaaaa agtcgtctaa gccggtataa acaaaataaa ctcattgagc tatttgtcgc
     8881 aggtgtaact gcaagaacag caacagagcc tgacagcatt gtttatacgg attgttatcg
     8941 tagctatgat gtattagatg tgagcgaatt tagccatttc cgtatcaatc acagcacaca
     9001 ttttgctgaa cgacaaaacc atatcaatgg aattgagaat ttttggaacc gggcaaaacg
     9061 tcatttacgc aagtttaacg gcattcccaa agcgcatttt gagctgtatt taaaggagtg
     9121 cgaatggcgt tttaacaaca gtgagataaa agttcaaatt tccattttaa aacaattagt
     9181 aaaatcgagt ttatcttagt tgtccaggac agccccatta tttttataac accgtgaagc
     9241 cgcacagcag tttgaacagt gatacgccgt ttgcgggctt acgagtttat tttcccggcc
     9301 tgcagtttga gcaatacggt gatttcctac ggttaataca aatgtttaca cattgataca
     9361 tttcatttat agttccgcct atttgaaaat agaaaatatg aattcgaccg caagtaaaac
     9421 cctgaaagga ttgtcgctgg tgtttttcgc ctctggattc tgcgccctga tttaccaggt
     9481 cagctggcag aggcttctat tcagtcacat aggtatcgat ttgagttcga ttactgtcat
     9541 tatttctgta tttatggtcg gcttgggtgt gggtgcgtat ttcggtggac gcattgctga
     9601 ccgttttcct tcaagtatca tccccctgtt ttgcatcgct gaagtatcca tcggtctgtt
     9661 cggtttggta agcaggggtc tgatttccgg cttggggcat cttttagttg aggctgattt
     9721 gcccatcatc gctgctgcca atttcctctt attgctgctt cctaccttta tgatgggcgc
     9781 gaccttgccc ttgctgacct gtttttttaa ccggaaaata cataatgttg gcgagtctat
     9841 cggtacctta tattttttca acactttggg tgcggcactc ggatcgcttg ccgccgccga
     9901 atttttctac gtctttttta ccctctccca aaccattgcg ctgacagcct gctttaacct
     9961 tctgattgct gcttcagtat ggctgcgtta cagaaaggat ggatatagtg aacactaaac
    10021 cgaatactag tttgatttat atgctttctt tccttagcgg cttattgagc ttgggtatag
    10081 aagtcttgtg ggtaaggatg ttttcgttcg cagcacagtc cgtgcctcag gcattttcat
    10141 ttactcttgc ctgttttctg accggtatcg ccgtcggcgc gtattttggc aaacggattt
    10201 gccgcagccg ctttgttgat attcccttta tcgggcagtg cttcttgtgg gcgggtattg
    10261 ccgacttttt gattttgggt gctgcgtggt tgttgacggg tttttccggc ttcgtccacc
    10321 acgccggtat cttcattacc ctgtctgccg tcgtcagagg gttgattttc ccgctcgtac
    10381 accatgtggg tacggatggc aacaaatccg gacgacaggt ttccaatgtt tatttcgcca
    10441 acgttgccgg cagtgcattg ggtccggtcc ttatcggctt tgtgatactt gatttcttgt
    10501 ccacccaaca gatttacctg ctcatctgtt tgatttctgc tgctgtccct ttgttttgta
    10561 cactgttcca aaaaagtctc cgactgaatg cagtgtcggt agcagtttcc ctaatgttcg
    10621 gcatcctcat gttcctactg ccggattctg tctttcaaaa tattgctgac cgtccggata
    10681 ggctgattga aaacaaacac ggcattgttg cggtttacca tagagatggt gataaggttg
    10741 tttatggggc gaatgtatac gacggcgcat acaataccga tgtattcaat agtgtcaacg
    10801 gcatcgaacg tgcctatctg ctaccctccc tgaagtctgg catacgccgc attttcgtcg
    10861 ttggattgag tacaggttcg tgggcgcgcg tcttgtctgc cattccggaa atgcagtcga
    10921 tgatcgttgc ggaaatcaat ccggcatacc gtagccttat cgcggacgag ccgcaaatcg
    10981 ccccgctttt gcaggacaaa cgtgttgaaa ttgtattgga tgacggtagg aaatggctgc
    11041 gtcgccatcc tgatgaaaaa ttcgacctga ttttgatgaa tacgacttgg tactggcgtg
    11101 cctattccac caacctgttg agtgcggaat ttttaaaaca ggtgcaaagc caccttaccc
    11161 cggatggtat tgtaatgttt aataccacgc acagcccgca tgcttttgct accgccgtac
    11221 acagtattcc ctatgcatac cgctatgggc atatggtagt cggctcggca accccggtag
    11281 ttttccctaa taaagaactg ctcaagcaac gtctctcccg gttgatttgg ccggaaagcg
    11341 gcaggcacgt atttgacagc agcaccgtgg atgctgcagc acaaaaggtt gtctctcgta
    11401 tgctgattca gatgacggaa ccttcggctg gggcggaagt tattaccgac gataatatga
    11461 ttgtagaata caaatacggc agagggattt aaccgtctta aagggtttca ggcaacgcag
    11521 gttttaggta acgtcctgct agttcaaaaa aaccgcatca cagcagtcgg gacaaaatgg
    11581 tttaaacatt ttgtcccgaa ttcttattcc
//
LOCUS       DAXHSF010000048        11458 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.048, whole genome shotgun sequence.
ACCESSION   DAXHSF010000048 DAXHSF010000000
VERSION     DAXHSF010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11458
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.048"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(35..2347)
                     /locus_tag="ACFM8S_000969"
     CDS             complement(35..2347)
                     /locus_tag="ACFM8S_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFC6314054.1"
                     /translation="MRHSHYSQWLSLPLLSVAVTQQLYAQPNESLPTVELEPVVITID
                     KSGMALANRITQMPHTTKVIYEEQIQEQATGSRQLADVMAQLIPSLGVSSGTTSNFGQ
                     TMHGRQVQFLLNGVPLTGSRDISRQLNSINPSQVARIEVLSGATSIYGSGATGGLINI
                     VTKSDLEEEQFETRIGVHGSKLSSEGIGYQVGQSVAGVSENGNVLARLDVDYRTTGGA
                     FDANGKRIAPEPAQTDKQDSKSLSVNTNVDWQLDDKQNINLALTHYNDKQDTDYAPDY
                     GNRLAVLFREKPSLNAIKGLSLSEQPKTTKSTFNINYHHDDLWGNTINTNAYYRREKG
                     RFYPFVAPFSTDKALPILQSMNLPSATLDALRARAYGVLQSESKADVLGARLALQRKF
                     DLAQKPVLLSYGIDYEQEKNRQGATGYDLTTFMQSNGLAFKDTGNRYTYGPDTTLKTY
                     GIFANANVALTDDLKISAGVRHQIIDSKTDGFVPPSESLLADLLKPYQAGTVNSGKVK
                     HHKTLFNLGANYSLTPNHSIFANFGQGYSLPDLQRVLRDVGTGFAVNSDNVEPISVNS
                     YDAGWVGVFGGSRAKLTGFYNTSDKVTQFLKDYSVVVADTDERIYGAEFELNHKINDN
                     WAVGGSIAYTRGQYKDASGQYRELGAFRISPTKATAFAQYTTPSEHKLRLQLLAIDGT
                     QRAYKDSLIAKTNQNVRKTPEARISGYVVADLLGEIKLPKGQLNFGIYNLTNKKYKSV
                     FSQAAKVTYGELSSLSAQGRSYGISYTLNY"
     gene            complement(2394..3644)
                     /locus_tag="ACFM8S_000970"
     CDS             complement(2394..3644)
                     /locus_tag="ACFM8S_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC6314055.1"
                     /translation="MIVSQQKNRHLLTMVALLYVAHALPLYFFNVALPAILRHQGVDI
                     RVIGMLSLLYLPWAFKFLWASYVDNYYCAKIGKRKTWLFFTQIIVVVGLFGLGFLQFS
                     EIVLFLSISFLVSLASATQDIAIDGYTVEQFDKSSFGLASAMQSFGVAFGSMVGGAGT
                     LWLYQRFDWQMATISLATTVVLISCSLWFLKDQDNPKHATNLSKASLIRLIKRPQTKW
                     LLALIVVFRFVEAPMMAMLNPMLVDFGFSLSQIGLLFSVFGAIVGIGSALLAGVLIKY
                     IDIVRCLILFGWLRTAVYALLCLGLLYPLLGDFLPAVGLLKTGFMSLMAISVLLILSV
                     RYLTMTALYALFMNAANPDQAGTDFTLFVCVELLVYFIGGAMSGFLVHQFGYDKLFLW
                     LAIGSGVSMLILPTLIKKFSVTDC"
     gene            complement(3644..4042)
                     /locus_tag="ACFM8S_000971"
     CDS             complement(3644..4042)
                     /locus_tag="ACFM8S_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314056.1"
                     /translation="MPIFLNAIQNPNSSLPVFLLDFSGEISQDAFDDWLIAMSDLLAS
                     EQSFALIYHSRTGLTLPDNYRQIEAVWYKTHKESFFKYCKGLVRIVDDETQRQALDSP
                     AFHKAWRVPYAVVLSLTEAKAWINTKIGAV"
     gene            complement(4047..4649)
                     /locus_tag="ACFM8S_000972"
     CDS             complement(4047..4649)
                     /locus_tag="ACFM8S_000972"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC6314057.1"
                     /translation="MPTLSQGLPNNYIYRENGVEFGLVQIRYPEHIELLHRWMNMPHV
                     IPQWQLNKSLSELSVYFEKMLADDHQRLYLVSINGEWVGYAEVYEGARDRLSGYYTAD
                     KNDLGWHLLLAEAQAVGKGYLRAIMRLLSFFIFEHSPAKKIVGEPDASVKPYEVVAQE
                     LNYMPQYQIVMPEKTAMLYFCDKEGFYCKFAHYLNASIKE"
     gene            complement(4834..5082)
                     /locus_tag="ACFM8S_000973"
     CDS             complement(4834..5082)
                     /locus_tag="ACFM8S_000973"
                     /inference="COORDINATES: protein motif:HMM:NF018031.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IucA/IucC family C-terminal-domain containing
                     protein"
                     /protein_id="HFC6314058.1"
                     /translation="MSEFEFWNELSKIIDEFHHQHPELNERIALFDLKRPKFEKVCLN
                     RVRFFTRGYQDNANRPEPVVCEPICNPISPQFLRCVEH"
     gene            complement(5262..5573)
                     /locus_tag="ACFM8S_000974"
     CDS             complement(5262..5573)
                     /locus_tag="ACFM8S_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HFC6314059.1"
                     /translation="MEYLTTTIPTHFEIERTRLADVILTKLSDDKAVKLAKQMLDEHE
                     YIESLLVNTDPSVDDVKELANALYDHIRFEERELFPIAETVLSDDELFAIYEASDENV
                     K"
     gene            complement(6102..6794)
                     /locus_tag="ACFM8S_000975"
     CDS             complement(6102..6794)
                     /locus_tag="ACFM8S_000975"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFC6314060.1"
                     /translation="MKLIKYLQYQGIGSRKQCQWLIRGGYVSINGTCMDDTDADIDSS
                     SVETLDLDGEAVTVVPEPYFYILLNKPEDYETSHKPKHYRSIFSLFPDNMRNIDMQAV
                     GRLDADTTGVLLITNDGKLNHNLTSPSRKIPKLYEVTLKHPTGETLCETLKNGVLLHD
                     ENETVCAADAVLENPTTLLLTITEGKYHQVKRMIAAAGNRVQHLHRRRFAHLETENLK
                     PGEWKFIDCPKL"
     gene            complement(6862..8838)
                     /locus_tag="ACFM8S_000976"
     CDS             complement(6862..8838)
                     /locus_tag="ACFM8S_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFC6314061.1"
                     /translation="MSKLKTIALTASGLSVCPGFLYAQNTSSHQIGLAIMRLNSSILD
                     LPPTKQYFQSGSLWSELRQGFRMGEVNPELVRRHESKFIASHSYFNRVINRSRPYMYH
                     IANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDG
                     RHDVYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAINRARAQGLEPTYENL
                     RMPNETRNYVPKLLAVRNIIATPQSFGMNISDIDNKPYFQAVEPDRPLDNEAIARLAG
                     ITQSELLALNPAFNVPAFIPKSKRKLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAA
                     KTSLSDISTATGMSIADIKRLNNLNGNLVNAGRSILVAKNGKTLHTASESVVSIDIDN
                     TPDTYRSNMPAGTVNVGIARIRPAAAQTADITVAPLPQKIVRTEPDPLVRIAEPALAT
                     AAAQPQTEKQTAMPSETQTATLAQIIPQNDMQAADELMQLVARNNLRRQAEETISAVI
                     GTPDTVAEHNISSSPQHTVAADGKRRARLETRVAKAADGEAEISPLHASIHRVVEGDT
                     LFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFK
                     SIAARFNIHIDDIRRLNPNLNTINPGQRVKLIGS"
     gene            9017..9682
                     /locus_tag="ACFM8S_000977"
     CDS             9017..9682
                     /locus_tag="ACFM8S_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFC6314062.1"
                     /translation="MDSWFEDTAMGRYVAKLEQDFFGRYLDSYRFSGMCAVQVGGPWL
                     SLSEDVVCVPRDMSMSAENMALADVSADMLLFPHTLEGGVPSQILSEAHRILKPSGRL
                     MLTGFNPYSLWGFSRWFDGERLPEKRFCLPLPELKRQLADVGFDIEFGKFMVYLPPVS
                     SLGQIRFWRFMEKAGDRWWPQCAAVYGLVLVKRAAGVTPLPEWDGYLGGKALAAGAAR
                     VAD"
     gene            9734..10561
                     /gene="mutM"
                     /locus_tag="ACFM8S_000978"
     CDS             9734..10561
                     /gene="mutM"
                     /locus_tag="ACFM8S_000978"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFC6314063.1"
                     /translation="MPELPEVETTLRGIAPHIEGKTVEAVVLRQLKLRWQINPDLGEI
                     LSGRQVLSCGRRAKYLLIRFQTGVLLIHLGMSGSLRIFTPSDGRIGRPDRHDHVDIVF
                     SDGTVMRYRDPRKFGAILWYEGIEEHHPLLEKLGPEPLSEAFCTDYLYVRLKAQKRAL
                     KLALMDNAVVVGVGNIYANESLFRAGISPHRPANRLKKKECALLVETVKAVLQRAIET
                     GGSTLRDFVDSDGKSGYFQQEYTVYGRHNQPCPQCGGLVVKETLGQRGTFYCPNCQK"
     gene            10649..11416
                     /locus_tag="ACFM8S_000979"
     CDS             10649..11416
                     /locus_tag="ACFM8S_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HFC6314064.1"
                     /translation="MSSNKASFFTRLRRLCRLAVWLFKTGKNLRGIDGGCPESRNRAV
                     IELGRGVLAALDIGLEVGRPAPEHPNGVLVAANHVSWLDIFAMSAVYPSSFIAKQEIK
                     SWPVLGKMGQNAGTVFINRNSRRDIEPINRAVCETLQRGQNVSFFPEARTSSGLGLLP
                     FKAALFQSAIDAGAKVLAVALRYYDETGKRTARPSYADVGLPTCLWRIVSMKKLTIRV
                     DFVCVADAAESEDRYALKDKIEESIRAVVADDADIAV"
BASE COUNT         2940 a         2453 c         2876 g         3189 t
ORIGIN      
        1 gcgatgtttg tggtattgtg gcacataatg ctgatcaata atttaaggta taacttatcc
       61 cataacttct cccctgtgca gacaagctgg ataattcacc ataagtcact ttggcagctt
      121 gactaaatac acttttatat tttttatttg tcaagttata aatcccaaag tttaactgac
      181 ctttaggtag tttgatctca cccaataaat ccgccacgac atagcctgaa attctagcct
      241 ccggggtttt gcggacattt tgattggttt tggcaatcag gctgtccttg taagctcttt
      301 gtgtaccatc aatggcaagt agttgtaagc gtaacttatg ttcacttggt gtggtgtatt
      361 gagcaaaagc ggtggctttg gtagggctga tacgaaacgc cccaagttct cgatattgac
      421 cgcttgcatc tttgtattga cctcgtgtat aggcaatgct accaccgaca gcccagttat
      481 cattaatctt gtgatttaat tcaaactccg ccccataaat acgctcatca gtatcagcaa
      541 ccaccacgct ataatctttt aaaaactggg ttactttatc tgatgtatta taaaaacctg
      601 tcaacttggc acggctaccg ccaaacactc ctacccaccc tgcatcataa ctattgactg
      661 aaattggctc aacattatca gaattaaccg caaaacccgt cccaacatct cttagcaccc
      721 tttgtaaatc aggcaaacta tatccttgcc caaaattggc aaaaatactg tgatttggag
      781 ttaatgagta atttgctcca agattaaata aggttttgtg atgcttcact ttgccagaat
      841 tgacagttcc tgcttgatac ggctttaata aatcggctaa tagcgactca ctgggtggca
      901 caaaaccatc tgttttactg tctataattt gatgccgtac acctgccgat atttttaaat
      961 catctgtcaa agcaacatta gcattggcaa atatgccgta ggtttttaag gtggtgtcag
     1021 gtccataagt gtaacggttg ccagtgtctt taaatgccaa gccgttgctt tgcataaagg
     1081 tagtcaaatc atagcctgtc gccccttggc ggtttttttc ttgttcataa tcgataccat
     1141 aactaagtag tacaggtttt tgagcaaggt caaattttct ttgcaaagcc agtcttgctc
     1201 ccaatacatc tgccttggat tcggattgta acaccccata ggcgcgagct cgtagagcat
     1261 ccaaagtggc tgatggcaaa ttcatgcttt gtaaaatagg cagggctttg tcggtcgaaa
     1321 acggggcaac aaagggataa aatctgcctt tctctctgcg ataataagca ttggtattga
     1381 tggtgttacc ccacaaatca tcatgatgat agttgatatt aaaggtgctt ttggtggttt
     1441 ttggctgttc tgataatgat aagcctttga tggcatttaa tgaaggcttt tctcgaaaca
     1501 acaccgcaag gcgattacca taatcaggtg cataatcggt atcttgtttg tcgttataat
     1561 gcgtcaatgc cagattgata ttttgcttgt cgtcaagttg ccaatcaaca tttgtattga
     1621 cacttaggct tttgctgtct tgcttatcag tttgggcagg ctcaggagcg atgcgtttac
     1681 cgttagcatc aaatgcccct cctgtggtgc gatagtcgac atcaagtcgt gcaaggacat
     1741 taccattttc gctgacacct gctacactct gacctacctg ataaccgata ccttcactgg
     1801 ataatttact accatgtaca ccgatgcggg tttcaaattg ctcctcttcc aaatccgact
     1861 tagtaacgat attaatcaaa ccgcctgttg ctccagaccc ataaatactg gttgctcctg
     1921 ataaaacttc aattctagcc acttgactgg ggttgatgct attaagctgt ctagagatgt
     1981 ctcgcgaacc cgtcaaaggc acaccattta acaaaaattg tacttgacga ccgtgcatgg
     2041 tttgcccaaa gttactggta gtgccactac ttacccccaa acttggaatg agctgtgcca
     2101 tcacatcggc aagctgtcga gagcctgttg cttgctcttg aatttgctct tcataaataa
     2161 ctttggtggt atggggcatt tgcgtgatac gattggcaag tgccataccg ctcttatcaa
     2221 tggtaataac cacaggctct aattcaaccg ttggtaatga ctcattgggt tgagcgtaca
     2281 actgctgagt tactgccaca cttagtaaag gcaaagataa ccattgagaa tagtgggaat
     2341 gacgcataaa ttactcctta tggaaaagta aaattttaac agtctgttac ggtttaacag
     2401 tctgttacgg aaaatttttt tatcaatgtc ggcaaaataa gcattgacac cccagaacca
     2461 atcgcaagcc acaaaaacag cttgtcatag ccaaactgat gtactaaaaa tccactcatc
     2521 gccccgccga taaaatacac aagcaactcc acgcacacaa acaaagtaaa gtccgtgcca
     2581 gcttggtcgg gattggcagc attcataaac agagcatata aggcagtcat tgttaaatag
     2641 cgaacagata aaattagcaa aacactaatt gccatcaagc tcataaaccc tgtttttaat
     2701 aaacctaccg caggtaaaaa atcacccaat aaaggatata gcaatcctaa acacaacaaa
     2761 gcataaactg ccgttcttag ccatccgaat aatataagac aacgtacaat gtcgatatat
     2821 ttgattaata cccctgccaa aagtgctgaa ccaatcccaa caatcgctcc aaataccgaa
     2881 aacaaaagcc caatctgaga cagtgaaaaa ccaaaatcca caagcattgg atttagcatt
     2941 gccatcatcg gagcttccac aaagcgaaac acaacaatca aagctagtaa ccatttagtt
     3001 tgcgggcgtt tgataagcct aataagactt gctttggaaa gattggtagc atgtttggga
     3061 ttgtcttgat cttttaagaa ccatagtgaa cacgaaataa gcacaacagt tgttgccaaa
     3121 ctgattgtcg ccatttgcca atcaaatctc tgatatagcc acagtgtgcc agcaccgcct
     3181 accatactac caaacgccac tccaaaactt tgcatggcac ttgccagccc aaaggatgac
     3241 ttatcaaatt gttccactgt atagccatca atagcgatat cttgggtggc acttgccaaa
     3301 gacaccaaaa aacttatgct cagaaataat acaatctcag aaaactgtaa aaagccaagc
     3361 ccaaacagcc cgactaccac gataatttga gtaaaaaata accaagtttt gcgtttgccg
     3421 attttggcac aataataatt gtccacataa cttgcccaca gaaatttaaa cgcccacggc
     3481 aaatataaaa gtgaaagcat gccaatcacc ctaatatcaa cgccttgatg tcttaaaatg
     3541 gcaggcaatg ccacattaaa aaaatacaaa ggcaaggcat gggcaacata taacagtgcc
     3601 accatcgtca aaagatggcg atttttttgt tgtgagacaa tcatcagacc gcccctattt
     3661 tagtgtttat ccatgctttg gcttcagtca gtgataacac caccgcataa ggcactcgcc
     3721 atgctttatg aaaagcagga ctgtctaagg cttgtctttg agtctcatca tcaacaattc
     3781 tgactaatcc cttgcaatat ttaaagaacg attctttgtg cgtcttgtac caaaccgcct
     3841 caatctggcg gtagttatca gggagtgtca gccctgtccg actatggtaa atcaaggcaa
     3901 aactctgctc actggctaac aaatcactca tagcaatcaa ccaatcatca aatgcatctt
     3961 gactaatctc gcctgaaaaa tcgagtaaaa atacaggtaa ggagctattt gggttctgaa
     4021 tagcatttag aaaaataggc ataacatcac tctttaatgc tggcatttaa ataatgggca
     4081 aacttacaat aaaacccttc tttgtcacaa aaatataaca ttgcagtttt ttcaggcatt
     4141 acaatttgat attggggcat atagtttaat tcttgagcga ctacctcata gggttttaca
     4201 cttgcatcag gttcacccac aattttttta gcaggtgaat gttcaaaaat aaaaaagctt
     4261 agtagacgca tgatggcacg caagtaccct ttgccaacag cctgtgcctc tgctagtaac
     4321 aaatgccaac ccaaatcatt tttgtcagcc gtataatacc ctgatagtct atcccttgcc
     4381 ccttcataaa cttcggcgta accaacccat tcaccattaa tactaaccaa atacaaacgc
     4441 tggtgatcat cagccaacat tttttcaaaa taaacagaca gctcagacaa tgatttattg
     4501 agttgccatt gaggaataac atggggcata ttcatccaac gatgcaacaa ttcaatatgt
     4561 tcagggtagc gtatttgcac caacccaaat tcaaccccat tttctctgta aatatagttg
     4621 tttggcaacc cctgagataa agttggcaat cgattgattt tactgcattg atcttgatta
     4681 aaagaatcgg tatagatttc tagcatttct attctctcat tagggtgtgt tgaatatttg
     4741 gtatttgcaa tgaaaaatga gaaaaatcgc acgtttttca tgcaaaaact aataaaagcg
     4801 ttaaaatttt catcattttt acaaataatg ttatcaatgt tcaacacacc ttagaaactg
     4861 tggtgatatt ggattgcaaa ttggttcgca aaccacgggt tctggacggt tggcgttatc
     4921 ttgataacct cttgtaaaaa agcgaacacg atttaggcac accttttcaa attttggtct
     4981 ttttaaatca aataaagcaa tcctctcatt tagctctgga tgttgatggt gaaattcatc
     5041 aataatcttt gataactcat tccaaaattc aaattcagat atattataat attaaagcta
     5101 ctttgtggcg gacagcgtgg agcatagcac cgataaacct gtgtttaatc agattgtggg
     5161 attaaaagat gggtttaaat aacttttagc aattttaaaa ttaaacgaaa gccaaacgtg
     5221 gtgtttggct ttttaattcc attgcccagc ttttaactcg ttcattttac attctcatca
     5281 ctcgcctcat aaatggcaaa aagctcatca tcgcttagca ccgtctcagc aatgggaaaa
     5341 agctctcgct cttcaaagcg gatatggtca tacagagcat ttgcaagctc cttgacatcg
     5401 tcgacgcttg ggtcggtatt gacaagtagg ctctcaatgt attcatgctc atcaagcatt
     5461 tgctttgcca atttaaccgc tttgtcatcg gataattttg tcaaaataac atcggcaagg
     5521 cgtgttcgct caatctcaaa atgcgtggga atggtagtgg tcagatactc cacttaggtt
     5581 aatcggctgg atcaggtttg acgattggtg ttggaagaca tattgcaaca gtgtttgatt
     5641 aagatgagat ggaaattcta cacgcgtttt ttaacaggaa ctgactgatt gttttttaag
     5701 tgtgtagtta ttttcactaa tagagttata ttttatagtg gatatttctg atttgcataa
     5761 atcaacaagg attgaatcag attttataga tagatgaatc gtttattttt ctacactggg
     5821 acggtgaaat aaaatttcat atgtcatatg atcgggaatg ctgcgtcagt aacatatgta
     5881 gtatgtgtag taaacgaaga taaaaaaacc atctatacaa ggggagaagt atagatggca
     5941 aaacacatta cggggaaaac gtcttactca taagcctgct tgaacaggcg ttactcagac
     6001 aaatggatta tatcgtaaat tgattttttg cgttaaattg ggttaaacca tatatttaat
     6061 gagtatgtgg atgaatataa atggaattct tggatgttat tttagagttt cggacaatcg
     6121 ataaatttcc attcgccggg tttcaggttt tctgtttcca aatgtgcgaa tcgccggcga
     6181 tgaaggtgtt gcacgcggtt gccggcggcg gcgatcatgc gtttgacttg gtggtatttt
     6241 ccttcggtaa tggtcagcag cagggtggtc gggttttcca aaacggcatc ggcggcacaa
     6301 acggtttcgt tttcgtcgtg gagcagcacg ccgtttttca aggtttcgca gagcgtttct
     6361 cctgtggggt gtttgagcgt tacttcgtat agcttgggaa tttttctgct cggcgaagtc
     6421 aggttgtggt tcagtttgcc gtcgttggta atcagcaata cgccggtcgt atctgcatcc
     6481 agcctgccaa ctgcctgcat gtcaatattg cgcatattgt cggggaacag gctgaatatg
     6541 ctgcggtagt gtttcggttt gtgcgaagtt tcgtaatctt caggcttgtt gagcaggatg
     6601 tagaaatagg gttcgggaac gacggttact gcttccccgt caagatccaa cgtttcgacg
     6661 gatgaggaat cgatgtctgc atcggtgtcg tccatacagg ttccgttgat ggagacgtag
     6721 ccgcccctaa tcagccattg gcactgcttg cggcttccta tgccttgata ttgcaggtat
     6781 ttgataagtt tcatgatggt atgggaaatg tgggatgaaa aagacaggac tgagaaagtc
     6841 ctgtcatgtg ccgtatccga atcagcttcc aatcagtttg accctctgtc ctggattgat
     6901 ggtgttcagg ttgggattga gccggcggat gtcgtcgata tggatattga agcgcgcggc
     6961 gatacttttg aaggtgtcgc ctttgcgcgc ggtgtaggat actttttcaa tacgggtttg
     7021 ggcaggggct gcctgcgcca ggcggagtac ctgtcctttt tggatggtgt tgcctttgat
     7081 gttgttggcg acaatcaggt cggctacgct gacgttgtag cgtttggcaa tgttgaacag
     7141 ggtgtcgcct tctacaacgc ggtggatgct ggcatggagc ggggagattt cggcttcgcc
     7201 gtcggcagct ttggctacgc gcgtttccaa acgtgcccgg cgtttgccgt cggcggcaac
     7261 ggtatgttgc ggagatgagg aaatgttgtg ttcggcaact gtgtcaggcg tgccgatgac
     7321 ggcggagatg gtttcttcag cctgtcggcg caggttgttt cgggcaacaa gctgcatgag
     7381 ttcgtctgcc gcctgcatgt cgttttgggg gatgatctgc gcgagtgttg cggtttgggt
     7441 ttcagacggc atggcggtct gtttttcggt ttgaggttgc gctgcggctg tcgcaagggc
     7501 aggttcggca atacggacaa ggggatcggg ttccgtacgg acgattttct gcggcaaagg
     7561 tgcgacggta atgtccgctg tctgtgcggc ggcgggtcgg attcgggcaa tgccgacgtt
     7621 caccgtgcct gccggcatat tggaacggta ggtgtcgggc gtattgtcga tgtcgatgga
     7681 aacgacggat tccgatgccg tatgaagggt tttgccgttc ttggcgacaa ggatgctgcg
     7741 tcctgcgttg acaaggttgc cgttcaggtt gttgaggcgt ttgatgtcgg caatgctcat
     7801 gccggttgcc gtcgagatgt cggacaggct ggttttggcg gcaggcgtat agacttccca
     7861 tgaaaacagg ctgtcgggtg cggcgttgag gtagttgctt tggaaggttt gtacggacgc
     7921 gacaggaagc agcagtttgc gtttgctttt ggggataaac gcggggacgt tgaatgcggg
     7981 gtttagggcg agcagctcgc tttgcgtgat gccggcaagc cgggcgatgg cttcgttgtc
     8041 gagcggacga tccggttcga ctgcctgaaa atagggtttg ttgtctatgt cgctgatatt
     8101 catgccgaaa gattggggag tggcaataat gttgcgcacg gcgagcagct tggggacata
     8161 gttgcgcgtt tcgttgggca tacgcaggtt ttcgtaggtc ggttcgagcc cttgggcgcg
     8221 ggcgcggttg atggcgcgtc cgacgttgcc ttcaccccag ttgtaggcgg caaaggcaag
     8281 cggccagtcg ccgaacagtc catagaggta ttgcagatag ttgagtgcgg catcggtggc
     8341 ggcgtaaacg tcgtgcctgc cgtcgtaaac cggtgttttt tccaggccgt aatgcctgcc
     8401 ggtagcgggc atgaactgcc acaggcccga tgcgccgacg tgtgatttgg ctttggtgac
     8461 gaacgcgctt tcgatgaagg gaagcagggc ggcttcggcg ggcatattgc gttttttgac
     8521 ttcgttggcg atatggtaca tatagggtct actccggttg atgaccctgt tgaaatagct
     8581 gtggcttgcg atgaatttgc tttcgtggcg gcgtaccagt tcgggattga cttcgcccat
     8641 ccggaagcct tggcgcagct cgctccacag gctgccggat tggaaatatt gttttgtcgg
     8701 gggcaggtcg agtattgaag agtttaagcg cataatcgcc aaaccgattt ggtgtgatga
     8761 ggtgttttgg gcgtatagga aacccggaca aacggacaga cctgatgcgg tcagagcgat
     8821 ggttttgagt ttggacatga tttatattcg gcggaataaa catattttat cgtccgatgg
     8881 tactgtttga ctgaaaaagc gtcaagtttt acaggaactt tatgcggatt cggaaatggg
     8941 cggccgatgt gttttttgac agaaatcctg tttggaatag aatatcggca ggcaatctgt
     9001 ttgaaacgga cgcaatatgg attcatggtt tgaagatacg gcgatggggc ggtatgttgc
     9061 aaaattggaa caggatttct tcgggcggta tctggattca taccgttttt cgggaatgtg
     9121 tgcggttcag gtgggcggtc cgtggctgag cctgtctgaa gatgttgtct gtgtgccgcg
     9181 agatatgtcg atgtcggcgg aaaatatggc tttggcggat gtttctgcgg atatgctgct
     9241 tttcccgcat actctggaag gcggtgttcc ttcgcaaatc ctgtcggaag cgcaccggat
     9301 attgaaaccg tccggacgtt tgatgctgac gggtttcaat ccgtattcgc tctggggatt
     9361 cagccgttgg tttgacggcg aacgcctgcc ggaaaaacgg ttttgtctgc cgctgcccga
     9421 gttgaaaagg cagcttgcgg atgtcggttt cgatattgaa tttgggaaat ttatggtgta
     9481 tctgccgccc gtttcgtcgc tcgggcaaat acgcttttgg cggtttatgg aaaaggcggg
     9541 cgaccgttgg tggccgcagt gtgccgcagt gtacggtttg gttttggtca aaagggcggc
     9601 gggcgtaacg cccctgcccg agtgggacgg gtatttgggc ggcaaagccc ttgcggcggg
     9661 tgcggcaagg gttgcggatt agggacagcc gtctttcaga cggcatctgt cagaagaaat
     9721 gtcgggaggg gttatgcctg aattgccgga agtggaaacg acgttgcgcg gcatcgcgcc
     9781 gcatattgaa gggaaaacgg tggaagccgt ggtattgcgc caattgaagc tgcgctggca
     9841 gattaatccc gatttggggg agattttgtc cggccggcag gtgttgtcct gcggcaggag
     9901 ggcgaaatac ctgcttatcc gctttcaaac gggcgtgctg ctgattcact tggggatgtc
     9961 gggcagcttg cggattttta cgccgtcgga cggacgtatc ggcaggccgg acagacacga
    10021 tcacgtcgat attgtgtttt cagacggcac ggtcatgcgt taccgcgatc cgagaaagtt
    10081 cggcgcgata ctttggtatg agggaatcga agaacatcat ccgctgttgg aaaaactggg
    10141 gccggagcct ttgtcggagg cattttgcac ggattatctg tatgtgaggc tgaaggcgca
    10201 gaaacgcgcg ctcaaacttg ccctgatgga caatgcggtc gtggtcggtg tgggcaacat
    10261 ttatgccaac gagagcctgt tcagagcggg catttcgccc caccgtcctg ccaaccgcct
    10321 gaaaaagaaa gagtgtgcgc ttttggttga aaccgtcaaa gcggtgttgc agcgcgccat
    10381 tgaaacgggc ggcagtacct tgagggattt tgtggacagc gacggcaaaa gcggctactt
    10441 ccaacaggaa tatacagtgt acgggcggca caatcagccg tgcccccagt gcggcggttt
    10501 ggttgtgaaa gaaactttgg ggcagcgcgg cacgttttat tgcccgaact gtcagaaata
    10561 ggactgaaaa cggtttcaga cggcatttta tcggtatgcc gtccgaacgt ttcaacaaca
    10621 aacaccgatt atcgggaaag aattgctcat gtcttcaaat aaagcttcat tttttacacg
    10681 tctgcgccgc ttgtgccgtt tggcggtctg gctgttcaaa accgggaaaa acctgcgcgg
    10741 tattgacggc ggttgccccg agtcgcgcaa tcgggcggta atcgagttgg gcaggggggt
    10801 tttggcggct ttggatatcg gattggaggt gggcagaccc gcacccgaac atccgaacgg
    10861 tgtcttggtt gccgccaacc acgtgtcctg gctggatatt ttcgcgatga gcgcggttta
    10921 tccgagcagc tttatcgcca agcaggaaat caaaagctgg ccggtattgg gcaagatggg
    10981 gcagaacgcg ggaacggtgt tcatcaaccg caattcgcgg cgcgacatcg aaccgattaa
    11041 ccgcgccgtc tgcgaaacct tgcaacgcgg tcaaaacgtc agttttttcc ccgaagcgcg
    11101 gacttcctcc ggattggggc ttttgccgtt caaagccgcg ctgttccaat ccgccatcga
    11161 tgcgggggca aaggttttgg cggtcgcgct gcgttattat gacgaaacgg gaaaaaggac
    11221 agctcgtccc tcatatgccg atgtcggttt gccgacctgc ctgtggcgca tcgtgtctat
    11281 gaaaaaattg acgataagag tcgatttcgt ttgcgtggcg gatgcggcgg aaagcgaaga
    11341 ccgttatgct ttaaaagata aaatcgaaga aagcatccgt gccgttgtcg ccgacgatgc
    11401 ggatatcgct gtctgaaacc ggttgtcgga atgtggcagt atgattcgct tttgtgga
//
LOCUS       DAXHSF010000049        11350 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.049, whole genome shotgun sequence.
ACCESSION   DAXHSF010000049 DAXHSF010000000
VERSION     DAXHSF010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11350
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.049"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            141..1538
                     /gene="aspA"
                     /locus_tag="ACFM8S_000980"
     CDS             141..1538
                     /gene="aspA"
                     /locus_tag="ACFM8S_000980"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFC6314065.1"
                     /translation="MTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQ
                     FVRSMVMVKKATAQANGELGAVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGT
                     SVNMNTNEVISNLALEALGYEKGRYDIVNPMDHVNASQSTNDAYPTGFRLAVYYSIGE
                     LLDKLVVLKNAFAAKAEEFKDVLKMGRTQLQDAVPMTTGQEFQSFQVLLEEEILNLDR
                     TRQLLLEVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYV
                     MVHGALKRTAVKLSKICNDLRLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVIPEVV
                     NQVCFKVIGNDTTITFAAEAGQLQLNVMEPVIAQCMFETISLLGNAAVNLSDKCVKGI
                     TVNREICERYVFNSIGLVTYLNPYIGHHNGDLVGKICAQTGKGVREVVLERGLLSEEE
                     LNRILSPENLMNPHL"
     gene            complement(1748..2623)
                     /locus_tag="ACFM8S_000981"
     CDS             complement(1748..2623)
                     /locus_tag="ACFM8S_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HFC6314066.1"
                     /translation="MAYLLISIVFSVSVSILLKMARKKKIDIAQAVAVNYVVAVILTL
                     LVLKPDVGNIGAFLPTWWLFAALGVLLPSVFVIMGKSVESAGIVKSDAAQRLSLFLPI
                     VAALTLFGEKLSEGRLIGLCLAFAALFCLLWKHSGGKKSGSAWRQAALLLGVWAGYGI
                     IDILFKQIAKSGTAFAGNLLVAFVLAGVLMFACLFAKSVRWRAESVVGGILLGGLNFM
                     NIVTYITAHQMMKDNPTLVFAGMNIGVIVLGTLSGALFFKEKINTINTAGIVLALCSI
                     ACLFYWAEVKALFGI"
     gene            complement(2625..3347)
                     /locus_tag="ACFM8S_000982"
     CDS             complement(2625..3347)
                     /locus_tag="ACFM8S_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HFC6314067.1"
                     /translation="MDKDLYAVLGVSPQAGADEIKRAYRKLAMKYHPDRNPGNPKAEE
                     KFKEIQRAYDTLSDLSKRMQYDASFRRHEERGRQEEAFRREQAHREQFYREQMRREQA
                     FRQAFERQASRSCHTYEPSGGGSGRNYVLAAYILFGLGAIMLFMPIVGVIFAYMPIVG
                     VILAYMKRNSLDSIVYAAHTEYLIKTFWRTFWLYILGALTALLGIGVLIIIATNVWYF
                     YRIIAGFIRFNGGRAVAPEKWI"
     gene            complement(3370..3849)
                     /gene="ybaK"
                     /locus_tag="ACFM8S_000983"
     CDS             complement(3370..3849)
                     /gene="ybaK"
                     /locus_tag="ACFM8S_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFC6314068.1"
                     /translation="MSKHTYPITPAVRVLRENGIKFEPFIYPYEEHGGTAQFARLFGK
                     DEHLVIKTIVLQDENGKGLIVLMHGDKQISTRNLARHLGAKRIEPATPAQANKWTGYL
                     VGGTTPFGIRTKLDIYVEQSVMDLETIYINGGKRGFIIGIRPGDLNILNPKTIQAAV"
     gene            3924..4286
                     /locus_tag="ACFM8S_000984"
     CDS             3924..4286
                     /locus_tag="ACFM8S_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFC6314069.1"
                     /translation="MDIRYFGTTPRYSEAVGANGLIFLSGMVPENGETAAEQTADVLA
                     QIDRWLAECGSDKAHVLDAVIYLRDMGDYAEMNGVWDAWVAAGRTPARACVEARLARP
                     EWRVEIKITAVKRDAATA"
     gene            4321..5184
                     /locus_tag="ACFM8S_000985"
     CDS             4321..5184
                     /locus_tag="ACFM8S_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HFC6314070.1"
                     /translation="MKTLLPFFGVVRVSGEDRQTFLHGQLSNDINHLQTGQACYATYN
                     TPKGRVIANMIVVNRGGDLLLIMAQDLLEATVKRLRMFVLRAKAVFEILEDYAVGAEL
                     EASAEPLAAQEPNLAFATQQDSDGICSIALPHGGILRIAPETALPPYDAAAESAWRLH
                     EIRSGYPWICAATKETAVAQMLNQHIIGGVHFKKGCYPGQEIIARAQYRGQVKRGLAV
                     LSGNSAVEAGTLLTADGEEAGIVLDSVQDSENFTALTVIKFSAAQKELAAPNGGIFKA
                     VHLFFKTENAE"
     gene            5278..6237
                     /gene="ttcA"
                     /locus_tag="ACFM8S_000986"
     CDS             5278..6237
                     /gene="ttcA"
                     /locus_tag="ACFM8S_000986"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFC6314071.1"
                     /translation="MSKKTKQELENNKLSKRLRHAVGDAINDFNMIEPDDKIMVCLSG
                     GKDSYALLDILRQLQASAPIDFQLVAVNLDQKQPGFPEEVLPTYLESIGVPYKIVEED
                     TYSTVKRVLDEGKTTCSLCSRLRRGILYRTAKELGCTKIALGHHRDDILATLFLNMFY
                     GGKLKAMPPKLVSDNGEHIVIRPLAYVKEKDLIKYAELKQFPIIPCNLCGSQPNLQRQ
                     VIGDMLRDWDKRFPGRIESMFSALQNVVPSHLADTELFDFVGLERGQTLKHGGDLAFD
                     SEKMPERFSDGSEEDESEIKIEPQKAERKVINILANKPKTCGA"
     gene            complement(6331..9270)
                     /locus_tag="ACFM8S_000987"
     CDS             complement(6331..9270)
                     /locus_tag="ACFM8S_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HFC6314072.1"
                     /translation="MSGFNYEKNQPHQMRAVSAVLGVFDGATPKYRTADENPELLFAA
                     KQYANNILKVQSQNGIDGRFPDRSDDQNILDISMETGTGKTYTYTQTMFELHRWLGVF
                     KFIVVVPTLSIKAGTRQFLQSKALAEHFEQDFGGDYEGVRLKTYVVESAKKNKGKKSN
                     APITIEQFVKAENKKEIHVLLINAGMVNSSSMTDTGDKALKDLFDNPADALAAVRPFV
                     IVDEPHKFPTRESAKTWGNIKRLKPQYILRYGATFNDEYYNLLYRLTAVGAFNDGLVK
                     GVRVFQEEMQGGMDAAVKLVSSDGKEAKFELNEKDKKQTFKLAKGEDLAKIHPAVSDL
                     KIDKMNKTVVVLSNGLELKTGAVINPYSYSQTVQDAMMQRAVAEHFKLERALLAERAP
                     QPKIKPLTLFFIDDIAGYRSGNELSGSLKDKFESWIHAEAARRLKTESDPFYRDYLQK
                     TLDDVSACHGGYFSKDNTDSDDRIEQEINEILHDKEKLLSLDNPRRFIFSKWTLREGW
                     DNPNVFQICKLRSSGSTTSKLQEVGRGLRLPVNELMARVRDVPYKLNYFVDSSEKDFV
                     KQLVGEINDNSFQEEISKKFTEELKQKILQKYPDIKPLGLLNKLFSDGIIDDNENFTE
                     DGYDKLKAAYPEAFPKGLDKGKVSNAKDEGKDTIIMREGKYEELKALWELIHHKAVLQ
                     YKIKDEAEFVDLFTAYLRENAAKFPQAGIRTAVNEAYINNGLMLSRRIDSFEDEDFIR
                     FNTMTYRELLEKLAQTAKIRMQTLHQAFYRVRNELNIGDFLNMQTIAQIKNGFNRFLL
                     HHSFHKFELDYQLVGSKIHPTKFTDKDGKPRAVKKADLGRFEDTEHRPVAGYLFDEIF
                     YDSDIEHENVANNQIEGVTVFTKIPRNSIKIPVAGGGTYSPDFAYIVKTKSGEILNFV
                     IEAKGTDGAEDLRKSEERKIKHAEKLFAEISKEIKVVFKTQFDGERIAELIGQNMPAG
                     GHSENGH"
     gene            complement(9361..>11350)
                     /locus_tag="ACFM8S_000988"
     CDS             complement(9361..>11350)
                     /locus_tag="ACFM8S_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC6314073.1"
                     /translation="DLSDTAGANTEAIYTSDGITANSTQLEQLKKLFPACFDADGNFL
                     IDRLQAEIAPQTDIGREFYEMNWLGKSYARLLRNLPPETLISEDKTHNAKPENAGSQN
                     LLIRGDNLEVLKHLKNAYTNSVKMIYIDPPYNTGSDGFVYQDDRKFTPAELALLANID
                     EDEAARILDFTDKGSNSHSAWLTFMYPRLYIARELLKDDGVIFISIDDNEAAQLKLLC
                     DEVFGEGNFVAQLPWRKRTAKSDVPFGISQDYEWIFVFAKSCQFIAATKGKERRYYET
                     DDFPDRPWRTHDLTKQTTAAERPNSFFTMVDPKTGKKYPANPNATWRVTKDTFQDYYN
                     KGKIVFPDDYDFLNISNPVMRYFKDDDMKKAGEDFGKVAVSSRLPENVGTLADAVAEY
                     LAIFSRTLPENIGMTKEGTKEITDLFGSKIFTFPKPSQLIKFLVSISSKSNDLILDFF
                     AGSGTTAHAVMQLNAEGQNGNRRYICVQLPEKTAEKSEARKAGYPTIFDITKARIEKA
                     AAKIRVEHPDYTGDSGFKIFQTADNFRQHPDKDFSPEQPDLPLNDELSEEQLQTLLTT
                     WTLYDGAALTTPVEPVRLGDYTAYLCEKRLYLMNAGFTSADLLAFIRKLDDDADFNPN
                     RVIVFGSNMASAMQHELDQAVRGYTNKKEIELNVVIRV"
BASE COUNT         2725 a         3050 c         2694 g         2881 t
ORIGIN      
        1 ccgaaaacaa cgtttcccgt tttcagacgg catacgatat ggacaatttc atccggccgg
       61 tatagtgttc gtcctgcggc ggcatctttc cgatggcgca ttttttcaat attaattatc
      121 cgacttacaa aggtaaaaac atgactgtcc gtatcgaaca cgatttattg ggcgaccgcg
      181 agattcccgc cgaagtgtat tggggcatcc acaccctgcg cgccattgaa aactttaaaa
      241 tctccacaca aaaaatttct gacgtgccgc agtttgtccg cagtatggtg atggtgaaaa
      301 aagcaaccgc gcaggcaaac ggcgaattgg gtgcagtaaa acccgaaatc gccgccgcca
      361 ttgaaaaggc ttgcgacgaa gttctgctga acaaccgctg cctcgaccaa ttcccctccg
      421 acgtgtatca gggcggtgcg ggtacttcgg tcaatatgaa caccaacgaa gtcatctcca
      481 acctcgcatt ggaagccttg ggatacgaaa aaggccgcta cgacatcgtc aatccgatgg
      541 atcacgtcaa cgccagccaa tccaccaacg atgcctaccc taccggcttc cgccttgccg
      601 tgtattacag tatcggcgaa ttgctcgaca aactggtcgt tctgaaaaac gcctttgccg
      661 ccaaagccga agaatttaaa gacgttttga aaatgggccg cacccagctt caagatgccg
      721 tgccaatgac tacaggacaa gaattccaat ctttccaagt gttgttggaa gaagaaatcc
      781 tcaacctcga ccgcacgcgc caactgcttt tagaagtcaa tttgggcgca acggcaatcg
      841 gcacgggcgt gaacacgccc aaaggctacg ccgaactggt ggtcaaaaaa ctctccgaag
      901 tcagcggctt gccttgcaaa ctgaccgaaa acctgattga ggcgacctcc gactgcggcg
      961 catatgtgat ggtacacggc gcattgaagc gcacggcggt caaactctcc aaaatctgca
     1021 acgacttgcg cctgctctct tccggcccgc gtgccggctt gaaagaaatc aacctgcccg
     1081 aattacaggc cggttcttcc attatgcctg ccaaagtcaa tcccgtgatt cccgaagtgg
     1141 tcaaccaagt ctgcttcaaa gtcatcggca acgacacgac gattaccttc gccgccgaag
     1201 ccgggcaact gcaattaaac gtgatggagc cggtcatcgc ccaatgtatg tttgaaacga
     1261 tttccctctt gggcaacgcc gcagtcaacc tgtccgacaa atgcgtcaaa ggcattacgg
     1321 tcaaccgcga aatctgcgaa cgttacgttt tcaactccat cggtttggtg acttatctga
     1381 atccgtacat tggccaccac aacggcgact tggtcggcaa aatctgcgcc caaaccggca
     1441 aaggcgtgcg cgaggttgtt ttggaacgcg gactgttgag cgaagaagaa ctcaaccgca
     1501 tcctctctcc tgaaaacctg atgaaccccc atctgtaacg ttgcagacaa atgccgtctg
     1561 aagcagcatc cctgcttcag acggcatttt cggcattcaa ggctgtttta gccgccgccc
     1621 gattccggcg gtttgtcgga aaaagccggt tatttccgtt cgggcaaaaa tctatcttat
     1681 taataaaaaa caattattta aaaccattcc ttaaagcgcg cccgcgcggt atgcggacac
     1741 ggcgggatta tatgccgaac agtgccttga cctccgccca ataaaacagg caggcgatgg
     1801 aacacagtgc caatacgatt cccgccgtat tgattgtatt gattttttcc ttaaaaaaca
     1861 gtgcgcccga aagcgtaccc aaaacaatca caccgatatt cataccggca aaaaccaagg
     1921 ttggattgtc cttcatcatt tggtgcgcgg tgatgtaggt tacgatattc ataaaattca
     1981 aaccgcccaa gagtatgccg ccgaccacac tctctgcgcg ccatctgacc gatttggcaa
     2041 acaggcaggc aaacatcagc acacccgcca gcacaaatgc aaccagcagg ttgcccgcaa
     2101 atgccgtgcc gcttttggca atctgtttga acaggatatc gataatgccg taacccgccc
     2161 acacgcccag cagcaatgcc gcctgccgcc acgcgcttcc tgattttttg ccaccgctgt
     2221 gtttccaaag caggcagaac agtgcggcaa atgcgaggca cagcccgatt agtctgcctt
     2281 cgctgagttt ttcgccaaac agcgtcaagg cggcaacaat cggcaaaaac agcgacaaac
     2341 gctgcgccgc gtcggatttg acgataccgg cggattctac ggatttgccc ataatgacaa
     2401 atacggacgg cagcagcacg cccaatgcgg caaacagcca ccatgtcggc aaaaatgcgc
     2461 cgatattgcc gacatccggc ttcaatacca gcagggtcag tatgaccgcg accacataat
     2521 tgacggcgac cgcctgcgcg atgtcgattt ttttcttcct tgccattttc agcaaaatgg
     2581 aaaccgacac gctgaacacg atgctgatta acaggtaagc catactatat ccatttctcg
     2641 ggtgcaaccg ccctgccgcc gttgaagcgg ataaagccgg cgatgatgcg gtagaaatac
     2701 cagacgttcg ttgcaataat aatcagcacg ccgataccca aaagggcagt cagcgcaccc
     2761 aaaatataaa gccaaaatgt gcgccaaaag gttttaatca ggtattcggt atgtgcggca
     2821 tagacaatgc tgtccaaact gttccgtttc atataggcga gaatcacgcc gactatgggc
     2881 atataggcga aaatcacgcc gactatgggc atgaacagca tgattgcacc caaaccgaac
     2941 aggatgtagg cggcaaggac atagttgcgc ccgcttccgc cgccggacgg ttcgtaagta
     3001 tggcacgaac gtgatgcctg ccgttcaaac gcctgtctga acgcctgttc gcggcgcatc
     3061 tgttcgcggt aaaactgctc cctgtgcgcc tgttcgcggc ggaatgcctc ttcctgccgc
     3121 ccgcgttcct catgccgtct gaaggacgcg tcgtattgca tccgtttcga caggtcggaa
     3181 agcgtatcgt aagcccgttg gatttctttg aacttttctt ccgccttcgg attgcccgga
     3241 ttgcggtcgg gatgatattt catcgccagc ttgcggtagg cgcgtttgat ttcgtccgct
     3301 cccgcctgcg gcgacacgcc caatacggca tacaaatctt tgtccatatt gttcctttat
     3361 actttcccgt caaaccgccg cctgtattgt tttcgggttc aaaatattta aatctccggg
     3421 acggatgccg ataatgaacc cgcgttttcc gccgttgata tagatggttt ccaaatccat
     3481 caccgactgt tcgacgtaaa tatccaattt tgtccggatg ccgaacggcg ttgtgccgcc
     3541 gaccaaatag cccgtccact tgtttgcctg tgcgggcgta gcgggttcga tgcgtttcgc
     3601 acccaaatgc cgagccagat tgcgcgtgga aatctgtttg tcgccgtgca tcaagacaat
     3661 cagcccctta ccgttttcat cttgcaaaac aatggtttta atgaccaagt gttcgtcttt
     3721 gccgaatagt cgggcaaact gcgccgtgcc gccgtgttcc tcgtagggat agatgaaagg
     3781 ttcgaacttg atgccgtttt cacgcaaaac gcgtacggca ggtgttatcg gataagtgtg
     3841 tttactcatt tttcgtcatg caaaacatgg aaatcggtca agcgcaatgt taccatatat
     3901 cccctttatc gaaggaaacc gatatggata tccggtattt cggcacaacg ccccgctatt
     3961 ccgaagccgt cggcgcaaac ggcttgattt tcctctccgg catggttccc gaaaacggtg
     4021 aaacagctgc cgaacagact gccgacgtgc ttgcccaaat cgaccgctgg ctggcggaat
     4081 gcggctcgga caaagcgcac gttttggatg cggtcatcta tctgcgcgat atgggcgact
     4141 acgcggaaat gaacggcgtg tgggacgctt gggttgccgc cggcagaacg cccgcccgcg
     4201 cctgcgtgga agcgcggctc gcccgtccgg agtggcgcgt cgaaatcaaa ataaccgccg
     4261 tgaagagaga cgcggcaact gcataaacag atttccttta tatttcagag tacgcaaatg
     4321 atgaaaacct tactgccctt tttcggcgtg gtgcgcgtca gcggcgaaga ccgccaaacc
     4381 ttcctgcacg gacagctttc caacgacatc aaccaccttc agaccgggca ggcgtgttat
     4441 gcgacttaca acacgcccaa aggccgcgtc atcgccaata tgattgtcgt caaccgaggc
     4501 ggcgatttac tgctgattat ggcgcaagac ctgcttgaag caaccgtcaa acgcctgcgg
     4561 atgtttgtcc tgcgtgccaa agccgttttt gaaatccttg aagattacgc cgtcggcgcg
     4621 gaattggaag catccgccga accgcttgcc gcgcaagaac ccaaccttgc ctttgccaca
     4681 caacaagatt cagacggcat ctgctctatc gctctgcctc atggcggcat ccttcgcatc
     4741 gcacccgaaa ccgccctgcc cccttatgat gccgctgccg aaagcgcgtg gcggctgcac
     4801 gaaatccgca gcggttatcc gtggatatgc gcggctacca aagaaaccgc cgtcgcccaa
     4861 atgctcaacc agcacatcat cggcggcgta cacttcaaaa aaggctgcta tccgggacag
     4921 gaaatcattg cccgtgccca gtatcggggg caggtcaaac gcggcttggc ggtattgtcg
     4981 ggaaattcgg ccgtcgaagc aggaaccctg ctgacggcgg acggcgaaga ggccggcatc
     5041 gtactcgaca gcgttcaaga ttcggaaaac tttaccgccc ttaccgtgat taaattctcc
     5101 gccgcacaaa aagaactcgc cgccccaaac ggcggtatct tcaaagcggt tcatctcttc
     5161 tttaaaaccg aaaatgcaga ataatccctc tgatgccgtc tgaagccctt cagacggcat
     5221 tgccgccccg tcaatcggtt acaatgccgc ctttctaatt ttcaaaccca aaccgccatg
     5281 tccaaaaaaa ccaaacaaga actcgaaaac aacaaactca gcaaacgcct gcgccacgcc
     5341 gtcggcgacg cgattaacga tttcaatatg atcgagccgg acgacaaaat catggtctgc
     5401 ctctccggcg gcaaagacag ctatgccctg ttagacatcc tgcgccagct ccaagccagc
     5461 gcgccgattg atttccaact ggttgccgtc aatctcgacc aaaagcagcc gggcttcccc
     5521 gaagaagtat tgccgaccta tctcgaaagc ataggcgtgc cgtacaaaat cgttgaagaa
     5581 gacacctact ccaccgtcaa acgcgtattg gacgaaggca aaacgacttg ttcgctgtgc
     5641 agccgcctgc gccgcggcat cctctaccgc actgcaaaag aattgggctg caccaaaatc
     5701 gccttgggac accaccgcga cgacatcctc gccaccttgt ttttaaatat gttttacggc
     5761 ggcaaactca aagccatgcc gcccaagctg gtgagcgaca acggcgaaca catcgtcatc
     5821 cgtccgctgg cgtatgtaaa agaaaaagac ttaatcaaat acgccgaatt aaaacaattc
     5881 cccatcatcc cgtgcaacct ctgcggctcg caacccaacc tgcaacgcca agtcatcggc
     5941 gatatgctgc gcgattggga caaacgcttc cccggccgca tcgaatcgat gttctccgcc
     6001 ctgcaaaacg tcgtcccttc ccaccttgcc gataccgaac tcttcgactt tgtcggattg
     6061 gaacgcggtc aaaccctgaa acacggcggc gatttggcgt ttgacagcga aaaaatgccc
     6121 gaacgttttt cagacggcag cgaagaagac gaaagcgaaa tcaaaatcga gccgcaaaaa
     6181 gccgaacgca aagtcatcaa cattctggcg aacaagccga aaacctgcgg cgcgtaaacc
     6241 tccaccgtca aaaatgccgt ctgaagcccg tttggcgttt cagacggcat tttaaaatcc
     6301 cgaagcagac ggcttccaaa catttgtttc tcagtgtccg ttttcagaat gcccgcctgc
     6361 tggcatattt tgcccgatca gttcggctat cctctcgccg tcaaactgcg ttttgaacac
     6421 caccttgatt tctttggaaa tctcggcaaa cagcttttcg gcatgtttga ttttccgctc
     6481 ttcgcttttt cgcaaatctt ccgccccgtc agtccctttg gcttcaatca caaagttcag
     6541 aatctcgccg cttttggttt tcacgatata ggcaaagtcg ggcgaatacg tgccgccgcc
     6601 ggcaacaggg attttgatgg agtttctcgg tattttggta aatacggtta cgccttcaat
     6661 ttggttgttg gcgacatttt catgttctat atccgaatcg tagaaaatct cgtcgaagag
     6721 atagccggcg acaggccggt gctccgtatc ttcaaatctg cccaaatctg cttttttcac
     6781 cgcgcgcggt ttgccgtctt tatcggtaaa tttggtcgga tggattttgc tgccgacaag
     6841 ctggtaatcc agttcgaatt tatggaagga atgatgaagc aaaaaccggt tgaagccgtt
     6901 tttgatttgg gcgatggtct gcatattcaa aaaatcgcca atgttcagtt cgttgcggac
     6961 gcggtaaaac gcctgatgca aagtctgcat gcggattttt gccgtttgtg ccagtttttc
     7021 cagaagctca cggtaagtca ttgtgttgaa acggataaaa tcttcatctt caaagctgtc
     7081 tatgcggcgg gaaagcataa gcccgttgtt gatataagct tcgtttaccg ccgtgcgtat
     7141 gcctgcctgc gggaatttgg cggcgttttc acgcaaatag gcagtaaata aatcgacaaa
     7201 ttcggcttca tctttgattt tgtactgcaa aacggcttta tggtgaatca gctcccacaa
     7261 ggctttgagt tcttcatatt tgccttcgcg catgatgatg gtgtctttgc cttcgtcttt
     7321 ggcgttgctg actttgcctt tgtccaaacc tttggggaag gcttcgggat aggcggcttt
     7381 taatttgtca tagccgtctt cggtaaagtt ttcattgtcg tcaatgatgc cgtctgaaaa
     7441 cagtttgttt aacaatccca gaggtttgat atcggggtat ttttgcaata ttttttgttt
     7501 cagctcttcg gtaaactttt ttgagatttc ttcctgaaaa gaattatcgt tgatttcgcc
     7561 gacaagctgc ttcacaaagt ctttttcgct gctatcgaca aaataattca gtttgtacgg
     7621 cacatcgcgc acccgcgcca tcagctcgtt taccggcagg cgcaggccgc gtccgacttc
     7681 ttgcagcttg gaagtcgtgc tgccgctgga acgcagtttg caaatctgga aaacgttggg
     7741 attgtcccag ccttcgcgca gcgtccattt ggaaaaaata aagcggcgcg ggttgtccaa
     7801 agacaacagt ttttccttat cgtgcaggat ttcattgatt tcctgctcga ttctatcgtc
     7861 gctgtctgta ttgtctttgg aaaaataacc gccgtggcag gcggatacat cgtccaacgt
     7921 cttttgcagg taatcgcggt aaaacgggtc gctttccgtt ttcaggcggc gtgccgcttc
     7981 cgcgtgaatc cagctttcaa atttatcttt caggctgcct gaaagctcgt tgccgctgcg
     8041 gtagcccgca atatcatcaa taaaaaacag cgtcagcggc ttgattttgg gctgtggcgc
     8101 gcgttctgcc aaaagtgcgc gttccagctt gaaatgttcg gcaaccgccc tctgcatcat
     8161 cgcatcctgc accgtttgcg aataggaata agggttgatg acggcgcccg ttttcaactc
     8221 caagccgttg cttaacacca ccacggtttt attcattttg tcgattttca aatccgaaac
     8281 agcaggatgg attttcgcca aatcttcgcc ttttgccagt ttgaacgtct gctttttgtc
     8341 cttttcgttt aattcaaatt tcgcttcttt gccgtccgac gacaccagtt ttaccgccgc
     8401 atccatgccg ccctgcattt cttcctgaaa cacgcgcacg cctttgacca gcccgtcgtt
     8461 aaacgcgcct accgccgtca aacggtaaag caggttgtaa tattcatcgt taaatgtcgc
     8521 accgtagcgc aaaatatatt gcggttttaa gcgtttgata ttgccccacg ttttcgcgct
     8581 ctcccgggtc gggaatttat gcggttcgtc cacaatcaca aacgggcgca cggcggccaa
     8641 tgcatcagcg ggattgtcaa acaaatcctt caatgccttg tcgcccgtat cggtcatgga
     8701 cgacgaatta accatgcccg cgttaatcag cagcacatgg atttcctttt tattttccgc
     8761 tttgacaaat tgctcaatcg ttatgggcgc attggacttt ttgcccttat tctttttcgc
     8821 gctttccacc acataggttt tcaggcgtac gccttcataa tcgccgccga aatcctgttc
     8881 aaaatgctct gccaaagcct tgctttgcaa aaactgccgc gttcccgcct taatggacaa
     8941 agtcggcaca accacgataa atttgaacac gcccagccaa cggtgcagct cgaacatggt
     9001 ttgtgtgtag gtataggttt tgcccgtgcc cgtttccatg gaaatatcaa ggatattttg
     9061 gtcgtccgaa cggtcgggga atcggccgtc tatgccgttt tggctttgca ctttcaggat
     9121 attgtttgcg tattgttttg cagcaaacaa aagttcggga ttttcgtctg ccgtccgata
     9181 tttgggcgtt gccccgtcaa acacgcccaa aactgccgaa accgcccgca tctggtgcgg
     9241 ctggtttttc tcgtaattaa aaccgctcat gaatgtttct ccataaagtc attgttgtat
     9301 cgtccttccc ccgcaagggg aatccaagcc gaaaagcagc ctgcactttc ggattcccag
     9361 ctacacccta ataaccacat tcaactcaat ctctttttta ttggtataac cgcgaaccgc
     9421 ctggtcgagt tcgtgctgca tggcgcttgc catattgctg ccgaatacaa tcacgcggtt
     9481 gggattgaaa tccgcatcgt cgtccagctt gcggataaac gccaacaaat cggcggaagt
     9541 aaaaccggca ttcatcagat acagccgttt ttcgcacaga tacgccgtgt aatcccctaa
     9601 ccgcacaggc tcaaccggcg tggtcagtgc cgccccgtca tacagcgtcc aggtggtcag
     9661 aagcgtttgc agctgttctt cgcttaattc atcgttaagc ggcaaatccg gttgttcggg
     9721 cgaaaaatcc ttgtccggat gctgcctgaa attgtctgcc gtttgaaaga ttttgaagcc
     9781 cgaatcgccc gtgtaatcgg gatgttcgac gcggattttg gcggcggctt tttctatgcg
     9841 ggctttggtg atgtcgaaga tggtcgggta gcctgcttta cgggcttcgg atttttcagc
     9901 ggttttttcg ggaagctgta cacagatata gcggcggtta ccgttttgtc cttcggcgtt
     9961 aagctgcatc acggcgtggg cggttgtgcc gctgcctgcg aagaagtcta ggattaggtc
    10021 attactcttt gaacttattg aaactaaaaa tttaatcaat tgactaggct tggggaaggt
    10081 aaatattttg ctaccaaata aatctgtgat ttcttttgtg ccttctttag tcattccgat
    10141 attttcaggt agcgtcctac taaaaatagc caaatattcg gctactgcat cggctaatgt
    10201 accaacattt tcaggtaatc tactgcttac agctactttg ccaaaatcct caccagcttt
    10261 tttcatgtcg tcatctttaa agtaacgcat aactggattg ctgatattta agaaatcata
    10321 atcatcaggg aaaacgattt ttcctttatt ataataatct tgaaatgtat ctttggttac
    10381 acgccaagtt gcatttggat ttgctggata tttttttcct gtcttgggat caaccattgt
    10441 gaaaaaacta tttggccttt ctgccgcagt tgtttgtttc gttaagtcgt gggtacgcca
    10501 aggacgatcg gggaaatcat cagtctcata atagcgtcgt tccttgcctt tagttgctgc
    10561 aataaattgg caagattttg cgaatacaaa tatccattca taatcctgcg aaataccaaa
    10621 aggcacatct gatttagctg ttctttttcg ccaaggcaat tgtgcaacaa aattcccttc
    10681 cccaaacact tcatcacaca acaatttcaa ctgcgccgct tcgttatcgt caatcgagat
    10741 aaaaatcacg ccgtcgtcct ttaacagttc gcgggcgata tacaggcgcg gatacataaa
    10801 ggtcagccac gcgctgtgcg agtttgagcc tttgtcggtg aaatctaaaa tccgcgcggc
    10861 ttcgtcttcg tcaatattcg ccaggagggc aagttcggcg ggtgtgaatt tgcggtcgtc
    10921 ctgatagaca aagccgtccg agccagtgtt gtagggcggg tcgatgtaaa tcatcttcac
    10981 gctgtttgta taggcgtttt ttaagtgttt caacacttct agattgtcgc cacgaatcag
    11041 caggttttgg ctgcctgcat tttcgggctt ggcgttgtgc gtcttatctt cacttatcag
    11101 ggtttcgggc ggcaggttgc gaagcaggcg ggcatatgat ttgcccagcc agttcatttc
    11161 gtaaaattcg cgtccgatgt cggtctgtgg cgcaatttcg gcttgtaatc tgtcgataag
    11221 gaaatttccg tctgcgtcaa aacaggcggg aaacagtttt ttgagctgtt cgagttgggt
    11281 agagttggcg gtaatgccgt ctgaagtgta gattgcctcg gtgttcgccc cggctgtgtc
    11341 gcttagatca
//
LOCUS       DAXHSF010000050        11158 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.050, whole genome shotgun sequence.
ACCESSION   DAXHSF010000050 DAXHSF010000000
VERSION     DAXHSF010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11158
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.050"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..338
                     /locus_tag="ACFM8S_000989"
     CDS             <1..338
                     /locus_tag="ACFM8S_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC6314074.1"
                     /translation="FFDTEPDRGKIEHCACLAMIHEEISAMPMGYETLIGDMGSALSG
                     GQKQRIVLARALYCEPKILFLDAATSHLDIANEKAVNANLNGLSIIKIMAAHRKETVE
                     SADRKMSLG"
     gene            complement(365..1585)
                     /locus_tag="ACFM8S_000990"
     CDS             complement(365..1585)
                     /locus_tag="ACFM8S_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HFC6314075.1"
                     /translation="MTFLQRLQGLADNKICAFAWFVVRRFDEERVPQAAASMTFTTLL
                     ALVPVLTVMVAVASIFPVFDRWSDSFVSFVNQTIVPQGADMVFDYINAFREQANRLTA
                     IGSVMLVVTSLMLIRTIDNTFNRIWRVNSQRPWMMQFLVYWALLTFGPLSLGVGISFM
                     VGSVQDAALASGAPQWSGALRTAATLTFMTLLLWGLYRFVPNRFVPARQAFVGALATA
                     FCLETARSLFTWYMGNFDGYRSIYGAFAAVPFFLLWLNLLWTLVLGGAVLTSSLSYWQ
                     GEAFRRGFDSRGRFDDVLKILLLLDAAQKEGKALPVQEFRRHINMGYDELGELLEKLA
                     RHGYIYSGRQGWVLKTGADSIELNELFKLFVYRPLPVERDHVNQAVDAVMTPCLQTLN
                     MTLAEFDAQAKKRQ"
     gene            complement(1600..2025)
                     /locus_tag="ACFM8S_000991"
     CDS             complement(1600..2025)
                     /locus_tag="ACFM8S_000991"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314076.1"
                     /translation="MPSEHAAYADLMASSAKRLLCAKAILFSSAVRTGAAVGNIFFDG
                     FADGGDFGDGAQYGFATFNAVDAASDSAGGIAGAAVLEIVEDEDFEGDLGSWADSVKM
                     VRSSDGITVVPMPSETAAVQAFVPDGKIGYNIGHQCPTY"
     gene            2130..2789
                     /gene="queC"
                     /locus_tag="ACFM8S_000992"
     CDS             2130..2789
                     /gene="queC"
                     /locus_tag="ACFM8S_000992"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFC6314077.1"
                     /translation="MSNQKALVIFSGGQDSTTCLIQAIQIYGRENVQAITFQYGQRHA
                     VELERARWIAQDLGVKQTVLDLSLMRQITHNALMDDTAAIETAENGVPNTFVDGRNAL
                     FLLYAAIYAKGQGIRHIIAGVCETDFSGYPDCRDVFVKSMNVTLNLAMDYDFQIHTPL
                     MYLTKAQTWALADEMGVLDYIREQTHTCYNGIVGGCRECPSCILRERGLAEYLESKKA
                     V"
     gene            2817..3335
                     /locus_tag="ACFM8S_000993"
     CDS             2817..3335
                     /locus_tag="ACFM8S_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HFC6314078.1"
                     /translation="MPKLHMFYLGGNAGRSNIEVHDIQFAVCDDYREAVPTLKAAWFG
                     DADKIHIDGWQVVEWADGYDIAVSETPKTEMPSEHAPRLYFANVGGYRAGQLAEAHAF
                     GLFAAATPAEAKQKALQTLLTDSYVQQHKDNLKDVDNLLALDRIGNFHIRLTPNPHGK
                     PAEIGFQGYLPI"
     gene            3342..3764
                     /gene="queD"
                     /locus_tag="ACFM8S_000994"
     CDS             3342..3764
                     /gene="queD"
                     /locus_tag="ACFM8S_000994"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFC6314079.1"
                     /translation="MKITKIFTFDSSHMLDGHDGKCQNLHGHTYKLEITVSDDPVRGG
                     PKDGMVMDFTDLKAIVKQHITDPFDHAFIYHGGNGRESQIAALLEGWNMKTLRLPCRT
                     TAENMAVEMYDRLKNAGLNVCRVKLWETPTSCAEYEGE"
     gene            4033..4404
                     /locus_tag="ACFM8S_000995"
     CDS             4033..4404
                     /locus_tag="ACFM8S_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFC6314080.1"
                     /translation="MKLLFILLVLFVAVEHFYIAWLEMTQIPSEKAAEIFKLPYEFME
                     QKQVQTLFSNQGLYNGFLGIGLVWSRFAAPDNAVYGATTLFLGFVLIAAAWGAFSSGN
                     KGILVKQGLPAMLAAAAVLAV"
     gene            4401..5039
                     /locus_tag="ACFM8S_000996"
     CDS             4401..5039
                     /locus_tag="ACFM8S_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFC6314081.1"
                     /translation="MKKINVAPENPQYRIVEIFESLQGEGWNTGMPAVFVRLGKCNLA
                     CGWCDTDYLTFGMMGLSDILGRLKTYAARNIIITGGEPTIQPHLDMLLDTLKAEGYFL
                     CLETNGLNPAPPQIDYVATSPKACYAAKYENSCIETADEVRIVADGDVLAFCENMERK
                     IRAHHYYLSPCEQDGAMNIYDTIRQIGILNSRPDAPVHWQLSVQTHKWAGIE"
     gene            complement(5376..5452)
                     /locus_tag="ACFM8S_000997"
     tRNA            complement(5376..5452)
                     /locus_tag="ACFM8S_000997"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5416..5418),aa:Pro,seq:cgg)
     gene            complement(5533..6618)
                     /gene="nagZ"
                     /locus_tag="ACFM8S_000998"
     CDS             complement(5533..6618)
                     /gene="nagZ"
                     /locus_tag="ACFM8S_000998"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFC6314082.1"
                     /translation="MTVPHIPRGPVMADIAAFRLTGEEKQRLLDPAVGGIILFRRNFQ
                     NIAQLKELTAEIKALRTPELIIAVDHEGGRVQRFIEGFTRLPAMNVLGQIWDKDGASA
                     AETAARQIGWVLATELSACGIDLSFTPVLDLDWGNCPVIGNRSFHRNPEAVTRLALAL
                     QKGLEKGGMKSCGKHFPGHGFVEGDSHLVLPEDGRSLSELETADLAPFRIMSREGMAA
                     VMPAHVVYPQVDTKPAGFSEIWLKQILRRDIGFKGVIFSDDLTMEGACGAGGIKERAR
                     ISFEAGCDIVLVCNRPDLVDELREDFRIPDNPALAQRWQYMANTLGSAAAQAVMQTTD
                     FQAAQAFVAGLASPQDTAGGVKVGEAF"
     gene            complement(6675..8195)
                     /locus_tag="ACFM8S_000999"
     CDS             complement(6675..8195)
                     /locus_tag="ACFM8S_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HFC6314083.1"
                     /translation="MQLIDYSHSFFSVVPPFLALALAVITRRVLLSLGIGILVGVAFL
                     VGGNPVDGLTHLKDMVVGLAWSDGDWSLGKPKILVFLILLGIFTSLLTYSGSNQAFAD
                     WAKRHIKNRRGAKMLTACLVFVTFIDDYFHSLAVGAIARPVTDKFKVSRAKLAYILDS
                     TAAPMCVLMPVSSWGASIIATLAGLLVTYKITEYTPMGTFVAMSLMNYYALFALIMVF
                     VVAWFSFDIGSMARFEQAALNEAHDETTVSDATKGRVYALIIPVLALIASTVSAMIYT
                     GAQASETFSILGAFENTDVNTSLVFGGTCGVLAVVLCTLGTIKTADYPKAVWQGAKSM
                     FGAIAILILAWLISTVVGEMHTGDYLSTLVAGNIHPGFLPVILFLLASVMAFATGTSW
                     GTFGIMLPIAAAMAVKVEPALIIPCMSAVMAGAVCGDHCSPISDTTILSSTGARCNHI
                     DHVTSQLPYALTVAAAAASGYLALGLTKSALLGFGTTGIVLAVLIFLLKDKKRANA"
     gene            complement(8396..9895)
                     /locus_tag="ACFM8S_001000"
     CDS             complement(8396..9895)
                     /locus_tag="ACFM8S_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HFC6314084.1"
                     /translation="MFKKYQYLALAALCAASLAGCDKAGSFFGADKKEASFVERIKHT
                     KDDGSVSMLLPDFVQLVQSEGPAVVNIQAAPAPRTQNGSGNAETDSDPLADSDPFYEF
                     FKRLVPNMPEIPQEEADDGGLNFGSGFIISKDGYILTNTHVVTGMGSIKVLLNDKREY
                     TAKLIGSDVQSDVALLKIDATEELPVVKIGNPKDLKPGEWVAAIGAPFGFDNSVTAGI
                     VSAKGRSLPNESYTPFIQTDVAINPGNSGGPLFNLKGQVVGINSQIYSRSGGFMGISF
                     AIPIDVAMNVAEQLKNTGKVQRGQLGVIIQEVSYGLAQSFGLDKAGGALITKILPGSP
                     AERAGLQAGDIILSLNGEEIRSSGDLPVMVGAITPGKEVSLGVWRKGEEITIKVKLGN
                     AAEHTGASSKTDEAPYTEQQSGTFSVESAGITLQTHTDSSGGHLVVVRVSDAAERAGL
                     RRGDEILAVGQVPVNDEAGFRKAMDKAGKNVPLLIMRRGNTLFIALNLQ"
     gene            complement(10033..10662)
                     /gene="nth"
                     /locus_tag="ACFM8S_001001"
     CDS             complement(10033..10662)
                     /gene="nth"
                     /locus_tag="ACFM8S_001001"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFC6314085.1"
                     /translation="MNRQIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATD
                     VGVNKATAKLFPVADTPQAMLDLGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYKG
                     EVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVE
                     DKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKA"
     gene            complement(10708..11115)
                     /locus_tag="ACFM8S_001002"
     CDS             complement(10708..11115)
                     /locus_tag="ACFM8S_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFC6314086.1"
                     /translation="MMPAYAALAFSAFTSATLLPGTSEAAFALFVHRFPEHAYGALLC
                     AGLANGLGSMVSYWMGRLLPSRKMPSEKTLNLMRRFGIWLLAFAWLPVVGDALPLAAG
                     WLRLNPWTSGLMLVVGKMARYAFILWGMQYYAA"
BASE COUNT         2607 a         3004 c         3112 g         2435 t
ORIGIN      
        1 gttttttcga tacggaaccg gatagaggaa aaatcgaaca ttgcgcctgc cttgctatga
       61 ttcacgaaga aatctccgcc atgcctatgg gctatgaaac cttgatcggc gatatgggca
      121 gcgcactgtc aggcggacaa aaacaacgca tcgtattggc gcgggcctta tattgcgaac
      181 cgaaaatcct atttttagat gcagcgacca gccatttgga tattgccaat gaaaaagcag
      241 tcaatgcaaa cttgaatggc ttgtctatca taaaaattat ggcggcacac agaaaggaaa
      301 cggtggaatc agcagatagg aaaatgtctt taggataaaa atacagtttc aaaaatactc
      361 aagactactg ccgttttttc gcctgagcgt caaactctgc cagcgtcatg ttcaaagtct
      421 gcaaacacgg tgtcattacc gcatcgacag cttggttcac atgatccctt tccacaggca
      481 acggacggta aacgaagagc ttgaagagtt cgttcaactc aatcgaatcc gcccccgttt
      541 tcaacaccca accctgtctg ccggaataga tgtagccgtg ccgcgccagc ttttccaaaa
      601 gctcgcccaa ctcgtcgtag cccatattga tatgccgtct gaactcctga acaggcaagg
      661 ctttgccttc tttttgcgcc gcatccagaa gcagcaggat tttcaacacg tcgtcaaacc
      721 gtccgcgcga gtcgaagccc ctgcggaacg cttctccctg ccagtaggag agtgaagaag
      781 tcagcaccgc gccgcccaag accagcgtcc acaacaggtt cagccacaac agaaaaaacg
      841 gcacggcggc aaacgcgccg taaatcgagc ggtagccgtc gaaattgccc atataccaag
      901 tgaagaggga gcgcgcggtt tccagacaaa acgctgttgc caaagccccg acaaacgcct
      961 gccgcgcggg aacgaagcgg tttggcacga agcggtacag cccccacagc aaaagcgtca
     1021 tgaaggtcag cgtcgccgcc gttcgcaacg cgcccgacca ctgcggcgca cctgaggcaa
     1081 gcgcggcatc ctgtaccgag ccgaccataa aggaaatgcc cacgcccaaa gacagcggcc
     1141 cgaacgtcag taaagcccaa tagacgagaa actgcatcat ccacggacgc tgggaattga
     1201 cccgccagat gcggttgaac gtattgtcta tcgtccgaat cagcatcagc gaggtaacga
     1261 ccagcatcac gctgccgatt gccgtcagcc ggttcgcctg ctcgcggaac gcattgatat
     1321 agtcgaacac catgtccgcg ccctgcggca caatggtttg attgacgaag gagacgaacg
     1381 aatccgacca gcggtcgaac acggggaaaa tcgaagcgac cgccaccatc acggtcagca
     1441 cggggacgag tgccagcagc gtcgtaaacg tcatgcttgc cgccgcctgc ggtacgcgtt
     1501 cttcatcaaa gcggcggacg acgaaccatg caaacgcaca gattttattg tctgccaaac
     1561 cttgcaaacg ttgtaaaaag gtcataattt cttgcccggt cagtaagttg ggcattgatg
     1621 cccgatgtta tagccaattt tgccgtcagg aacaaatgcc tgaactgcgg ctgtttcaga
     1681 cggcatcgga acaactgtta tgccgtctga agaccgaacc attttaacgg aatccgccca
     1741 tgaacccaaa tccccctcaa aatcctcgtc ctctactatt tccaaaacgg cagcacccgc
     1801 aatcccgccc gccgaatcgc tcgcggcatc gacagcgttg aaggttgcga agccgtattg
     1861 cgcaccgtcc ccaaagtctc cgccgtctgc gaagccgtca aaaaagatat tcccgacagc
     1921 ggctcccgtc ctgaccgccg aagaaaacaa tatcgccttc gcacaaagca aacgcttggc
     1981 ggaactcgcc atcaagtcgg cataagccgc gtgttcagac ggcatggcgt tcagatgccg
     2041 tctgaacgcg tttgcctgta taatccgcgt ctttactgtc caacttcgcg gttcgaaaac
     2101 ctcccgcgtc accaaaacta ggattcgata tgtcaaacca aaaagccttg gtcatctttt
     2161 cgggcggtca ggattcgacc acctgcctga ttcaggcaat ccaaatctac gggcgcgaaa
     2221 acgtccaagc cattactttc caatacgggc aacgccatgc cgtcgagctg gaacgtgccc
     2281 gctggattgc gcaggatttg ggcgtcaaac aaaccgtact cgacttgagc ctgatgcggc
     2341 agattacgca caatgccctg atggacgaca ccgccgccat cgaaactgcc gaaaacggcg
     2401 ttccgaatac ctttgtagac ggccgcaacg cgcttttcct gctctatgcc gcgatttacg
     2461 ccaaagggca ggggatacgg cacatcatcg cgggcgtgtg cgaaaccgac ttctccggct
     2521 atcctgactg ccgcgacgtg ttcgtcaaat ccatgaacgt tacccttaat ctggcgatgg
     2581 actatgattt ccaaatccac acaccgctga tgtatctgac caaggcgcaa acttgggcgt
     2641 tggcggacga aatgggcgtg ctggactata tccgcgagca aacccacacc tgctataacg
     2701 gcatcgtcgg cggctgccgc gaatgcccga gctgtatctt gcgcgaacgc gggctggcgg
     2761 aatatctgga aagtaaaaag gccgtctgaa cacgcgcaaa ccataaggaa tacgatatgc
     2821 ccaagctcca tatgttttac ctcggcggca atgccggcag gtcgaatatc gaagtgcacg
     2881 acatccaatt tgccgtgtgc gacgactacc gtgaagccgt ccccacactc aaagccgcat
     2941 ggttcggcga tgctgacaaa atccacatcg acggctggca ggttgtcgaa tgggcggacg
     3001 gttacgacat cgccgtatcc gaaacgccca aaacggaaat gccgtctgaa cacgccccgc
     3061 gcctgtattt tgccaatgtc ggcggttatc gcgcagggca gctcgccgaa gcgcacgctt
     3121 ttgggctgtt cgccgccgcc acgcctgccg aagccaaaca aaaagccctg caaaccctgt
     3181 tgaccgacag ctatgttcag caacataaag acaacttaaa agacgtggac aacctgcttg
     3241 cgctcgaccg catcggcaat ttccatatcc gcctgacccc gaatccgcac ggcaaacccg
     3301 ccgaaatcgg ctttcaaggc tatttgccca tttgagaacc catgaaaatc accaaaatct
     3361 tcaccttcga ctcctcgcat atgctcgacg ggcatgacgg caaatgccaa aacctgcacg
     3421 ggcataccta caaactcgaa atcaccgttt cagacgaccc cgtcagaggc gggccgaaag
     3481 acggtatggt gatggacttt accgacttga aagccattgt caaacaacac attaccgacc
     3541 ccttcgacca cgccttcatc taccacggcg gcaacgggcg cgaatctcaa atcgccgcac
     3601 tcttggaagg ctggaacatg aaaaccctgc gcctgccctg ccgcaccact gccgaaaata
     3661 tggcggtcga aatgtacgac cgtctgaaaa acgcggggct gaacgtgtgc cgcgtcaaat
     3721 tgtgggaaac gccgacatcg tgtgcggagt atgaagggga gtagggaata tcttgaacgt
     3781 atcgatatag taaattccaa taagacatgc ccaaccgcgt cattcccgcg caggcgggaa
     3841 tccagacctt gatttatcag gaatatttaa aaattgcagc aattccaact ctctggattc
     3901 ccgcctgcgc ggaaaggacg gtttagagcg tccttatttg aatttaccgt aaaacggttt
     3961 tttctcctgt acggattccc cgttttttca gacgaccttc catatcaaat acacccatta
     4021 aaaggaatac ccatgaaact cctcttcatc ctcctagtcc tcttcgtcgc cgtcgaacat
     4081 ttctacatcg cctggcttga aatgacacag attcccagcg aaaaagcggc ggaaatattc
     4141 aagctgcctt atgaatttat ggaacaaaag caagtgcaga ccttgttcag taatcaaggg
     4201 ctgtataacg gctttctcgg catcgggctg gtgtggtcgc ggtttgccgc gccggacaac
     4261 gccgtttacg gcgcgacgac tctgtttctc ggtttcgtat tgattgccgc cgcgtggggc
     4321 gcgttttcgt ccggcaacaa aggcatactc gtcaaacaag gactgcccgc gatgctggcg
     4381 gcggcagcgg tgttggcggt atgaaaaaaa tcaatgttgc ccccgaaaat ccgcaatacc
     4441 gtatcgtcga aattttcgag agcctgcaag gcgaaggctg gaacacgggc atgcccgccg
     4501 ttttcgtccg cttgggcaaa tgcaatctgg cgtgcggctg gtgtgatacc gattatttga
     4561 cattcggtat gatgggcttg tccgatatct taggccgtct gaaaacctac gccgcccgca
     4621 acatcatcat caccggcggc gagccgacca tacagccgca tctcgatatg ctgctggaca
     4681 cgctcaaggc ggaaggctat ttcctctgtc tcgaaaccaa cggactcaat cccgcgccgc
     4741 cgcaaatcga ctacgtcgcc accagcccca aagcctgcta cgccgccaaa tatgaaaata
     4801 gctgtatcga aacagccgac gaagtgcgga ttgttgccga tggtgatgtc cttgcgttct
     4861 gcgaaaacat ggaacgcaaa atccgcgcac atcattacta cctttcgccc tgtgagcaag
     4921 acggtgcgat gaacatctac gacaccatcc gccaaatcgg tattctgaac agccgccccg
     4981 acgcgccggt gcattggcag ttgagcgtgc agacgcacaa atgggcgggg atagagtagt
     5041 ttaagcagtg taactcaaag ggccggctta ccgttttgcc gatgtttgac atacggggaa
     5101 agtgtgccgc ttccgcgtga aactgccggc atttccgctg cctgattggg ggcggagcct
     5161 tactgaataa gatgccgtcg ttgggtacaa gcccggcttc ctaaattccg atggtctttt
     5221 gaaccttgcc gatactctgt gccagtgcgc gcaaatggca gggttggggg aaaacgaaat
     5281 gccgtctgaa acagcattct gtttcagacg gcatttttct gttgccgcca aaaggaaaaa
     5341 ccgtcccggc aatccatgcc gaggcggttt gaatatggtc ggaatgagag gattcgaacc
     5401 tccgacccct tcgtcccgaa cgaagtgcgc taccgggctg cgctacattc cgaattaagt
     5461 aaggcgtgat tatagcgcaa aaaatacggc gtgcctatac cgttttgcct ttttgccgcg
     5521 tgtcgggcgg atttaaaagg cctctccgac tttcacgccg cccgccgtgt cctgcggcga
     5581 ggcaaggccg gcaacaaagg cttgcgccgc ttggaaatcc gttgtttgca tcacggcttg
     5641 cgcggcggca ctgccgagcg tgttggccat atattgccaa cgttgcgcca aagcgggatt
     5701 gtcgggaatg cggaaatctt cgcgcagttc gtccacaagg tcgggacggt tgcagacgag
     5761 gacgatgtcg caacctgcct caaaggaaat gcgggcgcgt tctttgatgc cgcctgcccc
     5821 gcacgcgccc tccatagtca aatcatccga gaaaattacg cctttaaacc cgatgtcgcg
     5881 gcgcaaaatt tgtttgagcc agatttcgga aaaccctgcg ggctttgtgt ccacttgtgg
     5941 ataaacgacg tgggcgggca taaccgccgc cataccttcg cggctcataa tgcggaaggg
     6001 ggcgaggtcg gcggtttcaa gttcggacag gctgcgcccg tcttcgggca gcaccaaatg
     6061 gctgtctcct tcgacaaatc cgtgtccggg aaaatgtttg ccgcatgatt tcatgccgcc
     6121 tttttccaaa cctttttgaa gggcgagggc aaggcgggtt acggcttcgg ggttgcggtg
     6181 gaagctgcgg ttgccgatga cggggcagtt tccccagtcc aaatccaaga cgggagtgaa
     6241 ggacaagtcg atgccgcagg cggaaagttc ggttgccaaa acccagccta tctgccgggc
     6301 tgcggtttcg gcggcggacg cgccgtcttt gtcccaaatt tgccctaaaa cgttcattgc
     6361 gggcaggcgg gtaaaacctt cgatgaaacg ttgcaccctt ccgccttcgt gatcgacggc
     6421 gataatgagt tcgggtgtgc gcagggcttt gatttcggcg gtgagttctt tgagctgtgc
     6481 gatattttgg aagttgcggc ggaagaggat gatgccgcct acggcgggat cgagcaggcg
     6541 ttgtttttct tctccggtca ggcggaaggc ggcaatgtct gccatgacgg ggccgcgcgg
     6601 aatatggggg acggtcattg cggtttgctc caaaaagctt cagacggcat atgccgtctg
     6661 aacagggaaa ggggtcaggc gttggcgcgt tttttatctt tcaacagaaa aatcagcacc
     6721 gccaatacaa tgcctgtcgt gccaaagccc aacagcgcgg attttgtcag acccaatgcg
     6781 aggtagcccg atgcggcggc ggcggcaacg gttaaggcgt aaggcagttg cgaggtaacg
     6841 tggtcgatgt ggttgcagcg cgcgcctgtg gacgacagga tggtcgtgtc ggagatgggc
     6901 gaacagtggt cgccgcatac cgcccccgcc attactgcgg acatacacgg gataatcagc
     6961 gcgggttcga ctttgaccgc catggcggcg gcaatcggca gcatgatgcc gaacgtcccc
     7021 cagcttgtgc ctgtggcaaa cgccatcacg ctggcgagca ggaaaaggat gacgggcagg
     7081 aagccgggat ggatgttgcc cgcaaccagc gtggagaggt agtcgcccgt gtgcatttcg
     7141 ccgacaaccg tactgatgag ccaggcaagg attaaaatgg cgattgcgcc gaacatggat
     7201 ttcgcaccct gccaaacggc tttgggatag tcggcggttt taatcgtgcc gagcgtgcag
     7261 aggacgacgg caagcacacc gcaagtgccg ccgaagacca gggaagtgtt tacgtcggta
     7321 ttttcaaatg cccccaaaat gctgaaggtt tcgcttgcct gcgcgccggt gtagatcatg
     7381 gcggaaaccg ttgaggcgat taaggccaaa acgggaataa tcagtgcgta aacacgacct
     7441 ttggtagcgt ctgaaacggt agtttcatcg tgggcttcgt tcaacgcggc ttgttcgaaa
     7501 cgtgccatcg agccgatgtc gaaggaaaac catgcgacga cgaacaccat aatcagggca
     7561 aacagtgcgt aatagttcat caggctcatg gcgacaaacg tccccatcgg cgtgtattcg
     7621 gtgattttgt aggtaacgag cagtccggca agcgtggcga taatcgacgc gccccagctt
     7681 gaaacgggca tcagcacgca cataggcgcg gcagtggagt cgaggatgta ggcgagtttg
     7741 gcgcgggaaa ctttaaactt gtcggtaacg gggcgggcaa tcgcaccgac ggcgagactg
     7801 tggaaatagt cgtcgataaa ggttacaaac acgaggcagg cggtcagcat tttcgcgccg
     7861 cgccggtttt taatgtgccg ttttgcccag tcggcaaacg cctgattgct gccggagtag
     7921 gtcagcaggg aagtaaaaat acccaaaagt atcaggaaaa ccaagatttt tggtttgccc
     7981 agcgaccaat cgccgtctga ccaagccaag ccgacgacca tgtctttcag gtgtgtcaga
     8041 ccgtcgacgg ggttgccgcc gaccaaaaag gcaacgccga ccagaatacc gatgcctaaa
     8101 gacagcagta cgcggcgggt aatgacggca agtgccagtg ccaaaaaggg tggcacaacc
     8161 gagaaaaatg aatgtgaata gtcgatcagc tgcatggttt acgggggtgt taagcgtccg
     8221 gatggggcgg tttctgtccg caacggttcg gattttgttg gcaaaatggg cgaaagtatt
     8281 ttctgttccg gaaaatactt atttgaaatc aattaactga ttttttaaaa tgcccgttaa
     8341 tcggtattga cgggcatttt atcatttaaa aaatattttg gttaaattat gtgggttatt
     8401 gcaggtttaa tgcgataaac agcgtgttgc cacggcgcat gatcagcagg gggacgtttt
     8461 tgcctgcctt gtccatagct ttgcggaaac cggcttcgtc attgacgggg acttgcccga
     8521 cggcaagaat ttcgtcgccg cgcctcaagc ctgcgcgttc tgccgcgtcg gaaacccgta
     8581 cgacgacgag gtgtccgccg ctgctgtcgg tatgtgtctg aagggtaatg cctgcggatt
     8641 cgaccgagaa cgtaccggat tgctgttcgg tgtagggggc ttcatctgtt ttggatgatg
     8701 cgccggtatg ctcggcggcg ttgcccagct tgactttgat tgtgatttct tcgcctttgc
     8761 gccatacgcc gaggctgact tcttttcccg gcgtaatggc accgaccata acgggaaggt
     8821 cgccggaaga acgtatttct tctccgttga ggctgagtat gatgtcgcct gcctgcaagc
     8881 ctgcacgttc tgcggggctg ccgggaagga ttttggtaat caatgcgccg ccggctttgt
     8941 ccaaaccgaa cgattgtgcc aaaccgtagg atacttcttg aataatcacg cccagttgtc
     9001 cgcgttggac tttgccggtg tttttcagct gttcggcgac attcatggca acgtcaatcg
     9061 ggatggcgaa ggaaatgccc atgaatccgc cgctgcggct gtatatttgc gagttgatgc
     9121 cgacgacctg tccttttaag ttgaacagcg ggccgccgga gttgcccgga ttgatggcaa
     9181 cgtcggtttg gatgaagggt gtgtagcttt cgttgggcag gcttctgcct ttggcggaca
     9241 cgatgccggc ggtcacgctg ttgtcgaagc cgaagggcgc gccgatggcg gcgacccatt
     9301 cgcccggttt caaatctttg ggattgccga ttttgacgac gggcagctct tccgttgcgt
     9361 cgattttcag aagggcgaca tcggattgga catccgaacc gatgagtttg gcggtatatt
     9421 cgcgcttgtc gttgagcagg actttgatac tgcccatgcc ggtaacgacg tgcgtattgg
     9481 tcagaatata gccgtctttg ctgatgatga agcccgaacc gaagttcaat ccgccgtcat
     9541 ctgcttcttc ttgggggatt tcgggcatgt tcgggacgag gcgtttgaaa aattcgtaga
     9601 acgggtcgct gtcggcaagc gggtcggaat cggtttcggc attgccgctg ccgttttggg
     9661 tgcgcggggc gggggctgcc tgaatattga cgactgccgg gccttcgctt tgaaccagtt
     9721 ggacaaagtc gggcagcagc atactgacgc tgccgtcgtc tttggtgtgt ttgatgcgtt
     9781 ctacgaagga tgcttctttt ttgtccgcac cgaaaaagct gccggctttg tcgcagcctg
     9841 ccagcgaggc ggcacacagt gctgccaaag cgaggtattg gtattttttg aacacgtttt
     9901 gtcctttgtc ggatgccggt accggcttta atgccgtctg aagcgcattt tgtcggcttc
     9961 agacggcata ggttgaaatt ctacaacgtc cgtccgaatt ttcaagcgtt tcattttgaa
    10021 gggcggcggc agtcaggctt tggcgggata ttcgcacaaa tcgttgatga tgcaggtttg
    10081 gcattgcggt ttgagtgcct tgcaggtgta gcgtccgtgc aaaatcagcc agtggtgcgc
    10141 gtccatcaga aattctttag gaatgaagcg catcagtttg tcttcgactt cgcgcacatc
    10201 tttcccgggg gcgattttgg ttcggttgga cacgcggaaa atatgcgtat cgaccgccat
    10261 aacggggtgt ccgaacgctg tgttcaatac cacgtttgcc gttttgcgcc ccacgcccgg
    10321 caacgattcc aaagcctcgc ggtcttccgg cacttcgccc ttgtattttt ccagcaggat
    10381 gcggcaggtc tgcatgatgt gcttggactt ggttttatac aacccgatgg ttttcgtgta
    10441 ttccatcacg ccgtccaagc ccaaatccag cattgcctgc ggcgtatcgg caaccggaaa
    10501 cagcttcgcc gtcgccttgt ttacgccgac atcggtcgcc tgcgctgaaa gcagaacggc
    10561 gattaaaagc tcgaaagggg agttgaaatt cagctcggtg gtcggatggg ggttggcggc
    10621 gcggaaacgc tcgaagattt cttggcggat ttgtctgttc atttttttat acggtaggtt
    10681 tgtgcggtcg gcattataac gcacggttca ggcggcgtaa tattgcattc cccacaggat
    10741 aaaggcgtaa cgcgccatct tgccgacaac cagcatcagc ccgcttgtcc acggattcaa
    10801 ccgcagccag ccggcggcaa gcggcagtgc gtcgccgacg acgggcagcc aggcaaacgc
    10861 aagcagccaa ataccgaaac gccgcatcag attcagtgtt ttttcagacg gcatttttcg
    10921 ggaaggcagc aaacgtccca tccaatagga aaccatactg cccaatccgt tggcaaggcc
    10981 ggcgcacagc aacgcgccgt atgcgtgttc gggaaagcgg tggacgaaca gggcaaaggc
    11041 ggcttcggat gtgccgggca ggagggtggc ggaagtgaag gcggaaaagg cgagtgcggc
    11101 gtaggcgggc atcattgcaa acagtctcaa acaggcaaaa atcggcgacg gattgtac
//
LOCUS       DAXHSF010000051        10823 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.051, whole genome shotgun sequence.
ACCESSION   DAXHSF010000051 DAXHSF010000000
VERSION     DAXHSF010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10823
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.051"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(81..1226)
                     /gene="dapE"
                     /locus_tag="ACFM8S_001003"
     CDS             complement(81..1226)
                     /gene="dapE"
                     /locus_tag="ACFM8S_001003"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFC6314087.1"
                     /translation="MTETRSLELAKELIARPSVTPDDQNCQKLLAERLHKIGFAAEEL
                     HFGDTKNIWLRRGTKAPVVCFAGHTDVVPTGPVEKWDSPPFEPAERDGRLYGRGAADM
                     KTSIACFVTACERFVAEHPDHQGSIALLITSDEEGDALDGTTKVVDVLKARDELIDYC
                     IVGEPTAVDKLGDMIKNGRRGSLSGNLTVKGKQGHIAYPHLAINPVHTFAPALLELTQ
                     EVWDEGNKYFPPTSFQISNINGGTGATNVIPGELSVKFNFRFSTESTEAGLKQRVHAI
                     LDKHGVQYDLQWSCSGQPFLTQAGKLTDVARAAIAETCGIEAELSTTGGTSDGRFIKA
                     IAKELIELGPSNATIHQINENVRLDDIPKLSAVYEGILARLLAGNAV"
     gene            complement(1326..1946)
                     /locus_tag="ACFM8S_001004"
     CDS             complement(1326..1946)
                     /locus_tag="ACFM8S_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFC6314088.1"
                     /translation="MLTSKSCDLFNIPFFQFSQLKKYQPESIPQIKADYKENWQVWQQ
                     LIQQVAAELGAPFAPPHIERWCNGWQVRAHFFAYFKYEQYKNSAAILSILLNRRRLSV
                     SLDWHCYKADVSPIALPEYNRWLDDFDTDKYAAFDMWHGAESEYDDYRTVAQQSESDR
                     KLQNDEDFFCIGKHIERDDLGKQDVAKWIAETVEDLLPLYEACHGA"
     gene            complement(1971..2480)
                     /locus_tag="ACFM8S_001005"
     CDS             complement(1971..2480)
                     /locus_tag="ACFM8S_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HFC6314089.1"
                     /translation="MNPFETKSVTFAEPIEMLYACHGKVRRFCGQVAMLSDYIAENGC
                     NQIVLQTIRQITRYFNVAAPLHHEDEEEDFFPLLLQYAPQAREGVDELLRQHIGLHDN
                     WAAVSAEFAKLEADNAYVPDAEAFKRFVAGYDVHLAIEEPLFDMGNTFIPKEKLTEIG
                     EIMAARRRK"
     gene            complement(2575..3126)
                     /gene="azu"
                     /locus_tag="ACFM8S_001006"
     CDS             complement(2575..3126)
                     /gene="azu"
                     /locus_tag="ACFM8S_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HFC6314090.1"
                     /translation="MKAYLALISAAVIGLAACSQEPAAPAAEATPAAEAPASEAPAAE
                     AAPADAAEAPAVGNCAATVESNDNMQFNTKDIQVSKACKEFTITLKHTGTQPKASMGH
                     NLVIAKAEDMDGVFKDGVGAADTDYVKPDDARVVAHTKLIGGGEEASLTLDPAKLADG
                     EYKFACTFPGHGALMNGKVTLVD"
     gene            complement(3407..3829)
                     /locus_tag="ACFM8S_001007"
     CDS             complement(3407..3829)
                     /locus_tag="ACFM8S_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HFC6314091.1"
                     /translation="MNLEQLGRRDALLSGLLKQAGQWRRLDAAVKKLLPANLHPHFQT
                     VCIEDGRLVLLAANNMAASRLKMIAPSVLPQLAGLDASIRSVSVRLVPKPEKPPKTNT
                     LHLSKAALESFGSAAVKLEKRHPELAEALANLVRRHGA"
     gene            3960..6710
                     /gene="secA"
                     /locus_tag="ACFM8S_001008"
     CDS             3960..6710
                     /gene="secA"
                     /locus_tag="ACFM8S_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFC6314092.1"
                     /translation="MLTNIAKKIFGSRNDRLLKQYRKSVARINALEEQMQALSDADLQ
                     AKTAEFKQRLADGQTLDGILPEAFAVCREASRRTLGMRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLASRDAGIMEPLYNFLGLTVGVIISD
                     MQPFDRQNAYAADITYGTNNEFGFDYLRDNMVTDQYDKVQRELNFAVVDEVDSILIDE
                     ARTPLIISGQADDNIQLYQIMNTVPPHLVRQETEEGEGDYWVDEKAHQVILSEAGHEH
                     AEQILTQMGLLAENDSLYSAANIALMHHLMAALRAHSLFHKDQHYVIQDGEIVIVDEF
                     TGRLMSGRRWSEGLHQAVEAKEGVEIKRENQTLASITFQNYFRLYTKLSGMTGTADTE
                     AFEFQSIYNLETVIIPTNRPVQRKDFNDQIFRSAEEKFEAVVKDIEECHKRGQPVLVG
                     TTSIENSELVSHLLQKAGLPHNVLNAKEHEREALIVAQAGKVGAITVATNMAGRGTDI
                     VLGGNLKHQTDAIRADETLSDEEKQAQIAALENGWQAEHDKVMEAGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSFEDPLLRLFALDRAAAILNRLAPERGVAIEHNL
                     LTRQIEGAQRKVEGRNFDMRKQVLEYDDVANEQRKVIYSQRNEILTSKDISDLMQEIR
                     SDVVSDLVDTYMPPDSMEEQWDIPTLENRLAAEFRLHEDIQSWLKADNAIDGQDIKER
                     LIERIENEYAAKTELVGKQAMADFERNVMLQVIDNQWREHLAAMDYLRQGIHLRSYAQ
                     KNPKQEYKREAFTMFQDLWNGIKFHIASLLTSVQIEQNPVAVVEEQPIGNIQSIHSES
                     PDMEELLGQSQTDLVTEAFNPDGTDFSPEALEARGQIVHRNDPCPCGSGLKYKQCHGK
                     LA"
     gene            6854..8626
                     /gene="dnaG"
                     /locus_tag="ACFM8S_001009"
     CDS             6854..8626
                     /gene="dnaG"
                     /locus_tag="ACFM8S_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFC6314093.1"
                     /translation="MIPSDFIDELLAKTDIVDIIDEQVPLKKGGANYMACCPFHKEKT
                     PSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMVVPKVRGQND
                     NPEVRAERKKKQQTLEETTAAAADFYAQQLKFNPAAKAYLDKRGLSAEVIAHYGLGYA
                     PDGWQPLAQVFQPYPNTALVDTGMVIDNEGRYYDRFRHRIMFPIRNPRGQVIGFGGRV
                     LDDSKPKYLNSPDTPLFDKGKNLYGLYEGRTAVKEAGRILVVEGYMDVVALAQFGVGY
                     GVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFL
                     FLPEEHDPDSYIRAYGKAQFEDALLNQSKPLSEYFWEHLSDGIHLNTQEGKAELVKTS
                     SPLLAQITAPALAYLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQ
                     PVMPTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAETIKNHPSVPATAQV
                     LEHMRGSPYEETINRIFRSALQSEEMEGGGEEDCENFQIGIKKLLNELKYSQIETLKQ
                     KSLQSGLNESEKKLLLSLLTAKQN"
     gene            8813..10741
                     /gene="rpoD"
                     /locus_tag="ACFM8S_001010"
     CDS             8813..10741
                     /gene="rpoD"
                     /locus_tag="ACFM8S_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFC6314094.1"
                     /translation="MSKNQNHEEYQDDARPLTIEEQRARLRQLIIMGKERGYITYSEI
                     NDALPDDMSDADQIDNIVSMISGLGIQVTEHAPDAEDILLSDNAAVTDDDAVEEAEAA
                     LSSADSEFGRTTDPVRMYMREMGQVDLLTREDEIIIAKKIENALKNMVQAISACPGSI
                     AEILELIEKIRKDEIRVDEVVEAIIDPNEVLLNELGLGHLETTAPEKPSNDNSDENED
                     DEESEEDADEISAANLAELKQKVIGHFAQIEKDYKKMIGRLEKHHSRHKDYLAYRDAI
                     ANKLLEVRFATRQIDSLSSSLRRKVENIRKLEREIRDICLDRVHMERDYFIQNFLPEI
                     TNLEWIEEEIAKGRVWSDALDRFRHAILEKQTELADMEKETRISIEELKEINKNMVSS
                     EKETAAAKQEMIQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYK
                     FSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQETGEEPDSAKLA
                     ELMQMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDANNVAPADAAMYTSLHE
                     VTKEILESLTPREAKVLRMRFGIDMNTDHTLEEVGRQFDVTRERIRQIEAKALRKLRH
                     PTRSDRLRSFLDSEDSKL"
BASE COUNT         2825 a         3020 c         2672 g         2306 t
ORIGIN      
        1 aatttaatcc actatatctc aaacccacat taggtctaat caaatggtcg gacatccata
       61 tccgacagcc cattttcttt tcagacggca tttccagcca acaagcgcgc caatatcccc
      121 tcatacaccg ccgacagctt cggaatatcg tccagccgca cgttttcgtt gatttggtgg
      181 atggtcgcat tggacgggcc taattcgatg agttcttttg caatggcttt gatgaagcgt
      241 ccgtccgaag tgccgccggt ggtggacaat tcggcctcaa tgccgcaggt ttcggcaatg
      301 gctgcgcgtg ccacgtcggt cagtttgccc gcttgggtca gaaagggctg ccccgaacac
      361 gaccactgta aatcgtattg cacgccgtgt ttgtccaaaa tggcgtgaac gcgttgtttc
      421 agccctgctt cggtggactc ggtggagaag cggaaattga atttgacgct cagctcgccc
      481 ggaatgacat tggtcgcgcc tgtaccgccg ttgatattgg aaatttgaaa gctggtcggc
      541 gggaagtatt tgttgccttc gtcccagact tcctgcgtca gctctaacaa ggccggggca
      601 aaagtatgca cgggattgat tgccaaatgc ggataggcaa tatggccttg cttgcctttg
      661 acggtcaggt tgcccgacag cgagccgcgc cggccgtttt taatcatatc gcccaatttg
      721 tccacggctg tcggttcgcc gacgatgcag tagtcgataa gctcgtcgcg cgctttcaat
      781 acatcgacga ctttggtcgt gccgtccaac gcgtcgccct cttcgtcgga agtaatcaga
      841 agcgcaatgc tgccttggtg gtcgggatgt tcggcaacga agcgttcaca ggcggtaacg
      901 aagcaggcga tgctggtttt catgtctgcc gcgccgcgcc cgtataatct tccgtcgcgc
      961 tcggccggtt cgaacggggg cgaatcccat ttttcgacag gacctgtcgg tacaacgtcg
     1021 gtatgccctg caaaacagac gacgggagct ttcgtgccgc gtcgcaacca gatgtttttg
     1081 gtgtcgccga aatggagttc ttcagccgca aaaccgattt tgtgcaggcg ttcggcaagc
     1141 agtttttggc aattttggtc atccggcgta acggacggtc gggcaatcaa ttctttggca
     1201 agctctaggg atcgggtttc ggtcattttt cggcttttct gtttgagatg ccgtctgaac
     1261 ggcgtttcag acggcatttg atgttgaatg gaacgggcta acgatagcga agcgggttct
     1321 tcaggtcaag caccatgaca cgcttcgtaa agtggcagca aatcttccac cgtttccgct
     1381 atccatttcg cgacatcctg cttgcccaaa tcgtcgcgct cgatgtgttt gccgatgcag
     1441 aaaaagtctt cgtcgttttg caactttcgg tcggattcgc tttgttgtgc gacggtgcgg
     1501 tagtcgtcat attcgctttc cgcgccgtgc cacatatcga atgcggcgta tttgtcggta
     1561 tcgaaatcat ccaaccagcg gttgtattcg ggcagcgcaa tcggcgacac gtcggctttg
     1621 tagcagtgcc aatccaagct gacgctcagg cggcggcggt tcaataaaat cgataaaatc
     1681 gcggcggaat ttttatattg ttcgtatttg aagtaggcaa agaaatgggc gcgcacctgc
     1741 cagccgttac accagcgttc gatatgcggc ggcgcgaacg gcgcacccaa ttcggcggca
     1801 acctgctgga tcagctgctg ccatacctgc cagttttctt tatagtctgc cttgatttgc
     1861 ggaatgcttt caggctggta ttttttaagc tgggaaaatt ggaaaaacgg gatattgaac
     1921 aaatcgcaac ttttcgaggt aagcatagtg tattccttga gacgattacc ttatttgcgc
     1981 cgacgcgccg ccatgatttc accgatttcg gtcagttttt ctttagggat aaaggtgttg
     2041 cccatatcaa acagcggctc ttcaatcgcc aaatgaacat catatcccgc cacaaaacgt
     2101 ttgaacgctt ccgcatcggg aacataagca ttgtctgctt cgagcttggc aaattcggcg
     2161 gaaacagccg cccagttgtc gtgcagcccg atatgttggc gtaaaagctc gtccacacct
     2221 tcacgtgctt gcggcgcgta ttgcagcagc agcgggaaaa agtcttcttc ttcgtcctca
     2281 tgatgcagcg gcgcggcaac gttgaaatac cgggtgattt ggcggatggt ttgcaaaaca
     2341 atttgattgc agccgttttc ggcgatgtag tccgacagca tggcgacttg tccgcaaaaa
     2401 cggcgcactt tgccgtggca ggcatacagc atttcaatcg gttcggcaaa ggtaacgctt
     2461 ttggtttcaa acggattcat gttttcgttc tcaacgggca cttttcaagc agtcatttta
     2521 taataaaaca gcctgcacaa agcaggctgt ccgtcttttg agactttaag cggattaatc
     2581 gaccaaagtc actttgccgt tcatcaaagc accgtgacct gggaaggtac aagcgaattt
     2641 atattcgccg tcggccaatt tagcaggatc cagagtcagg gaagcttctt cgccgccgcc
     2701 gatcagtttg gtatgggcaa caacgcgtgc atcatcaggt ttgacatagt cggtatcggc
     2761 agcacctacg ccgtctttaa atacgccgtc catgtcttca gctttggcaa tcacgagatt
     2821 gtgacccatg ctggctttgg gttgcgtacc ggtatgtttc agagtgatgg taaactcttt
     2881 acatgctttg ctgacttgga tgtctttggt gttgaactgc atattgtcgt tggattcgac
     2941 agttgccgca cagttgccga cagcaggggc ttcggcagca tctgcaggag cagcttcggc
     3001 ggcaggtgct tcggaagcgg gtgcttcagc agcaggagtt gcctcggcag caggcgcggc
     3061 aggttcttga gagcaggcag ccaaaccgat aacggcagca gaaatcagag ccagatacgc
     3121 tttcataaca aatctccaat cgataaaata atattcggtt ttacagaaat caaagtgcaa
     3181 ccgccattaa caaaaccttg aaaaagattc cgccgcgttg cacaaacaga tgtttcggag
     3241 cggcattttg ctacaaattt catttgaaat caaagcctgt ctgcaagttt acaatcgttt
     3301 actttaaaag gggcaatttt accccgaacc tatttcttta gtattagacc cattatcctt
     3361 tacttcttaa tattaacgga tgtttacaca aattcccgta tacattttat gcgccatgcc
     3421 ttctaaccaa gtttgccaat gcctccgcca attcgggatg ccgtttttcc aactttaccg
     3481 ccgccgaacc gaaactctcc agcgcagcct tactcaaatg cagggtattg gttttcggcg
     3541 gtttttccgg tttcgggacc agcctgaccg aaacagagcg tatcgaagcg tcaagccctg
     3601 ccaactgcgg caataccgac ggtgcaatca ttttcaagcg cgatgccgcc atattgtttg
     3661 ccgccaaaag gacaagcctg ccgtcttcga tacataccgt ctgaaaatgc gggtgcaggt
     3721 tggcaggcag cagttttttc acggcggcat ccaaccgccg ccactgtccc gcctgtttca
     3781 aaagtccgga aagcagcgcg tcccgcctgc ccaactgttc caaattcata aaacatacac
     3841 ccaaaaagat tgaaataccg caaacgcgcc tttatttcag acggcattag cactttgcac
     3901 aaacgcttgt gttaaaatcg cgttttcgcc cactattata tcaggcgcag gaattattca
     3961 tgctgacaaa cattgccaag aaaatcttcg gcagccgcaa cgaccgcttg ctgaaacaat
     4021 accgtaaatc cgttgccaga atcaacgcgc tcgaagaaca gatgcaagcc ctaagcgatg
     4081 ctgatctgca agccaaaact gccgaattca aacaacgcct cgccgacggt cagactttgg
     4141 acggcatttt gcccgaagcc ttcgccgtct gccgcgaagc gtcccgccgc accctcggta
     4201 tgcgccactt cgacgtgcag cttatcggcg gtatggtgct gcacgacggc aaaatcgccg
     4261 aaatgcgtac cggcgaaggc aaaaccctgg tcgccaccct cgccgtctat ctcaacgcgc
     4321 tggccggcaa aggcgtacac gtcgttaccg tcaacgacta cctcgcctca cgcgatgcgg
     4381 gcattatgga gccgctctac aatttcctcg gccttaccgt gggcgtgatt atttcagata
     4441 tgcagccgtt cgaccgtcaa aacgcctatg ccgccgatat cacctacggc accaataatg
     4501 aattcggctt cgactacctg cgcgacaata tggttaccga ccaatacgac aaagtgcagc
     4561 gcgaattgaa ttttgccgtt gtcgatgaag tggattccat cttgattgac gaagcgcgca
     4621 ctccgctgat tatctccggt caggcggatg acaacatcca gttgtaccaa atcatgaaca
     4681 ccgttccgcc ccacctcgtc cgtcaagaga cagaagaagg cgaaggcgac tattgggtcg
     4741 acgaaaaggc acatcaggtc atcctgagcg aagcaggtca cgaacacgcc gagcaaatcc
     4801 tgacccaaat gggattgctg gcagaaaacg actccctcta ttccgccgcc aatatcgccc
     4861 tgatgcacca ccttatggcg gcattgcgcg cgcattccct cttccacaaa gaccaacatt
     4921 acgtcatcca agacggcgaa atcgtcatcg tggacgaatt caccggccgg ctgatgtccg
     4981 gccgccgctg gtcggagggt ctgcatcaag ccgtcgaagc caaagaaggc gtggaaatca
     5041 aacgcgaaaa ccaaaccctt gcctccatca ccttccaaaa ctatttccgc ctgtacacca
     5101 agctctccgg catgaccggc acagccgata ccgaagcttt cgagttccaa agcatctaca
     5161 acctcgaaac cgtcatcatc ccgaccaacc gccctgtaca gcgcaaagac ttcaacgacc
     5221 agattttccg ttccgccgaa gaaaaattcg aagccgtcgt caaagacatc gaagaatgcc
     5281 acaaacgcgg gcagcccgtc ctcgtcggca ccaccagcat cgaaaactcc gaactggtct
     5341 cccaccttct gcaaaaagcc ggactgccgc acaacgtcct caacgccaaa gaacacgaac
     5401 gcgaagccct gattgtcgcc caagccggca aagtcggcgc gattaccgtt gccaccaata
     5461 tggcgggacg cggtacggac atcgttttag gcggcaacct gaagcaccaa accgatgcca
     5521 tccgtgccga cgaaaccttg agcgacgaag agaaacaggc acaaatcgcc gcactcgaaa
     5581 acggctggca ggcagaacac gataaagtaa tggaagcagg cggcctgcac atcatcggta
     5641 cggaacgcca cgaaagccgc cgtatcgaca accaattgcg cggacgttcc ggccgtcagg
     5701 gcgaccccgg atccagccgt ttctaccttt cgtttgaaga cccgctgctg cgcttattcg
     5761 cactcgaccg cgccgccgcc atcctcaacc gtctcgcccc cgaacgcggc gtcgccatcg
     5821 aacacaacct gctgacgcgc caaatcgaag gggcgcaacg caaagtcgaa ggcagaaact
     5881 tcgatatgcg caaacaggtt ttggaatacg acgacgttgc caacgaacag cgcaaagtca
     5941 tttacagcca gcgcaacgaa attctgacca gcaaagacat cagcgacctg atgcaggaaa
     6001 tccgttctga tgtcgtcagc gacctcgtgg atacctatat gccgcccgac agcatggaag
     6061 aacaatggga catcccgact ttggagaacc gtctggctgc cgaattcaga ctgcacgaag
     6121 acatccaatc ctggctgaag gcggacaatg cgattgacgg tcaagacatc aaagaacgcc
     6181 tgatcgaacg catcgaaaac gaatatgccg ccaaaaccga actggtcggc aagcaggcaa
     6241 tggccgattt cgagcgcaac gtgatgttgc aggtcatcga caaccaatgg cgcgaacacc
     6301 tcgccgctat ggactacctg cgacaaggca tacacctgcg cagctatgcc caaaaaaatc
     6361 cgaagcagga atataaacgt gaagccttta ccatgttcca agacctgtgg aacggcatca
     6421 aattccatat tgcctccctg cttacctcgg ttcaaatcga acaaaaccct gtcgcggtgg
     6481 ttgaagagca acccatcggc aacatccagt ccatccattc cgaatcgccc gatatggaag
     6541 aacttttggg tcagtcgcaa accgatttgg ttaccgaggc ctttaatccc gatggaacgg
     6601 atttcagccc cgaagccttg gaagcgcggg gacaaatcgt ccaccgcaac gacccctgcc
     6661 cctgcggcag cggtttgaaa tacaaacaat gccacggcaa actggcttga gcgtttgaac
     6721 gcaaatgccg tctgaacatc cggtttccgg cttcagacgg cattttgcct gaaccgccac
     6781 atccgaccgc cattccgaaa aatcccgatt tcgtaccgtc caaactaaaa acaggcatcc
     6841 gccccgccga actatgattc catccgactt cattgacgaa cttctggcca aaaccgatat
     6901 tgtcgatatt atcgacgagc aggttccgct gaaaaaaggc ggggcgaact atatggcgtg
     6961 ttgcccgttc cacaaggaaa aaacgccgtc gttttcggtc agtccaacca agcagtttta
     7021 ccattgtttc agttgcgggg cgcatggttc ggcgattggt tttgtgatgg agcatcaggg
     7081 gctgtcgttt ccggaggctg tgcagtttct tgccgaccgt gtgggcatgg ttgtgcctaa
     7141 agtgcgcggg cagaacgata atcccgaagt ccgtgccgaa cgtaagaaaa aacagcagac
     7201 gctggaagaa acgacggcgg cggcggctga tttttacgcg caacagttga aattcaatcc
     7261 ggcggcaaaa gcttatttgg acaagcgcgg tttgagtgcg gaagtcatcg cgcattatgg
     7321 tttgggctat gcgcccgacg gctggcagcc tttggcgcaa gtgttccaac cgtacccgaa
     7381 taccgcgttg gtggatacgg gtatggtgat tgacaatgag gggcggtatt acgaccgatt
     7441 ccgtcatcgg attatgtttc ctatccgcaa tccgcgcggg caggtcatcg gtttcggcgg
     7501 cagggtgttg gacgactcca aacccaaata tctaaactct cccgatacac ctttgtttga
     7561 caaagggaaa aacctttacg gcttgtatga ggggcgtacc gctgtcaagg aagcggggcg
     7621 gattctggtg gtcgaaggct atatggacgt ggtcgcgctg gcacagttcg gcgtgggcta
     7681 cggtgtggcg gccttgggca cggcaacaac agcggaacac gtcaaaatcc tgatgcgtca
     7741 ggcggacagt atttatttct gtttcgacgg cgacagcgcg gggcgcaaag cggcttggcg
     7801 cgcgctggaa aacgcgctgc cgcagttgaa ggacgacaaa tcgctgcatt ttttgttcct
     7861 gccggaagaa cacgaccccg acagttacat ccgtgcctac ggcaaagcgc aatttgaaga
     7921 tgcgctgttg aatcaaagca agcccttgtc ggaatatttc tgggaacacc tttcagacgg
     7981 cattcatctc aatacgcagg aaggcaaggc ggaattggtg aaaaccagtt cgccgctttt
     8041 ggcgcagatt accgcgccgg cattggctta tttgttaaaa caacggctta gcgagctggt
     8101 cggcatcgac cccgacaacc tcgcgcaact gctgggacag gaagcgccga agcggcacgt
     8161 caaacaaaaa aactacaaac tgccgccgat ttccgtcaaa cagcccgtta tgccgacatt
     8221 ggtgcaacgg caaatccgca gcctcttgat aaatccggat tgggctgcat atatagacct
     8281 gcccgattat ctggcgttgg acggtgattt cgcctgcctt gccaacctcg ccgaaaccat
     8341 taaaaaccat ccttccgtgc cggcaaccgc acaggttttg gaacatatgc gcggttcgcc
     8401 ctacgaagaa acaatcaacc gcatcttccg gtcggccctt caatcggaag aaatggaagg
     8461 cggcggcgaa gaagattgcg aaaacttcca aatcggcatc aaaaaactgc tcaatgagtt
     8521 aaaatacagc caaatcgaaa cattaaaaca aaaaagcctg caatccggct taaatgaaag
     8581 cgagaaaaaa cttttgctgt cgctgctgac cgcaaaacaa aattgaccgg cggattccgc
     8641 catccgtaaa ccgttatgcc gtctgaaaag cattcacccc ggctgcaaca acgacacctg
     8701 cagaacaccc atccccaaaa gccttcagac ggcatcagag taccctactc tgccacgcct
     8761 tcaggtgcgt ccaaacgcaa accgtcggca tcttgccaac agaaagcaga caatgtccaa
     8821 aaaccaaaat cacgaagaat accaagacga tgcccgcccc ctgacgattg aggaacaacg
     8881 cgcgcgcctg cgccaactca tcattatggg caaagaacgc ggctacatca cctactccga
     8941 aatcaatgac gcactgccag acgatatgtc tgatgccgac caaatagaca atatcgtcag
     9001 catgatttcc ggtttgggta tccaagttac cgaacacgcc cccgatgcgg aagacatatt
     9061 gttaagcgac aatgccgccg ttaccgacga tgatgccgtc gaagaagccg aggccgccct
     9121 ttccagcgcc gactccgagt tcggcaggac gaccgacccc gtccgtatgt atatgcgcga
     9181 aatgggacag gtcgacctgc tgacccgcga agacgaaatc atcatcgcca aaaaaatcga
     9241 aaacgccctg aaaaatatgg ttcaggccat ctccgcctgc ccgggatcca ttgcggaaat
     9301 cttagaactc atcgaaaaaa tccgcaaaga cgaaatccgc gtcgacgaag tcgtagaagc
     9361 cattatcgac ccgaatgaag tattgctcaa cgaattgggc ttggggcact tggaaaccac
     9421 agcacccgag aaaccttcca acgacaattc ggacgaaaac gaagacgacg aagaatcgga
     9481 agaagatgcg gatgaaatct cggcagccaa tctcgccgaa ttgaaacaaa aagtcatcgg
     9541 ccactttgcc caaatcgaaa aagactacaa aaaaatgatc ggccgtttgg aaaaacacca
     9601 cagccggcac aaagactatc tcgcctaccg cgacgcgatt gccaacaaac tgctggaagt
     9661 ccgtttcgcc acccggcaaa tcgacagcct cagcagcagc ctgcgcagaa aagtagaaaa
     9721 catccgcaaa ctcgaacgcg aaatccgcga catctgcctc gaccgcgtcc atatggagcg
     9781 cgactacttc atccaaaact tcctgcccga aatcaccaat ctggaatgga ttgaagaaga
     9841 aatcgccaaa ggcagggttt ggagcgacgc gctcgaccgc ttccgccacg ccatcctcga
     9901 aaaacaaacc gagttggcgg atatggaaaa agaaacccgc atttccatcg aagagttgaa
     9961 agaaatcaac aaaaatatgg tgtcgagcga aaaagaaacc gcagccgcca aacaggaaat
    10021 gattcaggca aacttgcgcc tcgtcatttc cattgccaaa aaatacacca accgtggctt
    10081 gcagttcctt gatttgattc aggaaggcaa catcggcctg atgaaagcgg tcgacaagtt
    10141 cgaataccgc cgaggctata agttctccac ctacgcaacc tggtggatcc gtcaggcaat
    10201 cacccgctcg attgccgatc aggcgcgtac catccgcatt ccggtacata tgattgaaac
    10261 catcaacaag atgaaccgca tctcgcgcca acaccttcaa gaaaccggcg aagaacccga
    10321 ttccgccaaa cttgccgaac tgatgcagat gcccgaagac aaaatccgca aaatcatgaa
    10381 aatcgccaaa gagccgattt cgatggaaac ccccatcggc gacgacgacg attcgcactt
    10441 gggcgacttc atcgaagatg ccaacaatgt tgcgccggcc gatgcggcaa tgtacaccag
    10501 cctgcacgaa gtaaccaaag aaatcctcga aagcctgaca ccgcgtgagg caaaagtcct
    10561 gcgtatgcgt ttcggcatcg atatgaacac cgaccacacg ctggaagaag tcggcagaca
    10621 gtttgacgta acgcgcgaac gcatccgaca aatcgaggca aaagcactcc gcaaattacg
    10681 ccatccgaca agaagcgacc gtttgagaag tttcttggac agcgaagaca gcaagctgta
    10741 aaccaaaaaa ccgcaggttt caaatacctg cggttttttc ttacacaata aacaacgctt
    10801 ccacatatcc cacactccta tcc
//
LOCUS       DAXHSF010000052        10810 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.052, whole genome shotgun sequence.
ACCESSION   DAXHSF010000052 DAXHSF010000000
VERSION     DAXHSF010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10810
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.052"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(121..1338)
                     /locus_tag="ACFM8S_001011"
     CDS             complement(121..1338)
                     /locus_tag="ACFM8S_001011"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFC6314095.1"
                     /translation="MALIVHKYGGTSVGSPERIKNVAKRVAKARAEGHDIVVVVSAMS
                     GETNRLVALAHEMQEHPDPRELDVVLSTGEQVTIGLLAMALKDIGVDAKSYTGWQVAL
                     KTDTAHTKARIESIDDEKMRADLDAGKVVIVAGFQGISSEGDISTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEARRMDTVTFEEMIELASLGSKVLQIRSVEF
                     AGKYKVRLRVLSSLQDGGNGTLITFEEDDNMERAAVTGIAFDKNQARINVRGVPDKPG
                     VAYQILGAVADANIEVDMIIQNVGSEGTTDFSFTVPRGDYKQTLEILSERQDSIGAVS
                     IDGDDTVCKVSAVGLGMRSHVGVAAKIFRTLAEEGINIQMISTSEIKVSVLIDEKYME
                     LATRVLHKAFNLG"
     gene            complement(1540..2268)
                     /gene="rph"
                     /locus_tag="ACFM8S_001012"
     CDS             complement(1540..2268)
                     /gene="rph"
                     /locus_tag="ACFM8S_001012"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFC6314096.1"
                     /translation="MPDYIRISRAADSLRDIKITPHFLPHADGSCLIECGNTKVICTA
                     SIDENVPPFLRGKNQGWVTAEYGMLPASTASRMRREASAGKQSGRTQEIQRLIGRSLR
                     AVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPIRE
                     AVAAVSVGVVNGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEDAPFSLDELG
                     KLVALAQKGIGELLQHQQNALSAA"
     gene            complement(2350..2814)
                     /locus_tag="ACFM8S_001013"
     CDS             complement(2350..2814)
                     /locus_tag="ACFM8S_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFC6314097.1"
                     /translation="MYKHLVVAVDGSETSINALKHAAELAGVNSARLTLVHVANPAEY
                     MALAPEFLQHESYEAAAVAQGNEVLDAAERTARELGVENTVKHLLVANKGAREMAQDL
                     VDYADENGADLLVLGTHGRTGLMHLLMGSFAETVMRQSHLPLLIIRSKAEEA"
     gene            3031..3807
                     /locus_tag="ACFM8S_001014"
     CDS             3031..3807
                     /locus_tag="ACFM8S_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HFC6314098.1"
                     /translation="MSDNALTSSRRFGGIARLYGDSALAHFSQAHVCVVGVGGVGSWA
                     IEALARTGIGRLTLIDLDNVAESNVNRQLHALTGDFGKAKVTALRERVAQINPGCQVF
                     EIEDFVTEDNLPEYFGKGFDFVIDAIDQVRVKAAMAAYFVERKQPFVLSGGAGGQKNP
                     ALIQTADLSRVTHDPLLANLRYTLRKRYGFSRDTKAKMRVPCVFSTENITPPQSGAAC
                     STDAAPQGLSCAGYGASMLVTASFGLYCAQAAVEHIADKK"
     gene            3945..4472
                     /locus_tag="ACFM8S_001015"
     CDS             3945..4472
                     /locus_tag="ACFM8S_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314099.1"
                     /translation="MEFVNNLVIFSFLLLMLIPIFFVVYGIYHKIRYRKIYILRISFT
                     LLVVILCSMYYIYCRYLDQQKVAYYCIDEQCISIVHLYKDYGINSPTYARIYAGKILF
                     RFQVRAKNYAELLMEDDISISKKILGNKFIIYGSLPVIYGNVDNIEVKEATGYIDRAS
                     TDYIVSRNLKFRHLY"
     gene            4643..5497
                     /locus_tag="ACFM8S_001016"
     CDS             4643..5497
                     /locus_tag="ACFM8S_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HFC6314100.1"
                     /translation="MPPIPAPSAPSEHTVAWVFGQPVTDLPQDLFIPPDALKVVLGSF
                     QGPLDLLLYLIRKQNIDVLDIPMVKITEQYLHYIAQIETYQFDLAAEYLLMAAMLIEI
                     KSRLLLPRTETVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAYLPLE
                     IAVEAKLPEVYITDLTQAWLGILSRAKHTRSHEVIKETISVRAQMTAILRRLNKHGIC
                     RFHDLFNPEQGAAYVVVNFISLLELAKEGLVRIVQEVGFGEIRISLNHEGAHSDGISG
                     TRGGRDVF"
     gene            5538..6746
                     /gene="pncB"
                     /locus_tag="ACFM8S_001017"
     CDS             5538..6746
                     /gene="pncB"
                     /locus_tag="ACFM8S_001017"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFC6314101.1"
                     /translation="MTGIIHSLLDTDLYKFTMLQVVLHQFPQTHSLYEFRCRNASTVY
                     PLADIKEDLEVELDALCQLRFTHDELGYLRSLRFIKSDFVDYLELFQLQRRFVEIGTD
                     DKDRLNIRIEGPMIQAMFFEIFILAIVNELYFRRLETPAVIEEGERRLQAKATRLKEI
                     AAAQNPDEPPFLISDFGTRRRYKLAWQEHVIRTLLEAAPSIVRGTSNVFLAKKLGITP
                     IGTMAHEFLQAFQALDVRLRNFQKAALESWVHEYRGDLGVALTDVVGMDAFLRDFDLY
                     FAKLFDGLRHDSGDPYIWGDKAYAHYQKLKIDSRTKMLTFSDGLDIERSWALHQYFKD
                     RFKTGFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNSTFLAYLRQ
                     VFDVPEPETP"
     gene            6838..8556
                     /gene="argS"
                     /locus_tag="ACFM8S_001018"
     CDS             6838..8556
                     /gene="argS"
                     /locus_tag="ACFM8S_001018"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFC6314102.1"
                     /translation="MNLHQTVEREAAAAFAAAGIAGSPVVLQPTKNAEHGDFQINGVM
                     GAAKKAKQNPRELAQKVAEALAGNAVIESAEVAGPGFINLRLRPEFLAQNIQTALNDA
                     RFGVAKTDKPQTVVIDYSSPNLAKEMHVGHLRSSIIGDSISRVSEFMGNTVIRQNHVG
                     DWGTQFGMLVAYLVEQQKDNAAFELADLEQFYRAAKVRFDEDPAFADTAREYVVKLQG
                     GDETVLALWKQFVDISLSHAQAVYDTLGLKLRPEDVAGESKYNDDLQPVVDDLVQKGL
                     AVEDDGAKVVFLDEFKNKEGEPAAFIVQKQGGGFLYASTDLACLRYRVGTLHADRLLY
                     VVDHRQALHFEQLFTTSRKAGYLPENVGAAFVGFGTMMGKDGKPFKTRSGDTVKLVDL
                     LTEAVERATALVKEKNPELGADEAAKIGKTVGIGSIKYADLSKNRTSDYVFDWDAMLS
                     FEGNTAPYLQYAYTRVQSVFRKAGEWDANAPTVLTEPLEKQLAAELLKFEDVLQSVAD
                     TAYPHYLAAYLYQIATLFSRFYEACPILKAEGASRNSRLQLAKLTGDTLKQGLDLLGI
                     DVLDVM"
     gene            complement(8492..8893)
                     /locus_tag="ACFM8S_001019"
     CDS             complement(8492..8893)
                     /locus_tag="ACFM8S_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314103.1"
                     /translation="MTDRCRPGAKVWHGVAGGAHACRGKENREKRRAETLPEEKAGLR
                     GGKRGMRRAESACVGRKGVIIFCLLACLLAWRLFVSHFGRGGGYGVSRRFFQIRIRMA
                     RLLSAIGRCGFTLRPTHRCPANPSLVSACRR"
     gene            9088..10506
                     /locus_tag="ACFM8S_001020"
     CDS             9088..10506
                     /locus_tag="ACFM8S_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFC6314104.1"
                     /translation="MNIHTLLSKQWTLPPFLPKRLLLSLLILLAPNAMFWVLALLTAT
                     ARPIVNLDYLPAALLIALPWRFVKITGVLAFWLAVLFDGLMMVIQLFPFMDLIGAINL
                     VPFILTAPAPYQIMTGLLLLYMLAMPFVLQKAAAKTDFRHIAVCAAVVVAVGYFTGHL
                     SYYDRGRMANIFGANNFYYAKSQAMLYTVSQNADFITAGLVDPVFLPLGNQQRAATHL
                     NEPKSQKILFIVAESWGLPANPELQNATFAKLLAQKDRFSVWESGSFPFIGATVEGEM
                     RELCAYGGLRGFALRRAPDEKFARCLPNRLKQEGYATFAMHGAGSSLYDRFSWYPRAG
                     FQEIKTAENLIGKKTCAIFGGVCDSELFGEVSAFFKKHSKGLFYWMTLTSHADYPESD
                     IFNHRLKCTEYGLPAETDLCRNFSLHTQFFDQLADLIQRPEMKGTEVIIVGDHPPPVG
                     NLNETFRYLKQGHVAWLHFKIK"
BASE COUNT         2581 a         3065 c         2649 g         2515 t
ORIGIN      
        1 atttaatcca ctatattttt tgttccaaag tcaaaatatg ccgtccgaac attcgggcgg
       61 cagacaaaac ggcactgccc gataaaggca gtgccgttgt ccgtttcaaa ccgtgaaaca
      121 tcagcccaaa ttaaaggctt tatgcaatac cctggttgcc agttccatgt atttttcatc
      181 aatcaatacg gaaactttga tttcggaggt ggaaatcatt tggatgttga taccctcttc
      241 ggcgagcgtg cggaagattt tggcggctac accgacgtgc gaacgcatac ccaaaccgac
      301 tgcagagact ttgcatacgg tgtcgtcgcc atcaatagaa accgcgccga tactgtcttg
      361 gcgttccgac aggatttcca aagtctgctt gtaatcgccg cgcggtacgg taaaggaaaa
      421 atcggttgtg ccttcgctgc cgacattttg gataatcata tcgacttcga tgttggcatc
      481 ggcaaccgcg cctaaaatct gataggcgac accgggtttg tcgggtacgc cgcgcacgtt
      541 gatgcgggct tggtttttat cgaatgcgat accggttacg gcagctcttt ccatgttgtc
      601 gtcctcttca aaggtaatta aggtgccatt gccgccgtct tgcaggctgc tcagtacgcg
      661 caggcgcact ttgtattttc cggcgaattc tactgaacgg atttgcaaaa ctttcgaacc
      721 gaggcttgcc agttcgatca tttcttcaaa tgtaaccgta tccatgcggc gcgcttcggg
      781 tacgacgcgg gggtcggttg tgtaaacgcc gtctacgtcg gtatagattt ggcactcgtc
      841 ggctttgagg gctgcggcaa gcgcaacggc ggaagtgtcg gaaccgccgc gtccgagcgt
      901 ggaaatatcg ccttcactgc tgatgccttg gaagccggca acgatgacga ctttgcctgc
      961 atcgaggtcg gcacgcattt tttcgtcatc aatgctttcg atgcgggctt tggtgtgggc
     1021 ggtatcggtt ttgagggcga cctgccagcc tgtgtagctt ttggcatcca cgccgatgtc
     1081 tttcaatgcc atcgccaaaa ggccgatggt tacttgttcg ccggtggaca aaaccacgtc
     1141 caattcgcgc ggatcgggat gctcctgcat ttcgtgcgcc aacgcgacca ggcggttggt
     1201 ttcaccgctc atggcggata cgacgacgac gatgtcgtgt ccttcggcgc gggctttggc
     1261 gacacgtttg gccacgtttt taatgcgttc gggcgagcct acggatgtgc cgccgtattt
     1321 gtgtacgatt aacgccatgt ttcgtgcttt cttgtgtggg attggtcggg cagcatggtt
     1381 tgctggaaaa gcggctatta ttactatttt ttacatgaag ttcaagaacg gactgcgctt
     1441 tcccacctgc cgtttgacag cggtcagcaa aaaacctgtt ctttcagatt gttgacaaaa
     1501 tgccgtctga acggttttca gacggcatcc ggacgacaat caggcggcag acagcgcatt
     1561 ttgctggtgt tgcagcagtt cgcctatgcc tttttgcgcc agtgcaacca gtttgcccaa
     1621 ttcgtccaaa ctgaacggcg cgtcttccgc cgtaccttgg atttcgatga tttttcccga
     1681 tgcggtcatg acgatattca catcactgtc gcaaccggag tcttcgggat aatccaaatc
     1741 caaaagcggc acgccgttca ctacgcctac tgacacagcg gcaacggctt cgcggatggg
     1801 gttttcactc aaaatgccgt ctgaaaccag tttgccgacg gcgatttgca gcgcgacaaa
     1861 cgcaccggta atcgaagccg tgcgcgtacc gccatctgcc tgaatcacat cgcagtcaat
     1921 caagatttgt cgttcgccga gtttttccat atccacgacc gcgcgtagcg aacgcccgat
     1981 aaggcgttgg atttcttgtg tgcgcccgga ctgtttgccc gccgaagctt cgcggcgcat
     2041 acgcgaagcg gtggatgcag gcagcatccc gtattctgcg gttacccagc cttggttttt
     2101 gccgcgcaaa aacggtggga cattttcgtc tatggaagcg gtacaaatca ctttggtatt
     2161 accgcattca ataaggcacg aaccgtcggc gtgcggcagg aaatgagggg tgattttgat
     2221 atcgcgcagg ctgtcggcgg cgcgcgagat gcggatgtaa tcaggcatac tgccctcccg
     2281 ttaaaaacag ataaattaaa aagccttaaa tatgaaaaat cacatttaag gccttcaaac
     2341 tgaaaatttc tacgcctctt cggctttgct gcggataatc aaaagcggca ggtggctttg
     2401 gcgcattacc gtttcggcaa aactgcccat taaaaggtgc atcagcccgg tacgtccgtg
     2461 cgtacccaac accagcaagt cggcaccgtt ttcatcggca taatcaacca aatcctgcgc
     2521 catttcacgc gcacccttat tggcaaccag caggtgtttg acggtatttt ccacacccag
     2581 ttcccgggcg gtgcgctcgg cggcatccaa aacttcgttg ccttgcgcga cggcggcggc
     2641 ttcgtagctt tcgtgttgca aaaattcggg ggcgagcgcc atatattcgg caggattggc
     2701 aacgtgcacc aaagtcaggc gcgcgctgtt gactcctgca agctcggcgg catgtttcag
     2761 ggcattgatg gacgtttcac tgccgtcaac ggcaacgacc agatgtttgt acatatcgta
     2821 ttctcctttt gcaccgcctc gcggtgccct cttgtcggat gggcgcaggg acagtttgcg
     2881 ccgtttcatt atagacccgc cgtcgggctt tatacaacag ccgaacagcc cgaccgcttt
     2941 ccagtataat atgccgcttc cgtgcagtca ggcatttttt gccggctttc gttcactttt
     3001 tgatttgacg caatcttgca ggattcgacc atgtccgaca acgctttgac ctcttcgcga
     3061 cgcttcggcg gcatcgccag actctacgga gactctgcct tggcgcactt ttcacaggca
     3121 cacgtctgcg tagtcggcgt gggcggtgtc ggctcgtggg cgatcgaggc tttggcgcgg
     3181 acgggcatcg gacgtttgac tttgattgat ttggacaacg ttgccgaatc gaatgtcaac
     3241 cgccagctgc acgccctgac cggagacttc ggcaaagcaa aagttaccgc cttgcgcgaa
     3301 cgcgtcgccc aaatcaaccc aggatgccaa gtgtttgaaa ttgaagattt cgttaccgaa
     3361 gacaatttgc cggaatactt cggaaaaggt tttgatttcg tcatcgacgc gatcgaccaa
     3421 gtgcgcgtca aagcagcaat ggcggcttat tttgtggaac gcaaacaacc gtttgtcctc
     3481 agcggcggcg cgggcggaca aaaaaatccg gcgttaatcc aaaccgccga tttgagccgc
     3541 gtaacccacg atccgctgct tgccaacctg cgctacacct tgcggaaacg ctacggcttc
     3601 agccgcgata cgaaagccaa aatgcgcgtg ccgtgcgtgt tctcaaccga aaacatcacg
     3661 ccgccgcaat cgggcgcggc gtgttctacc gatgccgctc cgcaaggctt gtcgtgcgcc
     3721 ggctacggtg caagcatgct cgttaccgct tcgttcgggc tatattgcgc acaggcggcg
     3781 gtggaacaca tcgcagacaa aaaataagca atgccgtctg aaacaggatt cagacggcat
     3841 ttgaacatac tatggttatg atttaggaca acaaaggata cggataagaa ataacataaa
     3901 atatatgatt cctaataata taccaagtat cggagagcta tttaatggaa ttcgttaata
     3961 atttagttat tttttcattt ttattactaa tgcttattcc gatatttttt gtagtatatg
     4021 gtatatacca taagatacgt tatcgcaaaa tatatatcct aagaataagt tttacattat
     4081 tagtggtaat actttgcagt atgtattaca tatattgccg ttatcttgac caacaaaaag
     4141 tagcttatta ttgcatagat gaacaatgta tttctattgt tcatctatac aaagattatg
     4201 gtataaactc tcccacatat gcgagaattt acgcaggaaa aatattgttt agatttcaag
     4261 taagagctaa aaattacgct gaattactta tggaagatga tatatcaatt agtaaaaaaa
     4321 ttttggggaa taaatttatc atttatgggt cgctacctgt aatatacggt aatgtagata
     4381 atattgaagt aaaagaagct actggttata tagatagagc cagtactgat tatattgtct
     4441 caagaaactt aaaattcaga catttatatt aattaagagg ttttagcaag accgtattca
     4501 caacctgctc cttttcaaaa catttgcatt taaaagccgt tataatgccg tctgaacatc
     4561 tgcccgacca cattatgcgt gaatgtcggc agattgtttt cttttgtaaa cttatattaa
     4621 aatccactta ccgattcacg ccatgccgcc catccctgcc ccatccgcac catccgagca
     4681 cactgtcgca tgggtattcg gccaacccgt taccgatttg ccgcaggatt tgtttatccc
     4741 gcccgacgcg ctgaaagtcg tattgggcag ttttcaaggc cctttggatc tactgctgta
     4801 tctgatccgc aagcagaaca tcgatgttct cgatattccg atggtgaaga ttaccgagca
     4861 gtatctgcac tacatcgccc aaatagaaac ctatcagttt gatttggcgg cggaatatct
     4921 tttgatggca gcaatgctga ttgaaatcaa atcgcgcctg ctgctgccgc gtaccgaaac
     4981 cgtcgaagac gaagaagccg acccgcgtgc cgagttggtg cgccgcctgc tggcttacga
     5041 acagatgaag ctggcggcgc agggtttgga cgcgctgccc cgagccggac gggatttcgc
     5101 gtgggcttac ctgccgctgg aaattgccgt cgaagccaag ctgcccgaag tctatattac
     5161 cgacttgacg caagcgtggc tgggtatttt gtctcgggca aaacatacgc gcagccacga
     5221 agttatcaaa gaaaccatct ccgtgcgcgc gcaaatgacg gcaatcctgc gccgtttgaa
     5281 caaacacggg atatgcaggt ttcacgacct gttcaatccc gaacagggcg cggcttacgt
     5341 ggtcgtcaac ttcatctcac tgttggagct tgccaaagaa ggattggtca gaatcgtgca
     5401 ggaagtcggt ttcggagaaa ttcgaatcag cctcaatcat gagggggcgc attcagacgg
     5461 catttccggc acacggggcg ggcgcgatgt gttctaatac gccccaagcc gccaccaaaa
     5521 atcgggagac acgccatatg accggcatca tacattcgct gcttgacacc gacctctaca
     5581 aattcactat gctgcaagtg gttctgcacc agtttccgca gacgcacagc ctttacgaat
     5641 tccgctgccg caacgcctcg accgtctatc cgcttgccga catcaaagaa gacttggaag
     5701 tcgaactcga cgcgctctgc caactgcgct ttacccatga cgaactcggc tacctccgca
     5761 gcctgcgttt catcaaaagc gactttgtcg attatctcga actcttccag ctccaacgcc
     5821 gctttgtcga aatcggcaca gacgataaag accgtctgaa catccgcatc gaaggtccga
     5881 tgatacaggc gatgtttttt gaaatcttca tcctcgccat tgtcaacgaa ctttacttcc
     5941 gccggctgga aacccccgcc gttatcgaag aaggcgaacg ccggcttcaa gccaaagcca
     6001 cgcgcctgaa agaaatcgcc gccgcacaaa accccgacga accgcccttc ctgatttccg
     6061 acttcggcac gcgccgccgc tacaagctcg cgtggcagga acacgtcatc cgcaccctgc
     6121 tcgaagccgc cccaagcatc gtgcgcggca ccagcaatgt ctttctcgcc aaaaaactcg
     6181 gcatcacccc catcggcacc atggcgcacg aattcctcca agccttccaa gccctcgacg
     6241 tgcgcctgcg gaatttccaa aaggccgcgc tcgaaagctg ggtacacgaa taccggggcg
     6301 atttgggtgt cgccctgacc gacgtggtcg gcatggatgc cttcctgcgc gatttcgacc
     6361 tctatttcgc caaactcttc gacgggctac gccacgacag cggcgaccct tacatttggg
     6421 gcgacaaagc ctacgcccac tatcaaaagc tcaaaatcga cagccgcacc aaaatgctga
     6481 ccttctccga cgggctggac atcgaacgct cttgggcatt acaccaatat ttcaaagacc
     6541 gcttcaaaac cggcttcggc atcggcacca acctcaccaa cgatatgggg catacgccct
     6601 tgaatatcgt cttaaaactg gtcgaatgca acgggcagtc cgtcgccaag ctgtccgact
     6661 ctccgggcaa aaccatgacc aacaacagca ccttcctcgc ctacctgcgc caagtgttcg
     6721 acgtacccga acccgaaacg ccgtaaaccg gcagaaaaag cgcacaattc ctgtttctgc
     6781 cgcataaaat cttttaaaat accgcctgat ttgaatttaa ccgaaagacc gaacttcatg
     6841 aacctacatc aaaccgtcga acgcgaagcc gccgccgcct ttgccgccgc aggcatcgcc
     6901 ggcagccccg ttgtcttgca gccgaccaaa aatgccgaac acggcgattt ccaaatcaac
     6961 ggcgtgatgg gtgcggcaaa aaaagccaaa caaaacccgc gcgaattggc gcaaaaagtt
     7021 gccgaagcat tggcgggcaa cgccgtcatc gaaagcgcgg aagtcgccgg cccgggcttt
     7081 atcaacctgc gcctgcgccc cgaatttctc gcgcaaaaca ttcagacggc cttgaacgac
     7141 gcgcgtttcg gcgtggcaaa aaccgacaaa ccgcaaaccg tcgttatcga ctattcctcg
     7201 cccaatctgg caaaagaaat gcacgtcggc catctgcgtt ccagcatcat cggcgacagc
     7261 atttcgcgcg tgtcagaatt tatgggcaat accgttatcc gtcaaaacca cgtcggcgac
     7321 tggggtacgc agttcggcat gttggtcgct tatttggtcg agcagcaaaa agacaatgcc
     7381 gcgttcgagc tggcggattt ggagcagttt taccgcgccg ccaaagtgcg ctttgacgaa
     7441 gatcctgcct tcgccgacac cgcgcgcgaa tacgttgtga agctgcaagg cggcgatgaa
     7501 accgttttgg cattgtggaa acagtttgtc gatatttcgc tctcgcacgc ccaagccgtt
     7561 tacgacacgc tgggcttgaa gctgcgcccc gaagacgtgg cgggcgaatc gaaatacaac
     7621 gacgatttgc agcccgtagt cgatgatttg gttcaaaaag gtctggcagt cgaagacgac
     7681 ggcgcaaaag tcgtgttctt ggacgagttt aaaaacaaag agggcgaacc tgccgcattt
     7741 atcgtgcaaa aacaaggcgg cggcttcctt tatgcttcca ccgacttggc gtgtttacgc
     7801 taccgtgtcg gcacgcttca tgccgaccgc ctgctgtacg tcgtcgacca ccgccaagcc
     7861 ctgcactttg agcaactctt taccacttca cgcaaagccg gctatctgcc ggaaaacgtc
     7921 ggcgcggcat ttgtcggctt cggcacgatg atgggcaaag acggcaaacc gttcaaaaca
     7981 cgcagcggcg acaccgtgaa actggtcgat ctgctgaccg aagccgtcga acgcgccacc
     8041 gctttggtga aagaaaaaaa tcccgaattg ggtgcggacg aagccgctaa aatcggcaaa
     8101 accgtcggca tcggctcaat caaatacgcc gatttgagca aaaaccgcac cagcgattat
     8161 gtgttcgact gggacgccat gctctcgttt gaaggcaaca ccgcccccta tctgcaatac
     8221 gcctacaccc gcgtgcaaag cgtgttccgc aaagcaggcg aatgggatgc aaatgcgcca
     8281 accgttttga ccgaaccgct ggaaaaacag cttgccgccg agctgctgaa atttgaagac
     8341 gtactgcaaa gcgtggcgga cacggcgtat ccgcactacc tcgccgccta cctctatcaa
     8401 attgcgaccc tgttcagccg cttctacgaa gcctgtccga tactcaaagc cgaaggcgca
     8461 agccgcaaca gccgcctgca actggcaaaa ctcaccggcg acacgctgaa acaaggcttg
     8521 gatttgctgg gcatcgatgt gttggacgta atgtaaaacc gcaccgcccg attgcggaca
     8581 acagcctcgc catccttatc cgaatctgaa aaaagcggcg cgatacaccg tatccgccgc
     8641 ccctcccaaa atgcgaaaca aacaaacgcc aagcaagcaa gcaagcaagc aagcaaaaaa
     8701 ttataacccc cttcctgccg acgcacgcac tttccgcgcg gcgcattccc cttttcccgc
     8761 ccctcaaacc cgccttttct tcaggcaggg tttcagcccg cctcttttcc ctgttttcct
     8821 ttccccgaca cgcgtgcgca ccccctgcca caccgtgcca cactttcgcg cccggacggc
     8881 atcggtctgt catccggttc tctgttttaa atacccctgt ttcagaaaga aatgcagatg
     8941 tttcaacaca caggacgaca tataaagcac cgccctatgt gttgccctga tttgaaaggg
     9001 gttgcgctcc tcccaaataa agtctgatcc tgccgccccg aaggacagat gtccgagtgg
     9061 cgaagtttca accgaaaagg aaatacgatg aatattcaca ccctgctctc caaacaatgg
     9121 acgctgccgc catttctgcc gaaacggctg ctgctgtccc tgctgatact gctggctccc
     9181 aatgcgatgt tttgggtttt ggcactgctg accgccaccg cccgcccgat tgtcaatttg
     9241 gactaccttc ccgccgcact gctgatcgcc ctgccttggc gtttcgtcaa aattaccggc
     9301 gtattggcgt tttggctggc ggttttgttt gacgggctga tgatggtgat ccagcttttc
     9361 ccttttatgg acctcatcgg cgccatcaac ctcgtcccct tcatcctgac cgcccccgcc
     9421 ccttatcaga taatgaccgg gctgttgctg ctgtatatgc tggcgatgcc gtttgtgttg
     9481 cagaaagccg ccgccaaaac cgacttccga cacattgccg tctgcgccgc cgttgtggtg
     9541 gcagtcggct atttcaccgg ccatttgagt tactacgacc gggggcggat ggccaatatc
     9601 ttcggcgcaa acaacttcta ttacgccaaa agtcaggcga tgctctacac cgtcagccag
     9661 aatgccgact ttattaccgc cggcctggtc gatcccgtct tcctcccctt gggcaatcag
     9721 cagcgtgccg ccacgcatct gaacgagccg aaatctcaaa aaatcctctt tatcgtcgcc
     9781 gaatcttggg ggctgccggc caatcccgaa cttcaaaacg ccacttttgc caaactgctg
     9841 gcgcaaaaag accgtttttc agtttgggaa agcggcagtt ttccctttat cggcgcgacg
     9901 gtcgaaggcg aaatgcgcga actgtgcgcc tacggcggtt tgcgcgggtt cgcactgcgc
     9961 cgcgcgcccg acgaaaaatt tgcccgctgc ctccccaacc gtttgaaaca agaaggttac
    10021 gccacctttg cgatgcacgg cgcgggcagt tcgctttatg accgcttcag ctggtatccg
    10081 agggcgggct ttcaagaaat caaaaccgcc gaaaacctga tcggtaaaaa aacctgcgcc
    10141 attttcggcg gcgtgtgcga cagcgagctg ttcggcgaag tgtcggcatt tttcaaaaaa
    10201 cacagcaaag gattgtttta ctggatgacg ctgaccagcc acgccgacta tcccgaatcc
    10261 gacattttca accacaggct caaatgcacc gaatacggcc tgcccgccga aaccgacctc
    10321 tgccgcaact tcagcctgca cacccaattc ttcgaccaac tggcggattt gatccaacgc
    10381 cccgaaatga aaggcacgga agtcatcatc gtcggcgacc atccgccgcc cgtcggcaac
    10441 ctcaatgaaa ccttccgcta cctcaaacag gggcacgtcg cctggctgca cttcaaaatc
    10501 aaataacaac aatgccgtct gaacgcacca acagccttca gacggcattt tgcagacaga
    10561 ccgacccttc aagcccactt ttttcatcat ctccgataaa ttgctttgta tagtggatta
    10621 acaaaaacca gtacggcgtt gcctcgcctt agctcaaaga gaacgattct ctaaggtact
    10681 gaagcaccaa gtgaatcggt tccgtactat ttgtactgtc tgcggcttcg tcgccttgtc
    10741 ctgatttttg ttaatccgcc ataaagaccg tcgggcatct gcagccgtca ttcccgcgca
    10801 ggcgggaatc
//
LOCUS       DAXHSF010000053        10769 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.053, whole genome shotgun sequence.
ACCESSION   DAXHSF010000053 DAXHSF010000000
VERSION     DAXHSF010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10769
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.053"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            79..1248
                     /gene="metZ"
                     /locus_tag="ACFM8S_001021"
     CDS             79..1248
                     /gene="metZ"
                     /locus_tag="ACFM8S_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HFC6314105.1"
                     /translation="MSKKLHPQTLAIRGGKEQTEYREHNQALFLTSSFMWDNAQHAAD
                     LFSKKIKGFTYTRTANPTTAAFEKRIAALEGAERAVATSTGMSAIQAAFFTFLQAGDH
                     VISSRSLFGTTVGFINNIVTKFGIEVSHVSPTDINEWKAAVKANTKLLFLETPSNPLG
                     EVADLEALAELAHGIGALLVVDNSLLSPVGSQPLKHGADISVSSATKAIDGHGRVMGG
                     VLAGSEELMAQVAVYCNSCGLAMSPFNAWQLLSGVETLSLRMEKQFDNALKIAQWLQA
                     QPQVQAVYYTGLPDHPQAELIRKQQNGGGIVIGFEVADQAAAWKVVDGVELFSRTANL
                     GDVRSTITHPWTTTHGRMQPEEKLAANIRPGLVRLSVGLEYVGDLIDDLKQALAR"
     gene            1343..1549
                     /locus_tag="ACFM8S_001022"
     CDS             1343..1549
                     /locus_tag="ACFM8S_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HFC6314106.1"
                     /translation="MDEAVFADWALKICLTGLIIFLGFIVWNLGKESKAGKFGIAILF
                     LVLGLGVFGFVFKELLIKFLVLPK"
     gene            complement(2393..3145)
                     /locus_tag="ACFM8S_001023"
     CDS             complement(2393..3145)
                     /locus_tag="ACFM8S_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6314107.1"
                     /translation="MTDILIDNTATETVRTLIRAFPLVPVSQPPEQGSYLLAEHDTVS
                     LRLVGEKSSVIVDFASGAAQYRRTKGGGELIAKAVNHTAKPTVWDATAGLGRDSFVLA
                     SLGLTVTAFEQHPAVACLLSDGIRRALLNPETQDTAARITLHFGNSAVQMPALVQTQG
                     KPDIVYLDPMYPERRKSAAVKKEMTYFHRLVGEAQDEAVLLHTARQTAKKRVVVKRPR
                     LGEHLAGQAPAYQYTGKSTRFDVYLPYEADKG"
     gene            complement(3249..3716)
                     /locus_tag="ACFM8S_001024"
     CDS             complement(3249..3716)
                     /locus_tag="ACFM8S_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004518841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFC6314108.1"
                     /translation="MTELQHPVLVVDDETDILDLMEMTLMKMGLRVHTASGVAEAKNK
                     LDSQRYSLVLTDMRMPDGSGLEVVQHINSRLLDTPVAVITAFGNADQAQEALRCGAFD
                     PDTMQIQDYLDQIERDIIEQTLKQTEGNRTQAAKRLGISFRSMRYRMERLNIG"
     gene            complement(3709..4887)
                     /locus_tag="ACFM8S_001025"
                     /pseudo
     CDS             complement(3709..4887)
                     /locus_tag="ACFM8S_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            complement(4907..7210)
                     /gene="parC"
                     /locus_tag="ACFM8S_001026"
     CDS             complement(4907..7210)
                     /gene="parC"
                     /locus_tag="ACFM8S_001026"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFC6314109.1"
                     /translation="MNTQAHAPHTDSNTLMLGRYAERAYLEYAMSVVKGRALPEVSDG
                     QKPVQRRILFAMRDMGLTAGAKPVKSARVVGEILGKYHPHGDSSAYEAMVRMAQDFTL
                     RYPLIDGIGNFGSRDGDGAAAMRYTEARLTPIAELLLSEINQGTVDFVPNYDGAFDEP
                     LHLPARLPMVLLNGASGIAVGMATEIPSHNLNEVTQAAIALLKKPTLETADLMQYIPA
                     PDFAGGGQIITPADELRRIYETGKGSVRVRARYEIEKLARGQWRVIVTELPPNANSAK
                     ILAEIEEQTNPKPKAGKKQLNQDQLNTKKLMLDLIDRVRDESDGEHPVRLVFEPKSSR
                     IDTDTFINTLMAQTSLEGNVSMNLVMMGLDNRPAQKNLKTILQEWLDFRTVTVTRRLK
                     FRLNQVEKRLHILEGRLKVFLHIDEVIKVIRESDDPKADLMAAFGLTEIQAEDILEIR
                     LRQLARLEGFKLEKELNELREEQGRLNILLSDENEKRKLIVKEMQADMKQYGDARRTL
                     VEEAGRAVLTQTTADEPITLILSEKGWIRSRAGHNLDLSQTAFKEGDCLKQTLEGRTV
                     LPVVILDSSGRTYTLDAAEIPGGRGDGVPVSSLIELQNGAKPVAMLTGLPEQHYLLSS
                     SSGYGFITKLGDMVGRVKAGKVVMTADSGETVLPPVAVYASSFINPDCKIIAATSQNR
                     ALAFPIGELKIMAKGKGLQIIGLNAGESMTHTAVSSELEILIESEGRRGAAHKDRIPI
                     SLLEAKRGKKGRLLPISGSLKQLSSPK"
     gene            7358..8041
                     /locus_tag="ACFM8S_001027"
     CDS             7358..8041
                     /locus_tag="ACFM8S_001027"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFC6314110.1"
                     /translation="MELVFIRHGQSEWNAKNLFTGWRDVKLSEQGLAEAAAAGKKLKE
                     NGYEFDIAFTSVLTRAIKTCNIVLEESDQLFVPQIKTWRLNERHYGQLQGLDKKQTAE
                     KYGDEQVRIWRRSYDTLPPLLDKDDEFSAHKDRRYAHLPADVVPDGENLKVTLERVLP
                     FWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDEDIMGLEIPTGQPLVYKLDDN
                     LKVIEKFYL"
     gene            complement(8832..>10769)
                     /locus_tag="ACFM8S_001028"
     CDS             complement(8832..>10769)
                     /locus_tag="ACFM8S_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HFC6314111.1"
                     /translation="ISPEIFGTLFQSVMDAQERREAGAHYTETANIDKVINGLFLENL
                     RAEFEAVKALKRDKDKKLAAFYQKIQNLQFLDPACGCGNFLIVAYDRIRALEDDIIAE
                     ALKDKTGGLFDSPSVQCRLKQFHGIEIDEFAVLIARTAMWLKNHQCNIRTQIRFDGEV
                     ACHTLPLEDAAEIIHANSLRTPWQAADYIFGDPPFIGSTYQTKEQKNDLESICGHIKG
                     YGLLDYVCNWYVKAAGIMAQHPQVQTAFVSTNSICQGQQVEILWGSLLKQDIEIHFAH
                     RTFQWTSQAAGKAAVHCIIVGFRQKPQMPSEKTLYDYPDIKGEPVKHAAANINPYLID
                     APDVIIAKRSRPVHCEPDMVNGSKPTEGGNLILSTDEKEALIAAEPLAEQYIRPFVGA
                     EEFINGKTRWCLWFHGVSDVKRNHDLKQMPQVQARIQAVKTMREASSDKQTQKDAATP
                     WLFQKIRQPSDGNFLIIPRVSSESRRFVPIGYLSYETVVSDLVFTLPNASLYHFGILS
                     STMHNAFMRTVAGRLESRYRYSNTVVYNNFPFPESCRMPSENDRQDPLRAAVETAAQA
                     VLDARGQYRREAREAGLPEPTLAELYAPDAGYTALDKAHAALDKAVDKAYGYKTGKNT
                     DDEAERTAFLFELYRKAAAIA"
BASE COUNT         2260 a         2676 c         3214 g         2619 t
ORIGIN      
        1 ggtttaaatt taatccacta taaattaaac aacgggcgca cgctccgttg ccgaacggat
       61 aaaaccgagg aatcaccgat gagcaaaaaa ctccatccgc aaacgctcgc catacgcggc
      121 ggcaaagaac aaaccgaata ccgcgaacac aatcaggcat tgttcctgac cagcagcttc
      181 atgtgggaca acgcccaaca cgctgccgat ttgttttcaa aaaaaatcaa aggcttcact
      241 tatacccgta ccgccaaccc gaccacagcc gcctttgaaa aacgcatcgc cgctttggaa
      301 ggtgcggaac gcgcggtcgc cacttcgacc ggcatgtccg cgattcaggc ggcgtttttc
      361 acctttttgc aggcgggcga ccatgtgatt tccagccgca gcctgttcgg cacgaccgtc
      421 ggctttatca ataacatcgt taccaaattc ggcatcgaag tgagtcacgt gtcgccgact
      481 gatataaacg aatggaaagc cgccgtcaaa gccaacacca aactgctgtt tttggaaacg
      541 ccgtccaatc ccttgggaga agtggccgac ttggaagcct tggcggaatt ggcgcacggc
      601 atcggcgcgc tcttggtggt ggacaacagc ctgctgtcgc ctgtcggctc tcagcctttg
      661 aaacacggcg cggatatttc cgtttcctcc gcgaccaaag ccatagacgg acacgggcgc
      721 gtgatgggcg gcgtgttggc gggttcggaa gaactgatgg cgcaggtcgc cgtttactgc
      781 aactcttgcg ggctggcaat gtcgccgttt aacgcatggc agttgttgag cggcgtggaa
      841 accctgtctc tgcgtatgga aaaacagttc gacaacgcct tgaaaatcgc gcaatggctg
      901 caagcgcagc cgcaggttca agccgtgtat tacaccggat tacccgacca tcctcaggcg
      961 gagcttatcc gcaaacagca aaacggcggc ggcatcgtca tcggctttga agtcgccgac
     1021 caagcagccg cgtggaaagt cgtggacggc gtcgaacttt tctcccgtac cgccaacctc
     1081 ggcgacgtgc gctcgaccat tacccatccg tggacaacca cgcacggcag gatgcagccc
     1141 gaagaaaaac tcgccgccaa catccgcccc ggattggtgc gcctgtcggt cggtttggaa
     1201 tatgttggcg atttgattga cgatttgaaa caggcactcg cgcgttgatt gcgtgttcag
     1261 acggcatcgg cggaaaaaca ccgcaaatgc cgtctgaaac ccttatctta tatgacatac
     1321 acgtcaacaa taggaaaaaa atatggatga agcggttttt gccgattggg cgttgaaaat
     1381 ctgcctgacc ggcttgatta tttttttggg ttttatcgtt tggaatttgg gcaaggagtc
     1441 taaagcgggc aaattcggca tcgccatttt gtttttggtt ttgggcttgg gcgtgttcgg
     1501 ttttgtattt aaagaactgc taatcaagtt tttggtattg cccaaatagg ctgccgcctg
     1561 tcaaaatgct gtctgaaatc ataaaaagta atctgcaccc taaaagtttg atttaaccgc
     1621 caactgatta aggtgcggat ttttatgaca aaataaaaat catctttccg cagtcattcc
     1681 cacggatgcg aaagcctgtc tttttgaatc ccggataatt ttacatcgtc attcccgcgc
     1741 aggtgggaat ccagatcatt gggtagcggc aatgttcaaa agtcgtctga aaaatcagaa
     1801 gttctagatt cccgttttta cgggaatgac ggaatttcag acggcatccg cccgccccgt
     1861 cattcccgcg caggcgggaa tctagtccgt tcggtttcgg tcatttacga taaattcctg
     1921 ttgcttttca tttctggatt cccacttccg tgggaatgac ggaggagcgg ataaattctc
     1981 gcaatctaaa accttgtcat ttccataaaa aacagcaacc cgaaacagca acttaaaaac
     2041 agcaacctga aacctcgtca ttcccgcgca ggtgggaatc cagatcattg ggtggcggca
     2101 atgttcaaaa gtcgtctgaa aaatcagaag ttctagattc ccgttttcac gggaatgacg
     2161 gaatttcaga cggcatctgc ccgccccgtc attcccgcgc aggcgggaat ctagtccgtt
     2221 cggtttcagt catttacgat aaattcctgt tgcctttcat ttctagattc ccacttccgt
     2281 gggaatgaca aactttaagg tgtgatgact tatccaaaac agtcgaaacg caaaaaccgg
     2341 tttctcgttt gcataagaac ggcaaatttt tcggtgtctt gttttatggg cgttatccct
     2401 tgtccgcttc gtagggcagg taaacgtcga agcgggtgct tttgcctgtg tattggtatg
     2461 cgggggcttg tccggcaagg tgttctccca ggcgggggcg tttgacgacg acgcgttttt
     2521 ttgcagtctg tcgggcggta tggaggagga cggcctcatc ttgcgcctcg ccgacgagcc
     2581 ggtggaagta ggtcatttct tttttaacgg cggcactttt gcggcgttcg ggatacatgg
     2641 ggtcgagata gacgatgtcg ggtttgcctt gtgtttgaac aagggcgggc atttgtactg
     2701 cggagttgcc gaaatgcagg gtgatgcgcg cggcggtgtc ttgcgtttcg ggattgagga
     2761 gggcgcggcg gatgccgtct gaaagcaggc aggcgacggc gggatgttgc tcgaaggcgg
     2821 taacggtcag cccgagcgag gcgaggacga agctgtcgcg ccccaatcct gcggtcgcgt
     2881 cccaaacggt cggtttggcg gtatggttga cggctttggc gatgagttcg cccccgcctt
     2941 ttgtgcgccg gtattgtgcc gcgccggagg caaaatcgac gatgacgctg cttttttccc
     3001 cgacaagcct gaggctgacg gtatcgtgtt cggcaaggag gtaactgcct tgttcgggcg
     3061 gttgggaaac gggcacaagg gggaatgccc gtatcagggt gcggacggtt tcggtggcgg
     3121 tgttgtcaat aaggatgtcg gtcatggtgt tttgtgtccg ttcgggcgtg gggcgaaccc
     3181 gctttcccgt gccgtttcag acggcatttt ttcgggtggg cggagatggt gcggatgccg
     3241 ttttgtcgtc agccgatgtt gaggcgttcc atacggtagc gcatggaacg gaagctgatg
     3301 cccaagcgtt tggcggcctg cgtgcggttg ccttcggttt gtttgagggt ttgttcgatg
     3361 atgtcgcgtt cgatttggtc gagatagtcc tgtatctgca tggtatcggg gtcgaacgcg
     3421 ccgcaacgca acgcttcctg tgcctgatcg gcgttgccga aggcggtgat gacggcaacc
     3481 ggcgtatcga gcaggcggct gttgatgtgt tggacgactt ccagccccga gccgtccggc
     3541 atacgcatat cggtcaggac gagcgaatag cgttggctgt cgagcttgtt tttggcttcg
     3601 gcaacgcctg acgcggtatg gacgcgcaag cccattttca tcagggtcat ttccatcagg
     3661 tcgagaatgt cggtttcgtc atcgacgacg aggacggggt gttgcagttc agtcattggt
     3721 tttttccggt aatgtgagtt cgaaacattt ggcttccggt aggtaggtca aatcgccgaa
     3781 attggcgtgc gccagttcgc gggcgacata cagcccgagg ccggtgccgt tttccgccgt
     3841 ggtgtaaaag ggttcgaaca agtgttcctg cacttcgggc ggaacgccgc cgccgtcgtc
     3901 ggcaaacagg atgctgatgg tgtttttttg tgcaggacgg gcgacgacgg caatcgagcc
     3961 gttacgtttg ctgctgtgcc gccacgcgtt gttggcgagg ttccacataa tttgccgcag
     4021 gtgggcagga tcgaaatagg cagtcaggtg attgccctgc atatctaggc ggatgcagcc
     4081 ggcggcatcg ggatggccga gcaggaactc ttgtttgaat tcttcccaaa acggtatcag
     4141 gccgatggtt tcgcgttcgg ttttgttgcg cttgttggcg aggaaatatc ttcgagcatt
     4201 ttgtcgatac ggcagacgtt gccgtcgatg attttgcaca atttggcgtt gaacggatct
     4261 gccgcccccg cagcctttga tgcgccggta ggcgaggtgg ttgtcgcgtg ccagccggcc
     4321 ggcgctgtcg atgtatctga ccgacagtga ggtgaagatg gcggaaagat aggaggcggc
     4381 gacaaggatg aaggttaccg aggcggttcg ggcatccgat atgtgcggat atttgccgat
     4441 atcgccgtcg gcaatggcgt tgaatatcag caggatggcg gcgtagctgg catagagcag
     4501 ggggtaacgc ccgtagctga gcaggcagga gcagacgacg aagggcagaa tcaggatgcc
     4561 gaagccggag tcgatgccgc cgaacaggta ggtcagcacg ccgatcatcg tgatgtccgc
     4621 taccgcgctg aaactcggta ttctcaaagc ctgccattgc caatgcggat ggatgatgga
     4681 aaagaaaatc agccagcagg caatggcgag ccaaacataa aacgcccacg ccgtccacga
     4741 agcgtgcagg ttcgcacggc tgccggtttc cagcccgagg atgtgcataa tcatcagcgg
     4801 aaaaacgatg gcgacgcgga tgatgttgat cagattggga atccggtctt tgagtttttc
     4861 aagttcgcgg gggttggaaa tcaccataat atgtgcggaa ccgggtttat ttaggggaag
     4921 aaagctgttt caggctgccc gatatgggca atagtctgcc ttttttgccg cgttttgcct
     4981 caagcaggga gatggggatg cggtctttgt gcgccgcgcc gcgcctgcct tcgctttcaa
     5041 tcaggatttc cagctcggaa gaaacggcgg tatgcgtcat cgattcgcca gcgtttaatc
     5101 cgatgatttg cagccctttg cctttcgcca taattttcaa ttcgccgatg gggaaggcga
     5161 gggcgcggtt ttgactggtg gcggcaatga ttttgcagtc ggggttgatg aacgaggagg
     5221 catagacggc aaccggcggc aaaacggttt cgccgctgtc tgcggtcatc accactttgc
     5281 ccgctttcac gcgcccgacc atatcgccca gcttggtgat gaagccatag ccgctgctgc
     5341 ttgataataa ataatgttgt tccggcaatc ctgtcaacat cgcaacgggt ttcgcgccgt
     5401 tttgcagctc gattaaggag gaaaccggta cgccgtcgcc gcgccctccg gggatttcgg
     5461 cggcatcgag cgtgtaggtt ctgcccgatg aatcgaggat gacgacgggt aaaaccgttc
     5521 tgccttcgag ggtttgtttg aggcagtcgc cttctttgaa cgcggtttgg ctcaaatcga
     5581 gattgtgtcc ggcgcggctg cgtatccagc ctttttccga caggatcagc gtgatgggtt
     5641 cgtcggcggt ggtctgcgtc agcacggcgc gtccggcctc ttccaccagc gtgcgtcgcg
     5701 cgtcgccgta ttgtttcata tccgcctgca tctctttgac aatcagcttg cgtttttcgt
     5761 tttcgtcgct caaaaggatg ttcagacggc cttgttcctc gcgcaactcg ttcaattctt
     5821 tttcgagttt gaaaccctcc aaacgcgcca actggcgcag gcggatttcc aaaatgtctt
     5881 cggcttggat ttcggtcagc ccgaacgccg ccatcaaatc ggctttcggg tcatctgatt
     5941 cgcggatgac tttaatcact tcgtcgatgt gcagaaagac tttcagacgg ccttcgagga
     6001 tgtgcagccg tttttccact tggttcaaac ggaatttcag acggcgtgtt acggttacgg
     6061 tgcggaaatc cagccattcc tgcaaaatcg ttttcaggtt tttctgcgcg gggcggttgt
     6121 ccagccccat catcaccaag ttcatggaca cattgccttc cagcgaagtt tgcgccatca
     6181 gcgtgttgat gaaggtatcg gtatcgatgc ggctggattt tggttcaaat acaaggcgca
     6241 cgggatgttc gccgtcggac tcgtcgcgca cgcggtcgat taaatccagc atcagctttt
     6301 tggtattgag ctggtcttgg ttgagctgct ttttgcccgc tttcggtttc gggttggtct
     6361 gctcttcgat ttcggcaagg attttggcgg aattggcgtt cggcggtagc tcggttacga
     6421 tgacgcgcca ttgtccgcgc gccaattttt cgatttcata acgcgcacgc acgcgcacgc
     6481 tgcccttgcc ggtttcataa atccggcgca attcgtccgc cggcgtgatg atttgaccgc
     6541 cgccggcaaa atcaggtgcg ggaatgtatt gcatcaggtc ggcggtttcc agcgtcggct
     6601 ttttcaacaa cgcaatcgcc gcctgcgtaa cttcgttcaa attgtgcgac ggaatctcgg
     6661 tcgccatgcc caccgcaatg cctgacgcgc cgttgagcaa caccataggc aggcgggcgg
     6721 gcaggtgcag cggttcgtca aacgcgccgt cgtagttcgg cacgaaatcc accgtcccct
     6781 gattgatttc ggacaacagc aattccgcaa tcggcgtgag acgcgcctcg gtgtaacgca
     6841 tcgccgccgc cccgtcgccg tcgcgcgaac cgaagttgcc gatgccgtcg atcaaggggt
     6901 agcgcaaggt aaaatcctgc gccatccgca ccatcgcctc ataggcggaa ctgtcgccgt
     6961 gcgggtggta tttgcccaaa atctcgccga ccacgcgcgc cgatttcacc ggcttcgccc
     7021 ccgccgtcaa acccatatcg cgcatcgcaa acaaaatgcg ccgctgcacg ggtttttggc
     7081 cgtctgaaac ttcaggcagc gcgcgacctt taaccacgct catggcgtat tcgagatagg
     7141 cgcgttcggc gtaccggccg agcatcagcg tattggaatc ggtatgtggg gcgtgcgctt
     7201 gcgtattcat tgtgtatgcg gaatgtaaaa caaagggcat tattgtaaat caaaacggca
     7261 aaccatagtg ggcgcgtgtc tgaatctgaa tcaaatacgg gcaatgtgga aatatgttat
     7321 aaataaggct ttcccacttt tacacattgg agacgatatg gaactggtat ttatccgcca
     7381 cggacaaagc gaatggaacg cgaaaaacct gtttacaggc tggcgcgacg tgaagctgag
     7441 cgagcagggg cttgccgagg ctgccgccgc cggtaaaaaa ctgaaagaaa acggctatga
     7501 gttcgacatc gccttcacat ccgtcctgac ccgcgcgatt aagacctgca acatcgtttt
     7561 ggaagaatcc gaccaactgt tcgtaccgca aatcaaaacg tggcggctga acgaacgcca
     7621 ctacggccaa ctgcaaggcc tggacaaaaa acaaaccgcc gaaaaatacg gcgacgagca
     7681 agtccgcatc tggcggcgca gctacgacac cctgccgccg cttttggaca aagacgatga
     7741 gttttccgca cacaaagacc gccgctatgc ccatctgcct gccgatgtcg tacccgacgg
     7801 cgaaaacctg aaagtaacgc tggagcgcgt attgccgttt tgggaagacc aaatcgcccc
     7861 cgcgattttg agcggcaaac gcgtcttggt ggcggcgcac ggcaactccc tgcgcgcgct
     7921 ggcaaaacac atcgagggca tttccgacga agacattatg ggcttggaaa tcccgaccgg
     7981 tcagccgctg gtgtacaaat tggatgacaa tttgaaagtc atcgaaaaat tctacctgta
     8041 agggtgtgaa ataaaaaatg ccgtctgaag gcttgagcgt tcagacggca tttttgacgg
     8101 taaacgtagc aactgctttc gcgagaacga cgggatttta ggtttttgtt tttctgtttt
     8161 cgtgggaagg atgggtttta ggttgcggtc atttatcgga aaagcagaaa ccgctccgcc
     8221 gtcattcccg cgcaggcggg aatctagaac ctctgaattt tcagacaacc tttgaatatt
     8281 gccgccgtcc cgcgttctgg attcccgcct gcgcgggaat gacgatttat aagttcccga
     8341 aacgcccgaa aaaaccgaaa tccgacaggc aggattttcg ctttcgtggg gatgacggga
     8401 tttttgtaac cgtagcaact gcttttgcgg taatgacggg attttaagtt acggtaagtt
     8461 atcggaaaga cagaaacttc tccgccgtca ttcccgcgaa ggcgggaatc tgaacacgtc
     8521 cgtagggaaa cctatatccc gtcattccca tgaaagtggg aatccaggat gcaggggaaa
     8581 tcgttttatc cgataagttt ccgccccgaa aggtctggat tcccgcctgc gcgggaatga
     8641 cgggattttt gtaaccgcag ggtgggcttc agcctgccaa tcaacaaccg aaaatgaaga
     8701 aaaacggtgg gctgaagccc accctacgca attgcttttg cggagatgac ggggttttag
     8761 ggtgaagatg gtgggagttg gtgggagttg gtgggctgaa gcccacccta cgcaactatc
     8821 ctacgcaact actacgcaat tgccgccgcc ttgcggtaca gctcgaacag gaaggcggtg
     8881 cgttcggctt cgtcgtcggt gtttttgcct gttttgtagc cgtaggcttt atcgactgcc
     8941 ttgtcgaggg cggcgtgggc tttgtctagg gcggtatagc ctgcgtcggg cgcatagagt
     9001 tcggcgaggg tcggctcggg caaaccggct tcccgcgctt ctcggcggta ttgtccgcgc
     9061 gcgtcgagga cggcttgggc ggcggtttcg acggcggcgc ggagcgggtc ttggcggtcg
     9121 ttttcagacg gcatccggca gctttcgggg aaggggaagt tgttgtacac aacggtattg
     9181 gaatagcggt aacgactttc cagacggccg gcaacggtac gcataaaggc gttgtgcatg
     9241 gtggagctga ggatgccgaa gtggtagagg ctggcgtttg gaagggtaaa taccaaatcg
     9301 ctgacaactg tttcatatga tagataaccg atggggacga aacggcggct ttcggatgag
     9361 acgcgtggga taatcagaaa attgccgtct gaaggctggc ggattttttg aaaaagccac
     9421 ggggttgctg catctttttg agtttgtttg tcgctgctgg cttcgcgcat ggttttgact
     9481 gcctgaatac gggcttgaac ttggggcatc tgtttcaggt cgtggttgcg tttaacgtcg
     9541 gatacgccgt gaaaccacag gcaccaacgg gttttgccgt tgataaattc ctctgcgccg
     9601 acaaagggac ggatgtattg ttccgccaag ggttcggcgg caatcagggc ttctttttca
     9661 tcggttgaaa ggataaggtt gccgccttcg gtcggtttgc ttccgttgac catgtcaggt
     9721 tcgcaatgta cgggacggct gcgcttggca ataatcacat cgggcgcgtc aatcagatac
     9781 ggattgatgt tggccgcggc gtgttttacg ggttcgcctt tgatgtcggg atagtcgtag
     9841 agggtttttt cagacggcat ttgcggcttt tggcggaagc cgacgatgat gcagtggacg
     9901 gcggctttgc ctgcggcttg gctcgtccat tgaaaggtgc ggtgggcaaa gtggatttcg
     9961 atgtcttgct tcaaaaggct gccccaaagg atttcgacct gctggccttg gcagatggaa
    10021 ttggtggaaa caaatgccgt ctgaacttgg ggatgctgcg ccatgatacc ggcggctttg
    10081 acataccagt tgcagacgta atccaacagg ccgtagcctt tgatatggcc gcagatgctt
    10141 tcgaggtcgt ttttctgctc tttggtttgg taggtcgagc cgataaaggg cggatcgccg
    10201 aagatgtagt ccgccgcctg ccaaggtgtg cggaggctgt tggcgtggat gatttcggcg
    10261 gcgtcttcga gcggcagcgt atggcaggcg acttcgccgt cgaagcggat ttgcgtgcgg
    10321 atgttgcatt ggtggttttt gagccacatg gcggtgcggg cgatgaggac ggcaaattcg
    10381 tctatttcga tgccgtgaaa ctgtttcaga cggcattgga cggacgggct gtcgaataag
    10441 ccgcctgttt tgtctttgag cgcttcggcg atgatgtcgt cttcgagggc gcggatacgg
    10501 tcgtaggcga cgataaggaa gttgccgcag ccgcaggccg ggtcgaggaa ttgcaggttt
    10561 tggatttttt ggtagaaggc ggcgagtttt ttgtctttgt cgcgtttgag ggctttgacg
    10621 gcttcaaatt cggcgcgcag gttttctaaa aaaaggccgt tgatgacttt gtcgatgttg
    10681 gcggtttcgg tgtagtgtgc gcccgcttcg cgccgctctt gtgcgtccat gactgattgg
    10741 aagagcgtgc cgaagatttc gggactgat
//
LOCUS       DAXHSF010000054        10602 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.054, whole genome shotgun sequence.
ACCESSION   DAXHSF010000054 DAXHSF010000000
VERSION     DAXHSF010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10602
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.054"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            1..148
                     /locus_tag="ACFM8S_001029"
                     /pseudo
     CDS             1..148
                     /locus_tag="ACFM8S_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(158..1267)
                     /gene="mltB"
                     /locus_tag="ACFM8S_001030"
     CDS             complement(158..1267)
                     /gene="mltB"
                     /locus_tag="ACFM8S_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFC6314112.1"
                     /translation="MKKRKILPLAICLAALSACTAMEARPPRANEAQAPRADEMKKES
                     RPAFDAAAVFDAAAVPVSDSGFAANANVRRFVDDEVGKGDFSRAEWQDFFDKAAYKAD
                     IVKIMHRPSTSRPWYVFRTGNSGKAKFRGARRFYAENRALIDDVAQKYGVPAELIVAV
                     IGIETNYGKNTGSFRVADALATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGS
                     YAGAMGMPQFMPSSYRKWAVDYDGDGHRDIWGNIGDVAASVANYMKQHGWRTGGKILV
                     SATLAPGADVQAIIGEKTALTRTVADLKAYGIIPGEELADDEKAVLFKLETAPGVFEY
                     YLGLNNFYTVWQYNHSRMYVTAVRDIANSLGGPGL"
     gene            complement(1264..1446)
                     /locus_tag="ACFM8S_001031"
     CDS             complement(1264..1446)
                     /locus_tag="ACFM8S_001031"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314113.1"
                     /translation="MPSERGSAFRRHFRRRQADFGRRKRQRAAFCKAADCGGYRISRR
                     ALAPLSHLPYLFGTYL"
     gene            complement(1468..2895)
                     /locus_tag="ACFM8S_001032"
     CDS             complement(1468..2895)
                     /locus_tag="ACFM8S_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HFC6314114.1"
                     /translation="MIHTEPSAQPSTMDTAAFLKHIESAFRRIFSDGIDLMRYLPEDK
                     WLALKQAGLLLPFLDKKHGGRKGSQFEIQEVLRIAGHYGVPVTLRTGIEGALVLQPLQ
                     EFGDEAQIAQGLDMVFKGEGGGLGVTEPETSGAAIAREMQSYYEYTDGQTIYVNAAKY
                     WQGNSQSDFLLVAAKERKNGKLAKVIDLLLVPKTYIRCETLASEGLRAVRYAVNRIDA
                     EMPATAVMKLSQSDAAGLRAFQNIFIRSRLQLIGMTHGIMEYILENLERYVRNDIRFV
                     DYERREIRRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVKTLATEYTYAAAQMLQK
                     LLGAKGFERGHTAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRATAEEKEAGMKLDKN
                     QTLLDRLQTDARFAAVARDYTLPEDIRSFLQEHTLTDACVLQKVFIGKIIARLFVFVQ
                     AEHEDAAACLLNDIRKDILDCRYCG"
     gene            3080..6778
                     /gene="mfd"
                     /locus_tag="ACFM8S_001033"
     CDS             3080..6778
                     /gene="mfd"
                     /locus_tag="ACFM8S_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFC6314115.1"
                     /translation="MTYPIPKPREKSRWSNLSQGSLPLALARYLPHKRLKVVLTQDAE
                     QALRLQTAWRFFRPHDTAVFLPDWETLPYERFSPHQDLVSERLSALWQIKSGAADVLF
                     VPVATAMQKLPPVPFLAGRTFWLKTGQTLDIGRLKTDLVDAGYNHVSHVVAAGEFAVR
                     GGIVDLFPMGSEMPYRIDLFDDEIDSIKTFDTDTQRTISPVSEIRLLPAHEFPTDSEA
                     QKIFRSRFREEVDGNPNDAAVYKAVSNGHFGAGVEYYLPLFFENELETLFDYIGEDAL
                     FVSLGDVHAEANRFWSDVKSRYAMAQGDETYPPLLPQHLYLSADVFAGRLKNYGQVLP
                     DVSGKEHTLPDLAVNRQSDEPLQALKDFQTTFDGRILLCAESLGRRETMLGFLQQNGL
                     KAKPVSDWQGFLSAHEPLMITVAPLAYGFKLGGLQSPNQQQTTSASEGEGDAVTDQTE
                     FSASATNPLPSPLPQEREQSAAVVSDSLKAAAVSTESSLPPGKSNLHGQIQQQPAPSP
                     VGEGWGEGKAVAAQSAIAVITESDLYQYVARSRVHNRRKKHAAVSDGLLRDLAEINIG
                     DPVVHEEHGIGRYMGLVTIDLGGETNEMMLLEYAGEAQLYVPVSQLHLISRYSGQAHE
                     NIALHKLGSGAWNKAKRKAAEKARDTAAELLNLYAQRAAQSGHKFEINELDYQAFADG
                     FGYEETEDQAAAIAAVIKDLTQAKPMDRLVCGDVGFGKTEVALRAAFVAVMGGKQVAV
                     LAPTTLLVEQHAQNFADRFADFPVKVASLSRFNNSKATKAVLEGMADGTVDIVIGTHK
                     LVQDDIKFKNLGLVIIDEEHRFGVRQKEQLKRLRANVDILTMTATPIPRTLSMALEGL
                     RDFSLITTAPSRRLAVKTFVKPFSEGSVREAVLRELKRGGQVFFLHNEVDTIENMRER
                     LETLLPEARIGVAHGQLRERELEQVMRDFLQQRFNVLLCSTIIETGIDIPNANTIIIN
                     RADKFGLAQLHQLRGRVGRSHHQAYAYLLTPEYITKDAEKRLDAIAAADELGAGFTLA
                     MQDLEIRGAGEILGEGQSGEMIQVGFTLYTEMLKQAVRDLKKGRQPDLDAPLGITTEI
                     KLHSPALLPESYCPDIHERLVLYKRLAVCETVQQINAIHEELVDRFGLPEQPVKTLIE
                     SHHLRLMAKELGIDAIDAAGEAVTVTFGKNNNVDPTEIILLIQNDKKYRLAGADKLRF
                     TAEMENIEVRINTVKNVLKTLQNRCLPK"
     gene            6927..7310
                     /gene="panD"
                     /locus_tag="ACFM8S_001034"
     CDS             6927..7310
                     /gene="panD"
                     /locus_tag="ACFM8S_001034"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFC6314116.1"
                     /translation="MFRTMLGGKIHRATVTEADLNYVGSITVDQDLLDAAGIYPNEKV
                     AIVNNNNGERFETYTISGKRGSGVVCLNGAAARLVQKGDIVIIMSYVQLSEPEIAAHE
                     PKVVLVDGNNKIRDIISYEPPHTVL"
     gene            7332..8174
                     /gene="kdsA"
                     /locus_tag="ACFM8S_001035"
     CDS             7332..8174
                     /gene="kdsA"
                     /locus_tag="ACFM8S_001035"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFC6314117.1"
                     /translation="MDIKINGITVGNDAPFVLFGGINVLEDLDSTLQTCAHYVEVTRK
                     LGIPYIFKASFDKANRSSIHSYRGVGLEEGLKIFEKVKAEFGIPVITDVHEPHQCQPV
                     AEVCDVIQLPAFLARQTDLVVAMAKTGNVVNIKKPQFLSPSQMKNIVEKFHEAGNGKL
                     ILCERGSSFGYDNLVVDMLGFGVMKQTCGNLPVIFDVTHSLQTRDAGSAASGGRRAQA
                     LDLALAGMATRLAGLFLESHPDPKLAKCDGPSALPLHLLEDFLIRIKALDDLIKSQPI
                     LTIE"
     gene            8189..8629
                     /locus_tag="ACFM8S_001036"
     CDS             8189..8629
                     /locus_tag="ACFM8S_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HFC6314118.1"
                     /translation="MMQTFRKISLYAATLWLGMQIMAGYIAAPVLFKMLPKMQAGEIA
                     GVLFDILSWSGLAVWGAVLAAAFAALTRRQTALLLFLLSALAANQFLVTPVIEALKYG
                     HENWLLSFVGGSFGMWHGISSMIFMATALLSAALSWRLSGKDAV"
     gene            8677..9963
                     /gene="eno"
                     /locus_tag="ACFM8S_001037"
     CDS             8677..9963
                     /gene="eno"
                     /locus_tag="ACFM8S_001037"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFC6314119.1"
                     /translation="MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     QKEALELRDGDKSRYSGKGVLKAVEHVNNQIAQALIGIDANEQSYIDQIMIELDGTEN
                     KGNLGANATLAVSMAVARAAAEDSGLPLYRYLGGAGPMSLPVPMMNVINGGEHANNSL
                     NIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEA
                     LQLMVEATEAAGYKAGEDVLFALDCASSEFYKDGKYHLEAEGRSYTNAEFAEYLESLV
                     NEFPIISIEDGMDENDWEGWKLLTEKLGGKVQLVGDDLFVTNPKILAEGIEKGVANAL
                     LVKVNQIGTLSETLKAVDLAKRNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSL
                     SRSDRMAKYNQLLRIEEELAEAAYYPGKAAFYQLGK"
     gene            9978..10256
                     /gene="ftsB"
                     /locus_tag="ACFM8S_001038"
     CDS             9978..10256
                     /gene="ftsB"
                     /locus_tag="ACFM8S_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFC6314120.1"
                     /translation="MKWVTVVLSFALVCCQYSLWFGKGSIGRNNNLKNQIAVQEEKNQ
                     TLALRNHSLAAEVYDLENGQEAISEIARVELGYIQDGETFYRLIRHNR"
     gene            complement(10297..10587)
                     /gene="yfaE"
                     /locus_tag="ACFM8S_001039"
     CDS             complement(10297..10587)
                     /gene="yfaE"
                     /locus_tag="ACFM8S_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I ribonucleotide reductase maintenance
                     protein YfaE"
                     /protein_id="HFC6314121.1"
                     /translation="MARIGTNKGFFELLEGETLLEGLERTGHMVEYQCRSGYCGSCRV
                     KILEGSVTYREPPLAFLGRDEILPCCCCVEGDVRLDCGLSGEDEGLSDSLLK"
BASE COUNT         2535 a         3093 c         2710 g         2264 t
ORIGIN      
        1 tgcaaaaatg ccgtccgaac cgaaacgtgg ttcagacggc attttatagt ggattaacaa
       61 aaatcaggac aaggcgacga agccgcagac agtacaaata gtacggcaag gcaaggcaac
      121 gccgtactgg tttaaattta atccactata aaaaacatta caatcccggg ccgccaagcg
      181 aattggcaat gtccctgacc gccgttacat acatccgact gtgattgtac tgccataccg
      241 tataaaaatt gttcaagccc aaataatatt caaacacgcc gggtgcggtt tccagtttga
      301 acaaaaccgc cttttcatca tctgcaagct cttcgccggg gatgatgccg tacgccttta
      361 aatccgccac cgtccgcgtc agggcggttt tttcgccaat gattgcctga acatccgcac
      421 ccggcgccaa tgttgcagac accagtattt tcccgcccgt gcgccaaccg tgctgcttca
      481 tataattggc aaccgatgcc gcgacatcgc cgatgttgcc ccatatgtcc cgatgtccgt
      541 ccccgtcata atccaccgcc catttccggt agctcgaagg cataaattgc ggcatcccca
      601 ttgcgcccgc atagctgcct ttaaaggcga aaacatcgcc gccttcttct tttgccagct
      661 ttaaaagctc gaccaattct ttttggaaaa acccggcgcg gcgggggtaa tcaaagccta
      721 aggtcgccaa tgcgtccgcc acacggaaac tgcccgtatt tttgccgtaa ttcgtttcaa
      781 tcccgataac cgccacgata agttcggcag gcacgccgta tttttgcgcc acatcatcga
      841 taagcgcgcg gttttccgca taaaaccggc gcgcgccgcg aaatttcgcc ttgcccgaat
      901 ttcccgtgcg gaacacatac cacggacgcg atgtggaggg gcggtgcata atcttgacga
      961 tgtccgcctt gtaagccgct ttgtcaaaaa aatcctgcca ttccgcccgg gaaaaatccc
     1021 ctttcccgac ttcatcgtcc acaaaacggc ggacatttgc attggcggca aacccgctgt
     1081 cggataccgg tacggctgcc gcgtcaaata cggctgccgc gtcaaacgcg gggcggcttt
     1141 cttttttcat ttcatccgcg cggggggctt gggcttcatt tgcccggggc gggcgtgcct
     1201 ccatcgccgt acaggcagac aaagccgcca aacaaattgc cagcggcagt atttttctct
     1261 ttttcataaa tatgttccga acaaataggg taagtgggaa agcggcgcaa gggcgcggcg
     1321 cgaaatgcgg tatccgccgc aatcggcggc tttgcagaat gccgcgcgtt gccttttgcg
     1381 ccgcccgaaa tctgcctgcc gccgccgaaa atgccgtcta aaggcacttc ccctttcaga
     1441 cggcattgcc tgcgccgtat ttgcccgcta tccgcaatat cggcagtcca atatgtcttt
     1501 gcggatatcg ttcagcaggc aggctgccgc gtcttcgtgt tccgcctgta cgaagacaaa
     1561 gagtcgggcg atgattttgc cgataaagac tttttgcagg acgcaggcgt cggtcagcgt
     1621 gtgttcctgc aggaagctgc ggatgtcttc gggtaaggtg taatcacggg caacggcggc
     1681 aaagcgggca tcggtttgca ggcggtcgag cagggtttgg tttttgtcca acttcatgcc
     1741 tgcttctttt tcttcggcgg tggcgcggac aaactggtcg taaatttcgg cataaagcat
     1801 atcgttcggg ccttcaaaaa tcgtgaaggg gcggatgtcg atagcgatat tgccggcggt
     1861 gtgtccgcgt tcaaaaccct tcgcgcccaa gagtttttgc aacatttgcg cggcggcgta
     1921 agtgtattcc gtggcgaggg ttttgacgat gttcgcctcc atcagctgat gggcgacggg
     1981 ggcaacaggc gaaacggaat ggcagacgta gcggtaaaga atctcggaaa cctgatggcg
     2041 gcgccggatt tcgcggcgtt cgtaatcgac gaatctgatg tcgttgcgga cgtatcgttc
     2101 caggttttca aggatgtatt ccataatgcc gtgcgtcatg ccgatcagtt gcaggcggct
     2161 gcggataaag atgttttgga acgcgcgcaa accggcagcg tcgctctggg agagtttcat
     2221 cacggcggtt gcaggcattt cggcatcgat gcggttgacg gcgtaacgga cggcgcgcaa
     2281 gccttcggat gcgagggttt cgcagcggat gtatgttttg gggacgagca gcaggtcgat
     2341 gactttggcg agtttgccgt ttttgcgttc tttggcggca acgaggagga agtcgctttg
     2401 cgagttgccc tgccagtatt ttgcggcgtt gacgtaaatg gtttgtccgt cggtatattc
     2461 gtagtaagac tgcatttctc gggcaatcgc cgcgccggag gtttcgggtt cggtaacgcc
     2521 caaaccaccg ccctcgcctt tgaaaaccat gtccaaaccc tgtgcgattt gcgcttcatc
     2581 gccgaactct tgcagtggct gcaacaccag cgcgccttcg atgccggtac gcagcgtaac
     2641 gggcacgccg taatgccccg caatccgcaa gacttcctga atttcaaact ggctgccctt
     2701 gcgcccgccg tgttttttgt cgaggaaggg caacagcaaa cccgcctgct tcaaggcaag
     2761 ccatttgtct tcgggcaggt atcgcatcag gtcgataccg tctgaaaaaa tgcggcggaa
     2821 tgcggattcg atgtgcttta aaaaagcagc cgtgtccata gttgacggct gcgcgctcgg
     2881 ttcggtgtgt atcatcggct tcctctgtcg gttcccatta atcggcggcc ggtcaaaccg
     2941 cctgccacag tttagagttg attttctaaa ctttaccaca aagtgcgccg ggcaacaatc
     3001 cgccgacctt tcagacggca tcgcgcgccc tcccgtgcta aaatgaccgt ttgcatcact
     3061 gtccgccgat tgccgcacta tgacctaccc catccccaaa ccccgtgaaa aatcccgttg
     3121 gtccaatctt tcgcaaggct cgctgccctt ggctttggcg cgttatctgc cgcataagcg
     3181 gctcaaggtc gtgctgaccc aagatgcgga acaggcgcta cgccttcaga cggcatggcg
     3241 gtttttccgt ccgcacgata cggcggtgtt cctgccggac tgggaaacgc tgccttacga
     3301 gcgtttttcg ccgcatcagg atttggtgtc ggagcggctg tcggcgttgt ggcagattaa
     3361 gagcggcgcg gcggatgtgt tgtttgtacc ggttgccacg gcgatgcaga agctgccgcc
     3421 cgtgccgttt ctggcagggc gcacgttttg gctgaaaaca ggacagactt tggatatagg
     3481 ccgtctgaaa accgatttgg tagatgcggg ctacaaccat gtttcccatg ttgttgcggc
     3541 gggcgagttt gccgtgcgcg gcggcattgt cgatttgttc cccatgggca gcgaaatgcc
     3601 ctatcgcatc gatttgtttg acgatgaaat cgacagcatc aaaacctttg atacggacac
     3661 gcagcgcacc atctcccccg tttccgaaat ccgcctgctg ccggcgcacg agttccccac
     3721 cgacagcgaa gcgcaaaaaa tcttccgcag ccgcttccga gaagaagtcg acggtaatcc
     3781 gaacgatgcg gctgtgtaca aagccgtcag caacggccac ttcggcgcgg gcgtggaata
     3841 ttatctgccg ctgttttttg aaaatgagct ggaaacgctg tttgactata tcggcgaaga
     3901 cgcgctgttt gtctctttgg gcgatgttca cgccgaggca aaccgctttt ggagcgatgt
     3961 caaatcccgt tacgcgatgg cgcagggcga cgaaacctat ccgcctttgc ttccacagca
     4021 tttgtatctc tctgctgatg tttttgcagg ccgtctgaaa aactacggac aagtgctgcc
     4081 cgatgtttcc ggcaaggaac acaccctgcc tgaccttgcc gtcaaccgcc aatccgacga
     4141 gccgttgcag gcattgaagg attttcagac gacctttgac gggcggattc tgctgtgcgc
     4201 cgaaagcttg ggacggcgcg aaaccatgct cggtttcttg cagcaaaacg gtttgaaagc
     4261 caaacctgtg tccgactggc agggcttctt gtcggcgcat gagccgctga tgattacagt
     4321 ggcgccgttg gcttacgggt ttaaactggg cggactgcaa tcgccgaacc aacagcaaac
     4381 tacttccgcc tccgaaggag aaggcgatgc ggttaccgac caaaccgaat tttccgcatc
     4441 cgcaacaaac cctctcccta gccctctccc acaggagagg gaacaaagtg cagccgtcgt
     4501 ttcagacagt ctgaaagcag ccgccgtttc aaccgaaagc agcctgcccc ccggtaaaag
     4561 taatctgcac gggcaaatcc aacagcaacc tgccccctcc cccgtggggg agggttgggg
     4621 agagggcaaa gcagttgccg ctcaaagtgc catcgccgtc atcaccgaat ccgatctcta
     4681 ccaatacgtc gcccgttcgc gcgtccacaa ccgccgcaag aaacacgccg ccgtttcaga
     4741 cggtctgttg cgcgaccttg ccgaaatcaa catcggcgac cccgtcgttc acgaagaaca
     4801 cggcatcggg cggtatatgg gcttggtaac gatagatttg ggcggcgaaa ccaacgaaat
     4861 gatgttgctc gaatatgcag gcgaagcgca gctttatgtg cctgtttcgc aactgcattt
     4921 aatcagccgc tactccggtc aggcgcatga aaacattgcc ctgcacaagc tcggcagcgg
     4981 cgcgtggaac aaggcaaaac gcaaggctgc cgaaaaagcg cgcgataccg ccgccgagtt
     5041 gctcaatctc tatgcccaac gcgccgccca atcgggacac aagtttgaaa tcaacgagtt
     5101 ggattatcag gcgtttgccg acggcttcgg ctacgaagaa accgaagacc aggcagccgc
     5161 catcgccgcc gtgattaaag atttgacgca agcgaagccg atggatcgcc ttgtgtgcgg
     5221 cgatgtcggc ttcggcaaaa ccgaagtcgc cctgcgcgcc gcgtttgtgg cggtgatggg
     5281 cggcaaacag gtcgccgtac ttgctccgac cacgcttttg gtcgagcagc acgcgcaaaa
     5341 cttcgccgac cgtttcgccg atttccccgt gaaagtcgcc agcctttcgc gtttcaacaa
     5401 cagcaaagcc accaaagccg tgctggaagg catggcagac ggcacggtcg atattgttat
     5461 cggtacgcac aaactggtgc aggacgacat caaattcaaa aacttaggtt tagtgattat
     5521 cgacgaagaa caccgcttcg gcgtgcgtca gaaagagcag ctcaaacgcc tgcgcgccaa
     5581 tgttgatatc cttaccatga ccgccacgcc gattccgcgt actttaagta tggcgttgga
     5641 aggactgcgc gacttctcgc tgattaccac tgcgcccagc cgccgcctcg ccgtcaaaac
     5701 ctttgtcaaa ccgttcagcg aaggcagcgt gcgcgaagcc gtattgcgcg aactcaaacg
     5761 cggcgggcaa gtatttttcc tgcacaatga agtggatacc atcgaaaaca tgcgtgagcg
     5821 gctggaaacc ctgctgcccg aagcccgcat cggcgtggcg cacggacaac tgcgcgagcg
     5881 cgagctggag caagtgatgc gcgacttttt gcagcaacga tttaacgtgt tgctctgttc
     5941 caccatcatc gaaaccggta tcgacattcc caacgccaac accatcatca tcaaccgtgc
     6001 cgacaaattc ggattggcgc agctgcacca gcttcgcggg cgcgtcggac gcagccacca
     6061 ccaagcctac gcctacctgc tcacgcccga atacatcacc aaagacgcag aaaaacgcct
     6121 cgacgccatc gcggcagcag acgaactcgg cgcaggtttc accctcgcca tgcaggattt
     6181 ggaaatccgc ggcgcaggcg aaatcctcgg cgaagggcag tccggcgaaa tgatacaggt
     6241 cggcttcacg ctctacaccg aaatgctcaa acaagccgtg cgcgacctca aaaaaggccg
     6301 ccagcccgac ctcgacgcac cgctgggcat caccaccgaa atcaaactgc acagccccgc
     6361 cctgctgccc gaaagctact gccccgacat ccacgagcgt ctcgtcctct acaaacgcct
     6421 cgccgtctgc gaaaccgtgc agcaaatcaa cgccatacac gaagaactcg tcgaccgctt
     6481 cggcctgccc gaacaacccg tcaaaaccct tatcgaaagc caccacttac ggcttatggc
     6541 aaaagaattg ggtatcgatg ccattgatgc ggccggcgaa gcggtaacgg taaccttcgg
     6601 taaaaacaat aatgtcgatc caaccgaaat catcctgctg attcagaacg acaaaaaata
     6661 ccgccttgcc ggcgccgata agctgcggtt taccgcagag atggaaaata tcgaggtcag
     6721 aatcaacacc gtaaaaaacg ttttaaaaac cttgcaaaac agatgcctgc ccaaataaag
     6781 ccgacaccgc aatgccgtct gaaacaccgt tttccttgtc cgaaagccgc cattatgaat
     6841 ttgaaggaaa ctccactata atacggcatt cagatttcca gacggcatcg cgcccgtcaa
     6901 accgcacaca aaccaaaagg aaatacatgt tccgtactat gcttggcgga aaaatccacc
     6961 gcgccaccgt taccgaagcc gatttgaact atgtcggcag tattaccgtc gatcaagacc
     7021 tgttagacgc agcaggcatc taccccaacg aaaaagtcgc catcgtcaac aacaacaacg
     7081 gcgaacgttt tgaaacctat accatttcag gcaaacgcgg cagcggcgtg gtctgcctga
     7141 acggggcggc ggccaggctg gtacagaaag gcgatatcgt catcatcatg tcttacgtcc
     7201 aactctccga acccgaaatc gccgcacacg aacccaaagt cgtcttggta gacggaaaca
     7261 acaaaatccg cgacatcatc tcctatgagc cgccgcacac cgtgctgtaa ccctgaaaac
     7321 ggacattgac catggatatt aaaatcaacg gcattaccgt cggtaacgat gctcctttcg
     7381 tactgttcgg cggcatcaac gttttagaag atttggattc caccctccaa acctgtgcgc
     7441 attacgtcga agttacccga aaactcggta ttccctatat ctttaaagcc tctttcgaca
     7501 aggcaaaccg ttcctccatc cattcttatc gcggcgtagg cttggaagaa ggcttaaaga
     7561 tttttgaaaa agtcaaagca gagttcggca tccccgtcat taccgacgta cacgaacccc
     7621 atcagtgcca acccgtcgcc gaagtgtgcg atgtcatcca gcttcccgcc tttcttgcgc
     7681 ggcagaccga tttagtggtt gccatggcaa aaactggcaa cgtcgtcaac atcaaaaaac
     7741 ctcagttcct cagcccctct caaatgaaaa acattgtgga aaaattccac gaagccggca
     7801 acgggaaact gattttatgc gaacgcggca gcagcttcgg ctacgacaac ctcgttgtcg
     7861 atatgctcgg tttcggcgtg atgaaacaga cttgcggcaa cctgccggtt attttcgacg
     7921 ttacccattc cctgcaaacc cgcgatgccg gttctgccgc atccggcggt cgtcgcgcac
     7981 aggctttgga tttggcactt gcaggcatgg caacccgcct tgccggcctg ttcctcgaat
     8041 cgcaccccga tccgaaactg gcaaaatgcg acggccccag cgcgctgccg ctgcaccttt
     8101 tagaagattt tttaatccgc atcaaagcat tggacgattt aatcaaatca caaccgattt
     8161 taacaatcga gtaacacggt ttcgccttat gatgcagact ttccgaaaaa tcagcctgta
     8221 tgccgcaacc ttgtggctcg gtatgcagat tatggcgggt tatatcgccg cgccggtgct
     8281 gttcaaaatg ctgcccaaaa tgcaggcggg cgaaattgcc ggcgtattgt tcgacatcct
     8341 ctcttggagc gggcttgccg tttggggcgc ggtactggct gccgcctttg ccgccctaac
     8401 ccggcggcaa accgccctgc tgcttttttt attgtccgcc cttgccgcca accaattttt
     8461 ggttaccccc gttatcgagg cactgaaata cgggcatgaa aattggctgt tgtcgtttgt
     8521 aggcggatcc ttcggaatgt ggcacggtat ttccagcatg attttcatgg caaccgccct
     8581 actttcagca gctttaagtt ggcggctttc cggcaaagat gccgtctgaa gccctcccat
     8641 ttttttacct cccttcactt cacttggaga acattcatga gcgcaatcgt tgatattttc
     8701 gcccgcgaaa ttttggactc acgcggcaac cccacagtcg agtgtgatgt attgctcgaa
     8761 tccggcgtaa tgggacgcgc agccgtaccg agcggcgcgt ccaccggtca aaaagaggct
     8821 ttggaacttc gcgacggcga caaatcccgt tattcgggca agggcgtatt gaaggcggtc
     8881 gaacacgtca acaaccaaat cgcccaagcc ctcattggta tcgatgccaa cgagcaatct
     8941 tatatcgacc aaatcatgat cgaattggac ggtactgaaa acaaaggcaa tttgggtgcg
     9001 aatgcgactt tggcggtttc tatggcggtt gcacgcgccg ctgccgaaga ctcaggcctg
     9061 ccgctttacc gctacttggg cggcgcaggc ccgatgtccc tgcccgtacc gatgatgaac
     9121 gtcatcaacg gcggcgaaca cgccaacaac agcctgaaca tccaagagtt tatgattatg
     9181 cccgtcggcg caaaatcttt ccgcgaagcg ttgcgctgcg gtgcggaaat tttccacgcc
     9241 ttgaaaaaac tgtgcgacag caaaggcttc ccgaccacag tcggcgacga aggcggtttc
     9301 gcccccaacc tgaacagcca caaagaagcc ctgcaactga tggtcgaggc gaccgaagcc
     9361 gccggctaca aagcgggcga agacgtatta ttcgcattgg actgcgcctc cagcgagttc
     9421 tacaaagacg gcaaatacca cttggaagcc gaaggccgct cctacaccaa cgcggaattt
     9481 gccgaatatc tggaaagtct ggtcaacgag ttccccatca tctccatcga agacggcatg
     9541 gatgaaaacg actgggaagg ctggaaactg ctgaccgaaa aactgggcgg caaagtccag
     9601 ctcgttggcg acgacctctt cgttaccaat ccgaaaatcc ttgccgaagg cattgaaaaa
     9661 ggcgtggcaa acgcactatt ggtcaaagtc aaccaaatcg gtactttgag cgaaaccctg
     9721 aaagccgtcg atctggcaaa acgcaaccgc tacgccagcg tgatgagcca ccgctccggc
     9781 gaaaccgaag acagcaccat tgccgacttg gcagtcgcca ccaactgtat gcagattaaa
     9841 accggttccc tgtcccgttc cgaccgtatg gcgaaataca accaactgct gcgtatcgag
     9901 gaagaattgg cggaagccgc ctactacccc ggcaaagccg cattctacca actgggcaaa
     9961 taagaaaagg ttaaggtatg aagtgggtaa ctgtcgtttt atccttcgca cttgtctgtt
    10021 gccaatacag cctctggttc ggcaaaggca gtatcgggcg caacaacaac ctgaaaaatc
    10081 agattgccgt tcaggaagaa aaaaaccaga cactcgccct acgcaaccat tcccttgcag
    10141 ccgaagtcta tgatttggaa aacggtcaag aagccatttc ggaaatcgcc cgggtagaac
    10201 tgggttatat ccaagacggt gaaacctttt accgactcat caggcataac cggtaatacc
    10261 gtcaaaaagc cgtccgaacc aatgttcgga cggcttttat ttcaacaaac tgtcagacag
    10321 cccctcatcc tcccccgaca aaccgcaatc cagcctgaca tccccctcga cgcaacagca
    10381 gcacggcagt atctcgtccc gccccaaaaa agccaaaggc ggctcccgat aagtaacgct
    10441 tccctccaaa atcttcactc ggcacgatcc gcaatatccg cttcggcact gatattccac
    10501 catatgcccc gtccgttcca agccttccaa cagagtctcg ccctccaaga gctcaaaaaa
    10561 acccttattc gtaccaatgc gcgccatttc cgaccaatca aa
//
LOCUS       DAXHSF010000055        10511 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.055, whole genome shotgun sequence.
ACCESSION   DAXHSF010000055 DAXHSF010000000
VERSION     DAXHSF010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10511
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.055"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            205..1638
                     /gene="lpdA"
                     /locus_tag="ACFM8S_001040"
     CDS             205..1638
                     /gene="lpdA"
                     /locus_tag="ACFM8S_001040"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC6314122.1"
                     /translation="MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPA
                     LGGTCLNVGCIPSKALLQSSEHFHAAQHEFAEHGITVGDVKFDAAKMIERKDAIVTKL
                     TGGVKFLFQKNKVTSLFGTASFAGKNGDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
                     LPQVAIDNVNVLDNEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGAEVTILEAAPT
                     FLAAADQQIAKEAFKYFTKEQGLSIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFD
                     KLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGECRTNLPNVWAIGDVVRGPMLAHK
                     ASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGF
                     GANGRALAMGKAKGTVKVLADTKTDRILGVHMIGPVVSELVTEGVTALEFFASSEDIA
                     RIIHAHPTLSEVVHEAALAADKRALHG"
     gene            1763..2050
                     /locus_tag="ACFM8S_001041"
     CDS             1763..2050
                     /locus_tag="ACFM8S_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314123.1"
                     /translation="MNKEIVGIFFIPMGIISMCMAALWQMYVMMTETYTLNRFKDKEL
                     VWRVALLFISFSLAVYLLCPNSRKKGIVFFILGGGGAAMYLLARMWLPFSK"
     gene            2104..3315
                     /gene="sucC"
                     /locus_tag="ACFM8S_001042"
     CDS             2104..3315
                     /gene="sucC"
                     /locus_tag="ACFM8S_001042"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFC6314124.1"
                     /translation="MEANSGHSTNLKEKSMNLHEYQAKELLASYGLPVQGGILAHNGE
                     EAAAAYDKLGGKFAVVKAQVHAGGRGKAGGVKVVKSREEAKEVAESLIGTNLVTYQTD
                     ANGQPVNSVLVCEDMYPVQTELYLGAVVDRSTRRVTFMASTEGGVEIEKVAAETPEKI
                     FKVTVDPLVGLQPCQAREVAFQLGLKDKQINEFVKLMTGAYKAFVENDFALFEVNPLA
                     VRENGALACVDGKIGIDSNALYRLPKIAELRDKSQENERELKASEFDLNYVALEGNIG
                     CMVNGAGLAMATMDIIKLKGGQPANFLDVGGGATKDRVVEAFKLILEDKSVKGVLINI
                     FGGIVRCDMIAEAIVAAVKEINVDVPVVVRLEGNNAELGAKILNESGLKLTSADGLND
                     AAEKIVAAVNA"
     gene            3326..4216
                     /gene="sucD"
                     /locus_tag="ACFM8S_001043"
     CDS             3326..4216
                     /gene="sucD"
                     /locus_tag="ACFM8S_001043"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFC6314125.1"
                     /translation="MSVLINKDTKVLVQGFTGKNGTFHSEQALAYGTKVVGGVTPGKG
                     GQTHLNLPVFNTMKEAVKETGADASVIYVPAPFVLDSIVEAVDSGVGLVVVITEGVPT
                     LDMLKAKRYLETNGNGTRLVGPNCPGVITPGECKIGIMPGHIHTPGRIGIISRSGTLT
                     YEAVAQTTKLGLGQSTCIGIGGDPIPGMNQIDALKLFQEDPDTDAIIMIGEIGGTAEE
                     EAAEYIQSNVTKPVVGYIAGVTAPKGKRMGHAGAIISGGKGTAEEKFAAFEKAGIAYT
                     RSPAELGTTMLEVLKAKGLA"
     gene            4245..4460
                     /locus_tag="ACFM8S_001044"
     CDS             4245..4460
                     /locus_tag="ACFM8S_001044"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314126.1"
                     /translation="MPSETGNRVSDGILFVISKRGSLNIPTLFFPFWGSQRPLQNSPK
                     SPKFPPRHLGDFGEFCKGLGLSVPVCA"
     gene            complement(4767..6362)
                     /locus_tag="ACFM8S_001045"
     CDS             complement(4767..6362)
                     /locus_tag="ACFM8S_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="funZ protein"
                     /protein_id="HFC6314127.1"
                     /translation="MKPLKTLEFGFVDAANYRRRENKDLFNRIFVKGEYLDELCEPNI
                     SFLIGEKGTGKTAYAVYLTNNFYKNIHATTKFVRETDYSKFIQLKKARHLTVSDFTSI
                     WKVILYLLISNQIKCKENGILSSIFNKFKALDEAINEYYYGAFDPEIVQAITLIENSK
                     EAAEMIFGKFVKLGEEESQQITFTESKFQANLGFIERKFKDALSQLKLKDNHILFIDG
                     IDIRPSQIPFDEYHECVKGLANAIWMLNNDIFPSIKDSKGRMRVVLLIRPDIFDSLGL
                     QNQNTKLQDNSVFLDWRTDYKSYRSSKIFGVFDHLLRTQQEKQDSLEKGNSWDYYFPW
                     NAPNLHDEYKNLTSFISFLRKSYYRPRDILQMLTLLQKNKKSKEDYVVAEDFDNTSFQ
                     REYSIYLLGEIKDHLLFYYSQSDYQNFLKFFEFLNGKDRFKYSDFLKAFERLKKHLQT
                     TSVEIPKFMSTANEFLQFLFDLNVIAYLDNPEDETKPYIHWCFKDRNYANISPKIKTE
                     TEYLIFSGLSKALDVGTPFKNKQ"
     gene            complement(6396..9245)
                     /gene="uvrA"
                     /locus_tag="ACFM8S_001046"
     CDS             complement(6396..9245)
                     /gene="uvrA"
                     /locus_tag="ACFM8S_001046"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFC6314128.1"
                     /translation="MCKHHPQHSHDNDTIRIRGARTHNLKNIDLDIPRHKLVVVTGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQMMDKPDVDLIEGLSPAISIEQKSTS
                     HNPRSTVGTVTEIHDYLRLLYARVGTPYCPEHNLPLSSQTVSQMVDAVLKLPEDTRVM
                     ILAPAVRERKGEFVDFFADLQAQGFARVRVDGEVYQLDEVPKLEKNIKHNIDVVIDRV
                     KVKANIKQRLAESFETALRHGNERALAMEMDSGEEHWFSARFACPVCSYSLPELEPRL
                     FSFNNPMGSCPTCDGLGNTNFFDPEKVVAHPELSLATGAIDGWDKRNQFYFQMIQSLA
                     RHYGFDVQAAWETLPEKVKKVVLHGSGKEVIDFTYLSERGTTFNRSHAFEGIIPNLER
                     RYRETDSETVREKLREYQNHRACPSCGGARLRKEARYVYVSGEPLHEVSAWPLTKTHR
                     FFETLDLDGNKKQIAEKILKEITERLGFLINVGLDYLNLSRSAETLSGGEAQRIRLAS
                     QIGSGLTGVMYVLDEPSIGLHQRDNDRLLATLKRLRDLGNSVIVVEHDEDAIREADFV
                     VDMGSGAGEHGGNVLIADTPENVAQCENSVTGQYLSGKKSIAVPSERTPVNPGRMLVL
                     KGARGNNLKNVTLELPLGLITCITGVSGSGKSTLINDTLAKITARELNRAQEEPAPYD
                     DIRGLEHLDKVINVDQSPIGRTPRSNPATYTGLFTPIRELFAGVPLSRERGYNVGRFS
                     FNVKGGRCEACQGDGVIKVEMHFLPDVYVPCEVCHGKRYNRETLEIQYKGKNISQVLD
                     MTVEEAREFFDAVPTVSRKLQTLMDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRD
                     TGRTLYILDEPTTGLHFADIALLLEVIGRLKGKGNSIVIIEHNLDVIKTADWIVDLGP
                     EGGDGGGRIIACGSPEQVAKVKGSYTGKYLKVVLR"
     gene            9400..10197
                     /locus_tag="ACFM8S_001047"
     CDS             9400..10197
                     /locus_tag="ACFM8S_001047"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HFC6314129.1"
                     /translation="MNRLYPHPIIAREGWPIIGGGLALSLLVSMCCGWWSLPFWVFTV
                     FALQFFRDPAREIPQNPEAVLSPVDGRIVVVERARDPYRDVDALKISIFMNVFNVHSQ
                     KSPADCTVTKVVYNKGKFVNADLDKASTENERNAVLATTASGREITFVQVAGLVARRI
                     LCYTQAGAKLSRGERYGFIRFGSRVDMYLPVDAQAQVAIGDKVTGVKTVLARLPLTAP
                     QIESEPESEPASQAASVETAANPSAEQRQIEATAAKIQAAVQDVLKD"
BASE COUNT         2684 a         2441 c         2675 g         2711 t
ORIGIN      
        1 gatgtagcgt aatgtagtat cgtgctacaa taggctcaac gaacgattga ggccgtctga
       61 aacatttgat tcgaatgaat cggcagatat ggactttcag acggcttttt cttaaaacca
      121 tcaaaacgca gtcattcaaa ataaaaaaga aacgaaaagt atcgttttta ttttgagata
      181 ctgctaaaag caaaggatga cacgatgtct caatatgatg tagtagtgat tggtgcaggc
      241 ccgggcggat acgttgccgc catccgtgcc gcacaactgg gtttcaaaac tgcctgtgtc
      301 gatgcaggcg ttaacaaagc aggcaatgcc cctgcattgg gcggtacttg cttgaacgta
      361 ggctgtatcc cttctaaagc cctgttgcaa tccagcgaac atttccacgc agcgcaacac
      421 gagtttgccg aacacggtat cactgtcggc gacgtaaaat tcgacgcggc caaaatgatt
      481 gagcgcaaag atgccatcgt gaccaaactg accggcggcg tgaaattcct gttccaaaaa
      541 aacaaagtaa ccagtctgtt cggtacggct tcctttgctg gtaaaaatgg cgatgcttac
      601 caaatcgaag tcgataacaa aggcgagaaa accgttatcg aagccaaaca cgtcatcgta
      661 gccaccggtt ccgtaccgcg tccgctgcca caagtcgcta tcgataacgt gaacgtattg
      721 gacaacgaag gcgcattgaa cctgaccgaa gtacctgcca aactcggcgt gatcggttcc
      781 ggcgtgattg gtttggaaat gggttccgta tggaaccgcg tgggtgcaga agttaccatt
      841 cttgaagctg cgccgacctt cttggctgcc gccgaccaac aaatcgccaa agaagccttc
      901 aaatacttca ccaaagagca aggcttgagc atcgaattgg gtgtaaaaat cggtgacatt
      961 aaatctgaag gcaaaggcgt ttccgttgct tacgaaactg ctgccggcga agccaaaacc
     1021 gaagtattcg acaaactgat cgttgccatc ggccgtattc caaacaccaa aggcctgaac
     1081 gcggaagccg taggcttgga aaaagacgag cgcggcttta tcaaagtgga cggcgaatgc
     1141 cgtaccaacc tgcctaacgt atgggcaatc ggcgacgtgg ttcgcggccc gatgttggca
     1201 cacaaagcca gcgacgaagg cgttgccgtt gccgagcgca ttgccggtca aaaaccgcat
     1261 atcgacttca acaacgtacc gttcgtgatt tacaccgacc ctgaaatcgc ttgggtgggt
     1321 aaaaccgaag agcagctcaa agccgaaggc gtggagtaca aaaaaggtac ttcaggtttc
     1381 ggtgcaaacg gtcgcgcatt ggcaatgggc aaagctaaag gtacggttaa agtattggca
     1441 gataccaaaa ccgaccgcat cttgggcgta cacatgattg gcccggttgt cagcgaattg
     1501 gttaccgaag gcgtgactgc gctcgaattc ttcgccagca gcgaagacat cgcccgcatt
     1561 atccatgccc acccaacctt gtccgaagtg gttcacgaag ctgcattggc ggccgacaaa
     1621 cgcgctttgc acggttgata gacattaagg ccgtctgaaa tttttcagac gaccttaatg
     1681 ccttcgacaa attgaatgtt ccgagagctc cgttttctga tttataattc cgtcagacaa
     1741 acaaacagca tttacattcg ttatgaacaa agaaatagtc ggtattttct ttataccgat
     1801 gggcatcatc agcatgtgta tggccgcatt gtggcagatg tatgtgatga tgaccgaaac
     1861 ttatacgctc aaccgtttca aagataaaga attggtttgg cgcgtggcat tgttgtttat
     1921 cagtttcagc cttgccgttt atctgctctg tccgaattcg cgtaaaaaag gcatcgtctt
     1981 ttttattctc gggggaggcg gtgcagccat gtatctgctg gcgcggatgt ggttgccttt
     2041 cagcaagtga aacgacgatt ttccgaccgc cgaaaggtag tctgaaacgc acgggcttgc
     2101 catttggagg caaactcggg gcattccact aatctaaagg agaaatccat gaatttacac
     2161 gagtatcagg ctaaagaact gctggctagc tacggtttgc ccgtacaagg cggtattttg
     2221 gcacacaacg gcgaagaagc cgctgcagct tacgacaaat tgggcggcaa attcgctgtt
     2281 gtcaaagcac aagtacatgc cggcggccgc ggtaaagcgg gcggcgtaaa agtcgttaaa
     2341 agccgcgaag aagctaaaga agtggctgaa agcctgattg gcaccaactt ggtaacttac
     2401 caaaccgatg ccaacggtca acctgtcaac agcgttttgg tttgcgaaga tatgtatccg
     2461 gttcaaaccg agctgtactt gggcgcagtg gttgaccgtt ctacccgccg cgttacattc
     2521 atggcttcta ccgaaggcgg tgtggaaatc gaaaaagttg ctgccgaaac tccagaaaaa
     2581 atcttcaaag taaccgttga tccgctggtc ggcctgcaac cttgccaagc tcgcgaagtt
     2641 gccttccaac tgggcttgaa agacaaacaa atcaacgagt tcgtcaaact gatgaccggc
     2701 gcgtacaaag cgtttgtcga aaacgacttc gccctgtttg aagtcaaccc gctggcagtt
     2761 cgcgaaaacg gcgcactggc ttgcgtggac ggcaaaatcg gcatcgacag caacgcgctc
     2821 taccgcctgc caaaaatcgc tgaattgcgc gacaaatctc aagaaaacga acgtgaactg
     2881 aaagcttctg aattcgacct gaactatgtt gccctagaag gtaatatcgg ctgtatggtt
     2941 aacggtgccg gtttggcgat ggccaccatg gacatcatca aactgaaagg cggccaacct
     3001 gccaacttct tggacgttgg cggcggcgca accaaagacc gcgtggttga agcgttcaaa
     3061 ctgattttgg aagacaaatc cgttaaaggc gtattgatca acatcttcgg cggtatcgta
     3121 cgttgcgaca tgattgcgga agccatcgtg gcagccgtta aagaaatcaa cgtcgacgtt
     3181 cctgtcgttg ttcgtttgga aggtaacaac gccgaactcg gcgcgaaaat cctgaacgaa
     3241 tcaggcctga aactgacttc tgcagacggc ctgaatgacg cagccgaaaa aattgttgca
     3301 gccgtaaacg cctaaggaga aaagaatgag cgtattgatt aataaagaca ccaaagtatt
     3361 ggttcaaggt ttcaccggta aaaacggtac tttccactcc gaacaagctc tggcttacgg
     3421 cactaaagtt gtcggcggcg ttaccccggg caaaggcggt caaacccacc tgaacctgcc
     3481 tgtattcaac accatgaaag aagctgttaa agaaactggc gcggatgcat ccgtaattta
     3541 cgttcccgct ccgtttgtgt tggattctat cgttgaagcc gttgattcag gcgtaggctt
     3601 ggtcgttgtg attaccgaag gcgtacctac tttggacatg ctcaaagcca aacgttactt
     3661 ggaaaccaac ggcaacggta cccgcttggt cggccctaac tgcccgggcg tgattactcc
     3721 gggcgagtgc aaaatcggca ttatgccggg ccacatccac actccgggcc gcatcggtat
     3781 tatttcccgt tccggtacat tgacttacga agccgtggca caaaccacca aactgggctt
     3841 gggtcaatca acctgtatcg gtatcggcgg cgacccgatt cctggtatga accaaatcga
     3901 cgcactgaaa ctcttccaag aagacccaga taccgacgcc atcatcatga ttggtgaaat
     3961 cggcggtact gcggaagaag aagcagccga atacatccaa tccaacgtaa ccaaacctgt
     4021 tgtaggttac attgctggtg ttaccgctcc taaaggcaaa cgtatgggcc acgccggtgc
     4081 gattatttcc ggcggtaaag gtactgccga agaaaaattc gccgctttcg aaaaagccgg
     4141 tatcgcctat actcgcagcc ctgccgagtt gggcacgacg atgctggaag tgttgaaggc
     4201 aaaaggtttg gcataatcag gtttgacaac tgattaaaca tcaaatgccg tctgaaaccg
     4261 gaaatcgggt ttcagacggc attttgtttg tcatttcaaa aagaggcagc ctcaacatac
     4321 ccacattatt ttttcccttt tggggcagtc agagaccttt gcaaaattcc ccaaaatccc
     4381 ctaaattccc accaagacat ttaggggatt ttggggaatt ttgcaaaggt ctcgggctaa
     4441 gtgtgcctgt ttgcgcctaa aaggcggccc ggatgcctga ttatcgggta tcctgggagg
     4501 attaaggggg tattggggta aaattagtgg atatttgaaa cgaaaacagc cgaaaacctg
     4561 tgtttgggtt tcggctgtcg ggagggaaag gaattttgca aagctctcgt attggctttg
     4621 aagttccgtg taattcacag gtagggcgtg tggcacagcc acgcacgcgg tcggttgggt
     4681 atgcaggcta cggctttctc tgttgaaact gcaacacgtc agctaccaac cgtgtgcgta
     4741 gcgcacacgg ttggttggga tacaagttac tgtttgttct taaatggagt accaacatca
     4801 agggcttttg ataatcctga aaatattaaa tattcagttt cagtttttat tttaggagaa
     4861 atatttgcat aatttctatc tttaaagcac caatggatat atggtttcgt ttcatcttct
     4921 gggttatcta aataagcaat aacattcaag tcaaataaaa attgcaaaaa ctcattagca
     4981 gtactcataa atttaggtat ttccactgat gttgtttgta agtgcttttt caaacgttca
     5041 aatgctttta aaaaatcact atatttaaat ctatctttcc cgtttaaaaa ttcaaaaaat
     5101 ttcaggaaat tttgataatc actttgacta taataaaaca aaagatgatc tttgatttca
     5161 ccaagtaaat atatcgagta ttctctttga aaagaagtat tatcaaaatc ttctgctacg
     5221 acataatctt ccttactttt cttatttttt tgtagcaaag taagcatctg aagaatatcg
     5281 cgaggtcgat aatacgattt tcttaggaag ctaataaatg aagttaaatt tttatactca
     5341 tcatgtaaat taggagcatt ccatggaaaa taataatccc atgagttgcc tttttctaaa
     5401 ctatcttgtt tttcttgctg ggttctcaaa agatgatcaa aaacgccaaa aatctttgaa
     5461 cttctataag atttataatc cgtcctccag tctaaaaata ctgaattatc ttgaagtttg
     5521 gtattttgat tttgtaaacc taatgaatca aagatatcag gtctaatcaa taacacaact
     5581 ctcatccttc ccttactatc tttaatggaa gggaagatat cattatttaa catccatatg
     5641 gcgttagcaa gaccttttac acactcatga tattcatcaa atggaatctg tgatggtcta
     5701 atatctatcc catcaataaa caaaatatga ttatctttta gctttaactg agataaagca
     5761 tctttaaatt ttctttcaat aaaacctaaa tttgcttgga atttactttc tgtaaaagtt
     5821 atttgttggg attcctcttc acctagttta acaaattttc caaaaatcat ttccgcagct
     5881 tcttttgaat tttctattaa agttattgct tgtacaattt ccggatcaaa agcgccataa
     5941 taatattcat ttatagcctc atctaaggct ttaaatttat taaatattga agataatatt
     6001 ccgttttctt tacatttgat ttgatttgat atcaacagat ataaaatgac tttccaaata
     6061 cttgtaaaat ctgaaacagt taagtgtctt gctttcttta gctgaataaa ttttgaataa
     6121 tcggtttcac gaacaaactt agtagtggca tgtatgtttt tatagaagtt attagttaaa
     6181 taaacagcat atgctgtctt tccagttccc ttttctccga ttaaaaacga aatatttggt
     6241 tcacataatt catccaaata ttctcctttt acaaatattc ggttaaataa atctttattt
     6301 tctcttcttc tgtagtttgc agcatccaca aatccaaatt ctaatgtttt taacggtttc
     6361 atcttaataa tctcctattt aattttgaat taaacttacc tcaaaaccac cttcaaatac
     6421 ttcccagtat aactcccctt aactttcgcc acctgctcag ggctaccaca agcaataatc
     6481 ctgcccccgc catcgccgcc ttccggccct aagtccacaa tccaatcggc ggttttaatc
     6541 acgtctagat tatgctcgat aatcacaatc gagttgcctt tgcctttcag acgacctatg
     6601 acttccagca gcagggcgat gtcggcgaag tgtaggccgg tggtgggttc gtcgaggatg
     6661 tagagtgttc tgccggtgtc gcgtttggag agttccaagg cgagtttgac gcgctgggct
     6721 tcgccgccgg agagggtggt ggcggattgg ccgaggcgga tgtagccgag gcctacgtcc
     6781 atcagggttt gcagtttgcg cgatacggtg gggacggcgt cgaaaaattc gcgggcttct
     6841 tcgacggtca tgtcgaggac ttggctgatg tttttgcctt tgtattggat ttcgagcgtt
     6901 tcgcggttgt agcgtttgcc gtggcagact tcgcagggga cgtacacgtc gggcaggaag
     6961 tgcatttcga ctttaatcac gccgtcgcct tggcaggctt cgcagcggcc gcctttgacg
     7021 ttgaaggaga atctgccgac gttgtagccg cgttcgcgcg agaggggtac gccggcgaag
     7081 agttcgcgga tgggggtgaa caggccggtg taggtggcgg ggttggagcg cggggtgcgg
     7141 ccgatggggg attggtcgac gttgatgact ttgtcgaggt gttcgaggcc gcggatgtcg
     7201 tcgtatgggg cgggttcttc ttgggctcgg ttgagttcgc gggcggtgat tttggcgagg
     7261 gtgtcgttaa tcagggtgga tttgccgctg ccggatacgc cggtgatgca ggtaatcaaa
     7321 ccgagcggca gttcgagggt aacgtttttg aggttgttgc cgcgcgcgcc tttgaggacg
     7381 agcattcggc cgggattgac gggcgtgcgt tcagacggca cggcaatgga ttttttgccg
     7441 ctgaggtatt gtccggtaac ggagttttcg cattgggcga cgttttcggg cgtgtcggca
     7501 atcagtacgt tgccgccgtg ttcgcctgcg ccggagccca tatcgacgac gaaatcggct
     7561 tcgcggatgg cgtcttcgtc gtgttcgacc acaatcacgc tgttgcccaa atcgcgcagg
     7621 cgtttgaggg tggcaagcag gcggtcgttg tcgcgctggt gcaggccgat ggagggttcg
     7681 tccaaaacgt acatcacgcc ggtcaggccg ctgccgattt ggctggcaag gcggatgcgc
     7741 tgggcttcgc cgccggaaag ggtttcggcg gagcgggaga gattcaggta atccagcccg
     7801 acgttaatca aaaagccgag ccgctcggtg atttctttga ggattttttc ggcgatttgt
     7861 tttttgttgc cgtccaaatc cagcgtttca aagaatcggt gggttttggt cagcggccag
     7921 gcggagactt cgtgtaaagg ttcgccgctg acgtaaacgt agcgcgcttc tttgcgcaaa
     7981 cgtgcaccgc cgcagctcgg gcaggcgcgg tggttttggt attcgcgcaa tttttcgcgc
     8041 acggtttcgc tgtcggtttc gcggtagcgg cgttcgagat tgggaatgat gccttcgaag
     8101 gcgtggctgc ggttgaaggt ggtgccgcgt tcggataggt aggtgaaatc aatgacttcc
     8161 ttgcctgagc cgtgcagcac gacttttttc actttttcag gtagcgtttc ccaagcagcc
     8221 tgcacatcaa aaccgtaatg ccgcgccagc gactgaatca tttggaaata gaactggttg
     8281 cgcttgtccc agccgtcaat cgcgcccgtt gccagcgaca attcgggatg ggcgactact
     8341 ttttcggggt cgaagaaatt ggtgttgccc aagccgtcgc aagtcgggca ggagcccatc
     8401 gggttgttga acgaaaagag gcgcggctcc aattcgggca ggctgtacga acatacgggg
     8461 caggcgaaac gcgcggaaaa ccaatgctct tcgccgctgt ccatttccat cgccagcgcg
     8521 cgctcgttgc cgtggcgcag cgcggtttca aaactttccg ccagccgctg cttgatgttc
     8581 gccttcactt tcacgcggtc gatgaccacg tcgatattgt gtttgatgtt tttttccagc
     8641 ttcggcactt cgtcaagctg atagacctcg ccgtccacgc gcacacgggc aaagccttgc
     8701 gcctgcaagt cagcaaagaa gtcgacaaac tcgcccttac gctcacgaac tgccggcgca
     8761 agaatcatca cgcgcgtgtc ttccggcagt tttaatacgg catcgaccat ctgagatacg
     8821 gtttggctgg atagcggcag attgtgttcg gggcaatacg gcgtgccgac gcgtgcgtac
     8881 aaaaggcgca ggtaatcgtg gatttccgta accgtgccga cggtggaacg cggattgtgg
     8941 ctggtggatt tctgctcgat ggaaatcgcg ggcgacagac cctcgatcaa atcgacatcg
     9001 ggtttgtcca tcatctgcaa aaactgccgc gcataggcgg aaaggctctc gacataacgc
     9061 cgctgccctt cggcatacag cgtgtcaaac gccagcgacg acttgccgct gcccgacaat
     9121 cctgttacca ccacgagctt gtggcgcgga atgtccaaat cgatgttttt caaattatgc
     9181 gtgcgcgcgc cgcggatgcg gatggtgtcg ttgtcgtgcg aatgttgggg atgatgcttg
     9241 cacataatgg atgccgcctg aaaaataaag gaaaactggt attgtagcac tttctcggat
     9301 gccgtctgaa gccgcgttca gacggcattt gtcggcggag tacggcagat tccgctataa
     9361 tgtcggcaat tttaacccgc ttgaacaaaa ggatgacaaa tgaaccgtct ttacccccac
     9421 ccgattatcg cccgtgaggg ctggccgatt attggcggcg gtttggcttt gagcctgctg
     9481 gtgtcgatgt gttgcggctg gtggtctttg ccgttttggg tgtttaccgt atttgccctg
     9541 cagtttttcc gcgaccctgc gcgtgagatt ccgcaaaatc ctgaagcggt gttgagccct
     9601 gttgacggcc gcattgtggt ggtcgaacgc gcacgcgatc cgtatcgtga tgtcgatgct
     9661 ttgaaaatca gtatttttat gaacgtgttc aacgtgcatt cgcaaaaatc gcctgccgat
     9721 tgtacggtaa cgaaagtggt ctataacaaa ggcaaattcg tgaatgcgga tttggacaaa
     9781 gccagcacgg aaaacgaacg taatgcggtg ttggcgacta cggcttcagg tcgtgaaatt
     9841 acttttgttc aagtggccgg tttggtggcg cgccgtattt tgtgctacac ccaagcaggt
     9901 gcgaaactgt cccgcggcga acgctatggc tttattcgct tcggttcgcg cgtggatatg
     9961 tatctgccgg ttgacgcgca ggcgcaagtg gcgattggcg ataaagtaac cggcgtaaaa
    10021 actgtattgg cgcgtctgcc gctgactgcg ccgcaaatcg aatccgagcc tgaatctgag
    10081 ccagcttcac aagcggcttc ggttgaaaca gcggcaaacc catctgccga acaacggcaa
    10141 attgaagcaa cggcggctaa gattcaagcg gctgtgcaag atgtgttgaa agattaattt
    10201 tgcgaactaa aatagaaaat atcagtacca tcattcacac gaatgaggaa gtttggtttt
    10261 ttgaattttt gctaatgttc acaccgtcat tcccacgaaa gtgggaatct agaaacttaa
    10321 cgttacgacg atttatcgga aacgactgaa accggacgga ctggattccc gcctgcgcgg
    10381 gaatgacggc acattagtta cctaaaactt aaaaaaaaaa cagaaacctt tccgcctcat
    10441 tcccacgaaa gtgggaatct agaacccaaa tgctaaggcg atttatcgga aacggctgaa
    10501 accgaagaga c
//
LOCUS       DAXHSF010000056        10465 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.056, whole genome shotgun sequence.
ACCESSION   DAXHSF010000056 DAXHSF010000000
VERSION     DAXHSF010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10465
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.056"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            83..1048
                     /locus_tag="ACFM8S_001048"
     CDS             83..1048
                     /locus_tag="ACFM8S_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6314130.1"
                     /translation="MLRLTALTLCTVLALGACSPKDSDSAPQAKEQAVSAAQSESASV
                     TVKTARGDVQIPQNPERIAVYDLGMLDTLNKLGVKTGLSIDKNRLPYLDEYYKTTKPA
                     GTLFEPDYEALNAYKPQLIIIGSRAAKAFDKLNAIAPTIEMTADTANLKESSKERIDA
                     LAQIFGKQAEADKLKAEIDASFEAAKTAAQGKGKGLVILVNGGKMSAFGPSSRLGGWL
                     HKDIGVPAVDESIKEGSHGQPISFEYLKEKNPDWLFVLDRSAAIGEEGQAAKDVLNNP
                     LVAETTAWKKGQVVYLVPETYLAAGGAQELLNASKQVADAFNAAK"
     gene            1066..1194
                     /locus_tag="ACFM8S_001049"
     CDS             1066..1194
                     /locus_tag="ACFM8S_001049"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314131.1"
                     /translation="MPSETRMQTASWVSDGICPIRRFQTRLSAPTVRTALFESSTP"
     gene            1213..2181
                     /locus_tag="ACFM8S_001050"
     CDS             1213..2181
                     /locus_tag="ACFM8S_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC6314132.1"
                     /translation="MTAKPFSLNLTNLLLLAVLFAVSLSVGVADFRWSDVFSLSDSQQ
                     VMFISRLPRTFAIVLTGASMAVAGMIMQILMRNRFVEPSMVGASQSAALGLLLMTLLL
                     PAAPLLAKMSVAAVAALIGMLVFMLLIRRLPPTAQLMVPLVGIIFGGVIEAVATFIAY
                     ENEMLQMLGVWQQGDFSGVLLGRYELLWATGILALFAYLIADQLTILGLGETVSVNLG
                     LNRTAILWSGLIIVALITSLVVVTVGNIPFIGLVVPNIISRLMGDKLRQSLPAVALLG
                     ASLVLLCDIVGRVIVFPFEIPVSTVFGVMGTALFLWLLLRKPAHAV"
     gene            2171..3145
                     /locus_tag="ACFM8S_001051"
     CDS             2171..3145
                     /locus_tag="ACFM8S_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFC6314133.1"
                     /translation="MPSEKNIGFMAGSSRPLWVAFALLLVSCILFMTLNVKGDWDFVL
                     HLRLTKLAALLMVAYAVGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTFGGV
                     GYASLPLTGKFGFELVVMMGGSLLLFYTLIKQGGRDLSRMILIGVIFGILFRSLSSLL
                     SRMIDPEEFTAAQANMFAGFNTVHSELLGIGALILLVSAAAVWRERYRLDVHLLGRDQ
                     AVNLGISYTRNTLWILLWIAALVATATAVVGPVSFFGLLAASLANHFSPSVRHSVRLP
                     MTVCVGGILLVGGQTVFEHLLGMQAVLSVVVEFAGGLVFLYLVLKHKK"
     gene            3190..3816
                     /locus_tag="ACFM8S_001052"
     CDS             3190..3816
                     /locus_tag="ACFM8S_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HFC6314134.1"
                     /translation="MAQEHFPSFFNQAPTITVRDALAEFLGAAENGILTYRYADAVRL
                     CGHSCPTVAGAYLMVIKGLKALYGAELPQRGDIEAFMQGGRDEGTTGVTASVVQLLTG
                     AAPETGFGGVGPAGRFARRHLLSFGAGEINGTLALRRRDTGKTVAVSLNAALQPFAPE
                     MRELMPKAVGGSASAEELERFGQLWQARVKAFLTESADDPQFVIVREV"
     gene            3898..4656
                     /locus_tag="ACFM8S_001053"
     CDS             3898..4656
                     /locus_tag="ACFM8S_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC6314135.1"
                     /translation="MITIRNVSYRIGTRPILDNVSLDIPEGGITALVGPNGAGKSTLF
                     SFMARLRPLESGSIAYRGKNLAGTPTAELARTLSILTQENSIMSRITVRDLLMFGRYP
                     YHQGRPTAECRRIVNGAIEEFHLQDLSDRYLTELSGGQRQRAMIAMVFCQSTDYVLLD
                     EPLNNLDMYHARSLMQILQKLTREHKRTTVAVLHDINQAAAYADHVVAMKNGKAVLTG
                     TPEEVFTVHNIKELFDMDVDVLDYEGKKLIVHHI"
     gene            complement(4715..5053)
                     /locus_tag="ACFM8S_001054"
     CDS             complement(4715..5053)
                     /locus_tag="ACFM8S_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFC6314136.1"
                     /translation="MKKIEAIVKPFKLDDVREALTEIGITGMTVSEVKGFGRQKGHTE
                     IYRGAEYAVDFLPKVKIELVLADDAVERAIDVIVEVARSGKIGDGKIFVLPVEEAIRI
                     RTGERSDAAV"
     gene            5148..5360
                     /locus_tag="ACFM8S_001055"
     CDS             5148..5360
                     /locus_tag="ACFM8S_001055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314137.1"
                     /translation="MPETVIPAQAGIPISAFQQYLKVSVNPNFHIPNRAETTAFDLKT
                     YGSSCPPLYFLQLSAYPALGKNQKNR"
     gene            5517..9473
                     /gene="purL"
                     /locus_tag="ACFM8S_001056"
     CDS             5517..9473
                     /gene="purL"
                     /locus_tag="ACFM8S_001056"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFC6314138.1"
                     /translation="MSVVLPLRGVTALSDFRVEKLLQKAAALGLPEVKLSSEFWYFVG
                     SEKTLDAATVEKLQALLAAQSVEQTPEAREGLHLFLVTPRLGTISPWASKATNIAENC
                     GLAGIERIERGMAVWLEGALTDEQKQQWAALLHDRMTESVLPDFQTASKLFHHLESET
                     FSTVDVLGGGKEALVKANTEMGLALSADEIDYLVENYQALQRNPSDVELMMFAQANSE
                     HCRHKIFNADFILNGEKQPKSLFGMIRDTHNAHPEGTVVAYKDNSSVIEGAKIERFYP
                     DAAENQGYRFHEEDTHIIMKVETHNHPTAIAPFAGAATGAGGEIRDEGATGKGSRPKA
                     GLTGFTVSNLNIPGLKQPWEQDYGKPEHISSPLDIMIEGPIGGAAFNNEFGRPNLLGY
                     FRTFEEKFDGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGMLIGLGG
                     GAASSMDTGTNDASLDFNSVQRGNPEIERRAQEVIDRCWQLGGKNPIISIHDVGAGGL
                     SNAFPELVNDAGRGAVFKLREVPLEEHGLTPLQIWCNESQERYVLSILEKDLDTFRAI
                     CERERCPFAVVGTATDDGHLKVRDDLFANNPVDLPLNVLLGKPPKTTRTDKTVTPSKK
                     PFHADDIDITEAAYRVLRLPAVAAKNFLITIGDRSVGGMTHRDQMVGKYQTPVADCAV
                     TMMGFNTYRGEAMSMGEKPTVALFDAPASGRMCVGEAITNIAAANIGDIGNIKLSANW
                     MAACGNEGEDEKLYRTVEAVSKACQALDLSIPVGKDSLSMKTVWQDGEEKKSVVSPLS
                     LIISAFAPVEDVRKTVTPELKNVEDSVLLFVDLGFGKARMGGSAFGQVYNNMSGDAPD
                     LDDTGRLKAFYNVIQQLVAEDKLLAYHDRSDGGLFATLAEMAFAARCGISADIDCLMD
                     KFLPIHHPDFQGDPAEDLSDELYNHAAIKILFNEELGAVIQIRQQDRDYVDAAFKAAD
                     LTDAVSRIGSPDFDNEFISFFGYGYFLEQNRADLQRAWQETSHAIQRLRDNPACADSE
                     FALIGDNGRSALFANLKFDVNEDIAAPFINSGAKPKIAILREQGVNGQIEMAAAFTRA
                     GFDAYDVHMSDLMAGRIHLADFKMLAACGGFSYGDVLGAGEGWAKSILFHPALRDQFA
                     AFFADPDTLTLGVCNGCQMVSNLAEIIPGTAGWPKFKRNLSEQFEARLSMVHVPKSAS
                     LILNEMQGSSLPVVVSHGEGRADFALHGGNISADLGIALQYVDGQNQVTQTYPLNPNG
                     SPQGIAGITNADGRVTIMMPHPERVYRAAQMSWKPEGWTELSGWYRLFAGARKALG"
     gene            9568..10326
                     /gene="gloB"
                     /locus_tag="ACFM8S_001057"
     CDS             9568..10326
                     /gene="gloB"
                     /locus_tag="ACFM8S_001057"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFC6314139.1"
                     /translation="MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLM
                     LAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIEAATHTVTAGTQFTFGEGLITVW
                     ATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTIEQLYDSFQRFNQLPEGT
                     LFYPAHEYTAANLRFAAHIEPDNADIQTALKAAEHTPTLPVTLAHERRVNPFLRVDLP
                     HVRDRAEALSGKTLNSSLDTFVALRELKNQYRTK"
BASE COUNT         2378 a         3139 c         2697 g         2251 t
ORIGIN      
        1 ttatcattta tccttttcta aagccgttcc ggtttgtccg accggcggct ttgccccaat
       61 atccccattt tggagacacc ctatgttgcg tttgaccgca ttgaccctct gcaccgtcct
      121 cgccttgggc gcgtgttcgc cgaaagattc cgactctgcc ccacaagcca aagaacaggc
      181 agtttccgcc gcacaatccg aaagcgcgtc cgttaccgtc aaaaccgcgc gcggcgatgt
      241 tcaaataccg caaaaccccg aacgtatcgc cgtttacgat ttgggtatgc tcgacacttt
      301 aaacaaattg ggcgtgaaaa ccggtttatc catcgataaa aaccggctgc cttatttgga
      361 cgaatattac aaaaccacga aacctgccgg aactttgttc gagccggatt acgaagcctt
      421 aaacgcctac aagccgcagc tcatcatcat cggcagccgc gcagccaaag cgtttgacaa
      481 gttgaacgcc attgcgccga ccatcgagat gaccgccgat accgccaacc tgaaagaaag
      541 ctcaaaagag cgtatcgacg cgctggcgca aatcttcggc aaacaggcgg aagccgacaa
      601 gctgaaggcg gaaatcgacg cgtcttttga agccgcgaaa accgctgcgc aaggcaaagg
      661 caaaggtttg gtgattttgg tcaacggcgg caagatgtcc gccttcggcc cgtcttcacg
      721 actgggcggc tggctgcaca aagacatcgg cgttcccgct gtcgatgaat cgattaaaga
      781 aggcagccac ggtcagccta tcagctttga atacctgaaa gagaaaaatc ccgactggct
      841 gtttgtttta gaccgcagcg cggccatcgg cgaagaaggt caggcggcga aagacgtgtt
      901 gaataatccg ctggttgccg aaacaaccgc ctggaaaaaa ggacaggtcg tgtacctcgt
      961 tcctgaaact tatttggcgg ccggcggcgc gcaagagctg ctgaatgcaa gcaaacaggt
     1021 tgcggatgct tttaacgccg caaaataatg aaacggcggc attcgatgcc gtctgaaaca
     1081 cggatgcaaa ccgcctcctg ggtttcagac ggcatttgcc cgatacggag gtttcaaaca
     1141 aggctttccg ctccgacggt tcggactgcc ttgtttgaat cttctacgcc ttaacgcttt
     1201 tcccttctgt ttatgactgc caaacctttt tccctcaacc tgaccaacct cctgctgctg
     1261 gcggtgttgt ttgccgtcag cctgtcggtc ggcgttgccg atttccgctg gtcggatgtg
     1321 ttttcgctat ccgacagcca gcaggttatg ttcatcagcc gcctgccgcg cacgtttgcg
     1381 attgtgctga cgggcgcgtc gatggcagtg gccggcatga ttatgcagat tttgatgcgc
     1441 aaccgttttg tcgaaccgtc gatggtgggc gcaagccaaa gcgcggcttt gggtttgctg
     1501 ttgatgaccc tgctgctgcc ggccgcgccg ctgctggcaa aaatgtcggt tgccgccgtt
     1561 gccgcgctga tcgggatgtt ggtctttatg ttgctgatcc gccgcctgcc gccgaccgcg
     1621 caactgatgg tgcctttggt cgggattatt ttcggcggtg tgattgaggc ggtggccacc
     1681 tttatcgcgt atgaaaacga aatgctgcaa atgctcggcg tgtggcagca gggcgatttt
     1741 tccggcgtgt tgctcggacg ctatgagctg ttgtgggcaa cgggaatttt ggctttgttt
     1801 gcctatttga ttgccgacca actgacgatt ttgggtttgg gtgaaacggt gagcgtgaac
     1861 ttggggctga accggacggc gattctgtgg tcggggctga ttattgtggc gctgattacg
     1921 tctttagtgg tcgtgacggt cggcaatatt ccgtttatcg gcctagtcgt gccgaacatc
     1981 atcagccgcc tgatgggcga caagttgcgt caaagcctgc ctgcggtggc cttgctgggc
     2041 gcatctttgg tgttgctgtg cgatattgtc ggacgcgtga ttgtgtttcc gtttgaaatt
     2101 ccggtctcta cggtttttgg tgtgatgggg acggctttgt ttttgtggct tttattaagg
     2161 aaacctgctc atgccgtctg aaaaaaatat cggttttatg gcaggaagca gccgtccgtt
     2221 gtgggttgcc tttgcgctgt tgctggtttc ctgcatcttg tttatgacgc tcaacgtcaa
     2281 aggcgattgg gattttgtct tgcacctgcg cctgaccaaa cttgccgcgc tgctgatggt
     2341 cgcctatgcg gtcggcgtgt ccacgcaact cttccaaacg ctgaccaaca atccgattct
     2401 gaccccttcg attttgggtt tcgattcgct atatgtgttt ttgcagacct tgctggtgtt
     2461 tacgttcggc ggcgtgggct atgcttccct gccgttgacg ggcaaattcg gctttgaact
     2521 ggttgttatg atgggcggtt cgctgctgtt gttctacacg cttatcaaac agggcggacg
     2581 agatttgtcg cgcatgattt taatcggcgt gattttcggg attttgttcc gcagcctgtc
     2641 gtcgttgctt tcgcgcatga ttgaccccga agaatttacc gccgcgcagg cgaatatgtt
     2701 tgccggattc aataccgtcc acagcgagct tttgggcata ggcgcgctga ttctgctcgt
     2761 cagcgcggca gcggtttggc gcgaacgcta ccgcttggac gtacaccttt tggggcgcga
     2821 ccaagccgtc aatttgggca tcagctacac gcgcaacacc ttgtggatac tgctttggat
     2881 tgccgcgctg gtggcgacgg cgaccgccgt ggtcggcccg gtaagctttt tcgggcttct
     2941 cgccgcctcg cttgccaacc acttttcccc gtccgtgcgc cattccgtcc gcctgccgat
     3001 gacggtttgt gtcggcggta ttcttctggt cggcggacag accgtattcg aacacctgct
     3061 cggtatgcag gcagtgttga gcgtggtagt agaatttgcg ggcggactcg ttttcctcta
     3121 tctcgtttta aaacacaaaa aatgacggat gccgtctgaa cggccgtccg cctcgacagg
     3181 acaaaacata tggcacaaga acatttccca tcattcttca accaagcccc gaccatcacc
     3241 gtcagggacg cgcttgccga attcctcggc gcggccgaaa acggcatcct cacttaccgc
     3301 tacgccgatg ccgtgcgcct gtgcggacat tcctgcccga ccgtcgcggg cgcgtacctg
     3361 atggttatca aaggtctgaa agccctttac ggcgcagagc tgccgcaacg cggcgacatc
     3421 gaagccttta tgcagggcgg gcgagacgaa ggcacgaccg gcgttaccgc ttccgtcgtc
     3481 caactcctga ccggcgcggc acccgaaacc ggctttggcg gcgtaggccc ggccggacgc
     3541 tttgcccgcc gccacctctt gtcctttggt gcaggcgaaa tcaacggcac actcgcgctc
     3601 cgccgccgcg ataccggcaa aaccgttgcc gtcagcctca acgccgccct gcaacccttt
     3661 gcacccgaaa tgcgcgaact tatgcccaaa gccgtcggcg gcagcgcaag cgcagaagaa
     3721 ctcgaacgct tcggacaact ctggcaggcg cgcgttaaag catttttgac cgaatcggcg
     3781 gacgacccgc agttcgtcat cgtccgcgaa gtgtgagcgt tcagacggca ttccgaattt
     3841 caaatgccgt ctgaaccccc gccaaacaac aaacctacgc ccgacaagca tccgcctatg
     3901 attaccatcc gcaacgtcag ctaccgcatc ggcacacgcc ccatcctcga caacgtcagc
     3961 ctcgacatcc ccgaaggcgg cattaccgcc ctcgtcggcc ccaacggtgc gggcaaatcc
     4021 accctgtttt cctttatggc gcggctgcgc ccgcttgaaa gcggcagcat cgcctaccgc
     4081 ggcaaaaacc ttgccggcac ccccaccgcc gaactcgccc gcacgctctc catcctcacc
     4141 caagaaaaca gcatcatgag ccgcatcacc gtgcgcgacc tgctgatgtt cggccgttac
     4201 ccctaccatc aaggcagacc gactgccgaa tgccgccgta tcgttaacgg tgcaatcgaa
     4261 gaattccacc tgcaagacct ctccgaccgc tatctgaccg agctctccgg cggccaacgc
     4321 caacgcgcca tgattgcgat ggtgttctgc caaagcaccg actacgtcct tttggacgaa
     4381 ccgctgaaca acctcgatat gtatcacgcc cgctcgctca tgcagattct gcaaaagctc
     4441 acgcgcgaac acaaacgcac caccgtcgcc gtattgcacg acattaacca ggccgcagcc
     4501 tacgccgacc acgtcgtcgc catgaaaaac ggcaaagccg tcctgaccgg cacgcccgaa
     4561 gaagtgttca ccgtacacaa catcaaagaa ctgttcgaca tggatgtgga cgtactcgac
     4621 tacgaaggca aaaaactcat cgtccaccat atctgaagct caaaaagaaa atgccgtctg
     4681 aaaacctttc agacggcatt tccattccaa acgctcaaac cgccgcgtcc gaacgttcgc
     4741 ccgtgcggat acggattgcc tcctcgaccg gcagcacaaa aatcttgccg tcgccgattt
     4801 tgcccgaacg cgccacctcg acaatcacgt caatcgcgcg ttccacagcg tcatccgcca
     4861 acaccagctc gattttgacc ttgggcagga aatcgacggc gtattccgca ccgcgataga
     4921 tttccgtatg ccccttctgc ctgccgaacc ctttgacctc gctgacggtc atccccgtaa
     4981 tgccgatttc cgtcaacgcc tcgcgcacgt cgtcgagttt gaacggtttg acaatcgcct
     5041 cgattttttt catcacattc tccattaatt aacgtaaaca aacgacaaat atagcagaaa
     5101 ccgccggcaa acgataaacg gtactgttcg cccggcgatg cggcgatttg ccggaaaccg
     5161 tcattcccgc gcaggcggga atcccgatct ctgcatttca gcaatattta aaggtttctg
     5221 taaatccaaa cttccatatt cctaaccgtg cggaaacgac ggcatttgat ctaaaaacat
     5281 acggaagcag ttgcccgccc ctctacttcc tgcaattatc cgcatacccg gctttgggta
     5341 aaaatcagaa aaacagataa aacccttatt ccttaatatc ttcagacggc acctgtattt
     5401 tccccaactg tatgccgtct gaagattgtc aacattttaa gccaaaatat ccaagcaata
     5461 caaacctgtc cggcgtataa tccctctttt tcccaaccgc atttgagagc cgaattatgt
     5521 ctgttgtgtt gcccttgcgc ggcgttaccg ccctttccga tttccgtgtt gaaaaactct
     5581 tgcaaaaagc cgccgcactc ggtctgcccg aagtcaaatt aagcagcgaa ttttggtatt
     5641 tcgtcggcag cgagaaaaca ctggatgccg caactgtcga aaaactgcaa gccttactgg
     5701 cagcgcaaag cgttgaacaa acgcccgaag cgcgcgaggg cttgcattta tttttggtca
     5761 cgccccgttt gggtacgatt tcgccttggg cttccaaggc aaccaatatc gcggaaaact
     5821 gcggtttggc aggcatcgag cgcatcgagc gcggcatggc ggtgtggctg gaaggtgcgc
     5881 ttaccgatga acagaaacag caatgggcgg ctttgctgca cgaccgcatg accgaatccg
     5941 tattgcccga ttttcagacg gcatccaaat tattccacca tctcgaatcc gaaacctttt
     6001 ccaccgttga tgttttaggc ggcggtaaag aggctttggt caaagccaat accgaaatgg
     6061 gtttggcact ctccgccgac gaaatcgatt atctggttga aaactatcag gctttgcagc
     6121 gcaatccgtc cgatgttgaa ttgatgatgt tcgcgcaggc aaacagcgaa cactgccgcc
     6181 acaaaatctt caacgccgat ttcatcctca acggcgaaaa acaaccgaaa tccctgttcg
     6241 gcatgatacg cgacacgcac aacgcgcatc ccgaaggtac ggtcgtcgct tataaagaca
     6301 attcgtccgt aatcgaaggc gcgaaaatcg agcgtttcta tccggatgcg gcggaaaacc
     6361 aaggctaccg tttccacgag gaagacacgc atatcatcat gaaagtggaa acgcacaacc
     6421 acccgaccgc catcgcgccg tttgcgggcg cggcgacggg cgcgggcggc gaaatccgcg
     6481 acgaaggcgc aacgggcaaa ggttcgcgtc cgaaagcagg cttgaccggc tttaccgtgt
     6541 ccaacctcaa catccccggc ctcaaacagc cgtgggaaca agattacggc aagccggaac
     6601 atatttcctc gccgctggac attatgattg aaggccccat cggcggcgcg gcgttcaaca
     6661 acgaattcgg ccgccccaac ctcttgggct acttccgcac ctttgaagaa aaatttgacg
     6721 gtcaggttcg cggctatcac aaaccgatta tgattgccgg cggtctgggc agcattcagg
     6781 cgcagcagac gcataaagac gaaatccccg aaggcgcgtt gctgatccaa ctgggcggcc
     6841 cgggtatgct tatcggcttg ggcggcggcg cggcttcttc gatggatacc ggtacaaatg
     6901 acgcgtcttt ggacttcaac tccgtgcaac gcggcaaccc cgaaatcgaa cgccgcgcgc
     6961 aggaagtgat cgaccgctgc tggcagctcg gcggcaaaaa cccgattatc tccatccacg
     7021 acgtgggcgc gggcggtttg tccaacgcct tccccgaact cgtcaacgat gccggacgcg
     7081 gcgcggtatt caagctgcgc gaagtgccgt tggaagaaca cggtttaacg cctttgcaaa
     7141 tctggtgtaa cgaatcgcaa gagcgttatg tgttgtcgat tttggaaaaa gatttggaca
     7201 ccttccgcgc catctgcgaa cgcgaacgct gcccgtttgc cgtggtcggc acggcgaccg
     7261 acgacggcca tttgaaagtg cgcgacgatt tgttcgccaa caaccccgtc gatttgccgt
     7321 tgaacgtctt gctcggcaaa ccgcccaaaa ccacgcgtac cgacaaaaca gttacgccat
     7381 ctaaaaaacc gtttcacgcc gacgatatcg acatcaccga agccgcctac cgcgttttgc
     7441 gcctgcctgc cgtagccgcc aaaaacttcc tgattaccat cggcgaccgc agcgtcggcg
     7501 gcatgaccca ccgcgaccaa atggtcggca aataccaaac ccccgtagcc gactgcgccg
     7561 ttaccatgat gggcttcaat acctatcgcg gcgaagcaat gtctatgggc gaaaaaccga
     7621 ccgttgccct gtttgacgcg cccgcctcgg gcagaatgtg cgtcggcgaa gccatcacca
     7681 acatcgcggc agccaatatc ggcgacatcg gcaacatcaa gctctccgcc aactggatgg
     7741 cggcgtgcgg caacgaaggc gaagacgaaa aactctaccg caccgtcgaa gcggtttcca
     7801 aagcctgtca ggcattggat ttgagcatcc ccgtgggcaa agacagcctg tcgatgaaaa
     7861 ctgtgtggca ggacggcgaa gagaaaaaat ccgtggtttc gcccttgagc ctgattatct
     7921 ccgcgtttgc tccagtggaa gacgtacgca agactgttac acccgagttg aaaaacgtcg
     7981 aagacagcgt attgctgttt gtcgatttgg gcttcggcaa agcgcgtatg ggcggctcgg
     8041 cgttcggtca ggtgtacaac aatatgagcg gcgacgcgcc cgatttggac gacacaggcc
     8101 gtctgaaagc gttttacaac gtgattcaac agcttgtcgc cgaagacaaa cttttggcgt
     8161 atcacgaccg cagcgacggc ggtttgtttg cgacactggc ggaaatggcg tttgcggcac
     8221 ggtgcggtat cagtgccgat atagattgcc tgatggataa attcctgccg attcatcacc
     8281 cggatttcca aggcgacccg gccgaggact tatctgacga actttataat catgccgcca
     8341 ttaaaatctt attcaatgaa gaattaggtg cggttatcca aatccgccaa caagataggg
     8401 attatgttga tgcggcattt aaagcggccg acttaaccga tgcggtcagc cggattggct
     8461 ctcctgattt tgacaatgag tttatttctt tctttggtta cggctatttc ttggaacaaa
     8521 accgcgccga cctgcaacgc gcatggcagg aaaccagcca cgccatccaa cgcctgcgcg
     8581 acaaccctgc ctgcgccgac agcgagttcg cattgattgg cgacaacgga cgcagcgcat
     8641 tgtttgccaa cctgaaattc gacgtgaacg aagacatcgc cgcgccgttt atcaacagcg
     8701 gcgcgaaacc caaaatcgcc atcctgcgcg aacagggcgt aaacgggcaa atcgaaatgg
     8761 ccgccgcctt tacccgcgcc ggattcgatg cttacgacgt gcatatgtcc gacctgatgg
     8821 caggccgcat ccacctcgcc gacttcaaaa tgctggcggc gtgcggcggc ttcagctacg
     8881 gcgacgtact cggcgcgggc gaaggctggg cgaaatcgat tctgttccac cctgctctgc
     8941 gcgaccagtt tgccgccttc ttcgccgacc cggacacgct gacattgggc gtgtgcaacg
     9001 gctgccaaat ggtcagcaac cttgccgaaa tcatccccgg cacggcaggc tggccgaagt
     9061 tcaaacgcaa cctgagcgaa cagtttgaag cgcgcctaag catggttcac gtccccaaat
     9121 ccgcgtcgct gattctgaac gaaatgcaag gctccagcct gcccgtcgtc gtcagccacg
     9181 gcgaaggccg cgccgacttc gcacttcacg gcggcaacat ttctgccgat ttgggcattg
     9241 cgctgcaata tgtggacggt caaaaccaag ttacccaaac ctatccgctc aaccccaacg
     9301 gctcgccgca aggcatcgcc ggcattacca acgccgacgg ccgcgtgacc atcatgatgc
     9361 cccaccccga acgcgtgtac cgcgccgcac aaatgagctg gaaaccggaa ggctggacgg
     9421 aactgtccgg ctggtaccgc ctctttgccg gcgcacgtaa agccttgggc taaccgccct
     9481 actcaaacca atgccgcctg aagaatattt cagacggcgt tccggcatac catcctttaa
     9541 acggtatccg tccaccgagg aacactcatg aaaatcaccc ccgtcaaagc cctaaccgac
     9601 aactacatct ggatgataca gcacggcaac catgccgtct gcgtcgaccc ttccgaaccc
     9661 tcgcccgtct tggaattcct cgtccgcaac cgcctcatgc ttgcccaaac atgggtaacg
     9721 cacccccatc ccgaccacga gggcggtgcg gcggcactct ggcgcggcta catggaatcg
     9781 cccgtttacg gcgaatccga catcgaagca gcaacccaca ccgtaaccgc cggcacccaa
     9841 ttcaccttcg gcgaagggtt aatcaccgtt tgggcaaccc cgggccacac agaccgccac
     9901 accagctacc ttctcgaaac ttcagacggc atacacgtct tttgcggcga cacccttttt
     9961 tccgccggct gcggacgcgt gtttaccggc acaatcgaac agctttacga cagcttccaa
    10021 cgcttcaacc aattacccga aggcaccctg ttctacccgg cgcacgaata caccgccgcc
    10081 aacctgcgtt tcgccgccca tatcgagccg gacaacgccg acattcagac ggcactgaaa
    10141 gcggcagaac acacgcctac cctgcccgtt accctcgcgc acgaacgccg cgtcaacccg
    10201 tttttgcgcg tcgacctgcc gcacgtcaga gaccgcgccg aggcattgag cgggaaaacg
    10261 ttaaacagca gcctcgatac ctttgtcgcg ctgcgtgaac ttaaaaacca ataccggacg
    10321 aaataaaaca acgggaaaac gcagccattc ctaggatttt tattaaaatc ttaaataaaa
    10381 tcatacaatc atcgccaata gacgaaagga caccgttgcc ttataatcaa acaaaaacaa
    10441 aatatataat atagtggatt gaatt
//
LOCUS       DAXHSF010000057        10443 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.057, whole genome shotgun sequence.
ACCESSION   DAXHSF010000057 DAXHSF010000000
VERSION     DAXHSF010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10443
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.057"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(5..1006)
                     /locus_tag="ACFM8S_001058"
     CDS             complement(5..1006)
                     /locus_tag="ACFM8S_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFC6314140.1"
                     /translation="MIDGQTTEPKQKTRIILAPMQGLVDDVMRDLLTRIGGYDECVSE
                     FVRITHTVHSQSIWLKYVPEIANGNKTFSGTPCTVQLLGSDADNMAANALEAVRFGAN
                     KIDLNFGCPAPTVNKHKGGAILLKEPELIFHIVKTLRGRLPAHIPLTAKMRLGYEDKS
                     RALECACAIAEGGACGLTVHARTKAEAYEPPAHWEWIRKIRDTVNIPVTANGDVFSLQ
                     DYIGIKTISGCNSVMLGRGAVIRPDLARQIKQYENGGPVKDTDFAEVSKWIRQFFELC
                     LTKEANNKYPLARLKQWLGMMKKEFAAAQNLFDRVRTVKDADEVRNILAEFEREMNT"
     gene            complement(1083..4916)
                     /locus_tag="ACFM8S_001059"
     CDS             complement(1083..4916)
                     /locus_tag="ACFM8S_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HFC6314141.1"
                     /translation="MTTTTAPQRIREIPYNYTSYTDREIVIRLLGDEAWQILQDLRGQ
                     RKTGRSARMLFEVLGDIWVVVRNPYLVDDLLEHPKRRAALVREMRHRLNEIRKRRDDN
                     RQVDVLVAAAEKAVERFDSSFDETSQKRRQILERLSKITKPHNIMFDGLARVTHVTDA
                     TDWRVEYPFVVVNPDTEAEIAPLVRALIELDLVIIPRGGGTGYTGGAIPLDAMSAVIN
                     TEKLDKHRGVEYVELAGLDGKHPIIRCGAGVVTRRVEETAHQAGLVFAVDPTSADASC
                     VGGNVAMNAGGKKAVLWGTALDNLAYWNMVNPQGEWLRIERVRHNFGKIHDEETAVFD
                     VHTLDSDGINIVKTERLEIPGHKFRKVGLGKDVTDKFLSGLPGVQKEGTDGIITSVAF
                     VLHKMPKYTRTVCMEFFGTVATATPSIVEIRDFLLAHESVRLAGLEHLDWRYVRAVGY
                     ATKAAGKGRPKMVLLADVVSDDEAAVQAAAEHICELARARDGEGFIAVSPEARKTFWL
                     DRSRTAAIAKHTNAFKINEDVVIPLERLGEYSDGIERINIELSIQNKLKLCAALEQYL
                     SGKLPIDKMGTDLPTAELLGERGKHALAHVSAVKARWDWLLANLDTPLADYKARYGAA
                     VHAAPEAKDDESCFIAFRDFRLRVSVKADVMKPLAEIFSGKTDTKIIQGLGKIHAKTV
                     RSRVFVALHMHAGDGNVHTNIPVNSDDADMLQTAYRSVERIMKIARSLGGVISGEHGI
                     GITKLEFLTDEDLQPFWNYKNQVDPKHTFNRHKLMKGSDLRNAYTPSFELLGAESLIM
                     EKSNLGTIADSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILGVGLLTEAFLYEEQT
                     RRGVSIKHFEELMDIGDHCTVCHRCVKPCPVNIDFGDVTVAVRNYLADSGHKRFAPAA
                     SMGMAFLNATGPKTIKALRAAMIQTGFPAQNFAYKIGKLLPVGTKKQKAEPKATVGKA
                     PIKEQIIHFINRPLPKNVPAKTPRSLLGIEDGKSIPIIRNPASPEDAEAVFYFPGCGS
                     ERLFSQIGLAVQAMLWHVGVQTVLPPGYMCCGYPQDAGGNKAKAEEMSTNNRVAFHRM
                     ANTLNYLDIKTVVVSCGTCYDQLEKYRFEEIFPGCRIIDIHEYLLEKGVKLDGVKGQQ
                     YLYHDPCHTPIKTMNATQMASNLMGQKVVLSDRCCGESGMFAVKRPDIATQVKFRKQE
                     EIEKNLKELPQGEPVKMLTSCPACLQGLSRYTDDNNMPADYIVIEMAKHILGENWLDE
                     FVKKANNGGVEKVLL"
     gene            5177..5273
                     /gene="ffs"
                     /locus_tag="ACFM8S_001060"
     ncRNA           5177..5273
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACFM8S_001060"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            5441..7132
                     /gene="dld"
                     /locus_tag="ACFM8S_001061"
     CDS             5441..7132
                     /gene="dld"
                     /locus_tag="ACFM8S_001061"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFC6314142.1"
                     /translation="MSASQLLSRLTQTVGEKYIITDPAKTEQYRQGYRFGEGKALAVV
                     RPGSILEMWKILQACVEADVIVITQAANTGLTGGSTPDGNDYDRDIVIVNTMRMNIIQ
                     TINDNEQVVCLPGSTLNQLELLLKPLGREPHSVIGSSCIGASVLGGVCNNSGGALVQR
                     GPAYTEMALFAQINEEGRLELVNHLGIDLGDTPEEILTNLQGHHYQKKDIKQDAGKGH
                     DHAYCEHVRQVDEPTAARFNADPARHYEASGCAGKLMVFAVRLDTFPQEKQTAVFYIG
                     TNDINELTDIRRAALGEFESLPVSGEYIHRHAFDIADVYGKDTFYVIKKFGTHQLPKL
                     FDLKARVDRFGKKASFLPKHFSDKAMQFVSKYLPDHLPKSMRDYRDKYEHHLILKMGG
                     KGVDEARAFLKEYFAHHSGAFFECNAEETQAAMLHRFAVASAAIRYRAVHDDEVEDLV
                     ALDIALRRDDRDWFEKLPPEIDNKIIHKLYYGHFMCHVFHQDYIIKKGNDCMALEHEM
                     LHLLDQRGAQYPAEHNVGHLYEAKPALKQFYRKLDPTNSFNPGIGKTSKKKNWAE"
     gene            7264..7371
                     /locus_tag="ACFM8S_001062"
                     /pseudo
     CDS             7264..7371
                     /locus_tag="ACFM8S_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            7427..7798
                     /locus_tag="ACFM8S_001063"
     CDS             7427..7798
                     /locus_tag="ACFM8S_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HFC6314143.1"
                     /translation="MKPYKIYTHPALLPQAVKQGWSWPGLLFGTLWACFKRMWGLGLG
                     LTGAIFVLAVFAQLVYGDTPATDSAFNVLGLAVSVWFGAKGNSLYARHLLSRGYTELP
                     ETVEAANPQAALAQYFGRGGR"
     gene            7953..8501
                     /locus_tag="ACFM8S_001064"
     CDS             7953..8501
                     /locus_tag="ACFM8S_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFC6314144.1"
                     /translation="MARLTVHTLETAPEAAKARVEAVLQNNGFIPNLIGVLSNAPEAL
                     AFYQEVGKLNAANSLTAGEVEVIQIIAARTNQCGFCVAGHTKLATLKKLLSEQSVKAA
                     RALAAGEFDDAKLGALAAFTQAVMAKKGAVSDEELKAFFDAGYNQQQAVEVVMGVALA
                     TLCNYVNNLGQTEINPELQAYA"
     gene            8636..9727
                     /locus_tag="ACFM8S_001065"
     CDS             8636..9727
                     /locus_tag="ACFM8S_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HFC6314145.1"
                     /translation="MNAQTLIANVAEFVKTKLKPIVDDIDRKGYYPEAFMRELGAIGG
                     FGAVGTEAEGGNGLGLATQIAVLREIGKECGATSFSAWCQAACAWYLHQTPNRAVKDK
                     YLADILQGKVLAGTGMSNTVKHLADIEKHNLQAERVEGGYTVNGALPWVSNIGEDHIW
                     ANTAQIGGGYVMFITGGQWEGVSLQNCPEFCALEGTRTFSLNFKDVFIPDEDIIAAPE
                     QFADYIQSIKAGFILLQIGIGAGVIDGSLGIIRLANVVNAEVNRYLDDGYDSLKTRLD
                     GAWAETERLAGLAWSGTPDNLTTLKLREAAAVLALAAAQSAALHSGAKGYLMRSPAQR
                     RVREAMFVAIVTPAIKHLRKEIAAIEAAK"
     gene            10189..10359
                     /locus_tag="ACFM8S_001066"
     CDS             10189..10359
                     /locus_tag="ACFM8S_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HFC6314146.1"
                     /translation="MAQYMCGPCGWIYDEEHGDPEHGIPPGTKFEDIPDDWKCPECGV
                     GKEDFYLLDFVI"
BASE COUNT         2428 a         2907 c         2820 g         2288 t
ORIGIN      
        1 atattcaagt attcatttct cgctcaaatt cagccaagat gttccgaact tcgtccgcat
       61 ccttaaccgt tcggacgcgg tcgaacagat tttgtgctgc tgcaaattct ttcttcatca
      121 tacccagcca ctgtttcagc cgcgccagcg gatatttgtt gtttgcctct tttgtcaggc
      181 acagctcgaa aaactgccgt atccatttgg aaacttcggc aaaatccgtg tctttgaccg
      241 gcccgccgtt ctcgtattgc ttgatttgcc gcgccaaatc ggggcggatg accgcgccgc
      301 gaccgagcat cacgctgttg cagccgctga ttgttttgat gccgatatag tcctgcaggc
      361 tgaaaacgtc gccgttggcg gtaacgggaa tattgacggt atctcggatt ttccttatcc
      421 attcccaatg cgccggcggt tcgtaagcct cggctttggt acgcgcgtgt acggtcagtc
      481 cgcatgcgcc cccttcggca atcgcacagg cgcattccaa agcccggctt ttatcttcat
      541 agccgagccg catttttgcg gtgagcggaa tatgtgcggg caaacgtccg cgcagcgttt
      601 tgacgatgtg gaatatcagt tccggctctt ttaaaaggat tgcgccgcct ttgtgtttgt
      661 tgacggtggg cgcggggcag ccgaagttca aatcgatttt gttcgcaccg aagcggacgg
      721 cttccagcgc attcgccgcc atattgtccg catcgctgcc caaaagttgg acggtgcagg
      781 gcgtgccgga aaacgttttg tttccgttgg cgatttcggg gacatatttt aaccatatgg
      841 attgggaatg cacggtatgg gtaatgcgta caaattcgct gacgcattcg tcgtagccgc
      901 caatacgcgt cagcaggtcg cgcatcacgt catcgaccag accctgcata ggggcaagga
      961 tgattcgggt tttttgtttg ggttcggttg tttgtccgtc aatcatgatt cgggtaggta
     1021 tttgattcaa acaatgccgt ctgaaggctt tcggcgttca gacggcattt ccgtgtccgt
     1081 tgttacagca atactttttc cacgccgccg ttgttggctt ttttcacaaa ctcgtctagc
     1141 cagttttcgc cgaggatgtg tttcgccatt tcgatgacga tgtagtcggc aggcatattg
     1201 ttgtcgtcgg tgtagcggct caagccttgc aggcaggcgg ggcaggaggt cagcattttg
     1261 acgggttcgc cctgcggcag ctctttgagg tttttctcga tttcttcctg tttgcggaat
     1321 ttgacttggg tggcgatgtc ggggcgtttg acggcaaaca taccggattc gccgcagcag
     1381 cggtcgctta aaacgacttt ctgccccatc aggttgcttg ccatttgggt ggcgttcatg
     1441 gttttgatgg gtgtgtggca ggggtcgtgg tagaggtatt gctgaccttt cacgccgtcg
     1501 agtttcacgc ctttttcaag caggtattcg tggatgtcga tgatgcggca gccggggaag
     1561 atttcctcaa atcggtattt ttcgagctgg tcgtaacaag tgccgcaact gacgacgacg
     1621 gttttgatgt cgaggtagtt gagggtgttt gccatacggt ggaaagccac gcggttgttg
     1681 gtgctcattt cttcggcttt tgccttattg ccgcccgcgt cctgcggata gccgcaacac
     1741 atatagccgg gcggcaggac ggtttgcacg ccgacgtgcc agagcatggc ttggacggcg
     1801 agtccgattt ggctgaacag gcgttccgaa ccacagccgg gaaagtaaaa cacggcttcg
     1861 gcatcttccg gagaagccgg attgcggatg atggggatgc ttttgccgtc ttcgatgccc
     1921 aataaggagc gcggtgtttt ggcaggcacg tttttgggca gcggacggtt gatgaagtgg
     1981 ataatttgtt ctttaatcgg ggctttgccg acggttgcct tgggttcggc tttttgcttt
     2041 ttcgtgccga cgggcagcag tttgccgatt ttgtaggcga aattctgcgc ggggaagccg
     2101 gtttgtatca tcgcggcacg tagggcttta atggttttcg ggccggtcgc gtttaggaat
     2161 gccataccca ttgaagctgc cggcgcaaag cgtttgtggc cggaatcggc aaggtagttg
     2221 cggacggcta cggtaacgtc gccgaagtcg atgttgacgg ggcaaggctt gacgcagcgg
     2281 tggcacacgg tgcagtggtc gccgatgtcc atgagttctt caaagtgttt gatggaaacg
     2341 ccgcgacggg tttgttcttc gtataaaaag gcttcggtca agagccccac gccgaggatt
     2401 ttgttgcgcg gactgtacag caggttggcg cgcggaacgt gggtggagca gacgggtttg
     2461 catttgccgc agcgcaggca gtctttgacg gaatcggcaa tcgtgccgag gtttgatttt
     2521 tccataatca gcgattccgc gcctaacagc tcgaaagacg gtgtgtaggc gttgcgtaag
     2581 tccgagcctt tcatcagttt gtggcggttg aaggtgtgtt tggggtcgac ttggtttttg
     2641 tagttccaaa acggttgcaa atcttcatcg gtcaaaaatt cgagcttggt aatgccgatg
     2701 ccgtgttcgc cggaaatcac gccgccgagc gaacgggcga ttttcataat gcgttcgacc
     2761 gaacggtagg ctgtctgaag catgtcggca tcgtctgaat tgaccggaat attggtgtga
     2821 acgttaccgt cgccggcgtg catatgcagg gcgacgaaga cgcggctgcg tacggttttg
     2881 gcgtggattt tgcccaagcc ttggataatt ttggtatcgg ttttgccgct gaagatttcg
     2941 gcaagcggct tcattacgtc tgctttgaca gaaacgcgca ggcggaaatc gcggaaagca
     3001 ataaagcaac tttcatcgtc tttggcttcg ggcgcggcgt ggacggctgc gccgtagcgg
     3061 gccttgtagt cggcaagcgg cgtgtcgaga ttggcgagca accagtccca acgcgctttg
     3121 acggcggaaa cgtgggccag ggcgtgtttg ccgcgttcgc ccaaaagttc ggcggtcggc
     3181 aggtcggttc ccattttgtc gatggggagt ttgcccgaaa gatattgctc caaggcggca
     3241 cagagtttga gtttgttttg aatggaaagc tcgatgttga tgcgttcgat accgtctgaa
     3301 tactcgccga gcctttcgag tgggatgacc acgtcttcgt tgattttaaa ggcgttggta
     3361 tgtttggcga tggcggcggt gcggctgcgg tcgagccaga aggttttgcg cgcttcgggc
     3421 gatacggcga taaagccttc gccgtcgcgg gcgcgggcaa gttcacagat gtgctcggcg
     3481 gctgcctgta cggcggcttc gtcgtcggaa accacgtccg ccagcaagac cattttcggc
     3541 cgccccttgc ctgctgcttt ggtggcataa ccgacggcac ggacgtaacg ccagtccaaa
     3601 tgttccaaac ccgccagccg tacgctttca tgggcaagca aaaagtcgcg gatttcgaca
     3661 atagatggcg tggcggtggc aaccgtgccg aaaaactcca tacacacggt gcgcgtgtat
     3721 ttcggcattt tgtgcaatac gaaggcaacg ctggtgatga tgccgtctgt gccttctttc
     3781 tgcacgccgg gcagtccgct caagaatttg tcggtaacgt ctttgcccaa accgactttg
     3841 cggaatttgt ggccggggat ttccaagcgt tcggttttaa cgatattgat gccgtctgaa
     3901 tccagcgtgt gaacgtcgaa cacggcggtt tcttcgtcgt ggattttgcc gaaattgtgg
     3961 cgcacgcgct cgatacgcag ccattcgcct tgagggttaa ccatgttcca gtaggcgagg
     4021 ttgtccaacg ccgtccccca cagcacggct tttttgccgc ccgcgttcat cgccacatta
     4081 ccgcccacgc atgacgcgtc ggcagaagtc ggatcgacgg cgaacaccaa acctgcctga
     4141 tgcgcggttt cttccacccg ccgcgtaacc acgcccgcgc cgcaccggat aatcggatgc
     4201 ttgccgtcca agcctgccag ctcaacgtat tcaacaccac gatgcttgtc gagtttttcg
     4261 gtattgatga ctgcgctcat tgcgtccaaa ggaatcgcgc cgccggtata acccgtgccg
     4321 ccgccgcgcg gaataatgac caaatccagc tcgattaagg cgcgcaccaa aggcgcgatt
     4381 tccgcctccg tgtcgggatt gacgacgaca aacggatact ccacgcgcca gtcggttgca
     4441 tcggtaacgt gcgttacccg cgccagcccg tcgaacataa tattgtgcgg cttggtgatt
     4501 ttgctcaaac gctccaaaat ctgccgccgt ttttggctgg tttcatcaaa actgctatca
     4561 aaacgctcga ctgctttttc tgctgcggca accaacacat ccacttgccg attatcgtcg
     4621 cggcgtttgc ggatttcatt taagcggtgg cgcatttcac gtaccagcgc ggcgcggcgt
     4681 tttgggtgct ccagcaagtc atcgaccaga tacggattgc gcacgaccac ccaaatatca
     4741 cccaacactt caaacagcat ccgcgccgaa cgcccggttt tgcgttgacc gcgcaggtct
     4801 tgcaggattt gccacgcttc gtcgcccaat aatcggatga cgatttcgcg gtcggtgtag
     4861 gaagtatagt tgtaggggat ttcccgaata cgctgagggg cggtagtcgt ggtcatggtg
     4921 tgtccgtgtt tggcagtttt tatttgaatg ccgtctgaaa agtcggtttc aggcgagttt
     4981 gaaaaggcga taatgtagtt caatttttga tacttttcaa tgccgaaaca ggaaataccc
     5041 ttatagaaac aaaataacgc tttgagaaat tgttgacaat attatcaaat ataaatttta
     5101 ggtggtgttt ccggcgtatg accgtatcaa aacggttgag tttttcagaa aacccgttat
     5161 aatgccgttt ctctgaggcg ggtctccccg catggcaaat cggaacaccg ggtcaggggc
     5221 ggaagccagc agcccactcc gatgcgccag tgccgggggt ttggcccgcc gccctatttg
     5281 aaacgccgga gcttcgatgt tccggcgttc ccgtatcgca ccgatgccgt ctgaaagccg
     5341 ttcagacggc attttcttta ctttgccata acgtataact acaatgtgaa aacaattcca
     5401 atgtgcatat gcacctgccg acaattttaa ggaaactgcc atgagcgcat cacaattact
     5461 gtcaagactg acccaaaccg taggcgaaaa atacatcatc accgaccccg cgaaaaccga
     5521 acaataccgc cagggctacc gcttcggcga gggtaaggcg ttggcggtgg tgcgccccgg
     5581 aagcattctg gaaatgtgga aaattctaca ggcgtgcgtc gaagcggacg tgattgtgat
     5641 tacgcaggcg gcgaataccg gtttgacggg cggctcgacc cccgacggca acgattacga
     5701 ccgcgacatc gtgatagtga acactatgcg gatgaacatc atccaaacca tcaacgacaa
     5761 cgaacaagtc gtctgcctgc ccggttcgac cctgaaccag cttgaattgc tgctgaaacc
     5821 tttggggcgc gaaccgcatt cggtcatcgg ctcttcctgc atcggcgcgt ccgtcttggg
     5881 cggcgtgtgc aacaactccg gcggcgcgtt ggtacagcgc ggtccggcct acaccgaaat
     5941 ggcgttgttc gcccaaatca acgaagaagg caggttggaa ctggtcaacc acttgggcat
     6001 agacttgggc gacacgcccg aagaaatcct gaccaacctg caaggtcatc attatcagaa
     6061 aaaagacatc aaacaagacg cgggcaaagg acacgaccac gcctattgcg aacacgtccg
     6121 ccaagtggac gaaccgaccg ccgcgcgttt caatgccgac cccgcccgcc attacgaagc
     6181 ctcgggctgc gcgggcaaac tgatggtttt tgccgtccgt ttggacacct tcccgcaaga
     6241 aaaacaaacc gccgtgttct acatcggcac gaacgacatc aacgagctga ccgacatccg
     6301 ccgcgccgcc ttgggcgaat ttgaaagcct gcctgtttcc ggcgaataca tccaccgcca
     6361 cgctttcgat attgccgacg tgtacggcaa agacacgttt tacgtcatca aaaaattcgg
     6421 tacgcaccaa ctgccgaaat tatttgactt gaaggcgcgc gtggacaggt tcggcaaaaa
     6481 agccagcttc ctgcccaaac atttttccga caaggccatg cagttcgtca gcaaatacct
     6541 gcccgaccac ctgcccaaat ccatgcgcga ttaccgcgac aaatacgaac accacctgat
     6601 tctgaaaatg ggcgggaaag gcgtggatga ggcgcgcgcg ttcttaaaag aatattttgc
     6661 acaccacagc ggcgcgtttt tcgagtgcaa cgccgaagaa acccaagccg cgatgctgca
     6721 ccgtttcgcc gtcgcctccg ccgccatccg ctaccgcgcc gtgcatgatg acgaagtgga
     6781 agacttggtc gcgcttgaca tcgccctgcg ccgcgacgac cgcgactggt ttgaaaaact
     6841 gccgcccgaa atcgacaata aaatcatcca caaactctac tacggacatt tcatgtgcca
     6901 cgttttccat caggattaca tcatcaaaaa aggcaacgac tgcatggcgt tggaacacga
     6961 aatgctgcat ctcttagacc aacgcggcgc gcaatatccc gccgaacaca acgtcggtca
     7021 tttgtatgaa gccaagcccg cgctcaaaca gttttaccgc aaactcgacc cgaccaacag
     7081 cttcaacccg ggtatcggca aaaccagcaa aaagaaaaac tgggcggaat aagcgcgtcc
     7141 gctttgaagg caggcaatgc cgtctgaagg caaaacgccg ttcagacggc attgtcgggt
     7201 ttgaagttcc ccgtcgggta atcgctttca aaccgtatgc cgacacacgg gcgcgcccct
     7261 cccaaatata gtggattaaa tttaaatcag gacaaggcaa cgaagccgca gacagtacag
     7321 atagtacggc aaggcgaggt aacgccgtac tggtttaaat ttaatccact ataaagtctg
     7381 atcctacctt cccaaagggc agggtttcaa ccgaaaagga aacacgatga aaccatacaa
     7441 aatctacacc catcccgccc tgctgcccca agccgtcaaa caaggctggt catggccggg
     7501 gctcttgttc ggcacgctgt gggcgtgttt caaaagaatg tggggtttgg ggctggggct
     7561 gacgggcgcg atattcgtgc tggctgtgtt tgcccaactg gtttacggcg acacgcccgc
     7621 cacagactcg gcgtttaacg tattgggctt ggcggtttcc gtctggttcg gcgcaaaggg
     7681 caacagcctt tatgcacgcc accttttatc gcgcggctat accgaattgc ccgaaacggt
     7741 tgaggcagcc aatccccaag ccgcattggc gcaatatttc gggcgcgggg gccgataagc
     7801 gcgttttctt ttccatcatg ccgtctgaaa aatgttcaga cggcatttct tataaccttt
     7861 tgtttgcagc acataaccaa tcagtctttc ctattccgtt ccgaaatgca taccatgcgc
     7921 ccgtacccaa tatttgcgaa caaggaaaga aaatggcacg tttaaccgta cacaccctcg
     7981 aaaccgcccc cgaagccgcc aaagcgcgcg tcgaggcggt acttcaaaac aacggcttta
     8041 tccccaacct tatcggcgta ttatcaaacg cccccgaagc cttggcgttt taccaagaag
     8101 tcggcaagct caacgccgcc aacagcctga ccgccggcga agtcgaagtc atccagatca
     8161 tcgccgcccg taccaaccaa tgcggttttt gcgtggcagg gcacaccaaa ctcgcaaccc
     8221 tgaaaaaact cctgtccgaa caatccgtca aagccgcgcg cgctttggcg gcaggcgaat
     8281 ttgacgatgc taaactcggc gcgctcgccg cctttaccca agccgtaatg gcaaaaaaag
     8341 gcgcggtatc cgacgaggaa ctcaaagcat tttttgatgc gggctacaac cagcagcagg
     8401 cagtcgaagt cgtgatgggc gtagccttgg caactttgtg caactacgtc aacaacctcg
     8461 gacaaaccga aatcaacccc gaattgcagg cttacgcctg atacggcaaa ccgcccgaat
     8521 atcgggcggt ttttcaaaaa caccccctca aacaaaaaca agccgcctaa gcggcaggga
     8581 agcagccgcg catcaggcgc gcccccgatt cccccgcaaa ccttaaggaa caaagatgaa
     8641 cgcccaaacc ctgattgcca acgttgccga attcgtcaaa accaagctca aacccatagt
     8701 ggacgacatc gaccgcaaag gatactaccc cgaagcattt atgcgcgaac tcggcgcaat
     8761 cggtggattc ggcgcagtcg gtacagaagc cgaaggcggc aacggcttgg gtttggcaac
     8821 gcaaatcgcc gtattgcgcg aaatcggcaa agaatgcggc gcaacctctt tcagtgcgtg
     8881 gtgtcaggcg gcttgcgcgt ggtatctgca ccaaacgccc aaccgggccg tcaaagacaa
     8941 ataccttgcc gacatcctgc aaggcaaagt attggcgggc acaggaatgt ccaataccgt
     9001 caaacacctt gccgacatcg aaaaacacaa cctccaagcc gagcgcgtgg agggcggcta
     9061 cacagtcaac ggcgcgctgc cgtgggtgtc caacatcggc gaagaccaca tttgggcgaa
     9121 taccgcccaa atcggcggcg gctacgtcat gttcatcaca ggcggacaat gggaaggcgt
     9181 aagcctgcaa aactgccccg aattttgcgc cctcgaaggc acgcgcacct tcagcctgaa
     9241 cttcaaagac gtatttatcc ccgacgaaga catcatcgcc gcgcccgaac agtttgccga
     9301 ctacatccaa agcatcaaag caggctttat cctcctgcaa atcggcatcg gcgcgggcgt
     9361 gatagacggc agcctcggca tcatccgcct cgccaacgtc gtcaacgccg aagtcaaccg
     9421 ctatctcgac gacggctacg acagcctcaa aacaaggctg gacggcgcgt gggcagaaac
     9481 cgaacggctc gccggattgg cttggagcgg cacgcccgac aaccttacca ccctcaagct
     9541 gcgcgaagcc gccgccgtac tggcacttgc cgccgcccaa tccgccgccc tgcattccgg
     9601 cgcaaaaggc tacctgatgc gcagtcccgc ccaaaggcgc gtccgcgaag caatgtttgt
     9661 cgccatcgta acccccgcga tcaaacacct ccgaaaagaa atagcggcaa tcgaagccgc
     9721 aaaataaaca ggcggaaaaa accgaaaccc ccaccgcgtc attcccgcga aagcgggaat
     9781 ccagccccca acgcaacagg aatccatcgg aaaaaacaga aacccccaaa ccgtcattcc
     9841 cgcaaaagcg ggaatccagc ccccaacgca acaggaatcc atcggaaaaa acagaaaccc
     9901 tcaaaccgtc attcccgcaa aagcgggaat ccagcccccc ctaacgcaac aggaattcat
     9961 cgaaaaaacc gaaacctcca aaccgtcatt cccacggaag tgggaatcca gtaatcgaaa
    10021 aaccacagga atctatcgaa aaaaacccca acccccaaaa aaaccgggcg gataccgcac
    10081 catcctccca aaccctgatt tatagtggat gaacaaaaat caggacaagg cgaggcaacg
    10141 ccgtaccggt ttttgttcat ccgctataca aaaaacaagg aaacacaaat ggcgcaatat
    10201 atgtgcggcc cctgcggctg gatttacgat gaagaacacg gcgaccccga acacggcatc
    10261 ccccctggga caaagtttga agacatcccc gacgactgga aatgccccga atgcggcgta
    10321 ggtaaagaag atttctacct gttggatttc gtgatatagc ggcagccgca cccttaggga
    10381 aaaccccgtc atccggcgtt ccgcctgcgg cggcagggaa cgccgtttta tagtggatta
    10441 aca
//
LOCUS       DAXHSF010000058        10409 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.058, whole genome shotgun sequence.
ACCESSION   DAXHSF010000058 DAXHSF010000000
VERSION     DAXHSF010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10409
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.058"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(19..222)
                     /locus_tag="ACFM8S_001067"
     CDS             complement(19..222)
                     /locus_tag="ACFM8S_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314147.1"
                     /translation="MDTTLKHKAEALLGEPLLDEPVRPESWECCGSDCGDACIQTIYW
                     ADKARYDAQRRKLKEAGWSDDAV"
     gene            complement(200..433)
                     /locus_tag="ACFM8S_001068"
     CDS             complement(200..433)
                     /locus_tag="ACFM8S_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFC6314148.1"
                     /translation="MEATVYLEDNEYIALCDLLKLAGLAESGGQAKALIAEGLVSRNG
                     ETETRKTAKIRGGEVIEFDGARLEIADGYDPEA"
     gene            966..4400
                     /gene="dnaE"
                     /locus_tag="ACFM8S_001069"
     CDS             966..4400
                     /gene="dnaE"
                     /locus_tag="ACFM8S_001069"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFC6314149.1"
                     /translation="MTEPTYIPLRLHTEFSITDGMVRIKKLIAKAQEYGLPALGISDL
                     MNEFGLVKFYKACRSAGIKPIGAADVWIGNPNAPDKPFRAMLIIRNDAGYLRLSELLT
                     EAYVGKDRNIHHAELNPEWLENGDNSGLICLSGAHYGEVGVNLLNGNEDAARAAALKY
                     AAWFPDAFYMELQRLPERPEWEACVSGSVKLAEELGLPVVATHPTQFMSRDDFNAHEA
                     RVCIAGGWVLTDKKRPRDFTPDQFFIPPETMLERFADLPEALENTVEIAKRCNLHITL
                     GKNFLPLFPTPDGLSRDDYLIKLSNEGLQERMVQLYPDEAERAAKMPEYQERLDFELN
                     IIIQMKFPGYFLIVQDFINWAKTHGCPVGPGRGSGAGSLVAYSLKITDLDPLKYALLF
                     ERFLNPERVSMPDFDVDFCQANRGRVIEYVRDKYGAQAVSQIVTFGTMSSKAVIRDVG
                     RVLELPFMLCDKLSKLIPLEANKPLSLDDAMKAEPQIQELIEAEEADELITLAKKLED
                     LTRGLGMHAGGVLIAPGKISDYSPVYQADESASPVSMYDKGDVEDVGLVKFDFLGLRN
                     LTIIEMAQNNIKNTTGDIIDVGKIPLDDQAAYQIFRDANTTAVFQFESTGMKKMLKTA
                     HTTKFEELIAFVSLYRPGPMDNIPDFVARMKGQEFQYIHPLLEGILAPTYGIMVYQEQ
                     VMQAAQIIGGYSLGGADLLRRAMGKKKPEEMVKHREIFAEGAAKQGISREKSDEIFNY
                     MEKFAGYGFNKSHAAAYALISYQTAWLKAHYPAEFMAATMSSELDNTDQLKHFYDDCR
                     ANGIEFLPPDINESDYRFTPYPNMKIRYALGAIKGTGEAAVESIIAARQSGGKFTGLL
                     DFCERVGKEHMNRRTLEALIRGGAFDSIEPNRAMLLANIDLAMDNADQKAANANQGGL
                     FDMMEDAIEPVRLVDAPMWSESEKLAEEKTVIGFYLSGHPFGPYAQEVRQIAPTKLDR
                     LKPQDSVRLAGFVTAVRTMMGKRGKIAFVSLEDLSGQVEIMVGGQTLENCADCLKADQ
                     VLIIESKVSRDDYGGGDGLRILANQVMTLQTARERYARSLSLALAPHHDIGGLVQLLA
                     AHQLPDTPRIPLQLSYANGKASGRFQVPPKWTVTPSSALFDELEKLLGSRSVRVNW"
     gene            4678..7927
                     /gene="cas9"
                     /locus_tag="ACFM8S_001070"
                     /gene_synonym="csn1"
                     /pseudo
     CDS             4678..7927
                     /gene="cas9"
                     /locus_tag="ACFM8S_001070"
                     /gene_synonym="csn1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742773.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes.;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
     gene            7994..8908
                     /gene="cas1"
                     /locus_tag="ACFM8S_001071"
     CDS             7994..8908
                     /gene="cas1"
                     /locus_tag="ACFM8S_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HFC6314150.1"
                     /translation="MTWRSLLIQNGGKLSLQRRQLLIQQNGESHTVPLEDIAVIIIEN
                     RETLITAPLLSALAEHGATLLTCDEQFLPCGQWLPYAQYHRQLKILKLQLNISEPLKK
                     QLWQHIVRQKILNQAFVADETGNDLAAKRLRTLASEVRSGDTGNREAQAAALYFQALF
                     GEKFTRNDNNAVNAALNYTYAVLRAAVARALTLYGWLPALGLFHRSELNPFNLADDFI
                     EPLRPLADLTVIHLYEQGRLKTELTLGIKQHLIKILYYQTSIERQHFSTLAAIDKMIS
                     SFQAGVTDKNAKQLKLPEILPLKEYQYE"
     gene            8901..9227
                     /gene="cas2"
                     /locus_tag="ACFM8S_001072"
     CDS             8901..9227
                     /gene="cas2"
                     /locus_tag="ACFM8S_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HFC6314151.1"
                     /translation="MSEAKFMRIIVFFDLPVITAAKRKAANQFRQFLLKDGYQMLQLS
                     VYSRIVKGRDSLQKHHNRLCANLPQEGSIRCLEITEKQYAAMKLLLGELKTQEKKVNS
                     DQLLLF"
     repeat_region   9291..10382
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=9291..9326
                     /rpt_unit_seq="attgtagcactgcgaaatgagaatgggagctacaac"
BASE COUNT         2913 a         2769 c         2521 g         2206 t
ORIGIN      
        1 tatcaagccg aaccgttttc agacggcatc gtccgaccaa cccgcttctt tcaatttccg
       61 ccgttgcgcg tcgtagcggg ccttgtccgc ccagtaaatc gtctgaatgc acgcgtcgcc
      121 gcagtcgctg ccgcagcatt cccacgactc ggggcggacg ggttcgtcta aaagcggctc
      181 gcccaaaagg gcttcggctt tatgcttcag ggtcgtatcc atcggcgatt tccaagcgcg
      241 cgccgtcaaa ctcgatgact tcgccgccgc gtattttggc ggttttacgg gtttcggttt
      301 cgccgttgcg cgacaccaac ccttcggcaa tcaacgcctt cgcctgtccg ccgctttcgg
      361 caagtccggc caatttcaag aggtcgcaca gggcgatgta ttcgttgtct tcgagataga
      421 cggtggcttc cataatgttc ccttgcagaa agaggccgtt attgtagcac ctgccgccgc
      481 cgtacccaaa attaccgcaa aacaggcagc acatcccgcc ccgcctgttc cgtaaaaatg
      541 ccgtctgaaa ccccatatgc cgcaatccga tcaaagaaat cctgtccaac ggcagactgc
      601 aaatcctgtt ccccgacgaa tccacattga cgctgatgca catcctcaaa cgcgaactgc
      661 ccgatacacc ggcaatcggc atcaaaacga aatcaaaaac ctgtttgaac gtaaaattaa
      721 tttaactgct tgccatccgt ccgaataagg tatatagttc cttataacta gtttgatagt
      781 cctttatatc tatcaatact ccttgggaag cctccgccat acggcaggag gcattttttt
      841 gccgccgcaa aagctcaaaa acatttgcag gtcatgccgt ctgaatccga aacagcatta
      901 cctacaccgc catctaaaga caaccctgct acaatacgcc ttttattgtc cacgccgatt
      961 ttgccatgac cgagccgacc tacatccccc tgcgcctgca taccgagttt tcgattaccg
     1021 acggtatggt gcggattaaa aaactgattg ccaaagcgca ggaatacggt ttgcctgctt
     1081 tgggcatcag cgatttgatg aacgaattcg gcttggtgaa attctataaa gcctgccgca
     1141 gcgcaggaat taagcccatc ggcgcggcgg acgtgtggat aggcaatccg aatgcgcccg
     1201 acaagccgtt ccgtgccatg ctgattatcc gcaacgatgc gggctatctg cgtttgagcg
     1261 agcttttgac agaggcctat gtcggcaaag accgcaatat ccatcatgcg gaactcaacc
     1321 ccgaatggct ggaaaacggc gacaacagcg gcttgatttg tttgagcggc gcgcattacg
     1381 gcgaagtggg cgtgaatctg ttgaacggca atgaagacgc ggcgcgtgcg gcggcgttga
     1441 aatatgcggc gtggttcccc gatgcgttct atatggagct gcaacgccta cccgaacgcc
     1501 ccgaatggga ggcttgcgtt tcgggcagcg tgaagctggc ggaggaattg ggtttgccgg
     1561 tggtggcgac gcatccgaca cagtttatga gccgcgacga tttcaacgcg cacgaggcgc
     1621 gggtatgcat cgcaggcggc tgggtgctga cggacaagaa acgtccgcgc gatttcacgc
     1681 cggaccagtt tttcattccg ccggaaacga tgctggagcg ttttgccgat ttgcccgagg
     1741 ccttggaaaa cacggtggaa attgccaaac gctgcaacct gcacatcaca ttgggcaaaa
     1801 acttcctgcc ccttttcccc acgcccgacg gtttatcgcg cgatgactat ctcatcaaac
     1861 tctcaaacga gggtttgcag gaacgcatgg ttcagcttta tcccgacgag gcggagcgtg
     1921 cggcaaaaat gccggaatat caggaacgtt tggattttga gctgaacatc atcatccaga
     1981 tgaaattccc cggctatttc cttatcgtac aagactttat caactgggcg aaaacgcacg
     2041 gctgtccggt cgggccggga cgcggttcgg gcgcgggttc gctggtggcg tattcgctga
     2101 aaattaccga ccttgatccg ctcaaatacg cgctgctgtt tgaacgtttc ttaaaccctg
     2161 aacgcgtctc catgcccgac tttgacgtgg acttctgtca ggcaaaccgc ggccgcgtga
     2221 tcgaatatgt gcgcgataaa tacggcgctc aggcggtcag ccagattgtt accttcggca
     2281 cgatgtcgtc caaagcggtc atccgcgacg tggggcgcgt gttggagctg ccgtttatgc
     2341 tgtgcgacaa actgtccaag ctgattccgt tggaagccaa caaacccctg agtttggacg
     2401 acgcgatgaa ggcggagccg cagattcagg aattaatcga agcggaagaa gcggacgaac
     2461 tgattacgct ggcgaaaaag ctggaagacc tgacgcgcgg tttgggtatg cacgcaggcg
     2521 gcgtgttgat tgcgccgggc aagatttccg attacagccc cgtgtatcag gcggatgaat
     2581 ccgcctctcc cgtatccatg tacgacaagg gcgacgtgga agacgtgggt ttggtgaaat
     2641 tcgacttttt aggtctgcgc aacctgacca ttatcgaaat ggcacagaac aacatcaaaa
     2701 acaccaccgg cgacatcatc gatgtcggca aaatcccgct tgacgaccag gccgcctacc
     2761 aaatcttccg cgacgcgaac accaccgccg tcttccagtt cgagtcgacc ggcatgaaaa
     2821 aaatgctgaa aacggcgcac accaccaagt ttgaagaact catcgccttc gtatcgctct
     2881 accgacccgg cccgatggac aatattcccg acttcgtcgc gcgcatgaaa ggccaggaat
     2941 tccaatacat ccacccgctg ctcgaaggca tcctcgcgcc gacttacggg attatggtgt
     3001 atcaggaaca agtaatgcag gcggcgcaaa ttatcggcgg ctactcgctc ggcggcgcgg
     3061 acttgctgcg ccgcgccatg ggtaagaaaa aacccgaaga aatggtgaaa caccgcgaaa
     3121 tcttcgccga aggcgcggcg aaacaaggca tttcgcgcga aaaatccgac gaaatcttca
     3181 actatatgga aaaattcgcc ggctacggtt tcaacaaatc ccacgccgcc gcctacgccc
     3241 tgatttccta ccagaccgca tggctcaaag cccactaccc cgccgaattt atggcggcga
     3301 ccatgtcgtc cgaattggac aacaccgacc agctcaagca tttctacgac gactgccgtg
     3361 ccaacggcat cgagttcctg ccgcccgaca tcaacgaatc agactaccgc ttcacgccgt
     3421 atccgaacat gaaaatccgc tacgcgctcg gcgcgattaa aggcacgggc gaagccgccg
     3481 tcgaatccat catcgccgcg cggcaaagcg gcggcaaatt taccggcctc ttggacttct
     3541 gcgagcgcgt cggcaaagaa cacatgaacc gccgcaccct cgaagccctg atacgcggcg
     3601 gcgcgttcga cagcatcgaa cccaaccgcg ccatgctctt ggcaaacatc gacctcgcta
     3661 tggacaacgc cgaccaaaaa gccgccaacg ccaatcaggg cgggctgttc gatatgatgg
     3721 aagacgccat cgaaccggtg cggctcgtcg atgcacccat gtggagcgaa tcggaaaaac
     3781 tcgccgaaga aaaaaccgtc atcggctttt acctctccgg ccacccgttc ggcccgtatg
     3841 cccaagaagt ccgccaaatc gcaccgacca aattagaccg tctgaagccg caagacagcg
     3901 tgcgcctcgc cggattcgtt accgccgtgc gtacgatgat gggcaagcgc ggcaaaatcg
     3961 ccttcgtcag ccttgaagat ttgagcgggc aggttgaaat catggtcggc ggtcagacgc
     4021 tggaaaactg cgccgactgc ctcaaagccg accaagtgct gattatcgaa tccaaagtca
     4081 gccgcgacga ctacggcggc ggcgacgggc tgcgtattct ggcaaaccaa gtcatgaccc
     4141 tgcaaacggc gcgcgaacgc tacgcccgca gcctcagcct cgccctcgcc ccgcatcacg
     4201 acatcggcgg actggtacaa ctgcttgccg cccaccaact gcccgacacg ccgcgcatcc
     4261 cgctgcaact gtcgtatgcc aacggaaaag cgtcgggcag gtttcaagtg ccgccgaaat
     4321 ggacggttac accgagttcc gcattgttcg acgaactgga aaaactgctc ggcagccggt
     4381 cggtgcgcgt gaattggtaa cccaaaatat aaatgccgtc tgaagcccaa aatcggtttc
     4441 agacggcatt ttttaaccga aataaacgat gccccttaaa gcagaagctt taaggggcag
     4501 agcgttgcgg cacatctttt cagacggcct tattgtagca acggttctca tttcgcagtg
     4561 cgacaatatg tttgggattc tagccgttgt gagatgataa gtcaataatc tatttgtata
     4621 taaataagac aataagatat attatcatta tttgcaacgt tgtttacgga gtaataaatg
     4681 gctgccttca aacctaatcc aatcaactac atcctcggcc tcgatatcgg catcgcatcc
     4741 gtcggctggg cgatggtaga aattgacgaa gatgaaaacc caatctgcct gattgattta
     4801 ggcgtgcgcg tatttgagcg cgcagaagta ccgaaaacag gtgactctct cgcagcagca
     4861 agacgcttgg cacgcagcgt ccgccgcctg acccgccgtc gcgcccaccg cctgcttcga
     4921 gctcgtcgcc tattaaaacg cgaaggcgta ttacaagccg ctgattttga cgaaaacggc
     4981 ttgatcaaat ccttaccgaa tacaccatgg caacttcgcg cagccgcatt agaccgcaaa
     5041 ctgacgcctt tagagtggtc ggcagtcttg ttgcatttaa tcaaacaccg cggttatttg
     5101 tcgcaaagaa aaaacgaggg cgaaactgcc gataaagagc ttggcgcttt gctcaaaggc
     5161 gtagccgaca atgcccatgc cttacagaca ggcgatttcc gcacaccggc cgaattggct
     5221 ttaaataaat ttgagaaaga aagcggccat atccgcaatc agcgcggcga ttattcgcat
     5281 acgttcagcc gcaaagattt acaggcggag ctgattttgc tgtttgaaaa acaaaaagaa
     5341 tttggcaatc cgcatgtttc aggcggcctt aaagaaggta ttgaaaccct actgatgacg
     5401 caacgccctg ccctgtccgg cgatgccgtt caaaaaatgt tggggcattg caccttcgaa
     5461 ccggcagagc cgaaagccgc taaaaacacc tacacagccg aacgtttcat ctggctgacc
     5521 aagctgaaca acctgcgtat tttagagcaa ggcagcgagc ggccattgac cgataccgaa
     5581 cgcgccacgc ttatggacga gccatacaga aaatccaaac tgacttacgc acaagcccgt
     5641 aagctgctgg gtttagaaga taccgccttt ttcaaaggct tgcgctatgg taaagacaat
     5701 gccgaagcct caacattgat ggaaatgaag gcctaccatg ccatcagccg tgcactggaa
     5761 aaagaaggat tgaaagacaa aaaatcccca ttaaaccttt ctcccgaatt acaagacgaa
     5821 atcggcacgg cattctccct gttcaaaacc gatgaagaca ttacaggccg tctgaaagac
     5881 cgtatacagc ccgaaatctt agaagcgctg ttgaaacaca tcagcttcga taagttcgtc
     5941 caaatttcct tgaaagcatt gcgccgaatt gtgcctctaa tggaacaagg caaacgttac
     6001 gatgaagcct gcgccgaaat ctacggagac cattacggca agaagaatac ggaagaaaag
     6061 atttatctgc cgccgattcc cgccgacgaa atccgcaacc ccgtcgtctt gcgcgcctta
     6121 tctcaagcac gtaaggtcat taacggcgtg gtacgccgtt acggctcccc agctcgtatc
     6181 catattgaaa ctgcaaggga agtaggtaaa tcgtttaaag accgcaaaga aattgagaaa
     6241 cgccaagaag aaaaccgcaa agacagggaa aaagccgccg ccaaattccg agagtatttc
     6301 cccaattttg tcggagaacc caaatccaaa gatattctga aactgcgcct gtacgagcaa
     6361 caacacggca aatgcctgta ttcgggcaaa gaaatcaact taggccgtct gaacgaaaaa
     6421 ggctatgtcg aaatcgacca tgccctgccg ttctcgcgca catgggacga cagtttcaac
     6481 aataaagtac tggtattggg cagcgaaaac caaaacaaag gcaatcaaac cccttacgaa
     6541 tacttcaacg gcaaagacaa cagccgcgaa tggcaggaat ttaaagcgcg tgtcgaaacc
     6601 agccgtttcc cgcgcagtaa aaaacaacgg attctgctgc aaaaattcga tgaagacggc
     6661 tttaaagaac gcaatctgaa cgacacgcgc tacgtcaacc gtttcctgtg tcaatttgtt
     6721 gccgaccgta tgcggctgac aggtaaaggc aagaaacgtg tctttgcatc caacggacaa
     6781 attaccaatc tgttgcgcgg cttttgggga ttgcgcaaag tgcgtgcgga aaacgaccgc
     6841 catcacgcct tggacgccgt cgtcgttgcc tgctcgaccg ttgccatgca gcagaaaatt
     6901 acccgttttg tacgctataa agagatgaac gcgtttgacg gtaaaaccat agacaaagaa
     6961 acaggagaag tgctgcatca aaaaacacac ttcccacaac cttgggaatt tttcgcacaa
     7021 gaagtcatga ttcgcgtctt cggcaaaccg gacggcaaac ccgaattcga agaagccgat
     7081 accccagaaa aactgcgcac gttgcttgcc gaaaaattat catctcgccc cgaagccgta
     7141 cacgaatacg ttacgccact gtttgtttca cgcgcgccca atcggaagat gagcgggcaa
     7201 gggcatatgg agaccgtcaa atccgccaaa cgactggacg aaggcgtcag cgtgttgcgc
     7261 gtaccgttaa cacagttaaa actgaaagac ttggaaaaaa tggtcaatcg ggagcgcgaa
     7321 cctaagctat acgaagcact gaaagcacgg ctggaagcac ataaagacga tcctgccaaa
     7381 gcctttgccg agccgtttta caaatacgat aaagcaggca accgcaccca acaggtaaaa
     7441 gccgtacgcg tagagcaagt acagaaaacc ggcgtatggg tgcgcaacca taacggtatt
     7501 gccgacaacg caaccatggt gcgcgtagat gtgtttgaga aaggcgacaa gtattatctg
     7561 gtaccgattt acagttggca ggtagcgaaa gggattttcg ccggataggg ctgttgtaca
     7621 aggaaaagat gaagaagatt ggcaacttat tgatgatagt ttcaacttta aattctcatt
     7681 acaccctaat gatttagtcg aggttataac aaaaaaagct agaatgtttg gttactttgc
     7741 cagctgccat cgaggcacag gtaatatcaa tatacgcatt catgatcttg atcataaaat
     7801 tggcaaaaat ggaatactgg aaggtatcgg cgtcaaaacc gccctttcat tccaaaaata
     7861 ccaaattgac gaactgggca aagaaatcag accatgccgt ctgaaaaaac gcccgcctgt
     7921 ccgttaaatt aatctatccc tgtttcaggc ggcctgaagc agggattttt tatataaaaa
     7981 taaggggaaa aacatgactt ggcgcagcct gcttattcaa aacggcggca aactatcctt
     8041 gcaacgccgg caactgctga ttcaacaaaa cggcgagtcc cacaccgtac cattagagga
     8101 catcgccgtt atcatcatcg aaaatcgcga aaccctgatt accgccccgc tcctatcagc
     8161 cttagccgag catggtgcaa ccctgctgac ctgcgacgag cagttcctgc cctgcggaca
     8221 atggctgcct tatgcccaat atcaccgcca acttaaaatc ctcaaactgc aactgaacat
     8281 aagcgaaccc ttaaaaaagc aactctggca acatatcgtc cgccaaaaaa tcctgaacca
     8341 agcatttgtc gcagacgaaa caggcaatga tcttgcagcc aaacggctgc gcactttggc
     8401 atctgaagtc cgttcaggcg atacaggaaa ccgtgaagcc caagctgccg ccctctactt
     8461 tcaagccctc tttggagaaa aatttacccg taatgacaac aacgccgtca atgccgcatt
     8521 gaactacacc tacgccgtac tccgtgccgc agttgcacgc gccctgacct tatacggttg
     8581 gttacctgca ttaggcttgt ttcatagaag tgaactcaat ccatttaatc tcgccgatga
     8641 ctttatcgaa cccttacgcc cattggccga cctgaccgtg atacatttgt acgaacaagg
     8701 ccgtctgaaa actgaactga cgctcggcat caaacaacac ctcatcaaaa tcctctacta
     8761 tcaaaccagc atcgaacggc aacatttcag caccttggct gccatcgaca aaatgatttc
     8821 gtcatttcaa gccggcgtaa ccgataaaaa cgccaaacaa ctgaaactgc ctgaaatcct
     8881 accattgaaa gaataccaat atgagtgagg ccaaatttat gaggattatc gtcttcttcg
     8941 acctaccggt tatcacggcg gcaaagcgca aagccgccaa tcaattccgc cagtttttat
     9001 taaaagacgg ataccaaatg ctgcaacttt ccgtatacag ccgtatcgtc aaaggccgcg
     9061 attcgttgca aaaacaccac aacagactgt gtgcaaacct tccgcaagaa ggctcaatcc
     9121 gctgtttgga gataacagaa aagcaatatg ccgccatgaa actgctgttg ggcgagctga
     9181 aaacccaaga aaaaaaggtc aattcagacc aattattgtt attttaagcc cattttttca
     9241 gactaaataa aacgggaaat ccttatagaa taaggatttc ccgttggggt attgtagcac
     9301 tgcgaaatga gaatgggagc tacaacaaag tgttgggtca ggagaccgtt gcattaattg
     9361 tagcactgcg aaatgagaat gggagctaca accgcgcacg tcgaaatatt gttcgcccac
     9421 gcattgtagc actgcgaaat gagaatggga gctacaacgc gacgcgctcg cctattctgc
     9481 cggaaaagat tgtagcactg cgaaatgaga atgggagcta caacgctcat gaacagcgag
     9541 ttcaaaaaag gcttattgta gcactgcgaa atgagaatgg gagctacaac ggatttttcc
     9601 gaccccctaa aaagtttttg attgtagcac tgcgaaatga gaatgggagc tacaaccgtt
     9661 ctgatggcaa tttataaaaa aatattattg tagcactgcg aaatgagaat gggagctaca
     9721 acgtattttt tcccgacccc ctaaaaagtt ttattgtagc actgcgaaat gagaatggga
     9781 gctacaaccc ctgtcggctg cgtcgatggc ggcaaaagat tgtagcactg cgaaatgaga
     9841 atgggagcta caacctatgc cgggtatctc atcgcgctgg aaaaattgta gcactgcgaa
     9901 atgagaatgg gagctacaac gaagacgaaa gacgtattaa tagaacctat attgtagcac
     9961 tgcgaaatga gaatgggagc tacaacttca agatgtattc attgttccaa ctgagcattg
    10021 tagcactgcg aaatgagaat gggagctaca accgcccgag attttcgcca ttggtataat
    10081 ttattgtagc actgcgaaat gagaatggga gctacaacaa acccaagcga tgtaattgca
    10141 taccccatat tgtagcactg cgaaatgaga atgggagcta caactaacaa cagataaacg
    10201 tgtttatagc tttgattgta gcactgcgaa atgagaatgg gagctacaac gtatttttcg
    10261 acccccctaa aaagtttttg attgtagcac tgcgaaatga gaatgggagc tacaaccttg
    10321 ttaagcaagt taaacgtgct aagtctattg tagcactgcg aaatgagaat gggagctaca
    10381 acacgcatgg ggttttcatg gtattgatt
//
LOCUS       DAXHSF010000059        10215 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.059, whole genome shotgun sequence.
ACCESSION   DAXHSF010000059 DAXHSF010000000
VERSION     DAXHSF010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10215
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.059"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..144
                     /locus_tag="ACFM8S_001073"
     CDS             <1..144
                     /locus_tag="ACFM8S_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HFC6314152.1"
                     /translation="NVGIGSGALLGHWVTQYVGISRIGIAGMLAAAAGLWLCLNLNRH
                     IQT"
     gene            274..1710
                     /locus_tag="ACFM8S_001074"
     CDS             274..1710
                     /locus_tag="ACFM8S_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HFC6314153.1"
                     /translation="MPLLSVEFALFFLAFLPIYWGLAKYPSVQNLLLLAAGMGWLYHI
                     GPVFAAIVVLYSSCVYLLGELLRSDRENTRRFWLGCGIAVSLTVLGFFKYFDFFRPLI
                     AQYAGQGGAIDILMPLGLSYYTFQSIAYLVYCFRAPHAARFEWHELLLHLSFFPTVTS
                     GPIIRAAAFKSADGEQAGALAQIRTHQPRSPVRPALAVSLILLGIAKKWWLAGMLAEN
                     WVSPVFENPAQFDGWGVLAGVYGYTFQLFLDFSGYSDLVIGMAMLLGFRLPKNFSAPL
                     RAANIRAFWDKWHISLSTWIRDYIYIPLGGSKKGFLRTQFNLMAAMVLSGIWHGYGWN
                     FLIWGALHGTALVLLNTGDRYFGRDALCRLKYLAPLSWFVTFHFVCLSFVVFNTANPD
                     DAGAVFSALFANASGWNAPQQADMLLLASFASVMLLYPYLQRAFDGVVKGLEKIPMWL
                     WFIPVSAVLLLIIVLAPSGIPGFIYANF"
     gene            1721..2704
                     /gene="patB"
                     /locus_tag="ACFM8S_001075"
     CDS             1721..2704
                     /gene="patB"
                     /locus_tag="ACFM8S_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan O-acetyltransferase PatB"
                     /protein_id="HFC6314154.1"
                     /translation="MKNFLSLFASILMSALIAVWFSQNPINAYWQQTYHRNSPLEPLA
                     AYGWWRSGVALQENAYALSDGIKAFLSGETPPTAQDGGSADMPSEAAASEAVPQTGET
                     EWKQDTEAAAVRSGDKVFFAGDSLMQGVAPFVQKSLKQQYGIESVNLSKQSTGLSYPS
                     FFDWPKTIEETLKKHPEISVLAVFLGPNDPWDFPVGKRYLKFASDEWAQEYLKRVDRI
                     LEAAHTHRVQVVWLGIPYMKKAKLDGQMRYLDKLLSEHLEGKIILIPTAHTLSGGEDR
                     YTDSVNVNGKPVRYRSKDGIHFTAEGQKLLAAKIMEKIVFEPSTQPSSTQP"
     gene            2701..3894
                     /locus_tag="ACFM8S_001076"
     CDS             2701..3894
                     /locus_tag="ACFM8S_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFC6314155.1"
                     /translation="MNPKHLIAFSALFAATQAEALPVASVSLDTVTVSPSAPYTDTNG
                     LLTDYGNAAASPWMKKIRSVAQGSGETFRILQIGDSHTAGDFFTDSLRKRLQKTWGDG
                     GIGWVYPANVKGQRMAAVRYNGNWQSLTSRNNTGDFPLGGILAHTGSGGSMTLTASDG
                     KTGKQRISLFAKPLLAEQTLTVNGNTVSANGGGWQVLDTRAALPLTIQTEMPWDIGFI
                     NIENPAGGITVSAMGINGAQLTHWSKWRADRMNDLAQTGADLVILSYGTNEAFGDNID
                     IADTEQKWLDTVRQIRDSLPAAGILIIGAPESLKNTLGVCGTRPVRLTEVQQMQRRIA
                     RQGQTMFWSWQNAMGGICSMKNWLNQGWAAKDGVHFSAKGYRRAAEMLADSLEELVRA
                     AAIRQ"
     gene            complement(3954..4130)
                     /locus_tag="ACFM8S_001077"
     CDS             complement(3954..4130)
                     /locus_tag="ACFM8S_001077"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314156.1"
                     /translation="MPSENGRRQFIIFAFRRHIKRRLCAVCRRFAPVLPMLEANAGCV
                     RQTVRGVAMPSEAI"
     gene            4181..5734
                     /locus_tag="ACFM8S_001078"
     CDS             4181..5734
                     /locus_tag="ACFM8S_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HFC6314157.1"
                     /translation="MNRTYANFYGMLAAACRKNGNGTAVFDGKEKTAYRALKQEAEAV
                     AAYLQNIGVKFGDTVALAVSNSTEFITAYFAVSAIGAVAVPMNTFLKNSEYAYILNDC
                     KARFLFASAGLSKELAGLKAQTPVEKIIWTGQSRPDGEMAEGDAFFEDVRRFPEKPDL
                     GRQPRINDLAHIIYTSGTTGHPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMF
                     HSFTLTAMVLLPIYMACSIILVKSVFPFSNVLKQTLLKRATVFLGVPAIYTAMSKAKI
                     PWYFRWFNRIRLFISGGAPLAEQTILDFKAKFPRAKLLEGYGLSEASPVVAVNTPERQ
                     KARSVGIPLPGLEAKAVDEELVEVPRGEVGELIVRGGSVMRGYLNMPAATDETIVNGW
                     LKTGDFVTIDEDGFIFIVDRKKDLIISKGQNVYPREIEEEIYKLDAVEAAAVIGVKDR
                     YADEEIVAFVQLKEGMDLGENEIRRHLRTVLANFKIPKQIHFKDGLPRNATGKVLKRV
                     LKEQFDGNK"
     gene            complement(5825..7852)
                     /locus_tag="ACFM8S_001079"
     CDS             complement(5825..7852)
                     /locus_tag="ACFM8S_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HFC6314158.1"
                     /translation="MSLSEFIERRTSFNPMVILTTLFFVCVLVVLVLTVPDQVQMWLD
                     RAKEVIFTEFGWFYVLTFSIFLGFLLILSVSSLGNIRLGRDEDVPEFGFLSWLAMLFA
                     AGMGVGLMFFGVAEPLMHYFSDITAGTPEHRQQQALLHTVFHWGVHAWSVYGTIALAL
                     AYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDIMALLATFFGIITTLGFGASQLGA
                     GLQEMGWIAENSFSVQVLIIAAVMSLAVVSAISGVGKGVKVLSELNLSLAFLLLFFVL
                     AAGPTVYLLSAFGDNIGNYLGNLVRLSFKTYAYEREHKPWFESWTVLYWAWWCSWAPF
                     VGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTAIWLNDGVAGGMLEKMTSS
                     PETLLFKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNNITSRDKGLSAPRWQA
                     VMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSLWKGLSADKKYFET
                     RVNPTSVFWTGGKWKERLVQIMSQTQEQDILKFLKHTASPAMHELQRELSEEYGLSVR
                     VDKMFHQDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGKLPHIRHQTTYKPYA
                     YFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE"
     gene            8141..10147
                     /locus_tag="ACFM8S_001080"
     CDS             8141..10147
                     /locus_tag="ACFM8S_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HFC6314159.1"
                     /translation="MADMKKITPQNLRPLLSESLGHTDFVNVLNALIKFLRRGGKKCA
                     GERFDLIIDTFKQDRELLSRFSRCFYIWLAQIHIYPALIKLGIFSRHSFAREMGIRIY
                     ERFSPSYKDFANLGEVFLYLFHSENDDKWLQTLNIRQWLVLYELIRSHAEPSKLQTAG
                     IRLADARLRAIEMLSVWTASEAIEPDLIRIAPRLLEADSSFVALQRETAKLVEHYRNG
                     TTPYDTAHLEVMFDQCFSQIDYLRRKGTGAGSGSSVKVAHLLERLRQTVDRLKLLTDI
                     QTGAGNSNRLTIALMNSLIYAAVEQYSTRHLRRSSIRMLARSITENKSHHGEHYITRN
                     RKEYFKMFYSAAGGGIIIALMALLKIRIGTLGLSPFLTSLSAGFNYGIGFMIIHMLHC
                     TVATKQPAMTAASFAEQVDLNEGGKAVDNKLAKLLIDVCRSQSVAVFGNVSIAILLAC
                     AISFGYAHLHQQPILDAHTAAYQFKSIDIIAYPTLWYAAIAGLWLFCSGIIAGFFDNR
                     ADYLNLRQRLPFNPLLRKIMRPGPRRVLAAYIHKHYGSLAGNFIFGMLLGMTGYFGHL
                     LGLPLDIRHVAFSSANLGYAAVSGNVGLGTFVLGIFSVLAIGLVNLCVSFSLALFVAL
                     RSRGTKIGSIRNLIKSFWNQIKSNPCILFLPPAKEQGHPPSDKP"
BASE COUNT         2439 a         3209 c         2471 g         2096 t
ORIGIN      
        1 aacgtcggta tcggcagcgg cgcactgttg ggacattggg taacgcaata tgtcggcatt
       61 tcccgtatcg gcatcgccgg tatgcttgcg gcggcggccg ggctgtggct gtgcctgaac
      121 ttgaaccgcc atatccaaac ctgaccgcgc gatgccgtct gaagcccctc ccgcccttca
      181 gacggcattt tgattgaaga aatatccgcc cctgcttaaa ataatgggct ttgccgtgtt
      241 ttaacgccta ttttcttatt ccaaggatgg tttatgccgc tgctgtctgt cgagttcgca
      301 ctgttctttc tcgccttcct gccgatttac tggggcttgg cgaaataccc gtccgtccaa
      361 aacctgctgc ttttggctgc cggtatgggc tggctctacc atatcggccc cgtatttgcg
      421 gcaatcgtcg tcctttattc ctcctgcgtg taccttttgg gcgaactgct ccgttccgat
      481 cgcgaaaata cgcgccgctt ttggctgggg tgcggcattg ccgtctcgct gaccgtcttg
      541 ggctttttca aatatttcga ttttttccgc ccgctgattg cacaatatgc cggacaaggc
      601 ggcgcaatcg acatcctgat gccgctgggg ctttcgtatt acaccttcca gtcaatcgcc
      661 tatttggttt actgcttccg tgcgccgcac gccgcgcgtt tcgagtggca cgaactgctg
      721 ctgcacctga gttttttccc caccgttacc tccggcccga ttatccgcgc cgccgcattc
      781 aaaagcgcgg acggcgagca ggcgggcgca ttggcgcaaa tccgtaccca ccaaccacgc
      841 tcacccgtcc gccccgcact cgccgtttcc ctgattctgc tgggtattgc caaaaaatgg
      901 tggctggcgg ggatgctcgc agaaaactgg gtatcgcccg tatttgaaaa tcccgcccaa
      961 ttcgacggct ggggcgtatt ggcgggcgtg tacggctata ccttccaact ctttttagac
     1021 ttttccggat attccgactt ggttatcgga atggcaatgc tgctgggctt ccggctgccc
     1081 aaaaacttct ccgcaccgct tcgtgccgcg aatatccgcg cattttggga caaatggcac
     1141 atcagccttt ccacctggat acgcgactac atctacatcc ccttgggcgg cagcaaaaaa
     1201 ggctttttgc ggacacagtt caacctgatg gcggcaatgg tactctccgg catatggcac
     1261 ggctacggct ggaacttcct catttggggc gcgctgcacg gcacggcgct ggtgctgctc
     1321 aacacgggcg accgttattt cggacgcgac gcgctatgcc gtctgaaata ccttgcgccg
     1381 ctctcgtggt ttgttacctt ccattttgtc tgccttagct ttgtcgtctt caataccgca
     1441 aaccccgacg atgccggcgc ggttttcagc gcgctctttg ccaatgccag cggctggaat
     1501 gcgccgcaac aggcagacat gctgctgctt gcctcgtttg catccgtgat gctgctctac
     1561 ccttacctgc aacgcgcttt cgacggcgtg gtcaaaggtt tggaaaaaat cccgatgtgg
     1621 ctgtggttta tccccgtttc cgccgtcctg ctgctgatta tcgtcctcgc cccctcgggg
     1681 atacccggct ttatttatgc caatttttaa gggtttggac atgaaaaact ttctttccct
     1741 tttcgcctcc atattgatgt ctgccctgat tgccgtgtgg ttcagccaaa accccatcaa
     1801 cgcctactgg cagcagacct accaccgcaa cagcccgctc gaaccgctcg ccgcctacgg
     1861 atggtggcgg agcggtgtgg cgttgcaaga aaacgcctac gccctttcag acggcatcaa
     1921 agccttcctg tccggcgaaa cgccgccgac ggctcaagac ggcggttcgg cagatatgcc
     1981 gtctgaagcc gccgcatcag aagccgtccc tcaaaccggt gaaacagaat ggaaacaaga
     2041 caccgaagcc gccgccgtcc gcagcggcga caaagtcttt tttgccggcg actcgctgat
     2101 gcagggcgtt gcacccttcg tgcaaaaaag cctgaaacag caatacggca tcgaatccgt
     2161 caacctcagc aaacaaagca cggggctgtc ctacccctca ttcttcgact ggccgaaaac
     2221 gattgaagaa accctgaaaa aacatcccga aatcagcgtg ctcgccgtct tcctcggccc
     2281 gaacgacccg tgggatttcc ccgtcggcaa acgatacctc aaattcgctt ccgacgaatg
     2341 ggcgcaagaa tacctgaaac gcgtcgaccg catccttgaa gccgcacaca cgcaccgcgt
     2401 ccaagtcgtc tggctcggca tcccctatat gaaaaaagcc aagctcgacg ggcagatgcg
     2461 ctacctcgac aaactgcttt cggaacacct ggaaggcaaa atcatcctga ttcccaccgc
     2521 gcacaccctg agcggcgggg aagaccgcta caccgactcc gtcaacgtca acggcaaacc
     2581 cgtccgctac cgcagcaagg acggcataca ctttaccgcc gaaggacaaa aactgctggc
     2641 ggcaaaaata atggaaaaaa tcgtttttga accaagtacg caaccatcaa gtacacagcc
     2701 atgaacccca aacacctcat cgcattttcc gccctgttcg ccgccacgca ggcagaagcc
     2761 ctgcccgtcg cctccgtcag cctcgacacc gttaccgttt ccccgtccgc cccctacacc
     2821 gatacaaacg ggctcttgac cgactacggc aacgccgccg cctcgccttg gatgaaaaaa
     2881 atccgatccg tcgcacaagg cagcggcgag accttccgca tcctgcaaat cggcgactcg
     2941 cataccgccg gcgacttctt taccgacagc ctccgcaaac gcctgcaaaa aacttggggc
     3001 gacggcggca taggctgggt ttaccccgcc aacgtcaaag gacagcgtat ggcggccgtc
     3061 cgctataacg gcaactggca aagcctcacc agcaggaaca acaccggaga cttcccgctc
     3121 ggcggcatcc tcgcccacac cggcagcggc ggcagcatga ccctgaccgc atcggacggc
     3181 aagaccggca aacagcgcat ctccctgttt gccaaacccc tgctcgccga acaaaccctg
     3241 accgtcaacg gcaacaccgt ctccgccaac ggcggcggct ggcaggtact ggatacccgc
     3301 gcggcactgc ccttgaccat acagaccgaa atgccgtggg acatcggctt catcaacatc
     3361 gaaaatcccg ccggcggcat taccgtttcc gccatgggca tcaacggcgc acaattgacc
     3421 cattggtcga aatggcgtgc cgaccgtatg aacgaccttg cccaaaccgg cgccgatttg
     3481 gttatccttt cctacggcac caacgaagcc ttcggcgaca acatcgacat tgccgatacc
     3541 gaacagaaat ggctggatac cgtccgccaa atccgcgaca gcctacctgc cgccggcatc
     3601 ctcatcatcg gcgcgcccga atccctgaaa aacacgctcg gcgtatgcgg cacacgcccc
     3661 gtccgcctga ccgaagtcca acagatgcag cggcgcatcg cccggcaggg acaaaccatg
     3721 ttctggtctt ggcaaaacgc catgggcggc atatgcagca tgaaaaactg gctcaaccaa
     3781 ggatgggccg ccaaagacgg cgtacacttc tccgccaaag gctaccggcg cgcggcggaa
     3841 atgctcgccg acagcctcga agaactcgtc cgcgccgctg caatcaggca ataatcggac
     3901 aggaggcgga cggtatttcc gcaacagggg gatgccgtct gaaacgcata ccttcatatt
     3961 gcttcagacg gcatagccac cccgcgcacg gtttgccgga cgcaaccggc attcgcctca
     4021 agcatcggaa ggacgggggc gaacctccgg catacggcgc aaaggcggcg tttgatatgc
     4081 cgtctgaagg caaagatgat aaactgccgc cttccgtttt cagacggcat attgttttca
     4141 aatgagggcg ttcgccgtcc gcaaccataa aggaagtttc atgaaccgga cttatgccaa
     4201 tttctacgga atgctcgccg ccgcctgccg caaaaacggc aacggcacgg cagtgttcga
     4261 cggcaaggaa aaaaccgcct accgcgcgct caagcaggaa gccgaagccg ttgcggcgta
     4321 tctgcaaaat atcggcgtga agttcggcga cacggtcgcg ctggcggttt ccaattccac
     4381 ggaatttatt accgcctatt tcgccgtatc cgccatcggc gcggttgccg taccgatgaa
     4441 cacatttttg aaaaacagcg aatacgcgta tatcctgaac gactgcaagg cgcgcttcct
     4501 gttcgcctcg gccggcctgt caaaagaatt ggcgggcttg aaggcgcaaa cgcccgtcga
     4561 aaaaatcatt tggacgggcc aaagccgtcc ggacggcgaa atggcggaag gcgatgcctt
     4621 ttttgaagac gtgcgccgct tccccgaaaa acccgacttg ggccgccaac cccggataaa
     4681 tgatttggca cacatcatct acacctccgg cacgacgggg catcccaaag gtgcgctaat
     4741 cagttacgcc aacctgttcg ccaacctgaa cggcatcgaa cgcatcttta aaatttccaa
     4801 acgcgaccgc tttatcgttt tcctgccgat gttccacagc ttcacgctga cggctatggt
     4861 gctgctgccg atttatatgg cgtgttcgat tattttggtc aaatccgttt tcccgttttc
     4921 caatgttttg aaacagaccc tgctcaaacg cgcgaccgtg tttttgggcg tacccgcgat
     4981 ttacaccgcg atgagcaagg cgaaaatccc ttggtatttc agatggttca accgcatccg
     5041 cctgtttatc agcggcggcg cgcctttggc ggaacaaacc atcctcgatt tcaaagccaa
     5101 gttcccccgc gccaaattgc tggaaggcta cggactgagc gaagcctctc ccgtcgtcgc
     5161 cgtcaatacg cccgagaggc aaaaagcccg cagcgtcggc atccccctgc ccggtttgga
     5221 agccaaagcc gtcgatgaag aattggtcga agtgccgcgc ggcgaagtgg gcgaactgat
     5281 cgtcaggggc ggttcggtga tgcggggcta cctcaatatg cctgccgcca ccgatgaaac
     5341 catcgtcaac ggctggttga aaacgggcga tttcgttacc atagacgaag acggctttat
     5401 ctttatcgtc gaccgcaaaa aagatttgat tatttccaaa ggtcaaaacg tctatccgcg
     5461 cgaaatcgaa gaagaaatct acaaactcga tgccgtcgaa gccgccgccg tcatcggcgt
     5521 gaaagaccgt tatgccgacg aggaaatcgt cgccttcgtc caattgaagg aaggtatgga
     5581 tttgggcgag aacgaaatcc gccgccacct gcgtaccgtg ctggcaaatt tcaaaatccc
     5641 caaacaaatc cactttaaag acgggctgcc gcgcaacgct acgggcaagg tattgaaacg
     5701 ggtgttgaag gagcagtttg acggaaacaa atgaacgccg tgccgtccga agccccgtcc
     5761 ggcaaaaaaa atgcggtgaa tctgcttcac cgcatttttt tggcaaacgc cggagcgagg
     5821 atatttactc tgccaattcc acctgctcgt gcgccatcag ttcctgaccg acatcatcca
     5881 acaacatcaa ataacgttcg tagtttttca aaatgtcggc aatcagctcg tccttgttca
     5941 tatactgcac atcgtacccg acgcgcccgt cgaaaaaata agcgtagggt ttgtaagttg
     6001 tctgatgccg gatatgcggc agcttgccgt cgttaatcaa ctggtcggat acatcctgcc
     6061 cgacagactt aatcccgtac ataaaatcgc gcatcgtctc tttccgaatg acgaactcga
     6121 ttgcgggctc gtcctgatga aacatcttat cgacccggac gctcaagccg tattcttccg
     6181 aaagctcccg ttgcaactcg tgcatagcgg gcgatgcggt atgtttgagg aattttaaaa
     6241 tatcctgctc ctgcgtctgg ctcattatct gcaccagccg ttctttccac ttgccgcccg
     6301 tccaaaatac actggtaggg ttaacccggg tctcaaaata tttcttatcc gcactcaagc
     6361 ctttccacag gctgaaacac attatcagca tcagcagggc aaacggcagg gaaacaatca
     6421 gggtcataga ctgcaggttg ccgagtccgc ccgagcgcat cagcaaaacg gcaacggcag
     6481 acatcagcac gccccacata accgcctgcc accgtggcgc gctcaagcct ttgtcccgag
     6541 aggtaatatt gttcaggaca taaatcccgg aatcggcaga agttacaaaa aacagagaaa
     6601 tgaccagcag gctgacgatg ctcgtcaatt cgggcagggg gaggtaatta aagaatttaa
     6661 aaagcagcgt ttccggagag gaggtcatct tttcgagcat tccccccgca accccgtcat
     6721 tcagccaaat cgccgtattg ccgaagacgg taaaccacaa aacgccgaac aggccgggga
     6781 tgagcaaaac cccgaagaca aactcgcgga tggtgcgccc ctttgaaatg cgcgcgataa
     6841 acaaacccac aaacggcgcc caagaacacc accacgccca ataaagcacc gtccaagatt
     6901 caaaccacgg cttgtgttcc cgttcgtacg cataagtttt aaaactgagg cgcaccagat
     6961 ttccgaggta gttccctatg ttgtcgccga atgccgacaa caggtaaaca gtgggacccg
     7021 ccgccaaaac aaaaaacagc agcaaaaacg caaggctcag gttcaactcg ctcaacacct
     7081 tcacgccctt ccccacgccg gatattgccg aaacgacggc gagggacatg acggcggcga
     7141 taatcaaaac ctgcacgctg aagctgtttt cggcaatcca gcccatttcc tgcaatccgg
     7201 cgcccagttg cgaagccccg aaccccaatg tggtgatgat gccgaaaaaa gtagcaagca
     7261 acgccataat atcaatggca tcgccgaacc ttccggaaat tttttctttc aacaggggat
     7321 aaaaacaaga acgcagggca agcggcagct tgtagcggaa accgaaataa gccaaagcta
     7381 atgcaatcgt accgtacacc gaccaagcgt gaacgcccca atggaacacc gtgtgcagca
     7441 atgcctgctg ctgcctgtgt tccggcgtgc cggccgtaat gtccgaaaaa taatgcatca
     7501 acggctctgc cacgccgaaa aacatcagac ccacgcccat cccggccgca aacagcatcg
     7561 ccagccacga caggaagccg aattccggca catcttcatc ccgtccgagc ctgatgtttc
     7621 ccaaactgct gaccgagagt atcagcagga aacccagaaa aatggaaaac gttaaaacat
     7681 aaaaccagcc gaactcggta aaaatgactt cttttgcccg atcgagccac atctgcacct
     7741 gatccggcac ggttaaaacc aataccacca aaacacacac aaaaaacaaa gtcgtcaaaa
     7801 taaccatcgg attaaatgac gttcggcgtt ctataaattc agacagggac aaaccttctc
     7861 actcctttgt taaaaacaga caaacccggt catcgggcaa agcggtcaaa acctgccgac
     7921 aaaataccgg cttccggatg acgaaatgca caaaccggcc gaaattgtta taatcgaaaa
     7981 aattaaaaat caatacggat tattctatca gattgattta tcgatattta ttatatcatt
     8041 aatatagttt gatttcaaac cggcggaaaa tgcgccgcct tccgcaggcg cgccctcccc
     8101 gccatcgggg cgtttaacgc cggattgccg atgcggtaca atggctgata tgaagaaaat
     8161 tacccctcaa aacctgcgcc ccctgctttc ggaaagcttg ggacataccg attttgtcaa
     8221 cgtcctcaac gcactgatta aatttttgcg ccgtggcggc aaaaaatgtg cgggggagcg
     8281 tttcgacctg attatcgaca cattcaaaca agatagggaa ttgctgtccc gcttcagccg
     8341 gtgtttttac atctggctcg cgcaaataca catttatccg gcactcatca aactcggcat
     8401 cttctcgcgc cacagctttg cccgagaaat gggcatacgc atctacgaac gcttcagccc
     8461 gtcatataaa gattttgcca acttgggcga agtcttcctt tatcttttcc attccgaaaa
     8521 cgacgacaaa tggctgcaaa cgctcaatat ccgccaatgg ctggttttat acgaactcat
     8581 ccggagccac gccgagccgt ccaaattgca gacggcgggc atccgccttg ccgatgcgcg
     8641 tttgcgcgcc atcgaaatgc tgtctgtctg gacggcatcc gaagccatcg aacccgacct
     8701 catccgcatc gccccgcgcc tgctggaagc cgattcttcc ttcgtcgccc tccaacgcga
     8761 aaccgccaaa ctggtcgaac actaccgcaa cggcaccacg ccttacgaca ccgcccacct
     8821 cgaagtgatg ttcgaccaat gtttcagcca gattgactat ttgcgccgca aagggacggg
     8881 cgccggctcc ggttcgtcgg tcaaagtcgc ccacctgctc gaacggctcc ggcagaccgt
     8941 agaccgtcta aagctgctca ccgacatcca aaccggtgcc ggcaacagca accgcctgac
     9001 catcgcgctg atgaactccc tcatctacgc ggcggtcgaa caatacagca cccgccacct
     9061 gcgccgcagc agcatccgta tgctcgcccg cagcattacc gaaaacaaaa gccaccacgg
     9121 cgaacactac atcacccgca accgcaaaga atatttcaaa atgttctact cggcggcagg
     9181 cggcggcatc atcatcgccc tgatggcgct gctcaaaatc cgcatcggca cactcggcct
     9241 cagccccttc ctgacttcct tgtcggctgg gttcaactac ggcatcggct ttatgatcat
     9301 ccatatgctg cactgcaccg tcgccaccaa acagcccgcg atgactgccg ccagctttgc
     9361 cgaacaggtc gacctcaacg aaggcggcaa agcggtggac aacaaactcg ccaagctcct
     9421 catcgacgta tgccgctccc aaagtgtcgc cgtcttcggc aacgtttcca tcgccatcct
     9481 tttggcgtgc gccatatcgt tcggctatgc ccatctgcac caacagccca tactcgatgc
     9541 ccacaccgcc gcctaccagt tcaaatccat agacatcatc gcttacccga cgctgtggta
     9601 tgccgccatt gcaggtctgt ggctgttctg ctccggcatc atcgcaggct tcttcgacaa
     9661 ccgcgccgac tacctcaacc tgcgccaacg cctgcccttc aaccccttgc tgcgtaaaat
     9721 aatgcgcccc ggaccccgcc gcgtcctcgc cgcctacatc cacaaacact acggctcact
     9781 ggcaggcaac ttcatcttcg ggatgctctt gggtatgacc ggctatttcg gacacctcct
     9841 cgggctgccg ctggacatcc gccacgtcgc cttttcctcc gccaacctcg gctatgccgc
     9901 cgtcagcggc aacgtcggtt tgggcacgtt cgtactcggc attttcagcg tcctcgccat
     9961 cggcctggtc aacctctgcg tcagcttcag cctcgccctc ttcgtcgccc tgcgctcgcg
    10021 cggcacgaaa atcggcagca tccgcaatct gattaagagt ttttggaatc agattaaaag
    10081 caatccctgc atacttttcc tcccgcccgc caaagaacag ggacatcctc cttcggacaa
    10141 gccttgaccg gcaatgccgt ctgaagcggg attcgccccg aataccgccc tgatgcggga
    10201 aatccccata aaagg
//
LOCUS       DAXHSF010000060        10005 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.060, whole genome shotgun sequence.
ACCESSION   DAXHSF010000060 DAXHSF010000000
VERSION     DAXHSF010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10005
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.060"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(133..1806)
                     /gene="recN"
                     /locus_tag="ACFM8S_001081"
     CDS             complement(133..1806)
                     /gene="recN"
                     /locus_tag="ACFM8S_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFC6314160.1"
                     /translation="MLLTLSLRDFVIVENLNLDFQSGFTVLTGETGAGKSITLDAIGL
                     LLGDKADYSQVRSGAKEAQLSALFDISHLPVLKAELYEQGLLNDGEEELSIRRIIDAK
                     GKSRSFINNQAATLAQLKAVGSQLIDIHGQNAHHSLNQEAAQRELLDAFAGSRAQAET
                     VRQLYQNWANSKKALQEAQEQAENIALEQERLEWQFNELNQLDIKQGEWEALSQSHDS
                     LAHSAELLQAAEEVGSKIDGDNGIQRHIYQCQKLLANLQNIEPRFAESLNMLASIEAE
                     LGEISANMRDVAGRSDINPNELAAQEQRMGELMGMARKYRIEPEELPAKLAEIEERLQ
                     SLQAAADLDALEHNVAHNFAEYQEAAHILSAMRHQAAERLSGETTEHMQHLAMKGARF
                     DIVLLPSSPTAHGLEQVQFQVAANKGNPPRPLNKVASGGELARISLALQVVASQYTQV
                     PTLIFDEVDTGIGGGVAEMVGKALRALGRKHQVLAVTHLPQVASCGENHWRVRKHSEG
                     EQTVSEISILDEIQRIEEVARMLGGEVITDTTRQHAAELLQLASKNSLF"
     gene            complement(1990..2592)
                     /locus_tag="ACFM8S_001082"
     CDS             complement(1990..2592)
                     /locus_tag="ACFM8S_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFC6314161.1"
                     /translation="MTDKISPDALIEAALLTQTEPLTEKSMRELCVPPLSQDKLIDVL
                     AQLKTRWQDRALQLVHTQEGWRFQIVQTAFERLGSLQEQRAPRYSRAVMETLAIIAYQ
                     QPVTRGDIEGIRGVAVSQNVMQTLQDRGWIEVIGHRDTLGKPALWATTATFLSDLRLD
                     GLEELPPLTELGELVLPDLMEMPPTDEEELEAVPSDTLPN"
     gene            complement(2693..3634)
                     /gene="ylqF"
                     /locus_tag="ACFM8S_001083"
     CDS             complement(2693..3634)
                     /gene="ylqF"
                     /locus_tag="ACFM8S_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFC6314162.1"
                     /translation="MAIQWFPGHMNKAKKAIADRAKSVDMVIEMLDARMPASSENPLL
                     AQLSKGKPKLKILNKQDLADPERTKVWLDHYNSRPDTRAIALDSSETGAHGKITQACR
                     AMIPHRQGIDKPLRVLICGIPNVGKSTLINGMIGKKSAKTGNEPGITKAEQRLFLADD
                     FWLYDTPGMLWPKIIVEESGYNLAAGGSVGRNALDEEEVALELLDYLRRHYLPMLQER
                     YRADKDPSSHWDDNAWLEWIAQKRGAVLSGGRVNYQKAAENILTDFREGKIGRITLET
                     PNQWETWLKKARQKEAELKAIREARKAERKGQKPSEA"
     gene            complement(3716..4570)
                     /gene="rapZ"
                     /locus_tag="ACFM8S_001084"
     CDS             complement(3716..4570)
                     /gene="rapZ"
                     /locus_tag="ACFM8S_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFC6314163.1"
                     /translation="MKIVLISGLSGSGKSVALRQMEDSGYFCVDNLPLEMLPALVSYH
                     IERADETELAVSVDVRSGIDIGQAREQIASLRGLGHRVEVLFVEAEESVLVRRFSETR
                     RGHPLSNQDMTLLESLKKEREWLFPLKEIAYCIDTSKMNAQQLRHAVRQWLKVERTGL
                     LVILESFGFKYGVPNNADFMFDMRSLPNPYYDPELRPYTGMDKPVWDYLDGQPLVQEM
                     VDDIEKFLTHWLPRLEDESRSYVTVAIGCTGGQHRSVYIVEKLARRLKGRYELLIRHR
                     QAQNLSDR"
     gene            complement(4551..5513)
                     /gene="hprK"
                     /locus_tag="ACFM8S_001085"
     CDS             complement(4551..5513)
                     /gene="hprK"
                     /locus_tag="ACFM8S_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFC6314164.1"
                     /translation="MPSISVRRLFDDNQYKLQLAWAAGNSGADNRIGVEADKPVLALV
                     GHLNFIHPNQIQVVGLAESEYLNRLESGETGYQFGDLFDISMSLVIVANGLPVSPGLR
                     DYCHKNDIPLLTSKLESPYLMDVLRIYLQRTLAASSVKHGVFLDVFEIGVLITGHSGL
                     GKSELALELISRGHSLIADDAVELFRIGPETLEGRCSPMLRDFLEVRGLGILNIRHIF
                     GETSIRPKKILQLIINLVEADDEYMKQLDRLSIRTETESILNVNVRSVTLPVAVGRNL
                     AVLVEAAVRNYILQLRGKDSTREFLERHQTQLKENEQHNENRPD"
     gene            complement(5517..5966)
                     /gene="ptsN"
                     /locus_tag="ACFM8S_001086"
     CDS             complement(5517..5966)
                     /gene="ptsN"
                     /locus_tag="ACFM8S_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFC6314165.1"
                     /translation="MSLIGEILPLSHIVLDMEVGSKKRLFEEAGLLLERESSLSHADV
                     FECLFAREKLGSTGLGQGVAIPHGRHAGVKQATGAFIRTREPVGFDAPDGKPVSLVFI
                     LLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVSSAEEARAILTEE"
     gene            complement(6150..7040)
                     /gene="ubiA"
                     /locus_tag="ACFM8S_001087"
     CDS             complement(6150..7040)
                     /gene="ubiA"
                     /locus_tag="ACFM8S_001087"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFC6314166.1"
                     /translation="MNPKSPLFLRLSDRLDVYLRLMRADKPIGTLLLLWPTYWALWLA
                     SDGIPNLAVLAAFTIGTFLMRSAGCVINDFADRDFDGAVERTKNRPFAQGRVKKKEAL
                     LLTAFLCLLAALCLIPLNHLTWLMSLPALFLALTYPFTKRFFPIPQLYLGLAFSFGIP
                     MAFAAVAGNVPPQAWILFAANVLWTLAYDTVYAMADKEDDLKIGIKTSAVTFGRYDIA
                     AVMLCHGGFTLLMAVLGAVIGAAWAYWTAIPIVLLLQYRQYAAIKSRLRQICFETFLA
                     NNRIGWVWFTAIFAHTFFAK"
     gene            complement(7070..7546)
                     /locus_tag="ACFM8S_001088"
     CDS             complement(7070..7546)
                     /locus_tag="ACFM8S_001088"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFC6314167.1"
                     /translation="MEHLFGKWLPDLPAAVSDGIDLPMSRLLKARSLTAALRALPHTF
                     SVELLKLGELETECGGRLVREVLLKLDGIPVVAARSECRIGSAFWQNILDCGTRPLGE
                     RLFQADLEGARSAFEFAVSGEGCGRHFAARRSRFSRHGEEMLLTEYFLPELKRFIG"
     gene            complement(7561..8208)
                     /gene="bioD"
                     /locus_tag="ACFM8S_001089"
     CDS             complement(7561..8208)
                     /gene="bioD"
                     /locus_tag="ACFM8S_001089"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFC6314168.1"
                     /translation="MKGVYFVSGIDTDIGKTVATGVLAKQLLQQGKSVITQKPVQTGC
                     QNIADDIAVHRKIMGKPMQEADKQGLTMPEIFSYPASPHLAARLDGRALDLEKIRTAT
                     QELAAQYEVVLVEGAGGLMVPLTENLLTIDYIQQQDYPVILVTSGRLGSINHTLLSFA
                     ALKQYGIRLHSLVFNHIHDSRDAHIAQYSLSYLKCRLKADFSEAEWMELAKTDAV"
     gene            complement(8205..9506)
                     /gene="bioA"
                     /locus_tag="ACFM8S_001090"
     CDS             complement(8205..9506)
                     /gene="bioA"
                     /locus_tag="ACFM8S_001090"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFC6314169.1"
                     /translation="MPSEHQHTSSLLDFDRTHLLHPYTSMTDPLPVYPVKRAEGVFIE
                     LADGTRLIDGMSSWWCAIHGYNHPVLNQAVETQMKQMSHVMFGGLTHEPAVELGKLLV
                     GILPQGLDRIFYADSGSVSVEVALKMAVQYQQARGLRAKQNMATVRRGYHGDTWNAMS
                     VCDPETGMHHIFGSTLPQRYFVDNPKSRFDDEWDGADLQPVRALFEAHHADIAAFILE
                     PVVQGAGGMYFYHPQYLRGLRDLCDEFDIVLIFDEIATGFGRTGKMFACEHAEVVPDI
                     MCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLL
                     LSQDWQANIRRIESILKGRLKAAWDIRGVKDVRVLGAIGVIELDKGVDMARFQADCVA
                     QGIWVRPFGRLVYLMPPYIISDGVLTKLADKTVQILKEHSK"
     gene            complement(9720..9959)
                     /locus_tag="ACFM8S_001091"
     CDS             complement(9720..9959)
                     /locus_tag="ACFM8S_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314170.1"
                     /translation="MNVWDIWGRMVEIYHKYKKPCLVLAVDFVMGMVFIEPNEELCIG
                     RCYAPMSESPDFANAVAMAVAMICIVWIAVLSMTD"
BASE COUNT         2325 a         2738 c         2514 g         2428 t
ORIGIN      
        1 ctatccaact gagctacggg tgcagcacaa tcacttgttc aagtgattaa gaaagcgtga
       61 taatacggat ttatctgagt tctgtctatt gttttagact tttattttta gttatttttt
      121 aactgattga ttttaaaata aactattttt cgacgcaagt tgcagcaatt ctgccgcatg
      181 ttgccgcgtc gtatcggtaa tgacttctcc gcccaacata cgggcaacct cttcgatccg
      241 ttggatttca tccaatatac tgatttcgct gacggtttgc tctccctcgc tgtgcttgcg
      301 cacccgccag tggttttctc cgcaggatgc gacttgggga aggtgggtaa cggcaagcac
      361 ctgatgtttt ctgcccaacg cacgtaatgc cttgccgacc atttcagcca ctccccctcc
      421 aataccggta tcgacctcat caaaaatcag ggtgggaact tgggtatatt ggctggcaac
      481 aacctgtaag gcaaggctga tacgcgccaa ttcgccgccg gaggcaactt tattcagcgg
      541 acggggcgga ttgcctttgt tggcggcaac ttgaaattga acctgctcca aaccgtgtgc
      601 cgtcggcgac gagggcaaca ggacgatgtc gaaacgtgca cctttcatgg caaggtgttg
      661 catatgctcg gtcgtttcgc cgctcaaacg ctctgccgcc tgatggcgca tggcagaaag
      721 gatgtgggca gcttcctgat attcggcaaa attgtgggca acattatgct cgagcgcgtc
      781 caaatcggcg gcagcttgca ggctttgcag gcgttcttcg atttctgcca acttggcagg
      841 caactcttca ggctcgatcc ggtatttccg cgccatcccc atcagttcgc ccatacgctg
      901 ttcctgtgcc gccaattcat tggggttgat gtcgctgcga cctgccacat cgcgcatatt
      961 ggcactgatt tcgcccaatt cggcctcgat gcttgccaac atattcaggc tctcggcaaa
     1021 gcgcggctcg atgttttgca gattggccaa tagtttttga cattgataga tatggcgttg
     1081 gatgccgttg tcgccgtcaa tcttgcttcc gacttcttcg gcagcctgca acagctcggc
     1141 agaatgggca aggctgtcgt ggctttggct gagggcttcc cattcgcctt gtttaatgtc
     1201 caactgattc aattcgttaa actgccattc cagacgctct tgttcgagtg caatgttttc
     1261 tgcctgttcc tgcgcttcct gcaatgcttt ttttgagttt gcccagtttt gataaagctg
     1321 cctgacggtt tccgcctgcg ccctgctgcc cgcaaatgcg tccaacaatt cgcgctgagc
     1381 ggcttcctga ttaagcgaat gatgggcgtt ttgcccgtgg atgtcgataa gctggctgcc
     1441 gacggctttg agttgcgcca aggtagcggc ctgattgttg ataaagctgc ggcttttgcc
     1501 tttggcatcg ataatgcggc ggatactgag ttcttcttct ccgtcgttta aaagcccctg
     1561 ttcatacaat tctgctttta aaacaggtaa atgggaaata tcaaacaacg ccgacaactg
     1621 cgcttctttt gcgccgctgc ggacttggct gtaatcggct ttatcgccca acagcagacc
     1681 aatcgcatcc aaagtaatgg acttgcccgc gccagtttct ccggtcaata cggtaaagcc
     1741 gctttgaaaa tccagattca gattttcgac aatgacaaaa tcacgcaaag aaagtgttag
     1801 aagcatcgtg aggattcctt agcaattcta ccgacaaaag caatatcaac tttagatata
     1861 cgaattataa ctaagatttt ttaaattaaa aataagtttt tccttaattt tgcttagtgc
     1921 ttgtttttat ctgcttattt gttgaaatgc cgtctgaagc aatgtgcgtt tcagacggca
     1981 tttggaattt cagttgggca gggtatcgga cggtacggct tccagctctt cttcatccgt
     2041 aggcggcatt tccattaaat cgggtaaaac cagttcgccc agttcggtca gcggcggaag
     2101 ttcttccaga ccgtccaagc gcaaatcgct gagaaaagtt gccgttgtcg cccacaatgc
     2161 gggttttccc aatgtgtccc gatgtccgat gacttcaatc cacccccgat cctgcaaggt
     2221 ctgcatcacg ttctgcgaca ccgccacgcc gcgtatacct tcgatgtcgc cgcgcgttac
     2281 gggctgctgg taggcgataa tcgccagtgt ttccatcacg gcgcgggagt aacgcggcgc
     2341 acgctgttct tgcaggctgc ccagccgctc gaatgccgtc tgaacaatct gaaaacgcca
     2401 gccctcttgc gtatgcacca gttgcaacgc cctatcctgc caacgcgttt tcaactgcgc
     2461 caacacatca atcagtttgt cctgcgacag tggcggcaca cacaattcgc gcatagattt
     2521 ttcggtcagc ggttcggttt gggtcagcag tgcggcttca attagtgcgt cgggagaaat
     2581 tttgtcggtc atacgggtat ccgtgctgaa acggcatggg tttggatatg ccgtctgaaa
     2641 tcggttggag tagagagaag ctgcctgaaa aatatttttc agacggcatt ctttatgctt
     2701 ccgaaggctt ctgcccttta cgctctgctt ttctggcttc gcgtatggct ttgagttcgg
     2761 cttctttctg acgggctttt ttgagccaag tttcccattg gttcggcgtt tcgagggtga
     2821 ttctgccgat tttgccttcg cggaaatcgg taaggatgtt ttcggcggct ttttggtagt
     2881 tgacccgtcc gccgctgagg actgcgccgc gtttttgggc tatccattcg agccaagcgt
     2941 tgtcgtccca gtggctgctc gggtctttgt cggcccggta gcgttcttgc aacataggga
     3001 ggtagtggcg gcggaggtag tctaaaagtt cgagggcgac ttcttcttcg tccaatgcgt
     3061 tgcgtccgac tgaaccgccg gcggcgaggt tgtagccgct ttcttcgaca atgattttcg
     3121 gccacagcat tccgggggtg tcgtacagcc agaagtcgtc ggcaaggaaa aggcgttgtt
     3181 cggctttggt gatgccgggt tcgttgccgg ttttggcgga ttttttgcct atcatgccgt
     3241 tgatgagggt ggatttgccg acgttgggaa tgccgcaaat caaaacgcgc aggggtttgt
     3301 ctatgccttg ccggtgggga atcatcgcgc ggcaggcttg ggtgattttg ccgtgtgcgc
     3361 cggtttcgga ggaatcgagg gcgatggcgc gcgtgtcggg gcggctgttg tagtggtcaa
     3421 gccagacctt tgtgcgttcg gggtcggcaa gatcttgttt gttgaggatt ttaagtttag
     3481 gtttgccttt ggaaagctgg gcaagcaggg ggttttcgct ggaggcgggc atacgcgcgt
     3541 ccagcatttc aatcaccata tcaacgcttt ttgcacgatc ggcgatggct tttttcgcct
     3601 tgttcatatg gccgggaaac cattggattg ccatgtctgt tctttcttct caatatttta
     3661 aatgccgtct gaaacaaaaa agcggggttt cagacggcat aatggtttga cggaattagc
     3721 ggtctgacag gttttgcgcc tgtctgtgcc gtatcagcaa ttcataacgc cctttcaacc
     3781 ttcgggcgag tttttcgaca atatagaccg aacggtgctg tcctcccgtg caaccgatgg
     3841 cgacggtaac gtagctcctg ctttcatcct ccaaacgcgg cagccaatgc gtcagaaatt
     3901 tttcgatgtc gtcaaccatt tcctgcacaa gcggctgtcc gtccaaataa tcccaaacgg
     3961 gcttgtccat accggtgtaa ggcctcaact cgggatcgta atacgggttg ggcagactgc
     4021 gcatatcgaa cataaaatcc gcgttgttcg gcacgccgta tttgaacccg aaggactcca
     4081 aaatcaccag cagccctgta cgttcgacct tcagccactg ccggactgca tggcggagct
     4141 gttgggcatt catcttggaa gtgtcgatac aataggcgat ttctttaagc gggaacagcc
     4201 attcccgttc tttctttaag ctttccaaca gggtcatatc ctgattgctc agaggatgtc
     4261 ctcgcctggt ttcggaaaac cggcggacca acacgctttc ttccgcctcg acaaacaaaa
     4321 cttcaaccct gtgccccagt ccgcgcagag aggcaatctg ttcccgcgcc tgtccgatgt
     4381 ctatgccgga acgcacatcg acgctgaccg ccaattcggt ttcgtccgca cgctcgatat
     4441 gatacgacac cagcgcgggc aacatttcca aaggcaaatt gtccacgcag aaataacccg
     4501 aatcttccat ttggcgcagt gcgacggatt tgcccgaacc ggacaacccg ctaatcagga
     4561 cgattttcat tgtgttgctc gttttcttta agctgcgtct gatgacgttc caaaaattcg
     4621 cgcgtactat ccttaccgcg caactgaaga atataattgc gtaccgccgc ctcaaccaaa
     4681 acggcgaggt tgcgtccgac ggcgacgggc agcgtaaccg aacggacgtt gacgttgagg
     4741 atggattcgg tttcggtgcg gatgctcaac cggtcaagct gcttcatata ctcgtcgtcc
     4801 gcctcgacca aattgataat gagttgcagg atttttttgg ggcggatgga agtttcgccg
     4861 aaaatatggc ggatattgag tatccccaag ccgcgtactt ccaaaaaatc gcgcagcata
     4921 ggcgaacaac gtccttccaa tgtttccggg ccgatgcgga acagctcgac cgcatcgtcg
     4981 gcaatcaggc tgtggccgcg cgaaatcagt tccaatgcca attcgctctt acccaaaccc
     5041 gaatgtccgg taatcagcac gccgatttca aacacatcga gaaatacgcc gtgtttgacg
     5101 gacgatgccg ccagggtgcg ttgcaggtaa atccgcaaca catccatcag ataggggctt
     5161 tcgagtttgg aagtcagcag tggaatatcg tttttatggc agtagtcgcg cagtcccggg
     5221 gaaaccggca agccgtttgc cacaatgacc aaagacatag aaatgtcgaa caggtcgccg
     5281 aactgataac ccgtttcccc cgattcgaga cggttcagat attccgactc cgccaaaccg
     5341 accacttgga tttggttggg gtggataaaa ttcaggtgtc cgaccagggc gaggacgggc
     5401 ttgtccgcct ctacgccgat acggttgtcc gcacccgaat tgccggcggc ccaggcaagt
     5461 tgcaacttgt attggttgtc atcaaacagt cggcggacgg agatactggg catattttat
     5521 tcttcagtca ggatggcgcg ggcttcttcc gcagaagaaa ccgtcatcag cgattctctg
     5581 atgctttttt gggaaaactt gccggccagt ttggacaata cttccaaatg ctcacccgtc
     5641 gcattttccg gaaccagcaa gataaaaacc agggaaaccg gtttgccgtc cggtgcgtca
     5701 aatccgacgg gttcgcgcgt gcggatgaac gcgcccgtcg cctgcttcac gccggcatga
     5761 cggccgtgcg ggatggcaac gccctgcccc aaaccggtcg aaccgagttt ttcacgggca
     5821 aaaagacatt cgaaaacatc agcatgggac aatgaggatt cgcgttccaa aagcaggccc
     5881 gcttcctcaa acagcctttt tttactgccc acctccatat ccaaaacaat atgggacaaa
     5941 ggcaaaattt cgccgataag gctcataagc ttctctttca gacatcgcaa aacaggaaga
     6001 ttgtaccgac tgccggggca aatctcaatc ccgcatacgg tacgggctga cataacacag
     6061 cgttttaaaa acatatttta acgctcttcg gcacagatag aaatgccgtc caaagcggtt
     6121 tacggctctt cagacggcat tgccctgcct tatttcgcga aaaacgtatg ggcaaaaatg
     6181 gcggtaaacc acacccaacc aattctgttg tttgccaaaa acgtttcaaa acagatttgc
     6241 cggaggcggc ttttgatggc ggcatattgg cggtattgca gcagcaggac gatggggatt
     6301 gccgtccaat atgcccatgc cgcaccgata accgcaccca atactgccat cagcagggta
     6361 aagccgccat gacacagcat aacggcggcg atgtcgtaac gcccgaacgt gacggcggag
     6421 gttttgatgc cgattttcaa atcgtcttct ttgtccgcca ttgcataaac cgtgtcatac
     6481 gccagagtcc ataacacatt ggcggcaaag agtatccacg cttgaggcgg cacgtttccg
     6541 gcaacggcgg caaacgccat cgggataccg aaggaaaagg caagcccgag atagagttgg
     6601 ggaatcggaa aaaaacgttt ggtaaacggg taagtcagcg caagaaacag cgcgggcagg
     6661 ctcatcagcc atgtcagatg attcagcgga atcaggcaca atgcggcaag caggcacaaa
     6721 aatgccgtca gcagcagcgc ttcttttttc ttgacccggc cctgtgcgaa cggacggttt
     6781 ttagtgcgct cgacagcccc gtcaaaatcg cggtcggcaa aatcgttgat gacgcaaccg
     6841 gcactgcgca ttaaaaacgt accgattgta aacgccgcca ataccgccaa attgggaatg
     6901 ccgtctgaag ccagccacaa tgcccagtag gtcggccaca gcaaaagcag cgtcccaatg
     6961 ggcttgtccg cccgcatcag gcgcaggtac acatccaaac gatcggacag tcgtaaaaat
     7021 aaaggggatt taggattcat attgccgcac aacttgaaaa aacggtattt tatccgataa
     7081 agcgtttcag ttcgggcaga aaatactcgg tcagcagcat ttcctcgccg tgacgggaaa
     7141 accgagaacg ccgcgcggca aagtgccgtc cgcatccttc gccggaaacg gcaaactcaa
     7201 acgccgaacg cgccccttcc aaatcggctt gaaacagacg ctcgcccaaa gggcgcgtgc
     7261 cgcagtccaa aatgttttgc caaaacgccg aacctatccg gcactcgctc cttgccgcga
     7321 caacagggat accgtccagc ttcaacaaaa cttcgcgcac cagcctccct ccgcattccg
     7381 tctccaattc gcccagtttc agcagttcca ccgaaaatgt atgcggcaag gcgcgcaatg
     7441 cggcggtcag cgaccgggct ttcaataacc ggctcatcgg caggtcgatg ccgtctgaaa
     7501 cggcggcggg caagtcgggc agccatttcc caaataggtg ttccatattt ttcccaatct
     7561 ttataccgcg tctgtttttg ccaactccat ccattccgct tcggaaaaat cggctttcag
     7621 acggcatttc aagtagctca ggctgtattg ggcgatgtgt gcgtcgcggc tgtcatgaat
     7681 atggttgaat acgaggctgt gcaagcgaat gccgtattgt ttgagcgcgg cgaaactgag
     7741 taaagtgtgg ttgatactgc ccagccgtcc gctggtaacg aggatgacgg gataatcttg
     7801 ttgctgaata taatcaatgg ttaacagatt ttccgtcagc ggaaccatca acccgcccgc
     7861 gccttcgacc aaaacgactt cgtactgcgc cgccaattct tgtgtggcgg tgcggatttt
     7921 ctccaagtcc aaagccctgc cgtcaagacg ggcggcaagg tggggcgaag cgggatagct
     7981 gaagatttca ggcatggtca gcccttgttt gtcggcttcc tgcatgggtt tacccatgat
     8041 tttacgatgg acggctatgt cgtcggcaat gttttggcaa ccggtttgca cgggcttttg
     8101 cgtaatcacg cttttgccct gctgcaacaa ttgttttgcc aacacgccgg tggcgacggt
     8161 tttgccgatg tccgtgtcta tgccgctgac gaagtaaacg cctttcattt gctgtgttcc
     8221 ttcaagattt gcacggtttt gtcggcaagt ttggtcaaaa cgccgtctga aataatatac
     8281 ggcggcatca gatacaccag cctgccgaac gggcgcaccc aaatgccctg agccacgcaa
     8341 tctgcttgaa aacgcgccat atccacgcct ttatccagct cgatcacgcc gatggcaccc
     8401 aaaacgcgta cgtctttcac gccgcgaata tcccacgcgg ctttcagacg gccttttaag
     8461 atgctttcaa tgcggcggat atttgcctgc cagtcttgag acaaaagcag tttgaccgaa
     8521 gcgcaggcaa cggcacacgc cagcgggttt gccataaacg tcgggccgtg cataaacacg
     8581 cccgcttcgc cgcgcgaaat cgtttcggta actttttgcg aagtgattgc tgccgccagc
     8641 gtcatatagc cgccgctcaa acccttgccg atgcacataa tatccggcac gacctccgcg
     8701 tgttcgcagg caaacatctt gcccgtgcgc ccgaatccgg tggcgatttc atcaaaaatc
     8761 aaaacgatat caaactcgtc gcacaaatcg cgcaatccgc gaagatactg cggatgataa
     8821 aaatacatgc cgcctgcgcc ctgcacgacc ggttccaaaa taaaggcggc aatatccgca
     8881 tgatgcgctt caaacaaggc gcggacaggc tgcaaatccg ccccgtccca ttcatcatcg
     8941 aaacggcttt tcggattatc gacaaaataa cgctgcggca gagtgctgcc gaaaatatgg
     9001 tgcatccccg tttccggatc gcagacggac atcgcgttcc aagtatcgcc gtgatacccg
     9061 cgccgcaccg tcgccatatt ttgttttgca cgcaaacccc gcgcctgctg gtattgcacc
     9121 gccatcttca acgcgacttc caccgaaacc gaacccgaat ccgcataaaa aatacgatcc
     9181 agcccctgcg gcaaaatccc gaccaacaat ttgcccagct ccaccgccgg ctcatgcgtc
     9241 aaaccgccga acatcacatg cgacatctgt ttcatctgcg tctcaaccgc ctgattcaaa
     9301 acgggatgat tgtaaccgtg tatcgcacac caccaagacg acatcccgtc aatcagccgc
     9361 gtgccgtccg ccaattcgat aaacacccct tctgcacgtt tgacaggata aacgggaagc
     9421 ggatcggtca tggaagtata gggatggagc agatgggtac ggtcgaaatc aagcaatgat
     9481 gatgtgtgtt gatgttcaga cggcataagt ttctctcttt tcttattgct gcattcaaac
     9541 gcaaaacgcg tattctactc cgacagaccg cttcccacac ctttccatcc gtttcgggcg
     9601 taaaaccacg caacaaatcg tccgcagtat aagcgcacac cgtttcgcat tccccaagcc
     9661 cgattggaat cagacgaccc aacgcccaat attgtttcac acagcctccg cccaacgctt
     9721 caatcagtca tagacaaaac ggcaatccat acgatacaga tcatagcaac agccatcgca
     9781 acagcgttag caaaatcagg ggactccgac ataggcgcat agcatctacc gatgcacagc
     9841 tcctcattcg gctctatgaa taccataccc atcacaaaat ccaccgccaa aaccaggcac
     9901 ggcttcttat acttatgata gatttccacc atcctgcccc atatatccca aacattcata
     9961 ccgcatatcc cgcaggcaac aaattccgat tgaaggttac agccc
//
LOCUS       DAXHSF010000061         9794 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.061, whole genome shotgun sequence.
ACCESSION   DAXHSF010000061 DAXHSF010000000
VERSION     DAXHSF010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9794
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.061"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(10..1305)
                     /locus_tag="ACFM8S_001092"
     CDS             complement(10..1305)
                     /locus_tag="ACFM8S_001092"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFC6314171.1"
                     /translation="MIRPDFQEYLPSYYFSSVNPHTVYPKLQCRLKTDTCIIGGGLSG
                     LCTALPLAEQGHETVVLEAARIGFGASGRSGGQVISDYACGMEEIEKQVGLEQAQWFW
                     QQSLQAIELVDERVRKHTIDCDWQRGYATVAVRPQHWEELQQWHEHAQRHYGASHYQL
                     WDNTELKQQLDSDMYQGAQFDPLSGHLHPLNYTLGIARAAAEAGTKIFEHSPMIRIEP
                     YQNGWLVYTPEGSVECKNLVYAVNTYAGLNPIFRPLERKAIAVSTFIIATEPLGARAK
                     GLIRNNMAVCDNRHILDYYRLSADGRLLFGGKDNEFIDNPARMTELVRQDMLKVFPQL
                     ADVKIEYSWGGECDITANLVPHFGRLAPNVFYTQGYSGHGMAITGIAGLAVAEAILGD
                     ECRLKPFERLRQPNIILQPFLRKLGSFLGSKYYQWKDSR"
     gene            complement(1431..2318)
                     /locus_tag="ACFM8S_001093"
     CDS             complement(1431..2318)
                     /locus_tag="ACFM8S_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFC6314172.1"
                     /translation="MQKSKLSWFLKLMLALSLAFLYIPLVVLVIYSFNESKLVTVWGG
                     FSTKWYGALLENDTILEAAWLSLRIAVVSSLAAVVLGTLAGYAMARIKRFRGSTLFAG
                     MISAPMVMPDVITGLSMLLLIIQVQIFLQGSEWLQHLYFDRGFFTIFLGHTTLCMAYI
                     TVVIRSRLVELDQSLEEAAMDLGARPLKIFFVITLPLIAPAIASGFLLGITLSLDDLV
                     ITSFLSGPGSSTLPQVIFSKIKLGLDPQMNVLATILIGIIGTLVIIVNYWMMRQATKR
                     DREAAEAYRQEKLAAEKAN"
     gene            complement(2318..3283)
                     /locus_tag="ACFM8S_001094"
     CDS             complement(2318..3283)
                     /locus_tag="ACFM8S_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFC6314173.1"
                     /translation="MNLNKLKNKLFRRPGQRVVIAVPYIWLLVLFLIPFAIVLKISFA
                     EQEIAIPPFTPLTSIDEDLGRLNITISYQNYADIFQNFWHTLNPFGDSENSNIYLMTY
                     WSSIKTALMTTVICLLVGYPTAYAISRANPSVRNGLLLAIMLPFWTSFLLRVYAWMGL
                     LGHNGIVNNLLIKMGIISEPLDLFYNAFSLNLVMVYAYLPFMILPLYTQLVKLDNRLL
                     EAASDLGAGPVKSFLTITLPLSKTGIIAGSMLVFVPAVGEFVIPELVGGSENLMIGKV
                     LWQAFFDQNNWPLASAVAVVMVALLVVPIALFQHYENRELEEGAK"
     gene            complement(3299..4423)
                     /gene="potA"
                     /locus_tag="ACFM8S_001095"
     CDS             complement(3299..4423)
                     /gene="potA"
                     /locus_tag="ACFM8S_001095"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HFC6314174.1"
                     /translation="MTATTASSAKPYLKIQGLVKKFGDNYAVDNIDLDIYQHEIFALL
                     GSSGSGKSTLLRMLAGMESPNQGKIILDGQDITKLAPYDRPINMMFQSYALFPHMTVE
                     QNIAFGLKQDKMPKGEIAARVEEMLRLVQMTKFAKRKPHQLSGGQQQRIALARSLAKR
                     PKILLLDEPLGALDKKLRQQTQLELVNTLEQVGVTCIMVTHDQEEAMTMATRIAIMSD
                     GQLQQVGTPSDVYDYPNSRFTAEFIGETNIFDGVVIEDHADYAVIECEGLENHVRIDH
                     GLGGPSEQDLWVSIRPEDIDLYKEKPEYLGDYNWAKGTVKEIAYLGSFAIYHIKLANG
                     RVVKSQVPAPYWYVRNITPPTWDETVYISWPENQPTPLFR"
     gene            complement(4618..4725)
                     /locus_tag="ACFM8S_001096"
                     /pseudo
     CDS             complement(4618..4725)
                     /locus_tag="ACFM8S_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4804..5073)
                     /gene="rpsO"
                     /locus_tag="ACFM8S_001097"
     CDS             complement(4804..5073)
                     /gene="rpsO"
                     /locus_tag="ACFM8S_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFC6314175.1"
                     /translation="MALTVEQKAQIVKDFQRKEGDTGSSEVQVALLTFRINDLTPHFK
                     ANPKDHHSRRGLLKMVSQRRRLLAYLRRTQPDTYRALITRLGLRK"
     gene            complement(5296..6231)
                     /gene="secF"
                     /locus_tag="ACFM8S_001098"
     CDS             complement(5296..6231)
                     /gene="secF"
                     /locus_tag="ACFM8S_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFC6314176.1"
                     /translation="MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVTRGLNFSVE
                     FTGGTVMEVQYQQGADVNKMRERLDTLKIGDVQVQALGTNKHIMIRLPNKEGVTSAQL
                     SNQVMDLLKKDSPDVTLRQVEFIGPQVGEELVSNGLMALGFVVIGIIIYLSMRFEWRF
                     AVSAIIANMHDIVIILGCFAFFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRK
                     PAMRGHAVPEVIDNAITATMSRTIITHGSTEAMVVSMLVFGGAALHGFSMALTIGIVF
                     GIYSSVLVASPLLLMFGLSRDNIGKEPKKKEEIVV"
     gene            complement(6235..8088)
                     /gene="secD"
                     /locus_tag="ACFM8S_001099"
     CDS             complement(6235..8088)
                     /gene="secD"
                     /locus_tag="ACFM8S_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFC6314177.1"
                     /translation="MNRYPLWKYLLIVFTIAVAAVYSLPNLFGETPAVQVSTNRQAII
                     INEQTQFKVDAALKNAGIQTDGMFVVDNSLKVRFKDTETQLKARDVIENTLGEGYITA
                     LNLLADSPEWMEKIKANPMFLGLDLRGGVHFTMQVDMKAAMQKTFERYSGDIRRELRR
                     EKIRSGTVRQAGNSLTVPLQDAGDVQKALPQLRKLFPEATLNSDGSNIVLTLSEEAVN
                     KVRSDAVKQNITTLHNRVNELGVAEPVIQQSGADRIVVQLPGVQDTAKAKDIIGRTAT
                     LELRMVEDDPAKLREALEGNVPSGYELLSSGGDRPETLLISKQVELTGDNINDAQPSF
                     DQMGAPAVSLSLDSAGGSIFGELTAANVGKRMAMVLIDQGKSEVVTAPVIRTAITGGR
                     VEISGSMTTAEANDTSLLLRAGSLAAPMQIVEERTIGPSLGKENIEKGFHSTLWGFAI
                     VAAFMVVYYRLMGFFSTIALSANILFLIGILSAMQATLTLPGMAALALTLGMAIDSNV
                     LINERIREELRAGVPPQQAINLGFQHAWATIVDSNLTSLIAGIALLVFGSGPVRGFAV
                     VHCLGILTSMYSSVVVFRALVNLWYGRRRKLQNISIGAVWKPEAETAAGKE"
     gene            complement(8300..8566)
                     /gene="yajC"
                     /locus_tag="ACFM8S_001100"
     CDS             complement(8300..8566)
                     /gene="yajC"
                     /locus_tag="ACFM8S_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFC6314178.1"
                     /translation="MNQAVAQFAPLVLIMVVFYFLIMRPQQKKFKAHQAMLAALKVGD
                     KVVLAAGFKGKVTRVGEQFFTVDIGQGTKIEVEVERNAIAAKVD"
     gene            8694..>9794
                     /locus_tag="ACFM8S_001101"
     CDS             8694..>9794
                     /locus_tag="ACFM8S_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HFC6314179.1"
                     /translation="MSLTRLILKFYALLRLFLGKNARTAWISHPACAGHEPGANHPDS
                     PDRILCIEQALRRAGIWQHLQTIEAEEISDTRLALVHSSKYLNRLESCLPQKGKIFRL
                     DNDTAISTGSLSAAHFAAGSAVQAVDMVMNRKAWHAFCAARPPGHHAGSGKAGGFCLL
                     NNVAAGVMHAIAEYRLKRIAVIDFDVHYGDGTAEIFKDDPRILFFNLFETDLFPFPEN
                     NDMPDGGNMVHLPLPPGTGSRTFREAVRRQWLPRLAAFKPELVLLSAGFDAHRLDESG
                     RLNLHEADFAWLTHKIIQTASGCPGKIVSVLEGGYTLEPLAQSAAEHIRVLAGLGKSD
                     AATAYQKTLDPTKKRFAKPKTGQVRQPTQSDRY"
BASE COUNT         2508 a         2574 c         2433 g         2279 t
ORIGIN      
        1 ctgcgacgct taacggctgt ctttccactg ataatatttc gagccgagga aagaaccgag
       61 tttgcgcaaa aacggttgca ggataatatt cggctggcgc aaccgctcaa acggcttcag
      121 acggcattcg tcccctaaaa ttgcttcggc aaccgccaga cctgcaatgc ctgttatcgc
      181 catcccgtgt ccggaataac cttgcgtata aaaaacattc ggggctaaac gtccgaaatg
      241 cgggacaagg ttggcggtaa tgtcgcactc cccgccccac gaatattcga ttttgacatc
      301 ggcaagctgc ggaaaaactt taagcatatc ttggcggaca agctcggtca tacgcgcggg
      361 attgtcgata aactcgttat ccttaccgcc gaaaagcagt ctgccgtccg cgctgaggcg
      421 gtaataatcc aaaatatggc ggttgtcgca tactgccata ttgttgcgga taagcccttt
      481 tgcacgcgcg cccaagggtt ctgtggcaat aataaaggtg ctgacagcaa tcgccttgcg
      541 ttccaaaggc cggaatatcg gattcaaacc ggcataagta ttgacggcgt acaccagatt
      601 tttgcattcg acgctgcctt cgggggtgta aaccagccaa ccgttttgat aaggttcaat
      661 gcgtatcatg ggagaatgct caaaaatctt cgtaccagct tcggcagcgg cgcgggcgat
      721 gcccaacgtg taattgagcg gatggagatg cccggacaag ggatcgaact gtgcgccttg
      781 gtacatatcg ctgtcaagct gctgcttcag ttcagtgtta tcccagagtt gataatgact
      841 cgcaccgtaa tgccgttggg cgtgttcatg ccattgttgc aattcttccc aatgctgcgg
      901 acggacggca accgtggcat aaccgcgctg ccaatcgcaa tcgatggtat gtttgcggac
      961 gcgttcgtcc accagttcga tcgcctgcaa agactgttgc caaaaccatt gcgcctgttc
     1021 caaaccgacc tgtttttcaa tttcctccat accgcaggcg taatcgctga taacctgccc
     1081 gccactccgt cccgacgcgc cgaaaccgat acgcgcggct tccaacacaa ccgtttcatg
     1141 tccctgctcc gccaagggca atgcagtgca caaaccactc aatccgccgc cgatgataca
     1201 ggtatcggtt ttcagacggc attgaagttt cggataaaca gtatgaggat taaccgaact
     1261 gaaataataa gaaggcagat attcttgaaa atcagggcga atcattgtgt ttgctttatc
     1321 gggtatattt tcggacggaa tgatacaggc tgtcgagcca tatcgtccaa acagaaaatc
     1381 ggttgaagaa aacaggctga cccagtcatg cggtcagcct gccttattaa ttaatttgct
     1441 ttctcggcag ccaatttttc ttggcggtag gcttctgccg cttctcggtc acgcttggtt
     1501 gcctgcctca tcatccaata attgacgatg atgaccaatg ttccgatgat gccgattagg
     1561 atggttgcca aaacgttcat ctgcggatcg agacccaact tgattttgga gaaaatcacc
     1621 tgcggcaatg tggatgaacc ggggccggag aggaatgagg taattaccaa atcatccaaa
     1681 gacagggtaa tgccgagcag aaagcctgaa gcgatggcag gggcaatcaa aggcaaagtg
     1741 atgacaaaaa agattttcag cggacgcgcg cccaaatcca ttgcggcttc ttcgagcgat
     1801 tggtcaagct caaccagacg cgaacggata acaacggtaa tgtacgccat acacagcgtc
     1861 gtatgtccga ggaagatggt gaaaaagcca cgatcgaagt agagatgttg taaccattcg
     1921 ctgccctgca aaaatatctg tacctgaata atcagcagca gcatagacag accggtaatc
     1981 acgtcgggca tcaccatagg tgcggaaatc ataccggcga acaaggtact gccgcgaaaa
     2041 cgtttaatcc gcgccatcgc atagcctgcc agcgtgccca aaacgacggc agcaagcgaa
     2101 gacacaacgg caatccgcag cgacagccaa gcggcttcca agatggtgtc gttttccagc
     2161 aatgcgccgt accacttggt cgaaaagccg ccccaaacgg ttaccagctt ggattcgtta
     2221 aacgaataga tgaccaaaac aaccagcggg atatacagaa acgccagcga cagtgccaac
     2281 atcagtttca agaaccaaga taatttggat ttctgcatta tttggctcct tcttccaatt
     2341 cgcggttttc ataatgctga aacagggcaa tcggcacgac cagcagcgcg accatcacga
     2401 cggcgacggc ggaagccagc ggccagttgt tttgatcgaa gaacgcctgc cacaagactt
     2461 taccaatcat caggttttcc gaaccgccga ccagctcggg aatgacgaac tcgccgacag
     2521 cagggacgaa aaccagcatg gagcctgcaa taatgccggt tttcgacaaa ggcagggtaa
     2581 tcgtcaagaa cgatttgacc ggccccgcgc ccaaatcgga agccgcttca agcaggcggt
     2641 tgtcgagttt caccagttgc gtgtatagcg gcagaatcat aaacggcaga taggcgtaaa
     2701 ccatcaccaa attgagcgaa aaggcattgt agaacaaatc caaaggctcg ctgataatac
     2761 ccattttaat caacaggttg tttacaatac cgttatgccc gagcagaccc atccacgcat
     2821 agacgcgcaa caggaacgat gtccaaaagg gcagcataat ggcaagcagc aaaccattgc
     2881 ggacagaagg attggcacga gaaatcgcat aggcggtcgg ataaccgacc aacaggcaaa
     2941 tcactgtcgt catcaatgcg gttttgattg aagaccagta ggtcatcaga tagatattgc
     3001 tgttttcact gtcgccgaac gggttaaggg tatgccaaaa attttggaaa atatcggcat
     3061 agttttgata actgatggtg atattcagac gacctaaatc ttcgtcgatg ctggtcagcg
     3121 gcgtaaacgg cggaatggcg atttcttgtt cggcaaagct gattttcagc acgatggcga
     3181 acggaatcag aaacagcacc aaaagccaaa tatacggtac ggcaatcacc acacgctgcc
     3241 ccgggcggcg gaacagtttg tttttcagtt tattaaggtt cattgcattc cccttaaatc
     3301 aacggaacag tggcgtaggc tggttttccg gccagctgat atacacggtt tcgtcccaag
     3361 ttggcggtgt aatgttgcgt acataccagt aaggtgcagg aacttggctt ttgacgacgc
     3421 gcccgttggc gagtttgata tggtatatgg caaagctgcc caaataggcg atttctttta
     3481 ccgtgccttt cgcccagttg tagtcgccca aatattcggg tttttcttta tataaatcaa
     3541 tatcctctgg tcgaatacta acccaaaggt cctgctcgct cggaccaccc aaaccgtgat
     3601 cgatgcggac gtggttttcc aaaccttcgc attcgataac ggcatagtcg gcatgatctt
     3661 caatcaccac accgtcaaag atgttggttt cgccgataaa ctcggcagtg aagcggctgt
     3721 tgggatagtc gtacacgtcg ctgggtgtgc cgacttgctg caactgaccg tcagacataa
     3781 tggcgatgcg ggtcgccatc gtcatcgcct cttcttggtc gtgcgtaacc ataatacagg
     3841 ttacgccgac ttgttccagc gtattgacca actcaagctg ggtttgttgg cgcagttttt
     3901 tgtccaatgc accgaggggc tcatccagca gtagaatttt cggacgtttt gccagactgc
     3961 gtgccaaagc aatgcgctgc tgctgaccgc cggacaattg gtgcggtttg cgtttagcaa
     4021 atttggtcat ctgaaccagg cggagcattt cttcgacgcg cgcggcgatt tcgcctttag
     4081 gcattttgtc ctgtttcaga ccgaaggcaa tgttttgttc tacggtcata tgcggaaaaa
     4141 gcgcgtaact ttggaacatc atattgatgg ggcgatcata gggtgcaagt ttggtaatat
     4201 cctgaccatc aaggataatt tttccctgat tgggactttc catacccgcc agcatacgca
     4261 gcagtgtaga ttttccgctg ccggaactgc ccaaaagggc gaagatttcg tgttgataaa
     4321 tgtccaagtc gatgttatcg acagcgtaat tgtcaccaaa ctttttcacc aaaccttgga
     4381 ttttgagata aggtttggct gaagacgcag tggttgcggt cataatggca atactccaat
     4441 aaaaagacga gtaccggcaa aacggatttt cgaatgggtg ataaaaagct gtttgattgc
     4501 tggcgggagt taaacgtttg atgccgtctg aaactcttgt aaagcgcacg ggcagcatga
     4561 aatggaacaa gattccaaag aactttatat tatattagtt tatgcggttt tcgggcaata
     4621 tagtggatta aatttaaacc agtacagcgt tgccttgcct tgccgtacta tttgtactgt
     4681 ctgcggcttc gtcgccttgt cctgatttaa atttaatcca ctataaatat ggatttgagc
     4741 ttgtcggaaa gcaacagaaa agaaaacacc gcccattttt ctgggcggtg tcggaaagcg
     4801 taattattta cgcagaccca agcgggtaat caacgcgcga tacgtatcgg gctgggtacg
     4861 gcgcaagtag gccagcaggc ggcggcgttg gctgaccatt ttcaacaggc cgcgacggct
     4921 gtggtggtct ttggggttgg ctttgaagtg gggggtcagg tcgttgatgc ggaaagtcaa
     4981 cagagcgact tgtacttcgg aagagccggt gtcgccttct ttgcgttgga aatctttaac
     5041 gatttgtgct ttttgttcta cggtcagtgc cataatgaaa actccaaaaa tataagaatc
     5101 cccataggga ttcagacaag tttgccaagc ctgaagacaa cggcaaactc cctatgccca
     5161 agataggaca acgtggcatt atgacacaat tcccgcattt ctgcacaata ttttaagacc
     5221 gtggaagtaa ccgttaaaaa tgccgtctga agcattgtct gcttgagacg gcaatgttca
     5281 gacggcatat gcgcttcaaa ccacgatttc ttctttcttc ttcggttctt taccgatatt
     5341 gtcgcggctc aaaccgaaca ttagcagaag cgggctggca accaatacgg aagaataaat
     5401 gccgaacacg atgccaatgg tcaacgccat agaaaagccg tgcaaggccg caccgccgaa
     5461 caccagcatg gatacgacca tcgcctcggt cgaaccgtgg gtaatgatgg tgcggctcat
     5521 cgttgcggta atcgcgttgt cgatgacttc cggcacagca tgtccgcgca tcgccggctt
     5581 gcggaagttt tcacggatac ggtcgaagac gacgacggat tcgttcacag aatagcccaa
     5641 tacggcaagg atacccgcca agacggtcag cgaaaattcc cattggaaga aggcaaagca
     5701 gccgagaata atcacgatgt cgtgcatatt ggcgataatg gcagatacgg caaaacgcca
     5761 ttcaaaacgc atcgacaggt aaataatgat gccgataacg acaaaaccta aagccatcaa
     5821 tccattactt accaattcct caccgacttg cgggccgata aattcgactt ggcgcaaggt
     5881 aacgtcggga ctgtcttttt tcagcaaatc cataacctga ttggacaact gtgcggaagt
     5941 aacaccttct ttgttcggca ggcggatcat gatgtgtttg ttcgtaccca atgcctgaac
     6001 ctgtacatca cctattttca gcgtatcgag gcgttcgcgc atcttattga catccgcacc
     6061 ctgctgatat tggacttcca ttaccgtacc gccggtaaat tcgacagaga aattcagacc
     6121 tctggtaacc aaaaagaata cggcggcaat aaaggtaacc aacgaaataa aggtcgtcag
     6181 tttgccgtag ctcataaacg gaatatcgcg tttgatttta aagagttcca tagcttactc
     6241 cttacctgcc gcagtttcgg cttcaggttt ccataccgca ccgatggaaa tattctgcaa
     6301 tttgcgtctg cgtccgtacc acagattgac caacgcacgg aatacgacga cggaggaata
     6361 catcgaagtc agaataccca aacagtgtac gaccgcaaaa ccgcgtaccg ggccggaacc
     6421 gaataccaaa agcgcgatac cggcaatcag cgaagtcagg ttggaatcga caatggtcgc
     6481 ccatgcgtgt tggaaaccga gattgattgc ctgctgcggc ggcacgccgg cacgcaattc
     6541 ttcgcggata cgttcgttaa tcaagacgtt ggagtcgatt gccataccca aagtcaacgc
     6601 cagcgcggcc atacccggta acgtcaacgt tgcctgcatg gcagacaaaa taccgattag
     6661 gaacagtatg ttggcactca atgcaatggt agaaaagaaa cccatcagac gatagtaaac
     6721 caccatgaat gcagcaacga tggcaaaacc ccataaagtc gaatggaagc ctttttcgat
     6781 gttctcctta cccaaagacg gaccgatggt acgttcttcg acaatctgca tcggcgcggc
     6841 aagagaaccg gcacgcaaca gcaaagacgt atcattggct tcggctgtcg tcatgcttcc
     6901 ggaaatttcc acgcgtccgc ctgtaatggc agtacggata accggcgcgg ttacaacctc
     6961 ggattttcct tggtcgatca aaaccatcgc catgcgtttg ccgacatttg cggcagtcag
     7021 ttcgccgaaa atgctgccgc ccgcgctgtc caagctcaga ctgacggcag gtgcgcccat
     7081 ttggtcgaaa ctcggttgcg catcgttgat gttgtcgccc gtcagctcga cctgtttgct
     7141 gattagcaga gtttcgggac gatctccgcc gcttgaaagc agctcataac cgctcggcac
     7201 gttgccttcc aatgcctcgc gcaacttggc aggatcgtcc tccaccatac gcaattccaa
     7261 agtcgcggta cggccgatga tgtcttttgc cttggcagta tcctgaacgc ccggaagctg
     7321 cacgacgata cggtctgcac ctgactgctg gatgacgggt tcggccacgc ccaactcgtt
     7381 cacacggttg tgcagggtag tgatgttctg tttgaccgca tcggaacgca ctttattgac
     7441 cgcctcttcc gaaagcgtca agacgatatt gctgccgtct gaattcagcg ttgcttcagg
     7501 aaacagcttg cgcaactgcg gcagtgcctt ttgcacatca cctgcatcct gcaaagggac
     7561 ggtcaggctg tttccagcct gacgcaccgt gccgctgcgg attttttcgc ggcgcagttc
     7621 gcggcggatg tcgcccgaat aacgttcaaa cgttttctgc atcgccgctt tcatatcgac
     7681 ctgcatggtg aaatgcacgc cgccgcgcaa gtccaaaccc aaaaacatcg gattggcttt
     7741 gattttctcc atccattcgg ggctgtccgc caacaggttg agcgcggtaa tatacccttc
     7801 gcccaaagtg ttttcgatga cgtcgcgcgc tttaagctgc gtttctgtgt ctttgaaacg
     7861 cactttcagt gaattgtcca caacaaacat cccgtcggtc tgaatacctg cgtttttcag
     7921 cgcggcatcc actttgaatt gagtctgttc gttgatgatg atggcttgtc ggttggtcga
     7981 tacctgcacg gcgggtgttt cgccgaatag gttgggcagc gaatacactg cggcaaccgc
     8041 aatcgtgaac acaatcagca gatatttcca taaaggataa cggttcatca ttgttcctta
     8101 atggttggaa ccccaccctt tcggtggtgt cggaatcggg ctatttcaga agaggcaaaa
     8161 acccttccca gccaggcaag accggaaggc ggcatcttga atatgccgcc ctgcgtgtcg
     8221 gaacatggtc aagccttcgg ttggaattca aaacaaagtg ccgcattcgg gctttccaga
     8281 tgcggcttgt cggcacaaat caatcgactt ttgcggcaat cgcattgcgt tctacttcga
     8341 cctcgatttt tgtaccctgt ccgatatcca cggtaaaaaa ctgttcgccg actctggtta
     8401 ccttaccctt gaaacctgcc gccaagacca ctttgtcgcc gactttcaag gcggcaagca
     8461 ttgcctgatg cgctttgaat ttcttttgct gcggacgcat gatcaggaag tagaacacca
     8521 ccataatcaa cactaaagga gcaaattgtg caacagcttg attcataatt tatccgttct
     8581 ttctaatatg gttgaaaatc gagaggggta tataataaca taagaccgta aacaatatat
     8641 cgggtttgcc gtccgtaccg accgtatacc gcagcctgcc cgtccacaaa cccatgtcct
     8701 tgacccgcct aatcttgaaa ttctatgcac tgttgcgcct ttttttgggc aaaaacgccc
     8761 gcaccgcatg gatttcgcat cccgcctgtg ccgggcacga acccggcgca aaccatcccg
     8821 attcgcccga ccgcatcctc tgcatcgagc aggcattgcg ccgcgccggt atttggcagc
     8881 acctccaaac catagaggcg gaagaaatca gcgatacgcg cctcgcactt gtccactcga
     8941 gcaaatatct gaaccgtttg gaatcttgcc tgccccaaaa aggcaagatt ttccgcctgg
     9001 ataacgacac tgcaatcagc acaggctcgc tgtctgccgc acactttgcc gccggttcgg
     9061 cagttcaggc agtcgacatg gtcatgaacc gtaaagcatg gcatgccttt tgcgccgccc
     9121 gcccgcccgg acaccatgcg ggcagcggca aagccggcgg attctgcctg ctgaacaacg
     9181 ttgccgccgg cgtcatgcat gccattgccg aataccgcct gaaacgcatt gccgtcatcg
     9241 atttcgatgt ccactacggc gacggtacgg cagaaatatt caaagacgat ccgcgcatcc
     9301 tgtttttcaa cctgtttgaa accgaccttt tccccttccc cgaaaacaac gatatgcccg
     9361 acggcggcaa tatggtgcac ctgcccttgc cgccgggaac gggcagccgc acattccgcg
     9421 aagccgtccg cagacagtgg ctgccccggc ttgccgcatt caaacccgaa ctggtgctgc
     9481 tgtcggcagg attcgacgca caccgtctag acgaatcggg caggctcaac ctgcacgagg
     9541 cggattttgc ctggctgaca cacaaaatca ttcagacggc atcgggctgc cccggcaaaa
     9601 tcgtatccgt actcgaaggc ggctacaccc ttgaacctct ggcacaatct gctgccgagc
     9661 atatccgcgt gctggcgggg ctgggcaaat ccgatgccgc aaccgcctat cagaaaacac
     9721 tggatccaac aaaaaaacgg tttgccaaac cgaaaaccgg acaggtgcgc caacctaccc
     9781 aatccgaccg ttac
//
LOCUS       DAXHSF010000062         9767 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.062, whole genome shotgun sequence.
ACCESSION   DAXHSF010000062 DAXHSF010000000
VERSION     DAXHSF010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9767
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.062"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(312..545)
                     /locus_tag="ACFM8S_001102"
     CDS             complement(312..545)
                     /locus_tag="ACFM8S_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HFC6314180.1"
                     /translation="MKLTLMFREYCSLCHKMRDALKPFQNEYGFGLEVVDVDENPVLE
                     EKYNELVPVLLAGDEEICHWFLDEDRLKQFLER"
     gene            complement(563..697)
                     /locus_tag="ACFM8S_001103"
     CDS             complement(563..697)
                     /locus_tag="ACFM8S_001103"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314181.1"
                     /translation="MPSELFRRHFCCFWQDLSIIFLENIVFSQIDHDSAASKALACAN
                     "
     gene            complement(711..911)
                     /locus_tag="ACFM8S_001104"
     CDS             complement(711..911)
                     /locus_tag="ACFM8S_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFC6314182.1"
                     /translation="MFVCICNAVTDHQIKETIAAGATTMGDLQSQLGVASCCGCCGEL
                     AASFLTAHNAQPTVTAGINVQA"
     gene            complement(1083..1958)
                     /gene="xerD"
                     /locus_tag="ACFM8S_001105"
     CDS             complement(1083..1958)
                     /gene="xerD"
                     /locus_tag="ACFM8S_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFC6314183.1"
                     /translation="MEEGLIDRLLETLWLDRRLSQNTLNGYRRDLEKIARRLSQSGRM
                     LKDADEADLAAAVYVDGEQRSSQARALSACKRLYIWMEREGIRTDNPTRLLKPPKIDK
                     NIPTLITEQQISRLLATPDTDTPHGLRDKALLELMYATGLRVSEAVGLNFGSVDLDRG
                     CITALGKGDKQRMIPMGQESAYWVERYYTEARPLLLKGRNCDALFVSQKKTGISRQLA
                     WMIVKEYASQAGIGHISPHSLRHAFATHLVRHGLDLRVVQDMLGHADLNTTQIYTHVA
                     NVWLQGVVKEHHSRN"
     gene            complement(2010..2450)
                     /locus_tag="ACFM8S_001106"
     CDS             complement(2010..2450)
                     /locus_tag="ACFM8S_001106"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HFC6314184.1"
                     /translation="MDVKYEFTLPSSSGADFHSAEHLPLVVYFYPKDSTPGCTTEGLD
                     FNARLEQFEALGYTVVGISRDGVKAHQNFCAKQGFRFELLSDKDETVCRLFDVIKLKK
                     LYGKESLGIERSTFVLNKDGEIAHEWRKVKVAGHAQEVLETLSR"
     gene            2771..3709
                     /locus_tag="ACFM8S_001107"
     CDS             2771..3709
                     /locus_tag="ACFM8S_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFC6314185.1"
                     /translation="MSIHTLKRLPSSLLLGLCLSLPSAHLFADNDILGQFLEQNMLTS
                     SDPIEIFAESTIHPTNTQAITGGLILSSQSALVVNNKTGQILYQKNADRIMPIASISK
                     LMSAMVVLDANLDMNETVTITPDEIDRIKGTGSRLAIGTALTRKELLHLSLMSSENRA
                     THALGRTYPGGMGAFVAAMNRKAQSLGMYGSRFYEPTGLNFQNVSTAKDLSLMVNAAA
                     QYPQIRTNSTSNYASVQTKNGQQNYKNSNALVREGMWNIELQKTGYIREAGRSMVVKA
                     NIQNQPVTIVLLNSPTSATRVNDARKIESWMLQQRS"
     gene            3838..4131
                     /gene="hfq"
                     /locus_tag="ACFM8S_001108"
     CDS             3838..4131
                     /gene="hfq"
                     /locus_tag="ACFM8S_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFC6314186.1"
                     /translation="MTAKGQMLQDPFLNALRKEHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTSVTQMVYKHAISTIVPARSVNLQHENRPQAAPTSTLVQVETVQQPAE"
     gene            4216..4782
                     /locus_tag="ACFM8S_001109"
     CDS             4216..4782
                     /locus_tag="ACFM8S_001109"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6314187.1"
                     /translation="MLLQNILPFAHCLLRKALPEGGNALDGTAGNGHDTLFLAQTAGI
                     RGKVWAFDIQPQALSRTRCRLKEARYENVQLILDGHENLKQYVSEPLNAAIFNFGWLP
                     GGDKSLTTRTETSIAALSATLSLLKENGMLIAVLYPGHENGKQEAEAVGQWAKNLPQE
                     QFAVLRYGFTNRKNSPPYLLAFEKLRQK"
     gene            4836..5210
                     /locus_tag="ACFM8S_001110"
     CDS             4836..5210
                     /locus_tag="ACFM8S_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HFC6314188.1"
                     /translation="MQYLIVKYSHQIFVTITILVFNIRVFLLWKNPEKPLVGFWKALP
                     HLNDTMLLFTGLWLMKITHFSPFNAPWLGTKILLLLAYIALGMMMMRARPRSTKFYTV
                     YLLAMCCVACIVYLAKTKVLPF"
     gene            5220..5714
                     /gene="folK"
                     /locus_tag="ACFM8S_001111"
     CDS             5220..5714
                     /gene="folK"
                     /locus_tag="ACFM8S_001111"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFC6314189.1"
                     /translation="MNNRHFAVIALGSNLENPAQQVRAALDTLSSHPDIRLHQTSSLY
                     ATAPVGYDDQPDFVNAVCTVSTDMDSISLLSLLNRIEADFGRKRSFRNAPRTLDLDII
                     DFDGISSDDPRLTLPHPRAHERSFVIRPLAEILPDFVLGKHGKVAELSKQLGNQGIRL
                     LPDR"
     gene            complement(5840..6319)
                     /locus_tag="ACFM8S_001112"
     CDS             complement(5840..6319)
                     /locus_tag="ACFM8S_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HFC6314190.1"
                     /translation="MPESIFKQISLDILKLHRDSVYSLLATSGCNCQVHEAAYVNIDG
                     KYYIALSCEPEVGEVKTGILLIEDESRSLRLSWVGSARELDRKDSAYKRALSALSRKM
                     GRCKDKLHTAVQPFLLELVPEKGRFSVGDEEVWISRNDLVRALYPVGYSMRQAVLQI"
     gene            complement(6539..7276)
                     /gene="ubiE"
                     /locus_tag="ACFM8S_001113"
     CDS             complement(6539..7276)
                     /gene="ubiE"
                     /locus_tag="ACFM8S_001113"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFC6314191.1"
                     /translation="MGGQKTHFGFSTVNEDEKAGKVAEVFHSVAKNYDIMNDVMSAGL
                     HRVWKHFTINTAHLKKGDKVLDIAGGTGDLSRGWAKRVGKEGEVWLTDINSSMLTVGR
                     DRLLNEGMILPVSLADAEKLPFPDNYFNLVSVAFGLRNMTHKDAALKEMYRVLKPGGT
                     LLVLEFSKIYKPLEGAYDFYSFKLLPVMGKLIAKDAESYQYLAESIRMHPDQETLKQM
                     MLDAGFDSVDYHNMSAGIVALHKGVKF"
     gene            complement(7319..7687)
                     /locus_tag="ACFM8S_001114"
     CDS             complement(7319..7687)
                     /locus_tag="ACFM8S_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HFC6314192.1"
                     /translation="MNNIPVEIRLLKNRTVLVLTYGDEPKNLPAEFLRVYSPSAEVRG
                     HGVGQDVLQTGKADVQITDLQPVGQYALKISFSDGHDSGLYDWAYLHRLAYGYDAMWQ
                     EYLDKLAAAGASRFEKQSDC"
     gene            7807..9426
                     /gene="mnmC"
                     /locus_tag="ACFM8S_001115"
     CDS             7807..9426
                     /gene="mnmC"
                     /locus_tag="ACFM8S_001115"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFC6314193.1"
                     /translation="MDNLVWDGVPDIRTLDQTIRKHAHPLNLIVCLPDNQIPDFQTAQ
                     DASDAECRLKHRLDQATQCLQFDSINLIEHILPDVRFWLVPPSRTRRLHEHFHHISWQ
                     TEAIPQTESKPDKPWFALPQTSERQKPEYILVIGAGISGAATAHALASHGISVTVLEA
                     RKAAQAASGNRQGLLYAKISPHDTEQTELLLAGYGYTKRLLGHILPESDTWGGNGIIH
                     LNYSRIEQQRNHELGLQKHHNHLYRSITSAEAEKIAGIPLSVPYDHPSCGLYWQHGVW
                     LNPPAFVRALLNHPLIGLHEDTPLTDISHDGEKWIASTPNGIFSATHIIYCTGAHSSC
                     LPETNLAALPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNS
                     SHTGWNEAEEASNRQALAQLSPALAESLFAANPIPQKHQGHAAMRCDSPDHLPLVGAL
                     GDIAAMQQTYAKLALDKNYRIDAPCPYLPNAYANTAHGTRGLATAPICAAAVAAEILG
                     LPHPLSKRLRHALHPNRTIIRAIVRRKDLTP"
     gene            complement(9540..9657)
                     /locus_tag="ACFM8S_001116"
                     /pseudo
     CDS             complement(9540..9657)
                     /locus_tag="ACFM8S_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain protein"
BASE COUNT         2612 a         2983 c         1954 g         2218 t
ORIGIN      
        1 taccgattac acaaccacat atcatcttta cacaatcatg cttccatcaa cagcaaaaca
       61 tgatatgatt gctgctaata acatctcaca ataaattttc taatttttat tgaaaaaatc
      121 aataaataag aattctccca accgacaaat aaaataaaga aagggtcaat atgcaacatc
      181 gaagaagact ggcaatttac caggcatcca aacgtgcttc ctttaccggc aggtcatccg
      241 ccccgcaaaa acgtaaagaa cgttgatttg aaaaaaatgc cgcctgaagt cctgcttcag
      301 acggcatttt tttaccgttc gagaaactgt ttcaacctgt cctcatccaa aaaccagtgg
      361 cagatttcct catctcccgc caacaaaacg ggaaccagct cattgtattt ttcttccaaa
      421 acaggatttt catccacatc gaccacttcc agcccgaacc cgtattcatt ttgaaaaggt
      481 ttgagtgcat cgcgcatttt gtggcacaag ctgcaatatt cacgaaacat caaggtcaat
      541 ttcatccgcg tttccttatc tgtcaatttg cacacgccaa agccttagac gcagcagaat
      601 catggtctat ttgggaaaaa acaatgtttt caaggaagat gatactcaag tcctgccaaa
      661 aacagcaaaa atgccgtctg aacagttcag acggcatttc caaaaccgtt ttacgcttga
      721 acattgatac ccgccgtaac cgtcggttgc gcattgtgcg ccgtcagaaa cgaagcggca
      781 agctccccac agcagccgca gcagctcgct acgcccaatt gcgactgcaa atcgcccatt
      841 gtggtcgcgc cggcggcgat ggtttccttg atttgatggt cggtaacggc attgcagatg
      901 cagacaaaca ttttgtgctc cgtgtgttct caaactatcg taccgatagc ggcttttatt
      961 atcgtatgca aatataaata aaaacggttc gcattgcaag gtcggcatac acggtttgtc
     1021 ttggtaattt tttatcaagt tttcatttcc gattgatttc ttattaaacc gaagcagaag
     1081 cctcagtttc gggaatggtg ttccttcact acaccctgca accatacgtt ggcaacatgg
     1141 gtataaatct gcgtcgtatt caaatcggca tgtcccaaca tatcctgaac cacgcgcaaa
     1201 tccaagccgt gccgcaccag atgcgtggca aaggcgtggc gcaggctgtg cgggctgatg
     1261 tgcccgatgc ctgcctgact tgcatattct ttgacaatca tccatgccaa ctgacgggaa
     1321 atgcccgtct ttttctgact gacaaacaat gcgtcgcaat tcctgccttt cagcagaagt
     1381 gggcgtgcct ccgtataata gcgttccacc caatacgccg actcctgccc catcgggatc
     1441 atcctctgct tgtcgccctt gcccagcgcg gtaatacagc ccctgtccaa atccacactg
     1501 ccgaaattca gcccgaccgc ctcgctgacg cgcaagccgg tcgcgtacat caattcgagc
     1561 agtgccttgt cccgcaaacc gtgtggcgta tcagtatccg gagtggcaag cagccgggaa
     1621 atctgctgtt ctgtaatcag tgtcggaata ttcttgtcga ttttgggcgg tttcagcaaa
     1681 cgggtgggat tgtccgtcct tatgccttca cgctccatcc atatatacag gcgtttgcac
     1741 gccgataatg cgcgcgcctg cgaactccgt tgctctccgt caacataaac cgccgccgcc
     1801 aaatccgctt cgtccgcatc cttcagcatt ctgcccgatt gggacaggcg gcgggcgatt
     1861 ttttccaaat cgcgccggta accgtttaaa gtattctgac tgagccgcct gtccaaccac
     1921 agcgtttcaa gcagcctgtc gatcaaacct tcttccatac cgttccaaac aaatgccgtc
     1981 tgaatcttct tcaaacggca tggttcacat tatcgggaaa gcgtttccaa tacttcctgc
     2041 gcgtgacccg ccactttgac tttccgccat tcatgggcga tttctccatc cttattcaag
     2101 acgaacgtac tgcgctcgat acctaacgac tctttcccgt acagtttctt caatttgatg
     2161 acatcaaaca ggcggcacac tgtttcatcc ttgtcgctca acagctcgaa ccggaaaccc
     2221 tgcttggcgc aaaaattctg atgcgccttt acgccgtcgc gggaaatacc gaccacggta
     2281 taacccaatg cctcaaactg ttccaaacgc gcattgaaat ccaagccttc cgtcgtacag
     2341 cccggcgtac tgtctttcgg ataaaaatac acgaccaaag gcagatgttc tgccgaatga
     2401 aaatccgcac cgctgctcga aggcagggta aattcatatt tcacatccat agtcctactc
     2461 ccgatattcc cattattcaa aacgacacgc agacgcaccg ccgcaattgc caaaccaacc
     2521 ccgattctac cgccccaaag gacagggatt caaccgccgg aaacatccaa accgacacac
     2581 gacggcatga aaaatatcca tgtcaaacca caaaatatgt tccgatttaa aaacagaatg
     2641 ttataaaacc caatccccaa aacacaacaa gaccgcccgc ctacgggcag tctcctgtca
     2701 gacgacatac tttacagatg gctgtttttt caacaaaata acgccaatac tcaaaaatat
     2761 ggaatcaaaa atgtccatcc atactctgaa acgcctgccc tcatcgctgc tgctcggtct
     2821 ctgcctttcc ctgccgtcag cccacctttt tgccgacaac gacattttag ggcaattttt
     2881 agaacagaac atgcttacct cctccgatcc gatagaaata ttcgccgaaa gcacgataca
     2941 ccccaccaac acccaagcca ttacaggcgg tctgattctc tcctcacagt ctgccctggt
     3001 cgtcaacaac aaaaccggac agatactgta tcagaaaaac gccgacagga ttatgcccat
     3061 cgcctccatt tccaaactga tgagcgcgat ggtcgttttg gatgcaaact tggacatgaa
     3121 cgaaaccgtt accattacgc ccgacgaaat cgaccgcatc aaagggaccg gcagccgtct
     3181 tgccataggt acggcactta cacgcaaaga actgctgcac ctgagcctga tgagcagcga
     3241 aaaccgcgcc acccatgcat tgggcagaac ctaccccggc ggcatgggcg catttgtcgc
     3301 cgccatgaac cgcaaagccc aaagcctcgg tatgtacggc agccgctttt acgaaccgac
     3361 cggactcaac ttccaaaacg tttctaccgc caaagacttg agccttatgg tcaacgccgc
     3421 cgcccaatat ccgcaaatcc gcaccaactc gacttccaac tacgcctcgg tacagaccaa
     3481 aaacgggcag cagaactaca aaaactccaa tgccctggtc agagaaggca tgtggaacat
     3541 cgaattgcag aaaaccggct acatacgcga agcaggcagg tctatggttg tcaaagccaa
     3601 cattcaaaat caacccgtta ccatcgtatt gctgaactcg cccacatccg ccacacgcgt
     3661 caacgacgcc cgcaaaatcg aatcgtggat gctgcagcaa cgctcctgac atacaaatgc
     3721 ccggcggaaa accgcccaaa cacaaaaaaa attggtaatt tttcctattc aaggctacaa
     3781 ttcgacacgc acagggcgtc tgcccgattt cacaatcata atagcggagt taaaaatatg
     3841 acagctaaag gacaaatgtt gcaagatccc ttcctgaacg ccctgcgtaa agagcatgtt
     3901 ccggtttcga tttacttagt taacggtatc aaattacaag gtcaggttga gtctttcgat
     3961 caatacgttg ttctcctgag aaacacttcc gtcacccaaa tggtttacaa acacgccatt
     4021 tccaccatcg taccggcacg ctccgtcaac ctacaacatg aaaacagacc ccaagccgca
     4081 ccgacttcga ccctcgtcca agtggaaacc gtccagcagc ctgccgaata atccgcacga
     4141 agcatgacgt gtcatatctt tcaatacctt accggacaac ggtaaggtat ttttattttc
     4201 agacagcatt taaaaatgtt attgcaaaac atccttccat tcgcccattg ccttttgcgg
     4261 aaggcacttc ccgaaggcgg caatgctttg gacggcaccg ccggcaacgg acacgacacc
     4321 cttttcctcg cacaaaccgc aggcatccgg gggaaagtgt gggcattcga catccagccg
     4381 caagccctga gccgcactcg atgccgtctg aaagaagcga gatacgagaa tgtgcaactc
     4441 atcttagacg gacacgaaaa cctgaaacaa tatgtttcag aaccactaaa tgccgccatt
     4501 ttcaactttg gctggctgcc cggcggagat aaaagcctta ccacccgcac ggaaaccagc
     4561 attgccgccc tttctgccac cttatcccta ctgaaagaaa acggtatgct tattgccgtc
     4621 ctctatccgg gacacgaaaa cggcaaacag gaggcagaag ccgtcggaca atgggcaaaa
     4681 aacctgcctc aagaacagtt tgccgtttta cgttacggct ttaccaaccg gaaaaacagc
     4741 ccaccctatc tgttggcatt tgaaaaactg cgtcaaaaat aactgtttgc ggtaaaataa
     4801 gccgattcta acccttattt tgatacgagc cgaccatgca gtatctgatt gtcaaataca
     4861 gccatcaaat cttcgttacc atcaccattt tggtattcaa catccgtgtt ttcctacttt
     4921 ggaaaaatcc agaaaagccc ttggtcggct tttggaaggc actgccccac cttaacgaca
     4981 ccatgctgct gtttacggga ttgtggctga tgaagattac ccatttctcc ccgttcaacg
     5041 caccatggct cggcacaaaa atcctgcttc tgctcgccta tatcgcattg ggtatgatga
     5101 tgatgcgcgc ccgtccgcgt tcgaccaagt tctacaccgt ttacctgctc gccatgtgtt
     5161 gcgtcgcctg catcgtttac cttgccaaaa ccaaagtcct gcctttctga aacaccgtta
     5221 tgaacaacag acattttgcc gtcatcgccc tgggcagtaa tcttgaaaac cctgcccaac
     5281 aggtacgcgc cgcattggac acgctgtcgt cccatcccga tattcggctt catcaaacct
     5341 cgtccctgta tgccaccgcc cctgtcggct atgacgacca gcccgacttt gtcaatgccg
     5401 tctgcaccgt ttccaccgat atggacagca tctcgctgct ctccttgctc aaccgtatcg
     5461 aggcggattt cgggcgcaaa cgcagtttcc gaaacgcgcc gcgcacattg gatttggaca
     5521 ttatcgactt tgacggcatc tccagcgacg acccccgcct caccctgccg catccgcgcg
     5581 cacacgaacg cagtttcgtc atacgccctt tggcagaaat cctccctgat tttgttttag
     5641 gcaaacacgg aaaagttgcc gaattgtcaa aacaactggg caatcaaggt atccgtcttt
     5701 taccggacag gtaattccgc acacagatgc cgtctgaaag cctttcagac ggcattttta
     5761 ccctgccgcc aatctgtacg caaaaaaaat cgccccttga aaaaggggcg caaaaggaac
     5821 acaaaccact accaaaactt taaatctgca acactgcctg ccgcatactg tatccgacag
     5881 gatataaagc cctcaccaaa tcgtttcgag aaatccaaac ttcttcatcg ccgacagaaa
     5941 acctgccttt ctccggcacc agctccaaca aaaacggctg aaccgccgta tgcagcttgt
     6001 ctttacaccg ccccatcttt ctggacaacg cggacagggc gcgtttgtag gcactatcct
     6061 tgcggtcaag ctcccgcgca ctgccgaccc agctcaaacg caggctgcgg ctttcatcct
     6121 caatcagcaa aatgcctgtt ttgacttccc ccacctcggg ctcgcatgaa agcgcaatat
     6181 aatatttgcc gtcgatgttg acatacgccg cttcatgcac ctgacagttg cagccggaag
     6241 tggcaagcag cgaataaaca gagtcccgat gcagtttcaa aatatcaagg gaaatctgtt
     6301 tgaaaatact ttccggcatt tttccctcct ttcgtctgct gcgttgttta ctggattgac
     6361 tgcattatac agacatctcc caatcttgca aatgataatt gttcctattt tttaaaaaat
     6421 ttataaatta ttgatagata tgtgaattaa ttttaagatt taaaatctct ttctcaaaaa
     6481 aacacaaaaa tgccgtctga aacgtgttca gacggcattg gctgcacagg caatccgttt
     6541 aaaatttcac gcccttatgc agcgcaacga tccccgcact catattatga tagtccacac
     6601 tgtcaaatcc ggcatccagc atcatttgct ttaaagtttc ctgatcgggg tgcatacgga
     6661 tggattcggc aagatattga taactctccg catctttggc aatcagtttg cccatgaccg
     6721 gcagcagctt gaacgaatag aaatcatacg cgccttccaa aggtttgtag attttggaaa
     6781 actccaacac cagcaacgtg ccgccgggtt tcaaaacacg gtacatctct ttcagcgcgg
     6841 catctttatg cgtcatgttc cgcaagccga acgccacgga aaccaagttg aaataattgt
     6901 cagggaaagg cagtttttcc gcatcggcaa gcgacacggg caaaatcatg ccttcgttca
     6961 aaagacggtc gcgcccgacg gtcagcatag aggaattaat atcggtcagc caaacctcgc
     7021 cttccttgcc gacccgtttc gcccaaccgc gcgacaaatc gcccgtaccg ccggcaatgt
     7081 ccaacacttt atcgcctttt ttcaggtgcg ccgtgttgat ggtgaaatgc ttccacaccc
     7141 tgtgcagccc tgccgacatc acatcgttca taatgtcgta gtttttggcg acggagtgga
     7201 acacttccgc cacttttccg gctttctcat cttcgttgac cgtgctaaac ccgaaatgcg
     7261 ttttttgtcc gcccatattc taaccacctc cctgataccg ccctacggca tcccaaactt
     7321 aacaatctga ttgcttttca aaacgggaag cacccgccgc cgccaatttg tccaaatatt
     7381 cctgccacat cgcatcgtat ccgtatgcca gtctgtgcag atacgcccaa tcgtaaagac
     7441 cgctgtcgtg cccgtccgaa aaactgattt tcagcgcgta ctgtccgaca ggctgcaaat
     7501 ccgtgatttg gacatccgcc ttgccggtct gcaaaacatc ctgtcccaca ccgtgtccgc
     7561 gcacttccgc actcggcgaa tagacgcgta aaaattcggc aggcagattt ttaggttcgt
     7621 ccccataagt caaaaccaac acggtacggt ttttcaacaa acggatttca acaggaatat
     7681 tattcaaaaa acacctcggt tcggcataca atatccggca ggcgtacgcg tgcctttaaa
     7741 cggcgcgtat tttaacagct ttatcccgat accgatattc aaacccacac tttaaacggc
     7801 attgatatgg acaatctcgt atgggacggc gttcccgaca tccgcacact cgaccaaacc
     7861 atccgcaaac acgcacaccc gctcaacctg attgtctgcc tccccgataa tcagattccc
     7921 gattttcaaa ccgcacaaga tgcttcggac gcggaatgcc gtctgaagca ccgtttggat
     7981 caggcaacgc agtgcctcca gttcgacagc atcaacctca tcgaacacat cctgcccgat
     8041 gtccgcttct ggctggttcc cccttcacgc acccgccgcc tgcacgaaca cttccaccac
     8101 atttcctggc agaccgaagc catcccgcaa accgaaagca aacccgacaa accctggttt
     8161 gcacttccac aaacatccga acggcaaaaa ccggaataca tcctcgttat cggcgcgggc
     8221 atatccggcg cggcaaccgc ccacgcctta gcatcacacg gcatttccgt taccgtattg
     8281 gaagcccgaa aagccgccca agccgccagc ggcaaccgcc aagggctgct ctacgccaaa
     8341 atctcgccgc acgacaccga acaaaccgaa ctgctgcttg ccggctacgg ctacaccaaa
     8401 cgcctgctcg gacacatcct gcccgaatcc gacacttggg gcggcaacgg catcatccac
     8461 ctcaattaca gccgcatcga acaacaacgc aatcacgaat tgggtttgca aaaacaccat
     8521 aaccacctct accgcagcat cacgtctgca gaagccgaaa aaatcgccgg tatcccactg
     8581 tccgtcccat acgaccaccc ttcatgcgga ctctactggc aacacggcgt atggctcaat
     8641 ccacccgcat tcgtccgcgc cctcctcaac catccgctca ttggactaca cgaagacaca
     8701 ccgttaaccg acatttccca cgacggggaa aagtggattg caagcacgcc aaacggcata
     8761 ttttccgcca cacacatcat ctactgcacc ggcgcgcaca gctcctgcct gcccgaaacc
     8821 aacctcgccg ccctgcctct caggcaaata cgcggacaaa ccggcctcac accatccacc
     8881 ccgttttccg aacaactgcg ttgcgccgtt tcaggcgaaa gctacatcag cccgtcgtgg
     8941 cacggactgc actgctacgg cgcgagtttt attcccaaca gcagccatac cggatggaac
     9001 gaagccgaag aagcctcaaa ccgccaagca ttggcacagc ttagccccgc cctcgccgaa
     9061 tcattgtttg ccgccaaccc aatcccccaa aaacaccaag ggcacgccgc catgcgctgc
     9121 gacagccccg accaccttcc cctagtcggc gcactcggcg acattgccgc tatgcaacaa
     9181 acttacgcca aactcgcgct ggacaaaaac tatcgcatcg acgccccctg cccgtacctg
     9241 cccaatgcct acgccaacac cgcccacggc acacgcgggc ttgccaccgc ccccatctgc
     9301 gccgccgccg ttgcagccga aatcctaggc ttgccccatc ccctctcaaa acgcctgcgc
     9361 cacgccctac accccaaccg caccatcatc cgcgccatcg tcagaaggaa ggatctaacc
     9421 ccttaagcct ttgccgcatg ccgtcccgac cacagaaaat catattcaaa taaacatacc
     9481 aatcctgacg gtaaaatatc gaaaacaccc ataacgtttt cttcccaaaa taaggagtaa
     9541 ggaatgccta acaaattgcc tttgctgtcc aagatgaata ttccgtaaca ttcgaagact
     9601 tccttaaccc tgccagccgt ttcggtttct gtaccccctg ttagataagg aaggaatgaa
     9661 aaagccctgt tgattgtatg aagtaaaggg cgttaatttt tgttaatctt cccttctcag
     9721 gaactcaata tataaggatg ttaattttta tttcccttct cgaggac
//
LOCUS       DAXHSF010000063         9530 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.063, whole genome shotgun sequence.
ACCESSION   DAXHSF010000063 DAXHSF010000000
VERSION     DAXHSF010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9530
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.063"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(235..1677)
                     /gene="aldA"
                     /locus_tag="ACFM8S_001117"
     CDS             complement(235..1677)
                     /gene="aldA"
                     /locus_tag="ACFM8S_001117"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFC6314194.1"
                     /translation="MKQLAMYINGRFENDFNGEWRDVLNPSTEETIAREPKGGKADVD
                     RAVAAARAAQPAWERLPAVERGAYLRKIAQGIRGRADELTDTIVAEGGKTKDLARVEV
                     MFTADYLDYQAEWARRYEGEIIQSDRPRENILLFKRPLGVIAGILPWNFPFFLIARKM
                     GPALVTGNTIVVKPSSVTPINCHIFAEIVDAVGLPAGVFNVVNGPGAEIGNALSAHPQ
                     VDMVSLTGSVEAGRQVMEAASANITKVSLELGGKAPAIVLKDADLDLAVKSILASRVG
                     NTGQICNCAERVYVHSSLKDAFIEKMTAAMKGVRYGNPAEAEAGALEMGPLIEERAVK
                     AVAEKVERAVKQGAKLVCGGKRAEGRGYFFEPTLLTDTDNSMDIMKEETFGPVLPVSA
                     FDTLDQVIALANDCEFGLTSSVYTTNLNEAFYVTRRLQFGETYINRENFEAMQGFHAG
                     WKKSGIGGADGKHGLEEYLQTQVVYLETDI"
     gene            complement(1712..1912)
                     /locus_tag="ACFM8S_001118"
     CDS             complement(1712..1912)
                     /locus_tag="ACFM8S_001118"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314195.1"
                     /translation="MDTVGSLAIGYATGGNAGAFTDANADWNNRQLHSSEYKLAEQYA
                     DIVASRLNISKETCTAIYLGIN"
     gene            complement(1921..2310)
                     /locus_tag="ACFM8S_001119"
     CDS             complement(1921..2310)
                     /locus_tag="ACFM8S_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314196.1"
                     /translation="MLYKCKIKKLDNTIEEAVLIEINGYELHCFISYCPFKIFENQFY
                     EVELSYEIFNDHILDKSTSNIGFRKIDDSFKYEITAELKNNHLTLQNIQFEEDEYLVN
                     YSYLDNCLVTLTADRISVSFIREIQAA"
     gene            complement(2519..3436)
                     /locus_tag="ACFM8S_001120"
     CDS             complement(2519..3436)
                     /locus_tag="ACFM8S_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6314197.1"
                     /translation="MEKAEHLNSSRFVNLVKSGGGSYVEGSYRFDTLSNGISIHGGTV
                     TARCDFCSSRLAEPYVSFVLLLEGSLDFGINRSRFQIDADGGKVILISVGEEVLFSRY
                     LYRGGKTVKMTIKGMEQWLLRPEYARFAPLLYREPVRIWGLPPNLRGLAASCLKAVPK
                     GHLGETLRREADVLRLLSDLWDTVSDGIGPAAGQTAEADAMPSEDFSRTLNAAFADGA
                     HQVNRLTDALNISERTLQRRMRDHFGITASEWLHHKQMQHALYLLQNEGKSIGETAYL
                     CGYRHVSSFTQAFRQYFGSTPAETKKENR"
     gene            3609..4409
                     /locus_tag="ACFM8S_001121"
     CDS             3609..4409
                     /locus_tag="ACFM8S_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC6314198.1"
                     /translation="MSPSPFIEMKDVAFAYGDRPILKNINFSIPQGNFAAVMGGSGSG
                     KTTLMRLITGQIRPQSGQVLIEGRDLAGFSADELYEHRRRMGVLFQHGALFTDLSVFD
                     NIAFPMRELTRLPEAVIRDLVLLKLNAVGLRGVENLMPSELSGGMSRRVALARTIALD
                     PEIMLYDEPFTGLDPISLGVIAHLISRVNKALRSTSIMVTHDIEKSLEIVDQVIFLAH
                     GEIMFSGSPQEMRELDSPWVRQFVGGLADGPVAYRYPAQTSLQQDLLG"
     gene            4457..5233
                     /gene="mlaE"
                     /locus_tag="ACFM8S_001122"
     CDS             4457..5233
                     /gene="mlaE"
                     /locus_tag="ACFM8S_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFC6314199.1"
                     /translation="MNFIRSVGAKTLGLIQSLGSITLFLLNILAKSGTAFVRPRLSVR
                     QVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLA
                     AILFASSAGGAMTSEIGLMKTTEQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
                     NVAGIFGAYLVGVSWLGLDSGIFWSQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQ
                     GFHCVPTSEGILRASTRTVVSSALTILAVDFILTAWMFTD"
     gene            5284..5778
                     /gene="mlaD"
                     /locus_tag="ACFM8S_001123"
     CDS             5284..5778
                     /gene="mlaD"
                     /locus_tag="ACFM8S_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFC6314200.1"
                     /translation="MKKNILEFWVGLFVLLGVAAVGFLAFRVAGGAAFGNSGKTYTVY
                     ADFSDIGGLKANAPIKSAGVLVGRVGSIELDPKSYQAKVRLDLDSKYQFSSDVSAQIL
                     TSGLLGEQYIGLQQGGDTENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNADGGNAE
                     KAAE"
     gene            5815..6405
                     /locus_tag="ACFM8S_001124"
     CDS             5815..6405
                     /locus_tag="ACFM8S_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFC6314201.1"
                     /translation="MKKSSLISALGIGILSIGMAFAAPADAVSQIRQNATQVLSILKN
                     GDANTARQKAEAYAIPYFDFQRMTALAVGNPWRTASDTQKQALAKEFQTLLIRTYSGT
                     MLKLKNANVNVKDNPIVNKGGKEIIVRAEVGVPGQKPVNMDFTTYQSGGKYRTYNVAI
                     EGASLVTVYRNQFGEIIKAKGVDGLIAELKAKNGGK"
     gene            6456..6734
                     /locus_tag="ACFM8S_001125"
     CDS             6456..6734
                     /locus_tag="ACFM8S_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFC6314202.1"
                     /translation="MHTELKNGILHIGGDITVKTLTAAAFTRFRQQCRLKDTCAVDLS
                     GVGRADSACVSLLLEVLRGCKGSVRLTGIPESVRALSELYEIKDWLKS"
     gene            6731..7558
                     /locus_tag="ACFM8S_001126"
     CDS             6731..7558
                     /locus_tag="ACFM8S_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HFC6314203.1"
                     /translation="MKKTAYAFLLLIGFASAPAFAETRPADPYEGYNRAVFKFNDQAD
                     RYIFAPAARGYRKVAPKPVRAGVSNFFNNLCDVVSFGSNILRLDIKRASEDLVRVGIN
                     TTFGLGGLIDIAGAGGIPDNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGIT
                     SVYSPKNIVFRTPVGRWGTTAVSAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRA
                     RQTGATPAEGTEDNIDIDELVESAETGAAETAVQEDSVSETQAEAAGEAETQPGTQP"
     gene            7683..8045
                     /locus_tag="ACFM8S_001127"
     CDS             7683..8045
                     /locus_tag="ACFM8S_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HFC6314204.1"
                     /translation="MYEVNRSVFVLIPLEPFWNWLQTLPGNHLDGLTLEDIQADANSY
                     LVRPCETADEVWDEIEARFEDIFAAELADWCEDEREWPALDADIFNEWFDIQLSTVIT
                     DLEHEPLAREAFQPINLN"
     gene            8045..8428
                     /locus_tag="ACFM8S_001128"
     CDS             8045..8428
                     /locus_tag="ACFM8S_001128"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFC6314205.1"
                     /translation="MKLTVRNYHLDGYGHVNNARYLEFFEEARWAFFEERGLLHELAG
                     LILIVARIDIRYSRPAVEGDVLQFSCRLKTPGTRRIVLTQTITLPNGKTAAEADITLM
                     PVHAATQRTVSLPATLARALEALSE"
     gene            8425..8913
                     /locus_tag="ACFM8S_001129"
     CDS             8425..8913
                     /locus_tag="ACFM8S_001129"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HFC6314206.1"
                     /translation="MKKILTAAVVALIGILLAIVLIPDSKTAPAFSLPDLHGKTVSNA
                     DLQGKVTLINFWFPSCPGCVSEMPKIIKTANYYKNKNFQVLAVAQPIDPIESVRQYVK
                     DYGLPFTVMYDADKAVGQAFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTAL
                     AQ"
     gene            complement(8996..9124)
                     /locus_tag="ACFM8S_001130"
     CDS             complement(8996..9124)
                     /locus_tag="ACFM8S_001130"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFC6314207.1"
                     /translation="MAGVTVGRGSVVGAGAVVTKDIPPYSLAAGNPAVVKKNLPEG"
     gene            complement(9130..9510)
                     /locus_tag="ACFM8S_001131"
     CDS             complement(9130..9510)
                     /locus_tag="ACFM8S_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-o"
                     /protein_id="HFC6314208.1"
                     /translation="MGSKFFFLLLRFAGSGLPPSHMRGIGIVGRRVRGFLARRVSPHI
                     ERGVNIERGAYVFPDTVLGDGSGIGANCEICRGPVVGKNVMMEPECLFYSNNHKFNRS
                     KNALRATRKSVRLRWRTMSGRGTG"
BASE COUNT         2208 a         2652 c         2386 g         2284 t
ORIGIN      
        1 gtacggcgtt gcctcgactt agctcaaaga aacgattctc taaggtgctc aagcaccgag
       61 tgaatcggtt ccgtactatt tgtactgtct gcggcttcgt ttaaatttaa tccgctatac
      121 aaagacaaaa acatcgatgt cggtcatgac tgattgctgg tgaaactgta aaaatgccgt
      181 ctgaacctga aaacgggttc agacggcatt ttctatcggg gttttaaagc ggcattaaat
      241 gtcggtttcc agataaacga cttgggtttg cagatattct tccaaaccgt gtttgccgtc
      301 cgcgccgccg ataccggatt ttttccaacc ggcgtggaaa ccctgcatcg cttcaaagtt
      361 ttcgcggttg atgtaggttt cgccgaattg caggcggcgg gtaacgtaga aggcttcgtt
      421 taaattagtc gtataaacag aactggtcag accaaactcg caatcgttag ccagtgcgat
      481 gacctggtcg agcgtgtcga aagcggaaac gggcagcacg gggccgaagg tttcttcttt
      541 cataatgtcc atactgttgt cggtgtcggt cagcagggtc ggctcgaaga aataaccgcg
      601 tccttcggcg cgtttgccgc cgcaaaccaa tttcgcacct tgtttgactg cccgttccac
      661 tttttcggca acggctttga cggcgcgctc ttcaatcagc gggcccattt ccagcgcgcc
      721 tgcttcggct tcggcagggt tgccgtagcg tacgcctttc atggcggcgg tcattttttc
      781 aatgaatgcg tctttcagac tgctgtggac ataaacgcgc tcggcgcagt tgcagatttg
      841 accagtgttg ccgacgcgcg aagccaagat ggatttcact gccaagtcca aatccgcgtc
      901 tttcaaaacg atagcaggtg ctttgccgcc gagttccagc gagactttgg tgatgttggc
      961 agaagcggct tccatcactt ggcggcccgc ttcaacggag ccggtcaggc tgaccatatc
     1021 gacttgcgga tgggcggaca aggcattgcc gatttccgca cccggaccgt tcacaacgtt
     1081 gaacacgcct gctggcagtc cgaccgcatc gacgatttcg gcgaagatgt ggcagttgat
     1141 cggggttacg ctgctgggtt tgacgacgat ggtgttgccc gttaccaaag cggggcccat
     1201 tttgcgggca atcaggaaga aggggaagtt ccacggcaaa atgccggcga ttacgcccag
     1261 cggacgtttg aacaataaaa tattttcgcg cgggcggtcg ctttggatga tttcgccttc
     1321 gtaacgacgc gcccattcgg cttggtaatc gagatagtcg gcggtaaaca tgacttccac
     1381 gcgcgccaag tctttggttt tgccgccttc ggcaacgatg gtgtcggtca gctcgtcggc
     1441 gcgtccgcgt atgccttggg cgattttgcg caaatacgcg ccgcgttcga ccgcaggcag
     1501 gcgttcccaa gccggttgcg ccgcgcgtgc cgccgccaca gctcggtcaa cgtctgcttt
     1561 tccgcctttg ggttcgcggg cgatggtttc ttcggtggat gggttcaata cgtcgcgcca
     1621 ttcgccgttg aaatcgtttt caaagcgtcc gttgatgtac atggccaatt gtttcatttc
     1681 gggttctcca gttttgtagt cagatgtagt cttagtttat tcccaaataa attgccgtac
     1741 aagtttcttt tgaaatgttt agacgagaag ctactatgtc tgcatattgt tctgctaatt
     1801 tatattcact cgaatgcaac tgccgattat tccaatctgc attcgcatca gtaaaagccc
     1861 cagcattgcc gcctgtggca taaccaatcg ctaaactgcc aacggtatcc acaacaggtt
     1921 tcaggctgcc tgaatttctc taataaatga aacacttatt ctatcagccg ttaatgtaac
     1981 caaacaatta tcaagataag agtagtttac taaatactca tcttcttcaa attgtatatt
     2041 ttgtagggtt agatgattat tttttaattc tgccgttatt tcatacttga aactatcatc
     2101 tatttttcta aatccaatat ttgaagttga tttatccagt atgtgatcat taaagatttc
     2161 ataggataat tccacttcat aaaattgatt ttcaaatatt ttgaatgggc agtatgatat
     2221 aaaacaatgt agttcataac catttatttc aatcaatact gcctcttcaa tggtgttatc
     2281 taactttttg attttacatt tatataacat tttatcttgc ctttagataa attgttttgt
     2341 tttttggatt gttattcaaa gttctccaaa agtcttttcc tgattttgta tgtatcgtgg
     2401 taacactacc attttcattg gttccaacaa aaataaattt agattgacct ttggaatcag
     2461 tacccaaaaa ctgaacatat ccagacatgc agatttcggg ttttaaatca aatgcggctt
     2521 accggttttc ttttttggtt tccgccggcg tgctgccgaa atattgcctg aatgcctgag
     2581 taaagctgga aacgtggcgg tagccgcata aatatgcggt ttcgcctatg cttttcccct
     2641 cgttttgcaa caggtagagc gcgtgctgca tttgtttgtg gtgcagccac tcgcttgcgg
     2701 taataccgaa atggtcgcgc atacggcgtt gcagcgtcct ttcactgatg ttcagcgcgt
     2761 ctgtcagccg gttgacttgg tgtgcgccgt cggcaaacgc ggcatttagg gtgcggctga
     2821 agtcttcaga cggcatagcg tctgcttccg ccgtttgccc cgccgccggc ccgatgccgt
     2881 ctgaaaccgt gtcccacaag tccgacagca gccgcaacac gtccgcctcg cggcgcaatg
     2941 tttcgcccaa atgccccttt gggacggctt tcaggcagga tgccgccaag ccgcgcaggt
     3001 tcgggggcaa accccatatc ctgaccggtt cgcggtaaag caggggtgcg aaacgcgcgt
     3061 attccggacg cagcagccat tgttccatac ctttaatggt cattttgacc gttttgccgc
     3121 cgcgataaag atagcggctg aacaggactt cttccccgac tgaaatcagg atgaccttgc
     3181 cgccgtccgc atcgatttgg aatcggctcc ggttgatgcc gaagtccaaa ctgccttcca
     3241 gcaagagcac gaacgacaca taaggttcgg cgaggcggct gctgcaaaaa tcacaccgtg
     3301 ccgttactgt gccgccgtgg atggaaatgc cgttggacaa agtatcgaaa cggtagctgc
     3361 cctccacata gctgccgccg ccgcttttga ctagattgac gaaccggctg ctgttcaaat
     3421 gttctgcttt ttccatagtt ttctgataaa ggttatcatt tgaaagataa catttttcgc
     3481 cacagcaaac aatccgccgc cgacaacggc attttcacaa catttaccgt ccgtatgccg
     3541 tctgaaaatt gcccgtcttc ccgataagcc ttaaaataca cgccgtcaaa cctaccgtcc
     3601 gtccgtacat gagcccatcc ccctttatcg aaatgaaaga cgtcgccttc gcgtatggcg
     3661 accgcccgat tctgaagaac atcaatttca gcattccgca aggcaatttt gctgccgtaa
     3721 tgggcggttc gggcagcggc aaaaccacgc tgatgcgact gattacagga cagattcgtc
     3781 cgcagtccgg gcaggttttg attgaaggac gggatttggc gggcttttcg gctgacgagt
     3841 tgtatgaaca ccgccgccgt atgggcgtat tgttccaaca cggcgcgctg tttactgatt
     3901 tgtcggtatt cgacaatatc gcttttccga tgcgcgaact gacgcgcctg ccggaagcgg
     3961 tgattcggga tttggttttg ttgaaattga acgcggtcgg tctgcgcggt gtggaaaacc
     4021 tgatgccgtc cgagttgtcc ggcgggatgt cgcgccgcgt cgcgcttgcc cgcacgattg
     4081 cgctcgaccc tgaaattatg ttgtacgacg agccgtttac cggcctcgat ccgatttcct
     4141 tgggcgtgat tgcccacttg atcagccgcg tcaacaaggc tttgcgttcg accagtatta
     4201 tggtaacgca cgacattgaa aaatctttgg aaatcgtcga tcaggtgatt ttcttggcgc
     4261 acggcgaaat tatgttctcc ggctcgccgc aggaaatgcg cgaactggat tcgccttggg
     4321 tgcgccagtt tgtcggcggg ctggcagacg gccccgtagc ataccgttat ccggcgcaaa
     4381 cgtcgttgca gcaggacctg ttgggataaa tgagttgatg ccgtctgaaa ccgttgaact
     4441 ttacacatag aaatttatga actttatccg ttccgtcggg gcgaaaaccc tcggccttat
     4501 tcaatccctc ggcagtatca cgctgtttct gctgaacatt ctggcgaagt ccggtacggc
     4561 tttcgtccgt ccgcgcctga gcgtgcgcca agtgtatttc gccggcgtgc tgtcggtgct
     4621 gattgttgcc gtttcggggc tgttcgtcgg catggtcttg ggtttgcagg gctatacgca
     4681 gttgtcgaaa ttcaaatccg ccgatatttt gggctatatg gtcgcggctt cgctattgcg
     4741 cgagctgggt ccggtattgg cagcgatttt gtttgccagc agcgcgggcg gtgcgatgac
     4801 cagcgaaatc ggtctgatga aaacgaccga acagctcgaa gcgatgaatg tgatggcggt
     4861 aaaccctgtc gcccgcgtgg ttgcgccgcg cttttgggcg ggcgtgtttt ccatgccgct
     4921 tttggcttcg attttcaacg tggcgggcat tttcggtgcg tatttggtcg gtgtaagctg
     4981 gctgggcttg gacagcggca ttttctggtc gcaaatgcag aacaacatca cgatacatta
     5041 cgatgtaatc aacggtctga ttaaatccgc cgcgttcggc gtggcggtaa cgctgattgc
     5101 cgtgcatcag ggcttccatt gcgtcccgac ttcggaaggc attttgcgcg ccagcacgcg
     5161 cacggtggtt tcgtccgccc tgacgatttt ggcggtcgat tttatattga ccgcgtggat
     5221 gtttacagat tgacaggccg tctgaagacg aaacgcggaa catcggatat tcaaggaact
     5281 ttaatgaaaa agaacatatt ggaattttgg gtcggactgt ttgtcctgct cggcgtggca
     5341 gcggtcggct ttctcgcttt ccgcgtggca ggcggcgcgg ccttcggcaa ttccggcaag
     5401 acttataccg tatatgccga tttcagcgac atcggcggac tgaaggccaa cgcgccgatc
     5461 aaatccgcag gcgtattggt cggacgcgtt ggttccatcg agcttgaccc gaaatcctat
     5521 caggcgaaag tccgtctcga tttggacagc aaatatcagt tcagtagcga cgtttccgca
     5581 caaatcctga cttcgggact tttgggcgag cagtacatcg ggctgcagca gggcggcgac
     5641 acggaaaacc ttgctgccgg cgacaccatc tccgtaacca gttctgcaat ggttctggaa
     5701 aaccttatcg gcaaattcat gacgagtttt gccgagaaaa atgccgacgg cggcaatgcg
     5761 gaaaaagccg ccgaataaac gtatgccatc atattcaaac taggacactt aatcatgaaa
     5821 aaatcctccc tcatcagcgc attgggcatc ggtattttga gcatcggcat ggcatttgcc
     5881 gcccctgccg acgcggtaag ccaaatccgt caaaacgcca ctcaagtatt gagcatctta
     5941 aaaaacggcg atgccaacac cgcccgccaa aaagccgaag cctatgcgat tccctatttc
     6001 gatttccaac gtatgaccgc attggcggtc ggcaaccctt ggcgcaccgc gtccgacacg
     6061 caaaaacaag cgttggccaa agaatttcaa accctgctga tccgcaccta ttccggcacg
     6121 atgctgaaat taaaaaacgc caacgtcaac gtcaaagaca atcccatcgt caataaaggc
     6181 ggcaaagaaa tcatcgtccg cgccgaagtc ggcgtacccg ggcaaaaacc cgtcaacatg
     6241 gacttcacca cctaccaaag cggcggtaaa taccgtacct acaacgtcgc catcgaaggc
     6301 gcgagcctgg ttaccgtgta ccgcaaccaa ttcggcgaaa ttatcaaagc gaaaggcgtg
     6361 gacggactga ttgccgagtt gaaagccaaa aacggcggca aataaccgac aaaccatacc
     6421 gtccgagggc ggtatgggtt ttccgagcaa acgctatgca tacagaactc aaaaacggaa
     6481 tattgcacat cggcggcgac atcaccgtca aaaccctgac cgccgccgct tttacccgct
     6541 tcagacagca atgtcgtctg aaagacacct gcgcggttga tttgagcggc gtcggacgcg
     6601 ccgattccgc ctgcgtgtcg ctgctgctcg aagtgctgcg cggttgcaaa ggcagcgtca
     6661 ggctgaccgg cattcccgaa tccgtgcgcg cgctgtccga actgtacgaa atcaaagact
     6721 ggctgaaatc atgaaaaaaa ccgcctatgc cttcctcctg ctgatcgggt tcgcttccgc
     6781 ccctgcattt gccgaaaccc gccccgccga cccttatgaa ggctacaacc gcgccgtttt
     6841 caaattcaac gaccaagccg accgctacat tttcgcccct gccgcgcgcg gctaccgcaa
     6901 agttgcgccg aaacccgtcc gcgccggcgt gtccaatttt tttaacaacc tgtgcgacgt
     6961 ggtcagcttc ggtagcaata tcttgcgctt ggacatcaaa cgcgcaagcg aagaccttgt
     7021 ccgcgtcggc atcaacacca ctttcggttt gggcgggctt atcgacatcg ccggcgcggg
     7081 cggcattccc gacaataaaa acaccttggg cgacacgttt gcttcgtggg gatggaaaaa
     7141 cagcaattat ttcgtgttgc ccgtcttagg gccgtccacc gtccgcgacg cgctcggcac
     7201 gggtattacc tccgtttatt cgcccaagaa tatcgtcttc cgcacccctg tcggacgctg
     7261 gggcacgact gccgtatccg ccgtcagtac gcgcgaaggt ctgctcgatt tgaccgacag
     7321 tctggacgaa gccgccatcg acaaatacag ctacacgcgc gacctctata tgaaagtccg
     7381 tgcgcggcag accggtgcaa cacctgccga aggtacggaa gataacatcg acatcgacga
     7441 attggtcgaa agtgccgaaa ccggcgcggc ggaaactgcc gttcaagaag attccgtatc
     7501 cgaaacacag gcagaagcag caggggaagc cgaaacgcaa cctggaacac aaccttaaag
     7561 ccatgccgtc tgaaaatccg tgttcggacg gcattccctt ctgtcggaac ggacagtccg
     7621 aaaccacaga ttgggctata atctcttttt tgcgcatacc gcgcgacagc aaaggaaaaa
     7681 ccatgtacga agtcaaccgc agcgtattcg tcctcatccc tttagaacct ttttggaact
     7741 ggctgcaaac cctgccgggc aaccacctcg acggactgac tcttgaagac atccaagccg
     7801 acgccaattc ctaccttgtc cgcccgtgcg aaaccgccga cgaagtatgg gacgaaatcg
     7861 aagcccgttt tgaagacatt ttcgccgccg aacttgccga ctggtgcgaa gacgaacgcg
     7921 agtggcctgc gctcgatgcc gacattttca acgaatggtt cgacatccag ctttctaccg
     7981 tcatcaccga cctcgaacac gaaccgcttg cccgcgaagc cttccaaccc atcaacctga
     8041 actgatgaag ctgaccgtcc gcaactacca tctcgacggc tacggccatg tcaacaatgc
     8101 gcgctacctc gaatttttcg aagaagcgcg ctgggcgttt tttgaagaac gcggactgct
     8161 gcacgagttg gcaggcctca tactgattgt cgcccgcatc gacatccgat acagccgccc
     8221 cgccgtcgaa ggcgacgtat tgcagttttc atgccgcctg aaaacccccg gcacgcgccg
     8281 tattgtgctg acccagacga ttacactgcc aaacggcaaa accgccgcag aagccgacat
     8341 caccctgatg cccgtccacg ccgccacaca acgcaccgtc agcctgcccg ccacccttgc
     8401 ccgcgcattg gaagccttgt ccgaatgaaa aaaatactca ccgccgccgt cgtcgcactg
     8461 atcggcatcc tccttgccat cgtcctcatc cccgacagca aaaccgcgcc cgccttctcc
     8521 ctgcccgacc tgcacggaaa aaccgtttcc aacgccgacc tgcaaggcaa agtaaccctg
     8581 attaattttt ggtttccctc ctgtccgggt tgtgtgagcg aaatgcccaa aatcattaaa
     8641 acggcaaatt actataaaaa caaaaacttc caagtccttg ccgttgcaca acctatcgat
     8701 ccgatagaaa gcgtccgcca atatgtcaaa gactacggtt tgccgtttac cgtcatgtat
     8761 gatgcggaca aagctgtcgg acaggcgttc ggcacacagg tttatccgac ttccgtcctt
     8821 atcggcaaaa aaggcgaaat cctcaaaact tatgtcggcg aacccgattt cggcaaactc
     8881 taccaagaaa tcgataccgc gctggcacaa tagcgatgcc gtctgaagcg caaaaaaaac
     8941 agaaacgatg ttcctgtttt ttcagatcat ccgccccgac acgttcagac ggcattcaac
     9001 cttccggcag attctttttc accactgccg gattgcctgc cgccaaagag tagggcggaa
     9061 tgtcttttgt aaccaccgcg cctgcgccca cgaccgaacc gcgtccgacg gttacgcccg
     9121 ccattacaat caccctgtgc cccggccaga catcgtcctc caacgtaatc ggacggattt
     9181 ccgtgtagcc ctcaaagcgt tttttgaacg gttaaacttg tggttatttg aataaaacag
     9241 acattccggc tccatcatca catttttgcc gaccaccggc ccacggcaga tttcacagtt
     9301 tgccccgatg cccgagccgt cgcccaaaac cgtatccgga aacacatacg ccccgcgttc
     9361 gatattgacc ccgcgttcga tatgcggaga aacccgccgc gccaaaaaac cgcgcacccg
     9421 tctgccgacg atgccgatgc cgcgcatatg tgacggcggc aaccccgaac cggcaaaacg
     9481 cagcagcagg aaaaagaatt ttgatcccat caaacctccc tcaaaagaac
//
LOCUS       DAXHSF010000064         9455 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.064, whole genome shotgun sequence.
ACCESSION   DAXHSF010000064 DAXHSF010000000
VERSION     DAXHSF010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9455
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.064"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(130..1047)
                     /gene="thrB"
                     /locus_tag="ACFM8S_001132"
     CDS             complement(130..1047)
                     /gene="thrB"
                     /locus_tag="ACFM8S_001132"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFC6314209.1"
                     /translation="MSVYTSVSDDEMRGFLSGYDLGEFVSLQGIAQGITNSNYFLTTT
                     SGRYVLTVFEVLKQEELPFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVA
                     CLKGSDTALPTAEQCFHTGAMLAKMHLAAADFPLEMENPRYDAWWTEACARLLPVLSR
                     DDAALLCSEIDALKDNLGNHLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACWGNFMY
                     DLAIAVNDWARTADNKLDEALKKAFIGGYEGVRPLSAEEKAYFPTAQRAGCIRFWVSR
                     LLDFHFPQAGEMTFIKDPNAFRNLLLSLD"
     gene            complement(1084..1812)
                     /gene="ubiG"
                     /locus_tag="ACFM8S_001133"
     CDS             complement(1084..1812)
                     /gene="ubiG"
                     /locus_tag="ACFM8S_001133"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFC6314210.1"
                     /translation="MGGKMSDKKYNVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLR
                     LDYIDGHADLCGKRVLDVGCGGGILAESMARRGAAFVKGIDMAEQSLETARLHAALNN
                     VADIEYECIRVEDLAEAEPHSFDVVTCMEMMEHVPDPAAIVRACANLVKPDGMVFFST
                     INKNPKSYLHLIVAAEYLLKFVPKGTHDWKKFIAPADLARMCRQAGLDVVETKGMTYH
                     VLSQTYALCDSTDVNYMFACRPAF"
     gene            1964..3205
                     /locus_tag="ACFM8S_001134"
     CDS             1964..3205
                     /locus_tag="ACFM8S_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter"
                     /protein_id="HFC6314211.1"
                     /translation="MPTKTPSLFGGAMIIAGTVIGAGMLANPTATSGVWFTGSLAVLL
                     YTWFSMLSSGLMILEVNTHYPHGASFDTMVKDLLGRGWNIINGIAVAFVLYLLTYAYI
                     FVGGDLTAKGLGSALGGNVSLTVGQLVFFGILAFCVWASARLVDRFTSVLIGGMVLTF
                     IWATGGLIADAKMPVLFDTQAPVGTNYWIYAATALPVCLASFGFHGNVSSLLKYFKGD
                     APKVAKSIWTGTLIALVIYVLWQTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQT
                     GNMDKILSLFSYMAIATSFLGVTLGLFDYIADIFKWNDSVSGRTKTAALTFLPPLISC
                     LLFPTGFVTAIGYVGLAATVWTGIIPAMLLYRSRKKFGAGKTYKVYGGLWLMVWVFLF
                     GIVNIAAQVLSQMELVPVFKG"
     gene            3213..3344
                     /locus_tag="ACFM8S_001135"
     CDS             3213..3344
                     /locus_tag="ACFM8S_001135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314212.1"
                     /translation="MPSEARRRLQTALPQQTATVFRHTAHYPAPHAQIPPRQTGTQP"
     gene            3355..4410
                     /locus_tag="ACFM8S_001136"
     CDS             3355..4410
                     /locus_tag="ACFM8S_001136"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC6314213.1"
                     /translation="MKLKIDIATNNFKHGGGTERYTLDLVKGLNRQNITPAVYATKFD
                     HGIPEYAMIEPHLVDQHRTLKKLRSFLFSSRLAQTRKNSAAKLIACHHADYADLLICG
                     GTHLGYLHHMAQKPNLLDRLAIRRNRSNYATAKLIMAHSHMMRRELVGLYGVPPERIQ
                     VAPPPADTERFFPQPGETADLRAKYGFADYETVFLFPSTGHTRKGLELLADFFEHTGL
                     PVKLAVVGSPLPRPMKNVVGLGFCTDMPELYRAADFTIMASLYEPFGLVGVESVLCGT
                     RVVLSENMACTEVMNEEAGFFFSRQNPETLAQAVAQAVSLKKQGGHRLSDPMRALNYN
                     PSLSHHIDRLTDILASV"
     gene            complement(4477..6129)
                     /locus_tag="ACFM8S_001137"
     CDS             complement(4477..6129)
                     /locus_tag="ACFM8S_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFC6314214.1"
                     /translation="MVAYVFLFLFVTAALVLIIRSHYRWTYFFASALFVFLAGGMLML
                     TAQWQRALNFASVWFVVLILFHRLKIHYYKQPLLISDFLLIADWRNWETLFHYKEAVI
                     GMAGLLALAAYAVFGWSGADALGLPWRWAGTVLFAAAFVSMRHFSKHPGAVKTWLDSL
                     PDDGRDVFLNLPMSCRAVFFQVPVFEGDGEAFAKQMPSETQPYGMSDEKPDIVVTLME
                     STLDPHCFDFAAAKIPDLKMFGRQEDTVFSSPLRVHTFGGATWKSEFAFLAGVPSTDF
                     GALASGVFYSVVPHLQTGFVRNLREHGYFCVALSPFTKGNYNAKAAYDHFGFNLMFQP
                     QDLGYPAPMGKNLWHISSEEMMQYARMILEKRHPDLENVRQPMFVYVLTMKEHGPYRT
                     DTDNVFDLDAPDLNAKTVSALNDYIGRIADLDKAVESFDRYLHERGKPFVFGYFGDHQ
                     VPFEGVSVRKKWDYAQPDYVTQFAVRSNIAGGFVQRQDFLDLAFAGGVLMEAAGLEAK
                     DGFMRANMAMRGLCGGGLEDCPNRKLVGDYRNYLYDVLKIVR"
     gene            6299..6868
                     /locus_tag="ACFM8S_001138"
     CDS             6299..6868
                     /locus_tag="ACFM8S_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFC6314215.1"
                     /translation="MNYKIYDGAKIIKKENLSIGDYSQIDDFVFFNCGEKSILGSFVH
                     ISSFTSIIGGGEFYMDHFSGLSAGCRIITGSDDFMGGGLTNPTVPSKYTNVKKSSVRI
                     GKHAILGTNVTVLPGVTIGDGVAVGAGAIVRKNLEPWTIYVGVDCKPLKRRSQDKILE
                     LEKHLVAEFNLDLCPIIPSPTRPNPKETR"
     gene            6865..7428
                     /gene="gmhB"
                     /locus_tag="ACFM8S_001139"
     CDS             6865..7428
                     /gene="gmhB"
                     /locus_tag="ACFM8S_001139"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFC6314216.1"
                     /translation="MKLIILDRDGVINRDRDDFVKSADEWIPVEGSMDAVAFLTQAGC
                     TVAVATNQSGIGRKYFTVQDLTEMHAKMHRLIRQAGGEIDGIWFCPHTGADGCGCRKP
                     KPGMIEDILERFNAQASETWLVGDSLRDLQAIDAVGGKPALVLTGKGKKTLSQHGHEL
                     PEHTQVFDTLLDFSQYIMQENAAPQAD"
     gene            7458..8201
                     /locus_tag="ACFM8S_001140"
     CDS             7458..8201
                     /locus_tag="ACFM8S_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFC6314217.1"
                     /translation="MLIIRNLIYWLILCSSLIFLFPFMLLASPFQDGAHKMARIWVGI
                     LNWSLKHIVGLKYRIIGAEHIPDRPAVICAKHQSGWETLALQDIFPPQVYVAKRELFK
                     IPFFGWGLKLVKTIGIDRSNRREANEQLIKQGLARKNEGYWITIFPEGTRLAPGKRGK
                     YKLGGARMAKMFEMDIVPVALNSGEFWPKNSFLKYPGEITVVICPTIPYASGSEAELM
                     EKCEHLIETQQPLISGAGPFAAKMPSETA"
     gene            8198..8890
                     /locus_tag="ACFM8S_001141"
     CDS             8198..8890
                     /locus_tag="ACFM8S_001141"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFC6314218.1"
                     /translation="MTAFVHTLSDGMELTVEIKRRAKKNLIIRPAGTHTVRISVPPCF
                     SVSALNRWLYENEAVLRQTLAKTPPPQTAENRLPESILFHGRQLAPTAHQDTQILLMP
                     SEIRVPEGAPEKQLALLRDFLERQAHSYLIPRLERHARTTQLFPASSSLTSAKTFWGV
                     CRKTTDIRLNWRLVGAPEYVADYVCIHELCHLAHPDHSPAFWELTRRFAPYTPEAKQW
                     LKIHGRELFALG"
     gene            8930..9115
                     /locus_tag="ACFM8S_001142"
     CDS             8930..9115
                     /locus_tag="ACFM8S_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314219.1"
                     /translation="MPEQARARPIQTAMTLCRRFGAGWRHTRRLPRFISHRSSETRNP
                     PGPTCGMKRLSWREVFY"
BASE COUNT         2360 a         2922 c         2210 g         1963 t
ORIGIN      
        1 gtcctgattt ttgttaatcc actataaaat ggaaaatact cggctatcgt ctcattttcg
       61 ttttaatcag ccataaaaat gccgtctgaa accctttcag acggcatttc tgtcaaacgc
      121 cggacgcact caatccaaac tcaacagcag gttgcggaac gcgttcgggt ctttgataaa
      181 cgtcatctcg cccgcctgcg gaaaatgaaa gtccaacagg cgcgacaccc aaaaacggat
      241 gcagccggca cgttgggcgg tcgggaaata cgccttttct tcggcactca aggggcgcac
      301 gccctcataa ccgccgataa acgccttttt caacgcctca tccaacttat tgtccgccgt
      361 ccttgcccaa tcgttgaccg caatcgccaa gtcatacata aaattgcccc agcaggcata
      421 atagaaatcg atgaaacccg atacctgacc gccgtcaagc aacacattgt ctttaaacaa
      481 atcggcgtgg atgatgcccg aaggcagatg attgccgaga ttgtccttca gcgcatcgat
      541 ttcggaacac agcagcgcgg catcgtctcg cgacaacacg ggcagcagtc gggcgcacgc
      601 ctccgtccac cacgcatcgt aacgcgggtt ttccatttcc aaagggaaat cggcggcggc
      661 aaggtgcatt ttggccagca tcgcaccggt atggaaacac tgctccgccg tcggcagcgc
      721 ggtatccgaa cctttcaggc aggcaaccaa acaagccggc ttgcccgcca aaacggaatc
      781 aagccggccg tctttgcgcg caaccggcgc ggcaaccgcc acgcccttca tactcaaatg
      841 ccggttaagc tccagaaaaa acggcagctc ttcctgtttc aacacttcaa acacggtcag
      901 cacataacgt cccgaagttg tcgtcagaaa ataattgctg ttggtaatcc cctgcgcgat
      961 gccctgcagg gaaacaaatt cccccaaatc gtaaccgctc aggaagccgc gcatttcatc
     1021 atcggaaaca ctggtataga cagacatatc aactcacttg ctcaaaaacg ggcaaacccg
     1081 ccgtcagaac gccggacggc aggcaaacat ataattcaca tcggtcgaat cgcacagggc
     1141 ataagtttgc gacaacacat ggtaagtcat acccttagtt tccaccacgt ccaaacccgc
     1201 ctgacggcac atccgcgcca aatcggcagg cgcgatgaat tttttccagt cgtgcgtgcc
     1261 tttggggaca aacttcaaca gatattccgc cgccacaatc agatgcaggt acgatttcgg
     1321 gtttttattg atggtggaaa aaaacaccat gccgtccggt ttgaccagat tggcacaagc
     1381 acgcacgatg gcggcgggat cggggacgtg ttccatcatt tccatgcacg ttaccacatc
     1441 gaacgagtgc ggttccgcct cggcaaggtc ttccacgcgg atacattcgt attcgatatc
     1501 ggcgacattg ttcaaagccg cgtgcaggcg ggcggtttcc agcgactgct ccgccatgtc
     1561 gatgcccttt acaaacgccg cgccgcgccg cgccatactt tccgccaaga tgccgccgcc
     1621 gcagcccacg tccaaaaccc gtttgccgca caaatccgcg tgtccgtcga tataatccag
     1681 ccgcagcgga ttgatgtcgt gcaaggtttt gaactcgccc gacttgtccc accatttgtc
     1741 ggcaatccgg ctgaatttgg cgatttcccc ctcatcgaca ttatattttt tgtcggacat
     1801 tttccctccc atctgacgaa ccgcccactc caaaacccaa gattatacta tggaacacaa
     1861 tgccgtctga aagccttttc gggcggcgcg gcgcaaatgc cttacaaatc cttacacttt
     1921 acggcataat ggcggctcgc tttttctggc agaaagacaa aatatgccca ccaaaacccc
     1981 ttcactgttc ggcggcgcga tgattatcgc cggcacggtc atcggcgcag gtatgctcgc
     2041 caacccgacc gccacatccg gcgtatggtt taccggctcg ctggccgtgt tgctgtacac
     2101 ctggttttct atgctttcca gcggcctgat gattttggaa gtcaacaccc attatccgca
     2161 cggcgcaagt ttcgacacga tggtcaaaga cctgctcgga cgcggctgga acatcatcaa
     2221 cggcatcgcc gtcgccttcg ttttatacct gctcacctac gcctacatct tcgttggcgg
     2281 cgacctgacc gccaaaggct taggcagcgc cttgggcggc aatgtttcac tcaccgtcgg
     2341 acaactcgtc ttcttcggca tcctcgcctt ttgcgtatgg gcatccgcac gcttggtcga
     2401 ccgcttcacc agcgtcctca tcggcggcat ggtattaacc tttatttggg caaccggcgg
     2461 cctgattgcc gatgccaaaa tgcccgtcct gttcgacacc caagcccccg tcggcaccaa
     2521 ctactggatt tacgccgcca ccgccctgcc cgtctgcctt gcgtcattcg gtttccacgg
     2581 caacgtctcc agcctgctca aatactttaa aggcgacgcg cccaaagtgg ctaaatccat
     2641 ctggacgggc acactgattg cgctggtaat ttacgtcctc tggcaaaccg ccatccaagg
     2701 caacctgccg cgcaacgagt tcgcccccgt gattgccgcc gaagggcaag tctccgtcct
     2761 catcgaaacc ctgtccaaat tcgcccaaac cggcaatatg gataaaatat tgtccctatt
     2821 ttcctacatg gcgatcgcca cctcgttttt aggtgtaacg ctcggactct tcgactacat
     2881 cgccgacatc ttcaaatgga acgacagcgt gtccggccgc accaaaaccg ccgcgctgac
     2941 cttcctgccg cctctaattt cctgcctgct cttccccacc ggctttgtta ccgccatcgg
     3001 ctacgtcggc ctggcggcaa ccgtctggac aggcatcatc cccgccatgc tgctctaccg
     3061 ttcgcgcaaa aaattcggcg caggcaaaac ctataaagtt tacggcggct tgtggctgat
     3121 ggtttgggtc ttccttttcg gcatcgtcaa catcgccgca caggtattga gccaaatgga
     3181 actcgtcccc gtatttaaag gataaaggca aaatgccgtc tgaagcccgc cggcggcttc
     3241 agacggcatt gccgcaacaa acggcaaccg tattccggca cacagcgcat taccctgccc
     3301 ctcacgcaca aatcccgccc cgacaaaccg ggacgcaacc ataaaggaac aatgatgaag
     3361 ctcaaaatag acattgcaac caacaacttc aaacacggcg gcggcacgga acgctacaca
     3421 ttggatttgg taaagggtct gaacagacaa aacatcacac cggccgttta tgcgacgaaa
     3481 tttgatcacg gcattcctga atacgccatg atcgaacccc atcttgtcga tcaacaccgg
     3541 acgctgaaaa aactacgctc attcctcttt tcaagccggc tcgctcaaac cagaaaaaac
     3601 agtgccgcca aactgattgc ctgccaccac gccgattacg ccgacctcct catctgcggc
     3661 ggcacacact tgggctacct gcaccatatg gcgcaaaaac cgaacctgct cgaccgcctc
     3721 gccatacgcc gtaaccgcag caactacgcc accgccaaac tgattatggc gcattcccat
     3781 atgatgcggc gcgaactggt cggactgtac ggcgttcccc ctgaaagaat ccaagtcgcc
     3841 cccccccccg cagatacgga acgcttcttc ccgcaacccg gagaaactgc cgacctgcgc
     3901 gccaaatacg gctttgccga ctatgaaacc gttttcctgt tcccatcgac cggccacacg
     3961 cgcaaaggtc tggaactgct tgccgacttt ttcgaacata ccggcctgcc cgtcaagctc
     4021 gccgttgtcg gctccccgct cccccgccct atgaaaaacg tcgtcgggct gggcttctgc
     4081 accgatatgc ccgaactcta ccgcgccgcc gactttacca ttatggcctc cctgtacgaa
     4141 cccttcgggc tggtcggtgt cgaatccgtc ctatgcggca cacgcgtcgt cctctccgaa
     4201 aacatggcat gtacagaggt catgaacgaa gaagccggat tctttttctc acgccaaaac
     4261 ccggaaaccc tggcgcaagc cgttgcccaa gccgtcagcc ttaaaaaaca gggcggacac
     4321 cgcctgtccg acccgatgcg tgcactgaac tacaacccgt ccctgtcgca ccacatcgac
     4381 cgactgaccg acatactggc atccgtataa caaaatgccg tctgaacgag ttttcagacg
     4441 gcatttttgc aaccgttgca gccgtgcggc aaaaagctaa cggacaattt tcaaaacgtc
     4501 gtacagatag ttccgatagt ctccgaccag tttccggttc gggcagtctt ccaaccctcc
     4561 gccgcacaaa ccgcgcatcg ccatattcgc acgcatgaag ccgtctttgg cttccagacc
     4621 agccgcctcc atcagcacgc cgcctgcaaa ggcaaggtcg aggaaatcct gccgctgtac
     4681 gaatccgccg gcaatattgc tcctgacggc aaactgcgtt acataatccg gctgcgcgta
     4741 atcccatttc ttcctgacgg acacgccctc aaacggtacc tgatgatcgc cgaaataacc
     4801 gaaaacaaag ggtttgccgc gttcgtgcag ataacggtcg aaactttcca ccgctttgtc
     4861 caaatcggca atgcgcccga tgtagtcgtt aagcgcggat acggttttcg cattcaaatc
     4921 aggtgcatcc aaatcaaaca cattgtcggt atccgtccga tacggcccgt gctccttcat
     4981 ggtcagcaca tatacgaaca tcggctgccg cacgttttcc aaatcgggat ggcgtttttc
     5041 caaaatcatc cgcgcatact gcatcatttc ctcactggaa atatgccaca ggtttttgcc
     5101 catcggcgcg ggatagccca aatcttgcgg ctggaacatc agattaaagc cgaaatggtc
     5161 atatgctgcc ttggcattgt agttgccctt ggtaaacggc gagagcgcca cgcaaaaata
     5221 accgtgttcg cgcaggttgc ggacaaaacc ggtctgcaaa tgcggcacga ccgagtaaaa
     5281 cacgccgctt gccaacgcgc cgaaatccgt ggacggaaca cccgccaaaa acgcaaattc
     5341 tgacttccac gttgcgccgc cgaaagtatg cacgcgcaaa ggtgaggaaa atacggtatc
     5401 ttcctgccgt ccgaacattt tcaaatcggg aatcttggcg gcggcaaagt cgaaacagtg
     5461 cggatcgagc gtcgattcca tcagggtaac gacaatatcg ggtttctcat ccgacatgcc
     5521 gtacggctgg gtttcagacg gcatctgttt ggcaaacgct tccccgtcgc cctcgaacac
     5581 gggtacttgg aaaaacaccg cccgacagga catcggcagg ttcaaaaata cgtcgcgccc
     5641 gtcgtccggc agcgagtcca gccacgtctt taccgcgccc ggatgtttgg aaaaatgccg
     5701 catcgacacg aacgccgccg caaacaaaac cgtgcctgcc caacgccatg gcaaacccaa
     5761 agcatccgca ccgctccagc cgaaaaccgc atatgccgcc aaagccagca gccccgccat
     5821 accgataacc gcttccttat aatggaacag cgtttcccaa ttccgccagt cggcaatcag
     5881 caaaaagtcg gaaatcaaca acggctgttt gtaataatgg attttcagcc tgtggaacag
     5941 tatcagcacc acaaaccaga ccgaagcgaa attcaaggcg cgctgccact gagccgtcaa
     6001 catcagcata ccgcccgcca aaaagacaaa cagcgcggat gcgaaaaaat acgtccaacg
     6061 gtagtgcgac ctgataatca gcaccaacgc cgccgttaca aacaaaaata ggaaaacata
     6121 ggcaaccatc ccgccccact cctcaaaacg gcatacctga gccgttgcga attattatcg
     6181 aaagcgtgca ttataatgca gcacaggcgc atcactgccg atatttcagt ataattattc
     6241 gagatggaat cactgaaaat ggtgaagtta caatgagccc ttttagagga actaaaaagt
     6301 gaattataaa atatacgatg gtgcaaagat tattaaaaaa gaaaatttat caattgggga
     6361 ttattctcaa attgatgatt ttgtattttt taattgtgga gagaaaagta ttttaggaag
     6421 ttttgttcat atatcatcat ttaccagtat tattggtggt ggagaatttt atatggatca
     6481 tttttcagga ttaagcgcag ggtgtagaat tattaccggt agcgatgatt ttatgggggg
     6541 ggggttaacc aatccaacag tcccatctaa atatactaat gttaaaaaat ctagtgttag
     6601 aattggaaaa catgcaattt taggaacaaa tgtaacagta ttaccaggcg ttactattgg
     6661 tgatggggtt gctgttggag caggggctat agtgagaaaa aatcttgagc cgtggactat
     6721 ttatgtcggg gtagattgta aaccactgaa gcgcagatca caagataaaa tattggagtt
     6781 agagaaacat cttgtagcag aatttaatct agatttatgt cctataatcc catccccgac
     6841 acgccccaac ccaaaggaaa cgcgatgaaa ctcattattc tcgaccgcga cggcgtcatc
     6901 aaccgggacc gcgacgactt cgtcaaatcc gccgacgagt ggatacctgt cgaaggcagt
     6961 atggatgcgg tggcattcct gacgcaggcg ggctgcaccg ttgccgtcgc caccaaccaa
     7021 tccggtatcg ggcgcaaata ttttaccgtt caagacctta ccgaaatgca cgccaaaatg
     7081 caccgcctca tccgtcaggc aggcggggaa atcgacggca tctggttctg cccgcatacc
     7141 ggcgccgacg gctgcggctg ccgcaagccc aaaccgggca tgattgaaga catcctcgaa
     7201 cgcttcaacg cccaagcttc ggaaacttgg ctggtcggcg acagcctgcg cgatttgcag
     7261 gcaatcgatg ccgtcggcgg aaaacccgcg ctggttctga ccggaaaagg caaaaaaacg
     7321 ctctcccaac acggacacga attgcccgaa cacacacagg ttttcgatac cctgctcgat
     7381 ttctcacaat acatcatgca ggaaaacgcc gcaccgcaag ccgactgaac ataccgcatt
     7441 tccgacaagg caaaaccatg ctcatcatcc gcaacctgat ttactggctg atactctgtt
     7501 ccagcctgat tttcctcttt ccctttatgc tgctcgcctc gcctttccag gacggggcgc
     7561 acaagatggc gcggatctgg gtcggcatcc tcaactggtc gctcaaacac atcgtcgggc
     7621 tcaaataccg catcatcggc gcggaacaca ttccggaccg ccccgccgtc atctgcgcca
     7681 aacaccaaag cggctgggaa acgcttgccc ttcaagacat ttttccgccg caggtttacg
     7741 ttgccaaacg cgagctgttt aaaatcccct ttttcggctg gggtttaaaa ctggtcaaaa
     7801 ccataggcat agaccgcagc aaccgccgcg aagccaacga acagctcata aagcagggtt
     7861 tggcgcgcaa aaacgaaggc tattggatta ccattttccc cgaaggcacg cgccttgcac
     7921 ccggaaagcg cggcaaatac aaactcggcg gcgcgcgcat ggcgaaaatg tttgagatgg
     7981 acatcgtccc cgtcgccctc aacagcggcg aattttggcc gaaaaactcc tttctgaaat
     8041 atccggggga aatcaccgtc gtcatctgtc cgaccatccc gtacgcaagc ggcagcgaag
     8101 ccgaattgat ggaaaaatgc gaacacctca tcgaaacgca gcagccgctc atttctggcg
     8161 caggcccgtt tgccgccaaa atgccgtctg aaaccgcatg accgcctttg tccacaccct
     8221 ttcagacggc atggaactga ccgtcgaaat caagcgccgt gccaagaaaa acctgattat
     8281 ccgccccgcc ggcacacata ccgtccgcat cagcgtccca ccctgcttct ccgtctccgc
     8341 tctaaaccgc tggctgtatg aaaacgaagc cgtcctgcgg caaacactgg cgaaaacacc
     8401 gccgccgcaa actgccgaaa accggctgcc cgaatccatc ctcttccacg gcagacagct
     8461 tgcccctacc gcccatcaag acacgcaaat cctgctgatg ccgtctgaaa tccgtgttcc
     8521 cgaaggcgca cccgaaaaac agcttgcgct gctgcgggac tttttggaac ggcaggcgca
     8581 cagttacctg attccccgcc tcgaacgcca cgcccgcacc acacaactgt tccccgcctc
     8641 ctcctcgctg acctctgcca aaaccttctg gggcgtgtgc cgcaaaacca cagacatacg
     8701 cctcaactgg cggctggtcg gcgcaccgga atacgttgcc gattatgtct gcatacacga
     8761 actctgccac ctcgcccatc ccgaccacag ccccgccttt tgggaactga cccgccgctt
     8821 cgcaccctac acgcccgaag cgaaacagtg gctcaaaatc cacggcaggg aacttttcgc
     8881 cttgggctga cacggtcccg ggccggcccg ccgctttcag acggcatccg tgccggaaca
     8941 ggcacgcgcc cgcccgattc aaaccgcgat gacgctttgc cgccggttcg gggcaggatg
     9001 gcggcacacg cgccgtctgc cgcgtttcat ttcacaccgc tcttccgaaa cccgaaaccc
     9061 gcccggtccg acgtgcggta tgaaacgctt aagctggcgc gaagtctttt actgatttgc
     9121 ccgcgaaaat gccgtctgaa aggttttcgg acggcatttt tttgcgtttc ccaggatggc
     9181 ggcggattcg taaaaggcgg tcagggtgga ttgtaggatg ggttgagacc tgccgaatcc
     9241 gccgcatctg ccaaatctac cgccgtcatt cctacgaaag tgggaatcta gaacgcgggg
     9301 ttaagaaaac ctgcaccgcg tcattcccac gaaagtgaga atccaggacg caaaatctcc
     9361 agaaaccgtt ttatccgata agtttccgca ccgacaaaac tagattcccg cctgcacggg
     9421 aatgacgaat ccatccatac ggaaacctgc atccc
//
LOCUS       DAXHSF010000065         9408 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.065, whole genome shotgun sequence.
ACCESSION   DAXHSF010000065 DAXHSF010000000
VERSION     DAXHSF010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9408
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.065"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            202..1020
                     /gene="opc"
                     /locus_tag="ACFM8S_001143"
                     /gene_synonym="opcA"
     CDS             202..1020
                     /gene="opc"
                     /locus_tag="ACFM8S_001143"
                     /gene_synonym="opcA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Opc"
                     /protein_id="HFC6314220.1"
                     /translation="MKKTVFTCAMIALTGTAAAAQELQTANEFTVHTDLSSISSTRAF
                     LKEKHKAAKHIGVRADIPFDANQGIRLEAGFGRSKKNIINLETDENKLGKTKNVKLPT
                     GVPENRIDLYTGYTYTQTLSDSLNFRVGAGLGFESSKDSIKTTKHTLHSSRQSWLAKV
                     HADLLSQLGNGWYINPWSEVKFDLNSRYKLNTGVTNLKKDINQKTNGWGFGLGANIGK
                     KLGESASIEAGPFYKQRTYKESGEFSVTTKSGDVSLTIPKTSIREYGLRVGIKF"
     gene            1183..1827
                     /locus_tag="ACFM8S_001144"
     CDS             1183..1827
                     /locus_tag="ACFM8S_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFC6314221.1"
                     /translation="MLASAIDFILHIDQHLLALSAQYGVWIYAILFLIVFCETGLIVT
                     PLLPGDSLLFAAGGIAALGGMDIHLMVALLSLAAILGDALNFTVGKYFGGRLFANPDS
                     KIFRREYLDKTHRFYEKHGGKTIIIARFMPIVRTFAPFVAGMGKMHYAKFIRYNIIGG
                     LLWVILFSYAGYFFANFPVVKNNLGLVMGGIIIVSVLPGAVEIARAKLAVKSKR"
     gene            1846..3756
                     /locus_tag="ACFM8S_001145"
     CDS             1846..3756
                     /locus_tag="ACFM8S_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC6314222.1"
                     /translation="MNILSVENASFAVGHVALLDKTSFQLDRGEKIGLIGRNGAGKSS
                     FLKILAGVQKLDDGQIIVQNNLKIVYVPQESFFDKEATVFDTVAEGLGEIRDLLRRYH
                     HVSHELENGSSEALLKELNELQLEIEAKDGWKLDAAVKQTLGELGLPENEKIGNLSGG
                     QKKRVALAQAWVQKPDVLLLDEPTNHLDIDAIIWLENLLKAFEGSLVVITHDRRFLDN
                     IATRIVELDRGILRSYPGSFSKYSEKKAQELAVEAEHNRLFDKFHAQEEAWIRKGIEA
                     RRTRNEGRVRRLEELRRQRAERRNVQGQVNFKLDSGEKSGKIIAELEHASFAYGGKVI
                     MDKFSAILQRGDKIGLIGPNGIGKTTFLKLILGELQPTYGRIRIGSKQEVAYFDQFRS
                     ALNENDTVFYTLGQGNDYVEVGGKKKHVMSYLEDFLFPPARAQSPVSSLSGGERNRLL
                     LAKLFTRPANILILDEPTNDLDIDTQELLEDLLRDYQGTVFLVSHDRMFLDNVITQSI
                     IFEGNGRLKEYIGGYQDYIDAKSREAKIQTESAPKTADAEPVKEKPKANRTVKLSYKE
                     QRELDALPDEIAALEAEQAEINAQLSDPEIFKDYEKAGALQSRAEEIEMLLLEKLERW
                     ELLETKQNGNAV"
     gene            3777..4142
                     /locus_tag="ACFM8S_001146"
     CDS             3777..4142
                     /locus_tag="ACFM8S_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314223.1"
                     /translation="MFKRPEEIIVLILAVLWIAGTYFLAALFGADAYTVLKITALTLL
                     WSAASFLLWQKNLSRHIWPLLLGCLTACWWPYLNQSVAPGSLPWYASWTFKTILALIP
                     VTAGYLFKWKQYRQRKTKP"
     gene            4253..5728
                     /gene="trpE"
                     /locus_tag="ACFM8S_001147"
     CDS             4253..5728
                     /gene="trpE"
                     /locus_tag="ACFM8S_001147"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFC6314224.1"
                     /translation="MISKQEYQAQAAQGYNRIPLVQELLADLDTPLSLYLKLANRPYT
                     YLLESVVGGERFGRYSFIGLPCSHYLKASGKHVDVYQNGEIVEQHDGNPLPFIEAFHN
                     RFKTPEIPSLPRFTGGLVGYFGYETIYNFEHFAHRLKNTTKADPLGTPDILLMLSQEL
                     AVVDNLSGKIYLIVYADPSQPDGYERARERLEDIRTQLRQSCAIPLSLGSKHTEAVSE
                     FGEEPFKACVNKIKDYIFAGDCMQVVPSQRMSMEFTDSSLALYRALRTLNPSPYLFYY
                     DFGDFHIVGSSPEILVRRERDDVIVRPIAGTRLRGKTPAEDLANEQDLLSDAKEIAEH
                     VMLIDLGRNDVGRISKTGEVKVTDKMVIEKYSHVMHIVSNVEGRLKDGMTNMDILAAT
                     FPAGTLSGAPKVRAMEIIEEVEPSKRGIYGGAVGVWGFNNDMDLAIAIRTAVVKNNTL
                     YVQSGAGVVADSDPASEWQETQNKARAVIHAAQMVQEGLDK"
     gene            5725..6270
                     /locus_tag="ACFM8S_001148"
     CDS             5725..6270
                     /locus_tag="ACFM8S_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFC6314225.1"
                     /translation="MTYEKLYELRQTLRPTTKDGLYTDNEKSREILTVRWSGNTENPK
                     NFSAVAIGINPSKANDERSDKTLTQLARFLDMYGFTNFKMLNIFSSYSTQQTGIRANT
                     QTDFSKFKGCLEDADMIILAWGTDRGAYKDEKNRILEFLKAEKFMEKVFCISETGNSS
                     DTRHPSRISYSYQLVQFEESA"
     gene            6267..7403
                     /locus_tag="ACFM8S_001149"
     CDS             6267..7403
                     /locus_tag="ACFM8S_001149"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFC6314226.1"
                     /translation="MKNISLSPPAMLGILGGGQLGRMFTVAAKTMGYKVTVLDPDPDA
                     PAAEFADCHLCAPFNDQAALDELAKCAAVTTEFENVNADAMRFLAKHTNVSPGSDCVA
                     IAQNRIQEKAWIRKAGLQTAPYQAICKAEDITEESIQFLPGILKTATLGYDGKGQIRV
                     KTVDELKAAFAEHRGVDCVLEKMVDLRGEISVIVCRLNNDNVQTFDPAENIHENGILA
                     YSIVPARLSADIQQQARQMAQRLADELNYVGVLAVEMFVVGDTHELVVNEIAPRPHNS
                     GHHTVDACAADQFQQQVRLMCNLPPADTKLLSSCCMANILGDVWQEDGGEPDWFPLQS
                     RPDAHLHLYGKKTAHKGRKMGHFTVLTTDSDTAFQEAKKLHQSL"
     gene            7450..7932
                     /locus_tag="ACFM8S_001150"
     CDS             7450..7932
                     /locus_tag="ACFM8S_001150"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC6314227.1"
                     /translation="MNLLTLLRPATVQDCKDIFKVHLHSVQYTCILSYNEHALKVWEG
                     LLNTESYLPTISDPDKALWVAEYKGNIQGFFQIDCQEAQLDALYVHPLFHNLGLGTAL
                     LHQAETIAHKSGLSFLKLYASLNSVPFYLLNRYESLGSAVLQLDPSIKIKCELMRKHL
                     "
     gene            8038..9093
                     /locus_tag="ACFM8S_001151"
     CDS             8038..9093
                     /locus_tag="ACFM8S_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6314228.1"
                     /translation="MKTYAPALYTAALLTACSPAADSNHPSGQNAPANTESDGKNITL
                     LNASYDVARDFYKEYNPLFIKTYQSEHPGTSVSIQQSHGGSSKQALSVANGLQADVVT
                     MNQSSDIDLLEKKGLVEKGWQQALPDHAAPYTSTMVFLVRKNNPKQIRDWNDLAKDGV
                     NIVIANPKTSGNGRYAFLGAYGYGLKTTNGNEQEAQKLVASILKNTPVFENGGRAATT
                     TFTQRNIGDVLITFENEANYVSKKLTQGQFEIVYPSYTISAESPVAVVNSVVAKKGTQ
                     KTARAYLEYLWSEPAQELAASLYLRPRNPEVLARHKADFPDLDSFSPEEKFGGWDNIM
                     KTYFADGGIFDRLTAQK"
     gene            9090..9302
                     /locus_tag="ACFM8S_001152"
     CDS             9090..9302
                     /locus_tag="ACFM8S_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314229.1"
                     /translation="MTAMPSEPPFRRHSTHQNRKTAPKIIITRHNGKPDTKPNTAAKM
                     PSEAVGATILIWGALQGRITFLNHPF"
BASE COUNT         2625 a         2660 c         2062 g         2061 t
ORIGIN      
        1 aaatttcgtc attcccataa aaaacagaaa accaagtgag aataacaatt cgttgtaaac
       61 aaataactat ttgttaattt ttattaatat atgtaaaatc tccccccccc ccgaaagctt
      121 aagaatataa ttgtaagcgt aacgattatt tacgttatgt taccatatcc gactacaatc
      181 caaattttgg agattttaac tatgaaaaaa acagttttta catgtgccat gattgccctg
      241 accggtactg ccgccgctgc acaagagctt caaaccgcta atgagtttac cgtccacacc
      301 gacctctctt ccatttcttc aactcgtgct ttcctgaaag aaaaacacaa agctgccaaa
      361 cacatcggcg tacgtgctga tattcctttt gatgccaacc aaggcatccg cttggaagcc
      421 ggtttcgggc gcagcaaaaa aaatattatt aatttggaaa cagatgagaa caagctgggt
      481 aagactaaaa atgtaaaact gcccaccggc gttcctgaaa accgtatcga tctttacaca
      541 ggctacacct acacccaaac gttaagtgat tctttaaatt tccgtgtggg tgccggcttg
      601 ggttttgaat cttcaaaaga cagcattaaa accaccaagc atacgcttca cagcagccgt
      661 cagtcgtggt tagccaaagt tcacgcggat ttgctttccc aactgggtaa cggctggtat
      721 atcaaccctt ggtctgaagt gaaatttgac ctcaattccc gctataaatt aaacaccggc
      781 gttaccaatc tcaaaaaaga catcaatcaa aaaaccaacg gctggggctt tggattgggt
      841 gcaaatattg gtaaaaaact gggcgaatcc gccagcatcg aggcggggcc gttctacaaa
      901 caacgcactt acaaagaatc cggcgagttt agtgtaacaa ccaagagtgg cgacgtatcg
      961 ctcaccatcc cgaaaaccag tattcgtgaa tacggcttgc gcgtcggcat aaaattctga
     1021 tgatttgaaa tcatcattgt cactttaaat gccaaaccgc aaactatttt ggtttgcggt
     1081 ttttacgtga aatgaatttg aatagccgat gccgtctgaa aatttcagac ggcatttttc
     1141 caatcggtta aaatacgcgg tttgtttttt taggaacgcg ccgtgcttgc ctccgccatc
     1201 gacttcatcc tccatatcga ccaacacctg ctcgcgctgt cggcgcaata cggtgtgtgg
     1261 atttatgcga ttctgttttt gattgttttt tgcgaaaccg gcctgattgt tacgccctta
     1321 ctgcccggcg attcgctgct gtttgccgcc ggcggtattg ccgcgctggg cggtatggat
     1381 attcatttga tggttgcatt attaagcctt gccgccatac tcggcgacgc gctcaatttt
     1441 accgtcggca aatatttcgg cggcaggctg ttcgccaatc ccgattctaa aatcttccga
     1501 cgtgaatatc tcgataaaac ccaccgtttt tacgaaaaac acggcggcaa aaccatcatc
     1561 attgcccgtt ttatgcccat cgtccgcacc ttcgccccct tcgtcgccgg tatggggaaa
     1621 atgcactatg ccaaatttat ccgctacaac atcatcggtg gcttgttgtg ggttatcctg
     1681 ttttcttacg cgggctattt ctttgccaac ttccccgtcg taaaaaacaa tctgggcttg
     1741 gtgatgggcg gcatcattat tgtttccgtc ctgccgggtg cggttgaaat tgcccgcgcc
     1801 aaacttgcgg taaaatccaa acggtaactg ttgagaattt gtatcatgaa tatattatct
     1861 gtagaaaacg cttcctttgc cgtcggccac gtcgccctgc tcgacaaaac ttccttccaa
     1921 ctcgacagag gcgaaaaaat cggcttaatc ggtcgtaatg gtgcgggtaa gtcttcgttt
     1981 ttaaaaatcc tcgccggcgt gcaaaagctt gatgatgggc agattattgt ccaaaacaac
     2041 ctcaaaatcg tttatgtacc gcaggaatcc ttttttgata aggaagcaac cgtatttgat
     2101 actgttgccg aaggtttggg cgaaattcgc gatttattgc gccgttatca tcatgtcagc
     2161 catgagttgg aaaatggttc gagtgaggcc ttattgaaag agctcaacga attgcaactt
     2221 gagatcgaag cgaaggacgg ctggaagttg gatgcggcgg tgaagcagac tttgggcgaa
     2281 ctcggtttgc cggaaaacga aaaaatcggc aacctctccg gcggtcagaa aaagcgcgtc
     2341 gccttggcgc aggcttgggt gcagaagccc gacgtattgc tgctcgatga accgaccaac
     2401 catttggaca tcgacgcgat tatttggttg gaaaacctgc tcaaagcgtt tgaaggcagc
     2461 ttggtcgtga ttacccacga ccgccgtttt ttggataata tcgccacgcg gattgtcgaa
     2521 cttgaccgcg gcattctacg ttcctatccc ggctcgttct ctaaatacag tgagaaaaaa
     2581 gcgcaagagt tggcagtcga ggcggaacat aaccgcttgt ttgacaaatt tcatgctcag
     2641 gaagaagcgt ggatacgcaa aggcattgaa gcgcgccgta cccgtaacga aggccgcgtg
     2701 cgccgcttgg aagagctgcg ccgccaacgt gcagaacgcc gcaacgtgca gggacaggtc
     2761 aactttaagc tcgacagcgg tgagaagagc ggcaaaatca tcgccgaatt ggagcacgct
     2821 tcgtttgcct acggcggcaa agtcattatg gacaaattct ccgccatctt gcagcgcggc
     2881 gacaaaatcg gcttaatcgg tccaaacggc atcggcaaaa ccacattttt aaaactgatt
     2941 ttgggcgaat tgcagccgac ctacggcaga atccgtatcg gcagcaagca ggaagtcgcc
     3001 tatttcgacc agttccgcag cgcgttgaat gaaaacgaca ccgtgtttta cacgctcgga
     3061 cagggcaatg attacgttga agtcggcggt aagaaaaagc atgtgatgag ctatttggaa
     3121 gatttcctgt tcccccctgc ccgcgcgcaa agtcccgttt catcgctctc cggcggcgaa
     3181 cgcaaccgcc ttttgctggc aaaactcttt acccgtcctg ctaatatttt gattttggac
     3241 gaaccgacca acgacttgga tatcgatacc caagagctgc ttgaagattt gctgcgcgat
     3301 tatcaaggca cggtattcct tgtctcacac gaccgtatgt tcctcgataa cgtgattacc
     3361 caaagcatta tttttgaagg aaatggaagg ttaaaagaat atatcggcgg ctatcaggac
     3421 tacatcgacg caaaatcacg cgaagcgaag attcagacgg aatccgcacc caaaacagcc
     3481 gatgccgaac cggtgaaaga aaaacccaaa gccaaccgca cggtcaaact gtcctacaaa
     3541 gaacagcgcg aactcgacgc cctgcccgac gaaatcgccg ccttggaagc cgaacaggca
     3601 gaaatcaacg cgcagctttc cgatcccgaa atcttcaaag attacgaaaa agccggcgca
     3661 ctgcaaagcc gcgccgaaga aatcgaaatg ctgcttttgg aaaagctgga acgctgggag
     3721 ctgctggaaa ccaaacagaa cggcaatgcc gtctgaaatc aaacaggaag aaaaaaatgt
     3781 tcaaacgacc tgaagaaatc attgtcctga tacttgccgt cctctggatt gccggtacgt
     3841 attttctcgc cgccctgttt ggagcggatg cttataccgt gttaaaaatc actgccctta
     3901 ccctgctttg gtctgccgca tcattcctcc tttggcaaaa aaacctcagc cggcatatct
     3961 ggccgctgct gctcggctgc ctgactgcct gttggtggcc gtatctgaat caatcggtcg
     4021 cacccggttc tttaccttgg tatgcatcat ggacgttcaa aaccatctta gccctgattc
     4081 ccgtaaccgc aggttatctg tttaaatgga aacaataccg gcagcgcaaa acaaagccgt
     4141 aagcccgtcc ttttcagacg gcatctccgg tcagactgtc aacaattccg ataaaatgct
     4201 atactcgcgc ccgttgccgc gccgtccgaa accgcccttt gggaaagcag aaatgatcag
     4261 caaacaagaa taccaagccc aagccgctca aggctacaac cgcatcccgc tcgtgcaaga
     4321 actccttgcc gatttggata cgccgctttc cctctatctc aaactcgcca accgccccta
     4381 tacctacctg ctcgaatccg ttgtcggtgg cgaacgtttc ggccgctatt cttttatcgg
     4441 cctgccttgc agccactatc tcaaagccag cggcaaacac gtcgatgttt atcaaaacgg
     4501 cgaaattgtc gagcaacacg acggcaaccc cctgcccttt atcgaagcct tccacaaccg
     4561 cttcaaaaca cccgaaatcc caagcctgcc gcgctttacc ggcggactgg tcggctactt
     4621 cggttacgaa accatctaca attttgaaca cttcgcccac cgcctgaaaa acaccaccaa
     4681 agccgacccg ctcggcacgc ccgacatcct cctgatgctc tcgcaagagc ttgcagtagt
     4741 ggataaccta agcggtaaaa tctacctgat tgtttacgcc gatccgtcgc agcccgacgg
     4801 ctacgaacgc gcacgcgaac gcctcgaaga catccgtaca cagttacgcc aaagttgcgc
     4861 catcccgctt tcgctcggca gcaaacacac cgaagccgtc agcgaattcg gcgaagagcc
     4921 gttcaaagcc tgcgttaaca aaatcaaaga ctacatcttt gcaggcgact gcatgcaggt
     4981 tgtccccagc cagcgcatga gcatggaatt caccgacagc tcgctcgccc tctaccgcgc
     5041 cttgcgcacg ctcaaccctt cgccttatct cttctactac gatttcggcg atttccacat
     5101 cgtcggctcc tcgcccgaaa tcctcgtccg ccgcgaacgc gacgatgtca tcgtccgccc
     5161 catcgccggc acgcgcctgc gcggcaaaac ccccgccgaa gaccttgcca acgaacaaga
     5221 tttgttaagc gatgccaaag aaatcgccga acacgtcatg ttgattgact taggacgcaa
     5281 cgacgtcggc cgcatcagta aaaccggcga agtcaaagtt accgacaaaa tggtgattga
     5341 aaaatactcc catgtaatgc acatcgtttc caacgtcgaa ggccgtctga aagacggcat
     5401 gaccaacatg gacatcctcg ccgctacctt ccctgccggc acactctccg gcgcacccaa
     5461 agtccgcgcc atggaaatca tcgaagaagt cgaaccaagc aaacgcggca tctacggcgg
     5521 tgccgtcggc gtatggggtt tcaacaacga catggatttg gcaatcgcca tccgcaccgc
     5581 cgtagtaaaa aacaacacgc tatacgtcca aagcggcgcg ggcgtggttg ccgactccga
     5641 tccggcctcc gaatggcagg aaacacaaaa caaagcccga gccgtcatac acgccgcgca
     5701 gatggtgcag gaagggttgg ataaatgact tatgaaaaac tttatgagct tcgccaaaca
     5761 ctcagaccga ctaccaaaga cggtctctat acagataatg aaaaaagtcg agaaatccta
     5821 acggttagat ggagcggcaa cacagaaaat cctaaaaatt tctcagctgt tgctatagga
     5881 attaatccaa gtaaagcaaa tgatgaaaga agcgacaaaa cccttaccca acttgcacgt
     5941 ttcttggata tgtacggttt taccaacttc aaaatgttaa atattttttc cagttactct
     6001 acacagcaaa caggaattag ggcaaacacc caaaccgatt tttccaaatt taaaggatgt
     6061 ttggaagatg ctgatatgat tattcttgca tggggtacag accgcggtgc ttataaggat
     6121 gaaaaaaaca gaatactgga atttctaaaa gcagaaaaat ttatggaaaa agtattctgt
     6181 atttctgaaa ctggtaattc cagcgataca agacatccgt caagaattag ttatagctat
     6241 caacttgtac aatttgaaga gtcagcatga aaaacatatc tctttctccg cccgccatgc
     6301 ttggcatcct cggcggcgga caattaggca gaatgtttac cgttgccgcc aaaaccatgg
     6361 gctacaaagt aaccgttctc gaccccgatc cggacgcgcc ggcagcagaa tttgccgact
     6421 gtcatttatg cgcgccgttt aacgaccaag ctgctttgga cgaattggca aaatgcgcgg
     6481 cggtgaccac tgaatttgaa aacgtcaatg ccgatgcgat gcgttttctc gccaaacaca
     6541 ccaatgtttc ccccggcagc gactgcgttg ccatcgcgca aaaccgcatt caggaaaagg
     6601 catggatacg caaagcagga ttgcaaaccg cgccgtatca agcaatttgc aaagccgaag
     6661 acatcactga agaaagcata caatttctgc ccggcatcct gaaaaccgct acattgggct
     6721 atgacggcaa aggccaaatc cgcgtcaaaa cggtggatga actcaaagcc gcgtttgccg
     6781 aacaccgcgg cgtggattgc gttttggaaa aaatggtgga cttgcgcggc gaaatttccg
     6841 ttatcgtatg ccgtctgaac aatgacaacg tgcaaacttt cgatcctgcc gaaaacattc
     6901 acgaaaacgg tatcctcgcc tactccatcg tcccagcccg actgagtgcc gacattcagc
     6961 aacaggcgcg acaaatggcg cagcgtttgg ccgatgaatt gaactacgtc ggcgtattgg
     7021 cggtagaaat gtttgttgtc ggcgacacgc atgaattggt cgtcaacgaa atcgcgccgc
     7081 gtccgcacaa ttccggccac cataccgtcg acgcctgcgc ggcagaccaa ttccagcaac
     7141 aggtccgcct gatgtgcaac ctgccacctg ctgacaccaa attgctgagt tcttgctgta
     7201 tggcgaatat tttgggcgac gtttggcagg aagacggcgg cgaaccggat tggtttcccc
     7261 tgcaaagccg gccggacgcg cacctgcacc tttacggcaa aaaaaccgcg cacaaagggc
     7321 ggaaaatggg acactttacc gttttaacca ccgattcgga caccgcattt caagaagcaa
     7381 aaaaactgca tcagtcccta taatccgacc aacggcacag atgccgtctg aagccgccga
     7441 catactgcca tgaacctgct gacactgtta cgccccgcca ccgtgcaaga ctgcaaagac
     7501 atcttcaaag tccacctgca ctccgtccaa tacacctgca tcctcagcta caacgaacac
     7561 gccctaaaag tatgggaagg cttgttaaac acagaaagct acctgccgac catttccgat
     7621 cccgacaaag cactttgggt tgccgaatac aaaggcaaca tacagggatt tttccaaatc
     7681 gactgccaag aagcacaact cgatgcactt tacgtccacc ccctgttcca caacctcgga
     7741 ctgggcactg ccctgcttca tcaggcggaa accatcgccc acaaatccgg tttgagcttt
     7801 ctgaaactct acgcatcgct caactccgtc ccgttttacc ttctcaaccg ctacgaatcg
     7861 ctcggttcgg cagtattgca gcttgacccc agcatcaaaa tcaaatgcga actgatgcgg
     7921 aaacacctct gatccgtcct tataaccaaa actgataatc ttcaaatcaa acattatttc
     7981 cacaagcgca gacagccgta taaagttgcc ccatctacca accgacagga caagcatatg
     8041 aaaacctacg caccggcact ctataccgca gccctgctca ccgcctgcag ccccgcagcc
     8101 gattcaaacc atccgtccgg acaaaatgcc ccggccaata ccgaatccga cggaaaaaac
     8161 attaccctgc tcaacgcctc atacgatgtg gcacgggatt tttacaaaga atacaacccc
     8221 ttatttatca aaacatacca atccgaacac cccggcacat ccgtcagcat ccaacagtcc
     8281 cacggcggct ccagcaaaca ggcattatcc gtagccaacg gccttcaagc cgatgtcgta
     8341 accatgaacc aatcctccga catcgacctg ctcgaaaaaa aaggactggt agaaaaaggc
     8401 tggcaacaag ccctccccga ccacgctgcg ccctacacca gcactatggt tttccttgtc
     8461 cgaaaaaaca accccaaaca gatccgcgat tggaacgacc ttgccaaaga cggcgttaac
     8521 atcgtcatcg ccaatcccaa aacctcgggc aacggacgct acgccttcct cggcgcatac
     8581 ggttacggtc tgaaaaccac caacggcaac gaacaggaag cccaaaaact cgtcgcatcc
     8641 atcctcaaaa acacccccgt ttttgaaaac ggcggacgcg ccgccaccac caccttcaca
     8701 caacgcaaca tcggcgacgt actcattact tttgaaaacg aagccaacta cgtcagcaaa
     8761 aaactgaccc aaggacagtt tgaaatcgtc tatccgagct acaccatttc cgccgaaagc
     8821 cccgtcgccg tcgtcaacag cgtcgtcgcg aaaaaaggca cgcaaaaaac cgcccgcgcc
     8881 tatctcgaat acctttggag cgaaccggcg caagaactcg ccgcctcact ctacctgcgc
     8941 ccccgcaacc ccgaagtatt ggcaagacac aaagccgact tcccagactt agactcattc
     9001 tctcccgaag aaaaattcgg cggatgggac aacatcatga aaacctactt cgccgacggc
     9061 ggcatattcg accgcctgac cgcacaaaaa tgaccgctat gccgtctgaa ccgcctttca
     9121 gacggcattc cacacatcaa aacagaaaaa ccgcgcccaa aatcatcatc acaagacaca
     9181 acggcaaacc ggatacaaaa cccaacaccg ccgccaaaat gccgtctgaa gccgtcggcg
     9241 caaccatcct tatctggggc gcgcttcagg ggcgcataac tttccttaac catccctttt
     9301 gacagccaaa taaacagtgc ttttgcctgt cagaaacaaa accaatattt tgcagttcgt
     9361 atcggaacat ttatagtgga ttaacaaaaa ccagtacagc gttctcgc
//
LOCUS       DAXHSF010000066         9310 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.066, whole genome shotgun sequence.
ACCESSION   DAXHSF010000066 DAXHSF010000000
VERSION     DAXHSF010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9310
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.066"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(102..1382)
                     /locus_tag="ACFM8S_001153"
     CDS             complement(102..1382)
                     /locus_tag="ACFM8S_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HFC6314230.1"
                     /translation="MENSLKEAALKFHELPVPGKISVTPTKSLATDKDLALAYSPGVA
                     APCMEIHADPQNAYKYTAKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFA
                     GVDVFDIEIDEKDPQKLVDIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
                     DDQHGTAIITAAAVLNALRFTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTV
                     CDSKGVIYQTREDKDRMDESKQFYAIEDNGQRVLADAVKGKDIFLGLSGANLLTPEML
                     NTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATTINEEMKRACVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPR
                     VATAAAKAAMESGVATRPIADLEAYAAKLSEWKL"
     gene            complement(1604..2224)
                     /gene="tmk"
                     /locus_tag="ACFM8S_001154"
     CDS             complement(1604..2224)
                     /gene="tmk"
                     /locus_tag="ACFM8S_001154"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFC6314231.1"
                     /translation="MKPQFITLDGIDGAGKSTNLAVIKAWFERRGLPVLFTREPGGTP
                     VGEALREILLNPETKAGLRAETLMMFAARMQHIEDVILPALSDGIHVVSDRFTDATFA
                     YQGGGRGMPSEDIEILEHWVQGGLRPDLTLLLDVPLEVSMARIGQTREKDRFEQEQAD
                     FFMRVRGVYLDRAAACPERYAIIDSNRSLDEVRNSIEKVLDGHFGC"
     gene            complement(2284..3279)
                     /gene="mltG"
                     /locus_tag="ACFM8S_001155"
     CDS             complement(2284..3279)
                     /gene="mltG"
                     /locus_tag="ACFM8S_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFC6314232.1"
                     /translation="MLRKLLKWSAVFLTVSAAVFAALLFVPKDNGRAYRIKIAKNQGI
                     SSVGRKLAEDRIVFSRHVLTAAAYVLGVHNRLHTGTYRLPSEVSAWDILQKMRGGRPD
                     SVTVQIIEGSRFSHMRKVIDATPDIEHDTKGWSNEKLMAEVAPDAFSGNPEGQFFPDS
                     YEIDAGGSDLRIYQTAYKAMQRRLNEAWESRQDGLPYKNPYEMLIMASLIEKETGHEA
                     DRDHVASVFVNRLKIGMRLQTDPSVIYGMGAAYKGKIRKADLRRDTPYNTYTRGGLPP
                     TPIALPGKAALDAAAHPSGEKYLYFVSKMDGTGLSQFSHDLIEHNAAVRKYILKK"
     gene            3363..3935
                     /gene="ampD"
                     /locus_tag="ACFM8S_001156"
     CDS             3363..3935
                     /gene="ampD"
                     /locus_tag="ACFM8S_001156"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFC6314233.1"
                     /translation="MDNHAEAHWQNGWLQSIRHTPSPNFSPRETGETVSLIVLHNISL
                     PPFEYGTDAVEKLFANRLDPDGHPFFSLIHTLRVSSHFLIKRDGETVQFVSCDNMAYH
                     AGVSSFRGREKCNAFSIGIELEGCDFEPFTEAQYRSLEALLDAICRRYPITAVTGHQD
                     IAPGRKTDPGHFFDWRRIRGKGFPVDRNAV"
     gene            4129..5415
                     /locus_tag="ACFM8S_001157"
     CDS             4129..5415
                     /locus_tag="ACFM8S_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HFC6314234.1"
                     /translation="MIYIVLFLAAVLAVVAYNMYQENQYRKKVRDQFGHSDKDALLNS
                     KTSHVRDGQPSGGPVMMPKPQPAVKKTAKLQDPAMRNLQEQDAVYIAKQKQAKASPFK
                     TEIETALEESGIIGNSAHTVSEPQTGHSAPKPADAPAKPVPVPQTPAKPLITLKELSK
                     VELPWFDVRFDFISYIALTEAKELHALPRLSNRCRYQIVGCTMDDHFQIAEPIPGIRY
                     QAFIVGIQAVSRNGLASQEELSAFNRQVDAFAQSMGGQTLHTDLAAFIEVASALDAFC
                     ARVDQTIAIHLVSPTSISGVELRSAVTGVGFVLEDDGAFHYTDTSGSTMFSICSLNNE
                     PFTNALLDNQSYKGFSMLLDIPHSPAGEKTFDDLFMDLAVRLSGQLNLNLVNDKMEEV
                     STQWLKDVRTYVLARQSEMLKVGIEPGGKTALRLFS"
     gene            5563..8088
                     /gene="ligA"
                     /locus_tag="ACFM8S_001158"
     CDS             5563..8088
                     /gene="ligA"
                     /locus_tag="ACFM8S_001158"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFC6314235.1"
                     /translation="MNPNSKHNTNFTNLLKPSDSDIKQFAAQHIRHLTDLLNRYAYEY
                     YTLDAPSVPDAEYDKLFRELEALERNHPELKLPDSPTQRVGGEPLAGFAEVRHEVPML
                     SLTNAFSPQDENGVFDHAEMYAFDQRVRDGLDGGKPEYVIEPKFDGLAISLLYRDGVL
                     VQAATRGDGTTGEDVTQNIKTVSNIPLRLHGENTPELIEVRGEVLMQKADFAALNKRQ
                     AENGQKPFANPRNAAAGSLRQLDSRITAQRKLHFFPYSVARQQGGFVAEEHIQELAYF
                     QALGFSLPNGNFGCFKNIDEVLAFYERMQQKRPKLPYEIDGMVVKVNSLAQQRELGFI
                     SRAPRWAIAHKFPAEEALTIVEAIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQ
                     DEVSRKDVRVGDTVVVRRAGDVIPEVVRVIFERRPMQETAVAVSDGIGHRQDDLFAEA
                     PAANQTQSVPLHKPYRLPTHCPICRSEIEREEGEAVARCSGGMLCQAQRAQGLIHFAS
                     RKAMDIDGLGEKQIEQLVAQDLVRHFADLYRLDIPTLQKMKETTDKTVAESEQMPSEG
                     SSVGASGKHKKPPTKWAENILAGIESGKTPELARFLFALGIRHVGERTAKTLAQAFGT
                     LERVRRAPEPVLACLPDIGTVVAHSIAHFFAQAEQQAMIDELLAAGIAPQTQAVTIPP
                     ARHAEPQRWIARLPGFKISENKAQALWELAGKNIEGLQTDKALPTDWQAWRSEPQNAA
                     LLENLKTFFAQMPSEDEAAQGSDGINEAVAGKTFVLTGTLPTLKRDQAQSLIEAAGGK
                     VSGSVSKKTDYVVAGEAAGSKLEKANALGVSVLSEAELLTLLG"
     gene            8145..>9310
                     /gene="hemW"
                     /locus_tag="ACFM8S_001159"
     CDS             8145..>9310
                     /gene="hemW"
                     /locus_tag="ACFM8S_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFC6314236.1"
                     /translation="MHTISFPNRTRLTALPPLSLYIHIPWCIKKCPYCDFNSHSLKNG
                     LPEAAYIDALLTDLQLELPNIWGRPVETIFFGGGTPSLFQAESIDRLLSGVRSLLRLQ
                     PEAEITLEANPGTFEIEKFQGFKDAGITRLSIGVQSFNDDMLSRLGRVHNGKEALTAI
                     DTALKLFDKVNIDLMYALPNQTVQTALDDVQTAIATGATHISAYHLTMEPNTPFGHTP
                     PKGLPQDEAALDIEDAVHGALEGAGFVHYETSAFAKPAMQCRHNLNYWQFGDYLGIGA
                     GAHGKISYPDRIERTVRRRHPNDYLALMQSQPSEAVERKTVAAEDLPFEFMMNALRLT
                     DGVPAAMLQERTGVPSAKIMAQIETARQKGLLETDPAVFRPTEKGRLFLNDLLQ"
BASE COUNT         2189 a         2818 c         2349 g         1954 t
ORIGIN      
        1 gcttcgtcgc cttgtcctga tttttgttaa tccactatat caatctgaca gcaaaatacc
       61 gcctgaaaac agttcagacg gcattttaaa ccgcaaacgg cttacagctt ccattcgctc
      121 agcttggcag cgtaagcttc caaatctgca atcggacggg ttgccacgcc gctttccatc
      181 gctgctttgg cggcagccgt agcaacgcgc ggcagcaggc gggaatcgaa cggagtcgga
      241 atcaggtatt ccgcgccgaa ttcaaatttc ttaccgtaag cggcaaccac ttcttcggtt
      301 acttcttcca tcgccaaatc cgccaaagca tacacgcagg cgcgtttcat ttcttcgttg
      361 atggtcgtcg cgccgacatc caacgcaccg cggaagatga acgggaagca caatacgttg
      421 ttcacttggt tcgggaagtc ggaacggccg gtaccgataa ccacgtccgg acgggtttct
      481 tttgccagcg gcggcagaat ttccggattc gggttggcca tggcgaacac gatgggtttt
      541 tcgttcatgg tgttcagcat ttcaggcgtc agcaggttcg cgccggagag gcccaagaag
      601 atgtctttgc ctttgacggc atcggcaagc acgcgctggc cgttgtcttc aatggcgtag
      661 aactgtttgg actcgtccat acggtctttg tcttcgcggg tttggtaaat tacgcctttg
      721 gagtcgcaaa cggtcacgtt ttcgcgtttc aagcccaaat ccagcaattg gttcaagcag
      781 gcaatcgcgg ccgcacccgc accagagcaa accaaggtcg cttcttcgat tttacggccg
      841 gtaaaacgca gggcgttcaa tacggcagcg gcggtaatga tggccgtgcc gtgctggtcg
      901 tcgtggaata cggggatttt gcagcgtttg cgtaattcgc gttcgatgta gaaacactcg
      961 ggtgctttga tgtcttcgag gttgatgccg ccgaaggtcg gctctaaagc ggcgatgatg
     1021 tccacgagtt tttgcgggtc tttttcatcg atttcgatgt cgaacacgtc cacaccggcg
     1081 aattttttga acaatacgcc tttgccttcc atcacgggtt tgcccgccag cgcgccgatg
     1141 tcgcccaagc ccaaaacggc cgtaccgttg gaaatgacag cgaccaagtt gcctttggcg
     1201 gtgtatttgt aggcattttg cggatcggca tggatttcca tacaaggagc ggctacgccc
     1261 ggagagtacg ccaacgccaa atctttgtcg gtcgccagag atttggtcgg ggtaacggaa
     1321 attttgcccg gaacgggtaa ttcgtggaac ttgagggcgg cttctttcaa tgagttttcc
     1381 atgtgtcaat cctgttgcga gaaaaagatt tgaatcggac tgtccgaaac gctttcgggt
     1441 catcatgtta cgggcggttc gttaatattg ccgacgcatc ctgccttgta accgagcgta
     1501 attctatcat gccgaaagtt tggcgcatac cgccattttc acctgcggca ctgccgtttg
     1561 attttcggcg cgggcggatg cgcttgtttc aatatttgcc gcatcagcag ccgaaatgtc
     1621 cgtccaacac tttttctatg ctgtttctga cttcatccaa gctgcggtta ctgtcgataa
     1681 tggcgtaccg ttcgggacag gcggcggctc ggtcgagata aacgccgcgc acacgcatga
     1741 agaaatctgc ctgttcctgc tcgaaacggt ctttctcgcg cgtctgcccg atacgcgcca
     1801 tcgacacttc gagtggcaca tccaacagca gggtcaaatc ggggcgcaaa ccgccctgca
     1861 cccaatgttc caaaatttca atgtcttcag acggcatccc ccgcccgcca ccctgatagg
     1921 cgaaggttgc gtcggtaaaa cggtcggaca cgacatggat gccgtctgaa agtgcaggca
     1981 atatcacgtc ttcaatatgc tgcattctgg cggcgaacat catcagggtt tccgcacgca
     2041 aaccggcttt ggtttcaggg ttgagcagga tttcgcgcaa ggcctcaccg accggcgttc
     2101 cgcccggttc gcgcgtgaac agcacgggca gccccctccg ttcaaaccat gccttgatga
     2161 cggcaaggtt ggtggatttg ccggcaccgt ctatgccgtc caaagtgatg aattgcggtt
     2221 tcatgcttgc agttccggta agggtatgcc gtctgaaaac acaaactttt cagacggcat
     2281 ggtttatttt ttcaaaatat atttgcgaac ggcggcgttg tgttcgatca aatcatggct
     2341 gaactggctc aagcccgtac cgtccatttt agaaacaaaa taaaggtatt tttcgccgga
     2401 cggatgggcg gcggcatcaa gtgccgcctt gccgggcagc gcgatcgggg ttggcggcag
     2461 accgccgcgc gtgtaggtgt tgtacggcgt gtcgcggcgc aggtcggctt tacggatttt
     2521 gcccttgtat gccgcaccca tgccgtaaat cacggacggg tcggtttgca gacgcatacc
     2581 gattttcagg cggttgacga agacggaagc gacatggtcg cggtcggctt catgccctgt
     2641 ttccttttcg atcaggctcg ccataatcag catttcataa ggatttttat aaggcagccc
     2701 gtcctgcctg ctttcccatg cctcattcag gcggcgttgc atcgccttgt aggcggtttg
     2761 gtaaatccgt aaatcgctgc cgcccgcatc gatttcgtag ctgtcgggga aaaactgccc
     2821 ttcaggattg ccgctgaagg catcaggggc aacttccgcc atcagttttt cattgctcca
     2881 gcctttggtg tcgtgttcga tgtcgggcgt tgcgtcgatg actttcctca tatgcgaaaa
     2941 acgcgaacct tcgataatct gcacggtaac ggaatccggc ctgccgccgc gcattttctg
     3001 caagatatcc caagcagaca cttccgaagg caatctgtac gtccccgtat gcagcctgtt
     3061 gtgcacaccc aaaacgtagg ccgccgccgt caaaacatgc ctgctgaaca cgatgcggtc
     3121 ttcggcaagt ttcctgccga ccgacgaaat accctggttt ttggcaattt taatcctgta
     3181 tgccctgccg ttgtctttag ggacgaaaag cagcgcggcg aaaacggctg ccgatacggt
     3241 caaaaaaacg gcagaccatt tcaacaattt tctcaacatg gtaggattcc cagcattcgg
     3301 aatacgtctt aaaattcggg cgacagtata acccaaattg cccgccggac aggaagaaca
     3361 tcatggacaa tcacgccgaa gcacactggc aaaacggctg gcttcaaagc atacgccata
     3421 ccccgtcgcc caatttcagc ccgagggaaa cgggggaaac ggtttccctg atcgtgttgc
     3481 acaacatttc gctgccgccg ttcgaatatg gtacggatgc ggtggaaaag ctgtttgcca
     3541 accggctcga ccccgacgga catccgttct tcagcctgat acacactttg cgcgtatcca
     3601 gccatttttt aatcaaacgc gacggcgaaa cggtacagtt cgtttcctgc gacaatatgg
     3661 cataccatgc cggcgtatcc tcgtttcgcg gacgggaaaa atgcaacgca ttttccatcg
     3721 gcatcgaact ggaaggctgc gatttcgaac cctttaccga agcgcaatac cgttcgctcg
     3781 aagccttgtt ggacgcaatc tgccgccgct accccatcac ggcggtaacg gggcaccaag
     3841 acatcgcgcc cggccgcaaa accgaccccg gtcatttttt cgactggcgg cggatacggg
     3901 ggaaagggtt tcccgtggac agaaatgccg tctgaaaacc acagatttta gacggcattt
     3961 cggaaaattt ccgcttattt cgacacactg ctgtcagacc ctgcaatttt cgttataatt
     4021 caaacgttat aaccggccgt tccatcagac tgccggaaac gcaagcccga tttaaccgca
     4081 gtacccgcac ttcgcgcatc ctgcatcccg aaatttaaag tgattgccat gatttacatc
     4141 gtactgttcc tcgccgccgt cctcgccgtt gtcgcctaca atatgtatca ggaaaaccaa
     4201 taccgcaaaa aagtgcgcga ccagttcgga cactccgaca aagatgccct gctcaacagc
     4261 aaaaccagcc atgtccgcga cggccaaccg tccggcgggc cagtcatgat gccgaaaccc
     4321 caaccggcgg tcaaaaaaac ggcaaaactc caagaccctg ctatgcgcaa cctgcaagaa
     4381 caggatgccg tctacatcgc caagcagaaa caggcaaaag cctccccgtt caaaaccgaa
     4441 atcgaaaccg ccttggaaga aagcggcatt atcggcaact ccgcccacac cgtttccgaa
     4501 ccccaaaccg gacattccgc accgaaacct gccgacgcgc cggcaaaacc cgttcccgtt
     4561 ccgcaaacgc cggcaaaacc gctgattacg ctcaaagagc tgtcgaaggt cgagctgccc
     4621 tggtttgacg tgcgcttcga cttcatctct tatatcgcgc tgaccgaagc caaagaactt
     4681 cacgcactgc cgcgcctttc caaccgctgc cgctaccaga ttgtcggctg caccatggac
     4741 gaccatttcc agattgccga acccatcccg ggcatccgct atcaggcatt tatcgtgggt
     4801 attcaggcag tcagccgcaa cggacttgcc tcgcaggaag aactctccgc attcaaccgc
     4861 caggtggacg cattcgcaca aagcatgggc ggtcagacgc tgcacaccga ccttgccgcc
     4921 tttatcgaag tggcttccgc actggacgca ttctgcgcgc gcgtcgacca gaccatcgcc
     4981 atccatttgg tttccccgac cagcatcagc ggcgtagaac tgcgttccgc cgtaacgggc
     5041 gtgggtttcg ttttggaaga cgacggcgcg ttccactata ccgacacgtc gggctcgacc
     5101 atgttctcca tctgctcgct caacaacgag ccgtttacca atgccctttt ggacaaccag
     5161 tcctacaaag gcttcagtat gctgctcgac atcccgcact ctccggcagg cgaaaaaacc
     5221 ttcgacgatt tgtttatgga tttggcggta cgcctgtccg gccagttgaa cctgaatctg
     5281 gtcaacgaca aaatggaaga agtttcgacc caatggctca aagacgtgcg cacttatgta
     5341 ttggctcgtc agtccgagat gctcaaagtc ggtatcgaac cgggcggcaa aaccgcattg
     5401 cgcctgttct cctaaaatcg aatgcaatca aagcggatat gtcgttacgg catatccgct
     5461 ttttttcggg taaaattcag ctgttttcaa cctatcattt ttcagacgac ctttgcccgt
     5521 ttcatcccgt atcagacaaa ggtcgtctga aaaccttctc tcatgaatcc aaactcaaaa
     5581 cataacacta actttaccaa tcttcttaaa ccgtctgata gtgatataaa acaatttgcc
     5641 gcacaacaca tccgccacct caccgacctg ctcaaccgct atgcctacga atactacacc
     5701 ctcgacgcgc ccagcgtacc cgatgccgaa tacgacaaat tattccgcga gctcgaagcg
     5761 ttggaacgaa accatcccga gctcaaactg cccgacagcc cgacccagcg cgtcggcggc
     5821 gagcctctgg cgggatttgc cgaagtacgc cacgaagtgc cgatgctgtc gctgaccaac
     5881 gccttttccc cgcaagatga aaacggcgtg ttcgaccatg cagaaatgta cgctttcgac
     5941 caacgcgtgc gcgacggttt ggacggcggc aaacccgaat acgttatcga acctaaattc
     6001 gacggcctcg ccatcagcct gctctaccgc gacggcgtat tggtacaggc ggcaacgcgc
     6061 ggcgacggca caacgggcga agacgttacc caaaacatca aaaccgtatc caacatcccc
     6121 ttgcggctgc acggcgaaaa tacgcccgaa ctcatcgaag tacgcggcga agtactgatg
     6181 caaaaagccg attttgccgc cctcaataaa agacaagccg aaaacgggca aaaacccttt
     6241 gccaatccgc gcaacgccgc tgccggcagc ctgcgccaac tcgattcgcg catcactgcg
     6301 caacgcaaac tgcacttttt cccctactcc gtcgcccgcc agcaaggcgg tttcgtcgcg
     6361 gaagaacaca tccaagaact cgcctatttc caagcactcg gcttcagtct gcccaatggc
     6421 aatttcggct gtttcaaaaa tatcgacgaa gtattagcgt tttacgaacg tatgcagcaa
     6481 aaacgcccca aactgcccta cgaaatcgac ggcatggtgg tcaaagtcaa cagcttggcg
     6541 caacagcgcg aactcggctt catctcccgc gcgccgcgct gggcgattgc ccacaaattc
     6601 cccgccgaag aagccctgac cattgtcgaa gcgattgacg tgcaaatcgg caggacgggc
     6661 gcggtaacgc cggttgcccg attacaaccc gtattcgtcg gtggcgtaac tgttaccaac
     6721 gccaccctgc acaatcagga cgaagtatcg cgtaaagacg tgcgcgtcgg cgataccgtc
     6781 gtcgtgcgcc gcgccggaga cgtgattccc gaagttgtgc gcgtgatttt tgaacgccgc
     6841 ccgatgcagg aaaccgccgt tgctgtttca gacggcatcg ggcatcggca ggacgatttg
     6901 tttgccgaag caccggctgc aaaccaaacc caatccgttc cgctccacaa gccctaccgc
     6961 ctgccgaccc actgccccat ctgccgcagc gaaatcgaac gcgaagaagg cgaagccgtc
     7021 gcccgatgca gcggcggtat gctttgtcag gcacaacgcg cgcaaggctt aatccacttc
     7081 gcctcgcgca aagccatgga catcgacggc ttgggtgaaa aacaaatcga acagcttgtc
     7141 gcccaagacc tcgtccgcca cttcgccgac ctctaccgcc tcgatattcc gaccctgcaa
     7201 aaaatgaagg aaacgacgga caaaaccgtt gccgaatcgg agcaaatgcc gtccgaaggc
     7261 agttcggtcg gcgcgtcggg caaacataaa aaaccaccga ccaagtgggc ggaaaacatc
     7321 ctcgcaggca tagaatccgg caaaacgccc gaactcgcgc gcttcctgtt cgcgctcggc
     7381 atccgccacg tcggcgaacg caccgccaaa acgctggcgc aggcattcgg cacattggaa
     7441 cgcgtccgcc gcgcccccga acccgtcctc gcctgcctgc ccgacatcgg tacggtagtg
     7501 gcgcattcca ttgcccactt cttcgcccaa gccgaacagc aggcgatgat agacgaactg
     7561 ctcgccgcag gcattgcccc gcaaacccaa gccgttacca tcccgcccgc ccgccacgcc
     7621 gaaccgcaac gctggatcgc ccgcctgccc ggtttcaaaa tcagcgaaaa caaagcccaa
     7681 gccttatggg aactcgccgg caaaaacata gaagggctgc aaaccgacaa agccctcccc
     7741 accgactggc aggcgtggcg cagcgaacca caaaacgccg ccctgctcga aaacctgaaa
     7801 accttcttcg cgcaaatgcc gtctgaagac gaggcggcgc aaggttcaga cggcatcaac
     7861 gaagccgtag caggcaaaac ctttgtatta accggcaccc tgcccacgct caaacgcgac
     7921 caagcccaat ccctaatcga agccgcaggc ggcaaagttt ccggcagcgt gtccaaaaaa
     7981 accgactacg tcgtcgccgg agaagccgcg ggcagcaagc tggaaaaagc caatgccttg
     8041 ggcgtttccg tcctcagcga agccgaactg cttaccctgc tcggctgaac ctatgccgtc
     8101 tgaaccgcct tcagacggca ttgtccactt ttccgaacca caccatgcac accatttcct
     8161 ttccaaaccg cacccgactc accgccctgc cgcccctgtc gctttacatc cacatcccgt
     8221 ggtgcatcaa aaaatgcccg tattgcgact tcaattccca cagcctgaaa aacggattgc
     8281 ccgaagccgc ctatatcgac gcgctgctga ccgatttgca gcttgaattg cccaatattt
     8341 ggggcaggcc ggtggaaacg atctttttcg gcggcggtac gcccagcctg tttcaggcgg
     8401 aatcgattga ccgtttgtta agcggcgtgc gttcgctgtt gcgcttgcag ccggaagcgg
     8461 aaattacctt ggaagccaat ccggggacat ttgaaatcga gaagtttcaa ggatttaaag
     8521 acgcgggtat tacgcggctt tctatcggcg tacagagttt caacgacgat atgctctcaa
     8581 ggctggggcg cgtccacaac ggtaaggaag ccttaaccgc tatcgatacc gccttgaaat
     8641 tatttgataa agtcaatatc gacttgatgt atgccctgcc gaaccaaacc gttcagacgg
     8701 cattggacga cgtacaaacc gccatcgcta cgggcgcgac ccacatcagc gcgtatcatc
     8761 tgacgatgga accgaacacg ccgttcggtc atacgccgcc gaaaggtttg ccgcaagacg
     8821 aagccgccct cgacatcgaa gacgcggtac acggcgcgct ggaaggcgcg ggttttgtcc
     8881 actacgaaac atcggctttt gcgaaaccag ccatgcagtg ccgccacaat ttgaactact
     8941 ggcagttcgg cgattattta ggcataggcg cgggcgctca cggcaaaatt tcctatcccg
     9001 accgcatcga gcgcaccgtc cgccgccgcc accccaacga ctacctcgcc ttaatgcaaa
     9061 gccaaccgag tgaagccgtc gaacgcaaaa ccgttgccgc cgaagatttg ccgttcgagt
     9121 ttatgatgaa cgccctgcgc ctgaccgacg gcgtacccgc cgcgatgttg caggagcgca
     9181 cgggcgtacc gagtgccaaa atcatggcgc aaatcgaaac ggcaaggcaa aaaggcctgc
     9241 tggaaaccga ccccgccgta ttccgcccga ccgaaaaagg acgcttgttt ttaaacgatt
     9301 tgctgcaatg
//
LOCUS       DAXHSF010000067         9257 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.067, whole genome shotgun sequence.
ACCESSION   DAXHSF010000067 DAXHSF010000000
VERSION     DAXHSF010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9257
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.067"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(<1..191)
                     /locus_tag="ACFM8S_001160"
     CDS             complement(<1..191)
                     /locus_tag="ACFM8S_001160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314237.1"
                     /translation="MPSENLSDGIFYQSSHIFYQGCKIYPKQQKPPCRHSRKSGNLEP
                     NTAKIYLKRQQSFHRHSRAG"
     gene            complement(208..1146)
                     /locus_tag="ACFM8S_001161"
     CDS             complement(208..1146)
                     /locus_tag="ACFM8S_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC6314238.1"
                     /translation="MLELNGLCKRFGGKTVADDICLTVGRGKILAVLGRSGCGKSTLL
                     NMIAGIIRPDGGEIRLNGENITRMPPEKRRISLMFQDYALFPHMSALENAAFGLKMQK
                     MPKAEAERLAMAALAEVGLENEVHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFS
                     SLDTHLRDRLRRMTAERIRNGGIPAVLVTHSPEEACTTADEIAVMHEGRILQYGTPET
                     LVQTPAGVQVARLMGLPNTDDDRHIPQNAVRFDQDGMECRVLSRTCLPESFSLSVLHP
                     EHGILWLNLDMRHAGAVSGKDTVRIHIEEREIVRFR"
     gene            complement(1155..2030)
                     /locus_tag="ACFM8S_001162"
     CDS             complement(1155..2030)
                     /locus_tag="ACFM8S_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFC6314239.1"
                     /translation="MPTVRFTESVSKHDLDALFEWAKASYGAESCWKTLYLNRLPLGN
                     LSPEWAERVKKDWEAGCSESSDGIFLNADGWPDMGRRLQHLARTWNKAGLLHGWRNEC
                     FDLTDGGGNILFSLERAAFRPFGLLSRAVHLNGLTESDGRWHFWIGRRSPHKAVDPDK
                     LDNIAAGGVSSGELPSEAVCRESSEEAGLDQTLFPLIRPVSQLHSLRPVSRGVHNEIL
                     YVFDAVLPETFLPENQDGEVACFEKMDIGSLVAAMLSENMMHDAQLVTLDAFCRYGLI
                     DVAHPLSEWLDGIRL"
     gene            complement(2213..2289)
                     /locus_tag="ACFM8S_001163"
     tRNA            complement(2213..2289)
                     /locus_tag="ACFM8S_001163"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2253..2255),aa:Met,seq:cat)
     regulatory      2439..2538
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2629..4530
                     /gene="thiC"
                     /locus_tag="ACFM8S_001164"
     CDS             2629..4530
                     /gene="thiC"
                     /locus_tag="ACFM8S_001164"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFC6314240.1"
                     /translation="MTTPKKTAKTSGNEARELADLSEDIGIRFKYPNSERVYLQGSRD
                     DIRVPLREIRQDDTYTAQGTEANPPIPVYDTSGVYGDPAAHIDLKQGLPHIRTAWLNE
                     RGDTEILPKLSSEYGIERAHDPKTAHLRFNQITRPRRAKSGSNVTQLHYARQGIITPE
                     MEFVAIRERLKLDELSQKPEYAKLLKQHAGQSFGANIPTHPDQITPEFVRQEIAAGRA
                     IIPANINHPELEPMIIGRNFRVKINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDL
                     STGAHIHETREWIIRNAPVPIGTVPIYQALEKTGGIAEDLTWDLFRDTLIEQAEQGVD
                     YFTIHAGVLLRYVPMTANRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAY
                     DVSFSLGDGLRPGCIADANDESQFAELHTLGELTDKAWKHDVQVMIEGPGHVPLQRVK
                     ENMTEELQHCFEAPFYTLGPLVTDIAPGYDHITSGIGAANIGWYGTAMLCYVTPKEHL
                     GLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFRLSLDPE
                     RAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEF
                     VKKGAKIYS"
     gene            complement(4777..6039)
                     /locus_tag="ACFM8S_001165"
     CDS             complement(4777..6039)
                     /locus_tag="ACFM8S_001165"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFC6314241.1"
                     /translation="MSNTQKQLALAKAAKKSVNTADTEEKNRALLAMADSLEAAAADI
                     LAANRQDLEAAAGKIPESMTDRLLLDGKRICAMADGIRAVAALPDPVGEILETSTLPN
                     GLEIVKKRVAMGVIGIIYESRPNVTSDAAALALKSGSAVVLRSGKDAFQSARAIVAAL
                     KAGLAQTRIDPDALQLIEDTGREGSYEMMRAKDYLDLLIPRGGAGLIRAVVENAVVPV
                     IETGTGIVHIYIDKDADWDKALRIVYNAKTSRPSVCNSMEVLLVHEDIAADFLPKLER
                     LLVRDRIEAGLPPVRFRLDPQAARHIGGEAAGADDFDTEFLDYILAVKTVASVEEAVG
                     HIEAHGTHHSDGIVTENRHAADYFTTHIDSAAVYVNASTRFTDGGEFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYIVQGTGQVRE"
     gene            complement(6052..7161)
                     /gene="proB"
                     /locus_tag="ACFM8S_001166"
     CDS             complement(6052..7161)
                     /gene="proB"
                     /locus_tag="ACFM8S_001166"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFC6314242.1"
                     /translation="MKYKRIVFKVGTSSITHSDGSLSRGKIQTITRQLAALHHAGHEL
                     VLVSSGAVAAGFGALGFKKRPVKIADKQASAAVGQGLLMEEYTANLSSDGIVSAQILL
                     SRADFADKRRYQNAGGALSVLLQRRAVPIINENDTVSVEELKIGDNDTLSAQVAAMIQ
                     ADLLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIEMAGGSGSANGTGGMLTKIK
                     AATIAAESGVPVYICSSLKPDALAEAAEHQADGSFFVPRAKGLRTQKQWLAFYSESRG
                     SVYVDEGAEHALSEQGKSLLMSGIAGIEGHFSRMDTVTVYSKATKQPLGKGRVLFGSA
                     AAEDLLKSRKAKGVFIHRDDWISITPEIRLLLTEF"
     gene            7407..9200
                     /locus_tag="ACFM8S_001167"
     CDS             7407..9200
                     /locus_tag="ACFM8S_001167"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFC6314243.1"
                     /translation="MQLDIDRLVAYFGGVNALAEALKQHDPENAATTAAIYKWRTRGS
                     LPLAQLQKLTALAESQGRPLDLNAFLQKNESLERTEMTQANRVIIFDTTLRDGEQSPG
                     AAMTKEEKIRVARQLEKLGVDIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIE
                     RDIRQAGEAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAREYTDDV
                     EFSCEDALRSEIDFLAEICGAVIEAGATTINIPDTVGYSIPYKTEEFFRELIAKTPNG
                     GKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNASVEEIVMALKVRH
                     DLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRET
                     YEIMSAESVGWSANRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFARFKELADKK
                     REIFDEDLHALVSDEMGNMNTESYKFISQKISTETGEEPRADIVFSIKGEEKRASATG
                     SGPVDAIFKAIESVAQSGATLQIYSVNAVTQGTESQGETSVRLARGNRVVNGQGADTD
                     VLVATAKAYLSALSKLEFSAAKPKAQGSGTI"
BASE COUNT         2141 a         2597 c         2489 g         2030 t
ORIGIN      
        1 cctgcgcggg aatgacgatg aaaagattgt tgtcgcttca gataaatttt tgccgtgttg
       61 ggttctagat tcccgctttt gcgggaatga cggcagggtg gtttctgttg tttcggataa
      121 attttgcagc cctgataaaa aatatggctg ctttggtaaa aaatgccgtc tgaaaggttt
      181 tcagacggca tttgttttta agaagcatca gcggaagcgg acgatttccc gttcttcgat
      241 atggatgcgt accgtatcct tgcccgatac cgccccggcg tgccgcatat cgaggttcag
      301 ccacaggatg ccgtgttccg gatggaggac ggacaggctg aacgattcgg gcaaacaggt
      361 acgggataat acgcggcact ccatgccgtc ttggtcgaaa cgcaccgcat tttgcgggat
      421 atggcggtca tcgtcggtgt tgggcagccc catcagacgg gcgacctgca cgccggcagg
      481 cgtttgaacc aaggtttcgg gtgtgccgta ttgtagaatc ctcccttcat gcatcacggc
      541 gatttcgtct gccgtcgtac aggcttcttc gggcgaatgc gttaccaaaa cggcagggat
      601 gccgccgttt cggatacgtt cggcagtcat gcggcgcagc cggtcgcgca aatgcgtgtc
      661 caaactggaa aacgattcgt ccaacagcag cagggaaggg cggacaacca aagcgcgcgc
      721 cagtgccaac cgttgcttct cgcccccgga aagtttttca ggcttgcggt gcacctcgtt
      781 ttccagtccg acttcggcaa gtgccgccat ggcaaggcgt tcggcttcgg ctttcggcat
      841 tttttgcatt ttcaaaccga atgccgcatt ttccagcgca ctcatatggg gaaacagcgc
      901 gtaatcttga aacatcagcg aaatacggcg tttttcgggc ggcatacggg taatgttttc
      961 cccgttcagc cgaatttcgc cgccgtccgg acggataatc cccgcaatca tattcagcag
     1021 tgttgatttt ccgcagcccg accgccccaa aacggcgagt attttgccgc gcccgacagt
     1081 caggcagata tcgtcggcaa ccgttttgcc gccgaagcgt ttgcagagtc cgttcagttc
     1141 aagcatggcg catcctataa acgtatgccg tccagccact cggacagcgg atgggcgaca
     1201 tcaatcagac cgtaacggca aaacgcgtcc agcgtaacca gttgcgcgtc gtgcatcatg
     1261 ttttccgaca acatggcggc aaccaggctg ccgatgtcca ttttctcaaa acacgcaacc
     1321 tcgccgtcct gattttcagg caggaaggtt tcgggcagga cggcatcgaa cacatacagg
     1381 atttcattgt gcacaccccg gctgacgggg cgcaggctgt gcagctgcga taccgggcgg
     1441 atgagcggaa acagcgtttg atccaaaccc gcttcttcgc tgctttcgcg acacacggct
     1501 tcagacggca attcaccgct ggaaacaccg ccggcggcaa tattgtcgag tttgtcggga
     1561 tcgactgctt tgtgcggact gcgcctgcct atccaaaaat gccatctgcc atccgattca
     1621 gtcagaccgt tgagatggac ggcgcggctg agcagtccga acggacggaa agcggcgcgt
     1681 tcgagtgaga acaagatatt gccgccgccg tcggtcaggt cgaaacactc gttgcgccat
     1741 ccgtgaagca gccccgcctt gttccatgtg cgggcgaggt gctgcaagcg tctgcccata
     1801 tctggccagc cgtccgcatt caggaaaatg ccgtctgaag actccgagca gcctgcctcc
     1861 cagtcttttt tgacgcgctc cgcccattcc ggcgacagat tgcccaaagg cagacggttc
     1921 agatacagcg ttttccagca actttctgca ccgtaacttg cttttgccca ctcgaacagg
     1981 gcatcaaggt cgtgtttgct gacggattcg gtaaaacgga cggtcggcat agtcggcttt
     2041 cttgaagaca atgcggaaga ttgtagccaa accgcctgaa tttgcaaccg caaacagacg
     2101 gcgggctgcc gttccgatat tgcggcagga aggaaaatcc ggcaaaaaac ggaggtggca
     2161 ggaaacaggc aaaacaaaac ccctcggcat tgaaccgagg ggttttaata tttggttgcg
     2221 ggagcaggat tcgaacctac gaccttcggg ttatgagccc gacgagctac catgctgctc
     2281 catcccgcgt tagaagatga gactatacgg cagatttttt atgttgtcaa acattacttc
     2341 cgcgaaaaat cataaaattt tgccgtccgg cggtttatgc ctgatttgac atattatttt
     2401 ctttacaaaa gttcatgttc gtgatttaat tttggttaac attgaaacag gggtgctgcc
     2461 tgatgttcag gcggctgaga aatacccttt acacccgatc gggataatac ctgcgtgggg
     2521 agttttcacg gattctgtct ttcagacggc attggttttc aaatgccgtc tgaaaacgca
     2581 aaacgctcct gtttctttat tctaaacgag aaaacaggag cattttttat gactacgcca
     2641 aaaaaaaccg ccaaaacttc cggcaacgaa gcgcgcgaac ttgccgactt gagcgaagac
     2701 atcggcatcc gcttcaaata tccgaactcg gagcgcgtgt atctgcaagg cagccgcgac
     2761 gacatccgcg tgcctttgcg tgaaatccgt caggacgaca cttacacggc gcaaggtacg
     2821 gaagccaatc cgccgattcc cgtctatgac accagcggcg tgtatggcga tccggcggcg
     2881 cacatcgacc tgaaacaagg cctgccgcac atccgcactg catggctgaa cgaacgcggc
     2941 gataccgaaa tcctgcccaa gctctccagc gaatacggca tcgaacgcgc acacgatccg
     3001 aaaaccgccc atctgcgctt caaccaaatc acccgcccgc gccgcgcgaa aagcggcagc
     3061 aacgtaaccc agctccatta cgctcgccaa ggcattatca cgcccgaaat ggaatttgtc
     3121 gccatacgcg aacgtttaaa attagacgaa ttgtcccaaa aaccggaata cgccaagctc
     3181 ttgaaacaac acgcagggca aagtttcggc gcgaacatcc cgactcatcc cgaccaaatc
     3241 acgcccgaat tcgtgcgcca agaaatcgcc gccggacgcg caattatccc tgccaacatc
     3301 aaccaccccg aactcgaacc gatgattatc ggccgcaact tccgcgtcaa aatcaacggc
     3361 aacttgggca attccgccgt cacttccagc ctgaccgaag aagtcgaaaa aatggtgtgg
     3421 tcgctgcgtt ggggcgcgga cacgattatg gatttgtcca ccggcgcgca catccatgaa
     3481 acgcgcgaat ggattatccg caacgcgccc gtccccatcg gcaccgtgcc gatttaccaa
     3541 gcgttggaaa aaaccggcgg catcgccgaa gatttgactt gggatttgtt ccgcgacacc
     3601 ctcatcgaac aggcggagca aggcgtggac tattttacca tacacgcggg cgtgttgctg
     3661 cgttatgtgc cgatgaccgc caaccgcctc accggcatcg tctcgcgcgg cggttcgatt
     3721 atggcgaaat ggtgtttggc acatcatcgg gaaaatttcc tctacacgca tttcgacgaa
     3781 atctgcgaaa ttatgaaagc gtatgacgta tcgttcagcc tcggcgacgg cctgcgcccc
     3841 ggctgcattg ccgatgccaa cgacgaatcc caattcgccg aactgcacac cttgggcgaa
     3901 ttgaccgata aagcgtggaa acatgacgta caagtcatga tcgaaggccc cggccatgtg
     3961 ccgctgcaac gcgtcaaaga aaatatgacc gaagagctgc aacactgttt tgaagcgccg
     4021 ttttacacac tcggcccgct cgttaccgac atcgcccccg gctacgacca catcacctcg
     4081 ggcataggcg cggccaatat cggctggtac ggcacggcga tgctttgtta cgttaccccc
     4141 aaagaacatt tggggctgcc cgacaaagaa gacgtgcgca ccggcatcat cacctacaaa
     4201 ctcgccgccc acgccgccga tctcgccaaa ggctggccgg gcgcacagtt gcgcgacaac
     4261 gccctgagca aggcgcgttt cgagttccgt tggcgcgacc aatttcgctt aagcctcgac
     4321 cctgaacgtg ccgagagctt ccacgacgaa accctgcctg ccgaaggcgc gaaaatcgcc
     4381 cacttctgct caatgtgcgg ccccaaattc tgctcgatga aaatcacgca ggaagtgcgc
     4441 gactacgccg acaagcaaaa agcccaacgg cagggcatgg aggaaaaagc ggtcgagttc
     4501 gtcaaaaaag gcgcgaagat ttacagttga aacgtcaagc aaaaaatgcc gtctgaaacc
     4561 cggaaaaaag gcttcagacg gtattctttc gcttgtgaaa atatagtgga ttaacaaaaa
     4621 tcaggacaag gcgacgaagc cgcagacagt acagatagta gggaaccgat tcacttggtg
     4681 cttcagcacc ttagagaatc gttctctttg agctaaggcg aggcaatgcc gtatcggttt
     4741 aaagttaatc cactatatca gacgtttgaa ttcggattat tccctaacct gtcccgtgcc
     4801 ttgtacgatg tatttgtaac tcgtcagctc tttcaaaccc atcggacccc gggcgtggag
     4861 tttttgcgtg gagatgccca tttcgcaacc caagccgaat tcgccgccgt cggtaaagcg
     4921 cgtggacgcg ttgacataca cggcggcaga atcgatatgg gtcgtgaaat agtccgcagc
     4981 gtggcggttt tcggtaacga tgccgtctga atggtgtgtg ccgtgggctt cgatgtgccc
     5041 gaccgcctct tcgaccgatg cgacggtttt cacagcgagg atgtagtcta aaaactcggt
     5101 atcgaaatcg tctgcacccg ccgcttcgcc gccgatatgc cgcgccgcct gcggatccaa
     5161 acggaagcgg acgggcggca gtccggcttc tatgcggtcg cgaaccaaca gccgttcgag
     5221 cttgggcagg aagtcggcag caatgtcttc atgcaccagc agcacttcca tcgagttgca
     5281 cacggacgga cggctggttt tggcgttgta cacgatacgg agcgccttgt cccaatccgc
     5341 gtccttgtcg atataaatgt ggacgatgcc cgtccccgtt tcaatgaccg gcacgacggc
     5401 attttcaacc accgcccgta tcagccccgc cccgccgcgc ggaatcagca ggtctagata
     5461 atctttcgcc ctcatcattt cgtaactgcc ctcacgccct gtgtcttcaa tcaactggag
     5521 cgcgtcgggg tcgatgcggg tttgcgccaa accggctttg agtgcggcaa cgatggcgcg
     5581 tgcggattgg aatgcatctt tgccgctgcg gagtacgacc gcgctgccgc ttttcagtgc
     5641 caaagccgcc gcatcggacg taacgttcgg gcggctttcg taaataatgc cgataacgcc
     5701 catcgccaca cgctttttga cgatttccaa gccgttgggc agagtcgagg tttccagtat
     5761 ttcgcccacg gggtcgggca gtgcggcaac cgccctaatg ccgtccgcca tcgcgcaaat
     5821 gcgtttgccg tccaacaaaa ggcggtcggt catgctttcg ggaattttgc ctgccgcggc
     5881 ttccaagtct tgacggtttg ccgccaaaat atctgccgcc gccgcttcca gactgtccgc
     5941 catagcaagc agcgcgcggt ttttttcttc cgtatccgcc gtgttgacgg atttttttgc
     6001 cgctttggca agggcaagct gtttttgtgt gtttgacatg ggtttccttt tctaaaattc
     6061 ggtcagaagc aggcgtattt cgggcgtgat ggaaatccag tcgtcccgat ggatgaacac
     6121 gcctttcgcc ttacgcgatt tgagcaggtc ttcggcggcg gcagaaccga acaggacgcg
     6181 ccctttgccc aggggctgtt tggttgcctt gctgtacacg gttacggtgt ccatacggga
     6241 aaaatgccct tcgattccgg caatgcccga catcagcagg cttttcccct gttcggacaa
     6301 agcgtgttcc gcaccttcgt ccacataaac gctgccccgg ctttcggaat agaacgccag
     6361 ccattgcttc tgcgtccgca aacctttggc acgggggacg aaaaacgagc cgtccgcctg
     6421 atgttcggca gcttcggcaa gtgcatcggg tttgagcgag gaacagatat acaccggtac
     6481 gccggattcg gcggcgatgg ttgccgcttt gattttggtc agcataccgc ccgtgccgtt
     6541 tgccgaaccc gatccgcccg ccatttcgat gatttcatgg ttgatgtgtt cgattttgtc
     6601 cagccgtacg gcatcgggat tgctgttcgg gttgcccgtg taaagaccgt ctatgtcggt
     6661 cagcagcacc aagaggtctg cctgtatcat cgccgccact tgcgcactca atgtgtcgtt
     6721 gtcgccgatt ttcaattcct caaccgaaac cgtatcgttt tcattgatga tggggacggc
     6781 gcggcgttgc agcagcacgg aaagtgcgcc gccggcattt tggtagcggc gtttgtcggc
     6841 aaagtcggcg cggctgagca ggatttgtgc ggacacgatg ccgtctgaag acaggtttgc
     6901 cgtatattct tccatcagca gcccctgccc gacggcggcg gaagcctgtt tgtcggcgat
     6961 tttgaccgga cgttttttga aacccagcgc accaaaccct gccgcaaccg cgccggaaga
     7021 caccaagacc agctcgtgtc ccgcatgatg caatgcggca agctggcggg tgatggtttg
     7081 gattttgccg cgcgagagac tgccgtccga atgggtaatc gaagatgtgc cgactttaaa
     7141 tacgattctt ttgtatttca ttgtttccgt ccttgttggt ttgtcctgtc tcgttgccgc
     7201 cttgtgccgc cgaatttgcc ctgttctgcc gcaattgtca acaatcacgc cgcgtctgca
     7261 ataaaatgga caaaatgtat aaaattaata aaatctatgg cggtttattg agattttttc
     7321 aaatttatat tgccgttttg tccaaaatgc gtataatcct gtccatattt ctgctgtagg
     7381 ctgatttatt ttagacaagg actaccatgc aattagatat agaccgcttg gttgcttatt
     7441 tcggcggcgt gaacgcgctt gccgaagcgt tgaaacagca cgatcccgaa aatgccgcga
     7501 cgaccgccgc catctataaa tggcgcacgc gcgggtcgct gcctttggcg caactgcaaa
     7561 agctgaccgc gcttgccgaa tcgcaaggca ggccgctgga tttgaatgct tttttacaaa
     7621 aaaacgaatc tctggagaga acagaaatga cacaggccaa ccgcgttatc attttcgaca
     7681 ccaccctgcg cgacggcgag cagtcgcccg gcgctgctat gaccaaagag gaaaaaatcc
     7741 gcgtcgcccg ccagctggaa aaattgggtg tggacatcat cgaagcgggt tttgccgctg
     7801 ccagcccggg cgatttcgag gcggtcaatg cgattgcgaa aaccattacc aaatcaacgg
     7861 tctgttcatt gtcccgcgcc atcgagcggg acatccgtca ggcgggtgag gccgttgcgc
     7921 ccgcgccgaa aaaacgcatc cacaccttca tcgccaccag ccccatccat atggagtaca
     7981 aattgaagat gaagccgaag caggtgattg aggcggcggt caaagcggtg aaaatcgctc
     8041 gtgaatacac cgacgatgtg gaattttcct gcgaagacgc gttgcgttcg gaaatcgatt
     8101 tccttgccga aatctgcggc gcggtaattg aagcgggcgc gaccaccatc aatattcccg
     8161 ataccgtcgg ctattccatc ccgtacaaaa ccgaagaatt tttccgcgaa ctgattgcca
     8221 aaacgcccaa cggcggcaaa gtcgtttggt cggcacactg ccacaacgat ttgggcttgg
     8281 cggttgccaa ttcgcttgcc gcattaaaag gcggtgcgcg tcaggtggaa tgtactgtca
     8341 acggcttggg cgaacgtgca ggcaatgctt cggttgaaga aatcgtgatg gcgttgaaag
     8401 tgcgccacga cttgttcggc ttggaaaccg gcatcgatac cacgcaaatc gtgccttcgt
     8461 ccaaactggt gtccaccatt acgggctatc ccgtgcagcc caacaaagcc attgtcggtg
     8521 ccaatgcctt ttcgcatgaa tcgggcatcc atcaggacgg ggtgctgaaa caccgcgaaa
     8581 cttacgagat tatgtctgcc gaatcggtcg gttggtcggc taaccgattg agcttgggca
     8641 aattgtccgg ccgcaacgcc ttcaaaacca agctggcgga tttgggcatc gagttggaaa
     8701 gcgaagaggc actgaacgcg gcatttgcac gcttcaaaga actcgccgac aaaaaacgcg
     8761 aaatcttcga tgaagacctg cacgcgctgg tgtcggacga aatgggcaat atgaataccg
     8821 aaagctacaa attcatttcc caaaaaatca gcaccgaaac cggcgaagag ccgcgcgccg
     8881 acatcgtgtt cagcatcaaa ggcgaagaaa aacgcgcttc cgcaaccggt tccggtccgg
     8941 ttgatgcaat tttcaaagcg attgaaagcg tggcgcaaag cggcgcgact ttgcagattt
     9001 attccgtcaa tgccgtcacg caaggtacgg aaagccaagg cgaaaccagc gtccgtctgg
     9061 cacgcggcaa ccgcgtcgtc aacggtcagg gcgcggatac cgacgttttg gttgccaccg
     9121 ccaaagccta cctttccgcc ttgagcaaac tggaatttag tgccgccaaa ccgaaagcgc
     9181 aaggcagcgg tacgatttga gcgtgaaaac agacgatgcc gtctgaagca gaaaaaggct
     9241 tcagacggca ttgcggc
//
LOCUS       DAXHSF010000068         9255 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.068, whole genome shotgun sequence.
ACCESSION   DAXHSF010000068 DAXHSF010000000
VERSION     DAXHSF010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9255
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.068"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(<1..845)
                     /locus_tag="ACFM8S_001168"
     CDS             complement(<1..845)
                     /locus_tag="ACFM8S_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISNme5 family transposase"
                     /protein_id="HFC6314244.1"
                     /translation="MLIHYIDIAKRNFVIAVSSLSKTKTETNNPKGIAHTIEYLKKHK
                     VALVVTESTGGLEIPAAKAIRRAGIAVIIANPRQTHQFAQSQPLTKTDAKDAKMPAFF
                     AQMTAQKEDSQTMPYQPPTEAEEVLEALVNRRNQPADMRTAEKNRLHQVHETQVGSVK
                     QLIAHFDRLIDELDKQIDDHTHTHFDGKAQVAEQIKGIGSITTATLMAMLPELGRLSH
                     KRIASLVGIAPHPRESGETKFKSRCFGGRSAVRKALYMATVAATRFEPLIRDFYQRLL
                     SEGKP"
     gene            complement(1458..3038)
                     /gene="purH"
                     /locus_tag="ACFM8S_001169"
     CDS             complement(1458..3038)
                     /gene="purH"
                     /locus_tag="ACFM8S_001169"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFC6314245.1"
                     /translation="MPSIKRALISLSDKTGAVEFAQTLHKLGVEILSTGGTAKLLADA
                     GVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRRDLPEHVAKMEEHGIGNIDLVCV
                     NLYPFAATIAKPNCTLEDAIENIDIGGPTMVRSAAKNWKHVAIVTDTADFPAIAAELE
                     ANNGALSDKTRFNLSRKAFSHTAQYDGMISNYLTSLSDEVLSGTPEIGEFPSQFNQSW
                     IKVQDMRYGENPHQRAAFYRDIDPAAGSLSAYKQLQGKELSYNNIADADAAWEAVKSF
                     DAPACVIVKHANPCGVAVAADTLTAYKLAYVTDTTSAFGGIIAFNREVDGETVKQITD
                     NQFMEVLMAPKFTAEALEIAAAKKNVRVLEVPLEAGANRFELKRVGGGLLVQTPDIHR
                     LNRADLKVVSKRQPTEQEWNDLMFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMSRVDS
                     TRIAARKAQDAGLDLNGACAASDAFFPFRDGVDVIAEQGIKAIIHPAGSMRDQEVFDA
                     ADEHGIAMVVTDVRHFRH"
     gene            complement(3202..4425)
                     /locus_tag="ACFM8S_001170"
     CDS             complement(3202..4425)
                     /locus_tag="ACFM8S_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFC6314246.1"
                     /translation="MGRRKLWFALAAAGVIGGLVGIVLTELMHFIQHTAYGYGADGAY
                     ISFREGVAQASGMRRVAMLTLCGAIAGGGWWLLKALGKPQIEIKAALKQPLQGLPFLT
                     TVFHVLLQIITVGLGSPLGREVAPREMTAAFASAGGKRLGLDEGEMRLLIACASGAGL
                     AAVYNVPLASTLFILEAMLGMWTQQAVAAALLTSVIATAVARIGLGDVQQYHPANLAV
                     NTSLLWFSAVIGPILGATAVFFQSTAQKFPFIKRDNIKIIPLAVCMFALIGAIAVWFP
                     EVLGNGKAGNQLTFGGLTDWQHSLELTAVKWLVVLMALAAGAYGGLITPSMMLGSTIS
                     FAAAAAWNSVFPEMPSESAAVVGAAVFLGVSLNMPLTAAVFVLELTYAPAALLMPLCT
                     GMAGAVWTARKMGCK"
     gene            complement(4489..5322)
                     /gene="serB"
                     /locus_tag="ACFM8S_001171"
     CDS             complement(4489..5322)
                     /gene="serB"
                     /locus_tag="ACFM8S_001171"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HFC6314247.1"
                     /translation="MPHALVLQFPSAEALPSDFPSRLPEPDYADEKRMRFIVEEGFSL
                     SEADAALLGSRQIDHAVLPDMDFDELGLIVSDMDSTLITIECVDEIAAGVGLKDKVAE
                     ITERSMRGELDFEQSLRSRVALLAGLDEQILADVYENVLKLSPGAEFLLDECKRHDVK
                     FLLVSGGFTFFTERLQQRLGFEYQHANVLEIENGRLTGRLKGRIIDAQAKADLLREYR
                     SRLGLQPHQVLAMGDGANDIPMLKEAGIGVAYRAKPKAQAAADACINFGGLERVRGLF
                     R"
     gene            5612..7153
                     /locus_tag="ACFM8S_001172"
     CDS             5612..7153
                     /locus_tag="ACFM8S_001172"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFC6314248.1"
                     /translation="MKIGIPRESLSGETRVACTPATVALLGKLGFETVVESGAGLAAS
                     LDDAAYQTAGATVADKAAVWACPLIYKVNAPSEGELPLLNEGQTIVSFLWPRQNEALV
                     EALRAKKVNALAMDMVPRISRAQALDALSSMANISGYRAVIEAANAFGRFFTGQITAA
                     GKVPPAQVLVIGAGVAGLAAIGTANSLGAVVRAFDTRLEVAEQIESMGGKFLKLDFPQ
                     ESGGSGDGYAKVMSDEFIAAEMKLFAEQAKEVDIIITTAAIPGKPAPKLITKEMVESM
                     KSGSVIVDLAAATGGNCELTRPGELSVTGNGVKIIGYTDMANRLAGQSSQLYATNLVN
                     LTKLLSPNKDGEITLDFEDVIIRNMTVTHDGEITFPPPPIQVSAQPQQTPSEKAAPAA
                     KPEPKPVSLWKKLAPAVIAAVLVLWVGAVAPAAFLNHFIVFVLACVIGYYVVWNVSHS
                     LHTPLMSVTNAISGIIVVGALLQISQGNGFVTLLAFIAILIASINIFGGFFVTRRMLN
                     MFRKG"
     gene            7157..7540
                     /locus_tag="ACFM8S_001173"
     CDS             7157..7540
                     /locus_tag="ACFM8S_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFC6314249.1"
                     /translation="MTFAYWCILIACLLPLFCAAYAKKAGGFRFKDNHNPRDFLARTQ
                     GAAARAHAAQQNSFEAFAPFAAAVLTAHATGNAGQATVNTLAGLFILFRLAFIWCYIA
                     DKAALRSLMWAGGFACTVGLFVAAA"
     gene            7606..8991
                     /gene="pntB"
                     /locus_tag="ACFM8S_001174"
     CDS             7606..8991
                     /gene="pntB"
                     /locus_tag="ACFM8S_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFC6314250.1"
                     /translation="MSSGLVTAAYIVAAILFIFSLAGLSKQETAKQGCYSGIAGMAVA
                     LFVTVFSDNTHGLGWIIIAMLIGAAIGIYKAKKVEMTEMPELIALLHSFVGLAAVLVG
                     FNSYIEPGNVSHDMHTIHLVEVYLGIFIGAVTFTGSLVAFGKLNGKISSSPLQLPAKH
                     KLNALALAVSFVLLLVFVGINGSGFILLIMTLIALAFGWHLVASIGGADMPVVVSMLN
                     SYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGSDSG
                     TLSSGSQEIGEYREVKAADIAEMLKGANNVIITPGYGMAVAQAQYPVAEITELLRKNG
                     TEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFPETDVVLVIGANDTVN
                     PAAQTDPNSPIAGMPVLEVWKAKEVVVFKRSMNTGYAGVQNPLFFNENSVMCFGDAKK
                     TVDDILSELKK"
BASE COUNT         2014 a         2580 c         2550 g         2111 t
ORIGIN      
        1 tacggcttac cctcggacag caggcgttgg tagaaatccc gaataagcgg ttcaaaacgt
       61 gtcgctgcca cggtagccat atacagtgcc ttacgcaccg cagaccttcc gccaaagcag
      121 cggcttttga atttggtttc cccgctctcc ctcgggtgcg gggcaatgcc gaccaaactc
      181 gctatccgtt tgtgcgacag ccgccccaac tcaggcagca tcgccatcag cgtagccgtc
      241 gttatcgaac cgatgccttt gatttgttct gccacttggg ctttgccgtc aaaatgcgtg
      301 tgggtgtggt cgtcgatttg tttgtccaat tcgtcaatca gccggtcaaa atgggcaatc
      361 agttgtttga cgcttccgac ttgcgtttcg tgaacctgat gcagacggtt tttctcggca
      421 gtccgcatat ccgccggttg gttgcggcgg ttaaccaagg cttccaacac ttcttccgct
      481 tcggtgggcg gttggtaggg catggtttgc gaatcttctt tctgtgccgt catctgtgcg
      541 aagaaggcgg gcattttggc atctttggcg tcggttttgg tcagcggctg cgattgggca
      601 aactgatgcg tctgacgcgg gttggcgata atcacggcta tgcctgctcg gcggatggct
      661 ttggcggcgg ggatttcgag accgccagta ctttccgtca cgacgagggc gaccttgtgt
      721 tttttaaggt attcgatagt atgggcgata cctttggggt tgttggtttc ggttttggtt
      781 ttagacaaag acgaaacggc gatgacgaag tttcgtttgg cgatgtcgat atagtgaatt
      841 aacaaaaatc aggacaaggc ggcgagccgc agacagtaca aatagtacga aaccgattca
      901 cttggtgctt cagcacctta gagaatcgtt ctctttgagc taaggcgaac caacgctgta
      961 ctggtttaga tttaattcac tatacctgcg taatggtatt gggtactcat cataaacctg
     1021 ccttgcattc ggttgtgttg tccggcaact gtccggttgt gtcgatgggt tgccctgccg
     1081 ctccctgagc tacgcaacgg ttgtgtgcct tgggtgggcg cgggtggcgg ccgggcggtt
     1141 tgttgctatg atacagtgat tccaatatac aagggtgggc ttcagcccac cgcttccgtc
     1201 gattccgtca atgttaccca tttccaccgt ccccgccgaa accaaaaccg ccgactcccg
     1261 ccggttctcc aaaaattttt gatgcagcgg gctgaagcac accctgcatc ccacctttta
     1321 cgaatcctcc tacaccctat acaacacctt gaaaatccac cctgtcagga atacccgaac
     1381 cgtcatccct accttcacaa aatagcgcaa aatgccgtct gaaagccctt cagacggcat
     1441 tgccttgttc atctgcgtta atggcggaag tggcgcacgt cggttacgac catggcgatg
     1501 ccgtgttcgt ccgctgcgtc gaagacttcc tgatcgcgca tcgaacctgc cggatggatg
     1561 atggctttga tgccctgctc ggcaatcacg tccacgccgt cgcggaaggg gaagaaagca
     1621 tcagaggcgg cacatgcgcc gtttaagtcc aagcctgcgt cctgcgcttt gcgggcggcg
     1681 atgcgggtac tgtccacgcg gctcatttgg cctgcgccga tgccgtaggt ttgaccgcct
     1741 ttgccgaaga cgatggcgtt ggatttgacg tattttgcga cgttccaaac aaacatcagg
     1801 tcgttccatt cttgctcggt cggttggcgt ttggaaacga ctttcaaatc ggcgcggttg
     1861 aggcggtgaa tatcgggggt ttgcaccaac agtccgccgc cgacgcgttt gagttcgaag
     1921 cggtttgcgc ctgcctcaag cggcacttcc aatacgcgca cgtttttctt ggcggcggcg
     1981 atttcgaggg cttcggcggt aaacttcggc gccatcaaga cttccataaa ctggttgtcg
     2041 gtaatctgtt tgacggtttc gccgtccact tcgcggttga aggcgatgat gccgccgaac
     2101 gcgctggtgg tgtcggtgac gtaggcgagt ttgtaggcgg tcaatgtatc ggctgcaacg
     2161 gcgacgccgc acggattggc gtgtttgacg atcacgcagg ctggcgcgtc aaaggatttg
     2221 acggcttccc atgcggcatc ggcatcggcg atgttgttgt aagacaattc tttgccttgc
     2281 agttgtttgt aggcggaaag gctgcctgcg gcggggtcaa tatcgcggta gaacgcggcg
     2341 cgctgatgcg ggttttcgcc gtagcgcatg tcttgcactt taatccagct ttggttgaac
     2401 tggctcggga attcaccgat ttcgggcgtg ccgctcaaga cctcgtctga aagcgaggtc
     2461 aggtagttgg aaatcatgcc gtcgtattgg gcggtgtggc tgaacgcttt gcgcgacagg
     2521 ttgaagcggg ttttgtcgct caacgcgccg ttgttggctt cgagttcggc ggcgatggcg
     2581 gggaagtcgg cggtgtcggt aacgatggcg acgtgtttcc agttttttgc ggcagaacgc
     2641 accatggtcg ggccgccgat gtcgatgttt tcaatcgcgt cttccagtgt gcagtttggt
     2701 ttggcgatgg tggcggcgaa gggataaagg ttgacgcaca cgaggtcgat gttgccgatg
     2761 ccgtgttctt ccatcttggc gacgtgttca ggcaaatcgc gccggcccaa aataccgccg
     2821 tggattttcg gatgcagcgt tttcacgcgg ccgtcgagca tttcggggaa accggtgtag
     2881 tcggcaactt caatcacggg aacgcccgca tcggcaagca gcttcgccgt gccgccggta
     2941 gagaggattt cgacaccgag cttgtgcagg gtttgggcaa attcgactgc gcccgtcttg
     3001 tcggacaggc tgatcagggc gcgtttgatg gaaggcatag ggatttcctt tttctgacgt
     3061 tgggatggga aacggtaatg ttgtcggggc ggtattatcc gctattttcc actttccggc
     3121 agtaggtttt tgcagatatt gttgacaatc cccaaaggaa tgccgtctga aaaccggttt
     3181 cagacggcat tttgccttcg gttatttaca tcccattttc cttgccgtcc ataccgcgcc
     3241 cgccataccc gtacataaag gcatcaacaa agcggcgggg gcgtaggtga gttccaaaac
     3301 aaagacggcg gcggtcagag gcatattgag ggaaacgccg aggaaaacgg cggcgccgac
     3361 gactgccgcg ctttcagacg gcatttcggg gaaaacgctg ttccacgcgg cggcggctgc
     3421 aaaagaaatg gtgctgccga gcatcataga cggcgtaatc agtccgccgt atgcgcctgc
     3481 cgccagcgcc atcaagacga ccagccattt gacggcggtc aactcaaggc tgtgttgcca
     3541 gtcggtcaaa ccgccaaaag tcagttggtt gcctgctttg ccgttgccca aaacttcggg
     3601 aaaccaaacg gcaatcgcgc cgatgagtgc aaacatacag acggccaagg gaatgatttt
     3661 gatgttgtcg cgcttgatga aggggaattt ttgggcggta ctctggaaaa aaacggcggt
     3721 tgcgcccagt atcgggccga tgacggcgga aaaccaaagt aatgaggtgt tgacggcaag
     3781 gttggcggga tgatattgct gcacgtcgcc taagccgatg cgtgcgacgg cggtggcaat
     3841 gaccgaagtc agcaacgcgg cggcgacggc ttgctgcgtc cacatgccca gcatggcttc
     3901 gaggatgaaa agtgtggagg cgagcggcac gttatacacg gccgccaaac ccgcgccgga
     3961 cgcacaggca atcaagaggc gcatttcgcc ttcgtccaaa cccaagcgtt tgccgcctgc
     4021 cgaagcaaac gcggcggtca tttcgcgcgg cgcgacttcg cgtccgagcg gtgaaccgag
     4081 tccgaccgtt atgatttgca gcagaacatg gaaaaccgtc gtcagaaacg gcagcccctg
     4141 caacggctgc ttcaaggcgg ctttgatttc gatttgcggc ttgcccaagg ctttcagcaa
     4201 ccaccagccg ccgcctgcga tcgcgccgca cagcgtcagc atggcgacgc gccgcatacc
     4261 ggaagcctgt gccacacctt cgcggaacga gatgtacgca ccgtccgcgc cataaccgta
     4321 tgccgtatgc tgtatgaagt gcatcagttc tgtcagcaca atgccgacta aaccgccgat
     4381 aacgcccgct gctgccaggg caaaccaaag ttttctgcgt cccactgtcg ttcctgacgt
     4441 tcaaatgccg tctgaaaacc tttcagacgg catccgtttc ctatccgcct acctgaacag
     4501 gccgcgtaca cgctccaaac cgccgaagtt gatacaggca tcggcggctg cctgcgcttt
     4561 cggtttggca cggtaagcca cgcctatgcc cgcttctttg agcatcggaa tatcgttcgc
     4621 accgtcgccc atcgccaaca cctgatgcgg ctgcaatccg aggcggctgc ggtattcgcg
     4681 caataaatcc gcctttgcct gcgcgtcgat gattctgcct ttcagacgac cggtcaacct
     4741 gccgttttca atttccaaaa cattggcgtg ttggtattcg aagccgagtc gttgttgcag
     4801 cctttcggta aaaaatgtga acccgccgga caccagcagg aatttcacat cgtgcctttt
     4861 gcattcgtcc aacaagaatt ccgcaccggg cgagagcttc aaaacgtttt cataaacgtc
     4921 cgccaaaatc tgttcgtcca atcccgccaa cagcgcaacg cggctgcgta aagactgttc
     4981 gaaatcgagt tcgccgcgca tcgaacgctc ggtaatttcc gctactttgt cttttaaacc
     5041 cacgcctgcc gcaatttcat cgacgcattc aatggtaatc agcgtcgaat ccatatcgct
     5101 gacaattaaa ccgagttcgt cgaaatccat atccggcaac acggcgtggt cgatttgacg
     5161 gctgccaagc aacgccgcgt ccgcctcgct taaagaaaac ccttcttcaa cgataaaacg
     5221 catacgcttt tcatcggcgt aatcaggttc gggcaggcgt gaggggaagt cggaaggcag
     5281 ggcttcggcg gagggaaatt ggaggacgag ggcgtgcggc atggcggtaa ctcaaaaaat
     5341 gcggattata tagtgaaacg gcaaagaatg ttacggcggg cgggactgca atcggaaaat
     5401 gacttcaaac gcaaacggca gtatgtgtcg gacaacacgg gaaaatgccg caactattgc
     5461 cagcctgatg aaaattcgtt ataaggggat tatctaaaat atattaacat ttgaagtgaa
     5521 tcggttttaa accggtacgg cgttgctccg ccctgccccg atttaaattt aacccacgat
     5581 acatataaac tacccgaaaa aggattcaga gatgaaaatc ggtatcccac gcgagtcatt
     5641 atccggcgaa acccgcgtcg cctgcacgcc cgccaccgtc gccctgctgg gcaaactggg
     5701 ctttgaaacc gttgtcgaaa gcggtgcagg tttggcggca agtttggacg atgccgctta
     5761 ccaaacagca ggcgcgaccg ttgccgacaa agcggcggtt tgggcctgcc ctttgattta
     5821 taaggtcaat gcgccgtccg aaggcgagct gccgcttttg aacgaaggtc aaaccatcgt
     5881 cagcttcctg tggccgcgcc aaaacgaggc tttggtcgaa gccttgcgcg ccaagaaagt
     5941 gaacgcgctg gcgatggaca tggtgccccg catttcgcgc gcgcaggctt tggacgcttt
     6001 gtcttcgatg gcaaacatca gcggctaccg cgccgtaatt gaagccgcca acgccttcgg
     6061 ccgtttcttc accggccaaa tcaccgccgc cggcaaagtg ccgcccgcgc aggttttggt
     6121 gattggcgca ggtgtggcag gtttggcggc gatcggtacg gcaaactcgc tcggcgcagt
     6181 ggtgcgcgcg ttcgataccc gcttggaagt ggcggaacaa atcgaatcga tgggcggcaa
     6241 gttcctgaaa ctcgacttcc cacaagaatc gggcggcagc ggagacggct acgccaaagt
     6301 gatgagcgac gaatttatcg cagccgagat gaagctcttt gccgagcagg cgaaagaagt
     6361 ggacatcatc atcaccaccg ccgccattcc gggcaaaccc gcgcccaagc tgattaccaa
     6421 agaaatggtg gaaagcatga aatccggctc cgtcatcgtc gatttggcgg cggcgacggg
     6481 cggcaactgc gaactcaccc gcccgggcga attgtccgta accggcaacg gcgtgaaaat
     6541 catcggctac accgacatgg caaaccgcct tgccggacag tcttcccagc tttacgccac
     6601 caacttggtc aacctgacca agctgttaag cccgaacaaa gacggcgaaa tcacgttgga
     6661 cttcgaagac gtgattatcc gcaacatgac cgttacccac gacggcgaaa tcaccttccc
     6721 gcctccgccg attcaagttt ccgcccagcc gcagcaaacg ccgtctgaaa aagccgcgcc
     6781 tgccgccaag cccgagccga aacctgtttc cctgtggaaa aaactcgcgc ccgccgtcat
     6841 cgccgccgta ttggtgctgt gggtcggcgc ggtcgcaccc gcagcattcc tgaaccactt
     6901 tatcgtgttc gtccttgcct gcgtcatcgg ctactatgtc gtttggaacg tcagccactc
     6961 gctgcacaca ccgctgatgt cggtaaccaa cgccatctcc ggcatcatcg tcgtcggcgc
     7021 gctgctgcaa atcagccaag gcaacggttt cgttaccctg cttgccttca tcgccatcct
     7081 gattgccagc atcaatatct tcggcggctt cttcgtaacg cgccgcatgt tgaatatgtt
     7141 taggaaagga taaagcatga cttttgccta ttggtgcatc ctgattgcct gcttattgcc
     7201 gcttttttgt gcggcatatg ccaaaaaagc gggcggattc cggtttaaag acaaccacaa
     7261 tccgcgcgat tttctggcgc gtacccaagg tgccgctgcc cgtgcccatg ccgcgcagca
     7321 aaacagtttt gaagcatttg caccatttgc agccgccgtt ttgacggcac acgcaaccgg
     7381 caatgccgga caagcaaccg tcaacacgct tgccggcctg ttcatcctgt tccgcctcgc
     7441 ctttatctgg tgctacatcg cagacaaagc agcattgcgc tcgctgatgt gggcgggcgg
     7501 atttgcctgc accgtcgggc tgtttgtcgc ggctgcttga aacagatgcc gtctgaacag
     7561 ttcagacggc attaaaaaca aatcatcgaa aatcggagaa tttccatgtc ttcaggactc
     7621 gtaacagcgg cgtatatcgt tgccgcaatt ttattcatct tctcactggc ggggctgtcc
     7681 aaacaggaaa ccgccaaaca gggctgctat tccggtatcg ccggtatggc ggtcgccctt
     7741 tttgtaactg ttttttccga caatacccac ggactgggct ggatcatcat cgccatgctc
     7801 atcggcgcgg caatcggcat ctacaaagcc aaaaaggtgg aaatgaccga aatgcccgaa
     7861 ctgattgccc tgctgcacag cttcgtcggc ctagcggcgg ttttggtcgg cttcaacagc
     7921 tatatcgagc cgggcaacgt ttcgcacgat atgcacacca tccatctggt cgaagtctat
     7981 ttgggcattt tcatcggcgc ggtaaccttt accggctcgc tggtcgcatt cggcaaactc
     8041 aacggcaaaa tcagcagcag cccgctgcaa ctgcccgcca aacacaagct caacgcactg
     8101 gcgctcgccg tatcgtttgt gttgctgctc gtattcgtcg gcattaacgg tagcggcttc
     8161 atcctgctga ttatgaccct gattgccctc gcattcggct ggcacttggt tgcctccatc
     8221 ggcggcgcag atatgcccgt ggtcgtgtcc atgctcaact cctactccgg ctgggcggcc
     8281 gcagcggcag gcttcatgct ctccaacgac ctgctcatcg ttaccggcgc gctggtcggc
     8341 tcaagcggcg cgattctgtc ctacattatg tgcaaagcca tgaaccgctc gtttgtctcg
     8401 gtaattgccg gcggtttcgg cagcgacagc ggcacattat cttccggcag ccaagagata
     8461 ggggaatacc gagaagtcaa agctgccgat attgccgaaa tgctgaaagg cgcaaacaat
     8521 gtcatcatta ccccgggcta cggtatggca gtcgcacaag cgcaataccc cgttgccgaa
     8581 atcaccgagc ttttacgtaa aaacggcacc gaagtacgct tcggcatcca ccccgtcgcc
     8641 ggccgcctgc ccggtcatat gaacgtactg ctcgccgaag ccaaagtccc ctacgacatc
     8701 gttttggaaa tggacgaaat caacgacgac ttccccgaaa ccgatgtggt cttggtcatc
     8761 ggtgcgaacg acaccgtcaa ccccgccgcc caaaccgacc cgaacagccc gattgcgggt
     8821 atgcccgtgt tggaagtgtg gaaggcaaaa gaagtcgtcg tcttcaaacg ctcgatgaat
     8881 accggctacg caggtgtaca aaacccactg ttcttcaacg aaaacagcgt gatgtgtttc
     8941 ggagatgcga agaaaaccgt ggatgacatt ctgtccgaac tgaaaaaata atgccgtctg
     9001 aacaattcgg cgcaggtttt caatctgttt gatttgaaaa aatcactgca aaccccgttt
     9061 atgaaggttt gcagtgattt ttttgcattg gggcaaacat tttcagacgg cccgtccgaa
     9121 taagcaccta catctgaaac tgacacaaaa tcaacgaaac aaacaaaatc catctccgtg
     9181 ttgaagatgg atttttaatt agccgaacac tttttccaag tgtgcctgat aatctgccaa
     9241 gtatttttcc acttg
//
LOCUS       DAXHSF010000069         9199 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.069, whole genome shotgun sequence.
ACCESSION   DAXHSF010000069 DAXHSF010000000
VERSION     DAXHSF010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9199
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.069"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            89..298
                     /locus_tag="ACFM8S_001175"
     CDS             89..298
                     /locus_tag="ACFM8S_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HFC6314251.1"
                     /translation="MPYVNIKVTGGKEAPTAAQKAELIGGVTELLARVLGKNPETTVV
                     VIDEVDTDNWGIGGKSVSERRKEGR"
     gene            387..1601
                     /locus_tag="ACFM8S_001176"
     CDS             387..1601
                     /locus_tag="ACFM8S_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFC6314252.1"
                     /translation="MDKFPKSAKLDHVCYDIRGPVHKKALQLEEEGNKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTSQGYCDSKGLYSARKAIVHYYQTKGLRDITVNDVYIGN
                     GVSELITMSMQALLNDGDEILIPAPDYPLWTAAATLAGGTVRHYLCDEENGWFPNLAD
                     MEAKITPKTKAIVVINPNNPTGAVYSREILLEIAELARKHGLIIFADEIYDKILYDGA
                     VHHHIAALAPDLLTVTFNGLSKAYRVAGFRQGWMVLNGPKHHAKGYIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFILPGGRLLEQRNRAWELVNQIPGVSCVKPMGAMYM
                     FPKIDTEMYRIHDDMKFVYDLLVREKVLLVQGTGFNWIKPDHFRIVTLPYVHQIEEAM
                     GRLARFLQTYRQ"
     gene            complement(1688..4267)
                     /gene="clpB"
                     /locus_tag="ACFM8S_001177"
     CDS             complement(1688..4267)
                     /gene="clpB"
                     /locus_tag="ACFM8S_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFC6314253.1"
                     /translation="MRYDKLTAKFQQALAEAQSLALAADGSYLEAGFVLKALLDDQNS
                     GAAALLAHSGVNVPQVKQRLQQHLNSLPKVSSQGGDILPSRELQAVLNLMDKAATKRG
                     DAYIASELFLLALVQQNDATGKILKEAGATEQNINAAIDAVRGGQNVNDANAEDQRDA
                     LKKYTLDLTQRARDGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLRNKRLLVLDLAALIAGAKYRGEFEERLKGVLNDLAKDDGNTLIF
                     IDEIHTLVGAGKTDGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVGEPSVEDTIAILRGLQERYEIHHGIDITDPAIVAAAELSDRYITDRFLPDKAID
                     LIDEAASRVKMEKETKPEAMDKIDRRLIQLRMEKAHVEKEKDDASKKRLELIDEEING
                     LQKEYADLDEIWKAEKAISDGAANIKKQIDEVKIKIEQAKRQGDLALASKLMYEDLEH
                     LEKQRAAAERADTDSTKPANKLLRNNVGAEEIAEVVSRMTGIPVSKMMEGERDKLLKM
                     EEVLHRRVVGQDEAVRAVSDAIRRSRSGLADPNKPYGSFLFLGPTGVGKTELCKALAG
                     FLFDSEDHLIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILLDE
                     VEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSQHIQQMGIQDYEAV
                     KEVVMEDVKEHFRPEMINRIDEVVVFHGLDQDNIRNIAKIQLKGLEKRLEKQNLRLTV
                     SDAALDIIAKAGFDPIYGARPLKRAIQSEIENPLAKALLVGNYAPESEIRVEADGDRL
                     KFA"
     gene            4528..5538
                     /gene="trpS"
                     /locus_tag="ACFM8S_001178"
     CDS             4528..5538
                     /gene="trpS"
                     /locus_tag="ACFM8S_001178"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFC6314254.1"
                     /translation="MSKKRVLTGVTTTGIPHLGNYVGAIRPAVRAAQNPDTESFLFLA
                     DYHGIIKCHEPEMIHQSTQAVAATWLACGLDPERTTFYRQSDIPEVMELNWILTCITA
                     KGLMNRAHAYKAAVQANAENGQEDPDFGVEMGLFSYPILMTADILMFNANEVPVGRDQ
                     IQHVEMARDIAGRFNHRFKEIFTLPEVKIDENVELLVGLDGRKMSKSYGNTIPLWEND
                     KKTQKSVNKIITNMKEPGEPKQPDESPLFEIYKAFSTPSETAAFTQMLAEGLAWGEAK
                     KLLAAKINAELAEPRERYNELTADPSQIEEILQAGAQKARKEARELLDKVRDAVGIRP
                     LK"
     gene            5544..5885
                     /locus_tag="ACFM8S_001179"
     CDS             5544..5885
                     /locus_tag="ACFM8S_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314255.1"
                     /translation="MPSEPLKGLPEQNVVRLTGKHPGDLEAVGGKCMETDGKGAPSGW
                     AANGVCHTLFAKLVGNIAEDGGKLTDYLISHSALQPYQAGKSGYAAVQNGRYVLEIDS
                     EGAFYFRRRHY"
     gene            6204..6527
                     /locus_tag="ACFM8S_001180"
     CDS             6204..6527
                     /locus_tag="ACFM8S_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314256.1"
                     /translation="MKKLLIAAMMAAALAACSQEAKQEVKEAVQAVESDVKDTAASAA
                     ESAASAVEEAKDQVKDAAADAKASAEEAVTEAKEAVTEAAKDTLNKAADATQEAADKM
                     KDAAK"
     gene            complement(6736..8244)
                     /gene="ppx"
                     /locus_tag="ACFM8S_001181"
     CDS             complement(6736..8244)
                     /gene="ppx"
                     /locus_tag="ACFM8S_001181"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFC6314257.1"
                     /translation="MTTHPANVLASVDLGSNSFRLQICENNNGQLKVIDSFKQMVRFA
                     AGLDEQKNLSAASQEQALDCLAKFGERLRGFRPEQVRAVATNTFRVAKNIADFLPKAE
                     AALGFPIEIIAGREEARLIYTGVIHTLPPGGGKMLVIDIGGGSTEFVIGSTLNPDITE
                     SLPLGCVTYSLRFFQNKITAKDFQAAISAARNEIQRISKNMRREGWDFAVGTSGSAKS
                     IRDVLAAEMPQEADITYKGMRALAERIIEAGSVKKAKFENLKPERIEVFAGGLAVMMA
                     AFEEMKLDRMTVTEAALRDGVFYDLIGRGLNEDMRGQTVAEFQHRYHVSLNQAKRTAE
                     TAQTFMDSLCHAKNVTVQELALWQQYLGRAAALHEIGLDIAHTGYHKHSAYILENADM
                     PGFSRKEQTILAQLVIGHRGDMKKMSGIIGTNEMLWYAVLSLRLAALFCRSRQDLSFP
                     KNMQLRTDTESCGFILRIDREWLERHPLIADALEYESVQWQKINMPFKVEAV"
     gene            complement(8361..9092)
                     /locus_tag="ACFM8S_001182"
     CDS             complement(8361..9092)
                     /locus_tag="ACFM8S_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC6314258.1"
                     /translation="MSREINAGGHHFLYGGISGGGVHYWDNKDFSEQCLRLSLGYKNR
                     SVTRSFGIVPFVGQNLLGGSRYNFVGGFNADFSQRLGEHWRLTLNAGNMWKRYQEDRT
                     AARYDSHMPLAGATLMYSAPKDWLLYGGADWSHDLTKEAEQASVRKGLRIGAVKTFDG
                     GLGLRANLRYTRRMFDAPGTIVYRFPRKDHEYQANLSLWHDKISWKGFTPQLNFRYLK
                     IDSNMKSFYTRKNTQIFMSVEKDFK"
BASE COUNT         2120 a         2396 c         2428 g         2255 t
ORIGIN      
        1 gatttgaatt tttcagaaaa tgaagcggac gtttgggcgg aggcaacttg tttgataaga
       61 tagcaatatt tcaaaacgga gaaatatcat gccttatgtc aatattaaag taaccggcgg
      121 caaggaagca ccgactgccg cgcaaaaagc ggaactgatc ggcggcgtaa ccgaattgct
      181 ggcacgcgtg ctgggcaaaa atcccgaaac aacggttgtc gtgattgacg aagtggatac
      241 cgataactgg ggaataggcg gcaaaagcgt cagcgaacgg cgcaaagagg gcaggtaaaa
      301 agcctgaaaa tctcggtttg atgctttaaa ttccgcgtga aaaagagtac attcccaccc
      361 attgcccaaa atttacggaa cacattatgg ataaatttcc caagtctgca aagctcgatc
      421 acgtctgtta cgacatacgc gggccggttc acaaaaaagc ccttcagttg gaagaggagg
      481 gcaataaaat ccttaaactc aatatcggca accctgcgcc gttcggcttt gaagcccctg
      541 atgaaatctt ggtcgatgtc atccgcaacc tgccgacttc gcaaggctat tgcgattcta
      601 aagggctgta ttccgcccgc aaagccattg ttcactacta tcagaccaaa ggtttgcgcg
      661 atattacggt aaacgatgtc tatatcggca acggcgtgtc cgagctgatt acgatgtcta
      721 tgcaggcatt gctcaacgac ggcgacgaaa tcctgattcc cgcgcccgac taccctttgt
      781 ggacggcggc ggcaacgctt gcgggcggta cggtacgcca ttatctgtgc gacgaagaaa
      841 acggctggtt ccccaacctt gccgatatgg aagccaaaat cacgcccaaa accaaagcca
      901 tcgtcgtcat caatcccaat aatccgacag gcgcggtgta cagcagggaa atcctgttgg
      961 aaatcgccga actggcgcgc aagcacggtt tgattatttt cgccgacgag atttacgata
     1021 aaatccttta tgacggcgcg gttcaccacc atattgccgc gcttgccccc gaccttttga
     1081 cggtaacgtt caacggtttg tccaaagcct accgcgtcgc cggcttccgc caaggctgga
     1141 tggtgctcaa cgggccgaaa catcacgcaa aaggttacat cgagggtttg gatatgctct
     1201 cgtccatgcg cttgtgtgcg aatacgccga tgcagcatgc gattcagacg gcattgggcg
     1261 gttatcagag cattaacgaa ttcattttgc ccggcggacg gcttttggaa cagcgtaaca
     1321 gggcgtggga attggtcaat cagatacccg gcgtatcctg cgtcaaaccg atgggcgcga
     1381 tgtatatgtt cccaaaaatc gataccgaaa tgtaccgtat ccacgatgac atgaaattcg
     1441 tttacgattt gctggtgcgc gaaaaagtct tgctggtgca gggaacgggg tttaattgga
     1501 tcaagcccga ccatttccgc attgtcacgc tgccttacgt ccatcagatt gaagaggcga
     1561 tgggcaggct ggcaagattc ctgcaaacct accgccaata aggggacggt ttgtctgccg
     1621 aggataaaaa atgccgtctg aaacggagat tcccgtttca gacggcattt tcaacagcag
     1681 gaacgaatca ggcaaatttc agtctgtcgc cgtcggcttc caccctgatt tcgctttcgg
     1741 gcgcatagtt tccgacaagc agggcttttg ccagcgggtt ttcgatttcc gactggatgg
     1801 cgcgtttgag cggacgtgcg ccgtaaatcg ggtcgaaacc ggctttggcg atgatgtcca
     1861 gagcggcatc ggaaacagtc aggcggaggt tttgtttttc caaacgtttt tccaagcctt
     1921 tgagctggat tttcgcaatg ttgcggatat tatcctgatc cagtccgtgg aacacgacca
     1981 cttcgtcgat gcggttgatc atttcaggac ggaaatgttc tttcacatcc tccatcacaa
     2041 cttcttttac cgcttcgtaa tcctgaatgc ccatttgttg gatgtgctgg ctgccgatgt
     2101 tggaagtcat cacaatcacg gtatttttaa aatctaccgt cctgccttgc ccgtcggtca
     2161 aacgaccgtc atccaatact tgcagcagga tgttgaacac gtcgggatgg gctttttcca
     2221 cttcgtccaa aagaatcacg ctgtacggtt tgcggcggac ttgttcggtc aggtaaccgc
     2281 cctcttcgta gccgacatag cccggaggcg cgccgatgag gcgggcaacg gcgtgttttt
     2341 ccatatattc ggacatatcg atacgaatca gatgatcttc actgtcaaac aggaagcctg
     2401 ccaatgcctt acacaactcg gttttaccca cgccggtcgg gcctaggaac aggaagctgc
     2461 cgtagggttt attcgggtcg gcaagaccgg aacggctgcg gcggatagcg tcggacacgg
     2521 cacgcacggc ttcgtcttga ccgactacgc ggcggtgcaa tacttcttcc attttcagca
     2581 gtttgtcgcg ttcgccttcc atcattttgg atacgggaat gccggtcata cgggaaacca
     2641 cctctgcgat ttcctctgcg ccgacattat tacgcaagag tttgtttgcc ggttttgtgc
     2701 tgtccgtatc tgcccgttcg gcggctgcac gctgtttttc caaatgctcc aaatcttcat
     2761 acatcaattt tgaagccagt gccaaatcgc cttgccgttt tgcctgttcg attttaattt
     2821 tgacttcgtc aatttgtttc ttaatattag cagcaccgtc tgaaattgct ttttcggctt
     2881 tccagatttc gtctaaatcg gcgtattctt tttgcagacc gttgatttcc tcgtctatca
     2941 gttccaaacg ttttttgctg gcatcgtctt tttctttttc aacgtgcgcc ttttccatcc
     3001 gaagctgaat tagacggcgg tcgattttgt ccattgcttc cggcttggtt tctttttcca
     3061 tcttgacacg gctggcggct tcgtcaatca aatcaatcgc tttatcaggc aggaagcggt
     3121 cggtaatgta gcggtcgctc aactccgctg cggcaacgat ggcggggtcg gtgatgtcga
     3181 tgccgtggtg gatttcgtag cgttcctgca aaccgcgcaa gatggcgatg gtgtcttcca
     3241 cgcttggctc gccgaccaat actttttgga agcggcgttc gagtgccgcg tctttttcga
     3301 tgtattgacg gtattcgtcc aaggtggtcg cgccgataca gtgcaattcg ccacgtgcca
     3361 aagccggttt cagcatattg cccgcgtcca tcgcgccgtc ggttttgccc gcgccgacca
     3421 aagtatggat ttcatcaatg aaaatcaggg tgttgccgtc gtctttcgcc aaatcgttca
     3481 acacgccttt caagcgttct tcaaattcgc cacggtattt cgcaccggca atcaaagccg
     3541 ccaagtccag aaccaaaagg cgtttgttac gcagggattc aggtacttcg ccattgacga
     3601 tacgttgcgc caagccttca acaatagcgg ttttacccac acccggctca ccaatcagta
     3661 cagggttgtt tttggttcgg cgttgcaata cctgaatcgc acgacgaatc tcatcatcac
     3721 gaccgataac agggtcgagt ttgccgtcgc gggcgcgctg ggtcaggtcg agcgtgtatt
     3781 ttttcaaagc atcgcgttgg tcttcggcat tggcatcgtt cacgttttgt cctcctcgta
     3841 ccgcatcaat cgcggcatta atattttgtt cggtcgcacc ggcctctttc aaaattttgc
     3901 cggtcgcatc gttctgctga accaaagcca acaggaaaag ttcactggca atataggcat
     3961 cgccgcgctt agttgcagcc ttgtccatca agttcaatac agcttgcagc tcacggctgg
     4021 gcagaatgtc gccgccctgg ctggacactt tcggcaggct gttcaaatgc tgctgcaaac
     4081 gctgtttcac ctgcggcacg ttcacgcccg aatgcgccag caacgcggcg gctccgctgt
     4141 tttggtcgtc aagcagggct tttaacacaa agcccgcttc cagatagctg ccgtccgcag
     4201 ccaacgccaa actctgggct tctgcaaggg cttgttggaa tttggcggtt aatttgtcgt
     4261 atcgcatttt tgtttccttt tcaaaatgtc cgctgtcgaa gcctatatgt gcataattgt
     4321 ggataactca agttctgttt tctgtttttc tatatttaat tcgatatatc attgaattta
     4381 aagtatataa aaatgtataa taatgtgtat aactatatct tcttaatatg gaaaagtctg
     4441 ttgtcggctg gatgtaggtg gcaaatcggg tataatcggc gcatcttttt ccctttcaga
     4501 cggcattgat gccgcaagga catttttatg agcaaaaaac gagttctgac cggcgtaacc
     4561 accaccggca tcccgcatct gggcaactac gtcggtgcta tccgccccgc cgtccgcgcg
     4621 gcgcaaaacc ctgataccga atccttcctc ttcctcgccg attaccacgg tatcatcaaa
     4681 tgccacgagc cggagatgat tcaccaatcc acccaagccg ttgccgccac ttggcttgcc
     4741 tgcggactcg accccgagcg caccaccttc taccgccaaa gcgacattcc cgaagtgatg
     4801 gaattgaact ggattctgac ctgcatcacc gccaagggtt tgatgaaccg cgcccacgcc
     4861 tacaaagccg ccgtgcaggc aaatgcggaa aacgggcagg aagaccccga tttcggcgtg
     4921 gaaatgggtt tgttcagcta tccgattctg atgaccgccg atattctgat gttcaacgcc
     4981 aacgaagtgc ccgtcgggcg cgaccaaatc caacacgtcg aaatggcgcg cgacatcgcc
     5041 ggccgcttca atcaccgctt taaagaaatc tttaccctgc ccgaagtgaa aatcgacgaa
     5101 aacgtcgaac tcttggtcgg tttggacgga cgcaaaatgt ccaaatccta cggcaacacc
     5161 attccgcttt gggaaaacga caaaaaaacc caaaaatcgg tcaacaaaat catcaccaat
     5221 atgaaagagc cgggcgagcc gaaacagccc gacgaaagcc cattgtttga aatctacaaa
     5281 gccttctcca cgccgtctga aaccgcagcg ttcacgcaaa tgcttgccga aggcttggct
     5341 tggggcgagg caaaaaaact tttggcggcg aaaatcaacg ccgaactcgc cgaaccgcgc
     5401 gaacgctaca acgagctgac cgccgaccct tcgcaaatcg aagagatttt gcaggcaggc
     5461 gcgcaaaaag cgcgtaaaga agcacgcgaa ttgttggaca aagtacgcga tgcggtcggc
     5521 atccgcccgt tgaaataaac ccgatgccgt ccgaacctct taaaggcttg ccggaacaaa
     5581 atgtcgtccg gctgaccgga aagcatcccg gcgacttgga agccgtcggc ggcaaatgca
     5641 tggaaaccga cggaaagggc gcgccttcgg gctgggcggc aaacggcgtg tgccatacct
     5701 tgtttgccaa actggtgggc aatatcgccg aagacggcgg caaactgacg gattacctga
     5761 tttcgcattc cgccctgcaa ccctatcagg caggcaaaag cggctatgcc gccgtgcaga
     5821 acggacgcta tgtgctggaa atcgacagcg agggggcgtt ttatttccgc cgccgccatt
     5881 attgaggtat tcggacatcc cggaatatat ttgggttttt caaaccctgc aggaaaaagt
     5941 ccgcaccgtc gggaaactca aaaggagggg gatatgtgtt acaattttcc gaactgtttt
     6001 cataaaatag ttttcggacg tgtttcaata tggcattgat gccgccttat tgttcgagaa
     6061 aaaaccttta tatttaaata taatgggttt taactaaacg ggaaaccgtt ttctctccgg
     6121 ccgatgggca aaatcagccg attgatggaa cacggtccga tttttaagca aaaccttaat
     6181 caacaactca agaaggataa gacatgaaaa aattattgat tgccgcaatg atggcggctg
     6241 ccttggcagc ttgttcgcaa gaagccaaac aggaggttaa ggaagcggtt caagccgttg
     6301 agtccgatgt taaagacact gcggcttctg ccgccgagtc tgccgcttct gccgtcgaag
     6361 aagcgaaaga ccaagtcaaa gatgctgcgg ctgatgcaaa ggcaagtgcc gaggaagctg
     6421 taactgaagc caaagaagct gtaactgaag cagctaaaga tactttgaac aaagctgccg
     6481 acgcgactca ggaagcggca gacaaaatga aagatgccgc caaataattt gttgccttgg
     6541 caaaaatgat gggatgccgc ctgccggcaa cccaaacgaa accgcctgaa gattttcagg
     6601 cggtttttga gttgtatgcc ccttgttttt tacgcctgca tgaccgttcc gagcagatgt
     6661 gccgcgcata tcgggatgtt gcggacaggc aaatgccgtc tggacagggt ttggacggaa
     6721 tttgttccgt aaggttcaga cggcctcgac tttaaacggc atattgattt tttgccattg
     6781 gacgctttca tattccaatg cgtcggcaat caggggatgg cgttccagcc attccctgtc
     6841 aatacgcagg atgaagccgc agctttccgt atccgtgcgc aactgcatat ttttcgggaa
     6901 agacaggtct tggcgcgaac ggcagaacag ggcggcaagg cgcagggaca aaacggcata
     6961 ccacaacatt tcgttggtgc cgatgatgcc gctcattttt ttcatatcgc cgcgatgacc
     7021 gatgaccagt tgggcaagta tggtctgttc tttgcgtgag aaacccggca tatcggcgtt
     7081 ttcgaggatg taggcggaat gcttgtgata gccggtgtgg gcgatgtcca aaccgatttc
     7141 gtgcagcgcg gcggcgcgtc cgagatactg ttgccacaag gcaagctctt gaactgtaac
     7201 gtttttagcg tggcagaggc tgtccataaa ggtttgcgcg gtctcggcgg tgcgtttcgc
     7261 ctgattgagg ctgacgtggt agcggtgttg gaactcggca accgtttgtc cgcgcatatc
     7321 ttcgtttaaa ccgcgcccga tcaaatcgta aaacacgccg tcgcgcaggg cggcttcggt
     7381 tacggtcatc ctgtcgagtt tcatttcctc aaacgccgcc atcatcacgg caagtccgcc
     7441 ggcaaaaact tcgatgcgtt ccggtttcag gttttcaaat ttggcttttt tgaccgaacc
     7501 ggcttcgatg atgcgttcgg cgagggcgcg catgcctttg taggtaatgt ccgcctcttg
     7561 gggcatttcg gcggcaagca cgtcgcggat ggattttgcc gaacccgatg tgccgacggc
     7621 gaaatcccaa ccttcgcgcc tcatattttt gctgatgcgc tggatttcgt tgcgggcggc
     7681 ggaaatggcg gcttggaagt ctttggcggt gattttgttt tggaagaagc gcaggctgta
     7741 ggtaacgcag cccaagggca ggctttcggt aatgtcggga ttcagcgtcg agccgatgac
     7801 aaattctgtc gaaccgcccc cgatgtcgat aaccagcatt ttgccgccgc ccggggggag
     7861 ggtgtggatc acgccggtat aaatcagccg cgcctcttcg cgcccggcga tgatttcgat
     7921 ggggaaaccc agtgccgctt cggccttggg aaggaaatct gcgatgtttt tggcaacgcg
     7981 gaatgtgttg gttgccacgg cgcgcacctg ttcggggcgg aagccgcgca ggcgttcgcc
     8041 gaattttgcc agacagtcca aagcctgttc ttgggaagcg gcactcaggt ttttctgttc
     8101 gtccagtccg gcggcgaagc gcaccatctg tttgaacgaa tcgatgactt tcaactgtcc
     8161 gttgttgttt tcgcaaatct ggaggcggaa actgttggaa cccaaatcga cggaggcgag
     8221 gacgtttgcg ggatgggtgg tcatggtgga taccggtggg ggaaaaacgc aatgttactc
     8281 tgacggcgca ggcgttgaca ataaatgatg cggctgtttt tgattctgcc cacggatgtt
     8341 gccgacggca ttttttgcgc ttatttgaaa tccttttcca cgctcatgaa aatctgcgtg
     8401 tttttgcgtg tgtaaaaact tttcatattg ctgtcgattt tcagatagcg gaaattgagt
     8461 tgcggcgtaa agcccttcca agaaatcttg tcgtgccaca acgacaggtt tgcctgatat
     8521 tcgtggtctt tgcgcgggaa gcggtacaca atggtcccgg gcgcgtcaaa catcctgcgg
     8581 gtgtagcgca ggtttgcccg cagacccaag ccgccgtcga acgttttgac cgcgccgata
     8641 cgcaaaccct tgcggacgga agcctgttcc gcctccttcg tcaagtcgtg cgaccagtcc
     8701 gccccgccgt aaagcagcca gtctttcggc gcggaataca tcagcgtcgc gcccgccagc
     8761 ggcatatggc tgtcgtatcg ggcggcggtg cggtcttcct gataacgctt ccacatattg
     8821 cctgcgttca gcgtcagccg ccagtgttcg cccaagcgtt gggagaaatc ggcattgaag
     8881 ccgccgacga aattgtatcg gctgccgcct aagaggtttt gcccgacaaa cggcacgatg
     8941 ccgaacgagc gcgttaccga acgattttta tagccgagcg acaggcgcag gcactgttcg
     9001 ctgaaatctt tgttatccca ataatgcacg ccgccgccgc tgatgccgcc gtagaggaaa
     9061 tgatgcccgc ccgcattgat ttcgcgcgac acgcctaagc cctagcgcaa accgtgtgcc
     9121 ttttgcggca ggctgtcggc ggttttcgtc cagcttctgc ccgcaaattc aatcgttttt
     9181 ttggacgaag cgttgttta
//
LOCUS       DAXHSF010000070         9193 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.070, whole genome shotgun sequence.
ACCESSION   DAXHSF010000070 DAXHSF010000000
VERSION     DAXHSF010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9193
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.070"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            242..1699
                     /locus_tag="ACFM8S_001183"
     CDS             242..1699
                     /locus_tag="ACFM8S_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HFC6314259.1"
                     /translation="MSRHPAPTGEKTFFGHPFQLSTLFHIELWERFSFYGMQGILLIY
                     LYYTADKGGLGIDKTLAGGIVGAYSGSVYLSTILGAWFADRVWGAEKTLFLSGIVVML
                     GHIVLAAAPGLYGLLIGLIFIALGSGGVKSTASSMVGALYEQDEMRPLRDAGFSIFYI
                     AINIGGFLGPLLTGLLQENIGFHYGFGAAAVGMAFGLWRYSLGRKNLPHPIVPHPLSK
                     GQGKTAAAVGIALIAALATAIKTGLVNLDNFSGILLSTVILAVIAYFARLLTNPRVSS
                     DNKRHIIAYIPLFLTICMFWAVWFQIYTVATVYFDETVNRTIGSFTVPVAWKDSMQSL
                     WVILFSGLMAAMWTKMGRKQPKTPLKFAMAVFVTGASFLGFVPFISSGTPMPIAVFAL
                     IVLAITIGELMISPIALSISTKIAPPLFKTQMVALNFLAFSLGFTLGGVLFEKGYQAG
                     DEIGFYRLLFYIGAATGFLLLLLVPKLNKMLEGTD"
     gene            complement(2171..2530)
                     /gene="rnpB"
                     /locus_tag="ACFM8S_001184"
     ncRNA           complement(2171..2530)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACFM8S_001184"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(2609..3712)
                     /gene="prfB"
                     /locus_tag="ACFM8S_001185"
     CDS             complement(2609..3712)
                     /gene="prfB"
                     /locus_tag="ACFM8S_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFC6314260.1"
                     /translation="MEAEVINQLNNTLNDLEKRSEDIRVYMDYQGKKDRLEEVIGLSE
                     DPELWNDPKRAQEIGKERKILEGIVLTLDNIASGIEDNRMLIEMTVEENDEEGFAAVQ
                     EDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAER
                     KGFKIEILEEDDGEIAGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTS
                     FASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHEPTGIVVQC
                     QNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSR
                     IKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV"
     gene            complement(3815..4708)
                     /locus_tag="ACFM8S_001186"
     CDS             complement(3815..4708)
                     /locus_tag="ACFM8S_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFC6314261.1"
                     /translation="MVTFSKIRPLLAIAAAALLAACGTAGNNAARKPVQTAKPAAVVG
                     LALGGGASKGFAHVGIIKVLKENGIPVKVVTGTSAGSIVGSLFASGMSPDRLELEAEI
                     LGKTDLVDLTLSISGFIKGEKLQNYINRKVGGRQIQQFPIKFAAVATDFETGKAVAFN
                     QGNAGQAVRASAAIPNVFQPVIIGRHTYVDGGLSQPVPVSAARRQGANFVIAVDISAR
                     PGKNISQGFFSYLDQTLNVMSVSALQNELGQADVVIKPQVLDLGAVGGFDQKKRAIRL
                     GEEAARAALPEIKRKLAAYRY"
     gene            5050..5499
                     /locus_tag="ACFM8S_001187"
     CDS             5050..5499
                     /locus_tag="ACFM8S_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFC6314262.1"
                     /translation="MKIGTTWQTASAMLVLRLFAAYEFLESGLQKWNGENWFSEINDQ
                     FPFPFNLLPDALNWNLSMYAELLLPVLLLLGLATRLSALGLMVVTAVAWAAVHAGSGY
                     NVCDNGYKMALIYIVVLIPLLFQGAGGWSLDTLLKKRFCPQCRLKQD"
     gene            5573..5881
                     /locus_tag="ACFM8S_001188"
     CDS             5573..5881
                     /locus_tag="ACFM8S_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314263.1"
                     /translation="MNKNIAAALAGALSLSLAAGAVAANKPASNATGVHKSAHGSCGA
                     SKSAEGSCGAAGSKAGEGKCGEGKCGATVKKTHKHTKASKAKAKSAEGKCGEGKCGSK
                     "
     gene            5997..6839
                     /locus_tag="ACFM8S_001189"
     CDS             5997..6839
                     /locus_tag="ACFM8S_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HFC6314264.1"
                     /translation="MIQHAGLGYRRDLAEDFLSLSENSPICFIEAAPENWLKMGGWAR
                     KQFDRVAERLPLALHGLSMSLGGQAPLDTDLIDGIKEMMRRYDCTFFSDHLSYCHDGG
                     HLYDLLPLPFTEEMVHHTARRIREVQDRLGCRIAVENTSYYLHSPLAEMNEVEFLNAV
                     AREADCGIHLDVNNIYVNAVNHGLLSPEAFLKNVDAERVCYIHIAGHDVETPELLIDT
                     HGAAVLPTVWDLLELAYAKLPTIPPTLLERDFNFPPFAELEAEVAKIADYQTRAGKEY
                     RRAA"
     gene            6829..7575
                     /locus_tag="ACFM8S_001190"
     CDS             6829..7575
                     /locus_tag="ACFM8S_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HFC6314265.1"
                     /translation="MQPETSAQYQHRFAQAIRGGEAADGLPQDRLNVYIRLIRNNIHS
                     FIDRCYTETRQYFDSEEWSRLKEGFVRDAHAQTPYFQEIPGEFLQYCQSLPLLDGILA
                     LMDFEYTQLLAEVAQIPDIPDIHYSNDSKYTPSPAAFIRQYRYDVTDDLHEAETALLI
                     WRNAEDDVMYQTLDGFDMMLLEIMGSSALSFDTLAQTLVEFMPEDDNWKNILLGKWSG
                     WTEQRIIIPSLSAISENMEGNSPGQNHLSA"
     gene            7597..8184
                     /locus_tag="ACFM8S_001191"
     CDS             7597..8184
                     /locus_tag="ACFM8S_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFC6314266.1"
                     /translation="MPLPDLTDAELIESRKLLLHFARLQLPDHPDLAEDLVQETLLSA
                     YSAGDSFQGRALVNSWLFAILKNKIIDALRQIGRQRKVFTTLDDELLDEAFESHFSQN
                     GHWTPEGQPQHWNTPEKSLNNNEFQKILQSCLYNLPENTARVFTLKEILGFSSDEIQQ
                     MCGISTSNYHTIMHRARESLRQCLQIKWFNQENPK"
     gene            8190..8369
                     /locus_tag="ACFM8S_001192"
     CDS             8190..8369
                     /locus_tag="ACFM8S_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HFC6314267.1"
                     /translation="MKKCRDIALLLSKHQDRETTPGEKISIYTHLLFCPHCREYKRQL
                     QTIKISLAKTTRTSK"
     gene            8601..9041
                     /locus_tag="ACFM8S_001193"
     CDS             8601..9041
                     /locus_tag="ACFM8S_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HFC6314268.1"
                     /translation="MIKETLMRPIFLSFVLLPILITACSTPDKSARWENIGTISNGNI
                     HTYINKDSVRKNGNLMIFQDKKVVTNLKQERFANTPAYKTAIAEWEIHCNNKTYRLSS
                     LQLFDTKNTEISTQNYTASSLRPMSILSGTLTEKQYETVCGKKL"
BASE COUNT         2302 a         2498 c         2094 g         2299 t
ORIGIN      
        1 gccctatcgg ataagccctt aacagaaaag gctttacccg cgccgtatcg gaacacatcc
       61 tctaaaatac aatccgttga attgaaaaaa atataaaaac atccgcccgc gaaaaacggc
      121 agcgcgtcgt ttgacaaaga atgaaaatat cggttaaaaa ccgattttca tacaaaaaac
      181 accgctgccg tccgcatccg tttcagacgg tattgagaga aaatctttta ggagaacctt
      241 tatgtcccgg catcccgccc ccaccggaga aaaaacattc ttcggccacc ccttccagct
      301 ttccaccctc ttccatatcg aattgtggga acgtttttca ttttacggaa tgcagggcat
      361 cctgctgatt tacctctact acaccgccga caaaggcggc ttgggcatag acaaaaccct
      421 cgccggcggc attgtcggcg catacagcgg cagcgtgtac ctgtccacca ttttgggggc
      481 gtggtttgcc gaccgagtat ggggtgcgga aaaaaccctc ttcctctcgg gcatcgtcgt
      541 gatgctcgga cacatcgtcc ttgccgccgc cccgggcctg tacggccttt taatcgggct
      601 gatattcatc gcattgggca gcggcggtgt gaaatctacg gccagctcta tggtaggcgc
      661 attatacgaa caggacgaaa tgcgcccgct gcgcgatgcg ggattttcca ttttctacat
      721 cgccatcaat atcggcggct tcttaggccc gctgctgacc ggcctgctgc aggaaaacat
      781 cggtttccat tatggtttcg gcgcggcggc ggtcggtatg gcattcggct tgtggcgtta
      841 ttccttggga cgtaaaaacc tgccccatcc catcgtcccc catccgcttt caaaaggaca
      901 gggcaaaact gcggccgccg tcggcattgc tctcatcgcc gcacttgcaa ccgccatcaa
      961 aaccgggctt gtcaacctcg acaatttctc cggcatcctc ttatctaccg tcatccttgc
     1021 cgtcattgcc tattttgccc gcctgctgac taatccccgc gtcagttccg acaacaaacg
     1081 gcacatcatc gcctacatcc cgcttttcct gaccatctgt atgttttggg cggtctggtt
     1141 tcagatttac accgtggcaa ccgtctattt cgacgaaacc gtcaaccgca ccatcggttc
     1201 gtttaccgta cccgtcgctt ggaaagattc tatgcaaagc ctgtgggtca tcctgttttc
     1261 cggactgatg gcggcaatgt ggacaaaaat ggggcgcaaa cagcccaaaa ccccgctgaa
     1321 attcgctatg gcggtatttg ttaccggcgc gtcgtttttg ggattcgtcc cctttatttc
     1381 ctccggtacg ccgatgccta ttgcggtttt cgcactgatt gtccttgcca tcacgatagg
     1441 cgaactgatg atttccccga ttgcgctgtc catctccacc aaaatcgcac cgcctttatt
     1501 caaaacccaa atggtcgccc ttaatttcct tgccttttca ttaggcttca ctttgggcgg
     1561 cgtattgttt gaaaaaggct atcaggcggg cgacgaaatc ggcttctatc ggctgctgtt
     1621 ctacatcggc gcagccacag gcttcctgct gctcctgctc gtccccaaat tgaacaaaat
     1681 gctcgaaggc acagactaag tcccgccccg atgccgtctg aacccttcag acggcatttt
     1741 tccgcataat gaaaccaaac cgtttccacc cgacaggaca ggctcccccc aaccggaagg
     1801 cagcctgccg attgtcattt aaatatctga ctgattccgt ctgtttgcaa gggaaatagc
     1861 cgtttatttc cgcttgggcg gaaaccggtt ctattgagta aaaggcattt tatccgacta
     1921 aattagtgct gcatcaacgg aatatatagt ggattaacaa aaatcaggac aaggcgacga
     1981 agccgcagac agtacaaaca gtacggaacc gattcacttg gtgcttcagc accttagaga
     2041 atcgctctct ttgagctaag gcgaggcaac gccgtactgg tttttgttaa tccactataa
     2101 aaacacaacc taaataaaaa tgccgtctga accatatttc aggtttcaga cggcatttgc
     2161 gtgtcggatg cacaccggac aggcggtaag ccgggttctg tctcggacag tcattcctct
     2221 aggcataccg ttaccagtat gctcaagcaa cctacccgaa cgctcggcgg gcagcgtcat
     2281 tgcgttctgt ttggtcttgc tccgaatggg gtttggcctg ccgcatattg ttaccaaatg
     2341 cgcggtgcgc ccttaccgca ccttttcacc cttgcctgtg ctgccaaagc agccatcggc
     2401 ggttttgctt tctgttccac tttccgtcgc gttaccgcgc ccggccgtta accggcattc
     2461 taccctgcgg agcccggact ttcctccccg tatgccttac gcgatacgcg gcgactgtct
     2521 gcccgtcccg tgtgcggcgc ggattataac acgaaacgca aaaatgccgt ctgaaacggt
     2581 acaggtttca gacggcatac ggttcaaact acacgccctg tttcaggctg gcttcgataa
     2641 agccgtccaa atcgccatcc aatacggctt tggtgttgcc gacttcgtag cctgtacgca
     2701 agtctttgat acgtgaggaa tccaaaacat acgaacggat ttggctaccc caacctacat
     2761 cggatttacc ttcttccaac gcctgtttct cttcattgcg tttgcgcatt tccaattcat
     2821 acagtttgga cttcaacatt tccatcgcgg cggctttgtt ggcgtgttgc gaacggtcgt
     2881 tttgacattg caccacaatc cccgtcggct cgtgggtaat gcgcacggcg gagtcggttt
     2941 tattgatgtg ctgaccgccc gcgcccgatg cgcgataggt gtcgatacgc aaatcggcgg
     3001 ggttgatttc gatttcaatg gaatcatcga tttcagggta aacgaacacg gaggcaaacg
     3061 aagtatggcg tttgttgttc gagtcaaacg gtgaatagcg caccaagcgg tgaacgccgg
     3121 tttcggtacg cagcaaacca taggcgtatt cgccttccac tcggatagtc gcgcggttga
     3181 tgcccgcgat ttcgccgtcg tcttcttcga ggatttcgat tttgaagcct ttgcgctcgg
     3241 cgtagcggct gtacatacgg aacagcatac ccgcccagtc ttccgcttcc gtaccgcccg
     3301 cgcctgcggt gatgtcgata aagcagttgt tcgggtcggc gggctggttg aacatccgtt
     3361 tgaactccaa atccgccatc tgtttttcca gccccgccac atcttcctgc acggcggcaa
     3421 aaccttcttc gtcgttttct tcgacggtca tttcaatcag catgcggttg tcttcgatgc
     3481 ccgaagcgat gttgtcgagc gtcaacacga tgccttcgag gattttgcgc tctttgccga
     3541 tttcttgggc gcgtttcggg tcgttccaaa gttcggggtc ttcggaaaga ccgataactt
     3601 cttccaatcg gtctttctta ccctgataat ccatataaac tcggatgtct tcgctgcgct
     3661 tttccaaatc attcagggta ttgttgagct ggttgattac ttcggcttcc atgattcttt
     3721 tgttctttca aaattttagg ggcgtattgt acgggattcg ggtatttttt tctatggata
     3781 aagccttctg gaaacacgtt cagacggcat agcgtcaata acggtatgcc gccagtttgc
     3841 gtttgatttc aggcaatgcg gcacgcgctg cctcctcgcc caaccgaatg gcgcgttttt
     3901 tctgatcgaa accgccgact gcacccaaat ccaaaacctg tggtttgata actacatccg
     3961 cctgccccaa ctcattttgc aacgcagaaa cgctcattac gttcagcgtc tgatcgagat
     4021 aagagaagaa accttggctg atgtttttgc ccggacgggc ggaaatatcg acggcaatca
     4081 cgaaattcgc cccctgccgc cgggcggcac tgacgggcac gggctgcgac agaccgccgt
     4141 caacatatgt atgcctgccg atgataacgg gttggaacac attgggaatg gcggcggaag
     4201 cgcgcacagc ctgcccggca ttcccctgat tgaaagcgac ggccttgccg gtttcaaaat
     4261 cagtagcaac ggcggcaaat ttgatgggaa actgctgaat ctgcctgccg ccgacttttc
     4321 ggttgatgta attttgcagc ttttcgcctt tgataaaacc actgatggac aaggttaaat
     4381 cgaccaaatc ggttttgcct aaaatttcgg cttccaattc gaggcggtcg ggcgacatac
     4441 ccgatgcaaa aaggctgccg acaatcgaac ctgccgatgt accggtaacc accttcacag
     4501 gaataccgtt ttctttcaaa accttaataa tacctacatg ggcaaatcct ttagatgcgc
     4561 cgccaccgag tgccaaaccg accactgcgg cgggtttggc ggtttgcacc ggcttgcggg
     4621 cagcattatt tcccgccgtg ccgcaggcgg caagcaacgc ggcggcggcg attgccaaaa
     4681 gcggtctgat ttttgaaaac gttaccatat tttccattcc tttatatatc gcaccccgtc
     4741 aaaaagaggg attgcttttc ttaacaaccc cctttgacag ccaagcaaat gggggctttg
     4801 ttaagtcatc atcaaaatta atatttcttt ttttccttta cggaaattat atttgaaggc
     4861 atactatcca aggtgggaat tatctcacaa caccgccgtt atccaaatat cccgcctttt
     4921 tcccttcctt tccatcaaaa tactttcttt ttatattcat taacttgtta aatcattggc
     4981 tgccgggtgt cagtttttcc gacaaaatcc gtctaatggg gtatcaacag aaccaaaaca
     5041 ggaacactta tgaaaatcgg aacaacttgg cagacggcat ccgctatgct ggttttgcgt
     5101 ctgtttgccg catatgaatt tttggaatcg ggtttgcaaa aatggaacgg ggagaattgg
     5161 ttttctgaaa tcaacgatca attcccattc ccgttcaact tgctgccgga cgcgttaaac
     5221 tggaatctct ctatgtatgc ggagctttta ctgcccgtac tgttgctttt gggtttggca
     5281 acgcgtctgt cggcattagg gctgatggtc gttaccgccg tcgcttgggc tgcggttcac
     5341 gccggttcgg gttacaatgt ctgcgacaac ggttataaaa tggctttaat ttatatcgtg
     5401 gtattaatcc cgctgctttt ccagggtgcg ggcggatggt cgctggatac gctgctgaaa
     5461 aaacggtttt gcccccaatg ccgtctgaag caagattgat tcagtcgtgg aatctgactt
     5521 taaacattcc aaccttatct cgttaacttg atattctgaa aaggaaatag aaatgaacaa
     5581 aaacattgct gccgcactcg ccggtgcttt atccctgtct ttggccgccg gtgcagttgc
     5641 tgccaacaaa ccggcaagca acgcaacagg cgttcataaa tccgcccatg gctcttgcgg
     5701 cgcgtccaaa tctgccgaag gttcgtgcgg cgcggctggt tctaaagcag gcgaaggcaa
     5761 atgcggcgag ggcaaatgcg gtgcgaccgt aaaaaaaacc cacaaacaca ccaaagcatc
     5821 taaagccaag gccaaatctg ccgaaggcaa atgcggcgaa ggcaaatgcg gttctaaata
     5881 atcccacccc ttcaaaccaa gccgcgtttt tcagtgaaat gcggcttttt taacggcaaa
     5941 caaagatttt ttaacaagca catcattctt ttgtgccatc cgaaccgggt aaaaatatga
     6001 ttcaacacgc aggcttgggc taccgccgcg acttggcgga agactttctc tcgctttccg
     6061 aaaacagccc gatatgcttt atcgaagccg caccggaaaa ctggctgaaa atgggcggct
     6121 gggcgcgcaa acagtttgac cgtgtagcgg aacggctgcc gctggcgttg cacggattgt
     6181 ctatgtcgct gggcgggcaa gcaccgctgg atactgattt gatagacggc atcaaagaaa
     6241 tgatgcgccg ttacgattgc acgtttttct ccgaccattt gagctactgc cacgacggcg
     6301 gtcatcttta cgatttgttg ccgctgccct tcaccgagga aatggtgcat catacggcgc
     6361 ggcgtatccg cgaagtgcaa gaccgtttgg gctgccgcat cgccgtggaa aacacgtcct
     6421 actatctgca ttccccgctt gccgagatga acgaggtcga gttcctcaac gccgtcgcac
     6481 gtgaggccga ttgcggcatt catctggatg tgaacaatat ctacgtcaac gccgtcaatc
     6541 acggtctgct gtcgccggag gcttttttga aaaacgttga cgcagagcgc gtgtgctata
     6601 tccatattgc cggacatgac gtggaaacgc cggaattgtt gattgataca catggcgcgg
     6661 cagttttgcc gactgtttgg gacttgctcg aacttgccta tgccaagctg ccgacgattc
     6721 cgcccaccct gttggaacgc gattttaatt tcccgccttt tgccgaactc gaagccgaag
     6781 tcgccaaaat cgccgattat caaacgcgtg ccggaaagga ataccgccgt gcagcctgaa
     6841 acctccgccc aataccagca ccgtttcgcc caagccatac gcgggggcga agccgcagac
     6901 ggtctgccgc aagaccgact gaacgtctat atccgcctga tacgcaacaa tatccacagc
     6961 tttatcgacc gctgctatac cgaaacgcgg caatactttg acagcgaaga atggagccgt
     7021 ctgaaagaag gtttcgtccg cgacgcgcac gcccaaacgc cctattttca agaaatcccc
     7081 ggcgagttcc tacaatattg ccaaagcctg ccgcttttag acggcatttt ggcactgatg
     7141 gattttgaat atacccaatt gctggcagaa gttgctcaaa ttccggatat tcccgacatt
     7201 cattattcaa atgacagcaa atacacacct tcccctgcgg cctttatccg gcaatatcga
     7261 tatgatgtta ccgatgattt gcatgaagcg gaaacagcct tgttaatatg gcgaaacgcc
     7321 gaagatgatg tgatgtacca aacattggac ggcttcgata tgatgctgct agaaataatg
     7381 ggttcctccg cgctttcgtt tgacaccctc gcccaaaccc ttgtcgaatt tatgcctgag
     7441 gacgataatt ggaaaaatat tttgcttggg aaatggtcag gctggactga acaaaggatt
     7501 atcatcccct ccttgtccgc catatccgaa aatatggaag gcaattcccc gggccaaaac
     7561 catctatccg cataaaatta ccttgttccc gatactatgc cgctacccga cctgaccgat
     7621 gccgaattaa tagagtcgcg taaactgctt ctgcattttg cgcggcttca gttgcccgac
     7681 caccctgatt tggctgaaga tttagtgcag gaaacattgc tgtccgcata cagcgcaggc
     7741 gacagttttc aaggcagggc acttgtcaac agctggcttt ttgccatatt gaaaaacaaa
     7801 attattgacg cattacgtca aatcggaagg cagaggaaag tctttaccac actggatgac
     7861 gagctactgg atgaagcatt tgaaagccat ttttcccaaa acgggcattg gacgccggaa
     7921 gggcagccgc aacattggaa cactccggaa aaatcattaa acaacaacga attccaaaaa
     7981 attctgcaaa gctgcctata caacttgcct gaaaacaccg cacgggtatt taccctgaag
     8041 gaaatactcg gtttttcatc cgacgaaata caacaaatgt gcggtatcag cacgtccaac
     8101 taccacacca ttatgcaccg cgcccgagaa tcattgcgcc aatgcctgca aatcaaatgg
     8161 ttcaaccaag aaaacccgaa gtaaacgtta tgaaaaaatg ccgcgatatc gccctgcttc
     8221 tttccaaaca tcaggaccgg gaaaccaccc cgggcgagaa gatttccata tacacacacc
     8281 tgctgttctg cccacattgc cgagaatata aaagacagct tcaaaccatc aaaatatcac
     8341 tggcaaaaac aaccagaact tcaaaataaa tgccgtctga aaaggcttca gacggcataa
     8401 gctgacggaa aaaaatcaaa ccgatttact gttatcggca gttcatccat aatacacgct
     8461 tcaaaagcag catattcctc catacggaat atataaatac gcaaaacacg aaggctgcat
     8521 caatttgctt tatttgcctt atttcacaga cggcgctacc cctcccgccc aacccgttct
     8581 ttctgaatga gcagatttca atgattaagg aaaccctaat gcgcccaatc ttcctatctt
     8641 tcgttttact ccctattttg ataaccgcct gcagcacacc ggacaagtct gcccgatggg
     8701 aaaatatcgg cacaatctca aacggcaata ttcatacata tatcaataaa gacagcgtga
     8761 gaaaaaacgg aaatctgatg attttccaag ataaaaaagt tgttaccaat ctaaaacaag
     8821 aacgttttgc caacaccccc gcatacaaga ctgccattgc cgagtgggaa atccactgca
     8881 acaacaaaac ataccgctta agttcgctac agttgtttga tacaaaaaac acggaaattt
     8941 ccacacaaaa ctacacagcc tcttccctcc gcccgatgag catcctgtcc gggacattaa
     9001 ccgaaaaaca atatgaaacc gtatgcggaa aaaaactctg attgcaactt atacacaaac
     9061 ctacccacaa accttgtcat aaaaatgccg tctgaaatac tgaaatatca gcatttcaga
     9121 cggcattttg ccattccctg aaaattatcc acaaagttat ccacattatt tttaaaaccg
     9181 gcttccatcc gaa
//
LOCUS       DAXHSF010000071         9126 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.071, whole genome shotgun sequence.
ACCESSION   DAXHSF010000071 DAXHSF010000000
VERSION     DAXHSF010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9126
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.071"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(59..1315)
                     /locus_tag="ACFM8S_001194"
     CDS             complement(59..1315)
                     /locus_tag="ACFM8S_001194"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HFC6314269.1"
                     /translation="MNAAQLDHTAKVLAEMLTFKQPADAVLSAYFREHKKLGRQDRHE
                     IAETAFAALRHYQKISTALRRPHAQPRKAALAALVLGRSTNISQIKDLFDEEETAFLG
                     NLKARKTEFSDGLNTAAELPQWLVEQLQQHWREEEILAFGRSINQPAPLDIRVNTLKG
                     KRDKVLPLLQAESADAEATPYSPWGIRLKNKIALNKHELFLDGTLEVQDEGSQLLALL
                     VGAKRGEIIVDFCAGAGGKTLAVGAQMANKGRIYAFDIAEKRLANLKPRMTRAGLTNI
                     HPERISSEHDSRIARLAGKADRVLVDAPCSGLGTLRRNPDLKYRQSAETVANLLEQQH
                     SILDAASKLVKPQGRLVYATCSILPEENELQVERFLSEHPEFEPVNCAELLAGLKIDL
                     DTGKYLRLNSARHQTDGFFAAVLQRK"
     gene            complement(1663..2004)
                     /locus_tag="ACFM8S_001195"
     CDS             complement(1663..2004)
                     /locus_tag="ACFM8S_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314270.1"
                     /translation="MKNNVKNWTTKEVKQSLDKFNNILIKNTFLLQYLKKEFSASSAY
                     CLSMLPEEEDIYEILVNGNIIIDLEFNKHTNETVVINVTDVDEYLKTLTNESGRVFFT
                     LAKEIGKQKNI"
     gene            complement(2208..3065)
                     /locus_tag="ACFM8S_001196"
     CDS             complement(2208..3065)
                     /locus_tag="ACFM8S_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HFC6314271.1"
                     /translation="MNYALDALWWKLTSQPVRDLASLLTAPPLWQSGCELGVRELLGE
                     RGFRYLLALDADPAPLTEHLTRRAPFGHRLGIYAEELLAFWFANAPHAELLAHNLTVS
                     GSDGNTQGAADFVVRLNGKPYHIELTCKYYGGGTGRLEDMRGLDPKDTLLGKAAKLTA
                     QLGLPHTSDGIRTLRQHGLPLDVKPVSIVRGIGFFPHGFHAFEPPLNPYGWRGIYIQD
                     WAEYGFKRQEVRYHLLDRMAYLAPARVAETETLNATEIRRIDQGLIAVLECRPDGFWH
                     EIERIMKAV"
     gene            complement(3126..3941)
                     /locus_tag="ACFM8S_001197"
     CDS             complement(3126..3941)
                     /locus_tag="ACFM8S_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HFC6314272.1"
                     /translation="MTTLKPALPAYLDNIRIILTRTSHPANIGSAARAMKTMGLHKLT
                     IVAPNLMATPMTENPPVFDPEHPQSFKLPEESFILASGAADVLENATIAASLDEALAD
                     TTIACALTSRRREITAPLQTPRDLVSELLQTANRGEKVALVFGNETFGLSIEEVQACN
                     RLMTINGNPDYFSLNLAQAVQVVCYEIFSQTGSPMTHLQQEDHAATHEQIKGMVAHME
                     SVMNDIGFFNRRNGERLIRRMQSLFGRANTQTEDIDILRGFFNTVSHRIHKKD"
     gene            4005..4175
                     /locus_tag="ACFM8S_001198"
     CDS             4005..4175
                     /locus_tag="ACFM8S_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314273.1"
                     /translation="MPSEMFFNNVGNLTLRLKQTRVGRYFTRFGRIYPNQINSDFAAP
                     KDCASEPAVCHP"
     gene            4185..4970
                     /locus_tag="ACFM8S_001199"
     CDS             4185..4970
                     /locus_tag="ACFM8S_001199"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFC6314274.1"
                     /translation="MNPFLNTAFKAARRAGQMMIRAAGNLDAVKVDSKAFNDFVSDVD
                     RNSEIILVEALKEAYPHHKITCEEGGFHGKAAAEYEWIIDPLDGTTNFLHGHPQYAIS
                     MALLHKGVLQEALVYAPERNDVYMASRGKGALLNDRRIRVSNRIELNRCLIGTGFPVV
                     DQSMMDKYLAILKDFLAKTAGGRREGAASLDLCAVATGRFDGFFEFNLKPWDIAAGAL
                     IVQEAGGIVTDMTGEDAWLESGDIVAANPKVLAQMLKIISAHV"
     gene            5239..7358
                     /locus_tag="ACFM8S_001200"
                     /pseudo
     CDS             5239..7358
                     /locus_tag="ACFM8S_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219143.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            7482..9032
                     /locus_tag="ACFM8S_001201"
     CDS             7482..9032
                     /locus_tag="ACFM8S_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFC6314275.1"
                     /translation="MKKPLISVAAALLGVALGTPYYLGVKAEESLTQQQKILQEAGFL
                     TVESHQYERGWFTSTETTVIRLKPELLNNARKYLPDNLKTVLEQPVTLVNHITHGPFA
                     GGFGTQAYIETEFKYAPETEKVLERFFGKQVPVSLANTVYFNGSGKMEVSVPAFDYEE
                     LSGIRLHWEGLTGKTVYQKGFKSYRNGYDAPLFKIKLADKGDAAFEKVHFDSETSDGI
                     NPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEV
                     GKLAFSTKTGESGAFIDSEGQFRFDTLVYGDEKYGPLDIHIAAEHLDASALTVLKRKF
                     AQISAKKMTEEQIRNDLIAAVKGEASGLFTNNPVLDIKTFRFTLPSGKIDVGGKIMFK
                     DLKKEDLNQLGLMLKKTEADIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEGRASLDD
                     INETLRLMVDSTVQSMAREKYLTLNGDQIDTAISLKNNQLKLNGKTLQNEPEPDFDEG
                     GMVSEPQQ"
BASE COUNT         2284 a         2168 c         2422 g         2252 t
ORIGIN      
        1 atgaaatgcc gtctgaacgc tttgaaaagg gttcagacgg cattttgttt aaacagggtt
       61 atttgcgttg caatacggcg gcgaagaagc cgtcggtttg gtgtcgggcg gagttgaggc
      121 gcaggtattt gccggtatcc aaatcgattt tcaaaccggc aagcagttcg gcgcagttga
      181 cgggttcaaa ttcgggatgt tcggacagga aacgttcgac ttgcagctcg ttttcttcgg
      241 gcaggatgct gcaagtggcg taaaccaagc ggccttgcgg ttttaccagt ttggaggcgg
      301 catcgaggat gctgtgttgc tgttccaaaa ggttggccac ggtttcggcg gattggcggt
      361 atttgaggtc gggattgcgg cgtaaagtac ccaaaccgga gcagggcgcg tccaccaaca
      421 cacggtcggc tttgcctgcc agtcgggcga tacggctgtc gtgttcgctg ctgatgcgtt
      481 cggggtggat attggtcagt ccggcgcggg tcatacgcgg tttgaggttg gcaaggcgtt
      541 tttcggcgat gtcgaaggcg tagattctgc ctttattggc catttgcgca ccgacagcca
      601 aggttttgcc gccggcaccg gcacagaaat cgacaatgat ttcgccgcgt tttgcgccca
      661 ccaataaagc aagcagctgg ctgccttcgt cttggacttc cagtgtgccg tctaaaaaca
      721 gttcgtgttt gttaagcgcg attttgtttt tcaggcggat gccccaaggc gaataaggcg
      781 ttgcctctgc atcggcactt tcggcttgca acagcggcag cactttatcg cgtttgcctt
      841 tcaaagtgtt gacgcggatg tcgagcgggg caggctggtt gatgctgcgg ccgaaggcga
      901 ggatttcttc ttcacgccaa tgctgttgca gttgttcgac cagccattgc ggcaattctg
      961 cggcggtatt caggccgtct gaaaactcgg ttttacgggc tttcaaattg ccgaggaacg
     1021 ctgtttcttc ttcatcaaac aggtctttga tttggctgat gttggtgctt ctgccgagaa
     1081 ccagtgcggc gagtgcggct ttgcgcggct gtgcgtgcgg acggcgcagg gcagtgctga
     1141 ttttttgata gtggcgcagc gcggcaaagg cggtttcggc gatttcgtgg cggtcttggc
     1201 ggccgagctt tttatgttcg cggaaatagg cggagaggac ggcatcggca ggctgtttga
     1261 aagtcagcat ttcagccaaa actttggcgg tatggtcgag ttgtgcggcg ttcataatgt
     1321 aatgggttct cttactgtgg gttattcata atcaatctaa cgtccttaaa ataacaatag
     1381 catctttatc attacttagt attctatccg caataagaaa gatgtgttaa aagtgcgcaa
     1441 tcgggatttg tctaaatttc tccgttaact acaaaggcag cctgaaaaaa gttctatttt
     1501 caggtcgctt gaacagcatc cgatataatc acatcagcga tttcattttg taatcactcc
     1561 ccaaaacatg aggaaaaaaa caaaatttat tttcttcgtt cagtatatct atcctaaata
     1621 tgccatcact ccattgagtt tttaatttgt atttcgctct tgttaaatgt ttttctgttt
     1681 gccgatttct tttgctaatg taaaaaatac tctaccactc tcattggtta aagttttcaa
     1741 gtattcatca acatcagtaa cattaataac aactgtttca tttgtatgtt tattaaattc
     1801 taaatcaata ataatattcc cattaaccaa tatttcatat atatcttctt cttcagggag
     1861 catagacaaa caataagcac ttgaagctga aaactctttt ttcagatact gaagaagaaa
     1921 agtattttta attaaaatat tattaaattt atctaatgat tgcttgactt ctttagttgt
     1981 ccaattttta acattattct tcattgtatt ctacctgggg tcgtagttat taaattacca
     2041 tacttatttg tttggatcgt cataatatta gttggcatgt tgttgttata tttatcatat
     2101 ccaatatcct tattaactcg gatgactctt tcataaattg tatgggaaat gagatttcca
     2161 tcactaagcc tctcaataga tatacgcgtt aatgttggga aagggtttca gacggccttc
     2221 ataatgcgtt cgatttcgtg ccaaaagccg tccggccgac attccaaaac ggcaatcaag
     2281 ccttggtcga tacggcggat ttcggttgcg ttcaatgttt cggtttcggc gacacgcgca
     2341 ggcgcgaggt aggccatacg gtcgagcaga tggtagcgga cttcttggcg tttaaacccg
     2401 tattccgccc aatcttgaat atagatgccg cgccaaccgt atggattaag cggtggctca
     2461 aaagcatgga aaccgtgtgg aaaaaatccg atgccgcgca cgatggaaac gggttttacg
     2521 tcaagcggca gaccgtgttg ccgcaaggtt cggatgccgt ctgaagtgtg cggcagaccg
     2581 agttgggcgg tcagtttggc ggcttttccc aacagcgtgt ctttggggtc gagtccgcgc
     2641 atgtcttcga gcctgcccgt gccgccgccg taatatttgc aggtcagctc gatatggtag
     2701 ggcttgccgt taagccttac cacaaaatcc gccgcgcctt gcgtattgcc gtccgaaccg
     2761 gaaaccgtga ggttgtgcgc gagcagttcg gcgtgtggcg cgttggcaaa ccaaaatgcc
     2821 agcagttctt cggcgtaaat gccgaggcgg tgtccgaacg gggcgcgtcg ggtgaggtgt
     2881 tccgtcagcg gcgcgggatc ggcatccaat gccaaaagat agcggaaacc gcgttccccc
     2941 aatagttcgc gcacgcccaa ttcgcaaccg ctttgccaca aaggcggcgc agtcagcagc
     3001 gaggcgaggt cgcggacggg ttggctggtg agcttccacc ataatgcgtc tagggcgtaa
     3061 ttcatgctta tccgaatatc agtcatgccg tctgaaaagc tggaaatgtt ttcagacggc
     3121 cttaatcagt cttttttatg gatacgatgg ctgacggtat tgaaaaaacc gcgcaggata
     3181 tcgatgtctt cggtttgcgt gttggcgcgt ccgaacaggc tctgcatacg gcgtatcaga
     3241 cgctcgccgt tgcggcggtt gaaaaagccg atgtcgttca tcacgctttc catgtgggcg
     3301 accatgcctt tgatttgctc gtgggtcgca gcgtggtctt cttgttgaag atgggtcatg
     3361 ggcgaaccgg tttggctgaa gatttcgtag cacacgacct gcacggcttg ggcgaggttg
     3421 agcgagaaat agtcgggatt gccgttgatg gtcatcagtc ggttgcaggc ttggacttct
     3481 tcgatgctca agccgaaagt ctcgttgccg aaaaccagtg ccactttctc gcctcggttt
     3541 gcggtctgca gtaattcgga taccaaatcg cgcggggttt gcagcggcgc agtaatttcg
     3601 cggcggcggc tggtcagggc gcaggcgatg gtggtgtcgg caagggcttc gtccaaagaa
     3661 gcggcaatgg tggcattttc caaaacgtct gccgcgccgg aagcgaggat gaagctttct
     3721 tccggtaatt taaacgattg aggatgctcc gggtcaaaca cgggcgggtt ttccgtcatc
     3781 ggcgttgcca tcagatttgg ggcgacgatg gtcagtttgt gcagacccat tgttttcatc
     3841 gcgcgcgcgg cagagccgat gttggcggga tggctggtgc gcgtgaggat gatgcggatg
     3901 ttgtccagat aagcgggcag ggcgggtttg agagtagtca tgttcggttt tcggtcggaa
     3961 tggaataggg tataatgcgc cgtctttttc gttcggacgg tcaaatgccg tctgaaatgt
     4021 tctttaacaa cgtcggaaat ttaacgttgc gcctgaagca gacacgcgtc gggcggtatt
     4081 ttacccgatt tggcaggatt taccccaacc aaataaactc cgattttgcc gctccgaagg
     4141 actgcgcgtc cgaaccggcg gtatgccacc cttaaggaaa tctgatgaat ccgtttttga
     4201 atacagcctt taaagccgcc cgccgtgccg gtcagatgat gatacgcgcc gcgggcaatc
     4261 tcgatgccgt caaagtagac agcaaagcct tcaacgattt tgtttccgat gttgaccgca
     4321 attccgaaat catcctggtt gaggctttga aagaagccta tccgcaccac aaaatcactt
     4381 gcgaagaagg cggcttccac ggcaaagccg cagccgagta cgaatggatt atcgatccgc
     4441 ttgacggcac gaccaatttc cttcacggac atccccaata cgccatctct atggcactcc
     4501 tgcacaaagg cgtgttgcag gaagctttgg tgtacgcccc cgaacgcaac gacgtatata
     4561 tggcttcgcg cggcaaaggc gcgttgctca acgaccgccg catccgcgtt tctaaccgca
     4621 tcgaattgaa ccgctgcctg atcggtaccg gcttccccgt tgtcgatcaa agcatgatgg
     4681 acaagtatct ggcgattttg aaagactttt tggcaaaaac cgccggcggc cgtcgtgaag
     4741 gcgcggcttc tttggatttg tgtgctgtgg cgactggtcg ttttgacggc tttttcgagt
     4801 ttaacctcaa accgtgggac attgccgcag gtgcattgat tgttcaggaa gcgggcggta
     4861 tcgtaaccga tatgacgggt gaggacgctt ggttggaaag cggcgatatt gtcgccgcca
     4921 atcccaaagt attggcgcag atgctgaaaa tcatttccgc acacgtttaa atttcagccc
     4981 tttcccgaaa atatgggaaa acgggcgggt atgccgtctg aagtatcgcg ccgatatttc
     5041 agacggcatt tttattggca acgcggcagg acggcggaaa cggtcaaatg ccccaaactg
     5101 ccgaggatgg aaacccatat ctgattaaac ggagaaccct gtgcgatttg tttatagtgg
     5161 attaacaaaa accagtacgg tgttgcctcg ccttagctca aagagaacga ttctctaagg
     5221 tgctgaagca ccaagtgaat gggcaaagcc aacgccttta ccgcaccgat taccgtcgtc
     5281 aattacgacg aacaagccct caataacacc gaagcgcgta ctttggtgga tgccgtagcg
     5341 aaaaaagacg cttccgtttg gcagttcggc ggcgaaagca acacgctgac cggactgtat
     5401 ttcagaggtt atcagcttga tgcgcgccaa ttcagcgtca acggtttggc aggtatgtac
     5461 ggcacgcaag gcacggcaag cgtgcaagtt ggatcggcac aactgattaa aggcgcatcc
     5521 actgccgtaa acggcatgga ccctgaaggt gcggtatccg gttccgtcaa tatcgaaacc
     5581 aaaaaagccg ccgatgaagg caaccgcaaa atcggtttgg gctggttcag taacaaccgc
     5641 gcccaaggta cgttcgactt gggccaacgt ttcggcgaaa acaaagcatt cggcgtgcgc
     5701 gccaacggca aactgcgcca cggcgacacc ccgcgccacg gttacagcga agacaacaaa
     5761 gaatttgcgg taaatgccga ctatcgcggc gaaacactgc gcgtagcgtt cgattccatc
     5821 tacgcaaaac gcaaaaccaa cggcggtcgc gcgcgtatgc aggacattca aaacgccggc
     5881 ggacgcttgt ttggcgcgcc cgatggtaaa accaacctgc tgccttcttg gaactggcaa
     5941 aataccgccg gccaaaccaa tatgcttacg tttgaatggg atgcgtttga taatgcacag
     6001 attacaggcg gtatcggcta caacaaggca cgctattacg gcacgctgat ttccccgacc
     6061 gtatgtttga atgctaccag cacttgtacc gatatgcaaa acacaaacgc cagcggcaaa
     6121 gtaacggcac gcaactatga ctacaatacc ggtactgcgc gcctgaccga ccaatatttc
     6181 cgcacattga gtatgaacct gaccgcacgc ggcgaatttg aaaccggccc ggtaacacat
     6241 aactggagta tggctttcga ccgcgttatc cgccagcgga aaaccataaa cggcagcggg
     6301 aacggcaaca gcaagataga agtgaaggca aacgaaaata ttgcgaatca attggcatcg
     6361 tttacagccg actatcccaa ttcttgggag aactctgcca acttggatgc caatatcaaa
     6421 gtcaacagcc tggctttgtc cgacaccttg ggcttcgtgg ataacaaata ccgcctgact
     6481 ttgggcggac gtttccaaac agtcgaatat accgacaaaa aatcacagag cggcgatgcc
     6541 aaacgtttca gcccgatgct gatggcggcg tgggtgccgc aacctgattt ggttgtgtac
     6601 ggcaactata tggaagactt ggagcctgcc gacatcaaaa ccgatgattc cggcgaaacg
     6661 acaatggcga aaccgcgtgt cagccgccag tttgaagtgg gcgtgcgtaa aaactggggc
     6721 aatttcgtaa ccacgttaaa cgcgttccaa atcaaacgcc cggctactgg cgcggtcata
     6781 caactaccaa gacaacaaaa ggggtaacaa caacgctgac ctatggcaac aattccgatt
     6841 tcgcccgcta taaggcgcaa ggcggcgtag ccggcgacga gcaaggtatg gaacgcaatc
     6901 gcggtatcga gttcaatgcc tatgctaacc tgttaaacaa aaccctgcgt ccgactttgg
     6961 gcctgatgta tctgcaatcg accgtgaaag agtatccaaa ttcacgcgat atgctggtta
     7021 acggcgtaca agtcgccaat ccgcgcgtaa ttgccaaagc aggtttggaa tgggatacac
     7081 cgtttgccaa aggattgact ttaaacggta atgtttcgta tttcggcaag tcttaccaag
     7141 acacgcaaaa acaatacgcc ttcccgtcct ataccttgat tgacgtaggc gcgcgctaca
     7201 aaaccaagct cggcaaaaat actttgaccg tcagcagctc ggtggaaaac ctgttcaaca
     7261 aaaactattg gcaggtacag cgcggccaat acgaccgcag cttcgccgtc gtcggtatgc
     7321 cgcgcactta ctggctgaag gcggaattgg atttctgatt tccctatatc cggaaaatgc
     7381 cgtccgaaca ggtttcagac ggcatttctt ttaaagagat atggcgggat acgcttaaat
     7441 aatgctattc tagcgtcttt gaaacctgct tgtttgaagt gatgaaaaaa cctttgattt
     7501 cggttgcggc agcattgctc ggcgttgctt tgggcacgcc ttattatttg ggtgtcaaag
     7561 ccgaagaaag cttgacgcag cagcaaaaaa tattgcagga agcgggcttc ttgaccgtcg
     7621 aatcgcacca atatgagcgc ggctggttta cctctacgga aacgacggtc atccgcttga
     7681 aaccggagtt gctgaataat gcccggaaat acctgccgga caacctgaaa acagtgttgg
     7741 aacagccggt tacgctggta aaccatatca cgcacggccc tttcgccggc ggattcggca
     7801 cgcaggcgta cattgaaacc gagttcaaat acgcgcctga aacggaaaaa gttctggaac
     7861 gcttttttgg aaaacaagtc ccggtttccc ttgccaatac cgtttatttt aacggcagcg
     7921 gtaaaatgga agtcagtgtt cccgccttcg attatgaaga gctgtcgggc atcaggctgc
     7981 actgggaagg attgacggga aaaacggttt atcaaaaagg tttcaaaagc taccggaacg
     8041 gctatgatgc ccccttgttt aaaatcaagc tggcagacaa aggtgatgcc gcgtttgaaa
     8101 aagtgcattt cgattcggaa acttcagacg gcatcaaccc gcttgctttg ggcagcagca
     8161 atctgacctt ggaaaaattt tccttagaat ggaaagaggg tgtcgattac aacgtcaaat
     8221 tgaacgaact ggtcaatctt gttaccgatt tgcagattgg cgcgtttatc aatcccaacg
     8281 gcagcatcgc accttccaaa atcgaagtcg gcaaactggc tttttcaacc aagaccgggg
     8341 aatcaggcgc gtttatcgac agcgaagggc agttccgttt tgacacgctg gtttacggcg
     8401 atgaaaaata cggcccgctg gacatccata tcgccgccga acatctcgat gcttctgcct
     8461 taaccgtatt gaaacgtaag tttgcacaaa tttccgccaa aaaaatgacc gaggaacaaa
     8521 tccgcaatga tttgattgcc gccgtcaaag gcgaggcttc cggactgttc accaacaatc
     8581 ccgtattgga cattaaaact tttcgtttca cgctgccgtc aggaaaaatc gatgtgggcg
     8641 gaaaaatcat gtttaaagat ttgaagaagg aagatttgaa ccaattgggt ttgatgctga
     8701 agaaaaccga agccgacatc agaatgagta ttcctcaaaa aatgttggaa gacttggcgg
     8761 tcagtcaggc aggcaatatt ttcagcgtca atgccgaaga tgaggcggaa ggcagggcaa
     8821 gtcttgacga catcaacgag accttgcgcc tgatggtgga cagtacggtt cagagtatgg
     8881 caagggaaaa atatctgact ttgaacggcg accagattga tactgccatt tctctgaaaa
     8941 acaatcagtt gaaattgaac ggtaaaacgt tgcaaaacga accggagccg gattttgatg
     9001 aaggcggtat ggtttcagag ccgcagcagt aatgggtcaa atgccgtctg aaatgttcag
     9061 gcggcatttt gttgtattgg aaactgccgt tttcaaccat tcctttagat aagccgtgtc
     9121 ggattc
//
LOCUS       DAXHSF010000072         9070 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.072, whole genome shotgun sequence.
ACCESSION   DAXHSF010000072 DAXHSF010000000
VERSION     DAXHSF010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9070
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.072"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(129..464)
                     /locus_tag="ACFM8S_001202"
     CDS             complement(129..464)
                     /locus_tag="ACFM8S_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFC6314276.1"
                     /translation="MFKDWKEHTALVKKSFGELGKAHPKMLQAYGALEQAAAAEALDA
                     KTRELIAIAVAITTRCESCISVHAAAAAKAGATDSEIAGALATAIALNAGAAYTYALR
                     ALEAVETQK"
     gene            593..1498
                     /gene="mtrA"
                     /locus_tag="ACFM8S_001203"
     CDS             593..1498
                     /gene="mtrA"
                     /locus_tag="ACFM8S_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter MtrCDE transcriptional
                     activator MtrA"
                     /protein_id="HFC6314277.1"
                     /translation="MDILDKLVDFAQLTGSVDVQCLLGGQWSVRHETLQREGLVHIVT
                     SGSGYLCIDGETSPRPVSTGDIVFFPRGLGHVLSHDGKCGESLQPDMRQHGAFTVKQC
                     GNGQDMSLFCARFRYDTHADLMNGLPETVFLNIAHPSLQYVVSMLQLESKKPLTGTVS
                     MVNALSSVLLVLILRAYLEQDKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNVDKM
                     VAAANMSRAQLMRRFKSRVGLSPHAFVNHIRLQKGALLLKKNPDSVLSVALSVGFQSE
                     THFGKAFKRQYHVSPGQYRKEGGQK"
     gene            complement(1560..2048)
                     /locus_tag="ACFM8S_001204"
     CDS             complement(1560..2048)
                     /locus_tag="ACFM8S_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFC6314278.1"
                     /translation="MKKYLIPLSIVAVLSGCQSIYVPTLTEIPVNPINTVKTEAPAKG
                     FRLASSHWTDVAKISDEATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDSMYE
                     IYLRSAIDSQRGAINTEQSKLYIQNALRGWQQRWKNMDVKPNNPAFTNFLMEVMKMQP
                     LK"
     gene            2195..3097
                     /locus_tag="ACFM8S_001205"
     CDS             2195..3097
                     /locus_tag="ACFM8S_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFC6314279.1"
                     /translation="MDTAKKDILGSGWMLVAAACFTIMNVLIKEASAKFALGSGELVF
                     WRMLFSTVALGAAAVLRRDTFRTPHWKNHLNRSMVGTGAMLLLFYAVTHLPLATGVTL
                     SYTSSIFLAVFSFLILKERISVYTQAVLLLGFAGVVLLLNPSFRSGQETAALAGLAGG
                     AMSGWAYLKVRELSLAGEPGWRVVFYLSVTGVAMSSVWATLTGWHTLSFPSAVYLSCI
                     GVSALIAQLSMTRAYKVGDKFTVASLSYMTVVFSALSAAFFLGEELFWQEILGMCIII
                     LSGILSSIRPTAFKQRLQSLFRQR"
     gene            complement(3138..4268)
                     /locus_tag="ACFM8S_001206"
     CDS             complement(3138..4268)
                     /locus_tag="ACFM8S_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6314280.1"
                     /translation="MAKHLPLAVLTALLLAACGGSDKPPAEKPAPAENRNVLKIYNWS
                     EYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKAG
                     AYQKIDKSLIPNYKHLNPEMMRLMDGVDPGHEYAVPFYWGTNTFAINTERVKKALGTD
                     KLPDNQWDLVFDPEYTSKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATA
                     LLKKNRPNIKRFTSSGFIDDLARGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPK
                     EGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTYAPSSKPARELMEDE
                     FKNDNTIFPTEEDLKNSFIMVPIQPAALKFMVRQWQDVKAGK"
     gene            4472..7096
                     /gene="alaS"
                     /locus_tag="ACFM8S_001207"
     CDS             4472..7096
                     /gene="alaS"
                     /locus_tag="ACFM8S_001207"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFC6314281.1"
                     /translation="MKTSELRQKFLKFFETKGHTVVRSSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYSRATTAQKCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYFK
                     RDAIHFAWEFLTSPEWLNIPKDKLLATVYAEDDEAYNIWLNEIGMPSERIVRIGDNKG
                     AKYVSDNFWQMGDTGPCGPCSEIFYDHGEEIWGGIPGSPEEDGDRWIEIWNCVFMQFN
                     RDEQGNMNPLPKPSVDTGMGLERMAAVMQHVHSNYEIDLFQDLLKAVARETGAAFSMD
                     EPSLKVIADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRAVRHGYKLGQSKPFFHKLV
                     ADLVKEMGDAYPELKEKQVQIEEALKNEESRFAQTLETGMALLENALAKGGKTLDGEI
                     IFKLYDTYGFPYDLTADICRERNIELDEAGFEREMEAQRARARAAQSFKANAQLPYDG
                     QDTEFKGYSERQTESKVLALYKDGEQVNELNEGDSGAVVIDFTPFYAESGGQVGDVGY
                     IFSGENRFEVRDTQKIKAAVFGQFGVQTSGRLKVGDSVTAKVDDEIRNANMRNHSATH
                     LMHKALRDVLGRHVEQKGSLVTAESTRFDISHPQAVTAEEIAEVERRVNEAVLANVAV
                     NAAIMSMEDAQKTGAMMLFGEKYGDEVRVLQMGGFSTELCGGTHVSRTGDIGLFKIIS
                     EGGIAAGVRRIEAITGLNALKWAQEQERLVKDIIAETKAQTEKDVLAKIQAGAAHAKA
                     LEKELARAKAELAVHAGAKLLDDAKDLGAAKLVAAQIEADAAALREIVTDLTGKSDNA
                     VILLAAVNDGKVSLCAGVSKPLTGKVKAGDLVKFAAEQVGGKGGGRPDLAQAGGTDAG
                     KLPEMLVSVESWLCQKLS"
     gene            7158..7889
                     /locus_tag="ACFM8S_001208"
     CDS             7158..7889
                     /locus_tag="ACFM8S_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314282.1"
                     /translation="MNTKTELQKLLEEDISTLTETLICADALPPRYVRSIATPIVRRW
                     LIDKQLNILAKEIGLTIELPILDTSLVFEKLSTLENKVNFYMAGGVYLGGEFISSIYH
                     SSQEFSGEPIIYAEPNIILCPAEKFLTLKRVFHNGNIFNMNQIITFLSNKQGGVHFDK
                     NYDKYKTWQVAIEKAANFLKLGNPYNEDKLSLSEEHDTILVVLPLEKGYEWNCLEIEV
                     LSAAQSLANIYCNKVRLIDGHVWKE"
     gene            8316..>9070
                     /locus_tag="ACFM8S_001209"
     CDS             8316..>9070
                     /locus_tag="ACFM8S_001209"
                     /inference="COORDINATES: protein motif:HMM:NF042870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IIL restriction-modification enzyme MmeI"
                     /protein_id="HFC6314283.1"
                     /translation="MDRKNVAHFEYPVKDPADNTQFVDIFWEGIFLAEHKSANKNLTK
                     AKEQAERYLQEIGRTKPSALPEYYAVSDFAHFHLYRRVPEEGVENQWQFPLEALPEYI
                     TRGVFDFMFGIEAKVRQIQEEADIQAAAAIGRLHDALKEEGIYEEHELRLFITRLLFL
                     FFADDSAVFQRNYLFQDFLESCKETDTLGDKLNQLFEFLNTPDQKRSKTQSEKFKGFE
                     YVNGGLFKERLRTFDFTAKQHRALIDCGNFDWR"
BASE COUNT         2311 a         2380 c         2186 g         2193 t
ORIGIN      
        1 cttacccctt gtttctcaaa tgccgtctga aaaaacggct gcatatgttg tctacaaaat
       61 atcgggaagc ataacaggca aaatgccgtc tgaaatattt tcagacggca tttcttatcc
      121 gaaacggatt atttttgcgt ttcaaccgct tccaatgcac gcagggcata agtgtaagcg
      181 gcacccgcat tcagggcaat ggcggttgcc aatgcacctg cgatttcgct gtcggtcgca
      241 ccggctttgg cggcagcggc ggcgtgaaca ctgatgcagc tctcgcaacg tgtggtaatg
      301 gcaacggcga tggcaatcag ttcgcgcgtt ttggcatcaa gtgcttcggc ggcggcggcc
      361 tgttccaatg cgccgtaggc ctgcagcatt ttaggatgcg ccttacccag ctcgccgaac
      421 gattttttaa ccaatgcggt atgttctttc caatctttaa acattttctt ttcctttctc
      481 ttgcgtttaa ccctgatacg cgcttgcgta tctgttttcg atgtgcgtat tattgcaatt
      541 attcagttgt gtttctcgtt taatcatctc attttatggt tcaaaaagat ttatggacat
      601 tctggacaaa ctggtcgatt tcgcccaatt gacgggcagt gtggatgtgc agtgcctttt
      661 gggcggacaa tggtcggtac ggcatgaaac cttgcaacgc gaaggattgg tacacattgt
      721 tacatcgggc agcggctatc tctgcatcga cggcgaaact tccccgcgtc cggtcagtac
      781 aggggatatt gtatttttcc cgcgcggctt gggtcatgtg ttgagccacg acggaaaatg
      841 cggagaaagt ttacaaccgg atatgcggca gcacggtgcg tttacggtca agcagtgcgg
      901 caacggacag gatatgagcc tgttttgcgc ccgtttccgc tacgacaccc acgccgattt
      961 gatgaacggg ctgcctgaaa ccgtttttct gaacattgcc catccgagtt tacagtatgt
     1021 ggtttcaatg ctgcaactgg aaagcaaaaa acctttgacg gggacggttt ccatggtcaa
     1081 cgcattgtcg tccgtcctgc tggtgcttat cctgcgcgcc tatctcgaac aggataagga
     1141 tgtcgaactc tcgggcgtat tgaaaggttg gcaggacaaa cgtttgggac atttaatcca
     1201 aaaggtgata gacaaaccgg aagacgaatg gaatgtcgac aaaatggtgg cggctgccaa
     1261 tatgtcgcgc gcgcaactga tgcgccgttt caaaagccgg gtcggactca gcccgcacgc
     1321 ctttgtgaac catatccgcc tgcaaaaagg cgcgttgctg ctgaaaaaaa acccggattc
     1381 ggttttgtcg gtcgcactgt cggtaggctt tcagtcggaa acgcacttcg gcaaggcgtt
     1441 caaacggcaa tatcacgttt cgccgggtca ataccggaaa gaaggcgggc aaaaataaat
     1501 cggggcttca aacgcaaatg ccgtctgaaa aggctttcat acagcatttg cgtaccgcgt
     1561 catttcaagg gctgcatctt catcacttcc atcaaaaagt tggtaaatgc ggggttgttg
     1621 ggtttgacat ccatattttt ccaacgctgc tgccagccgc gcaaggcatt ctggatatac
     1681 agcttggact gttccgtatt gattgcgccc cgctggctgt ctatcgccga acgcaggtag
     1741 atttcataca tactgtcatc gacggcattg cgtccgacca ggcgttttct gaagttgttc
     1801 agatattgcg ccgcctgaac cttggtcatt ttaccgatac ccacctgata gcccaagcgc
     1861 gtcgcttcat cgctgatttt ggcaacatcc gtccaatgcg aagaggcaag gcggaaacct
     1921 tttgcaggtg cttccgtttt gacggtattg ataggattca cggggatttc cgtcaatgtg
     1981 ggcacataaa tagactggca gccggaaaga actgccacaa tggaaagagg gataaggtat
     2041 tttttcatgc ccccattata atcaagtttg ccttgagaaa acaaattgtt cggcaagaaa
     2101 aataaaattt cggcatcaga agcaggcaaa aacacattcc acaagccttg ccgcaaggtt
     2161 tacaatccga ccgtccttat cgcaacgacc gtttatggat accgcaaaaa aagacatttt
     2221 aggatcgggc tggatgctgg tggcggcggc ctgctttacc attatgaacg tattgattaa
     2281 agaggcatcg gcaaaatttg ccctcggcag cggcgaattg gtcttttggc gcatgctgtt
     2341 ttcaaccgtt gcgctcgggg ctgccgccgt attgcgtcgg gacaccttcc gcacgcccca
     2401 ttggaaaaac cacttaaacc gcagtatggt cgggacgggg gcgatgctgc tgctgtttta
     2461 cgcggtaacg catctgcctt tggccaccgg cgttaccctg agttacacct cgtcgatttt
     2521 tttggcggta ttttccttcc tgattttgaa agaacggatt tccgtttaca cgcaggcggt
     2581 gctgctcctt ggttttgccg gcgtggtatt gctgcttaat ccctcgttcc gcagcggtca
     2641 ggaaacggcg gcactcgccg ggctggcggg cggcgcgatg tccggctggg cgtatttgaa
     2701 agtgcgcgaa ctgtctttgg cgggcgaacc cggctggcgc gtcgtgtttt acctttccgt
     2761 gacaggtgtg gcgatgtcgt cggtttgggc gacgctgacc ggctggcaca ccctgtcctt
     2821 tccatcggca gtttatctgt cgtgcatcgg cgtgtccgcg ctgattgccc aactgtcgat
     2881 gacgcgcgcc tacaaagtcg gcgacaaatt cacggttgcc tcgctttcct atatgaccgt
     2941 cgttttttcc gccctgtctg ccgcattttt tctgggcgaa gagcttttct ggcaggaaat
     3001 actcggtatg tgcatcatca tcctcagcgg tattttgagc agcatccgcc ccactgcctt
     3061 caaacagcgg ctgcaatccc tgttccgcca aagataaaaa atgccgtccg aacatccttc
     3121 agacggcata tcgggcttta tttccccgcc ttcacatcct gccactggcg caccataaac
     3181 ttcaatgccg ccggctggat aggcaccatg ataaagctgt ttttcaaatc ctcctcggtt
     3241 gggaaaatcg tattgtcgtt tttaaattcg tcttccatca gctcacgcgc cggcttgctc
     3301 gaaggcgcat aagtaacgaa attgccgttt ttcgccgaca cttccgggtc gaggaagtcg
     3361 ttgatgtatt tgtgcgcgtt ggcgacgttt ttcgcatctt tcggaatcac gaaagaatcc
     3421 acccaaatcc ccacgccctc tttcggcatc atcacgcgga ttttttcctt gccgcccgct
     3481 tcttcggcac ggcgtttggc gatgttcaaa tcgccgccga aaccgattgt tacgcaggta
     3541 tcgccgcgcg ccaaatcatc gataaagccg gacgaagtaa agcgtttgat attggggcgg
     3601 tttttcttga gtagggcggt tgcctccctg atgtcttccg tattgctgct gttcgggttt
     3661 ttacccaaat agttcaacac cataggatag atttccgccg cgctgtccaa atagctgatg
     3721 ccgcattgct tgagtttgga cgtgtattcg gggtcgaaca ccaaatccca ctggttgtcc
     3781 ggcagcttgt ccgtacccaa agcctttttc acgcgttcgg tattgatggc gaaggtattt
     3841 gtcccccaat aaaacggcac ggcgtattcg tggccgggat cgaccccgtc catcagcctc
     3901 atcatttcgg gattgaggtg tttataattg ggaatcagcg atttatcgat tttctgatac
     3961 gcacctgcct taatctgcct gcccacaaac gcattggacg gcgcgacaat gtcgtaaccg
     4021 gattttccgg tcagcacctt gctttccagc gtttcatcgc tgtcgtacac atcataagta
     4081 accttgatgc cgtttttctt ttcaaaatcg gcaacggttt ccggatcgac gtattccgac
     4141 cagttgtaaa ttttcaatac gtttcggttt tccgccggcg ccggtttttc ggcaggcggt
     4201 ttgtccgaac cgccgcacgc tgcaagcagc aaagcagtca ggacggcgag tggcagatgt
     4261 ttggccatta tcattccttg catatcgggt tggagaaagc ggccattata gccgatattg
     4321 ggaacagggc ttcagacggc attcaaaatc ccgcaatgcc gtctgaaagc cgccgcttcc
     4381 atagctagaa acagggattt gcggtaagat accgccgttc gttttccctg cttttaccat
     4441 gacaagacat ttgagagaca ttgaaaaaat tatgaaaacc tccgaactgc gccaaaaatt
     4501 cctaaaattt tttgaaacca aaggccacac cgtcgtccgc tcttccagcc tcgtgccgca
     4561 cgacgacccg accctgctgt ttaccaacgc gggcatgaac cagtttaaag acgtattctt
     4621 aggtttcgac aaacgcccgt acagccgcgc caccaccgcg caaaaatgcg tacgcgcagg
     4681 cggcaagcac aacgacttgg aaaacgtcgg ctacaccgcc cgccaccaca ccttctttga
     4741 aatgatgggc aacttctcct tcggcgacta cttcaaacgc gacgccatcc acttcgcttg
     4801 ggaatttctg acttcccccg aatggctcaa catccctaaa gacaaactgt tggcgaccgt
     4861 ttacgcggaa gacgacgaag cctacaacat ctggttgaac gaaatcggta tgccgtccga
     4921 gcgcatcgtc cgcatcggcg acaacaaagg cgcgaaatac gtgtccgaca acttctggca
     4981 aatgggcgac accggccctt gcggcccctg ctccgaaatt ttctacgacc acggcgaaga
     5041 aatctggggc ggcattcccg gcagccccga agaagacggc gaccgctgga tcgaaatttg
     5101 gaactgcgta tttatgcagt tcaaccgcga cgaacaaggc aatatgaatc cgctgcccaa
     5161 gccgtccgtc gataccggta tgggcttgga acgtatggcc gccgtcatgc agcatgttca
     5221 cagcaactac gaaatcgact tgttccaaga cctgctcaaa gccgttgccc gcgaaaccgg
     5281 cgcagctttc agcatggatg aacccagcct gaaagtcatc gccgaccaca tccgctcctg
     5341 ctcgttcttg attgcagacg gcgtgttgcc ttccaacgaa ggccgcggct acgtattgcg
     5401 ccgcatcatc cgccgcgccg tgcgccacgg ttacaaactg ggtcaaagca aaccgttctt
     5461 ccacaaactg gtggccgatt tggtcaaaga gatgggcgat gcctaccctg aattgaaaga
     5521 aaaacaagtt caaatcgaag aagcgttgaa aaacgaagaa agccgttttg cccaaacgct
     5581 ggaaaccggt atggctttgt tggaaaacgc gttggctaaa ggcggcaaaa cactcgacgg
     5641 cgaaatcatc ttcaaactct acgatactta cggtttccca tacgacttga ctgccgacat
     5701 ctgccgcgaa cgcaatatcg aactggacga agcaggcttc gagcgcgaaa tggaagcaca
     5761 acgcgcacgt gcacgcgccg cacaaagctt caaagccaac gcccaactgc cttacgacgg
     5821 tcaggacacc gagtttaaag gttatagcga acgtcaaacc gaatccaaag tcctcgccct
     5881 ctacaaagac ggcgagcaag tcaacgaatt gaacgaaggc gacagcggcg cagtcgtcat
     5941 cgactttacc ccgttctatg cagaatccgg cggccaagtc ggcgatgtcg gctatatctt
     6001 ctcaggcgaa aaccgctttg aagtacgcga tacccaaaaa atcaaagcgg ccgtattcgg
     6061 tcaattcggc gtacaaactt caggccgtct gaaagtcggc gacagcgtta ccgccaaagt
     6121 ggacgacgaa atccgcaatg ccaatatgcg caaccacagc gcaacccact tgatgcacaa
     6181 agccctgcgc gatgtattgg gcagacacgt cgaacaaaaa ggctctttgg ttaccgccga
     6241 atccacccgt ttcgacattt cccatcccca agcggtaact gccgaagaaa tcgccgaagt
     6301 cgaacgccgc gtcaacgaag ccgttttggc gaacgttgcc gtcaacgccg ccattatgag
     6361 catggaagac gcgcaaaaaa ccggcgccat gatgctcttc ggcgaaaaat atggcgacga
     6421 agtacgcgta ttgcaaatgg gcggtttctc taccgaattg tgcggcggca cgcacgtttc
     6481 acgcaccggc gacatcggcc tcttcaaaat catcagcgaa ggcggtattg ccgcaggcgt
     6541 gcgccgtatc gaagccatca ccggcctgaa cgcactcaaa tgggcgcaag agcaagagcg
     6601 tttggtgaaa gacatcattg ctgaaaccaa agcccaaacc gaaaaagacg tactggcgaa
     6661 aatccaagca ggcgcggcac acgccaaagc attggaaaaa gaattggcac gcgccaaagc
     6721 cgaactcgcc gtccacgcag gcgcaaaact cttggacgac gcaaaagact tgggcgcagc
     6781 caaactcgtt gccgcccaaa tcgaagccga cgcagccgcc ctgcgcgaaa tcgtaaccga
     6841 cctgaccgga aaatccgaca acgccgtgat tcttttagcg gcagtaaacg acggcaaagt
     6901 ctccctgtgc gccggcgtat ccaaaccgtt gaccggcaaa gtgaaagcag gcgatctggt
     6961 taaatttgca gccgaacaag tcggcggcaa aggaggcggc cgtcccgact tggcgcaggc
     7021 cggcggcacg gatgccggca aattgcccga aatgctggta agcgtggaaa gctggctttg
     7081 tcagaaatta agttaaattt aaatcttaat aagccatttg agtcttgttc aaatggctta
     7141 tattttggat tgttagaatg aatactaaaa cagagttaca aaaactttta gaagaggata
     7201 tatcaacttt aacagaaaca ctaatttgtg cagatgctct tcctccaaga tatgtacgta
     7261 gcattgcaac tcctattgtc cgtcgatggt taattgacaa acaattaaat attctcgcaa
     7321 aagaaatagg tttaactata gaattgccca tccttgatac aagtttggta tttgaaaaat
     7381 tgtcaacctt agagaataaa gttaattttt atatggctgg tggagtttat ttaggcggag
     7441 aatttattag ctctatatat cattcatcac aagaattttc tggtgaacca ataatctatg
     7501 ctgaaccaaa tataatttta tgtcctgctg aaaaattttt aactttaaaa cgagttttcc
     7561 ataatggaaa tatttttaat atgaatcaga taattacttt tttatccaat aaacaaggag
     7621 gagtacattt tgataaaaat tacgataaat ataaaacttg gcaagttgct atagaaaaag
     7681 cagcaaattt tttaaaactc ggtaatccat ataatgaaga taaattatct cttagtgaag
     7741 aacatgatac tattttagta gttttaccat tagaaaaagg atatgaatgg aattgtcttg
     7801 agatagaagt actatccgct gctcaatctc tagcaaatat ctattgcaac aaagttcgcc
     7861 tcatagatgg tcatgtttgg aaggaataaa agaaaaagca tagttggtaa tttttagtta
     7921 cgtagggtgg gcttcagccc accgtttttt catttttgat tgttgattgg tgggctaaag
     7981 cccaccctac ggttactaaa atcccgtcat tacctcgcag gtgggaatcc ggtttgttcg
     8041 gtttcggttt tttagggaca gtgccgcaac attaaatttc cagattccca ctttcgcaca
     8101 aataacagcg caaatagggc aaagataagg atatgggttt ttttctttga atttactata
     8161 caattcgccc gtccccgtta tagaaaccca agccgatgaa aaccctgctc caactccaaa
     8221 ccgccgcaca aaacttcgcc gcctactaca aagaccaaac cgacgaacgc cgcgagaaag
     8281 acaccttcta gaacgaattt ttcgccattt tcggcatcga ccgcaaaaac gtcgcccact
     8341 tcgaataccc cgtcaaagac cctgccgaca acacccaatt cgtcgatata ttttgggaag
     8401 gcatcttcct tgccgaacac aaatccgcca acaaaaacct gaccaaggcc aaagagcagg
     8461 cggagcgtta tttgcaggaa atcgggcgca ccaagccctc tgcgctgccc gaatattacg
     8521 ccgtcagcga ttttgcccat ttccaccttt atcgccgcgt acctgaagaa ggcgtggaaa
     8581 accaatggca gttccctttg gaagcattgc ccgaatacat cacgcgcggc gttttcgact
     8641 tcatgttcgg catcgaagcc aaagtccgcc aaattcaaga agaagccgat attcaagcgg
     8701 cggcggccat cggcaggctg cacgacgcgc tcaaagaaga aggcatttac gaagaacacg
     8761 agctgcgcct cttcatcacg cgcctgcttt tcctcttttt tgccgacgac agcgccgttt
     8821 tccagcgcaa ctatctcttt caagactttt tagaaagctg caaagaaacc gacacgctcg
     8881 gcgacaagct caaccaactc tttgaattcc tcaacacacc cgaccaaaag cgcagcaaga
     8941 cccaaagcga aaaatttaaa ggtttcgaat acgtcaacgg cggcctgttt aaagaacgcc
     9001 tgcgcacctt cgacttcacc gccaagcagc accgcgcctt aatcgactgc ggcaatttcg
     9061 actggcgcaa
//
LOCUS       DAXHSF010000073         8936 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.073, whole genome shotgun sequence.
ACCESSION   DAXHSF010000073 DAXHSF010000000
VERSION     DAXHSF010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8936
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.073"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..193
                     /locus_tag="ACFM8S_001210"
     CDS             <1..193
                     /locus_tag="ACFM8S_001210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314284.1"
                     /translation="KFRRCFQIARSNSRASAGLFEIKGLELNAKRLNHYCPMRQTGIV
                     RQYGCLKIGKSGWKTKGRT"
     gene            169..1092
                     /locus_tag="ACFM8S_001211"
     CDS             169..1092
                     /locus_tag="ACFM8S_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFC6314285.1"
                     /translation="MENQRPHLGFALALLAAMTWGTLPIAVRQVLKFVDAPTLVWVRF
                     TVAAAVLFVLLALGGRLPKRRDFSWCSFRLLLLGVAGISANFVLIAQGLHYISPTTTQ
                     VLWQISPFTMIVVGVLVFKDRMTAAQKIGLVLLLAGLLMFFNDKFGELSGLGAYAKGV
                     LLCAAGSMAWVCYAVAQKLLSAQFGPQQILLLIYAASAAVFLPFAKPAHIGSLDGTLA
                     WVCFAYCCLNTLIGYGSFGEALKHWEASKVSAVTTLLPVFTVIFSLLGHYVMPDTFAA
                     PDMNGLGYAGALVVVGGAVTAAVGDRLFKRR"
     gene            complement(1157..2545)
                     /gene="fumC"
                     /locus_tag="ACFM8S_001212"
     CDS             complement(1157..2545)
                     /gene="fumC"
                     /locus_tag="ACFM8S_001212"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFC6314286.1"
                     /translation="MSTRTEHDTMGNVEVPSEAYWGAQTQRSRNNFKIGGETLPQPLI
                     YALALVKKAAAATNVSLGRIKPEQADLITQAADDVLNGKLDGQFPLVVWQTGSGTQSN
                     MNMNEVLANRANEIAGTGLAAYQPVHPNDHVNHAQSTNDAFPTAIHVAAAIEINRHLI
                     PAVKALRDTLDKKAQAFAPIVKIGRTHLQDATPLTLGQEFSGYVSQLDHGLGRLNDAL
                     KDLYELALGGTAVGTGLNSHPEYAEKAAAKLAELSGLPFVSAPNKFEALGGRDAAVAA
                     SGALKTLAASLNKIANDIRWLASGPRCGLGEIKIPENEPGSSIMPGKVNPTQCEAMTM
                     VCCQVFGNDVTIGMAGASGNFELNVYMPVIAYNLLQSIRLLGDACNSFNEHCAIGIEP
                     VPEKIDYFLHHSLMLVTALNRKIGYENAAKVAKTAYKNNKSLRETAVELGLLTGEEFD
                     ELVVPADMVHPR"
     gene            complement(2689..4668)
                     /gene="tkt"
                     /locus_tag="ACFM8S_001213"
     CDS             complement(2689..4668)
                     /gene="tkt"
                     /locus_tag="ACFM8S_001213"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFC6314287.1"
                     /translation="MSQLANAIRFLSADAVQKANSGHPGAPMGMAEMAETLWTKFLNH
                     NPANPKFYNRDRFVLSNGHASMLLYSLLHLTGYNLSIEDLKNFRQLHSKTPGHPEYGY
                     TDGVETTTGPLGQGIANAVGMALAEKILAAEFNKDGLNIVDHYTYVFMGDGCLMEGVS
                     HEACSLAGTLGLGKLIVLYDDNNISIDGKVDGWFTENIPQRFESYGWHVVPNVNGHDT
                     AAIQAAIEAARAETGKPSIICCKTLIGKGSANKEGSHKTHGAPLGADEIEATRKHLGW
                     TYPAFEIPQEIYDAWNAKEQGAKLEADWNELFAQYQAKYPAEAAEFVRRMDKKLPDNF
                     DEYVQTALKEVCAKAETIATRKASQNSIEILAKELPELVGGSADLTPSNLTDWSNSVS
                     VTRDKGGNYIHYGVREFGMGAIMNGLALHGGIKPFGATFLMFSEYERNALRMAALMKI
                     NPVFVFTHDSIGLGEDGPTHQPIEQTATLRLIPNMDVWRPCDTAESLVAWAEAVKAAD
                     HPSCLIFSRQNLKFQARSEQQLDDIKRGGYIISEAQGNAQAVIIATGSEVELALEAQK
                     ALAAQNIAVRVVSMPSTNVFDRQDAAYQAAVLPESLPRIAVEAGHADGWYKYVGLNGA
                     VVGINRFGESAPADLLFKAFGFTVDNVVDTVKSVL"
     gene            5195..6691
                     /gene="ccoG"
                     /locus_tag="ACFM8S_001214"
     CDS             5195..6691
                     /gene="ccoG"
                     /locus_tag="ACFM8S_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HFC6314288.1"
                     /translation="MTTENQAGSPASGIGTSEQTKAAPKAKKTFDPRASVIQIHPEGE
                     RIHPKKAEGRFAKLRIAAVLATQFVFYVIPWFNWSGRQAVVFNIPERHFFIFGLSLGV
                     GDLIYLALLLMICAFGLFWWTTIAGRLWCGYSCPQTVYTEIMLWIDNLVEGDRNKRLK
                     LEKSPWNFTKIRIKATKYLLIFLVCAWTGITFAGWFVPIRQFVPDLFTGAAGGGAMFA
                     AAFYGFMTFFFAHIMREKVCLHMCPYARFQSAMFDKDTLIVSYDAERGEPRGARKKTV
                     NKEEAGLGDCINCAMCVQVCPVGIDIRNGLQYQCIGCAACIDACDEIMDKMGYPRGLI
                     RYTTESALEHEYSEKDIKKRLLRPRVAGYGAVLALVIIAFLAGLSTRKMVEVDILKDR
                     GVLVRENAKGWLENAYSLRIINKSEKEQLITASVKGFDEIALTGLPEGGIKVAPRETV
                     TLPVQVSTIPEYADKGSHPIEFTFQYRESGAPDGKPVVLEEDATFIGE"
     gene            6694..7242
                     /locus_tag="ACFM8S_001215"
     CDS             6694..7242
                     /locus_tag="ACFM8S_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HFC6314289.1"
                     /translation="MSQNTPIKPWYKHVWPWILMAGPIFVVIASVAMFFVAQQHATDL
                     VTDDYYKDGKHIDIQLHRDEEAVRRHIGVQVLISPDMNAAKVFVGGEFDGKQPLNLLL
                     MHPTRKADDQTVALKPVGSAQNGRAEYEAVFKTLSPTNHWYVRVEDAAGVWRVENKWI
                     TSQGNAVDLTPMDKLFNNAGSK"
     gene            complement(7365..7553)
                     /locus_tag="ACFM8S_001216"
     CDS             complement(7365..7553)
                     /locus_tag="ACFM8S_001216"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314290.1"
                     /translation="MPSEAFQTAFFRLRFGIVSKKRREIKTFLCKQNAFRIVSGIQHK
                     CRLKPFRRHFSGCISEWC"
     gene            complement(7583..8758)
                     /locus_tag="ACFM8S_001217"
     CDS             complement(7583..8758)
                     /locus_tag="ACFM8S_001217"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HFC6314291.1"
                     /translation="MQNIFDPLVIRGKSLTPIVQGGMGVGVSASGLSSAVARENGIGT
                     IASVDLRHLHEDLLAESQINPSEEKYTSLNCTALDREIQKAKSASEGKGLIAVNVMKA
                     VKDHAAYVRQACESGADAVVMGAGLPLDLPEMTEGYHKDVALLPILSESRGINIVLKR
                     WMKKGILPDAIVVEHPAHAAGHLGASTVEGVNDAKFDFKRVIEETFEVFKSLGLESEK
                     IPLILAGGMANFEKVKTALKNWGASAVQIGTAFAVTEEGDAHLNFKKTLAGAETEKVV
                     EFMSVAGLPARGVRTKFLDSYIKRESKLQANAKADPRRCTQGLNCLTSCGLRDGLSKA
                     GQFCIDIQLAAAFRGEVDKGLFFRGKDPLPFGNAIRTVRETIQYLLTGSEPVATLGR"
BASE COUNT         1927 a         2179 c         2577 g         2253 t
ORIGIN      
        1 aaaattcagg cggtgttttc aaatagcgcg aagcaatagc cgagcttcag ccggtttgtt
       61 tgaaatcaaa gggttggaat taaacgccaa acgcttgaac cattattgcc cgatgcggca
      121 aaccggcatc gtccggcaat acggttgttt aaaaatagga aaatcaggat ggaaaaccaa
      181 aggccgcacc taggcttcgc gttggcactt ttggcggcga tgacgtgggg aacgctgccg
      241 attgccgtgc ggcaggtatt gaagtttgtc gatgcgccga cgctggtgtg ggtgcgtttt
      301 accgtggcgg cggcggtatt gtttgttttg ctggcattgg gcgggcggct gccgaagcgg
      361 cgggattttt cttggtgctc attcaggctg ctgctgctcg gcgtggcggg catttcggca
      421 aactttgtgc tgattgccca agggctgcat tatatttcgc cgaccacgac gcaggttttg
      481 tggcagattt cgccgtttac gatgattgtt gtcggcgtgt tggtgtttaa agaccggatg
      541 actgccgctc agaaaatcgg cttggttttg ctgcttgccg gtttgcttat gttttttaac
      601 gataaattcg gcgagttgtc gggtttgggc gcgtatgcga agggcgtgtt gctgtgtgcg
      661 gcaggcagta tggcatgggt gtgttatgcc gtggcgcaaa agctgctgtc ggcgcaattc
      721 ggaccgcaac agattctgct gttgatttat gcggcaagtg ccgccgtgtt cctgccgttt
      781 gccaaaccgg cacacatcgg aagtttggac ggtacgttgg cgtgggtttg ttttgcgtat
      841 tgctgcttga atacgttaat cggttacggc tcgttcggcg aggcgttgaa acattgggag
      901 gcttccaaag tcagcgcggt aacaaccttg ctccccgtgt ttaccgtaat attttctttg
      961 ctcgggcatt atgtgatgcc tgatactttt gccgcgccgg atatgaacgg tttgggttat
     1021 gccggcgcac tggtcgtggt cgggggtgcg gttacggcgg cggtggggga caggctgttc
     1081 aaacgccgct agttcgcagg caacggaaaa tgccgtccga acgaggtttc agacggcatt
     1141 ttatttgagg gaaagattag cgcggatgaa ccatatcggc aggaacgacc agttcgtcaa
     1201 attcttcgcc cgtcagcaag cccaactcaa cggcggtttc gcgcaacgat ttgttgtttt
     1261 tgtaggcggt tttggcgact ttggcggcgt tttcgtaacc gattttacgg tttaacgcgg
     1321 taacgagcat cagggaatgg tgcaggaaat agtcgatttt ttccggtacg ggttcgatgc
     1381 cgatggcgca gtgttcgttg aagctgttgc acgcgtcgcc caagaggcgg atggattgca
     1441 agaggttgta ggcgataacg ggcatataga cgttcagctc gaaattgccc gacgcgcccg
     1501 ccatgccgat ggtaacgtcg ttgccgaaca cttggcagca caccatggtc atcgcttcgc
     1561 attgggtcgg gttgactttg cccggcatga tggacgaacc cggctcgttt tcggggattt
     1621 tgatttcgcc caaaccgcag cgcgggccgc ttgccagcca gcggatgtcg ttggcgattt
     1681 tattcaggct tgccgccagc gttttcaatg cgcccgaagc ggcaacggcg gcatcgcgtc
     1741 cgcccaaggc ttcaaatttg ttcggcgcgc tgacaaacgg caagccggac aattcggcga
     1801 gtttggcggc ggctttttcg gcgtattcgg gatggctgtt caaacccgtg ccgaccgccg
     1861 taccgcccaa agcaagttca tacaagtctt taagcgcgtc gttcagacgg cctaagccgt
     1921 ggtcgagctg ggaaacgtag ccggaaaatt cttggcctaa agtcagcggc gtcgcgtcct
     1981 gcaagtgggt gcggccgatt ttgacgatag gggcgaaggc ttgggctttt ttgtccaagg
     2041 tgtcgcgcag ggcttttacg gcggggatga ggtggcggtt gatttcaatc gcggcggcaa
     2101 cgtggatggc ggtcgggaat gcgtcgttgg tcgattgcgc gtggttcaca tggtcgttgg
     2161 gatggacggg ctgatacgcc gccaaacccg taccggcgat ttcgttggcg cggttcgcca
     2221 gcacttcgtt catgttcata ttggactgcg tgccggagcc ggtctgccaa accactaaag
     2281 gaaactgtcc gtcgagcttg ccgttcaaca catcatccgc cgcctgcgta atcaaatccg
     2341 cctgttccgg cttaatcctg ccgagggaaa cattggtcgc ggcggcggct tttttcacca
     2401 gcgccaaagc ataaatcaac ggctgcggca gggtttcgcc gccgattttg aaattgttgc
     2461 ggctgcgctg ggtctgcgcg ccccaatagg cttcggacgg gacttcgaca ttgcccatgg
     2521 tgtcgtgttc ggtacgggtg ctcatgcgtt tctccttttg aaatgtgaat aagagtgatt
     2581 cgcaaatatt ataatggaga ttggcggaat gaggaagcat taaggctgga gggcaaaaaa
     2641 atgccgtctg aaaagcgttc agacggcatt tgatgcggcg gctgcggttt acagcacgga
     2701 tttcactgta tcgaccacat tgtccacggt aaagccgaat gctttgaaga gtaaatcggc
     2761 aggggcggat tcgccgaagc ggttgatgcc gacgactgcg ccgttcagtc cgacatattt
     2821 gtaccagccg tcggcgtggc cggcttctac ggcgatgcgc ggcaggcttt cgggcaggac
     2881 ggcggcttga taggcggcgt cttggcggtc gaatacgttg gtggacggca tggaaacgac
     2941 gcgcacggcg atgttttgcg cggcgagggc tttttgcgct tccaaagcca gctcgacttc
     3001 tgagccggtg gcaatgatga cggcttgggc gttgccttgg gcttcgctga tgatgtagcc
     3061 gccgcgtttg atgtcgtcca gttgttgttc gctgcgcgct tggaatttca ggttttgacg
     3121 gctgaaaatc aggcaggacg ggtgatcggc ggctttgacg gcttctgccc aagcgaccaa
     3181 ggattcggcg gtgtcgcacg gccgccatac gtccatattc ggaatcaggc gcagggtggc
     3241 ggtttgctcg atgggttggt gggtcggacc gtcttcgccc aaaccgatgg aatcgtgggt
     3301 gaacacaaat acagggttga ttttcatcag tgccgccata cgcagggcgt tgcgctcgta
     3361 ttcgctgaac atcaggaagg tcgcaccgaa tggtttgatg ccgccgtgca gggcaagacc
     3421 gttcataatc gcacccatac cgaactcgcg cacgccgtag tggatgtagt tgccgccttt
     3481 gtcgcgggta acggagacgc tgtttgacca gtcggtcagg ttggacgggg tcaggtcggc
     3541 cgaaccgccc accaattcag gcaactcttt tgccaagatt tcgatgctgt tttggctggc
     3601 tttgcgggta gcgatggttt cggctttggc gcacacttct ttcaatgccg tctgaacgta
     3661 ttcatcgaaa ttgtccggca gctttttatc catacggcgc acaaattctg cggcttcggc
     3721 aggatatttg gcttgatatt gcgcgaacag ttcgttccag tcggcttcca gtttcgcgcc
     3781 ttgttctttg gcattccacg catcgtaaat ttcttgcggg atttcaaagg cggggtaagt
     3841 ccagcccaaa tgtttgcgcg tggcttcgat ttcgtccgcg cccaaaggtg cgccgtgggt
     3901 tttgtggctg ccttctttgt tggcactgcc tttgccgatt aaggttttgc agcagatgat
     3961 ggacggtttg ccggtttcgg cacgtgcggc ttcgatggcg gcttgaatgg cggcggtgtc
     4021 atgaccgttt acgttaggaa cgacgtgcca gccgtagctt tcaaagcgtt gcgggatgtt
     4081 ttcggtaaac cagccgtcca ctttaccatc aatggaaata ttgttatcgt catacaaaac
     4141 aatcagtttg cccaagccta aagtgccggc gagcgaacag gcttcgtgcg atacgccttc
     4201 catcagacag ccgtcgccca tgaagacgta ggtgtaatga tcgacgatgt tcaaaccgtc
     4261 tttattaaat tcggcggcaa ggattttttc tgccaatgcc atacccaccg cgttggcaat
     4321 cccttgcccc aacgggccgg tcgtggtttc cacgccgtcg gtgtagccgt attcggggtg
     4381 gccgggggtt ttgctgtgca gctggcggaa gtttttcaag tcttcaatgc tcagattgta
     4441 gccggtcagg tgcagcaggc tgtacaacag catagacgcg tggccgttgg agaggacgaa
     4501 gcggtcgcgg ttgtagaatt tggggttggc ggggttgtga ttgaggaatt tcgtccacaa
     4561 tgtttccgcc atttccgcca tacccatagg cgcgccgggg tggccggaat tggctttttg
     4621 aacggcatcg gccgagagga agcggattgc gtttgccagt tgagacattt tgtattttcc
     4681 ttgctggtgt ttcagataag tggataatcg gaaagcgttg attatcgccc gattcgctta
     4741 tgctttcaag aaaagggcgg acgcgtggga aggcggcggc aggggacagg cggggaagga
     4801 ttttcgattt gcgggcgaag cctgccatta ttccttttga aataaaaagt tatagattgt
     4861 gtgccggatt gtcgatagcg tttgtttatg agcttgcgcc gtcggttctg ccgatatggg
     4921 ggtgtcggtt tttttagtct tttttttaac cgtattcgga ttttgttcgg gcggtaaggt
     4981 aaaatccagg cgttttgatg cggatgggaa atgtatccgc cccatacatc cggacggcgc
     5041 ataaagttgt acaatagcgg aaatatattt tgggaaaaac ggattttctc aaaacttgaa
     5101 acacaacgcc ttaaaaaaca agaaaaatga cgtctgaaaa gcaaacggcg gcggtaacgg
     5161 gcaaaccatc aatcaaaaat ctaaggaatg cagaatgacc acggaaaacc aagccggcag
     5221 tccggcatcc ggaatcggca cgtccgaaca aacaaaagcc gcgccgaaag caaaaaaaac
     5281 attcgatccg cgagccagcg tgattcaaat ccatcccgaa ggcgaacgca tccatcccaa
     5341 aaaggcggaa ggacggttcg ccaaactgcg tatcgccgcc gtattggcga cgcagtttgt
     5401 gttttacgtc attccgtggt tcaactggag cggcaggcag gccgtcgttt tcaatatccc
     5461 cgaacggcat ttcttcattt tcggattgtc gttgggggtg ggcgatttga tttaccttgc
     5521 cttgctgctg atgatttgcg ccttcgggct gttttggtgg acgacgattg cagggcgact
     5581 gtggtgcggc tattcctgcc cgcaaacggt ttacaccgaa attatgctgt ggattgacaa
     5641 cctggtcgaa ggcgatagaa acaaacggct gaaactggaa aaatcgccgt ggaatttcac
     5701 taaaatccgc atcaaagcca ccaaatacct gctgattttc cttgtctgcg cgtggacggg
     5761 catcacgttt gcaggctggt ttgtccctat ccgccagttc gttcccgatt tattcactgg
     5821 agcagcaggt ggcggcgcga tgtttgccgc agcgttttat ggctttatga ccttcttctt
     5881 cgcccacatt atgcgtgaaa aagtatgcct gcatatgtgt ccgtatgcac gtttccaaag
     5941 cgcgatgttc gacaaggaca cgctgattgt ttcttatgac gcggaacgcg gcgaaccgcg
     6001 cggcgcgcgc aagaaaacgg tcaataagga agaggcgggt ttgggcgact gcatcaactg
     6061 tgcgatgtgc gtccaagtct gccccgtcgg catcgacatc cgcaacggtc tgcaatacca
     6121 atgtatcggc tgcgccgcct gtatcgacgc gtgcgatgag attatggaca aaatgggcta
     6181 tccgcgcgga ttaatccgtt atacgaccga aagcgcgctg gaacacgaat attctgaaaa
     6241 agacattaaa aaacggctgc tcagaccgcg cgtggcaggt tacggcgcgg tgttggcgtt
     6301 agttatcatt gccttcctgg ccggtttgtc cacgcgcaaa atggtcgagg tcgatatttt
     6361 gaaagaccgt ggcgtactgg tgcgcgaaaa cgccaaaggc tggctggaaa acgcatacag
     6421 cctgcgtatc atcaacaaaa gtgaaaaaga acagctcatt accgcaagtg tcaaaggctt
     6481 tgacgaaatc gccctgaccg ggctgcccga aggcggtatc aaggttgccc cgcgcgaaac
     6541 ggtaaccctt cccgtccaag tgtccaccat tccggaatac gcggacaaag gcagccaccc
     6601 tatcgaattt accttccaat accgcgaaag cggcgcgccc gacggcaagc cggtcgtctt
     6661 ggaagaagat gcaaccttta tcggagaata accgtgtctc aaaacactcc aatcaaacct
     6721 tggtacaaac acgtctggcc gtggatcttg atggcggggc cgatttttgt cgtcatcgcc
     6781 agcgtcgcta tgttttttgt cgcgcagcag cacgcgacag atttggttac ggacgattat
     6841 tataaagacg gcaaacatat cgacatccag cttcatcggg atgaagaagc cgtcagacgg
     6901 catatcgggg tgcaggttct catttccccc gatatgaatg cggcaaaagt gtttgtcggc
     6961 ggcgagtttg acggcaaaca gcctttgaac ctgctgctga tgcacccgac ccgcaaggcg
     7021 gacgatcaaa ccgtcgccct caagcccgtc ggcagcgcgc agaacggcag ggcggaatat
     7081 gaggcggtgt tcaaaaccct ttcgccgacc aaccactggt atgtgcgcgt ggaggacgcg
     7141 gcaggcgtgt ggcgcgtcga gaacaaatgg attaccagcc aaggcaatgc ggtggatctg
     7201 accccgatgg ataaactttt caataatgcc ggaagcaaat aaaaaagccg tgttttcatt
     7261 gcattggttt gccagatgca atgtcgggca ggcattgttt gattattcgg cgtgatggtc
     7321 tgatttatcg gacgaaaata aatgtatctt atcccgttta ttttttaaca ccattccgaa
     7381 atgcagcctg aaaaatgccg tctgaagggc ttcagacggc atttgtgttg tatgcccgac
     7441 acaatgcgga aggcgttttg tttacataaa aacgttttta tttctctccg ttttttcgac
     7501 actattccga aacgcagcct gaaaaatgcc gtctgaaagg cttcagacgg cattttgttt
     7561 gcaaatcaaa tcctacctga tgtcagcgtc cgagcgttgc aacaggttcg ctccccgtca
     7621 gcagatattg tatcgtctcg cggacggtgc ggatggcatt gccgaagggc agcgggtctt
     7681 tacctctgaa gaacaggcct ttatctactt ctccacggaa tgcggcggca agctggatat
     7741 caatgcagaa ttgtccggct ttggaaagcc cgtcgcgcag accgcaactg gtcaggcagt
     7801 ttaaaccttg ggtacagcgg cgcgggtcag ctttggcgtt tgcctgaagt ttgctttcac
     7861 gcttgatgta gctgtctagg aatttggtgc ggacaccgcg cgccggcaaa ccggcaacag
     7921 acataaattc aactactttt tcagtttccg caccggcgag cgtttttttg aagttaaggt
     7981 gtgcatctcc ttcttcggta acggcaaaag ccgtaccgat ttgaacggcg gatgctcccc
     8041 agttctttag ggcggttttg actttttcaa aatttgccat gcctcccgca agaataagcg
     8101 ggattttttc gctttccagc cctaaacttt tgaaaacttc aaacgtttcc tcaatcacgc
     8161 gtttgaagtc gaacttggca tcgtttacgc cttcaacggt tgatgcaccc aaatgtccgg
     8221 ccgcgtgggc aggatgttcg actacaatcg catcgggcaa tatgcctttt ttcatccaac
     8281 gtttcaagac gatattaata ccgcgcgatt cggacagaat cggcagcagc gcgacatctt
     8341 tatgatagcc ctcggtcatt tccggcaggt ctaaaggcag gccggcaccc attacaaccg
     8401 catccgcccc tgattcgcaa gcctggcgga catacgcggc gtggtctttg accgccttca
     8461 tcacgttgac cgcaatcagt ccttttccct ctgaagcgct tttggctttt tggatttccc
     8521 tgtctaatgc ggtacagttc aaagatgtat atttctcttc actcggattg atttgtgatt
     8581 cggcgagtag gtcttcgtga aggtggcgca aatccacact ggcaatcgtt ccgataccgt
     8641 tttcacgtgc caccgcgctg gataaacccg atgcggaaac accgaccccc ataccgcctt
     8701 gcacgatggg ggtaagggat tttccacgaa taaccaaagg gtcaaaaata ttttgcatca
     8761 gtttctccga gtatacgaat cagtcaggct catgaaaaat ggttctaact gttttagaac
     8821 tattgctcaa atttggtatt atactctaaa tatcgcggcg gtaaacaaga aagcagccgg
     8881 agcttcgggc atattctgtt acgcctgttt caatcgggag agaaaatatt atagtg
//
LOCUS       DAXHSF010000074         8812 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.074, whole genome shotgun sequence.
ACCESSION   DAXHSF010000074 DAXHSF010000000
VERSION     DAXHSF010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8812
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.074"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(375..650)
                     /locus_tag="ACFM8S_001218"
     CDS             complement(375..650)
                     /locus_tag="ACFM8S_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFC6314292.1"
                     /translation="MNASNWSVYLILCENSAFYCGISPNPQQRLAAHTTGKGAKYTRV
                     FKPVAMRIVAGGMDKGTALKQEIAVKKLTAAQKRKLWEQAEKMPSET"
     gene            complement(647..1930)
                     /locus_tag="ACFM8S_001219"
     CDS             complement(647..1930)
                     /locus_tag="ACFM8S_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HFC6314293.1"
                     /translation="MTASKSGFIGQIFSRNMLVCIFTGFTSGLPLYFLINLIPAWLRS
                     EQVDLKSIGLMALIGLPFTWKFLWSPLMDAVRLPVLGRRRGWMLLTQAGLLAALAVYA
                     FLNPRNHLPLIAGLSVLVAFFSASQDIVLDAFRREILSDEELGLGNSVHVNAYRIAAL
                     IPGSLSLVLADRMPWSEVFVITSLFMLPGLLMTLFLASEPVLPPAVPKTLKQTVVEPF
                     KEFFTRKGIASAVCVLLFIFLYKLGDSMATALATPFYLDMGFSKTDIGLIAKNAGLWP
                     AVAAGILGGVWMLKIGVNKALWLFGAVQAVTVLGFVWLAGFGHFDTVGTGERLMLAAV
                     IGAEAVGVGLGTAAFVSYMARETNPAFTATQLALFTSLSAVPRTVINSFAGYLIEWLG
                     YVPFFQLCFALALPGMLLLLKVAPWNGEKTQDAGR"
     gene            complement(2086..3504)
                     /gene="glnA"
                     /locus_tag="ACFM8S_001220"
     CDS             complement(2086..3504)
                     /gene="glnA"
                     /locus_tag="ACFM8S_001220"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFC6314294.1"
                     /translation="MSIKNAVKLIEESEARFVDLRFTDTKGKQHHFTVPARIVLEDPE
                     EWFENGQAFDGSSIGGWKGIQASDMQLRPDASTAFVDPFYDDVTVVITCDVIDPADGQ
                     GYDRDPRSIARRAEAYLKSSGIGDTAYFGPEPEFFVFDGVEFETDMHKTRYEITSESG
                     AWSSGLHLDGQNTGHRPTVKGGYAPVAPIDCGQDLRSAMVNILEELGIKVEVHHSEVG
                     TGSQMEIGTRFATLVKRADQTQDMKYVIQNVAHNFGKTATFMPKPIMGDNGSGMHVHQ
                     SIWKDGQNLFAGDGYAGLSDTALYYIGGIIKHAKALNAITNPSTNSYKRLVPHFEAPT
                     KLAYSAKNRSASIRIPSVNSSKARRIEARFPDPTANPYLAFAALLMAGLDGIQNKIHP
                     GDPADKNLYDLPPEEDALVPTVCASLEEALAALKADHEFLLRGGVFSKDWIDSYIAFK
                     EEDVRRIRMAPHPLEFEMYYSL"
     gene            3588..3737
                     /locus_tag="ACFM8S_001221"
     CDS             3588..3737
                     /locus_tag="ACFM8S_001221"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314295.1"
                     /translation="MPSETRPASNFKLENIVSDGKIVNNMLLFVCSKAEYPPFCPNAD
                     KTDAV"
     gene            complement(3778..4164)
                     /locus_tag="ACFM8S_001222"
     CDS             complement(3778..4164)
                     /locus_tag="ACFM8S_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HFC6314296.1"
                     /translation="MKKMLCLAVFALGLATPAVAADWVQVGIDSDNNTTFGDADSRTD
                     NRAWFETRFAKPQKYGNGKFFNTAKTLEEMDCSGKRFRILTATWYSKSGNSIASYTPS
                     YAEWNYVIPGTLGEAQYKFVCNGYPR"
     gene            4336..5145
                     /gene="aroE"
                     /locus_tag="ACFM8S_001223"
     CDS             4336..5145
                     /gene="aroE"
                     /locus_tag="ACFM8S_001223"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFC6314297.1"
                     /translation="MTALPRYAVFGNPVAHSKSPQIHQQFALQEGVDIEYERICADIG
                     GFAQAVSTFFETGGCGANVTVPFKQEAFHLADEHSERASAAGAVNTLILLKNGKLRGD
                     NTDGIGLANDITQVKNIAIEGKTILLLGAGGAVRGVIPVLKEHRPARIVIANRTHAKA
                     EELAQLFGIEAVPMADVNGGFDIIINGTSGGLSGQLPAVNPEIFRDCRLAYDMVYGEA
                     AKPFLDFARQSGAKQTADGLGMLVGQAAASYALWRGFTPNIRPVIEYMKAM"
     gene            5147..5848
                     /gene="mtgA"
                     /locus_tag="ACFM8S_001224"
     CDS             5147..5848
                     /gene="mtgA"
                     /locus_tag="ACFM8S_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFC6314298.1"
                     /translation="MFRIVKWLIALPVGIFIFFNAYVYGNIITYRAVAPHRTAFMSMR
                     MKQFEQEGRDVALDYRWMPYNRISVNLKKALIASEDARFAGHGGFDWGGIQNAIRRNR
                     NSGKVKAGGSTISQQLAKNLFLNESRSYIRKGEEAAITAMMEAVTDKDRIFELYLNSI
                     EWHYGVFGAEAASRYFYQIPAAKLSKQQAAKLTARVPAPLYYADHPKSKRLRNKTNIV
                     LRRMGSAELPESDTD"
     gene            complement(6051..6126)
                     /locus_tag="ACFM8S_001225"
     tRNA            complement(6051..6126)
                     /locus_tag="ACFM8S_001225"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6091..6093),aa:Phe,seq:gaa)
     gene            complement(6317..7051)
                     /gene="lptB"
                     /locus_tag="ACFM8S_001226"
     CDS             complement(6317..7051)
                     /gene="lptB"
                     /locus_tag="ACFM8S_001226"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFC6314299.1"
                     /translation="MSANVSRLVVQNLQKSFKKRQVVKSFSLEIESGEVVGLLGPNGA
                     GKTTSFYMIVGLIAADAGSVTLDGQELRHLPIHERARLGVGYLPQEASIFRKMTVEQN
                     IRAILEIRTKDKNQIDREIEKLLADLNIGHLRRSPAPSLSGGERRRVEIARVLVMKPH
                     FILLDEPFAGVDPIAVIDIQKIIGFLKSRGIGVLITDHNVRETLSICDRAYIISDGAV
                     LASGKPDDLVGNEQVRSVYLGKNFKY"
     gene            complement(7125..7655)
                     /gene="lptA"
                     /locus_tag="ACFM8S_001227"
     CDS             complement(7125..7655)
                     /gene="lptA"
                     /locus_tag="ACFM8S_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HFC6314300.1"
                     /translation="MIQKICKLFVLIAFFSASPAFALQSDSRQPIQIEADQGSLDQAN
                     QSTTFSGNVVIRQGTLNISAARVNVTRGGKGGESVRAEGSPVRFSQTLDGGKGTVRGQ
                     ANNVAYSSAGSTVVLTGNAKVQRGGDVAEGAVITYNTKTEVYTISGSTKSGAKSASKS
                     GRVSVVIQPSSTQKSE"
     gene            complement(7636..8217)
                     /gene="lptC"
                     /locus_tag="ACFM8S_001228"
     CDS             complement(7636..8217)
                     /gene="lptC"
                     /locus_tag="ACFM8S_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFC6314301.1"
                     /translation="MKVRWRYGIAFPLILAVALGSLSAWLGRISEVEIEEVRLNPDEP
                     QYTMDGLDGRRFDEQGYLKEHLSSKGAKQFPESSDIHFDSPHLVFFQEGRLLYEVGSD
                     EAVYHTENKQVLFKNNVVLTKTADGKRQAGKVEAEKLHVDTESQYAQTDTPVSFQYGA
                     SHGQAGGMTYDHKTGMLNFSSKVKATIYDTKDM"
     gene            complement(8214..8750)
                     /locus_tag="ACFM8S_001229"
     CDS             complement(8214..8750)
                     /locus_tag="ACFM8S_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KdsC family phosphatase"
                     /protein_id="HFC6314302.1"
                     /translation="MQAISPELQARAAKIKLLILDVDGVLTDGRIFIRDNGEEIKSFH
                     TLDGHGLKMLQASGVQTAIITGRDAPSVGIRVKQLGINYYFKGISDKRAAYEELRAQV
                     GVEEAECAFVGDDVVDLPVMVRCGLPVAVPDAHWFTLQHAAYIAKRPGGAGAVREVCD
                     LIMQAQGTLGAALNEYIK"
BASE COUNT         2091 a         2354 c         2240 g         2127 t
ORIGIN      
        1 tagaaattag ttaaagaata tattatggta gatactaact ctttaagttg acatattgtg
       61 cgatatcgag ggctgtaccg tattttccca tcgtattttc ccgttcttta tatgagcggc
      121 aggcatcctc ataaaagaag ggataccgat aaggaaaaat accccagcat aaaaaatata
      181 ctgattaatc aatacaaatc caacaaacag ttcatcgtat tcttttctgt tggcgtataa
      241 aaaatactga tatatgcctt cactttcata gggcgacgct ttatataccg tcctgtgtgt
      301 tgaaacgtcg gttttcctta ccgcctgccc cgcttttccc tgattgctgc caactgccgt
      361 ccgaacctga accgtcaggt ttcagacggc attttttccg cctgttccca caatttccgt
      421 ttttgtgcgg cggtcagttt tttgacggcg atttcctgtt tgagcgcagt gcctttatcc
      481 atcccgcctg caacgatacg catcgccacc ggtttgaata cgcgggtata tttcgcgcct
      541 ttaccggttg tgtgggcggc aagccgctgt tgcggattcg ggctgatgcc gcaatagaac
      601 gcgctgtttt cacacaatat caggtaaacg ctccagtttg acgcgttcat ctgcctgcat
      661 cctgagtttt ctccccgttc caaggcgcaa ctttcagcag cagcagcata cccggtaggg
      721 cgagtgcgaa acacagttgg aaaaacggta cataaccgag ccattcaatc agataaccgg
      781 caaaggaatt gatgaccgtg cgcgggacgg cggacaggct ggtaaacagc gcaagctgcg
      841 ttgcggtaaa tgcgggattg gtctcgcgcg ccatatacga tacgaacgcc gccgtcccca
      901 accccacgcc gaccgcttcc gcgccgataa ctgccgccag catcagcctc tcgcctgtgc
      961 cgaccgtgtc gaaatgtccg aaccctgcca gccatacaaa ccccaaaacg gttacagcct
     1021 gcaccgcgcc gaatagccac aaggctttgt ttacgccgat tttcagcatc cacacgccgc
     1081 ccaagatacc tgccgccact gccggccaca gtcctgcatt tttcgcaatc aaaccgatgt
     1141 ctgtcttgct gaaacccata tccagataaa acggcgttgc caacgcggtt gccatactgt
     1201 cgccgagttt gtaaaggaag ataaacagca gcacgcacac cgccgaagcg atgcccttgc
     1261 gcgtaaaaaa ttctttaaac ggctctacca cggtctgctt caacgtttta ggaacggcag
     1321 gaggcaacac gggttcgctc gcaagaaaca gcgtcatcag aaggccgggc agcataaata
     1381 atgaagtgat aacaaatact tctgaccacg gcatcctgtc tgccaacacc aaactcaatg
     1441 aaccgggaat cagggcggca atccggtagg cgttcacatg aaccgagttg cccaaaccca
     1501 attcttcgtc tgacaaaatc tcgcgcctga acgcatccaa tacaatatcc tgactggcgg
     1561 aaaaaaaagc gacaagcacc gacaagccgg caatcagcgg cagatgatta cgggggttta
     1621 aaaaggcata gaccgccaaa gccgccagca accctgcctg cgtcagcagc atccacccgc
     1681 gccgccgtcc caaaacgggc agcctgaccg cgtccatcag cggcgaccac aaaaatttcc
     1741 aagtaaacgg cagaccgatt aacgccatca gcccgatgct cttcaaatcc acctgctcgc
     1801 tgcgcaacca cgccggaatc aggttaatca gaaagtacag cggcagcccc gaggtaaacc
     1861 ccgtaaaaat acagacaagc atatttcggg aaaagatttg cccgataaaa cctgatttcg
     1921 atgcagtcat cgttcgtgca gcccaaaaaa aacaaaagcc tattgtagca aatatcgccc
     1981 gcctgctgaa aaatgccgcc ccgcgccgcc ggtttgccgt tcaaatgcaa aatgccgtct
     2041 gaaactgtgt tcagacggca ttgcttttct gaaaacccga cgtgcttaca ggctgtaata
     2101 catttcaaat tccagcggat gaggtgccat gcggatgcgg cgtacgtctt cttctttgaa
     2161 agcgatgtag ctgtcgatcc agtctttgct gaacacgccg ccgcgcagaa ggaattcgtg
     2221 gtcggctttg agggcggcga gggcttcttc caaagaggcg cagacggtcg gcaccaatgc
     2281 atcttcttcc ggcggcagat cgtacaggtt tttatcggca gggtcgcccg gatgaatttt
     2341 gttttgaata ccgtccaaac cggccatcaa caaagcggcg aacgccaagt atgggttggc
     2401 ggtcggatcg gggaaacgcg cttcgatgcg gcgtgctttg ctgctgttca cggacggaat
     2461 gcggatggaa gcggaacggt ttttggcgga ataagccagt ttggtcggcg cttcaaagtg
     2521 cggcacaagg cgtttgtagg agttggtgga cggattggta atcgcgttca gggctttggc
     2581 gtgtttgatg atgccgccga tgtaataaag ggcggtgtcg ctcaaaccgg catagccgtc
     2641 gccggcgaac aggttttgac cgtctttcca gatggattgg tgaacgtgca taccgctgcc
     2701 gttgtcgccc ataatcggtt tgggcatgaa ggtggcggtt ttgccgaagt tgtgggcaac
     2761 gttttgaatc acatatttca tgtcttgggt ttggtcggcg cgtttaacca aagtggcgaa
     2821 gcgcgtgccg atttccattt ggctgccggt accgacttcg ctgtggtgga cttcgacttt
     2881 gatgccgagt tcttccaaaa tgttcaccat ggcggagcgc aaatcttgac cgcagtcaat
     2941 cggggcaacg ggcgcgtaac cgcctttgac ggtcggacgg tggccagtat tttgaccgtc
     3001 caaatgcaag ccgcttgacc atgcgccgct ttcggaagtg atttcgtaac gggttttgtg
     3061 catgtcggtt tcaaattcta cgccgtcaaa aacgaagaat tcaggctcgg ggccgaaata
     3121 ggcggtgtcg ccgatgccgg aagatttcaa ataggcttcg gcgcggcggg cgatggagcg
     3181 cgggtcgcgg tcgtaaccct gaccgtcggc agggtcgatg acgtcgcagg taatgacgac
     3241 ggtaacatca tcataaaaag gatcgacgaa ggctgtagac gcatcgggac gcagctgcat
     3301 atcggaagcc tgaatgcctt tccagccgcc gatagacgaa ccgtcaaacg cctgaccgtt
     3361 ttcgaaccac tcttcggggt cttccaacac gatgcgcgca ggcacggtaa agtggtgctg
     3421 cttgcctttg gtatcggtaa agcgcaaatc gacaaagcgg gcttcgcttt cttcaatcaa
     3481 ttttacggcg tttttaatag acatttttca gctcctggaa aagggaaata gcaaaatatg
     3541 cggaacgcgg cggcttgacg caaagtcggc atgattctac cacgtccatg ccgtctgaaa
     3601 ctcgccctgc gtcaaatttc aagctggaaa atatagtttc agacggcaag attgtcaaca
     3661 atatgcttct gtttgtctgt tcaaaagcgg aatacccgcc cttttgtccg aatgccgaca
     3721 aaacagatgc cgtctgaaag cagttcagac ggcatctgtt gtttggtttg aaaacggtca
     3781 gcgaggatag ccgttgcaga caaatttgta ctgagcctct ccaagcgtac cgggaataac
     3841 ataattccat tcggcatagg atggtgtata agaagcgata gagtttcccg atttggaata
     3901 ccacgttgcc gtcaatatcc taaaacgttt accactgcaa tccatttctt ccaacgtttt
     3961 agctgtatta aaaaattttc cattgccata cttttgcggc ttggcgaacc ttgtttcaaa
     4021 ccatgctctg ttgtccgtac ggctgtctgc atcaccaaac gtggtattat tgtcactgtc
     4081 aatacctacc tgcacccaat ccgccgccac cgccggtgtt gccaacccca atgcaaatac
     4141 tgccaagcac aacatctttt tcatgtccga ccctctcgtc cttgtcaaca aaataaagtt
     4201 aaattgtaaa aaaaaaacga cttttttcaa gagaaaaata ttccgtcatg ctatctgaca
     4261 accgtttcag acggcatcgt ttccatctgt acgccgccgt tataaaatag ccttcccgtc
     4321 ttttggaaca cgaccatgac cgccctcccc cgctacgccg tttttggcaa ccccgttgca
     4381 cacagtaaat cgccgcaaat ccatcagcaa tttgcccttc aggaaggcgt tgacattgaa
     4441 tacgaacgca tttgcgccga catcggcggt tttgcacagg cggtttcgac attttttgaa
     4501 acaggcggtt gcggggcaaa cgttaccgta ccgttcaaac aggaagcgtt tcatctggcg
     4561 gacgagcatt ccgaacgcgc atcggcggca ggcgcggtca atacgctgat tttgctgaaa
     4621 aacggaaaac tgcgtggcga caataccgac ggtatcggtt tggccaacga catcacgcag
     4681 gtcaaaaaca ttgccatcga gggcaaaacc attttgcttt tgggcgcagg cggcgcggtg
     4741 cgcggcgtga ttcctgtttt gaaagaacac cgtcctgccc gtatcgtcat tgccaaccgt
     4801 acccatgcca aagccgagga attggcgcag cttttcggca ttgaagccgt cccgatggcg
     4861 gatgtgaacg gcggttttga tatcatcatc aacggcacat ccggcggttt gagcggtcag
     4921 cttccggccg tcaatcctga aattttccgc gactgccgcc ttgcctacga tatggtgtac
     4981 ggcgaagcgg caaaaccgtt tttggatttt gcccggcaat cgggcgcgaa acaaaccgcc
     5041 gacggactgg gtatgctggt cggtcaggcg gcggcttcct acgccctctg gcgcggattt
     5101 acgcccaata tccgccctgt tatcgaatac atgaaagcca tgtaatatgt tccgcatcgt
     5161 caaatggctg attgccctgc ccgtcggcat ctttatcttt ttcaatgcct atgtgtacgg
     5221 caacatcatc acctaccgcg ccgtcgcgcc ccatcggact gcctttatgt cgatgcggat
     5281 gaagcagttt gaacaggaag gtcgcgatgt cgcactggat taccgctgga tgccctacaa
     5341 ccgcatctcg gtcaacctga aaaaagccct gattgcttcc gaagatgccc gtttcgccgg
     5401 gcacggcggc ttcgattggg gtggcattca aaacgccatc aggcgcaacc ggaacagcgg
     5461 caaagtgaag gcgggcggct cgaccatcag ccagcagctt gccaaaaacc tgtttttaaa
     5521 cgaaagccgc agctatatcc gcaaaggcga agaagcggcg attaccgcga tgatggaagc
     5581 cgttaccgac aaagacagga tttttgaact gtatttaaac tcaatcgaat ggcactacgg
     5641 cgttttcggc gcggaagccg cgtcccggta tttttatcaa atacccgccg ccaagctgag
     5701 caaacagcag gcggcaaaac tgacggcgcg cgtccccgcc ccgctctact acgccgacca
     5761 tccgaaaagc aaacggctcc gcaacaaaac caatatcgtg ctcagacgca tgggttcggc
     5821 agagttgcct gaaagcgaca cggactgagt gccgccgcaa tataaaatgc cgtctgaaat
     5881 atcattcaga cggcatatgc tttctgcctt taccctcttc tccaaaacgc aacggggcgg
     5941 tcattatcat ttttgccctt ctacataacg gtcagacaca aaacgtgtcc gacacggaca
     6001 acttcccaaa aacggcaggc aaaaaaaata accgcttcaa ggcggttttt tggtggccag
     6061 aggcggaatc gaaccgccga cacacggatt ttcaatccgt tgctctacca actgagctat
     6121 ctggcctttg tctgtctcgt taagagatgc gtattaaacc aaattacggg acgacatgca
     6181 agcatatcgg gcaatattct ttgaaaacag gtttaagtca atgttttaaa tttgaattaa
     6241 tttatgtaaa aacagcataa atccgtattt ttgccgcctg cccgacgata ttaggtcgcc
     6301 cgtctgaaaa atattttcaa tatttgaagt tcttacccag ataaacagaa cgaacctgtt
     6361 cgtttccgac caaatcatca ggttttcccg atgccagcac cgcaccgtct gaaatgatgt
     6421 aggcgcggtc gcagatgctg agggtttcgc gtacgttgtg gtcggtaatc agtacgccga
     6481 taccgcgcga cttgagaaat ccgatgattt tttggatgtc gatgacggcg atgggatcaa
     6541 caccggcaaa aggctcatcc agcaaaataa aatgcggttt catgacgagt acgcgggcga
     6601 tttcgacgcg ccgccgttcg ccgccggaca gcgacggcgc ggggctgcgg cgcaagtgtc
     6661 cgatattaag gtcggcgagc agtttttcga tttccctgtc gatttgattt ttatctttgg
     6721 ttctgatttc caagatggcg cggatgtttt gttcgacggt cattttgcgg aatatcgagg
     6781 cttcctgcgg caggtagccg acaccgaggc gggcgcgttc gtgtatgggc aggtggcgca
     6841 attcttgtcc gtctagggtt acgctgcctg cgtcggcggc gatgagtccg acaatcatgt
     6901 agaagctggt ggttttaccc gcaccgttgg gcccgagcag accgacgact tctccgcttt
     6961 cgatttcgag ggagaagctt ttaacgactt ggcgtttttt gaaacttttt tgcaggtttt
     7021 gaacaacaag gcggctgacg tttgcgctca taaatatctc ttcaatatct tcggtcggca
     7081 aatgccgtcc gaaccaggtt tatgtttcag acggcatttt gggattattc ggatttttgc
     7141 gtactcgaag gctggataac gacgctgacc ctgccggatt tggaagcgga ttttgcgccg
     7201 gattttgtgc tgccgctgat ggtatagact tcggttttgg tgttgtatgt aatcaccgca
     7261 ccttcggcga catcgccgcc gcgctgtact ttggcattac cggttaagac tacggtgctg
     7321 cctgcagatg aataagcaac gttgtttgcc tgtccgcgca ccgtgccttt gccgccgtcc
     7381 aatgtctggc tgaagcggac tggcgaacct tccgccctca cggattcgcc gcctttgccg
     7441 ccgcgtgtaa cattgacgcg ggcggcggaa atattgagcg taccctgtct gatgacgacg
     7501 tttccgctga atgtggtgct ttggttggct tgatcgagcg aaccttggtc ggcctcaatc
     7561 tgaataggct gcctgctgtc gctttgaagg gcaaaagcgg gggacgccga aaaaaatgct
     7621 attaaaacaa atagcttaca tatcttttgt atcataaatc gtggctttca ctttagatga
     7681 gaagttcaac atgcctgttt tgtggtcgta agtcatgccg cccgcctgac cgtgcgatgc
     7741 accatattgg aaactgacag gcgtatcggt ttgggcatat tgagattcgg tatcgacgtg
     7801 cagcttttcg gcttcaactt tacccgcctg ccgtttgccg tcggcggttt tggtcagcac
     7861 aacgttgttt ttaaaaagaa cctgtttgtt ttcggtatgg taaacggctt catcgctgcc
     7921 gacttcgtac aacaacctgc cttcttggaa gaacacgaga tgcggtgagt cgaaatgaat
     7981 gtcgctgctt tcgggaaact gtttcgcgcc cttcgaactc aaatgttctt tcaagtatcc
     8041 ctgttcgtca aaccgcctgc cgtccaagcc gtccattgtg tattgcggtt cgtcgggatt
     8101 gagcctgact tcctcgattt cgacttcgct gatgcgtccc aaccatgccg acaggctgcc
     8161 caaggcaacc gccaatatca atgggaacgc aattccgtac cgccatctta ctttcatttg
     8221 atgtactcgt tcaaagccgc gcccaaagtc ccttgcgcct gcataatcag gtcgcacact
     8281 tcgcgcaccg cgcccgcacc gccggggcgt ttggcaatat aggcggcgtg ttgcagcgta
     8341 aaccaatgcg cgtcggggac ggcaaccggc aatccgcagc gtaccataac gggcaaatcg
     8401 accacgtcgt cgccgacaaa ggcacactcg gcttcttcca cgccgacctg agcgcgcagc
     8461 tcttcatacg cggcgcgttt gtccgaaatg cctttgaagt agtaatttat gcccaactgt
     8521 ttgacgcgga tgccgacgga gggcgcgtcc cgacctgtga taatcgcagt ctgcacgccg
     8581 cttgcctgaa gcattttcag accgtgtccg tccagtgtgt gaaacgattt gatttcttcg
     8641 ccgttatcgc ggataaagat gcgcccgtcg gtcaaaacac cgtccacatc caggatcagc
     8701 agtttgattt tggcggcgcg cgcctgtaat tcgggagaaa ttgcctgcat gggatttcct
     8761 tgttttcaga cggcatatct taccacaaat accgtctgaa acggctgcaa cg
//
LOCUS       DAXHSF010000075         8745 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.075, whole genome shotgun sequence.
ACCESSION   DAXHSF010000075 DAXHSF010000000
VERSION     DAXHSF010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8745
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.075"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..571
                     /locus_tag="ACFM8S_001230"
     CDS             <1..571
                     /locus_tag="ACFM8S_001230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC6314303.1"
                     /translation="FHFRKTKKECAVLGTLSRLDTVRAVHLMLDILKKMVDRGMPVRL
                     NVAGIGEEMDNLKAQAKRLGIEDKVTFLGGVRDLTGYFKEVDILVNTPHCVGDHGAGV
                     GNNILEAGLYDTPVVTYNMAGISEMVITGQTGYCIPFGDDEAFIEAVDTLIKHPELRS
                     QMGKALHKHVETLCSDDEIYRTTMAAYEM"
     gene            585..1757
                     /locus_tag="ACFM8S_001231"
     CDS             585..1757
                     /locus_tag="ACFM8S_001231"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC6314304.1"
                     /translation="MNITIVAPYCSLPSEPHFNRFWYLAELLSQSHDVLLITSNFKHY
                     DKSFRRPEDAEAASQGRLKVMLLEESGYSKNVSLGRVTSHHRFVKHFEKWLENCRPGE
                     QDVVFSAYPLIATNLLLGKHKARLGYKLIVDVQDVWPESFSSVVPFLKKVPHKLLPFA
                     SRANRAYRYADALVAVSQTYLDRAKETNPNVPGEVIYIGADFPKLDAAPAKDFGDDKT
                     RFFYLGTLSYSYDVETVCKGVRKLLDDGENVELHIMGGGPDLDRLKQYACDGIKFYGY
                     IPYAEMMSVAKGCDIAINAIQSYSMASITNKLSDYMALQKPILNSQVNDEVAEVLTLL
                     PHANYRSGDVDGFVQAAKDILKRKSDPVQSDEIVRRFKRDVAYQKIVNLIERLAHE"
     gene            1750..2991
                     /locus_tag="ACFM8S_001232"
     CDS             1750..2991
                     /locus_tag="ACFM8S_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NeuD/PglB/VioB family sugar acetyltransferase"
                     /protein_id="HFC6314305.1"
                     /translation="MNKFFKRLFDIIASASGLIVLSPVFLILAYLIRKNLGSPVFFIQ
                     ERPGKDGKPFKMVKFRSMRDALDSDGIPLPDSERLTPFGKKLRAASLDELPELWNVLK
                     GDMSLVGPRPLLMQYLPLYDNFQNRRHEVKPGITGWAQVNGRNAASWDERFACDIWYI
                     DHFSLCLDIKILLLTVKKVLIKEGISAQGEATMPPFTGKRKLAVVGAGGHGKVVADLA
                     AALGTYGEIVFLDDRVQGSVNGFPVIGTTLLLENSLSPEQFDIAVAVGNNRIRRQIAE
                     KAAALGFALPVLIHPDSTVSPSATVGQGGVVMAKAVVQADSVLKDGVIVNTAATVDHD
                     CLLDAFVHISPGAHLSGNTRIGEESWIGTGACSRQQIRIGSRATIGAGAVVVRDVSDG
                     MTVAGNPAKPLAGKNTETLRS"
     gene            3069..4244
                     /locus_tag="ACFM8S_001233"
     CDS             3069..4244
                     /locus_tag="ACFM8S_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFC6314306.1"
                     /translation="MLNTFLSPWPCFTQEEADAVSKVLLSNKVNYWTGNECRKFEKEF
                     AAFAGTRYAVALSNGTLALDVALKAIGIGAGDDVIVTSRTFLASASCIVNAGANPVFA
                     DVDLNSQNISAETIKAALTSNTKAIIVVHLAGMPAEMDGIMDLAKEHDLWVIEDCAQA
                     HGAKYKGKSVGSIGHVGAWSFCQDKIMTTGGEGGMVTTNDKTLWSKMWSYKDHGKSYD
                     AVYHREHAPGFRWLHESFGTNWRMMEMQAAIGRIQLKRLPEWTACRQAHAAKLAESLG
                     KFKSIRLVEVADYIGHAQYKFYAFVKPEHLKDGWTRDRIVNELNARKVPCYQGSCSEV
                     YLEKAFDNTPWRPKERLKNAVELGDTALMFLVHPTLTDGEIAFCKKHIEAVLAEATR"
     gene            4292..6202
                     /gene="pglD"
                     /locus_tag="ACFM8S_001234"
     CDS             4292..6202
                     /gene="pglD"
                     /locus_tag="ACFM8S_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent dehydratase PglD"
                     /protein_id="HFC6314307.1"
                     /translation="MTLETLLALPRNIKKICFLIHDFLMIFIAFWFTQSLKADYSNEW
                     FDPANWQSFLLTAPLTIGLFVRMGLYRAVTRFISFRILSTALAGSLASAVLFFLNTLI
                     FEERLRLALPIVYFLLLFVSVTGSRMVLRGLLSEHPKKQMIPVIIYGAGRSGRQLLEA
                     VKQMREYSAAAFVDDDPKLWHTVIYDLAVYQPDAIAFLIERYGVEKILLAIPGATQEQ
                     RRRIINKLEAYPCEVLTIPGMKDLMDGKISIGTLKKISVSDLLGRDSVAPDNRLMSAD
                     IEGKTVMVTGAGGSIGSELCRQIIRRRPEKLLLFELSEFALYAVEKELRETCIQKRLD
                     TEILPFLGSVQNRTLLEHVMTAFSVATVYHAAAYKHVPMVEFNTVEGIRNNIFGTLEC
                     ALAATTSGVETFVLISTDKAVRPTNTMGASKRMAELCLQALAAEPGQKTRFSMVRFGN
                     VLGSSGSVVPLFEKQIAEGGPLTLTHPEITRYFMIIPEAAQLVIQAGAMGTGGDVFVL
                     DMGESVKIIDLARQMITLSGLKPNTPEQPDGDIEILITGLRPGEKLYEELLIGDNVRK
                     TGHPRIMTANETMLPWHELSALLDRIRAACDRYDQQAIRTLLINAPTGFAPSDGICDL
                     LWVRETHRKNAV"
     gene            6258..7550
                     /locus_tag="ACFM8S_001235"
     CDS             6258..7550
                     /locus_tag="ACFM8S_001235"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFC6314308.1"
                     /translation="MQFSAFGEKFTQHSGILQLMDDLGDALKSDKPVNMLGGGNPARI
                     AEVNAVFADVFSKLAAEHAVENIGNYSNPQGDAALIDALTAFLNREYGWNLTADNIAL
                     TNGSQNAFFYLFNLFGGKFKLSDGTSAEKAILLPLAPEYIGYADVHIEGQHFVSVKPK
                     IENVEHEGEAGFFKYRVDFDALENLPELKAGKIGAICCSRPTNPTGNVLTDGEMARLD
                     ALAREHGIPLIIDNAYGMPFPNIIYSDVTLNWHENIILCFSLSKVGLPGVRTGIIVAA
                     PEVVKAVSSLNAIVNLAPTRFGAAIAVPLLESGEMKRLADQVIRPFYHNQAQTAVSLL
                     KRELGAYPMKIHKPEGAIFLWLWFENLPVSSQTLYEMLKAEGTLIIPGEHFFVGIDTR
                     DYPHAGECIRMSIAQDAQTLEKGIAAIGKTVRKLYDNV"
     gene            complement(7602..8438)
                     /locus_tag="ACFM8S_001236"
     CDS             complement(7602..8438)
                     /locus_tag="ACFM8S_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFC6314309.1"
                     /translation="MIDLHCHSTVSDGMLSPAEVVRLAHQNGCTLLALTDHDHTGGIA
                     EARAEADRLGLRLINGVEISVTWRGRTIHVVGLDFDEQDENLQNLLAQVRQGRLKRLE
                     AIAAKLEKRGISGAYDGALALAANKEMVSRTHVAEFLIRVGHVKNKQQAFTKYLGDGK
                     SCAVRHEWATLEDCVSAVNGAGGLAVIAHPMRYDLSATAKRNLFEEFKNLGGAGIEVH
                     SGNCCKNDRLNYALLAERFGMLASAGSDFHRLNDFSGGILGACPELPENCRPVWAHFS
                     RY"
BASE COUNT         2094 a         2651 c         2210 g         1790 t
ORIGIN      
        1 atttcatttc cggaaaacca aaaaagaatg cgccgtactc ggcacacttt cccgtttgga
       61 caccgtccgc gccgtgcatc tgatgttgga tattttgaag aaaatggttg accgcggtat
      121 gcctgtacgc ctcaacgttg caggtatcgg cgaagaaatg gacaacctca aagcccaagc
      181 caaacgtttg ggcattgaag acaaagtcac cttcctcggc ggcgtccgcg atttgaccgg
      241 ctacttcaaa gaagtcgata ttttggtgaa cacgccgcat tgcgtgggcg atcacggcgc
      301 aggtgtcggc aacaatattt tggaagccgg cctttacgac acgcctgtcg tgacctacaa
      361 catggcgggc atttccgaaa tggtcattac cggccaaacc ggctactgca ttcctttcgg
      421 cgacgacgaa gcatttatcg aagccgtcga tacactcatc aagcatccag agttgcgcag
      481 ccaaatgggc aaagccctgc acaaacatgt cgaaacctta tgctccgacg acgaaatcta
      541 ccgcaccacc atggctgcgt acgaaatgta aggccgtctg aaacatgaac attaccatcg
      601 tcgcccctta ttgctcgctg ccgtccgagc cgcatttcaa ccgcttctgg tatcttgccg
      661 agctgttgtc gcagtcgcat gacgtgttgc tgattaccag caacttcaag cactacgaca
      721 aatctttcag acggcccgaa gatgccgagg ccgcctcaca aggccgtctg aaagtcatgc
      781 tgttggaaga aagcggatac agcaaaaacg tgtctttagg acgcgtgacc agccatcacc
      841 gcttcgtcaa acattttgaa aaatggttgg aaaactgccg tccgggcgaa caagacgtcg
      901 tcttttccgc ctatccgctg attgccacca acctgctgtt gggcaaacac aaagcgcgtt
      961 tgggttacaa actgattgtc gatgtgcagg acgtatggcc ggagtctttc tcctcggtcg
     1021 taccgttttt gaaaaaagta ccgcacaagc tgctgccctt tgcttcacgc gccaaccggg
     1081 cctaccgcta cgccgacgcg ctggttgccg tatcgcagac ttatctcgac cgcgccaaag
     1141 aaaccaatcc gaacgtaccc ggcgaagtca tctatattgg tgctgacttt cccaaactcg
     1201 atgccgcacc tgccaaagat tttggcgacg acaaaacccg ctttttctac ttgggcacgc
     1261 tcagttacag ctatgacgtg gaaaccgtgt gcaaaggcgt tcggaaactt ttggacgacg
     1321 gcgaaaatgt agagctgcac atcatgggcg gcggccccga tttggacagg ctcaaacaat
     1381 atgcctgcga cggcatcaag ttttacggct acattcctta cgccgaaatg atgtcggtcg
     1441 ccaagggctg cgacattgcc atcaacgcca tccagtccta ttcaatggca tcgattacca
     1501 acaaactctc cgactatatg gctttgcaaa aaccgattct gaacagccag gtcaacgacg
     1561 aagttgccga agtccttacc ctgctaccgc atgcgaacta ccgttccggt gatgtggacg
     1621 gtttcgttca agccgccaaa gatattttga agcgcaaaag cgatcctgtt cagtccgacg
     1681 aaatcgtccg ccgcttcaaa cgcgatgtgg cgtatcagaa aatcgtcaac ctgattgaaa
     1741 gattagctca tgaataaatt tttcaaacgc ctgttcgaca tcatcgcctc cgcatcgggg
     1801 ctgattgtcc tctcacccgt tttcctgatt ttggcgtacc tcatccgcaa gaatctgggt
     1861 tcgcccgtct tcttcattca agaacgcccc ggaaaggacg gaaaaccttt taaaatggtc
     1921 aaattccgtt ccatgcgcga cgcgcttgat tcagacggca ttccgctgcc cgacagcgag
     1981 cggctcacac ccttcggcaa aaaactgcgt gccgctagtt tggacgaact gcctgaatta
     2041 tggaatgtct taaaaggcga catgagcctg gtcggcccgc gcccgctgct gatgcaatat
     2101 ctgccgctgt acgacaactt ccaaaaccgc cgccacgaag tgaaacccgg cattaccggc
     2161 tgggcgcagg tcaacgggcg caacgccgcc tcatgggacg aacgcttcgc atgcgacatc
     2221 tggtatatcg accacttcag cctgtgcctc gacatcaaaa tcctactgct gacggttaaa
     2281 aaagtattaa tcaaagaagg gatttccgca cagggcgaag ccaccatgcc ccctttcaca
     2341 ggaaaacgca aacttgccgt cgtcggcgcg ggcggacacg gaaaagtcgt tgccgacctt
     2401 gccgccgcac tcggcacata cggcgaaatc gtttttctgg acgaccgcgt ccaaggcagc
     2461 gtcaacggct tccccgtcat cggcacgacg ctgctgcttg aaaacagttt atcgcccgaa
     2521 caattcgaca tcgccgtcgc cgtcggcaac aaccgcatcc gccgccaaat cgccgaaaaa
     2581 gccgccgcgc tcggcttcgc cctgcccgtc ctgattcatc cggactcgac cgtctcgcct
     2641 tctgcaacag tcggacaagg cggcgtcgtt atggcgaaag ccgtcgtaca ggctgacagc
     2701 gtattgaaag acggcgtgat tgtgaacact gccgccaccg tcgatcacga ttgcctgctt
     2761 gatgctttcg tccacatcag cccgggcgcg cacctgtcgg gcaacacgcg tatcggcgaa
     2821 gaaagctgga taggcacagg cgcgtgcagc cgccagcaga tccgtatcgg cagccgcgca
     2881 accattggag cgggcgcagt cgtcgtgcgc gacgtttcag acggcatgac cgtcgcgggc
     2941 aacccggcaa aaccattggc aggcaaaaat accgagaccc tgcggtcgta aacgattaaa
     3001 ataaacagcc taagcaacag acggcagaaa tgaggaaatg ccgtctgaaa accgacggac
     3061 caaacaccat gctgaacact ttcctttccc cgtggccctg cttcacccaa gaagaagccg
     3121 atgccgtttc caaagtcctg ctgtccaaca aagtcaacta ctggacggga aacgaatgcc
     3181 gcaaatttga aaaagaattc gccgcctttg ccggcacgcg gtacgccgtc gccctttcca
     3241 acggcacgct ggcactcgat gtcgcgctca aagcaatcgg cataggcgcg ggcgacgatg
     3301 tgattgttac ttcgcgcact ttcttggctt ccgcttcctg cattgtgaac gcgggcgcaa
     3361 atcccgtgtt tgccgatgtg gatttgaaca gccaaaacat cagcgccgaa actatcaaag
     3421 ccgcgctgac gtcgaatacc aaagccatca tcgtcgtcca cctcgccggt atgcccgccg
     3481 aaatggacgg catcatggac ttggcaaaag aacatgattt gtgggtcatc gaagactgcg
     3541 cccaagcgca cggcgcgaaa tacaaaggca aatccgtcgg cagcatcgga cacgtcggcg
     3601 cgtggtcgtt ctgccaagac aaaatcatga ccacgggcgg cgaaggcggc atggttacca
     3661 ccaacgacaa aaccctgtgg agcaaaatgt ggtcgtacaa agaccacggc aaaagctatg
     3721 acgccgtgta ccaccgcgaa cacgcccccg gtttccgctg gctgcacgaa agtttcggca
     3781 caaactggcg tatgatggaa atgcaggcgg caatcgggcg catccagctc aaacgcctgc
     3841 ccgaatggac tgcctgccgt caagcgcacg ccgccaagct ggcggaaagc ctaggcaaat
     3901 tcaaaagtat ccgcttggtt gaagtcgccg actacatcgg acacgcgcaa tataagttct
     3961 acgccttcgt caaacccgaa cacctcaaag acggctggac gcgcgaccgc atcgtgaacg
     4021 aactgaacgc acgcaaagtc ccctgctatc aaggtagctg ctccgaagtc tatttggaaa
     4081 aagccttcga caacacgccg tggcgaccga aagagcgttt gaaaaacgcc gtcgaactgg
     4141 gcgacaccgc cctgatgttt ttggtgcacc cgacgctgac cgacggcgaa atcgcgttct
     4201 gcaaaaaaca catcgaagcc gtattggcag aagccacacg ataacccttc agacggcata
     4261 tgccgcctga aagcacatac cgccccacga tatgactctg gaaaccctgc ttgccctacc
     4321 gcgcaacatc aagaaaatct gtttcctcat acacgatttt ctgatgattt tcattgcctt
     4381 ttggtttacc caaagcctga aggccgacta ctcgaacgaa tggttcgacc ctgccaactg
     4441 gcagtctttc ctattgaccg cgccgttgac catcggcctg ttcgtcagaa tggggctcta
     4501 ccgtgccgtt acacgcttca tcagcttccg catcctctcc accgcgctgg caggcagcct
     4561 cgcctccgcc gtattgttct tcctcaacac attgattttt gaagaaaggc tgcgcctcgc
     4621 cctgccgatt gtctatttct tactgctgtt tgtttccgtg accggctcgc gtatggtttt
     4681 gcgcggactg ttgtccgaac accccaaaaa acagatgatc cctgtcatca tttacggcgc
     4741 gggacggtcg ggcagacaac tgcttgaggc cgtcaaacaa atgcgcgaat attccgccgc
     4801 cgcctttgtg gacgacgacc ccaaactgtg gcacaccgtc atctacgacc ttgccgttta
     4861 ccagcccgat gccatcgcct tcctcatcga acgctacggc gtggaaaaaa tcctactcgc
     4921 catccccggc gcgacccagg aacaacgccg ccgaatcatc aacaaactgg aagcctatcc
     4981 gtgcgaagtg ttgaccattc ccggaatgaa agacctgatg gacgggaaaa tcagcatcgg
     5041 cacgctcaaa aaaatctctg tgtccgacct gctcgggcgt gattccgtcg cgcccgacaa
     5101 ccgcctgatg agtgccgaca tcgaaggcaa aaccgtcatg gtaaccggcg cgggcggctc
     5161 catcggttcg gaactctgcc gccagattat ccgccgccgc cccgaaaagc tgctgctgtt
     5221 cgagttatcc gaattcgccc tgtacgccgt cgaaaaagaa ttgcgcgaaa cctgcatcca
     5281 aaaacgcctc gacaccgaaa tcctgccctt tctcggttcg gtgcaaaacc gcacgctgct
     5341 cgaacacgtc atgaccgcct tttccgttgc gaccgtctat cacgccgccg cctacaaaca
     5401 cgtccccatg gtcgagttca acaccgtcga aggcatacgc aacaacatct tcggcacact
     5461 cgagtgcgcg cttgccgcca cgacatcggg cgtggaaact ttcgtcctca tctccaccga
     5521 caaagccgtc cgccccacca acaccatggg tgccagcaaa cgcatggcgg aactctgcct
     5581 tcaggcactc gccgccgaac ccggacaaaa aacccgcttc agcatggtac gtttcggcaa
     5641 tgttttaggt tcgtccggct ccgttgtccc gctgtttgaa aaacagattg cagaaggcgg
     5701 cccgcttacc ctgacccacc ccgaaatcac acgttatttc atgatcatac ccgaagccgc
     5761 ccaactcgtc atacaggcag gcgcgatggg tacgggcggc gacgtattcg tcctcgacat
     5821 gggtgaatcc gtcaaaatca tcgaccttgc ccgccaaatg attaccctaa gcggcctcaa
     5881 acccaacaca cccgaacaac ccgacggcga catcgaaatc ctcattaccg gactgcgtcc
     5941 cggagaaaaa ctctacgaag agctgctcat cggcgacaac gtccgcaaaa ccggccatcc
     6001 gcgcatcatg accgccaacg agaccatgct gccgtggcac gagctctccg ccctgctcga
     6061 ccgcatccgt gcggcctgcg accgttacga ccagcaggca atccgcaccc tgctcatcaa
     6121 cgccccgacc ggctttgccc cgagcgacgg catctgcgac ctgctttggg tacgagaaac
     6181 acacagaaaa aatgccgtct gaaccttcag acggcataac gtacaaacca acctacctta
     6241 cacacacgga gtttgacatg cagttttcag cattcggcga aaaattcacg caacacagcg
     6301 gcatcctcca actgatggac gacctcggcg acgcgctcaa aagcgacaag cccgtcaata
     6361 tgctcggcgg cggcaatccg gcgcgaatcg ccgaagtcaa tgccgtgttc gccgacgtat
     6421 tttccaaact ggcagcagaa catgccgtcg aaaacatcgg caactactcc aatcctcaag
     6481 gagatgccgc gctgattgac gcgctgaccg ccttcctcaa ccgcgaatac ggctggaacc
     6541 tgaccgccga caatatcgcg ctgaccaacg gttcgcaaaa cgcgtttttc tatcttttca
     6601 acctcttcgg cggcaaattc aagctttcag acggcacatc cgcagaaaaa gccattttgt
     6661 tgccgctcgc gcccgaatac atcggctatg ccgacgtaca tatcgaaggg cagcacttcg
     6721 tttccgtcaa gcccaaaatc gaaaacgtcg aacacgaagg cgaagcaggc ttcttcaaat
     6781 accgcgtgga ctttgacgca ctggaaaacc tgcccgaact caaagcgggc aaaatcggcg
     6841 cgatttgctg ttcgcgcccg accaacccga ccggcaatgt gttgaccgac ggcgaaatgg
     6901 cgcgtttgga cgctttggcg cgtgaacacg gcattccgct gattatcgac aacgcctacg
     6961 gaatgccgtt ccccaacatt atttacagcg atgtaacgct gaattggcac gaaaacatca
     7021 tcctctgctt cagcctttcc aaagtcggcc tgccgggcgt tcgcaccggc atcatcgtcg
     7081 ccgcgcccga agtcgtcaaa gccgtcagca gcctgaacgc gattgtgaac cttgccccca
     7141 cgcgcttcgg cgcggcgatt gccgtcccgc tgctggaaag cggcgagatg aaacggcttg
     7201 ccgaccaagt catccggccg ttttaccaca atcaggcaca aaccgccgtc tcgctgctca
     7261 agcgcgagct gggcgcgtac ccgatgaaaa tccacaaacc cgaaggcgcg attttcctgt
     7321 ggctctggtt tgaaaacctg cccgtttctt cgcaaaccct gtacgaaatg ctcaaagccg
     7381 aaggtacgct gattattccg ggcgagcatt tcttcgtcgg catcgacacg cgggattacc
     7441 cgcacgcggg cgagtgcatc cgcatgagca tcgcgcagga cgctcaaacg ctggaaaaag
     7501 gcattgccgc catcggtaaa accgtccgaa aactgtacga caacgtttaa accgcaaaaa
     7561 atgccgtctg aaaggttttc agacggcatt tttatctgca ttcaatatcg ggaaaaatgc
     7621 gcccacaccg gcctgcagtt ttccggcagt tcgggacacg cgccgagaat gccgccgcta
     7681 aagtcgttta aacggtggaa gtcgctgccc gcgctggcga gcataccgaa gcgttctgcc
     7741 aaaagcgcgt agttgaggcg gtcgtttttg cagcagttgc cgctgtggac ttcgatgcct
     7801 gcgccgccga ggtttttaaa ttcttcaaac agattgcgct tggcggtggc ggacaaatca
     7861 taacgcatcg gatgggcgat gaccgccaac ccgcccgcgc cgttgacggc ggagacgcag
     7921 tcttccagcg tcgcccattc gtggcggacg gcgcaggatt tgccgtcgcc caagtatttg
     7981 gtgaacgcct gctgcttgtt tttgacgtgt cccacccgaa tgaggaactc ggcgacgtgt
     8041 gtgcggctga ccatttcttt gtttgccgcc agcgcgagcg cgccgtcgta tgcgcccgaa
     8101 atgccgcgtt tttccagctt ggcggcgatg gcttcgaggc gtttcagacg gccttgccgc
     8161 acttgcgcca acaggttttg caggttttcg tcttgttcgt caaagtctaa gccgacaacg
     8221 tgtatggtac gcccgcgcca cgttacggag atttccacgc cgttaatcag gcgcaaaccg
     8281 agcctgtcgg cttcggcacg cgcttcggcg atgccgccgg tgtggtcgtg gtcggtcaac
     8341 gccagcagcg tgcagccgtt ttgatgcgcg aggcgcacga cttcggcggg ggaaagcata
     8401 ccgtcggaaa cggtggaatg gcagtgcagg tctatcatgg ggtgttatat gtgtggtgaa
     8461 tgaaggtcgg gggcaatatt gtcgggcagt gtggcggcgg cggtcatttt aaaaaacagg
     8521 tgtatggcaa atgcaaagaa aaagtcccta taccgtcatt cccgcgcagg cgggaatcca
     8581 gactttgatt tattggaaat gtttaaggtt aaccgctatt tcaaacttcc ggattcccgc
     8641 ctgcgcggga atgacgatag ggatgttttc aattttaatc tactataaaa gactgtctga
     8701 aaacgtggtt ttatagtgaa ttaaatttaa accggtacag cgttg
//
LOCUS       DAXHSF010000076         8731 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.076, whole genome shotgun sequence.
ACCESSION   DAXHSF010000076 DAXHSF010000000
VERSION     DAXHSF010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8731
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.076"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(125..328)
                     /locus_tag="ACFM8S_001237"
     CDS             complement(125..328)
                     /locus_tag="ACFM8S_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFC6314310.1"
                     /translation="MELGYTPYNLRNRCKLIQAELAQIVGVKHYIQVGRWEAEPDTET
                     RRADMPLEKWRQFLDWTEKTNAV"
     gene            complement(340..621)
                     /locus_tag="ACFM8S_001238"
     CDS             complement(340..621)
                     /locus_tag="ACFM8S_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314311.1"
                     /translation="MNRAALFNRYPEWIIGQDGASRFITHCRYPRLIAKIHRQTDGEC
                     PGGHYRHSENGITLYDFIFFDGKPADEARFAAVLTETCRRAVKKICETK"
     gene            complement(659..862)
                     /locus_tag="ACFM8S_001239"
     CDS             complement(659..862)
                     /locus_tag="ACFM8S_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314312.1"
                     /translation="MNIIEQEMKHSSLRKVDVEIAKIIADAHKINAESVKIAQESRWY
                     PMMAATGLVTAIAAVLALIFKFA"
     gene            complement(942..1514)
                     /locus_tag="ACFM8S_001240"
     CDS             complement(942..1514)
                     /locus_tag="ACFM8S_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFC6314313.1"
                     /translation="MGKPGKSSRPSALLSARWQDIDLTARLWHIPAADMKTKHPFTVP
                     LSDWAVEILRELHTQTGDNIHLFPGIRPRRKPAPHPDHISIKFAHNAIRRLGYDGSTP
                     GKSKHTMHGFRHLFTNISLTAGKDTLTTDLALGHISRAALQRAGFSSLHHYLTADSYR
                     LQERRELAEWYSRRHRQAYEAAAQNTCNKT"
     gene            complement(1489..1833)
                     /locus_tag="ACFM8S_001241"
                     /pseudo
     CDS             complement(1489..1833)
                     /locus_tag="ACFM8S_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701232.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(1826..2083)
                     /locus_tag="ACFM8S_001242"
                     /pseudo
     CDS             complement(1826..2083)
                     /locus_tag="ACFM8S_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237890.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2094..2414
                     /locus_tag="ACFM8S_001243"
     CDS             2094..2414
                     /locus_tag="ACFM8S_001243"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314314.1"
                     /translation="MLGVLDCYQKSQSCPNPKSHCCFVSDDIFLWHGSRPTFEISLDL
                     TGKKKETSSAKKFVMPCINFRHHRIEKETYLGNWNEQIAALAVKQNIDWCPSFDIENF
                     RQYE"
     gene            2407..2601
                     /locus_tag="ACFM8S_001244"
     CDS             2407..2601
                     /locus_tag="ACFM8S_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6314315.1"
                     /translation="MNNYYLYRNCSSDVLWVKRIQRQIDGSLLLISDNSTYPPMPLAL
                     AEHPDIQIIGQVVQVSKDLN"
     gene            2625..3185
                     /locus_tag="ACFM8S_001245"
     CDS             2625..3185
                     /locus_tag="ACFM8S_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314316.1"
                     /translation="MKILALLIAATCALSACGGQAEEQPASAQPQEQAQSELKTMPVS
                     YTDYQSVANKGLADQKTGLTLPEHVVPADNAEGKNLLHDFSDGLTLTVDTDKADKITA
                     VRVVWNTDAVPQKAEKLSKAAAALIAATAPGDRTLLRDTGDQIKMAIDSHNAQKEPTR
                     EWARGGIAYKVTVTNLPSVVLTAKAE"
     gene            3326..4384
                     /locus_tag="ACFM8S_001246"
     CDS             3326..4384
                     /locus_tag="ACFM8S_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314317.1"
                     /translation="MKNKLTLRYFKITRCGYWSGSSKEPNHYEDLLKTLTHLETYVKG
                     KTIEETGISRDDKEPSTFLLDICKKNGLWLLAFWNPAAESSSDEIVSLNMKSKLGQVD
                     SSPVTAGNGKTFGFVSYFLIWPEHNFYSTILSPNATLAGRTEFEEYMLNFLWIDPNFI
                     EKTTYEETSTDGIIHTMERTNFKLPLKINGTPVPRFAGKLAKSPSEKEYVISNYDQVT
                     QVHNQCTVKVTSGQSSSGILKKMLPSFFGSTELFAPLKTIRVNHSVSAAFENKSNLKD
                     WIEDWEKNSFDLDKDDCGFKLKNDDKIYWIRGNVIKCELDVNLHKENGIYPAAKILDI
                     IDQRKDYVLNEFKLSDDS"
     gene            4374..4880
                     /locus_tag="ACFM8S_001247"
     CDS             4374..4880
                     /locus_tag="ACFM8S_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314318.1"
                     /translation="MILRNIFLICSAFMIGVAWYLGANIRFEEQWVLYEALRTTASII
                     FAVVGVWLAIVYPERLKSPFSQTFSSPVYRRGFKQLFSPVISSIFILSIVLLVGLLAP
                     LLKHIEAIIQYIPLCRKISFSLLASLTMWQIYTVLSVLVPLLNILSKNENDCHASNII
                     NKLKGEQN"
     gene            4984..5487
                     /locus_tag="ACFM8S_001248"
     CDS             4984..5487
                     /locus_tag="ACFM8S_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HFC6314319.1"
                     /translation="MSTKFNQFINRVLSHEGGYVNHPKDPGGETNWGITKRTAQANGY
                     NGSMRAMTREQAIGIYRKAFWERYRADQMPEAVAFQFFDACVNHGYGNAVRMLQRAAG
                     VADDGIIGEISLKAINSLPENDLLLRFNAERLVFYTKLSTFTSFGKGWVRRVAQNLIH
                     ASADNTD"
     gene            5599..5931
                     /locus_tag="ACFM8S_001249"
     CDS             5599..5931
                     /locus_tag="ACFM8S_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314320.1"
                     /translation="MLDASPPQERGSRRETRSQKKASQITAFFRWLGGLVSNPATGKI
                     SHTKLWANVAAAAMTWKFVQAADAPEWLWWAYGAMVGGYALIKRGIAVVPQLAEVKKS
                     ANQEGGRQ"
     gene            5928..6338
                     /locus_tag="ACFM8S_001250"
     CDS             5928..6338
                     /locus_tag="ACFM8S_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HFC6314321.1"
                     /translation="MIEFVRAKKRLLWAFVLLLVWTCGYRYAADKAEAEQTALIATYR
                     HSSMVAAEQYALQLKKAQDERQRWYDFSQKQGRKPVKKQYPPQTKKAGYLKTKEELLA
                     ELACLTAEMAALKKPDALIHGKEVRQKERNSSQG"
     gene            6343..6891
                     /locus_tag="ACFM8S_001251"
                     /pseudo
     CDS             6343..6891
                     /locus_tag="ACFM8S_001251"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            6907..7092
                     /locus_tag="ACFM8S_001252"
                     /pseudo
     CDS             6907..7092
                     /locus_tag="ACFM8S_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(7411..7773)
                     /gene="ssrA"
                     /locus_tag="ACFM8S_001253"
     tmRNA           complement(7411..7773)
                     /gene="ssrA"
                     /locus_tag="ACFM8S_001253"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(7836..8441)
                     /locus_tag="ACFM8S_001254"
     CDS             complement(7836..8441)
                     /locus_tag="ACFM8S_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HFC6314322.1"
                     /translation="MNTDFQNILKQLGKQAQKEAAEKQAAEKNKQKQEQDFDFSQAVG
                     QVSPLKNRQQYYAPPDKTPIKVRPKDNRADEENYFYIGSTYNDPPASFSKNGQGKNDI
                     QRLKNGYYPVVTDVDLHGYTQEEAQKVLNEFIAFTQKRGVCGEIIHGSGLGSKGYKPV
                     LKNMTRNWLMQHPDVLAYAEPREGNDGCVRILLKRKLRQQD"
     gene            complement(8485..>8731)
                     /locus_tag="ACFM8S_001255"
     CDS             complement(8485..>8731)
                     /locus_tag="ACFM8S_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314323.1"
                     /translation="SDFRVSRFRQFKIGKTRQPASQGRFLKLYQAFGHIGSTGPHFRQ
                     STLPTVAAFLFDIQSHFQKTYAGNKIILPRLANIPAN"
BASE COUNT         2195 a         1926 c         2226 g         2384 t
ORIGIN      
        1 gtgagtttat tttggggcgg cggcaggtcg ggggcaagcg gcgtggggct tggttgtggt
       61 ttttaggttt gggggtgggt aaaaaatgcc gtacccggcg ggcaggcttc gcactctgaa
      121 ggtttcagac ggcgtttgtt ttttctgtcc aatcgaggaa ctgccgccat ttttccagcg
      181 gcatatcggc ccgccgcgtt tcggtatcgg gttcggcttc ccagcggcct acctgtatgt
      241 agtgcttcac cccgacgatt tgcgccaact cggcctgtat cagtttgcag cggttgcgca
      301 ggttgtaagg tgtgtagccg agttccatgt cgttgcggtt tattttgttt cgcatatttt
      361 tttgactgcc cggcggcagg tttcggtaag gacggcggca aatcgggctt cgtctgccgg
      421 ttttccgtcg aaaaaaataa aatcgtaaag ggtgatgccg ttttcggaat ggcggtaatg
      481 cccgcccggg cattcgccgt ctgtctgcct gtggattttg gcaatcaggc ggggatagcg
      541 gcaatgggta atgaaacggc ttgcgccgtc ttgcccgata atccattcgg ggtagcggtt
      601 gaatagtgcg gctctgttca ttttgttcgg gggataaagc ctctcgcggg gcttgtggtc
      661 aggcaaattt gaatatcagt gccaacacgg cggcgatggc ggtaaccagc ccggtggccg
      721 ccatcatggg ataccatcgg gattcttggg ctatttttac ggattcggcg ttgattttgt
      781 gcgcgtctgc aatgattttg gcgatttcga catctacttt tctcagactg gagtgtttca
      841 tttcttgttc gattatgttc atcgtacttc ctttcgtttt tggcggttgc cgccgcttgt
      901 cggatggtag gatgtctgcc atgtgtatat attgatacct tttaggtttt attgcaagtg
      961 ttttgggcgg cggcttcgta tgcttggcgg tggcggcggc tgtaccattc ggcgagttcg
     1021 cgccgctctt ggaggcggta gctgtcggcg gtcaggtagt ggtgcaggct tgagaagccg
     1081 gcgcgttgga gggcggcgcg gctgatgtgg ccgagggcga ggtctgttgt gagggtgtct
     1141 tttccggcgg tcaggctgat gttggtgaag aggtggcgga atccgtgcat tgtgtgtttg
     1201 gatttgccgg gggtgctgcc gtcatagccc agtcgtcgga tggcgttgtg ggcgaatttg
     1261 atgctgatgt ggtcgggatg gggggcgggt ttgcgccgtg ggcggatgcc ggggaaaagg
     1321 tggatgttgt cgccggtctg cgtgtgcagc tctcggagta tttctaccgc ccagtccgac
     1381 agtgggacgg taaaggggtg tttggtcttc atgtcggcgg cggggatgtg ccataaccgg
     1441 gcggtgaggt cgatgtcttg ccagcgggcg gaaagcagtg cggacggacg ggatgacttc
     1501 cccggtttcc ccatcaatcc gcgccgcctc gatttttccg ttggcgcgca gcatttccac
     1561 cgcctgccgc accgacatca accgcttgcc gttgcgcctc atcgcctcca ccaaaaccgc
     1621 cgaaatcaat ttggcttctt ccggtttaag ctccgtcttg cccgcatcgc tgcgccgttt
     1681 gcgcgtcggc ttgatgctga ccgcctccag cttgcggtat agcgtggcaa ggctgatgcc
     1741 caattcctgc gcctgctgct taagatatgc agagcgtgcg ccgcgtccca ttgcttccgc
     1801 ctgattctcg actgccttaa gacgctcaat cattgccgga ttcatcgcct tctcccgttt
     1861 caccgcccaa ccattccggc acattgtctg tcggtgcttc agtcggcagg gcatagcttt
     1921 cgcgcagttg ctcgcagtcc aaaataattt gattgagcgt gccgaccatc tttgcctgat
     1981 ggctgatctc gtgtgcctca ctgtgcgcat taagttggtc gaacaaatct ttcaaacggc
     2041 tcacttgact gcggataccg acctcaaggc ttgttaactg catcatttat gatttgttag
     2101 gggttttaga ctgctatcaa aaatcacagt cctgtcccaa ccctaaaagc cactgttgct
     2161 ttgtttcaga cgacattttt ctatggcatg gatcaagacc aacgtttgaa atcagtcttg
     2221 atctaactgg aaagaaaaaa gaaacatcct ctgcaaagaa atttgtgatg ccttgtatta
     2281 atttccgtca ccataggatt gaaaaagaaa cctacttagg aaattggaat gaacaaattg
     2341 ccgcattggc agtaaaacaa aatatagatt ggtgtccaag ttttgatatt gagaatttta
     2401 gacagtatga ataattacta tctatatagg aattgcagca gcgatgtgtt atgggtcaaa
     2461 cgtatccaac gccaaatcga cggcagccta ctcttgattt ctgacaattc aacctatcca
     2521 cccatgccct tggcactggc ggaacacccc gatattcaaa tcatcgggca ggtagtgcag
     2581 gtatcaaaag acttgaacta gacacaatca aaaagggaaa tagaatgaaa atactcgctt
     2641 tattaattgc cgctacctgt gctttatctg cgtgtggcgg ccaagctgaa gaacaaccgg
     2701 catctgccca acctcaagag caggcacaat ccgaattaaa aaccatgccg gtaagctata
     2761 ccgactatca atcagtagcc aataaagggc ttgccgacca aaaaactggt ctgacccttc
     2821 cagaacatgt tgttcctgcc gacaatgcgg aagggaagaa tctgctgcat gacttttcag
     2881 acggcctcac attaaccgtt gataccgata aagccgacaa aattactgcc gtccgagtag
     2941 tttggaatac agatgcagtg cctcaaaaag cggaaaaact gtccaaagcc gccgcagcct
     3001 tgattgcggc aaccgcccca ggagaccgca cactgctgcg tgataccggc gaccaaatca
     3061 aaatggcaat tgacagccat aatgcgcaaa aagagccaac ccgagaatgg gcgcgtggtg
     3121 ggattgctta taaagtcact gttactaatt tgccgagcgt ggttttgaca gcaaaagctg
     3181 aataacctgc attacagcca caattaggag cagacggaga tgatgtttat agagcctgct
     3241 gagaataaat aactgataaa aaagctgtct gaaaaggttt cggatagtta ggatagctat
     3301 ctatttttaa tatattagag agaatatgaa aaataaactg actttacgtt atttcaaaat
     3361 aactcgttgc ggttattggt ctggttcttc aaaagaacct aatcattatg aggacttatt
     3421 aaaaacttta actcatttag agacatatgt taaaggtaaa actattgaag agactggaat
     3481 tagtcgtgat gataaagagc caagcacttt cttgctcgat atctgcaaaa aaaatggtct
     3541 gtggttgctt gctttttgga atcctgccgc agaaagctct tcagatgaaa ttgtttcttt
     3601 aaacatgaaa agtaaactag gtcaagttga ttctagccca gtaacagcag gaaatgggaa
     3661 aacttttggc ttcgttagtt attttcttat ttggccagaa cataattttt actcaacgat
     3721 tctttctcct aatgcaactt tagctggacg aacagagttt gaagagtaca tgcttaattt
     3781 cttgtggatt gatcctaatt ttatagaaaa gactacctac gaagaaactt caacagacgg
     3841 aataattcat accatggagc gaactaattt taaattgcct cttaaaatta atggaactcc
     3901 tgtgccacgt tttgcgggga aactagctaa atctccttca gaaaaagagt atgtcattag
     3961 taattatgac caagtaaccc aagtgcataa tcaatgcacg gtaaaggtta cctctggaca
     4021 aagttcaagt ggaattttga agaaaatgtt accaagcttt tttggttcaa cagaattgtt
     4081 tgctccatta aaaaccattc gtgtaaatca ttctgtttct gctgcatttg aaaacaaatc
     4141 aaatcttaag gattggattg aagactggga aaaaaattct tttgatttag ataaagatga
     4201 ttgtggcttt aagttaaaaa acgatgacaa gatttattgg attcgaggaa atgtcatcaa
     4261 atgtgaatta gacgtcaacc ttcataagga aaatgggatt tatccagctg ctaaaattct
     4321 agatataata gaccaaagga aagattacgt tttaaacgaa ttcaaattaa gtgatgattc
     4381 ttagaaatat tttcctaata tgctctgcat tcatgattgg tgtagcctgg tatttaggtg
     4441 ctaatatccg atttgaagag caatgggtat tgtatgaagc cttgagaaca actgcatcaa
     4501 ttatttttgc agttgttggg gtttggttgg ctattgtcta tcccgaacga ttaaaaagcc
     4561 cttttagtca aactttctcc tctccggtat atcggcgtgg attcaaacag cttttttccc
     4621 ctgttataag ttccattttt attttaagta ttgtgttact tgttggtttg ttggctcctt
     4681 tgctcaaaca tattgaggca ataattcaat atatcccact ttgtcggaaa atctcattta
     4741 gcctgttagc aagcttgacg atgtggcaaa tttatacggt tttatctgta ttagtgcctt
     4801 tgttaaatat tttaagtaaa aatgaaaatg actgtcatgc atccaacatt attaacaagc
     4861 taaagggtga acagaattag ctatgtgatt tttagtgtaa actgctttta actcgtatta
     4921 aaaggccact cttttaatac ctcctaaaat ccctgtacct atcaatagat acagggattt
     4981 tttatgtcca ccaaattcaa ccaattcatc aaccgcgtcc tctcccacga gggcggttac
     5041 gtcaatcatc ccaaagaccc cggcggcgaa accaattggg gcatcactaa gcgcaccgca
     5101 caggcaaacg gctacaacgg ctccatgcgc gccatgacgc gtgaacaggc tatcggcatt
     5161 taccgaaagg cgttttggga gcgttaccgc gccgaccaaa tgccggaagc ggtcgcgttc
     5221 caattttttg atgcctgcgt caaccacggt tacggcaatg ccgtccgtat gctgcaacgc
     5281 gccgcaggcg tggcggacga tggaatcatc ggcgaaatca gcctcaaagc catcaattcc
     5341 ctccccgaaa acgacctttt attgcggttc aacgccgagc gtctggtctt ttataccaag
     5401 ctcagtacgt tcacctcttt cggcaagggc tgggtacgcc gtgtggcgca aaacctgatt
     5461 cacgcgtctg cagataacac tgattaaagg gagacaaacc atgtcaaaaa agtcactcat
     5521 cgccctaatg accgcagcca tgcagcccga tttcagccac agcgacctag gcattcgcta
     5581 cgccatgccg actcagggat gttggacgca agcccaccgc aagagcgggg tagccgccgc
     5641 gaaacgcgca gccaaaaaaa agcgtcgcaa ataactgcct ttttccgatg gctgggcggc
     5701 ttggtctcta atccggccac aggaaaaatc agccatacca aactttgggc aaacgtcgcc
     5761 gcagccgcca tgacttggaa gttcgtgcag gcggcggacg cgcccgaatg gctttggtgg
     5821 gcatacggcg cgatggtcgg cgggtatgca ttaatcaaac gcggcatcgc cgtcgtaccg
     5881 cagttggcgg aagtcaaaaa atccgcaaat caggaagggg ggcggcaatg attgaatttg
     5941 tccgagccaa aaaacggctg ctttgggcat ttgtgctttt gcttgtgtgg acgtgcggtt
     6001 accgatacgc cgccgacaag gccgaagcgg aacaaaccgc cctgattgcc acctatcggc
     6061 attcttctat ggttgcggcg gaacaatatg ccttgcagct taaaaaagcg caggacgaaa
     6121 ggcagcggtg gtacgacttt tcccaaaaac aaggaagaaa gcccgtgaaa aaacagtatc
     6181 cgccgcaaac gaaaaaagcc ggctatctga aaaccaagga agaactgctt gcggaattgg
     6241 cttgccttac agcggaaatg gctgccctaa aaaagcccga tgccttaatc catgggaaag
     6301 aagtgcggca gaaagaacgc aactcgtcgc agggttaagg caatgccatc cgttgaactg
     6361 ctgttggaga ttgtcggact gccacgcggc accttttatt accaattggc cgtccaatcg
     6421 gcagaagaca aatatgccga tttgaaacag catatccatg atatttatcg acgacataag
     6481 ggaagatacg gctaccggag gattgcagca gccatccttc atgcaggaac accggtcaat
     6541 cacaagaaag tcagccgtct gatggcgaag acggggctga aggcagtgat acggcggcgc
     6601 aaataccgct cgttcaaagg agaagtcggc aaaattgcgc cgaatatcct gcgacgctgt
     6661 ttccatgcag aaaagccgaa tgagaaatgg gtaacggacg ttaccgagtt caatgtaggc
     6721 ggagaaaaga tatacctttc tccgattatg gatttgttta acggggaaat cgtcagttac
     6781 cgtattcaaa tccgcccgac tttcgatttg gccggcgaga tactgaaagg tgcgccggag
     6841 aaaccgggat cgtctgaaaa gccgatactg cattcggatc aaggttggca atatcagatg
     6901 tttttttata gtggattaaa tttaaatcag gacaaggcga tgaagccgca gacagtacaa
     6961 atagtacggc aaggcgaggc aacgccgtac tggtttaaat ttaatccact atacaacaac
     7021 gatagaatca agttgaaatt aaaagggctg agccttgttc agtacagaat tcagtccctg
     7081 aaagccgctt gattaaactg tccgactttt tggggtcagt tcggcttcgg cattttttta
     7141 tccgtttggg ggtaacttgt ttggaaagct gcaagcttat aaataaagga ttacatttaa
     7201 gttttgggta acctttttaa aaaaatgcgt gatgactttt gcatttttaa ggcgtttttt
     7261 ggggtaattc gtgaaaagtt accccaaaag ttaccccata aatggcgaaa actcaagcat
     7321 acgccagcat attcaaggca acaaaaaagc cttgaaactg ttgaagttca aggctttttt
     7381 gtgttgcagg atgctgctgg aaatagggta tggtggaggc ggggggaatc gaacccccgt
     7441 ccgaaagtcc tctacaaagc gttctacata cttagttgtg tctatttgaa aatcttattt
     7501 ccatcatgcc gaccaacagg ccttttggaa accagttacc ttaaatctca tttcctgcca
     7561 agtaacccgg taggaaacca gtcaatgtaa gatgacgttg cggtggcttt cgccacacag
     7621 cccattgacc gactgctgca acggctagcc ttaagcggct aaagcgtaag tttcgtcgtt
     7681 tgcgactatt tgaattcagt gttttacggg aatctgagac cccggtatgc ccgcatctgc
     7741 ttcgcaaccc ccgtcgaaac caaggtcgcc cccagaaatg gtttgcaaat tatacggata
     7801 ttgtgcggtg ctgccaagtc tgtcggagaa atttgtcagt cttgctgcct taatttgcgt
     7861 ttgagcagga tgcggacgca gccgtcgttg ccttcccggg gttcggcgta ggcgagtacg
     7921 tcggggtgtt gcatcagcca gtttcgggtc atatttttca gaacgggttt gtagcctttg
     7981 gaacctaatc cgctgccgtg gatgatttcg ccgcatacgc cgcgtttttg ggtgaatgcg
     8041 atgaattcgt tgaggacttt ttgggcttct tcctgtgtgt agccgtgcag gtcgacatcg
     8101 gtaacgacgg gatagtatcc gtttttcagg cgttggatgt cgttttttcc ctgtccgttt
     8161 ttgctgaagc tggcgggcgg gtcgttgtat gtgctgccga tgtagaagta gttttcttcg
     8221 tcggcgcggt tgtctttggg acggactttg atgggggttt tgtcgggcgg cgcataatat
     8281 tgctgccggt tttttaatgg ggagacttgt ccgactgctt gtgaaaaatc gaaatcctgt
     8341 tcttgttttt gcttgttttt ttcggctgcc tgtttttctg ccgcttcttt ttgggcttgt
     8401 ttgcccagtt gtttgaggat gttttggaag tcggtattca tattttttcc tgttatttgt
     8461 ccgatggctg tttcgggcgg ggttttaatt tgccggaatg tttgccaatc gggggaggat
     8521 gattttgttg cctgcgtatg ttttttgaaa gtgtgattgt atatcaaaaa gaaatgcggc
     8581 aaccgtcggc agtgttgatt gccggaaatg cggaccggtc gaaccgatat gcccgaacgc
     8641 ctgataaagt tttaaaaacc tgccttgcga agcaggctga cgtgttttgc caatcttgaa
     8701 ttgccggaaa cgcgaaacac ggaaatctga t
//
LOCUS       DAXHSF010000077         8699 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.077, whole genome shotgun sequence.
ACCESSION   DAXHSF010000077 DAXHSF010000000
VERSION     DAXHSF010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8699
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.077"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            191..1726
                     /locus_tag="ACFM8S_001256"
     CDS             191..1726
                     /locus_tag="ACFM8S_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFC6314324.1"
                     /translation="MSDSKTKERATFGTRRAFMIAAIGSAVGLGNIWRFPYIAFENGG
                     GAFILPYLVALLTAGIPLLLLDYAIGHRYRGSAPLAFRRLGRWFEPVGWWNVMTNIVI
                     CIYYAVIIGWAASYTYYSVNAAWGADPQGFFFKDFLQMAGPEALGLDFVGKVAGPLAG
                     VWVFTAAIMALGVQKGVARASSFFMPLLLVMFLIMVGISLTLPGAAKGLDALFTPDWS
                     KLADSKVWVAAYGQIFFSLSICFGIMVTYSSYLKKKTDLGGTGLVVGFANSSFELLAG
                     IGVFAALGFMAQAGGKAVNEVASGGIGLAFIAFPTIINQAPMGWLIGILFFGSLVFAG
                     VTSMISILEVIVAAIQDKLNIGRVNATLLVCIPMGIVSTLLFGTATGLPVLDVMDKFV
                     NTYGIVAAGFVYVAAIIISGRLPELRKHLNALSSIRIGGLWTVCVVVTVVMLGYMLFK
                     DTSGLMEKNYEGYPDGFLNIFGWGMSAALVVFGLLLSLLPWKHGQDFNVKDEHEHEQG
                     DEK"
     gene            1723..1815
                     /locus_tag="ACFM8S_001257"
     CDS             1723..1815
                     /locus_tag="ACFM8S_001257"
                     /inference="COORDINATES: protein motif:HMM:NF033493.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HFC6314325.1"
                     /translation="MSTSAIVMMIVSIVIIWGGLLLSLLRLPNE"
     gene            complement(1875..3095)
                     /gene="lysA"
                     /locus_tag="ACFM8S_001258"
     CDS             complement(1875..3095)
                     /gene="lysA"
                     /locus_tag="ACFM8S_001258"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFC6314326.1"
                     /translation="MTLFCEQVPYPRLAEEFGTPLYVYSQSALTEAFEHYQTAFAELS
                     PLVCYAVKANGNLSIIKHFASLGSGFDIVSGGELVRVLAAGGDAAKTIFSGVGKSEAE
                     IEFALNAGVKCFNMESIPEIDRIQKVAERLGKTASVSLRVNPDVDAKTHPYISTGLKA
                     NKFGIAYADALEAYRHAAQQPNLKIIGIDCHIGSQLTDLSPLVEACERILILVDTLVA
                     EGIVLEHLDLGGGVGIVYQDESVPDLGAYAQAVQKLIGTRRLKLILEPGRSLVGNAGS
                     LLTRVEFVKHGEEKNFVMVDAAMNDLMRPALYDAYHHIEAVETKNIATLTANIVGPIC
                     ETGDFLGKDRTIACEEGDLLLIRSAGAYGASMASNYNARNRAAEVLVDGGGYRLIRRR
                     ETLEQQMANELACL"
     gene            complement(3106..3276)
                     /locus_tag="ACFM8S_001259"
     CDS             complement(3106..3276)
                     /locus_tag="ACFM8S_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFC6314327.1"
                     /translation="MKYGVFFAAATALLLSACGYKGDLYLPKEGDKARFGVIQTGLQL
                     QSKPQSAPQTQK"
     gene            3347..3670
                     /gene="cyaY"
                     /locus_tag="ACFM8S_001260"
     CDS             3347..3670
                     /gene="cyaY"
                     /locus_tag="ACFM8S_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFC6314328.1"
                     /translation="MMTESEFIRMSEELFEHIEDQIDENGWDFDCQFAGNVLTIEAGD
                     GTQIIVNRHTPNQELWIAAKSGGYHFAEQNGKWLATRDSRDFYDVLNEALSAASGEAV
                     EIEEL"
     gene            3697..4713
                     /locus_tag="ACFM8S_001261"
     CDS             3697..4713
                     /locus_tag="ACFM8S_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFC6314329.1"
                     /translation="MNTRPFYFGLIFIAIIAILANYLGNTDFSHHYHISALIIAILLG
                     MAIGNTIYPQFSTQVEKGVLFAKGALLRTGIVLYGFRLTFGDIADVGLNAVVTDAIML
                     ISTFFFTALLGIRYLKMDKQLVYLTGAGCSICGAAAVMAAEPVTKAESHKVSVAIAVV
                     VIFGTLAIFTYPLFYTWSQHLINAHQFGIYVGSSVHEVAQVYAIGENIDPIVANTAVI
                     SKMIRVMMLAPFLLMLSWLLTRSNGVSENTSHKITIPWFAVLFIGVAIFNSFDLLPKE
                     LVKLFVEIDSFLLISSMAALGLTTQASAIKKAGLKPFVLGILTYLWLVVGGFLVNYGI
                     SKLI"
     gene            4739..5158
                     /locus_tag="ACFM8S_001262"
     CDS             4739..5158
                     /locus_tag="ACFM8S_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HFC6314330.1"
                     /translation="MAQLPLYLTSEIKDFTVGTPKVLESFSKHIPYGVVFEDDGDTGY
                     FYAASQDGILDALHIYNVEDVSDKHIPNHVLILWDDACTIAALSINDYIHAVYDFVEQ
                     AGYCRNGFPETGGEWVKVENRVLDDELLDKILSRKST"
     gene            5180..5686
                     /gene="luxS"
                     /locus_tag="ACFM8S_001263"
     CDS             5180..5686
                     /gene="luxS"
                     /locus_tag="ACFM8S_001263"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFC6314331.1"
                     /translation="MPLLDSFKVDHTRMHAPAVRVAKTMTTPKGDTITVFDLRFCVPN
                     KEILPEKGIHTLEHLFAGFMRDHLNGNGVEIIDISPMGCRTGFYMSLIGTPSEQQVAD
                     AWLASMQDVLNVKDQSKIPELNEYQCGTYQMHSLAEAQQIAQNVLARKVAVNKNEELT
                     LDEGLLNA"
     gene            5832..8627
                     /gene="polA"
                     /locus_tag="ACFM8S_001264"
     CDS             5832..8627
                     /gene="polA"
                     /locus_tag="ACFM8S_001264"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFC6314332.1"
                     /translation="MSNRPTLLLVDGSSYLYRAYHAMGQNLTAPDGAPTGALYGVLNM
                     LRRLRSEYPHDYCAVVFDAKGKNFRHQMFEEYKATRPPMPDDLRPQAEALPDLVRLTG
                     WPVLVIGQVEADDVIGTLAKQGAEHGLRVIVSTGDKDMAQLVDERVTLVNTMSSETLD
                     IEGVKAKFGVRPDQIRDYLALMGDKVDNVPGVEKCGPKTAVKWLEAYGSLAGVMEHAS
                     EIKGKVGENLRAALPRLPLSYDLVTIKTDVDLHAELSDGIESLRRTTPKWAQLVVDFK
                     RWGFRTWLKEAESNMNTGSTDDLFGSDSIGEQAALNAEMPSEKQAEKATVPERLDYQA
                     VTTEAQFAALLDKLAQADTIGIDTETTSLDAMNAALVGISIAFQAGEAVYIPVGHSLT
                     AAPEQLDLQYVLGRLKPHLGNPALKKIGQNLKYDQHVFANYGIALNGIAGDAMLASYI
                     IESHLGHGLDELSGRWLGLETITYESLCGKGAKQIGFADVAIGQATEYAAQDADFALR
                     LEAHLRAQMDEKQLEMYEKMELPVAQVLFEMERNGVQIDRAELARQSAELGAELMKLE
                     QQAYAAAGQPFNLNSPKQLQEILFDKMGIPTKGLKKTAKGGISTNEAVLEQLAPDYPL
                     PKIILQNRSLAKLKSTYTDKLPEMISPRDNRVHTTYAQAVAITGRLASNNPNLQNIPI
                     RTAEGRRVRRAFTAPQGSVIVSADYSQIELRIMAHLSGDKTLIAAFQNGEDVHRRTAA
                     EVFGTAPENVSSEQRRYAKTINFGLIYGMGQYGLAKSLGIDNLSAKNFIDRYFARYPG
                     VAEYMRRTKEQAAAQGYVETLFGRRLYLPDIRNKNANARAGAERAAINAPMQGTASDL
                     IKRAMIDVSHWLDSAGLKTKLIMQVHDELVLEVPEAELDLVKEKLPQIMAKVDEGMLK
                     VPLVAEVGVGENWEEAH"
BASE COUNT         2053 a         2239 c         2289 g         2118 t
ORIGIN      
        1 aaatttttcg ttttcaagcc ttcaccgctt gccatcagcg ttaaattttt ttacgataag
       61 cacatagatt gtaaacaatc ggccacaagc cggtttgttt tttcagaaga cattatccct
      121 gtcagacgct atttctatat atttcgccta taatggcttg tttttaataa ataattcaag
      181 aggtatcaac gtgtctgatt ccaagacgaa agaacgcgcc acattcggca cgcgccgcgc
      241 gtttatgatt gccgccatcg gatccgccgt cggcttgggc aatatttggc gtttccccta
      301 tattgctttt gaaaacggcg gcggcgcgtt catcctgccc tatctggtcg cgcttctgac
      361 ggcgggcatc ccgctgctgc tgctcgatta tgccatcggc caccgttacc gtggttctgc
      421 gcccttggct ttccgccgcc tcggacgatg gtttgagccg gtcggctggt ggaacgtgat
      481 gaccaatatc gtcatctgca tctattacgc ggtaattatc ggttgggcgg caagctatac
      541 ctattattcg gtcaacgccg cctggggtgc ggatccgcag ggttttttct ttaaggactt
      601 cctgcaaatg gcgggcccgg aagccttggg tttggatttt gtcggcaaag tcgccggtcc
      661 tttggcgggc gtgtgggttt ttaccgccgc cattatggcg ttgggcgtgc aaaagggcgt
      721 ggcgcgcgcc tcgtcgttct ttatgccgct gcttttggtg atgtttttga ttatggtcgg
      781 catttcacta accctgccgg gtgcggcaaa gggcttggac gcattgttta cgcccgactg
      841 gtcgaaactc gccgattcca aggtctgggt ggcggcatac gggcagattt tcttttcgct
      901 ttccatctgc ttcggcatta tggttaccta ttcttcttat ttgaagaaaa aaaccgactt
      961 gggcggaacg gggctggtgg tcggttttgc caacagcagc tttgaactgc tcgcgggcat
     1021 cggcgtgttt gccgcattgg gctttatggc gcaggcgggc ggtaaggcgg tcaacgaggt
     1081 tgcctcaggc ggcatcggtt tggcgtttat cgcctttccg accattatca accaggcacc
     1141 gatgggctgg ctgatcggca tattgttttt cggttcgctg gtattcgccg gcgttacgtc
     1201 gatgatttcc atccttgaag tgattgtggc ggcgattcag gacaagctga acatcgggcg
     1261 cgtcaacgcc acgctgctgg tctgcattcc gatgggcatt gtttccacgc tgctgttcgg
     1321 tacggcgacg gggctgccgg ttttggacgt gatggacaaa ttcgtcaaca cctacggcat
     1381 tgttgccgcc ggctttgttt atgttgccgc catcatcatc agcggcaggc tgccggaatt
     1441 acgcaagcac ctgaacgcct tgtcctccat ccgcatcggc ggcttgtgga cggtctgcgt
     1501 cgtggttacc gtcgtgatgc tcggctatat gctgtttaaa gataccagcg gcctgatgga
     1561 gaaaaattac gaaggttatc cggatggttt cctcaatatt ttcggttggg ggatgtcggc
     1621 ggcgttggtc gtgttcgggc tgctgctgtc gttgctgcct tggaaacacg gtcaggattt
     1681 caacgtcaaa gacgaacacg aacatgaaca aggagacgaa aaatgagtac ttccgccatt
     1741 gtgatgatga ttgtctcaat cgtgataatc tggggagggc tgctgctttc cctgttaagg
     1801 ctgccgaacg agtaagcctt tagagcgtta aaaatgccgt ctgaaccgct tcagacggca
     1861 ttttgatgtt cggcttacag gcaggcgagt tcgtttgcca tttgctgttc caaggtttcg
     1921 cgccggcgga tgagtcggta tccgccgccg tcaaccaata cctccgccgc acggttgcgc
     1981 gcgttgtaat tgctcgccat gctggcaccg tatgcgcccg cgctgcggat aagcagcaaa
     2041 tcgccttctt cgcaggcgat ggtgcggtct ttgccgagga agtcgccggt ttcacaaatc
     2101 gggccgacga tgttggcggt cagcgtcgcg atgtttttgg tttcgaccgc ttcgatgtgg
     2161 tggtaggcat cataaagtgc cggacgcatt aaatcgttca tcgccgcatc gaccatcaca
     2221 aagtttttct cttcgccgtg tttgacgaat tcaacgcgcg tcaataacga acctgcgtta
     2281 ccgaccaagc tacgacctgg ttcgaggatg agtttcagac ggcgtgtgcc gatcagtttt
     2341 tgaaccgctt gggcatatgc gcccaaatcg gggacgcttt cgtcttgata aacgatgccg
     2401 acaccgccgc ccaagtccaa atgttccaaa acaatgcctt cggcgacaag agtgtcaacc
     2461 aaaatcaaaa tgcgttcgca ggcttcgacc agcgggctta agtcggtcag ttgcgaaccg
     2521 atgtggcagt cgatgccgat gattttcaaa ttgggctgtt gtgcggcatg gcggtaggct
     2581 tcgagcgcgt cggcgtaggc gatgccgaat ttgttggctt tcagtcctgt ggagatgtag
     2641 ggatgggttt tcgcatcgac atcggggttg acgcgcaggg agacggacgc ggttttgccc
     2701 aagcgttcgg caaccttttg aatgcggtcg atttcgggaa tgctttccat attgaagcat
     2761 ttcacgcccg cattcagcgc aaactcgatt tccgcctcgc ttttgcctac gcctgaaaag
     2821 atggttttcg ccgcgtcgcc gcctgccgcc aaaacgcgta ccaattctcc gccggacaca
     2881 atgtcgaaac cgctgcccag agaagcaaag tgtttgatga tgctcaggtt gccattggct
     2941 ttaaccgcat agcagacgag cggggagagc tcggcaaagg cagtttggta gtgttcaaat
     3001 gcttcggtca gcgcggattg gctgtacaca taaagcggcg tgccgaattc ttcggcaagg
     3061 cgggggtagg ggacttgttc gcaaaatagg gtcatgtttt cgttttcatt tttgggtttg
     3121 tggagcggat tgtggtttgc tttgaagttg caaaccggtt tggattacgc cgaaacgcgc
     3181 cttgtcgcct tctttgggca ggtagaggtc gcctttgtaa ccgcaggccg agagcaggag
     3241 ggcggttgcc gccgcaaaaa atacgccgta tttcatcggt aaacttcctt cataagcgcg
     3301 aatgtggcaa gattcggtat cttaaacaaa aaacacgcaa aaagccatga tgaccgaaag
     3361 cgaattcatc cgcatgagcg aagaattgtt cgaacacatc gaagaccaaa tcgacgaaaa
     3421 tggctgggat ttcgactgcc agtttgccgg aaacgtcttg accatcgaag ccggagacgg
     3481 cacgcaaatc atcgtcaacc gccacacgcc caatcaggaa ttgtggattg ccgccaaaag
     3541 cggcggctac catttcgccg agcaaaacgg caaatggctg gcaacacgcg acagccgcga
     3601 tttctacgac gttttaaacg aagcactgag cgcggcttcg ggcgaagcgg tagagattga
     3661 ggaattgtga tttgggtgtt atcacggaaa gaaaaaatga acacacgtcc cttttatttc
     3721 ggacttatat ttatcgcgat tatcgctata cttgctaact atttaggaaa cactgatttt
     3781 tcccatcatt atcatatcag tgctttaatt attgctatct tgctgggaat ggcaatcggc
     3841 aataccattt atccgcaatt ttcaacacaa gtggaaaaag gcgttttgtt tgccaaaggc
     3901 gcgcttcttc gcactggcat tgtgctgtat ggtttccgcc tcacttttgg cgatattgcc
     3961 gatgtaggat taaatgctgt cgttaccgat gcaatcatgc taatttcaac cttctttttt
     4021 accgcacttt taggcattcg ttatctaaaa atggataaac aattggttta tctcactggg
     4081 gcaggttgca gcatttgcgg tgcggcagca gtgatggcgg cagagcctgt taccaaagca
     4141 gaatcccata aagtttcagt ggcgattgcc gtagtggtca ttttcgggac gcttgctatt
     4201 tttacttacc ccttgttcta cacgtggtca caacatttaa ttaacgccca tcaattcggt
     4261 atttatgttg gttctagtgt acacgaagtg gctcaagttt atgcgattgg ggaaaatatt
     4321 gatcctatcg tggcgaatac tgccgtcatt tccaaaatga tccgagtgat gatgctcgcc
     4381 ccctttttat taatgctttc ttggttatta acacgtagta atggagtatc agaaaataca
     4441 tcacacaaaa ttacaattcc ttggtttgct gtacttttta ttggtgttgc catttttaat
     4501 tcttttgatt tattaccaaa agaactcgtg aaattattcg ttgaaatcga ttctttctta
     4561 ttaatttcat caatggctgc gcttggctta acgacgcaag caagcgcaat caaaaaggca
     4621 ggattaaaac cgtttgtttt aggaatacta acttatttat ggctagtggt tggtggattt
     4681 ttagtgaact atggaatatc aaaattaata taaaattcac taaagagagc gttacccaat
     4741 ggcacaatta ccgctatatc tgacttctga aatcaaagac tttactgtcg gcacgcctaa
     4801 agttttggaa tcattttcca aacatatccc ttatggtgtt gtctttgaag acgacggcga
     4861 cacgggctac ttctatgccg cttcgcaaga cgggattcta gatgccttgc acatctacaa
     4921 tgtcgaagat gtatccgaca aacatatccc caatcatgtc ttgattttat gggatgatgc
     4981 ctgcaccata gccgcattga gtatcaacga ctacattcat gccgtctatg attttgtcga
     5041 acaggcagga tattgccgca acggcttccc tgaaacaggc ggcgaatggg tgaaagtcga
     5101 aaaccgcgtc ttggacgatg aattgctgga caaaatctta tcccgaaaat ctacataacc
     5161 tactcaaaag gataacaaaa tgcccctgtt agacagtttc aaagtcgacc acacccgtat
     5221 gcacgcccct gccgtacgcg tggcgaaaac catgaccacg cccaaaggcg ataccattac
     5281 cgtattcgac ctgcgctttt gcgttcctaa caaagaaatc ctgcctgaaa aaggcataca
     5341 cacgctggag catttgttcg caggctttat gcgcgaccac ttaaacggca acggcgtcga
     5401 aatcatcgac atttccccga tgggctgccg caccggtttt tatatgagcc ttatcggcac
     5461 gccttccgaa cagcaggtcg ccgatgcgtg gctggcttcg atgcaggatg ttttgaatgt
     5521 caaagaccaa agcaaaatcc ccgagttgaa cgaataccaa tgcggcactt atcaaatgca
     5581 ctcgctcgcc gaagcgcaac aaatcgcgca aaacgtgttg gcgcgcaaag tggcggtgaa
     5641 caaaaatgaa gagctgacgc tggatgaagg gctgctgaac gcctaatccg ccaaaaatgc
     5701 cgtctgaaca agggtttcag acggcatttg ccttttccgt tataatctag ggttgtgcgg
     5761 gggcgggttt taagccggca tcgtccttcc ctattttttt ctgtccctta tcggttttaa
     5821 gcgggttttt tatgtccaac agacctacac tcctcctcgt tgacggatcg tcctacctct
     5881 accgtgcgta tcacgcgatg gggcaaaacc tgaccgcacc cgacggtgca ccgacgggtg
     5941 cgctgtatgg cgtattgaat atgttgcgcc gtttgcggtc ggaatatccg cacgattatt
     6001 gcgcggtggt ttttgatgcg aaaggcaaaa atttccgcca tcaaatgttt gaagaataca
     6061 aggcgacgcg cccgccgatg cccgacgatt tgcgcccgca ggcggaagca ctgccggatt
     6121 tagtgcgcct gacaggctgg ccggtattgg tgattgggca ggtggaggcg gacgatgtga
     6181 tcggcacgct ggcgaaacag ggggcggaac atggtttgcg agtcattgtt tcgaccggcg
     6241 ataaggacat ggcgcagttg gtggatgagc gcgttacgct ggtgaacacg atgagcagcg
     6301 aaacgctgga cattgaaggc gtgaaggcaa aattcggcgt gcgccccgac caaatccgcg
     6361 attatctcgc gctgatgggc gacaaggtgg acaacgtgcc gggcgtggaa aaatgcggcc
     6421 cgaaaacggc ggtgaaatgg ctggaagcct acggttcgct ggctggtgtg atggaacacg
     6481 cttcggaaat caagggcaaa gtgggcgaga atctgcgtgc cgccctgccg cgcctgccgc
     6541 tgtcgtatga cttagtaacg attaaaaccg atgtggactt gcacgccgag ctttcagacg
     6601 gcatcgaaag cctgcgccgt actacgccga aatgggcgca gctggttgtc gatttcaaac
     6661 gctggggctt ccgcacctgg ctgaaagaag cggaatcaaa catgaatacc ggctcgaccg
     6721 atgatttgtt cggcagcgac agcatcggcg agcaggcggc tttgaatgcg gaaatgccgt
     6781 ctgaaaaaca agctgaaaaa gccaccgtcc ccgaaaggct ggattatcaa gccgttacca
     6841 ccgaagcgca gtttgccgct ttgttggaca aactggctca ggcggacaca atcggcatcg
     6901 atacggaaac cacgtcatta gacgcgatga acgccgcgct ggtcggcatc agcattgcgt
     6961 tccaagcagg ggaggcggtt tacatccccg taggacacag cctgaccgcc gcgcctgaac
     7021 agcttgattt acaatacgta ttaggccgtc tgaaaccgca tttgggaaac cccgccctga
     7081 aaaaaatcgg gcaaaacctc aaatacgacc aacacgtttt cgctaactac ggcatcgccc
     7141 tgaacggcat tgccggcgac gccatgctcg cttcctacat catcgagagc catctcggac
     7201 acggattgga cgaattgtcc ggacgctggc tcggcttgga aaccattacc tacgaatcgc
     7261 tgtgcggcaa aggcgcgaag caaatcggtt ttgccgatgt cgccatcggg caggcgaccg
     7321 aatacgccgc ccaagacgcc gatttcgccc tgcgcctcga agcgcacttg cgcgcgcaaa
     7381 tggacgaaaa acagcttgaa atgtatgaaa aaatggagct gcccgtcgcg caggtattgt
     7441 ttgaaatgga acgcaacggc gtgcaaatcg accgcgccga actcgcccgc caaagcgcgg
     7501 aactcggcgc cgagctgatg aagctcgaac aacaagccta tgccgccgca ggccagccgt
     7561 tcaacctcaa ttcgcccaaa cagctgcaag aaatcctgtt cgacaaaatg ggcatcccca
     7621 ccaaaggcct gaaaaaaacc gccaaaggcg gcatttccac caacgaagcc gtgctcgaac
     7681 agctcgcgcc cgactacccc ctgcccaaaa tcatcctgca aaaccgcagc ctggcgaaac
     7741 tcaaatccac ctacaccgac aaactgcccg aaatgatttc gccccgggat aaccgcgtgc
     7801 ataccaccta cgcccaagcc gtcgccatta caggacgctt ggcaagcaac aaccccaacc
     7861 tgcaaaacat ccccatccgc accgccgaag gccgccgtgt gcgccgcgcc tttaccgcac
     7921 cgcaaggcag cgtcatcgtt tccgccgact attcccaaat cgagctgcgc attatggcgc
     7981 acctctccgg cgacaaaacc ctcatcgccg cgttccaaaa cggcgaagac gtacaccgcc
     8041 gcaccgccgc cgaagtgttc ggcacggcgc ccgaaaacgt ctcgtccgag caacgccgct
     8101 acgccaaaac catcaacttc ggcttaattt acggtatggg gcaatacggt ttggcaaaat
     8161 cattgggcat cgacaacctt tccgccaaaa actttatcga ccgctacttc gcccgctacc
     8221 ccggcgtcgc cgaatacatg cggcgcacca aagaacaagc cgccgcccaa ggctacgtcg
     8281 aaaccctgtt cggcagaagg ctctatttgc ccgacatccg caacaaaaac gccaacgccc
     8341 gcgcaggagc cgagcgcgcc gccatcaatg cccccatgca aggcaccgcg tccgacctca
     8401 tcaaacgcgc catgatagac gtgtcccatt ggttggattc agccggcctg aaaaccaaat
     8461 tgattatgca ggtgcatgac gaattggtgt tggaagtacc cgaagccgag ctggatttag
     8521 tcaaagaaaa actgccgcag ataatggcga aagtggatga agggatgttg aaagtgccgc
     8581 tggtggctga ggttggcgta ggggagaatt gggaagaggc acattgattg aaaggtgtta
     8641 tatgctatct ttatttaaat aaaatttaat ttttggtata ttttttctaa atgttccta
//
LOCUS       DAXHSF010000078         8689 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.078, whole genome shotgun sequence.
ACCESSION   DAXHSF010000078 DAXHSF010000000
VERSION     DAXHSF010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8689
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.078"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(642..1721)
                     /gene="apbC"
                     /locus_tag="ACFM8S_001265"
     CDS             complement(642..1721)
                     /gene="apbC"
                     /locus_tag="ACFM8S_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFC6314333.1"
                     /translation="MNIQNIRTLLDTVAVPNTARTLGGEKAVRSVEQRSDGIHIALHF
                     GFPVAHIASETADRIQEILMPLTGDTHIHLSMDTEIGTHKVQPGVTTIKGVKNIIAVA
                     SGKGGVGKSTTTANLAAAMARMGARVGVLDADLYGPSQPTMLGVDDRKPDQKNQKLIP
                     VESSDGIQVMSIGFLVDTDQAVVWRGPMVSQALQQLMFQSEWDEVDYLFIDLPPGTGD
                     IQLTLSQRIPVTGSVIVTTPQDIALIDARKAVDMFRKVNIPILGVLENMSVHICTNCG
                     HSEALFGTDGGKDLAARLNVPLLGQLPLSLPVREAMDGGTPAQLFDEHPAIARIYTDA
                     AFQIALGIADKGKDFSSRFPKIVVE"
     gene            1932..2007
                     /locus_tag="ACFM8S_001266"
     tRNA            1932..2007
                     /locus_tag="ACFM8S_001266"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1965..1967,aa:Lys,seq:ttt)
     gene            2086..2595
                     /locus_tag="ACFM8S_001267"
     CDS             2086..2595
                     /locus_tag="ACFM8S_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HFC6314334.1"
                     /translation="MKKKLLSGIKFAVQTALVFLLVSLFLDWIRKPDEPAGAAGRPLT
                     LLSGQRLTLGQFSRDKAVLVYFWGSWCGVCRYQSPIIDDLAADGVPVVGVAVRSGSAS
                     EVAAYMAKRGLGFPTVNDEDGGLARSWRIVATPAVVLVKNGKMVHYTTGISSYWGLRA
                     RIFQADVFG"
     gene            2938..3378
                     /gene="nadR"
                     /locus_tag="ACFM8S_001268"
     CDS             2938..3378
                     /gene="nadR"
                     /locus_tag="ACFM8S_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family adhesin repressor NadR"
                     /protein_id="HFC6314335.1"
                     /translation="MPTQSKHASINISLIQAREALMTQFRPILNQANITDQQWRIIRL
                     LAENGTLDFQDLANQACILRPSLTGILTRLEKAGLVVRLKPSNDQRRVFLKLTAEGEK
                     LYEEIGEEVDERYDAIEEVLGREKMLLLKDLLAELAKIEDALNS"
     gene            3493..3993
                     /gene="hpaC"
                     /locus_tag="ACFM8S_001269"
     CDS             3493..3993
                     /gene="hpaC"
                     /locus_tag="ACFM8S_001269"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042537 - benzene-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HFC6314336.1"
                     /translation="MADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVTP
                     VTDEPPTVMLCINRSARIIPILSENGSLCINTLADEHQDVAEHFAGLTGLSPEERFAY
                     HIWHRGKTGQLEIEGALAHLHGHIVGKHEIGTHFVFYVRLDEIKNCGCKRPALLYFRR
                     QFRFLD"
     gene            4009..4704
                     /gene="murU"
                     /locus_tag="ACFM8S_001270"
     CDS             4009..4704
                     /gene="murU"
                     /locus_tag="ACFM8S_001270"
                     /EC_number="2.7.7.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramate alpha-1-phosphate
                     uridylyltransferase MurU"
                     /protein_id="HFC6314337.1"
                     /translation="MKAMILAAGRGERMRPLTDTTPKPLLDVAGKPLIGWHLCRLKQA
                     GFTEIVINHAWLGRQIEDALGDGSAYGVNIAYSPEPAGGLETAGGIAQALPLLGGQPF
                     LVVNGDVLTDIDFTAAFQAASSLPEHISAHLWLVENPPHNPDGDFSLLPDGSVRPEVN
                     GGNGLTFSGVGIYRPEMFDGIEAGSVAKLAPVLRGEMRQNRVSGQKHTGLWLDVGTVC
                     RLKEAQALAGAWK"
     gene            complement(4935..5366)
                     /locus_tag="ACFM8S_001271"
     CDS             complement(4935..5366)
                     /locus_tag="ACFM8S_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFC6314338.1"
                     /translation="MSSNAWLFWALASAGFASLTAVFAKMGLQGIDSDFATFIRTLVI
                     LAALVLFLTYTGKWQGVNGFTGRNWTFLILSGLATGASWLAYFKALQLGNASQVAPID
                     KFSLVLVALMAVVFLDERPNTQEWIGLGLVTAGVLVLALKR"
     gene            5541..7217
                     /locus_tag="ACFM8S_001272"
     CDS             5541..7217
                     /locus_tag="ACFM8S_001272"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFC6314339.1"
                     /translation="MSFKTDAEIAQSSTMRPIGEIAAKLGLNADNIEPYGHYKAKINP
                     AEAFKLPQKQGRLILVTAINPTPAGEGKTTVTIGLADALRHIGKDAVIALREPSLGPV
                     FGVKGGAAGGGYAQVLPMEDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELDIDPK
                     RVLWRRVVDMNDRQLRNIIDGMGKPVDGVMRPDGFDITVASEVMAVFCLAKDISDLKE
                     RLGNILVAYAKDGSPVYAKDLKAHGAMAVLLKDAIKPNLVQTIEGTPAFVHGGPFANI
                     AHGCNSVTATRLAKHLADYAVTEAGFGADLGAEKFCDIKCRLAGLKPDAAVVVATVRA
                     LKYNGGVERANLGEENLEALAKGLPNLLKHISNLKNVFGLPVVVALNRFVSDSDAELA
                     MIEKACAEHGVEVSLTEVWGKGGVGGADLARKVVNAIENQPNNFNFSYDVELSIKDKI
                     RAIAQKVYGAEDVDFSAEASAEIASLEKLGLDKMPICMAKTQYSLSDNAKLLGCPEGF
                     RITVRGITVSSGAGFIVALCGNMMKMPGLPKVPAAEKIDVDAEGVIHGLF"
     gene            7331..8422
                     /gene="ychF"
                     /locus_tag="ACFM8S_001273"
     CDS             7331..8422
                     /gene="ychF"
                     /locus_tag="ACFM8S_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFC6314340.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTQSGIEAANYPFCTIEPNVGIV
                     EVPDPRMAELAKIVNPQKMQPAIVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIVN
                     VVRCFDDDNIVHVAGRVDPIADIETIGTELALADLASVAKAIVREEKRARSGDKDAQK
                     LVDLCKKLLPHLDEGKPVRSFGLDAEERAMLKPLFLLTAKPAMYVGNVAEDGFENNPH
                     LDRLKELAAKENAPVVAVCAAMESEIAELEDDEKAEFLAEMGLEEPGLNRLIRAGYDL
                     LGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQVIAYDDFVSLGGEA
                     KAKEAGKMRVEGKEYVVQDGDVMHFLFNV"
BASE COUNT         1957 a         2229 c         2483 g         2020 t
ORIGIN      
        1 gacgggaacg gcgcacgggc attgccggaa tcttttgtgt ttccattatc atactctttt
       61 taatgcgcta ttccaagaat atgatgccct ccggcggcat cccaccctac ggcttgctat
      121 aaaaatacct aaaccgtcat tcccgcaaac ctacattccg tcattcccac gaaagtggga
      181 atctagaatc tcggactttc agataatctt tgaatattgc cgctgtccaa tggtctagat
      241 tcccgcctac gcgggaatga cgggtctttt ataatctttg aatattgctg ttgttctaag
      301 gtctagattc ccgcctgcgc gggaatgacg gtttagaagt tgcccgaaac ctcaaaaaaa
      361 accgaaaccg aacaagccgg attcccgcct gcgcgggaat gacggcggag cggtttctgt
      421 tttttccgat aaatgccgca atctcaaatc ccgtcattcc cacgaaccta cattccgtca
      481 ttcccacgaa agtgggaatc caggacgcgg aatctcaaga aaccgtttac ccgataagtt
      541 tccgtgccga cagacctaga ttcccgccta cgcgggaatg acggcatatc ggcaggcgcg
      601 gggcttggga cggcattgct gttgcggttt cggacgcggc tttattcgac gacaatttta
      661 gggaaccggc tgctgaagtc tttgcctttg tcggcaatgc ccagggcgat ttggaatgcg
      721 gcatcggtgt agattcgggc gatggcgggg tgttcgtcga acagttgcgc cggtgtgccg
      781 ccgtccattg cttcgcgcac gggcaggctt aggggaagct gtccgagcag ggggacgttg
      841 aggcgtgccg ccaaatcttt gccgccgtcc gtgccgaaca gggcttcgct gtgtccgcag
      901 ttggtgcaga tgtggacgga catattttcc aatacgccca aaatcggaat gttgactttt
      961 cggaacatat ccacggcttt gcgcgcgtct atcagggcga tgtcctgcgg cgtggttacg
     1021 atgaccgatc cggttacggg gatgcgttgg gacagcgtga gttggatgtc gcccgtgccg
     1081 gggggcaggt cgatgaaaag ataatccact tcgtcccact cgctttgaaa catcagttgc
     1141 tgcaaggctt ggctgaccat cggcccgcgc cagacgacgg cttggtcggt atcgacgaga
     1201 aagccgatag acatgacctg tatgccgtct gaagattcga cgggaatgag tttttggttt
     1261 ttctgatcgg gtttgcggtc gtccacaccc aacatggtcg gttggctcgg gccgtaaagg
     1321 tcggcatcga gtacgccgac gcgcgcgccc atgcgcgcca ttgcggcggc aaggttggcg
     1381 gtggttgttg atttgcccac gccgcctttg ccggaggcga cggcaatgat gtttttcaca
     1441 cctttgatgg tggtaacgcc aggttggact ttatgtgtgc cgatttccgt gtccatggac
     1501 agatggatgt gtgtgtcgcc tgtcagcggc atcaggattt cctgtatgcg gtcggctgtt
     1561 tctgaggcaa tgtgcgcgac ggggaagccg aaatgcaggg cgatatggat gccgtctgaa
     1621 cgctgttcga ccgaacggac ggccttttcg ccgccgagcg tgcgtgccgt attcggaacg
     1681 gcgacggtgt cgaggagggt gcggatgttt tgtatgttca taatgtgtgt ttctgtgtgc
     1741 agtttgggaa acgggcggaa agtgtacatt gatttcgggc ggtttgcttt tccttgtgcg
     1801 aacttttaca cggtatgcgg cggattgtga aaatccgttg tcaaaatatc ccgtgcaaac
     1861 agtggtctgc aattaagcct gtaaaatttg cttgacgagg taggatggtg ctgtataatg
     1921 cacggcttat cgggtcgtta gctcagccgg tagagcagcg gacttttaat ccgttggtcg
     1981 agcgttcgaa tcgctcacga cccaccagat aaccggaagc caagttttga gacttggctt
     2041 tttgtttgcc tgccggttgc gtgggcgtac cgtttgagag agcgtatgaa aaagaaactg
     2101 ctttcgggca tcaaatttgc cgttcagacg gcattggtgt ttttattggt gtcgctgttt
     2161 ttggattgga tacgcaagcc cgacgaacct gccggtgcgg cagggcggcc tttgaccctg
     2221 ctgtcggggc agcggctgac tttgggacag tttagccggg ataaggcggt tttggtgtat
     2281 ttttggggga gctggtgcgg cgtgtgccgt tatcagtcgc cgataatcga tgatttggcg
     2341 gcggacggcg ttccggtcgt cggcgtggcg gtacgttccg gcagcgcgtc cgaagtggcg
     2401 gcgtatatgg caaagcgggg cttgggcttt ccgacggtca acgatgagga cgggggtttg
     2461 gcaaggtctt ggcggattgt cgcaacgcct gccgtcgttt tggtcaaaaa tgggaaaatg
     2521 gtccattata cgacgggaat cagcagttat tggggcttgc gcgcacgaat ttttcaggcg
     2581 gatgttttcg gttaactttt gtttttgtta aactttacgc tatgtagaga atcaaacggc
     2641 atataccttg ctcgtctgat gcggcggctt ttgttttttc tggaagaaag ttctttcgac
     2701 agtaaatgta atactttgtt gagattgtag ggaaaataga ctaagatata actattaaaa
     2761 tatttttaga taggattatc ggaattaaag tcttttatac ccagtcgtcc gatgcggttt
     2821 atagcgtatt gttgctatat gttcgttttg ttatataacg gttgcatcaa aatttacgcc
     2881 cacaggcttt cccgacggtt tgaaagtttg attttcgata acttggagac ttgaacaatg
     2941 cctacccaat caaaacatgc gtctatcaat atcagtctga tacaggcaag ggaagccctg
     3001 atgacccaat tcaggcctat tctgaatcag gcgaatatta ccgatcagca atggcggatt
     3061 atccgtcttt tggcggaaaa cggcacgctg gactttcaag atttggcgaa tcaggcgtgc
     3121 attttgcgcc ccagcctgac cggtatcctg acccgccttg aaaaagcggg tttggtcgtc
     3181 cgcctgaaac cttccaacga ccaacgacgt gtttttctga agctgactgc cgagggcgag
     3241 aagctgtatg aggaaatcgg cgaagaagtg gacgagcgtt acgacgctat cgaggaagtg
     3301 ctgggccgcg agaaaatgct gctgcttaaa gacctgttgg cagaacttgc caaaatcgag
     3361 gatgcgttga actcgtaata cgccgtaacg cgcggaaacg tccgaccgac ggctttttga
     3421 atcaaaactg ctgcacatgg gggatgcctt gtgtgcagca ttcttatata ggggacagtt
     3481 taaaggggaa aaatggcgga tttgcagaaa acttttcaaa cttcgttccg tgatgcgatg
     3541 gcatcttgtg cggcaggcgt tcatgtcatc acgacagacg gtgcggcagg gcgttacggc
     3601 attacaatga cggcggttac accggttacg gacgagccgc cgaccgtgat gctgtgcatc
     3661 aaccggagtg cgcgaatcat tccgatcctg tcggaaaacg gcagcctctg catcaatacg
     3721 ctggcggacg aacatcagga tgttgccgaa cattttgccg ggctgaccgg gctgtcgccc
     3781 gaagagcggt ttgcctacca catctggcat cgcggcaaaa cgggacaact tgaaatagag
     3841 ggcgcgttgg cgcacctgca cgggcatatt gtcggcaaac atgaaatcgg cacgcatttt
     3901 gtgttttacg tcaggctcga cgaaatcaaa aactgcgggt gcaaacgccc cgcgctgctg
     3961 tatttcagac ggcagtttag atttttagac tgatattcgg acagatatat gaaagcgatg
     4021 atactggcgg caggacgcgg cgagcgtatg cgccctttga ccgataccac tccgaagccg
     4081 ctgctcgatg tggcgggtaa gcctctaatc ggttggcacc tatgccgtct gaagcaggcg
     4141 gggtttaccg aaatcgtcat caaccacgct tggctgggcc ggcagataga agatgctttg
     4201 ggcgacggct cggcttatgg cgtgaacatc gcctattcgc ccgaacccgc aggcggtttg
     4261 gaaacggcag gcggcatcgc gcaggcattg ccgctgttgg gtgggcagcc gtttttggtg
     4321 gtcaacggcg acgtgctgac cgacatcgat tttaccgccg cgtttcaggc ggcatcgtcc
     4381 ctgccggaac atatttccgc ccatctgtgg ctggtggaaa atccgccgca caaccccgac
     4441 ggcgattttt ccctgctgcc cgacggcagc gtgcggccgg aagtaaatgg cggcaacgga
     4501 ttgacattca gcggcgtggg tatttaccgt cctgaaatgt ttgacggaat cgaagcgggc
     4561 agtgtggcga aactcgcgcc cgtattgcgt ggcgaaatgc ggcaaaaccg cgtgagcggt
     4621 cagaagcata cgggcttgtg gctggatgtc ggcacggtat gccgtctgaa agaggctcaa
     4681 gcccttgcag gggcttggaa gtgaaataaa acggtggctt gccgagccgg ctgccgaacg
     4741 gttccgaccg gatgtccggc atattttcgt ggaggcgtga aaatagggcg ttcggggctt
     4801 tccgtccaac aaatgccatc tgaagcgcgg cggtttgccg tcggcgcggt gtcggatttt
     4861 attgggtaac tttgttcaga ccgccgcccg tcagattgtc aaaacccggt ttcaggcggt
     4921 atggcggatt cggtttaacg tttcaacgcc agcaccaaca cgcccgccgt taccagcccc
     4981 aagcctatcc attcctgcgt gttcgggcgt tcgtccaaga aaaccaccgc catcagcgcg
     5041 accaagacca ggctgaattt gtcgatgggg gcgacttgcg aggcgttgcc cagttgcagg
     5101 gctttgaagt aggcgagcca ggatgcgccg gtagcaaggc cggataggat aaggaatgtc
     5161 cagttgcgcc ccgtaaagcc gttcacaccc tgccatttgc cggtgtaggt caaaaacaat
     5221 accaaagcgg cgaggatcac caaggtgcgg ataaaggtgg cgaaatcgga atcgatgccc
     5281 tgtaaaccca ttttggcgaa aacggcggtc aatgaggcga agcctgccga tgccaatgcc
     5341 caaaacagcc atgcgttgct gctcatgttt tctccttgga ttgtgaacaa tatgaacggt
     5401 atttttgttg cagcgtcaaa aatttcactg cgggtttggt gcggataacg ttataatatg
     5461 cctgatatta ttttcaatcc acctgtttgt cgcctgatgc tttcagacgg catgtccctc
     5521 ctcatttcta aaggaaaatc atgagcttca aaaccgatgc cgaaatcgcc caatcctcca
     5581 ccatgcgccc gattggcgaa attgccgcca agcttggtct gaatgccgac aacattgagc
     5641 cttacggtca ttacaaggcg aaaatcaatc ctgccgaagc gttcaaactg ccgcaaaaac
     5701 agggcaggct gattttggtt accgccatca acccgactcc ggcgggcgaa ggcaaaacca
     5761 ccgtaaccat cggtttggcg gacgcgttgc gccacatcgg caaagatgcc gtgattgccc
     5821 tgcgcgaacc ttctctgggg ccggtgttcg gcgtgaaagg cggcgcggca ggcggcggct
     5881 atgcccaagt tttgccgatg gaagacatca acctgcactt caccggagat tttcacgcca
     5941 tcggtgcggc aaataatctg cttgccgcga tgctcgacaa ccatatctat cagggcaacg
     6001 agttggacat cgaccccaaa cgcgtgctgt ggcggcgcgt ggtcgatatg aacgaccgcc
     6061 agttgcgcaa catcatcgac ggcatgggta aacccgttga cggtgtgatg cgccccgacg
     6121 gttttgatat caccgttgcc tccgaagtga tggcggtatt ctgccttgcc aaagacatca
     6181 gcgatttgaa agagcgtttg ggcaacatcc tcgtcgccta cgccaaagac ggcagccccg
     6241 tttacgccaa agatttgaag gcacacggcg cgatggcggt cctgctcaaa gatgcgatta
     6301 agcccaattt ggtgcaaacc atcgaaggca cgcccgcctt cgtacacggc ggcccgttcg
     6361 ccaacatcgc ccacggctgc aactccgtaa ccgcaacccg tctggcgaaa caccttgccg
     6421 attacgccgt aaccgaagca ggcttcggcg cggatttggg cgcggaaaaa ttctgcgaca
     6481 tcaaatgccg ccttgccggt ttgaaacctg atgcggcggt cgtcgtggcg actgtccgcg
     6541 ctttgaaata caacggcggc gtggaacgtg ccaacctcgg tgaagaaaac ctcgaagcct
     6601 tggcaaaagg tttgcccaac ctgctgaaac acatttccaa cctgaaaaac gtattcggac
     6661 tgcccgtcgt cgttgcgctc aaccgcttcg tgtccgactc cgatgccgag ttggcgatga
     6721 ttgaaaaagc ctgtgccgaa cacggcgttg aagtttcctt gaccgaagtg tggggcaaag
     6781 gcggcgtggg cggtgcagac ttggcgcgca aagtcgtcaa cgccatcgaa aaccaaccga
     6841 acaacttcaa tttttcctac gatgttgagc tgagtattaa agacaaaatc cgtgcgattg
     6901 cccaaaaagt gtacggcgcg gaagatgttg atttcagcgc ggaagcgtct gccgaaatcg
     6961 cctcgctgga aaaactgggc ttggacaaaa tgccgatttg catggcgaaa acccaatatt
     7021 ctttgagcga caacgccaaa ctcttgggct gccctgaagg cttccgcatt accgtgcgcg
     7081 gcattaccgt ttcttccggt gctggtttca tcgttgcgtt gtgcggcaat atgatgaaaa
     7141 tgccgggtct gcccaaagtt cccgccgccg agaaaatcga cgtggacgca gaaggcgtga
     7201 ttcacggctt gttctgaacg gtttttgaaa ccagatgccg tctgaagccg tttcagacgg
     7261 cattttttcg gaacgcgggc ggcggtatgc tataatccgc cgttaaattt ctctattttc
     7321 aggaaaaaac atgagtttga aatgcggcat cgtcggtttg cccaacgtcg gcaaatccac
     7381 cctttttaac gcgctgaccc aatcgggtat cgaagcggca aactatcctt tctgtaccat
     7441 cgaacccaac gtcggcatcg tcgaagtccc cgatccgcgt atggccgaat tggcaaaaat
     7501 cgtcaatccg caaaaaatgc agcctgccat cgtcgaattt gtcgatattg ccggtttggt
     7561 tgcaggcgcg agcaaaggcg agggcttggg caaccagttc cttgccaaca tccgcgaaac
     7621 cgatgcgatt gtgaatgtcg tgcgctgctt tgacgacgac aacatcgtcc acgttgcagg
     7681 ccgcgtcgat ccgattgccg acatcgaaac catcggcaca gagttggcac ttgccgacct
     7741 ggcaagtgtg gcaaaagcca tcgtccgcga agaaaaacgc gcccgttcag gcgacaaaga
     7801 cgcgcaaaag ctggtcgatt tgtgcaaaaa actgctgccg catctggacg aaggcaaacc
     7861 cgtgcgttcc ttcggtttgg acgcggaaga acgcgcgatg ctcaaaccgc tgttcctgct
     7921 gacagccaaa cctgcgatgt atgtgggcaa cgtcgccgaa gacggttttg aaaacaatcc
     7981 gcacctcgac cgcctgaaag aattggcggc aaaagaaaac gcccccgtcg tcgccgtatg
     8041 cgccgcgatg gaaagcgaaa tcgccgaatt ggaagacgac gaaaaagccg aattcctcgc
     8101 cgaaatgggt ttggaagaac ccggcctgaa ccgcctgatc cgcgccggct acgacctctt
     8161 gggcctgcaa acctacttca ccgccggcgt gaaagaagtc cgcgcgtgga cgatacacaa
     8221 aggcgacacc gccccgcaag ccgccggcgt gattcatacg gattttgaac gcggcttcat
     8281 ccgcgcccaa gtgattgcct acgatgattt tgtctcgctc ggcggcgaag ccaaagccaa
     8341 agaagccggc aaaatgcgtg tggaaggcaa agaatacgtc gtgcaggatg gggatgtgat
     8401 gcactttttg tttaacgtgt aacccaaatg cggcaggttt tcagacggct ttgccagaaa
     8461 tgccgtctga agccggtttt ggtggttttc ggcgtttcca taccgccgga atgcggctgc
     8521 atcaaaataa aatcccaccc gcatttccga tttgccctcc ccgattcctg caaaacaaac
     8581 cgcctgcccc gctgttgcaa aaagccgtcc ggcattccga atatcccgta caaaatgacc
     8641 tttcagacgg catttgcgcc gccgcgtttc gagtatagtg gattaactt
//
LOCUS       DAXHSF010000079         8653 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.079, whole genome shotgun sequence.
ACCESSION   DAXHSF010000079 DAXHSF010000000
VERSION     DAXHSF010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8653
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.079"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(239..3091)
                     /gene="gcvP"
                     /locus_tag="ACFM8S_001274"
     CDS             complement(239..3091)
                     /gene="gcvP"
                     /locus_tag="ACFM8S_001274"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFC6314341.1"
                     /translation="MKLSELFNPNEFAAQHLSFGDEAALLETLGEKSMDDFVGNTVPQ
                     SIRMPSELDLPEALTEADALAKLKGIASKNVINKSYIGLGYYPTRVPNVILRNVLENP
                     GWYTAYTPYQAEIAQGRLEALLNFQQVCIDLTGFPVAGASLLDEATAAAEAMAMAHRV
                     GKVKSERFFVDARVYPQTLDVMKTRAKYFGFELVVGDFAQADEGEYFGALFQYVGKDG
                     DVQDLQDVIGRLKAKGTIVAVAADIMSLVLLKSPAELGADIALGNTQRFGVPMGFGGP
                     HAAYFAFKDEFKRSAPGRIIGVSKDASGKPALRMALSTREQHIRREKATSNICTAQAL
                     LANLAGMYAVYHGPEGVKRIANRIHALASAFADALVSDGLNVVHEVFFDTVTVDFGSK
                     EKADQVFAVALESGYNLRRVSDTQIAAAFHETSVREDLTDLYRAFTGKDAFAFADDVK
                     GRLNAELLRQDDILQHPVFNRYHTEHEMLRYLKKLEDRDLAMNRSMISLGSCTMKLNA
                     TAEMLPITWAEFSDIHPYAPEAQTVGYRELLADMENSLKAITGFDAISFQPNSGAQGE
                     YSGMLAIRRYQEAQGEAQRNICLIPKSAHGTNPATAAMLGLKVVVVDTDEHGNVNIDD
                     LKAKAEQHRDALSAIMITYPSTHGVYEEGIRDICRIVHENGGQVYMDGANLNAQIGIM
                     QPAEVGADVLHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPFAPGHTLTDTHSASAGQT
                     AVAAAAYGSASILPITWMYLTMMGKQGMEQATRWALLNANYVAKRLSEDYPILYTGKN
                     GRVAHECIVDLRPLKAESGITETDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKA
                     ELDRFIAALKSIRREVQKVIDGEWPKDDNPLVNAPHTAADITGNWAHPYSREEAVFPL
                     PFVREHKFWPFVNRVDDVYGDRNLVCSCPPMENYED"
     gene            complement(3122..3481)
                     /locus_tag="ACFM8S_001275"
     CDS             complement(3122..3481)
                     /locus_tag="ACFM8S_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314342.1"
                     /translation="MTDRPSLLWIKPGQAEIMEYCEPEEASDPYATYRRANLMAGLPL
                     FVVILVLLNIVFPLPAYPLAWLVLAGFMVLVSGFPLSLLLVVLLVLTCCILARCPPLS
                     RLLCYPCPNHPMSKNSA"
     gene            3480..3659
                     /locus_tag="ACFM8S_001276"
     CDS             3480..3659
                     /locus_tag="ACFM8S_001276"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HFC6314343.1"
                     /translation="MPSEPAGRLFQKTPYRCNFMRLYGVGFALQTAYASRSSLVSQSV
                     HDYINNLGADKPLFI"
     gene            complement(3652..4293)
                     /locus_tag="ACFM8S_001277"
     CDS             complement(3652..4293)
                     /locus_tag="ACFM8S_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HFC6314344.1"
                     /translation="MPSEKPMNRRTFLLGAGALLLTACGRKSARTHAKIPEGSTVLAL
                     GDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPKLVIV
                     GIGGNDFLRKVPKEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLSDHPLYE
                     DLSEEYGIPLFGGAWAEILGDNNLKSDQIHANGKGYRKFAEDLNQFLRKQGFR"
     gene            complement(4283..4834)
                     /locus_tag="ACFM8S_001278"
     CDS             complement(4283..4834)
                     /locus_tag="ACFM8S_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFC6314345.1"
                     /translation="MNYCEFAASLPENTDNPNKHYHDTQYGFPIEDDNELFERLVLEI
                     NQAGLNWTLMLKKRQAFQTAFEGFDIDTVAAFGEADIERLLADAGIVRNRLKIDAAIF
                     NARQIQALQQEYGSFKNWLDAHHPRSKDEWVKLFKKHFKFVGGEIVGEFLMSTGYLKG
                     AHAESCPVYRETLKYHPKWLDAI"
     gene            complement(4838..5356)
                     /locus_tag="ACFM8S_001279"
     CDS             complement(4838..5356)
                     /locus_tag="ACFM8S_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFC6314346.1"
                     /translation="MRLFPIAAALTLAACGTVQSTQYFVLPDSRYIRPATQGGETAVE
                     VRLAEPLKRGGLVYQTDPYRLNTAQNHVWADTLDDMLEAALSNAFNRLDSTRIFVPAS
                     RRGSTEKWTVYIDAFQGSYTGKTLISGYAVLPDGTNRPFHIETEQQGDGYAAMTAALE
                     QGLKQAAQQMVE"
     gene            complement(5356..7017)
                     /gene="pqiB"
                     /locus_tag="ACFM8S_001280"
     CDS             complement(5356..7017)
                     /gene="pqiB"
                     /locus_tag="ACFM8S_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFC6314347.1"
                     /translation="MTDNSPPPNGHAQARVRKNNTFLSAVWLVPLIALIAGGWLWVKE
                     IRNRGPVVTLLMDSAEGIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVS
                     GLIRSDTQFWVVKPRIDQSGVTGLGTLLSGSYIAFTPGKSDEAKDVFQVQDIPPVTAI
                     GQSGLRLNLIGKNDRILNVNSPVLYENFMVGQVESAHFDPSDQSVHYTIFIQSPNDKL
                     IHSASRFWLESGINIETTGSGITLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTL
                     YDSRSEVANLPDDRSLYYTAFFKQSVRGLTVGSPVEYKGLNVGVVSDVPYFDRNDSLH
                     LFENGWIPVRIRIEPSRLEINADEQSKEHWKQQFQTALNKGLTATISSNNLLTGSKMI
                     ELNDQPSASPKLRPHTVYAGDTVIATQGGGLDDLQVKLADLLDKFDKLPLDKTVAELN
                     GSLAELKSTLKSANAALSSIDKLVGKPQTQNIPNELNQTLKELRQTLQGVSPQSPIYG
                     DVQNTLQSLDKTLKDVQPVINTLKEKPNALIFNSSSKDPIPKGSR"
     gene            complement(7010..8344)
                     /locus_tag="ACFM8S_001281"
     CDS             complement(7010..8344)
                     /locus_tag="ACFM8S_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiA/YebS family transporter subunit"
                     /protein_id="HFC6314348.1"
                     /translation="MPPLIFATFPMKPFAENIPHSLRGNCRDEALPPHTVDCPECGCR
                     ADVPQLDRGEAAFCPRCGYKLFRVGRHPFSAPPAYAAASLILMAFAYGMTYIEVGIPG
                     AASVLSLPEMMRLMVFQDYGFLAEVMFVLTFGAPVLFLLLCLYVYAALIRKQAYPALR
                     LATRVMVRLRQAMMVDVFFVSTLVAYIKLSSVAEVRFGSAFYLMFALSVMLIRTSVSV
                     PQHWVYFQIGRLTGDNAVQTASEGKTCCSRCLYFRDSAESPCGVCGAELYRRRPKSLS
                     ISSAFLTAAVILYFPANILPIMISSNPAATEVNTILNGIAYMWDEGDRLIAAVIFSAS
                     ILVPVLKIAAMSVLIASARFALPTGAKKLSHLYRITEAVGRWSMIDIFVIIILMCSFH
                     TYAARVIPGSAAVYFCLVVILTMLSAYYFDPRLLWDKRASDGIAFNETEKHD"
BASE COUNT         1933 a         2219 c         2462 g         2039 t
ORIGIN      
        1 tttgtccgtg tggaaatgac atcaaagggt gtgaataggc ttttcatctt tgaaatctgt
       61 ttgcacgatc ggatgaataa aaagtaggat gataaaaaag ctgatgtatg gatactggtt
      121 taactcattt gttttaaagt tatagtcgag atacgtgtat tggtgcaacc agtctgccgt
      181 cgacgaaaat gccgtctgaa agtgtttcag acggcatttg tcttcggggt ttcaacggtc
      241 agtcttcata attttccatc ggtgggcagc tgcacacgag attgcggtcg ccgtacacgt
      301 cgtccacgcg gttgacgaag ggccaaaact tgtgttcgcg gacgaacggc aacgggaaga
      361 cggcttcttc gcgggaatac ggatgcgccc agttgccggt tatatctgcg gcggtgtgcg
      421 gagcattcac cagcggatta tcgtctttcg gccattcgcc gtcgatgact ttctgcactt
      481 cgcggcgaat ggatttcagg gcggcgataa agcggtcgag ttcggctttg ctttcgctct
      541 cggtcggctc gatcatcagc gtaccggcaa cagggaagga aaccgtcggc gcgtggaagc
      601 cgtagtccat caggcgtttg gcgatgtcgg tttcggtaat gccactttcg gctttgagcg
      661 gacgcaaatc aacgatacat tcgtgcgcga cgcggccgtt tttgcctgta tacagaatcg
      721 gatagtcttc gctcagacgt ttggcgacgt agttggcgtt gagcaatgcc cagcgtgttg
      781 cctgctccat gccttgtttg cccatcatgg tcaggtacat ccaagtaatc ggcaggatgg
      841 atgcagaacc atatgctgcg gcggcaacgg cggtttgccc ggcacttgcg ctgtgtgtgt
      901 cggtcagggt atgccccggt gcgaacgggg cgaggtgggc tttcaagcca atcggaccca
      961 tgcccgggcc gccgccgccg tgagggatac agaaggtttt gtgcaggttc atgtgcaaca
     1021 cgtccgcgcc gacttcggca ggctgcatga taccgatttg agcgttgagg ttggcgccgt
     1081 ccatataaac ctgtccgccg ttttcgtgga cgatgcggca gatgtcgcgg atgccttctt
     1141 cgtacacgcc gtgggtggac ggataggtaa tcatgatggc ggacaaagcg tcgcggtgtt
     1201 gctcggcttt ggctttcaaa tcgtcgatgt tgacgttgcc gtgttcgtcg gtatcgacga
     1261 cgacgacttt caaaccgagc atggcggcgg tggcggggtt ggtgccgtgg gcggatttgg
     1321 gaatcaggca gatgttgcgc tgcgcttcgc cttgcgcttc ctgatagcgg cggatggcaa
     1381 gcataccgct gtattcgccc tgcgcgccgg agttgggctg gaaggaaatt gcgtcaaagc
     1441 cggtgatggc tttcaggctg ttttccatat cggcgagcaa ttcgcggtag cccacggttt
     1501 gcgcttcggg agcgtaggga tggatgtcgg agaactcggc ccaagtaatc ggcagcattt
     1561 ccgcagtcgc attgagcttc atggtgcagc taccgagcga aatcatgctg cggttcatcg
     1621 ccaagtcgcg gtcttcgagt tttttcaggt agcgcagcat ttcgtgttcg gtgtggtaac
     1681 ggttgaacac aggatgttgc agaatgtcgt cctgacgcag caattctgca ttcagacggc
     1741 ctttgacatc atcggcaaat gcgaacgcat ccttgccggt aaacgcgcgg tacaaatctg
     1801 tcaaatcttc gcgcacggat gtttcgtgga aggcggcggc gatttgggta tcgctgacgc
     1861 ggcgcaggtt gtaacccgat tccaaagcaa cggcaaacac ttggtctgct ttctctttgc
     1921 tgccgaaatc gacggtaacg gtatcgaaga agacttcgtg aaccacattc agaccgtctg
     1981 aaaccagcgc atcggcaaag gcagaagcca gcgcgtgaat gcggttggcg atgcgtttca
     2041 cgccttcagg gccgtggtaa acggcataca taccggccaa gttcgccagc aatgcctgcg
     2101 cggtacaaat attggatgtc gctttttcgc ggcggatgtg ttgctcgcgg gtggacaacg
     2161 ccatgcgcaa ggcaggtttg cccgatgcgt ctttggatac gccgatgatg cggcctggag
     2221 cggaacgttt gaactcgtct ttaaacgcga aataagcagc gtgcggacca ccgaagccca
     2281 tcggcacacc gaagcgttga gtgttgccca acgcaatgtc cgcgcccaat tcagccggag
     2341 acttcagcaa aaccaaactc atgatgtcgg cggcaacggc cacgatcgtg ccttttgctt
     2401 tcagacggcc gataacgtcc tgcaagtctt gcacatcgcc gtctttgccg acgtattgga
     2461 acagcgcgcc gaagtattcg ccttcgtcgg cttgggcaaa atcgccgacc accagttcaa
     2521 agccgaaata tttggcgcgg gttttcatca cgtccaaagt ttgcggatac acgcgcgcat
     2581 ccacaaagaa acgctcggat ttcaccttgc ccacgcggtg cgccatcgcc atcgcttcag
     2641 ccgcagcagt tgcctcgtcc agcaaagacg cgcccgccac agggaaaccg gtcaaatcga
     2701 tacacacttg ttggaagttt aacaacgctt ccaaacgacc ctgggcaatt tccgcctgat
     2761 acggcgtgta ggcggtgtac caacccggat tttccaacac gttgcgcaaa atcacgttcg
     2821 gcacgcgggt cgggtaatag cctaaaccga tataggactt gttgattacg tttttcgacg
     2881 caatgccttt caatttcgcc aatgcgtccg cctcggtcaa ggcctcgggc aaatcgagtt
     2941 cggacggcat acggatgctt tgcggcacgg tattgccgac aaaatcgtcc atactcttct
     3001 cgccgagcgt ttccaaaagc gcggcctcgt cgccgaaact caaatgttgc gcggcaaatt
     3061 cgttggggtt gaacagttcg gacagtttca tttctactcc ttcgggtttg tagggctgcc
     3121 atcaggcaga atttttagac atcggatgat tcgggcaagg gtagcacaaa agacgggata
     3181 atggcggaca acgcgccaga atgcagcagg tcaggacaag cagcaccaca agcagcagcg
     3241 ataagggaaa gccgctgacc aaaaccatga aacctgcaag caccagccaa gccaatggat
     3301 acgccggaag cggaaaaaca atattgagca gaaccaaaat cacgacaaac agcggcagac
     3361 ccgccatcag gttggcacgc ctgtatgttg cgtacgggtc ggatgcttct tcaggttcgc
     3421 aatattccat gatttcagcc tgtccgggct ttatccacag caggctcgga cggtcggtca
     3481 tgccgtctga accggcaggc cgtctttttc aaaaaacgcc ataccgctgc aacttcatgc
     3541 ggctgtatgg cgtaggcttt gcacttcaga cggcatatgc gtcccgaagc agtttggtgt
     3601 cccagtcggt tcatgattat ataaacaact tgggtgcgga taaacctttg tttatctaaa
     3661 cccctgtttt ctcaaaaatt gattcaaatc ttcggcaaat ttccgatagc ctttgccgtt
     3721 ggcgtggatt tggtcggatt tcagattatt atcgcccaaa atttccgccc acgcgccacc
     3781 gaacagcgga atgccgtatt cctcggacaa atcctcatac agcggatgat cgctcaaatg
     3841 cccgaacaac gcgcccagtg tgatgtgcgg cacgccgacg aggacggcgg ggatgttttc
     3901 cttctgcacg gtttcgatga ttttcgcgat attggcgcgg gtctgctcct tgggaacttt
     3961 gcgcagaaag tcgttgccgc ctatgccgac aatcacaagc ttgggtttgc gtgccaacag
     4021 cgcgggcagg cgcgacaggg cttgggcgga tgtatcgccc gatacgccgc cgttgacaat
     4081 attccaaccc gtcagttttt gcagttgcgc ggggtaggat tcgccggggt ttgcgccgta
     4141 gccgaaggta agcgaatcgc ccaaggcaag cacggtgctt ccttcgggaa ttttggcgtg
     4201 ggttcgggcg gattttctgc cgcaggcggt aaggagcaac gcgcctgcgc cgaggaggaa
     4261 ggttcttctg ttcattggtt tttcagatgg catcgagcca tttcgggtgg tatttcaggg
     4321 tttcacggta aaccggacag ctttcggcgt gcgcgccttt gaggtagccg gtactcatca
     4381 gaaattcgcc gacgatttcg ccgccgacga atttgaaatg ttttttaaag agtttaaccc
     4441 attcgtcttt gcttcgcgga tggtgcgcgt cgagccagtt cttgaacgag ccgtattctt
     4501 gttgcaacgc ttggatttgc cgtgcattga aaatggcggc atcgattttc aggcggttgc
     4561 ggacgatgcc agcgtcggca agcagccgct caatatcggc ttcgccaaat gcggcaaccg
     4621 tatcgatgtc gaaaccttca aatgccgtct gaaacgcctg ccgcttcttc agcatcagcg
     4681 tccagtttaa tcctgcctga ttgatttcca acaccagccg ctcaaacaat tcattgtcgt
     4741 cctcaatcgg aaaaccgtat tgcgtgtcgt ggtaatgttt gttcgggttg tcggtgtttt
     4801 cgggaagtga ggcggcaaat tcgcaatagt tcacggttta ctcgaccatc tgttgcgccg
     4861 cctgtttcag tccctgttcg agtgcggcgg tcatcgcggc gtagccgtca ccctgctgtt
     4921 cggtttcgat atggaagggt ctgttcgtac cgtcgggtag gacggcgtag ccgctgatga
     4981 gggttttgcc cgtgtagctg ccttggaatg cgtcaatata gaccgtccat ttttcggtac
     5041 tgcccctgcg tgaggcagga acaaagatgc gtgtgctgtc caaacggttg aatgcattgc
     5101 tcaacgccgc ttcgagcata tcgtccaagg tgtccgccca aacgtggttt tgtgcggtgt
     5161 tgaggcggta ggggtcggtt tgatagacca gtccgccgcg tttgagcggt tcggcaaggc
     5221 ggacttcgac ggcggtttcg ccgccttgcg ttgcagggcg gatgtagcgg ctgtcgggca
     5281 acacgaaata ttgtgtgctt tgcacagtac cgcaggcggc aagcgtcagg gcggcggcga
     5341 tggggaagag gcgcattatc ggcttccttt cgggatgggg tctttgctgc tgctgttgaa
     5401 aatcagcgcg ttgggttttt ctttcaaagt attaattacg ggttgaacgt cttttaaggt
     5461 tttgtccaaa ctttgcaacg tattttgtac gtcgccgtag ataggcgatt gaggcgatac
     5521 gccttgcagg gtttgacgta attctttcag ggtttggttc agttcgttcg gaatgttttg
     5581 tgtctgcggt ttgccgacca gtttgtcgat ggagcttagg gcggcattgg cagatttgag
     5641 tgtggatttg agctcggcaa gcgaaccgtt caattcggca accgtcttat ctaaaggcag
     5701 tttgtcgaac ttgtccagca aatccgccaa tttgacctgc aaatcgtcca aaccgccgcc
     5761 ctgggtcgcg ataacggtat cgcctgcata aacggtatgc ggtcgcagct taggcgatgc
     5821 ggaaggctga tcgttcaact caatcatttt gcttccggtc agcaggttgt tgctggagat
     5881 ggtggcggtc aggcctttgt ttaaggccgt ctgaaattgt tgtttccaat gttctttgct
     5941 ttgttcgtcg gcattgattt ccaaacggga aggttcaatg cggatgcgta cgggtatcca
     6001 gccgttttca aacaggtgca ggctgtcgtt gcggtcgaaa taagggacat cggaaaccac
     6061 gccgacattc agccctttgt actcgacggg cgaaccgacg gtcaggccgc gcacggattg
     6121 tttgaaaaac gcggtgtagt acagcgagcg gtcgtcaggc aggttggcga cttcgctgcg
     6181 gctgtcgtaa agcgtgaagc tgtcttcgct tttgacgttt ttactgtttt tggttttcgg
     6241 cgaatcaaat gaaatcgcgc ccgacagcag ggcaggcaga ggggcggaat tgagtgtgat
     6301 gccgctgcct gtggtttcga tattgatgcc gctttccagc caaaaacggc tggcggaatg
     6361 aatcagtttg tcgttggggc tttggatgaa gatggtgtaa tgcacgcttt ggtcggacgg
     6421 gtcgaaatgc gcgctttcga cttgcccgac cataaagttt tcatacaaaa cagggctgtt
     6481 gacgttgagg atgcggtcgt ttttaccaat caaattcaag cgcagcccgc tttgcccgat
     6541 ggcggtaacg ggcggaatgt cctgcacttg gaacacgtct tttgcctcgt cgcttttgcc
     6601 gggtgtaaag gcgatgtacg aacccgaaag cagcgtaccc aaaccggtta cgccgctttg
     6661 gtcgatacgc ggcttgacca cccaaaactg ggtatcgctg cggatgaggc cggatacgtc
     6721 cgcattgagt tgggcggtta cttccacgcc tttttggtcg tcgcgcagtt tgattcgggt
     6781 aacgcgtccg acatcgatgc tcaatacttt gatgaccgta ttgttgacct caatgccttc
     6841 cgcgctgtcc atcaagagcg taaccacagg tcccctgttg cggatttcct taacccaaag
     6901 ccagccgccg gcaatcagcg cgatcagcgg aaccagccag acggcagaga ggaaggtgtt
     6961 gtttttgcgg acgcgtgctt gggcgtgtcc gtttggagga gggctgttgt cagtcatgtt
     7021 tttccgtttc attgaaagca atgccgtctg aagcgcgttt gtcccaaagc aggcgcgggt
     7081 cgaaataata ggcggacagc atcgtcagaa tcacgaccag gcagaaatag actgccgcac
     7141 tgcccggaat gacgcgcgcg gcataagtgt ggaacgaaca catcaaaata ataatcacaa
     7201 aaatatcaat catcgaccag cggccgaccg cttcggtgat gcggtagagg tgcgacaatt
     7261 tctttgcgcc cgttggcaaa gcgaagcggg cggacgcaat caaaaccgac attgccgcaa
     7321 tcttcagtac cggcaccaaa atactcgcgc tgaaaataac cgccgcaatc agcctgtcgc
     7381 cctcgtccca catataagcg atgccgttaa ggatggtatt gacctccgtg gcggcaggat
     7441 tggacgaaat cataatcggc aggatattgg cagggaaata caaaataacc gccgccgtca
     7501 gaaacgccga cgaaatactc agacttttcg gccgccggcg gtacagttcc gcaccgcaca
     7561 cgccgcaggg ggattcggca ctgtcgcgga aatacaggca gcggctgcaa caggttttgc
     7621 cttctgatgc cgtctgaacc gcattatccc ccgtcagccg cccgatttga aaatacaccc
     7681 aatgctgggg aacggatacc gaagtccgaa tcagcataac cgacagcgcg aacatcagat
     7741 aaaacgccga cccgaagcga acctctgcca cagacgagag cttgatatac gccaccagag
     7801 tggaaacaaa aaacacatcc accatcatcg cctgcctcaa gcgcaccatt acgcgtgttg
     7861 ccaaacgcag cgcaggatac gcctgtttcc gtatcagcgc ggcatagaca tacaggcaca
     7921 gcagcagaaa cagaaccggc gcgccgaagg tcagcacaaa catcacttcg gctaaaaaac
     7981 cataatcctg aaacaccatc aggcgcatca tctcgggcag cgaaaggacg gatgccgcac
     8041 ccggtatccc gacctcgata tacgtcatac cgtaagcaaa cgccatcaaa atcagcgaag
     8101 ccgccgcata agcgggcggg gcggaaaaag gatgcctgcc caccctgaag agcttatagc
     8161 cgcagcgcgg gcagaacgcc gcctcccccc tgtccaattg cggcacatcc gcgcggcagc
     8221 cgcattccgg gcaatctacc gtatgcggcg gcagggcttc gtcgcggcag ttgccgcgaa
     8281 ggctgtgggg gatattttcg gcaaacggct tcatcgggaa agtggcaaat ataagtggcg
     8341 gcattataaa ggaaaaaagg cgatacaccc aatcaggatg caaaaccggc aggttcggca
     8401 gtatgaacaa acccttggtg tccggcaaaa atattttttg agcggttgtc cgccgactgc
     8461 cgaacccatt tgataaaatc ccgattctgt aaagaatagt aaaatacctg cttaaaaaca
     8521 tcacgcaaat gatatttttt ccaaaaatat gtgataataa atttcgttta tgattcttta
     8581 ttatttttat agtggattaa caaaaatcag gacaaggcgg cgaagccgca gacagtacaa
     8641 atagtacgga acc
//
LOCUS       DAXHSF010000080         8566 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.080, whole genome shotgun sequence.
ACCESSION   DAXHSF010000080 DAXHSF010000000
VERSION     DAXHSF010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8566
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.080"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            147..1331
                     /gene="coaBC"
                     /locus_tag="ACFM8S_001282"
     CDS             147..1331
                     /gene="coaBC"
                     /locus_tag="ACFM8S_001282"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFC6314349.1"
                     /translation="MGKHILLGVTGSIAAYKSCELVRLLKKQGHSVTVVMSRSATEFV
                     SPLTFQALSGNPVLTDTHGGNGSNGMEHINLTRNADVFLIAPASMNTVAKICNGVADN
                     LLTSLAAARKCPLAIAPAMNVEMWLNPANQRNIAQLVSDGITVYMPGLGEQACGENGM
                     GRMPEPAELLDLLPDLWTPKILRGKKILITAGATFEAIDPVRGITNTSSGKMGVALAR
                     ACRAAGAEVSLIHGQLQTALPFGISDTVQAVSAEDMHRAVHRLIEKQDAFISVAAVSD
                     YKVKNRSTQKFKKDKNAKRLSIELDENPDILASIASLPNPPFCIGFAAETENVMAYAR
                     EKRIKKKIPVIVANDVSIAMGKPTNQITIIGDDGELSFPETSKDEAAMQIVERLAVYL
                     NK"
     gene            complement(1398..3554)
                     /locus_tag="ACFM8S_001283"
     CDS             complement(1398..3554)
                     /locus_tag="ACFM8S_001283"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFC6314350.1"
                     /translation="MPAPQPSAPYDPLTAEARALLFHTASYLKPEEQAELEKAVAYAF
                     RAHDGQTRKSGEPYITHPIAVATQLALWHMDIQGLCAGVMHDVLEDTGVTKGEMAAVF
                     GNTIAEMVDGLSKLEKLKFEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTL
                     GSMRPDKRRRIARETLEIYAQIANRIGLNNAYRELQDLSFQNLHPNRYETLKKAMDKS
                     RKNRQDVVGKVLRAFGQRLVGANIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGF
                     RVIVNSIPACYAALGALHTLYQPKPGRFKDYIAIPKSNGYQSLHTTLVGPYGLPIEVQ
                     IRTREMDAVAEGGIAGHWSYKSDSKTVDQAVLHTNRWLKNILDLQASSANAIEFLEHV
                     KVDLFPNEIYILTPKGKILTLPKGATPIDFAYAVHTDIGHKTVAARINNIMMPLRTKL
                     KTGDSVEIITSEHAKPNPAWLNFAVSGRARSAIRQYIKNLNRHDAVVLGESLLQKALS
                     SLLPKDVLLSDGIKEKYLADLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAELAGEHFG
                     SEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRLLLVKGKGMIIHRDTCPTLLKSDSE
                     QQLDADWENMNGQNYRVGLQVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTE
                     GFVEFKFLLKVKNLNQLNQIIQNLHSIPYIRKVIRS"
     gene            complement(3646..3852)
                     /gene="rpoZ"
                     /locus_tag="ACFM8S_001284"
     CDS             complement(3646..3852)
                     /gene="rpoZ"
                     /locus_tag="ACFM8S_001284"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFC6314351.1"
                     /translation="MARITTEDCTGKISNHFDLTLVAARRARQLENGNTPLVDDVRNN
                     KPTVTALREIAAGHIGTELLTRNK"
     gene            complement(3911..4528)
                     /gene="gmk"
                     /locus_tag="ACFM8S_001285"
     CDS             complement(3911..4528)
                     /gene="gmk"
                     /locus_tag="ACFM8S_001285"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFC6314352.1"
                     /translation="MSAYRKGNIFIISAASGTGKTTLVSRLLANHNGLRVSVSHTTRP
                     PREGEANGVHYHFVSKEEFESLIEQEAFLEYADVFGNYYGTSAEGVNALAAAGYDVIL
                     EIDVQGAAQVRDALPEAVGIFILPPSFDVLAARLNGRGTDSQEVIQRRLSKARHEIEQ
                     SVLFDFVVVNDDLARAEEDLLHIVNACRLKRSRQLGFIADLLENS"
     gene            4656..5255
                     /locus_tag="ACFM8S_001286"
     CDS             4656..5255
                     /locus_tag="ACFM8S_001286"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFC6314353.1"
                     /translation="MALKTSNLEHAMLVHPEAMSVGALADKIRKIENWPKKGILFHDI
                     TPVLQSAEYFRLLVDLLVYRYMDQKIDIVAGLDARGFIIGAALAYHLNVGFVPIRKKG
                     KLPFETVSQSYALEYGEAAVEIHTDAVKPGSRVLLVDDLVATGGTMLAGLELIRKLGG
                     EIVEAAAILEFTDLQGGKNIRASGAPLFTLLQNEGCMKG"
     gene            complement(5301..6089)
                     /locus_tag="ACFM8S_001287"
     CDS             complement(5301..6089)
                     /locus_tag="ACFM8S_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFC6314354.1"
                     /translation="MNPKIVFFDIDDTLYRKYTDTLRPSVKTAVAALRGKGILTALAT
                     GRSLATIPEKVRDMMAETGMDAVVTINGQFALLHGKTVREVPMDAGLMGRVCAHLDGL
                     GMDYAFVGGEGIAVSALSECVCRALQHIASDFFADKDYFSSKPVYQMLVFAEENEMPP
                     WSDIVEREGLKTVRWHEEAVDLLPAGMSKTDGIRSVVEALGLEMADVMAFGDGLNDVE
                     MLSEVGFGVAMGNGEQAVKEAAKYVCPGVDEDGVLRGLQDLGVI"
     gene            6414..7769
                     /gene="yegQ"
                     /locus_tag="ACFM8S_001288"
     CDS             6414..7769
                     /gene="yegQ"
                     /locus_tag="ACFM8S_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFC6314355.1"
                     /translation="MKAPELLLPAGGLERMRAAYDYGADAVYAGSPRYSLRARNNEFA
                     KLNVLEQGIKEAHERNKKFFLTVNTLPHNSKLKTFVSDMEPLIAMKPDALIMADPGLI
                     MTVREKWPEMPIHLSVQANTTNYWGVKFWQNIGVERIILSRELSMEEIAEIRQECPDI
                     ELEVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVHNATESDAGDAQLL
                     QGFNFEKAQEEANQNFEGINGQKRHPYADKVFLIEESNRPGEMMPIMEDEHGTYIMNS
                     KDLRGIEVVEKLAKIGVDSLKVEGRTKSLYYVARVAQSYRKAIDDAVAGRPFDYSLLS
                     ELEGLANRGYTSGFLERHHTQDYQNYLTGHSTAKQSQYVGHVTEIDENGWATVEVKNR
                     FAVGDSLEIIHPNGNQTIKLEQMTRKGQPVDVAPGNGIQVKIPDMQGKEKALIARVLN
                     P"
     gene            7903..8379
                     /locus_tag="ACFM8S_001289"
     CDS             7903..8379
                     /locus_tag="ACFM8S_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
                     /protein_id="HFC6314356.1"
                     /translation="MLAACQSGSAPKNAHTASADTPAGESCKVIRDDTAGSGKNIIYR
                     CCNGQAAINAAIADGVLESGIPVSFGGGGVPIANGRNLVTRQAANSVGKSDSEACEHA
                     LINAARKFQQTAGKLGGRSVTGFHSYFDKQPLQGGQYDCQAGSFHVRVVMRANVVR"
BASE COUNT         2166 a         2342 c         2039 g         2019 t
ORIGIN      
        1 ccctcctctg accggtgcgc ctttgtgaat atgccgtctg aaactcgggg actcagacgg
       61 cattttgtat ccaaacggta tctaatgtat ccacactttg ttatagaatg gctgctgttt
      121 tttcttcgta attagaaatt gtcaaaatgg gcaaacatat tcttttaggt gtaacgggca
      181 gtattgcggc gtataagtct tgcgagttgg ttcgtctgct gaaaaaacag gggcattcgg
      241 ttacggtggt tatgagccgc tcggcgaccg agtttgtctc ccctttaact tttcaggctt
      301 tgagcggcaa tcctgtcctg accgacacgc atggcggcaa cggttcaaac ggtatggaac
      361 atatcaacct gacccggaat gcggatgttt ttctgattgc gccggcaagt atgaataccg
      421 tggcaaaaat ctgtaacggc gtggcagata acctactgac cagtctggca gccgcacgga
      481 aatgtccgct tgccatcgct cccgcgatga atgtggaaat gtggctcaac cctgccaacc
      541 aacggaatat cgcacaactg gtttcagacg gcattactgt ctatatgcca ggcttgggcg
      601 aacaggcttg cggagaaaat ggtatgggaa ggatgccgga acctgccgaa ttgctggatt
      661 tgcttccgga cttgtggaca ccgaaaattt taaggggcaa gaaaatcttg attaccgcag
      721 gtgcgacatt tgaagccatt gaccctgtcc gaggcatcac aaatacctcc agcggaaaaa
      781 tgggcgtggc tctggcgcgg gcgtgccgtg ccgccggtgc ggaagtcagc ctgattcacg
      841 gacagcttca aaccgcgctg cctttcggca tatccgatac ggttcaagcc gtcagtgccg
      901 aagatatgca tcgcgcagtg catcgtttga ttgaaaaaca agatgctttt atttctgttg
      961 ccgccgtctc agactataag gttaaaaaca ggagtacgca aaaattcaaa aaagataaaa
     1021 atgccaaacg gctatccatc gaattggatg agaaccccga tattttggct tctattgcct
     1081 cattgccgaa cccgccgttc tgcatcggtt ttgccgctga aacggagaat gtaatggcat
     1141 atgcacgaga aaaacgtatt aagaaaaaaa taccggtaat agttgccaat gatgtttcaa
     1201 tcgcgatggg caaaccgacc aaccagatta ccattatcgg ggacgacggg gaactatctt
     1261 ttcccgaaac aagtaaagat gaagcggcga tgcagattgt tgaaaggctt gccgtatatt
     1321 tgaacaaata agcaattgaa cggataaacc ataaagcggg ttgcctgtta atcaaaaggc
     1381 aacccgcttt acctgcttca acttctgatg actttgcgga tatatggaat actatgcaga
     1441 ttttgaataa tctgattcaa ttgattcaga ttcttgactt tcaataagaa tttgaattcg
     1501 acaaaacctt ccgttcccga ctgggattta gacggtgttt cgaccgactc aatgtccgca
     1561 ccggaatcgg aaatcgcttg cgccattaat gccaacaggc cgtggctgtc ttccgattgg
     1621 acttgaagcc cgacacggta gttctgccca ttcatatttt cccagtctgc atccagctgc
     1681 tgttcggaat cggacttcaa caacgtcggg caggtatccc tatggataat catgcctttt
     1741 cccttaacca acagcaaacg gatggaatca ccaggaacag gatggcagca ctctgcaaaa
     1801 tgaatatgcc cgctttcctg accatcgact ttaatggaac tgagcctgac ctcgctgccg
     1861 aaatgctccc ctgccaactc ggcaatgtgc atggcgacat aaacaggcag ggtatgcccc
     1921 ataccgacgt tgtacagcac ttcctcaaat gaagtctgtt tgtcgttgag atcggcaagg
     1981 tatttttctt tgatgccgtc tgaaagcagg acatctttgg gcagcaaact cgacagggct
     2041 ttttgtaaga ggctctctcc caaaacgacc gcatcgtgcc ggttaaggtt tttaatatat
     2101 tggcgtatgg cactgcgcgc cctgccggac acggcgaaat tcaaccatgc aggatttggt
     2161 ttggcatgtt cggatgtaat aatttcgacg gaatcgccgg ttttgagctt cgtacgcaac
     2221 ggcatcatga tattgttgat acgtgcggca acggttttgt gcccaatatc agtatgcacc
     2281 gcataagcaa aatcgatagg tgttgcaccc ttgggcaatg ttaggatttt tccttttggc
     2341 gtaaggatat agatttcgtt tggaaacaaa tctaccttga cgtgttcaag aaactcaata
     2401 gcattggcac tgctggcctg caaatctaag atatttttca gccaccggtt tgtgtgaagc
     2461 accgcctgat cgaccgtctt agaatctgat ttatagctcc aatgtccggc gataccgcct
     2521 tcggcaacag catccatttc ccgcgtgcgt atctgaactt caatcggcaa gccgtaaggg
     2581 ccgaccaaag tcgtatgcag actttgataa ccgttgcttt tcggaatagc gatgtagtct
     2641 ttgaaccgcc cgggcttggg ctgataaagg gtgtgcaatg cgccgagtgc ggcataacag
     2701 gcgggaatgc tgttgacaat gacgcggaaa ccgtaaatat ccataacctc ggcaaagcgc
     2761 agctttttcg ccatcatttt ctgatggatg ccgtacaggt ttttttccct gcctttgatt
     2821 ttggcctcta tattcgcgcc taccagcctc tgaccgaatg cgcgcaagac tttgccgaca
     2881 acgtcctgcc ggttcttccg gctcttgtcc attgcttttt ttaaagtctc gtaacggttg
     2941 ggatgaaggt tttggaacga taaatcctga agctcccgat acgcattatt caaacctata
     3001 cggttggcaa tttgcgcgta gatttcaagg gtttcccttg caatccggcg gcgtttgtcc
     3061 gggcgcatcg aaccgagtgt ccgcatatta tgcagacggt cggcaagttt aacgacaatc
     3121 acgcgtacat ctttggtcat tgccaaaatc agtttgcgga aactctccgc ctgatgctct
     3181 gcatgatctt caaatttgag tttttcaagc ttggacagac cgtccaccat ctcggcaatc
     3241 gtattgccga acaccgccgc catttcccct tttgtcacgc ccgtatcttc caatacgtcg
     3301 tgcatcacgc ctgcacaaag accctgtatg tccatatgcc aaagggcgag ctgcgtcgca
     3361 acggcaatcg gatgcgtgat gtagggctcc ccgcttttgc gggtttgccc gtcgtgggcg
     3421 cgaaacgcat aggcgacagc tttttcaagc tccgcctgtt cctcgggctt gaggtaggag
     3481 gcggtatgga aaagcagggc acgcgcttcg gcggtcaggg ggtcgtaagg ggcggaaggt
     3541 tggggggcgg gcatttcaga cggctttcgg tatgtatgtg tttttcattt caaaccgtcg
     3601 gactgcacgg cggcaaagtg ttccggcgtg cgtttccggc agaatttatt tattgcgcgt
     3661 caacagttct gtaccgatat gtccggcggc gatttccctt aaggcggtaa cggtcggttt
     3721 gttattgcgg acatcgtcca caagcggcgt gttgccgttc tcaagctggc gggcgcggcg
     3781 ggccgctacc aatgtcaggt caaaatggtt ggaaattttt ccggtacagt cttcggtggt
     3841 aatacgtgcc atattatttg ctttctttca aaaatattta aattgggaaa ccgggtattt
     3901 tcgcggtttt ttaggaattt tccaacaaat ctgcaataaa tcccagttgc cgcgaccttt
     3961 tcagacggca ggcattcaca atatggagca aatcctcctc cgcccgcgcc aagtcgtcat
     4021 tgaccacgac aaagtcgaac aatacagact gttcgatttc atgccttgcc ttcgacagcc
     4081 tcctttggat aacttcctga ctgtccgtcc cgcgtccgtt gaggcgcgcg gcaagcacat
     4141 cgaaagaagg cggcaggata aagatgccga cggcttcggg cagcgcatct cgaacctgag
     4201 ccgcgccctg aacgtcgatt tccaaaatca cgtcataacc tgccgccgcc aacgcattca
     4261 caccctcggc gcttgtgccg taatagttgc cgaacacgtc ggcgtattcc aaaaaagcct
     4321 cctgctcgat aagcgactca aactcttctt tggagacaaa gtgataatgt acgccgtttg
     4381 cttcgccttc acgcggcggg cgcgtcgtgt gcgacacgga aacgcgcaaa ccgttatggt
     4441 ttgccaacag ccgcgacacc agcgtggttt tgcccgtgcc ggaagcggcc gaaatgataa
     4501 agatgttgcc ttttcgataa gcggacatat tttttacctg tatattttcc agccgattgt
     4561 atcacaacgg acacccagtt tcctatttgc cgatgcccat attttgccgc tattgttttg
     4621 attgatttgg caagcggcag gctgacggct acaatatggc gttaaaaaca tcaaacttgg
     4681 aacacgcaat gctggttcat cccgaagcta tgagtgtcgg cgcgcttgcc gacaaaatcc
     4741 gcaaaatcga aaactggccg aaaaaaggca tcttattcca cgacatcacg cccgtcctgc
     4801 aaagcgcgga atacttccga cttttggttg atttattggt ttaccgctat atggatcaga
     4861 aaatcgacat cgttgccggt ttggacgcgc gcggcttcat tatcggcgcg gcactcgcct
     4921 accatctcaa cgtcggtttt gtccccatcc gcaaaaaagg caagctgcct tttgaaaccg
     4981 tatcgcaaag ctacgcgctc gaatacgggg aagctgcggt ggaaatccac accgatgccg
     5041 tcaaacccgg ttcgcgcgtg ctgctggtcg atgatttggt tgccacgggc ggcacaatgc
     5101 ttgccgggct ggaactgatc cgcaaactcg gcggagaaat tgtcgaagcc gccgccattt
     5161 tggaatttac cgaccttcaa ggcggcaaga atatccgtgc aagcggcgcg cccttattta
     5221 ccctgcttca aaacgaaggc tgtatgaagg gctgaaaacc gaccctgccg tctgaaaccg
     5281 gcagggttgt tattatgcat tcaaatcacg cccaaatctt gcaagcccct caacacgccg
     5341 tcttcatcaa cgccggggca aacatatttc gccgcctctt tcactgcctg ttccccgtta
     5401 cccattgcca cgccgaaccc gacttctgac agcatttcca catcgttcaa accgtcgccg
     5461 aacgccatca cgtccgccat ttccaaaccc aatgcttcaa ccacgcttct gatgccgtct
     5521 gttttcgaca ttcccgcagg cagcagatcg accgcttcct cgtgccagcg caccgttttc
     5581 aagccttccc gttccacaat atccgaccag ggcggcattt cgttttcctc cgcaaacacc
     5641 agcatctgat acaccggttt gcttgaaaaa taatccttat cggcaaaaaa atcgctggcg
     5701 atatgctgca aggcgcggca cacgcattcc gacagcgcgg acacggcgat cccctctccg
     5761 ccgacaaacg cataatccat acccaagcca tccaaatgcg cgcaaaccct gcccatcaaa
     5821 ccggcatcca tcggtacttc gcgcacagtt tttccgtgca gcagcgcaaa ctgtccgttt
     5881 atcgttacca cggcatccat gcccgtttcc gccatcatgt ccctgacctt ttcgggaatc
     5941 gtcgccaaag accgccccgt tgccaacgcc gtcaatatgc ctttgccgcg caaagccgcc
     6001 accgccgttt tcacggaagg acgcaaggta tccgtatatt tgcggtacag cgtatcgtca
     6061 atatcgaaaa acacgatttt aggattcatc acattctctc tcgcattcaa actgccgcat
     6121 tgtatcccaa tcaggcaaat acttgataaa tccttataaa tttcccggca aaattgaccg
     6181 cgaacacgaa aaggcggata atccgcccat cctcaaaccc ttttcagacg gcatttgcag
     6241 caatgccgtc tgaaacattt ttacaaagca tacaaatcat gtttcaacac acaggacgac
     6301 acataaagcg tcgccctata tgttgccctg attcggaaag ggttacgtcc ctcccaaata
     6361 aagtctgatt ctaccgccct aaagggcggg gtttcaaccg aaaaggaaac acgatgaaag
     6421 cacccgaact cttattgcca gcaggcggtc tcgaacgtat gcgcgccgcc tacgattacg
     6481 gcgcagatgc cgtttatgcc ggcagcccgc gttactccct gcgcgcccgc aacaacgaat
     6541 ttgccaaact caacgtcctt gagcaaggca ttaaagaagc gcacgagcgc aacaaaaaat
     6601 tcttccttac cgtcaacacc ctgccgcaca actccaaact caaaaccttt gtttccgata
     6661 tggagccgct gattgcgatg aagcccgacg cgctgattat ggcggatccg ggtttgatca
     6721 tgaccgtgcg cgaaaaatgg ccggagatgc cgattcacct atccgtacag gcaaacacca
     6781 ccaactactg gggcgtgaaa ttctggcaga acatcggtgt cgaacgcatt attctgtcgc
     6841 gcgaattgag catggaagaa atcgctgaaa tccgccaaga atgccccgat atcgaactcg
     6901 aagtcttcat tcacggcgca ttgtgcatcg cctattcagg ccgctgtctg ctctccggct
     6961 acttcaacca ccgcgacccc aaccaaggca cctgcaccaa ctcctgccgt tgggactaca
     7021 aagtccacaa cgctacggaa agcgatgcgg gcgatgccca gcttctgcaa ggtttcaact
     7081 ttgaaaaagc ccaagaagaa gccaaccaaa actttgaagg catcaacggt caaaaacgcc
     7141 atccctacgc cgacaaggtt ttcctgattg aagaatccaa ccgtccgggc gagatgatgc
     7201 cgattatgga agacgaacac ggcacctaca tcatgaactc caaagacctt cgtggcattg
     7261 aagtggttga gaaactcgcc aaaatcggcg tggacagcct caaagtcgaa ggccgcacca
     7321 agtcgctcta ttatgtcgca cgcgtcgccc aatcctaccg caaagcgatt gacgatgccg
     7381 tcgcaggccg tccgtttgat tacagcctgt tgagcgaact cgaaggcctt gccaaccgcg
     7441 gctacaccag cggcttcctc gaacgccacc acactcagga ttatcaaaac tacctgaccg
     7501 gccattccac tgccaaacaa agccaatacg tcggacacgt taccgaaatc gatgaaaacg
     7561 gctgggcaac agtggaagtc aaaaaccgct ttgccgtcgg cgattcgctc gaaatcatcc
     7621 acccgaacgg caaccaaacc atcaaattgg aacaaatgac ccgcaaaggc caacctgtcg
     7681 atgtcgcacc cggcaacggc attcaggtca aaatccccga tatgcagggc aaagaaaaag
     7741 ccctcatcgc acgcgtattg aacccctgag ccacaatgcc gtccgaaccc cgttttcaga
     7801 cggcattttg ttgaatctta cggtacaatg gcaatgattt cccacagcaa acaaggacat
     7861 ccgacatgaa aaaaaatcct catactctcc gccgccgccc tgatgctggc agcctgccaa
     7921 tccggcagcg cacctaaaaa cgcgcacacg gcatccgccg acacaccggc cggagaatca
     7981 tgcaaagtca tccgggacga caccgcagga agcggcaaaa acatcattta ccgttgctgc
     8041 aacggtcagg cggcaataaa tgcagccatt gcagacggcg tattggaatc agggattccc
     8101 gtcagcttcg gcggcggggg cgtaccgatt gcaaacggca ggaatctggt tacacggcaa
     8161 gcagcaaaca gcgttggcaa atccgactcc gaggcctgcg aacacgccct aatcaatgcc
     8221 gcccgcaaat tccagcagac cgccggcaaa ctcggcgggc gcagtgttac cggcttccac
     8281 agctacttcg acaagcaacc gctgcaagga ggacaatacg actgtcaggc aggctcgttc
     8341 cacgtccgcg tggtcatgcg cgccaatgtc gtccgttaac atatcggcga ccaaaaaatt
     8401 aggctgtgtt ttagtaatct gttgatttca attatttgca aaggaaaaga caattatttt
     8461 ttcggttagg aagagaccta tcctattgaa tacatttaag ttaaatacgt ctatcaacac
     8521 tatattatag tggattaaca aaaatcagga caaggcgacg aagtcg
//
LOCUS       DAXHSF010000081         8541 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.081, whole genome shotgun sequence.
ACCESSION   DAXHSF010000081 DAXHSF010000000
VERSION     DAXHSF010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8541
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.081"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            847..2055
                     /locus_tag="ACFM8S_001290"
     CDS             847..2055
                     /locus_tag="ACFM8S_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFC6314357.1"
                     /translation="MSEAEYFSHFISNGNGKLLEIPQRRGRQDGVFIDWLSFTLHEDS
                     LLKVSGCPLVSDAEYMFVLSKKLEEILGFGITSRCKSKGNKFYDSMFRLGSEEVDYGE
                     VHYGGQRNTVLIELKGVGCNIANPGWELRLKQFLEDSLRPRITRVDLALDFFDGEYTP
                     EQALLDHDNGFFDNSNMRPKSEMVGTAWRREDGSGKTFYVGRKKNSRFVRVYEKGRQL
                     GDKESKWVRFEIQFNYGDIEIPLDILINQGSYFSGAFPICQKFKNMPNPERFDYRKKV
                     ANLTFQHKLRYAKNAVGKLINFMFDMGFDSDEIVRYLKADLGYPKGLEPEKYSLAGLK
                     ESLKFGFIHEQPDVDLEVELEELGIIKFKQSDKFDPDKRLFDPHHDVESERQYQLYLD
                     RMYDLHANQN"
     gene            2076..2387
                     /locus_tag="ACFM8S_001291"
     CDS             2076..2387
                     /locus_tag="ACFM8S_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314358.1"
                     /translation="MFNQTQTVTYPATFLGAKKFKGEIDGSNIDTCSVLVATPLPAQS
                     GNAVGFTAAQMKFGDSKNFSKLENLKYPCGVMVTVEMTSTGKGMVPSLIDFQVAEKPK
                     G"
     gene            2391..2591
                     /locus_tag="ACFM8S_001292"
     CDS             2391..2591
                     /locus_tag="ACFM8S_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314359.1"
                     /translation="MKFEERFIVQDLETHDFIYPDPFGDVGFTQNIKSAGQFESYEDA
                     LNSGINEIGGGFQIFQFFVKSE"
     gene            2829..3059
                     /locus_tag="ACFM8S_001293"
     CDS             2829..3059
                     /locus_tag="ACFM8S_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314360.1"
                     /translation="MNIVKKYAVKALLAAGIFTPAIVMADGFDASAIGTQVANVIMGF
                     VAMVSAVGMAAITVILAIQGFKMAWSMIKSVK"
     gene            3088..3387
                     /locus_tag="ACFM8S_001294"
     CDS             3088..3387
                     /locus_tag="ACFM8S_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314361.1"
                     /translation="MGFRVGMNCFDTRLQADDYLLSSLPPTVTQDGKIIRPERVGDKW
                     ILNGKPVTLSYPKCSNYEQVKSGAYLGSMVLILFVVIYGFRLLINFLKDIGKVGA"
     gene            3506..3826
                     /locus_tag="ACFM8S_001295"
     CDS             3506..3826
                     /locus_tag="ACFM8S_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HFC6314362.1"
                     /translation="MNKPFITQAQLALYKYQPSSKYFGQSMALIASKEFEEFVRNVKE
                     YDVIECFSYFLNKRVTHNIWKIYFSDESNIFIRKSEENGKISHEFIYSEFSDSNTDFN
                     VLFS"
     gene            3810..5324
                     /locus_tag="ACFM8S_001296"
     CDS             3810..5324
                     /locus_tag="ACFM8S_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
                     /protein_id="HFC6314363.1"
                     /translation="MCCSLSFAEPARIDDRIIKFRPSKSKFFESTGYRKINNEFSKFT
                     EAANVEHIPTGAKARINAKITASVSRAAVLSGVGKLVRQGAKFSTRAVPYVGTALLAH
                     DVYETFKEDIQARGYQYDTETDKFVKGYEYSNCLWYEDERRINRTYGCYGVDSSIMRL
                     MSDDSRFPEVKELMESQMYRLARPFWNWHKEELNKLSSLDWNNFVLNRCTFDWNGGDC
                     VVNKGDDYRNGANFSLSRNPKYKEEMDAKKLEEILSLKVDANPDKYIKATGYPGYSEK
                     VEVAPGTKVNMGPVTDRNGNPVQVVATFGRDSQGNTTVDVQVIPRSDLTPGSAEAPNA
                     QPLPEVSPAENPANNPNPNENPGTSPNPEPDPDLNPDANPDTDGQPGTRPDSPAVPGR
                     TNGRDGEDGGLLCKFFPDILACDRLPEPNPAEDLNLPSETVNVEFQKSGIFQDSAQCP
                     APVTFTVTVLDSSRQFAFSFENACTIAERLRYMLLALAWAVAAFFCIRTVSREV"
     gene            5628..5912
                     /locus_tag="ACFM8S_001297"
     CDS             5628..5912
                     /locus_tag="ACFM8S_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HFC6314364.1"
                     /translation="MKLLAALIPLLMSVAGRILTALGLMAVTYSGVDRLVAHFQQAIT
                     SSITGAPQAMLQLFYISGGGTVLNILFGAIAFILSFKQMTKLATSIGKKK"
     gene            5913..7100
                     /locus_tag="ACFM8S_001298"
     CDS             5913..7100
                     /locus_tag="ACFM8S_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin domain-containing
                     protein"
                     /protein_id="HFC6314365.1"
                     /translation="MAEICLITGTPGSGKTLKMVSMMANDEMFKPDENGIRRKVFTNI
                     KGLKIPHTYIETDAKKLPKSTDEQLSAHDMYEWIKKPENIGSIVIVDEAQDVWPARSA
                     GSKIPENVQWLNTHRHQGIDIFVLTQGPKLLDQNLRTLVRKHYHIASNKMGMRTLLEW
                     KICADDPVKMASSAFSSIYTLDKKVYDLYESAEVHTVNKVKRSKWFYTLPVIILLIPV
                     FVGLSYKMLSSYGKKQEEPAAQESAATEHQAVFQDKTEGEPVNNGNLTADMFVPTLSE
                     KPESKPIYNGVRQVRTFEYIAGCVEGGRTGCTCYSHQGTALKEITKEMCKDYARNGLP
                     FNPYKEESQGRDVQQSEQHHSDRPQVATLGGKPWQNLMYDNWQERGKPFEGIGGGVVG
                     SAN"
     gene            7315..8271
                     /locus_tag="ACFM8S_001299"
     CDS             7315..8271
                     /locus_tag="ACFM8S_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISNgo3 family transposase"
                     /protein_id="HFC6314366.1"
                     /translation="MNIIGLDISKDTIDATLHKTNGSIHYIKFKNNDDGLKQFRLWIK
                     GNRIRKVYIGMEATGIYYEKAADMLSSYYTVYVINPLKIKDYGKSRFNRTKTDKADSN
                     LIADYIKRHQDTLIPYQIPKNKALQKLINLKNQLQQQQKQIKNRLHSTEEDFIRNIHQ
                     DLIDTIQDKMEQVKIAISEQIKKQTDYNHYRNLQTIPSIGKDTASVLYAQLTEKHFKT
                     ANQFVSYAGLSPAIIQSGTSVRGRGRLSRYGNRRLKSTLYMPALCAYRFNAFPKLINN
                     LKKAGKPKMVIIVAIMRKLAKLAYYIVKTGQPYDAERHRLNQ"
BASE COUNT         2654 a         1718 c         1993 g         2176 t
ORIGIN      
        1 aagtgggaat ctagaatctc agggtttcag tcatttccga tagattcccg ccgcgtcagg
       61 gggctggatt cccgcctgcg cgggaatgac gggtttcgag attgcggtgt tgtcggaacg
      121 caactgaacc gtcattccca cgaaagtggg aatctagaat ctcgggggtt cagtcatttc
      181 cgatagattc ccgccgcgtc agggggtcta gattcccgcc tgcgcgggaa tgatgggttt
      241 caagattgcg gtattgtcgg gaatgacgaa tccatccata cggaaacctg caccacgtca
      301 ttcccacgga agtgggaatc tagaatctcg gggtttcagt catttccgat agattcccgc
      361 cgcgtcgggg gtctagattc ccgccttata tgatgcgctc ttatatgatg cgctctatca
      421 aaggggcgca ttacttttct taacattccc ctttgacagc caagtgaaag gggctttttt
      481 atgtcagtag caaatgtaat attttcttgt tcctattgga gaatatttaa aaaatcagat
      541 tattgcgttt tatgtttcta tcagttcagg catggtgaac ccaataaact cgctgaacaa
      601 gaaaattttt caaagcttta tcaggcgttc gattatatag attcggttgg ctcgaatttt
      661 ccggtaatta tcacaacaga cggttgtggt ctttcttctt gatctttaac agtttgtcag
      721 gattgggctt tcggtcgttg accgttggac gcgctttagc gcggcagacg ggaaacggct
      781 gaaagccccc ccctgactaa caggggggga gcgaaataaa acaaatccct aaaggtactg
      841 aacaaaatga gtgaagcaga atatttttcc catttcatat ctaacggcaa tgggaagtta
      901 ttagaaattc cgcaacgtag gggcaggcag gacggcgttt ttatagattg gttgtcattc
      961 acactgcacg aagattcctt gctgaaagtt tccggatgcc ccttagtttc cgatgccgaa
     1021 tatatgtttg ttttaagcaa aaaattggag gaaatattag ggtttggcat cacgagcaga
     1081 tgcaaatcga agggcaataa attttacgat tcgatgttta ggttgggatc ggaagaagtt
     1141 gactacggcg aagtccatta cggaggtcag cgaaatacgg ttttaatcga attgaaaggt
     1201 gtaggttgca acattgcaaa tccaggttgg gaattgaggc ttaagcagtt tttggaagat
     1261 tcattgaggc cgaggataac gcgggtagat ttggcacttg atttttttga tggggagtac
     1321 acgccggaac aggcactttt ggatcacgat aacggttttt tcgataacag taacatgagg
     1381 ccgaaatctg aaatggttgg aacggcttgg cggagagagg acgggagcgg caagacattt
     1441 tatgtaggtc gcaagaaaaa ttctcgtttt gtgcgtgttt atgagaaagg caggcagcta
     1501 ggcgataaag aaagcaagtg ggtaaggttc gaaattcagt ttaattatgg agatatagaa
     1561 atacccttgg atattttaat aaatcaaggt tcttactttt caggcgcttt cccgatttgt
     1621 cagaaattta aaaatatgcc gaatccggaa aggttcgatt accgtaaaaa agtggctaat
     1681 ttaacttttc agcataaatt gagatacgca aaaaacgcgg tcggcaaact gattaatttc
     1741 atgtttgata tgggttttga tagtgatgaa attgtcagat atctgaaggc agatttgggg
     1801 tatcccaaag ggctagaacc tgaaaaatat tcgttggccg gattgaagga atctttgaaa
     1861 ttcggcttta tccacgaaca accggatgta gatttagagg ttgaattgga agaactcgga
     1921 attatcaaat ttaagcaatc agataaattc gatccggata aaaggctttt cgatccacat
     1981 cacgatgtag aaagtgagag gcaatatcag ctttatctcg acagaatgta tgatcttcat
     2041 gcaaatcaaa attaacctaa aaaggaaaaa ttaatatgtt taatcaaact caaactgtaa
     2101 cttatcccgc aactttttta ggagctaaaa aattcaaagg cgaaattgat ggctctaata
     2161 tcgacacttg ttccgtattg gttgcaacac ctttgccggc acagtcggga aatgctgttg
     2221 gattcacggc agcacaaatg aagttcgggg acagtaagaa tttctcaaaa ttagagaatc
     2281 tcaaataccc gtgcggagtt atggtaacgg ttgaaatgac ttcgacaggt aaaggcatgg
     2341 ttccttcatt aattgatttt caggtggcgg aaaaaccgaa aggttgattt atgaaatttg
     2401 aagaacgttt catagttcaa gatttagaaa cgcatgactt tatttatccc gatccgttcg
     2461 gtgacgtggg gtttactcaa aatattaaat cagcaggtca atttgaaagt tacgaagatg
     2521 cattgaactc aggcataaat gaaataggcg gaggattcca aatatttcag ttcttcgtga
     2581 aatcggaata aaagaaaaac aggctcggcg ggcggtctgt caacctttca caaagcccgc
     2641 aacaaaggaa aaatatcatg aaaatgaacc ttgcaacact aattatcggc tgggtggtct
     2701 gtatgtttct ttttcttttc gccatcctct attttatcgg ctaaaaacag gattcgggaa
     2761 agacttcgtc cggatgaagc aagtcaagaa gtcgttttac tttaaacatc aaaaaaagga
     2821 aaaaaacgat gaacatcgtt aaaaaatatg ctgtaaaagc attgttggca gccggtatct
     2881 tcacaccggc tattgttatg gcagatggct ttgatgcatc cgcgattggt acgcaagtgg
     2941 cgaatgtaat catgggtttt gtggcaatgg tttccgccgt gggtatggcg gcaatcaccg
     3001 taattcttgc aatccaaggc ttcaaaatgg cttggagcat gatcaaatct gtcaaataac
     3061 ggcagtgaag aaagaggggg cgcgtaaatg ggctttcgtg tcggtatgaa ttgttttgat
     3121 acaagattgc aggcagacga ctatttattg tcgtcccttc cccctactgt tacccaggac
     3181 ggaaaaatca tcaggccgga aagggtgggc gataaatgga ttttgaacgg aaagccggtc
     3241 acgctgtctt atccgaaatg ttcgaattac gaacaagtta aatccggagc ttatctcggg
     3301 tctatggttt taattctgtt tgtcgttatt tacggcttca ggctgctgat taatttctta
     3361 aaagacatag gcaaagtagg ggcgtgatga tgtttgtcga tttttggttt ttgctcggat
     3421 ttttcctggc tttgtctgtc gctttgatat ttatatgacg tgttttaaaa tcaggctttc
     3481 aaaacaacct ttgaaagaca gaaacatgaa caagccgttt atcacgcagg cgcagttggc
     3541 actttataaa tatcagccgt ccagcaagta ttttgggcag tcgatggcat taattgcgtc
     3601 taaggaattt gaagagtttg taagaaatgt aaaagaatac gacgtaatag aatgtttctc
     3661 ttatttttta aataagaggg taacgcataa tatttggaaa atttattttt ctgatgagtc
     3721 taatattttt attaggaagt cagaagaaaa tggaaaaatt tcgcatgaat ttatttactc
     3781 ggaattttct gatagcaaca ccgattttaa tgtgttgttc tcttagtttt gcagaaccag
     3841 caagaataga tgatcgaata ataaaattta ggccatctaa atcaaagttt tttgaatcta
     3901 caggatatag aaaaatcaat aatgaatttt ctaaattcac agaagcggca aatgtcgaac
     3961 atatccccac gggcgcaaaa gcccgaatca acgcaaagat aaccgccagc gtatcccgcg
     4021 ccgccgtctt gtcaggagtc ggcaaacttg tccgccaagg cgcgaaattc agcacaaggg
     4081 cagtccctta tgtagggaca gcccttttag cccacgacgt atacgaaact ttcaaagaag
     4141 acatacaggc acgaggctac caatacgaca ccgaaaccga caaatttgta aaaggctacg
     4201 aatatagtaa ttgcctttgg tacgaagacg aaagacgtat taatagaacc tatggctgct
     4261 acggcgttga cagttcgatt atgcgcctta tgtccgatga cagcagattc cccgaagtca
     4321 aagaattgat ggaaagccaa atgtataggc tggcacgtcc gttttggaat tggcataaag
     4381 aagaactgaa taaattaagt tctttggatt ggaataattt tgttttaaat cgttgcacat
     4441 ttgattggaa cggcggagat tgtgtggtca ataaaggtga tgattacaga aatggggcta
     4501 atttttccct tagccgcaat ccgaaataca aagaagaaat ggatgccaaa aagctggaag
     4561 agattttatc gttgaaagtc gatgccaatc ccgacaaata cataaaggca accggatatc
     4621 ccggttattc cgaaaaagta gaagtagcac ccggaacaaa agtgaatatg gggcctgtta
     4681 cggacaggaa cgggaatccc gttcaggttg tcgcaacatt cggcagggat tcgcaaggca
     4741 acaccacagt ggatgttcaa gtaatcccgc gttccgacct cacacccgga agcgcggaag
     4801 caccgaacgc acagccgctg cccgaagtat cgcccgccga aaaccccgca aacaacccga
     4861 accccaatga gaaccccggc acgagcccca atcccgaacc cgaccccgat ttgaatcccg
     4921 atgcaaatcc cgatacggac ggacagcccg gcacaagacc cgattccccc gcagttccgg
     4981 gccgcacaaa cggcagggac ggcgaagacg gcggcctttt gtgcaaattc ttccccgaca
     5041 ttctcgcttg cgacaggctg cccgagccca atccggcaga agatttaaat ctgccgtctg
     5101 aaaccgtcaa tgtagagttt cagaaatcag gaatctttca agattccgca cagtgtcccg
     5161 cacctgtcac tttcacagtg actgtgcttg attcaagcag gcagttcgcg ttcagctttg
     5221 agaacgcatg taccatagcc gaacggctaa ggtacatgct tctcgccctt gcttgggcgg
     5281 ttgccgcctt tttttgtatc cgcacagtat ctcgtgaagt ctagcaggcg cagcaccgcc
     5341 gggcttcagt aacttgtacc aaggcagggg gaggacgtcc agaaagattt gtaaagacgg
     5401 ctttatcgtc tttataaatc tttttggata ccccttgccg ccccgccaaa agaacacact
     5461 ctgccgcaag ggcaggtggt aaggcgcgcg ccttttgcgc cgttccccct gcccccgcgg
     5521 cgtcgcaagt gagactgggg gtgtgggggc tagtccccgc aaaatctttc agattaagaa
     5581 acattttttt aatgaggcaa ccgtgccttt taagaaaggg atagcaaatg aaattgttgg
     5641 ccgcattgat tccgctgttg atgagcgtcg caggccgtat attgactgca ttaggcttga
     5701 tggctgttac ttattcgggt gtagatagat tggtagccca ttttcagcag gcgataacca
     5761 gtagcataac gggcgcgcct caagcgatgt tgcagctctt ttatataagc ggtggcggca
     5821 ccgttcttaa tatcctgttt ggcgcgatcg cctttattct gtcattcaaa caaatgacaa
     5881 aactagcaac ctcaatcggg aagaaaaaat aaatggcaga gatctgtttg ataaccggca
     5941 cgcccggttc agggaaaaca ttaaaaatgg tttccatgat ggcgaatgat gaaatgttta
     6001 agcctgatga aaacggcata cgccgtaaag tatttacgaa cataaaaggc ttgaaaatac
     6061 cgcacaccta catagaaacg gacgcaaaaa agctgccgaa atcgacagat gagcagctat
     6121 cggcgcatga tatgtacgaa tggataaaga agcccgaaaa tatcgggtct attgtcattg
     6181 tagatgaagc tcaagacgta tggccggcac gctcggcagg ttcaaaaatc cctgaaaatg
     6241 tccaatggct gaatacgcac agacatcagg gcattgatat atttgttttg actcaaggtc
     6301 ctaagcttct agatcaaaat cttagaacgc ttgtacggaa acattaccac atcgcttcaa
     6361 acaagatggg tatgcgtacg cttttagaat ggaaaatatg cgcggacgat cccgtaaaaa
     6421 tggcatcaag cgcattctcc agtatctata cactggataa aaaagtttat gacttgtacg
     6481 aatcagcgga agttcatacc gtaaataagg tcaagcggtc aaaatggttt tatactctgc
     6541 cagtaataat attgctgatt cccgtttttg tcggcctgtc ctataaaatg ttgagcagtt
     6601 atggaaaaaa acaggaagaa cccgcagcac aagaatcggc ggcaacagaa catcaggcag
     6661 tatttcagga taaaacagaa ggcgagccgg taaacaacgg taaccttacc gcagatatgt
     6721 ttgttccgac attgtccgaa aaacccgaaa gcaagccgat ttataacggt gtaaggcagg
     6781 taagaacctt tgaatatata gcaggctgtg tagaaggcgg aagaaccgga tgcacatgct
     6841 attcgcatca agggacggca ttgaaagaaa ttacaaagga aatgtgcaag gattacgcaa
     6901 gaaacggatt gccgtttaac ccatataaag aagaaagcca agggcgggat gtccagcaaa
     6961 gtgagcagca ccattcggac agaccgcaag ttgccacgtt gggcggaaag ccgtggcaaa
     7021 atcttatgta tgataattgg caggagcgcg gaaaaccgtt tgaaggaatc ggcgggggcg
     7081 tggtcggatc ggcaaactga agaaaacggc aagagagaaa aaagacccgt aaaccgtttg
     7141 aatatagacg gcttacgggt ctttgtttcg cgcaaagcaa gggctaaggc agtcaggcag
     7201 caaatcccgc aatgtattaa aacagacgcg tagaaatgcc ggctgccttt atccatcctc
     7261 aaaattgaat atcatcctag ccgtatcaag gctgtataaa taaggaaaat accaatgaat
     7321 ataatcgggc tggacatctc aaaggacacc atagacgcaa cattgcataa aacaaacgga
     7381 agtatccatt acattaaatt taagaataat gatgatggat taaaacagtt tagattgtgg
     7441 ataaagggaa acagaatcag aaaagtctat atcggcatgg aggcaacagg catctattac
     7501 gaaaaggcag cagatatgct ttcttcctac tatactgttt acgttattaa tcccttaaaa
     7561 atcaaggact acggaaaaag caggtttaac cgtaccaaaa ccgacaaagc agattcaaac
     7621 ctgatagcag actacataaa aaggcatcaa gatacattga taccgtatca gatacccaaa
     7681 aacaaagcac tgcaaaaact gattaatctt aaaaaccaat tacagcaaca gcagaagcaa
     7741 attaaaaacc gtcttcatag cactgaagaa gacttcataa ggaacataca tcaagacttg
     7801 atagatacca tacaggacaa gatggaacag gtaaaaatag ccatatccga acaaatcaaa
     7861 aaacaaacgg actataacca ttaccgcaat cttcaaacca tcccgagcat aggcaaagac
     7921 accgcatcag ttctttatgc gcaactgaca gaaaaacatt ttaaaaccgc aaaccagttt
     7981 gtatcctatg ccggattaag tcccgccatc atacaatcag ggacaagcgt aagaggtcgg
     8041 ggcagattga gccgatacgg aaacagacga ttaaaaagta cgctgtatat gcccgccctt
     8101 tgtgcttacc gttttaacgc atttccgaaa ttaataaata atctgaaaaa agcgggtaag
     8161 ccaaagatgg taattatcgt tgccatcatg cgcaaactgg cgaagctcgc ctattatatt
     8221 gttaaaaccg gccagcctta cgatgcggaa agacaccgat tgaatcaata aaattcaaca
     8281 aaattaaacg gttacgcgaa tatatttgtg taaccgtgca tttgcatatc gtaaataaac
     8341 gtaaataaaa ataacaatat aaatcagtat attgcaactt tgttttttat tttgtgttga
     8401 cgggcaacat atcatctgcg cgggaatgac gggtttcgag attgcggtgt tgtcggaacg
     8461 caactgaatc gtcattccca cggaagtggg aatctagaat ctcgggggtt cagtcatttc
     8521 cgatagattc ccgccgcgtc a
//
LOCUS       DAXHSF010000082         8322 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.082, whole genome shotgun sequence.
ACCESSION   DAXHSF010000082 DAXHSF010000000
VERSION     DAXHSF010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8322
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.082"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..192
                     /locus_tag="ACFM8S_001300"
     CDS             <1..192
                     /locus_tag="ACFM8S_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HFC6314367.1"
                     /translation="HGFYVLSDTTELTYKCTDYYKPEAEQVLIWNDPAIGIDWPLQGE
                     PNLSPKDLAGKVLAEAVTF"
     gene            216..2330
                     /locus_tag="ACFM8S_001301"
     CDS             216..2330
                     /locus_tag="ACFM8S_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HFC6314368.1"
                     /translation="MFLFSDGLQSINNNNRRKRIVKNAYIPSRGIRKIPHLSTLLPEF
                     HIYKDGKGAEAVVGWGLRPTTHKARAFATEHQLPFIALEDGFLRSLGLGVSGYPPYSI
                     VYDDIGIYYDTTRPSRLEQLILAADTMPSETLAQAQQTMDFILQHHLSKYNHAPELSD
                     DHPLRSPSKPETVLIIDQTFGDMAIQYGGADASTFELMFQTALNENPQADIWVKTHPD
                     VLCGKKQGYLTQLAQQHRVHLLAEDINPISLLQNVDKVYCVTSQMGFEALLCGKPLTT
                     FGLPWYAGWGVSDDRHPEINRLVQTQRRATRNLLQLFAAAYLQYSRYLNPNTGEAGSL
                     FDVIDYLATVKRKNDKLRGELYCVGMSLWKRAVAKPFFNVPSCRLKFISSTQKLAGVK
                     LSDDARILAWGNGKEAIVRFAEQHHIPLLRMEDGFIRSVGLGSNLVPPLSLVTDDMGI
                     YFNAEAPSRLEHILQNQNFDDQDFQTALKLQKMLTENHISKYNVGNSDFTAPSTDKTV
                     ILVPGQVEDDASIRYGSPQIYHNLDLLRTVRERHPDAYIIYKPHPDVVSGNRIGHISP
                     EDAARYADQTAEQADILTCLQYADEIHTMTSLTGFEALLRGKKVSCYGLPFYAGWGLT
                     QDLLPIPRRSRRLELWQLIAGTLIHYPDYIHPETHQAINAETAAQILIRQKNMQKNNN
                     GLHRGCFAKKLGKIKQLYRSFK"
     gene            2467..3726
                     /locus_tag="ACFM8S_001302"
     CDS             2467..3726
                     /locus_tag="ACFM8S_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HFC6314369.1"
                     /translation="MKQTVLKNNLQNLLESAENILLLQGPVGDFFLRLADWLTANGKT
                     VHKFNFNAGDDYFYPPTQAHTVVFNDNYDAFPEFLQEYITQHHIQAVVCFGDTRPYHV
                     IAKRIANENQASFWAFEEGYFRPYYITLEKDGVNAFSPLPRRADFFLEQFPKLAQQEY
                     KAPTPVHGGFTPMAKNAIRYYIELFRNPRKYPDYIHHRAPNAGHYLKPWSLSILKRLN
                     YYIEDIQIAKRVEAGKYGKFFIVPLQVFNDSQVRIHCDFPSVRSFLLHVLASFAAHAP
                     ADTNLIIKHHPMDRGFIDYSRDIKRFIKKHPKLKGRITYVHDVPLPVFLRHGLGMVTI
                     NSTSGLSGLIHNMPVKVLGRAHYDIPGITDQNTLAEFWNHPTPPDKELFHAYRMYHLN
                     VTQINGNFYSQVFFPNKKTSDSSTPAT"
     gene            3902..5308
                     /locus_tag="ACFM8S_001303"
     CDS             3902..5308
                     /locus_tag="ACFM8S_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC6314370.1"
                     /translation="MTAAPDILYRQAAALLEQSNTAQALPLLQQAAEQGYAEAAFVLG
                     NHLLQNGQPEQALSWLEAAAAQRHPKALFSLLQQREHNGTPTGQLLNDYAWLGEQGHS
                     EAQLILMRYHAQRNDPQSLYWAELAAARYAAPAYYHLARHHQRQGDVETAIEQYEKAA
                     ALGVTAACWQLGQIYFYGTGVSPNHAQAEHYLEPAAQAGHIAAQTLLADLLAAQRKPE
                     ALEWYRRAADKEQAEAQSKLAQYALTGELSERDPFQAARYAKAAAEKNHPEALKIMGD
                     LYRYGLGIKADNHIAQDYYHRAAALGSAAAAQKLISDAALYHPQQYEQIKTAALQQQQ
                     TETIYRLAEAQACAIGRPADYNAARKNYMEAAGFHHKNAAAALGRIYHYGLGTAQDPR
                     AAAHWYAIAAEQNHPSAQYHLACFYYHGQGVGCHVPTACYWLQAAIGNGHTSAESLIS
                     LLEQWRREAHHAIGQKAV"
     gene            5526..6740
                     /gene="gltS"
                     /locus_tag="ACFM8S_001304"
     CDS             5526..6740
                     /gene="gltS"
                     /locus_tag="ACFM8S_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFC6314371.1"
                     /translation="MEWEFNSYYTLIAATLVLLVGKFLVQKIKFLRDFNIPEPVAGGL
                     VAAIILFALHEAYGVSFKFEKPLQNAFMLIFFTSIGLSADFSRLKAGGLPLVVFTAIV
                     GGFILVQNFVGVGLATALGLDPLIGLITGSVSLTGGHGTSGAWGPNFETQYGLVGATG
                     LGIASATFGLVFGGLIGGPVARRLINKMGRKPVENKKQDQDDNADDVFEQAKRTRLIT
                     AESAVETLAMFAACLAFAEIMDGFDKEYLFDLPKFVWCLFGGVVIRNILTAAFKVNMF
                     DRAIDVFGNASLSLFLAMALLNLKLWELTGLAGPVTVILAVQTVVMVLYATFVTYVFM
                     GRDYDAAVLAAGHCGFGLGATPTAVANMQSVTHTFGASHKAFLIVPMVGAFFVDLINA
                     AILTGFVNFFKG"
     gene            6839..7855
                     /locus_tag="ACFM8S_001305"
     CDS             6839..7855
                     /locus_tag="ACFM8S_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFC6314372.1"
                     /translation="MYRKLIALPFALLLAACGREEPPKALECANPAVLQGIRGNIQET
                     LTQEARSFAREDGRQFVDADKIIAAAYGLAFSLEHASETQEGGRTFCIADLNITVPSE
                     TLADAKANSPLLYGETALSDIVRQKTGGNVEFKDGVLTAAVRFLPVKDGQTAFVDNTV
                     GMAAQTLSAALLPYGVKSIVMIDGKAVKKEDAVRILSGKAREEEPSKPTPEDILEHNA
                     AGGDAGVPQAAEGAPEPEILHPDDGERADTVTVSRGEVEEARVQNQRAESEITKLWGG
                     LDTDVQKELVGEQRKWAQEKISNCRQAAAQADRQEYAEYLKLQCDTRMTRERIQYLRG
                     YSID"
     gene            7866..8063
                     /locus_tag="ACFM8S_001306"
     CDS             7866..8063
                     /locus_tag="ACFM8S_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314373.1"
                     /translation="MNTVPKSRIPVKPLPEKTTDEAKVEKWRQLGAEHGLSGEWAVAA
                     RLGENGFTEEQMENIANLFGR"
     gene            8081..8230
                     /locus_tag="ACFM8S_001307"
     CDS             8081..8230
                     /locus_tag="ACFM8S_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314374.1"
                     /translation="MPSETLLSVSDGILTNTVRRRKTAVFSCVSYADVSTHKTAHKAL
                     PYVPS"
BASE COUNT         2100 a         2459 c         1933 g         1830 t
ORIGIN      
        1 cacgggtttt acgttttgag cgacacgacc gaacttacct acaaatgcac ggactattac
       61 aagcctgaag ccgaacaggt tttaatatgg aacgacccgg caatcggcat cgactggccg
      121 ctacaaggcg agccgaacct gtcgcctaaa gacttggcag gcaaagtatt ggccgaagca
      181 gtcacgtttt aagactattc aggccgtctg aaagaatgtt cctcttttca gacggcctac
      241 aatccattaa taacaataat cgacgaaaac gcattgtgaa aaacgcctac atcccctctc
      301 gtggcatccg caaaatcccc catctctcca ccctattgcc cgagtttcat atctacaaag
      361 acggaaaagg agcagaggct gttgtcggct ggggtttgcg cccgacgaca cacaaagcgc
      421 gtgcttttgc caccgaacac cagcttccct ttattgcttt ggaagacggc tttttacgct
      481 cgctcggact gggcgtctcc ggttatcccc cctactccat cgtctatgac gacatcggca
      541 tctactacga cactacccgc ccttctcgtt tggaacaact gattcttgcc gccgatacca
      601 tgccgtctga aaccttggct caggcgcaac agacgatgga tttcatcctg caacaccacc
      661 tgtccaaata caaccacgcg cccgaacttt cagacgatca tcctttacgc tccccatcca
      721 agcctgaaac ggtcctcatt atcgaccaaa ccttcggcga tatggccatc caatatggcg
      781 gcgcagacgc ctctacgttt gaactgatgt ttcagacggc cttgaatgaa aacccgcaag
      841 ccgatatctg ggtaaaaacc catcccgatg ttttgtgcgg caaaaaacaa ggctatctga
      901 cccaactggc gcagcaacac cgcgtccatc ttctggcaga agacatcaac ccgatttctt
      961 tgttgcaaaa cgtcgataaa gtttattgcg ttacctcgca aatgggtttt gaggcacttt
     1021 tgtgcggcaa gccgctgacc accttcggcc tgccgtggta tgccggatgg ggtgtaagcg
     1081 acgaccgcca tcctgaaatc aaccgccttg ttcaaaccca acgccgcgcc acccgcaact
     1141 tgctgcagct cttcgccgca gcctatctgc aatacagccg ctacctcaat cccaataccg
     1201 gcgaagcagg cagcctcttt gatgtcatcg actatctggc gacggtcaaa cgtaaaaacg
     1261 acaaattgcg tggcgagtta tattgcgtcg gtatgtcttt gtggaaacgc gcggttgcca
     1321 aaccgttctt taacgtaccc tcttgccgtc tgaaatttat ctcttccacc caaaaactgg
     1381 ccggggtcaa actgtccgac gatgcacgca tcctggcttg gggcaacggc aaagaggcca
     1441 tcgtccgctt tgccgaacaa caccacatcc ccctgctgcg catggaagac ggctttatcc
     1501 gctcggtcgg actcggctcc aacttagtgc cgccgctgtc gctcgttacc gacgatatgg
     1561 gtatttactt caatgccgaa gccccatccc gccttgaaca catcctgcaa aaccaaaact
     1621 tcgacgatca agactttcag acggccttga agttgcaaaa aatgctgacc gaaaaccata
     1681 tcagcaaata caacgtcggc aactcagact ttaccgcccc atcaaccgac aaaaccgtga
     1741 tcctcgttcc cggtcaggtt gaggatgatg cgtctatccg ctatggttcg ccccaaatct
     1801 atcacaatct ggatttgctc cgtaccgtac gcgaacgcca tcccgatgcc tatatcatct
     1861 acaaaccgca tcccgatgta gtcagcggta accgcatcgg ccatatttcc cctgaagatg
     1921 ctgcacgata tgccgaccaa accgccgaac aagccgacat cctgacctgt ctccaatacg
     1981 cagacgaaat acataccatg acttcgctga ccggttttga agccttgttg cgcggcaaaa
     2041 aagtcagctg ctacggcctg cctttttacg caggctgggg gcttacccaa gatctgctcc
     2101 ccatcccgcg ccgtagccgc agacttgagc tttggcagct gattgccggc acgctcatcc
     2161 actatcccga ctacatccac cccgaaaccc atcaggccat caatgcggaa accgctgccc
     2221 aaatcctgat acgacaaaaa aatatgcaaa aaaacaacaa cggattacat cgcgggtgct
     2281 ttgccaaaaa attaggtaaa atcaaacaac tatatcgatc tttcaaataa ataccatcaa
     2341 agttaacgat gcgtcataaa cttgcctcta ttgcggcatc attgcctttg catcgttaat
     2401 tctcttggcg tatgcttgaa agttcaacct aaaactatta cataaaaaac aaaaccacat
     2461 tgcaacatga aacagaccgt cctcaaaaat aacctgcaaa acctgcttga aagcgcagaa
     2521 aatatcctgc tgcttcaagg ccctgtcggc gatttttttc tgcgccttgc cgactggctg
     2581 actgcaaacg gcaaaaccgt acataaattc aactttaatg caggcgacga ctatttttat
     2641 ccgcccactc aagcgcatac cgttgttttt aacgacaact acgatgcctt tcctgagttt
     2701 ttgcaagaat acatcactca acatcacatc caggccgttg tctgctttgg cgacacacgc
     2761 ccttatcacg tcattgcaaa acgcattgca aacgaaaacc aagccagttt ctgggcgttt
     2821 gaagaaggct atttccgccc ctactacatc accttagaaa aagacggcgt caacgcattt
     2881 tccccgttgc cgcgccgtgc cgactttttt cttgaacaat tccctaagct tgcccagcaa
     2941 gaatataaag cgccaacgcc ggtacacggc ggttttacgc ccatggcaaa aaacgctatc
     3001 cgttactata tcgagttgtt ccgcaatcca cgcaaatacc ccgactacat ccaccaccgc
     3061 gcacccaatg ccggccatta cctcaaacca tggtcgctct ccatcctcaa gcgtttgaac
     3121 tactatattg aagacatcca aatcgccaaa cgtgtggaag caggcaaata cggcaagttt
     3181 tttattgttc ccttacaggt attcaacgac agccaagtcc gtatccattg cgactttccc
     3241 agcgtccgca gcttcctgct ccatgttttg gcctcctttg ccgcgcacgc gcctgccgat
     3301 accaacctca tcatcaagca tcatccgatg gaccgcggct ttatcgacta ctcgcgcgac
     3361 atcaaacgct ttatcaaaaa acaccccaaa ctcaaaggcc gtatcactta cgtccatgat
     3421 gtccccctgc ccgttttcct gcgccacggc ctcggcatgg ttaccatcaa cagcaccagc
     3481 ggcctgtccg gcttgatcca caacatgcca gtcaaagtcc tcggccgcgc ccattacgac
     3541 attcccggca taaccgacca aaataccttg gcagaatttt ggaatcatcc gacaccgcct
     3601 gacaaagagc tgttccatgc ctaccgaatg taccacctca acgtgaccca aattaacggc
     3661 aacttctaca gtcaggtctt ttttcctaac aaaaaaacct ccgactcttc cactccagca
     3721 acctgactta gcaaaaataa ttcaggccgt ctgaaaacat ttcagacggc ctgaaacaat
     3781 caatacctta gctactgcca tgtaaataaa acacaaaaac ctatttttac aaagccctca
     3841 accggtatcg ccgcccatat gccgcagcat ccgtcttcca ctttatatcc gcccgcaaac
     3901 catgaccgcc gctcctgata tcctctaccg gcaagccgcc gcccttttgg aacaatccaa
     3961 taccgcccaa gccctgcccc tgttgcaaca ggcggcagag caaggttatg cggaagctgc
     4021 tttcgtattg ggcaaccatc tgctgcaaaa cggccaaccg gagcaggcac tttcatggtt
     4081 ggaagccgcc gcggcccaac gccatcccaa agcactcttc tccctgctgc aacaacgcga
     4141 acacaacggc accccgaccg gacagcttct caacgactat gcctggctgg gtgagcaggg
     4201 gcactcagaa gcccaattaa tcctcatgcg ttaccacgcg caacgcaacg atccacaatc
     4261 gctctactgg gcggaacttg ctgccgcccg atatgccgca cctgcgtatt accatctggc
     4321 acgccatcat caacgccaag gcgacgttga aacagccatc gaacaatacg aaaaagcggc
     4381 agcactcggc gtaactgccg cctgctggca acttggtcaa atctacttct acggtacagg
     4441 tgtcagcccc aaccacgcac aagccgaaca ctatctcgaa ccagccgcac aagccggcca
     4501 catcgccgca caaacgctgc tggctgacct tcttgccgcc caacgcaaac ctgaagcctt
     4561 ggaatggtat cgtcgtgccg ccgataagga acaagcggaa gcacagtcta agctggccca
     4621 atacgccctg accggcgaac tttccgaacg cgatccgttc caagcggcac gatatgccaa
     4681 agccgctgcc gagaaaaacc atcctgaagc cctgaaaatc atgggcgacc tctaccgcta
     4741 cggtctcggt atcaaagccg acaaccatat cgcgcaagat tactaccacc gtgccgccgc
     4801 gctgggttct gccgccgcag cacaaaaact catcagcgac gccgcgctgt accatccgca
     4861 acaatacgaa caaatcaaaa ctgccgccct gcaacaacaa caaaccgaaa ccatctaccg
     4921 tttggcggaa gcacaagcct gcgccatcgg ccgtcccgcc gactacaatg ccgcgcgaaa
     4981 aaattacatg gaagctgccg ggttccacca taaaaacgca gcggcagcct taggccgcat
     5041 ctaccattac ggcctcggta cggcgcaaga tcctcgggcg gctgcacact ggtacgccat
     5101 tgctgccgaa caaaaccacc cttccgccca ataccacctc gcctgttttt actatcacgg
     5161 gcaaggtgtc ggctgtcatg ttccgaccgc ctgctactgg ctgcaggccg ccatcggcaa
     5221 cggccacact tcggccgaat cattaatatc cctattagaa caatggcgac gcgaagcaca
     5281 ccatgccatc ggacaaaagg ccgtctgaaa agatttacac tcgcattttt tgacaatctt
     5341 taactattcc cctaatattt gccagttatt tttcacggac acgccattgt tttcatttct
     5401 ttctgaaaac accttgtccg cgcatcaata ccatgacact cggcggataa cgccaagcgt
     5461 tgaaacacac tacatccgga acaaaaacgg atgctcggaa aaatatttct aggaggtgaa
     5521 acaacatgga atgggaattc aacagttatt acacactgat tgccgccacg ctcgtgttgc
     5581 tggttggtaa atttctggtt caaaaaatca aattcttacg agacttcaat attcccgagc
     5641 cggtcgctgg cggtttggtt gccgcgatta tcctgtttgc gctgcacgag gcgtacggcg
     5701 tgagcttcaa atttgagaaa ccgctgcaaa atgcgtttat gctgattttc ttcacgtcca
     5761 tcggcttgag cgcggatttt tcccgtttga aggcgggcgg tttgccgcta gtggttttta
     5821 ccgcgattgt gggcggattt atcttggtgc aaaactttgt cggggtcgga ctggctacgg
     5881 ctttgggttt ggacccgctc atcggtctga ttaccggttc ggtgtcgctg acgggcggac
     5941 acggtacgtc aggtgcgtgg ggacctaatt ttgaaacgca atacggcttg gtcggcgcaa
     6001 ccggtttggg tattgcatcg gctactttcg ggctggtgtt cggcggcctg atcggtgggc
     6061 cggttgcgcg ccgcctgatc aacaaaatgg gccgcaaacc ggttgaaaac aaaaaacagg
     6121 atcaggacga caacgcggac gacgtgttcg agcaggcaaa acgcacccgc ctgattacgg
     6181 cggaatctgc cgttgaaacg cttgccatgt ttgccgcgtg tttggcgttt gccgagatta
     6241 tggacggctt cgacaaagaa tatctgttcg acctgcccaa attcgtgtgg tgtctgtttg
     6301 gcggcgtggt catccgcaac atcctcactg ccgcattcaa ggtcaatatg ttcgaccgcg
     6361 ccatcgatgt gttcggcaat gcttcgcttt cgcttttctt ggcaatggcg ttgctgaatt
     6421 tgaaactgtg ggagctgacc ggtttggcgg ggcctgtaac cgtgattctt gccgtacaaa
     6481 ccgtggtgat ggttttgtac gcgacttttg ttacctatgt ctttatgggg cgcgactatg
     6541 atgcggcagt attggctgcc ggccattgcg gtttcggctt gggtgcaacg ccgacggcgg
     6601 tggcaaatat gcagtccgtc acgcatactt tcggcgcgtc gcataaggcg tttttgattg
     6661 tgcctatggt cggcgcgttc ttcgtcgatt tgattaatgc cgcgattctc accggttttg
     6721 tgaatttctt taaaggctga ttttccgcct ttccgacaaa gcacctgcaa ggtttaccgc
     6781 ctgcaggtgc ttttgctatg atagccgcta tcggtctgca ccgtttggaa ggaacatcat
     6841 gtatcggaaa ctcattgcgc tgccgtttgc cctgctgctt gccgcttgcg gcagggaaga
     6901 accgcccaag gcattggaat gcgccaaccc cgccgtgttg caaggcatac gcggcaatat
     6961 tcaggaaacg ctcacgcagg aagcgcgttc tttcgcgcgc gaagacggca ggcagtttgt
     7021 cgatgccgac aaaattatcg ccgccgccta cggtttggcg ttttctttgg aacacgcttc
     7081 ggaaacgcag gaaggcgggc gcacgttctg tatcgccgat ttgaacatta ccgtgccgtc
     7141 tgaaacgctt gccgatgcca aggcaaacag ccccctgctg tacggggaaa ccgctttgtc
     7201 ggatattgtg cggcagaaga cgggcggcaa tgtcgagttt aaagacggcg tattgacggc
     7261 agccgtccgc ttcctacccg tcaaagacgg tcagacggca tttgtcgaca acacggtcgg
     7321 tatggcggcg caaacgctgt ctgccgcgtt gctgccttac ggcgtgaaga gcatcgtgat
     7381 gatagacggc aaggcggtga aaaaagaaga cgcggtcagg attttgagcg gaaaagcccg
     7441 tgaagaagaa ccgtccaaac ccacgcccga agacattttg gagcacaatg ccgccggcgg
     7501 cgatgcgggc gtaccccaag ccgcagaagg cgcgcccgaa ccggaaatcc tgcatcctga
     7561 cgacggcgag cgtgccgata ccgttaccgt atcacgggga gaagtggaag aggcgcgcgt
     7621 acaaaaccag cgtgcggaat ccgaaattac caaactttgg ggaggactcg ataccgacgt
     7681 gcaaaaagag ttggtcggcg aacaacgcaa gtgggcgcag gaaaaaatca gcaactgccg
     7741 acaagccgcc gcgcaggcag accggcagga atacgccgaa tacctcaagc tgcaatgcga
     7801 cacgcggatg acgcgcgaac ggatacagta tcttcgcggc tattccatcg attaggggca
     7861 aaccgatgaa taccgtccca aaaagcagga ttcccgtcaa accgctgccc gaaaaaacca
     7921 cagacgaagc caaagtcgaa aaatggcggc agctcggtgc ggaacacggt ttgtcgggcg
     7981 aatgggcagt tgccgccaga ttgggcgaaa acggttttac cgaagaacag atggaaaata
     8041 tcgccaacct gttcggcaga taaagagaaa attgacggaa atgccgtctg aaaccctgtt
     8101 gtcggtttca gacggcattt tgaccaatac ggtacgcagg cgcaaaacag ccgtcttttc
     8161 ctgcgtttcc tatgctgatg tttcaacaca caagacggca cataaagcgt tgccctatgt
     8221 gccgtcctga ttcggaaggg gttacgcccc tcccaaatat agtggattaa caaaaaccag
     8281 tacggcgttg cctcgcctta gctcaaagag aacgattctt ta
//
LOCUS       DAXHSF010000083         8270 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.083, whole genome shotgun sequence.
ACCESSION   DAXHSF010000083 DAXHSF010000000
VERSION     DAXHSF010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8270
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.083"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(<1..216)
                     /locus_tag="ACFM8S_001308"
     CDS             complement(<1..216)
                     /locus_tag="ACFM8S_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC6314375.1"
                     /translation="MKITHCKLKKKVQKELLHFFVLEVTARSAADILGIHPNSAALFY
                     RKIRTVINYHLALAADEVFEGSVELDES"
     gene            625..723
                     /locus_tag="ACFM8S_001309"
     CDS             625..723
                     /locus_tag="ACFM8S_001309"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="eamA-like transporter family protein"
                     /protein_id="HFC6314376.1"
                     /translation="MAVVFLDERPNTQEWIGLGLVTAGVLTLALKR"
     gene            complement(794..2179)
                     /locus_tag="ACFM8S_001310"
     CDS             complement(794..2179)
                     /locus_tag="ACFM8S_001310"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFC6314377.1"
                     /translation="MVSIFDIFKIGIGPSSSHTVGPMKAAAAFATGLDAQAVRIVIDI
                     YGSLALTGYGHGTFDALMLGLEGSLPHDIPLAGIPERLERIRTQHILRLNGQEIRFIP
                     DRDLNILGNQVLPKHPNGLRFTAYASDGTVLNEQVYYSVGGGFVVTEEDFDRQAETEK
                     AVPYPYTSCAELLARCRLNRLDISEVVLANEAALAGCGEAEIRRRAAAVAEVMEGCIK
                     RGLGADGELPGGLNVRRRAPQLAAKLKVLRETEIVNTQLWPMVYAMAVNEENAAGGRV
                     VTAPTNGAAGIIPAVLHYFRKFNPHATQERVENFLLTAGAIGILYKTNASISGADVGC
                     QGEVGVACSMAAGAYAEVIGGTPKQVENAAEMAMEHHLGLTCDPVGGLVQIPCIERNG
                     IAAEKALKLGTLALLEDGTDKKVSLDEVIRTMLQTGRDMKATYKETSLAGLAATLRKK
                     AVPVSVRVVEC"
     gene            complement(2278..4668)
                     /gene="gyrB"
                     /locus_tag="ACFM8S_001311"
     CDS             complement(2278..4668)
                     /gene="gyrB"
                     /locus_tag="ACFM8S_001311"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFC6314378.1"
                     /translation="MTEQKHEEYGADSIQVLEGLEAVRKRPGMYIGDTQDGSGLHHMV
                     FEVLDNAIDEALAGHCDKITVTIHADHSVSVADNGRGMPTGIHPKEGRSAAEVIMTVL
                     HAGGKFDNNSYKISGGLHGVGVSVVNALSDWVTLTIYRDGKEHFVRFVRGETEEPLKV
                     VGDSDKKGTTVRFLASTETFGNVEYSFDILAKRIRELSFLNNGVDIELTDERDGKHES
                     FALSGGVAGFVQYMNRKKTPLHEKIFYAFGEKDGMSVECAMQWNDSYQESVQCFTNNI
                     PQRDGGTHLTALRQVMTRTINSYIEANEVAKKAKVETAGDDMREGLTCVLSVKLPDPK
                     FSSQTKDKLVSSEIGPVVNEVINQALTDFLEENPNEAKIITGKIVDAARAREAARKAR
                     EITRRKGVMDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAMQGRDRKFQAILPLK
                     GKILNVEKARFEKMLASQEVAALITALGAGIGKEEFNAEKLRYHRIIIMTDADVDGAH
                     IRTLLLTFFYRQMPELVERGYIYIAQPPLYKAKYGKQERYLKDELEKDQWLLGLALEK
                     AKIISDGRTIEGAELADTAKQFLLAKTVIEQESRFVDELVLRAMLHASPIDLTSSENA
                     DKAVAELSGLLDEKEVALERIEGHEGHRFIKITRKLHGNVMVSYIEPKFLNSKAYQTL
                     TQTAAALKGMVGEGAKLYKGENGYDADSFETALDILMSVAQKGMSIQRYKGLGEMNPE
                     QLWETTMDPAVRRLLKVRIEDAIAADEVFVTLMGDEVEPRRAFIENNALIAQNIDA"
     gene            4938..5810
                     /locus_tag="ACFM8S_001312"
     CDS             4938..5810
                     /locus_tag="ACFM8S_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HFC6314379.1"
                     /translation="MEIWNMLDTWLGAVPIRAEAVESVAVVAALLLARALLLNIHFKR
                     HPDFGIESKRRFLVASRNITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVVVATKELI
                     MCLSGSILRSATQQYSVGDYIEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVS
                     FPNSLLLSHPVRRDNILGDYVIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQ
                     RHLENVQAEKLFITPAARPRVTRVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRV
                     QYRLLNYPAGSETL"
     gene            5898..7934
                     /locus_tag="ACFM8S_001313"
     CDS             5898..7934
                     /locus_tag="ACFM8S_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HFC6314380.1"
                     /translation="MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQ
                     THTGWANTVEPLTGITERVGRIWGVVSHLNSVADTPELRAAYNELMPEITAFFTEIGQ
                     DIALYNRFKTIKNSPEFATLSTAQKTKLNHDLRDFVLSGAELPPEQQAELAQLQTEGA
                     QLSAKFSQNVLDATDAFGIYFDDAAPLAGIPEDALAMFAAAAQSEGKTGYKIGLQIPH
                     YLAVIQYADNRELREQIYRAYVTRASELANDGKFDNTANIDRTLANALQTAKLLGFKN
                     YAELSLATKMADTPEQVLNFLHDLARRAKPYAEKDLAEVKAFARESLNLADLQPWDLG
                     YASEKLREAKYAFSETEVKKYFPVGKVLNGLFAQIKKLYGIGFTEKTVPVWHKDVRYF
                     ELQQNGETIGGVYMDLYAREGKRGGAWMNDYKGRRRFSDGTLQLPTAYLVCNFAPPVG
                     GREARLSHDEILILFHETGHGLHHLLTQVDELGVSGINGVEWDAVELPSQFMENFVWE
                     YDVLAQMSAHEETGVPLPKELFDKMLAAKNFQRGMFLVRQMEFALFDMMIYSEDDEGR
                     LKNWQQVLDSVRKEVAVVRPPEYNRFANSFGHIFAGGYSAGYYSYAWAEVLSADAYAA
                     FEESGDIAATGKRFWQEILAVGGSRSAAESFKAFRGREPSIDALLSHSGFDNAA"
BASE COUNT         1802 a         2292 c         2262 g         1914 t
ORIGIN      
        1 gctttcgtcc agctcgacag aaccctcaaa aacctcatcg gcagccaagg ccaaatgata
       61 gttgataacc gtgcggattt tacggtagaa cagtgctgcc gaattgggat ggatacccaa
      121 aatatcggcg gcagaacggg cggtaacttc cagtacaaaa aaatggagta gttctttctg
      181 tacttttttc tttaatttgc agtgcgttat cttcatattt cgagggtaac atatctgcta
      241 atctagtaca gccccttgaa aaatgtttgt ttccggattt tgccattccc atccggtttt
      301 gcgctgtacg atgtgtttta gcgcggactt gctcaaaatc ggatgtggtt tcggtatttg
      361 aggctttgat tagggatgcg gactttcaat atattttctc agctacaaca acgaaggctt
      421 gatgtctgtc gggcaggtaa gggagatttt tgagcgtttc ggcaaatatg atttggttca
      481 aacggaatac cggcggttta aggcagataa gacagaaaac cgtaatcata aggcaaattc
      541 gacattcgaa tatctgcata ttttagaaaa gaccttttaa aaattcttgc cggatgctgc
      601 aaacaacgcc ggtttgcatt cctgatggcg gtggttttct tagacgaacg cccgaacacg
      661 caggaatgga taggcttggg gctggttacg gcgggcgtgt tgacgctggc actgaaacgg
      721 taaagccgca agaaataaat gaaatgccgt ctaaaaaact gttttcagac ggcattttcg
      781 tttctgtcca tcctcagcac tcgaccacgc gcacggatac ggggacggct tttttccgga
      841 gcgtggcggc gagtccggca agcgaggttt ctttgtaggt cgccttcata tcccggccgg
      901 tttgcagcat ggttcggatg acttcgtcga gcgagacttt tttgtccgtg ccgtcttcca
      961 agagcgcgag ggtgccgagt ttgagggctt tttcggcggc gatgccgttg cgctcgatgc
     1021 aggggatttg caccagtccg ccgacggggt cgcaagtcag ccccaaatgg tgttccatcg
     1081 ccatttcggc ggcgttttcc acttgtttgg gcgtgccgcc gatgacttcg gcgtatgcgc
     1141 ccgccgccat cgaacacgct acgccgactt cgccctgaca gccgacatcc gcaccggaaa
     1201 tggaggcgtt ggtcttgtag aggatgccga ttgcgcctgc ggtgagcagg aagttttcga
     1261 cgcgttcctg tgtggcgtgc ggattgaact tgcggaaata gtgcaatacg gcgggaatga
     1321 tgcctgccgc gccgttggtc ggggcggtaa cgacgcgtcc gccggcggcg ttttcttcgt
     1381 tgaccgccat ggcgtacacc atcggccaga gctgggtgtt gacgatttcg gtttcgcgca
     1441 ggactttgag cttggcggca agctgcgggg cgcggcggcg gacgttcaat ccgccgggca
     1501 gttcgccgtc cgcacccaag ccgcgtttga tgcagccttc cataacctcg gcaacggcag
     1561 cggcgcggcg gcggatttcg gcttcgccgc atccggcaag cgcggcttcg tttgccaaca
     1621 cgacttcgga gatgtcgagc cggttcagac ggcatcgggc aagcagttcg gcgcaactgg
     1681 tatagggata gggaacggct ttttccgttt ccgcctgccg gtcaaaatct tcttcggtaa
     1741 cgacaaagcc gccgccgacc gaataataaa cctgttcatt caataccgtg ccgtctgaag
     1801 cataggcggt aaaacgcagg ccgttggggt gtttgggcag cacttgattg ccgagtatgt
     1861 tcaggtcgcg gtcggggatg aagcggattt cttgcccgtt gagccggagg atgtgctgcg
     1921 tgcggatgcg ttcgaggcgt tcgggaatgc cggcaagcgg gatgtcgtgc ggcaggctgc
     1981 cttccaaacc gagcatcagc gcgtcaaatg taccgtgtcc gtatccggtc agtgcgagcg
     2041 agccgtaaat gtcgatgacg atgcgaacag cctgtgcatc caaacctgtc gcaaaggcgg
     2101 cggctgcctt catcgggccg accgtatgcg aactggaagg cccgataccg attttgaaaa
     2161 tatcaaaaat gctgaccatg gttttgctcc gaaaggcggt ttcagacggt atggcttccg
     2221 tttgacaaac caaaaggaga cgcggcacga tgcccgtctc cttttttaaa acggcactta
     2281 tgcgtcgata ttttgggcaa tcagcgcgtt gttttcgata aaggcgcggc gcggttcgac
     2341 ctcgtcgccc atcagggtaa cgaatacttc gtcggcggca atggcgtctt cgatgcgcac
     2401 tttcaacaga cggcgcacgg cgggatccat cgtggtttcc cacagctgct cggggttcat
     2461 ctcgcccaag cctttgtatc gttggatgga catacctttt tgggcaacgc tcatcaagat
     2521 gtccaaagcg gtttcaaagc tgtccgcgtc gtacccgttt tcgcctttgt aaagcttggc
     2581 accctcgccg accatgcctt tgagcgcggc ggcggtttgg gtgagggttt ggtaggcttt
     2641 gctgttgagg aacttgggtt cgatgtagct gaccatgacg ttgccgtgca gcttgcgcgt
     2701 gattttgatg aaccggtgtc cttcatgacc ttcgatgcgt tcgagggcga cttctttttc
     2761 gtcaagcaga ccggaaagtt cggcaacggc tttatcggcg ttttcagacg acgtcaaatc
     2821 aatgggcgac gcgtgtagca tggcgcgcag gacgagttcg tctacgaagc ggctttcctg
     2881 ttcgatgacg gtttttgcca acaggaattg tttggcggtg tcggcaagtt ctgcgccttc
     2941 gatggtgcgg ccgtctgaaa tgattttggc tttttccaag gcaagaccga gcagccattg
     3001 gtctttttcc aactcgtcct tgaggtaacg ttcctgtttg ccgtatttcg ctttatacaa
     3061 aggcggctgg gcaatgtaga tgtagccgcg ctcgaccagt tcgggcattt ggcggtagaa
     3121 gaaggtcaaa agcaaggtgc ggatatgcgc gccgtccaca tcggcatcgg tcatgataat
     3181 gatgcggtgg taacgcagct tctcggcatt gaattcttct ttgccgatgc ccgcgcccaa
     3241 cgcggtaatc aaagcggcta cttcctgact ggcgagcatt ttttcaaagc gcgctttttc
     3301 gacgttcaaa atcttgccct taagcggcaa aatcgcttgg aatttgcggt cgcgcccctg
     3361 catggcggaa ccgcctgcag aatcgccctc gaccagataa agttctgaca atgcggggtc
     3421 tttttcttgg cagtcggcga gtttaccggg tagccccaag ccgtccatta cgcctttgcg
     3481 gcgggtgatt tcgcgggctt tgcgggcggc ttcgcgcgcg cgggcggcat cgacgatttt
     3541 gccggtgatg attttggctt cgttcgggtt ttcttcaagg aagtcggtca gcgcttggtt
     3601 gatgacttcg ttgacaacgg ggccgatttc gctggaaacc agtttgtctt tggtttggga
     3661 tgagaatttg gggtcgggca gtttgacgga caacacgcag gtcaaacctt cgcgcatatc
     3721 gtcgcctgct gtttccactt tggctttttt ggcgacttcg ttggcttcga tgtagctgtt
     3781 gatggtgcgt gtcatcactt ggcgcaacgc ggtcaagtgc gtaccgccgt cgcgctgagg
     3841 gatgttgttg gtgaagcact gcacgctttc ttggtagctg tcgttccact gcatcgcgca
     3901 ttcgacgctc atgccgtctt tctcgccgaa cgcatagaag attttttcgt gcaacggcgt
     3961 ttttttgcgg ttcatgtatt gcacgaaacc ggccacgccg ccggaaaggg cgaagctttc
     4021 gtgtttgccg tcgcgttcgt cggtcaattc gatgtccacg ccgttgttca ggaaggaaag
     4081 ttcgcgaatg cgtttggcga ggatgtcgaa gctgtattcg acgttgccga aagtttccgt
     4141 gctggcgagg aagcgtacgg tcgtgccttt cttgtcggaa tcgccgacaa ctttcagcgg
     4201 ctcttcggtt tcgccgcgca cgaagcggac gaagtgttct ttgccgtcgc ggtagatggt
     4261 cagcgtaacc cagtcggaca gcgcgttgac gacggacacg cccacgccgt gcaggccgcc
     4321 ggagattttg tagctgttgt tgtcgaactt gccgcctgcg tgcaatacgg tcatgatgac
     4381 ttcggcggcg gagcgtcctt ctttcgggtg gatgccggtg ggcataccgc gcccgttgtc
     4441 ggcgacgctg acggaatggt cggcgtgtat cgttaccgtg attttgtcgc aatgtccggc
     4501 gagtgcttcg tcaatggcgt tgtccaatac ttcgaacacc atgtggtgca gaccgctgcc
     4561 gtcctgcgtg tcgccgatgt acatgccggg gcgtttgcgt accgcttcca agccttcgag
     4621 cacctgaatg ctgtcggcgc cgtattcttc gtgtttttgt tcagtcatat tttttgccgg
     4681 attttgaaaa gattttgcga tgccgccaaa acaagtccgc accttgtaga aaaagcgggc
     4741 gggacggcat atataattgt gtattatagc cgattttgcc gcctaattca gcgttatccg
     4801 catcagtgtg ccgccgggaa aagatgaaac ggtacgtttg cctccggcat caggtcgggg
     4861 attttcccgt aaagtggcaa aagcgttttt ttgccactaa aatctacgcc ctatactttt
     4921 cggacagggg cgcggaaatg gaaatatgga atatgttgga cacttggctc ggtgccgtcc
     4981 cgatacgtgc ggaggcggtc gaatccgtgg cggtggttgc ggctttgctg ctggcgcgcg
     5041 cccttctgtt gaatatccac ttcaaacggc atccggattt cggcatcgaa agcaagcggc
     5101 ggtttttggt tgccagccgc aatataacgc tgcttttggt gctgttttcg ctggcattta
     5161 tctggtcggc gcaaatccaa acgctggctt tgtcgatgtt tgcggtggcg gcggcggtcg
     5221 tcgtggcgac gaaggaactg attatgtgtc tgtcgggcag tattttaagg tctgccaccc
     5281 agcaatactc ggtcggcgac tatatcgaaa tcaacggcct gcgcgggcgc gtggtcgaca
     5341 tcaacctgtt gaacacgctg atgatgcagg tcggtccgaa ccccttggtc ggacagcttg
     5401 cgggaaccac cgtttctttc cccaacagcc tgttgttgag ccaccccgtg cgccgcgaca
     5461 atattttggg cgactatgtc atccatacgg tcgaaatccc cgttcccatc catttggatt
     5521 cggatgaagc cgtatgccgt ctgaaagccg tactcgagcc cttgtgcgcg ccctacatcc
     5581 ccgccatcca acggcatttg gaaaacgtgc aggcggaaaa actgtttatc acgcccgccg
     5641 ccagaccgcg cgttacccgc gtgccgtacg atgacaaggc ataccgcatc atcgtccgct
     5701 tcgcctcccc cgtttcaaag cggctggaaa tccaacaggc ggttatggac gaatttttgc
     5761 gcgtacaata ccgcctgtta aattaccccg ccggctccga aacactttaa ctttccccga
     5821 ccgaccccat ttccggcttc agacggcata ttgccgatat gctgtctgaa gcacaacacg
     5881 caaaggaaac ccatcttatg actgacaacg cactgctcca tttgggcgaa gaaccccgtt
     5941 ttgatcaaat caaaaccgaa gacatcaaac ccgccctgca aaccgccatc gccgaagcgc
     6001 gcgaacaaat cgccgccatc aaagcccaaa cgcacaccgg ctgggcaaac actgtcgaac
     6061 ccctgaccgg catcaccgaa cgcgtcggca ggatttgggg cgtggtgtcg cacctcaact
     6121 ccgtcgccga cacgcccgaa ctgcgcgccg cctacaatga attaatgccc gaaatcaccg
     6181 ccttctttac cgaaatcgga caagatattg ccttatacaa ccgcttcaaa accatcaaaa
     6241 actcccccga atttgcaacg ctttccaccg cacaaaaaac caagctcaac cacgacctgc
     6301 gcgatttcgt cctcagcggc gcggaactgc cgcccgaaca gcaggcagaa ctggcccaac
     6361 tgcaaaccga aggcgcacaa ctctccgcca aattctcgca aaacgtccta gacgcgaccg
     6421 acgcattcgg catttacttt gacgatgccg caccgcttgc cggcattccc gaagacgcgc
     6481 tcgcgatgtt tgccgccgcc gcgcaaagcg aaggcaaaac aggctacaaa atcggcttgc
     6541 agattccaca ctacctcgcc gtcatccaat acgccgacaa ccgcgaactg cgcgaacaaa
     6601 tctaccgcgc ctacgttacc cgcgccagcg aactcgcaaa cgacggcaaa ttcgacaaca
     6661 ccgccaacat cgaccgcacg ctcgcaaacg ccctgcaaac cgccaaactg ctcggcttca
     6721 aaaactacgc cgaattgtcg ctggcaacca aaatggcgga cacgcccgaa caagttttaa
     6781 acttcctgca cgacctcgcc cgccgcgcca aaccctacgc cgaaaaagac ctcgccgaag
     6841 tcaaagcctt cgcccgcgaa agcctgaacc tcgccgattt gcaaccgtgg gacttgggct
     6901 acgccagcga aaaactgcgc gaagccaaat acgcgttcag cgaaaccgaa gtcaaaaaat
     6961 acttccccgt cggcaaagta ttaaacggac tgttcgccca aatcaaaaaa ctctacggca
     7021 tcggatttac cgaaaaaacc gtccccgtct ggcacaaaga cgtgcgctat tttgaattgc
     7081 aacaaaacgg cgaaaccata ggcggcgttt atatggattt gtacgcacgc gaaggcaaac
     7141 gcggcggcgc gtggatgaac gactacaaag gtcgccgccg tttttcagac ggcacgctgc
     7201 aactgcccac cgcctacctc gtctgcaact tcgccccgcc cgtcggcggc agagaagcgc
     7261 gtttgagcca cgacgaaatc ctcatcctct tccacgaaac cggacacggg ctgcaccacc
     7321 tgcttaccca agtggacgaa ctgggcgtat ccggcatcaa cggcgtagaa tgggacgcgg
     7381 tcgaactgcc cagccagttt atggaaaatt tcgtctggga atacgacgtc ttggcacaaa
     7441 tgtccgccca cgaagaaacc ggcgttcccc tgccgaaaga actcttcgac aaaatgctcg
     7501 ccgccaaaaa cttccaacgc ggcatgttcc tcgtccggca aatggagttc gccctctttg
     7561 atatgatgat ttacagcgaa gacgacgaag gccgtctgaa aaactggcaa caggttttag
     7621 acagcgtgcg caaagaagtc gccgtcgtcc gaccgcccga atacaaccgc ttcgccaaca
     7681 gcttcggcca catcttcgcc ggcggctact ccgcaggcta ttacagctac gcatgggcgg
     7741 aagtattgag cgcggacgca tacgccgcct ttgaagaaag cggcgacatt gccgccacag
     7801 gcaaacgctt ctggcaagaa atcctcgccg tcggcggatc gcgcagcgcg gcagaatcct
     7861 tcaaagcctt ccgcggacgc gaaccgagca tagacgcact gctgagccac agcggcttcg
     7921 acaacgcggc ttgatcgatg tgaacaaaaa atgccgtctg aaacaatcaa acagtgtttc
     7981 agacggcatc agcatttgca cggtttcgcc attttcacaa aaacagtaag ccaaaatccc
     8041 gtcattcccg cgcaggcggg aatccagacc ggtcggcacg gaaacttatc gggtaaaaag
     8101 gtttctccgg tcctgaatcc tagattcccg ctttcgcggg aatgacgcgg aaaagttgct
     8161 gtgatttcgg ataaattttc gcaacgttta atttccgttt tacctgataa atgcccgcaa
     8221 tctcaaatcc cgtcattccc caaaaacaaa aaaaatcaaa aacagaaatc
//
LOCUS       DAXHSF010000084         8252 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.084, whole genome shotgun sequence.
ACCESSION   DAXHSF010000084 DAXHSF010000000
VERSION     DAXHSF010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8252
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.084"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(152..1084)
                     /gene="cysK"
                     /locus_tag="ACFM8S_001314"
     CDS             complement(152..1084)
                     /gene="cysK"
                     /locus_tag="ACFM8S_001314"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFC6314381.1"
                     /translation="MKIANSITELIGNTPLVKLNRLTEGLKAEVAVKLEFFNPGSSVK
                     DRIAEAMIEGAEKAGKINKNTVIVEATSGNTGVGLAMVCAARGYKLAITMPESMSKER
                     KMLLRAFGAELILTPAAEGMAGAIAKAKSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
                     EEIWRDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGP
                     HPIQGIGAGFIPTVLNTKIYDSITKVSNEAAFETARAIAEKEGILVGISSGAAVWSAL
                     QLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA"
     gene            complement(1278..1883)
                     /locus_tag="ACFM8S_001315"
     CDS             complement(1278..1883)
                     /locus_tag="ACFM8S_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314382.1"
                     /translation="MILLHLDFLSALLYAAVFLFLIFRAGMLQWFWASIALWLGISVL
                     GAKLMPGIWGMTRAAPLFIPHFYLTLGSIFFFIGHWNRKTDGNGWQADSEHPLLGLFA
                     VSNVSMTLAFVGICALVHYCFSGTVQVFVFAALLKLYALKPVYWFVLQFVLMAVAYVH
                     RCGIDRQPPSTFDGSQLRLGGLTAALMQISVLVLLLSEIGR"
     gene            complement(1880..2731)
                     /gene="dapF"
                     /locus_tag="ACFM8S_001316"
     CDS             complement(1880..2731)
                     /gene="dapF"
                     /locus_tag="ACFM8S_001316"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFC6314383.1"
                     /translation="MNTLKFTKMHGLGNDFMVIDAVSQDFTPEDAPIAAWADRFRGVG
                     FDQLLVVGRSETEGVDFRYRIFNADGSEVGQCGNGARCFARFVADKGLTGKKEICVET
                     ANGVIFPKLSDNGMVTVNMGKPKFMPSEIPFVPESGEGDDACIYGVHLESGIQPVSCV
                     NMGNPHAVIVVDDVECAPVRETGSLIEPHRQFPERVNVGFMQVVGRTAIRLRVFERGV
                     GETQACGTGACAAVVAGIRLGLLDEGKTVEVILPGGTLYIEWACGGDVMMTGPAETVF
                     EGELAYS"
     gene            2868..3164
                     /locus_tag="ACFM8S_001317"
     CDS             2868..3164
                     /locus_tag="ACFM8S_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314384.1"
                     /translation="MFRILLLLFPCAILAACTRNIPANPAVSNCAHLQNRTILYFDAH
                     GKPADGAVKGGFYKIIEIQGANCILIQDFNSTGNALGGSYVPDCRKSSLFPKNI"
     gene            3178..3846
                     /locus_tag="ACFM8S_001318"
     CDS             3178..3846
                     /locus_tag="ACFM8S_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFC6314385.1"
                     /translation="MNHDITFLTLFLLGFFGGTHCIGMCGGLSSAFALQLPPHINRFW
                     LILLLNTGRVSSYTAIGLILGLIGQVGVSLDQTRVLQNILYTAANLLLLFLGLYLSGI
                     SSLAAKIEKIGKPIWRNLNPILNRLLPIKSIPACLAVGILWGWLPCGLVYSASLYALG
                     SGSAATGGLYMLAFALGTLPNLLAIGIFSLQLKKIMQNRYIRLCTGLSVSLWALWKLA
                     VLWL"
     gene            complement(3919..6042)
                     /gene="pnp"
                     /locus_tag="ACFM8S_001319"
     CDS             complement(3919..6042)
                     /gene="pnp"
                     /locus_tag="ACFM8S_001319"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFC6314386.1"
                     /translation="MMFNKHVKTFQYGNQTVTLETGEIARQAAAAVKVSMGDTVVLVA
                     VTTNKEVKEGQDFFPLTVDYLERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRP
                     LFPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYVNG
                     VYVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQVAIN
                     AINEFADEVNPELWDWKAPETNEELVAKVRGIAGETIKEAFKIRQKQARSAKLDEAWN
                     AVKEALITEETDTLAANEIKGIFKHLEADVVRSQILDGQPRIDGRDTRTVRPLNIQTS
                     VLPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEV
                     GRMGAPKRREIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCL
                     SLLSAGVPLKAHVAGIAMGLILEGNKFAVLTDILGDEDHLGDMDFKVAGTTEGVTALQ
                     MDIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKINQDKI
                     REVIGKGGETIRAITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEQITAEVEVGK
                     VYEGTVVKILDNNVGAIVSVMPGKDGLVHISQIAHERVRNVGDYLKVGQVVNVKALEV
                     DDRGRVRLSIKALLDAPAREENAAE"
     gene            complement(6276..7139)
                     /locus_tag="ACFM8S_001320"
     CDS             complement(6276..7139)
                     /locus_tag="ACFM8S_001320"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFC6314387.1"
                     /translation="MSEIGLVKIYSGKVRDLYEIDDKRMLMVASDRLSAFDVILDDPI
                     PSKGEILTQISNFWFKKLAHIMPNHFTGDTVYDVLPENEAKVLEKRAVVAKKLTPVKV
                     EAIVRGYLAGSGWKDYQKTGSVCGIQLPEGMQEAQQLPEVIFTPSTKAAVGDHDENIS
                     FEECERIIGKELAAEVRAKAIQLYTEAAEYAKSRGIIICDTKFEFGLDEEGTLTLMDE
                     VLTPDSSRFWPADQYKVGTNPPSFDKQFVRDWLEQSGWNKKAPAPKVPADVIQKTVEK
                     YQEALTLLTQD"
     gene            7339..8202
                     /gene="rfbD"
                     /locus_tag="ACFM8S_001321"
     CDS             7339..8202
                     /gene="rfbD"
                     /locus_tag="ACFM8S_001321"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFC6314388.1"
                     /translation="MRILLTGSKSQLARCLRDRLPEDWETIATDSASLDITDADAVCN
                     MVKSFQPDAIVNTAAYTAVDKAEGDAAAAFAVNASAVYNLALAAHRAHARFIHISTDY
                     IFDGKGKRPYQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFVR
                     TMLNLARERSPLSAVHNQIGCPTYAGDLSAAIIRLLQQSNPVRGIYHYVGGKSVSWYE
                     FAQHIFQAASQQQTSFPVPELTAVSDKEYPTAAPRPAYSILDCRKIENDFSIKPSDWQ
                     KALAQVVSKLL"
BASE COUNT         1983 a         2384 c         1904 g         1981 t
ORIGIN      
        1 aaaatatttc cctcaagcaa tgcggcggca ccaatgcctg attaaaacac aaccggcgtt
       61 ttcggcatat atgcaaaaac gcattttccg gcacggcgtt acaccgatac cgaaaaatgc
      121 gtctgaatgt ttcgacaatc cgattaaagc attatgccaa atctgcaaaa agtggcgtag
      181 agagatagcg ttcgccataa gaaggcagca gcacgactat cagcttgcct tcgttttcag
      241 gctgtttggc aagctgcaac gcgctccaaa ccgccgcacc ggaagaaata cccaccaaaa
      301 tgccttcttt ttccgctatt gcgcgggcgg tttcaaaagc cgcttcgttc gacactttgg
      361 taatgctgtc gtaaattttg gtattcaaaa cggtcggaat aaagcctgcg ccgatgcctt
      421 gaatcgggtg cgggcctttt tcgccgccgc tcaatacggg tgaagcttca ggctcgacgg
      481 caaccacttt aacttcgggt ttgtattttt tcaacacttc gcccacgccg gtaatcgtac
      541 cgcccgtgcc gacgccggca acgaagacat cgactttgcc gtccgtatcc cgccaaattt
      601 cctcggcggt tgttttgcgg tggacttcgg ggtttgcctc attgtcgaac tggcgcggca
      661 taaaataagt gtcgggatgc gcgtccacca aggatttcgc tttggcaatc gcgcccgcca
      721 taccttcggc ggcaggggtc agaatcagct ccgcaccaaa cgcgcgcaac agcattttgc
      781 gctccttact catgctttcc ggcatggtaa tcgccagctt gtagccgcgt gcggcacata
      841 ccattgccaa accgacaccc gtattgccgc tggttgcttc gacaatgacg gtgtttttgt
      901 tgattttgcc cgctttttcg gcaccctcga tcattgcttc ggcaatgcgg tctttgacgc
      961 tgctgcccgg attgaaaaat tccagtttca cggcaacctc cgccttcaaa ccttcggtca
     1021 gacggttcag tttgaccaaa ggcgtgttgc cgattagttc ggtgatgctg tttgcaattt
     1081 tcatcttctg ctcctgaata ataaggtttc gacagtaagg cgcaatcccc ataggcatcc
     1141 tgtccgattg caggggattt gcgccgaaac ataaacaaat ttcggatatg gctttatcct
     1201 aaacctatgc cgcctaaaaa accaatatcc aaaatttctt tctttataac tttttgttcc
     1261 gatacggccg caccgtatta tcttccaatt tctgaaagca gcagcaccag taccgagatc
     1321 tgcatcaacg ctgccgtcaa cccgccgagt cgcagctgcg agccgtcgaa cgttgacggc
     1381 ggctgccggt ctataccgca gcggtggaca taggcaaccg ccatcagcac aaactgcaac
     1441 acgaaccaat aaaccggctt cagcgcataa agtttgagca gtgccgcaaa cacaaacact
     1501 tgaaccgttc ccgaaaagca ataatgcacc aacgcacata ttccgacaaa agcaagcgtc
     1561 atcgatacat tactgacggc aaaaagcccg agcagcggat gttcggagtc tgcctgccat
     1621 ccgtttccat ctgttttccg gttccaatgc ccgatgaaaa aaaatatgct gcccaaagtc
     1681 aggtaaaaat gggggatgaa caagggcgcg gcgcgggtca ttccccatat gccgggcatc
     1741 agctttgccc ccaaaaccga tatgccgagc cacagcgcaa tactcgccca aaaccattgc
     1801 aacattcctg cgcggaatat cagaaacaga aaaaccgccg catacagtaa ggcagacaaa
     1861 aaatccaaat gcagcaaaat catgaatacg ccaactcgcc ttcaaacacg gtttccgcag
     1921 ggccggtcat catcacatcg ccgccgcagg cccattcgat atataaagtt ccgcccggca
     1981 aaatcacctc taccgttttc ccttcatcca acaaccccag acggataccc gccaccacag
     2041 ccgcacacgc gcccgtaccg cacgcttggg tttcgcccac gccgcgctcg aacacgcgca
     2101 aacgaatcgc ggttcggccg acaacctgca taaagccgac attgacgcgt tcgggaaact
     2161 gcctgtgcgg ttcgataagc gaaccggttt cgcgcaccgg cgcgcattcc acgtcatcga
     2221 ccacaatcac cgcatgggga ttgcccatat tgacgcagct gacaggctga atgccggatt
     2281 cgagatgcac cccgtaaata caggcatcat ccccctcgcc cgattcgggg acaaacggta
     2341 tttcagacgg cataaacttc ggtttgccca tattgaccgt aaccataccg ttatcggaca
     2401 atttcggaaa aataacgcca tttgccgttt caacacaaat ttctttctta ccggtcaaac
     2461 ccttgtctgc aacaaaacgg gcaaaacaac gcgctccgtt gccgcattgc ccgacctcgc
     2521 tgccgtcggc attgaaaata cggtaacgga aatccacgcc ttcggtttcc gaacgcccga
     2581 ccaccaaaag ctggtcgaag cccacgcccc ggaagcggtc cgcccatgcc gcaatcggcg
     2641 cgtcctcggg ggtaaaatcc tgactgaccg cgtcaatcac cataaaatcg ttgcccaaac
     2701 cgtgcatttt ggtaaatttc aaagtgttca tattgttccg aaatcccgta tcgtctgttg
     2761 cttccgaatc cgaaatcccc cgcaccgaag ggaaatgctt ccatctcccg ccgcctgagg
     2821 attaaaatca gccattatac tgtttgttac cccttacaag aaacaccatg ttccgcatat
     2881 tactgttgct tttcccttgt gcaatccttg ccgcctgcac ccgaaacata ccggcaaacc
     2941 cggccgtttc aaattgcgcg catcttcaaa accgcaccat cctgtatttt gacgcacacg
     3001 gcaaacccgc cgacggcgca gtaaagggcg gattttacaa aattatcgaa atacagggtg
     3061 caaactgcat ccttattcaa gactttaaca gcacgggcaa tgccctcggc ggatcctatg
     3121 tgccggactg ccgcaaatca tcccttttcc cgaaaaatat ttgacttcaa actgcctatg
     3181 aaccacgaca tcactttcct caccctgttc ctactcggtt tcttcggcgg aacgcactgc
     3241 atcggtatgt gcggcggatt aagcagcgcg tttgcgctcc aactcccccc gcatatcaac
     3301 cgcttttggc tgatcctgct gcttaacaca ggacgggtaa gcagctatac ggcaatcggc
     3361 ctgatactcg gattaatcgg acaggtcggc gtttcactcg accaaacccg cgtcctgcag
     3421 aatattttat acacggccgc caacctcctg ctgctctttt tgggcttata cttgagcggt
     3481 atttcttcct tggcggcaaa aatcgagaaa atcggcaaac cgatatggcg gaacctgaac
     3541 ccgatactca accggctgtt acccataaaa tccatacccg cctgccttgc ggtcggaata
     3601 ttatggggct ggctgccgtg cggactggtt tacagcgcgt cgctttacgc gctgggaagc
     3661 ggtagtgcgg caacgggcgg gttatatatg cttgcctttg cactgggtac gctgcccaat
     3721 cttttggcaa tcggcatttt ttccctgcaa ctgaaaaaaa tcatgcaaaa ccgatatatc
     3781 cgcctgtgta cgggattatc cgtatcatta tgggcattat ggaaacttgc cgtcctgtgg
     3841 ctgtaagccc cattcattcc cgatacccgt aaaaaatacc gtctgaaacc tgttcagacg
     3901 gcactttcac cctaagcgtt actcggcggc attttcctca cgggcaggcg cgtccagcag
     3961 ggctttgatg gacagacgga cacggcctct gtcgtccact tccaatgctt tcacgttcac
     4021 cacctgaccg actttcaggt agtcgccgac attgcgtacg cgctcgtggg cgatttggct
     4081 gatgtgtacc aaaccgtctt tgcccggcat cacgctgaca atcgcgccga cgttgttgtc
     4141 gaggattttc accacagtgc cttcatacac tttgcccact tccacttcgg cagtaatctg
     4201 ctcgatgcgt tttttcgccg catcgccggc ttcttgagtg gtcgcggcaa tggtaatcgt
     4261 accgtcttcg gcaatattga tttccgtacc ggtttcagcg gtaatcgcac ggatggtttc
     4321 accgccctta ccgataactt cgcggatttt gtcttggttg attttcatcg taaacaaacg
     4381 cggcgcgtgt gcggacaact cttgcgggcc cgcaacggcg gctttcatct gatccaagat
     4441 gtgcagacgc gcttctttgg cctgtgccaa agcgatttgc ataatttctt tggtaatgcc
     4501 ttggattttg atgtccattt gcagcgcggt aacgccttcg gtcgtaccgg ccactttaaa
     4561 gtccatatcg cccaagtggt cttcgtcgcc caaaatgtcg gtcaggacgg caaatttgtt
     4621 gccttccaga atcagaccca tcgcgatacc ggcaacgtgt gctttcaaag gcacgccggc
     4681 agacaacagg ctcaggcagc cgccgcagac ggaagccata gaggaagagc cgttggattc
     4741 ggtaatttcg gagaccacgc gcatggtgta gctgaaatct tcaggtttcg gcaatacggc
     4801 caacaatgca cgtttagcca aacggccgtg accgatttca cggcgtttcg gtgcgcccat
     4861 gcggcccact tcgccggtag agtacggcgg aaagttgtag tgcagcataa agcggtcggt
     4921 gtattcgccg gacagcgcgt cgatgatttg ctcgtcgcgc gaagtaccca aagttgcaac
     4981 ggccaaagct tgggtttcgc cacgggtaaa caatgcagaa ccgtgcgtgc gcggcaatac
     5041 gctggtttgg atgttcagcg gacggacggt gcgggtgtcg cggccgtcga tgcgcggttg
     5101 gccatccaaa atttggctgc ggacgacatc ggcttccaag tgtttgaaaa tgcctttgat
     5161 ttcgttggct gccaaagtgt cggtttcttc ggtaatcaac gcttctttca cggcattcca
     5221 agcttcgtcc aacttggcag aacgcgcttg tttttgacgg attttgaacg cttctttaat
     5281 ggtttcgccg gcaatcccgc ggactttggc aaccagttcc tcattggttt caggtgcttt
     5341 ccaatcccaa agttccggat tgacttcgtc ggcaaattca ttgattgcat tgatggcaac
     5401 ctgcatttga tcgtggccgt aaaccaccgc gcccagcatc acgtcttcgg gcaggatttt
     5461 ggcttcggat tccaccatca acacggcttt tgaagtaccg gcgaccacca agtccaattg
     5521 cgatttagcc aattcggctt tagtcgggtt caaaacgtac acgccgttta cataacccac
     5581 gcgtgccgca ccaatcggac cggcaaacgg tacgccgctc aacaccagcg cggcagatgc
     5641 gcccaacatt gcaggaatgt cggaatcgat ttccggatcg acagacacca ccatcgctac
     5701 gatttggatg tcgtggtaga aaccttcagg gaataaagga cgaatcggac ggtcgatcag
     5761 acggctggtc aggatttctt tttcgctttg tttgccttcg cgtttgaaga agccgccggg
     5821 gattttgcct gcggcgtaag tacgctcgag gtaatcgacg gtcaggggga agaagtcttg
     5881 accttctttc acttctttat tggtggtaac ggcaaccaaa acgacggtgt cgcccataga
     5941 tactttaacg gcagcggcgg cttggcgggc gatttcgccg gtttccaaag taacggtttg
     6001 attaccgtat tggaaggtct taacgtgttt attgaacatc attgttcctt tcaaaatacc
     6061 gcactgctaa aacactaata atgcacacta aaatccgaat gtgcatagtt agggtttcag
     6121 accgtgcggc aggttataaa caagctttca gacggccttt cagtcgctga aaacaactct
     6181 cgattataaa ggcaaccatc tttaaaatcc agtattgata caataaaaag gccgtctgaa
     6241 acatatctct ttcagacggc cttcaaactt aaaaattaat cctgagtcag caaagtcaat
     6301 gcttcctgat atttttcgac tgttttctga attacatcgg caggcacttt aggcgcaggc
     6361 gcttttttat tccaaccgct ttgctccagc cagtcgcgga cgaattgttt gtcaaaagac
     6421 ggcggattgg tgccgacttt gtattggtcg gcaggccaaa aacggctcga atcgggagtc
     6481 aatacctcat ccatcaacgt cagcgtacct tcttcatcca aaccgaattc aaatttggta
     6541 tcgcaaatga tgatgccgcg cgatttggca tattccgccg cttctgtata aagctgaatc
     6601 gctttggcgc gcacttccgc cgccaattct tttccgataa tgcgttcgca ttcttcaaag
     6661 ctgatgtttt catcgtgatc gccgactgcg gctttggttg agggcgtaaa aatcacttca
     6721 ggcagttgtt gcgcttcctg cataccttca ggcagttgga taccgcaaac cgagccggtt
     6781 ttttgataat ctttccaacc gctgcctgcc agataaccgc gcacaatcgc ctcaactttc
     6841 accggagtga gctttttagc cacgacggcg cgtttttcta aaactttggc ttcgttctca
     6901 ggcaaaacat cgtacacggt atcgccggta aaatggttgg gcataatatg cgccagtttt
     6961 ttaaaccaaa aattggaaat ctgcgtcaga atctcccctt tgctcggaat cgggtcgtcc
     7021 aaaatcacat caaacgcgga caggcggtcg gaagcgacca tcagcatacg tttatcgtcg
     7081 atttcatata aatcgcgcac ttttccagaa tagatcttta ccaaaccaat ctcactcatt
     7141 tcgcaccccc ctgaaaatat cttgaaaata ccgacccgac acccgacagg tttgaatcgc
     7201 aaaccgatat tctagccgaa gtcggcgcaa aacaataccc atggcacaaa aagccaaccc
     7261 gtcaaccgtc ggcaaagttt tggcactata ataccgacag caagtcctac aatacacttt
     7321 taccaaagga aatacctcat gagaatccta ttgacaggct cgaaaagcca actggcacgc
     7381 tgcctgcgcg accgtcttcc ggaagactgg gaaaccattg cgacggattc cgcatcccta
     7441 gacattaccg atgccgatgc cgtctgcaac atggtcaaaa gtttccaacc cgacgccatt
     7501 gtcaacacgg ctgcctatac tgccgtcgac aaagcggaag gcgatgcggc agcggcattt
     7561 gccgtcaatg cttccgccgt ttacaacctt gccttggcag cacatcgcgc ccatgcccga
     7621 ttcatccaca tctcaaccga ctatatcttt gacggaaaag ggaaaagacc ctaccaggaa
     7681 agcgacttta ccaacccttc caatgtatac ggacaatcca aaaccgccgg cgaactgctc
     7741 gcactgtctg ccaatcccga cagccttatc ctgcggactt cttggctgtt tagcgaatac
     7801 ggggacaact ttgtccgcac catgctgaac cttgcgcggg aacgttcccc gctgtccgcc
     7861 gtccacaacc aaatcggctg cccgacctat gccggcgact tgtccgccgc catcatccgc
     7921 ctgttgcagc agtccaatcc cgttcgcggc atttaccact acgtcggcgg caaatccgta
     7981 tcctggtacg aatttgccca acatattttc caagcggcat cgcaacagca gacatccttc
     8041 cccgttcccg aattgactgc cgtttcagac aaggaatatc cgaccgccgc ccccaggccc
     8101 gcatacagca ttttggactg ccgcaaaatc gaaaacgact tcagcatcaa accgtcagac
     8161 tggcaaaaag cccttgcaca ggtcgtttcc aagctgctct gatgccgccc gcctctccgt
     8221 ttccgccgtc aagcaccgcc ttggcggttt tc
//
LOCUS       DAXHSF010000085         8245 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.085, whole genome shotgun sequence.
ACCESSION   DAXHSF010000085 DAXHSF010000000
VERSION     DAXHSF010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8245
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.085"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(636..3425)
                     /gene="ileS"
                     /locus_tag="ACFM8S_001322"
     CDS             complement(636..3425)
                     /gene="ileS"
                     /locus_tag="ACFM8S_001322"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFC6314389.1"
                     /translation="MTDYSKTVNLLESPFPMRGNLAKREPAWLKSWYEQKRYQKLREI
                     AKGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIRSKTQAGFDAPYVPGWDCHGLPI
                     EVMVEKLHGKDMPKARFRELCREYAAEQVARQKKDFIRLGVLGDWDKPYLTMDFKTEA
                     DTVRMLGEIYKSGYLYRGAKPVQFCLDCGSSLAEAEVEYKDKVSPAIDVGYPFKDTAA
                     LAAAFGLAGIEGKAFAVIWTTTPWTLPASQAVSAGADVVYQLIDTPKGKLVLAKDLAE
                     DALKRYGFSDGIAILAETTGDKLENLHMNHPFLERDIPMLNGDHVTTDAGTGLVHTAP
                     AHGLEDYAVCNKYGIELYNPVNAEGKYISETPRVAGMSVWEANPVILQWLEEIGNLLA
                     SSKIEHSYAHCWRHKTPLIYRATGQWFVGMDKAGADGKTLRDKAIKAVDDTEFFPSWG
                     RARLEAMIEGRPDWVVSRQRYWGTPMTFFVHKETGELHPNSAELLEKVAQRIEEKGIE
                     AWFSLDKGELLSAEDCEHYDKLSDTMDVWFDSGSTHYSVVKQREELDWPADLYLEGSD
                     QHRGWFQSSMLTGCASSMGRAPYKQLLTHGFVVDQNGRKMSKSIGNVVAPQEVYNEFG
                     ADILRLWAASTDYSGELAISKEILKRVTESYRRIRNTLSFLFANLSDFNPIEDAVQQA
                     DMVEIDRYAVVLARRLQERLAGDYYPRYAFHFAVKDIVSFCSEDLGAFYLDILKDRLY
                     TTKADSRARRSAQTALYHITRSLVLLIAPILCFTGEEAWDIIGGGEEDSVLFHTWHEF
                     PTINEKAEAELVKKWTAIREAREAVTAAIEPLRADKTVGSSLQAEAEITAPEEMAGYL
                     NALGEELRFALLVSKAEVKVGSELAVAAKASDGEKCERCWHYTRDVGAVAGYETVCKR
                     CAENVGREGETRHYA"
     gene            complement(3566..4486)
                     /gene="ribF"
                     /locus_tag="ACFM8S_001323"
     CDS             complement(3566..4486)
                     /gene="ribF"
                     /locus_tag="ACFM8S_001323"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFC6314390.1"
                     /translation="MRIRLGRHNAPDFPHGAAVTIGNFDGVHLGHKHILQKLRLEADA
                     RGLPVVAVIFEPQPKEFFAALAGKTPPCRISPLRTKLELLEGTGCVDAVWVLRFDQNF
                     SEISAQGFIDRLLRQTLNTRYLLVGDDFRFGAGREGCFELLAQQPDMQTERTPSVIVE
                     DIRTSSTAVRQALSDGNLAYAKKLLGHDYVLSGRVVHGRKLGRTLNAPTANIRLPRHR
                     YALGGVFVVEADGAFGTRRGVASFGFNPTVDSDCFQKLEVHLFDFQGDLYGQRLNVRF
                     LHKLRDEEKFDGMEELKRQIEADMEAAKCW"
     gene            complement(5013..6308)
                     /gene="tyrS"
                     /locus_tag="ACFM8S_001324"
     CDS             complement(5013..6308)
                     /gene="tyrS"
                     /locus_tag="ACFM8S_001324"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFC6314391.1"
                     /translation="MSVIQDLQSRGLIAQTTDIEALDALLNEQKISLYCGFDPTADSL
                     HIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVESIR
                     SQLTPFLSFEGGNAAIMANNADWFGKMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
                     GAGISFTEFAYSLLQGYDFAELNKRHGAVLEIGGSDQWGNITAGIDLTRRLNQKQVFG
                     LTLPLVTKSDGTKFGKTEGGAVWLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSI
                     EEIDAVEAKDQASGSKPEAQRILAEEMTRLIHGEEALAAAQRISESLFAEDQSSLTES
                     DFEQLALDGLPAFEVSDGINVVEALVKTGLASSNKEARGFVNSKAVLLNGKPAEANNP
                     NHAAERPDDACLLTDEHKRFGKYTILRRGKRNHALLVWK"
     gene            complement(6371..8239)
                     /locus_tag="ACFM8S_001325"
     CDS             complement(6371..8239)
                     /locus_tag="ACFM8S_001325"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFC6314392.1"
                     /translation="MSQALPYRPDIDTLRAAAVLSVIVFHIEKNWLPGGFLGVDIFFV
                     ISGFLMTMILHREMSGGGRFSLKAFYIRRIKRILPAFFAVLAATLTGGFFLFTKDDFF
                     LLWKSALTALGFASNLYFARGKDYFDPAQEEKPLLHIWSLSVEEQFYFVFPILLLLVA
                     RKSPRVQFGFLAALCALSLAASFMPSAFDKYYLPHLRACEMLVGSLTAVWMRCRQPAV
                     GRRCAAAGALFAVCILSTCLFSYSEQTAYFPGPAALIPCLAVAALIYFNHYEHPLKKF
                     FQWKITVAAGLISYSLYLWHWPILAFMRYIGPDNLPPYSPAAAIVLTLAFSLISYHCI
                     EKPFKKWKGSFAQSVLWIYALPMLILGAGSFFAMRLPFMAQYDRLGLTRSNTSCHNNT
                     GKQCLWGDTEKQPELLVLGDSHADHYKTFFDAVGKKEKWSATMVSADACAYVEGYASR
                     VFQNWAACRAVYRYAEEHLPLYSKVVLAMRWGSQMPENSRSLAYDAGFFQKFDRMLHK
                     LSSEKQAVYLMADNLASSYNVQRAYILSSRIPGYRQTLRPDDESTLKANARIRELAAK
                     YPNVYIIDAAAYIPANFQIGGLPVYSDKDHINPYGGTELAKRFSEKQRFLDTRHNH"
BASE COUNT         1842 a         2138 c         2342 g         1923 t
ORIGIN      
        1 cgtgggaatg acggttcagt tgcattccga caacaccgca atctcgaaat ccgtcattcc
       61 cgataacagc gcaatcttga aacccgtcat tcccgcgcag gcgggaatct agacccctga
      121 cgcggcggga atctatcgga aatgactgaa accccaagat tctagattcc cactttcgtg
      181 ggaatggcgg ttcagttgca ttccgacaac accgtaatct tgaaatccgt cattcccgat
      241 aacagcgcaa tcttgaaacc cgtcattccc gcgcaggcgg gaatccagac ctccgacgcg
      301 gcgggaatct atcggaaatg actgaaaccc cgagattcta gattcccact ttcgtgggaa
      361 tgacggttca gttgcgttcc gacaacaccg taatctcgaa atccgtcatt cccgcacagg
      421 cgggaatcca gacccacgac gcggcgggaa tctatcggaa atgactgaaa cctcgagatt
      481 ctagattccc acttccgtgg gaatgacggt tcagttgcat tccgacaaca ccgcaatctt
      541 gaaacccctc cgccgttata aagacaaatt gcggcacaaa aaatgccgtc tgaaatgctg
      601 ttcggcggtt tcagacggca tttgctcaaa ctttatcagg cgtaatggcg cgtttcgcct
      661 tctctgccga cattctctgc acagcgtttg cagacggttt catagcctgc aaccgcgccc
      721 acatcgcggg tgtagtgcca gcagcgttcg catttttcac catcactggc tttagcggca
      781 acggcaagtt cgctgcctac tttcacttct gctttagaca ccagcaaggc aaagcgcaat
      841 tcttcgccca aagcgttcag gtagccggcc atttcttccg gcgcagtgat ttcggcttcg
      901 gcttgcaagg acgaaccgac ggttttgtcg gcgcgcaaag gctcgatggc ggcggttacc
      961 gcttcgcggg cttcgcggat tgccgtccat tttttcacca gttcggcttc ggctttttcg
     1021 ttgatggtcg ggaactcgtg ccaggtgtgg aagaggacgc tgtcttcttc gccgccgccg
     1081 atgatgtccc acgcttcttc gccggtaaag cacaaaatcg gcgcaatcaa gagaaccaaa
     1141 ctgcgcgtga tgtggtacag ggcggtttgc gcgctgcggc gggcgcggct gtcggctttg
     1201 gtggtgtaga ggcggtcttt caggatatcg aggtagaacg cgcccaagtc ttccgagcag
     1261 aaagaaacaa tgtctttcac ggcaaaatgg aaggcataac gcggatagta atcgcctgcc
     1321 agacgctctt gcagccgacg cgccaatacc acggcgtagc ggtcgatttc caccatgtct
     1381 gcctgttgca cggcatcttc aataggattg aagtcgctga ggttggcaaa caggaagctc
     1441 aaggtgttgc ggatacggcg gtagctttcg gttacgcgct tgaggatttc tttggaaatc
     1501 gccaattcgc cgctgtaatc ggtggatgcc gcccacaggc gcaggatgtc cgcgccgaac
     1561 tcgttataca cttcttgcgg cgcgacgacg ttgccgatgg atttcgacat tttgcgcccg
     1621 ttttgatcca ccacgaaacc atgggtcagc agctgtttgt acggcgcgcg acccattgat
     1681 gaggcgcagc cggtcagcat ggacgactga aaccagcccc ggtgttgatc gctgccttcg
     1741 aggtacaagt cagcaggcca gtccaattct tcgcgctgtt tcacaacgga ataatgggtc
     1801 gaaccggagt cgaaccatac gtccatcgtg tcggagagtt tgtcgtaatg ttcgcaatct
     1861 tccgcgctca acaattcgcc cttatcgagg gagaaccaag cctcgatgcc tttttcttcg
     1921 atgcgttgcg cgactttttc caaaagttcg gcagagttcg gatgcagctc gcccgtttct
     1981 ttgtgaacaa agaaagtcat cggcgtgccc caatagcgtt ggcgcgaaac cacccagtcg
     2041 gggcggcctt caatcatggc ttccaaacgc gcacgacccc atgatgggaa aaattcagtg
     2101 tcatcaacgg ctttgatggc tttatcgcgc agggttttac catcggcacc tgctttatcc
     2161 ataccgacaa accattggcc ggtcgcacgg taaatcaacg gcgttttgtg acgccagcag
     2221 tgcgcgtagc tgtgttcgat tttgctgctt gccaagaggt tgccgatttc ttccaaccat
     2281 tgcaggataa cgggattggc ctcccaaacg ctcatgccgg caacgcgcgg cgtttcactg
     2341 atgtatttgc cttcggcgtt gacggggttg taaagctcga tgccgtattt attgcagacg
     2401 gcgtagtctt ccaaaccatg agcaggcgca gtatgcacca aaccggtacc ggcgtcggtg
     2461 gtaacgtggt cgccgttgag catgggaata tcgcgttcga ggaacggatg attcatgtgc
     2521 aggttttcca gcttgtcgcc ggtggtttcg gcaagaatag caatgccgtc tgaaaaaccg
     2581 taacgtttga gcgcgtcttc tgccaaatct ttcgccagca ccaatttgcc tttgggcgta
     2641 tcgattaatt gatacaccac gtccgcaccg gcagacacgg cttggctcgc tggcagcgtc
     2701 caaggcgtgg tcgtccagat gacggcaaac gctttgccct cgatgcctgc caaaccgaat
     2761 gcagcggcaa gcgcagcggt gtctttaaac ggatagccca cgtcaatcgc aggcgatact
     2821 ttgtctttgt attccacttc ggcttcggcc aatgaagagc cgcagtccaa gcagaactga
     2881 accggtttcg cgccgcggta gagatagccg gatttgtaga tttcgccgag catacgcacg
     2941 gtatcggctt cggttttaaa atccatggtc aggtagggct tgtcccagtc gcccaacacg
     3001 cccagacgga taaagtcttt tttctggcgg gcaacttgtt cggcggcata ttcgcggcac
     3061 aattcgcgga aacgcgcttt gggcatgtct ttgccgtgca gcttttctac catcacttcg
     3121 ataggcaaac cgtgacagtc ccaacccggc acataaggcg cgtcaaaacc ggcttgggtt
     3181 ttgctgcgga taatgatgtc tttgagaatt ttgttgacgg cgtgaccgat gtggatgtcg
     3241 ccgttggcat acggcgggcc gtcgtgcagg atgaacttcg gacgaccttt ggcgatttcg
     3301 cgcagttttt ggtagcgttt ttgctcgtac cagcttttca gccatgcagg ctcgcgcttg
     3361 gcaagattgc cgcgcatcgg aaacgggctt tcgagcaggt ttacggtttt gctgtaatcg
     3421 gtcatttttt aatctctatt gttacaatat ttcggttcag acggcattgc gcctcaaaca
     3481 gtatttcaca acgggaaacc cctatgccgt ctgaacagtt aaaaggttga ttgtagccca
     3541 atcggatggt ttgtataagg tttttctacc aacactttgc ggcttccata tcggcttcaa
     3601 tctgcctttt cagttcttcc ataccgtcaa acttttcctc atcgcgcagt ttgtgcagga
     3661 agcggacgtt caaccgttgt ccgtacaaat cgccttgaaa gtcgaacagg tggacttcca
     3721 gcttttgaaa acagtcgcta tcaacggtgg gattgaagcc gaagctcgcc acgccgcgcc
     3781 gcgtgccgaa cgcgccgtcc gcttcgacga cgaacacgcc gccgagtgca taacggtggc
     3841 ggggcaggcg gatgttggca gtcggggcgt ttaaggtgcg tccgagtttt ctgccgtgca
     3901 ccaccctgcc gctcaagacg tagtcgtgtc ccaaaagttt tttcgcatag gcaaggttgc
     3961 cgtctgaaag ggcttgtcgc acggcggtac tgctggtgcg gatgtcttcg acgatgacgg
     4021 aaggcgtacg ctcggtctgc atatcgggct gttgtgccaa aagttcaaaa cagccttccc
     4081 gccccgcacc gaaacggaaa tcgtcgccga cgagcaaata acgcgtattc aaggtttgac
     4141 gcagcaggcg gtcgataaac ccttgcgcgg atatttcgga aaaattttga tcgaaacgca
     4201 aaacccagac ggcatcgacg cagcccgtcc cttccagcaa ttccagtttg gtacgcaggg
     4261 ggctgatgcg gcagggcggg gttttgcctg cgagggcggc aaaaaattct ttgggttgag
     4321 gttcgaagat gacggcaacg acgggcagtc cgcgcgcgtc ggcttcgagg cggagttttt
     4381 ggaggatgtg tttgtgtccg aggtgtacgc cgtcgaaatt gcctatggtt acggcggccc
     4441 cgtgcggaaa gtcgggcgcg ttgtgccgcc ccagcctgat tctcattgtt atgttcggga
     4501 gtgtggtcaa acaggcggtc attgtaaacg gtattgcggt ttatagacag tgtgccgccc
     4561 gttacgcccg cccgacgcgg gaaaagtagg caaatttccc gcagccgaac gcgccaaacg
     4621 cataaagcgc gcagcaggcg cggtgctatg tgttgaaaca tcgccccaaa cctgatgcaa
     4681 catcagtgaa aatcgttctt ttttaaccag gtaaaccgag acaaacaacc ctccgccgtc
     4741 attcccgcgc aggcgggaat ccggacctgt ccgcacagaa acttatcgga taaaaacagt
     4801 tgctcaaacc ccgagattct agattcccac tttcgtggga atgacggttc agttgcgttc
     4861 cgacaacacc gtaatcttga aactcgtcat tcccgctcag gcgggaatct agaacgtgga
     4921 atctaagaaa ccgttttgcc cgataagttt ccgtgcggac aggtccggat tcccgcctgc
     4981 gcgggaatga cggcatttct gcggcaatcg gattatttcc aaaccaaaag cgcgtgattg
     5041 cgtttgccgc gccgaaggat ggtgtatttg ccgaaacgtt tgtgttcgtc ggtcagcagg
     5101 caggcatcgt cggggcgttc ggcggcgtgg ttggggttgt tggcttcggc aggtttgccg
     5161 ttgagcaaaa ccgctttgct gttcacaaag ccgcgcgctt ctttattgga ggatgccaaa
     5221 ccggttttga ccaaggcttc gacgacattg atgccgtctg aaacttcaaa tgcaggcagg
     5281 ccgtcgaggg cgagctgctc gaagtcgctt tcggtcaggc tgctttggtc ttcggcaaac
     5341 aggctttcgg aaatgcgctg cgcggcggca agggcttctt cgccgtgaat caggcgggtc
     5401 atttcttcgg cgaggatgcg ttgcgcttcg ggcttgctgc cgcttgcctg gtctttggct
     5461 tcgacggcat cgatttcttc gatggacagg aaggtaaagt atttcaggaa tttatacaca
     5521 tcggcatcgg cgactttcag ccagaattgg tagaactgat agggcgaggt ttttttcgcg
     5581 ttcagccata ctgcgccgcc ttcggttttg ccgaatttgg tgccgtctga tttggtaacc
     5641 aaaggcaggg tcaggccgaa cacttgtttt tgattcaggc ggcgggtcag gtcgataccg
     5701 gcggtgatgt tgccccattg gtcggagccg ccgatttcca aaaccgcgcc gtggcgtttg
     5761 ttcaactcgg cgaagtcgta gccttgcagc agggagtagg cgaactcggt gaaggaaatg
     5821 cctgcgccgt cgcggtcaat gcgctgtttg acggattctt tgttcagcat ggcgttgacg
     5881 gagaaatgct tgccgatgtc gcgcaggaag tcgaggcagt tcattttgcc gaaccagtcg
     5941 gcgttgttcg ccataatggc ggcgtttccg ccttcaaagc tcaagaaagg ggttaattgg
     6001 ctgcggatgc tttccaccca gccggcaacg gtttcggcgg aattcaagct gcgttcggcg
     6061 gctttgaagc tggggtcgcc gatcataccg gtcgcaccgc ccaccagtgc aatcggcgta
     6121 tgccccgcct gttggaagcg gcgcaatgcc aatacgggca gcaggtgtcc gatgtgcagg
     6181 ctgtcggcgg tcgggtcgaa gccgcaataa agggaaattt tttgttcgtt caacaaagcg
     6241 tctaaggctt cgatgtcggt ggtttgcgcg ataaggccgc gcgattgcag gtcttggatg
     6301 acgctcatcg gtctctttca aaaaaaatta gcggttttgc aaaccgccga ttgtaacaaa
     6361 tttaagcgaa tcaatggtta tggcgcgtat cgagaaaccg ttgtttttcg gaaaaacgct
     6421 tcgccaattc cgtgccgccg taagggttga tgtggtcttt gtccgagtaa accggcaatc
     6481 cgccgatttg gaaatttgcg gggatatagg cggcggcatc aataatatag acgttggggt
     6541 atttggctgc caattccctg atgcgtgcat tggctttcag agtgctttcg tcgtccgggc
     6601 gcagggtttg gcggtaaccc ggtatgcgtg aagacaagat ataggcgcgt tgtacgttgt
     6661 aagacgaggc aaggttgtcc gccatcaggt aaacggcttg tttttcggat gagagtttgt
     6721 gcagcatacg gtcgaatttt tggaaaaaac cggcatcgta ggcaagggag cggctgtttt
     6781 cgggcatttg gctgccccag cgcatcgcca aaaccacttt tgaatacagg ggcaggtgtt
     6841 cttcggcata gcggtaaacg gcgcggcagg cggcccagtt ttggaacaca cgggacgcgt
     6901 agccttccac ataggcgcaa gcgtcggcgg aaaccatagt ggcggaccat ttttcttttt
     6961 tgcccacggc atcgaagaat gttttgtaat ggtcggcgtg ggaatcgccc aaaaccagca
     7021 gttccggctg tttttccgta tccccccata ggcattgttt gccggtattg ttgtggcagg
     7081 aggtgttgga acgcgtcagc cccaagcggt cgtattgcgc cataaacggc agcctcatcg
     7141 cgaaaaacga gcccgccccc aaaatgagca taggcaaggc ataaatccat aaaacggatt
     7201 gtgcgaacga acctttccat tttttaaacg gcttttcgat gcagtgataa gaaatcaggg
     7261 aaaacgctag agtcaggacg atcgctgccg ccggcgaata aggcggcagg ttgtccgggc
     7321 cgatatagcg cataaaggcc aatatcggcc aatgccacag ataaagcgaa taggaaatca
     7381 aaccggcggc aacggtgatt ttccattgga aaaatttttt aagcgggtgt tcgtaatgat
     7441 tgaaataaat cagcgcggca acagccagac agggaatcaa agcggcgggg cccgggaaat
     7501 aggcggtttg ttccgaatag gaaaacaggc aggttgacaa tatgcacacg gcaaacaatg
     7561 cgccggcggc ggcacagcgt ctgccgacgg caggttgccg gcagcgcatc cacacggcgg
     7621 tcagcgatcc gaccagcatt tcgcaggcgc gcaggtgggg caggtaatat ttatcgaacg
     7681 cggaaggcat aaaggaagcg gcaaggctta aggcgcacaa tgcggcgagg aagccgaact
     7741 gtacgcgcgg gcttttgcgg gcgacaagca gcagcaatat cggaaagaca aagtaaaatt
     7801 gttcttcgac cgacaaagac caaatgtgca gcaagggctt ttcttcctgc gcgggatcga
     7861 aataatcctt cccccttgca aaatacaggt tggaggcgaa acccaaggcg gtcagcgcgg
     7921 atttccataa aagaaagaaa tcatctttgg tgaataaaaa gaagccgcct gtcagcgttg
     7981 ccgccaatac ggcgaaaaat gcgggcagaa tccgcttgat gcggcggata taaaatgcct
     8041 tcagggaaaa cctccccccc cccgacattt cgcggtgaag aatcatcgtc atcaaaaagc
     8101 ctgaaatcac aaagaatata tcgacaccga gaaacccgcc cggcagccaa tttttttcga
     8161 tatggaacac gatgacggac aagacggcgg cggcgcgcaa tgtgtcgatg tccgggcggt
     8221 agggtaaggc ttggctcata atgtt
//
LOCUS       DAXHSF010000086         8079 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.086, whole genome shotgun sequence.
ACCESSION   DAXHSF010000086 DAXHSF010000000
VERSION     DAXHSF010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8079
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.086"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            167..1108
                     /gene="miaA"
                     /locus_tag="ACFM8S_001326"
     CDS             167..1108
                     /gene="miaA"
                     /locus_tag="ACFM8S_001326"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFC6314393.1"
                     /translation="MPTPKAFALLGPTAGGKTALALKIAETLPVEIISLDSALVYRDM
                     DIGTAKPSASERAFVPHHLIDIITPVQTYSAARFVEDCTRLIGEITARGKYPLIVGGT
                     MMYFRALTQGLNDLPEADACLRADLDEQKQMYGLDFLYRTLQQVDPETACRLKPNDSQ
                     RIGRALEVYYLTGKPMSEHLGRQSPHTLPFDLHTAALIPENRARLHENIALRFHLMLE
                     QGFIGEVENLRRRYPGLTADSPAIRCVGYRQAWEYLDGKTDFPAFVEKGIAATRQLAK
                     RQLTWLRKTPLDCVADPFSDGTSCTRLIEAAKRFFGA"
     gene            1112..1831
                     /gene="tadA"
                     /locus_tag="ACFM8S_001327"
     CDS             1112..1831
                     /gene="tadA"
                     /locus_tag="ACFM8S_001327"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFC6314394.1"
                     /translation="MLTTPPLAPKTVAALHRLGIRTLEELRQNGSVKAFLLLKASGLT
                     LTKSTLWQLESLLNGTLPQEITQHDKTRLFAELKNHPPVAAFPPQEEMEHFMREALRQ
                     AEQSAADGEIPVGAVIVSDGKIIASAHNTCIADCNVSRHAEINALAQAGSEMQNYRLD
                     GCDIYITLEPCAMCASALIQARIRRVIYGAAEPKTGAAGSIVNLFADKRLNTHTAIRG
                     GILQEECRAVLSRFFQNKRKG"
     gene            1836..2324
                     /locus_tag="ACFM8S_001328"
     CDS             1836..2324
                     /locus_tag="ACFM8S_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HFC6314395.1"
                     /translation="MTVLTVILALALIAVGTAGIVYPALPGLALMFAGTWLLAYVGGY
                     QIYGAGILWTVGLISLAGILADYVAGIWGTKYTGAGKLAVRGALVGSIIGIFFSLPGL
                     ILGPFIGAAAGELIDRRNMLQAGKAGLGTLLGLVVGTAFKIGCAVSILFILLVKYIAY
                     LF"
     gene            2390..3142
                     /gene="rlmB"
                     /locus_tag="ACFM8S_001329"
     CDS             2390..3142
                     /gene="rlmB"
                     /locus_tag="ACFM8S_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFC6314396.1"
                     /translation="MANQRLIYGFHAVNARLWQNPKSIVELYIQEGKSDARTREVLEK
                     AANENIRVYFADTDRLNAISKGARHQGVVGFIDASKNHVHLEDVLENLSEPPLLLILD
                     GITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVACGAAETVPYITVTNLAR
                     TLRELKEYGIWIIGTDMGGNADLYHCVLPDSAAWVMGNEGDGMRRLTREHCDMLVSIP
                     MFGTVESMNVSVSAGMVLSETRRQRVLKNEKA"
     gene            complement(3204..4595)
                     /locus_tag="ACFM8S_001330"
     CDS             complement(3204..4595)
                     /locus_tag="ACFM8S_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFC6314397.1"
                     /translation="MSGHMGKGADAPDLVYGLEDRPPFGNALLSAVTHLLAIFVPMIT
                     PALIVGGALELPVEMTAYLVSMAMVASGVGTYLQVNRFGPVGSGMLSIQSVNFSFVTV
                     MIALGAGMKEGGLTKDAMISTLLGVSFVGAFLVCFSAWLLPYLKKVITPTVSGVVVML
                     IGLSLVHVGITDFGGGFGAKADGTFGSMENLGLASLVLLIVLVFNCMKNPLLRMSGIA
                     VGLIAGYIVALFLGKVDFSALQNLPLVTLLVPFKYGFAFDWHAFIVAGAIFLLSVFEA
                     VGDLTATAMVSDQPIEGEEYTKRLRGGVLADGLVSVIATALGSLPLTTFAQNNGVIQM
                     TGVASRHVGKYIAVILVLLGLFPVVGRAFTTIPSPVLGGAMVLMFGLIAIAGVRILVS
                     HGIRRREAVIAATSVGLGLGVAFEPEVFKNLPVLFQNSISAGGITAVLLNLVLPEDKT
                     EAAVKFDTDHLEH"
     gene            complement(4610..4864)
                     /locus_tag="ACFM8S_001331"
     CDS             complement(4610..4864)
                     /locus_tag="ACFM8S_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314398.1"
                     /translation="MLPIVAYFTAGVKSGGSGMHGAAWRRMRRVCIFVFEIRLLRFAN
                     DIPAATVRNLFAGRARMPSEIEIEAVWRIRYNPRFGFFNI"
     gene            4902..5777
                     /gene="dapA"
                     /locus_tag="ACFM8S_001332"
     CDS             4902..5777
                     /gene="dapA"
                     /locus_tag="ACFM8S_001332"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFC6314399.1"
                     /translation="MLQGSLVALITPMNQDGSIHYEQLRDLIDWHIENGTDGIVAVGT
                     TGESATLSVEEHTAVIEAVVKHVAKRVPVIAGTGANNTVEAIALSQAAEKAGADYTLS
                     VVPYYNKPSQEGMYRHFKAVAEAAAIPMILYNVPGRTVVSMTNDTILRLSEIPNIVGV
                     KEASGNVGSNIELINRAPEGFVVLSGDDHTALPFMLCGGHGVITVAANAAPKLFADMC
                     RAALQGDIALARELNDRLIPIYDTMFCEPSPAAPKWAVSALGRCEPHVRLPLVPLTEG
                     GQAKVRAALKASGQL"
     gene            5786..6982
                     /gene="bamC"
                     /locus_tag="ACFM8S_001333"
     CDS             5786..6982
                     /gene="bamC"
                     /locus_tag="ACFM8S_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFC6314400.1"
                     /translation="MPSEAFRRHNATNTLISITQDDTMTHIKPVIAALALIGLAACSG
                     SKTEQPKLDYQSRSHRLIKLEVPPDLNNPDQGNLYRLPAGSGAVRASDLEKRRTPAVQ
                     QPADAEVLKSVKGVRLERDGSQRWLVVDGKSHAEIWPLLKAFWQENGFDIKSEEPAIG
                     QMETEWAENRAKIPQDSLRRLFDTVGLGGIYSTGERDKFIVRIEQGKNGVSDIFFAHK
                     AMKEVYGGKDKDTTVWQPSPSDPNLEAAFLTRFMQYLGVDGQQAENASAKKPTLPAAN
                     EMARIEGKSLIVLGDYGRNWRRTVLALDRIGLTVVGQNTERHAFLVQKAPNESNAVTE
                     QKPGLFKRLLGKGKAEKPAEQPELIVYAEPVANGSRIVLLNKDGSAYAGKDASALLGK
                     LHSELR"
     gene            complement(7039..7971)
                     /gene="pip"
                     /locus_tag="ACFM8S_001334"
     CDS             complement(7039..7971)
                     /gene="pip"
                     /locus_tag="ACFM8S_001334"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HFC6314401.1"
                     /translation="MYEIKQPFHSGYLQVSEIHQIYWEESGNPDGVPVIFLHGGPGAG
                     ASPECRGFFNPDVFRIVIIDQRGCGRSRPYACAEDNTTWDLVADIEKVREMLGIRKWL
                     VFGGSWGSTLSLAYAQTHPERVKGLVLRGIFLCRPSETAWLNEAGGVSRIYPEQWQKF
                     VAPIAENRRNRLIEAYHGLLFHQDEEVCLSAAKAWADWESYLIRFEPEEVDEDAYASL
                     AIARLENHYFVNGGWLQGDRAILNNIGKIRHIPTIIVQGRYDLCTPMQSAWALSKAFP
                     EAELRVVQAGHRAFDPPLVDALVQAVEDILPHLL"
BASE COUNT         2070 a         2637 c         1889 g         1483 t
ORIGIN      
        1 ttctctttga gctaaggcga ggcaacaccg tactggtttt tgttaatcca ctatataaag
       61 gcatacggta aacacgcccc cttgccgtcg gcgtgcttcc gcaaaccaaa atataacgtc
      121 ggcacgcttt ccgctataat ccgtattttt ccaaacctga aacgttatgc cgaccccgaa
      181 agcctttgcc ctgctcgggc cgaccgccgg aggcaaaacc gcgctcgccc tcaaaattgc
      241 cgaaaccctg cctgtcgaaa tcatcagcct cgattccgcg ctggtttacc gcgatatgga
      301 catcggcacg gcgaaaccgt ccgcctccga acgcgccttt gtcccgcacc accttatcga
      361 catcattacg cctgttcaga catacagtgc cgcccgtttt gtcgaagact gcacgcgtct
      421 gattggcgaa atcacggcgc gtgggaaata tccgctgatt gtcggcggca cgatgatgta
      481 tttccgcgcc ctgacccaag gtttgaacga tttgcccgaa gccgatgcct gcctgcgtgc
      541 cgacttggac gaacaaaaac aaatgtacgg cttggatttc ctctaccgca ccctgcaaca
      601 ggtcgatccc gaaacagcat gccgtctgaa accgaacgac agccagcgta tcggacgcgc
      661 tttggaagtt tattatctga ccggcaaacc gatgagcgaa cacctcggca gacaaagccc
      721 ccatacgctc cccttcgatt tgcataccgc cgccctgatt cccgaaaacc gcgcccgcct
      781 gcatgaaaac atcgccctgc gtttccacct gatgcttgaa cagggcttta tcggcgaagt
      841 agaaaacctg cgccgccgct atcccggcct gaccgccgac tcccccgcca tccgctgcgt
      901 cggctaccgt caggcatggg aatatctgga cggaaaaacc gatttcccgg catttgtcga
      961 gaaaggcatt gccgccaccc gccaacttgc caaacgccaa ctgacttggt tgcgcaaaac
     1021 acctttagac tgcgttgccg acccgttttc agacggcact tcatgcacac gcctgattga
     1081 ggcggcaaaa cggtttttcg gcgcataaac catgctgacc acaccgccgc tcgcccccaa
     1141 aaccgtcgcc gccctgcaca ggctcggtat ccgtacgctc gaagaactgc ggcaaaacgg
     1201 ttcggtcaaa gcctttctgc tgctcaaagc atcgggtttg accctcacga aaagcacgct
     1261 gtggcagctt gagtcgctgc tgaacggcac actcccgcaa gaaatcacgc aacacgacaa
     1321 aacgcgcctt tttgccgaat tgaaaaacca tccgcccgtc gccgcctttc cgccgcagga
     1381 agaaatggaa cacttcatgc gcgaagcact gcgccaagca gaacaatccg ccgctgacgg
     1441 cgaaataccc gtcggggcag tcatcgtttc agacggcaaa atcatcgcat cggcacacaa
     1501 cacctgcatt gccgactgca atgtcagccg gcacgccgaa atcaacgcct tggcacaggc
     1561 aggcagcgaa atgcaaaact accgccttga cggatgcgac atatatatca ccctcgaacc
     1621 ctgcgccatg tgcgcgtccg cactgataca ggcacgaatc aggcgtgtga tatatggtgc
     1681 ggcagaaccc aaaaccggag cggcaggcag cattgtcaac ctgtttgccg acaaacgcct
     1741 caatacgcac accgccatac ggggcggaat cctgcaagaa gaatgccgcg ccgtattaag
     1801 ccgttttttc caaaacaaaa gaaaaggttg aacctatgac cgtactgacc gtcatcctcg
     1861 ccctcgccct gatagccgtc ggtacggcgg gcatcgttta ccccgccctg cccggattgg
     1921 cattgatgtt tgctggaaca tggctgcttg cctatgtcgg cggctaccaa atttacggcg
     1981 caggcatcct gtggacggtc ggactcatca gccttgccgg catactggcg gactatgtgg
     2041 caggcatatg ggggacaaaa tataccggag cgggcaagct cgccgttcgc ggcgcattgg
     2101 tcggcagcat catcggcata tttttctccc ttcccggact aatactcggc ccctttatcg
     2161 gcgcggcggc aggcgaactc atcgacagac gcaatatgct tcaggcaggt aaagcgggct
     2221 tgggtacgct gttggggctt gtcgtcggta cggcgttcaa aatcggctgc gccgtatcca
     2281 tcttgtttat cctgttggtg aaatacatcg cctacctgtt ttaacccctt cagacggcat
     2341 acggataaat tgctataatg ccgtctgaaa tttccgacaa agcaccatca tggcaaacca
     2401 aagactcatc tacggcttcc acgccgtcaa tgcccgtttg tggcaaaacc ccaaatccat
     2461 cgtcgaactc tacatccaag aaggcaaatc agacgcacgc acgcgcgaag tgttggaaaa
     2521 ggcggcaaac gaaaacatcc gcgtatattt tgccgatacc gaccgcctca acgccatcag
     2581 caaaggcgcg cgccatcagg gcgtagtcgg atttatcgat gcctccaaaa accacgtcca
     2641 cctcgaagac gtattggaaa acttaagcga accgccgctg ctgctgatac tcgacggcat
     2701 caccgatccg cacaacctcg gcgcgtgcct gcgtaccgcc gacgcaatgg gcgtacatgc
     2761 cgtcatcgca ccgaaagaca aaagcgcggg gctgaacgcc accgtcagca aagtcgcctg
     2821 cggcgcggcg gaaaccgtcc cctatatcac cgtaaccaac ctcgcccgca ccctgcgcga
     2881 attgaaagaa tacggcattt ggatcatcgg caccgatatg ggcggcaacg ccgaccttta
     2941 ccattgcgtc ctgcccgaca gcgcggcatg ggtaatgggc aacgaaggcg acggtatgcg
     3001 ccgcctgacg cgcgaacact gcgatatgct ggtatccata ccgatgttcg gcacggtcga
     3061 aagcatgaac gtctccgtgt ctgccggaat ggtattaagc gaaacccgcc ggcaacgcgt
     3121 attaaaaaac gaaaaagcgt aagcctcaaa aacaatgccg tctgaaacag ggattctgtt
     3181 tcagacggca ttcgttttca aaatcagtgt tccaagtggt cggtatcaaa cttgaccgcc
     3241 gcctcggttt tatcttcggg caagaccaaa ttcagcaaga ctgccgtaat gccgccggcg
     3301 gaaatagagt tttggaacaa gacaggcagg tttttaaaca cttccggctc aaacgccacg
     3361 cccaagccca agccgaccga agtcgccgca atcaccgcct cgcgcctgcg gatgccgtgg
     3421 ctgaccaaaa tccgcacacc cgcaatcgca atcaagccga acatcaaaac catcgcgccg
     3481 cctaaaaccg gactcggaat cgtcgtaaac gcgcgtccga caacggggaa cagacccaac
     3541 agcaccaaaa tcacggcaat atatttgccc acatggcgcg aagccacgcc ggtcatctga
     3601 atcacgccgt tgttttgcgc aaacgttgtc agcggcagcg aacccaaagc cgtcgcaatc
     3661 accgacacca agccgtctgc caacacgccg ccgcgcaggc gtttggtgta ttcctcgcct
     3721 tcaatcggct gatcggacac cattgccgtt gcggtcaaat cgccgaccgc ctcaaacacg
     3781 ctcaacagga aaatcgcacc cgccacaata aatgcgtgcc agtcaaaagc aaaaccatat
     3841 ttaaacggta cgagcagcgt aaccagcggc aggttttgca gtgccgaaaa atccaccttg
     3901 cccaaaaaca gcgcgacgat atagccggca atcagaccga ccgcaatgcc gctcatccgc
     3961 agcagcgggt ttttcatgca gttgaacacc agcacaatca gcagcaccag cgatgccagc
     4021 cccaagtttt ccatcgagcc gaacgtgccg tccgcctttg cgccgaagcc gccgccgaaa
     4081 tcggtaatac cgacgtgtac caaactcaag ccgatcagca tcaccaccac accgctgacc
     4141 gtcggcgtaa tcactttttt caaatacggc agaagccacg ccgagaaaca caccaaaaac
     4201 gcgccgacaa acgatacgcc caagagcgtc gaaatcatcg catccttagt caaaccgccc
     4261 tctttcatcc ccgcgcccag cgcaatcatg acggtaacga acgagaaatt caccgactgg
     4321 atagacagca tccccgaacc gaccggcccg aagcggttga cctgcaaata agtgccgaca
     4381 cccgacgcaa ccatcgccat cgacacgaga tacgccgtca tctccaccgg cagttccagc
     4441 gcgccgccca caatcagcgc gggcgtaatc atcggcacga aaatcgccaa aagatgggta
     4501 accgcgctca acagcgcatt accgaacggc ggcctatcct ccaaaccgta caccaaatca
     4561 ggcgcatccg ccccttttcc catatgaccg ctcataatgt ttccttgctt caaatgttga
     4621 aaaaaccaaa gcgcggattg tagcgaatcc gccataccgc ttcaatttcg atttcggacg
     4681 gcatacgcgc ccgtccggca aacagattcc gaaccgtcgc cgccggaata tcgtttgcaa
     4741 aacgcaataa cctgatttca aacacaaaaa tacaaacccg acgcatccgc cgccaagccg
     4801 ccccgtgcat accggacccg ccggatttca cacctgcggt aaagtaggct acaatcggaa
     4861 gcattaccgc cccctacacc acaactgata aaaggttctg tatgttacaa ggtagcttgg
     4921 ttgccctgat taccccgatg aatcaagacg gcagcatcca ttacgaacaa ctccgcgact
     4981 taatcgactg gcacattgaa aacggcacgg acggcatcgt cgccgtcggc acgacaggcg
     5041 aatccgccac cctctccgtc gaagaacaca ccgccgttat cgaagccgtc gtcaaacacg
     5101 ttgccaaacg cgtccccgtc atcgccggca caggggcgaa caacaccgtc gaagccatcg
     5161 ccctttccca agccgccgaa aaagccggcg cggactacac cctctccgtc gtcccctatt
     5221 acaacaaacc ttcgcaagaa ggcatgtacc gccatttcaa agccgttgcc gaagccgccg
     5281 cgattccgat gatactctac aacgttcccg gccgtaccgt cgtcagcatg actaacgaca
     5341 ccatcctgcg cctttcagaa atccccaata tcgtgggcgt taaagaagcc agcggcaacg
     5401 tcggcagcaa catcgaactc atcaaccgcg cccccgaagg cttcgtcgtc ctgtccggcg
     5461 acgaccacac cgccctaccg ttcatgctct gcggcggaca cggcgtgatt accgtagccg
     5521 ccaacgccgc gcccaagctc tttgccgata tgtgccgcgc cgcgcttcaa ggcgatatcg
     5581 cccttgcacg cgaactcaac gaccggctga ttccgattta cgacaccatg ttctgcgaac
     5641 ccagccccgc cgcaccgaaa tgggcggtat ccgccctggg cagatgcgaa ccgcacgtcc
     5701 gcctgccgct cgttccgctg acggaaggcg gacaggcaaa agtccgcgcc gccctgaaag
     5761 cctcaggaca actttaaata tagccatgcc gtctgaagcg ttcagacggc ataacgcaac
     5821 aaacacttta atatccatca cacaggatga cacgatgacc catatcaaac ccgtcattgc
     5881 cgcgctcgca ctcatcgggc ttgccgcctg ctccggcagc aaaaccgaac agcccaagct
     5941 cgactaccaa agccggtcgc accgcctgat caaactcgaa gtcccacctg atttgaacaa
     6001 ccccgaccaa ggcaacctct accgcctgcc tgccggttcg ggcgccgtcc gcgccagcga
     6061 tttggaaaaa cgccgcacac ccgccgtcca acagcctgcc gatgccgaag tattgaaaag
     6121 cgtcaaaggt gtccgcctcg agcgcgacgg cagccaacgc tggctcgttg tcgacggcaa
     6181 gtctcatgcc gaaatctggc cgctcctgaa agccttttgg caggaaaacg gcttcgacat
     6241 caaatccgaa gaacccgcca tcggacaaat ggaaaccgag tgggcggaaa accgtgccaa
     6301 aatcccccaa gacagcttgc gccgcctatt cgacacagtc ggtttgggcg gcatctactc
     6361 caccggcgag cgcgacaaat tcatcgtccg tatcgaacag ggcaaaaacg gcgtttccga
     6421 catcttcttc gcccacaaag ccatgaaaga agtgtacggc ggcaaagaca aagacacgac
     6481 cgtatggcag ccctccccgt ccgatcccaa cctcgaagcc gctttcctga cgcgctttat
     6541 gcaatatttg ggcgttgacg gacagcaggc ggaaaacgca tcggcaaaaa aacctaccct
     6601 tcccgccgcc aacgaaatgg cgcgtatcga aggcaaaagc ctgattgtct taggcgacta
     6661 cggcagaaac tggcggcgca ccgtgctcgc cctcgaccgc atcgggctga ccgtcgtcgg
     6721 tcaaaacacc gaacgccacg ctttcctggt tcaaaaagcc ccgaacgaaa gcaatgcagt
     6781 taccgaacaa aaacccggcc tgttcaaacg cctgctgggc aaaggcaaag cggagaaacc
     6841 tgccgaacag ccggaactga ttgtctatgc cgagcctgtc gccaacggct cgcgcatcgt
     6901 cctgctcaac aaagacggca gcgcatatgc cggcaaagac gcatccgcat tattgggcaa
     6961 actccattcc gaactgcgtt aaaccattca aaacaaaagc agcttccaaa cagaagctgc
     7021 ttttttatgc ggaacttttt acaacaaatg gggcaaaata tcctcaactg cctgaaccaa
     7081 cgcatccacc aaaggcggat cgaacgcacg atgccctgcc tgaaccaccc tcaattccgc
     7141 ttcgggaaag gctttcgaca gcgcccacgc actctgcatc ggcgtacaca aatcataccg
     7201 cccctgcacg ataatggtcg ggatatgccg tattttgccg atattgttca aaatcgccct
     7261 atcgccctgc aaccagccgc cgttgacaaa ataatggttt tccaaacgcg cgattgccag
     7321 tgaggcataa gcatcttcat ccacttcctc cggctcgaaa cggatcagat agctttccca
     7381 atccgcccaa gcctttgcgg cagacaggca cacttcttca tcttgatgaa acagcaatcc
     7441 gtgatacgcc tcaatcagcc ggttccgccg attttcagca atcggcgcga caaatttttg
     7501 ccattgttcc ggataaatcc ggctcacacc gcccgcctcg ttcagccacg ccgtttcaga
     7561 cggcctgcac aaaaatatcc cgcgcaatac caatcccttt acccgttcag gatgggtttg
     7621 ggcataagcc agcgacaaag tgctgcccca cgaaccgccg aacaccagcc atttcctgat
     7681 acccagcatt tcacggactt tttcaatatc cgccaccaaa tcccaagtcg tattgtcttc
     7741 cgcacaagca tacgggcgcg aacgtccgca accgcgctgg tcgatgatga cgatgcggaa
     7801 cacatcggga ttgaaaaaac cccgacattc aggcgaagcc cccgcgcccg gcccgccatg
     7861 taaaaaaatg accggcacac cgtcgggatt gcccgactct tcccaataaa tttgatgaat
     7921 ttcagacacc tgcaagtatc cgctatgaaa aggctgtttt atctcataca tcatcttctc
     7981 ccatattgct tctgacaatc cgaacattcc cacacgcaac atcacgcacg agacacaagc
     8041 cgccccgaaa ggcaaatgcc gaaaaatcgc gatatagtg
//
LOCUS       DAXHSF010000087         8032 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.087, whole genome shotgun sequence.
ACCESSION   DAXHSF010000087 DAXHSF010000000
VERSION     DAXHSF010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8032
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.087"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(67..291)
                     /locus_tag="ACFM8S_001335"
     CDS             complement(67..291)
                     /locus_tag="ACFM8S_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HFC6314402.1"
                     /translation="MDAVQELERRIVELEIQSALQEDVIAGLNAMVAELRQTLDLQQA
                     QLRLLYQKMQDRNPDAQEPYSLRDEIPPHY"
     gene            complement(292..1680)
                     /locus_tag="ACFM8S_001336"
     CDS             complement(292..1680)
                     /locus_tag="ACFM8S_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HFC6314403.1"
                     /translation="MNAVVVAVIVMLVLSLSRVHVVLSLTVGAFVGGAVAGMPLQNIA
                     DAAGQVSQAGIIPVFNKGLEGGAKIALSYAMLGAFAMAITHSGLPQQLAGAVVRKLNR
                     GGMPDSVRSGEGAVKWLLLSIILVMGIMSQNIIPIHIAFIPMIVPPLLLVFNRLKIDR
                     RLIACVITFGLVTTYMFLPYGFGAIFLNEILLGNIHSAAPQLDVKNINVMAAMAIPAL
                     GMLAGLLLAFVHYRKPRLYQSNNADTAGNADAANRPQPSAYRSLAAAVAIAVCFAIQL
                     MYEDSLVLGAMLGFAVFMMLGVINRDKANDVFGEGIKMMAMVGFIMIAAQGFAAVMNA
                     TGHIQPLVESSMAIFGNSKGMAALAMLVVGLLVTMGIGSSFSTLPIIAAIYVPLCVGL
                     GFSPLATVAIVGTAGALGDAGSPASDSTLGPTMGLNADGQHDHIRDSVIPTFIHYNIP
                     LLIAGWIAAMVL"
     gene            complement(1766..2587)
                     /gene="prmC"
                     /locus_tag="ACFM8S_001337"
     CDS             complement(1766..2587)
                     /gene="prmC"
                     /locus_tag="ACFM8S_001337"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFC6314404.1"
                     /translation="MTFDKWLGLSKLPKNEARMLLQYVSEYTRVQLLTRGGEEMPDEV
                     RQRADRLAQRRLNGEPVAYILGVREFYGRRFTVNPSVLIPRPETEHLVEAVLARLPEN
                     GRVWDLGTGSGAVAVTVALERPDAFVRASDISPPALETARKNAADLGARVEFAHGSWF
                     DTDMPSEGKWDIIVSNPPYIENGDKHLSQGDLRFEPQIALTDFSDGLSCIRTLAQGAP
                     DRLAEGGFLLLEHGFDQGAAVRGVLAENGFSGVETLPDLAGLDRVTLGKYMKHLK"
     gene            complement(3102..4586)
                     /gene="tldD"
                     /locus_tag="ACFM8S_001338"
     CDS             complement(3102..4586)
                     /gene="tldD"
                     /locus_tag="ACFM8S_001338"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFC6314405.1"
                     /translation="MPYNQAPDFLCADTMHPTYSAVQARLLEANRLSPELLAKSLCII
                     GAHHVDYADIYCQRTAYESWHLEEGIVKSGSFQINQGVGVRAVSGDKTAFAYADSLCI
                     DSINRSARAVRAIGAAGGKVSAKMPSETRGKPVCSASDPIAGLDSAAKVALLNKVEAI
                     AKAADPRIVQVMAGLTCEYDMVYLARLDGKHAADIRPMVRLNVTVIAKQGERREQGSA
                     GGGGRYDLAYFDENLVHRFVDAAVKQALTNLESRPAPAGEMTVVLGNGWPGVLLHEAV
                     GHGLEGDFNRKGTSVFSGRIGERVAAKGVTVVDQGDIAGRRGSLNIDDEGNETRRTVL
                     IEDGILVGYMQDETNARLTGTQSTGNGRRESYASAPMPRMTNTFMENGSYEPEEIIAS
                     IDKGIYAVNFGGGQVDITSGKFVFSASEAWWVEGGRLQYPVKGATIIGNGPEVLKHVS
                     MIGNDTALDSGVGVCGKEGQSVPVGVGQPTLRIDAGLTVGGSAI"
     regulatory      4661..4764
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4830..6053
                     /gene="cytX"
                     /locus_tag="ACFM8S_001339"
     CDS             4830..6053
                     /gene="cytX"
                     /locus_tag="ACFM8S_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HFC6314406.1"
                     /translation="MSGNASSPSSSSAIGLIWFGAAVSIAEISTGTLLAPLGWQRGLA
                     ALLLGHAVGGALFFAAAYIGALTGRSSMESVRLSFGKRGSVLFSVANMLQLAGWTAVM
                     IYAGATVSSALGKVLWDGESFVWWALANGALIVLWLVFGARKTGGLKTVSMLLMLLAV
                     LWLSAEVFSTAGSTAAQVSDGMSFGTAVELSAVMPLSWLPLAADYTRHARRPFAATLT
                     ATLAYTLTGCWMYALGLAAALFTGETDVAKILLGAGLGAAGILAVVLSTVTTTFLDAY
                     SAGASANNISARFAETPVAVGVTLIGTVLAVMLPVTEYENFLLLIGSVFAPMAAVLIA
                     DFFVLKRREEIEGFDFAGLVLWLAGFILYRFLLSSGWESSIGLTAPVMSAVAIATVSV
                     RLFFKKTQSLQRNPS"
     gene            6050..7150
                     /locus_tag="ACFM8S_001340"
     CDS             6050..7150
                     /locus_tag="ACFM8S_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFC6314407.1"
                     /translation="MTRIAILGGGLSGRLTALQLAEQGYQIALFDKGCRRGEHAAAYV
                     AAAMLAPAAEAVEATPEVVRLGRQSIPLWRGIRCRLNTHTMMQENGSLIVWHGQDKPL
                     SSEFVRHLKRGGVADDEIVRWHADDIAEREPQLGGRFSDGIYLPTEGQLDGRQILSAL
                     ADALDELNVPCHWEHECAPEDLQAQYDWLIDCRGYGAKTAWNASAEHGSTLRGIRGEV
                     ARVYTPEITLNRPVRLLHPRYPLYIAPKENHVFVIGATQIESESQAPASVRSGLELLS
                     ALYAVHPAFGEADILEIATGLRPTLNHHNPEIRYNRARRLIEINGLFRHGFMISPAVT
                     AAAVRLAVALFDGKDAPERDEESGLAYIRRQD"
     gene            7171..7788
                     /gene="thiE"
                     /locus_tag="ACFM8S_001341"
     CDS             7171..7788
                     /gene="thiE"
                     /locus_tag="ACFM8S_001341"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFC6314408.1"
                     /translation="MTFPPLKSPLKFYAVVPTADWVGRMVKAGADTVQLRCKALHGDE
                     LKREIARCVAACQGSHTQLFINDHWREAIEAGAYGVHLGQEDMDTADLAAIAAAGLRL
                     GLSTHSVAELDRALSVHPSYVASGAIFPTTTKQMPTAPQGLEKLREYVKQAGSTPVVA
                     IGGIDLNNARAVLATGVSSLAAVRAVTEAANPEAVVKAFQALWDG"
BASE COUNT         1729 a         2465 c         2179 g         1659 t
ORIGIN      
        1 ttaggtttgc atcgtcggaa tattcaaaca cagccttttt taaggaaatc cggatacggc
       61 ggcgcatcaa taatgcggcg gaatctcgtc gcgcagggaa tacggctctt gcgcgtcggg
      121 attcctgtcc tgcatttttt gatacagcag cctcaactga gcctgctgca aatccagcgt
      181 ctgccgcaat tccgccacca tcgcgttcag gccggcgatt acgtcctcct gaagcgcgga
      241 ttggatttcc agttcgacaa tacggcgttc caactcttga accgcgtcca tttacagcac
      301 catcgcggca atccagccgg caatcagcag cgggatgttg tagtggatga aggtcgggat
      361 aacggaatcg cggatgtggt cgtgctgccc gtcggcgttc agccccatcg tcgggcccag
      421 cgtggaatcg gacgcaggcg aaccggcatc gcccaacgcc cccgccgtgc cgacaatggc
      481 gacggtggca agcggcgaaa aacccaaacc gacacacaaa ggcacataaa tcgcggcaat
      541 aatcggcaaa gtggaaaagg acgaaccgat gcccatcgtt accaaaagcc ccaccaccag
      601 catcgccaat gccgccatac ctttgctgtt gccgaatatc gccatactgc tttccaccag
      661 cggctgaata tgcccggtcg cattcatcac ggcggcaaaa ccctgcgcgg caatcataat
      721 gaagccgacc atcgccatca tcttgatacc ttcgccgaat acgtcgtttg ccttgtcgcg
      781 gttaatgacc cccaacatca taaatacggc gaaaccgagc atcgcgccca acaccagcga
      841 gtcttcatac atcaactgga tggcaaagca tacggcaatg gcgacggcgg cggccaggct
      901 gcggtaggcg gacggctgcg gacggtttgc cgcatcggcg ttgcccgccg tatcggcatt
      961 gttgctttgg tacaggcgcg gtttgcggta atggacaaac gccagcagga gtccggccag
     1021 cattcccaac gcgggaatcg ccattgccgc catcacgtta atgtttttca catcaagctg
     1081 cggcgcggcg gaatggatgt tgcccaacag gatttcgttc aaaaaaatcg cgccgaagcc
     1141 gtaaggcagg aacatataag tcgtaaccag cccgaaagtg atgacgcacg caatcaggcg
     1201 gcggtcgatt ttcaggcggt tgaacaccaa aagcagcggc gggacaatca tcgggataaa
     1261 ggcgatgtgg atggggatga tgttctgact cataatgccc atcacaagga tgatggaaag
     1321 cagcagccat ttgaccgcgc cctcgcccga acgcacgctg tcgggcatac cgccccggtt
     1381 cagcttgcgg acgaccgcgc cggcaagctg ctgcggcagg ccggaatggg taatcgccat
     1441 tgcaaacgcg ccgagcatcg cataagaaag cgcaatcttc gcaccgcctt ccaaaccttt
     1501 gttgaacacg gggataatcc ccgcctgact gacctgtccc gccgcatcgg caatgttttg
     1561 cagcggcata cccgccaccg cgccgccgac aaacgcgccg accgtcaggc tcaataccac
     1621 gtgcacgcgc gacagcgaca gcaccagcat aacgattacg gcaactacga ctgcattcat
     1681 tgtttatcgc tccaaaccta taaatgttta catatcgaaa cacatcataa cccaataacg
     1741 ggaaacccgc caattttgca aacaattatt tcaaatgctt catatacttc cccagcgtaa
     1801 ccctgtccaa acccgccaaa tccggcaggg tttccactcc tgaaaaacca ttctccgcca
     1861 acacgccgcg caccgccgcg ccctgatcga aaccgtgttc cagcaataaa aaaccgcctt
     1921 ccgccaaacg gtcgggcgcg ccttgcgcca aggtacggat gcagcttagg ccgtctgaaa
     1981 agtcggtcag cgcgatttgc ggctcaaacc gcaaatcgcc ttgcgacaaa tgtttatcgc
     2041 cgttttcgat atagggcggg ttggacacga tgatgtccca tttcccttca gacggcatat
     2101 cggtgtcgaa ccacgaaccg tgtgcaaatt cgacccgcgc gcccaaatcc gccgcatttt
     2161 tccgcgccgt ttcaagggcg ggcgggctga tgtcggatgc gcgcacaaac gcatcggggc
     2221 gttcgagcgc gacggttacg gcaaccgcgc cgctgcccgt ccccaaatcc cacacgcgcc
     2281 cgttttcggg caggcgcgcc aatacggctt cgaccaaatg ttcggtttcg gggcgcggaa
     2341 tcagcacgct cggattgact gtaaagcgtc tgccataaaa ttcgcgcaca cctaaaatat
     2401 aggcaaccgg ctcgccgttc agacggcgtt gcgccagcct gtccgcccgc tgtcggactt
     2461 cgtccggcat ttcttccccg ccccgcgtca acaactgcac gcgcgtatat tccgaaacat
     2521 attgcagcag cattcttgct tcatttttag gcagttttga caagcccaac catttatcaa
     2581 acgtcatttt tatcccgtct gccgctgatg cggcttttct ttccttattc tttccggcaa
     2641 acgtaccgat ggtggcaacc gcaaatgcgg cataccacaa ataaaatcct gcaccgtagc
     2701 gcacaatatc cgatgtattc cctgcttcat cgacgtatac ggctttcaca ctgaaagcca
     2761 ccaacgccaa gccccaaagt gccgcatgga caggcacgac cttcttccgc aacgccagca
     2821 aaacaatggc cgccaaccaa acataattcg catagaccgc acaatacctg atatccaaag
     2881 aagcaaatat cgaccccaaa atcaaaacgg tcaaaccctc catgcttcca tggttgccca
     2941 aataaaatgc aacattggat aaagacgcta tccacagggc aaccgacacc agcaacatca
     3001 ctatgggaaa acttggtttc cgattctgtt cctgcatggt tttatcctaa tgtaaaaggc
     3061 cgcctgaaaa cctttcagac ggcatcgtgc cggattccgc gtcagattgc gctgccgccg
     3121 acggtcagcc cggcatcgat ccgcaaagtc ggctgtccca cgccgacggg gacgctctgc
     3181 ccttctttgc cgcacacgcc gacaccgctg tcgagcgcag tatcgttgcc tatcatggaa
     3241 acgtgtttca gcacttcggg gccgttgccg ataatggtcg cgcctttgac ggggtattgc
     3301 agcctgccgc cttccaccca ccatgcttcg gacgcgctga acacaaattt gccgctggta
     3361 atgtccacct gtccgccgcc aaagttgacg gcgtaaatgc ctttgtcgat ggacgcgatg
     3421 atttcttccg gctcatagct gccgttttcc ataaaggtat tggtcatgcg cggcataggg
     3481 gcggaagcgt aactttcgcg gcggccgttg ccggtggatt gcgtacccgt caggcgggca
     3541 ttggtttcgt cctgcatata gccgactaaa atgccgtctt caatcaatac ggtgcggcgg
     3601 gtttcgttgc cttcgtcgtc gatgttgagc gaaccgcgcc ggccggcaat atcgccctga
     3661 tccacaacgg taacgccttt ggcggcgacg cgctcgccta ttctgccgga aaagacgctg
     3721 gttcccttgc ggttgaaatc gccttccaaa ccgtgtccga ccgcttcgtg cagcaacacg
     3781 cccggccagc cgttgcccaa aacgacggtc atttcgccgg cgggcgcggg gcgggattcg
     3841 aggttggtga gtgcctgttt gacggcggca tcgacaaacc gatgaaccaa gttttcatcg
     3901 aaataagcca agtcgtagcg tccgccgccg cccgcgctgc cctgttcgcg gcgttcgccc
     3961 tgtttggcga taacggtaac gttcaggcgc accatcgggc ggatgtcggc ggcgtgtttg
     4021 ccgtccagac gggcgaggta aaccatatcg tattcgcagg tcaaaccggc catcacttgc
     4081 acgatgcgcg gatcggcggc tttggcgatt gcttccactt tgttcaacag cgcgactttg
     4141 gcggcggaat cgaggccggc aatggggtcg gacgcggaac aaaccggctt gccgcgcgtt
     4201 tcagacggca ttttggcgga caccttgccg cctgccgccc caatcgcgcg gacggcgcga
     4261 gcggaacggt ttatcgaatc gatgcacagg ctgtcggcgt aggcaaaggc ggttttgtcg
     4321 cccgaaacgg cgcgcacgcc cacgccctga ttgatttgga agctgcccga tttgacgatg
     4381 ccctcttcca aatgccagct ttcataagcg gtgcgctggc agtagatgtc ggcgtaatcg
     4441 acgtggtgcg cgccgatgat gcacaggctt ttggcgagca gttcggggga aaggcggttg
     4501 gcttcgagca gccgcgcctg tacggcggaa taggtcggat gcatagtgtc ggcgcataaa
     4561 aaatcagggg cttgattata cggcatttgt tatggaaatg cgctgtgccg ccgaaaatgt
     4621 aagaattttg ccaacgcacg ttgctttgtg taaacttaaa gctttcttgt cggagtgccg
     4681 ccgccgggcg gctgagattg cgaaagcaga atccgtagaa cctgtcgggg taatgcctgc
     4741 gtaggaaaca aaacctcccc gccctttcgg gcttccgttc ccttttccgc acttccccgc
     4801 cccattttca tgttttttaa ggacttgata tgtcgggcaa tgcctcctct ccttcatctt
     4861 cctccgccat cgggctgatt tggttcggcg cggcggtatc gattgccgaa atcagcacgg
     4921 gtacgctgct tgcgcctttg ggctggcagc gcggtctggc ggctctactt ttgggtcatg
     4981 ccgtcggcgg cgcgctgttt tttgcggcgg cgtatatcgg cgcactgacc ggacgcagct
     5041 cgatggaaag cgtgcgcctg tcgttcggca aacgcggttc agtgctgttt tccgtggcga
     5101 atatgctgca actggccggc tggacggcgg tgatgattta cgccggcgca acggtcagct
     5161 ccgctttggg caaagtgttg tgggacggcg aatcttttgt ctggtgggca ttggcaaacg
     5221 gcgcgctgat tgtgctgtgg ctggttttcg gcgcacgcaa aacaggcggg ctgaaaaccg
     5281 tttcgatgct gctgatgctg ttggcggttc tgtggctgag tgccgaagtc ttttccacgg
     5341 caggcagcac cgccgcacag gtttcagacg gcatgagttt cggaacggca gtcgagctgt
     5401 ccgccgtgat gccgctttcc tggctgccgc ttgccgccga ctacacgcgc cacgcgcgcc
     5461 gcccgtttgc ggcaaccctg acggcaacgc tcgcctacac gctgaccggc tgctggatgt
     5521 atgccttggg tttggcagcg gcgttgttca ccggagaaac cgacgtggca aaaatcctgc
     5581 tgggcgcagg tttgggtgcg gcaggcattt tggcggtcgt cctctccacc gttaccacaa
     5641 cgtttctcga tgcctattcc gccggcgcga gtgcgaacaa catttccgcg cgttttgcgg
     5701 aaacacccgt cgctgtcggc gttaccctga tcggcacggt acttgccgtc atgctgcccg
     5761 ttaccgaata tgaaaacttc ctgctgctta tcggctcggt atttgcgccg atggcggcgg
     5821 ttttgattgc cgactttttc gtcttgaaac ggcgtgagga gattgaaggc tttgactttg
     5881 ccggactggt tctgtggctt gcgggcttca tcctctaccg cttcctgctc tcgtccggct
     5941 gggaaagcag catcggtctg accgcccccg taatgtctgc cgttgccatt gccaccgtat
     6001 cggtacgcct tttctttaaa aaaacccaat ctttacaaag gaacccgtca tgacccgtat
     6061 cgccatcctc ggcggcggcc tctcgggaag gctgaccgcg ttgcagcttg cagaacaagg
     6121 ttatcagatt gcacttttcg ataaaggctg ccgccggggc gaacacgccg ccgcctatgt
     6181 tgccgccgcc atgctcgcgc ctgcggcgga agcggtcgaa gccacgcccg aagtggtcag
     6241 gctgggcagg cagagcatcc cgctttggcg cggcatccga tgccgtctga acacgcacac
     6301 gatgatgcag gaaaacggca gcctgattgt gtggcacggg caggacaagc cattatccag
     6361 cgagttcgtc cgccatctca aacgcggcgg cgtagcggat gacgaaatcg tccgttggca
     6421 cgccgacgac atcgccgaac gcgaaccgca actcggcgga cgtttttcag acggcatcta
     6481 cctgccgacc gaaggccagc tcgacgggcg gcaaatattg tctgcacttg ccgacgcttt
     6541 ggacgaactg aacgtccctt gccattggga acacgaatgt gcccccgaag acctgcaagc
     6601 ccaatacgac tggctgatcg actgccgcgg ctacggcgca aaaaccgcgt ggaatgcttc
     6661 tgccgaacac ggcagcaccc tgcgcggcat acgcggcgaa gtggcgcggg tttacacacc
     6721 cgaaatcacg ctcaaccgcc ccgtgcgcct gctgcacccg cgttatccgc tctacatcgc
     6781 cccgaaagaa aaccacgtct tcgtcatcgg cgcgacccaa atcgaaagcg aaagccaagc
     6841 acctgccagc gtgcgttccg ggctggaact cttatccgca ctctatgccg tccaccccgc
     6901 cttcggcgaa gccgacatcc tcgaaatcgc caccggcctg cgccccacgc tcaaccacca
     6961 caaccccgaa atccgttaca accgcgcccg acgcctgatt gaaatcaacg gccttttccg
     7021 ccacggtttc atgatctccc ccgccgtaac cgccgccgcc gtcagattgg cagtggcact
     7081 gtttgacgga aaagacgcgc ccgaacgtga tgaagaaagc ggtttggcgt atatccgaag
     7141 acaagattaa agccgtctga aaggaacccc atgaccttcc cgccactcaa atccccgctc
     7201 aaattctacg ccgtcgtccc caccgccgat tgggtggggc gcatggtcaa agcaggtgcc
     7261 gacacggtgc aactgcgctg caaggccctg cacggcgatg aattgaaacg cgaaatcgcc
     7321 cgctgtgtcg ccgcctgtca gggcagccat acgcagcttt tcatcaatga ccactggcgc
     7381 gaggcaatcg aagcaggcgc gtacggcgtg catctcggac aagaagacat ggacaccgcc
     7441 gaccttgccg ccatcgccgc cgccggtttg cgcttgggtt tgagtacgca ctccgttgcc
     7501 gaactcgacc gcgccctgtc cgtacaccct agctacgtcg ccagcggcgc gattttcccg
     7561 accacgacca aacaaatgcc caccgcccct caaggcttgg aaaaactgcg tgaatatgtg
     7621 aaacaggcag gcagcacgcc cgtcgtcgcc attggcggca tcgatttgaa caacgcccga
     7681 gccgtactcg ccaccggcgt ttcctcactc gccgccgtcc gcgccgtaac cgaagcggca
     7741 aatcccgaag cggtggttaa agcgtttcag gctttgtggg atggataaaa ccgaaagaag
     7801 aaaattcaat tgccgtgtag gcaaaactta gcccgttatc gcaaacatac ttaactttaa
     7861 atgtggcata tcatcaaatt ccgtcattcc cgcgcaagcg ggaatccgcc ttaaaacttg
     7921 agaaaccatc atttgaaaaa cagtttccga atttcaaaaa tggattcccg cctgcgtggg
     7981 aatgacggca accggccggt tgcgtatcaa aaaataaagt aattcggcta ga
//
LOCUS       DAXHSF010000088         8020 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.088, whole genome shotgun sequence.
ACCESSION   DAXHSF010000088 DAXHSF010000000
VERSION     DAXHSF010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8020
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.088"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(68..814)
                     /locus_tag="ACFM8S_001342"
     CDS             complement(68..814)
                     /locus_tag="ACFM8S_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFC6314409.1"
                     /translation="MDFRFDIIYEYRWMFLYGALTTLGLTVVATAGGSVLGLLLALAR
                     LIHLEKAGAPMRVLAWALRKVSLLYVTLFRGTPLFVQIVIWAYVWFPFFVHPSDGILV
                     SGEAAIALRRGYGPLIAGSLALIANSGAYICEIFRAGIQSIDKGQMEAARSLGLTYPQ
                     AMRYVILPQALRRMLPPLASEFITLLKDSSLLSVIAVAELAYVQNTITGRYSVYEEPL
                     YTVALIYLLMTTFLGWIFLRLEKRYNPQHR"
     gene            complement(798..977)
                     /locus_tag="ACFM8S_001343"
     CDS             complement(798..977)
                     /locus_tag="ACFM8S_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314410.1"
                     /translation="MNGKCRLKTARPAFLRRRATAVCRDKKVQAAVFTVKWRIILQRR
                     QFRRFLIRTVYGFSF"
     gene            complement(993..1664)
                     /locus_tag="ACFM8S_001344"
     CDS             complement(993..1664)
                     /locus_tag="ACFM8S_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFC6314411.1"
                     /translation="MQIKKIMKWLPVALSLLGALGYTGYGSEAVRTAVAVLDVLGAAG
                     DAGSDAPARRRASAKSGHRYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQA
                     YGTRSRDNLRAAAEGRKVSVRVFDTDRYQREVAQVSVGKTDLNLMQVQDGAAWHYKSY
                     AKEQQDKADFADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWMDAVGE
                     WLGIW"
     gene            complement(1733..2404)
                     /gene="rpiA"
                     /locus_tag="ACFM8S_001345"
     CDS             complement(1733..2404)
                     /gene="rpiA"
                     /locus_tag="ACFM8S_001345"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFC6314412.1"
                     /translation="MTTQDELKRIAAEKAVEFVPENEYIGIGTGSTINFFIEALGKSG
                     KKIKGAVSTSKKSSELLAQYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHL
                     NEKIVASASEKFVCIADESKYVSRLGKFPLPVEVVESARSLVSRKLLAMGGQPELRIG
                     YTTFYGNQIVDVHGLNIDQPLTMEDEINKITGVLENGIFARDAADVLILGTEEGAKVI
                     YPCQG"
     gene            complement(2481..2963)
                     /gene="ispF"
                     /locus_tag="ACFM8S_001346"
     CDS             complement(2481..2963)
                     /gene="ispF"
                     /locus_tag="ACFM8S_001346"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFC6314413.1"
                     /translation="MTNIRIGQGYDVHQLTEGRKLILGGVEIPFEKGLLGHSDADALL
                     HAVTDALLGAAGLGDIGSHFPDTAAEFKDADSRVLLRAAYQSVQAQGWQAVNVDTTVI
                     AQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
                     "
     gene            complement(2995..3684)
                     /gene="ispD"
                     /locus_tag="ACFM8S_001347"
     CDS             complement(2995..3684)
                     /gene="ispD"
                     /locus_tag="ACFM8S_001347"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFC6314414.1"
                     /translation="MKRKNIALIPAAGIGARFGADKPKQYVEIGSKTVLEHTLGIFER
                     HEAVDLTVVVVSPEDTFADKVQTAFPQVRVWKNGGQTRAETVRNGVAKLLEIGLASAD
                     DNILVHDAARCCLPSEALTRLIEQAGNAAEGGILAISIADTLKRAEGGQISATVERTS
                     LWQAQTPQLFRAGLLHRALAAENLDGITDEASAVEKLGIRPLLVQGDARNLKLTQPQD
                     AYIVRLLLNAV"
     gene            complement(3681..4415)
                     /gene="dnaQ"
                     /locus_tag="ACFM8S_001348"
     CDS             complement(3681..4415)
                     /gene="dnaQ"
                     /locus_tag="ACFM8S_001348"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFC6314415.1"
                     /translation="MTTRQIILDTETTGLYADGGDRLVEFAGLEMVNRQMTDKNLHLY
                     VHPERDMPEEAARVHGLTIEVLEGKNAPPFAEVGRQIADFLRGAELIIHNAKFDVGFL
                     NMEFRRMGLPTVEELGCTVTDTLAMAREMFPGQKASLDALCNRFSVDRSKRVLHGALI
                     DCELLGKVYLAMTRRQFDLMGEAEEEEPAAKPVASAEMKLGAKLKVIKADEAELAAHG
                     QYLDGLGEACIWRKEAVPSENGGTDA"
     gene            complement(4571..5881)
                     /locus_tag="ACFM8S_001349"
     CDS             complement(4571..5881)
                     /locus_tag="ACFM8S_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC6314416.1"
                     /translation="MYAKKGGLGLVKSRRFAPLFATQFLGAFNDNVFKTALFVMIGFY
                     GLGQNGFLPAGQMLNLGALLFILPYFLFSSLSGQLGNKFDKAVLARWVKVLEMIIMAV
                     AAYGFYIRSAPLLLACLFCMGAQSTLFGPLKYAILPDYLDDKELMMGNSLIESGTFVA
                     ILFGQILGTAVAGVPPYIVGILVLLVAVGGTVGSLFMPSVPAKAADTQIEWNIVRGTK
                     SLLSETVRHKPVFTAIIGISWFWFVGAVYTTQLPTFTQIHLGGNDNVFNLMLALFSIG
                     IAAGSVLCAKFSRERLMLAWVTVGALGLTVCGLVLVWLTHGHRFEGLNGIFWFLSQGW
                     AYPVMAVMTLIGFFGGFFSVPLYTWLQTASSETFRAHAVAANNIINGIFMVSAAVLSA
                     VLLFLFDSISLLYLIVALGNIPLAVFLIKRERRFLGAVAIRKKP"
     gene            complement(5871..6056)
                     /gene="ccoS"
                     /locus_tag="ACFM8S_001350"
     CDS             complement(5871..6056)
                     /gene="ccoS"
                     /locus_tag="ACFM8S_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HFC6314417.1"
                     /translation="MESMFILVPISIILAFVIGWFFWWSGKNGQFDDLEGPAHRILMD
                     DDSTSKLIEKERENDVR"
     gene            complement(6056..6355)
                     /locus_tag="ACFM8S_001351"
     CDS             complement(6056..6355)
                     /locus_tag="ACFM8S_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314418.1"
                     /translation="MHYFSIHDQSGRHIGFFILLADDESEARPQSGRFAVKLEGGMEN
                     HVLSPFGQTEIPQYWRVVKDRIELFFDEAPVGTLRNEYLTVSGQTFVLNDLTGNM"
     gene            6706..7902
                     /locus_tag="ACFM8S_001352"
     CDS             6706..7902
                     /locus_tag="ACFM8S_001352"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFC6314419.1"
                     /translation="MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYI
                     TFKVNGEKHKVDLSAHPDHTGAVEALMEELKAHGLDSRIGAIGHRVVSGGELYSESIL
                     VDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKDLPNVVVFDTSFHQTMPEVAYKY
                     AVPQELYEKYGLRRYGAHGTSYRFVADETARFLGKDKKDLRMVIAHLGNGASITAVAN
                     GESRDTSMGLTPLEGLVMGTRSGDIDPSVFGFLAENANMTIAQITEMLNKKSGLLGIS
                     GLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGGLDALVFTGGIGENS
                     DIIRERVIGYLGFLGLNIDQEANLKARFGNAGVITTADSKAVAVVIPTNEELMIAHDT
                     ARLSGL"
BASE COUNT         1895 a         2404 c         1956 g         1765 t
ORIGIN      
        1 ataaatttac cacaaaaaag ctgcctcaaa tgaatacccg ggcagctttt tgttgatatg
       61 actccaatca gcggtgttgc ggattgtaac gtttttccaa acgcaggaat atccagccta
      121 agaaagtcgt catcaacaga taaatcaggg cgacggtgta aagcggttct tcataaaccg
      181 aataccggcc cgtaatcgta ttctgaacat acgccaactc cgccacagca atgaccgaca
      241 gcagcgagct gtctttcaag agcgtgatga actcgctcgc caaaggcggc agcatacggc
      301 gcaatgcctg cggcagaatc acatagcgca tcgcctgcgg ataggtcagc cccaaagaac
      361 gcgccgcctc catctgtcct ttgtctatag actggatgcc cgcgcggaaa atctcacaga
      421 tatacgcccc cgagttggcg atcagtgcca aagaaccggc aatcagcggc ccgtatccgc
      481 gacgcagcgc gattgccgcc tcgccgctaa ccaaaatgcc gtctgaagga tggacgaaaa
      541 acggaaacca cacatacgcc caaatcacaa tctgcacaaa cagcggcgta ccccggaaca
      601 gcgtaacata cagcagcgaa accttacgca acgcccacgc cagcacgcgc atcggcgcac
      661 cggctttttc caagtgaatc aggcgcgcca acgccaacaa cagacccaat accgaaccgc
      721 ccgccgtcgc cacgaccgtc agccccaagg tcgtcagtgc gccgtaaaga aacatccagc
      781 ggtattcgta aataatgtca aaacgaaaat ccataaaccg tccgtatcaa aaaccggcgg
      841 aactgccgcc gttgcaaaat aatccgccat tttaccgtaa aaaccgccgc ctgaactttt
      901 ttatcgcggc agacggcggt tgcgcgtctc cgcaaaaatg cagggcgcgc ggttttcaga
      961 cggcatttgc cgttcaaagc cgtgcggtgt ctttaccaaa tgcccaacca ttcgcccacg
     1021 gcatccatcc aatccttatt gcccccgccg ctcctgcctg cccggcggta cgcccacggc
     1081 gcttgcggat ttttagcttt ccacaatcct ttgcgttccc tttccgcctg aatttgagcg
     1141 tcggcataat cggcaaaatc cgccttatcc tgctgttctt tagcataact tttataatgc
     1201 cacgccgccc cgtcctgcac ctgcatcagg ttcaaatcgg ttttgccgac agaaacctgc
     1261 gccacttcgc gctggtagcg gtcggtgtcg aacacgcgga cgctgacttt cctgccttcc
     1321 gccgccgcgc gcaggttgtc gcgcgaacgc gtgccgtaag cctgtttcat ctccggcgcg
     1381 tcgatatacg ccatccggat tttgtgtttc gcgccgtcgc cgtcgataac gtgaagggtg
     1441 tcgccgtcat agactttgga caccgtgcct gtgtagcggt ggccggattt cgccgatgct
     1501 cggcggcggg cgggcgcgtc ggaacccgcg tcccctgccg cgccgagtac gtcgagtacg
     1561 gcaaccgccg tccgcaccgc ctcgctgccg taccccgtat aacccaacgc acccaaaagc
     1621 gacaaggcga cgggaagcca tttcatgatt tttttaatct gcatattttt caaatgccga
     1681 tgccgtctga acatatcgga atcggatttc agacggcatc ttaacgtcag gattaccctt
     1741 ggcagggata gatgactttc gcaccctctt ccgtccccaa aatcaacaca tcggcggcat
     1801 cgcgggcgaa tatgccgttt tcgagcacgc cggtgatttt gttgatttcg tcttccatcg
     1861 tcagcggctg atcgatattc aagccgtgga catcgacgat ttggttgccg taaaacgtgg
     1921 tgtagccgat acgcagttcg ggctgtccgc ccatagcgag cagtttgcgc gaaacaagag
     1981 agcgcgcgct ttcgacgact tccacaggca gagggaattt gcccaaacgt gaaacatatt
     2041 tgctttcatc cgcaatgcag acgaattttt cggatgcgct ggcgacgatt ttttcgttga
     2101 ggtgtgcgcc gccgccgcct ttaatcattt gcagggcgtg gttcacttca tccgcaccgt
     2161 caatatagac cgccaacccc gatacttcgt tcagggaaac gacgggaata tcatattggg
     2221 caagcagttc actggatttt ttggaagtag ataccgcgcc tttgattttt ttgccgctct
     2281 tacccaaggc ttcgatgaaa aagttgatgg tcgaaccggt accgatgccg atgtattcgt
     2341 tttcgggtac gaattcgact gctttttcgg cggcgatgcg cttgagttcg tcttgtgtcg
     2401 tcatattttt gtcctttggg aaaccgtatc aacaaacagc cgccatctta acattttttt
     2461 gcacgtcctg cccgccgcgt tcaaatcctt accagcaata ccgccgcctg cgcctctatg
     2521 ccttccatgc gcccgagata accgagtttt tcgttggttt tgcctttgat gttgacgcac
     2581 gaaatgtcta tgcccaaatc ggcggcgatg ttggcacgca tttgcggaat atgcggcgcg
     2641 agtttgggtt tctgtgcaat cacggtcgta tcgacattga ccgcctgcca accctgcgcc
     2701 tgaacgcttt gatacgccgc acgcaaaagg acgcggctgt ccgcatcttt gaactctgcg
     2761 gcggtgtcgg ggaaatggct gccgatatcg cccaaacctg ccgcaccgag cagcgcgtcg
     2821 gtaacagcgt gcagcagcgc atcggcatcg gagtgtccga gcagcccttt ttcaaatggg
     2881 atttcaactc cgccaagtat cagctttctg ccttcggtca gttggtggac atcgtagccc
     2941 tgtccgatac ggatgttcgt catcgtttgt gttcctgatg ttttgaattg aagttcagac
     3001 ggcattgagc agcagcctga cgatgtatgc gtcctgcggc tgcgtcagtt tcaaattgcg
     3061 cgcgtcgccc tgcaccagca aagggcggat gcccaatttt tccacggcgg acgcttcatc
     3121 ggtaatgccg tccaagtttt ccgcagccaa tgcgcggtgc agcagccccg cgcggaaaag
     3181 ctgcggcgtt tgtgcctgcc aaaggctcgt ccgctcgaca gtggcactga tttgtccgcc
     3241 ttctgcgcgt ttgagcgtat cggcaatgga aattgccaaa atcccgcctt cggcggcgtt
     3301 gcccgcctgt tctatcaacc gcgtcaaagc ttcagacggc aggcagcagc gcgcggcatc
     3361 gtgtaccaga atattgtcat ccgccgatgc caagccgatt tccaacagtt ttgccacacc
     3421 gtttcggacg gtttcggcgc gggtctgtcc gccgtttttc cacacccgaa cctgtggaaa
     3481 tgccgtctga accttatcgg caaacgtgtc ttcgggcgag acgacaacga cggtcaaatc
     3541 gacggcctca tgccgttcaa aaatcccaag cgtatgttct aaaacggttt tgcttccgat
     3601 ttcgacatat tgcttgggtt tgtccgcacc gaaacgcgcc ccgatgccgg cggcgggaat
     3661 cagcgcgata tttttgcgct tcatgcgtcc gtcccgccgt tttcagacgg cacggcttcc
     3721 ttgcgccaga tacaggcttc gcccaagccg tccaaatatt gcccgtgcgc cgccagttcg
     3781 gcttcatccg ccttaatcac tttcagtttg gcaccgagtt tcatttcggc agatgcaaca
     3841 ggtttggctg ccggctcttc ctcctcggcc tcccccatca ggtcgaactg ccgccgcgtc
     3901 atagcaagat agactttgcc caaaagttcg cagtcgatca atgcgccgtg caggacgcgc
     3961 ttgctgcggt cgacggaaaa acggttgcac agggcatcta ggctggcttt ctgcccgggg
     4021 aacatttcgc gcgccatcgc cagggtatcg gtaacggtac agccgagttc ctcaacggtc
     4081 ggcaacccca tccggcggaa ctccatattg aggaagccca cgtcgaattt ggcattgtgg
     4141 ataatcagtt ccgcaccgcg caggaaatcg gcaatctgcc tgccgacctc tgcaaacggc
     4201 ggcgcgtttt tcccttccaa aacctctatc gtcagaccgt gcactctcgc cgcctcttcg
     4261 ggcatatcgc gctcggggtg gacatagagg tgcaggtttt tgtcggtcat ttggcggttg
     4321 accatttcca gaccggcaaa ctcgaccaag cggtcgccgc cgtcggcata cagaccggtg
     4381 gtttcggtat cgaggatgat ttggcgtgtc gtcatatcgg tgtctttctt ctatcttcgt
     4441 aaattgctta ttttttaagc aatgtatttt tctgttttca tttcaatgca caaacccact
     4501 tattcacagt gtgttcacaa cattgggcag gcggattgtg tattttgggg acaatttttt
     4561 cagacggcat tcaaggtttt ttcctgattg ccaccgcgcc taaaaaccgc ctttcgcgct
     4621 taatcaaaaa taccgccaac ggaatattgc ccaaggcgac aatcagatac agcagtgaaa
     4681 tgctgtcaaa caaaaacagc agcaccgcac tcaaaacggc ggcggaaacc ataaagatac
     4741 cgttaatgat attgttagca gcaacagcgt gggcacggaa agtctcgctg ctggcggttt
     4801 gcagccaggt atagagcgga acggagaaaa atccgccgaa aaagccgatc agcgtcatca
     4861 ccgccatcac ggggtatgcc catccttgcg ataaaaacca aaaaatgccg ttcagccctt
     4921 caaaacggtg tccgtgcgtc agccacacca aaaccaggcc gcaaaccgtc aaacccaacg
     4981 caccaaccgt tacccaagcc aacatcagcc gttccctgct gaacttggca cacagtaccg
     5041 aaccggcggc aataccgatg gaaaacaggg caagcatcag gttgaaaaca ttgtcgttgc
     5101 cgcccagatg gatttgggta aaggtcggca gttgcgtggt ataaaccgcg ccgacaaacc
     5161 aaaaccaaga aataccgata atggcggtaa aaacgggctt gtgccgcacc gtttcactca
     5221 gcagggattt tgtgccacgg acaatattcc actcaatttg tgtatcggca gccttggcgg
     5281 gtacagacgg cataaacagg ctgccgaccg tgcctccgac ggcgaccagc aaaaccagta
     5341 tcccgacaat ataaggcggt acacctgcca ccgccgttcc caaaatctga ccgaacagga
     5401 tggcgacaaa cgtacccgat tcaatcaggc tgttgcccat catcaactct ttgtcgtcga
     5461 gataatcggg caggatggcg tatttcagcg gcccgaacag cgtcgattgc gcgcccatgc
     5521 aaaacagaca cgccaaaagc agcggggcag accggatata aaacccgtat gccgccaccg
     5581 ccataatgat catttccagc accttgaccc aacgcgccaa aacggccttg tcgaatttgt
     5641 tacccaactg ccccgacagc gaggaaaaca ggaaatacgg caaaataaac agcaacgcgc
     5701 ccaagttcaa catctgtccg gcaggcagga agccgttttg ccccaaaccg taaaacccaa
     5761 tcatcacaaa cagcgcggtt ttgaacacat tgtcgttgaa cgcgccgaga aactgcgtag
     5821 cgaaaagagg tgcgaaacgg cggcttttaa ccagtcccaa accgcctttt ttagcgtaca
     5881 tcgttttccc tctctttttc aatcagttta cttgtcgaat catcatccat caggatgcgg
     5941 tgcgccggcc cttccaagtc gtcaaactgc ccgtttttgc ccgaccacca aaaaaaccag
     6001 ccgatgacaa acgccaaaat aatgctgatg ggcaccaata taaacatgct ttccatcaca
     6061 tattccctgt caaatcgttc aaaacaaaag tctgccccga cacggtcaga tattcgttac
     6121 gcaaagttcc gacgggggct tcgtcaaaaa acagctcgat acggtctttg accacgcgcc
     6181 aatattgggg gatttccgtc tgaccgaacg gcgacaggac atgattttcc attccgcctt
     6241 caagtttgac ggcaaaacgc ccgctttgcg gccgtgcttc cgattcgtcg tcggcaagca
     6301 ggatgaaaaa gcctatatgc cgtcccgatt ggtcatgaat actgaaataa tgcataaatt
     6361 tcccacccgc cttttttcag acgacaccaa ctaaaaacag ggcgaatgta ccagtttgga
     6421 cgggaagaat gcaaagaaat tctccctccc ccagccgaaa acaccggcaa accgcatatc
     6481 cccctttttt ccgtcaaaat gcccgacttc cgccattttc acgcaaacgc ccgattaagc
     6541 caagcaattg caaagatttt ttgctagaat agcctgcttc ttttatcaac cttttcagac
     6601 ggccccacta ctttcccgcc caggaaggca aaacggattc ggcacgaatc cggttagtat
     6661 ccgtgtccga ttccaatgcc gtctgaaact ttccggagta agaaaatgtc ccaaaaattg
     6721 atcttggttt tgaactgcgg cagctcgtcc ctcaaaggcg cggtcctgga taacggcagc
     6781 ggcgaagtcc tgctcagctg ccttgccgaa aaactcaacc tgcccgatgc ctacatcaca
     6841 ttcaaagtaa acggcgaaaa acacaaagtc gatctgtccg cacatcccga ccacaccggc
     6901 gcggtcgaag ccctgatgga agaactcaaa gcccacggcc tcgacagccg catcggcgcc
     6961 atcggccacc gcgtcgtcag cggcggcgaa ctgtacagcg aatccatcct cgttgacgac
     7021 gaagtcattg ccggcatcga aaaatgcatc ccgctcgccc ccctgcacaa ccccgcccac
     7081 ctcttgggcc tgcgtgccgc gcaaagcatt ttcaaagacc tgcccaacgt cgtcgtattc
     7141 gatacctcct tccaccaaac catgcccgaa gtcgcctaca aatacgccgt tccgcaggag
     7201 ttgtatgaaa aatacggcct gcgccgttac ggcgcgcacg gtaccagcta ccgcttcgtc
     7261 gccgacgaaa ccgcgcgctt cctcggcaaa gacaaaaaag acctgcgtat ggtcattgcc
     7321 cacttgggca acggcgcgtc catcaccgcc gtcgccaacg gcgaatcgcg cgacaccagt
     7381 atgggcctga ccccgctgga agggctggta atgggtacgc gcagcggcga catcgatcct
     7441 tccgtattcg gcttcctcgc cgaaaacgcc aatatgacca tcgcccaaat cactgaaatg
     7501 ctgaacaaaa aatccggtct gctcggcatt tccggcctgt ccaacgactg ccgcaccatt
     7561 gaagaagaag ccgccaaggg gcataaaggc gcgaaattgg ccttggatat gtttatctac
     7621 cgccttgcca aatacatcgg cagtatggcg gttgccgcag gcggtttgga cgcactggtc
     7681 tttaccggcg gcatcggcga aaactccgac atcatccgcg aacgcgtgat cggctacttg
     7741 ggcttcctcg gtctgaacat cgaccaagaa gccaacctga aagcccgctt cggcaacgcc
     7801 ggcgtgatta ccactgccga cagcaaagcc gttgccgtgg tcattccgac caacgaagag
     7861 ctgatgattg cccacgacac tgcccgtttg agcggcttgt aaggttttat ccgcacacga
     7921 actgcctctg gaaatggagg cagttttttt atccggcttt ccatgcttaa acagcactgc
     7981 ctcttttcag acattgacgg ttgcagccga ctacctgaac
//
LOCUS       DAXHSF010000089         7993 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.089, whole genome shotgun sequence.
ACCESSION   DAXHSF010000089 DAXHSF010000000
VERSION     DAXHSF010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7993
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.089"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(65..1312)
                     /gene="fabF"
                     /locus_tag="ACFM8S_001353"
     CDS             complement(65..1312)
                     /gene="fabF"
                     /locus_tag="ACFM8S_001353"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFC6314420.1"
                     /translation="MSQRRVVITGLGQVSPVGNTVAEAWDTLLAGKSGIGAITRFDAS
                     DINSRVAGEVRGFDIGQYISAKEARRMDVFIHYGIAAALQAIADSGLDDVENLDKDRI
                     GVNIGSGIGGLPSIEVTGKAVIEGGARKINPFFIPGSLINLISGHVTILKGYRGPSYG
                     MVSACTTGAHAIGNSARLIKYGDADIMVAGGAEGAISTLGVGGFAAMKALSTRNDDPA
                     TASRPWDKGRDGFVIGEGAGILVLEELEHAKKRGAKIYAEIVGFGMSSDAYHITAPNE
                     EGPALAVTRALKDAGINPEDVDYVNAHGTSTPLGDANETKALKRAFGEHAYKTVVSST
                     KSMTGHLLGAAGGVEAVYSILAIHDGKIPPTINIFEQDVEAGCDLDYCANEARDAEID
                     VAISNSFGFGGTNGTLVFKRFKG"
     gene            complement(1468..1704)
                     /gene="acpP"
                     /locus_tag="ACFM8S_001354"
     CDS             complement(1468..1704)
                     /gene="acpP"
                     /locus_tag="ACFM8S_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFC6314421.1"
                     /translation="MSNIEQQVKKIVAEQLGVNEADVKNESSFQDDLGADSLDTVELV
                     MALEEAFGCEIPDEDAEKITTVQLAIDYINAHNG"
     gene            1936..2943
                     /locus_tag="ACFM8S_001355"
     CDS             1936..2943
                     /locus_tag="ACFM8S_001355"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFC6314422.1"
                     /translation="MYPLARRILFALDAEKAHHFTLDALYTVYKLGLIPVTGNRTKPV
                     KLMGMDLPNPVGLAAGLDKNGEYIDALGALGFGFIEIGTVTPKPQPGNPQPRLFRVPE
                     HQGIINRMGFNNHGIDTMIRNIEKSKFSGVLGINIGKNAVTPIENAADDYLICLEKAY
                     AHASYITVNISSPNTKNLRALQGGDELSALLEALKNKQAQLASVHGKYVPLAVKIAPD
                     LDEAQIEDIAHVVKSVEMDGIIATNTTIDKSSLGSHPLAGEQGGLSGRPVHEKSNRVL
                     KLLADHIDGKLPIIGVGGIMEGRDAADKIRLGATAVQVYSGLIYKGPALVKECLKALA
                     R"
     gene            2959..3138
                     /locus_tag="ACFM8S_001356"
     CDS             2959..3138
                     /locus_tag="ACFM8S_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314423.1"
                     /translation="MPSERTFCRSDGIFISFFRLTPLENPLRAALFETKQTPAANRTI
                     LIRSQSKSGGKLLNI"
     gene            3276..3599
                     /locus_tag="ACFM8S_001357"
     CDS             3276..3599
                     /locus_tag="ACFM8S_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314424.1"
                     /translation="MFKDELNEFIRLISDPESELDEWYLSDFKDEHIWEMQSYEAFSC
                     LREAVPYLFAYPRYGYELLEIISALKETSDTTELFYEPGIVPLLIDLYKEDSYLVNMV
                     KRIFK"
     gene            3686..3967
                     /locus_tag="ACFM8S_001358"
     CDS             3686..3967
                     /locus_tag="ACFM8S_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314425.1"
                     /translation="MENGQRNNRFSLEKRIFYLEHSGRYLMICALSDYSQNKHTVVMA
                     NFIYPDEKTDWRNLDDLFNELVLEELQASFMDWHPTVEEAISRHLEDFS"
     gene            4020..4493
                     /locus_tag="ACFM8S_001359"
     CDS             4020..4493
                     /locus_tag="ACFM8S_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HFC6314426.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYGNKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLINKSNILELKPVLEDLASEMRDYSPKNWLYVLLSDVFHRKEEFEDPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSAHTYEENIARLYSHWEHYLNNGGGQG"
     gene            4507..4812
                     /locus_tag="ACFM8S_001360"
     CDS             4507..4812
                     /locus_tag="ACFM8S_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314427.1"
                     /translation="MPSEALSGLQTAFCFPTVYKLSLFLDRNNDLIYLKRLEDMIMGF
                     WNGVAKAVGEGMIEAGNEHKALKMEYAEKSSEELHEIVKSDGFLKIPHGRKVRLMLF"
     gene            5030..7720
                     /gene="glnE"
                     /locus_tag="ACFM8S_001361"
     CDS             5030..7720
                     /gene="glnE"
                     /locus_tag="ACFM8S_001361"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFC6314428.1"
                     /translation="MSDNRLDTARRHSLFLARQLDNGKLKPEIFLPMLDKVWTEADFQ
                     AFADWDKIRAEENEEELARQLRGLRRYVVSQIIVRDINRISDLNEVTRTITLFADFAV
                     NTALDFAYAYYRDMYGTPIGRYTKSPQHLSVVAMGKAGGYELNVSSDIDLIFIYPESG
                     DTDGRRERGNQEFFTKVGQKLIALLNDITADGQVFRVDMRLRPDGDSGALVLSETALE
                     QYLITQGREWERYAWCKGRVVTPYPNDIKALVRPFVFRKYLDYGAYEAMRKLHRQIRS
                     EVSKKGMADNIKLGAGGIREVEFIAQIFQMIRGGQMRALQLKGTQETLKKLAETGIMP
                     SENVETLLAAYRFLRDVEHRLQYWDDQQTQTLPTSPEQRQLLAESMGFDSYSAFSDGL
                     NIHRNKVNQLFNEILSEPEEQTQDNSEWQWAWQEKPDEEERLGRLKEYGFDAETIATR
                     LDQIRNGHKYRHLSAHAQPRFDAIVPLFVQAAAEQNNPTDTLMRLLDFLENISRRSAY
                     LAFLNEHPQTLAQLAQIMGQSSWVAAYLNKYPILLDELISAQLLDTAFDWQALAAALS
                     DDLKACGGDTEAQMDTLRRFQHAQVFRLAVQDLAGLWTVESLSDQLSALADTILAAAL
                     LCAWADMPKKHRDTPQFAIVGYGKLGGKELGYASDLDLVYLYDDPHPDAGDVYSRLAR
                     RLTNWLSTATGAGSLYETDLRLRPNGDAGFLAHSIAAFEKYQRENAWTWEHQSLTRAR
                     FICGTPEIQTAFDRIRTEMLTAERDQTALAGEIIEMREKMFPTHPPADSNVKYARGGV
                     VDVEFIVQYLILAHARQYPQLLDNYGNIALLNIAADCSLIDKNLAEQSRTAYRFYRRQ
                     QHNTKLRDAAKTEVTGELLAHYGNVRKLWRDVFGEEAATA"
BASE COUNT         1968 a         2050 c         2119 g         1856 t
ORIGIN      
        1 ctataaaaat gccgtctgaa acggtttcag acggcatttc gatgtcggcg gcggctttgc
       61 ggaatcagcc tttgaagcgt ttgaagacca gcgtgccgtt ggtgccgccg aagccgaagg
      121 agttggaaat ggcaacgtcg atttccgcgt cgcgcgcttc gttggcgcag tagtccaaat
      181 cgcagccggc ttcaacgtct tgttcaaaaa tgttgatggt cggcgggatt ttgccgtcgt
      241 gtatcgccaa aatgctgtac acggcctcca cgccgcccgc cgcgccgagc aggtggccgg
      301 tcatggattt ggtcgagctg acgacggttt tgtaggcgtg ttcgccgaac gcgcgtttga
      361 gggctttggt ttcgttggca tcgcccaagg gggtggacgt gccgtgcgcg ttgacgtaat
      421 ccacgtcttc gggattgatg ccggcatctt tcagcgcgcg ggtaacggca agggcggggc
      481 cttcttcgtt cggcgcggtg atatggtaag catcggaact catgccgaag ccgacgattt
      541 cggcgtagat tttcgcgccg cgttttttgg cgtgttccaa ttcttccaac accaatatgc
      601 ccgcgccttc gccgataacg aagccgtcgc ggcctttgtc ccacggacgg gaagcggtgg
      661 cggggtcgtc gttgcgggtg gagagggctt tcatcgcggc aaaaccgccc acgcccaaag
      721 tgctgattgc gccttccgcg ccgccggcaa ccattatgtc cgcgtcgccg tatttgatca
      781 gtcgggcgga attgccgatg gcgtgcgcgc cggtggtaca ggcggaaacc atcccgtagc
      841 tcgggccgcg gtagcctttg agaatggtaa cgtgtccgga aatcaggttg atcagcgaac
      901 cggggataaa gaaagggttg attttgcgcg cgccgccttc gattacggct ttgccggtga
      961 cctcgatgct gggcagtccg ccgatgccgg aaccgatgtt cacgccgatg cggtctttgt
     1021 cgaggttttc cacatcgtcc aagcccgaat cggcgattgc ctgcaatgcg gcggcaatgc
     1081 cgtagtggat gaatacgtcc atccggcgcg cttctttcgc gctgatgtat tgtccgatgt
     1141 cgaaaccgcg cacttcgccg gcgacacggc tgttgatgtc ggatgcgtca aagcgggtaa
     1201 tcgcgccgat gccgcttttg ccggcgagca gggtgtccca agcctctgcg acagtgttgc
     1261 cgacagggga aacctgacct aagcctgtaa tgactactct tctctgactc atgataacct
     1321 cgctgttggt tgtcggaatg ggggcatatg cggctgtcgt gcagatgccg tctgtaattt
     1381 tcggcagggg ttcaaacagt ttgccatata agggaaaagc ctctattgcg cggtgcagca
     1441 gaggctgttg tgtcgggcga cgaccggtta gccgttgtgg gcattgatgt agtcgatagc
     1501 cagttggacg gtggtgattt tttcggcatc ttcgtcgggg atttcgcagc cgaatgcttc
     1561 ttccaaagcc ataaccagct ccacggtgtc caaagaatcc gcgcccaagt cgtcttggaa
     1621 agaagattcg tttttcacgt cggcttcgtt tacgcccagt tgttcagcaa caattttttt
     1681 aacttgttgt tcgatgtttg acatatcagt cgttccttta tgccttgcgg caggttgttt
     1741 aagggaaata aatcggtggt attgtaccga cttttaatag agttttctat ctaatgacta
     1801 ttatatcaat atttgccgat ttgtacattt ttgggtgcgg cgggttttgt cgttcaagtt
     1861 tgacctgtgt gccgtatgtt tggcgggatt tcggttaaaa tggcggcatt tccatctgaa
     1921 gcagaaagcc ctgtcatgta tccacttgcc cgccgcatcc tgtttgcact cgatgccgaa
     1981 aaagcccacc acttcacgct cgacgcgctc tacacggttt ataaattggg tctgattccc
     2041 gtaaccggca accgtaccaa acctgtaaaa ttgatgggta tggatttgcc caaccctgtc
     2101 ggacttgccg ctggactcga caaaaacggc gaatacatcg acgcattggg cgcgctcggc
     2161 tttggtttca tcgaaatcgg cacggtaacg cccaaaccgc agcccggcaa cccgcagccg
     2221 cgcctcttcc gcgttcccga acaccaaggc atcatcaacc gcatgggttt caacaaccac
     2281 ggtatcgaca ccatgatacg caacatcgaa aaaagtaaat tcagtggcgt attgggcatc
     2341 aacatcggta aaaacgcggt tacacccatc gaaaacgctg ccgatgatta tttaatctgc
     2401 cttgaaaaag cctacgcgca cgcaagctac attaccgtca acatttcctc gcccaacacc
     2461 aaaaacctcc gcgcgctgca aggtggcgac gagttgagcg cattgcttga ggctttgaaa
     2521 aacaaacagg cacagcttgc ctctgtacac gggaaatacg ttccgctcgc cgtcaaaatc
     2581 gcccccgatt tggatgaagc acaaatcgaa gacatcgccc acgttgtcaa atccgtcgaa
     2641 atggacggca tcatcgctac caataccacc atcgacaaat caagtctcgg cagccatccg
     2701 ctcgcaggcg agcagggcgg tttgagcggg cggcccgttc atgaaaaaag taatcgggtg
     2761 ttgaagctgt tggcagacca catagacggc aagctgccga ttatcggcgt aggcggcatt
     2821 atggaaggca gggacgcggc agataaaatc cgcttgggcg caactgccgt ccaggtgtac
     2881 agcggattga tatacaaagg tccggcattg gtcaaagaat gtttgaaggc tttggcgcga
     2941 tgacgcgatc cgcccaaaat gccgtctgaa cgcacgtttt gccgttcaga cggcattttc
     3001 atttcctttt tccgcctgac gccccttgaa aatcccttac gcgccgccct gtttgaaaca
     3061 aagcaaacgc ccgccgcgaa ccggacaatc ttgatacgca gccaaagcaa gtccggcggt
     3121 aagttgttaa atatataagc aattgataca aaaaataatt ttatactgtg gcggacggct
     3181 tgggaaaacg caaaatatga acggcgttga aattcatgat gtggaaaata aggatacggc
     3241 ggataatcaa aagattttat atttaaggat ttgatatgtt taaggacgaa ctaaatgaat
     3301 ttatccgatt gatatccgat ccggaaagtg aattggatga atggtatttg agtgatttta
     3361 aagatgaaca tatatgggaa atgcaaagtt acgaagcatt ttcatgtttg cgggaagcag
     3421 tcccctattt gtttgcttat ccccgatacg ggtatgaact gttggagata atttccgcgt
     3481 taaaagaaac atctgatacg acagaattat tttatgagcc gggtatcgtg cctttgctga
     3541 ttgatttata taaagaagat agttatctgg taaatatggt caaacggata ttcaaatgat
     3601 gaaaggggcg cgggcgtttg cttggcggaa ggcaggtaac tcaatttgaa ggacgcggaa
     3661 gccgatacgt taaaaaggat gaaaaatgga aaacgggcaa cggaataacc ggttttcttt
     3721 ggaaaaacga attttttatc tggagcattc ggggcggtat ctgatgattt gcgccttatc
     3781 ggattattcc caaaacaaac atactgtcgt tatggcaaat ttcatctatc cggatgaaaa
     3841 aacggattgg cggaatttgg atgatttatt caatgagttg gttttagagg aattgcaggc
     3901 ttcatttatg gattggcatc cgactgttga agaggcaatc agccgtcatt tggaagactt
     3961 ttcgtaacag gcgtcaatcg gaaggggcgg aatgacgggg caggtaaccg gagtaaaaaa
     4021 tgaaccttga tttaaccgcg caaaaagtcc gtctttcttg gaaggatatt ctgtgggggt
     4081 atgggaataa atacttgggt tgggctgatg tggcagctta tgcccgaaaa atgacgcttt
     4141 cagatcatga tgaacgtgtg ttcaaactat ctttaatcaa caaatccaat attcttgaat
     4201 taaagcctgt tctggaagat ttggcttcgg aaatgaggga ttattcccct aaaaattggc
     4261 tgtacgtcct cttaagcgat gtattccata gaaaagaaga atttgaggat cctttggggg
     4321 aagttgaaaa aatttatgca gattttgatt atccggaaga aatagaatca tttgtcaggt
     4381 atatgccgcc caaagacggt tatattcctt ctgcccacac ctatgaagaa aatattgccc
     4441 ggttatattc tcactgggaa cactatttga acaacggcgg agggcagggt taaaaccggc
     4501 aatccgatgc cgtctgaagc attatccggc cttcagacgg cattttgttt tccgacagtt
     4561 tataaactgt cgttgtttct tgacagaaac aacgacctta tttatttgaa acgattggag
     4621 gacatgatta tgggtttttg gaatggtgtg gcaaaagcag tgggagaggg aatgattgaa
     4681 gccggcaatg agcataaggc gttgaaaatg gaatatgcgg agaaatcaag tgaggagctg
     4741 catgaaatcg tcaagagtga tggtttttta aaaattccac acgggagaaa agtgcggctt
     4801 atgctgtttt aaaagagcgt ggcgaggtgt gaacaggaaa cggcggcatt tgccgctgtt
     4861 ttttatcggc aggcatccgt ctggatgtcg gggcaaggtt tcagacggca tcgaaggttg
     4921 ctatgatata gtggcttgac tttaaaccgg tacggcatcc cctcgccctg tcctgatttg
     4981 aagtgaatcc gccatcatct tcctgccatt ttttcaaacg gaatccgaaa tgtccgacaa
     5041 ccgcctcgac accgcccgcc gccattccct cttcctcgcc cgccagctcg acaacggcaa
     5101 actcaagccc gaaattttcc tgcctatgct ggacaaggtt tggaccgaag cggatttcca
     5161 agcctttgcc gactgggaca aaatccgtgc ggaagaaaac gaggaagaat tggcgcggca
     5221 gttgcgcggg ttgcgccgtt atgtggtgtc gcagattatc gtgcgcgata tcaaccgtat
     5281 cagcgatttg aacgaagtaa cccgcacgat tacgctgttt gccgattttg ccgtcaatac
     5341 cgcgctggat tttgcctacg cctattatcg ggacatgtac ggcacgccga tcgggcgtta
     5401 taccaaatcg ccgcagcatt tgagcgtggt ggcgatgggc aaggcgggcg gctatgagtt
     5461 gaacgtgtct tccgacatcg atttgatttt catctatccc gaatctggcg acaccgacgg
     5521 caggcgcgaa cggggcaatc aggagttttt caccaaagtc gggcagaaac tgattgcgct
     5581 gctgaacgac atcaccgccg acgggcaggt gttccgcgtc gatatgcggc tgcggccgga
     5641 cggtgattcg ggcgcgttgg tattgagcga aaccgcgctg gagcaatatt tgattacaca
     5701 ggggcgagaa tgggaacgct acgcgtggtg caaaggtcgc gtggttacgc cgtatccgaa
     5761 cgacatcaaa gcactggtgc gcccctttgt gttccgcaaa tatctggatt acggcgcgta
     5821 tgaggcgatg cgtaagctgc accgccaaat ccgcagcgaa gtcagcaaaa aaggcatggc
     5881 ggacaacatc aaactcggcg cgggcggcat ccgcgaagtc gaatttatcg cccagatttt
     5941 ccagatgata cgcggcggac aaatgcgcgc gctgcaactg aaaggcacgc aggaaacgct
     6001 gaagaagctt gccgaaaccg ggatcatgcc gtctgaaaac gtcgaaaccc tgcttgccgc
     6061 ctaccgcttc ctgcgcgatg ttgaacaccg cctgcaatac tgggatgacc agcaaaccca
     6121 aaccctgccg acctcgcccg aacagcggca actgctcgcc gaaagcatgg gtttcgacag
     6181 ttattccgct ttttcagacg gtctcaacat tcatcggaac aaagtcaatc agttgttcaa
     6241 cgaaattttg agcgaacccg aagagcaaac gcaagacaac agcgaatggc aatgggcatg
     6301 gcaggaaaag cctgatgaag aagaacggct aggccgtctg aaagaatatg ggtttgatgc
     6361 cgaaaccatc gccacgcggc tcgaccaaat ccgcaacggc cataaatacc gccacctttc
     6421 cgcccacgcc cagccgcgtt ttgacgccat tgtgccgttg ttcgtacaag cggcggcaga
     6481 gcaaaacaac ccgaccgata cattgatgcg gctgttggat tttctcgaaa acatcagccg
     6541 ccgatccgcc tatctcgcct tcctcaacga acatccgcaa accttggcgc aactggcgca
     6601 gattatgggc caaagttctt gggtggcggc gtatctgaac aaatatccga ttttgttgga
     6661 cgaactcatc agcgcgcagc ttttggatac cgcgtttgat tggcaggcgc tcgccgccgc
     6721 cctttcagac gacctcaaag cctgcggcgg cgatactgaa gcgcaaatgg acaccctgcg
     6781 ccgcttccag cacgcccaag tcttccgtct cgccgtccaa gacctcgccg gactgtggac
     6841 ggtagaatcc ctctccgacc aactctccgc cctcgccgac accatcctcg ccgccgccct
     6901 gctgtgcgca tgggcggaca tgccaaaaaa acaccgcgac acaccgcaat tcgccatcgt
     6961 cggctacggc aaactcggcg gcaaagaact cggctacgcc tccgacctcg acttggtcta
     7021 tctttatgac gacccgcatc ccgatgcagg cgacgtgtac agccgccttg cccgccgcct
     7081 gaccaactgg ctttccaccg ccaccggcgc aggcagcctc tacgaaaccg acctgcgcct
     7141 gcgccccaat ggcgatgcag gtttcctcgc ccacagcatc gccgcctttg aaaaatacca
     7201 gcgcgaaaac gcctggacgt gggaacacca atccctcacc cgcgcccgct tcatctgcgg
     7261 cacacccgaa attcagacgg ccttcgaccg catccgcacc gaaatgctga ctgccgaacg
     7321 cgaccaaacc gccttagcag gcgaaattat cgaaatgcgc gaaaaaatgt tccccaccca
     7381 tccgcctgcc gacagcaacg tcaaatacgc gcgcggtggc gtggtcgatg tcgaatttat
     7441 cgtccaatat ctgatacttg cccatgcccg ccagtatccg caactcttgg acaactacgg
     7501 caacatcgcc ctcttaaaca tcgccgccga ctgcagcctt atcgacaaaa acctcgccga
     7561 acaaagccgc accgcctacc gcttctaccg ccggcagcag cacaacacca aactgcgcga
     7621 cgcggcaaaa accgaagtaa ccggcgaact gttggcacat tacggcaatg tcaggaaatt
     7681 gtggcgggac gtgttcggcg aagaagcggc aaccgcctga acaaaaaatg ccgtctgaag
     7741 cctgacaatc tgggtttcag acggtatttt cgtaccgtgc cgttttaagg ttgcggcaga
     7801 gctaaagcga tttatcggga atggctgaaa cccaaaaacc ggattcctct ttcgcgggaa
     7861 tgacgggatt tcaggcttct gtttttgtgg gaatgatggg attttttacc caagcaaaaa
     7921 tcaaaacaaa caaataagaa ccgtttaaaa ccccgccgtt tccattaaaa tagcgcattc
     7981 tacttttcag acg
//
LOCUS       DAXHSF010000090         7977 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.090, whole genome shotgun sequence.
ACCESSION   DAXHSF010000090 DAXHSF010000000
VERSION     DAXHSF010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7977
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.090"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            440..664
                     /locus_tag="ACFM8S_001362"
     CDS             440..664
                     /locus_tag="ACFM8S_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFC6314429.1"
                     /translation="MKVISVLNQKGGSGKTTVATHLARALQLAGNSILLVDSDPQGSS
                     RDWAAVHEEQPVPVIGIDSPTIDRDLKSVV"
     gene            680..1084
                     /locus_tag="ACFM8S_001363"
     CDS             680..1084
                     /locus_tag="ACFM8S_001363"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HFC6314430.1"
                     /translation="MDGAPQTADLAISAIKASDLVLIPVQPSPYDIWATADLVNLIKQ
                     RIKLIDGNLKAAFLINRTIKGTKIGGEIRAALEEYELPVLSTVIVQRIIYPTSAITGS
                     TVLDKEPNGEAAKEIQSLANEIINLINIDNHI"
     gene            1127..1330
                     /locus_tag="ACFM8S_001364"
     CDS             1127..1330
                     /locus_tag="ACFM8S_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HFC6314431.1"
                     /translation="MNGLKADRPSANKANLSIDDVIKQEEIKRISFDMSESLHRELKK
                     YAAENGVTIRVVLNKLVSDLLNK"
     gene            1506..2669
                     /locus_tag="ACFM8S_001365"
     CDS             1506..2669
                     /locus_tag="ACFM8S_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFC6314432.1"
                     /translation="MHINFSMEYKEFNENYLYKKPFIFKNALDVSSISWKEINELYQR
                     ADPTDWQFKFRKGEIIPKEAYVESFNDVGRIRHRFNKTAVYQYLQDGATMVYNRIDNE
                     PFVDSIAKQIAQFAQAQTVVSGYLAFGSSPSYRNHWDTRDVFAVQLIGKKHWTVSAPN
                     FDMPLYMQQAKDMPHITPPTTVDMEVILEAGDILYIPRGWWHNPMPMNCETFHLAIGT
                     FPPNGYNYMEWLMKKIPDIQSIRQNFIDWEHDQKNIDNAAQAVTEMMKNQENYQAFIQ
                     DFLGNQRVNTAFNMQIFGNLDNDRLPENSTIKLNSLDNRTIKQGYIIANGIKTNLDND
                     SQTILQWIADKHSVKLTQLYEFCQNQNINLEKVEKLVFDLTMIDVLECLTDER"
     gene            2676..2882
                     /locus_tag="ACFM8S_001366"
     CDS             2676..2882
                     /locus_tag="ACFM8S_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314433.1"
                     /translation="MFTFLNKNIFIKTIVLGCFYSFLFFSFFVSGKSFGYSFEIFNMD
                     TSALAFLSVFMGFFISVAVHIYDI"
     gene            2906..3241
                     /locus_tag="ACFM8S_001367"
     CDS             2906..3241
                     /locus_tag="ACFM8S_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HFC6314434.1"
                     /translation="MKELTINDLTLVSGGFEFGRDYIEIAPVVGTAGGVASSVRAASM
                     TGEAGAILTRSGVTGLRAGVVAIPLLAYNGTVWVNRNTKLQDNLGRLIDKVTGLDQSG
                     NDYGDGTSY"
     gene            3276..5372
                     /locus_tag="ACFM8S_001368"
     CDS             3276..5372
                     /locus_tag="ACFM8S_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HFC6314435.1"
                     /translation="MSIYLSRLSFGFSRRLLVVLQTEAAECGLACLVSVLGFHGFYTD
                     LRHLRARFSLSLKGATLADLVRFANSMNLTARAVRLDLDELVNLRLPCILHWDLNHFV
                     VLHEVHRNHIVIMNPASGWQKVRMEEVSRCFTGVALELFPNTQFEEKHETRKIRVLPM
                     LRGVVGLKRSLFQLLLLAAVLQVFALASPFFMQWVIDHAIVSADRDLLMTLALCFGLL
                     MIVRQLVSLLQTWAGMYLSTSLNIQWKANVLRRLMDLPVSYFAKRHLGDVVSRFGSVD
                     SIQSTLTSTFFVLVLNSIMAVFTLGLMYLYSPSLTTVVLVVLLIYIGIRWVAYYPLRH
                     ATQENIVHAAKQSSYFMETIRGIQTVKLFDKNAQRHAAWLNLFIDTINTGLMTQKLSA
                     LFGFTNGLLFGIANILIVYFGAVSVLNGGFTVGALMAFMSYKSQFESKAGSLIDQFVQ
                     IKMLGLHAERLADIVLEETENEQNNDFSLPKNLEHFDIQIENVSFSYAENEPYILQNF
                     SLIIKQGTAIAFAGHSGCGKSTLIQILTGSLKPESGHVLLGGHDIHALPPAFIRTWSA
                     SVMQNDVLFTGSIAENISFFDDTPNMEKIAFCAQMANIHHEIVAMPMAYETLIGDMGS
                     ALSGGQKQRIVLARALYREPKILFLDEASSHLDINNERVINDNLRQLNITKIMVAHRQ
                     ETLNTADSVVYLDKVA"
     gene            5374..6636
                     /locus_tag="ACFM8S_001369"
     CDS             5374..6636
                     /locus_tag="ACFM8S_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HFC6314436.1"
                     /translation="MTLFRPEVFQAKKNRWTGQIVLVRPFSLQFLTFFAVSLAAILVA
                     FLIFGSYTNKTTVTGQLLPTTGVVRVYSQDMGVIAHQHVMNGDFVKKGDVLFTLSTSR
                     NDNNGSIQARLLAEAELKKSLSEQEIIMKKRVHAAEKTAQENTVHRFQNQMQHVRNQI
                     IMQEKRIAISEKMLEKQRYLAKMDAISELEKNSYEIALLELKAGLAAYQREADNLARE
                     ITVQQSNLKNLPEQQATEISQLERAVSVYQQEILDYQQRNEQTIRATISGYVSSINTE
                     IGQQVDTNKLLMSIVPKESELLANLYVPSRAIGFVKPNDKVILRYQAYPYQKFGHAEG
                     HVISIAQTALGAQEWTNLGNIFTQTAQVNEPVYLIKVKLDNQHIRIYGTEKKLQIGMI
                     LEADILHENKRLYEWILDPLYQVMGKIS"
     gene            complement(6906..7745)
                     /locus_tag="ACFM8S_001370"
     CDS             complement(6906..7745)
                     /locus_tag="ACFM8S_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314437.1"
                     /translation="MGNFAKVSNCEFWDKINYNRAAQAAEQRAKQAQETPKEPPKQEE
                     HFIAKLPNDKLMTIYRLFQKQLDDMRGLERDIERGDWYINARYRMNENRNKIRALEDD
                     NKRLHHDYWAVQDQGENRFFGMGKIGAKLVKENEKQQHINERHGANVREIRRLKTENE
                     KLEGQYSDKLAADVVKVRDRFNEELDNVRREIGKRPMEEFRNVFLQAAQEAQTTIGQK
                     AVQIELEKFPLNGSPSAKRIAFYFGAQRIREVEQKEREDQRKEQERQARRMERDDGHG
                     IGM"
     gene            7786..>7977
                     /locus_tag="ACFM8S_001371"
     CDS             7786..>7977
                     /locus_tag="ACFM8S_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC6314438.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQRTR
                     YLRDHRGRPTYSLLSMFKSV"
BASE COUNT         2398 a         1448 c         1639 g         2492 t
ORIGIN      
        1 ctgattaaag tgagtgcgca aagccatctg aaggcgatgt gtttgaacct gttgaaagcg
       61 gccaacaggc taagtgcgcc cgctgccgcc taaaaggcgg cccggatgcc tgattatggg
      121 gcgtccgggg aggattaagg gggcatttgg gtaaaatcag gagtaattag gagcggaaat
      181 agacgaaaac ctgtgtttgg gtttcggctg ttgggggaag ggcttttttg caaaggtctc
      241 aatttaaact ttaacgcaat atcaactcat tttctgccac atatctcaaa aaagtctagt
      301 ttgatacagt aatctcttta acagcttttc ttgctaaaat ttaaaacaaa atgtaaatat
      361 tcttttattt atttaaataa tagattaaaa tattatttaa ttaaaagcat aatgagcgta
      421 gatattcaaa ggagctaaca tgaaagttat ttccgtatta aatcaaaagg gtggaagtgg
      481 taaaaccaca gtcgcaactc acttggcacg cgccttgcaa ttagcaggaa atagcatctt
      541 attggttgat agcgatccac aaggaagcag tagggactgg gctgctgttc acgaagagca
      601 acctgttcct gtgattggta ttgacagccc gacaattgat cgtgatttaa aaagcgttgt
      661 ttaaaaagat tttgttatta ttgatggcgc accacaaaca gccgatttag ccatatcagc
      721 gattaaagcc tctgatttag ttttaatccc tgttcaaccg tcgccatacg atatttgggc
      781 aaccgctgat ttggttaatt taatcaagca gcggataaaa ctcatagatg gaaatttaaa
      841 ggctgctttt ttaattaacc gaactattaa aggaacaaag attggcggag aaattagagc
      901 agcgttagaa gaatatgagt tacctgtttt atcaaccgtg attgtgcaac gtattattta
      961 tccaacatct gctatcacag gtagcactgt actggataaa gaaccaaatg gagaagcagc
     1021 taaggaaatt caatcacttg cgaatgagat aataaatttg ataaatatag ataatcatat
     1081 ttaatgaaat attattttaa atttttaaat attgtaggaa ataattatga acggattaaa
     1141 agcagatcgt cctagtgcaa acaaagcaaa cttatctatt gatgatgtga tcaagcaaga
     1201 agaaatcaag agaatcagtt ttgatatgtc tgaatctctg catagagaat tgaagaaata
     1261 cgcggctgag aatggtgtaa ctattcgcgt cgtgctcaat aaactcgttt cagatttatt
     1321 gaataaataa taacacacgg cagaatgtat ttgaccataa tgtcaaagca atatccaact
     1381 ttatctaaaa ataacaaaat accttcttcc aaaaacagaa cgtcttttca ttgcaaaact
     1441 tgacatataa acactagtta agtaaactta attttcatta ttaataatga aaataaggat
     1501 tatttttgca tattaacttt tcaatggaat ataaagaatt taatgaaaat tatttatata
     1561 aaaagccatt tatttttaaa aatgctttag atgttagttc tatctcatgg aaagaaatta
     1621 atgagttgta tcaacgtgca gaccctactg attggcaatt taaatttcgc aaaggggaaa
     1681 taatccccaa agaagcctat gttgaatcat tcaatgatgt gggtagaata cgtcatcggt
     1741 ttaataaaac agccgtatat cagtatctgc aagatggtgc aacaatggtt tataaccgta
     1801 ttgataatga gccatttgtt gacagtattg ctaaacaaat tgcccagttt gctcaagcac
     1861 aaactgttgt tagtggttat ttggcgtttg gtagttctcc atcatatcgt aatcattggg
     1921 atactcgtga tgtgtttgcg gttcaattga ttggcaaaaa acactggaca gtttctgctc
     1981 caaattttga tatgccattg tatatgcagc aagcaaaaga tatgccgcac attactccgc
     2041 ctacaacagt agatatggaa gtcattttag aagcaggaga tattttatat atcccgcgtg
     2101 gttggtggca taatcctatg cctatgaatt gtgaaacatt ccatttagct attggaactt
     2161 ttccgccaaa tggttataat tatatggaat ggttgatgaa aaaaatacct gatatccaga
     2221 gtattcgtca aaatttcata gactgggaac acgaccaaaa aaatatagat aatgcggctc
     2281 aggcagttac tgaaatgatg aagaaccaag aaaattatca agcattcata caagattttt
     2341 tgggaaatca acgcgtaaat actgcattta atatgcaaat ttttggaaat ctggataatg
     2401 acagattacc tgaaaatagt acaattaaac tcaattcgtt ggataaccgt acaattaaac
     2461 aaggatatat catagcgaat ggcatcaaaa ccaacttgga caatgatagc caaacgattt
     2521 tgcaatggat tgctgacaaa catagtgtta agttaactca actgtatgaa ttttgccaaa
     2581 atcaaaacat taatttggaa aaagtggaaa aattagtgtt tgatttgaca atgatcgatg
     2641 tattggaatg tttaactgat gaaagataaa ttattatgtt tactttttta aataaaaata
     2701 tatttatcaa aacaattgta cttggctgtt tttattcctt tttatttttt agtttttttg
     2761 tatctggtaa gtcatttggt tattcatttg aaatttttaa tatggataca agtgcgcttg
     2821 cttttttaag tgtatttatg ggctttttta taagcgtggc tgtgcatatt tacgatattt
     2881 aatacaattg aaaagataaa taattatgaa agaattaact attaatgatt taaccctagt
     2941 ttcgggtgga tttgaatttg gtcgtgatta tattgaaata gctccagttg taggtacagc
     3001 aggtggcgtt gcctcttctg tgagagcagc ttcaatgact ggagaagctg gagcaattct
     3061 tactcgttca ggagtaacag gattacgtgc tggagtggta gctattccat tgttagccta
     3121 caatggtact gtatgggtta atagaaatac caaattacaa gataatttag gtagattaat
     3181 agataaagta actggcctgg atcaatcagg caatgattat ggcgatggga caagttatta
     3241 atctcataat actttaaaat caaaaaagaa caccaatgtc aatatatcta tctcgcctat
     3301 cattcggctt ttctcgccgt ttactagttg tcttgcaaac ggaagcagca gaatgtggat
     3361 tggcgtgttt ggtatcggta ttggggtttc atggttttta tacggatttg cgccatttaa
     3421 gggcgcgttt ttctttgtct cttaaaggcg caacattggc agatttggta cggtttgcta
     3481 atagcatgaa tttaactgct cgagctgttc ggttggattt agatgaattg gtaaatttgc
     3541 gcttaccctg tattttacat tgggatttaa atcattttgt ggtgttgcat gaagttcatc
     3601 gtaatcacat tgtgattatg aaccctgctt cgggttggca aaaagtgcgt atggaagaag
     3661 tctcgcgctg ttttacaggt gtagcgttgg aactatttcc taacacgcaa tttgaagaga
     3721 aacacgaaac gcgtaaaatt cgcgtactac cgatgttgcg cggagtggtg ggattaaaaa
     3781 ggtctttatt tcagcttctg ctgctggcgg cagtattaca ggtgtttgct ttggcaagcc
     3841 ctttttttat gcaatgggta atagaccatg ccattgtatc ggcagaccgt gatttattga
     3901 tgacattggc attgtgtttt ggcttgttga tgattgtgcg gcagttagtg tcattgttgc
     3961 aaacttgggc gggtatgtac ttatctacct ctttgaatat tcaatggaaa gctaatgtat
     4021 tacgccgctt aatggatttg cctgtttctt atttcgctaa acgacatttg ggcgatgtgg
     4081 tttcacgttt tggctcagtg gacagtattc aaagtacgct cacttctact ttctttgtgt
     4141 tggtgctcaa cagcattatg gcggtattta cattagggtt gatgtatttg tatagcccaa
     4201 gtttaacgac agttgtactg gttgttttgc tgatttatat tggtattcgt tgggtggcat
     4261 attatccatt acgccatgca actcaagaaa atattgttca tgctgccaag cagagcagtt
     4321 atttcatgga aaccatacgt ggtattcaaa ctgttaaatt atttgataaa aatgcacaac
     4381 gccatgcggc ttggctgaac ttgtttattg acacgattaa cacaggttta atgacacaaa
     4441 aactttccgc gctgttcggt tttacgaatg gccttttgtt tggtatcgct aatattttaa
     4501 ttgtgtattt tggtgcggtt tctgtattga acggtggttt taccgttggg gcgttaatgg
     4561 cgtttatgtc ctacaaaagt cagtttgaga gtaaagcagg ttcattgatt gaccagtttg
     4621 tacaaatcaa aatgctagga ttacacgcgg aacgcttggc ggatattgtg ctagaagaaa
     4681 cggaaaatga acaaaacaac gatttcagtc tgcctaaaaa tttagaacat tttgatattc
     4741 aaatagaaaa tgtatcattt agctatgctg aaaatgaacc atacattttg caaaatttca
     4801 gtttaatcat caaacaagga acagccattg cgtttgctgg acattcaggc tgtgggaaat
     4861 ctacgctcat tcaaattctg acaggcagtc tgaaacctga aagcggtcat gtgttattag
     4921 gtgggcatga cattcatgcg ttaccacctg catttatccg cacatggagc gcaagtgtga
     4981 tgcaaaatga tgtgttattt actggctcaa tcgcagaaaa catcagtttt tttgatgata
     5041 cgcccaatat ggaaaaaatc gcattttgcg ctcaaatggc gaatattcat catgaaattg
     5101 tcgctatgcc tatggcgtat gaaacattga ttggcgacat ggggagtgca ttgagtggtg
     5161 ggcaaaagca gcgcatcgta ctagctcgtg ctttatatcg tgaaccaaaa attttgtttt
     5221 tagatgaagc cagtagccat ttggacatca acaatgaacg tgtgattaac gataatctac
     5281 gccaactcaa cattaccaaa atcatggtcg cgcatcgtca ggaaacttta aatactgctg
     5341 atagcgtagt ttatttagac aaggtagcct gaaatgacat tatttcgccc cgaagtattc
     5401 caagctaaaa aaaatcgctg gacagggcaa attgttttag tgcgtccttt ttccttgcag
     5461 tttttaacat ttttcgcggt ctcattagcg gctattttag ttgctttttt aatttttggg
     5521 agctatacca ataaaaccac cgtaacagga caacttttgc cgacaacagg ggtagtacgc
     5581 gtatattcgc aggatatggg ggttattgct catcaacacg ttatgaatgg cgattttgtg
     5641 aaaaaaggcg atgtgttatt tacattatct acttcacgaa atgacaataa tggcagcata
     5701 caagcacgat tactcgccga agctgaatta aaaaaatcac tatcagaaca agaaattatt
     5761 atgaaaaaac gtgttcatgc agcagaaaaa accgcacaag aaaacacggt acaccgtttt
     5821 cagaaccaaa tgcaacacgt tagaaatcaa attatcatgc aagaaaaacg tattgcgatt
     5881 tctgaaaaaa tgttagaaaa acagcgttat ttagccaaaa tggacgcaat ttctgaatta
     5941 gaaaaaaata gttatgaaat tgctttattg gagctgaaag caggtttagc cgcttaccaa
     6001 cgagaagcag ataatcttgc tcgggaaata actgtacagc aaagcaatct aaaaaatctg
     6061 cctgaacagc aagctactga aatcagtcaa ttagaacgcg cagtatctgt ctatcaacaa
     6121 gaaatattgg attatcaaca acgtaacgaa caaactattc gcgcaaccat atcaggctat
     6181 gtcagttcta ttaacacgga aattggtcag caggtagata ctaacaaact attgatgagt
     6241 atcgtgccga aagaaagtga attactggct aatttatatg tccctagtcg cgcaatcggt
     6301 tttgtaaaac caaatgacaa ggtaattttg cgttatcaag catatcctta tcaaaaattc
     6361 ggacacgcag aaggacacgt catttctatt gctcaaacgg cactaggggc gcaagaatgg
     6421 acaaatttag gcaatatttt tactcaaacg gcacaagtga atgagcctgt ttatttaatc
     6481 aaagtaaaac tggataatca gcatattcgt atatatggca cggagaagaa attgcaaatt
     6541 ggtatgatac tagaagccga tattttgcat gagaataaac ggttatatga atggatactt
     6601 gaccctttgt atcaagtaat gggaaaaata tcatagctga attgctcaat acattggagc
     6661 gtatgagtgc ccctaattat caagcaaaac caatgtccga tgacttccaa tttgttgtta
     6721 agcacaaata cacagtgttt tatgtaataa tgaagacaga gaagtgatac aatttttcgc
     6781 tccatttttg cttcctgaat tgctgcaatt gtatctgcgt tgtatgcgag aagtaaatat
     6841 tttcgatgca tttgctgact ttgtaccata atacgtcttc caaccagtat gataggctgg
     6901 tttttctaca tgccgatgcc gtggccgtcg tcccgctcca tacgccgcgc ctgccgctcc
     6961 tgctctttgc gctggtcttc ccgctccttc tgctctactt cccttatccg ctgcgcgccg
     7021 aaataaaacg cgattcgctt ggccgaaggg ctgccgttca gggggaattt ttccagttcg
     7081 atttgaaccg ccttctgccc tattgtcgtc tgtgcttctt gggctgcctg caaaaatacg
     7141 ttcctgaatt cttccatcgg ccgtttgccg atttccctgc ggacattgtc cagttcttcg
     7201 ttaaacctgt cgcgtacttt gaccacgtcg gcggccaact tgtcgctata ctgcccttcc
     7261 agtttttcgt tttctgtttt cagccgccgg atttctcgca cgttcgctcc atgccgttcg
     7321 ttgatgtgtt gctgcttctc gttttcctta accagttttg cgccgatttt acccatgccg
     7381 aaaaagcggt tttcgccttg gtcttggact gcccaatagt cgtgatgcag ccgtttgttg
     7441 tcgtcttcca gcgcccttat tttgtttcgg ttttcgttca tgcggtatcg cgcattgatg
     7501 taccaatctc cgcgttctat atcgcgctcc aagccgcgca tgtcgtccag ttgtttttga
     7561 aacagcctgt atatcgtcat tagcttgtcg ttcggcagct tggcgataaa gtgctcttcc
     7621 tgtttcggcg gctctttcgg tgtttcctgc gcctgctttg cccgctgctc ggcggcttgg
     7681 gcagcccgat tataattaat tttatcccag aattcacaat ttgagacctt tgcaaaattc
     7741 cccaaaatcc cctaaattcc tactaagaca tttaggggat ttctcatgag taccttcttc
     7801 cagcaaaccg cacaagccat gattgccaaa cacatcgacc gcttcccact attgaagttg
     7861 gatcaggtga ttgattggca accgatcgaa cagtacctga atcgtcaaag aacccgttac
     7921 cttcgagacc accgaggccg tcccacctat tccctgttgt ccatgttcaa atccgtc
//
LOCUS       DAXHSF010000091         7964 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.091, whole genome shotgun sequence.
ACCESSION   DAXHSF010000091 DAXHSF010000000
VERSION     DAXHSF010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7964
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.091"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            185..1354
                     /gene="prpF"
                     /locus_tag="ACFM8S_001372"
     CDS             185..1354
                     /gene="prpF"
                     /locus_tag="ACFM8S_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HFC6314439.1"
                     /translation="MPQIKIPAVYYRGGTSKGVFFKRSDLPEAAREAGSARDKILLRV
                     LGSPDPYGKQIDGLGNASSSTSKAVILDKSERADHDVDYLFGQVSIDKPFVDWSGNCG
                     NLTAAVGVFSIEQGLVDKGKIPSDGICTVKIWQKNIGKTIIAHVPMQNGAVLETGDFE
                     LDGVTFPAAEVQIEFLDPADGEGSMFPTGNLVDEIDVPNIGRLKATLINAGIPTVFLN
                     AADLGYTGKELQDDINNDAAALEKFEKIRAYGALKMGLISDVSEAAARAHTPKVAFVA
                     PAADYTASSGKTVNAADIDLLVRALSMGKLHHAMMGTASVAIATAAAVPGTLVNLAAG
                     GGTRKEVRFGHPSGTLRVGAAAECQDGQWTATKAVMSRSARVMMEGWVRVPEDCF"
     gene            1768..2967
                     /locus_tag="ACFM8S_001373"
     CDS             1768..2967
                     /locus_tag="ACFM8S_001373"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFC6314440.1"
                     /translation="MSDQLILVLNCGSSSLKGAVIDRKSGSVVLSCLGERLTTPEAVI
                     TFNKDGNKRQVPLSGRNCHAGAVGMLLNELEKHGLHDRIKAIGHRIAHGGEKYSESVL
                     IDQAVMDELNACIPLAPLHNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTY
                     AVPRELRKKYAFRRYGFHGTSMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAIKN
                     GKSVDTSMGFTPIEGLVMGTRCGDIDPGVYSYLTSHAGLDVAQVDEMLNKKSGLLGIS
                     ELSNDCRSLEIAADEGHEGARLALEVMTYRLAKYIASMAVGCGGVDALVFTGGIGENS
                     RNIRAKTVSYLDFLGLHIDTKANMEKRYGNSGIISPTDSSPAVLVVPTNEELMIACDT
                     AELAGIL"
     gene            3075..3695
                     /locus_tag="ACFM8S_001374"
     CDS             3075..3695
                     /locus_tag="ACFM8S_001374"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFC6314441.1"
                     /translation="MAQFFAIHPDNPQERLIKQAVEIVNQGGVVVYPTDSCYALGCKL
                     GDKAAMERILSIRKIDLKHHLTLMCADLSELGTYAKIDNVQFRQLKAATPGPYTFILQ
                     ATKEVPARTLHPKRKTIGLRIPDNAIAQALLGELGEPLLSCTLMLPEDGEPLTDPYEI
                     RECLEHAVDLVIDGGWCGTEPTTVVDMTDGTELVRQGCGDTAVFGL"
     gene            complement(3822..4265)
                     /locus_tag="ACFM8S_001375"
     CDS             complement(3822..4265)
                     /locus_tag="ACFM8S_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFC6314442.1"
                     /translation="MYLTQHTDYGLRVLIYTAINDDALVNISTIAGTYGISKSHLMKV
                     VTALVKGGFLHSVRGKGGGLRLAAPPDRINIGSVVRHLEPMQLVECMGENNECLITPS
                     CRLTGILGGAMKSFFTYLDGFTLQDLLNKPTYDLLYEPRIPIAVQ"
     gene            4439..4807
                     /locus_tag="ACFM8S_001376"
     CDS             4439..4807
                     /locus_tag="ACFM8S_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HFC6314443.1"
                     /translation="MKPLKRHPALIGLSRDHHHSLSLCVRLLRTPEERHRDELEPHFA
                     ELETHFREEEAKFAPIWQNVAPELKQRFEKDHARLRQMMASPEYGNAAWNTAFATTLR
                     DHARFEERELFPAAEPFLPA"
     gene            4852..6006
                     /locus_tag="ACFM8S_001377"
     CDS             4852..6006
                     /locus_tag="ACFM8S_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HFC6314444.1"
                     /translation="MKFTKHPVWAMAFRPFYSLAALYGALSVLLWGFGYTGTHELSGF
                     YWHAHEMIWGYAGLVVVAFLLTAVATWTGQPPTRGGVLVGLTIFWLAARIAAFIPGWG
                     ASASGILGTLFFWYGAVCMALPVIRSQNQRNYVAVFALFVLGGTHAAFHVQLHNGNLG
                     GLLSGLQSGLVMVSGFIGLIGTRIISFFTSKRLNVPQIPSPKWVAQASLWLPMLTAML
                     MAHGVMPWLSAAFAFAAGVIFTVQVYRWWYKPVLKEPMLWILFAGYLFTGLGLIAVGA
                     SYFKPAFLNLGVHLIGVGGIGVLTLGMMARTALGHTGNPIYPPPKAVPVAFWLMMAAT
                     AVRMVAVFSSGTAYTHSIRTSSVLFALALLVYAWKYIPWLIRPRSDGRPG"
     gene            complement(6063..6935)
                     /locus_tag="ACFM8S_001378"
     CDS             complement(6063..6935)
                     /locus_tag="ACFM8S_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFC6314445.1"
                     /translation="MPILNHIALIGVGLIGGSFVLDLKRQGLVRTVTGIDTDRDNLER
                     ALERGVIDRASVAIDADSIGGADLVLIATPVATFPAILTALRPVLPEHTWISDVGSTK
                     SSVIEAFRRCLPDRLHRCIAAHPIAGSDRSGAQAAQFGLFRHRKLIITPHGGEHSDGI
                     ALVEELWHAVGAEIYTMDAQRHDAVFAAVSHMPHLTAFAYVHQILDHPDGQEYLKFAA
                     TGFRDFTRIASGHPAVWADICLANKDSLLQLVQGLGKQLDVLADILTTDDREALYRYF
                     EEAKTTRDRWLDGN"
     gene            7020..7832
                     /locus_tag="ACFM8S_001379"
     CDS             7020..7832
                     /locus_tag="ACFM8S_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HFC6314446.1"
                     /translation="MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGVDWVLLPEY
                     WVLMGANDTDKLALAEPLGGGRFQTALSETAKECGVVLFGGTVPLQSPEAGKVMNTLL
                     VYGRDGKRTGLYHKMHLFGFSGLGERYAEADTIRAGGDVPHLSAEGVSVAAGICYDVR
                     FPEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRARAVENQCYVVAAAQGGLHENGRR
                     TFGHSMIVDPWGDVLDVLPEGEGIVTADIDANRLNSVRNRLPALKYRVLDAV"
BASE COUNT         1716 a         2188 c         2312 g         1748 t
ORIGIN      
        1 aaatttggat tcccgcctgc gcgggaatga cggggtttaa taatctgccg tatcacaaca
       61 cagtagccgt agattgtggc gaaccccgac agtttgcgga atcaaacggc tttggtcgga
      121 gtggcagcct aatccactat aaaaatcgtg ggcagagccc acgctacata aggagaatct
      181 agaaatgccg caaattaaaa tccccgccgt ttactaccgc ggtggcacat caaaaggcgt
      241 gtttttcaaa cgttccgatc tgcctgaggc ggcgcgggaa gcgggtagcg cgcgcgacaa
      301 aatcctgttg cgcgtactcg gtagccccga cccttatggt aaacagattg acggtttggg
      361 caatgccagt tcgtccacca gcaaagccgt gattttggac aagtccgaac gcgccgatca
      421 cgatgtcgat taccttttcg ggcaagtttc catcgacaaa ccttttgtcg attggagtgg
      481 caactgcggc aacctcaccg ctgccgtggg cgtattctcc atcgaacagg gcttggtcga
      541 taaaggcaag attccttcag acggcatctg cacggtcaaa atctggcaga aaaacatcgg
      601 caaaaccatt attgcccatg taccgatgca aaacggcgca gttttggaaa caggcgattt
      661 tgagctcgac ggcgtaacgt tcccggcagc cgaagtacaa atcgaatttc ttgatccagc
      721 cgacggcgaa ggcagtatgt tcccaaccgg caatttggtc gatgaaattg atgtgccgaa
      781 tataggccgt ttgaaagcca cgctcatcaa cgcgggcatt ccgaccgttt tcctgaatgc
      841 cgccgacttg ggctacacgg gcaaagagtt gcaagacgac atcaacaacg atgccgcagc
      901 tttggaaaaa ttcgagaaaa tccgcgctta cggtgcgctg aaaatgggtc taatcagcga
      961 cgtatccgaa gctgccgccc gcgcgcacac gccgaaagtc gccttcgtcg cgcccgccgc
     1021 cgattacacc gcctccagtg gcaaaaccgt gaatgccgcc gacatcgatt tgctggtacg
     1081 cgccctgagc atgggcaaat tgcaccacgc gatgatgggt accgcctctg ttgccattgc
     1141 gaccgccgcc gccgtgcccg gtacgctggt caaccttgcc gcaggcggcg gaacgcgtaa
     1201 agaagtgcgc ttcgggcatc cttccggcac attgcgcgtc ggtgcagccg ccgaatgtca
     1261 ggacggacaa tggacggcca ccaaagcggt tatgagccgc agcgcacgcg tgatgatgga
     1321 aggttgggtc agggtgcctg aggattgttt ttaaattgac gtagcatggg tttgcccgcg
     1381 agccataaaa aggtcgtctg aaaaacaagt aaacatcaaa tcactgacca ttcccttccc
     1441 ttgccctgtg gcggaaggcg gcaaatcaca aggaagaaca cggaaacccc gataaaagac
     1501 agcttcccgt attaccgtca ttcccgcgca ggcgggaatc cagacctgtc aatatggagg
     1561 attggtaggg gaaaacaggt ttcgtgagtt ctacattctg gattcccgcc acagcctgtc
     1621 ctcgcgtagg cggggacgga ataacgatag aaaatgcggc atacgctttg cccaaagagg
     1681 ccgtctgaaa caccttgcgc ctgatgtctg cctttttcag acgaccccac accaaaaaaa
     1741 caaccacaaa ctacaaggag aaacatcatg tccgaccaac tcatcctcgt tctgaactgc
     1801 ggcagttcat cgctcaaagg cgccgttatc gaccgaaaaa gcggcagcgt cgtcctaagc
     1861 tgcctcggcg aacgcctgac cacgcccgaa gccgtcatta cgttcaacaa agacggcaac
     1921 aaacgccaag ttcccctgag cggccgaaat tgccacgccg gcgcggtggg tatgcttttg
     1981 aacgaactgg aaaaacacgg tctgcacgac cgcatcaaag ccatcggcca ccgcatcgcc
     2041 cacggcggcg aaaaatacag cgagtctgtt ttgatcgacc aggccgtaat ggacgaactc
     2101 aatgcctgca ttccgcttgc gccgctgcac aaccccgcca acatcagcgg catccttgcc
     2161 gcacaggaac atttccccgg tctgcccaat gtcggcgtga tggatacttc gttccaccaa
     2221 accatgccgg agcgtgccta cacttatgcc gtgccgcgcg agttgcgtaa aaaatacgct
     2281 ttccgccgct acggtttcca cggcaccagt atgcgttacg ttgcccctga agccgcacgc
     2341 atcttgggca aacctttgga agacatccgc atgattattg cccacttagg caacggcgca
     2401 tccattaccg ccatcaaaaa cggcaaatcc gtcgatacca gtatgggttt cacgccgatc
     2461 gaaggtttgg taatgggtac acgttgcggc gacatcgatc cgggcgtata cagctatctg
     2521 acttcacacg ccggtttgga tgttgcacaa gttgatgaaa tgctgaataa aaaatcaggc
     2581 ttgctcggta tttccgaact ctccaacgac tgccgttctt tggaaatcgc tgcggatgaa
     2641 ggccatgaag gcgcgcgcct cgccctcgaa gtcatgacct accgcctcgc caaatacatc
     2701 gcttcgatgg ctgtgggctg cggcggcgtt gacgcactcg tgttcaccgg cggtatcggc
     2761 gaaaactcgc gtaatatccg tgccaaaacc gtttcctatc ttgatttctt gggtctgcac
     2821 atcgacacca aagccaatat ggaaaaacgc tacggcaatt cgggcattat cagcccgacc
     2881 gattcttctc cggctgtttt ggttgtcccg accaatgaag aactgatgat tgcctgcgac
     2941 actgccgaac ttgccggcat cttgtagcca aaaaagggac gagtccgcaa aaatgccgtc
     3001 tgaaattttc agacggcatt ttgtttggcg tttgggcgta caatacggaa aacatgacga
     3061 taaggaaaca aaccatggca cagtttttcg ctattcatcc cgacaatccc caagaacgcc
     3121 tcatcaagca ggcggttgaa atcgtcaatc aaggtggcgt ggtggtttat ccgaccgatt
     3181 cgtgttacgc cttgggttgc aaactcggcg ataaggcggc gatggaacgc atactctcca
     3241 tccgcaaaat cgatttgaaa caccacctga ccctgatgtg cgccgatttg agcgagttgg
     3301 gcacgtacgc caaaatcgac aacgtacagt ttcgtcagct taaggccgcc acgcccggac
     3361 cttatacctt tatcttgcag gcgacgaagg aagtgccggc gcgcacgctg cacccgaaac
     3421 gcaaaaccat cgggctgcgt attcccgata atgccattgc acaagccctg ctgggggaat
     3481 tgggcgaacc gcttttaagc tgcaccctga tgctgcccga agacggcgaa ccattgaccg
     3541 atccttatga aatccgcgag tgtttggaac acgccgtcga tttggtgatt gacggcggct
     3601 ggtgcggaac cgagccgacc accgtcgtcg atatgaccga cggcacggaa ttggtgcgcc
     3661 aaggttgcgg cgatacggcg gtgttcggtt tgtagggaaa ccgatgccgt ctgaagcatc
     3721 ggctgttcag acggcattgc gcgccttgcc ggcggcagtc cgaaatgccg gcgcgtatcg
     3781 cgctcggtcg gaatatccgt ttgaaacggc attttgatgc attactgcac cgcaatcgga
     3841 attctcggtt cgtagagcag gtcgtaggtc ggcttgttga gtaggtcttg gagcgtgaaa
     3901 ccgtccagat acgtgaaaaa cgacttcatc gcgccgccga gtatgcctgt cagacggcag
     3961 gacggtgtga tcaggcattc gttgttctcg cccatgcact cgaccagctg catcggttcg
     4021 aggtggcgga cgaccgagcc gatgttgatg cggtcgggcg gtgcggcaag ccgcagaccg
     4081 ccgccttttc cgcgcacgct gtggaggaag ccgcctttga ccagcgcggt aacgactttc
     4141 atcagatggc ttttggaaat gccgtaagtt ccggcgatgg tactgatgtt gaccagcgca
     4201 tcgtcgttga tggcagtgta gataaggacg cgcagcccgt agtccgtatg ttgtgtcaaa
     4261 tacatgattt tctcggtatg gattgttatt cttatcggta cggtttaagg ttcacggaca
     4321 ataccttaat ggttgaaacc ctgtccgtcg gggcggtaga atgcagcctg tctgcggcgg
     4381 tatgccgtct gaaacatccg cgctaccgtt tgataatttg ttattgtaac tcaaaatcat
     4441 gaaaccgttg aaacgacatc ccgcccttat cgggctttcg cgtgaccacc accattcgct
     4501 ttccctgtgc gtgcgtctgt tgcggacgcc ggaagaaagg catcgggacg aactcgaacc
     4561 gcattttgcc gaattggaaa cccattttcg cgaagaagaa gccaagtttg ccccaatttg
     4621 gcagaatgtc gcccccgaat tgaaacaacg tttcgagaaa gaccacgccc gactgcggca
     4681 gatgatggca agccccgaat acggtaacgc ggcgtggaat accgcttttg ccacaaccct
     4741 gcgcgaccac gcgcgctttg aagaacgcga gctgtttccc gccgccgaac cgtttttgcc
     4801 ggcatgattc cgttttgcgg caaatatatt aatgataaac aaggaacaca catgaaattt
     4861 accaagcacc ccgtctgggc aatggcgttc cgcccatttt attcgctggc ggctctgtac
     4921 ggcgcattgt ccgtattgct gtggggtttc ggctacacgg gaacgcacga gctgtccggt
     4981 ttctattggc acgcgcatga gatgatttgg ggttatgccg gactggtcgt cgtcgccttc
     5041 ctgctgaccg ccgtcgccac ttggacgggg cagccgccca cgcggggcgg cgttctggtc
     5101 ggcttgacta tcttttggct ggctgcgcgg attgccgcct ttatcccggg ttggggtgcg
     5161 tcggcaagcg gcatactcgg tacgctgttt ttctggtacg gcgcggtgtg catggctttg
     5221 cccgttatcc gttcacagaa tcaacgcaac tatgttgccg tgttcgcgct gttcgtcttg
     5281 ggcggcacgc atgcggcgtt ccacgtccag ctgcacaacg gcaacctagg cggactcttg
     5341 agcggattac agtcgggctt ggtgatggtg tcgggtttta tcggtctgat tggtacgcgg
     5401 attatttcgt tttttacgtc caaacggttg aatgtgccgc agattcccag tccgaaatgg
     5461 gtggcgcagg cttcgctgtg gctgcccatg ctgaccgcca tgctgatggc gcacggcgtg
     5521 atgccttggc tgtcggcggc tttcgcgttt gcggcaggcg tgatctttac cgtgcaggtg
     5581 taccgctggt ggtataaacc cgtgttgaaa gagccgatgc tgtggattct gtttgccggc
     5641 tatctgttta ccggattggg gctgattgcg gtcggcgcgt cttatttcaa acccgctttc
     5701 ctcaatctgg gtgtgcatct gatcggggtc ggcggtatcg gcgtgctgac tttgggcatg
     5761 atggcgcgta ccgcgcttgg tcatacgggc aatccgattt atccgccgcc caaagccgtt
     5821 cctgtcgcat tttggctgat gatggcggca actgccgtcc gcatggttgc cgtattttct
     5881 tccggtacag cctacacgca cagcatacgc acctcttcgg ttttgtttgc acttgcgctt
     5941 ttggtgtatg cgtggaaata cattccgtgg ctgattcgtc cgcgttcgga cggcaggccc
     6001 ggttgagaca aaccgccgca gatttcgggt ctgggcggtt tgcttttcag acggcagggc
     6061 ggtcagttgc cgtccagcca gcggtcgcgt gtggttttgg cttcttcaaa atagcggtac
     6121 agggcttcgc ggtcgtcggt ggtcaggatg tctgccaaaa cgtccaactg tttgcccaag
     6181 ccttgaacca gttgcagcag gctgtctttg ttggcaaggc agatgtccgc ccacacggcg
     6241 ggatgaccgg aggcgatgcg ggtgaagtcc cgaaagcccg tggcggcgaa tttcagatat
     6301 tcctgtccgt cggggtggtc gagaatctgg tggacatagg cgaaggcggt caggtggggc
     6361 atatgggaga cggcagcgaa aaccgcgtcg tggcgttgcg cgtccatcgt ataaatttcc
     6421 gcaccgaccg cgtgccacag ttcttctacc aaggcaatgc cgtctgaatg ttcgccgccg
     6481 tgtggcgtga tgatgagttt tctgtggcgg aacagcccga actgcgcggc ttgcgcaccg
     6541 cttctgtccg aaccggcaat cgggtgggcg gcgatgcagc ggtgcaggcg gtcgggcaga
     6601 cagcggcgga aggcttcgat gaccgaagat ttggtgctgc cgacatcgga aatccaagtg
     6661 tgttccggca aaacggggcg cagcgcggtc aaaatggcgg gaaaggtggc gacgggcgtg
     6721 gcaatcagta ccaagtccgc accgccgatg ctgtccgcgt cgatggcaac ggaagcccgg
     6781 tcaatcacgc cgcgttccaa tgcacgttcg aggttgtcgc ggtcggtgtc gataccggta
     6841 acggtgcgga cgagtccctg ccttttgagg tcgagaacga acgaaccgcc gatcagccct
     6901 acaccgatga gggcaatatg gttcaaaatg ggcatttgtg taaacggttt tcgcaaagta
     6961 ccgtcatggt agcctatcgg cggaatatgc cgcaaggtcg gcaggaaaaa ggagaagaaa
     7021 tggacaaaat cagagttgcc gccgtgcaga tggtgtcggg cgtgtcgccg gaaaccaacg
     7081 tcgccgccat gaaacgcctg gtcgcacggg cggcggagca gggtgtggat tgggtgctgc
     7141 tgcccgaata ttgggtgctg atgggcgcaa acgataccga caaactcgcg cttgccgagc
     7201 ctttgggcgg cggacgcttt cagacggcat tgagcgaaac ggcgaaagaa tgcggcgtgg
     7261 tgctgttcgg cggaaccgtg ccgctgcaaa gccccgaggc gggcaaggtc atgaatactt
     7321 tattggtgta cgggcgcgac ggcaaacgga cggggctgta ccacaaaatg cacctcttcg
     7381 gcttttccgg tttgggcgaa cgctatgccg aagccgatac catccgcgcg ggcggggatg
     7441 tgccgcactt gtcggcagaa ggcgtgtcgg tggcggcggg catttgttac gatgtccgct
     7501 ttcccgaatt tttccgccgc cagctgccgt ttgacgtatt gatgctgccc gctgcgttta
     7561 cgcacacgac gggcaaggcg cattgggagc tgctgctgcg cgcgcgtgcc gtcgaaaacc
     7621 aatgttacgt cgtggcggcg gcacagggcg gtttgcacga aaacggacgg cgcacgttcg
     7681 gacacagcat gattgtcgat ccgtggggcg acgtattgga cgtattaccc gaaggcgaag
     7741 gcatcgttac ggcagacatc gatgccaacc gcctgaacag cgtccgcaac cgcctgcccg
     7801 ccttgaaata ccgggttttg gatgccgtct gaaggttcag acggcatcgg tgccggggaa
     7861 tcagcgggct gtaggatggg cttcagcctg ccatttccat caatcccaca tttctaccgt
     7921 tttcatcgaa tccatcgaat ccgccccttt cgacgaccca gtcc
//
LOCUS       DAXHSF010000092         7934 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.092, whole genome shotgun sequence.
ACCESSION   DAXHSF010000092 DAXHSF010000000
VERSION     DAXHSF010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7934
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.092"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(107..1030)
                     /gene="era"
                     /locus_tag="ACFM8S_001380"
     CDS             complement(107..1030)
                     /gene="era"
                     /locus_tag="ACFM8S_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004286080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFC6314447.1"
                     /translation="MDIETFLAGERAAGGYRCGFVAIVGRPNVGKSTLMNHLIGQKIS
                     ITSKKAQTTRNRVTGIYTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDVV
                     VFVVEAMRFTDADRVVLKQLPKHTPVILVVNKIDKDKAKDRYALEAFVAQVRAEFEFA
                     AAEAVSAKHGLRIANLLELLKPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLG
                     EELPYAMNVEVEQFEEEDGLNRIYIAVLVDKESQKAILIGKGGERLKKISTEARLDME
                     KLFDTKVFLKVWVKVKSGWADDIRFLRELGL"
     gene            complement(1077..1796)
                     /gene="rnc"
                     /locus_tag="ACFM8S_001381"
     CDS             complement(1077..1796)
                     /gene="rnc"
                     /locus_tag="ACFM8S_001381"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFC6314448.1"
                     /translation="MKDDVLKQQAHAAIQKRLGYAFRDISLLRQALTHRSHHAKHNER
                     FEFVGDSILNYTVARMLFDTFPKLTEGELSRLRASLVNEGVLAEMAAEMNVGDGLYLG
                     AGELKSGGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFADRVRCADFQNQAKD
                     GKTALQEALQARRFALPKYRIEEQIGHANDSMFVVSCDLGELGFVCRAKGTSRKAAEQ
                     EAAKEALKWLEEKLPLKKKKK"
     gene            1963..2286
                     /locus_tag="ACFM8S_001382"
     CDS             1963..2286
                     /locus_tag="ACFM8S_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HFC6314449.1"
                     /translation="MNAFAQALSSALDRCIATNTVAKQNRPVGFLYREAPVFENDSGW
                     RFFSGDETDEYTDDPDNFSIVSLADIAKTNPETAALLSQPEGSAWELAEDGTFQTVAD
                     WQPQD"
     gene            2334..2810
                     /gene="ribH"
                     /locus_tag="ACFM8S_001383"
     CDS             2334..2810
                     /gene="ribH"
                     /locus_tag="ACFM8S_001383"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFC6314450.1"
                     /translation="MNTIAPNLDGKHLRIGIVQARFTNEIGSEMLKVCCRTLQELGVA
                     DENITVATVPGALEIPIALMNLASSEKFDALIAIGVVIRGETYHFELVSNESGAGVSR
                     VALDYNIPIANAVLTTENDAQAIERIEEKASDAAKVAVECANLVNLLLEEQFEDEE"
     gene            2888..3313
                     /gene="nusB"
                     /locus_tag="ACFM8S_001384"
     CDS             2888..3313
                     /gene="nusB"
                     /locus_tag="ACFM8S_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFC6314451.1"
                     /translation="MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMPDFAKADE
                     ELFNKLFFGTQTNAADYIQKIRPLLDRDEKDLNPIERAVLLTACHELSAMPETPYPVI
                     INEAIEVTKTFGGTDGHKFVNGILDKLATQIRPDEPKRR"
     gene            complement(3680..4714)
                     /gene="pyrC"
                     /locus_tag="ACFM8S_001385"
     CDS             complement(3680..4714)
                     /gene="pyrC"
                     /locus_tag="ACFM8S_001385"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFC6314452.1"
                     /translation="MQTLTIIRPDDMHLHLRDGDALKAVAPYTARQMGRAVIMPNLKP
                     PVVSVADALAYKARIMAALPEGSAFEPLMTLYLTDNATPELVREAKAAGIVAFKLYPA
                     GATTNSDSGVTDLFKLIPVLEEMAKQGILFLVHGEVTDPEIDIFDREAAFIGRVMKPV
                     LAQVPNLKVVFEHITTAEAARLVLEAGDNVAATVTPQHLLLNRNDLLVGGVRPHHFCL
                     PVLKRETHRQALVAAVTGEKAHKFFLGTDSAPHAKSAKENACGCAGMFSAMTAIELYA
                     EVFEKAGALDKLEAFTSKNGARFYGIPENAGTITLVKQSQTVPASVPYGDGELVPMRA
                     GGEIGWTVQY"
     gene            complement(4930..5076)
                     /locus_tag="ACFM8S_001386"
     CDS             complement(4930..5076)
                     /locus_tag="ACFM8S_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314453.1"
                     /translation="MPSEMRMSRPTALFLNIVCAACRFKNKPSGVQCRHFGRIAADLF
                     RNGC"
     gene            complement(5077..5301)
                     /locus_tag="ACFM8S_001387"
     CDS             complement(5077..5301)
                     /locus_tag="ACFM8S_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HFC6314454.1"
                     /translation="MNSETLDVTGLKCPLPILRAKKALAQMQQGDVLTVLATDGGAPG
                     DFEAFCRQTGHVLLDASEQDGVFTLVVQHK"
     gene            5410..6021
                     /locus_tag="ACFM8S_001388"
     CDS             5410..6021
                     /locus_tag="ACFM8S_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HFC6314455.1"
                     /translation="MRRTTLLLLALAAGTSLAAGFSQKDTPINTRYRETPEEAAAREF
                     KEHTAKLPPLPDTHSDGWFDIYVDENYGKQPKILLDSLQIMPAPDGSIRYILNIRSDK
                     GYDNLTAEGIFCARFSIRFGNGKPSSYKVFGYGDTVNRRWIQPRNAEWKPIGGTLSRN
                     DALRTVLYQAFCEGGVPADTKGLVQRLKERAGRYAPSIKPHDK"
     gene            complement(6081..6953)
                     /gene="accD"
                     /locus_tag="ACFM8S_001389"
     CDS             complement(6081..6953)
                     /gene="accD"
                     /locus_tag="ACFM8S_001389"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFC6314456.1"
                     /translation="MSWLDKILPPKIKNRSKDGSSNVPEGLWHKCPSCSATVYSTELQ
                     QNNQVCPKCNHHNPLSARQRLNLLLDEDGREEVAGNVKPIDPLKFKDSKKYPDRLGAA
                     RKLTGEDDALVVMKGMMNGLPVVVAAFEFRFIGGSMGSVVGERFVQGIRRAVADNCPF
                     VCVAASGGARMQEGVNSLMQMTKTSAALHLLTEKRLPFISVLTDPTMGGVSASFAFLG
                     DVVLAEPNALIGFAGPRVIEQTVRETLPEGFQRAEFLLEKGAIDQIVDRRDMKRRISD
                     LITLLCRQDKVSAA"
     gene            complement(6990..7775)
                     /gene="trpA"
                     /locus_tag="ACFM8S_001390"
     CDS             complement(6990..7775)
                     /gene="trpA"
                     /locus_tag="ACFM8S_001390"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFC6314457.1"
                     /translation="MSRIRQAFAALDGGKALIPYITVGDPDIRTTLALMHGMVANGAD
                     ILELGVPFSDPMADGPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLN
                     PVHKMGYREFAQEAAKAGVDGVLTVDSPVETIDPLYRELKDNGVDCIFLIAPPTTEDR
                     IKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKIEYLHQYIDIPIGVGFGISNAESA
                     RKIGRVADAVIVGSRIVKEIENNAGNEAAVVGALVKELKDAVR"
BASE COUNT         1905 a         2406 c         1892 g         1731 t
ORIGIN      
        1 tgcacagata atatcaaccc gtttttaaca aagatattcc cgattgaaac aaaatgccgt
       61 ctgaaacctg ttcggacggc atttgcgtaa agttcagcaa gaaaaactac aaacccagct
      121 cgcgcaggaa gcggatgtcg tccgcccaac cggatttgac tttgacccag accttcaaaa
      181 atactttggt atcaaacagt ttttccatat ccaaccgcgc ttcggtggaa attttcttca
      241 aacgctcccc gcctttgccg attaaaatcg ccttttggct ttccttatcg accaaaacgg
      301 cgatatagat gcggttcaaa ccgtcttcct cttcaaactg ctccacttcg acgttcatcg
      361 cataaggcaa ttcctcgccc aaatagcgga acaatttttc acgcacgatt tccatcgcta
      421 aaaaacgcgc cgatttgtcc gtaaccatat cttcgggata catcggcacg ctttcgggca
      481 gatacggctt gagcagctcc aacaggttgg caatccgcaa tccgtgtttc gcactgaccg
      541 cctccgccgc cgcaaattca aattcggcgc gcacttgggc aacaaacgcc tccagcgcgt
      601 aacggtcttt cgccttgtcc ttgtcgattt tgttgaccac taaaatgacc ggcgtgtgct
      661 tgggcagttg tttcaacacg acacggtcgg catcggtaaa gcgcatcgct tccacgacga
      721 aaaccaccac atccacgccg ccaagcgcct cggtaacgtt ttgattcagc ctgtcgttga
      781 gcgcgttgcg gtggtcggtt tgaaagccgg gcgtatcgac aaacacgaac tgcgcggtat
      841 cgtcggtata aatccccgtt acgcggttgc gcgtcgtctg cgcctttttg ctggtgatgc
      901 tgattttctg accgatgaga tggttcatca gcgttgattt gcccacgttc ggacgaccga
      961 caatcgccac gaagccgcaa cggtatccgc cggcggcgcg ttcccctgca aggaaggttt
     1021 caatatccat attcgctttc atatccacat ttcagacggc atattcacgc gccgcctcat
     1081 ttctttttct tcttcagcgg cagcttctct tccagccatt tcaaagcctc tttcgccgcc
     1141 tcctgttccg ccgccttgcg gctcgtccct ttggcacggc acacgaaacc cagttcgccc
     1201 aaatcgcagg aaaccacaaa catactgtcg ttggcatggc cgatttgctc ttcgatacgg
     1261 tatttcggca aagcaaagcg gcgtgcctgc aacgcctcct gcaaagcagt tttgccgtct
     1321 tttgcctgat tttggaaatc ggcacaccgg acgcggtcgg caaacaaatg gcgcaccacc
     1381 ttttccgccg tgttgaaatc ggcatcgaag ctgaccgccg caaacatcgc ctccatcgcg
     1441 tccgccagta tcgaaggccg tctgaagccg ccgctcttca actcgcccgc ccccaagtac
     1501 agaccgtcgc cgacattcat ttccgccgcc atttccgcca gcacgccctc attgaccaga
     1561 cttgcccgca accgcgacaa ctcgccctcg gtcaacttcg gaaacgtgtc aaacagcatc
     1621 cgcgccaccg tataattcaa aatcgaatca ccgacaaact cgaaccgctc gttgtgcttc
     1681 gcatgatggc tcctgtgcgt caaagcctgc cgcaaaagcg aaatatcgcg gaacgcgtag
     1741 cccagtctct tctgtatcgc cgcgtgtgcc tgctgtttca aaacatcgtc tttcattacc
     1801 gtcttccgca aaaaacagcc cgaatgcgtt tccatactcc aaaaatacag aaacacctta
     1861 aggctgcaaa cggcgcacat tgtaccacca aaccgccgcc gtctgccgac aacgcacgaa
     1921 tcgtggtaaa atccgtaagt tttcccccaa cgtattcccg taatgaacgc atttgcacaa
     1981 gcactttcct cggcactcga ccgctgtatc gccaccaaca ccgtcgccaa acaaaaccgg
     2041 cccgtcggct tcctctaccg cgaagccccc gtttttgaaa acgacagcgg ctggcgtttc
     2101 ttcagcggcg acgagaccga cgaatacacc gacgatcccg acaacttcag catcgtcagc
     2161 ctcgccgaca tcgccaaaac caaccctgaa accgccgcac tgctttccca acccgaaggc
     2221 agcgcatggg agcttgccga agacggcaca ttccaaaccg tcgccgactg gcagccgcaa
     2281 gactgaaacc ttcagacggc attccgaaac ctccgaatac caaaggacac ccaatgaaca
     2341 ccatcgcccc caacctcgac ggcaaacacc tccgcatcgg catcgtacag gcacgtttca
     2401 ccaacgaaat cggcagcgaa atgctcaaag tctgctgccg caccctccaa gaattgggcg
     2461 tggcagacga aaacattacc gtcgccaccg tacccggcgc acttgaaatc cccatcgcac
     2521 tgatgaacct tgcctcttcc gaaaagtttg acgcactgat tgccatcggt gtcgtcatcc
     2581 gtggcgaaac ctaccatttc gagctggttt ccaacgaatc cggagcaggg gttagccgcg
     2641 tcgcactcga ctacaacatc ccgattgcca atgccgtcct aaccaccgaa aacgacgcgc
     2701 aggcaatcga acggattgaa gaaaaagcct cggatgccgc caaagtcgcc gtcgaatgcg
     2761 ccaacctcgt caacctgctg ctcgaagaac agtttgaaga cgaagaataa caaacagggc
     2821 gtacaccaat acgccctttc cgtttcagac ggcataccgt gccgcccaac cgtttcaagg
     2881 aaaaatcatg aaaacagccc gccgccgttc ccgcgagctt gccgtacaag ccgtttacca
     2941 atcccttatc aaccgcaccg ccgcgcccga aattgctaaa aacatccgcg aaatgcccga
     3001 ctttgccaaa gcggacgaag aattgttcaa caaacttttc ttcggtacgc aaaccaacgc
     3061 agcggactac atccaaaaaa tccgccccct gctcgaccgc gacgaaaaag acctcaatcc
     3121 catcgaacgc gccgtcctgc tgaccgcctg ccacgagctg tccgccatgc ccgaaacacc
     3181 ctaccccgtc atcatcaacg aagccatcga agttaccaaa accttcggcg gcacggacgg
     3241 gcacaaattc gtcaacggca tcctcgacaa actcgccacc caaatccgcc ccgacgagcc
     3301 caaacgccgt tgatgcatca caaatttaaa gaaagttcta tgtccttatt tctgctttct
     3361 ttgtttcgtc attcccacgc aggtgggaat ttggtttttt gaattttggg tatttcaaat
     3421 aaattctgaa acttcaatct cgtcattccc gcgcaggcgg gaatccggtt ttttgagttg
     3481 ctgccgtttt tgataaactt tggtcgcatc gaatttctgg attcctgcct tcgtgggaat
     3541 ggcgaaattt gaaaattgtg ggaatttatc ggaaataacc gaaacgtaac aaactcaaac
     3601 gccgttgacg tatcaatcag tcagcaacac aaatgccgtc tgaaaaacct ttcagacggc
     3661 attttgcttg accgttcaat caatactgta ccgtccagcc gatttcgccg cccgcgcgca
     3721 tcgggacaag ttcgccgtcg ccgtagggaa cacttgcggg gacggtttgg ctttgtttga
     3781 cgagggtgat cgtgccggcg ttttccggaa tgccgtaaaa ccttgcgccg tttttcgagg
     3841 taaaggcttc gagtttgtcc aacgcgcctg ctttttcaaa cacttcggcg taaagctcga
     3901 tggcggtcat cgcgctgaac ataccggcgc aaccgcaggc gttttcttta gcggatttgg
     3961 cgtgcggcgc ggaatctgtg ccgaggaaga atttgtgcgc cttctcgccg gtaacggcgg
     4021 caaccaatgc ctgacggtgg gtttcgcgtt tgagtacggg caggcagaaa tgatgggggc
     4081 gcacgccgcc gaccaagagg tcgttgcggt tgagcaggag gtgttgcggg gtaacggtgg
     4141 cggctacgtt gtcgcctgct tccaaaacca ggcgggcggc ttcggcggtg gtgatgtgtt
     4201 cgaacacgac tttaagattc ggcacttgcg ccaaaacggg tttcatcacg cgcccgataa
     4261 aggcggcttc gcggtcgaag atgtcgattt cggggtcggt tacttcgccg tgaacgagga
     4321 acaaaatgcc ctgtttcgcc atttcttcca acacggggat gagcttgaac aggtcggtta
     4381 cgccggaatc ggaattggtg gtcgcgccgg cagggtagag tttgaaggcg acgatgccgg
     4441 cggctttggc ttcgcgtaca agttcgggcg tggcgttatc agtcaaataa agcgtcatca
     4501 acggctcaaa cgcgctacct tcgggcaacg ccgccataat gcgcgctttg taggcaagcg
     4561 cgtcggctac actgacgaca ggcggtttga ggttgggcat aatgacggcg cgccccattt
     4621 ggcgggcggt ataaggggca acggctttga gcgcgtcgcc gtcgcgcagg tgcaggtgca
     4681 tatcgtcggg gcggataatg gtcagggttt gcatggttgt tcctttttag gttttggctg
     4741 cgtttgaata cccggtcgat tccatccgtt tgccggggga aagccggtta ttccgtccgg
     4801 tcggaaaccg gttccattgg atgaaaggca ttttgtccga ctgattgggt tgcatcaacg
     4861 gaatcttcaa acacagcctt tagattttga ttgtgcttgg gttttgcttc tttgccgacg
     4921 gcaaggcggt taacagccat tccggaacaa atcggcggcg atacgcccga aatgccgaca
     4981 ctgcacgccg gacggtttgt ttttgaaacg gcacgcggca catacgatat tcaaaaacaa
     5041 cgccgtcggg cgggacatcc gcatttcaga cggcatttat ttgtgttgga cgaccagcgt
     5101 gaacacgccg tcctgttcgg aagcatccaa cagcacatga ccggtttggc ggcaaaaagc
     5161 ctcaaaatcc cccggtgcgc cgccgtcggt tgccagaacg gtcaacacgt cgccctgctg
     5221 catttgcgcc aaagcctttt tggcacgcaa aataggcaaa gggcatttca gcccggttac
     5281 gtctaaagtt tcgctgttca tcgtttgtac cgtttaaaaa atgggaatcg gggggagatt
     5341 ataccccagt tgcttgcagc cccctttgcc aacccataaa ataacgccgt tttcaatttg
     5401 gagaaactta tgcgccgcac caccctcctg ctgctcgcac tcgccgctgg cacgtccctt
     5461 gctgccggct tcagccaaaa agacacgccg atcaacaccc gctaccgcga aacgcccgaa
     5521 gaagccgccg cccgcgaatt taaagaacat accgccaaac tgccgccgct gcccgatacg
     5581 cactcggacg gctggttcga catttatgtg gatgaaaatt acggcaaaca gccgaaaatc
     5641 ctgctcgaca gcctgcaaat tatgcccgcg cccgacggca gcatccgcta cattctcaat
     5701 atccgctccg acaaaggcta cgacaacctg actgccgaag gcatattctg cgcccgcttc
     5761 tccatcaggt tcggcaacgg caaaccttcc tcctataaag tgttcggcta cggcgacacg
     5821 gtcaaccgcc gctggataca gccccgaaac gccgaatgga aaccgatagg cggtacatta
     5881 agccgcaacg acgcactgcg caccgtcctg tatcaggcat tctgcgaggg cggtgtaccc
     5941 gccgacacga aaggtttggt acaacgcctg aaagaacgtg ccggacgcta tgccccgtcg
     6001 ataaagccgc acgacaaata accagacaca aaaataccgt ctgaaacatc ggtttcagac
     6061 ggtattcgat tcatcagcca tcaggcggca gaaactttgt cctgacggca caacagcgta
     6121 atcaaatcac tgatgcgccg cttcatatcg cggcggtcga caatctggtc gattgcgcct
     6181 ttttccagca ggaactcggc gcgttggaag ccttccggca gcgtttcgcg caccgtctgc
     6241 tcaatcacgc gcggaccggc aaaaccgatc agcgcgttcg gttcggcaag cacgacatcg
     6301 cccaaaaatg cgaagctggc ggatacgccg cccatagtcg gatcggtcaa caccgatata
     6361 aatggcaggc gtttttccgt cagcaaatgc agcgcggcac tggttttcgt catctgcatc
     6421 aatgagttca caccctcctg catacgcgca ccgccggaag ccgccacaca gacgaacgga
     6481 caattgtcgg caaccgcccg acggatacct tgtacgaatc gttcgcccac aaccgaaccc
     6541 atcgaaccgc cgataaagcg gaattcaaac gcagcaacga cgacgggcag gccgttcatc
     6601 atccccttca tcaccaccag cgcgtcatct tccccggtca gcttgcgtgc cgcccccaaa
     6661 cggtcgggat attttttact gtctttaaac ttcaaaggat ctatcggttt gacatttccg
     6721 gcaacttcct ccctgccatc ctcatccaaa agcaggttca ggcgttgtcg tgccgacaac
     6781 ggattgtggt ggttgcattt cggacaaacc tgattgttct gctgcaactc ggtcgaataa
     6841 acggttgccg aacaagacgg acatttgtgc cacaaaccct cgggaacatt ggaagaaccg
     6901 tctttgctgc ggtttttgat tttgggtggc aggattttat ctaaccagct catggacaac
     6961 tcctaaaata tttagaatgc gtgacagaat cagcgcacgg catccttcaa ctctttgacc
     7021 aaagcaccga cgaccgcagc ctcgttgccc gcattgtttt caatctcttt cacaatccgg
     7081 ctgccgacaa tgaccgcgtc ggcaacccgg ccgattttgc gcgcgctttc cgcattgctg
     7141 atgccgaaac cgacaccgat gggaatatcg atatactgat gcaaatactc tattttacgc
     7201 gaaacctcat ccgtatccaa acttgccgcg cccgttacgc ccttgagcga aacataatag
     7261 acaaatccgc ctgccagctc ggcaatggtt ttaatacggt cttccgtcgt cggcggcgca
     7321 atcaggaaaa tacagtcgac cccgttatcc ttcagctcgc gatagagcgg atcgatggtt
     7381 tcgacagggg aatccaccgt caacacgccg tccacacccg cctttgcggc ttcctgagca
     7441 aactcccgat aacccatctt atgtacaggg ttcaaatatc ccatcaaaac aaccggcgtt
     7501 tgcgtgtcgg tttcacggaa ttttctgacg acatccaaga catcgcgcag cgaaatcccg
     7561 tttgccaacg cccgctccgc cgcacgctga ataaccggcc catccgccat cggatcggaa
     7621 aacggcacac ccaactccaa aatatccgca ccgtttgcaa ccatgccgtg catcaatgcc
     7681 aaagttgtcc gaatatcggg gtcgcccacc gtaatatagg gaatcaatgc ctttccgcca
     7741 tccaaagcgg caaaagcctg ccggattctg ctcatttgtc tcttccatcc tatcggcaaa
     7801 tatgccgtaa aacacaatca gtaaattttt cccagtataa catttgcggt acggataggc
     7861 gaatacggca aacagaaacc gcgcaaatca gagccgccgt ttgaacgagg cggaaataaa
     7921 cggcaagcat ctcg
//
LOCUS       DAXHSF010000093         7926 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.093, whole genome shotgun sequence.
ACCESSION   DAXHSF010000093 DAXHSF010000000
VERSION     DAXHSF010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7926
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.093"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(49..2091)
                     /gene="recG"
                     /locus_tag="ACFM8S_001391"
     CDS             complement(49..2091)
                     /gene="recG"
                     /locus_tag="ACFM8S_001391"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFC6314458.1"
                     /translation="MMSPETQKQLKITDVSAKKLDKLNLHTAWDLVLHLPLRYEDETH
                     IMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLIVQIADGSGSVLFLRFIHFYASHQKQ
                     TAVGKRIRAVGEIKHGFYGDEMIHPKIRDAEGGGLAESLTPVYPTVNGLNQPTLRRII
                     QTALDVTPLHDTLPDALLCRLKLPHLAESLRLLHSPPPSFTIHQLSDGTFPAWQRLKF
                     DELLAQQLSMRLARQKRIGGTAAALRGDGTLTQALHHALPFALTDAQEKAVSEICHDM
                     AQTHPMHRLLQGDVGSGKTIVAALSALTAIESGAQVAVMAPTEILAEQHFIKFKQWLE
                     PLGIEVVRLFGSLRKKAKDEAKAKLADGTVKIAVGTHALFSDGVEFQNLGLVIVDEQH
                     RFGVAQRLALKNKGRDVHQLMMSATPIPRTLAMSFFADLDVSVIDELPPGRTPIKTRL
                     VNNVRRAEVESFVLNICRKGQQAYWVCPLIEESETLQLQTAAETLARLQTALPELNIG
                     LVHGRMKAAEKAEVMARFSSGELNVLVATTVIEVGVDVPNAALMVIEHAERMGLAQLH
                     QLRGRVGRGAAESVCVLLFAEPLGELAKARLKVIYEHTDGFEIARQDLNIRGPGEFLG
                     ARQSGVPMLRFAKLEEDLHLLEQAREIAPILIEQNPEIVEAHLARWLSGREGYLGV"
     gene            complement(2177..2620)
                     /gene="secB"
                     /locus_tag="ACFM8S_001392"
     CDS             complement(2177..2620)
                     /gene="secB"
                     /locus_tag="ACFM8S_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFC6314459.1"
                     /translation="MSEELQPVFSIERLYVKDLSLEVPHAPQIFLEQGEPEVDMRVST
                     GSQKLEDGYYNVDVTVTVTAKLDNERTMFLNEVTQSGIFRLENIPEEDVQLLLGVACP
                     NILFPYAREAVSGTVTRAGFPPVLLAPINFEAIYQQQQEAEAAGA"
     gene            complement(2641..2898)
                     /gene="grxC"
                     /locus_tag="ACFM8S_001393"
     CDS             complement(2641..2898)
                     /gene="grxC"
                     /locus_tag="ACFM8S_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFC6314460.1"
                     /translation="MQTVTMYTGPFCPYCAMAKRLLHTAGVGHIDEIRVDASPEAFAE
                     MQQLSGQRSVPQIFIGETHVGGFTDLYRLQQEGGLDGLLNP"
     gene            3112..4605
                     /gene="rng"
                     /locus_tag="ACFM8S_001394"
     CDS             3112..4605
                     /gene="rng"
                     /locus_tag="ACFM8S_001394"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFC6314461.1"
                     /translation="MLSGLPIPKDIARPPETILVNITPQETRVAVLEENNICELHIER
                     NSEHSLVGNIYLGVVRRVLPGMQSAFIDIGLERAAFLHIVDVLEQRRNPEETQRIEHM
                     LFEGQSVLVQVIKDPINTKGARLSTQISLAGRFLVHLPQEDHIGVSQRIEDDAERSSL
                     RERLDKLLPENACRGYIIRTNAENATDEQLQSDIDYLTKVWEHIQEQAKIRPPETLLY
                     QDLPLSLRVLRDMVGCDTQKILVDSTVNHGRMTRFAEQYVHGALGRIELFKGERPLFE
                     THNVEQEISRALQPRVNLNFGSYLIIESTEAMTTIDVNTGGFVGARNFDETIFRTNLE
                     ACHTIARELRLRNLGGIIIIDFIDMAQESHREAVLQELAKALAFDRTRVTLHGFTSLG
                     LVELTRKRSRENLNQVLCEPCPSCQGRGRLKTPQTVCYEIQREIVREARRYDAESFRI
                     LAAPNVIDLFLDEESQSLAMLIDFIGKPISLAVETAYTQEQYDIVLM"
     gene            complement(4640..5890)
                     /locus_tag="ACFM8S_001395"
     CDS             complement(4640..5890)
                     /locus_tag="ACFM8S_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC6314462.1"
                     /translation="MFVPLAMLAGMFSYYETFHETEALQDDLLRQAALYVAPDSKPET
                     LPEGDGDTRILVQMPQQEDPVVSLPAHLADGLHTLQADGDDDYYRVYIRTTERGRIAV
                     MQESEYREDLAADAARQSVLPLLAALPLMILLTVWITHKAMRPVRKLSQSLEQRRIND
                     LSALSVDNIPSEIRGFVTAINLLLKRADEDIRHRQRFVADAAHELRTPMTALSLQAER
                     LNNMSLPPDAARQSAVLQQSIRRNKHLLEQLLALARSQSDETPLAKTTFGLQSRFRQV
                     LQELMPLALEKRQDIGVAVGGDVEIFADETEIYTLVKTFVDNAIRYTPSEGRIDLGFT
                     DEGKYLAVWVEDNGNGIPESERVRVLDPFYRILGTEQQGTGLGLSIADTLAKKYGGYL
                     ELTDSRRFGHGLLIRALLDKETLK"
     gene            complement(5903..6060)
                     /locus_tag="ACFM8S_001396"
                     /pseudo
     CDS             complement(5903..6060)
                     /locus_tag="ACFM8S_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003824008.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(6283..7596)
                     /locus_tag="ACFM8S_001397"
     CDS             complement(6283..7596)
                     /locus_tag="ACFM8S_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HFC6314463.1"
                     /translation="MLTFIGLLIIGVIVWLLLTEKVSPIIALILVPLFGALLAGFDVS
                     QLKEFYSGGTKSVMQIVIMFMFSILFFGIMNDVGLFRPMIGGLIKLTRGNIVAVSVGT
                     VLVSVVAQLDGAGATTFLLVVPALLPLYKRLHMNPYLLFLLLTSSAGLINLLPWGGPT
                     GRVASVLGADVGELYKPLLTVQIIGVVFILALSLLLGVREKRRIVRELGALPAVADLI
                     KPVPLSEEEQKLARPKLFWWNVLLFLAAMSLLFSGIFPPGYVFMLAATAALLLNYRSP
                     QEQMERIYAHAGGAVMMASIILAAGTFLGILKGAGMLDAISKDIVHILPDALLPYLHI
                     AIGVLGIPLELVLSTDAYYFGLFPIVEQITSQAGVAPEAAGYAMLIGSIVGTFVTPLS
                     PALWMGLGLAKLSMGKHIRYSFFWAWGLSLAILASSIAAGIVPLP"
BASE COUNT         1874 a         2329 c         2056 g         1667 t
ORIGIN      
        1 tcagatttcc gttacacttt ttttctaata tttcagacgg cattttgctc acacgcccaa
       61 ataaccttcc ctgccggaaa gccatcttgc caaatgcgct tcgacgattt cggggttttg
      121 ctcaatcagt atcggggcga tttcgcgcgc ttgttccaaa aggtgtaagt cttcttcgag
      181 cttggcgaaa cgcagcatag gcacgccgct ttgacgcgcg ccgagaaatt cgccggggcc
      241 gcggatgttt aggtcttggc gggcgatttc aaagccgtcg gtgtgttcgt agatgacttt
      301 cagccgcgct ttggcgagtt cgcccaaggg ttcggcaaac aggaggacgc acacgctttc
      361 tgccgcgccg cgcccgaccc gaccgcgtaa ttggtgcagc tgcgccaagc ccatgcgctc
      421 ggcgtgttcg atgaccatca gggcggcatt gggcacatct acgccgactt cgatgacggt
      481 ggtggcgacc aagacgttca gctcccccga agaaaaccgt gccatcactt cggccttttc
      541 ggcggccttc atgcgcccgt gtaccagtcc gatattcagc tcgggcaatg ccgtctgaag
      601 ccgggcgagg gtttcggcgg cggtttgcaa ttgcagggtt tcgctttctt caatcagcgg
      661 acaaacccag tatgcctgct gccctttgcg gcagatgttg aggacgaagc tttcgacttc
      721 ggcgcggcgg acgttgttga cgaggcgggt tttaatcggg gtgcggccgg gcggcaattc
      781 gtcgatgacg gacacgtcca aatcggcaaa aaaactcatg gcgagcgtgc gcgggatggg
      841 cgtggcggac atcatcaatt gatggacgtc gcgccctttg tttttgaggg caaggcgttg
      901 agcgacaccg aagcggtgtt gttcgtccac aatcaccaag cccaaatttt ggaactcgac
      961 gccgtctgaa aacagggcat gcgtgccgac ggcgattttg acggtgccgt cagccagttt
     1021 ggctttggct tcgtctttgg cttttttacg caaactgcca aaaaggcgga caacttcaat
     1081 gcccaaaggt tcgagccatt gtttaaattt aataaaatgt tgttcggcaa ggatttcggt
     1141 cggcgccatt acagccacct gcgcaccgga ttcgatagcc gtcaaagcag acaaagcagc
     1201 cacaatggtt ttgccgctgc cgacatcgcc ttgcagcagg cggtgcatgg ggtgggtttg
     1261 cgccatatcg tggcagattt cggacacggc tttttcttgt gcatcagtca gggcaaacgg
     1321 tagggcgtgg tgcagggctt gggtcaacgt gccgtcgccg cgcaatgccg ccgccgtgcc
     1381 gccgatacgc ttctgccgcg ccaagcgcat cgaaagctgt tgcgccaaaa gttcgtcgaa
     1441 tttgagccgt tgccatgcag gaaacgtgcc gtctgaaagt tgatgaatgg tgaaactcgg
     1501 cggcggcgaa tgcaaaaggc gcaggctttc ggcaaggtgc ggcagcttca gacggcacag
     1561 cagggcatcg ggcagcgtgt cgtgcaacgg cgtaacgtcc aacgccgtct gaatgatgcg
     1621 gcgcaaagtg ggctggttca aaccgtttac ggtcgggtaa accggcgtga ggctttccgc
     1681 caaaccgccg ccctcggcat cgcggatttt gggatgaatc atctcgtcgc cgtaaaagcc
     1741 gtgtttgatt tcgcccacgg cgcggatgcg tttgccgacc gccgtctgtt tctgatggct
     1801 ggcgtaaaag tggatgaagc gcagaaaaag gacgctgccg gaaccgtcgg cgatttggac
     1861 aatcaactgc ttgcgcggtt tgaacgttac ttcctgatgg ataacctcgc cctcgacctg
     1921 gcacggcacg ccaatcggcg cgtccttaat cggcataatg tgcgtctcgt cctcgtaacg
     1981 cagcggcagg tgcaacacca aatcccacgc ggtatggagg ttgagtttgt cgagcttctt
     2041 ggcggaaaca tcggtgattt tgagctgttt ctgggtttcg ggcgacatca taggcagatt
     2101 cctttgaacg cacctatttt atccgaaaac aaaaataccg tctgaaacgg attcagacgg
     2161 catcggcagg caggaatcaa gccccggcgg cttcggcttc ctgctgttgt tggtaaatcg
     2221 cctcaaaatt aatcggcgcg agcaggacgg gcgggaagcc ggcacgtgtt accgtaccgg
     2281 aaacggcttc gcgcgcgtaa gggaagagga tgttcggaca cgccacgccc agcagcagct
     2341 gcacatcttc ttcggggatg ttttccaaac ggaagatgcc actttgggtt acttcgttca
     2401 aaaacatcgt gcgctcgtta tccagcttgg cggtaacggt tacggtaacg tccacgttgt
     2461 agtagccgtc ttccagcttt tggctgccgg tggaaacgcg catatccact tcaggctcgc
     2521 cctgttccaa aaagatttgc ggcgcgtgcg gcacttccaa agacaagtct ttgacataca
     2581 gtcgctcgat gctgaatacg ggttgcagtt cttcgctcat tttgttttcc tagttggggg
     2641 ttaagggttc agcagtccgt ccagcccgcc ttcctgctgg aggcggtaga ggtcggtaaa
     2701 tccgccgacg tgcgtttcgc cgatgaaaat ctgcggcacg ctgcgctgtc ccgaaagctg
     2761 ctgcatttcg gcaaaggctt cggggcttgc atcgacacgg atttcgtcga tatgtccgac
     2821 acctgccgtg tgcagcagcc ttttcgccat cgcgcagtag ggacaaaacg gacctgtgta
     2881 catggtaacg gtctgcatat tgggtttccg aaagttttgc aatgataatc aatatagggg
     2941 catttcccct gtttggcaag tgcggaacag atgcacgttc aaacggcatg tgcggaatgt
     3001 gtcaaagttt cttttttaaa gtatgatagg cattgtgaaa aatatttttg cacccgcgct
     3061 gcgcggcgga acgggtgcaa aaatattttt attacatttt caggaaaaac catgttgtca
     3121 ggactcccca tccccaaaga catcgcgcgc ccgcccgaaa cgatattggt caacatcacg
     3181 ccgcaggaaa cgcgcgtagc ggtgttggag gaaaacaata tctgcgagct gcacatcgag
     3241 cgcaacagcg aacacagcct ggtcggcaat atctatctgg gcgtggtgcg ccgcgtgctg
     3301 cccgggatgc agagcgcgtt tatcgacatc ggcttggaac gcgcggcgtt tttacacatc
     3361 gtcgatgtcc tcgaacaacg ccgcaacccc gaagaaaccc agcgcatcga acatatgctg
     3421 tttgaagggc agtctgtttt ggtgcaggtc atcaaagacc cgatcaacac caaaggcgcg
     3481 cggctttcca cccaaatctc gctggcgggg cgtttcctcg tccatcttcc gcaagaagac
     3541 cacatcggcg tgtcccaacg catcgaagac gatgccgaac gcagcagcct gcgcgaacgc
     3601 ctcgacaagc tcctgccgga aaatgcctgc cggggctaca tcatccgcac caacgccgaa
     3661 aacgccaccg acgaacagct ccagtccgac atcgactacc tgaccaaagt gtgggaacac
     3721 atccaagaac aggcgaaaat ccggccgccg gaaaccctgc tttatcagga tttgcccttg
     3781 agtttgcgcg tattgcgcga tatggtcggc tgcgacacgc aaaaaatcct cgtcgattcc
     3841 accgtaaacc acgggcgcat gacgcgtttt gccgaacaat acgtccacgg cgcattgggc
     3901 aggatagaat tgttcaaagg cgaacgcccg ctgtttgaaa cccacaacgt cgaacaggaa
     3961 atcagccgcg ccctgcaacc gcgcgtcaac ctcaacttcg gcagctacct gattatcgaa
     4021 tccaccgaag ccatgaccac gatagacgtg aacaccggcg gcttcgtcgg cgcacgcaac
     4081 ttcgacgaaa ccatcttccg caccaacctc gaagcctgcc acaccatcgc ccgcgaattg
     4141 aggctacgca acctcggcgg catcatcatc atcgacttca tcgatatggc gcaggaaagc
     4201 caccgcgaag ccgtgttgca ggagcttgcc aaagccctcg ccttcgaccg tacccgcgtt
     4261 accctgcacg gttttaccag cctggggctg gtcgagctga cgcgcaaacg ctcgcgcgaa
     4321 aacttaaacc aagtcctctg cgaaccctgc ccttcctgcc aaggcagagg ccgtctgaaa
     4381 acgccgcaaa ccgtgtgcta cgaaatccag cgcgaaatcg tccgcgaagc gcgccgttac
     4441 gatgccgaaa gtttccgcat cctcgccgcc cccaacgtca tcgatttgtt tttggacgaa
     4501 gaatcgcaat ccttggcaat gctgatagat ttcatcggca aaccgatttc tctggcggtc
     4561 gaaaccgctt acacgcagga acaatacgat attgtgctga tgtaaaaaat gccgtctgaa
     4621 gccttcagac ggcatctgtc tatttcaggg tttccttgtc caacaaggcg cgtatcagca
     4681 gaccgtgtcc gaacctgcgg ctgtcggtca gttccaaata tccgccgtat tttttggcta
     4741 gcgtgtcggc aatcgacagc cccagccccg tcccctgctg ctccgttccc aaaatacggt
     4801 aaaacggatc gaggacgcgg acgcgttcgg attccggaat gccgttcccg ttatcttcca
     4861 cccacacggc aagatatttc ccttcgtctg tgaaacccaa atctatcctg ccttcggacg
     4921 gggtataacg tattgcgtta tcaacaaacg ttttaaccaa ggtatagatt tcagtttcat
     4981 cggcaaatat ttcgacatca cctccgaccg ccacaccaat gtcctgacgt ttttccaagg
     5041 caagcggcat cagttcctgc aacacttggc ggaaacggct ttgcagaccg aatgtcgttt
     5101 tcgccaaagg ggtttcgtcc gactgcgaac gcgccagtgc caaaagctgc tcgagcaggt
     5161 gtttgttgcg ccgtatgctt tgctgcaaaa cggcagactg ccgcgccgca tcgggtggca
     5221 gagacatatt gttgagccgt tccgcctgaa gggaaagggc tgtcatcggc gtacgcaatt
     5281 cgtgtgccgc gtcggcgaca aaccgctgac ggtggcggat gtcttcatcc gcacgtttca
     5341 aaagcaagtt gatggcggtt acgaaccctc tgatttcact gggaatattg tccacactca
     5401 aggcagacag gtcattgatt cggcgttgtt cgagactttg cgacaatttg cggacggggc
     5461 gcatggcttt gtgcgtaatc cacacggtca gcagtatcat cagcggcagt gccgccagca
     5521 ggggcaacac gctttgccgt gccgcatccg ccgccaaatc ttcccggtat tcgctttcct
     5581 gcatcacggc aatccgcccc cgctccgtcg tgcggatata gacgcggtaa taatcgtcgt
     5641 ccccgtccgc ctgaagcgtg tgcagaccgt ccgccagatg cgcaggcagg ctgacaacag
     5701 ggtcttcctg ctgcggcatc tgtaccaaaa tacgcgtatc gccgtcgccc tcgggcaaag
     5761 tttcgggttt ggaatcgggg gcgacgtaca atgccgcctg acggagcagg tcgtcctgca
     5821 acgcttccgt ttcgtggaag gtttcgtagt aggaaaacat acctgcaagc attgccagcg
     5881 gaacaaacat ccaaaccgtc cgccccggca aacccaaatc cagcagcatc aagtcataag
     5941 gctgggcagc ggcagccgcg cgccgttttt gacccaatcc accgcatagc cgccgtcttt
     6001 caaacttgcc gacaccgcct ccgcaatcat cgcatcgtct tccaccagca aaacacgcat
     6061 caactttccc tttcagacaa accgtgccta ttctaacacc ccaaaattag ccgcaattta
     6121 gcggccttta cgcttgccgg tatttttcaa aactgcagca caaaaaaacc gcgccggcaa
     6181 ctgccttcag acggcattgg ggcgcgattg caacacacgg gcaggcaggc agagcctgcg
     6241 acagaccaca ggaacgattc gggcttcaga cggcttcgcc gtttacggca gaggcacgat
     6301 tcctgccgct atcgaactgg ccaatatcgc cagcgacaaa ccccacgccc aaaaaaacga
     6361 ataacggatg tgtttgccca tcgacaattt cgccaaaccc aagcccatcc acaaagccgg
     6421 cgaaagcggc gtaacaaaag tgccgacgat actgccgatc aacatcgcat aacctgctgc
     6481 ttcgggcgcc acgcccgcct gcgaggtaat ctgctccaca atcggaaaca gtccgaaata
     6541 ataagcgtcc gtactcaaaa ccaactcaag cggaatgccc aacacaccga tggcaatatg
     6601 cagataaggc agcagcgcgt ctggcaggat atgcacaatg tctttggaaa tcgcgtccaa
     6661 catccccgca cccttcaaaa tccccaaaaa cgtacctgcc gccaaaataa tggacgccat
     6721 catcaccgcg ccgccggcgt gggcataaat ccgctccatc tgttcctgcg ggctgcggta
     6781 attcaaaagc aacgccgccg ttgcagccag cataaataca taacccggcg ggaagatgcc
     6841 cgaaaaaagc aggctcatcg ccgccaaaaa cagcaggaca ttccaccaaa acagtttcgg
     6901 acgcgccaat ttttgttctt cttccgacaa aggcaccggc tttatcaaat ccgccacggc
     6961 gggcaacgcg cccaactccc ggacaatccg ccttttttca cgcacaccca aaagcaggga
     7021 cagcgcaagg ataaacacta caccgataat ttgcaccgtc aacaaaggtt tatacaattc
     7081 gcccacatct gcgcccaaca cgcttgcaac ccgcccagtc ggcccgcccc acggcagaag
     7141 gttaatcaat cccgcactgg aagtcagcag caaaaacagc aggtaaggat tcatatgcag
     7201 acgcttgtaa agcggcaaaa gggcggggac gaccaataaa aacgtcgtcg cacccgcccc
     7261 gtccaactgc gccaccaccg acaccaagac cgtccccaca ctcactgcca cgatattacc
     7321 ccgagtcagc ttaatcaaac cgcctatcat cggacggaac agccccacat cgttcatgat
     7381 tccaaaaaac aaaatggaaa acataaacat aatcacaatc tgcatcaccg atttggtgcc
     7441 gcccgaataa aattctttta attgggatac atcaaacccc gccagcaacg ccccaaacag
     7501 cggcaccaag attaatgcga tgatgggcga cactttttcc gtcagcagca accatacgat
     7561 gaccccgata atcagcagtc cgataaacgt cagcatcatt tctcctttat tttattttaa
     7621 acagaaaacc gaccgtgcag gcaaaaccgc ccacagacgc gggataagcc ttgcattcta
     7681 cttttttatt ttgaaacaag tcaatcggtc atttcctccc atttacgcct gccgccattc
     7741 ctgtatccgt ccgtcatttc acggcggcaa ccgatacgga acaaccggca aaccggtatc
     7801 gggacggcgc gggggcattc atcccggtac gccgattcaa acgaaaccgc ccctatcatt
     7861 gcggagcgcg gggcgtgccg tacacgtggg attttatagt ggattaacaa aaatcaggac
     7921 aaggcg
//
LOCUS       DAXHSF010000094         7902 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.094, whole genome shotgun sequence.
ACCESSION   DAXHSF010000094 DAXHSF010000000
VERSION     DAXHSF010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7902
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.094"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(145..1941)
                     /locus_tag="ACFM8S_001398"
     CDS             complement(145..1941)
                     /locus_tag="ACFM8S_001398"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFC6314464.1"
                     /translation="MNTVSNYLSALREAMKAQGLDALVIPSADPHLSEYLPEHWQARR
                     ELSGFTGSVGTFVVTADEAGVWVDSRYWEQAAKQLAGSGIELQKSGQVPPYNEWLAAN
                     LPENAAVGIPSDMVSLTGKRTLAQSLTAKNIRIEHPDDLLDQVWTSRPALPAETVFIH
                     DPAYVSETAAEKLARVRAVMAEKGADYHLVSSLDDIAWLTNLRGSDVPFNPVFVSFLL
                     IGKDNAVLFTDRCRLNAEAAAALQTAGIAVEPYAQVAGKLAQIGGVLLIEPNKTAVST
                     LVRLPKSMRLIEGINPSTLFKSCKSEADIARIREAMEHDGAALCGFFAEFEDIIGNGG
                     SLTEIDVDTMLYRHRSVRPGFISLSFDTIAGFNANGALPHYSATPESHSIISGNGLLL
                     IDSGAQYKGGTTDITRVVPVGTPTAEQKRDNTLVLKAHIALAEAVFPENIPSPLIDAI
                     CRKPLWQAQCDYGHGTGHGVGYFLNVHEGPQRIAFAAPATPETAMKKGMVTSIEPGLY
                     RPGKWGIRIENLAANQAVADPQETEFGSFLCFETLTLCPIDTRLMDTALMTDGEIDWV
                     NRYHAEVRRRLEPLTEGAAKAWLIKRTEPLAR"
     gene            2398..3651
                     /locus_tag="ACFM8S_001399"
     CDS             2398..3651
                     /locus_tag="ACFM8S_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HFC6314465.1"
                     /translation="MSEQHISTWKSKINALGPGIMMASAAVGGSHLIASTQAGALYGW
                     QIALIIILTNLFKYPFFRFSAHYTLDTGKSLIEGYAEKSRVYLWVFLILCILSATINA
                     GAVAIVTAAIVKMAIPSLMFDAGTVAALIMASCLIILVSGRYRALDRVSKIIIVTLSI
                     ATLAAAGIAMSRGMQMQPDFIEPTPWTLAGLGFLIALMGWMPAPIEISAINSLWVTEK
                     QRINPSEYRDGIFDFNVGYIASAVLALVFLALGAFVQYGNGEAVQMAGGKYIGQLINM
                     YAVTIGGWSRPLVAFIAFACMYGTTITVVDGYARAIAEPVRLLRGKDKTGNAEFFAWN
                     IWVAGSGLAVIFWFDGVMANLLKFAMIAAFVSAPVFAWLNYRLVKGDEKHKLTSGMNA
                     LALAGLIYLTGFTVLFLLNLAGMFK"
     gene            3806..5317
                     /gene="lysS"
                     /locus_tag="ACFM8S_001400"
     CDS             3806..5317
                     /gene="lysS"
                     /locus_tag="ACFM8S_001400"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFC6314466.1"
                     /translation="MSEQNHPQTEPQLDENQIIALRREKLHNIRQQRNAYPNDFKRDS
                     FAADLHAQYGEIGKEELDPQGIPVKVAGRMMLKRQMGKASFATIQDVSGQIQLYLNNK
                     GVSQEVLDDFNHWDLGDIVGAEGTLFKTNHGELTVRVSDIRLLSKSLRPLPDKHKGLS
                     DQETKYRQRYVDLIANEESRNTFIKRSQIIQSVRNFMVGEHYLEVETPMMHPIPGGAT
                     AKPFVTHHNALDISLYLRIAPELYLKRLVVGGLERVFEINRSFRNEGMSVRHNPEFTM
                     IEFYEAFSDYERMMQMAEDIIRNASRTVNGTAKISYNGKEVDLESPFERLTILEAIKK
                     YNPHYTDEQLNDAEWLKKEIVKHGESLPPSPGIGSLQLALFEGCAEGKLWNPTFIVDY
                     PVEVSPLARASDTKQGLTERFELFVVGRELANGYSELNDPEDQAERFKSQVAQKDAGD
                     DEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDSQTIRDVILFPQMRPE"
     gene            complement(5394..5582)
                     /locus_tag="ACFM8S_001401"
                     /pseudo
     CDS             complement(5394..5582)
                     /locus_tag="ACFM8S_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311460.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5586..6464)
                     /locus_tag="ACFM8S_001402"
     CDS             complement(5586..6464)
                     /locus_tag="ACFM8S_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HFC6314467.1"
                     /translation="MARRSKTFEEAAAEVEERFGHRGIKLVEFEGTAKPCVINCPKHG
                     NQTCSRYSNMFIGSSWGCPSCGNEQAAKAGIATLRKNHIALEMLKQAVTGMTKQERIT
                     TQAYNEMTKSVAGSNSIVLNDVQGDTTINNHHTHTHNHSDADGKALSMRLTPRPLLSD
                     RQAAAFARTGKLTGSFDLFASVVAPSQYTFAVAMPDTSMSPVIEKGDLVVVEPRMRPA
                     DEDIALIELSDKRLVVAHLVIDIAGRMLIYQTGRPSEAFDLPEGSTILGVVLEVKKRF
                     MSAAQARRRVDSDYHP"
     gene            complement(6494..7621)
                     /gene="fic"
                     /locus_tag="ACFM8S_001403"
     CDS             complement(6494..7621)
                     /gene="fic"
                     /locus_tag="ACFM8S_001403"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFC6314468.1"
                     /translation="MSNWKPDIPYNDLPPLPPKQDIESKTILKRCITARASLARLKQA
                     AELIPNQAMLINTLPVMEARASSEIENIVTTTDKLFQSLQMDTERQDPATKEALQYRT
                     ALFAGYESLTSRPLCTQTAIMVCNAIKHPYEMAIRKTGGTALKGGNSGNVVYTPPEGE
                     ETIRGKLANWERFIHESGDLDPLIIMAAAHYQFEAIHPFTDGNGRTGRILNSLLLIEK
                     GLLDLPILYLSRYIIENRADYYRLLLGVTERQDWESWIIYILDGVADTADWTVSKIDA
                     IRRLFEQTRQHIRTHAQGIYTHELVNLLFEQPYTRIANLEAAGIAKRQTASKYLKELS
                     DIGVLQEIVIGRDKLFIHPRLMELLRGEGNSFTSFQSLVKA"
BASE COUNT         1710 a         1991 c         2217 g         1984 t
ORIGIN      
        1 gtactgtctg cggcttcgcc gccttgtcct gatttttgtt aatccactat atttgcttgc
       61 ggcaagatga aaacggtgcg ggatgttttg ggaaaccaat gccgtctgaa gggctttcag
      121 acggcatttt ttgcgccgtg ctgtttaacg cgccagcggt tcggtgcgtt tgatcagcca
      181 cgctttagcc gcgccttcgg tcagcggctc gaggcggcgg cggacttcgg cgtggtagcg
      241 gttgacccag tcgatttctc cgtcggtcat cagggcggtg tccatcaggc gggtgtcgat
      301 ggggcagagg gtcagggttt caaaacagag gaagctgccg aattcggttt cctgcgggtc
      361 ggcgacggct tggttggcgg cgaggttttc aatgcggatg ccccattttc ccggacggta
      421 gaggccgggt tcgatggagg ttaccatccc ttttttcatc gcggtttcgg gcgtggcggg
      481 ggcggcgaag gcgatgcgct gcgggccttc gtggacgttg aggaaatagc ctacgccgtg
      541 tccggtgccg tggccgtagt cgcattgcgc ctgccacagg ggtttgcggc aaatcgcgtc
      601 tatcagcggt gaggggatgt tttctgggaa cacggcttcg gcaagcgcga tatgggcttt
      661 gagaacgagg gtgttgtcgc gtttttgttc ggcggtgggt gtgccgacag ggacgacgcg
      721 ggtgatgtcg gtcgtgccgc ctttgtattg cgcgccggag tcgatgagta gcagaccgtt
      781 gccgctgatg atgctgtggc tttcgggcgt tgcgctgtaa tgcggcagtg cgccgttggc
      841 gttgaagcct gcgatggtgt cgaaactcaa tgaaatgaag cctgggcgca cgctgcggtg
      901 gcgataaagc atggtgtcca cgtcgatttc ggtcaggctg ccgccgttgc cgatgatgtc
      961 ttcaaactcg gcgaagaaac cgcacaacgc cgcgccgtcg tgttccatcg cttcgcggat
     1021 gcgggcgatg tcggcttcgg atttgcagga tttgaacagc gtggacgggt tgatgccttc
     1081 aatcaggcgc atgcttttgg gcaggcgcac aagcgtgctg acggcggttt tgttcggctc
     1141 gatgagcagc acgccgccga tttgcgcgag tttgccggca acttgggcgt aaggctcgac
     1201 cgcgatgccg gcggtttgca gcgcggcggc ggcttcggcg ttcagacggc atcggtcggt
     1261 aaacaggacg gcgttgtctt tgccaatcag caggaaggac acgaaaacgg gattgaaagg
     1321 cacgtcgctg ccgcgcaggt tggtcagcca ggcgatgtcg tcaagcgagg aaaccaagtg
     1381 gtaatccgcg cctttttccg ccatgacggc gcgcacgcgg gcgagttttt cggcggcggt
     1441 ttcggagacg taggcggggt cgtggatgaa cacggtttcg gcggggaggg cgggacggct
     1501 tgtccacact tggtcaagca ggtcgtcggg gtgttcgatg cggatgtttt tggcggtcag
     1561 ggattgcgcc aaagtgcgtt tgccggtgag cgacaccata tcggaaggga tgccgacggc
     1621 ggcgttttcg ggcaggtttg ccgcgagcca ttcgttatac ggcggcactt gcccgctttt
     1681 ttgcagttcg atgccgctgc ccgcaagctg cttggcggct tgttcccaat agcggctgtc
     1741 cacccatacg cccgcttcgt cggcggtaac gacgaatgtg ccgaccgagc cggtaaagcc
     1801 cgacaattcg cgccgcgcct gccaatgttc gggcaggtat tcggacaggt gggggtcggc
     1861 ggaagggatg acgagagcgt ccaagccttg cgccttcatg gcttcgcgca atgcggacag
     1921 gtaattcgat acggtattca taacagttcc tccaatcggg ttttgcggct tcagacggca
     1981 tggacggaaa tctgaaatgc cgtctgaaaa acataaccat cttatcaaat cgccagcctg
     2041 ctgcaaaacg gacgggctga tcggatggtg cgtgtttgcg cgaaaaaagc gatgtaaggt
     2101 tgtttgttgt aaaattttag gcataagata tcgttttatg cctttttttg gaagtaatat
     2161 ggaaaatata gtcaagtttg agcatgaagt tctaatagtg tgtgttggca ggtatttgga
     2221 ttacgttaca atacgcgctt tcatcaaaat ttccctaccg agcggtatcc tgtttttgaa
     2281 gacggtagga tttgggtttg tttgggggaa tcccgcccaa attggagcaa tagatagggc
     2341 tgtgtccgaa tatgcggctc tgtgtgttga aacacggtta aaaatacgga agactttatg
     2401 tccgaacaac atatttcgac ttggaaaagt aaaatcaacg cattgggacc ggggattatg
     2461 atggcttcgg cggcggtcgg cggttcgcac ctgattgcct cgacgcaggc gggcgcgctt
     2521 tacggctggc agatcgcgct catcatcatc ctgaccaacc tcttcaaata cccgtttttc
     2581 cgcttcagcg cgcattacac gctggacacg ggcaagagcc tgattgaagg ttatgccgag
     2641 aaaagccgcg tttatttgtg ggtattcctg attttgtgca tcctctccgc cacgattaac
     2701 gcgggcgcgg tcgccattgt aaccgccgcc atcgtcaaaa tggcgattcc ctcgctgatg
     2761 tttgatgccg gcacggttgc cgccttgatt atggcatcct gcctgattat tttggtgagc
     2821 ggacgttacc gcgctttgga tcgcgtttcc aaaatcatca tcgttacttt gagtatcgcc
     2881 acgcttgctg ccgccggcat cgctatgtcg cgcggtatgc agatgcagcc cgattttatc
     2941 gagccgacac cgtggacgct tgccggtttg ggcttcctga tcgcgctgat gggctggatg
     3001 cccgcgccga ttgaaatttc cgccatcaat tctttgtggg taaccgaaaa acaacgcatc
     3061 aatccttccg aataccgcga cgggattttt gatttcaacg tcggttatat cgccagtgcg
     3121 gttttggctt tggttttcct tgcactgggt gcgtttgtgc aatacggcaa cggcgaagca
     3181 gtgcagatgg cgggcggcaa atatatcggg caattgatca atatgtacgc cgttaccatc
     3241 ggcggctggt cgcgcccgct ggtggcgttt atcgcgtttg cctgtatgta cggcacgacg
     3301 attaccgtcg tggacggcta tgcccgtgcc attgccgaac ccgtgcgcct gctgcgcgga
     3361 aaagacaaaa cgggcaacgc cgaattcttt gcctggaata tttgggtggc gggcagcggt
     3421 ttggcggtga ttttttggtt tgacggcgta atggcgaatc tgctcaaatt tgcgatgatt
     3481 gctgcttttg tgtccgcccc tgtgtttgcc tggctgaatt accgtttggt caaaggtgat
     3541 gaaaaacaca aactcacatc aggtatgaat gcccttgcat tggcaggctt gatttatctg
     3601 accggtttta ccgttttgtt cttattgaat ttggcgggaa tgttcaaatg ataacgatgc
     3661 cgtctgaaac cgcaaaccgc tttcagacgg cattgtcgcg tttatggaaa aaaatgccga
     3721 catcgcgtaa aatatgcgca aattttgtga ttcggtcagg tcgtctgaaa cagattgcgc
     3781 ctgatttatt ttttcggaaa accctatgag cgaacaaaac catccgcaaa ccgagccgca
     3841 gttggacgaa aaccaaatca tcgccctgcg ccgcgaaaaa ctgcacaaca tccgccaaca
     3901 gcgcaacgcc tatcccaacg acttcaaacg cgacagcttc gccgccgatt tgcacgccca
     3961 atacggcgaa atcggcaaag aagaactcga tccgcaaggc attcccgtca aagtggccgg
     4021 ccgcatgatg ctgaagcgtc aaatgggcaa ggcgagcttt gccaccattc aagacgtgtc
     4081 cgggcaaatc cagctttatc tgaacaacaa aggcgtgagc caagaagttt tggacgactt
     4141 caaccattgg gatttgggcg acatcgtcgg cgcggaaggc actttgttca aaaccaacca
     4201 cggcgaactg accgtacgcg tgtccgacat ccgcctgttg tccaaatcct tgcgtccgct
     4261 gccggacaaa cacaaaggct tgagcgatca ggaaaccaaa taccgtcaac gctatgtcga
     4321 tttgattgcc aatgaagaat cgcgcaatac cttcatcaaa cgcagccaaa tcatccaatc
     4381 cgtgcgtaat tttatggtgg gcgagcatta tctcgaagtc gaaaccccga tgatgcaccc
     4441 gattcccggc ggcgcgacgg caaaaccctt cgttacccac cacaatgcct tggacatttc
     4501 gctctacctg cgcatcgccc ccgagctgta tctgaaacgc ttggttgtcg gcggtttgga
     4561 acgcgtgttt gaaatcaacc gcagcttccg caacgaaggt atgtctgtgc gccacaatcc
     4621 tgaattcacc atgattgaat tttacgaagc cttctccgac tacgaacgca tgatgcaaat
     4681 ggcggaagac atcatccgca acgcatcgcg cacggtaaac ggcacggcaa aaatcagcta
     4741 caacggcaaa gaagtcgatt tggaaagccc gtttgaacgt ctgaccatcc tcgaagccat
     4801 caaaaaatac aatccgcact acaccgacga gcagttgaac gatgcggaat ggctgaaaaa
     4861 agaaatcgtc aaacacggcg aaagcctgcc gccgtccccg ggcatcggca gcctgcaact
     4921 cgcgctgttt gaaggttgcg ccgagggcaa gctgtggaat ccgaccttca tcgtcgatta
     4981 cccggtcgaa gtttccccat tggcgcgcgc ttcggatacc aaacaaggtc tgaccgaacg
     5041 tttcgaattg ttcgttgtcg gccgcgaact ggcaaacggc tattcagaat tgaatgaccc
     5101 tgaagaccaa gccgaacgct tcaaatcgca agtcgcacaa aaagacgcgg gcgacgacga
     5161 agccatgcat tacgatgccg actacatccg cgcgatggaa tttggtttgc cgccgaccgg
     5221 cggttgcggc atcggtatcg accgcttggt gatgttgctg accgattcgc aaaccatccg
     5281 cgatgtgatt ctgtttccgc agatgcgccc cgagtagtca taaaaacagt tgaaatggca
     5341 atgccgtctg aacccgattg gattcagacg gcattttgta tggcggtacg gatttattcg
     5401 ctttctaact gaccgaccca ttcggacaag gcagtcaggc gttgttcgga tttcgccacg
     5461 aacgggtttt cggtatccca cgcgtagcct gccaaaatcg aagaaagcat acggctgatt
     5521 tccgggctgc ggtcgggcgc gtacacgacg ttttggaagc ggaaatcgta ccaaccgtcc
     5581 acacatcagg ggtggtaatc cgaatcaaca cgccttctcg cctgtgcggc ggacataaac
     5641 cgttttttga cctccagcac cacacctaaa atcgtgctgc cttcgggcag gtcaaaggct
     5701 tcagacggcc tgcccgtctg ataaatcagc atcctgcccg caatatcgat aacaaggtgc
     5761 gcgacgacca gccgcttgtc ggacagttca atcagcgcga tgtcttcgtc cgcagggcgc
     5821 atacgcggct cgaccaccac caaatcccct ttttcgataa ccggcgacat ggacgtgtcg
     5881 ggcatggcaa cggcaaacgt gtactgcgag ggggcgacca ccgaagcaaa caagtcgaaa
     5941 ctgcctgtga gtttgcctgt acgggcgaaa gccgccgcct gacggtctga caacaggggg
     6001 cgtggtgtga gcctcatcga cagtgctttg ccgtcggcat cgctgtggtt gtgcgtatgc
     6061 gtatgatggt tgttgatggt cgtatcgcct tggacatcgt taaggactat gctgtttgaa
     6121 cctgccacgg atttggtcat ctcattgtag gcttgcgtcg tgatgcgctc ttgcttggtc
     6181 atacctgtta cagcctgttt cagcatttct aacgctatgt gattcttcct aagggtcgct
     6241 ataccggctt ttgcagcttg ctcattacca caagaggggc aaccccagct acttcctatg
     6301 aacatattgg agtacctcga acaggtttgg tttccatgtt tagggcagtt gattacacac
     6361 ggcttggctg taccctcaaa ctcgaccaac ttaatgccac gatgaccgaa acgttcctca
     6421 acctcagcag cagcttcttc aaatgttttt gaccttcttg ccatattgac ctccggatta
     6481 ttgattattt tggctatgct ttaacgaggg attggaatga ggtaaagctg ttgccctctc
     6541 cccgcaatag ttccattagg cgcggatgaa tgaatagttt gtccctgccg atgacgattt
     6601 cttgcagcac acctatgtct gaaagctctt tcaggtactt agaggccgtc tgccgtttgg
     6661 ctatccctgc cgcttctagg ttggcaatgc gtgtatatgg ctgctcaaac agaagattta
     6721 ccagttcgtg cgtgtagatt ccttgtgcgt gtgtccgtat gtgttgccgt gtctgctcga
     6781 acaggcggcg tatcgcatct attttcgata ccgtccaatc ggcggtatcg gctacgccgt
     6841 ctaagatgta gattatccag ctttcccaat cctgccgttc ggttacgcct aaaagcaggc
     6901 ggtaatagtc cgccctgttt tcgatgatgt agcggctcaa atacaaaata ggcaaatcca
     6961 aaagcccttt ttcaatcaat agcaggctgt tcaatatgcg ccccgtccgc ccgttgccgt
     7021 ccgtaaacgg atggatggct tcaaattggt aatgtgccgc cgccatgatg ataagcgggt
     7081 ctaaatcgcc gctttcgtga ataaaccgct cccaatttgc cagcttgccg cgtatggttt
     7141 cttctccttc gggcggggta tagacaacat ttccgctgtt gcctcctttt agggctgtgc
     7201 cgcctgtttt gcggatggcc atttcgtagg ggtgcttgat ggcgttgcag accatgatgg
     7261 cggtttgtgt gcataaaggg cggctcgtca gtgattcata gcctgcaaac agggcggtgc
     7321 ggtattgcag ggcttctttc gtggcagggt cttgccgttc cgtatccatt tgcagggatt
     7381 gaaacagctt atccgttgtg gttacgatgt tttcaatttc cgaacttgca cgggcttcca
     7441 taacaggaag ggtgttaatc agcatggctt gattcggtat cagttctgcc gcctgcttta
     7501 aacgggcaag ggatgcacgg gcggttatac aacgtttcag gatggttttg ctttcaatat
     7561 cctgttttgg cggcaggggt ggtaaatcgt tatagggaat atcgggtttc cagttgctca
     7621 tatttaaaat ttcggaaaat ttaaagatgt ttccagtata tgtttacgcc gtgtatatat
     7681 caagaatata tgtttaaaaa tttggctttt gtaaatatat gggcggtaaa accgcattat
     7741 tttatgttgt tacctatttt acaccttgtc tgcacacgat ttccaaacaa cctaaaaaga
     7801 aacctgccga aaggcaggtt taaagaggtc attttaaatg tgaactaaag tttacaccct
     7861 cggagggtat cgcgtatatc agacgtactt aatatacact at
//
LOCUS       DAXHSF010000095         7859 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.095, whole genome shotgun sequence.
ACCESSION   DAXHSF010000095 DAXHSF010000000
VERSION     DAXHSF010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7859
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.095"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(<1..335)
                     /locus_tag="ACFM8S_001404"
     CDS             complement(<1..335)
                     /locus_tag="ACFM8S_001404"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314469.1"
                     /translation="MPSELPFQTALCPNDSISSVFSLSIIRYAQITATQADTVPIASK
                     KCCQSVIGGTGQRPHSISAPPNNRYSSISAIQSNNGYFKRCNQKTNHVRTICLPHSDN
                     DIVPLPCNV"
     gene            complement(358..1659)
                     /locus_tag="ACFM8S_001405"
     CDS             complement(358..1659)
                     /locus_tag="ACFM8S_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HFC6314470.1"
                     /translation="MPSETARPLYADTRAAHTLVWFRQNLRIRDNAALCAAVAEGLPV
                     IGIWIDDAETDNPRRAAFYRQSAAELAQGLAGRGIPLYTAASPAELVRLAVRLNIRAV
                     IADESHTFADKLADNALWHELDKHGIALTFVNDRAVFGKTDLIPDGGTAHTDFDRYRE
                     VWLDRFSKQPPAGPDLFAAYRQPFPENLSAPPPAALSDGIFLPQNSGETAAWRQWRRF
                     LEQADSYSVLKDFPSRKHTSLMGAYLSAGCISPRLLARESLERRLNAWADNIIRRDFF
                     LQLALQYADDDPSDGNPEHTLRLTLWQQGQTGIPIIDAAMRCLHKTGSLHPALRRLSA
                     DFFCHVLNLPRREGEIWFARQLTDFDAAINQGNWRLAASRHTCPDIAAASYRTDPDGT
                     FIKRHIPELAHLSADTVHTPWRFACSVDTHGYPAHPVAGIP"
     gene            complement(1649..2560)
                     /locus_tag="ACFM8S_001406"
     CDS             complement(1649..2560)
                     /locus_tag="ACFM8S_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFC6314471.1"
                     /translation="MDTLFSLKVFRQVVQSGGFTRAADALGISTAMASKHVSHLENTV
                     QAKLLHRNSRNLSLTEAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWF
                     AGSQICNWLAEYRERYPEVALELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAE
                     IEFALLASPDFLRRNGVPETPEEVAGLPAVLPTYTNQQKLDLTRKSDGKKYRLELTPV
                     IRTDNTLMMREMIKAGACIGYQPLWAAEHDLRCGSLVRLLPEYTVPTDRLNAVYADRA
                     FLSAKVRSFIDFLNEKIASRKGCRNAV"
     gene            2668..3066
                     /locus_tag="ACFM8S_001407"
     CDS             2668..3066
                     /locus_tag="ACFM8S_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFC6314472.1"
                     /translation="MSDCCNRIQPVLLSVLRIVTAYLFLLHGTSKIFAFPIEMGSGSP
                     GGLLLLAGILEIVGGILLVLGLFARPAAFVLSGQMAVAYFMAHASGNALFPIANGGES
                     AVLFCFVFLYIAAAGGGAWSLDRLFFKRKA"
     gene            3115..3720
                     /locus_tag="ACFM8S_001408"
     CDS             3115..3720
                     /locus_tag="ACFM8S_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFC6314473.1"
                     /translation="MTRQSLQQAAESRRSIYSLNKNLPVGKDEVVQIVEHAVLHTPSS
                     FNSQSARVVVLFGEEHDKVWQFVEDALRAVVPADSFEPTAQKLKLFKAGAATILFYED
                     QNVVKGLQEQFPAYAANFPVWADQANAMVQYAVWTTLAAAGAGANLQHYNPLPDAAIA
                     KAWNIPENWLLRAQMVIGGIEGAAGEKTFEPVAERLKVFGA"
     gene            3906..6017
                     /locus_tag="ACFM8S_001409"
     CDS             3906..6017
                     /locus_tag="ACFM8S_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFC6314474.1"
                     /translation="MKISFHLALLPTLIIASFPVAAADTQDNGEHYTATLPTVSVVGQ
                     SDTSVLKGYINYDEAAVTRNGQLIKETPQTIDTLNIQKNKNYGTNDLSSILEGNAGID
                     AAYDMRGESIFLRGFQADASDIYRDGVRESGQVRRSTANIERVEILKGPSSVLYGRTN
                     GGGVINMVSKYANFKQSRNIGAVYGSWANRSLNMDINEVLNKNVAIRLTGEVGRANSF
                     RSGIDSKNVMVSPSITVKLDNGLKWTGQYTYDNVERTPDRSPTKSVYDRFGLPYRMGF
                     AHRNDFVKDKLQVWRSDLEYAFNDKWRAQWQLAHRTAAQDFDHFYAGSENGNLIKRNY
                     AWQQTDNKTLSSNFTLNGDYTIGRFENHLTVGMDYSREHRNPTLGFRRNFTASIDPYD
                     RASWPASGRLQPILTQNRHKADSYGIFVQNIFSATPDLKFVLGGRYDKYTFNSENKLT
                     GSSRQYSGHSFSPNIGAVWNINPVHTLYASYNKGFAPYGGRGGYLSINTSSSAVFNAD
                     PEYTRQYETGVKSSWLDDRLSTTLSAYQIERFNIRYRPDAENNPYTWAVGGKHRSRGV
                     ELSAIGQIIPKKLYLRGSLGVMQAKVVEDKKNPDRVGIHLNNTSNVTGNLFFRYTPTE
                     NLYGEIGVTGTGKRYGYNSRNKEVTTLPGFARVDAMLGWNHKNVNVTFAAANLFNQKY
                     WRSDSMPGNPRGYTARVNYRF"
     gene            6215..6874
                     /locus_tag="ACFM8S_001410"
     CDS             6215..6874
                     /locus_tag="ACFM8S_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFC6314475.1"
                     /translation="MKTAILIDGAFFIKRVRSLGLSQNPHDAKRIADLAFRYSMLHLK
                     DKINGTDQMDDLYRIFFYDCVPFEKKMHNPVSHRAIDFSKTPEAIFRCNLHTELKKKR
                     KLALRLGKLSDNTAWILKPQVMKNLLKNPSTQITENDVVLDVKQKGVDMRIGLDISSI
                     TLKKQADKIILFSGDSDFVPAAKLARREGIDFILDPMWNHVPPDLMEHIDGLKTKCPK
                     P"
     gene            7045..7740
                     /locus_tag="ACFM8S_001411"
     CDS             7045..7740
                     /locus_tag="ACFM8S_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFC6314476.1"
                     /translation="MKLKTLALTSLTLLALAACSKQAETSVPADSAQSSSSAPAAPAE
                     LNEGVNYTVLSTPIPQQQAGKIEVLEFFGYFCPHCAHLEPVLSEHIKTFKDDTYMRRE
                     HVVWGDEMKPLARLAAAVEMAGESDKANSHIFDAMVNQKINLADTDTLKKWLSEQTAF
                     DGKKVLAAFEAPESQARAAQMEELTNKFQISGTPTVIVGGKYQVEFKDWQSGMTTIDQ
                     LVDKVREEQKKPQ"
BASE COUNT         1927 a         1981 c         2127 g         1824 t
ORIGIN      
        1 ttaacattgc acggaagcgg tacaatatcg ttgtcggaat gggggaggca aatcgtgcgg
       61 acgtggttgg ttttttggtt gcagcgtttg aaatacccgt tgttgctttg gattgcggat
      121 atgctgctgt accggttgtt gggcggcgcg gaaatcgaat gcggccgttg ccctgtgccg
      181 ccgatgacgg attggcagca ttttttgctg gcgatgggaa cggtgtcggc ttgggtggcg
      241 gtgatttggg catatctgat tattgaaagt gaaaaaacgg aagatattga gtcattcgga
      301 cacaatgccg tctgaaacgg aagttcagac ggcatttgtt ttaggttgcc gtgccgctta
      361 gggaataccg gcgacaggat gggcgggata gccgtgggta tcgaccgaac aggcaaaccg
      421 ccaaggcgtg tggacggtgt cggcggacag gtgggcgagt tcgggaatgt gccgtttgat
      481 aaaagtaccg tccgggtcgg tacggtatga ggcggcggca atgtcgggac aggtgtgccg
      541 tgaggcggca agccgccagt tgccttggtt gattgccgca tcgaaatcgg tcagctgtcg
      601 ggcaaaccat atctcgcctt cgcggcgggg gaggtttaaa acgtggcaga aaaagtccgc
      661 gctcaagcgt ctcagggcgg ggtggaggct gccggttttg tgcaaacagc gcatcgcggc
      721 atcgataatc ggaatgccgg tctggccctg ctgccaaagc gtcaggcgca gggtgtgttc
      781 cggattgccg tctgaagggt cgtcatccgc gtactgcaag gcaagttgaa ggaaaaaatc
      841 gcggcggatg atgttgtccg cccacgcgtt cagacggcgt tcgaggcttt cccgcgcgag
      901 caggcgcggc gagatgcagc cggcactcaa atacgcgccc atcagcgaag tgtgtttgcg
      961 cgaggggaaa tcctttaaaa cggagtagga atccgcctgt tcgagaaacc gccgccactg
     1021 ccgccaagcc gccgtttcgc cgctgttttg cggcaggaag atgccgtctg aaagggcggc
     1081 aggcggcggg gcggaaaggt tttcggggaa gggttggcgg tatgccgcga ataggtccgg
     1141 accggcgggg ggctgcttgg aaaagcggtc gagccatact tcgcggtagc ggtcgaaatc
     1201 ggtgtgtgcc gtgccgccgt cgggtatcag gtcggttttg ccgaaaacgg cgcggtcgtt
     1261 gacgaaggtt aacgcgatgc cgtgtttgtc caattcgtgc caaagggcgt tgtcggcgag
     1321 tttgtcggca aaagtatggg attcgtcggc gatgacggcg cggatattga ggcggacggc
     1381 gagccggacg agctcggcag gcgatgccgc cgtgtagagc gggatgccgc gccctgcaag
     1441 cccttgggcg agttcggcgg cggattggcg gtagaacgcg gcgcggcgag ggttgtctgt
     1501 ttcggcatcg tcaatccaaa taccgataac gggcaaacct tcggcaacgg cggcgcataa
     1561 ggcggcgttg tcgcggatgc ggaggttttg gcggaaccag acgagcgtgt gtgcggcgcg
     1621 cgtgtccgca taaagggggc gggcggtttc agacggcatt tcggcagcct ttcctgctgg
     1681 cgattttttc gttcagaaaa tcgatgaagc tgcggacttt cgcgcttaag aacgccctgt
     1741 ccgcataaac ggcattcagg cggtcggtcg ggacggtata ttcgggcagc agcctcacca
     1801 gcgagccgca gcgcaaatcg tgttccgccg cccaaagcgg ctgataaccg atgcacgcgc
     1861 ccgccttaat catttcgcgc atcatcagcg tgttgtcggt acggatgacg ggggtcagtt
     1921 caagccggta ttttttgccg tccgatttgc gggtgaggtc gagtttctgc tggttggtgt
     1981 aggtcggcag gacggcgggc agccccgcca cttcttccgg cgtttccggc acgccgttgc
     2041 gcctcaggaa atcgggcgag gcgagcaggg caaattcgat ttccgccagt gggcgcgcaa
     2101 tcagcgacgg ggacagggtt tgggaaacgc gcaacgccaa atccacgcct tcggcaatca
     2161 aatcgacgtg gcggttgtcc aaaatcagtt ctaatgccac ttcgggataa cgttcgcggt
     2221 attccgccag ccagttgcat atctggctgc cggcaaacca cagcggcatc gttacgcgca
     2281 gcagcccctg cggtttttcc gtccctccgg cggctttttg cgcggcatcg tcgagcgtgt
     2341 cgagcgcgta actgcattgc cggtagtatt cttccccggc ttcggtcagg ctgaggttgc
     2401 ggctgttgcg gtgcaggagt ttggcttgga cggtgttttc caagtggctg acgtgtttgc
     2461 ttgccattgc ggtggagatg ccgagcgcgt cggcggcgcg ggtgaagccg ccgctttgga
     2521 cgacttggcg gaaaaccttg aggctgaaca gggtgtccat attttcttgt gtggaaaagt
     2581 tgtatcaata aaagcagtat atatttgaaa aggggaaaca tctatactct accgcctgaa
     2641 atgaagacaa atatcaaagg agcttttatg tccgattgct gcaaccgtat ccaaccggtt
     2701 ttgctttctg ttttgcgtat cgtaaccgct tacctgtttt tgttgcacgg tacgtcgaaa
     2761 atcttcgcct tccccattga aatgggcagc ggttcgcccg gcgggctgtt gctgcttgcc
     2821 ggtattttag aaattgtcgg cggcattttg ctggtgttgg gcctgtttgc gcgccctgcc
     2881 gcgtttgttt tgtccggcca gatggcggtt gcctatttta tggcgcacgc ttccggaaat
     2941 gctttgttcc cgattgccaa cggcggcgag tccgcagtgc tgttctgctt cgtattcctc
     3001 tatatcgcgg cagcgggcgg cggagcatgg tcgctggaca ggctgttttt caagcgtaaa
     3061 gcctgaatcg gactgcctaa agtgtatttt gttgaatgtt tttgaggaaa agaaatgacc
     3121 cgtcaatctc tgcaacaggc tgccgaaagc cgccgttcca tttattcgtt aaataaaaat
     3181 ctgcccgtcg gcaaagatga agttgtccaa atcgtcgaac acgccgtttt gcacacacct
     3241 tcttcgttca attcccaatc tgcccgcgtg gtcgtgctgt ttggcgaaga gcatgataag
     3301 gtgtggcaat ttgtcgaaga cgcgctgcgt gccgtcgtgc ctgccgacag ttttgaaccg
     3361 accgcgcaaa aattgaagct gtttaaggcg ggtgcggcaa ctattttgtt ttatgaagat
     3421 caaaatgttg tcaaaggttt gcaggagcag ttccctgctt atgccgccaa cttccccgtt
     3481 tgggcggatc aggcgaacgc gatggtacag tatgccgtct ggacgacact tgccgccgcc
     3541 ggagcaggcg caaacctgca acactataat ccgctgcccg atgcggcgat tgccaaagcg
     3601 tggaatatcc ccgaaaactg gctgttgcgc gcgcaaatgg ttatcggcgg tattgaaggg
     3661 gcggcaggtg aaaagacctt tgaaccagtt gcagaacgtt tgaaagtgtt cggcgcataa
     3721 tttcgcggtc aaaaaaatgc cgtctgaacc ctgttcagac ggcatttttc agtatcaggc
     3781 ggcgagtttt ccgcattctg agacctttgt ttacaaatat catgttcaat atagttaaaa
     3841 gaaattattc tcatttcctc cgtgaggcaa tataattcgg ttgttttgtt aaattgagta
     3901 taagaatgaa aatatcattt catttagctt tattacccac gctgattatt gcttccttcc
     3961 ctgttgctgc cgccgatacg caggacaatg gtgaacatta caccgccacg ctacctaccg
     4021 tttccgtggt cggacagtcc gacaccagcg tactcaaagg ctacatcaac tacgacgaag
     4081 ccgccgttac ccgcaacgga cagctcatca aagaaacgcc gcaaaccatc gatacgctca
     4141 atatccagaa aaacaaaaat tacggtacga acgatttgag ttccatcctc gaaggcaatg
     4201 ccggcatcga cgctgcctac gatatgcgcg gcgaaagcat tttcctgcgc ggttttcaag
     4261 ccgatgcatc cgatatttac cgcgacggcg tgcgcgaaag cggacaagtg cgccgcagta
     4321 ctgccaacat cgagcgcgtg gaaatcctga aaggcccgtc ttccgtgctt tacggccgca
     4381 ccaacggcgg cggcgtcatc aacatggtca gcaaatacgc caacttcaaa caaagccgca
     4441 acatcggtgc ggtttacggt tcgtgggcaa accgcagcct gaatatggac attaacgaag
     4501 tgctgaacaa aaacgtcgcc atccgtctca ccggcgaagt cgggcgcgcc aattcgttcc
     4561 gcagcggcat agacagcaaa aatgtcatgg tttcacccag cattaccgtc aaactcgaca
     4621 acggcttgaa atggacgggg caatacacct acgacaatgt ggagcgcacg cccgaccgca
     4681 gtccgaccaa gtccgtgtac gaccgcttcg gactgcctta ccgcatgggg ttcgcccacc
     4741 ggaacgattt tgtcaaagac aagctgcaag tttggcgttc cgaccttgaa tacgccttca
     4801 acgacaaatg gcgtgcccaa tggcagctcg cccaccgcac ggcggcgcag gattttgatc
     4861 atttctatgc aggcagcgaa aatggcaact taatcaaacg taactacgcc tggcagcaga
     4921 ccgacaacaa aaccctgtcg tccaacttca cgctcaacgg cgactacacc atcggccgtt
     4981 ttgaaaacca cctgaccgta ggcatggatt acagccgcga acaccgcaac ccgacattgg
     5041 gtttcagacg caactttacc gcctccatcg atccatacga ccgcgcaagc tggccggctt
     5101 cgggcagatt gcagcctatt ctgacccaaa accgccacaa agccgactcc tacggcatct
     5161 tcgtgcaaaa catcttctcc gccacgcccg atttgaaatt cgtcctcggc ggtcgttacg
     5221 acaagtacac ctttaattcc gaaaacaaac tcaccggcag cagccgccag tacagcggac
     5281 actcgttcag ccccaacatc ggtgcagtgt ggaacatcaa tcccgtccac acactttacg
     5341 cctcgtataa caaaggcttc gcgccttatg gcggacgcgg cggctatttg agcatcaaca
     5401 cgtcgtcttc cgccgtgttc aacgccgacc ccgagtacac ccgccaatac gaaaccggcg
     5461 tcaaaagcag ttggctggac gaccgcctca gcaccacatt gtccgcctac caaatcgaac
     5521 gcttcaatat ccgctaccgc cccgacgcgg aaaataatcc ctacacttgg gcagtcggcg
     5581 gcaaacaccg ttcgcgtggc gtggaattgt ccgccatcgg gcaaatcatc cccaaaaaac
     5641 tctatctgcg cggttcgttg ggcgtgatgc aggcgaaagt cgttgaagac aaaaaaaatc
     5701 ccgaccgagt gggcatccat ttgaataata ccagcaacgt taccggcaac ctgtttttcc
     5761 gttatacacc gaccgaaaac ctctacggcg aaatcggcgt aaccggtaca ggcaaacgct
     5821 acggttacaa ctcaagaaat aaagaagtga ctacgcttcc aggctttgcc cgagttgatg
     5881 ccatgcttgg ctggaaccat aaaaatgtta acgttacctt tgccgcagcc aatctgttca
     5941 atcaaaaata ttggcgttcg gactctatgc cgggtaatcc gcgcggctat actgcccggg
     6001 taaattaccg tttctgatga aatcaggcaa aggccgtctg aaaatcctga taatccgtaa
     6061 attcagcata cgaacttttt gacaggcttt atctacatca gtataatcca aaaagaaata
     6121 gcgcccttgg cagtacgcat tacccattaa atcatagcgg gtatgccgat cccaagggct
     6181 tttttatatc tttgacaaat ataggggaag caatatgaaa accgccattt tgattgacgg
     6241 tgcatttttt attaagcggg tacggtcatt aggcctgtct caaaatccac acgatgcaaa
     6301 aagaattgct gatttggctt tccgatattc gatgctgcat ctgaaagata aaatcaacgg
     6361 cacagatcaa atggatgatt tatatagaat tttcttttac gactgcgtgc cgtttgaaaa
     6421 gaaaatgcac aatcccgtat ctcatcgtgc catagatttt tcaaagactc cggaagccat
     6481 atttcgttgc aatctgcata ccgaattgaa gaagaagcgt aaattagcgt tacgtttagg
     6541 caagctgtcg gacaatacag catggatatt aaaaccccaa gtcatgaaaa atcttctgaa
     6601 aaacccgtca actcaaatta cggaaaacga tgtcgtgctc gatgttaaac aaaaaggtgt
     6661 agatatgcgt ataggcttgg atatttcatc tattacctta aaaaaacaag ccgataaaat
     6721 catcttgttt tctggtgatt ccgattttgt cccagcagcc aaattagcca gacgggaagg
     6781 tatcgatttt attcttgatc ctatgtggaa tcatgtccca cctgacttaa tggaacacat
     6841 cgacggattg aagacaaaat gtccgaaacc gtaaattact agaacaaagg ccgtctgaaa
     6901 ataaggctaa ttatctaagc catatttcag acggccttta taaaggctga aataaaacta
     6961 aacacatttt ttcactcaaa tcgaacacgc cttcaataaa atgccataaa atccgcacat
     7021 taatctgaca cacaagagat acctatgaaa ctgaaaacct tagctttgac ttcattgacc
     7081 ctgttggcat tggccgcttg tagcaaacag gctgaaacca gtgttccggc agacagcgcc
     7141 caaagcagct catctgctcc ggcagcccct gctgagttga acgaaggtgt gaactacact
     7201 gtattgtcta cgcctattcc gcaacagcag gccggtaaaa tcgaagtatt ggaatttttc
     7261 ggctacttct gcccgcattg cgcccatctt gagccggtct tgagcgagca catcaaaacg
     7321 tttaaagacg atacctatat gcgccgggag catgtcgtgt ggggtgatga aatgaaacct
     7381 ttggcacgtt tggcggccgc agtggaaatg gccggtgaat cagataaagc caacagccat
     7441 attttcgatg cgatggttaa tcaaaaaatc aatctggccg ataccgatac cctgaaaaaa
     7501 tggctgtccg agcaaacagc gtttgacggc aaaaaagtat tggctgcatt tgaggctcct
     7561 gaaagccaag cgcgtgcggc tcaaatggaa gagttgacca ataaattcca aatcagcggc
     7621 acaccgactg tgattgtcgg cggcaaatac caagttgaat ttaaagactg gcagtccggt
     7681 atgaccacga ttgaccagtt ggtggataaa gtacgcgaag agcagaaaaa gccgcaataa
     7741 gttgaggatt gaatgagtaa aggccatctg aaaataggat ttcagacggc cttttgtatt
     7801 taggctttat agaagagatg attgcttaaa gccttatggt tttaaatcag aatatatag
//
LOCUS       DAXHSF010000096         7827 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.096, whole genome shotgun sequence.
ACCESSION   DAXHSF010000096 DAXHSF010000000
VERSION     DAXHSF010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7827
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.096"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(205..2808)
                     /gene="pepN"
                     /locus_tag="ACFM8S_001412"
     CDS             complement(205..2808)
                     /gene="pepN"
                     /locus_tag="ACFM8S_001412"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFC6314477.1"
                     /translation="MSKTVHYLKDYQTPAYHILKTDLHFDINEPQTIVKSRLTVEPQR
                     VGEPLVLDGSAKLLSVKINGAAADYVLEGETLTIADVPSERFTVEVETEILPAENKSL
                     MGLYASGGNLFTQCEPEGFRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDG
                     GGFSDGRHWVKWEDPFAKPSYLFALVAGDLAVTEDCFTTMGGRNVKIEFYTTEADKPK
                     VGFAVESLKNAMKWDETRFGLEYDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADS
                     RTATDTDFEGIESVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRAGRA
                     VRRIENIRLLRQHQFPEDAGPTAHPVRPARYEEMNNFYTMTVYEKGAEVVRMYHTLLG
                     EEGFQKGMKLYFQRHDGQAVTCDDFRAAMADANGINLDQFALWYSQAGTPVLEAEGRL
                     KNNVFELTIKQTVPPTPDMADKQPMMIPVKIGLLNRNGEAVAFDYQGKRATEAVLLLT
                     EAEQTFTLEGVTEAVVPSLLRGFSAPVHLNYPYSDDDLLLLLAHDSDAFTRWEAAQTL
                     YRRAVAANLAALSDGIGLPKHEKLLAAVEKVISDGLLDNAFKALLLGVPSEAELWDGA
                     ENIDPLRYHQAREALMDTLAVRFLPKWHELNRQAAKQENQSYEYSPEAAGWRTLRNVC
                     RAFVLCADPAHIETVAEKYGEMAQNMTHEWGILSAVNGNESDTRNRLLAQFADKFSDD
                     ALVMDKYFALVGSSRRSDTLQQVQTALQHPKFSLENPNKARSLIGSFSRNVPHFHAED
                     GSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNLVKQALQRIRAQEGLSK
                     DVGEIVGKILD"
     gene            complement(2940..3734)
                     /locus_tag="ACFM8S_001413"
     CDS             complement(2940..3734)
                     /locus_tag="ACFM8S_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFC6314478.1"
                     /translation="MNTILAFDIETVPDVQGIRTLYHLPSDLPDDEVVLFAQQKRRAQ
                     TGGDFMQHHLHQVVAVSCCMRWGQDKIHIGTIGEADDGEEVVIAKFFELVEKHTPQLV
                     SWNGGGFDLPVLHYRSLIYGINAARYWDMGEGEFGDSRDFKWNNYISRYHQRHCDLMD
                     LLALYQPRANVPLDDMAKLCGFPGKLGMDGSKVWEAFHTGRLKEIRNYCETDAANTYL
                     MYLRFCLVSGRLDADEYEMEIKRMRNYLSAQAGEKPHWEEFVRAWE"
     gene            complement(3850..3984)
                     /locus_tag="ACFM8S_001414"
     CDS             complement(3850..3984)
                     /locus_tag="ACFM8S_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314479.1"
                     /translation="MPSERMFRPVFGLSGVLPETAGRKFAGAGLSCAGKAAGESLYCL
                     "
     gene            complement(4341..4685)
                     /locus_tag="ACFM8S_001415"
     CDS             complement(4341..4685)
                     /locus_tag="ACFM8S_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314480.1"
                     /translation="MNLEDYKDILIKLLFHHNNLVNEYSYFIGNKNLFKIVSRTKTSK
                     GFFFTIEKPLNFLSKKTYFEFNFKYLHLGKECFGSFMCWINTNLIEFEGVFFNDSLPD
                     IMIINNFFEIND"
     gene            complement(4907..5797)
                     /locus_tag="ACFM8S_001416"
     CDS             complement(4907..5797)
                     /locus_tag="ACFM8S_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HFC6314481.1"
                     /translation="MKFIFFVLYVLQFLPFALLHKLADLTGLLAYLLVKPRRRIGEIN
                     LAKCFPEWDGKKRETVLKQHFKHMAKLMLEYGLYWYAPAKRLKSLVRYRNKHYLDDAL
                     AAGEKVIILYPHFTAFEMAVYALNQDVPLISMYSHQKNKILDEQILKGRNRYHNVFLI
                     GRTEGLRALVKQFRKSSAPFLYLPDQDFGRNDSVFVDFFGIRTATITGLSRIAALANA
                     KVIPAIPVREADNTVTLQFYPAWKSFPGEDAKADAQRMNRFIEERVREHPEQYFWLHK
                     RFKTRPEGSPDFYGLHEVTK"
     gene            complement(5848..6384)
                     /gene="ruvC"
                     /locus_tag="ACFM8S_001417"
     CDS             complement(5848..6384)
                     /gene="ruvC"
                     /locus_tag="ACFM8S_001417"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFC6314482.1"
                     /translation="MAASVRILGIDPGSRVTGFGIIDVRGRDHFYVASGCIKTPADAP
                     LADRIAVIVRHIGEVVAVYKPQQAAVEQVFVNVNPASTLMLGQARGAALAALVSHKLP
                     VSEYTALQVKQAVVGKGKAAKEQVQHMVVQMLGLSGTPQADAADGLAVALTHALRNHG
                     LAAKLNPSGMQVKRGRFQ"
     gene            complement(6387..6626)
                     /locus_tag="ACFM8S_001418"
     CDS             complement(6387..6626)
                     /locus_tag="ACFM8S_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HFC6314483.1"
                     /translation="MPHTLPDISQCIRQNLEQYFKDLNGTEPCGVYDMVLHQVEKPLL
                     VCVMEQCGGNQSKASVMLGLNRNTLRKKLIQHGLL"
     gene            complement(6657..7667)
                     /gene="dusB"
                     /locus_tag="ACFM8S_001419"
     CDS             complement(6657..7667)
                     /gene="dusB"
                     /locus_tag="ACFM8S_001419"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFC6314484.1"
                     /translation="MHIGGYFIDNPIALAPMAGITDKPFRRLCRDFGAGWAVCEMLTS
                     DPTLRNTRKTLHRSDFADEGGIVAVQIAGSDPQQMADAARYNVSLGAQLIDINMGCPA
                     KKVCNVQAGSALMQNEPLVAAILEAVVRAAGVPVTLKTRLGWHDDHQNLPVIAKIAED
                     CGIAALAVHGRTRTQMYKGEARYELIAETKCRLNIPVWVNGDITSPQKAQAVLKQTAA
                     DGIMIGRGAQGRPWFFRDLKHYAEHGVLPPALSLAECAAAILNHIRAIHAFYGDSAGV
                     RIARKHIGWYIDEMPDGEQTRREINRLDSAAAQYDTLAGYLERLAGKTDIWACAYRQN
                     AV"
BASE COUNT         1737 a         2057 c         2033 g         2000 t
ORIGIN      
        1 acaaaaacca gtacagcgtt gcctcgcctt accgtactat ctgtactgtc tgcggcttcg
       61 tcgccttgtc ctgatttttg ttaatccact ataattaata tgactttgcg gccgttttgc
      121 cattgcgtaa taaaacgatg gggaagtgat gataaaacgt gtgtgtaact atatcagacg
      181 gcattgtttt tctgtttgac ggcctcaatc caaaattttg ccgacgattt cgcccacgtc
      241 tttcgacaat ccttcctgcg cccgaatgcg ctgcaatgct tgtttcacca agtttttgcg
      301 gtgcggctcg agcttgttgc agaggttgaa cgcctgcacc aggcgggcgg cgacctgcgg
      361 gttaaagcgg tcgatttcga tgactttgtc ggcgatgaag cggtagccgc tgccgtcttc
      421 tgcgtggaaa tgcgggacgt tgcggctgaa gctgccgatg agcgagcggg ctttgttggg
      481 attttcgagg ctgaacttcg gatgctgcaa ggcggtttga acctgttgca gggtgtcgct
      541 gcggcggctt gagccgacga gggcgaaata tttgtccatc accagcgcgt cgtctgaaaa
      601 cttgtcggca aactgagcca gcaggcggtt gcgcgtatcg ctttcgttgc cgttaacggc
      661 ggacaggatg ccccattcgt gggtcatgtt ttgcgccatt tcgccgtatt tttcggcaac
      721 ggtttcgatg tgcgcggggt cggcgcacag gacgaaggcg cggcagacgt tgcgcagcgt
      781 gcgccagccg gcagcttcgg ggctgtactc gtagctttgg ttttcctgct tcgccgcctg
      841 acggttcaat tcgtgccatt tcggcaggaa gcggacggca agcgtatcca tcagggcttc
      901 gcgcgcctga tggtagcgca gcgggtcgat gttttccgcg ccgtcccaca gctcggcttc
      961 ggacggcacg cccaaaagca gggctttgaa ggcgttgtcc aagaggccgt ctgaaatgac
     1021 tttttcgacg gcggcaagca gtttttcgtg tttcggcaac ccgatgccgt ctgaaagcgc
     1081 ggcaaggttg gcggcgacgg cgcggcggta gagcgtttgg gcggcttccc agcgcgtgaa
     1141 ggcgtcgctg tcatgggcga gcaggagcag caggtcgtcg tcgctgtatg gatagttcag
     1201 gtgcaccggc gcgctgaacc ctcgcagcag cgagggaacg acggcttcgg ttacgccttc
     1261 gagcgtgaag gtctgttcgg cttcggtcag cagcaatacg gcttcggttg cgcgtttgcc
     1321 ctgataatca aatgccaccg cttcgccgtt gcggttcagc agcccgattt tgacgggaat
     1381 catcatcggt tgcttgtccg ccatatcggg cgtaggcggc acggtttgtt tgatggttaa
     1441 ctcgaacaca ttgtttttca gacggccttc ggcttccaaa acgggcgtgc ccgcctggct
     1501 gtaccacaag gcgaactggt cgaggttgat gccgttcgcg tccgccatcg ccgcgcggaa
     1561 atcgtcgcag gtcacggcct gcccgtcgtg gcgttggaaa tagagcttca tacctttctg
     1621 gaagccctct tcaccgagca gggtatgata catccgcacc acttccgcgc ctttttcata
     1681 aacggtcatg gtgtaaaaat tgttcatctc ctcatagcgg gcggggcgca ccggatgtgc
     1741 ggtcggacct gcgtcttcgg ggaactggtg ctggcgcagc aggcggatgt tttcgatacg
     1801 gcgcacggcg cggccggcgc ggtcgccgga aaactcttgg tcgcggaaca cggtcaaccc
     1861 ttccttcagc gaaagctgga accagtcgcg gcaggttacg cggttgcccg tccagttgtg
     1921 gaaatattcg tgtccgacca cggattcgat gccttcgaaa tcggtatcgg tggcggtgcg
     1981 gctgtcggcg aggacgaact tggtgttaaa gatgttcaaa cccttgtttt ccatcgcacc
     2041 catattgaaa tcgcccacgg cgacgaccat gaaaatatcc aagtcgtatt ccaaaccgaa
     2101 gcgcgtttcg tcccatttca tcgcattttt cagcgattcc acggcaaagc cgaccttggg
     2161 cttgtccgct tcggtggtgt aaaactcgat tttgacgttt ctgccgccca ttgtggtgaa
     2221 acagtcttcc gtgaccgcca aatcgcccgc gaccaaagca aacagataac tcggtttggc
     2281 aaacgggtct tcccatttca cccaatggcg gccgtctgaa aacccgccgc cgtcgatttt
     2341 gttgccgttg gagagcaaaa cgggatagcg ttttttgtct gcgacgatgg tggttgtgaa
     2401 cttggacatc acatctggac ggtcgatgta gaacgtaatt ttgcggaagc cctccggctc
     2461 gcactgggta aacagattgc cgccggaagc atacagcccc atcagcgatt tgttttccgc
     2521 cggcaggatt tcggtttcca cttcgacggt gaagcgttcg gacggcacgt ctgcaatcgt
     2581 cagcgtttcg ccttccaaca cataatccgc cgccgccccg ttgattttga cggacaagag
     2641 tttcgccgaa ccgtccaaca ccagcggttc ccccaccctc tgcggctcga ccgtcaaacg
     2701 cgacttcaca atggtttgcg gttcgttaat atcaaaatgt aaatcggttt tgagaatatg
     2761 gtaggcgggc gtttgatagt ctttgagata atgcacggtt ttgctcattt ttttctttca
     2821 atgttatttt gtttgactgg aaaaggcttc agacggcacg ggagcatccc gcgtatgccg
     2881 tctgaagccg cagcggcggc acgggcgcgc cgccggacaa ccggtttgaa tttcaatctt
     2941 tattcccacg cgcggacaaa ctcttcccaa tgcggctttt ccccggcttg tgcggacagg
     3001 taattccgca tccgtttgat ttccatttcg tattcgtccg catccagcct gccgctgacg
     3061 aggcagaaac gcaggtacat cagataagtg tttgccgcgt cggtttcgca ataattgcgg
     3121 atttccttca gcctgcccgt atggaatgcc tcccacacct tgctgccgtc catacccagc
     3181 ttgcccggaa aaccgcacag tttcgccata tcgtccagcg gcacgtttgc cctcggctgg
     3241 taaagcgcga gcaaatccat caaatcgcag tggcgttggt gatagcggct gatgtagttg
     3301 ttccacttga aatcgcggct gtcgccaaat tccccttcgc ccatatccca ataacgcgcg
     3361 gcattgatgc cgtatatcag ggagcggtaa tgcagtacgg gcaggtcgaa cccgccgccg
     3421 ttccaactga ccagttgcgg cgtatgtttt tccaccaact cgaaaaattt ggcgataacc
     3481 acttcttcgc cgtcgtccgc ctcgccgatt gtgccgatat ggattttgtc ctgcccccag
     3541 cgcatacagc acgaaaccgc cacaacctga tggagatgat gctgcataaa atccccgccc
     3601 gtctgggctc ggcgtttttg ctgggcaaac agcaccactt catcgtcggg caaatcggac
     3661 gggagatgat ataaagtccg tatcccttgc acgtcgggca cggtttcaat atcgaaggcc
     3721 aaaatcgtat tcatcgctgc accttgtatt caaaaaggac ggatggctat tgtgtcatta
     3781 aaaggcaggt aaaaaaagag gcaacccccc acaggattgc cccaatacct caaatcagag
     3841 atttacgctt cacaaacaat acaggctttc gcctgcggct ttacctgcgc agctcaaccc
     3901 tgcgccggca aactttcgtc ccgccgtttc cggcaagaca ccggacagcc cgaagaccgg
     3961 ccggaacatc ctttcagacg gcattgtttc gctgcgtctg taattccgtg tagcggaatg
     4021 tacgcctttt ttcgcacctt gccaagcatt tttcaaaata ggggaatcaa aacgcctgat
     4081 ttatattatt gcttaaaatg ataaacaaac agaattaaat tttatatttt tgtatgattt
     4141 gatattaatt taaatattat gtggattttt agcataaaat atctgaatac ctttttaatg
     4201 aaaaaggctt atatgagatg cttgtttgca acattatgtt tttaaacaaa tttaattttg
     4261 attgaatttt aatatattaa ccggattttc cgcatcctcc cctttgtccg cagaataagc
     4321 ctctttttat tggcgtatcg ttaatcattt atttcaaaaa agttatttat tatcataata
     4381 tcaggaagcg aatcgttaaa aaaaactccc tcaaattcta tcaaattagt atttatccag
     4441 cacataaacg aaccaaagca ttctttcccc aagtgtaaat atttaaaatt aaactcaaaa
     4501 taagtttttt tgcttaaaaa atttaatggt ttttctatag tgaaaaaaaa gcccttactc
     4561 gttttagttc gggatactat tttaaataag tttttattcc caataaaata agaatattca
     4621 ttgactaagt tgttgtgatg aaaaagtaat tttattaaaa tgtctttata atcttccagg
     4681 ttcatttcta gctcgattta aaatgatttc attaaagcat tatttactac attttgtatg
     4741 accgggtacg catcagtctt taaaccggat tttgtttttg tcaatttaaa aaagcattgt
     4801 ttttgcaaac ggctgaagcg ttaacgccgc ccggaacatc ctgtttgcaa aaccagaatt
     4861 tcaaatttcc aaaaacaatc ggcggattcg ctctgaaacg cctgatttat tttgttactt
     4921 cgtgtaatcc gtaaaaatcg gggctgcctt ccggacgggt tttaaaacgc ttgtgcagcc
     4981 aaaaatactg ctcgggatgt tcgcgcacgc gttcctcgat aaaacggttc atgcgctgcg
     5041 cgtcggcttt cgcatcttca cccggaaagg atttccaagc cgggtagaat tgcagtgtaa
     5101 ccgtattgtc cgcctccctg acggggatgg cgggaatcac tttggcattt gcaagcgcgg
     5161 caatgcggct caagccggta atcgttgccg tccgaatacc gaagaaatcg acaaaaaccg
     5221 aatcgttgcg tccgaaatcc tgatcgggca gatacagaaa cggcgcgctg cttttgcgga
     5281 actgtttgac gagggcgcgc agcccttcgg tgcgcccgat aaggaaaacg ttgtgatagc
     5341 ggttgcggcc tttcaaaatc tgttcgtcca atatcttgtt tttttggtgg gaatacatac
     5401 tgatcagcgg aacatcctga ttaagcgcgt acaccgccat ctcgaacgcg gtaaagtgcg
     5461 gatacaggat gatgactttt tcccccgccg ccagcgcgtc gtccaaataa tgcttattgc
     5521 ggtaacgcac cagcgatttc aggcgtttag caggcgcata ccaatataag ccgtattcaa
     5581 gcatcagttt cgccatatgt ttgaaatgct gcttcaatac ggtttcgcgc ttttttccgt
     5641 cccactcggg aaagcatttt gccaaattga tttcgccgat acggcggcgg ggtttgacca
     5701 aaaggtaggc gagcaagcct gtcagatcgg caagtttgtg cagtagcgca aacggcagaa
     5761 actgcaaaac atacagtaca aaaaatataa atttcatctc gatacacatt ttcttttcag
     5821 acggcaaaat acaaatgccg tctgaaacta ttgaaacctg ccgcgcttga cctgcatccc
     5881 tgaaggattg agtttggcgg caagcccgtg gttgcgtaag gcgtgcgtca acgccaccgc
     5941 caaaccgtcc gccgcatccg cctgcggcgt tcccgaaagc cccagcatct gcaccaccat
     6001 atgctgcacc tgttctttcg ctgccttgcc cttgccgacc accgcctgtt tgacctgcaa
     6061 ggccgtgtat tccgaaacgg gcagcttatg gctgaccaat gccgccaatg ccgcgcccct
     6121 agcctgaccg agcatcagcg tcgatgcagg gttgacgttg acgaacacct gttccactgc
     6181 cgcctgttga ggcttgtaaa cggcaacgac ttcgccgatg tgccgcacaa tcacggcaat
     6241 cctgtctgcc agaggcgcat cggcaggcgt tttgatgcag ccggaggcga cgtaaaaatg
     6301 atcgcgcccc ctgacatcga tgatgccgaa acccgttacg cggctgcccg ggtcgatgcc
     6361 caagatacgg acgcttgcag ccatattcac aacaaaccgt gttgaatcag cttcttacgc
     6421 agggtattgc ggttcagccc cagcatcacg gaggctttgg actggttgcc gccgcattgt
     6481 tccatcacac acaccagcag cggtttttcc acctgatgca agaccatatc gtacacgccg
     6541 caaggttcgg taccgttcag gtctttgaaa tattgttcca aattttgtct gatacattgg
     6601 gaaatatcgg gaagggtatg gggcataatc gcgctttcaa aaggataatc gggtgttcag
     6661 acggcatttt ggcggtaggc gcacgcccaa atgtcggttt tcccggcaag tctttcaaga
     6721 tagcctgcaa gtgtgtcgta ttgcgccgcc gcactgtcca agcggttgat ttcacggcgc
     6781 gtctgctcgc cgtcgggcat ttcgtcgatg taccagccta tgtgtttgcg tgcgatgcgt
     6841 acgcctgcac tatcgccgta aaacgcgtgt atggcgcgga tgtggttcaa aatagcggcg
     6901 gcgcattctg ccaaactcaa ggcaggcggc aaaacaccgt gttcggcata atgtttcaaa
     6961 tcgcggaaga accacggcct gccttgcgcg ccgcgcccta tcataatgcc gtcggcggcg
     7021 gtttgtttga ggacggcttg ggctttttgc ggcgaagtaa tgtcgccgtt gacccagacc
     7081 gggatgttca gacggcattt ggtttcggcg atgagttcgt aacgcgcttc gcctttgtac
     7141 atttgcgtgc gcgtgcgtcc gtggacggca agggcggcga tgccgcaatc ttcggcgatt
     7201 ttggcgatga cgggcaggtt ttgatggtcg tcgtgccaac ccaaacgggt tttgagggta
     7261 acgggtacgc ctgccgcacg aacgacggct tccaaaatgg cggcaaccag cggctcgttc
     7321 tgcatcagcg cgctaccggc ttggacattg cagacttttt tagcgggaca gcccatgttg
     7381 atgtcgataa gctgcgcccc aaggctgacg ttgtaacgcg cggcatccgc catctgctgc
     7441 ggatcgcttc cggcaatctg cacggcaaca atgccgcctt catcggcaaa atcgctgcgg
     7501 tgcaaggttt ttctagtatt tctgagcgtc gggtcgctgg tcagcatttc gcacaccgcc
     7561 caacctgcgc caaaatctcg gcaaagtcgg cggaacggtt tgtcggtaat gcccgccatc
     7621 ggcgcaagtg cgatggggtt gtcgataaaa tagccgccga tgtgcataat ggatccgcgt
     7681 ttcaaaaaag tacgccattg tacatttttt aagcaggatt tccaatctcc ggacgcgccc
     7741 gcgattgggt cggacaccgt tttatggcat aatccgcaca cagattccct gccccgccac
     7801 tcacaggagg gcagtttata gtggatt
//
LOCUS       DAXHSF010000097         7770 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.097, whole genome shotgun sequence.
ACCESSION   DAXHSF010000097 DAXHSF010000000
VERSION     DAXHSF010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7770
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.097"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(19..1221)
                     /gene="trpB"
                     /locus_tag="ACFM8S_001420"
     CDS             complement(19..1221)
                     /gene="trpB"
                     /locus_tag="ACFM8S_001420"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFC6314485.1"
                     /translation="MKNYHAPDEKGFFGEHGGLYVSETLIPALQELADAYKAAKNDPE
                     FWEAFRHDLKHYVGRPSPVYHAARLSEHLGGAQIWLKREDLNHTGAHKVNNTIGQALL
                     AKRMGKKRVIAETGAGQHGVASATVAARFGMTCDVYMGADDIQRQMPNVFRMKLLGAN
                     VVGVESGSRTLKDAMNEAMREWVARVDDTFYIIGTAAGPTPYPEMVRDFQCVIGNEAK
                     AQMQEAIGRQPDVAVACVGGGSNAIGLFYPYIEEENVRLVGVEAGGLGVDTPDHAAPI
                     TSKAPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTVAK
                     DDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTV
                     AKLKGIKL"
     gene            complement(1406..2116)
                     /locus_tag="ACFM8S_001421"
     CDS             complement(1406..2116)
                     /locus_tag="ACFM8S_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HFC6314486.1"
                     /translation="MLFEGLPCLAVWCKNRLSAVLPVGGLNVSENLLEIETHPFDPVL
                     PSKAAVMMMGTFPPKEDKRAMQFHYPNFQNDMWRVYGLVFFGDADYFKVPSNGEAAHS
                     EKAFDAEKIKTFLHERGIASCPTVLKAVRQHGNASDKFLKVVETVDLAAVLAKIPDCR
                     HICTTGGKATEILLDIQGGGIKMPKTGETVPFPFAGRDLTLTRLPSTSRAYPLSLAKK
                     AAAYRAFFEMAGLCEKQL"
     gene            2328..3107
                     /gene="rsmA"
                     /locus_tag="ACFM8S_001422"
     CDS             2328..3107
                     /gene="rsmA"
                     /locus_tag="ACFM8S_001422"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFC6314487.1"
                     /translation="MKEHKARKRFGQNFLQDTRIISDIVNAVRPQADDVVIEIGPGLA
                     AITEPLAKKLNRLHVVEIDRDIVCRLKTLPFADKLVIHEGDVLQFDFNNIEGKKKIVG
                     NLPYNISTPLLFKLAEVADDVVDMHFMLQKEVVERMVAAPKSNDYGRLGVMLQYFFDM
                     EMLIDVPPESFDPAPKVDSAVVRMIPVKHRIGKADDFEHFAKLVKLAFHQRRKTIRNN
                     LKELAGDDDLQAVGINPQDRAEHIVPEKYVALSNYLAGKAV"
     gene            3280..4008
                     /locus_tag="ACFM8S_001423"
     CDS             3280..4008
                     /locus_tag="ACFM8S_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFC6314488.1"
                     /translation="MIKFKNVHKHFKDLHVINGVNLEIKKGEVVVVCGPSGSGKSTLI
                     RTVNQLESIESGEIWVDGVNVADPKTDLNKIREEVGFVFQGFNLYPHLTVLENITLAP
                     MKVKGQNAEQAEKKAMELLERVGLAHKKDAFPSQLSGGQQQRVAIARGLAMEPRVMLF
                     DEPTSALDPEMVGEVLKVMKDLAESGMTMMCVTHEMGFAREVADRVLFVDKGQILEDE
                     TPEAFFTNPKHERAKQFLQQVMTH"
     gene            4081..4440
                     /locus_tag="ACFM8S_001424"
     CDS             4081..4440
                     /locus_tag="ACFM8S_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314489.1"
                     /translation="MYFVDRTAVVLKPTVRFLEWLKSTDENMPDLTIEQLRANCSVFL
                     VPQFDEPEAVVSYFDERYRQIFEAELAGWDIDKDKWPQDMGLKAFWEFFDIEIHDMVL
                     DMEEAELNITPVFDNMM"
     gene            4445..4885
                     /locus_tag="ACFM8S_001425"
     CDS             4445..4885
                     /locus_tag="ACFM8S_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFC6314490.1"
                     /translation="MRAFQTALRPSRILNILTVSLHAASLAVCLTWFYGWMMWFGLAA
                     LVASYAYSLRITNLKHRHAITAITIDRDGQAEIISGKDKTAATLAGSSMVTPYALFLQ
                     WDTGGTTVSQCIIPDMTDKESYRRLKVWILWRQPKKTAETDTPS"
     gene            4917..6191
                     /gene="folC"
                     /locus_tag="ACFM8S_001426"
     CDS             4917..6191
                     /gene="folC"
                     /locus_tag="ACFM8S_001426"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFC6314491.1"
                     /translation="MKTLQDWLSHLETAHSGGLIDMGLERVGEVKKRMKLTPQCPVVV
                     VAGTNGKGSVCAYLTQIYKQAGFKVGTLTSPHLLRYNERIAVNAEPVSDDTIIASFER
                     IEAARGEISLTYFEFNALAAVDIFMREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSV
                     DLDHQAFLGDTVEQVGFEKAGVFRSGKPAICGQNPAPKSLLSHAEAIGAKLLMVQRDF
                     EFHALENIQWNYRFRPQHSDSPARNRNSLPFPALRGAYQLSNAACALTVLECLNDKLP
                     VDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALRRSLINLAFAQKRTAV
                     FSMLSDKDIDGVLEIVKDQFDEWYIAPLDMPRGMTLESLQNKLHEHSIENIQTFAAVR
                     EAYRAAASKAGENDRIVVFGSFHTVADVMSVL"
     gene            6204..7202
                     /gene="ftsN"
                     /locus_tag="ACFM8S_001427"
     CDS             6204..7202
                     /gene="ftsN"
                     /locus_tag="ACFM8S_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFC6314492.1"
                     /translation="MSENKQNEVLSGYEQLKRRNRRRLVTASCLVAASCILLAAALSS
                     GPAEQTAGETSGVENKAAGAAQTPALKSAADKPQDLAGEDKPSAADSEISEPENVGAP
                     LVLINERLEDSNIKGLEASEKLQQAETAKTAPKQAKQRAAEKVPATADSTDTVAVEKP
                     KRTAETKPQKAERTAKAKPKAKETKTAEKVADKPKTAAEKTKPDTAKSDSAVKEAKKA
                     DKAESKKTAEKDRSDGKKHETAQKTDKADKTKTAEKEKSGKKAAIQAGYAEKERALSL
                     QRKMKAAGIDSTITEIMTDNGKVYRVKSSNYKNARDAERDLNKLRVHGIAGQVTNE"
     gene            7195..7692
                     /locus_tag="ACFM8S_001428"
     CDS             7195..7692
                     /locus_tag="ACFM8S_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFC6314493.1"
                     /translation="MNSLPIADLLVSAVIAACIVISTMRGVITEAGSMAAWVVSFFFA
                     KLFAAPFADIAFASFQPRLFALALSFISLFVIACLIQKILRSLLTGAVSAVGLGFANR
                     ILGGVFGALKGILIITLLVMLASKTDLPDTEEWRQSYTLPFFVSLSEAVLNHSGGTAE
                     TPEDD"
BASE COUNT         1961 a         2176 c         2000 g         1633 t
ORIGIN      
        1 tatatcagac ggacgaggtt acagtttaat ccctttgagc ttcgcgacgg tattgatgtc
       61 tttgtcgcca cgaccggata ggttgaccag aatcacttgg tctttgccca ttttcggcgc
      121 gtttttcacc gcccaagcaa cggcgtggct ggattcgagc gcggggatga tgccctcgaa
      181 gcggcagagc aagtcaaagg cttcgagtgc ttcgtcgtct ttggcaacag tgtattcgac
      241 gcgcttgatg tcgtgcagat ggctgtgttc cgggccgatg ccggggtaat ccaagcctgc
      301 ggaaacagag tgtgtaccca agacttgacc gttttcgtcc tgcatcagat agctgcggaa
      361 accgtgcaat acgccgatag gggctttgct ggtaatcggt gcggcgtggt caggggtatc
      421 cacacccaaa ccgcctgctt ctacgccgac caaacgtacg ttttcttctt cgatataggg
      481 atagaacaaa ccgatggcgt tggagccgcc gcccacgcag gcaacggcaa cgtcgggctg
      541 tctgccgatg gcttcctgca tctgcgcttt agcttcgttg ccgatcacgc attggaaatc
      601 gcgcaccatt tcgggatacg gcgtggggcc ggcggcggta ccgatgatgt agaatgtgtc
      661 atccacgcgg gcgacccatt cgcgcatggc ttcgttcatc gcgtctttca gcgtgcggct
      721 gccgctttct acaccgacca cgttcgcacc caataatttc atacggaaca cgttgggcat
      781 ttggcgttgg atgtcgtccg cgcccatata cacgtcgcaa gtcataccga agcgtgcggc
      841 aacggtggca ctcgccacgc cgtgctgacc cgcgccggtt tcggcgatga cgcgtttttt
      901 acccatacgt tttgccaaca gtgcctgacc gatggtgttg ttgactttgt gcgcgccggt
      961 gtggttcaag tcttcgcgct tcaaccaaat ttgcgcgcca cccaaatgct cggacaatcg
     1021 cgcggcatgg taaacggggc tgggcctgcc gacataatgt ttcaaatcat ggcggaacgc
     1081 ttcccaaaat tcaggatcgt ttttcgctgc cttataggca tccgccagct cttgcaaggc
     1141 gggaatcagg gtttcggaga cataaagccc gccgtgttcg ccgaaaaagc ccttctcgtc
     1201 gggcgcgtgg tagttcttca tcagatttcg tccttgtgct tcaaattttc agacggcaat
     1261 ttatagtgga ttaacaaaaa ccggtacggc gttgccccgc cttgccgtac tatttgtact
     1321 gtctgcggct tcgccgcctt gtcctgattt ttgttaatcc actatatttt tcaaacatgc
     1381 cgtccgaacg ggaaattgtc ggcaattata actgtttttc acacaaaccc gccatttcga
     1441 aaaacgcccg atacgccgcc gcctttttcg ccaaactcaa aggataggca cgcgaagtcg
     1501 aaggcaggcg cgtcagggtc aaatcccgtc cggcaaacgg aaacggcacg gtttcgcccg
     1561 ttttcggcat tttgatgccg ccaccctgaa tatcgagcag gatttccgtc gccttgccgc
     1621 ctgtcgtaca aatatggcgg cagtctggta ttttcgccaa caccgccgcc aaatcgacgg
     1681 tttcgactac ctttaaaaac ttgtcggacg cattgccgtg ctgacgtacc gccttcaaca
     1741 cggtcgggca ggacgcaatc ccccgttcgt gcaaaaacgt tttgattttc tccgcgtcaa
     1801 acgctttttc agagtgcgca gcctccccgt tggacggcac tttgaaataa tccgcatcgc
     1861 cgaaaaacac cagcccgtaa acgcgccaca tatcgttttg gaaattcgga taatgaaact
     1921 gcatcgcgcg tttgtcttcc ttgggcggaa acgtccccat catcatgacc gcagccttcg
     1981 acggcaacac gggatcgaag ggatgggttt cgatttcaag cagattttca gacacgttca
     2041 atcctccaac aggcaaaacg gcagacagcc ggtttttgca ccaaaccgcc aaacacggta
     2101 agccttcaaa cagcattatc acgccctgcc gttttcggtt acaatgccga catcaaacgg
     2161 atactgtaaa cacatttatg tttcaacaca caggacgaca cataaagcac cgccctatgt
     2221 gccgtcctga tttggaagga gttacgcccc tcccaaatag agtctgattc taccgccccg
     2281 aaggacagat gtccaagtgg cggggtttca accgaaaagg aaacacgatg aaagaacaca
     2341 aagcccgcaa gcgtttcggg cagaattttt tgcaggacac gcggattatc agcgatattg
     2401 tcaacgccgt gcgcccgcag gcggatgatg tcgtgattga aatcggcccg ggtttggcgg
     2461 cgattaccga acctttggcg aaaaagctga accgcctgca cgttgtcgaa atcgaccgcg
     2521 acatcgtatg ccgtctgaaa acgctgccgt ttgcggataa actcgtcatt catgaaggcg
     2581 acgtgttgca gtttgatttc aacaacattg aaggcaaaaa gaaaatcgtc ggcaacctgc
     2641 cgtacaacat ttccacgccg cttttgttca agctggcgga ggtggcggac gatgtcgtcg
     2701 atatgcactt tatgctgcaa aaagaagtgg tcgagcgtat ggttgccgcg ccgaaaagca
     2761 acgactacgg gcgtttgggc gtgatgctgc aatatttttt cgatatggaa atgctgattg
     2821 acgtaccgcc cgaatcgttc gaccctgcgc cgaaagtgga ttccgccgtc gtccgtatga
     2881 ttccggtgaa acaccgcatc ggcaaggcgg acgatttcga gcatttcgcc aaactggtga
     2941 aactcgcctt ccaccagcgg cgcaaaacca tacgcaacaa tctgaaagag cttgcaggcg
     3001 acgatgattt gcaggcagtc ggcatcaatc cgcaagaccg cgccgaacac atcgtgccgg
     3061 agaaatacgt ggcgttgagc aattatctgg cgggcaaggc cgtctgaaaa gggagtaagc
     3121 cgatgacgaa atacctgctt ttacccctga tactgccgct tgcagcctgc ctgaccaatc
     3181 cgaacgaccg tttttttgac ggcagacgct acacgcttca tcatatggag ctcccgtaac
     3241 gacaacctgc aacggcaaat gccgtctgaa agaaaagaca tgatcaaatt caaaaacgta
     3301 cacaaacatt tcaaagacct gcacgtcatc aacggcgtaa acttggaaat caaaaaaggc
     3361 gaagtggtcg tcgtctgcgg gccttcgggc agcggcaaat ccacactcat ccgcactgtc
     3421 aaccagcttg aaagcatcga aagcggcgag atttgggtgg acggtgtcaa cgttgccgac
     3481 cccaaaaccg atttgaacaa aatccgcgaa gaagtcggct tcgtattcca aggcttcaac
     3541 ctctacccgc acctgaccgt gttggaaaac attaccctcg caccgatgaa ggttaagggg
     3601 caaaatgcgg aacaggcaga gaaaaaagcg atggaacttt tggaacgcgt cggactggca
     3661 cacaaaaaag acgccttccc ttcccaactt tccggcggcc agcagcaacg cgtggcgatt
     3721 gcacgcggtt tggcgatgga accgcgcgta atgttgtttg acgaaccgac ctccgcactc
     3781 gaccccgaaa tggtcggcga agtgttgaaa gtgatgaaag acttggcgga aagcggtatg
     3841 acgatgatgt gcgtaaccca cgaaatgggt tttgcccgcg aagttgccga ccgagtgctt
     3901 tttgtcgata aagggcaaat cctcgaagac gaaacgccgg aagcgttttt taccaacccg
     3961 aaacacgaac gcgccaaaca attcctgcaa caggtcatga cccactgatg ctatgccgtc
     4021 tgaaaaccga acgttcagac ggcatagccc gacaccgcaa gatgattgag aaagaaaacc
     4081 atgtattttg ttgaccgcac cgccgtcgtc ctcaagccga ccgtccgctt tttggaatgg
     4141 ctcaaaagca ccgacgaaaa tatgcccgac ctgaccatag aacagcttcg tgccaactgt
     4201 tccgtcttcc tcgttccgca gttcgacgaa ccggaagccg tcgtttccta tttcgacgaa
     4261 cgctaccgcc agattttcga ggcggagctg gcaggttggg acatcgataa ggataaatgg
     4321 ccgcaagata tggggctgaa ggcgttttgg gaatttttcg acatcgaaat ccacgatatg
     4381 gtgttggaca tggaagaagc ggaattgaac attacccccg tgttcgacaa catgatgtaa
     4441 ggctgtgcgc gcctttcaga cggcattgag gccgtcccgc atcctgaaca tcctgactgt
     4501 atcgcttcac gccgcctccc ttgccgtctg cctgacttgg ttttacggct ggatgatgtg
     4561 gttcggtttg gcggctttgg tggcaagtta cgcatactcg ctcaggataa cgaatctgaa
     4621 acaccgccat gccataaccg ccattaccat agaccgcgac ggacaggcgg aaatcatatc
     4681 cggcaaagac aaaacagcgg caacacttgc gggcagcagt atggttacgc cttatgcctt
     4741 attcttacaa tgggacacag gcggcacaac cgtcagccaa tgcatcatac ccgacatgac
     4801 ggacaaagaa tcctaccggc ggctgaaagt ctggatactt tggcggcagc cgaaaaaaac
     4861 cgccgaaacc gatacaccaa gctgatttca gacggcattt gaacacggaa cactcaatga
     4921 aaacactgca agactggctc tcgcatttgg aaaccgccca cagcggtggc ttgatcgata
     4981 tgggcttgga acgcgtaggc gaagtgaaaa aacggatgaa gctcacgccg caatgccccg
     5041 tcgtcgttgt cgcgggaaca aacggcaaag gttcggtctg cgcctacctg acacagattt
     5101 acaaacaggc aggctttaaa gtcggtacgc tgaccagccc gcatttattg cgttacaacg
     5161 aacgcatcgc cgtcaacgcc gaacccgttt cggacgatac gattattgcc tctttcgagc
     5221 gcatcgaagc ggcgcgcggc gaaatatcgc tgacttattt tgaattcaat gccttggcgg
     5281 cggtcgacat cttcatgcgc gaacaggttg acgtgatgat attggaagtc ggcttgggcg
     5341 gacgcttgga cgcggtcaac gcgtttgacg gcgattgcgc ggtggttacc agcgtggatt
     5401 tggaccatca ggcatttttg ggcgatacgg tcgagcaggt cggctttgaa aaagcaggcg
     5461 tgttccgcag cggcaaaccc gccatctgcg gtcaaaaccc cgcgcccaaa tcgttgcttt
     5521 cacacgccga agccataggc gcgaaactgc tgatggtgca gcgcgatttc gagtttcacg
     5581 cgctggaaaa catccaatgg aactaccgct tccgaccgca gcattcagac agccccgctc
     5641 gcaaccgtaa ttccctgccc ttccccgcat tgcgcggcgc ataccaactt tccaatgccg
     5701 cctgtgcgct gaccgtgttg gaatgtttga acgacaaatt gccggtggac atcggtgcaa
     5761 tcaaacgcgg attgctgctg gttgaaaatc ccggacgctt ccaagtcctg cccggccggc
     5821 cgctgaccgt tttggacgtc ggacacaatc cccacgctgc ccgcgccctg cgccgcagcc
     5881 tgattaattt ggcgtttgcg caaaaacgca ccgccgtgtt cagtatgctg tccgacaaag
     5941 acatagacgg cgtgttggaa attgtcaaag accagtttga cgaatggtat atcgcacctt
     6001 tggacatgcc gcgcggcatg acgctcgaga gcctgcaaaa caaactgcac gaacacagca
     6061 tcgaaaacat acagacattc gccgccgtcc gcgaagcata ccgggcggca gcctcaaaag
     6121 cgggcgagaa cgacagaatc gtcgttttcg gctcatttca taccgttgcc gatgtcatgt
     6181 cggtacttta aaaggaaact tctatgtccg aaaacaaaca aaacgaagtc ctgagcggtt
     6241 acgaacaact caaacggcgc aaccgccgcc gcctcgtaac ggcaagttgc ttggttgccg
     6301 cctcctgcat cctgctggca gccgccctca gttccggccc tgccgaacag actgccggcg
     6361 aaacaagcgg cgtagaaaac aaagcggcag gtgcggcaca aacccctgcc ttgaaatccg
     6421 ccgccgacaa accgcaggac ttggcaggcg aagacaagcc ttctgccgcc gacagcgaaa
     6481 tcagcgagcc tgaaaacgta ggcgcgccgc tggtgctgat taacgagcgc ctcgaagaca
     6541 gcaacatcaa aggtttggaa gcatccgaga aactgcaaca ggcagaaacc gccaaaaccg
     6601 caccgaagca ggcaaaacaa cgcgctgccg aaaaagtgcc ggcaactgcc gacagtacgg
     6661 atacggtagc ggttgaaaaa ccgaaacgca ctgccgaaac aaaaccgcaa aaagcggaac
     6721 gcactgccaa agccaagccc aaagccaaag aaaccaaaac cgccgaaaaa gttgccgaca
     6781 aaccgaaaac tgccgccgaa aaaaccaaac cggatacggc aaaatccgac agcgcggtaa
     6841 aagaagcgaa aaaagccgac aaggctgaaa gcaaaaaaac agccgaaaaa gaccgttcgg
     6901 acggcaaaaa acacgaaacg gcacaaaaaa ccgacaaagc ggacaagacc aaaaccgccg
     6961 agaaggaaaa atccggtaaa aaagccgcca ttcaggcagg ttatgccgaa aaagaacgcg
     7021 ccttaagcct ccagcgcaaa atgaaggcgg cgggtatcga ttcgaccatc accgaaatta
     7081 tgaccgacaa cggcaaagtt taccgcgtca aatcaagcaa ctataaaaac gcaagggatg
     7141 ccgaacgcga tttgaacaaa ttgcgcgtac acggtatcgc cggtcaggta acgaatgaat
     7201 agcctcccca ttgccgacct cctcgtctcc gccgtcatcg ccgcctgcat cgtcatttcc
     7261 acgatgcgcg gcgtgattac ggaggcaggc tcaatggcgg catgggtggt ttccttcttt
     7321 ttcgccaaac tctttgccgc acccttcgcc gacatcgcct ttgcatcgtt ccaaccccgc
     7381 ctgtttgcat tggctctgtc gttcatttcc ctattcgtca ttgcctgtct gatccagaaa
     7441 atactccgct cgctgctgac cggggcagtt tcggcggtcg gtttgggctt tgccaaccgc
     7501 attttgggcg gcgtattcgg tgcattgaaa ggcattttga ttattaccct gctggtcatg
     7561 ctcgcttcaa aaaccgacct gcccgatacc gaagaatggc ggcaatctta cacactgccg
     7621 tttttcgtat cgctttccga agccgtgttg aaccatagcg gcggcacggc ggaaactccg
     7681 gaagacgatt gattgccata cggatatttc agacggcatt ctcttttatg ccgtctgaaa
     7741 tgttttttcc tttcctcagc catatcacag
//
LOCUS       DAXHSF010000098         7724 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.098, whole genome shotgun sequence.
ACCESSION   DAXHSF010000098 DAXHSF010000000
VERSION     DAXHSF010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7724
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.098"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(14..430)
                     /gene="dksA"
                     /locus_tag="ACFM8S_001429"
     CDS             complement(14..430)
                     /gene="dksA"
                     /locus_tag="ACFM8S_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFC6314494.1"
                     /translation="MAKLTEQDILNWSGPEDDYMNDDHLAFFRELLVKMQDELIENAS
                     ATTGHLQEHESAPDPADRATQEEEYALELRTRDRERKLLSKIQATIRNIDEGDYGFCA
                     DTGEPIGLKRLLARPTATLSVESQERRERMKKQFAD"
     gene            complement(577..1386)
                     /gene="proC"
                     /locus_tag="ACFM8S_001430"
     CDS             complement(577..1386)
                     /gene="proC"
                     /locus_tag="ACFM8S_001430"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFC6314495.1"
                     /translation="MNRVEIMNVYFLGGGNMAAAVAGGLVKQGGYRIHIANRGAEKRE
                     RLQKELGVETSETLPELHSDDVLILAVKPQDMEAACKNIRTNGALVLSVAAGLPVDTL
                     SRYIGGTRRIVRVMPNTPGKIGLGVSGMFAEAEVSETDRRIADRIMKSVGLTVWLDDE
                     EKMHGITGISGSGPAYVFYLLDALQNAAIRQGFDMAEARALSLATFKGAVALAEQTGE
                     DFEKLQKNVTSKGGTTHEAVEAFRRHRVAEAISEGVCACVRRSQEMERQYQ"
     gene            complement(1459..1824)
                     /locus_tag="ACFM8S_001431"
     CDS             complement(1459..1824)
                     /locus_tag="ACFM8S_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFC6314496.1"
                     /translation="MEIRAIKYTAMAALLAFTVAGCRLAGWYECSSLSGWCKPRKPAA
                     IDFWDIGGESPPSLEDYEIPLSDGNRSVRANEYESAQQSYFYRKIRKFEACGLDWRTR
                     DGKPLIETFKQEGFDCLKK"
     gene            complement(1824..2510)
                     /locus_tag="ACFM8S_001432"
     CDS             complement(1824..2510)
                     /locus_tag="ACFM8S_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFC6314497.1"
                     /translation="MTVLQERYCEVSDRIGKLVLQAGRKPHSVSLIAVGKTFPSDGIR
                     EVYAAGQRDFGENYIQEWYGKTEELADLTDIVWHVIGDVQSNKTKFVAERAHWVHTVC
                     RLKTAVRLSGQRPSSMPPLQVCIEVNIAGEAVKHGVAPEEAVALAVEVAKLPNIVVRG
                     LMCVAKANSSETELKVQFQTMRKLLADLNTAGVKADVLSMGMSDDMPAAIECGATHVR
                     IGSAIFGKRG"
     gene            2630..3673
                     /gene="pilT"
                     /locus_tag="ACFM8S_001433"
     CDS             2630..3673
                     /gene="pilT"
                     /locus_tag="ACFM8S_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFC6314498.1"
                     /translation="MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSA
                     EEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVARFRVNAFNIGRGPAAVFRTIPST
                     VLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
                     PIEFVHQSKKSLINQRELHQHTLSFANALRSALREDPDVILVGEMRDPETIGLALTAA
                     ETGHLVFGTLHTTGAAKTVDRIVDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNG
                     RVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEV
                     ARRRAQNSESMSF"
     gene            3836..5062
                     /locus_tag="ACFM8S_001434"
     CDS             3836..5062
                     /locus_tag="ACFM8S_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HFC6314499.1"
                     /translation="MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCE
                     TAEAQNASDILISKGFPPSLKINSALTPQPQKALTGEETAAIAASTMNAEQSEIFRRD
                     GEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
                     LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDT
                     INWQTAVQNAMRQSPDVVCIGEVRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERI
                     LNFYPKEQHKQILIDIALNLTGIICQRLALKQDKTGRTAVVDLLINTPAIQDFILKGD
                     LMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGK
                     MPELLYDRVNGLNLIS"
     gene            5348..7498
                     /gene="yccS"
                     /locus_tag="ACFM8S_001435"
     CDS             5348..7498
                     /gene="yccS"
                     /locus_tag="ACFM8S_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFC6314500.1"
                     /translation="MKTPLLKPLLITSLPVFASVFTAASIVWQLGEPKLAMPFVLGII
                     AGGLVDLDNRLTGRLKNIIATVALFTLSSLTAQSTLGTGLPFILAMTLMTFGFTILGA
                     VGLKYRTFAFGALAVATYTTLTYTPETYWLTNPFMILCGTVLYSTAIILFQIILPHRP
                     VQESVANAYDALGGYLEAKADFFDPDEAAWIGNRHIDLAMSNTGVITAFNQCRSALFY
                     RLRGKHRHPRTAKMLRYYFAAQDIHERISSAHVDYQEMSEKFKNTDIIFRIHRLLEMQ
                     GQACRNTAQALRASKDYVYSKRLGRAIEGCRQSLRLLSDSNDSPDIRHLRRLLDNLGS
                     VDQQFRQLQHNGLQAENDRMGDTRIAALETSSLKNTWQAIRPQLNLESGVFRHAVRLS
                     LVVAAACTIVEALNLNLGYWILLTALFVCQPNYTATKSRVRQRIAGTVLGVIVGSLVP
                     YFTPSVETKLWIVVVSTTLFFMTRTYKYSFSTFFITIQALTSLSLAGLDVYAAMPVRI
                     IDTIIGASLAWAAVSYLWPDWKYLTLERTAALAVRSSGAYLEKITERLKSGETGDDVE
                     YRATRRRAHEHTAALSSTLSDMSSEPAKFADSLQPGFTLLKTGYALTGYISALGAYRS
                     EMHEECSPDFTSQFHLAAEHTAHIFQHLPETEPDDFQTALDTLRGELDTLRTHSSGTQ
                     SHILLQQLQLIARQLEPYYRAYRQIPHRQPQNAA"
BASE COUNT         1894 a         2557 c         1696 g         1577 t
ORIGIN      
        1 ttgtttgccg ccatcagtcg gcaaactgtt ttttcatcct ctctcggcgt tcttgggact
       61 caacagataa agtggctgtc gggcgtgcca gcagccgctt cagaccgata ggctctcccg
      121 tatcggcaca gaatccataa tccccttcat caatattgcg gatggtcgcc tgtattttac
      181 tgagaagttt ccgttcccga tcgcgggtac ggagttccaa tgcgtactct tcttcctgtg
      241 tggcacggtc ggcaggatcg ggggctgatt cgtgttcttg gagatgccct gtcgtagcgg
      301 aagcattttc gatgagttcg tcttgcattt ttaccagcaa ttcgcggaaa aaagccaaat
      361 ggtcgtcatt catataatcg tcttccggcc cgctccaatt caaaatatct tgttctgtca
      421 gctttgccat aatgtatatc ctctcggttc accgcaaaaa acaaagcacc acaaagtggc
      481 tttattcctt tgatttttat aaggcttatt gcctaaggcg tttttgaaaa cgcgcatcat
      541 aacacgtttt ggattggttt ttttattttc tttacattat tgatattgcc gttccatttc
      601 ctgcgaacgg cgcacacagg cacaaacgcc ctcgcttatg gcttcggcga cacgatgccg
      661 cctgaaagct tccacggctt cgtgggttgt cccgcctttt gacgttacat ttttttgaag
      721 cttctcgaaa tcttcacccg tctgctcggc aagggcaacc gctcctttaa acgttgccag
      781 actgagcgcg cgtgcttctg ccatatcaaa cccttgtcgg atggcggcat tttgcaatgc
      841 gtccagcaga taaaacacat aagccggtcc gctgccgctg ataccggtaa tgccgtgcat
      901 tttttcctca tcatccaacc aaacagtcaa accgactgat ttcatgattc gatcggcaat
      961 cctgcggtct gtttccgata cttccgcttc ggcaaacata ccggatacgc ccagcccgat
     1021 ttttccgggt gtattcggca taacccggac aatgcggcgt gttcccccaa tgtaacggct
     1081 gagcgtatcg accggcaatc cggctgcgac agaaagcacc aatgcgccgt tggtgcggat
     1141 atttttgcac gcggcttcca tatcctgcgg cttgacggca aggattaaaa catcgtcgga
     1201 atgaagctcc ggcaaggttt cagaagtttc gacccccaac tctttttgca aacgttcgcg
     1261 cttttccgca ccccgattgg ctatatggat gcggtaaccg ccttgtttga ccaatccgcc
     1321 cgcaacggca gccgccatat tgccgccgcc gagaaaataa acattcatta tttctaccct
     1381 attcaaaatt acctgactaa attcattccc aattttttac catcggacgc gctcggacag
     1441 accgttgcgc cgcaacccct actttttcaa acaatcaaaa ccttcctgtt tgaacgtctc
     1501 aatcaaaggt ttgccgtcac gcgtacgcca atccaacccg caggcttcaa acttccttat
     1561 tttcctgtaa aagtaagatt gttgtgcgga ttcatattca tttgccctga cggaacgatt
     1621 gccgtctgaa agcggtatct cgtagtcctc taaagacggc ggactctcgc cgccaatatc
     1681 ccaaaaatcg atggcggcag gttttctcgg cttacaccag ccggacaggg acgaacactc
     1741 ataccaacct gccaaccggc agcctgcaac cgtaaatgca agcaacgcag ccattgccgt
     1801 atattttatt gcccgaattt ccatcagccc cttttcccga aaatcgcgct gccgatacgg
     1861 acgtgtgtcg caccgcactc aatggcggca ggcatatcgt ccgacatccc catagacagc
     1921 acgtcagcct taacgccagc cgtattgagg tcggcaagca gtttccgcat cgtttgaaat
     1981 tgcaccttca actccgtttc actgctgttg gctttggcaa cacacatcag tccgcgtacg
     2041 acgatattcg gcagcttcgc cacttccaca gcaagcgcga ctgcttcttc gggcgcgaca
     2101 ccgtgcttca ccgcctcgcc cgcaatgttc acctcaatac acacctgcaa aggcggcatt
     2161 gaggaaggac gttgcccgct cagccggaca gcggtcttta gacggcatac ggtatgcacc
     2221 caatgcgcgc gttcggcgac aaacttggtt ttgttggact gcacatcgcc gatgacgtgc
     2281 cacacgatgt cggtcaaatc cgccaactct tccgttttgc cgtaccactc ctgaatatag
     2341 ttctcgccga aatcacgctg tccggcggcg taaacttcgc ggatgccgtc tgaagggaaa
     2401 gtcttaccga cggcaatcag gctgacggaa tgcggcttcc ttcccgcctg cagaaccaat
     2461 tttccaatac ggtcggacac ctcacaataa cgttcttgca acaccgtcat agattatccc
     2521 ctaattaaaa atgattaaac agttgaaacc cctccagtca gggcggtaca atcaaggttg
     2581 ttagaaccat tccaaccaat cgaaacatta tactaaacag agccgcatta tgcagattac
     2641 cgacttactc gccttcggcg ctaaaaacaa agcatccgac cttcacctga gttcgggcat
     2701 atcccctatg attcgggttc acggcgatat gcggcgcatc aaccttcccg aaatgagcgc
     2761 ggaagaggtc ggtaatatgg taacttcggt gatgaacgac caccagcgga aaatctacca
     2821 gcaaaacttg gaagttgact tctcgttcga actgcccaac gtcgcccgat tccgcgtcaa
     2881 cgccttcaac atcggccgcg gtcccgccgc cgtattccgc accattccca gcaccgtctt
     2941 atcgctggaa gaattgaaag ccccgagcat tttccaaaaa atcgcagaat cgccgcgcgg
     3001 catggtattg gttaccggcc ctaccggttc gggcaaatcg accacgcttg ccgcgatgat
     3061 caactacatc aacgaaaccc agccggcaca catcctgacc atcgaagacc cgatcgaatt
     3121 cgtccaccaa agcaaaaaat ccctgattaa ccagcgcgag ctgcaccagc acaccctcag
     3181 cttcgccaac gcgctgcgtt ccgcattgcg cgaagacccc gacgttatcc ttgtcggcga
     3241 gatgcgcgac cccgaaacca tcggcttggc actgaccgcc gccgaaaccg gacacttggt
     3301 tttcggcacg ctgcacacga ccggcgcagc aaaaaccgtc gaccgtatcg tggacgtatt
     3361 cccggcggga gaaaaagaaa tggtgcgctc tatgctgtcc gaatcgctga ccgccgtcat
     3421 ctcccaaaac ctgctgaaaa cgcacgacgg caacggccgt gtcgcctcgc acgaaatcct
     3481 gattgccaac cccgccgtcc gcaacctcat ccgcgaaaac aaaatcacgc agattaactc
     3541 cgtcctgcaa accgggcagg cgagcggtat gcagacaatg gaccaatcgc tgcaatcgct
     3601 ggtgcgccaa gggctgatcg caccggaagt cgcacgcaga cgcgcgcaaa acagcgaaag
     3661 tatgagtttc tgacacacaa ccgccttccg gccataccgg cgggaaaaca aggcgcaaac
     3721 acgcggggcg ggacgcagca tcccgcccgg ctacctttcc gaacaagaag cgtccgcctt
     3781 cctgttgaaa cctgccgccg caaactgcaa ggcttaaacc gaaaagaagc taacgatgaa
     3841 taccgataac ctgcacgaca tcttggacga aatggttcaa gtgtattctc aaaaaaaaca
     3901 aagccgatcc gaaaccccgg ccgaaatcgg cgcacacttc cacccgctgc tcgaccgcct
     3961 gtgcgaaacc gcagaagcac aaaacgcgtc cgacatcctt atcagcaaag gattcccgcc
     4021 ctcgttgaaa atcaacagcg cattaacccc gcagccgcaa aaggcgctga cgggcgagga
     4081 aaccgccgcc atcgccgcat cgacgatgaa cgccgaacaa tcggaaatat tccggcgcga
     4141 cggcgaaatc aactactccg tccagtcgcg cagcggcacg cgctaccgcg ccaacgccta
     4201 ccacagccaa ggcagcgcag gtttggtttt gcggcgcatc aaccacgtca tcccgcaaat
     4261 gcaggaatta ggcctgcccg aaaaactcaa agacctcgcc gtcgcaccgc gcgggctgct
     4321 gattatcgtc gggcctaccg gttcgggcaa atccaccacg atggcgacta tgctcgaaca
     4381 ccgcaacaaa accctgccca gccatatcgt taccatcgaa gacccgattg agtttatcta
     4441 caaaccgcgc cgctgcatct ttacccagcg cgaaatcggc gtcgacacca taaactggca
     4501 gacggcggta caaaacgcta tgcgccaatc ccccgacgtg gtctgcatcg gcgaagtccg
     4561 cagcagggaa agtatggaat acgcgatgca gctcgcccaa accggccacc tgtgcatttt
     4621 tacgctccac gccaacaccg cgccgcagtc gctcgaacgc atactcaact tctaccccaa
     4681 agaacagcac aagcaaatac tgatcgacat cgccctcaac ctgaccggca tcatctgcca
     4741 acgcctcgcc ctcaaacaag acaaaacggg caggacggcg gttgtcgact tgctcatcaa
     4801 cacgcccgcc atccaagact tcatcctgaa gggcgacctg atgaacatca gtaaaatcat
     4861 ggaaaccgcc aaaaccgacg gaatgcagac gatggatcaa aaccttttcg aactgtaccg
     4921 tcacggcatc atcagttacg aagaagccct gcgccagtcc gtttccgcca acaacctgcg
     4981 attgcacatc caactgcaca aagaaggcaa aatgcccgaa ctcctttacg acagggtcaa
     5041 cggtctcaac ctcatttcct gatccgcaaa acccaatgcc gtctgaaaac cgcatccccg
     5101 ttttcagacg gcatgatttt atccgcccca ttcatgtgct acactttata gtggattaaa
     5161 tttatagtgg attaacaaaa atcaggacaa gacgacgaag ccgcagacag cacaaatagt
     5221 acggaaccga ttcacttggt gcttcagcac cttagagaat cgttctcttt cagctaaggc
     5281 gaggcaacgc cgtactggtt tttgttaatc cactatattc acttcataac aataaaccgg
     5341 taaaaccatg aaaaccccac tcctcaagcc tctgctcatt acctcgcttc ccgttttcgc
     5401 cagtgttttt accgccgcct ccatcgtctg gcagctaggc gaacccaagc tcgccatgcc
     5461 cttcgtactc ggcatcatcg ccggcggcct tgtcgatttg gacaaccgcc tgaccggacg
     5521 gctgaaaaac atcatcgcca ccgtcgccct gttcaccctc tcctcgctca cggcacaaag
     5581 taccctcggc acagggctgc ccttcatcct cgccatgacc ctgatgacct tcggctttac
     5641 cattttaggc gcggtcgggc tgaaataccg caccttcgcc ttcggcgcac tcgccgtcgc
     5701 cacctacacc acacttacct acacccccga aacctactgg ctgaccaacc ccttcatgat
     5761 tttatgcggc accgtactgt acagcaccgc catcatcctg ttccaaatca tcctgcccca
     5821 ccgccccgtc caagaaagcg tcgccaacgc ctacgacgca ctcggcggct acctcgaagc
     5881 caaagccgac ttcttcgacc ccgatgaggc agcctggata ggcaaccgcc acatcgacct
     5941 cgccatgagc aacaccggcg tcatcaccgc cttcaaccaa tgccgttccg ccctgtttta
     6001 ccgtttgcgc ggcaaacacc gccacccgcg caccgccaaa atgctgcgct actacttcgc
     6061 cgcccaagac atccacgaac gcatcagctc cgcccacgtc gattatcagg aaatgtccga
     6121 aaaattcaaa aacaccgaca tcatcttccg catccaccgc ctgctcgaaa tgcagggaca
     6181 agcctgccgc aacaccgccc aagccctgcg cgcaagcaaa gactacgttt acagcaaacg
     6241 cctcggccgc gccatcgaag gctgccgcca atcgctgcgc ctcctttcag acagcaacga
     6301 cagtcccgac atccgccacc tgcgccgcct tctcgacaac ctcggcagcg tcgaccagca
     6361 gttccgccaa ctccagcaca acggcctgca ggcagaaaac gaccgcatgg gcgacacccg
     6421 catcgccgcc ctcgaaacca gcagcctcaa aaacacctgg caggcaatcc gtccgcagct
     6481 aaacctcgaa tcaggcgtat tccgccatgc cgtccgcctg tccctcgtcg ttgccgccgc
     6541 ctgcaccatc gtcgaagccc tcaacctcaa cctcggctac tggatactac tgaccgccct
     6601 tttcgtctgc caacccaact acaccgccac caaaagccgc gtccgccagc gcatcgccgg
     6661 taccgtactc ggcgtaatcg tcggctcgct cgtcccctac ttcaccccgt ctgtcgaaac
     6721 caagctttgg atcgtcgtcg tcagcaccac cctgttcttc atgacccgca cctacaaata
     6781 cagcttctcg acatttttca tcaccattca ggcactgacc agcctgtcgc ttgcagggtt
     6841 ggacgtatat gccgccatgc ccgtgcgcat catcgacacc attatcggcg catcccttgc
     6901 ctgggcggcg gtcagctacc tgtggcccga ctggaaatac ctcacgctcg aacgcaccgc
     6961 cgcccttgcc gtacgcagca gcggcgccta tctcgaaaaa atcaccgaac gcctcaaaag
     7021 cggcgaaacc ggcgacgacg tcgaataccg cgccacccgc cgccgcgccc acgaacacac
     7081 cgccgccctc agcagcaccc tttccgacat gagcagcgaa cccgcaaaat tcgccgacag
     7141 cctgcaaccc ggctttaccc tgctcaaaac cggctacgcc ctgaccggct acatctccgc
     7201 cctcggcgca taccgtagcg aaatgcatga agaatgcagc cccgacttta cctcacagtt
     7261 ccacctcgcc gccgaacaca ccgcccacat cttccaacac ctgcccgaaa ccgaacccga
     7321 cgactttcag acagcactgg atacactgcg cggcgaactc gacaccctcc gcacccacag
     7381 cagcggaaca caaagccaca tcctcctcca acagctccaa ctcatcgccc ggcagctcga
     7441 accctactac cgcgcctacc gacaaattcc gcacaggcag ccccaaaacg cagcctgaaa
     7501 aagtttcggc attttgtaag agagggcaga ttgtcagaca ggttgcaaga tagtggataa
     7561 agctttgcgc agggtaaatg cgtagcaact gaaccgtcat tcccacgaaa gtgggaatcc
     7621 agaatctcgg actttcagat aatctttgaa tattactgtt gttctaaggt ctggattccc
     7681 gcctgcgcgg gaatgacgaa tccatccgta cggaaacctg cacc
//
LOCUS       DAXHSF010000099         7566 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.099, whole genome shotgun sequence.
ACCESSION   DAXHSF010000099 DAXHSF010000000
VERSION     DAXHSF010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7566
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.099"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            1..347
                     /locus_tag="ACFM8S_001436"
                     /pseudo
     CDS             1..347
                     /locus_tag="ACFM8S_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153708080.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(546..686)
                     /locus_tag="ACFM8S_001437"
     CDS             complement(546..686)
                     /locus_tag="ACFM8S_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314501.1"
                     /translation="MPEYRNWQNGLNAKCADGDEYGVAQSDCRTREINAKTKEIQGYL
                     ID"
     gene            complement(731..1153)
                     /locus_tag="ACFM8S_001438"
     CDS             complement(731..1153)
                     /locus_tag="ACFM8S_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFC6314502.1"
                     /translation="MALIKEPLDKAKQRNEELEAAEEAAAQEALGREQEAARVSEWEE
                     RYKLSRSEFEQFWKGLPQTVQNKLQASQKTWKSGMDKICANNAKAEGETPNGIKVSEL
                     ACKTVETEARLEELYNRKKALIDEMVREADKKELPKRL"
     gene            complement(1296..2942)
                     /gene="pgi"
                     /locus_tag="ACFM8S_001439"
     CDS             complement(1296..2942)
                     /gene="pgi"
                     /locus_tag="ACFM8S_001439"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFC6314503.1"
                     /translation="MKHLHDLPAWSKLWNHFDDSKTLHMREMFEQDPQRAERYWLQVG
                     GLTLDYSKNRINDETMSLLFELAREAGVPERMRQMFHGEKINTTENRAVLHVALRNRT
                     NSPIVVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTNQVITDVVNIGIGGSDLGPL
                     MMCTALKPFGHPRLNMHFVSNVDGSQLRDVLSKVHPETTLFIIASKTFTTQETLTNAL
                     TAREWFLNHAGDEEAVAKHFAAVSTNQKAVAEFGIDTANMFEFWDWVGGRYSLWSAIG
                     LPIMLYLGEENFIEMLNGAHLMDQHFINTPLERNLPVILALIGIWYINYYGGGSHVIA
                     PYDQHLHRLPKFIQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLHQ
                     GTHITPIDLIASLEKRSNLPGHHEILLANVFAQAEAFMRGKTPDEVRAELKAQGMDEA
                     RIEELVPHKTFSGNRPTNLILMDKVNPRNMGSLIAMYEHKTFVQGIIWGINSFDQWGV
                     ELGKQLAKTILGELTGETGSQKHDSSTERLINLYLQTNRK"
     gene            complement(3063..3911)
                     /gene="hexR"
                     /locus_tag="ACFM8S_001440"
     CDS             complement(3063..3911)
                     /gene="hexR"
                     /locus_tag="ACFM8S_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFC6314504.1"
                     /translation="MLSKISESLANLSGAERKVAECALAEPKWFVHAAVAEIAERASV
                     SQPTVIRFCRSLGYKGLPEFKLALSASIGHEGMPYVHEELNADDDMASVVEKVLGNAA
                     ASLLGERRFLKESELENAIATLMHARRVEFYGVGNSGIVAQDAQHKFFRFGMSTVAYV
                     DTHTQLMAASVLSDQDVLVAISNTGSSIELLDAVSIAKENGASVIALTRNDSPLAQLA
                     DCVLSVATQENAELYTPMVSRLLQLAVIDILAIGLALRLGDAASLQLQKSKKSIHNKH
                     IDYDKD"
     gene            complement(3961..4947)
                     /locus_tag="ACFM8S_001441"
     CDS             complement(3961..4947)
                     /locus_tag="ACFM8S_001441"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFC6314505.1"
                     /translation="MSSTPNKQAGYPRLVADIGGTNARFALETAPRVIEKAAVLPCKD
                     YDTVTDAVRAYLNQSGATAVRHAAFAIANPILGDWVQMTNHHWAFSIETTRQTLGLDT
                     LILLNDFTAQALAVTQTSSKDLMQVGGQKPVEFAPKAVIGPGTGLGVSGLVHSHAGWV
                     ALAGEGGHTSFPPFDDMEVLIWQYAKNKYGHVSAERFLSGAGLSLVYEALAAKQKAKP
                     AKLMPSEITEKALSGASPLCRQTLDIFCAMLGTVASNLALTLGARGGVYLCGGIIPRV
                     LEYFKTSPFRSRFENKGRFEAYLAAIPVYVVLSEFPGISGAAAALDNHLRNV"
     gene            complement(4928..5623)
                     /gene="pgl"
                     /locus_tag="ACFM8S_001442"
     CDS             complement(4928..5623)
                     /gene="pgl"
                     /locus_tag="ACFM8S_001442"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFC6314506.1"
                     /translation="MFVWHEYENAAEAAQSLADAVADALQGALDEKGGAVLAVSGGRS
                     PIAFFNALSQKDLDWKNVGITLADERIVPTVHADSNTGLVREYLLKNKAEAAMWIPMV
                     EDGKTETELHPDAVVDYALKHYKQPDVLVLGMGNDGHTASIFPKAPQFQTAIDGSAGV
                     ALVHTTPVTAPHERVSMTLDAIAHTGHVFLAIRGEEKKAVFDQAAQGENREYPINLVL
                     NHQGVNCHVFYAE"
     gene            complement(5799..7244)
                     /gene="zwf"
                     /locus_tag="ACFM8S_001443"
     CDS             complement(5799..7244)
                     /gene="zwf"
                     /locus_tag="ACFM8S_001443"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFC6314507.1"
                     /translation="MSTQTNFDLVLFGATGDLAMRKLLPCLYQAHVAGLLHPEGRILG
                     VSRSELDTEGFLAKVETSSKIHVKENFSDEAWASFVERFAYLKVDVTQPDDFAALGDL
                     VKARKETDNVVIYLSTAPKFFAQTCENLAKIGLNADNVRVVLEKPLGTDLASSQQINT
                     DVARYFKEGQIYRIDHYLGKESLQNLLALRFANVMFEPLWNNKYIESVQLTIAEQLGV
                     EERGEFYDITGALRDMVQNHLMQMLCMTAMEAPASLDADAVRDEKVKVIKSLKPLTVE
                     SVNENVVRGQYTAARGMNGYLEEINVPQDSFTETYVAIKAEIENERWKGVPFYLRTGK
                     RMAGKVAEIVLNFKDLNSHIFEGSRTAPNRLVIELQPYESVRLYTQMKTPGAGNKVET
                     VPLATDLGKALEGRRAEAYERLLLDVINGKLALFNRRDELEAAWEYVMPILENWTNNT
                     TPPHGYGAHSWGPEAARELLAHDGHKWHEEQ"
BASE COUNT         1638 a         1995 c         1998 g         1935 t
ORIGIN      
        1 aagttaccgg tctaaatgcc gtcaaatcgg ataggcaaaa accggctcaa tatgttgggc
       61 aacatccgga tgcctatctg catgaaatcg ccaaacattt tgattgtacg gcagccaccg
      121 tttgctatgc actcaaacag atggggataa cgcgcaaaaa aaagaccacc acttacaaag
      181 aacaagaccc ggccaaaata acgcattatt tgacacagct ggccgaattt tccgactacc
      241 aacgtgttta tttggatgaa acaggatttg accgctacct gttccgtccc tatacccgca
      301 gcctgaaagg gcaaatagtg aaagcgcaga taagtggaaa aagatatagt ggattaacaa
      361 aaatcaggac aaggcggcga agccgcagac agtacaaata gtacggaccg attcacttgg
      421 tgcttcagca ccttagagaa tcgttctctt tgagctaagg cgagccaacg atgtatcggt
      481 ttaaatttaa ttcactataa aaacgacaaa aacgcaaaag ccgccgacat tcccgcatcc
      541 aagtttcagt caatcagata accttggatt tctttggttt tcgcattgat ttctctggta
      601 cggcagtcag attgtgccac gccgtattcg tcgccgtcag cgcatttggc attcagaccg
      661 ttttgccagt tgcggtactc gggcatgacg gtttcggcaa tatcggcggg cagtgcctga
      721 accgcgctgt tcagagccgc tttggcagtt ctttcttgtc cgcttccctg accatttcgt
      781 cgataagggc ttttttacgg ttgtacagct cttccaagcg tgcttcggtt tccaccgttt
      841 tgcatgccag ttcgctgact tttatgccgt ttggcgtttc accttcggct ttcgcattgt
      901 tggcacagat tttgtccata ccgcttttcc atgttttctg cgaagcttgc agtttattct
      961 gtacagtttg cggcaatcct ttccagaact gctcgaactc gctgcgcgac aacttgtagc
     1021 gttcttccca ttcggatacg cgggcggctt cctgctcccg acccaatgcc tcctgcgccg
     1081 ccgcttcttc tgccgcttca agttcttcgt tcctttgttt cgctttgtcc aacggctctt
     1141 taatcagtgc catagctgcg gaacatatat gtttaaattt atgcaaacca tcatatcggg
     1201 attgcacacg ctctgcaagt ttaccgacgg ttttcctgtt cgataaaaat gccgtttgaa
     1261 acggtcggcg ttcagacggc atttttccgc aggttttatt tgcggttggt ctgcaggtag
     1321 aggttgatca ggcgttcggt cgagctgtcg tgcttttgcg acccggtttc gccggtcagt
     1381 tcgcccaaaa tggttttagc cagttgtttg ccgagttcca cgccccactg gtcgaagctg
     1441 ttgatgcccc aaatgatgcc ttgtacgaag gttttgtgtt cgtacatggc aatcaggctg
     1501 cccatattac gcgggttgac cttgtccatc aggatgaggt tggtcgggcg gttaccggag
     1561 aaggttttgt gcgggaccag ctcttcgatg cgcgcctcat ccataccctg cgctttgagt
     1621 tcggcgcgga cttcgtcggg ggttttgccg cgcataaagg cttctgcttg ggcgaagacg
     1681 ttggcaagca ggatttcgtg gtgtccgggc aggttgctgc gtttttcaag cgaggcaatc
     1741 aggtcgatgg gggtaatgtg cgtgccttgg tgcagcagtt ggaagaaggc gtgctggccg
     1801 ttaatgcccg tttcgcccca gataatcggc gaggtttcgt gtccgactgc tttgccgtcc
     1861 aacgtaacct gtttgccgtt actttccata tcgagctgct ggatgaattt gggcaggcgg
     1921 tgcaaatgtt ggtcgtaagg cgcgatgacg tggctgccgc cgccgtagta gttgatatac
     1981 cagatgccga tgagggcgag aatgacgggc aggttgcgct cgagcggtgt gttgatgaag
     2041 tgttggtcca tcaggtgcgc gccgttgagc atttcgatga agttttcttc gccgagatac
     2101 agcataatcg gcaatccgat ggcggaccac aggctgtacc gaccgccgac ccaatcccaa
     2161 aattcaaaca tattggcggt gtcgatgccg aattcggcga cggctttttg attggtggaa
     2221 acggcggcga agtgtttggc aacggcttct tcgtcgcccg catgattcaa aaaccattcg
     2281 cgcgcggtca gcgcgttggt cagcgtttcc tgcgtggtaa atgttttgga ggcgatgatg
     2341 aacagcgtgg tttcggggtg gactttggac aatacgtcgc gcagttgcga gccgtccacg
     2401 ttggagacga agtgcatatt gaggcgcgga tgaccgaaag gtttgagcgc ggtacacatc
     2461 atcagcggac ccaaatccga tccgccgatg ccgatgttga caacgtcggt aatgacttgg
     2521 ttggtatagc ccagccagct tccgctgcgg acttcgtgtg caaattcgcc catacgttgc
     2581 aaaacgcggt tgactttggg catcacatct tcaccgtcaa ccacaatcgg cgaattggtg
     2641 cggttgcgaa gggcgacatg caggacggcg cggttttcgg tggtattgat tttttcgccg
     2701 tggaacatct gccgcatccg ctccggcacg cctgcttctc gggcaagctc gaacaaaagc
     2761 gacatggttt cgtcgttgat gcggtttttg gagtagtcca gcgtcagtcc gccgacttgc
     2821 agccagtagc gttccgcacg ctgcgggtct tgctcgaaca tttcgcgcat atgcaatgtt
     2881 ttgctgtcgt caaagtggtt ccacaatttc gaccatgcgg gtaagtcgtg aaggtgtttc
     2941 atctatatgc tcctgaatga ggttttttgt tgtgggatga aaaggctgcc ggaaactgcc
     3001 gcaagccgcc gacgaccgtt gttcggcatt tcagacggca tttgtgggat gccgtctgaa
     3061 ggtcaatctt tgtcgtaatc gatgtgcttg ttgtgtatgc tttttttgct tttctgcaat
     3121 tgcaggctgg cagcatcgcc caagcgcagg gcaagtccga tggcaaggat gtcgatgacg
     3181 gcaagctgca agaggcggga aaccatgggc gtgtagagtt cggcattttc ctgtgtggca
     3241 acgctcaaca cgcagtcggc aagttgcgcc agaggcgaat cgttgcgggt cagtgcgatg
     3301 acggacgcgc cgttttcttt ggcgatgctg accgcatcca aaagttcgat agacgaaccc
     3361 gtgttggaaa tggcaaccaa aacatcctga tcgctcaaaa cggatgccgc catcagctgc
     3421 gtgtgcgtat cgacataggc gaccgtggac attccgaaac ggaaaaactt gtgctgcgcg
     3481 tcctgcgcga cgataccgga attgccgaca ccgtaaaact cgacgcggcg ggcgtgcatc
     3541 agcgtggcaa tggcgttttc cagctccgac tctttcagga agcggcgttc gcccaacagc
     3601 gaggcggcgg cattgcccaa cactttctcg accacgcttg ccatatcgtc gtcggcgttg
     3661 agttcttcgt ggacataggg cataccctca tgaccgatgc tggcggacaa ggcgagcttg
     3721 aactcgggca gccctttata acccaagctg cggcagaatc ggatgacggt cggctggctg
     3781 acggacgcac gttcggcaat ttcggcaacg gcggcatgga cgaaccattt gggttccgcc
     3841 aatgcacatt cggcgacttt gcgttccgca ccggaaaggt ttgccagtga ttcgctgatt
     3901 ttgcttaaca taatgatatg cccttcgata atgcagcccc gctgcaagga gccgctgtgg
     3961 ttaaacgttt ctcaaatggt tgtcgagagc cgcagccgca ccggaaattc cgggaaactc
     4021 gctcaagacg acatacacgg gaatcgcggc aagatatgct tcaaacctgc ccttgttctc
     4081 gaaacggctg cggaacgggg aagttttgaa atattccaac acgcggggaa taatgccgcc
     4141 gcacaggtac acgccgccgc gcgcgcccag cgtcagggcg aggttggaag caaccgtgcc
     4201 gagcatggcg cagaagatgt ccaaagtctg acggcacaaa ggcgacgcgc cgctcaaagc
     4261 cttttccgtg atttcagacg gcatcagttt ggcgggtttg gctttctgtt ttgcagccaa
     4321 agcctcgtaa accaagctca agcccgcgcc gctcaaaaag cgttcggcgg aaacatggcc
     4381 gtatttgttt ttggcgtact gccaaatcag cacttccata tcgtcaaacg gcgggaaact
     4441 ggtatgcccg ccctcgcccg ccaaagccac ccagcctgcg tggctgtgca ccaatccgct
     4501 cacgcccagg ccggtaccgg ggccgataac ggctttgggg gcaaattcga caggcttttg
     4561 cccgcctacc tgcatcaggt ctttgcttga agtctgcgtt accgccaatg cctgcgcggt
     4621 aaagtcgttc aaaaggatga gggtgtccag ccccaaagtc tgacgggtgg tttcgatgga
     4681 aaacgcccaa tggtggttgg tcatctgcac ccagtcgccc aaaatcgggt tggcgatggc
     4741 aaatgccgcg tgccgtacgg ctgttgcacc gctttgattc agataggcac gcaccgcatc
     4801 ggtaaccgta tcgtagtctt tacacggaag cacggcggct ttttcaatga cgcgcggcgc
     4861 ggtttccagc gcaaagcgtg cattcgtccc gccgatatcg gcgaccagtc ggggatatcc
     4921 ggcttgttta ttcggcgtag aagacatggc agttcactcc ttgatggttc aaaacgaggt
     4981 tgatcggata ttcgcggttt tcgccttgtg cggcttggtc gaacacggct tttttctctt
     5041 cgccccgtat cgccaaaaac acatgccccg tatgggcaat cgcatccaag gtcatactga
     5101 cgcgctcgtg cggcgcggta acgggcgtgg tatgcaccag cgcgacacct gccgaaccgt
     5161 cgattgccgt ctgaaactgc ggagctttcg ggaaaatcga agccgtatgc ccgtcgtttc
     5221 ccatacccaa aaccaaaaca tcgggctgtt tgtaatgttt cagtgcataa tcgacaacag
     5281 catcgggatg taattcggtt tcagtttttc cgtcttccac cataggaatc cacattgccg
     5341 cttccgcttt gttcttcaac aggtattcgc gcaccaaacc ggtattgctg tcggcgtgga
     5401 cggtcggcac gatgcgttca tctgccaagg tgatgccgac gtttttccaa tccaaatctt
     5461 tttgcgacag ggcgttgaaa aatgcaatcg gcgaacgtcc gccggaaact gccaacaccg
     5521 caccgccctt ctcgtccagt gcgccctgca aagcatccgc cactgcgtca gccaaagact
     5581 gcgccgcttc tgccgcattt tcgtattcgt gccaaacaaa catatttgtg tccttttttt
     5641 atttcagacg gcatattccg ttatggaaac gggttgagca atatgccggt cgaacagttg
     5701 tttatgcttt tgataccaaa tatcgggact gctttttact gctttacttg cgttcagacg
     5761 gcatttcaaa ccccatgccg tccgaacgca ttattgtatt actgctcttc gtgccacttg
     5821 tgtccgtcgt gcgccaatag ttcgcgcgcg gcttcaggcc cccacgagtg tgcgccgtag
     5881 ccgtgcggcg gcgtggtgtt atttgtccag ttttccaaaa tcggcatcac atattcccac
     5941 gcggcttcga gttcgtcgcg gcggttgaac aaagcgagtt tgccgttaat cacatccagc
     6001 agcaggcgct cgtaagcttc cgcgcggcgg ccttccaatg ctttgcccaa atcggttgcc
     6061 agcggcacgg tttcgacctt atttcctgcc cccggggttt tcatctgcgt atagaggcgc
     6121 acggattcat atggttgcaa ctcgataacg agccggttgg gcgcggtgcg gctgccttca
     6181 aaaatatggc tgttcaaatc tttgaagttc aaaacgattt ccgccacttt gcccgccatg
     6241 cgtttgccgg tacgcaggta gaagggaacg cccttccagc gttcgttttc gatttcggct
     6301 ttaatggcga cgtaggtttc ggtaaagctg tcttgcggaa cgttgatttc ttcaagatag
     6361 ccgttcatgc ctctggcggc ggtatattgt ccgcgcacga cgttttcatt gacagactcg
     6421 acggtcagcg gcttcaatga cttgatgact ttgacttttt catcgcgcac cgcgtcggca
     6481 tccaagctgg cgggggcttc catcgcagtc atgcacagca tttgcatcaa gtggttttgc
     6541 accatatcgc gcaaggcgcc agtaatgtcg taaaactcgc cgcgctcttc cacaccgagc
     6601 tgttcggcga tggtcaactg cacgctttcg atgtatttat tgttccacag cggctcgaac
     6661 attacattgg caaaacgcag cgcaagcagg ttttgcaggc tttctttgcc aaggtagtgg
     6721 tcgatgcggt aaatttgccc ttctttgaaa taacgcgcaa catcggtatt gatttgctgg
     6781 gaagaagcca aatccgtacc caacggtttt tccaaaacca cacgcacgtt gtcggcgttc
     6841 aaaccgattt tggcaaggtt ttcacatgtt tgcgcgaaga atttcggcgc ggtggaaaga
     6901 tagatgacga cattgtcggt ttctttgcgc gctttgacca aatcgcccaa agcggcaaaa
     6961 tcgtccggct gcgtaacatc gactttgaga tatgcgaaac gttcgacaaa cgatgcccaa
     7021 gcctcatcgg aaaaattttc tttcacatgg attttggaac tggtttccac cttcgccaga
     7081 aaaccttcgg tatccaactc gctgcggctg acccccaaaa tacgcccttc gggatgaagc
     7141 agaccggcaa catgcgcctg gtacagacag ggcaacagct tgcgcatcgc caaatcgccg
     7201 gtcgcaccga acaacaccaa atcaaaattt gtttgtgtac tcatcgtatt atctcgtcag
     7261 gaaagaattt ttcgatgccg tttgaaacct gttttcccca tcacgctgca tcgcaatatc
     7321 ggaaacaaag gcaggcggca taatgagtag taatactaca caccgctaca ctttttgtct
     7381 attcccattt ttacaattta tttgacctag tccaaaaatc gggcaggttt cccctattcc
     7441 gttacaacaa tcgaaagatt ctgcgattta aatcaaattt cttttcgatg tctgattttt
     7501 ttgtaacaaa attacaaatt ttgtactata ataacacccg cttcccactt tcagacggta
     7561 tacctt
//
LOCUS       DAXHSF010000100         7555 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.100, whole genome shotgun sequence.
ACCESSION   DAXHSF010000100 DAXHSF010000000
VERSION     DAXHSF010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7555
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.100"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..419
                     /locus_tag="ACFM8S_001444"
     CDS             <1..419
                     /locus_tag="ACFM8S_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFC6314508.1"
                     /translation="EAEWVGSLFGLHKSFGFLTLTVITLRIVWAVANRAKRPQSDSKA
                     AAAGHGILYLLMLAVPVIGMIRQYGSGRGPLKVFGVEVMQGSPEKIEWMANLGNTFHG
                     NLGWLLFAAVVGHVAMVVVHRVQGKDVLYRMTGRVR"
     gene            complement(454..1329)
                     /gene="rsmI"
                     /locus_tag="ACFM8S_001445"
     CDS             complement(454..1329)
                     /gene="rsmI"
                     /locus_tag="ACFM8S_001445"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFC6314509.1"
                     /translation="MFQKHLQKASDSVVGGTLYVVATPIGNLADITLRALAVLQKADI
                     ICAEDTRVTAQLLSAYGIQGKLVSVREHNERQMADKIVGYLSDGMVVAQVSDAGTPAV
                     CDPGAKLAGRVREAGFKVVPVVGASAVMAALSVAGVEGSDFYFNGFVPPKSGERRKLF
                     AKWVRAAFPIVMFETPHRIGATLADMVELLPERRLMLAREITKTFETFLSGTVGEIQT
                     ALVADGNQSRGEMVLVLYPAQDEKHEGLSESAQNIMKILTAELPTKQAAELAAKITGE
                     GKKALYNLALSWKNK"
     gene            1383..1730
                     /locus_tag="ACFM8S_001446"
     CDS             1383..1730
                     /locus_tag="ACFM8S_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFC6314510.1"
                     /translation="MRLNHKQGEAGEDAALAFLQSQGCTLLARNWHCAYGEIDLIVKN
                     GGMILFVEVKYRKNRQFGGVAYSISPSKLLKLQRSVEYYLQQNRLTNVPCRLDAVLIE
                     GNRPPEWIQNITG"
     gene            1735..2328
                     /locus_tag="ACFM8S_001447"
     CDS             1735..2328
                     /locus_tag="ACFM8S_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HFC6314511.1"
                     /translation="MTTLQERVAAHFAESIRAKQEAEKVLVEPTVQAAELMLQCLMND
                     GKILACGNGGSAADAQHFAAEMTGRFEKERMELAAVALTTDTSALTAIGNDYGFNHVF
                     SKQVRALGRAGDVLVGISTSGNSANVIEAIKAAHERDMHVIALTGRDGGKIAAMLKDT
                     DVLLNVPYPRTARIQENHILLIHAICDCIDSVLLEGM"
     gene            2392..3000
                     /locus_tag="ACFM8S_001448"
     CDS             2392..3000
                     /locus_tag="ACFM8S_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HFC6314512.1"
                     /translation="MKPKPHTVRTLIAAIFSLALSGCVSAVIGSAAVGAKSAVDRRTT
                     GAQTDDNVMALRIETTARSYLHQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFV
                     GQIARSEQAAEGVYNYITVASQPRSLGDITNDTWNTSKVRATLLGISPTTQARVKIVT
                     YGNVTYVMGILTPEEQAQITQKVSTTVGVQKVITLYQNYVQR"
     gene            3008..3655
                     /locus_tag="ACFM8S_001449"
     CDS             3008..3655
                     /locus_tag="ACFM8S_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314513.1"
                     /translation="MPSEPPSDGIARHPKSTIKMAKKPNKPFRLTPKLLIRVVLLICI
                     TAIGALAIGIVSTFNPNGGKTLQTEPQHTDSPRETEFWLPNGVVGQDAAQPEHHAASS
                     EPAQPDGTDESGSGLPSPAAPKKNRVKPQPADTAQTDRQPDDAGTQAENTLKETPVLP
                     TNVPRPEPRKETPEKQAQPKETPKEKETPKENHTKPDTPKNTPPKPHKEILDNLF"
     gene            3695..4474
                     /gene="map"
                     /locus_tag="ACFM8S_001450"
     CDS             3695..4474
                     /gene="map"
                     /locus_tag="ACFM8S_001450"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFC6314514.1"
                     /translation="MNGIIIKTPEEIEKMRELGKLVAEALDYIGQFVKPGVTTDEIDK
                     LVYDYHVNVQGGYPAPLHYGNPPYPKSCCTSVNHVICHGIPDDKPLKEGDIINIDLTI
                     KKDGFHGDSSRMFTVGKVSPIAQRLIDVTHASMMAGIEAVKPGATLGDVGYACQQVAE
                     NAGYSVVQEFCGHGIGRGFHEAPQVLHYGKKGQGPVLKPGMIFTVEPMINQGKRHLRI
                     LNDGWTVVTKDRSLSAQWEHEVLVTETGYEILTVSPASGKP"
     gene            4629..4928
                     /locus_tag="ACFM8S_001451"
     CDS             4629..4928
                     /locus_tag="ACFM8S_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC6314515.1"
                     /translation="MKIFENIEDVKAIRKKTGLNQIDFWGKVGVTQSGGSRYETGRKM
                     PKPVRELLRLVHIECIDLAKVNKKDMEIAALLKKHHPDLYAELSKQTKSERKKQS"
     gene            5166..5540
                     /locus_tag="ACFM8S_001452"
     CDS             5166..5540
                     /locus_tag="ACFM8S_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFC6314516.1"
                     /translation="MKLLTTAILSSAIALSSMAAAAGTNNPTVAKKTVSYVCQQGKKV
                     KVTYGFNKQGLTTYASAVINGKRVQMPVNLDKSDNVETFYGKEGGYVLGTGVMDGKSY
                     RKQPIMITAPDNQIVFKDCSPR"
     gene            complement(5728..7194)
                     /locus_tag="ACFM8S_001453"
     CDS             complement(5728..7194)
                     /locus_tag="ACFM8S_001453"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HFC6314517.1"
                     /translation="MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALE
                     SSNAWNNAGTGHSALCELNYAPLGANGIIDPARALNIAEQFHVSRQFWATLVAEGKLE
                     DNSFINAVPHMSLVMNEDHCSYLQKRYDAFKTQKLFENMEFSTDRNKISDWAPLMMRG
                     RDENQPVAANYSAEGTDVDFGRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLK
                     TADTRNPDGQLTLRTRFLFLGAGGGALTLLQKSGIPEGKGYGGFPVSGLFFRNSNPET
                     AEQHNAKVYGQASVGAPPMSVPHLDTRNVDGKRHLMFGPYAGFRSNFLKQGSLMDLPL
                     SIHMDNLYPMLCAGWANMPLTKYLLGELRKTKEERFASLLEYYPEANPDDWELITAGQ
                     RVQIIKKDSEKGGVLQFGTEIVAHADGSLAALLGASPGASTAVPLMIRLMHQCFPERA
                     PSWEDRLKELVPGYGIKLNENPERADEIIAYTAKVLDI"
BASE COUNT         1951 a         2278 c         1843 g         1483 t
ORIGIN      
        1 aagaggcgga atgggtgggc agcctgttcg gcctgcacaa atctttcggt ttccttacgc
       61 tgacggtgat tacattgcgc atcgtgtggg cggttgccaa ccgcgccaag cgtccgcaaa
      121 gcgacagcaa ggctgcggcg gcaggacacg gcattctgta tctgctcatg cttgccgttc
      181 ccgttatcgg catgatccgc caatacggca gcggccgcgg cccgttgaaa gtgttcggcg
      241 ttgaggtgat gcagggttcg ccggaaaaaa tcgagtggat ggcaaacttg ggcaacacgt
      301 tccacggcaa tttgggctgg ctgctgtttg ccgccgtcgt cggacacgtc gccatggtcg
      361 tcgtccaccg tgttcaaggc aaagacgtgc tgtaccgcat gacggggcgt gtccgttgat
      421 tccgttcgca ttatggtgcc ggtccgtccg gcatcatttg tttttccaag acagtgccag
      481 attgtacaaa gctttttttc cctcgcccgt gattttggcg gcaagctccg ccgcctgttt
      541 ggtcggcagc tcggctgtga ggattttcat gatgttttgc gcggactcgg acaagccttc
      601 gtgtttttca tcctgcgccg gataaagcac caacaccatc tcgccgcgcg attggttgcc
      661 gtccgccacc aatgccgtct gaatttcccc aaccgtgccg cttaagaacg tttcaaacgt
      721 tttcgtaatt tcgcgcgcca gcatcagacg gcgttcgggg agcagttcca ccatatcggc
      781 aagcgtcgcc ccgattcggt gcggcgtttc aaacatgacg ataggaaatg ccgcccgcac
      841 ccatttggca aacagtttcc tacgttcgcc cgatttcggc ggtacaaaac cgttgaaata
      901 aaaatcggat ccttccacac cggccacgct caaagccgcc atcaccgcgc ttgcgcccac
      961 gacgggaacg actttaaacc cggcctcacg cacgcggccg gcgagtttgg cacccgggtc
     1021 gcacacggcc ggcgtacccg catcggaaac ctgtgccaca accatgccgt ctgaaagata
     1081 gccgacaatc ttgtccgcca tctgccgttc gttgtgttcg cgcacactga caagtttgcc
     1141 ctgaatgccg tacgcgctca aaagctgcgc ggtaacgcgc gtgtcttcgg cacagatgat
     1201 gtccgccttt tgcaataccg ccaaagcgcg cagggtaatg tccgccaaat tgccgatggg
     1261 cgtggcaacc acgtataatg tccctccgac gacgctgtcg gaggctttct gcaagtgttt
     1321 ctgaaacata aaaaatgccg tctgaaaaac aaacattata aattataaag gttaaaccga
     1381 ttatgcgcct aaaccacaaa cagggcgagg caggggaaga tgccgcgctt gccttcctcc
     1441 aatcccaagg ctgcacactg cttgcccgca actggcactg cgcctacggc gaaatcgacc
     1501 tgattgtcaa aaacggcggt atgattctgt ttgttgaagt aaaataccgc aaaaatcggc
     1561 aattcggcgg tgtcgcatac agcatttccc catccaaatt attgaaactg caacgaagtg
     1621 tagagtatta tctgcaacag aacaggttga caaacgtacc gtgccgcctc gatgcggtac
     1681 tgattgaagg aaaccgcccg cccgagtgga tacagaatat tacaggttga cgatatgacg
     1741 acattacaag aacgcgttgc cgcccatttt gccgaaagca tccgtgccaa acaggaagcc
     1801 gaaaaagtac tggtcgagcc gaccgtacag gctgccgagc tgatgctgca atgcctgatg
     1861 aacgacggca agattctggc gtgcggcaac ggcggttcgg ccgccgatgc ccagcacttc
     1921 gccgccgaaa tgaccggacg ctttgaaaaa gagcgcatgg aactcgccgc cgtcgccctg
     1981 accaccgaca catccgcgct gaccgccatc ggtaacgact acggcttcaa ccacgtcttc
     2041 agcaaacagg tacgcgccct cggacgtgca ggcgacgtct tggtcggcat ttccacttcc
     2101 ggcaactccg ccaacgtcat cgaagccatt aaagcggcac acgaacgcga tatgcacgtc
     2161 atcgccctga ccggccgcga cggcggcaaa atcgccgcca tgctcaaaga caccgacgtt
     2221 ctgctcaacg tcccctatcc gcgcaccgcc cgcattcagg aaaaccacat cctgctgata
     2281 cacgccatat gcgactgtat cgactccgta ctgctggaag gaatgtaacc cttttcagac
     2341 ggcatggcgc aaagcaatgc cgtctgaaac gcccaagaaa ggaagcaccc gatgaaaccc
     2401 aaaccgcaca ccgtccgcac cctgattgcc gccattttca gccttgccct tagcggctgc
     2461 gtcagcgcag taatcggaag cgccgccgtc ggcgcgaaat ccgccgtcga ccgccgaacc
     2521 accggcgcgc aaaccgacga caacgttatg gcgttgcgta tcgaaaccac cgcccgttcc
     2581 tacctgcacc aaaacaacca aaccaaaggc tacacgcccc aaatctccgt cgtcggctac
     2641 aaccgccacc tgctgctgct cggacaagtc gccaccgaag gcgaaaaaca gttcgtcggt
     2701 cagattgcac gttccgaaca ggccgccgaa ggcgtgtaca actacattac cgtcgcctcg
     2761 caaccgcgct ccctcggcga catcaccaac gacacctgga atacctccaa agtccgcgcc
     2821 acgctgctgg gcatcagccc caccacgcag gcgcgcgtca aaatcgttac ctacggcaac
     2881 gtaacctacg ttatgggcat cctcaccccc gaagaacagg cgcagattac ccaaaaagtc
     2941 agcaccaccg tcggcgtaca aaaagtcatc accctctacc aaaactacgt ccaacgctga
     3001 ctcggcaatg ccgtctgaac cgccttcaga cggcattgcc cgacacccca aaagcacaat
     3061 caaaatggca aaaaaaccga acaaaccctt caggctgacc cccaaactcc tgatacgcgt
     3121 cgtattgctc atctgtatca ccgccatcgg cgcattggca ataggcatcg tcagcacatt
     3181 caacccgaac ggcggcaaaa ccctccaaac cgaaccgcaa cacaccgaca gcccccgcga
     3241 aaccgaattc tggctgccaa acggcgtagt cggacaagat gccgcccaac ccgaacacca
     3301 cgccgcctca tccgaacccg cacagccgga cggcacagac gaaagcggca gcggactgcc
     3361 gtcccctgcc gcacccaaga aaaaccgggt caaaccgcaa cctgccgaca cagctcaaac
     3421 cgacaggcag ccggacgacg ccggaacaca agctgaaaac acactcaaag aaacccccgt
     3481 actgcccaca aacgtccccc gtcccgaacc ccgaaaagaa acacccgaaa aacaggcgca
     3541 gcccaaagaa acacccaaag aaaaagaaac gcccaaagaa aaccatacca aaccggacac
     3601 cccgaaaaac acgccgccca aaccccataa agaaattctc gacaacctct tctgacccgg
     3661 cacggcaggc acacccgcaa tccaaggaag cattatgaac ggcatcatca tcaaaacccc
     3721 cgaagaaatc gaaaaaatgc gcgagctggg caaactcgtc gccgaagccc tcgactacat
     3781 cggacaattc gtcaaacccg gcgtaaccac cgacgaaatc gacaaactcg tttacgacta
     3841 ccacgtcaac gtccaaggcg gctatcccgc ccccctgcac tacggcaacc cgccctaccc
     3901 caaatcctgc tgcacctccg tcaaccacgt catctgccac ggcattcccg acgacaagcc
     3961 gctcaaagaa ggcgacatta tcaacatcga cctcaccatc aaaaaagacg gcttccacgg
     4021 cgactccagc cgtatgttta ccgtcggcaa agtctccccc atcgcccaac gcctgatcga
     4081 cgtaacccac gcctccatga tggcgggcat agaagccgtc aaacccggcg cgacactggg
     4141 cgacgtaggt tacgcctgcc aacaggttgc cgaaaacgcc ggctattccg tcgtacagga
     4201 attctgcgga cacggcatcg ggcgcggttt ccacgaagcc ccgcaagtgt tgcactacgg
     4261 aaaaaaagga cagggccccg ttctaaaacc gggtatgatt tttaccgtcg aaccgatgat
     4321 caaccaaggc aaacgccacc tgcgtatcct caacgacggc tggacggtgg ttaccaaaga
     4381 ccgctccctc tccgcccaat gggaacacga agtcttggtg accgaaaccg gctacgaaat
     4441 cctcaccgtc agccccgcct ccggcaaacc ctgaagccgg acgtatccgc cccataaaaa
     4501 caaacaatgc cgtctgaaag aaacggcaga tatgatatat aatataaaaa caggcttgac
     4561 ccggcacatt acgaaaacaa agcaaatcgg aatttgcccc gcaaccagac aaacttaaag
     4621 gaagttttat gaaaatattt gaaaatatag aagatgttaa agccatccgt aaaaagaccg
     4681 ggctgaacca gatagacttc tggggcaagg tcggcgttac ccagtctgga ggctcgcgct
     4741 acgaaaccgg ccgcaaaatg cccaaacccg tacgcgaact gctccgcctc gtccatatcg
     4801 aatgcatcga tttggcgaaa gtcaacaaaa aagatatgga aatcgccgcc ctgttgaaaa
     4861 aacaccatcc cgacctgtat gccgaattgt ccaaacagac caagtccgaa agaaaaaaac
     4921 aaagttaaac cgcaacctcc ggatgcccga cagtttttca tttccgaaaa acgcaaacaa
     4981 tgccgtctga aacaccggac aggccgccgt atcccgcctg ccgcccctgc ctcaaaccgc
     5041 cgaaccgccc gaacccgcct ttttacaaac tttatccaat ttcctgttta tttcaggata
     5101 cgccgacatt agaatgtcaa acagctcgaa acgggcaaac tccacatcca tccaaaggaa
     5161 taaaaatgaa acttctgacc accgcaatcc tgtcttccgc aatcgcgctc agcagtatgg
     5221 ctgctgctgc cggcacgaac aaccccaccg ttgccaaaaa aaccgtcagc tacgtctgcc
     5281 agcaaggtaa aaaagtcaaa gtaacctacg gcttcaacaa acagggtctg accacatacg
     5341 cttccgccgt catcaacggc aaacgtgtgc aaatgcctgt caatttggat aaatccgaca
     5401 atgtggaaac attctacggc aaagaaggcg gttatgtttt gggtaccggc gtgatggatg
     5461 gcaaatctta ccgcaaacag cccattatga ttaccgcacc tgacaaccaa atcgtcttca
     5521 aagactgttc cccacgttaa tcaggcaaca aaaaacagcg ttttcagaaa tgaaaacgct
     5581 gtttttttga ccgttccatt attcgcaaaa gggaaaaaac gattacctgc cccgtgtatc
     5641 aaaacctgcc ctgccggatg aagggcataa ccggcaggga cggcgtcaac accatatggg
     5701 ggtacggctt ttcttgaaag attcggctta aatatccaat actttcgcgg tataggcgat
     5761 aatttcatcc gccctttcag ggttttcgtt caacttgatg ccgtaacccg gtaccagctc
     5821 tttcagacgg tcttcccaag acggggcgcg ctcggggaag cattggtgca tcagccggat
     5881 catcagcggc acagcggtcg atgcgcccgg cgacgcgccc agcaatgcgg cgagtgagcc
     5941 gtcggcgtgg gcgacaatct ccgtaccaaa ctggagcacg ccgccttttt cggagtcttt
     6001 tttaatgatt tggacgcgtt gccctgcggt gatgagttcc cagtcgtcgg ggtttgcctc
     6061 ggggtagtat tccagcaggg aggcgaagcg ttcttctttg gttttacgca attcgcccag
     6121 caggtatttg gtcagcggca tattcgccca gccggcgcac agcataggat agaggttgtc
     6181 catatggatg gacagcggca aatccataag cgagccttgc ttgaggaagt tggaacggaa
     6241 gcctgcgtaa gggccgaaca taaggtggcg tttgccgtcc acgttgcgtg tgtcgaggtg
     6301 cgggacggac atcggcggcg cgccgacgga agcctgcccg tacactttgg cgttgtgttg
     6361 ttcggcggtt tcggggttgc tgttgcggaa gaacaggccg gacacgggga agccgccgta
     6421 gcctttgcct tcggggatgc cggatttttg cagcagggtc agcgcgccgc cgcccgcgcc
     6481 gaggaagagg aagcgggtac ggagggtgag ctgcccgtcg gggttgcggg tatcggcggt
     6541 tttgagcacc cacgcgccgt cggattcgcg tttgatgtct tcgacgtggc ggttgaactc
     6601 ggtttttacg cccttgccct gcaaatattt caccatttgg cgcgtcagcc gtccgaaatc
     6661 gacatccgta ccttcggcgg agtagttggc ggcgacgggt tggttttcgt cccggccgcg
     6721 catcatcagc ggagcccaat cggaaatttt gttccgatcg gtggaaaatt ccatattttc
     6781 aaaaagtttt tgggttttaa acgcgtcata acgtttttga agataagaac aatggtcttc
     6841 attcatcacc aaagacatat gcggcacggc attgatgaag gaattgtctt ccaacttgcc
     6901 ttccgcgacc agcgtcgccc aaaactggcg gctgacatga aactgttcgg caatattgag
     6961 ggcgcgcgcc ggatcgataa tcccatttgc acccaacggc gcatagttca attcgcacag
     7021 cgcggaatgc cccgtgccgg cgttgttcca cgcgtttgac gattccaacg ccacatcttc
     7081 caagcgttca atcagggtga tttcccaaga cggttcgagt tctttgagca aaacgcccaa
     7141 agtcgcgctc ataatgccgc cgcccaccaa gacaacgtct gtcgcttcag ccatggttta
     7201 ctcctaaaaa acaggcatct tctgccctta tggttatttg ccgtactaca aacgcctgaa
     7261 tcgcaaaagc agggaaaacc ggcaatggtg tgtgtccgag tatgctgttt cggggttgga
     7321 atgcgttgca agcatggctt ccgacaccgc ttcaggggct tgtaatatgt tatcgtgaat
     7381 gtagtggatt ttactgggaa atgcaaagtt tttctgtcgc ccgccaagtc gggaaactgc
     7441 gaaatgaaaa ataaaaatag ttatttatct atatatatca aatttttaat agataaaaaa
     7501 tcaaaattgt ttatatatta atttttaaaa gattgtcagc atattgcgtt aagtt
//
LOCUS       DAXHSF010000101         7479 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.101, whole genome shotgun sequence.
ACCESSION   DAXHSF010000101 DAXHSF010000000
VERSION     DAXHSF010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7479
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.101"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(<1..4547)
                     /gene="hrpA"
                     /locus_tag="ACFM8S_001454"
     CDS             complement(<1..4547)
                     /gene="hrpA"
                     /locus_tag="ACFM8S_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion exoprotein HrpA"
                     /protein_id="HFC6314518.1"
                     /translation="MNKGLHRIIFSKKHSTMVAVAETANSQGKGKQAGSSVSVSLKTS
                     GDLCGKLKTTLKTLVCSLVSLSMVLPAHAQITTDKSAPKNQQVVILKTNTGAPLVNIQ
                     TPNGRGLSHNRYTQFDVDNKGAVLNNDRNNNPFLVKGGAQLILNEVRGTASKLNGIVT
                     VGGQKADVIIANPNGITVNGGGFKNVGRGILTTGAPQIGKDGALTGFDVRQGTLTVGA
                     AGWNDKGGADYTEILARAVALQGKLQGKNLAVSTGPQKVDYASGEISAGTAAGTKPTI
                     ALDTAALGGMYADSITLIANEKGVGVKNAGTLEAAKQLIVTSSGRIENSGRIATTADS
                     TEASPTYLSIETTETGAAGAFISNGGRIESKGLLVIDTGEDIGLRNGAVVQNNGNRPA
                     ATVLNAGRNLVIESKANVNNAKGSTNLSTGGRTVIKEASIQTGTTVYSSSKGNAELGN
                     NTRITGADVTVLSNGTISSSAVIDAKDTAHIEAGKPLSLEASTVTSDIRLNGGSIKGG
                     KQLALLADDNITAKTTNLNTPGNLYVHTGKDLNLNVDKDLSAASIHLKSDNAAHITGT
                     SKTLTASKDMGVEAGSLNVTNTNLRTNSGNLHIQAAKGNIQLRNTKLNAAKALETTAL
                     QGNIVSDGLHAVSADGHVSLLANGNADFTGHNTLTAKADVNAGSVGKGRLKADNTNIT
                     SSSGDITLVAGNGIQLGDGKQRNSINGKHISIKNNGGNADLKNLNVHAKSGALNIHSD
                     RALSIENTKLESTHNTHLNAQHERVTLNQVDAYAHRHLSITGSQIWQNDKLPSANKLV
                     ANGVLALNARYSQIADNTTLRAGAINLTAGTALVKRGNINWSTVSTKTLEDNAELKPL
                     AGRLNIEAGSGTLTIEPANRISAHTDLSIKTGGKLLLSAKGGNAGAPSAQVSSLEAKG
                     NIRLVTGETDLRGSKITAGKNLVVATTKGKLNIEAVNNSFSNYFPTQKAAELNQKSKE
                     LEQQIAQLKKSSPKSKLIPTLQEERDRLAFYIQAINKEVKGKKPKGKEYLQAKLSAQN
                     IDLISAQGIEISGSDITASKKLNLHAAGVLPKAADSEAAAILIDGITDQYEIGKPTYK
                     SHYDKAALNKPSRLTGRTGVSIHAAAALDDARIIIGASEIKAPSGSIDIKAHSDIVLE
                     AGQNDAYTFLKTKGKSGKIIRKTKFTSTRDHLIMPAPVELTANGITLQAGGNIEANTT
                     RFNAPAGKVTLVAGEELQLLAEEGIHKHELDVQKSRRFIGIKVGKSNYSKNELNETKL
                     PVRVVAQTAATRSGWDTVLEGTEFKTTLAGADIQAGVGEKARADAKIILKGIVNRIQS
                     EEKLETNSTVWQKQAGRGSTIETLKLPSFESPTPPKLTAPGGYIVDIPKGNLKTEIEK
                     LAKQPEYAYLKQLQVAKNVNWNQVQLAYDKWDYKQEGLTRAGAAIVTIIVTALTYGYG
                     ATAAGGVAASGSSTAAAAGTAATTTAAATTVSTATAMQTAALASLYSQAAVSIINNKG
                     DVGKALKDLGTSDTVKQIVTSA"
     gene            complement(4657..6399)
                     /gene="hrpB"
                     /locus_tag="ACFM8S_001455"
     CDS             complement(4657..6399)
                     /gene="hrpB"
                     /locus_tag="ACFM8S_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion system transporter HrpB"
                     /protein_id="HFC6314519.1"
                     /translation="MKFFPAPCLLVILAVIPLKTLAADENDAELIRSMQRQQHIDAEL
                     LTDANVRFEQPLEKNNYVLSEDETPCTRVNYISLDDKTVRKFSFLPSVLMKETAFKTG
                     MCLGSNNLSRLQKAAQQILIVRGYLTSQAIIQPQNMDSGILKLRVSAGEIGDIRYEEK
                     RDGKSAEGSISTFNNKFPLYRNKILNLRDVEQGLENLRRLSSVKTDIQIIPSEEEGKS
                     DLQIKWQQNKPIRFSIGIDDAGGKTTGKYQGNVALSFDNPLGLSDLFYVSYGRGLVHK
                     TDLTDATGTETESGSRSYSVHYSVPVKKWLFSFNHNGHRYHEATEGYSVNYDYNGKQY
                     QSSLAAERMLWRNRLHKTSVGMKLWTRQTYKYIDDAEIEVQRRRSAGWEAELRHRAYL
                     NRWQLDGKLSYKRGTGMRQSMPAPEENGGDILPGTSRMKIITAGLDAAAPFILGKQQF
                     FYATAIQAQWNKTPLVAQDKLSIGSRYTVRGFDGEQSLFGERGFYWQNTLTWYFHPNH
                     QFYLGADYGRVSGESAQYVSGKQLMGAVVGFRGGHKVGGMFAYDLFAGKPLHKPKGFQ
                     TTNTVYGFNLNYSF"
     gene            complement(6547..6699)
                     /locus_tag="ACFM8S_001456"
                     /pseudo
     CDS             complement(6547..6699)
                     /locus_tag="ACFM8S_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117473.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
     gene            complement(6983..7285)
                     /locus_tag="ACFM8S_001457"
     CDS             complement(6983..7285)
                     /locus_tag="ACFM8S_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314520.1"
                     /translation="MTWVGGSGNAQQSSYYCEICESAGLNDTGNPDKSLGLPNNTFSN
                     PPDWGATIGALAGSRIGMPEFGTFASHAIENFDWSWYRRYREIAETIEREYSGGLP"
BASE COUNT         1730 a         1719 c         1819 g         2211 t
ORIGIN      
        1 gcagaagtga caatctgctt gaccgtatca ctggtgccga gatctttcaa cgctttgccg
       61 acatcacctt tattattgat gatggataca gctgcttggc tatacaagga ggctaaagca
      121 gcggtttgca tggcagtcgc tgtagaaacg gtagtagctg ctgctgtcgt tgtagcggct
      181 gttccggcag ctgcggctgt actacttcct gaagcggcta caccgcccgc tgcggttgcg
      241 ccgtatccat aagtcagtgc ggttacgatt atggtaacaa tcgctgcacc ggctctggtt
      301 aagccttcct gcttatagtc ccatttatcg taagccagtt gcacctggtt ccagttgacg
      361 tttttcgcta cttggagctg tttcagatag gcatactcgg gctgtttggc cagcttttcg
      421 atttcggttt tcaaattgcc tttcggaatg tcgacgatat agccaccggg ggcggtcagt
      481 ttgggcggag tagggctttc gaagctgggc agtttcagcg tttcgatagt gctgccgcgt
      541 ccggcctgtt tctgccatac ggttgagttg gtttctaatt tttcttccga ctggatacgg
      601 ttcacaatgc ctttgaggat aattttcgca tcggcacggg ctttttcgcc tacacctgcc
      661 tgaatgtccg caccggccag cgtggttttg aattcggtac cttcgagcac ggtatcccag
      721 cctgaacggg tggctgcagt ttgggcgacg acgcggacag gcaatttggt ttcgttcagt
      781 tcgtttttac tgtaattgct cttgcctacc ttgatgccga taaagcggcg gcttttttgg
      841 acatccaact cgtgcttgtg gatgccttct tctgccagca gttgcagctc ttcacccgca
      901 accagggtaa ctttacctgc aggggcattg aagcgggtgg tattagcttc gatgttgccg
      961 cctgcctgaa gcgttatgcc gttggcggtc agctcgacgg gggctggcat aatcaggtgg
     1021 tcgcgggtgc tggtaaactt ggtttttctg atgattttgc cgcttttacc tttggttttt
     1081 aagaaggtat aggcatcgtt ttgtccagcc tccagtacaa tatcactatg ggctttgatg
     1141 tctatgctgc ctgagggagc tttgatttcg gatgcaccga taataatacg tgcatcatcg
     1201 agtgccgcag ctgcatgaat acttaccccc gtacgtccgg tcaaacgtga aggcttgttc
     1261 agagcagctt tgtcgtagtg actcttgtag gtgggcttgc caatttcata ttggtcggtt
     1321 atgccgtcaa tcagaatagc agccgcctct gaatctgctg cctttggcaa tacgcctgcg
     1381 gcgtgaaggt tcagtttttt ggaagcggta atatcggaac cgctgatttc gatgccttgt
     1441 gcggaaatca agtcaatatt ttgtgcagaa agcttggctt gcaggtattc tttgcctttg
     1501 ggttttttac ctttaacttc cttgttgatg gcttgaatat agaaagcgag acggtcgcgt
     1561 tcttcttgca gggttggaat cagcttgctt ttaggcgagc tttttttcaa ctgcgcaatc
     1621 tgctgttcca attctttgga tttttggttg agttcagccg ctttttgtgt aggaaaataa
     1681 ttgctgaatg agttgtttac ggcttcgata ttcaacttgc ctttggtggt ggcgacaacc
     1741 aagtttttac cggctgtaat tttagaacct cttaaatctg tttctcctgt aaccagacgg
     1801 atattgcctt ttgcttccaa tgaggaaact tgagcactag gcgcacctgc atttcctcct
     1861 tttgcagaca acagcaattt tccgcctgtt ttgatgctca ggtcggtatg cgcactgatg
     1921 cggttggcag gttcgatggt taatgtgccg ctacctgctt caatattcag ccgtccggcc
     1981 aatggtttta attcggcatt atcttccaaa gttttggtcg aaacggtact ccaattgatg
     2041 ttgccgcgct tgactagggc ggtaccggca gtaaggttga ttgcacccgc tctcagcgtg
     2101 gtgttgtcgg caatttggga atagcgcgca ttgagtgcca atacaccgtt agccaccagc
     2161 ttgttggcag aaggcagttt gtcgttttgc caaatctggc tgccggtaat gcttagatga
     2221 cggtgtgcgt aggcatctac ttggttgagc gttacccgct cgtgttgtgc attaagatgc
     2281 gtattatggg tagactccag cttggtattt tctatgctca atgcccggtc ggaatgaatg
     2341 ttcaatgccc cgcttttggc atggacgtta aggtttttta agtcggcatt accaccgttg
     2401 tttttgatgc tgatgtgttt tccgttgatt gaattgcgtt gttttccgtc accaagctga
     2461 ataccgttgc cggcaaccaa cgtaatatct cctgaagatg aagtgatatt ggtattgtct
     2521 gctttcagac ggcctttacc aaccgatcct gcattgacat cggccttggc tgtcagggta
     2581 ttgtgaccgg taaagtcggc attaccgttg gccaataagg atacatgacc gtctgcagaa
     2641 acagcatgaa ggccgtctga aacgatattg ccctgcaatg cggtggtttc gagagccttg
     2701 gctgcgttca gcttggtatt gcgaagctga atattgcctt tggctgcctg aatgtgcaga
     2761 ttacccgagt tggtacgcag attggtattg gtaacattca gcgagcctgc ctccacaccc
     2821 atgtcttttg aggcagtgag ggttttactg gtgccggtaa tatgggcagc gttatccgat
     2881 ttcaaatgga tgctggcggc agacaaatct ttatcaacat tcaaattcag atctttacct
     2941 gtatgaacat acagattgcc gggagtattc agattggtag ttttggcagt aatgttatcg
     3001 tctgccagta aagcaagctg cttgccgccc ttgatactgc ctccgtttaa gcggatatcg
     3061 gaggtaactg ttgaagcttc caaagaaagc ggtttgcctg cttcgatgtg tgcggtgtct
     3121 ttggcatcta ttacggcgga actgctgatg gtgccgttgg ataatacggt aacatctgcc
     3181 ccggtaatgc gtgtgttatt gcctaattcg gcgttgcctt tgctggaact gtatacggta
     3241 gtgccagtct gaatactggc ctccttgatg acggtacggc cgccggtcga cagattggtc
     3301 gaacctttag cattgttcac atttgctttg ctttcgataa ccaaattacg gccggcattt
     3361 aataccgtag cagcagggcg gttgccgttg ttttgaacga ccgctccgtt acgcaaaccg
     3421 atgtcctccc cggtatcaat aaccaataag cctttgctct ctatccggcc gccattggag
     3481 ataaatgcgc ctgctgcgcc tgtttcggtg gtttcgatgg agagataagt cggtgatgct
     3541 tcagtgctgt cggcagtggt ggcgatgcgg ccgctgtttt caatgcggcc tgacgaagtc
     3601 acaatcaatt gcttggccgc ttcgagtgtg ccggcatttt tgacgcctac gcctttttca
     3661 ttggcaatca gtgtgatgct gtcggcgtac ataccgccca gtgcggcagt atcaagggca
     3721 atagtcggtt tcgtacccgc tgccgtacct gcactgattt cgccgctggc gtaatctact
     3781 ttctgaggac cggtagaaac cgccaggttt ttaccctgta atttcccctg caaagcaact
     3841 gcacgagcaa gtatctcggt gtagtcggct ccgcctttat cattccaacc tgctgctcct
     3901 acggtcaatg tgccttgacg cacatcaaat cctgtcagtg caccgtcttt gccgatttgg
     3961 ggcgcaccgg tagttaagat gccccgaccg acatttttaa agccgccgcc attaacggta
     4021 atgccgttgg ggttggcaat aatcacgtcg gccttttggc cgcctacggt aacgatgccg
     4081 ttgagtttgc tagccgtacc gcgtacctcg ttcaaaatca attgcgcacc gcctttgacc
     4141 agaaacggat tattgttacg gtcgttgttt aacactgccc ctttgttgtc aacatcaaac
     4201 tgcgtatagc ggttgtggct caatccgcgt ccattcggag tttggatatt caccaagggg
     4261 gcaccagtgt tggttttaag gataacgacc tgctggtttt taggtgctga tttgtcggtg
     4321 gtaatttggg catgggcagg caataccata ctcagggaaa ccaaagagca gaccaaggtt
     4381 ttaagggtgg ttttgagttt gccgcaaagg tcgcctgaag ttttcagtga aacagaaacc
     4441 gaactgcctg cctgtttacc tttgccctgg ctgttggcag tttcggctac tgcaaccatg
     4501 gtgctgtgct ttttactaaa gataatgcga tgtaaacctt tattcatgtc tattccattt
     4561 tgaagatgaa cgtactgcgc gccaagtacg taggtaaagt ttgacggtct gaggataagg
     4621 aaagaccgtc taaatatcag taaaaaaatt cagaggttag aaactgtaat tcaagttgaa
     4681 gccgtaaacg gtgttggtcg tctgaaagcc tttgggttta tgaagcggct tgccggcaaa
     4741 cagatcataa gcaaacatac cgcctacttt atgccctcct ctgaagccga ccactgcacc
     4801 catcagctgc ttgcccgata catattgtgc actttcgcca gatacgcggc catagtccgc
     4861 accgagatag aactgatggt tcggatgaaa ataccaagtt aaagtattct gccagtagaa
     4921 acctcgctct ccgaaaagac tctgctcccc atcaaatccg cgaacggtgt agcggctgcc
     4981 gattgacaat ttatcttggg caaccaacgg cgttttgttc cattgagctt gaatggcggt
     5041 tgcgtagaaa aactgctgtt tgcctaaaat aaatggggcg gctgcgtcca aaccggcagt
     5101 aatgattttc atacgagatg tacctggaag aatatcgccg ccgttttctt ccggtgcagg
     5161 catactttgg cgcatgccgg tcccgcgttt gtaagacaac ttgccgtcaa gctgccaacg
     5221 gttgaggtaa gcacggtggc gcaattcggc ttcccagcct gcagagcggc ggcgttgcac
     5281 ttcgatttcg gcatcgtcga tgtatttata ggtttggcgt gtccataatt tcattccgac
     5341 tgaagtttta tgaagtctgt tacgccaaag catgcgctcg gcggccaggc tgctctgata
     5401 ttgtttgccg ttgtaatcgt aattgacgga atagccttcg gttgcttcgt ggtaacgatg
     5461 tccattgtga ttaaaagaaa acagccattt ttttacgggc accgaataat gcacgctgta
     5521 acttctggat ccgctttcag tttccgtacc ggtggcatca gtcaagtccg ttttgtgcac
     5581 caaaccgcgt ccatatgaaa cataaaacaa atcgcttaag cccaaagggt tatcgaacga
     5641 taaagcgaca tttccttgat atttgccggt cgttttgccg cccgcatcat ctataccgat
     5701 actgaaccgt atgggtttat tctgctgcca tttgatctgt aaatcgcttt tgccttcttc
     5761 ttcggacggt ataatctgaa tatctgtttt aacactcgac aaacgacgca ggttttccaa
     5821 gccctgctct acatcgcgaa gattgagaat tttgttccta tataagggaa atttgttatt
     5881 gaatgtacta atactgcctt cggcagactt cccatcccgt ttttcttcat agcggatatc
     5941 ccctatttcg cctgctgata cccgtaattt cagaattccc gaatccatat tctgtggttg
     6001 gataatagct tgggaagtga ggtagccacg cacgatcagt atctgttgcg cagctttttg
     6061 tagcctgctc aaattattgg aacctaaaca catcccagtt ttaaaagctg tttctttcat
     6121 gagcacagaa ggaagaaaag aaaatttgcg caccgtctta tcatctaaac taatgtaatt
     6181 tacccgagta cacggtgttt catcttcact caggacataa ttgttcttct ccaatggttg
     6241 ctcgaaacgg acatttgcat cagttaacaa ttcagcatct atgtgctgct gacgctgcat
     6301 ggaacggata agttctgcat cgttttcatc ggcagctaag gttttaaggg gtatgacagc
     6361 caggataacc aacagacatg gagcaggaaa aaatttcatg acatcaatat tattttagca
     6421 atatttacta ttttgtcata aatttaaaag tatttacagt tatagaatga gacctttgca
     6481 aaattcccca aaattcccac caagacattt aggggatttt ggggaatttt gcaaaggtct
     6541 cggacagtat tttgaacgca gtgcgcgtaa atccgtatgg aaaccatgaa atcccgccac
     6601 agccgccaga catgccaagc cgcattctga tatttctgtt tgcaggataa caggcagctt
     6661 tttctttaag cccaaagaca ggttttgcag atggggcata gatttccttt ttgaaaaata
     6721 gggattagga agttggatgt attttagaaa ggccgcctga aaaggtttca gacgaccttt
     6781 tgcgactagc tgctatttta tttaaagctt ttctctaaca aacgagctaa tatttttcct
     6841 gtaataaaac agataaaaaa cagcatccaa tacgtcagat tggaaaaatc ggtcgtatag
     6901 agaatcaaca tataaagaag cagcatgatg ccgagtgcga tgaattgata atgtttggca
     6961 aacatcatga cctcctcaac tattaaggca aaccgcctga atattctcgt tcaatcgttt
     7021 cggcaatttc cctataacgt cgataccatg accagtcgaa attttcaatg gcatggctcg
     7081 caaacgtacc aaattcaggc atccctatgc ggctacctgc taaagctccg attgtagctc
     7141 cccaatcagg cggattactg aacgtattgt ttggcaatcc taaagattta tcaggatttc
     7201 ccgtatcgtt aagccctgca gattcgcaaa tttcgcaata atatgaactt tgttgcgcat
     7261 tacctgagcc tccgacccaa gtcatttcat gaacacatat agtggattaa atttaaatca
     7321 ggacaaggcg acgaagccgc agacagtaca aatggtacgg caaggcgagg caacgctgta
     7381 ctggtttttg ttaatccact ataaaactct cattttgaaa ctccttgtat cgttaatcaa
     7441 acaatcaaaa gggcagatgc cctatccttg cttttacag
//
LOCUS       DAXHSF010000102         7333 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.102, whole genome shotgun sequence.
ACCESSION   DAXHSF010000102 DAXHSF010000000
VERSION     DAXHSF010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7333
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.102"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            200..412
                     /locus_tag="ACFM8S_001458"
     CDS             200..412
                     /locus_tag="ACFM8S_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFC6314521.1"
                     /translation="METLILDIGGMSCGGCVKSVTRILEGVKGVASVEVSLENKSATV
                     GYDPAQTDAGALIEAVEDGGYDAALK"
     gene            560..2143
                     /locus_tag="ACFM8S_001459"
     CDS             560..2143
                     /locus_tag="ACFM8S_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HFC6314522.1"
                     /translation="MPNKKKKAAFARAATENGVPDVSNIKNSYDDLMYESKAFSQTAI
                     NALEARARMMGLKPAAAAKAKVLELGCSMGGNIITQALYYPDAEFVGIDLSGRQVAQG
                     NAIIEKMGLKNVRLEEKDILTIDESFGKFDYIIVHGIWSWVPDAVKDKIFSICRNNLT
                     EHGIAYISYNVYPGWKRQEQLREIMYFAGRDVLEEPLEARTRKGLDALKALAEILEND
                     KGLGGGGKLPAIQKILNHNTYYVAHEYMEIFNDPIYVNGFIEWANRHRLAYIGDTDLY
                     VSFVSWMAEHTRERILALAGGDYIAKEFYSDILSDRQFRRSLLCREEVGDNIRRDESV
                     AVEVIESLNFRPARGETINFDENDTLLSGIRDVMKTGEPFKTEDVAENLARRFPGLEF
                     DRMKINSQLLLQTILGRFSVSSDNAGKPFFEDHKTYVPARFTNYAAAFVEHGAEAFVR
                     PANRYNESTPSFGYGHLYIMRQLSRPTSKQAVIDAVAENLNIVSTTPDGLTFHPPAEV
                     YVEEILADLADRHFLVSAD"
     gene            2225..3322
                     /locus_tag="ACFM8S_001460"
     CDS             2225..3322
                     /locus_tag="ACFM8S_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFC6314523.1"
                     /translation="MNQTFTLPDTRPYPQNQIKNHLLLNAYQLAHNSSQASRKLSSGQ
                     LQTEIRGMLEQNHYINLSLALTMSPDAGTYAALLSSVNAVLDCEKEGEVQWFALPVVL
                     VSGCKKERAIEMKLPTEGLFACLQNYPHLRALTQETQWLPYLVHSSDLSAVAPDEWWR
                     AKQNTEAAAQHLRRFAPRPLLLPEGQSVHVVYALGFGSGKVQTALGQNLLQAGLPLMQ
                     VWQENLASEGVTLFANPLSPDSPVRALSDGSHTRQRMAMDVFAANAIRAVRMQSPRVG
                     VVAAAKAGGQVLFGFNATDGAFEVVPQVFSWQLSFTDNIGVVQQNFLDLMAECRVEHV
                     YLLHNPLGEQENIPSYAEALKREGHNPFFSA"
     gene            3436..4551
                     /locus_tag="ACFM8S_001461"
     CDS             3436..4551
                     /locus_tag="ACFM8S_001461"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFC6314524.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLEEKCLQVTGPFGQKEVMRYFQKEQLALFEVADLVGLGKIPLEKR
                     NPLQIQTRGIGELIRHLISQEIKEIYIGVGGTASNDGGIGIAAGLGYQFYDEDGNTLP
                     ACGQALLNLASVSTENRYEIPEDVHIRILADVVSPLCGHQGATYTFGKQKGLDSTMFE
                     AVDQAIQDFYEKVSPATLKLKGAGAGGGIAGGLCAFAQASIVSGIDTCLDLIDFDKKV
                     SDVDLVIVGEGRLDRQSLAGKAPIGVAKRTPVGVPVVAICGSLVEDLPSLPFENIQAA
                     FSILEKSEPLEDSLKNASLYLEHTASNIGHLLNMPKI"
     gene            4676..5131
                     /locus_tag="ACFM8S_001462"
     CDS             4676..5131
                     /locus_tag="ACFM8S_001462"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFC6314525.1"
                     /translation="MKKPKILFVCLGNICRSPMAEYILRRRAAEAGIPLEADSAGTSG
                     WHDGEDMHRETAKILKKHGIDASGFTSRKIRQSDAVAFDCIIAMDGKNLSELEKTFGR
                     RSEKIFKLTDLIPESGYDHVPDPWYTGDFEETFRLADAGCRALLEKISK"
     gene            5202..5678
                     /locus_tag="ACFM8S_001463"
     CDS             5202..5678
                     /locus_tag="ACFM8S_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFC6314526.1"
                     /translation="MKTNFKQKIIEQARSEGLQVTALREQVLDIVLQQSGVIKAYNVL
                     SQMQQQSEGVLAPPTAYRALDFWAEQGVLHKVAAVNGYILCSHAQHECDDHCHDHEEA
                     EAHHSAFILVCTECGTADEQTLSHEWAALRAGVAESGFALKEEHVVLTGICKKCQK"
     gene            5666..6592
                     /locus_tag="ACFM8S_001464"
     CDS             5666..6592
                     /locus_tag="ACFM8S_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFC6314527.1"
                     /translation="MSEMKKTKVHLISGFLGTGKTTALKSLMAQKDPNEKWVIIVNEF
                     GEIGIDGAVLSDNGIPVAEIAGGCLCCTAGPQMGVTVQKMLRDAKPDRLMIEASGLAH
                     AASVIDELKAKPLDSLLEIGAVFTVVNPRQFINPDYAQQALYKDQIGICDVLVASKTD
                     LCTTEQLAEFHDKAAKLFPPKAKVVEVQNAQLDIRWLDIPVIEKSRYRLKALPDNTMG
                     FQSQGFTFPAGRDFDGEKLTNFFNDLPKMTEGLVRAKGVFQVLGTWVWLNWVDGQWGA
                     NQVSWRRDSRFELIAKSFDADLIEQKLKDALE"
     gene            6657..7214
                     /locus_tag="ACFM8S_001465"
     CDS             6657..7214
                     /locus_tag="ACFM8S_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC6314528.1"
                     /translation="MKPKFKTVLTALLLAVSLPSMAATHVLMETDMGNIRLVLDESKA
                     PKTVANFVRYARKGFYDNTIFHRVIGGFVIQGGGLTEDLAQKASDKAVANESGNGLKN
                     TVGTIAMARTTDPDSATSQFFINLADNDSLNYKNGQYGYTVFGRVESGMNAVSKIARV
                     KTATRGFYQNVPVQPVKIRRVVVGQ"
BASE COUNT         1925 a         1792 c         1920 g         1696 t
ORIGIN      
        1 tgcgccgatt gccaaggcga tgatgctcaa ccagggtttt tgcgaagcgt tcattgttgt
       61 cgtcctgatg ggaattgccg ccattatgcg caagcgtccg tctttttcca aaccgccgca
      121 ccgtttccgc gtttgcccaa gttcacgccc cgacttacaa taacgcccgt cagattgcag
      181 ttccaacgaa aggaaaatga tggaaaccct tatcctcgac atcggcggca tgagctgcgg
      241 cggctgcgtc aaaagcgtta cccggatatt ggaaggcgta aaaggcgtgg caagcgtcga
      301 agtcagtctt gaaaacaaaa gtgcgaccgt cggatacgac cccgcgcaaa ccgatgcagg
      361 ggcgttgatt gaagccgttg aagacggcgg ctatgatgcc gcgttgaaat aaagcggcaa
      421 aaatgccgtc tgaagccttc gcgcctcccc gatgccgtct gaagtacgct ccgcgccttc
      481 agatggcatt tgatttagtt gaacgggtag gatgaattgg ttaaacttgc gggcaatagt
      541 gccttcagac aaacaaaata tgccgaacaa aaagaaaaaa gccgcctttg cgcgtgctgc
      601 aacggagaac ggtgtgccgg acgtgtccaa catcaaaaat tcatatgatg atttgatgta
      661 tgaatccaaa gcattttccc aaaccgccat caacgccttg gaggctcgcg cccgcatgat
      721 gggcctgaag cctgcagcag cggcaaaagc caaggtcttg gagctgggct gttcgatggg
      781 cggcaatatc atcacgcagg ctctttatta cccggatgcg gaatttgtcg gtatcgactt
      841 gtccggcagg caggttgcgc agggcaacgc catcattgaa aaaatgggct tgaaaaatgt
      901 gcgtctggaa gaaaaagata ttttgaccat cgatgagtca ttcgggaagt ttgactatat
      961 catcgtccac ggcatttggt cgtgggtgcc tgacgcagtt aaagacaaaa ttttttcgat
     1021 ttgccggaac aacctgaccg aacacggcat tgcctatatt tcatacaatg tttaccccgg
     1081 ctggaaacgg caggaacagt tgcgcgaaat tatgtacttt gccggcaggg atgtgcttga
     1141 agaacccttg gaagcgcgaa cgcggaaagg cttggacgcg ctcaaggcgc tggcggaaat
     1201 tttggaaaac gacaagggct tgggcggcgg cggcaaactt ccggcgattc aaaaaatatt
     1261 gaatcacaat acttattatg tcgcacacga atatatggaa atattcaacg acccgattta
     1321 cgtcaacggc ttcatcgaat gggctaaccg ccaccggctg gcgtatatcg gggataccga
     1381 tttgtacgtg tcttttgttt cttggatggc ggagcatacg cgggagcgga ttttggcatt
     1441 ggcagggggc gattatattg ccaaagaatt ttacagcgat attttatctg accgccaatt
     1501 ccgccgttcg cttttatgcc gtgaggaagt cggggacaat atcaggcgtg atgagtcggt
     1561 tgccgtcgaa gtgatagaaa gtttgaattt ccgcccggca agaggggaaa cgatcaactt
     1621 tgatgaaaac gataccctgc tttcgggcat acgcgatgtg atgaaaaccg gagagccgtt
     1681 taaaacggaa gatgttgcgg aaaatcttgc ccgccgattt cccggtctgg aatttgaccg
     1741 catgaaaatc aattcccagc ttttattgca aaccattctc gggcgttttt ctgtttcatc
     1801 ggacaatgcg ggcaaaccat tttttgaaga ccataaaacc tatgtgccgg cgcgttttac
     1861 aaactatgcc gccgcctttg tggaacacgg cgcggaagcg tttgtccggc ctgccaaccg
     1921 ttacaacgaa agcacgccct cattcgggta cgggcatttg tacattatgc ggcaattatc
     1981 gcggccgacg agcaaacagg cggtgataga tgcggttgcc gaaaacttga acatcgtcag
     2041 caccacgccc gacggtttga cattccatcc gcctgccgaa gtgtatgtgg aagaaatatt
     2101 ggcagacttg gcagacaggc attttctcgt ttcggcggat tgacgggcaa gtcccgacgg
     2161 caatccgtcc gatatatttt cagacaggaa agacacacac tgcgaaaagc ggtacacgac
     2221 acatatgaac cagacattta ccttgcccga tacgcgcccg tatccccaaa atcagattaa
     2281 aaaccacctg ctgctcaatg cctaccagtt ggcgcacaat tcttcccagg cttcgcgcaa
     2341 actctcgtcc ggccaacttc aaaccgaaat cagggggatg cttgagcaaa accactatat
     2401 caacctttcc ctcgcgctga cgatgtcgcc cgatgccgga acttatgccg cgctgctttc
     2461 cagtgtgaac gcggtactcg attgcgagaa agaaggcgaa gtgcagtggt tcgccctgcc
     2521 ggtcgtgctg gtgtccggct gcaagaaaga acgggcaatc gagatgaagc tgccgacgga
     2581 gggattgttt gcctgtttgc aaaactatcc gcacctgcgc gcgttgacgc aagagacgca
     2641 atggctgcct tatcttgtac attcctccga tttgagtgcg gtcgcgccgg atgagtggtg
     2701 gcgtgccaaa caaaataccg aagcggcggc gcaacactta cgccgtttcg ccccgcgccc
     2761 tttgcttttg cccgaagggc agtccgtcca cgtcgtttac gcgctgggtt tcggcagcgg
     2821 caaagttcag acggcattgg gtcagaacct tcttcaggca ggtctgccct tgatgcaggt
     2881 atggcaggaa aatcttgcat cggaaggcgt tacgctgttt gccaatccgc tttccccgga
     2941 ttctccggta cgcgcgcttt cagacggcag ccacacgcgc cagcgtatgg cgatggatgt
     3001 gtttgcggca aacgcgatac gcgccgtccg tatgcagagt ccgcgcgtcg gcgtggtcgc
     3061 tgcggcaaag gcgggcggac aggttttatt cgggtttaat gcgaccgacg gcgcgtttga
     3121 agtcgtgccg caggtgtttt cgtggcagct ttcctttacc gacaatattg gcgttgttca
     3181 gcaaaatttt ttggatttaa tggcagaatg ccgcgtggaa cacgtttacc tgttgcacaa
     3241 tcctttgggc gaacaggaaa acatcccgag ctatgcggaa gcattgaaac gggaagggca
     3301 caatccgttt ttcagcgcat agtgatttgt agtagtgata ggctttctca tttctataat
     3361 aaatcaaaat aaagagcaca acactttttc attctgtgtt gtgccttgag tgaaacgaaa
     3421 ggatgaatta taaatatgaa gattgtaatt gcaccggatt cgtttaagga aagcttgaca
     3481 gctcaacagg tagctgaagc aataaaaaga ggcttccaac aatcgatagc agatgtggaa
     3541 tgtctcctct gtcctgtcgg tgatggggga gaaggcactg tagatgctat ccgacattct
     3601 cttgacctag aagaaaaatg tcttcaagtg acaggacctt ttggacaaaa agaagtcatg
     3661 cgctattttc aaaaagaaca actagcccta ttcgaagttg ctgatttggt tggtcttgga
     3721 aaaatcccgc tagagaaacg aaatccatta caaatccaaa ctcgtggtat tggagaattg
     3781 attcgccacc tcattagtca agaaattaaa gaaatctata ttggcgttgg cggtacggcc
     3841 agtaatgatg gaggtattgg gattgctgct ggtttaggtt atcaatttta tgatgaggat
     3901 ggaaatacct tgcccgcttg cggtcaagcc ttactaaacc tagcttctgt ttcaacagaa
     3961 aatcgctatg aaattcctga agatgttcac attcgtattt tagcagatgt tgtgagtccc
     4021 ttatgtggac accaaggtgc gacctatacg tttggcaagc aaaaagggtt ggattctact
     4081 atgtttgagg ccgtagatca ggcaatacaa gatttttatg aaaaagtctc ccctgcaaca
     4141 ttaaaactta aaggagcagg agctggtgga ggcatcgctg gtggtttgtg cgcctttgct
     4201 caggcaagta tcgtatctgg aattgacacc tgcttggact tgattgactt tgataagaaa
     4261 gtgtcagatg ttgacttggt tatcgttggt gaaggaagac tagatcgtca aagtttagca
     4321 gggaaagcgc ctattggtgt agcaaaaaga acccctgtcg gagttcctgt tgtcgctatt
     4381 tgtggcagtc tcgttgaaga tttgccttcc ctgccatttg aaaatatcca agctgccttt
     4441 tctattttgg agaaaagtga acctttagaa gatagtttga aaaatgccag tctctatttg
     4501 gagcatacgg cttctaatat tggacactta ttaaatatgc ctaagattta gccaaaccat
     4561 ttcttccaga tggatgtttt ggctggttct gccttatgcg gttacgatta accgaccgat
     4621 gccgtctgaa agggtttccg cttcagacgg catcgcgccg gaacagggag gggatatgaa
     4681 aaaaccgaaa atcctttttg tctgcctcgg caacatctgc cgttcgccga tggcggaata
     4741 cattttgcgc cgccgcgccg ccgaagcggg cattcccctt gaagcggaca gcgcggggac
     4801 atcgggctgg cacgacgggg aagatatgca ccgcgagacg gcaaagatat tgaaaaaaca
     4861 cggtatcgat gcttcaggct ttaccagccg caaaatccgc caaagcgatg cggtggcgtt
     4921 tgactgcatc atcgcgatgg acggcaagaa tttgtccgaa ttggaaaaaa ccttcggcag
     4981 gcggtcggaa aaaatattca agctgaccga cctgataccc gaaagcggtt acgaccatgt
     5041 ccccgatccg tggtacacgg gcgattttga agaaaccttc aggcttgcgg atgcgggctg
     5101 tcgggcattg ttggaaaaga tttccaaata aagcaattga atacaaatat aaaaccgcct
     5161 cctgccgtat cggttgttca gacggcataa acagagaatt tatgaaaaca aatttcaaac
     5221 agaaaattat cgaacaggca cgcagcgaag gcttgcaggt aaccgctttg cgcgagcagg
     5281 tattagatat tgtcttgcag caaagcggcg tgattaaagc ctacaacgtc ttgtcgcaga
     5341 tgcagcagca aagcgagggc gtgcttgcac cgcctaccgc ctaccgcgcc cttgattttt
     5401 gggcggagca gggcgttttg cacaaagtgg cggcggtcaa cggctatatt ttgtgcagcc
     5461 acgcgcagca cgagtgcgac gaccattgcc acgaccacga agaagccgaa gcgcaccaca
     5521 gcgcgtttat tttggtctgc accgaatgcg gcacggcgga cgagcaaacc ctcagccacg
     5581 agtgggcggc actgcgcgca ggcgttgccg aaagcggctt tgcgctgaaa gaagaacacg
     5641 ttgttttaac tgggatttgt aaaaaatgtc agaaatgaaa aaaaccaaag tccacctgat
     5701 ttcaggtttt ctgggaacag gcaaaaccac cgcgctcaaa agcctgatgg cgcaaaaaga
     5761 cccgaacgaa aaatgggtca tcatcgtcaa cgagttcggc gaaatcggta ttgacggcgc
     5821 ggtattgagc gacaacggca tccctgtggc agaaatcgcc ggcggctgtt tgtgttgcac
     5881 cgccggcccg caaatgggcg taaccgtgca gaaaatgctg cgcgacgcca agcccgaccg
     5941 cctgatgatt gaggcaagcg gactggcgca cgcagccagc gtcatcgacg aattgaaagc
     6001 caaaccgctg gacagccttt tggaaatcgg cgccgtcttt accgtcgtca atccgcgcca
     6061 gttcatcaac cccgattacg cgcaacaggc gttgtataaa gaccaaatcg gcatctgcga
     6121 cgtattggtc gccagcaaaa ccgatttatg caccaccgaa cagcttgccg aatttcacga
     6181 caaagcggca aaactgttcc cgcccaaagc caaagtggtc gaagtccaaa acgcacaact
     6241 cgacatccgg tggctcgata ttcccgtcat cgaaaaatca cgctaccgcc tcaaagccct
     6301 gccggacaac accatgggct tccagtcgca aggtttcaca ttccccgccg gacgcgattt
     6361 cgacggcgaa aagctgacca actttttcaa cgacctgccc aaaatgaccg aaggactcgt
     6421 ccgcgccaaa ggcgtgtttc aagtattggg aacgtgggtg tggctcaact gggtagacgg
     6481 acagtggggc gcgaaccaag tctcatggcg gcgcgattcg cgttttgaat tgattgccaa
     6541 atcgtttgat gcggatttaa tcgaacaaaa gctcaaagac gcattggaat agcgttcacg
     6601 cgccttcaga cggcatatga aatgaaaatg ccgtctgaag gtctgaggag gacgggatga
     6661 aacccaaatt caaaaccgtt ttaaccgcgc tgcttttggc ggtttccctg ccgtctatgg
     6721 cggcaaccca tgttttgatg gaaaccgata tgggcaatat ccgtttggtt ttggacgaat
     6781 ccaaagcacc caaaaccgtt gccaatttcg tgcgctatgc ccgaaaaggc ttttacgaca
     6841 atacgatttt tcaccgcgtc atcggcggct tcgttatcca aggcggcgga ttgaccgagg
     6901 acttggcaca aaaggcaagc gataaggccg ttgccaacga atccggcaac ggcttgaaaa
     6961 acactgtcgg caccatcgcc atggcgcgga cgacagaccc cgattccgcc accagccaat
     7021 tctttatcaa tctggcggac aatgattcac tcaactacaa aaacggacaa tacggctata
     7081 ccgttttcgg cagggtcgaa agcggtatga acgccgtttc caaaatcgcc cgcgtcaaaa
     7141 ccgccacgcg cggcttttat caaaacgtac ccgtacagcc cgtcaaaatc cgtcgcgttg
     7201 ttgtcgggca gtaacacgca gacagacgtt cagacggcgt cgcccgtttc ccaaaaaacg
     7261 ccgtttaaat taaaaaaata ttttaaaaca gacagttgat attgacaaat tcaaaccgag
     7321 gattttaaaa tgc
//
LOCUS       DAXHSF010000103         7170 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.103, whole genome shotgun sequence.
ACCESSION   DAXHSF010000103 DAXHSF010000000
VERSION     DAXHSF010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7170
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.103"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(1..404)
                     /locus_tag="ACFM8S_001466"
                     /pseudo
     CDS             complement(1..404)
                     /locus_tag="ACFM8S_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1375..2145)
                     /gene="xth"
                     /locus_tag="ACFM8S_001467"
     CDS             complement(1375..2145)
                     /gene="xth"
                     /locus_tag="ACFM8S_001467"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFC6314529.1"
                     /translation="MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPA
                     AALQMMGWHCVWSGQKTYNGVAIVSRNAPEDVHIGLPALPDDPQRRVIAATVGGVRVI
                     NVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMTRYGKLVLLGDFNIAPADADCYDPE
                     KWHEKIHCSSVERQWFKNLLDLGLTDSLRQVHPEGAFYTWFDYRGAMFQRKLGLRIDH
                     ILVSPAMAAALKDVRVDLETRALERPSDHAPVTAEFDW"
     gene            complement(2199..2486)
                     /locus_tag="ACFM8S_001468"
     CDS             complement(2199..2486)
                     /locus_tag="ACFM8S_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFC6314530.1"
                     /translation="MNTILLHTILKLMAHPERMAILIQLLDSERNIAELAKSLSLPAT
                     AVSGHLNRLRAGGLVDFTRYHRIIEYRLISEDAATILRTIRDLENKRAVWC"
     gene            2543..2821
                     /locus_tag="ACFM8S_001469"
     CDS             2543..2821
                     /locus_tag="ACFM8S_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314531.1"
                     /translation="MIVIIFINRIINQIFMDDCTRLYVPFPTFFANTRKGGLSDGIKL
                     PPLPCLTLLPNGIIRIVQKMPYNRRTSCPADASVPSGKQNTCLKRCFF"
     gene            complement(2954..3835)
                     /gene="nadC"
                     /locus_tag="ACFM8S_001470"
     CDS             complement(2954..3835)
                     /gene="nadC"
                     /locus_tag="ACFM8S_001470"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFC6314532.1"
                     /translation="MPSENTLFSLPDTLLRPIVEQALSEDLGRRGDITSAAVIAPDKT
                     AKLFLVSREDGVIAGMDLACLAFQTMDPSVRFQAEIQDGQAVRAGQTLAVVEGNACAL
                     LAAERTALNYLTHLSGIATATARAVAEVAEYGTDIVCSRKTIPLLRVLQKYAVRAGGG
                     VNHRMGLDDAVLIKDNHLAYCGSIAQAVRQAKQAVGALTCVEIEVDTLAQLDEAIAAG
                     AERILLDNMDDETLKEAANRCHTQTAHPHTVYCEASGGIGFDRLKRVAQTGVDGIALG
                     YLTHSSRSLDIGLDFLA"
     gene            complement(3911..4849)
                     /locus_tag="ACFM8S_001471"
     CDS             complement(3911..4849)
                     /locus_tag="ACFM8S_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314533.1"
                     /translation="MDAYPEAEAPPQSIVELVPVLIAVTDGGLRVLTVAQGTLLPNGP
                     LSPLRNSLQAGVKLWVAKQTSQPMGYVEQLYTFVDTHRRNEHGMPVLYVSYLGLVREA
                     ADSILHPDAKWQDCYGYFPWEDLRTDGGQRDAVVSRLRIWANSADTEEVRQMRLKRIH
                     LCWGVEPENWSEEYVLQRYEMLYESGLIVEAAEPQANFDFALTGQPMRHDHRRVLATA
                     LSRLRAKIKYRPVIFELMPPEFTLLQLQNSVEAISGRLLHKQNFRRQIQQQNLIEPSD
                     TGVSGSKGRPAQLCRFRDDVLPDRLISDIGLPLGSR"
     gene            complement(5102..5929)
                     /gene="fghA"
                     /locus_tag="ACFM8S_001472"
     CDS             complement(5102..5929)
                     /gene="fghA"
                     /locus_tag="ACFM8S_001472"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFC6314534.1"
                     /translation="MKLIEQHQIFGGSQQVWAHHAQTLQCEMKFAVYLPNNPENRPLG
                     VIYWLSGLTCTEQNFITKSGFQRYAAEHQVIVVASDTSPRGEQVPNDDAYDLGQGAGF
                     YLNATEQPWAANYQMYDYILNELPRLIEKHFPTNGKRSIMGHSMGGHGALVLALRNQE
                     RYQSVSAFSPILSPSLVPWGEKAFTAYLGKDREKWQQYDANSLIQQGYKVQGMRIDQG
                     LEDEFLPTQLRTEDFIETCRAANQPADVRFHKGYDHSYYFIAGFIGEHIAYHAAFLK"
     gene            complement(5938..7047)
                     /locus_tag="ACFM8S_001473"
     CDS             complement(5938..7047)
                     /locus_tag="ACFM8S_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase [NAD(P)+] activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HFC6314535.1"
                     /translation="MKSRAAVAFAPNQPLQIVEIDVEMPRKGEVLIRNTHTGVCHTDA
                     FTLSGSDPEGVFPVVLGHEGAGVVVAVGEGVSSVKPGDHVIPLYTAECGECEFCCSGK
                     TNLCVSVRDTQGKGLMPDGTTRFSYQGQPIYHYMGCSTFSEYSVVAEVSLAKINPEAN
                     HEQVCLLGCGVTTGIGAVHNTAKVQEGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPSKFELAKQFGATDCLNPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRQALES
                     AHRGWGQSIIIGVAGAGQEISTRPFQLVTGRVWKGSAFGGVKGRSELPKMVEDSMKGD
                     IELEPFVTHTMTLDQINKAFDLMHEGKSIRAVIHY"
BASE COUNT         1690 a         1826 c         1789 g         1865 t
ORIGIN      
        1 tacggcttac cctcggacgg caggcgttgg tagaaatccc gaataagcgg ttcaaaacgt
       61 gtcgctgcca cggtagccat atacagtgcc ttacgcaccg cagaccttcc gccaaagcag
      121 cggcttttga atttggtttc cccgctatcc ctcgggtgcg gggcaatgcc ggccaaactc
      181 gctatccgtt tgtgcgacag ccgccccaat tcgggcagca tcgccatcag cgtagccgtc
      241 gttatcgaac cgatgccttt gatttgctcc gccacttggg ctttgccgtc aaaatgcgtg
      301 tgggtgtggt cgtcgatttg tttgtccaat tcgtcaatca gccggtcaaa atgggcaatc
      361 agttgtttga cgcttccgac ttgcgtttca tgaaactgat gcagatatag tggattaaca
      421 aaaatcagga caaggcgacg aagccgcaga cagtacaaat agtacggcaa cgctgtactg
      481 gtttaaattt aatccactat attttgtaat agctgccatt atgcctacta ggatatatac
      541 cttctatata atccttattt aaataaaaac ccttataaaa atctgtgtta tctgtcggga
      601 ttgaaaaaaa gattttatct ttctcatcag gtttatattt gctatcaata tcaaaaagag
      661 aataaggatt acaatgagtt cgacatacta ggtaaaaatt tatatcttgg atcatgaaat
      721 attcgatatt tatcaggatc gaaagatgct aaaaattcat caatatcggt actggatatt
      781 ttgtcatcac taataaacat cgctagagat gggcgacttt gattttccat ttgttgatta
      841 gtaaaggata taaatactaa cttgccattg tcaaaccaca tagatttata tagtggatta
      901 aatttaaacc agtacgtcgt tgcctcgcct tgtcctgatt tttgttaatc cactatagtt
      961 attgcctttg ggagaatcaa ttctgacagc acataacata ggttcatctt tacagtcgtt
     1021 atattgggtt tcatcaaact caactgcata gcacaataaa ggcaatagga aaaaaaggag
     1081 attaatttat tactatacat aaggcagcct gactattcat tctaaaataa atccggtttt
     1141 taatatttga ttctgctccg gattatatac ataataaatt ttactgctcc aaaatctgat
     1201 attaacccta tggatttcat aaatttttca gaaaaatagg ctgtgcttga acaatccgcc
     1261 gactccggct gtttgtcagg gaaaaggcaa ttattttcgg ttagtcagca gcctatccta
     1321 ttgaatcact taaagatatg gatgtttgtc aacgccatat caaaacacgg tcttttacca
     1381 atcgaattct gccgtcaccg gcgcgtggtc gctcggacgc tccagcgcgc gcgtctccaa
     1441 atcaacgcgg acatccttca acgccgccgc catcgcaggc gacaccaaaa tatggtcgat
     1501 acgcaggccc agtttgcgtt ggaacatcgc gccgcgatag tcgaaccagg tatagaacgc
     1561 gccttcggga tggacttggc gcaggctgtc ggtcagtccc aaatccagca ggtttttgaa
     1621 ccactgccgt tcgacggacg aacagtggat tttttcgtgc catttttcag ggtcgtaaca
     1681 gtccgcatcg gcaggcgcga tattgaaatc gcccagtagc accagtttgc cgtagcgggt
     1741 catttcatcg cggacaaatt cggttaacgc ggcaaaccat tgttctttat atttgaattt
     1801 cgggctgtcg agggcttcgc cgttgacgca atagacattg atgacgcgca caccgccgac
     1861 ggttgccgca atcacgcgcc gttgcggatc gtccggcagc gcgggcagcc cgatatgcac
     1921 gtcttccggc gcgttgcggc tgacgattgc cacgccgttg taggttttct gcccgctcca
     1981 aacacagtgc cagcccatca tttgcaaagc ggcggccgga aatttgtcct gatcgagttt
     2041 gagttcctgc aaaaccaaaa tatcgggcgg attgtcggca agcaggtttt gcacctgcgg
     2101 cagccgcaca ttgagcgaat tgacgttcca agtggtgatt ttcataacat ttccgaaaaa
     2161 tgccgtctga aacgttcaga cggcaaaagg aaaggatttt aacaccatac cgcgcgtttg
     2221 ttttccaaat cgcggattgt ccgcagaatc gttgccgcgt cttcggaaat caggcggtat
     2281 tcgataatgc ggtggtaacg cgtaaagtcc accagaccgc ccgccctcag gcggttcaaa
     2341 tggccggaaa cggcggtggc cggcagggat aaggattttg ccagttcggc gatattgcgt
     2401 tcgctgtcca acagttggat cagtatcgcc atacgttcgg gatgcgccat aagtttgagt
     2461 atggtgtgga gcagtattgt gttcatcggt attcctaaaa cctttgccgc ccgccaagcg
     2521 gattgacggg cgacagatat ggatgattgt gattatcttt ataaatcgta ttataaatca
     2581 aatatttatg gatgattgta caagattgta tgttcctttt cccacttttt ttgcaaacac
     2641 gcgtaaaggc gggctttcag acggcataaa actgccgccc ctaccctgcc tcaccctgtt
     2701 gccgaacgga atcatccgca ttgttcaaaa gatgccttac aaccgccgca cgtcttgccc
     2761 tgccgatgcc tccgtgcctt cgggcaaaca aaatacctgc ctgaaacgct gttttttcta
     2821 aagcatcacg accttcacga aaaatgccgt ctgaagaata tttcagacgg catttcctcc
     2881 ttgaggcaat aaccggatta cggttaggcg gttccttgtc tgacatatca gacaactgcc
     2941 gacaccctaa aactcatgcc aagaaatcca aacctatgtc caacgagcgg ctgctgtggg
     3001 tcagatagcc gagggcgatg ccgtccacgc cggtttgcgc cacgcgcttc aggcggtcga
     3061 agccgatgcc gcccgatgct tcgcaataga cggtgtgggg gtgggcggtt tgcgtgtggc
     3121 agcggtttgc cgcttctttc agggtttcgt cgtccatgtt gtccagcaaa atccgttccg
     3181 cgcccgctgc gatggcttcg tccagttgtg ccaacgtgtc cacttcgatt tccacgcagg
     3241 tcaatgctcc gaccgcctgt tttgcctgcc gaacggcttg ggcgatgctg ccgcaatagg
     3301 cgaggtggtt gtctttgatg agcacggcgt cgtccaagcc catgcggtgg ttcacgccgc
     3361 cgcctgccct gacggcgtat ttttgcagga cgcgcagcag ggggatggtt ttgcggctgc
     3421 acacgatgtc tgtaccgtat tcggcgactt cggcaacggc acgcgcggtg gcggtggcga
     3481 tgccgcttaa gtgcgtgagg tagttgagtg cggtgcgttc ggcggcgagc agcgcgcagg
     3541 cgttgccttc gacgacggcc agcgtctgac ctgcgcggac ggcttgtccg tcttggattt
     3601 cggcttggaa acggacggac ggatccatcg tctgaaaggc gagacacgcc aagtccatac
     3661 cggcgataac accgtcttcg cggctgacga ggaagagttt ggcggttttg tcgggggcga
     3721 taacggcggc ggacgtaatg tcgccgcgcc tgcccaagtc ttcgctcaag gcttgttcga
     3781 ctatggggcg caacagggtg tcgggcaggg aaaagagggt gttttcagac ggcatgaggt
     3841 ttcctttgtc gaaaaagatt gatgttgcac cgatggggca aaacttcatt tgatgccgtc
     3901 tgaaaacggg ctaacggctg cccagcggca gtccgatgtc cgaaatcagc ctgtcgggca
     3961 ggacgtcgtc gcggaagcgg caaagctgcg cgggacggcc tttgctgccc gatacgccgg
     4021 tatccgacgg ctcgatgagg ttttgctgct gaatctggcg gcggaagttt tgcttgtgca
     4081 gcaatcttcc gctgatggct tcgacactgt tttgcagttg cagcagcgtg aattcgggcg
     4141 gcatcagctc aaaaatcacg gggcggtatt tgattttggc gcgcaggcgg gacagggcgg
     4201 tcgccaatac gcggcggtgg tcgtggcgca tgggctgccc tgtgagcgcg aaatcgaagt
     4261 ttgcctgcgg ctcggcggct tccactatca ggccgctttc atacagcatt tcatagcgtt
     4321 gcaaaacgta ttcttccgac cagttttccg gttcgacccc ccaacacaaa tgaatgcgct
     4381 tgagccgcat ttgacgcact tcctccgtgt ccgccgagtt tgcccaaatg cgcagtcggc
     4441 tgacgacggc gtcgcgctgt ccgccgtcgg tgcgcaagtc ttcccacggg aaatagccgt
     4501 agcagtcctg ccatttcgca tcgggatgca ggatgctgtc ggctgcctcg cgcaccagcc
     4561 ccaaatagct gacgtacaag acgggcatgc cgtgttcgtt gcggcggtgg gtatcgacaa
     4621 aggtgtaaag ctgttccaca tagcccatag gctgcgaagt ctgcttggca acccacagct
     4681 tcacgcccgc ctgcaaggaa ttgcgcagag gggagagcgg gccgttgggc aggagtgtgc
     4741 cttgggcgac ggttaatacc cgcaggccgc cgtcggtaac ggcaatcaat acgggaacca
     4801 gctcgacgat gctttgcggc ggggcttcgg cttcggggta ggcatccatg ctcggctttc
     4861 tgcgggtggg aaaggcttta ttgtatagtg gattaaattt aaaccagtac agcgttgcct
     4921 cgccttgccg tactatttgt actgtctgcg gcttcgtcgc cttgtcctga tttttgttaa
     4981 tccactataa tccgtagagt cggcttcttc cggctcagac ggcatacgtt tatgccggca
     5041 cttcttaccg ccctcgtgcc ggctatccca aaactgcttt gaacactgaa cgctctttgg
     5101 cttacttcaa aaatgccgcg tgataggcaa tatgctcgcc aataaaaccg gcgatgaagt
     5161 aatagctgtg atcatagcct ttgtggaagc gcacatcggc cggttggttt gccgcacgac
     5221 aggtttcgat aaaatcttcg gtacgcaatt gtgtcggcaa aaactcatct tccaagcctt
     5281 gatcgatgcg cataccttgc actttatagc cttgttgaat gagtgagttc gcatcatatt
     5341 gctgccattt ttcacggtct ttccctaaat aagcagtaaa ggctttttct ccccacggca
     5401 cgaggcttgg cgataaaata ggcgaaaagg cagaaacact ttgataacgt tcctgattcc
     5461 gcagcgccaa taccaatgcg ccgtgtccgc ccattgaatg ccccataatg gaacgtttgc
     5521 cgttggtagg aaagtgtttc tcaatcagac ggggcagctc gttcaaaatg taatcataca
     5581 tttgataatt cgccgcccaa ggctgttcgg tcgcattcaa ataaaagcct gcaccctgtc
     5641 ctaaatcgta agcatcatcg ttcggcactt gctctccgcg agggctggta tcggaggcca
     5701 ccacaattac ttgatgttct gccgcataac gctgaaagcc tgatttggta atgaaatttt
     5761 gttccgtaca cgtcaagccg gaaagccaat aaatcacacc aagcggtcga ttttctggat
     5821 tatttggcaa atagacggca aatttcattt cgcattgcag cgtttgggca tgatgcgccc
     5881 aaacttgttg cgaaccgcca aaaatttgat gttgttcaat cagtttcatc gcatacctta
     5941 gtagtgaata acggcgcgga tcgatttacc ttcgtgcatt aagtcaaagg ctttattgat
     6001 ttgatcgagt gtcattgtgt gggttacaaa cggttctaac tcaatgtcgc ctttcattga
     6061 atcttccacc attttcggca gttcagagcg acctttcaca ccgccaaatg ctgaaccttt
     6121 ccaaacacga cctgttacca actggaacgg acgcgttgaa atttcttgtc ctgcacctgc
     6181 tacgccgatg ataatggatt gaccccaacc acgatgtgca ctttctaatg cctgacgcat
     6241 tacgtttaca ttgccgatac attcaaaggt atggtcaatg ccccatttat taatgtctaa
     6301 caacacatct ttgatcggtt tatcgtaatc gttcgggttc aaacaatccg ttgcaccgaa
     6361 ctgttttgcc aactcaaatt ttgatggatt ggtatcaatg gcgataatgc ggccggcttt
     6421 ggcttgacgc gcaccttgca ccaccgccaa accaatcgcc cccaaaccaa acacggcaac
     6481 agagtcgcct tcttgcactt ttgccgtatt atgtaccgca ccaatacctg tggtaacgcc
     6541 gcagccgagc aaacatactt gttcatggtt ggcttcaggg ttgattttcg ccagtgaaac
     6601 ttcggcaaca acggagtatt cactgaaagt cgaacagccc atatagtgat agattggctg
     6661 accttgataa gaaaaacgcg tcgtgccgtc cggcattaag cctttacctt gtgtatcacg
     6721 cactgagacg cacaagttgg ttttacctga acaacaaaac tcacattcgc cacattcggc
     6781 ggtgtaaagc ggaatcacgt gatcacccgg ttttacgctt gacacacctt cgcccacagc
     6841 aacgaccaca cccgcacctt cgtgtccaag caccacaggg aatacgcctt caggatcgct
     6901 tcctgataac gtaaacgcat cagtatggca cacgccagtg tgggtattgc ggattaacac
     6961 ctcgccttta cgcggcattt ctacgtcgat ttccacaatt tgtaagggtt ggttaggggc
     7021 gaatgccacc gccgcacgag atttgatggt tgaatcggtt tgtttcattt ccattgtctt
     7081 tcctcttaat tcattgacaa agttgcctta cctgagcaac atagcaacag tttacacttt
     7141 agagttaact ctaaagcaag tgttttttac
//
LOCUS       DAXHSF010000104         7124 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.104, whole genome shotgun sequence.
ACCESSION   DAXHSF010000104 DAXHSF010000000
VERSION     DAXHSF010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7124
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.104"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(1..86)
                     /locus_tag="ACFM8S_001474"
                     /pseudo
     CDS             complement(1..86)
                     /locus_tag="ACFM8S_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349130.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type cleavage/methylation
                     domain-containing protein"
     gene            complement(133..330)
                     /locus_tag="ACFM8S_001475"
                     /pseudo
     CDS             complement(133..330)
                     /locus_tag="ACFM8S_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            complement(359..688)
                     /locus_tag="ACFM8S_001476"
     CDS             complement(359..688)
                     /locus_tag="ACFM8S_001476"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC6314536.1"
                     /translation="MGGLIIEDLQEGFGKEAVKGKEITVHYTGWLENGTKFDSSLDRR
                     QPLTITLGVGQVIKGWDEGFGGMKEGGKRKLTIPSEMGYGAHGAGGVIPPHATLIFEV
                     ELLKVYE"
     gene            complement(766..984)
                     /locus_tag="ACFM8S_001477"
     CDS             complement(766..984)
                     /locus_tag="ACFM8S_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HFC6314537.1"
                     /translation="MLKTLTFAALHFSVAFSVTYVLTGSIGVSGAVALVEPLINTVVF
                     YFHEKAWNLYEKNKTVKQTQPFQLHRCS"
     gene            1106..2059
                     /locus_tag="ACFM8S_001478"
     CDS             1106..2059
                     /locus_tag="ACFM8S_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HFC6314538.1"
                     /translation="MNHKKIVVLDADTLPGRVFHFDFPHELAVYGTTGADETAERVRD
                     AHIVITNKVMISADIIAANPQLELIAVSATGVNNVDIGAAKAAGVAVCNVRAYGNESV
                     AEHAFMLMIALMRNLPAYQRDVAAGLWEKSPFFCHYGAPIRDLNGKTLAVFGRGNIGR
                     TLAGYAQAFGMGVVFAEHKHAPDVREGYVSFEDAVRTADVLSLHCPLNAQTENMIGEN
                     ELQQMKPGAVLINCGRGGLVDENALIAALKYGQIGGAGVDVLTEEPPRGGNPLLNARL
                     PNLIVTPHTAWASREALDRLFDILLANIHAFVKGEAQNRVV"
     gene            complement(2135..4192)
                     /gene="metG"
                     /locus_tag="ACFM8S_001479"
     CDS             complement(2135..4192)
                     /gene="metG"
                     /locus_tag="ACFM8S_001479"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFC6314539.1"
                     /translation="MTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGNECH
                     YCCADDTHGTPVMLAAQKQGIAPEDMIAKVREEHLADFTGFFIGYDNYYSTHSPENKQ
                     FSQDIYRALKANGKIESRVIEQLFDPEKQMFLPDRFVKGECPKCHAQDQYGDNCEVCG
                     TTYSPTELINPYSAVSGAKPELRESEHFFFKLGECADFLKAWTSGNNPHDGKPHLQPE
                     ALNKMKEWLGEGEETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNL
                     CDRIGVDFDEYFKADSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLT
                     VDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNSKIEDIDLNLQDFISRVNSD
                     LVGKYVNIAARASGFIAKRFEGRLKDVADSALLAKLAAESDTIAEQYENREYARALRD
                     IMALADAVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTILTAYLAPVLPQTAAN
                     AARFLNLDAITWANTRVTLGEHAINKYEHLMQRVEQKQVDDLIEANKQSIAAAAAPAA
                     EEGKYEKVAEQASFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEKRIIFSGIA
                     ASYPNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATADGKLKLLDVDAGAQPGD
                     KVG"
     gene            complement(4308..6146)
                     /gene="glmS"
                     /locus_tag="ACFM8S_001480"
     CDS             complement(4308..6146)
                     /gene="glmS"
                     /locus_tag="ACFM8S_001480"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFC6314540.1"
                     /translation="MCGIVGAIRAHHNVVDFLTDGLKRLEYRGYDSSGIAVNTDGKIK
                     RVRRVGRVQLMEDAAREKGISGSIGIGHTRWATHGGVTEPNAHPHISGGMIAVVHNGI
                     IENFESERKRLEGLGYRFESQTDTEVIAHSINHEYAQNGGKLFEAVQEAVKRFHGAYA
                     IAVIAQDKPDELVVARMGCPLLVALGDDETFIASDVSAVIAFTRRVAYLEDGDIALLA
                     SDGIKRLTDKNGLPAERKVKVSELSLTSLELGPYSHFMQKEIHEQPRAIADTAEVFLD
                     GGFIPENFGKNAKSVFESIRSVKILACGTSYYAALTAKYWLESIAKIPADVEIASEYR
                     YRSVIADPDQLVITISQSGETLDTMEALKYAKSLGHRYSLSICNVMESALPRESSLVL
                     YTRAGAEIGVASTKAFTTQLVALFGLAVTLAKVRGLVSEEDEARYTEELRQLPGSVQH
                     ALNLEPQIAAWAQQFAKKTSALFLGRGIHYPIALEGALKLKEITYIHAEAYPAGELKH
                     GPLALVDENMPVVVIAPNDSLLDKVKANMQEVGARGGELFVFADLDSNFNATEGVHVI
                     RAPRHVGELSPVVHTIPVQLLSYHTALARGTDVDKPRNLAKSVTVE"
     gene            6426..6947
                     /locus_tag="ACFM8S_001481"
     CDS             6426..6947
                     /locus_tag="ACFM8S_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314541.1"
                     /translation="MKQMLLAVGVVAVLAGCGKDAGGYEGYWREKSDKKEGMIAVKKE
                     KGNYFLNKINVFTGKEESMLLSEKDGALSINTGIGEIPIKLSDDGKELYVERRQYVKT
                     DAAMKDKIIAHQKKCGQTAQAYLDARNALPSNQTYQQHQAAIEQLKRRFEAELDELEK
                     EIKCNGRSPALLL"
BASE COUNT         1511 a         1682 c         2142 g         1789 t
ORIGIN      
        1 gttgaagaag atgccacgcc ggcagaagtg ttgtttttcg gccatgtgcc gtgattcggg
       61 caatactcgg taacggctga ttttttgaaa gtgtttgaaa tcggcgcgtg gtgtttctat
      121 gcaaccggta gatgaatcat cgcggcaggt tgacggcagg tgcttggtgt tgattttgtc
      181 gtcggtcttg ccgttggcgg cggcgacgtc ggtggcggtg gtgcgcgtaa ccggctgtcc
      241 gcagaaccat ttgaccgaac cgtcttgacg cttggcccac agggagagtt ttttgtcttt
      301 gatttcgttg tttacgccgc ttgaagccat tatgtcagac ggtattgccc gggcagcttt
      361 attcgtacac tttcagcagc tcgacttcaa atatcaaagt ggcgtgcggg ggaatcacgc
      421 cgccggcacc gtgtgcgccg tagcccattt ccgaagggat ggtcagcttg cgtttgccgc
      481 cttccttcat tccgccgaag ccttcgtccc agcctttgat gacttgtccg acgccgagcg
      541 tgatggtcag cggctggcgg cggtcgaggc tggagtcgaa tttggttccg ttttccagcc
      601 aacctgtgta atgcacggta atctctttgc ctttaactgc ttcttttccg aagccttcct
      661 gcaagtcttc aataatcagg ccgcccatat ttgtcctttc gttgcttgtt ggtcaaaacg
      721 gcaagggtaa cataccgtcc ggcgaagtca aatgccgctc aaacgtcagc tgcatcggtg
      781 cagctgaaac ggctgtgttt gtttgactgt tttatttttt tcgtaaaggt tccatgcttt
      841 ttcatggaaa tagaaaacga cggtgttgat taggggttcg accagcgcaa ctgctcccga
      901 tacgcctata ctgcccgtca gtacataggt tacactgaag gcgacgctga aatgcagtgc
      961 ggcaaaagtc agggttttaa gcatcatcct ctcccggatt ggacattgac ggagagatga
     1021 taaagattat cataaggctg cgcggtttaa atttgctatt tgttgttagt gtagataaat
     1081 cgttttttaa ataaggatag gaattatgaa tcataaaaag atagtcgtat tggatgcgga
     1141 tactttgccc ggccgggttt ttcattttga ttttccgcac gagcttgcgg tttacggtac
     1201 gacaggtgcg gatgaaacgg cagaacgggt gcgcgacgca catattgtca ttaccaataa
     1261 ggtaatgatt tccgccgaca ttattgcggc taatccgcag ttggagctga ttgccgtcag
     1321 tgcgaccggt gtgaacaatg tcgatattgg ggcggcaaag gcggcgggcg ttgcggtatg
     1381 caatgtccgc gcatacggaa acgaatcggt tgcggaacac gcctttatgc tgatgattgc
     1441 cttgatgcgg aatttgcccg cctatcagcg tgatgttgcg gcaggattat gggagaagtc
     1501 gccatttttc tgtcattacg gcgcgccgat tcgggatttg aacggcaaaa cgctggcggt
     1561 tttcggacgc ggcaatatcg ggaggacgct ggccggatac gcgcaggcat tcggtatggg
     1621 ggtggtgttt gccgaacaca aacacgcgcc cgacgtgcgc gaaggctatg tttcctttga
     1681 agatgcggta cggactgccg acgtgttgtc gctgcactgt ccgctgaacg cccaaactga
     1741 aaatatgata ggcgaaaacg aattacagca gatgaagccc ggtgcggttt tgattaattg
     1801 cggacgcggc gggctggtgg atgaaaacgc gctgattgcc gcactcaaat acgggcagat
     1861 cggcggagct ggtgtcgatg ttttgacgga ggagccgcca aggggcggca atcccttgct
     1921 gaatgcacga ttacccaatc tgattgttac gccgcatacc gcgtgggcga gccgcgaggc
     1981 tttggacagg ctgttcgata tattgttggc gaacattcac gcctttgtga aaggcgaggc
     2041 gcaaaaccgc gtggtttgaa cctgtcggga ttgcggaaaa aaatgccgtc tgaacgccca
     2101 aagggttcag acggcattgt tttgatattt ctgcttaacc gactttgtcg cccggttgcg
     2161 cgcctgcgtc gacatccaag agcttcagtt tcccatctgc cgtggcggca ctcaaaatca
     2221 taccttcgga tacgccgaat tttgccattt tgcgcggtgc gaagttggcg acggcgatga
     2281 ccatgcggcc gttcaattcg gcaggatttg ggtaagacgc ggcgatgccg gagaagatga
     2341 tgcgtttttc aaaaccgaaa tcgaggtcga atttcaaaag tttggtgctg ccttcgacgg
     2401 cttcgcagtt caatactttg gcgacgcgca tgtcgatttt cataaagtcg tcgaagctgg
     2461 cctgttcggc gactttttcg tatttgccct cttcggcggc aggtgcggct gcggcggcga
     2521 tgctttgttt gttggcttcg attaaatcgt ccacttgttt ttgctccact cgttgcatta
     2581 aatgttcgta tttgttgatg gcgtgttcac ccaaggttac ccttgtgttc gcccaggtaa
     2641 tcgcgtccag attgaggaaa cgcgcggcgt tggcggcggt ttgcggcaac acgggggcga
     2701 ggtaggcggt cagtatggtg aaggcgttga tgagttcgct gcatacttcg tgcaggcgtt
     2761 cgtcttgacc ttcctgtttc gccagttccc aaggtttgtt ggcatcaacg tattcgttca
     2821 cggcatctgc caatgccatg atatcgcgca gggcgcgggc gtattcgcgg ttttcgtatt
     2881 gctcggcgat ggtgtcgctt tcggcggcaa gttttgccag caatgcgctg tcggcaacgt
     2941 ctttcagacg gccttcaaag cgtttggcga taaaacctga ggcgcgggcg gcgatgttga
     3001 cgtatttgcc gacaaggtcg ctgtttacgc ggctgataaa gtcttgcagg ttcaaatcga
     3061 tgtcttcgat tttgctgttg agtttggcgg cgatgtagta gcgcatccac tcggggttta
     3121 aaccttgttc caaataggat ttggcggtga taaacgtgcc gcgcgatttg gacatttttt
     3181 gtccatctac ggtcaaaaag ccgtgtgcgt acacgccggt cggggcgcgg tggccggaga
     3241 aatgcagcat ggcgggccag aacagggcgt ggaaatagag gatgtctttg ccgatgaaat
     3301 ggtacatctc ggtttggctg tcggctttga agtattcgtc gaaatcgacg ccgatacggt
     3361 cgcacaggtt tttaaacgac gccatgtagc cgacgggcgc gtccagccaa acgtagaagt
     3421 atttgccggg cgcgtcgggg atttcgaaac cgaaatacgg cgcgtctcgg gagatgtccc
     3481 agtcggatag ggtggtttct tcgccttcgc ccagccattc tttcattttg ttgagggctt
     3541 ccggttgcag atggggcttg ccgtcgtgcg ggttgttgcc ggaagtccat gctttgagga
     3601 agtcggcgca ttcgcccagt ttgaagaaga agtgttcgga ttcgcgcaat tcgggtttgg
     3661 caccggaaac ggcggaatac gggttgatca gttcggtcgg ggaataggtc gtgccgcaga
     3721 cttcgcagtt gtcgccgtat tggtcttggg cgtggcattt cgggcattcg cctttgacga
     3781 agcggtcggg caggaacatt tgtttttcgg ggtcgaaaag ctgctcgatg acgcggctct
     3841 caatcttgcc gttggctttc agcgcgcggt aaatgtcttg ggaaaactgt ttgttttcag
     3901 gggaatgggt gctgtaataa ttgtcgtaac cgatgaaaaa gccagtaaag tcggcgaggt
     3961 gctcttcgcg cactttggca atcatgtctt cgggggcgat accttgtttt tgcgcggcaa
     4021 gcatcacggg cgtgccgtgg gtgtcgtcgg cgcagcagta gtggcattcg ttgccgcgca
     4081 gtttttggaa gcgtacccaa acgtcggttt ggatgtgttc gaccatgtgg ccgaggtgga
     4141 tgctgccgtt ggcatagggc agggcggagg taacgaggat tttgcgtgtc atattgtgct
     4201 ttgcaaacaa tgggtaaagg cggattatac cgcaaatcaa acggggaaat gccgtctgaa
     4261 gcctgaaaaa tcgggcttca gacggcatgt tcgcaaacta tccggactta ttcgaccgta
     4321 acggatttcg ccaggttgcg cggtttgtcc acatccgtgc cgcgtgcgag ggcggtgtgg
     4381 taggaaagga gctgcacggg gatagtatgc acgacggggg ataattctcc gacatggcgc
     4441 ggcgcgcgga taacgtgcac accttcggtg gcattaaaat tactgtcgag gtcggcaaag
     4501 acgaaaagtt cgccgccgcg cgcgccgact tcctgcatat tggctttgac tttgtccaac
     4561 aggctgtcgt tgggtgcgat gacgacgacg ggcatgtttt cgtccaccaa agcaagcggc
     4621 ccgtgcttca gttcgccggc aggataggct tcggcgtgga tgtaggtgat ttccttcagc
     4681 ttcaacgcac cttcgagggc aatcgggtaa tggatgccgc gccctaaaaa cagcgcgctg
     4741 gttttcttgg caaactgttg cgcccatgcg gcaatttgag gttcgaggtt cagagcgtgc
     4801 tgcacgctgc cgggaagctg gcggagttct tcggtgtaac gcgcttcgtc ttcttcggaa
     4861 accaaaccgc gcactttcgc cagcgttacc gccaaaccga acagcgcgac cagctgcgtg
     4921 gtaaacgcct tggtcgaggc gacgccgatt tccgcaccgg cacgggtata aagcacgagg
     4981 ctgctttcgc gcggcagggc ggattccatc acgttgcaga tggagaggct gtagcggtgt
     5041 cccaaagatt tggcgtattt caaagcctcc atcgtgtcca gcgtttcgcc ggattgggaa
     5101 atggtaatga ccagttggtc gggatcggca atcacgctgc ggtatcggta ttcgctggca
     5161 atttcgacat cggccgggat tttggcgatg gattccaacc aatatttggc ggtcagcgcg
     5221 gcgtaatagg aagtgccgca ggcaaggatt ttgacgctgc ggatgctttc aaacacgctt
     5281 ttggcgtttt tgccgaagtt ttcagggatg aagccgccgt cgaggaaaac ttccgccgtg
     5341 tccgcaatcg cgcgcggctg ctcgtggatt tccttttgca taaagtggct gtacgggccc
     5401 agttccaaag aggtgagcga gagttcggat accttgactt tgcgttcggc aggcaggccg
     5461 tttttatcgg tcagcctttt aatgccgtct gaagccagca gtgcgatgtc gccgtcttcg
     5521 aggtacgcca cgcggcgcgt aaaggcgatg acggcggaca cgtccgaagc gataaaggtt
     5581 tcatcgtcgc ccaaagcgac caaaagcggg cagcccatac gcgccacaac taattcatca
     5641 ggcttgtctt gggcaataac cgcgatggcg tatgcgccgt ggaaacgttt gaccgcttct
     5701 tgtactgctt caaacaattt gccgccgttt tgcgcgtatt cgtgattgat gctgtgggcg
     5761 atgacttcgg tatccgtttg cgattcaaaa cggtatccca aaccttccaa acgtttgcgt
     5821 tcgctttcaa agttttcgat gatgccgttg tggacgaccg caatcatccc gccgctgatg
     5881 tgcgggtggg cgttcggctc ggtaacgccg ccgtgtgtcg cccagcgcgt atgtccgatg
     5941 ccgatgctgc cgctgatgcc tttttcacgt gccgcgtcct ccataagctg cacgcgtccg
     6001 acacggcgca cgcgtttgat tttgccgtcg gtgttgacgg caatgcctga tgagtcataa
     6061 ccccggtatt cgaggcgttt gaggccgtcg gtcagaaaat cgacgacgtt gtgatgggcg
     6121 cggatggcgc cgacgatacc gcacataact gttccttagt atcaggttga aaaaaaacag
     6181 gcgcggacgg ctcccgtgcc gcaccttccg cttcggatta taaaccgcct agtgtcggaa
     6241 aacagcaaaa tgccgtctga aggcttgggc ttgctcaaaa aaaggaggga tttccctgtt
     6301 tatccgggaa gggtgttcag acggcattgc ctgccgccgg tttatagttt ttgcaaatca
     6361 atattgacaa gctaaaaaaa aaaaacaata tactcgcctg gtcttaatgt taaaggagta
     6421 tggaaatgaa acaaatgctt ttagccgtcg gcgtggtggc ggtgttggcg ggctgcggca
     6481 aggatgccgg cggttacgag ggttattggc gcgaaaaatc ggacaaaaaa gagggtatga
     6541 ttgccgtcaa aaaagaaaaa ggcaattact tccttaataa aatcaacgtg tttacaggta
     6601 aggaagaatc tatgcttttg tctgaaaaag acggcgcgct ttcgataaac acggggatag
     6661 gggaaatccc gatcaaactt tccgacgacg ggaaagagct gtatgtcgaa cgcaggcagt
     6721 atgtcaaaac cgatgcggcg atgaaggaca aaatcatcgc ccatcagaaa aagtgcggac
     6781 aaacggcaca ggcatatctc gacgcgcgaa atgcgttgcc gtcaaaccag acgtatcagc
     6841 agcatcaggc ggcgatcgag caattaaaac ggcggtttga ggccgagtta gacgaattgg
     6901 aaaaagaaat caaatgcaac ggcagaagcc cggcattgtt gctttagcgt aggacaacag
     6961 ggaggatgcc gccgtccgaa tcggacgtgc ggtttctgta ccggtacggg cgggcaggaa
     7021 tgtccgcttt ttttgttcgg acgcgtttga atatccgtcc gattccgacc gtttgcaagg
     7081 ggtatttccg ttcgggcgga aattatagtg gattaacaaa aacc
//
LOCUS       DAXHSF010000105         7075 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.105, whole genome shotgun sequence.
ACCESSION   DAXHSF010000105 DAXHSF010000000
VERSION     DAXHSF010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7075
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.105"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(324..1217)
                     /locus_tag="ACFM8S_001482"
     CDS             complement(324..1217)
                     /locus_tag="ACFM8S_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HFC6314542.1"
                     /translation="MPHPCQTVHTLTARVPKCRLKLNAARRQRYNRPQLFFPNIIMTT
                     TEHDNDDAFLLRYSRHILLDEIGIEGQQKLSGAHILVVGCGGLGTAALPYLAASGIGT
                     LTIADSDTVELHNLQRQVAFDEGDVGKPKAEALAGRLKRINHTVNVRAVNEKLDGCRL
                     TGLVQTADIVLDCCDNYATRQAVNRACVQTKTPLVSGAAVRFEGQLAVYRPDLPDSPC
                     YACLFDGGSASDGICSLFGVFSPLVGIIGSTQAAEALKILLDAGEPSHGRLAVYRALE
                     GGWQYFDLPRNPECPICGAER"
     gene            1260..3962
                     /gene="ppc"
                     /locus_tag="ACFM8S_001483"
     CDS             1260..3962
                     /gene="ppc"
                     /locus_tag="ACFM8S_001483"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFC6314543.1"
                     /translation="MQLHILNNPKDAALAADAEFLKQSLFNLLHEEASPLVVETVKLL
                     STSDDSAALIEKVLPQLDEQQTHDLTLACGLFAQILNIAEDVHHERRRQIHEEAGRGG
                     AEGSLTETVRRLKAGKADGKSVQRQLDNTSVTAVLTAHPTEVQRQTVLSFNRRIRALL
                     PQRERCTNADALARLRREIDTILLGLWQTSETRRHKLSVNDEINNGVSIFPMSFFEAL
                     PKLYRKMEHDFQTAYPDVCVPNILKIGGWIGGDRDGNPFVSAETLRFAFRRHADAVFR
                     FYRSELDKLYRELPLSIRRVKVNGDVTALSDKSPDEEIARAEEPYRRAIAYIMARAMG
                     KARALGLGMGCKFGFLEPYASAQEFLDDLKKLQRSLIDNGSRLLAEGRLADLIRSVSV
                     FGFHMMPLDLRQHAGKHADVVAELFQHAGLEDYNRLNEEQKQAVLLRELSHQRPLYSP
                     FITYSEHTRHELAIFNEARKIKDEFGEDAVTQSIISNCEQPSDLLALALLLKESGLLA
                     VENGKPRSRINIVPLFETIEALENACPVMETMFRLDWYDALLESRGNIQEIMLGYSDS
                     NKDGGYVTSSWCLYQAELGLVELFKKYDVRMRLFHGRGGSVGRGGGPSYQAILAQPAG
                     SVAGQIRITEQGEVITAKYADPGNAQRNLETLVAATLEAGILPDKKDPDAKLMQALSD
                     VSFKYYRELITHPDFIDYFLQTSPIQEIATLNLGSRPASRKTLARIQDLRAIPWVFSW
                     MQNRLMLPAWYGFGSAVETLCEDKPETLAALREHAQSNPFFQAMLSNMEQVMAKTDIT
                     LAENYAGLSESPEKAAVIFGMIKEEYQRSRKALLDLLQTEELLRDNRSLARSLALRIP
                     YLNALNGLQVAMLKRLRKEPDNPHALLMVHLTINGVAQGLRNTG"
     gene            4085..5074
                     /locus_tag="ACFM8S_001484"
     CDS             4085..5074
                     /locus_tag="ACFM8S_001484"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFC6314544.1"
                     /translation="MKITVIGAGSWGTALALHFSQHGNRVSLWTRNADQVRQMQEARE
                     NKRGLPGFSFPETLEVCADLADALKDSGLVLIVTSVAGLRSSAELLKQYGAGHLPVLA
                     ACKGFEQDTGLLTFQVLKEVLPDNKKIGVLSGPSFAQELAKQLPCAVVLASENQEWIE
                     ELVPQLNTTVMRLYGSTDVIGVAVGGAVKNVMAIATGLSDGLEYGLNARAALVTRGLA
                     EITRLASAMGAQPKTMMGLAGIGDLILTCTGALSRNRRVGLGLAEGKELHQVLVEIGH
                     VSEGVSTIEEVFNTACKYQIDMPITQTLLQLIRKEMTPQQVVERLMERSARFE"
     gene            5129..5458
                     /locus_tag="ACFM8S_001485"
     CDS             5129..5458
                     /locus_tag="ACFM8S_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314545.1"
                     /translation="MKQNIEKLESSVYTLVQKFETLVSENRRLKETVAELKRAHERQK
                     LEHETAVDELSEALLVQVGKLKEDLQNKIDSLTEENTRYRSLLEQSREKISALAARLP
                     QRQETQQ"
     gene            5469..5774
                     /locus_tag="ACFM8S_001486"
     CDS             5469..5774
                     /locus_tag="ACFM8S_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFC6314546.1"
                     /translation="MNIEQVHIEVMHARLTVNTPAEEKDTLLQAVGMLNGKAEAIREG
                     GRVADSEKIVIMAALNVVHDLLKTSLNGGDLAIGDFARKIADMDNACQKALSRLAQE"
     gene            5780..5959
                     /gene="ssrS"
                     /locus_tag="ACFM8S_001487"
     ncRNA           5780..5959
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACFM8S_001487"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            6137..6568
                     /gene="rplM"
                     /locus_tag="ACFM8S_001488"
     CDS             6137..6568
                     /gene="rplM"
                     /locus_tag="ACFM8S_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFC6314547.1"
                     /translation="MKTFSAKPHEVKREWFVIDAQDKVLGRVAAEVASRLRGKHKPEY
                     TPHVDTGDYIIVINADKLRVTGAKFEDKKYFRHSGFPGGIYERTFREMQEQFPGRALE
                     QAVKGMLPKGPLGYAMIKKLKVYAGAEHAHAAQQPKVLELK"
     gene            6578..6970
                     /gene="rpsI"
                     /locus_tag="ACFM8S_001489"
     CDS             6578..6970
                     /gene="rpsI"
                     /locus_tag="ACFM8S_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFC6314548.1"
                     /translation="MNGKYYYGTGRRKSSVARVFLIKGTGQIIVNGRPVDEFFARETS
                     RMVVRQPLVLTENAESFDIKVNVVGGGETGQSGAIRHGITRALIDFDAALKPALSQAG
                     FVTRDAREVERKKPGLRKARRAKQFSKR"
BASE COUNT         1810 a         2058 c         1823 g         1384 t
ORIGIN      
        1 tttaatcagt cggacaaaat gccttttatc cgatggaacc gttttccgcc cgaacggaaa
       61 taagcggttt tcccctgtaa acagataaaa ccaaccgggt attcatacac agccaaaaaa
      121 aagccgtccg aacccaaagg ggacagacgg ccaaacacat tacggggaaa acgtcttact
      181 caatgagtct gcgaaacaga cattgctaaa acaactgcaa ttataaggta agtttatttt
      241 tttgcaaaga aatgtaaatt tttaaatttt taaaaccgtt ccgccgcatc cgtttggata
      301 ccgtctgaaa cggcggcagg gttttaccgt tccgcgccgc aaatcgggca ttcggggttg
      361 cgcggcaggt cgaaatattg ccagccccct tccaaggcac ggtaaaccgc cagcctgccg
      421 tgcgacggtt cgcccgcatc cagcaggatt ttcagagcct ccgccgcttg ggtactgccg
      481 atgatgccga ccagcggcga gaacacgccg aagagggaac agatgccgtc tgaagccgat
      541 ccgccgtcaa acaggcaggc gtaacacggc gagtcgggca agtcgggacg gtacacggca
      601 agttgccctt caaagcgcac cgctgctcct gaaaccagcg gtgttttcgt ttgcacgcag
      661 gcacggttga cggcttgccg cgtggcgtag ttgtcgcagc agtctaaaac gatgtcggcg
      721 gtttgaacca aaccggtcag gcggcagccg tcaagttttt cgttgacggc gcggacgttg
      781 acggtatggt tgatgcgttt cagacggcct gctaaggctt cggctttggg cttgccgaca
      841 tcgccctcgt caaatgcgac ttggcgttgc aggttgtgca gttcgaccgt gtcggaatcg
      901 gctatggtca gcgtgccgat gccagaagcg gcaaggtagg gcagggcggc ggtacccaaa
      961 ccgccgcagc cgacaaccaa aatatgtgcg ccggaaagtt tctgctgccc ttcgatgccg
     1021 atttcgtcca agaggatgtg gcggctgtac cgcagcagga atgcatcgtc gttgtcgtgt
     1081 tcggtcgtgg tcatgatgat gttcggaaaa aacagttgcg ggcgattgta acgctgccgt
     1141 cgggcggcgt tcaacttcag acggcatttc gggacacggg cggttaaagt gtgaacggtt
     1201 tggcacggat gcggcatttg aggtacattt acagtattta cggcaatgag agaaaaatca
     1261 tgcaactgca tattctgaac aatccgaagg acgcggcttt ggcggcggac gcggaatttc
     1321 tgaaacaatc cctgttcaac ctcctgcacg aagaagcctc gccgttggtt gtcgaaacgg
     1381 tcaaactctt gtctacttcc gacgacagcg cggcattgat tgaaaaagta ttgccgcaat
     1441 tggacgaaca acaaacccac gatttaacct tggcctgcgg cctgttcgcc cagattttga
     1501 acatcgccga agacgtgcac cacgaacgcc gccgccaaat ccacgaagaa gccggacgcg
     1561 gcggcgcgga aggcagcctg acggaaaccg tccgcaggct aaaagcgggg aaagccgacg
     1621 gcaaatcggt gcagcggcag ttggacaata cgtccgttac cgccgttttg accgcgcacc
     1681 cgaccgaagt gcaacgccaa accgtcttaa gcttcaaccg ccgcatccgc gcactgttgc
     1741 cgcaacgcga acgctgcacc aatgccgacg cgctggcacg gctgcgccgc gaaatcgaca
     1801 ctatcctgct gggcttgtgg cagaccagcg aaacgcgccg ccacaaactc agcgtcaacg
     1861 acgaaatcaa caacggcgtg tccatcttcc cgatgagctt tttcgaagcc ctgcccaagc
     1921 tctaccgcaa gatggaacac gactttcaga cggcctatcc cgatgtctgc gttccgaata
     1981 tcctcaaaat cggcggctgg atcggcggcg accgcgacgg caatccgttc gtttctgccg
     2041 aaaccttgcg ttttgccttc cgccgccacg ccgatgccgt gttccgtttt taccgcagcg
     2101 aactcgacaa actctaccgc gagttgccgc tctccatccg ccgcgtcaaa gtcaacggcg
     2161 atgtaacggc gttgtccgac aaatcgcccg acgaagaaat cgcccgcgcc gaagaaccct
     2221 accgccgcgc catcgcctac attatggcgc gcgctatggg caaagcgcgc gcgctcggtt
     2281 tgggcatggg ctgcaaattc ggctttctcg agccttatgc ttcggcacaa gaatttctcg
     2341 atgatttgaa aaaattgcaa cgttccctta tcgacaacgg cagccgcctg cttgccgaag
     2401 gccgtttggc agacctcatc cgttccgtgt ccgtgttcgg cttccacatg atgccgctcg
     2461 acctgcgcca acacgcaggc aaacacgccg atgtagttgc cgagcttttc caacacgcag
     2521 gcttggaaga ctacaaccgc ctgaacgaag aacaaaaaca agccgtcctg ttgcgagaat
     2581 tgagccatca acgtccgctg tacagcccgt tcatcacata cagcgaacac acccgccacg
     2641 aactggcaat tttcaacgaa gcgcgcaaaa tcaaagacga atttggcgaa gatgccgtaa
     2701 cacaaagcat tatttccaac tgcgaacaac ccagcgacct gctcgccttg gcattgctgc
     2761 tgaaagaaag cggcctgttg gcggtggaaa acggcaaacc gcgcagccgc atcaatatcg
     2821 tgccgctgtt tgaaaccatc gaagcactcg aaaacgcctg tccggtcatg gaaaccatgt
     2881 tccgcctcga ttggtacgat gccctgctcg aaagccgcgg caacatccaa gaaattatgc
     2941 tcggctactc cgactccaac aaagacggcg gctatgttac cagctcatgg tgtctgtatc
     3001 aggcggaatt gggcttggtc gaactcttca aaaaatacga tgtccgcatg cgcctgttcc
     3061 acggtcgcgg cggcagcgtc ggacgcggcg gcggtccgtc ttaccaagcc attctcgccc
     3121 aaccggcggg cagcgttgcc ggacaaatcc gcattaccga acaaggcgaa gtcattaccg
     3181 ccaaatacgc cgaccccggc aatgcccaac gcaacttgga aaccttggtt gccgcgactt
     3241 tggaagccgg catcctgccg gataaaaaag accctgatgc caaactgatg caggcattgt
     3301 cggacgtatc gttcaaatac taccgcgaac tgattaccca tcccgacttc atcgactact
     3361 tcctgcaaac cagcccgatt caggaaatcg ccaccctcaa cctcggcagc cgtcccgcca
     3421 gccgcaaaac cttggcacgg attcaggact tgcgcgcgat tccgtgggta ttttcctgga
     3481 tgcaaaaccg cctcatgctg ccggcttggt acggtttcgg cagcgcggtg gaaaccttgt
     3541 gcgaagacaa acccgaaacg ctcgccgccc tgcgcgaaca cgcccaaagc aacccgttct
     3601 tccaagccat gctttccaat atggagcaag tgatggcgaa aaccgacatc accctcgcgg
     3661 aaaactatgc cggcttgagc gaatcgccgg aaaaagccgc cgtcatcttc ggcatgatta
     3721 aggaagaata ccaacgcagc cgcaaagccc tgctcgacct gctgcaaacc gaagaacttt
     3781 tacgcgacaa ccgcagcctc gcccgttcgc ttgccttgag gattccctac ctgaacgcgc
     3841 tcaacggcct gcaggtcgcc atgctcaaac gcctgcgtaa agaacccgac aatccgcacg
     3901 cgctgctgat ggttcacctg accatcaacg gcgtggcgca aggtttgcgc aatacaggct
     3961 gattgtgccg catcggggca aaatgccgtc tgaacacctt tcagacggca tttccctgac
     4021 cgcacttgca gagaaacacc gattgtttta aagtgaacgg cagtgatatg ttgaaagacg
     4081 accaatgaaa attaccgtta tcggcgcagg ttcgtggggt acggcgctcg ccctgcactt
     4141 ttcccaacac ggcaaccgcg tatccctgtg gacgcgcaac gcagaccaag tccgccaaat
     4201 gcaggaagcg cgtgaaaaca aacgcggact gcccggcttt tcctttcccg aaaccttgga
     4261 agtgtgtgcg gatttggcag acgcgctcaa agacagcgga cttgtcctta tcgtaacctc
     4321 cgttgccgga ttgagaagca gcgcagagct gctcaaacag tacggcgcgg gacacctccc
     4381 cgtcctcgcc gcctgcaaag gattcgagca ggatacaggg ctgctgacct ttcaagtatt
     4441 gaaagaagta ttgcccgaca ataagaaaat cggcgtactc tccggcccga gttttgcaca
     4501 ggaactcgcc aaacaactgc cctgtgccgt cgtcctcgcc tccgaaaacc aagagtggat
     4561 tgaagaactc gtaccgcagc tcaacacgac tgtcatgagg ctttacggca gtaccgacgt
     4621 tatcggcgtg gcggttggcg gcgcggtaaa aaatgttatg gcgattgcca ccggattgtc
     4681 cgatggccta gagtacgggc ttaacgcccg tgccgcactg gttacgcgcg gattagctga
     4741 aattacccgc cttgcctccg caatgggcgc acagcccaaa accatgatgg ggctggcagg
     4801 catcggcgac ctcatcttga cctgcactgg cgcactctca cgcaaccgcc gcgtcggctt
     4861 gggtttggca gaaggcaagg aactgcatca ggtgctggtc gaaatcggac acgtttccga
     4921 aggggtcagc acgatagaag aagtcttcaa tactgcctgt aagtaccaaa tcgacatgcc
     4981 gattacccaa accctgctgc aactcatccg caaagaaatg accccgcaac aggtcgtcga
     5041 aaggctgatg gaacgcagcg cgcgttttga ataaacaaca gacagatgcc gtctgaagcc
     5101 ttcagacggc atacggacag gtaaggttat gaaacaaaat atcgaaaaac tcgaaagcag
     5161 cgtttatacg ctggtacaaa aattcgaaac cctcgtcagc gaaaaccgcc gcctcaaaga
     5221 aaccgtcgcc gaactcaaac gggcgcacga gcggcaaaaa ctcgaacacg aaaccgccgt
     5281 cgacgaactc agcgaagccc tgctcgtcca agtcggcaaa ctcaaagaag acctgcaaaa
     5341 caaaattgac agcctgacag aagaaaatac acgataccgc agcctgctcg aacagagcag
     5401 ggaaaaaatc agcgcactgg cagcgcgcct cccccaacgg caggaaacgc agcaataagg
     5461 attaaaggat gaacatcgaa caagtccaca tcgaagtcat gcacgcccgg ctgaccgtca
     5521 acacgccggc agaagaaaaa gacacactgt tgcaggcagt cggaatgctc aacggcaaag
     5581 ccgaagccat ccgcgaaggc ggacgcgtcg cggacagcga aaaaatcgtc attatggccg
     5641 cgctcaacgt cgtccacgac ctgttgaaaa cctccctgaa cggcggcgat ttggcaatcg
     5701 gcgattttgc gcgtaaaata gccgatatgg acaacgcctg ccaaaaagca ctatcccgct
     5761 tggcgcagga ataacccctt tttccctgcg gtgtccgcga aggcatatat tcctttgaac
     5821 caataagttc gcttaaggtt gccgggagca gtagtgggcg cgagcgtcct tttgcggacg
     5881 cacccgaaac tacccgaggc agccgccttg tcagcaaggt tcaagcggat tcggccgaaa
     5941 cggctctcgc ggggattccc attcaaaacc gcatcgcaac gatgcggttt ttcacattgg
     6001 ccgccgcccc gcccgaaaaa gcagtatgca gtctgccgaa atccggaaaa tccttccgta
     6061 acgcggtaat gcgattgaca aaatcgaatc ctgtctttaa aatttacaac tttctatatc
     6121 tatctggatt tccactatga aaaccttttc agcgaaaccc cacgaggtga agcgcgaatg
     6181 gttcgtcatc gatgcccaag acaaagtctt gggtcgcgtt gcggccgaag tcgccagccg
     6241 tctgcgtggc aaacacaaac ctgaatacac cccccacgtc gataccggcg attacatcat
     6301 tgttatcaat gcggacaaac tgcgtgtaac cggtgccaaa ttcgaagata aaaaatactt
     6361 ccgccattcc ggtttcccag gcggtatcta cgaacgcact ttccgcgaaa tgcaagagca
     6421 attcccgggc cgcgctttgg aacaagctgt aaaaggtatg ctgcccaaag gtcctttggg
     6481 ttacgccatg attaaaaaac tgaaagtgta tgcgggtgcg gaacacgccc atgctgcgca
     6541 acaacccaaa gttttggaac tgaaataagg acgcgacatg aacggtaaat actactacgg
     6601 cacaggccgc cgcaaaagtt cagtggctcg tgtattcctg attaaaggta caggtcaaat
     6661 catcgtaaac ggccgtcccg ttgacgaatt cttcgcacgc gaaaccagcc gaatggttgt
     6721 tcgccaaccc ttggttctga ctgaaaacgc cgaatctttc gacatcaaag tcaatgttgt
     6781 tggcggcggc gaaaccggcc agtccggcgc aatccgccac ggcattaccc gtgccctgat
     6841 cgacttcgat gccgcgttga aacccgcctt gtctcaagcc ggtttcgtta ctcgcgatgc
     6901 ccgcgaagtc gaacgtaaaa aaccgggtct gcgcaaagca cgccgtgcaa aacaattctc
     6961 caaacgttaa tgttggaaat tcaaaaaacc ctgcttatcg cagggttttt tatttgtagt
     7021 agacggtttc ccattggcaa tctaaagatt acagattggg caaaaatcaa aaaca
//
LOCUS       DAXHSF010000106         6962 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.106, whole genome shotgun sequence.
ACCESSION   DAXHSF010000106 DAXHSF010000000
VERSION     DAXHSF010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6962
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.106"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(73..1494)
                     /locus_tag="ACFM8S_001490"
     CDS             complement(73..1494)
                     /locus_tag="ACFM8S_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HFC6314549.1"
                     /translation="MRHLPLFSLMLFPASVYAADLDGANLNLLWSLPFALILLSIALG
                     PLFFSHTWHHHYGKITAFWTLLFLIPFSLVFGASAGIHTVAHALVEEYIPFILLLLAL
                     YTISGGILVWGDLNGTPKLNTALLAVGTALASIMGTTGAAMLMIRPLLKANQNRTRRV
                     HIVIFFIFLVANIGGGLTPLGDPPLFLGFLKGVDFMWTVKHMFAPVLISTAVLLTAFY
                     FIDNRFFKQESIAQDTPAQQEKPEKIAIFGKWNFLLLSGVVGAVLMSGLWKPEHPGFE
                     ILGSRYALQNLVRDVILIALTAVSMAITPKQVRAGNEFNFEPIAEVGKLFLGIFITIF
                     PVLSILKAGEAGALGGVVSLVHDTAGHPINVMYFWMSGILSAFLDNAPTYLVFFNMAG
                     GDAQALMTGTLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMPTFFGYMM
                     WSVAFLTPVFIVHTLIFFVFKLL"
     gene            complement(1852..2211)
                     /locus_tag="ACFM8S_001491"
     CDS             complement(1852..2211)
                     /locus_tag="ACFM8S_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HFC6314550.1"
                     /translation="MIRYLLIACGCISLLLGIIGIFLPLLPTTPFVLLSAACWAKASP
                     RFHRWLHRHRYFGPMVHNWEQNGAVPRKAKIFAISMMTASCLIMFWQFPQRWWVGAVS
                     SVFCSLVAIWMWRRPES"
     gene            complement(2208..2804)
                     /locus_tag="ACFM8S_001492"
     CDS             complement(2208..2804)
                     /locus_tag="ACFM8S_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HFC6314551.1"
                     /translation="MDTDCFSHQEHIAQATRQWLEKAVIGLNLCPFSKAPYIKNLVRI
                     TISKARHLDGFLEDLDKELQILGKSMPSEIETTLLVHPTLFPDFSRFNDFLDLADTAI
                     IENGLEGIVQIAPFHPQFRFQNSEPDDIGNYTNRSPYPTLHLIREDSIAKAAQAFPDA
                     SAIFERNIALLEKMGHEGWAKLGITSCPYPHNKKNISK"
     gene            complement(2822..3304)
                     /locus_tag="ACFM8S_001493"
     CDS             complement(2822..3304)
                     /locus_tag="ACFM8S_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFC6314552.1"
                     /translation="MPQLTLDKTDIKILQVLQENGRLTNVELSERVALSPSPCLRRLK
                     QLEDAGIVRQYAALLSPESVNLGLQAFIRVSIRKAKDAREDFAASVRKWPEVLSCFAL
                     TGETDYLLQAFFTDMNAFSHFVLDTLLSHHGVQDAQSSFVLKEIKHTTSLPLNHLLKE
                     "
     regulatory      3508..3605
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            3816..4916
                     /gene="gcvT"
                     /locus_tag="ACFM8S_001494"
     CDS             3816..4916
                     /gene="gcvT"
                     /locus_tag="ACFM8S_001494"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFC6314553.1"
                     /translation="MTALKTTPFHQAHQDAGAKPVDFAGWELPIHYGSQIAEHEAVRT
                     DAGMFDVSHMLVTDVAGANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLI
                     VYRTNEAETQYRIVSNGATREKDTAQFHKVGQEFGVAFNPRYDLGMLAVQGPKAIEKL
                     LTVKPEWADVIHGLKPFQGADLGNDWFVARTGYTGEDGVEVILPGTEAGVFFKALQAA
                     GVQPCGLGARDTLRMEAGMNLYGNDMDDDTSPLEAGMGWTVDLKDESRDFVGKAALLA
                     LKEKGVAVKQVGLLLDKGGILRAHMEVLTDKGKGETTSGVFSPSLKQSIAIARVPKDF
                     DGDTAKVLIRGKEVDVRVLKLPFVRNGQKQFD"
     gene            5078..5464
                     /gene="gcvH"
                     /locus_tag="ACFM8S_001495"
     CDS             5078..5464
                     /gene="gcvH"
                     /locus_tag="ACFM8S_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFC6314554.1"
                     /translation="MSNNIPAELKYVASHEWLRLEEDGTITVGITHHAQELLGDIVFV
                     ELPEVGANLAAEEQAGVVESVKAASDVYAPIAGEVVAVNEDLPSAPETANSDSYGAGW
                     FFKIKPVNPADYDGLLTAEQYAGEVD"
     gene            5611..6858
                     /gene="hemA"
                     /locus_tag="ACFM8S_001496"
     CDS             5611..6858
                     /gene="hemA"
                     /locus_tag="ACFM8S_001496"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFC6314555.1"
                     /translation="MQLTAVGLNHQTAPLSIREKLAFAAAALPKAVRNLARSNAATEA
                     VILSTCNRTELYCVGDSEEIIRWLADYHSLPIEEIRPYLYALDMQETVRHAFRVACGL
                     DSMVLGEPQILGQIKDAVRVAQEQESMGKKLNALFQKTFSVAKEVRTDTAVGENSVSM
                     ASASVKLAEQIFPDIGDLNVLFIGAGEMIELVATYFAAKSPRLMTVANRTLARAQELC
                     DKLGVNAEPCLLSDLPAILHDYDVVVSSTASQLPIVGKGMVERALKQRQSMPLFMLDL
                     AVPRDIEAEVGDLNDAYLYTVDDMVNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQ
                     GRQSVPLIKALRDEGEKARKQVLENAMKQLAKGATAEEVLERLSVQLTNKLLHSPTQT
                     LNKAGEEDKDLVHAVAQIYHLDK"
BASE COUNT         1798 a         1658 c         1938 g         1568 t
ORIGIN      
        1 gtcggaaggg ggatattgat ttgattatgc cggaatttaa aatgccgtct gaatgttcag
       61 acggcatagc gtttacagca gtttgaaaac gaaaaagata agggtatgta cgatgaagac
      121 gggtgtcagg aaggcgaccg accacatcat atagccgaag aaagtcggca tcggtacgcc
      181 gcgctgttcg gcaatggcct tgaccatgaa gttcggtgcg ttgccgatgt aggtcagtgc
      241 gcccatgaat accgaaccca tagaaaccgc cagcagcgaa tgaaacaggg tacccgtcat
      301 caaggcttgg gcatcgccgc ccgccatatt gaaaaaaacg agataagtgg gcgcgttatc
      361 caagaatgcc gacaatatgc cgctcatcca aaaatacatc acattaatcg gatgacctgc
      421 cgtatcgtga accagcgata ccaccccgcc cagcgcgcct gcctcgcctg ctttcagaat
      481 gctcaggacg ggaaagatgg tgatgaagat gccgaggaag agtttgccca cttcggcgat
      541 gggttcaaag ttgaattcgt tgcctgcgcg gacttgtttg ggcgtgattg ccatagatac
      601 ggcggtcaat gcaatcagga tgacatcgcg gacgaggttt tgcagggcgt aacggctgcc
      661 gaggatttca aatcccgggt gttcgggttt ccaaaggccg gacattagaa ccgcgccgac
      721 cacgcccgaa agcaggagga agttccattt gccgaagatg gcgatttttt cgggtttttc
      781 ctgttgtgcc ggcgtatctt gtgcaatgct ttcctgtttg aagaaacggt tgtcgatgaa
      841 atagaaggcg gtcaacagga cagcggtgct gatcaggacg ggggcgaaca tatgtttgac
      901 cgtccacatg aaatctacgc ctttgaggaa gccgaggaag agtggggggt cgcccaaagg
      961 ggtcagaccg ccgccgatgt ttgcaaccag gaaaatgaag aagatgacga tgtgcacgcg
     1021 gcgggtacgg ttttggttgg ctttcagcag cggacgaatc atcagcattg ctgcgccggt
     1081 cgttcccatg atagaggcaa gtgccgtacc gacggcaagc agggcggtgt tgagcttggg
     1141 tgtgccgttc aagtcgcccc aaaccaaaat gccgcctgaa atggtgtaca gggcaagcag
     1201 cagcaggatg aaggggatgt attcttcaac gagtgcgtgt gcgacggtat ggataccggc
     1261 ggacgcgcca aaaaccaaac tgaacgggat gaggaagagc aatgtccaaa aggcggtaat
     1321 tttgccgtaa tggtgatgcc aggtatgcga aaaaaacaag ggacccaatg cgatagacag
     1381 caaaatcagg gcaaagggca ggctccacag caggtttagg tttgcgccgt ccaaatctgc
     1441 ggcgtaaacc gatgcgggga aaagcattag tgaaaacagg ggtaggtggc gcatcgtgtt
     1501 tcctcgattc aagcactgcc ttgcgcggcg cgtgggagtg atacaggcac cgtgccgccc
     1561 ggacataggc ggactgtgct gcctgtttgt ttttgaaaaa agttttccga ttgattgtaa
     1621 agtaaataat caggataaac cagtttccca aaccggaagg cggcgggaag gcggattgct
     1681 gtgcttggga atatatgttc tttttgataa ataattttat ttaaaacaaa taaatatatg
     1741 tctttaataa ataatctatc gaaaacgaaa aatgaattta ttttaacata tatttgcaat
     1801 gaaacaggtt tgccccccgt ttgtttgccc ttatcccttt cagtacggca ttcaagattc
     1861 gggcctgcgc cacatccata tggcgacaag ggaacaaaaa accgatgaaa ccgccccgac
     1921 ccaccagcgt tggggaaact gccaaaacat tatcaggcag gatgcggtca tcatactgat
     1981 ggcgaatatt ttggctttgc gcggcactgc gccgttttgt tcccagttat gaaccatcgg
     2041 accgaaatag cggtgccggt gcagccagcg gtgaaagcgc ggggatgcct ttgcccagca
     2101 ggcggcggag agcagtacga acggcgtggt cggcaacagc ggcaaaaaaa tgccgatgat
     2161 acccaacagc agggaaatgc agccgcaggc aattaaaaga taacgtatca ttttgaaata
     2221 tttttcttat tgtgcggata agggcaggat gtgataccga gttttgccca gccttcatgt
     2281 cccatttttt ccagcagggc gatattgcgt tcgaatattg ccgaagcgtc gggaaaggct
     2341 tgtgcggctt tggcaatgct gtcttcgcgg atgaggtgca gcgtcggata gggagaacgg
     2401 ttggtgtagt tgccgatgtc gtcgggttcg ctattctgaa agcggaattg gggatggaac
     2461 ggtgcgattt ggacgatgcc ttccaagccg ttttcaataa tggcggtatc ggcaagatcg
     2521 agaaagtcat taaaccggct aaaatcgggg aataaggtcg ggtgaaccag tagcgtggtt
     2581 tcaatttcag acggcatcga tttgcctaaa atttgcaatt ccttgtctaa atcttctaaa
     2641 aagccgtcta aatgccgcgc cttgctgatg gtaatgcgga cgaggttttt gatatacggt
     2701 gctttggaaa aggggcataa attcaagccg ataacggctt tttccaacca ttgccgggtg
     2761 gcttgggcaa tgtgttcttg atgtgaaaag cagtcggtat ccataaatgg gtatcggaaa
     2821 atcattcttt caacaaatgg ttgagcggca gggatgtggt gtgtttgatt tcttttaaaa
     2881 caaagctcga ttgggcatcc tgtacgccgt ggtgggacag gagcgtatcc aaaacaaaat
     2941 gggaaaacgc gttcatatcg gtaaaaaacg cctgaagcag gtagtcggtt tcccctgtca
     3001 gggcgaagca gctcaagact tccggccatt ttcggaccga tgcggcaaag tcttcccgcg
     3061 cgtcttttgc tttgcggatg gaaacgcgga taaatgcctg aagccccagg ttgacggatt
     3121 ccggagacag cagcgcggca tattggcgga cgatgccggc atcttccaac tgcttcagac
     3181 ggcgcaggca cggagaaggc gaaagtgcga cacgttcgga cagttcgaca ttggtcagcc
     3241 tgccgttttc ctggagaacc tgtaagattt taatatcggt tttgtctaaa gtgagttggg
     3301 gcatatttgc gttccgtttt aaggaattcg gattgtctgt ccgtatgttt gcggcaatcc
     3361 gcacagatgg agaccatatt aacatataaa aagttatacc gtcatccggg acaaattttg
     3421 ttttcggaaa atcatgtgaa aacagaggcg gtcggtttgc atctctttta agacggcttg
     3481 cccaaaccgc cgattcaaga cataatcggg aaatgtgcag gagagtgtta cacccaacta
     3541 caatgtaacc accgaaggcg cagacaccct taaatcgctc aggtatcagg gactgcacat
     3601 tgaaacaaac aatctggaga gcggcgttag aataacgtcc accgaagggg agaaggccgt
     3661 ctgaaccacc attcagacaa ccgcgcaaag cagtgagcag actggtttgc catcatgcgg
     3721 atacggccga aaatctcagg ttcaaggaca gatagggtca tccgcgcata ggtgcgcggg
     3781 cggcatctga acaaaaaatc cggagaaact tgagaatgac tgctctgaaa accaccccat
     3841 ttcatcaagc ccatcaagat gcaggcgcga agccggtaga ttttgccggc tgggagctgc
     3901 ccatccatta tggttcacaa atcgccgaac acgaagccgt gcgcaccgac gccggtatgt
     3961 ttgacgtatc ccatatgctt gttaccgacg tagcaggcgc aaatgccaaa gcctttttcc
     4021 gcaaactgat tgccaacgat gtcgccaaac tcgcttttgt cggcaaagcc ctttattctg
     4081 ctttgctcaa cgacaacggc ggcgtgattg acgacttgat cgtttaccgc accaacgagg
     4141 ccgaaaccca ataccgcatc gtgtccaacg gtgcgacccg cgaaaaagac acggcgcaat
     4201 tccacaaagt cggacaagag ttcggcgttg ccttcaatcc gcgctacgac ctcggcatgc
     4261 tcgccgtaca aggccctaaa gccatcgaaa aactcctgac cgtcaaacct gaatgggcgg
     4321 atgtgattca cggcctcaaa ccgttccaag gtgcagactt gggcaacgac tggtttgtcg
     4381 cccgcaccgg ctataccggc gaagacggcg tcgaagtgat tctgccgggt actgaagcag
     4441 gcgtattctt caaagccctg caagcagcag gcgtacaacc ctgcggcctc ggcgcgcgcg
     4501 ataccctgcg catggaagcc ggtatgaacc tctacggcaa cgatatggac gacgacacca
     4561 gcccgctcga agcaggcatg ggttggacgg ttgatttgaa agacgaaagc cgcgattttg
     4621 tcggtaaagc cgccttgctg gcattgaaag aaaaaggcgt tgccgtcaaa caggtcggtc
     4681 tgttgctcga caaaggcggc atcctgcgcg cgcatatgga agtgttgacc gacaaaggca
     4741 aaggcgaaac caccagcggc gtattctctc caagtctgaa acaatccatc gccatcgccc
     4801 gcgtgccgaa agactttgac ggcgataccg ccaaagttct gattcgcggc aaagaagtgg
     4861 acgtacgcgt actgaagctg ccgtttgtgc gcaacggaca gaaacagttt gattgatgcg
     4921 gtttcagacg gcattttcat ttcatatgcc gtctgaaagc aggttttaat tgttgtccga
     4981 tacggacgtt tgtagaaagc attgaacaag gcatctgtgg atattgattc atgcagatgc
     5041 cgtctgaaaa taacccctat caatggagta tcaaaccatg agcaacaaca tcccggccga
     5101 actgaaatac gttgccagcc atgaatggct gcgccttgaa gaagacggta cgattaccgt
     5161 cggcattacc caccacgcgc aagagctgtt gggcgacatc gtgttcgtcg agctgcccga
     5221 agtcggcgcg aaccttgccg ctgaagagca agccggtgtg gttgagtctg taaaagccgc
     5281 gtccgacgtg tacgcaccga ttgcaggcga agtcgttgcc gtcaacgaag atttgccaag
     5341 tgctccggaa actgccaaca gcgattctta cggcgcaggc tggttcttca aaatcaaacc
     5401 tgtcaatcct gccgattacg acggtctgct gactgccgaa caatacgcgg gcgaagtgga
     5461 ctaaatctct atcctgataa tgggatgact gatttgagaa actgcacttt cagacggcaa
     5521 ggatgcgttt ggaagtgcag tttttgtctg tgtcttcaaa gttcagttag gctataataa
     5581 cggtctatat tcatctttac cgattttttc atgcaactta ccgctgtcgg actcaatcat
     5641 caaaccgcac ctttaagcat acgggaaaag ctggcgtttg ccgccgccgc cctgcctaaa
     5701 gccgtccgca atcttgcccg aagcaatgcg gcaacggagg cggtaatcct ttctacctgc
     5761 aaccgcaccg agctttactg cgtcggtgat tcggaagaaa tcatccgatg gcttgccgat
     5821 taccacagtt tgccgattga agaaatccgt ccgtatctgt acgcgctgga tatgcaggaa
     5881 accgtgcgcc atgccttccg cgttgcctgc ggcttggatt cgatggtgtt gggcgagccg
     5941 cagattttag gacagattaa ggatgcggtc agggttgctc aagagcagga aagtatgggt
     6001 aagaaactca atgccctgtt ccaaaaaacc ttttctgttg ctaaagaggt ccgtaccgat
     6061 actgccgtcg gcgaaaactc ggtttccatg gcttccgctt ccgtcaaatt ggcggaacag
     6121 atttttcccg acatcggcga tttgaatgtc ttgtttatcg gcgcaggcga aatgattgag
     6181 ctggttgcca cttattttgc cgccaaaagt ccccggctga tgacggttgc caaccggacg
     6241 ctggcgcgtg cacaggagtt gtgcgacaag ctcggtgtca acgccgaacc gtgcctgctg
     6301 tccgatctgc ctgccattct gcacgattac gacgtagtgg tttcttcaac ggcaagccag
     6361 ttgcccattg tcggcaaagg catggtggag cgtgcattga aacaaaggca gagtatgccg
     6421 ttgttcatgc ttgatttggc agtgccgcgt gacattgaag cggaagtcgg cgatttgaat
     6481 gatgcctatc tttatacggt ggacgatatg gtcaacatcg tccaaagcgg caaggaggca
     6541 aggcagaagg ccgccgccgc cgccgaaacg ctggtatccg agaaggttgc cgaatttgtc
     6601 aggcagcagc agggcaggca gagcgtcccc ttgattaaag ccttgcggga cgagggcgag
     6661 aaagcgcgca agcaggtgtt ggaaaatgcg atgaaacagc ttgccaaagg cgcaacggca
     6721 gaagaggttt tggaacggct gtccgtccaa ctgaccaaca agctgctgca ttcgccgacc
     6781 caaactttga ataaggcggg ggaagaagat aaagatttgg ttcatgccgt cgcgcagatt
     6841 tatcatttgg acaaataacg gtgcgccgga aaaggatgcc gtctgaaggg gtttcagacg
     6901 gcatattttt gtgctgtctg acaacatcgt gaaatcccac attatatcga tgtaatcaca
     6961 aa
//
LOCUS       DAXHSF010000107         6943 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.107, whole genome shotgun sequence.
ACCESSION   DAXHSF010000107 DAXHSF010000000
VERSION     DAXHSF010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6943
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.107"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            74..703
                     /locus_tag="ACFM8S_001497"
     CDS             74..703
                     /locus_tag="ACFM8S_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HFC6314556.1"
                     /translation="MIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIYLGQF
                     LGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPIFLGIKVLLLGDSDGEAIAKEGLRK
                     DNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVALLTFLVMIYLLVFSAQKL
                     AQVPSVGETLEKYSRWFVAVVYLGLGVYILIENNSFDMLWTVSGQEKIL"
     gene            849..2525
                     /locus_tag="ACFM8S_001498"
     CDS             849..2525
                     /locus_tag="ACFM8S_001498"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HFC6314557.1"
                     /translation="MLTYTPPDARTPAKTHEKPWLLMLMAFAWLWPGVFSHDLWNPAE
                     PAVYTAVEALADSRVPLVAHLFGHADFGIPPVYLWVAAAFKHLLSPWAADPYDAARFA
                     GVFFAVVGLTSCGFAGFNFLGRHHGRSVVLILIGCIGLIPTVHFLNPAAAAFAAAGLV
                     LHGYSLARRRVIAASFLLGTGWTLMSLAAAYPAAFALMLPLPVLMFFRPWQSRRLMLT
                     AVASLAFALPLMTVYPLLLAKTQPALFAQWLDYHVFGTFGGVRHVQTAFSLFYYLKNL
                     LWFALPALPLAVWTVCRTRLFSTDWGILGVVWMLAVLVLLAVNPQRFQDNLVWLLPPL
                     ALFGAAQLDSLRRGAAAFVNWFGIMAFGLFAVFMWTGFFAMNYGWPAKLAERAAYFSP
                     YYVPDIDPIPMAVAVLFTPLWLWAITRKNIRGRQAVTNWAAGVTLTWALLMTLFLPWL
                     DAAKSHAPVVRSMEASLSPELKRELSDGIECIDIGGGDLHTRIVWTQYGTLPHRVGDV
                     QCRYRIVRLPQNADAPQGWQTVWQGARPRNKDSKFALIRKIGENILKTTD"
     gene            2627..3232
                     /locus_tag="ACFM8S_001499"
     CDS             2627..3232
                     /locus_tag="ACFM8S_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HFC6314558.1"
                     /translation="MMTLYSGITCPFSHRCRFVLYEKGMDFEIKDVDIYNKPEDLAVM
                     NPYNQVPVLVERDLVLHESNIINEYIDERFPHPQLMPGDPVMRGRGRLVLYRMEKELF
                     NHVQVLENPAATNKEQAKAREAIGNGLTMLAPSFSKSKYILGEDFSMIDVALAPLLWR
                     LDHYDVKLGKSAAPLLKYAERIFQREAFIEALTPAEKAMRK"
     gene            3304..3696
                     /locus_tag="ACFM8S_001500"
     CDS             3304..3696
                     /locus_tag="ACFM8S_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFC6314559.1"
                     /translation="MPTSTKPYVLRALCEWCSDNSLTPHILVWVNEHTRVPMQYVRDN
                     EIMLNIGATATQNLQIDNDWISFSARFGGQAHDIWIPVGHVLSLFARETGEGMGFELE
                     EYRPDTPPENTSAETAPRPAKKGLKLVK"
     gene            complement(3725..3874)
                     /locus_tag="ACFM8S_001501"
     CDS             complement(3725..3874)
                     /locus_tag="ACFM8S_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314560.1"
                     /translation="MPSEARIAGSDSIFNVPISNRQGQHGFDTPKRVLPINGFRLRPI
                     GQNAV"
     gene            complement(3895..4341)
                     /locus_tag="ACFM8S_001502"
     CDS             complement(3895..4341)
                     /locus_tag="ACFM8S_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFC6314561.1"
                     /translation="MSLKIRLTEDMKTAMRAKDQVSLGTIRLINAAVKQFEVDERTEA
                     DDAKITAILTKMVKQRKDSAKIYTEAGRRDLADKENAEIEVLHRYLPQMLSAGEIRTE
                     VEAAVAETGAAGMADMGKVMGVLKTRLAGKADMGEVNKILKAVLTA"
     gene            complement(4473..4685)
                     /gene="rpsU"
                     /locus_tag="ACFM8S_001503"
     CDS             complement(4473..4685)
                     /gene="rpsU"
                     /locus_tag="ACFM8S_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFC6314562.1"
                     /translation="MPAIRVKENEPFEVAMRRFKRAVEKTGLLTELRAREAYEKPTTE
                     RKRKKAAAVKRLQKRLRSQQLPPKMY"
     gene            complement(4974..6821)
                     /gene="ltgA"
                     /locus_tag="ACFM8S_001504"
     CDS             complement(4974..6821)
                     /gene="ltgA"
                     /locus_tag="ACFM8S_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase LtgA"
                     /protein_id="HFC6314563.1"
                     /translation="MYLPSMKHSLPLLAALVLAACSSTHAPSSKTLADNIETADLSAS
                     VPTRPAEPEGKTLADYGGYPSALDAVKQKNDAVVAAYLENAGDSAMAENVRNEWLKSL
                     GARRQWTLFAQEYAKLKPEGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLLE
                     QAAASGLLDGNNAWRRVRGLLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIGK
                     EARKSPNAAALLSEMESGLSLEQRSFAWGVLGHYQSQNLNVPAALDYYGKVADRRQLT
                     DDQIEWYARAALRARRWDELASVISHMPEKLQKSPTWLYWLARSRAATGNTQEAEKLY
                     KQAAATGRNFYAVLAGEELGRKIDTRNNVPDAGKNSVLRMAEDGAVKRALVLFQNSRT
                     TGDAKMRRQAQAEWRFATRGFDEDKLLTAAQTAFDHGFYDMAVNSAERTDRKLNYTLR
                     YISPFKDTVIRHAQNVNVDPAWVYGLIRQESRFVIGAQSHVGAQGLMQVMPATAREIA
                     GKIGMDAAQLYTADGNIRMGTWYMADTKRRLQNNEVLATAGYNAGPGRARRWQADTPL
                     EGAVYAETIPFSETRDYVKKVMTNAAYYASLFGAPHIPLKQRMGIVPAR"
BASE COUNT         1379 a         1838 c         1904 g         1822 t
ORIGIN      
        1 tggggttgaa gttgacatct acaaagttta atgtttgaat acattaaaaa aatatatttg
       61 aatgagatgt tttatgattc aaaatgttgt tacttcaata atcctgtatt ctgggacagc
      121 cgtagactta cttattatcc taatgttatt ttttgccaaa agaaaaagca gaaaagacat
      181 cattaacatc tatttaggac aatttctagg ctctgttagc ctgatattgc taagtttgct
      241 ttttgcattt gtcttagatt atattcctag taaagagatt ttaggtttgc tcggcttgat
      301 tccaattttc ctaggcatca aagttttgct gttaggagat tctgatggag aggctattgc
      361 caaagagggt ttgcgcaaag ataataaaaa cctgattttt ctagtcgcta tgattacttt
      421 tgcaagttgt ggtgctgaca atattggtgt ctttgtccca tattttacta ccttaaattt
      481 agcgaatttg atagtggctt tacttacctt tctagtcatg atttatctct tggttttttc
      541 tgcccaaaaa ttagcacaag tcccttctgt tggagaaact ttggaaaaat atagcagatg
      601 gtttgttgct gttgtttatt taggattggg ggtatatatc ctgattgaaa acaacagttt
      661 tgatatgcta tggactgtgt cgggccagga aaaaatatta tgaaaaagat agtatctgca
      721 aagactgcca tgtgcagtct tttttgttgc cggtcttttt gtgtctgatg ccgtctgaag
      781 cagtctctgc acgacctttg tgcgaatatt tgctacactt ggcaatccgt ccatttcttc
      841 tcacgcttat gctgacctat accccgcccg atgcccgaac acctgccaaa acacatgaaa
      901 aaccgtggct gctgatgctg atggcgtttg cctggttgtg gccgggtgta ttttcacatg
      961 atttatggaa tcctgccgaa cctgccgttt ataccgccgt cgaagcattg gcagacagcc
     1021 gcgttccttt ggttgcccat ctgttcgggc atgccgattt cggcatacca cccgtgtatc
     1081 tttgggttgc cgccgcgttc aaacatttgc tgtcgccgtg ggcagccgac ccgtatgatg
     1141 ccgcacgctt tgcaggcgtg tttttcgccg ttgtcgggct gacttcctgc ggctttgctg
     1201 gcttcaactt tttgggcaga caccacgggc gcagcgtcgt cctgattctc atcggctgta
     1261 tcgggctgat tccgaccgta cactttctca accccgctgc cgccgccttt gccgccgccg
     1321 gactggtgct gcacggttat tctttggctc gccggcgcgt gattgccgcc tcttttctgc
     1381 tcggtacggg ctggacgctg atgtcgttgg cagcagctta tccggcagca tttgccctga
     1441 tgctgccctt gcccgtactg atgtttttcc gtccgtggca aagcaggcgt ttgatgttga
     1501 cggcagtcgc ctcacttgcc tttgccctgc cgcttatgac cgtttacccg ctgctcttgg
     1561 caaaaacgca gcccgcgctg ttcgcgcaat ggctcgacta tcacgttttc ggtacgttcg
     1621 gcggcgtgcg gcacgttcag acggcattca gtttgtttta ctatctgaaa aacctgcttt
     1681 ggtttgcatt gcccgcgctg ccgctggcgg tttggacggt ttgccgcacg cgcctgtttt
     1741 cgaccgactg ggggattttg ggcgtcgtct ggatgcttgc cgttttggtg ctgcttgccg
     1801 tcaatccgca gcgttttcag gataacctcg tctggctgct tccgccgctt gccctgttcg
     1861 gcgcggcgca actggacagc ctgaggcgcg gcgcggcggc gtttgtcaac tggttcggca
     1921 ttatggcgtt cggactgttt gccgtgttca tgtggacggg ctttttcgcc atgaattacg
     1981 gctggcccgc caagcttgcc gaacgcgccg cctatttcag cccgtattat gttcctgata
     2041 tcgatcccat tccgatggcg gttgccgtac tgttcacacc cttgtggctg tgggcgatta
     2101 cccggaaaaa catacgcggc aggcaggcgg ttaccaactg ggcggcaggc gttaccctga
     2161 cctgggcttt gctgatgacg ctgttcctgc cgtggctgga cgcggcgaaa agccacgcgc
     2221 cggtcgtccg gagtatggag gcatcgcttt ccccggaatt aaaacgggag ctttcagacg
     2281 gcatcgagtg tatcgacata ggcggcggcg acctgcacac gcggattgtt tggacgcagt
     2341 acggcacatt gccgcaccgc gtcggcgatg tacaatgccg ctaccgcatc gtccgcttgc
     2401 cccaaaatgc ggatgcgcct caaggctggc agacggtctg gcagggtgcg cgcccgcgca
     2461 acaaagacag taagttcgca ctgatacgga aaatcgggga aaatatatta aaaacaacag
     2521 attgagccga atttctggat taagtgccgg aaatcgcgta taattgcgcg attaaacctt
     2581 tataaatcag ccgttttgta ggcatcacac aggagcgaca attattatga tgaccctcta
     2641 ttccggcatt acctgcccct tcagccaccg ctgccgcttc gttttgtacg aaaaaggcat
     2701 ggattttgaa atcaaagacg tcgatattta caacaagccc gaagacctcg ccgtcatgaa
     2761 cccgtataac caagttcccg tgctggtcga gcgcgatttg gtgctgcacg agtccaacat
     2821 catcaacgaa tacatcgacg aacgcttccc ccatccgcag cttatgcccg gtgatcccgt
     2881 tatgcgcggc aggggccggc tggtgctgta ccgtatggaa aaagaattgt tcaaccacgt
     2941 ccaagttttg gaaaaccccg ccgccaccaa caaggaacag gcaaaagcgc gcgaagccat
     3001 cggcaacggt ctgaccatgc ttgccccttc gttcagcaaa agcaaataca tcctcggcga
     3061 agatttttct atgattgatg tcgcccttgc tccgctgctg tggcggctcg accactacga
     3121 tgtcaaactg ggcaaaagtg ccgcgccgct gctcaaatac gccgagcgca tcttccagcg
     3181 cgaagccttt atcgaagcac tgacacccgc cgaaaaagcc atgcgcaaat aagtccgaaa
     3241 tgcttgcaaa acccaccgtt ttgcaggcat tttcctattt tggcgtacaa cacggaaccc
     3301 atcatgccca cctcaaccaa accctacgtc ctccgcgctc tctgcgaatg gtgcagcgac
     3361 aacagcctca caccgcacat ccttgtttgg gtcaacgaac acacgcgcgt ccccatgcag
     3421 tatgtccgcg acaatgaaat tatgctcaac atcggtgcga ccgccacgca aaaccttcaa
     3481 atcgacaacg attggatcag cttttccgcc cgtttcggcg gacaggcgca cgacatatgg
     3541 atacctgtcg gacacgtcct cagccttttc gcacgggaga ccggcgaagg tatggggttt
     3601 gaattggagg agtaccgccc cgacacgccg cctgaaaaca cctctgccga aaccgcgccc
     3661 cggcccgcca aaaaaggctt gaaattggtc aaataaatct atgccgtctg aacggaatcg
     3721 tgtttcagac ggcattttgt ccgatggggc gcaaacggaa tccgtttatc ggcaaaaccc
     3781 gtttcggtgt atcgaaaccg tgttgcccct gccgattcga tattgggaca ttgaaaatgc
     3841 tgtctgaacc tgcgatacgg gcttcagacg gcattttgtc cgatattcgg gcaatcaggc
     3901 ggtcagcacg gctttcagga ttttgttgac ttcgcccata tcggctttgc cggcaagtcg
     3961 ggttttcaat acgcccatca ctttacccat atccgccata cctgccgcgc cggtttcggc
     4021 aacggcggct tcgacctcgg tacggatttc gccggcagag agcatttggg gaaggtagcg
     4081 gtgcagtacc tcgatttcgg cgttttcttt gtctgccaaa tcccggcggc cggcttcagt
     4141 gtagattttc gcgctgtctt tgcgctgttt gaccattttg gtcaggatgg cggtgatttt
     4201 ggcatcgtcg gcttcggtgc gttcgtccac ttcaaactgt ttgacggcgg cgttgatgag
     4261 gcggatggtg ccgagggaaa cttggtcttt ggcgcgcatc gcggttttca tgtcttcggt
     4321 aaggcggatt ttcaggctca taatgtcctc gctgggatgt cggatggaaa cggcgggttt
     4381 cgatgccgtc tgaaaagcaa aacacaccgc agagcaggtc ttgcggtgtg tcctcatatc
     4441 acagggctga cctgtaatct gtacttgaac gtttagtaca ttttgggtgg cagttgttgg
     4501 ctgcgcaggc gtttttgcag gcgttttacg gctgccgctt ttttgcgttt gcgttcggta
     4561 gtcggttttt cgtaggcttc gcgggcgcgc agctcggtca gcaggccggt tttttctacg
     4621 gcgcgtttga aacggcgcat agcgacttca aatggttcat tctcttttac gcggattgca
     4681 ggcattttat ttcctttaat aaattcggtt gtttcatctg cccatcatat cggtggaaag
     4741 ggtagggcgg acggtgttga aggtttaccg tatcgctgtg ccttgcggcg gcgggatacg
     4801 cgccgtgacg gaaacatcac ctcctaacgg gtcggcttgt gccgtgtcaa gctgcttgaa
     4861 aaagcaggaa aaacaatttt cggattgtct tatatttatg gttcgccgtc aatgccgttc
     4921 gggataaaaa tgccgtctga aagaccgggc gggtttcaga cggcatcggt acgtcagcgt
     4981 gcaggaacaa tgcccatacg ctgtttgagc gggatgtgcg gcgcgccgaa gagggaggcg
     5041 tagtaggcgg cattggtcat cacttttttg acatagtcgc gcgtttcgga aaacgggatg
     5101 gtttcggcat ataccgcgcc ttcgaggggc gtgtccgcct gccatcggcg cgccctgccg
     5161 gggccggcgt tatagcctgc ggtggcgagg acttcgttgt tttgcaggcg gcgtttggtg
     5221 tccgccatat accacgtccc catacggata ttgccgtcgg cggtgtaaag ttgtgcggca
     5281 tccataccga ttttgccggc gatttcgcgc gcggtggcag gcataacctg catcagcccc
     5341 tgcgcgccta cgtgggattg cgcgcctata acgaagcggc tttcctgacg aatcagaccg
     5401 taaacccaag ccggatcgac attaacattt tgcgcgtggc ggattaccgt gtctttaaac
     5461 ggcgaaatat agcgcaaggt gtagttgagt ttgcggtcgg tgcgttccgc gctgttgacc
     5521 gccatatcgt aaaaaccgtg gtcgaacgcg gtttgcgcgg cggtcagcag cttgtcttcg
     5581 tcaaagccgc gtgtggcaaa acgccattcc gcctgagcct gacggcgcat tttcgcatcg
     5641 ccggtggttc ggctgttttg gaatagcacc agcgcgcgtt tgactgcgcc gtcttccgcc
     5701 atacggagga cgctgttttt gccggcatcg ggcacattgt tgcgcgtatc gattttgcga
     5761 cccaactctt cccctgccag caccgcataa aaattcctgc ccgttgctgc cgcctgtttg
     5821 taaagttttt ccgcctcttg cgtgttgccc gttgcggcgc ggctgcgtgc cagccagtag
     5881 agccaggtcg ggcttttttg cagtttttcg ggcatatgcg agattacgga agccagctcg
     5941 tcccaacgtc gggcgcgcaa ggcggcgcgg gcgtaccact cgatttggtc gtcggtcagt
     6001 tggcggcggt cggcaacctt gccgtaatag tccaaggcgg caggcacatt gaggttttgc
     6061 gactgataat gccccaatac gccccacgcg aaactgcgtt gttcgaggct taaaccgctt
     6121 tccatttcgg acagcagggc ggcggcattc ggcgatttgc gtgcttcttt gccgatgacg
     6181 ttcaacaggg catattcgcg cgaaccttgt gtaccgccgt caaacgggct gcccaatgcg
     6241 gcggcaaggt tgcgtgcgtc tgtggtttgg cggccggcca gcagtccgcg cacgcgcctc
     6301 caggcattgt tgccgtccaa caagccggat gcggctgcct gttccaacag tttggtgcag
     6361 cccgaaggca gtttgcccgt atttttgacc agttcggcgg cacgcgtata gtcgttgcgg
     6421 ctcgaatcgg cgtagcattc gacttcttgg gcgcgccctt caggtttgag tttggcatat
     6481 tcctgtgcaa acagcgtcca ctgtctgcgt gcgcccaaag acttcagcca ctcgttgcgg
     6541 acattttccg ccatcgcact gtcgccggcg ttttccaaat aggcggcgac gacggcatcg
     6601 tttttctgtt tcactgcatc cagtgcggac gggtagccgc cgtaatctgc cagcgttttt
     6661 ccttcgggtt cggcagggcg ggtgggaacg cttgccgaaa gatcggcagt ttctatattg
     6721 tctgccaggg tcttgctgct gggcgcgtgt gtcgaagaac acgcggcaag caccagggcc
     6781 gccagcagcg gcagggaatg cttcatagag ggtaggtaca tcggatttcc ttaagaatcg
     6841 gaaccctgaa cggtcagggt tggaaaagac aaaatgccgt ctgaacaggc gtttgcccga
     6901 attatatgcc gaaactgcac cgcctttgga atgtttccga cat
//
LOCUS       DAXHSF010000108         6838 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.108, whole genome shotgun sequence.
ACCESSION   DAXHSF010000108 DAXHSF010000000
VERSION     DAXHSF010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6838
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.108"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(76..1422)
                     /gene="mnmE"
                     /locus_tag="ACFM8S_001505"
     CDS             complement(76..1422)
                     /gene="mnmE"
                     /locus_tag="ACFM8S_001505"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFC6314564.1"
                     /translation="MSDNVPTIAAVATAPGRGGVGVIRISGKNLLPMAQALCGKTPKP
                     RTATYADFTDADGQAIDSGLLLFFAAPASFTGEDVIELQGHGGPVVMDMLLNRCLELG
                     ARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASSRSAARLALRSLKGDFSRRIHGLV
                     EDLITLRMLVEATLDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLN
                     VVLVGAPNVGKSSLLNALAGDEVAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRE
                     TDDVVERIGIERSRKAVSEADVALVLVDPREGLNEKTRAILDALPPELKRIEIHSKSD
                     LHAHAAGRFGTGAETVIALSAKTGDGLDALKRTLLREAGWQGESEGLFLARTRHVNAL
                     KAAQEELSLAALCGNHQIELFAEHLRLAQVACGEITGEFTADDLLGVIFSRFCIGK"
     gene            1553..1708
                     /locus_tag="ACFM8S_001506"
     CDS             1553..1708
                     /locus_tag="ACFM8S_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314565.1"
                     /translation="MEKRNTYAFLLGIGSLLGLFHPAKTAIRPNPADDLKNIGGDFQR
                     AIEKARK"
     gene            1705..2133
                     /locus_tag="ACFM8S_001507"
     CDS             1705..2133
                     /locus_tag="ACFM8S_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HFC6314566.1"
                     /translation="MTENAQDKARQAVETVVKSPELVEQILSDEYVQIMIARRFHSGP
                     LPPSDLAQYNDIISNGADRIMAMAEKEQAVRHETIRQDQTFNRRGQLYGFISVILILL
                     FAVFLVWSGYPATAASLAGGTVVALAGAFVIGRSRDQGKN"
     gene            2267..6619
                     /gene="app"
                     /locus_tag="ACFM8S_001508"
     CDS             2267..6619
                     /gene="app"
                     /locus_tag="ACFM8S_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_168458961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesion and penetration autotransporter App"
                     /protein_id="HFC6314567.1"
                     /translation="MKTTDKRTTETHRKAPKTGRIRFSPAYLAICLSFGILPQAWAGH
                     TYFGINYQYYRDFAENKGKFAVGAKDIEVYNKKGELVGKSMTKAPMIDFSVVSRNGVA
                     ALVGDQYIVSVAHNGGYNNVDFGAEGSNPDQHRFSYQIVKRNNYKAGTNGHPYGGDYH
                     MPRLHKFVTDAEPVEMTSDMRGNTYSDKEKYPERVRIGSGQQWQRTDEQQAKGDAYSS
                     WLAGAYNWRIAGNTHSQSGAGNGTVNLSGDITKSNNYGPLPTAGSFGDSGSPMFIYDA
                     IKQKWLINGVLQTGNPYLGAGNGFQLIRKDWFYGNVFAEDLPITFLEPRSNGHYSFSS
                     NNNGTGTVTQTNEKVSMPRFKVRTVQLFNEALKAEDKEPVYAAGGVNAYKPRLNNGKN
                     ISFIDYAKGEVTFTNNINQGAGGLYFEGDFTVSSENNATWQGAGVHISEDSTVTWKVN
                     GVANDRLSKIGKGTLHVQAKGENQGSISVGDGKVILDQQADENNKKQAFSEIGLVSGR
                     GTVQLNADNQFNPDKLYFGFRGGRLDLNGHSLSFHRIQNTDEGAMIVNHNQDKESTVT
                     ITGNKDITTTGNNNSLDSKKEIAYNGWFGEKDSTKTNGRLNLVYQPAAEDRTLLLSGG
                     TNLNGNITQTNGKLFFSGRPTPHAYNHLNDHWSQKEGIPRGEIVWDNDWIDRTFKAEN
                     FLIKGGQAVVSRNVAKVEGDWHLSNHAQAVFGVAPHQSHTICTRSDWTGLTNCVEKTI
                     TDEKVIASLTNTDISGNVSLANHAHLNLTGLATLNGNLTAGGDTHYEVTHNATQNGNL
                     SLVGNAQATFNQATLNGNTSASGNASFNLSDHAAQNGDLTLSGNAKANVSHSAFNGNV
                     SLADKAVFHFENSRLTGQISGGKDTALHLKDSEWTLPSGTELGNLNLDNATITLNSAY
                     RHDAAGAQTGSATDAPRRRSRRSLLSVTPPTSAESRFNTLTVNGKLNGQGTFRFMSEL
                     FGYRSDKLKLAESSEGTYTLAVNNTGNEPVSLDQLTVVEGKDNKPLSENLNFTLQNEH
                     VDAGAWRYQLIRKDGEFRLHNPVKEQELSDKLGKAEAKKQAGKDNAQSLDALIAAGRD
                     AVEKTESVAEPARQGGGENVGIMQAEEEKKRVQADKDSALAKQREAETRPATTAFPRA
                     RRARRDLPQPQPQPQPQRDLISRYTNSGLSEFSATLNSVFAVQDELDRVFAEDRRNAV
                     WTSGIRDTKHYRSQDFRAYRQQTDLRQIGMQKNLGSGRVGILFSHNRTENTFDDGIGN
                     SARLAHGAVFGQYGIGRFDIGISTGAGFSSGSLSDDIGSKIRRRVLHYGIQARYRAGF
                     GGFGIEPHIGATRYFVQKADYRYENVNIATPGLAFNRYRAGIKADYSFKPAQHISITP
                     YLSLSYTDAASGKVRTRVNTAVLAQDFGKTRSAEWGVNAEIKGFTLSLHAAAAKGPQL
                     EAQHSAGIKLGYRW"
BASE COUNT         1840 a         1930 c         1681 g         1387 t
ORIGIN      
        1 caaacggatc ggattcccgt ttttatggga taacggaatg ttcagccgga caccgccacg
       61 atgctttcga tccgtttatt ttccgatgca gaatctcgaa aaaatcacgc cgagcaggtc
      121 gtccgccgta aactcgcccg tgatttcgcc gcatgcgacc tgcgccagcc gcaagtgttc
      181 ggcaaacagc tcgatttgat ggttgccgca caatgccgcc agcgacaatt cttcctgcgc
      241 tgctttgagt gcgttgacgt gccgcgtccg cgccaaaaac agcccttcgc tttcgccctg
      301 ccaaccggcc tcgcgcaaca acgtccgttt cagcgcgtcc aagccgtcgc cggttttcgc
      361 cgacagcgcg atgacggttt ctgcgcccgt accgaacctg cctgccgcgt gtgcgtgcaa
      421 atcggatttg ctgtggattt cgatgcgttt caactccggc ggcaacgcgt ccaaaatcgc
      481 ccgtgtcttt tcattcaaac cctcgcgcgg atcgaccaac accagcgcga catcggcttc
      541 ggatacggct ttgcggctgc gttcgatgcc gatgcgttcg accacgtcgt ccgtctcgcg
      601 caaacctgcc gtatcgacaa tatgcactgg aacgccgtca atcaggatac gttccctgac
      661 cgcgtcgcgc gtcgttccgg caatatcggt aacaatcgcc acttcgtcgc ccgccagcgc
      721 gttcaacagg ctggatttgc cgacattcgg cgcgccgacc aatacgacat tcagaccttc
      781 gcgcaaaatc gcgccctgct gcgcgttggc aagcacatca tccacggcgc ggcgcaagcc
      841 gtccaatttg ccgcgcgcat ccgctgcttc gagaaaatca atgtcttcct cgggaaaatc
      901 cagcgtcgct tcgaccagca tccgcaaggt aatcaaatct tcgaccagac cgtgtatccg
      961 ccgcgaaaaa tcgcccttga gcgagcgcaa cgccagacgc gccgccgaac ggctggatgc
     1021 gtcaatcaaa tccgccacgc cctccgcctg tgccaagtcc agtttgtcgt ttaaaaacgc
     1081 acgcttcgta aactcgcccg gctcggcaag gcgcgcgccc agttccaaac agcggttcag
     1141 cagcatatcc atcaccaccg gcccgccgtg tccctgaagc tcgatgacat cttcgcccgt
     1201 aaaacttgcc ggtgcggcaa aaaacagcaa aagcccgcta tcgattgcct gtccgtccgc
     1261 gtccgtaaaa tcagcatagg ttgcggtacg cggtttaggc gttttcccgc acaaagcttg
     1321 cgccatcggc agcaggtttt tccccgatat gcgtatcacg cccacgccgc cgcgtccggg
     1381 cgcggtagcg actgccgcaa tcgttggaac gttatccgac ataaaacccc cgaaaattca
     1441 aaacagccgc gattatagca aatgccgtct gaagtccgac ggtttggctt tcagacggca
     1501 taaaaccgca aaaatgcttg ataaatccgt ccgcctgacc taatataacc atatggaaaa
     1561 acgaaacaca tacgccttcc tgctcggtat aggttcgctg ttgggcctgt tccatcccgc
     1621 aaaaaccgcc atccgcccca atcccgccga cgatctcaaa aacatcggcg gcgattttca
     1681 acgcgccata gagaaagcgc gcaaatgacc gaaaacgcac aggacaaggc gcggcaggct
     1741 gtcgaaaccg tcgtcaaatc cccggagctt gtcgagcaaa tcctgtccga cgagtacgtg
     1801 caaataatga tagctcggcg tttccattcg ggaccgttgc cgccgtccga cttggcgcaa
     1861 tacaacgaca ttatcagcaa cggggcagac cgcattatgg caatggcgga aaaagaacaa
     1921 gccgtccggc acgaaaccat acggcaagac caaaccttca acaggcgcgg gcaactgtac
     1981 ggcttcatca gcgtcatcct gatactgctt tttgccgtct tcctcgtatg gagcggctac
     2041 cccgcaaccg ccgcctccct tgccggcggc acagtggttg ccttggcggg tgctttcgtg
     2101 attggaagaa gccgagacca aggcaaaaat taatgacaaa tcctagggcg tgcttcatat
     2161 ccgcccgaac gccgaaccgc acatataggc acatcccgcg cgccgcccgg aagcggaagc
     2221 cgcgccctcc caaacaaacc cgaatcccgt cagataagga aaaataatga aaacaaccga
     2281 caaacggaca accgaaacac accgcaaagc cccgaaaacc ggccgcatcc gcttctcgcc
     2341 tgcttactta gccatatgcc tgtcgttcgg cattcttccc caagcctggg cgggacacac
     2401 ttatttcggc atcaactacc aatactatcg cgactttgcc gaaaataaag gcaagtttgc
     2461 agtcggggcg aaagatattg aggtttacaa caaaaaaggg gagttggtcg gcaaatcaat
     2521 gacaaaagcc ccgatgattg atttttctgt ggtgtcgcgt aacggcgtgg cggcattggt
     2581 gggcgatcaa tatattgtga gcgtggcaca taacggcggc tataacaacg ttgattttgg
     2641 tgcggaggga agcaatcccg atcagcaccg cttttcttac caaattgtga aaagaaataa
     2701 ttataaagca gggactaacg gtcatcctta tggcggcgat tatcatatgc cgcgtttgca
     2761 taaatttgtc acagatgcag aacctgttga aatgacgagt gacatgaggg ggaataccta
     2821 ttccgataaa gaaaaatatc ccgagcgtgt ccgcatcggc tctggacaac aatggcaaag
     2881 aacggatgaa caacaagcga agggggacgc ttatagctca tggcttgctg gcgcttataa
     2941 ttggagaata gcaggtaaca cacattcaca aagtggagcg ggcaacggca cggtaaactt
     3001 aagtggagat atcacaaaat ctaataatta tggaccttta ccaactgcgg gatctttcgg
     3061 cgatagtgga tcgccaatgt ttatttatga tgcaataaaa caaaaatggc ttattaatgg
     3121 cgtattgcaa actggtaacc cttatttggg ggcgggaaat ggattccaat taattagaaa
     3181 agattggttc tatggcaatg tctttgcaga agatttgcct ataacatttt tagaaccaag
     3241 aagtaacggt cattattcat tttcttcaaa taataatgga actggtacgg ttactcaaac
     3301 gaatgaaaaa gtgagtatgc ctcgatttaa agtcagaacg gttcagttat ttaatgaagc
     3361 attaaaagca gaagataaag agcctgttta tgctgcaggt ggtgtaaatg cttataaacc
     3421 aagactaaat aatggtaaaa atatttcatt tattgactat gcaaaaggcg aagtcacttt
     3481 tactaataat attaatcaag gtgctggtgg tttgtatttt gaaggtgact ttacggtatc
     3541 ttcagaaaat aatgcaactt ggcaaggcgc gggcgttcat atcagtgaag acagtaccgt
     3601 tacttggaaa gtaaacggcg tggcaaacga ccgcctgtcc aaaatcggca aaggcacgct
     3661 gcacgttcaa gccaaagggg aaaaccaagg ctcgatcagc gtgggcgacg gtaaagttat
     3721 tttagatcaa caagcagatg aaaataataa aaaacaagcc tttagtgaaa tcggcttggt
     3781 cagcggcagg gggacggtgc aactgaatgc cgataatcag ttcaaccccg acaaactcta
     3841 tttcggcttt cgcggcggtc gtttggattt gaacgggcat tcgctttcgt tccaccgtat
     3901 tcaaaatacc gatgaagggg cgatgattgt caaccacaat caagacaaag aatccaccgt
     3961 taccattaca ggcaataaag atattactac aaccggcaat aacaacagct tggatagcaa
     4021 aaaagaaatt gcctacaacg gttggtttgg agagaaagat tcaaccaaaa cgaacggtcg
     4081 gctcaacctt gtttaccagc ccgccgcaga agaccgcacc ctgctgcttt ccggcggaac
     4141 aaatttaaac ggcaacatca cccaaacaaa cggcaaactg tttttcagcg gcagaccgac
     4201 accgcacgcc tacaatcatt taaacgacca ttggtcgcaa aaagagggca ttcctcgcgg
     4261 ggaaatcgtg tgggacaacg actggatcga ccgcacattt aaagcggaaa atttcctgat
     4321 taaaggcgga caggcggtgg tttcccgcaa tgttgccaaa gtagaaggcg attggcattt
     4381 gagcaatcac gcccaagcag ttttcggtgt cgcaccgcat caaagccaca caatctgtac
     4441 acgttcggac tggacgggtc tgacaaattg tgtcgaaaaa accattaccg acgaaaaagt
     4501 gattgcttca ttgaccaata ccgacatcag cggcaatgtc agccttgcca atcacgctca
     4561 tttaaatctc acagggcttg ccacactcaa cggcaatctg accgcaggcg gagacacgca
     4621 ctatgaggtt acgcacaacg ccacccaaaa cggcaacctt agcctcgtgg gcaatgccca
     4681 agcaacattt aatcaagcca cattaaacgg caacacatcg gcttcgggca atgcttcatt
     4741 taatctaagc gaccacgccg cacaaaacgg cgatctgacg ctttccggca acgctaaggc
     4801 aaacgtaagc cattccgcat tcaacggcaa tgtctcccta gccgataagg cagtattcca
     4861 ttttgaaaac agccgcctta ccggacaaat cagcggcggc aaggatacgg cattacactt
     4921 aaaagacagc gaatggacgc tgccgtcagg cacggaatta ggcaatttaa accttgacaa
     4981 cgccaccatt acactcaatt ccgcctatcg ccacgatgcg gcaggggcgc aaaccggcag
     5041 tgcgacagat gcgccgcgcc gccgttcgcg ccgttcccta ttatccgtta caccgccaac
     5101 ttcggcagaa tcccgtttca acacgctgac ggtaaacggc aaattgaacg gtcagggaac
     5161 attccgcttt atgtcggaac tcttcggcta ccgcagcgac aaattgaagc tggcggaaag
     5221 ttccgaaggc acttacacct tggcggtcaa caataccggc aacgaacccg taagcctcga
     5281 tcaattgacg gtagtggaag ggaaagacaa caaaccgctg tccgaaaacc ttaatttcac
     5341 cctgcaaaac gaacacgtcg atgccggcgc gtggcgttac caactcatcc gcaaagacgg
     5401 cgagttccgc ctgcataatc cggtcaaaga acaagagctt tccgacaaac ttggcaaggc
     5461 agaagccaaa aaacaggcgg gaaaagacaa cgcgcaaagc cttgacgcgc tgattgcggc
     5521 cgggcgcgat gccgtcgaaa agacagaaag cgttgccgaa ccggcccggc agggaggcgg
     5581 ggaaaatgtc ggcattatgc aggcggagga agagaaaaaa cgggtgcagg cggataaaga
     5641 cagcgccttg gcgaaacagc gcgaagcgga aacccggccg gctaccaccg ccttcccccg
     5701 cgcccgccgc gcccgccggg atttgccgca accccaaccg caaccccaac cgcagcgcga
     5761 cctgatcagc cgttatacca atagcggttt gagtgaattt tctgccacgc tcaacagcgt
     5821 tttcgccgta caggacgaat tagaccgcgt atttgccgaa gaccgccgca acgccgtttg
     5881 gacaagcggc atccgggaca ccaaacacta ccgttcgcaa gatttccgcg cctaccgcca
     5941 acaaaccgac ctgcgccaaa tcggtatgca gaaaaacctc ggcagcgggc gcgtcggcat
     6001 cctgttttcg cacaaccgga ccgaaaacac cttcgacgac ggcatcggca actcggcacg
     6061 gcttgcccac ggcgccgttt tcgggcaata cggcatcggc aggttcgaca tcggcatcag
     6121 cacgggcgcg ggttttagca gcggcagtct ttcagacgac atcggaagca aaatccgccg
     6181 ccgcgtgctg cattacggca ttcaggcacg ataccgcgcc ggtttcggcg gattcggcat
     6241 cgaaccgcac atcggcgcaa cgcgctattt cgtccaaaaa gcggattacc gctacgaaaa
     6301 cgtcaatatc gccacccccg gccttgcgtt caaccgctac cgcgcgggca ttaaggcaga
     6361 ttattcattc aaaccggcgc aacacatttc catcacgcct tatttgagcc tgtcctatac
     6421 cgatgccgct tcgggcaaag tccgaacgcg tgtcaatacc gccgtattgg ctcaggattt
     6481 cggcaaaacc cgcagtgcgg aatggggcgt aaacgccgaa atcaaaggtt tcacgctgtc
     6541 cctccacgct gccgccgcca aaggcccgca actggaagcg caacacagcg cgggcatcaa
     6601 attaggctac cgctggtaac accggatatg ccgaaagggg tttgacgatg ccgccgtgcg
     6661 ctgtcaaacc ctttttctgc cgccgccttg tgcctgctcc attgtctgat aaccgtcaaa
     6721 ttgattttta gcccatgttt ggtgggtcgg ggaaatctat atcttcgtcc gcgccgaaat
     6781 agtctatgcc cgatatacaa ttttgataca caaacttgga aatatcggta tcgtcacc
//
LOCUS       DAXHSF010000109         6783 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.109, whole genome shotgun sequence.
ACCESSION   DAXHSF010000109 DAXHSF010000000
VERSION     DAXHSF010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6783
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.109"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(109..1242)
                     /gene="nrdB"
                     /locus_tag="ACFM8S_001509"
     CDS             complement(109..1242)
                     /gene="nrdB"
                     /locus_tag="ACFM8S_001509"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFC6314568.1"
                     /translation="MSYSTFPKTKNDALNEPMFFGQPVNVARYDQQKYEVFEKLIEKQ
                     LSFFWRPEEIDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWIETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDD
                     LIEYTQYYNLLGEGVHNVGGKPVTVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFA
                     FAERELMEGNAKIIKLIARDEALHLTGTQHMLNLMRSGVDDSEMAEIAAELQDECFQL
                     FKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYITNLRMQAVGLPAGFEGANQNPI
                     PWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL"
     gene            complement(1303..2490)
                     /locus_tag="ACFM8S_001510"
     CDS             complement(1303..2490)
                     /locus_tag="ACFM8S_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HFC6314569.1"
                     /translation="MNDLAAQTIQVVNKSDDLVASAIQTANKSIAVYCRYIRPNDVGT
                     TGSHQSGFYIQKHFSDDLFDVVCQKGTNKTISIKINWQDGSVTNSNFKYYGQGTRNEA
                     RITGFGKNFEFLSDKYSGSLLVLCRACYKDLLFHAFVLSSDEDIEIFIAETNILPGSL
                     YLPKKQEVEDNLTKLFSLFPNFPKTEEMAVLARENIALNKTNVSNVLKQWVSKEYELF
                     SIFEKREFEIIKSKITDLDSFINFAHSFTNRRKARAGKSLELHLSRIFDEFSLKFETQ
                     AKTEGKKKPDFLFPGSEEYHAMDESGNFIFQTEKLTMLGSKTTCKDRWRQVLNEADRI
                     PHKHLFTLQEGISDTQIQEMSDENLTLVVPKESVKTFGTFGKTHVLTLENFIKYIKSQ
                     QVS"
     gene            complement(2483..3496)
                     /gene="dcm"
                     /locus_tag="ACFM8S_001511"
     CDS             complement(2483..3496)
                     /gene="dcm"
                     /locus_tag="ACFM8S_001511"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HFC6314570.1"
                     /translation="MKNKIQNQLDNIVTLNNEKFTFIDLFAGIGGFRIAMENVGGRCV
                     FSSEWDDKARQTYQVNFNDIPYGDITLKETKAAIPSKFDVLTAGFPCQPFSIAGVSKK
                     KSLGRETGFLDKAQGTLFFDVAEIIGKHRPKIFLLENVKNLVSHDKGNTFKVIKGTLE
                     ELDYQIFYQVMNAKYYVPQNRERIFIVGFDRQYFNKEINFNFPSPPESQPKLKQILED
                     DVDNSFTLSDNLWLYLQNYAKKHKAKGNGFGFGLVDLDGISRTLSARYYKDGSEILIP
                     QKGKNPRKLTPRECSRLMGFPKDFVIDAVSKTAAYKQFGNSIAVPLVQAIAKQIINEL
                     KNE"
     gene            complement(3554..5833)
                     /gene="nrdA"
                     /locus_tag="ACFM8S_001512"
     CDS             complement(3554..5833)
                     /gene="nrdA"
                     /locus_tag="ACFM8S_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFC6314571.1"
                     /translation="MNTPTDLKVTKRDGRLEAIDLDKIHRVVTWAADGLENVSVSQVE
                     LKSHIQFYNGIRTDDIHETIIKAAADLISEDTPDYQYLAARLAIFHLRKIAYGEYEPP
                     HLYDHVKKLTDAAKYDRHILEDYSREEFDELNAYIDHERDMSFSYAAVKQLEGKYLVQ
                     NRVTRQIYETPQFLYVLVAMCLFSKYPKETRLDYVKRFYDAVSTFKVSLPTPIMSGVR
                     TPTRQFSSCVLIECDDSLDSINATTSAIVKYVSQRAGIGINAGRIRGLDSEIRGGEAR
                     HTGCIPFFKMFQAAVKSCSQGGVRGGAATLFYPLWHIEAESLLVLKNNRGVEDNRIRQ
                     LDYGVQINRLLYTRLIKGGNITLFSPNEVPGLYEAFFADQDEFERLYTKYEQDPDIRK
                     RIIPAADLFSTLMQERAGTGRIYIQNVDHCNTHSPFDPRVAPVHQSNLCMEIALPTKP
                     LDNINDPNGEIALCTLSAFNLGALNSLDELEGLADLTVRALDALLDYQGYPVEAARTS
                     TMGRRSLGIGVINYAYYLAKNGVRYSDGSALGLTHRTFEAIQYYLLKASANLAKEYGA
                     CTLFNQTVYSQGKLPIDTYKKDLDAVCGEPLHYDWESLRAEIVKYGLRNSTLTALMPS
                     ETSSQIANATNGIEPPRGLVTVKASKDGILKQVVPEFETLKNAYETLWQLPGNEGYLK
                     LVGVMQKFVDQSISANTAYDPGKFEGGKVSMKQMLKDLLTAYKYGVKTLYYHNTRDGA
                     DDTQTDIQDDGCAGGACKI"
     gene            6362..6673
                     /locus_tag="ACFM8S_001513"
     CDS             6362..6673
                     /locus_tag="ACFM8S_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein"
                     /protein_id="HFC6314572.1"
                     /translation="MLAILLSAILGLVSTTAAAGTSEPAHRDTKHIRKANKQMLHPEC
                     RKYLERRAAWYRSQGNVQELRENKKARKAFRSLPYAEQKIQCRAAYEAFDDFDGGSFR
                     R"
BASE COUNT         1809 a         1513 c         1446 g         2015 t
ORIGIN      
        1 cactataaaa ggaacaataa ccgatcctac ccgctgtttt tcccatcata caacatacaa
       61 atgccgtctg aaacatccgg cttcagacgg cattttttca aaaaacattt acaactcaaa
      121 atcgcccaaa tcatccgtat tcacttcaga atcgatctga ccgatcaaat aagaggatat
      181 ttccacttcc tgcggcgcga cctgtacgtt gtcggacgac agccacgcat taatccacgg
      241 aatcgggttt tgatttgcgc cttcaaatcc ggccggcaac cccaccgcct gcatacgcag
      301 attggtaata tattcgacgt attgggataa gatttctttg ttcaaaccaa tcatcgaacc
      361 gtctttaaac aaatatgccg cccattcttt ttcctgttcc gccgcttttt tgaagagttg
      421 gaaacattcg tcctgcaact cggcggcaat ttctgccatt tcagaatcat cgacacccga
      481 acgcatcaga ttaagcatat gctgcgtgcc ggtcaggtgc agggcttcgt cgcgggcaat
      541 cagtttgatg attttggcgt tgccttccat caactcgcgc tcggcaaaag caaacgagca
      601 ggcgaatgaa acgtagaaac ggatggcttc caacacattg acgcacatca ggcagagata
      661 gagttttttc ttcaacccgc gcaaagacac ggtaacgggt ttgccgccga cattgtgcac
      721 cccttcgccc aacaggttgt aatactgggt gtattcgatt aagtcatcgt aatagcaggc
      781 aatgtcttcg gcgcgggcgg taatgtattc gttttcgaca atatcatcaa acacgaccga
      841 cggatcattc acaatattgc ggatgatgtg ggtatagctg cgcgagtgga tggtttcgct
      901 gaagctccac gtttcaatcc acgtttccaa ctcgggaatc gaaaccaaag gcagcaaagc
      961 aacattcgga ctgcgccctt ggatggaatc gagcagtgtt tggtatttca gattgctgat
     1021 gaaaatatgt ttttcgtgtt ctggcaggtt ggcgtagtcg atacggtcgc gcgacacgtc
     1081 gatttcttcc ggccgccaaa aaaacgacaa ttgtttttca atcagttttt caaatacctc
     1141 gtatttctgc tggtcataac gggcgacatt aaccggctga ccgaaaaaca tcggctcatt
     1201 cagcgcgtcg tttttggttt tgggaaaggt gctgtatgac atagttaagt gttcgcagga
     1261 cataatctat tctctatatt aattaaaaat gatctgtaag ttttaactaa cctgctgaga
     1321 ctttatatat ttaataaaat tttctaaagt taaaacatgg gttttcccaa aagttccaaa
     1381 tgtttttacg gattctttag ggacaactaa ggttaaattc tcatcactca tttcctgaat
     1441 ttgagtatca gatatacctt cttgtagagt aaataaatgc ttatgaggaa ttcgatctgc
     1501 ttcatttaaa acttggcgcc agcgatcctt acaagtagtc tttgaaccta gcattgtcag
     1561 cttttcagtt tgaaatatga aattccctga ttcatccatc gcatggtact cttcacttcc
     1621 aggaaataaa aaatctggtt tctttttccc ttctgtcttc gcttgcgtct caaattttaa
     1681 ggaaaattcg tcaaatattc ttgataaatg taactctaaa gattttcctg ctcttgcttt
     1741 tctgcgattt gtaaaagaat gtgcaaaatt aatgaagcta tccaaatcag taatttttga
     1801 tttaataatt tcaaattcac gtttttcaaa aatggaaaat aattcatatt cctttgaaac
     1861 ccattgtttt aacacattac taacgttagt tttatttaat gcaatatttt cccttgccaa
     1921 aactgccatt tcttcagttt tggggaagtt aggaaatagt gaaaataatt ttgttaggtt
     1981 atcctctact tcttgttttt taggtaaata taaacttcct ggtaaaatgt tagtttctgc
     2041 gataaatatt tcaatatctt catctgaaga taaaacaaaa gcatggaata ataaatcttt
     2101 ataacaagct ctgcacaata ccaataagga accactatat ttatcactta aaaattcaaa
     2161 attcttgcca aaacccgtaa ttctcgcctc atttctcgtg ccttggccgt aatatttaaa
     2221 attgctatta gtgacgcttc catcttgcca attaattttg attgaaatag ttttatttgt
     2281 tcccttttgg caaactacat caaagaggtc gtctgaaaaa tgtttttgaa tataaaatcc
     2341 tgattggtga ctaccagtag taccaacatc attgggacga atataacgac agtatactgc
     2401 aattgattta tttgctgtct gtattgctga agctactaag tcatcggatt tatttactac
     2461 ttgtattgtc tgagctgcca agtcattcat tttttaactc atttataatt tgtttagcaa
     2521 tagcttgaac caacggtaca gcaattgaat tgccaaactg cttgtatgca gctgtcttgg
     2581 atactgcatc aataacaaaa tctttaggaa atcccattaa acgcgagcac tccctaggtg
     2641 tcagcttcct aggatttttt cctttctgag ggatgagtat ttcggaacca tctttgtaat
     2701 atcgtgcaga tagagttcgt gatattccat ctaaatcaac taatccaaaa ccaaatccat
     2761 taccctttgc cttatgtttt ttagcgtaat tttgaaggta aagccataag ttatcagaaa
     2821 gagtaaaaga attatctaca tcatcttcca aaatttgctt taattttggt tgtgattctg
     2881 gtggggaagg aaaattgaaa tttatttcct tattaaaata ttgtctatca aaacctacaa
     2941 taaaaatacg ctccctattt tgaggaacat aatattttgc attcataact tgataaaata
     3001 tctgatagtc aagctcttct aaagtccctt taattacttt aaatgtattt cctttgtcat
     3061 gcgaaacaag gtttttcaca ttctctaaaa gaaaaatttt aggtcgatgt tttccaataa
     3121 tttcagcaac atcaaaaaat agagttccct gcgccttatc taagaagcct gtttctcgtc
     3181 ctaggctttt tttctttgaa acaccagcta tagagaatgg ctgacacggg aatcctgctg
     3241 ttaatacatc aaacttactt ggaatagctg ctttggtttc ctttaatgta atatctccat
     3301 aaggaatatc attaaaattt acttggtagg tttgacgggc tttatcatcc cattcactag
     3361 aaaatacaca tcgcccacca acattctcca ttgcaatgcg aaaaccacct attcccgcaa
     3421 ataaatcaat aaaagtaaat ttctcattat ttaaggtaac tatattatct aattgatttt
     3481 gtattttatt tttcatattt tatatttcag atgatttatt aatctaaagg ccatctgaaa
     3541 actccccctt tcatcaaatc ttacaagccc cgcccgcgca gccgtcatcc tgaatatcgg
     3601 tctgcgtatc gtccgcgccg tcgcgggtgt tatggtagta cagggttttg acgccgtatt
     3661 tgtaggcggt cagcaggtct ttgagcattt gtttcataga aactttgccg ccttcgaatt
     3721 tgcccgggtc gtaggcggta ttggcggaaa tcgattgatc gacgaatttt tgcatcacgc
     3781 cgacaagttt caggtagcct tcgttgccgg gaagctgcca cagggtttca taggcatttt
     3841 tcagggtttc aaactccggc acgacttgtt tcaaaatgcc gtctttcgat gctttgaccg
     3901 ttaccaatcc gcgcggcggc tcgatgccgt tggtggcgtt ggcgatttga gagctggttt
     3961 cagacggcat gagcgcggtc agagtagagt tgcgcaggcc gtatttgacg atttcggcac
     4021 gcaggctttc ccaatcgtaa tgcaaaggct cgccgcagac ggcatccaaa tcttttttgt
     4081 aggtgtcgat gggcagtttg ccttgcgaat aaacggtttg gttaaagagc gtacacgcac
     4141 cgtattcttt ggcaaggttt gccgatgctt tgagcaggta atactgtatg gcttcaaagg
     4201 tacggtgggt cagaccgagc gcggagccgt cgctgtagcg gacaccgttt ttcgccagat
     4261 aataagcata gttgatgacg ccgatgccga gcgaacggcg acccatagta gaggtacgcg
     4321 cggcttctac cggatatccc tgataatcta aaagtgcatc gagtgcgcgc acagtcaaat
     4381 cggcaagccc ttccaattcg tccaggctgt ttaatgcgcc caagttgaag gcggacaggg
     4441 tacacagggc gatttcgccg ttcggatcgt tgatattgtc cagcggtttg gtcggcaggg
     4501 cgatttccat acacaagttg gactgatgaa caggcgcgac gcgcggatcg aacgggctgt
     4561 gcgtattgca gtgatcgacg ttttgaatgt agatgcgccc ggttccggca cgctcctgca
     4621 tcagcgtgga aaacaggtcg gcagccggaa tgatgcgctt gcggatatca gggtcttgct
     4681 cgtatttcgt atagagccgc tcaaattcgt cttggtcggc aaaaaacgct tcgtacaatc
     4741 ccggaacctc gttgggcgaa aacagcgtaa tgttgccgcc cttaatcagg cgggtgtaca
     4801 gtaggcggtt gatttgcaca ccgtaatcaa gctggcggat acggttgtct tccacgcccc
     4861 ggttgttttt caacaccagc aggctttcgg cttcgatatg ccacaagggg tagaacaagg
     4921 ttgccgcgcc gccgcgcacg ccgccttgcg aacaggattt gaccgccgcc tgaaacattt
     4981 tgaaaaacgg gatgcagccg gtatgccgcg cttcgccgcc ccggatttcg ctgtccaaac
     5041 cgcggatacg tccggcattg atgccgatgc ccgcacgctg ggaaacgtat ttcacaatcg
     5101 cgctggtagt ggcattgatg gaatccaaac tatcgtcgca ttcaatcagc acacagcttg
     5161 agaactggcg cgtaggcgta cgcacgccgc tcataatcgg ggtcggcagc gatactttaa
     5221 atgtggaaac ggcatcgtaa aaccgtttga cgtaatcgag gcgtgtttct ttgggatatt
     5281 tgctgaaaag acacatcgcc accagaacat ataagaactg cggtgtttca taaatctggc
     5341 gggtaacgcg gttctgtacc agatatttgc cttcgagctg tttgacagcg gcataggaaa
     5401 aggacatatc gcgttcgtgg tcgatatagg cgttcagctc gtcaaattct tcgcggctgt
     5461 aatcctcaag gatatgcctg tcgtattttg cggcatcggt aagtttttta acgtggtcgt
     5521 aaaggtgcgg cggctcgtac tcgccgtagg ctattttacg cagatggaaa atcgccaaac
     5581 gcgcggcaag gtattggtag tccggggtat cttccgaaat taaatcggca gcggctttga
     5641 tgatggtttc gtggatgtcg tcggtgcgga tgccgttgta gaactggatg tgcgatttca
     5701 actcgacctg agatacggaa acattttcca atccgtccgc cgcccaagtg acgacacggt
     5761 gaatcttatc caaatcaatg gcttctaatc ttccgtctcg tttggttact ttcaaatcag
     5821 tcggtgtatt catcgcttcc tcttccactc ttgatattca agacacagtc ttttcaaata
     5881 aattaaggca gacaatatag tggatttttg gcattttctc cagtcttgac aacggttgta
     5941 tttttcagtt tcaggcaatg cgcgataaaa cccgcctgtc gtatttttcc tataatattt
     6001 gttttatctg ataattcttt accgataaaa aacgggtaaa ttttttgcct tttgaccggc
     6061 tccggctaca aggcggtgaa ataaggattt tccgacgaaa aaggaaagct tcctgttttc
     6121 tgcccctgca aattgttaaa ttttgcaaag tatgattttg ccgcgccgcc gccgacaaat
     6181 tccattttct taccgattgg aatttattat tgagattaat gtgttatttg aatctgcata
     6241 tcaaacggca agttttgtcg gctgaatgag tctgaaactg ccgacaattt gcccgttcca
     6301 tccttccatc ctatactgga aagaatgaca aactgaaagg atcgtcatgt ctgtcaaaaa
     6361 gatgcttgcc atactgttgt ctgcaatatt gggactggta tcgacaactg ccgctgccgg
     6421 tacgtcagaa cccgcccacc gcgataccaa acatatccgc aaggcaaaca agcagatgct
     6481 gcaccccgaa tgcaggaaat atttggaacg ccgtgccgcg tggtaccgat cgcaaggcaa
     6541 cgtgcaggaa ttgcgcgaaa acaaaaaggc gcgcaaagca ttccgctccc tgccttatgc
     6601 ggaacagaaa atccaatgcc gggcggctta tgaggctttc gatgatttcg acggcggcag
     6661 tttccgccgt taatcccata taaaatatgc cgtctgaaca caggttcaga cggcattttc
     6721 catagataca acaaactatc cttacgccat ttcgtttgag gtcctcccgt tgccgcagcc
     6781 tta
//
LOCUS       DAXHSF010000110         6751 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.110, whole genome shotgun sequence.
ACCESSION   DAXHSF010000110 DAXHSF010000000
VERSION     DAXHSF010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6751
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.110"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(110..469)
                     /gene="rplT"
                     /locus_tag="ACFM8S_001514"
     CDS             complement(110..469)
                     /gene="rplT"
                     /locus_tag="ACFM8S_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFC6314573.1"
                     /translation="MPRVKRGVTARARHQKIFALAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRQLWIVRINAGARENGLSYSKFMNGLKRASIEIDRKVLADLAVFD
                     KAAFAQLVEKAKAALAA"
     gene            complement(482..679)
                     /gene="rpmI"
                     /locus_tag="ACFM8S_001515"
     CDS             complement(482..679)
                     /gene="rpmI"
                     /locus_tag="ACFM8S_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFC6314574.1"
                     /translation="MPKMKTKSSAKKRFKVLGNGGVKRAHAFKRHILTKKTTKNKRQL
                     RGTSMVNDRDLASVAKMLPYA"
     gene            complement(826..1347)
                     /gene="infC"
                     /locus_tag="ACFM8S_001516"
     CDS             complement(826..1347)
                     /gene="infC"
                     /locus_tag="ACFM8S_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFC6314575.1"
                     /translation="MIAQEREARINGEITAKEVRLISESGEQLGVVSVREALAMAEGQ
                     DVDLVEISPTAKPPVCKLMDYGKYKYQQAKKRDEAKKNQKQVQIKEIKFRPGTDEGDY
                     QIKMRNINRFLADGDKVKVTLRFRGREMAHQQLGAQLLERVKEDLAEVAQIESFPKME
                     GRQMVMMIAPKKK"
     gene            complement(1365..3278)
                     /gene="thrS"
                     /locus_tag="ACFM8S_001517"
     CDS             complement(1365..3278)
                     /gene="thrS"
                     /locus_tag="ACFM8S_001517"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFC6314576.1"
                     /translation="MLNITLPDGSVRQYESPVTVAQIAASIGAGLAKAAVAGRVNGKL
                     VDACDPIVEDSAVQIITPKDQEGIEIIRHSCAHLVGHAVKQLYPNAKMVIGPVIEEGF
                     YYDIATEKPFTPEDVAAIEARMKELIAQDYDVVKIMTPRAEAIKIFQERGEEYKLRLI
                     DDMPEVEAMGMYHHQEYVDMCRGPHVPNTRFLKNFKLTKLAGAYWRGDSNNEMLQRIY
                     GTAWATKDELKAYIQRIEEAEKRDHRKLGKQLDLFHLQDEAPGMVFWHPKGWALWQVI
                     EQHMRKELNAAGYKEVKTPQIMDKTFWEKSGHWENYKDNMFVTSSEKREYAVKPMNCP
                     GHVQIFNNGLRSYRDLPMRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQI
                     VSEARAFNELLVRIYKQFGFHDVSVKLSLRPEQRAGSDDVWDKAEQGLREALTACGVE
                     WGELPGEGAFYGPKIEYHVRDALGRSWQCGTLQLDFVLPERLDAEYVTENNDRARPVM
                     LHRAILGSLERFIGILIENYAGSFPLWLAPVQLVIMNITENQADYCREVAAKLQAAGF
                     RAELDLRNEKIGYKIRDNSQYRFPYQIVVGDKEKQENKVAVRRKAEDLGSLDLDDFIA
                     QLQQEITDALVNH"
     gene            complement(3332..3408)
                     /locus_tag="ACFM8S_001518"
     tRNA            complement(3332..3408)
                     /locus_tag="ACFM8S_001518"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3372..3374),aa:Val,seq:gac)
     gene            3587..4702
                     /gene="tgt"
                     /locus_tag="ACFM8S_001519"
     CDS             3587..4702
                     /gene="tgt"
                     /locus_tag="ACFM8S_001519"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFC6314577.1"
                     /translation="MLKFTLHKKDGYARRGTLELNHGKIETPVFMPVGTYGSVKAMNP
                     QNLHDIKAQIILGNTYHLWLRPGLEVVEQFGGLHGFIGWDKPILTDSGGFQVFSLSDM
                     RKLTEEGCTFKSPINGDKLFLSPEISMKIQTVLNSDIAMQLDECTPGEATREQARKSL
                     QMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESLKGLEELDFPGLAIGGLSV
                     GEPKPEMYRMLRAVGPILPEHKPHYLMGVGTPEDLVYGVAHGVDMFDCVMPTRNARNG
                     WLFTRFGDLKIKNAKHKLDKRPIDESCTCYACQNFSRAYLHHLHRAGEILGAQLNTIH
                     NLHFYQVIMAEMREAVEQGKFADWQARFHENRARGTD"
     gene            4712..5794
                     /gene="hemH"
                     /locus_tag="ACFM8S_001520"
     CDS             4712..5794
                     /gene="hemH"
                     /locus_tag="ACFM8S_001520"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFC6314578.1"
                     /translation="MPSEAASDGILPRYPTRTIFQKPIMLPFLPEPSLSYTQQNRTAV
                     LLLNLGTPDAPTAQAVRPYLKSFLTDRRVVELPKWLWYPILHGLVLTLRPKKSAHAYE
                     KIWFKEGSPLEVYTARQAAALAKRMPDLIVRHAMTYGNPSVADVLSELKAQGAGRLLV
                     IPMYPQYAASSSGAAVDKVCEQLLLQRNQMSVRTVSRFYDDTGYIDAMKNHILRYWAE
                     HGRGKKLMLSFHGVPQKHYDLGDPYPDECRHTAKLLAEALELTEDQYVVSFQSQFGRA
                     KWVTPSTQDLFGKLPKQGVTELDVFCPGFLADCLETMEEIALMGREQFYEAGGKSYRY
                     IPCLNDNPDWIDALVALAEENLGGWR"
     gene            5985..6407
                     /locus_tag="ACFM8S_001521"
     CDS             5985..6407
                     /locus_tag="ACFM8S_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFC6314579.1"
                     /translation="MNTTRLPTALILGCLCAAASAADNSIMTKGQKVYESNCVACHGK
                     KGEGRGTMFPPLYRSDFIMKKPQVLLHSMVKGINGTIKVNGKTYNGFMPATAISDADI
                     AAVATYIMNAFDNGGGSVTEKDVKQAKNKKKLNRQNAV"
     gene            complement(6450..6740)
                     /locus_tag="ACFM8S_001522"
     CDS             complement(6450..6740)
                     /locus_tag="ACFM8S_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314580.1"
                     /translation="MSKYVTETDIGVREHFDFFGEFVVSPAARSDDLALAYGLRLEAG
                     EEGLSLAELFDKRSDSQEPVECGRIDIGGFMLTAKEVDGDGNIGSMGLKVPR"
BASE COUNT         1728 a         1819 c         1569 g         1635 t
ORIGIN      
        1 aacgcaaatt tatttaaaaa aatagacttt aaagaaagaa aatcaatcac atagaatttc
       61 tgcaaacaaa gaaaaaagga agccgcagct tccttttcaa ttttttggat taagcagcca
      121 aagcagcttt ggctttttca accaattgtg caaaagcggc tttatcgaac acggccaaat
      181 cagccaatac tttgcggtcg atttcgatag aggcgcgttt cagaccgttc ataaatttgc
      241 tgtaagacaa cccgttttca cgcgcacctg cattgatacg gacaatccac aattgacgga
      301 attggcgttt gcgttggcgg cggtcgcggt acgcgtattg accggctttc attaccgcct
      361 gcttggcaac gcggtaaacg tttttacgac ggccgcggta gcctttggct aacgcgaaga
      421 ttttttggtg acgggcacgg gcggtaacac cgcgttttac gcgtggcata ttctaaactc
      481 cttaagcgta gggtaacatt ttagcaacag aagccaaatc gcgatcgttt accatagagg
      541 taccgcgcag ttggcgtttg tttttggtgg tctttttagt caagatgtgg cgtttgaacg
      601 catgagcgcg tttcacaccg ccgttaccca gtactttaaa gcgttttttc gcgctagact
      661 tggttttcat tttaggcatg ggaaaactcc attcgttatc agataaggca ttagggggtt
      721 ttaaaacatc ggtttcaaac acttgaaccc acaataccac ggttttttgc cgcaattgaa
      781 aacttactcc gaagcggcaa tcggagtaag cggagaatta tagctttatt ttttcttcgg
      841 cgcaatcatc atcaccattt ggcgaccttc cattttggga aaggactcga tttgcgccac
      901 ttcagccaaa tcttctttta cacgttccaa aagttgcgcg ccgagttgct ggtgagccat
      961 ttcacggccg cggaaacgca atgtcacttt gactttatcg ccgtcggcaa ggaagcggtt
     1021 gatgttgcgc atcttgattt gataatcgcc ctcatccgta cccggacgga atttgatttc
     1081 cttgatttgc acctgctttt ggtttttctt ggcttcgtcg cgtttcttgg cctgctggta
     1141 tttgtattta ccgtaatcca tcagtttgca cacaggcggt ttagcagttg gggaaatctc
     1201 taccaaatcg acatcctgcc cttcggccat agccaaagct tcacgaactg aaacgacacc
     1261 aagctgttcg cctgactcac tgattaaacg cacttctttg gcggtaattt cgccgttgat
     1321 tcgtgcttcg cgttcttgag cgatgatgaa tactcctata aaaattaatg attgacgagg
     1381 gcatcagtga tttcttgctg caattgcgca atgaaatcat ccaaatccaa agaacccaaa
     1441 tcttctgctt tgcggcgtac cgccactttg ttttcctgct tctccttatc gccgacaacg
     1501 atttgataag ggaaacggta ttggctgttg tcgcggattt tgtaaccgat tttttcgtta
     1561 cgcaaatcca actcggcgcg gaatcctgcc gcctgcaatt tggcagccac ttcccgacaa
     1621 taatctgcct gattttcggt aatattcata attaccaatt gaaccggagc cagccataac
     1681 gggaaagagc ctgcatagtt ctcaatcaga atgccgataa atcgctccaa agaacctaaa
     1741 atggcgcgat gcaacataac aggtcgcgca cggtcattgt tttcggttac atattctgca
     1801 tccaagcgtt ccggcaagac gaaatccagt tgtaatgtac cgcattgcca agaacgaccc
     1861 aaggcatctc tgacatgata ttcgatttta ggcccgtaaa acgcaccctc gcccggcaat
     1921 tcgccccatt ccacgccgca ggcagtcaat gcctcgcgca aaccctgctc tgccttatcc
     1981 cacacgtcat ctgaacctgc acgttgttca gggcgaagag aaagcttgac ggatacatca
     2041 tggaaaccga actgtttgta gatgcgaacc aacaattcat tgaacgcacg ggcctcgctg
     2101 acgatttgat cttcggtaca gaagatatgc gcatcatctt gcacaaaacc gcgaacacgc
     2161 atcagaccat gcaatgcacc gcttggttca ttgcggtggc aagaaccgaa ttccgccaaa
     2221 cgcatcggca aatcgcgata tgagcgcaaa ccgttattaa aaatttgcac atgacccgga
     2281 cagttcatcg gtttaaccgc atattcgcgt ttttccgaac tggttacgaa catattgtct
     2341 ttatagtttt cccagtggcc ggatttttcc cagaaagttt tatccataat ctgaggcgtt
     2401 ttcacctctt tataaccggc agcattcaac tctttacgca tatgctgttc aatcacttgc
     2461 cacaaagccc agcctttagg atgccaaaac accatgcccg gtgcttcgtc ttgcaaatgg
     2521 aacagatcca attgcttgcc aagtttgcgg tggtcgcgct tttcggcttc ttcgatacgt
     2581 tgaatatagg ctttcaactc gtcttttgtc gcccaagccg taccgtatat gcgttgcagc
     2641 atttcattgt tgctgtcgcc gcgccagtat gcgcccgcca acttggtcag tttgaagttt
     2701 ttcaggaaac gggtattcgg aacgtgcggt ccgcggcaca tatcgacata ttcctgatga
     2761 tgatacatcc ccatcgcttc cacttcgggc atatcgtcaa tcaggcgcag tttgtattct
     2821 tcgccgcgct cttgaaaaat tttaatcgcc tccgcacgcg gagtcatgat tttgaccaca
     2881 tcatagtctt gagcaatcaa ttctttcata cgcgcttcaa tggcggcaac atcttccggt
     2941 gtaaacggtt tttccgtggc gatgtcgtaa taaaagccct cttcaatgac ggggccgata
     3001 accatttttg cattaggata gagttgcttg acggcatgcc cgacaagatg ggcgcaggaa
     3061 tggcggatga tttcgatgcc ttcctgatct ttcggagtaa tgatttgaac agcagaatct
     3121 tcaacaatcg ggtcgcacgc atcgaccaat ttgccgttta ccctgcctgc caccgccgcc
     3181 ttcgccaaac cggcaccgat agacgcagca atttgagcca cggtaacggg ggattcgtat
     3241 tggcggactg agccgtccgg caaggtaatg ttcaacatca aaaagctcca aaacattaaa
     3301 aaataacggc ataaatgccg ttatgggaat ttggtaggca cgattggatt cgaaccaacg
     3361 acccccacca tgtcaaggtg gtgctctaac caactgagct acgtgccttc aaagaatttt
     3421 tgcattttat cgggtcgttt taatttttgc aagaggtatg tgcgtttctt tatgcaacat
     3481 tgcggcaaga tttggggatg tcgtctgaaa aaccgtggga aatcatttat aatgccgtct
     3541 tttaaaacat atttcgcccc ctgtcgccca actgacattc aatcttatgc ttaaatttac
     3601 cttacacaaa aaagacggat acgcccgacg cggcacgctg gagttgaacc acggcaaaat
     3661 cgaaacgccg gtatttatgc ctgtcggtac ttacggctcg gttaaggcga tgaacccgca
     3721 aaacctgcac gacatcaagg cgcaaattat tttgggcaac acttatcatt tgtggctgcg
     3781 tccgggcttg gaggtcgtcg aacagtttgg agggcttcac ggctttatcg gctgggacaa
     3841 accgattttg accgactcgg gcggttttca ggttttttct ttgtcggata tgcgcaagct
     3901 gacggaagaa ggctgtacgt ttaaaagccc gattaacggc gacaagctgt ttttatcgcc
     3961 tgaaatttct atgaaaatcc aaacggtttt gaactcggac atcgcgatgc agttggacga
     4021 atgcacgccg ggcgaggcga cgcgcgaaca ggcgcgaaaa tcgttgcaaa tgagcctgcg
     4081 ctgggcggaa cggagcaaaa aagcctttga agatttgaaa aacccgaacg cactgttcgg
     4141 catcgtgcaa ggcgcgatgt atgaagattt gcgcgaagag tcgctgaaag gcttggaaga
     4201 gctcgatttc cccggcttgg ccatcggcgg cttgtccgtt ggcgaaccta agcccgaaat
     4261 gtaccgcatg ttgcgcgccg tcggccctat cctgccggaa cacaaacctc attatttaat
     4321 gggcgtcggt acgcctgaag acttagtgta cggcgtggca cacggcgtgg atatgttcga
     4381 ctgcgtcatg cctactcgca atgcgcgtaa cggctggctg tttacccgtt ttggcgattt
     4441 gaaaatcaaa aacgccaaac acaagctcga caagcgtccg atagacgaaa gctgcacctg
     4501 ctacgcctgc caaaacttca gccgcgccta cctgcaccat ctgcaccgcg ccggcgaaat
     4561 cttgggcgca cagttgaaca ccatccacaa tctgcatttc taccaagtca ttatggcgga
     4621 aatgcgcgaa gccgtcgaac aaggcaaatt tgccgactgg caggcgcgat tccacgaaaa
     4681 ccgcgcacgc ggcacagact gactccctgc aatgccgtct gaagccgctt cagacggcat
     4741 tttgccgcgt taccctaccc gcactatttt tcagaaaccg atcatgctcc cattccttcc
     4801 agaaccctcc ctttcctata cgcaacaaaa ccgtaccgcc gttttgctgt tgaacctcgg
     4861 cacgcccgac gcgccgaccg cgcaggcggt acgaccttat ctgaagtcct ttctgaccga
     4921 ccggcgcgtc gtcgaactgc ccaaatggct gtggtatccc attctgcatg gtctggtact
     4981 gacgctccgc cccaaaaaaa gcgcgcacgc ctatgaaaaa atctggttta aggaaggttc
     5041 gcccttagaa gtttacacag cacgccaagc tgccgcgctt gccaaacgta tgcccgacct
     5101 catcgtccgc cacgccatga cctacggcaa tccttcggtg gcagacgtat tgtcggaact
     5161 gaaggcgcaa ggcgcgggca gactgctggt catcccgatg tacccgcaat atgccgcttc
     5221 cagcagcgga gcggcagtag ataaggtgtg cgaacaatta ctgctgcaac ggaaccaaat
     5281 gagcgtccgc accgtttcgc gcttttatga cgataccggc tacattgatg cgatgaaaaa
     5341 ccacatcctc cgatattggg cggaacacgg acgtgggaaa aaactgatgt tgagttttca
     5401 cggcgtaccg cagaagcact acgacctcgg cgacccctat cccgacgagt gccgccacac
     5461 cgccaaactg cttgccgaag cactggaact gaccgaagac caatatgtcg tatcgttcca
     5521 aagccaattc ggcagggcaa aatgggttac accgagcacg caggatttgt tcggcaaact
     5581 gcccaaacag ggggtaaccg agctggacgt attctgcccg ggctttttgg cagactgttt
     5641 ggaaaccatg gaagaaatcg ccctgatggg gcgggaacag ttttatgaag caggcggcaa
     5701 aagctaccgc tacatcccct gcctcaacga caatcccgac tggatagacg cactcgtcgc
     5761 acttgccgaa gaaaaccttg gcggctggcg ttgaaactgt ttgacccatg tctaaaatgc
     5821 cgtctgaacg ccgtgttcag acggcatttt cccgctactt tagcgtatga tggcatctat
     5881 gctggacaac acatcaagtg caaccttact accgattcgg caagaaaatc tttcacccgc
     5941 tacgcaaaat caacaacaag gtttcaaaca ttaaggaaac accaatgaac acaacccgac
     6001 tgccgaccgc cctcatcttg ggctgcctct gcgccgccgc ttctgccgcc gacaacagca
     6061 tcatgacaaa agggcaaaaa gtgtacgaat ccaactgcgt cgcctgccac ggcaaaaagg
     6121 gcgaaggccg cggaaccatg tttccgccgc tctaccgctc cgacttcatc atgaaaaaac
     6181 cgcaggtgct gctgcacagc atggtcaaag gcatcaacgg tacaatcaaa gtcaacggca
     6241 aaacctacaa cggattcatg cccgccactg ccatcagcga tgcggacatt gccgccgtcg
     6301 ccacttatat catgaacgcc tttgacaacg gcggcggaag cgttaccgaa aaagacgtaa
     6361 aacaggcaaa aaacaaaaaa aaactaaaca gacaaaatgc cgtctgaaac cggcaatccg
     6421 gcttcagacg gcattcaaat caaacctttc taacgcggta ctttcagccc catagacccg
     6481 atattgccgt ccccgtcaac ctccttcgcg gtcagcataa agccgccgat gtcaatacgg
     6541 ccgcactcga ccggctcctg actgtcggaa cgcttatcga aaagctcggc aaggctcaaa
     6601 ccctcttcgc ccgcttccag cctcaaaccg taagcaagtg ccaaatcgtc cgaacgtgct
     6661 gccggcgaaa cgacaaactc gccgaagaaa tcaaaatgct cacggacacc gatatccgtc
     6721 tcggtaacgt atttcgacat tttatagtga a
//
LOCUS       DAXHSF010000111         6721 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.111, whole genome shotgun sequence.
ACCESSION   DAXHSF010000111 DAXHSF010000000
VERSION     DAXHSF010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6721
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.111"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(214..1569)
                     /gene="xseA"
                     /locus_tag="ACFM8S_001523"
     CDS             complement(214..1569)
                     /gene="xseA"
                     /locus_tag="ACFM8S_001523"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFC6314581.1"
                     /translation="MSDFFHSDVLSVSELNAFAKSILENHLAGLWIAGEVSNLTRAAS
                     GHYYFSLKDSRAQVRCAMFKGAAARLAKPLKEGDHIEVAGKISIYETRGEFQITVNEV
                     RLKGLGQLYEAYERLKAQLQAEGAFAAERKKPLPARPQCIGIVTSLAAAALRDVVTTL
                     KRRAPEIPVIVYPTAVQGAGSEFQIAQAIKTASQRAECDVLIVCRGGGSIEDLWAFNE
                     EPVVRAIEACTVPVVSGVGHETDFTLADFVADVRAPTPTGAAELVSPNRQESLHRLAQ
                     AQGRLKTVLEQRYFDASQKLDWLARQIRHPRQKLDEQRTYIHKLAQTLSYSMTQNLRA
                     HAARFERQTQALQHYRPDISVYRQDIDRFQTTLSHAFHQLLTHRRQSLTAQAALLEAV
                     SPQHILERGFSVVKNTRGQVIRNADVLKQGQKLHITFADGETDVRVSKEQGQQDLFDC
                     I"
     gene            1766..2587
                     /gene="nadE"
                     /locus_tag="ACFM8S_001524"
     CDS             1766..2587
                     /gene="nadE"
                     /locus_tag="ACFM8S_001524"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HFC6314582.1"
                     /translation="MMRIFSTGGKMDTQAVITHIVRWLDEYAARANAKGFVVGVSGGI
                     DSAVVSALAARTGRPTLLLDMPIRQHPGQLERARLHIRNLQRQYANVSAQTVDLTDTF
                     QTFEQTVGAHQTAFDSQPLSLANARSRLRMLTLYYYGQIHGLLVTGTGNKIEDFGVGF
                     FTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTELTDEEQMGAS
                     YPELEWAMGVYGTRKPEDFEGRQREVLEIYTRLHRAMRHKINPIPVCRIPPELLG"
     gene            complement(2657..3085)
                     /locus_tag="ACFM8S_001525"
     CDS             complement(2657..3085)
                     /locus_tag="ACFM8S_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003743546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HFC6314583.1"
                     /translation="MHYILQRYHNVHRAQFEELVPLAQKVAQVHADTFPAEIAELLAY
                     MQNELLMHMMKEERMLFPMINQGVGRGATMPISVMMHEHEEHDRAIARLKELTDNFQA
                     PEGACGSWTRLYVLTKEMVDDLNDHIHLENDILFARVLDS"
     gene            3532..3864
                     /gene="trxA"
                     /locus_tag="ACFM8S_001526"
     CDS             3532..3864
                     /gene="trxA"
                     /locus_tag="ACFM8S_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFC6314584.1"
                     /translation="MSSELIVHTGDAAFEQDVLKSDLPVLLDFWAPWCGPCKMIAPIL
                     DDIAAEFEGRLKVVKINIDDNEATPSRFGVRGIPTLMVFKNGEVVATKVGALAKGQLT
                     TFVEASIA"
     gene            complement(3917..4864)
                     /locus_tag="ACFM8S_001527"
     CDS             complement(3917..4864)
                     /locus_tag="ACFM8S_001527"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6314585.1"
                     /translation="MTDITPFANRLGKNIKHLMKWAKRNGIEAWRIYDRDIPQFPFAV
                     DVYGDQIHLQEYDTGWLMQPEEYEAWLAEVLEAVAFVTGFAPEQIRLKRRERQKGLQQ
                     YEKTGKAGEDFVITENGRKFWVNLDKYLDTGLFLDHRNTRKKVGETAAGKRFLNLFSY
                     TGSFTVYAATGGAASSETVDLSNTYLDWARRNFELNGIDTERHKIVRADVFQYLQTAY
                     GEGRQFDLIVMDPPSFSNSKKMSDILDIQRDHKKLIDGAVKLLASDGILYFSNNLRSF
                     VLDDLVSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHKS"
     gene            5192..6580
                     /locus_tag="ACFM8S_001528"
     CDS             5192..6580
                     /locus_tag="ACFM8S_001528"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFC6314586.1"
                     /translation="MSIKFADLNLDKNILSAVSSEGYESPTPIQAQAIPSALEGRDIM
                     ASAQTGSGKTAAFLLPTLQKLTKRSEKPGKGPRALVLTPTRELAAQVEKNALAYAKNM
                     RWFRTVSIVGGASFGYQTRALSKPVDLIVATPGRLMDLMQSGKVDFERLEVLILDEAD
                     RMLDMGFIDDIETIVEATPSDRQTLLFSATWDGAVGKLARKLTKDPEIIEVERVDDQG
                     KIEEQLLYCDDMRHKNRLLDHILRDADIDQCVIFTSTKAMTEVIADELYEKGFAANCL
                     HGDMPQGWRNRTLMDLRKGRCKILVATDVAARGIDVPTITHVINYDLPKQAEDYVHRI
                     GRTGRAGRTGIAITFAEVNEYVKVHKIEKYIGRKLPELTIEGMEPTRKRKPAGGKPKG
                     KGGWGDRKAGGQRGDHKPGKEGFGGKTRGEGFKKEGFKKDGFKKTGEGFKGKRKAGDS
                     FAGKSERRYKDR"
BASE COUNT         1583 a         1817 c         1765 g         1556 t
ORIGIN      
        1 ttccgtacta tttgtactgt ctgcggcttc gtcgccttgt cctgattttt gttaatacac
       61 tatatcgaaa cgtatcaatc gtcattcccg cgcaggcggg aatacggttt tttgaatttc
      121 agtcatttct gataaacccc ggcggctttc cgcttccgga ctcccgcttt tgcgggaatg
      181 acggcggagg gggagcggtt tttccgattg ggtttaaatg caatcgaaca aatcctgctg
      241 cccttgttct ttgcttacgc gcacgtcggt ttcgccgtcg gcgaaggtaa tgtgcagctt
      301 ctgcccctgc ttcaaaacat cggcgttgcg gatgacttgt ccgcgtgtgt ttttgacgac
      361 ggaaaagccg cgctccagaa tgtgctgcgg cgaaacggct tcgagcaatg ctgcttgggc
      421 ggtcaggctt tggcggcggt gggtaagcag ttggtggaag gcgtgcgaca aggtcgtctg
      481 aaagcggtcg atgtcttgcc ggtaaacgga aatatcagga cggtaatgtt gcagggcttg
      541 ggtttggcgt tcgaaacggg cggcgtgggc gcggaggttt tgcgtcatcg agtaagacag
      601 cgtttgcgcc agtttgtgga tgtaggtgcg ctgttcgtcg agtttttggc gcggatgacg
      661 gatttgccgc gccagccagt cgagtttttg gctggcatcg aaatagcgtt gttccaaaac
      721 agttttcaga cggccttgcg cttgggctag acgatgtaac gattcttggc ggttgggact
      781 gaccagctcc gccgcgccgg tcggcgtggg cgcgcgcacg tcggcgacaa aatcggcaag
      841 cgtgaaatcg gtttcgtgac ctacgccgct gacgacggga accgtgcagg cttcgattgc
      901 gcgtacgacc ggttcttcgt taaacgccca caagtcttca atgctgccgc cgccgcgaca
      961 gacaatcaac acgtcgcatt cggcgcgttg cgatgcggtt ttaatcgctt gggcaatctg
     1021 aaactcgctg cccgcgccct gaacggcagt cggataaacg atgacgggaa tttcgggcgc
     1081 gcggcgtttc aaggtggtta caacatcgcg caatgccgcc gccgccagac tggttacgat
     1141 gccgatacat tgcggtcggg cgggcaaagg tttcttgcgt tccgccgcaa acgcgccttc
     1201 cgcctgcaac tgcgccttca accgctcgta ggcttcgtaa agctgcccca agcccttgag
     1261 ccgcacttcg ttcacggtaa tctgaaattc gccccgcgtt tcataaatac tgattttgcc
     1321 cgccacttcg atatggtcgc cttctttcaa aggtttcgcc aaacgcgccg ccgcaccctt
     1381 gaacatcgcg caacgcacct gcgcgcggct gtctttgagc gagaaataat aatgcccgct
     1441 ggcggcacgg gtcaggttgg acacttcgcc ggcaatccac aaaccggcaa ggtggttttc
     1501 caaaatactt ttggcaaatg cattcaactc ggaaacggac aacacgtcag aatgaaaaaa
     1561 atcagacatc gaatcaatca aataataaaa aatatgaata tgttttgaag cccaaggcgg
     1621 catcgggccg cctaaattgt caacaatatt ataacacgcg ccatcttgcc gcccgccttt
     1681 tcccgtatga cttttttaag cggggaatgg gaaaaatatt catcaacctg cctgcaatct
     1741 attcaaattg caccgccggc aggctatgat gcggatattt tcgacaggag gaaaaatgga
     1801 tacgcaggca gttatcacac atattgtccg atggttggac gaatacgccg cccgggcaaa
     1861 tgcaaaaggg tttgtcgtgg gcgtttccgg cggcatcgat tccgccgtcg tctccgcact
     1921 cgccgcccgc accggccgcc ccacgctgct tctggatatg ccgatacgcc aacaccccgg
     1981 ccagcttgag cgggcaaggc tgcacatccg caatctgcaa cggcaatatg ccaatgtaag
     2041 cgcgcaaacg gtcgacctga ccgacacctt ccagaccttt gaacaaaccg tcggtgctca
     2101 tcagacggca tttgacagtc agccgctttc cctcgccaac gccagaagcc gcctacgtat
     2161 gctgaccctg tactactacg ggcagataca cggactgctg gttacgggga caggtaataa
     2221 gattgaagat ttcggcgtgg gcttttttac taaatacggc gacggcggcg tggacatcag
     2281 cccgattgcc gacctgacca aaacgcaggt ttaccggctt gccgaagcat tgggcgtgga
     2341 cgaggcgatt caaaaagccc cgccgaccga cggcctgtgg gatacggaac tcaccgacga
     2401 agaacagatg ggcgcaagct atcccgaact ggagtgggca atgggcgtgt acggcacgcg
     2461 caaacccgaa gattttgaag ggcggcagcg cgaagttcta gaaatctata cgcgacttca
     2521 ccgcgccatg cggcacaaaa tcaacccgat tcccgtatgc cgcattccgc ccgaattgct
     2581 gggctgaaac acggaaatgc cgtctgaaac ggaaaaccgt atttcagacg gcatggaaat
     2641 atccggctcc cgttccttaa gaatcgagta cgcgggcaaa cagaatatcg ttttccaaat
     2701 gaatgtggtc gttcaagtcg tccaccattt ctttggtcaa aacgtaaagc cgtgtccagc
     2761 ttccgcaggc tccttcgggc gcttggaaat tatcggtcag ctctttgagc cgtgcgatgg
     2821 cgcggtcgtg ttcttcatgt tcgtgcatca tcacgctgat gggcatcgtc gcaccccggc
     2881 cgacaccctg attaatcatc ggaaacagca tcctttcctc tttcatcata tgcatcagca
     2941 gttcgttctg catataggca agcagctcgg caatttccgc cggaaaggtg tcggcatgaa
     3001 cttgggccac tttttgcgcc agcggcacca attcttcaaa ttgtgcacgg tgtacattgt
     3061 ggtagcgttg caggatatag tggattaaat ttaaaccagt acgtcgttgc ctcgccttgc
     3121 cgtactattt gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccactat
     3181 atgatcgacg gttgcgccaa agggggcggt ttcccaacgg aaaaatcagt catcgcagtg
     3241 ttccttttat agggtttcgg gtttggtttt gaacattcat actttaagaa tctatttaaa
     3301 cggagcatac accgcccgcg cgtttctgta cagcttcaaa aacattcctt acattttgat
     3361 aataaaagta attttcagaa ataaaatact gtccgaaccg tttttttaga atttgcaaag
     3421 gcgattgggg cggtacagaa aaactattat cccgcccgcc cacttgaaat ttttatgccc
     3481 aagccctatc ctgcacgcta tcgtgccaat cccaaccgaa aaggaaaaat aatgagcagc
     3541 gaattaatcg tacacaccgg cgatgccgct ttcgagcaag atgttttgaa atccgacctc
     3601 cccgtcctgc tggacttttg ggctccgtgg tgcggcccct gcaaaatgat tgccccgatt
     3661 ttggacgaca tcgccgccga atttgaaggc cgtctgaaag tagtcaaaat caacatcgac
     3721 gacaacgaag ccaccccgtc ccgtttcggc gtgcgcggca ttccgaccct gatggtgttt
     3781 aaaaacggcg aagtcgtcgc caccaaagtc ggcgcattgg caaaaggcca gctaaccacc
     3841 tttgtcgaag cctctatcgc ctgataaagc gcaatcgaaa aagccgccgg aagattccgg
     3901 cggctttttc gcacccttaa gatttgtggc ggatttccca gcacctatgg atttttttgt
     3961 tgcggaaatc ttcgggaacg gattgtttgg aaatgtcttt gacggcgtat tgttccgata
     4021 ccaagtcgtc taagacgaag ctgcgcaggt tgttggaaaa gtacaaaatg ccgtctgaag
     4081 cgagcagctt caccgcgccg tcaatcagct ttttgtgatc gcgctgaatg tcgaggatgt
     4141 cggacatttt cttgctgttg gaaaaactgg gcggatccat cacaatgagg tcgaactgcc
     4201 tgccttcccc atatgccgtc tgaagatatt ggaacacgtc ggcgcggacg attttgtgcc
     4261 gttccgtatc gatgccgttc aattcaaaat tgcgtctcgc ccaatcgaga taagtgttcg
     4321 acaaatcgac agtttcgctg gatgccgcgc cgccggtggc ggcatagacg gtgaagctgc
     4381 cagtgtagga aaacaggttt aaaaaacgtt tgcccgccgc cgtttcgccg acttttttgc
     4441 gcgtgtttcg atgatccaaa aaaagccccg tatccaaata cttatcaagg ttaacccaaa
     4501 acttgcggcc gttttcggtg atgacgaaat cttcgcccgc cttgccggtt ttctcgtact
     4561 gctgcaaacc tttttggcgt tcgcggcgtt tgaggcggat ttgttcgggc gcaaaaccgg
     4621 taacgaaggc gacggcttcc aagacttcgg caagccacgc ttcgtattct tcgggctgca
     4681 tcagccagcc ggtatcgtat tcctgaaggt ggatttggtc gccgtaaaca tcgacggcga
     4741 aggggaattg tgggatgtcg cggtcgtaaa tgcgccaggc ttcgatgccg ttgcgtttcg
     4801 cccatttcat aaggtgtttg atgtttttgc ccaagcggtt ggcaaacggt gtgatgtcgg
     4861 tcattggttt caggcggaat aaagtggaaa acggcaattt tactgtaatt aacgcccgat
     4921 tgcttgaccg tttcgggcaa accctatacc atccaccgct tatcttgtca tacgaagcca
     4981 tcgccttcca acctaaaccg cccttacggg cgcgtttctt ctgttgcttt gattttacaa
     5041 agcgtatctg tgcaggttgc cgtcgatgta aaccacaagc aagccgcttg cgacaaccct
     5101 gtaacttcac attccccgta tcgttaccct tccctgcttc aggccgtctg aacctttcgg
     5161 acgcgggcgt tgttgtcttc caaggatagc catgtctatt aaatttgccg atttgaacct
     5221 cgataaaaac atcttgtccg ccgtcagcag cgagggttac gaaagcccga ccccgattca
     5281 ggcgcaggcg attccgtccg ccttggaagg tcgcgacatt atggcttcgg cgcaaaccgg
     5341 ctccggcaaa accgccgcct ttctgttacc gactttgcaa aaactgacca aacgcagcga
     5401 aaaaccgggc aaaggcccgc gtgctttggt gttgaccccg acccgcgaac tggcggctca
     5461 agtcgagaaa aacgcgctgg cgtatgccaa aaatatgcgt tggttccgca ccgtcagcat
     5521 cgtcggcggc gcgtctttcg gctaccaaac ccgtgccctg agcaaaccgg tcgatctgat
     5581 tgtcgccacg ccgggccgtc tgatggacct gatgcaaagc ggcaaagttg attttgaacg
     5641 tttggaagtg ctgattttgg acgaagccga ccgtatgttg gatatgggct ttatcgacga
     5701 catcgaaacc atcgtggaag caacgccgag cgaccgtcag actttgttgt tctccgccac
     5761 ttgggacggc gcggtcggca aactggcgcg caaactgacc aaagaccctg aaatcatcga
     5821 agtcgaacgc gtggacgatc aaggcaaaat cgaagaacaa ctgctgtact gcgacgatat
     5881 gcgtcacaaa aaccgcctgc tcgaccacat cctgcgcgat gccgacatcg accaatgcgt
     5941 gattttcacg tccaccaaag cgatgaccga agtcatcgcg gatgaattgt acgaaaaagg
     6001 tttcgccgcc aactgcctgc acggcgacat gccgcaaggc tggcgcaacc gcacgctgat
     6061 ggatttgcgc aaaggccgct gcaaaatttt ggttgccacc gacgttgccg cacgcggtat
     6121 cgacgtaccg accattaccc acgttatcaa ctacgacctg ccgaaacagg cggaagacta
     6181 cgttcaccgc atcggccgta ccggtcgggc aggacgcacg ggtattgcga ttacttttgc
     6241 cgaagtgaac gaatacgtca aagtccacaa aatcgaaaaa tacatcggcc gcaaactgcc
     6301 cgaactgacc atcgaaggca tggaaccgac acgcaaacgc aaaccggccg gcggcaaacc
     6361 gaaaggcaaa ggcggctggg gcgatcgtaa agccggcggc cagcgcggcg atcataaacc
     6421 gggcaaagaa ggcttcggcg gcaaaacgcg cggcgaaggt ttcaagaaag aaggctttaa
     6481 gaaagacggt ttcaaaaaaa ccggcgaagg cttcaaaggc aaacgcaaag ccggcgattc
     6541 ttttgcaggc aaaagcgaac gccgttacaa agaccgctaa gccccaacct gcctcataaa
     6601 ccaatgccgt ctgaaaccga tttcgagttt cagacggcat ttttgcaatg tttcaacacc
     6661 gcccggcttt gatacccaaa ggattaatct gcaatatagt ggattaacaa aaaccagtac
     6721 c
//
LOCUS       DAXHSF010000112         6614 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.112, whole genome shotgun sequence.
ACCESSION   DAXHSF010000112 DAXHSF010000000
VERSION     DAXHSF010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6614
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.112"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            120..1025
                     /gene="glyQ"
                     /locus_tag="ACFM8S_001529"
     CDS             120..1025
                     /gene="glyQ"
                     /locus_tag="ACFM8S_001529"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFC6314587.1"
                     /translation="MLTFQQIIFKLQTFWADKGCTVIQPFDMEVGAGTSHPATCLRAL
                     GPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPANIQDLYLDSLRELGI
                     DPKVHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITY
                     GIERLAMYLQGVENVYDLVWAKTPDGNTVSYGDVYHQNEVEQSTYNFEYSDADWLLRQ
                     FNDYEAQAKRLLAEENAALALPAYELVLKAGHTFNLLDARGAISVTERATYIGRIRAL
                     SRAVAQKYVESREKLGFPLMKANAA"
     gene            1102..1512
                     /locus_tag="ACFM8S_001530"
     CDS             1102..1512
                     /locus_tag="ACFM8S_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding protein YggL"
                     /protein_id="HFC6314588.1"
                     /translation="MPVYFRTPSRKRLHKMNRRQHKKLHLGEFQTLIFGVQGSLHQAE
                     DDAEAFDRFMDELIAYLEVNDFGMAGGGDCGDFSCLFQKSGARSLGETEKKAIISWLA
                     GREDVATLHTTPLVDGFYGDDGWENYSETYKQAD"
     gene            1533..3596
                     /gene="glyS"
                     /locus_tag="ACFM8S_001531"
     CDS             1533..3596
                     /gene="glyS"
                     /locus_tag="ACFM8S_001531"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFC6314589.1"
                     /translation="MMTQTLLIELLTEELPPKALNNLGNHFAAAVAEGLEKAQLVDGA
                     AEFTAYASPRRLAVQVKNVKAVQADQKIVKKGPAVANAMKDGAPTKALEGFARGAGAK
                     IEDLTIVHDGKQDVYAYEYVQTGKPLGELLEDIINQAVKKLPIPKVMRWGSSTFTFVR
                     PVHGLIVLHGGDIVNVSVLGLQSSNQTLGHRFLSNGEITIENADSYAEQMRGQGKVVA
                     SFAERKAAIQTALEGQARSLNAAVAADEALLDEVTALVEWPVVLEAGFEEHFLAVPQE
                     CLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTEDPSHIIRGNERVLRARLSDAEFF
                     YKQDQKATLESRLPKLSSVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAERAAR
                     LAKADLVTEMVGEFPELQGTMGKYYARLDGETEEIAEAIEQHYQPRFAGDKLPESKIA
                     AAVALADKLETLVGIWGIGLIPTGDKDPYALRRAALGILRMLMQYGLDVNELIQTAFD
                     SFPKGLLNEKTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDLTAKLQAVA
                     AFKQLPEAAALAAANKRVQNLLKKADAALGAVNESLLQQDEEKALFAAAQGLQPKIAA
                     AVAEGNFQTALSELASVKPQVDAFFDGVMVMAEDAAVKQNRLNLLNRLAEQMNAVADI
                     ALLGE"
     gene            3613..4659
                     /locus_tag="ACFM8S_001532"
     CDS             3613..4659
                     /locus_tag="ACFM8S_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC6314590.1"
                     /translation="MPSEAFRRHRAYRENKLQPLVSVLICAYNVEKYFAQSLAAVVNQ
                     TWRNLDILIVDDGSTDGTLAIARRFQEQDGRIRILAQPRNSGLIPSLNIGLDELAKSG
                     MGEYIARTDADDIAAPDWIEKIVGEMEKDRSIIAMGAWLEVLSEEKDGNRLARHHRHG
                     KIWKKPTRHEDIADFFPFGNPIHNNTMIMRRSVIDGGLRYNTERDWAEDYQFWYDVSK
                     LGRLAYYPEALVKYRLHANQVSSKYSVRQHEIAQGIQKTARNDFLQSMGFKTRFDSLE
                     YRQIKAVAYELLEKHLPEEDFERARRFLYQCFKRTDTLPAGAWLDFAADGRMRRLFTL
                     RQYFGILHRLLKNR"
     gene            4701..5528
                     /locus_tag="ACFM8S_001533"
     CDS             4701..5528
                     /locus_tag="ACFM8S_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HFC6314591.1"
                     /translation="MQNHVISLASAAERRAHITDTFGVRGIPFQFFDALMPSERLEQV
                     MAELVPGLSAHPYLSGVEKACFMSHAVLWKQALDEGLPYITVFEDDVLLGEGAEKFLA
                     EDAWLQERFDPDSAFIVRLETMFMHVLTSPSGVADYGGRAFPLLESEHWGTAGYIISK
                     KAIRFFLDRFAALPPEGLHPVDLMMFSDFFDREGVPVCQLNPALCAQELHYAKFHDQN
                     SALGSLIEHDRLLNRKQQWRDSPANTFKRRLIRALTKISREREKRRQRREQFIVPFQ"
     gene            5540..5635
                     /locus_tag="ACFM8S_001534"
                     /pseudo
     CDS             5540..5635
                     /locus_tag="ACFM8S_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
     gene            5677..6483
                     /locus_tag="ACFM8S_001535"
     CDS             5677..6483
                     /locus_tag="ACFM8S_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HFC6314592.1"
                     /translation="MQNHVISLASAAERRAHIAATFGARGIPFQFFDALMPSERLEQA
                     MAELVPGLSAHPYLSSVEKACFMSHVVLWKQALDEGVPYVAVFEDDVLLGEGAEKFLD
                     EDAWLQERFDKDSAFIVRLETMFMHVLTSPSGVADYCGRAFPLLESEHWGTAGYIISR
                     KAMRFFLDRFAVLPSERLKAVDWMLFSSFLDKGGMTVCQLTPALCVQSETLPSQLKNG
                     RQESYRNRRSPKVLLKRALGKIGREIERARERKRQKKLEKHLGRHVVPFE"
BASE COUNT         1652 a         1835 c         1750 g         1377 t
ORIGIN      
        1 aatttaatcc actatatcat gtatgccgtg ttttcaaatc agtctgtctt tgagataaac
       61 ctatccaaac agcaatggaa acagtaaaat aaacccttta ttttatcgaa tcataagcca
      121 tgcttacctt ccaacaaatc atcttcaaac tgcaaacctt ctgggcagac aaaggctgca
      181 ccgtcatcca acccttcgac atggaagtcg gtgccggcac atcccatccc gccacctgcc
      241 tgcgcgcgct cgggcccgag ccttggtttg ccgcctacgt ccaacccagc cgccgcccca
      301 aagacggccg ctacggcgac aaccccaacc gcctgcaaca ctattaccaa ttccaagtcg
      361 ccctcaaacc cgcccccgcc aatatccaag acctttatct cgactccctg cgcgaattgg
      421 gcatcgaccc caaagtgcac gacatccgct tcgtcgaaga cgactgggaa aaccccaccc
      481 tcggcgcatg gggcttgggc tgggaagttt ggctcaacgg catggaagta acccagttta
      541 cctatttcca acaagtcggc ggcatcgact gcacgcccgt actcggcgaa atcacctacg
      601 gcatcgaacg cttggcgatg tacctgcaag gcgtggaaaa cgtctatgac ctcgtttggg
      661 caaaaacgcc ggacggcaac accgtaagct acggcgacgt gtaccaccaa aacgaagtcg
      721 aacaatccac ctacaacttc gaatacagcg atgccgattg gctgctgcgc cagttcaacg
      781 actacgaagc gcaagccaaa cgcctgcttg ccgaagaaaa cgccgccctc gccttgcctg
      841 cctacgagct ggttctcaaa gcggggcata cgttcaatct tttagacgca cgcggcgcga
      901 tttccgtaac cgagcgggca acttatattg gacgtattcg tgcattgagc cgcgccgtgg
      961 cgcaaaaata tgttgaaagc cgcgaaaaac tgggcttccc tttaatgaag gcaaatgctg
     1021 cctaaggggg cgacgatttt ttacgttcgg gcggcacgat aaggcagccg tccgtaaacc
     1081 attatatatt ttaggaaaat catgcctgtc tatttccgca ccccaagcag gaaacgcctg
     1141 cacaaaatga accgccgcca gcataaaaag ctgcatttgg gcgagtttca aacattgatt
     1201 ttcggcgttc aaggcagttt gcaccaagct gaggacgatg ccgaagcgtt cgaccgcttt
     1261 atggacgagc tgattgcgta tcttgaggta aacgactttg gaatggcggg cgggggcgat
     1321 tgcggcgatt tttcgtgcct gtttcaaaaa tccggtgccc gaagcttggg cgaaaccgag
     1381 aaaaaagcca ttatctcttg gctggcaggc cgcgaagatg tcgccacttt gcacaccacc
     1441 ccgctggttg acggttttta cggggatgac ggctgggaaa attacagtga aacctataaa
     1501 caggcagatt aaccaaattt aaagaaagcc gaatgatgac ccaaaccctt ttaatcgaac
     1561 tccttaccga agaactcccg cccaaagcac tgaataatct gggcaaccat tttgccgccg
     1621 ccgttgccga aggcttggaa aaagcgcaac tggttgacgg tgcagccgaa tttacggctt
     1681 atgcctcgcc gcgccgtttg gcggttcaag tcaaaaacgt taaagccgtt caggccgacc
     1741 aaaaaatcgt gaaaaaaggc cctgccgtgg cgaatgccat gaaagacggt gcgccgacca
     1801 aggctttgga aggtttcgca cgcggtgcgg gcgcgaaaat cgaagacttg accatcgtcc
     1861 acgacggcaa acaggatgtg tacgcctacg aatacgtcca aaccggcaaa ccgctgggcg
     1921 aacttttgga agacattatc aatcaagccg ttaagaaact gccgattccg aaagtgatgc
     1981 gttggggcag cagcacgttt accttcgtgc gccctgttca cgggctgatt gtgctgcacg
     2041 gcggcgacat cgtaaacgtc agcgttttgg gtctgcaaag cagcaatcaa accttgggac
     2101 accgcttctt gtccaacggc gaaatcacca ttgaaaacgc cgacagctac gccgaacaaa
     2161 tgcgcgggca aggcaaagtc gtcgcttcgt ttgccgagcg caaagccgcg attcagacgg
     2221 cattggaagg gcaggcgcgc agtctgaacg ccgccgtcgc cgccgatgaa gcattgttgg
     2281 acgaagtaac cgcgctggtc gaatggcctg ttgttttgga agccggtttt gaggaacatt
     2341 tcctcgccgt gccgcaggaa tgcttgattt taacgatgca gcaaaaccaa aaatactttc
     2401 cgctgctcga ccaaaacggc aagctgatga accgcttcct gctggtttcc aacctgcaaa
     2461 ccgaagaccc gtcgcacatc atccgaggca acgaacgcgt tttgcgcgcg cgcctgtctg
     2521 atgccgagtt cttctacaaa caagaccaaa aagcgacttt ggaaagccgc ctgccgaaat
     2581 tgtccagcgt ggtgtaccac aacaaaatcg gctcgcaagc cgaacgcatc gaacgcctgc
     2641 aaagcattgc cgcccacatc gccaaagcat tgggcgcgga tgccgccgct gccgaacgcg
     2701 ccgcgcgtct tgccaaagcc gacttggtaa ccgaaatggt tggcgagttc cccgaactgc
     2761 aaggcacgat gggcaaatac tatgcacgtt tggacggcga aaccgaagaa atcgccgaag
     2821 ccatcgagca gcactatcag ccgcgttttg ccggcgacaa gctgcccgaa agcaaaattg
     2881 ccgccgccgt ggcactggcg gacaagctgg aaaccctggt cggcatttgg ggcatcggtc
     2941 tgattccgac cggcgacaaa gacccctacg ccctgcgccg cgccgccttg ggtattttgc
     3001 ggatgctgat gcagtacggt ttggacgtga acgaactgat tcagacggca ttcgacagct
     3061 tccccaaagg tttgctcaac gaaaaaacgc cgtctgaaac cgccgacttt atgcaggcgc
     3121 gccttgccgt gttgctgcaa aacgattatc cgcaagacat cgtcgccgcc gtactcgcca
     3181 aacagccgcg ccgtttggac gatttgaccg ccaaactgca agccgttgcc gcgttcaaac
     3241 agctgcccga agccgccgcg ctcgccgccg ccaacaaacg cgtgcaaaac ctgctgaaaa
     3301 aagccgatgc cgcgttgggc gcggttaacg aaagcctgtt gcaacaggac gaagaaaaag
     3361 ccctctttgc cgccgcgcaa ggcttgcagc cgaaaatcgc cgccgccgtc gccgaaggca
     3421 atttccaaac cgccttgtcc gaactggctt ccgtcaaacc gcaagttgat gcattctttg
     3481 acggcgtgat ggtaatggcg gaagatgccg ccgtaaaaca aaaccgcctg aacctgctga
     3541 accgcttggc agagcaaatg aacgcggtag ccgacatcgc gcttttgggc gagtaaccgt
     3601 tgtacagtcc aaatgccgtc tgaagccttc agacggcatc gtgcctatcg ggagaataaa
     3661 ttgcagcctt tagtcagcgt attgatttgc gcctacaacg tagaaaaata tttcgcccaa
     3721 tcattagccg ccgtcgtgaa tcagacttgg cgcaacttgg atattttgat tgttgatgac
     3781 ggctcgacgg acggcacact tgccattgcc cggcgtttcc aagaacagga cggccgcatc
     3841 cgcatcctcg cgcagccgcg caattccggc ctgattccct ctttaaacat cgggctggac
     3901 gaattggcaa agtcaggaat gggggaatat attgcacgca ccgatgccga cgatattgcc
     3961 gcccccgact ggattgagaa aatcgtgggc gagatggaaa aagaccgcag catcatcgcg
     4021 atgggcgcgt ggctggaagt tttgtcggaa gaaaaggacg gcaaccggct ggcgcggcat
     4081 cacaggcacg gcaaaatttg gaaaaagccg acccgacacg aagatattgc cgactttttc
     4141 cctttcggca accccataca caacaacacg atgattatga ggcgcagcgt cattgacggc
     4201 ggtttgcgtt acaacaccga gcgggattgg gcggaagatt accaattttg gtacgatgtc
     4261 agcaaattgg gcaggctggc ttattatccc gaagccttgg tcaaataccg ccttcacgcc
     4321 aatcaggttt catccaaata cagcgtccgc caacacgaaa tcgcgcaagg catccaaaaa
     4381 accgccagaa acgatttttt gcagtctatg ggttttaaaa cccggttcga cagccttgaa
     4441 taccgccaaa taaaagcagt agcgtatgaa ttgctggaga aacatttgcc ggaagaagat
     4501 tttgaacgcg cccgccggtt tttgtaccaa tgcttcaaac ggacggacac gctgcccgcc
     4561 ggcgcgtggc tggattttgc ggcagacggc aggatgcggc ggctgtttac cttgaggcaa
     4621 tacttcggca ttttgcaccg attgctgaaa aaccgttgaa aaacgccgct ttatccaaca
     4681 gacaaaaaac aggataaatt atgcaaaacc acgttatcag cttagcttcc gccgcagaac
     4741 gcagggcgca cattaccgat accttcggcg tgcgcggcat cccgtttcag tttttcgacg
     4801 cactgatgcc gtctgaaagg ctggaacagg taatggcgga actcgtaccc ggtttgtcgg
     4861 cgcaccccta tttgagcgga gtggaaaaag cctgctttat gagccacgcc gtattgtgga
     4921 agcaggcatt ggacgaaggt ctgccgtata tcaccgtatt tgaggacgac gttttactcg
     4981 gcgaaggcgc ggagaaattc cttgccgaag acgcttggct gcaagaacgc tttgaccccg
     5041 attccgcctt tatcgtccgc ttggaaacga tgtttatgca cgtcctgacc tcgccctccg
     5101 gcgtggcgga ctacggcggg cgtgcctttc cgctgttgga aagcgaacac tgggggacgg
     5161 cgggctatat catttccaaa aaagcgatac ggtttttcct ggacaggttt gccgccctgc
     5221 cgcccgaagg gctgcacccc gtcgatctga tgatgttcag cgattttttc gacagggaag
     5281 gagtgccggt ttgccagctc aatcccgcct tgtgcgccca agagctgcat tatgccaagt
     5341 ttcacgacca aaacagcgca ttgggcagcc tgatcgaaca cgaccgcctc ctgaaccgca
     5401 aacagcaatg gcgcgattcc cccgccaaca cattcaaacg ccgcctgatc cgcgccttga
     5461 ccaaaatcag cagggaaagg gaaaaacgcc ggcaaaggcg cgaacagttc attgtgcctt
     5521 tccaataaaa ggaggagaga tggacatcgt atttgcagca gacggcagga tgcggcggct
     5581 gtttaccttg aggcaatact tcggcatttt gcgccgattg ctgaaaaacc gttgaaaaac
     5641 gccgctttat ccaacagaca aaaaacagga taaattatgc aaaaccatgt tatcagctta
     5701 gcttccgccg cagaacgcag ggcgcacatt gccgcaacct tcggcgcgcg cggcatcccg
     5761 tttcagtttt tcgacgcact gatgccgtct gaaaggctgg aacaggcaat ggcggaactc
     5821 gtacccggct tgtcggcgca tccttatttg agtagtgtgg aaaaagcctg ctttatgagc
     5881 cacgtcgtat tgtggaagca ggcgttggac gaaggcgtac cgtatgttgc cgtatttgag
     5941 gatgatgtct tactcggcga aggggcggag aaattccttg acgaagacgc ttggctgcaa
     6001 gagcgttttg acaaggattc cgcctttatc gtccgcttgg aaacgatgtt tatgcacgtc
     6061 ctgacctcgc cctccggtgt ggcggactac tgcgggcgtg cctttccgct gttggaaagc
     6121 gaacactggg ggacggcggg ctatatcatt tcccgaaagg cgatgcgttt ctttttggac
     6181 aggtttgccg ttttgccgtc tgaacgcctg aaggcggtgg attggatgct cttcagcagt
     6241 tttctcgaca aaggggggat gacggtttgc caactgaccc ccgccttgtg cgtccaaagc
     6301 gaaaccctgc ccagccaact gaaaaacggc aggcaggaat cgtaccgcaa ccgccgctcc
     6361 ccgaaggttt tgttgaaacg cgctttggga aaaatcggca gggaaatcga aagggcgagg
     6421 gaacggaaac gtcagaaaaa attggaaaaa cacctcgggc ggcacgttgt cccgtttgaa
     6481 taggttttgg cctttcctta tttggcgggt gtgcgctgtc cctaaattta tgtcggggaa
     6541 tccggtttgt tcggtttcac ttgttttcgg gtaagcccta attcgtgatt cccgagaaaa
     6601 cagaaagcca aaaa
//
LOCUS       DAXHSF010000113         6603 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.113, whole genome shotgun sequence.
ACCESSION   DAXHSF010000113 DAXHSF010000000
VERSION     DAXHSF010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6603
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.113"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(<1..410)
                     /locus_tag="ACFM8S_001536"
     CDS             complement(<1..410)
                     /locus_tag="ACFM8S_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC6314593.1"
                     /translation="MKTDIQTELTQALLPHEKVWANEEKTILAKNILLDLVEKTDPTI
                     IGLLLNNDELKRHFFVEVNGVLVFKLQDLRFFLDKHSVNNSYTKYANRIGLTDGNRFL
                     KDNSDIVLDFPFKDCVLNGGQSTEEGEEIYFKRS"
     gene            complement(626..1549)
                     /gene="truB"
                     /locus_tag="ACFM8S_001537"
     CDS             complement(626..1549)
                     /gene="truB"
                     /locus_tag="ACFM8S_001537"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFC6314594.1"
                     /translation="MNTGKPQKRAVNGVLLLDKPEGLSSNTALQKARRLFRAEKAGHT
                     GVLDPLATGLLPVCFGEATKFSQYLLDADKAYTATLKLGEASSTGDAEGEIVATARAD
                     ISLAEFQTACKALTGDIRQVPPMFSALKHEGKPLYEYARKGIVIERKARYITVYAIDI
                     AEFDAPKAVIDVRCSKGTYIRTLSEDIAKHIGTFAHLTALRRTETAGFTIAQSHTLEA
                     LANLNETERDSLLLSCDVLVSHFPQTVLNDYAVHMLRCGQRPRFEEDLPSGTPVRVYT
                     EIGEFVGLAEYQKEICRLKALRLMNTAASAA"
     gene            complement(1582..1953)
                     /gene="rbfA"
                     /locus_tag="ACFM8S_001538"
     CDS             complement(1582..1953)
                     /gene="rbfA"
                     /locus_tag="ACFM8S_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFC6314595.1"
                     /translation="MRKPQRGYARQDRVKEQIMRELAELVRTGLKDPRAGFITVNEVE
                     VTRDYSHATVFYTILNQDAREITEEVLEHARGHLRSELAKRIKLFKIPELHFKYDESL
                     ERGMNLSALIDQVAAEKPVED"
     gene            2143..3387
                     /gene="clpX"
                     /locus_tag="ACFM8S_001539"
     CDS             2143..3387
                     /gene="clpX"
                     /locus_tag="ACFM8S_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFC6314596.1"
                     /translation="MSNENRTCSFCGKSKSHVKHLIEGENAFICDECVSNCIEILHED
                     GNDGTSSESVGQTSEEPGKLPTPAEIVANLNDHVIGQEQAKKALAVSVYNHYKRLRHP
                     KAGANVELSKSNILLIGPTGSGKTLLAQSLARKLDVPFVMADATTLTEAGYVGEDVEQ
                     IITKLLGKCDFDVEKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VASVPPQGGRKHPNQEFINVDTTNILFICGGAFAGLEKVIRQRTEKGGIGFGASVHSK
                     DENADITKLFGIVEPEDLIKFGLIPELIGRLPVIATLEELDEDALINILTEPKNALVK
                     QYQALFGMENVELEFEEGALRSIARQAMERKTGARGLRSIVERCLLDTMYRLPDLKGL
                     KKVVVGKAVIEEGREPELVFES"
     gene            complement(3490..4686)
                     /locus_tag="ACFM8S_001540"
     CDS             complement(3490..4686)
                     /locus_tag="ACFM8S_001540"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HFC6314597.1"
                     /translation="MQNYLTPNFAFAPMIPERASGSRVWDTEGREYIDFSGGIAVNAL
                     GHCHPALADALNTQMHKLWHISNIYTTRPAQELAQKLVKNSFADKVFFCNSGAEANEA
                     ALKLARKYARDRFGGGKSEIVACINSFHGRTLFTVSVGGQPKYSKDYAPLPQGITHVP
                     FNDIAALEAAVGEQTCAVIIEPIQGESGILPATAEYLQTARRLCDRHNALLILDEVQT
                     GMGHTGRLFAYEHYGIVPDILSSAKALGCGFPIGAMLATEKIAAAFQPGTHGSTFGGN
                     PMACAVGSRAFDIINAPETLNHVREQGQKLQTALRSLGEQTGVFKQVRGMGLLLGCVL
                     DEAYRGRASEITAAALKHGVMILVAGADVLRFAPSLLLNDEDMAEGLRRLEHALTEFA
                     ATSDNP"
     gene            5090..5590
                     /locus_tag="ACFM8S_001541"
     CDS             5090..5590
                     /locus_tag="ACFM8S_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314598.1"
                     /translation="MNAMPQTAAQNETLHTCSIIVDDAQDFSPMLLDAGRDDTLISES
                     MIPQIRTVAALIAAERHDFSRSSPAEFTDAADFFAARILVLGVRRFHLDVSLLQILKT
                     ANSRARRFAEKHRLFFTPAQAELSLNKHKNRRLLTVETEHEVENKGNPVANSLAFVRK
                     LHTLPL"
     gene            5675..6229
                     /locus_tag="ACFM8S_001542"
     CDS             5675..6229
                     /locus_tag="ACFM8S_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314599.1"
                     /translation="MKKIIASALIATFALAACQDDTQARLEQQQKQIEALQQQLAQQA
                     DDTVYQLTPEAVKDTIPAEAQANGNNGQPVTGKDGQQYIYDQSTGSWLLQSLVGAAAG
                     AFIGNALANKFTRAGNQDSPVARRARAAYHQSAHPNARTSRDLNTRSLRAKQQAAQAQ
                     RYRPTTRPPANYRRPAMRGFGRRR"
BASE COUNT         1577 a         1725 c         1735 g         1566 t
ORIGIN      
        1 ttactgcgtt taaaataaat ctcttcgcct tcttcggtgc tttgtccgcc attcaatacg
       61 caatctttaa acggaaaatc caacacaata tccgaattgt ctttcaaaaa gcggttgccg
      121 tccgtcaaac caatgcggtt ggcgtatttt gtgtaggaat tattgacgct gtgtttgtcc
      181 aagaaaaaac gtaaatcctg caatttaaac accaaaacac cattcacttc cacaaagaaa
      241 tggcgtttta actcatcatt attcaataac aaaccgataa tggtcgggtc ggttttttcc
      301 accaaatcca acagaatgtt tttggctaaa atggtttttt cttcgttcgc ccatactttt
      361 tcgtgtggta gtagggcttg ggttaattcg gtttgaatgt ctgtcttcat cgttataaat
      421 cctcatcagt cattttaatg tgccattctt gtgtaacggc aaatggtttt gcaaataaac
      481 tctaataaaa tcaattattt gctgtatttg ctgtttccgg ggcgttcgca aagttgcgat
      541 aagcggggcg ggtgtccgag tgtgtcggga ttttgcggaa atatcaacac attattcaaa
      601 cacagcccgt atttttaacc gtcgttcagg cggcggatgc cgccgtgttc atcaggcgca
      661 aggctttcag acggcatatt tccttttgat attctgccaa accgacaaat tctccaattt
      721 cagtgtaaac gcgtaccggc gtgccggaag gtaggtcttc ctcgaaacgc ggacgttgtc
      781 cgcagcggag catatggacg gcataatcgt ttaaaacggt ttggggaaag tgtgaaacca
      841 atacgtcgca ggatagcagc aagctgtcgc gttctgtttc gttcaaattt gccaaggcct
      901 caagcgtgtg gctttgggcg atggtaaagc cggcggtttc ggtgcggcgc aaggcggtca
      961 ggtgggcgaa cgtgccgatg tgtttggcga tgtcttcgct gagggtgcgg atgtaggtgc
     1021 ctttgctgca acgtacgtcg atgacggctt tgggcgcgtc aaattcggca atatcgatgg
     1081 cgtaaacggt aatgtagcgc gctttgcgtt cgatgacgat gcctttgcgg gcgtattcgt
     1141 acagcggttt gccttcgtgc ttgagcgcgg aaaacattgg cggcacttgg cggatgtcgc
     1201 ccgttaatgc tttgcaggcc gtctgaaatt cggctaagga aatatcggca cgggcggtgg
     1261 caacgatttc gccttcggca tcacccgtgc tgctggcttc gccgagtttc agcgtggcgg
     1321 tgtaggcttt gtcggcatcc agcaggtatt gggagaactt ggtcgcttcg ccgaagcaga
     1381 cgggcaaaag tccggttgcc aaaggatcga gcacgccggt atgtccggct ttttcggcac
     1441 ggaacaaacg ccgcgctttt tgcagcgcgg tgttgctgga aaggccttcg ggtttgtcca
     1501 agagcaaaac accgttgacg gcacgttttt ggggtttgcc tgtattcatg gttcgatgtt
     1561 cggacggcat gggcatatcc gtcagtcttc aaccggtttt tccgccgcta cttggtcgat
     1621 aagggcggac aggttcatac cgcgttccaa ggattcgtcg tatttgaaat gcagctcggg
     1681 gattttgaac agcttgatgc gtttggccaa ttcgctgcgg aggtgtccgc gcgcgtgttc
     1741 cagcacttct tccgtaattt cgcgcgcgtc ttggtttaaa atggtgtaga acacggtggc
     1801 gtggctgtaa tcgcgggtaa cttcgacttc gttgacggta atgaagccgg cgcgcgggtc
     1861 tttcagtccg gtacggacga gttcggcaag ctcgcgcata atttgttctt tgacacggtc
     1921 ttggcgggca tagccgcgtt ggggttttct catgaatatc ctcagatttc agatttggtt
     1981 tcagacggca tttttgccgc cggggcggtt gcgaaagggc gtattatagc ctaatcggcg
     2041 ggattttaag gtaatacatt gataagaacg gatatagtta ttatacttgg caacccgaac
     2101 ggcgcgtgcc gtttctgtaa cgcatacatt gtaaagaaag ctatgtccaa cgaaaaccgt
     2161 acctgttcct tttgcggaaa atccaaatca cacgtcaaac atttaattga gggcgaaaac
     2221 gcctttatct gtgacgaatg cgtctcaaac tgtattgaaa tattgcatga agacggaaat
     2281 gacggcacgt cttcggaaag tgtcggacag acatcggaag agcccggcaa gctgcccacg
     2341 cccgccgaaa tcgtcgccaa cctcaacgac cacgttatcg gtcaggaaca agccaagaaa
     2401 gccttggcgg tgtcggtgta caaccattac aaacgcctgc gccacccgaa agccggagct
     2461 aatgtcgaat tgtcgaaatc caacatcctg cttatcggcc cgaccggatc gggtaaaacc
     2521 ctgctggcgc aatctttggc gcgcaaactg gatgtgccgt tcgtgatggc agatgcaacc
     2581 acgctgaccg aagcaggtta tgtcggcgaa gatgtcgagc aaatcatcac caaactcttg
     2641 ggcaaatgtg atttcgatgt cgaaaaagcc caacgcggca tcgtctatat cgacgaaatc
     2701 gataagattt cacgcaaaag cgacaatccg tccatcacgc gtgacgtgtc cggcgaaggc
     2761 gtgcaacaag ccttgctgaa actgattgaa ggtacggtgg ccagcgttcc gccccaaggc
     2821 ggccgcaagc atccgaatca ggaatttatc aacgtcgata ccaccaacat cctgtttatc
     2881 tgcggcggcg cgtttgcagg cttggaaaaa gtcatccgcc agcgtactga aaaaggcggt
     2941 atcggtttcg gtgcgtctgt tcacagcaag gacgaaaatg ccgacattac caagctgttc
     3001 ggcatcgtcg aaccggaaga tttgattaaa ttcggtctga ttccggaatt aatcggacgt
     3061 ttgcccgtga ttgcaacact agaagaactg gatgaggacg cgctgattaa tattttgacc
     3121 gagccgaaaa atgctttggt caagcagtat caagccttgt tcggtatgga aaatgtcgag
     3181 ttggaatttg aagaaggcgc attgcgttcc atcgcgcgtc aggcaatgga gcgcaaaacc
     3241 ggcgcgcgcg gcctgcgttc catcgtcgaa cgctgcctgc tcgataccat gtaccgcctg
     3301 cctgatttga aaggcttgaa aaaagtggtg gtcggtaagg cagtcatcga agagggcagg
     3361 gaaccggaat tggtgttcga gtcctgatgg caaacaaaac cgcttcggaa cgttgaacca
     3421 cgtttctgaa gcggtttttt gtttctgatg ccgtctgaag ccttcccgcc ttcagacggc
     3481 atttgagttt tacggattgt ctgatgtcgc ggcaaattcc gtcagcgcgt gttccaaacg
     3541 tcgcaaacct tccgccatat cctcatcgtt caacagtagc gaaggcgcga aacgcaatac
     3601 gtccgcaccc gcaaccaaaa tcatcacgcc gtgtttcaag gcggcggcgg tgatttcgga
     3661 tgcgcgtccg cgataggctt cgtccaacac gcagccgagc agcagcccca tgccgcgaac
     3721 ttgcttaaac acgcccgttt gttctcccaa agaccgcaat gccgtctgaa gtttctgccc
     3781 ctgttcacgg acatggttta aagtttccgg cgcgttgatg atgtcgaagg cgcggctgcc
     3841 gaccgcacac gccatcgggt tgccgccgaa agtcgatccg tgcgtgcccg gttggaaggc
     3901 ggcggcaatc ttttctgtcg ccagcatcgc gccgatcgga aagccgcagc ccaaggcttt
     3961 tgccgaactc aaaatatcgg gaacaatgcc gtaatgttca taggcaaata gcctgcccgt
     4021 atgccccatc ccggtttgaa cttcgtccaa aatcaacaac gcattgtgcc ggtcgcacag
     4081 acggcgcgcg gtttgcaaat attccgcagt ggcgggcagg atgccgcttt cgccctgtat
     4141 cggctcgatg atgaccgcgc aggtctgttc gccgacggca gcttccagcg cggcaatatc
     4201 gttgaacgga acgtgcgtaa tgccttgcgg caggggtgca taatccttgc tgtatttcgg
     4261 ctgaccgccg acggacacgg taaacagcgt gcgtccgtgg aaactgttga tacaggcgac
     4321 gatttcgctt tttcctccgc cgaaacggtc gcgcgcgtat ttcctcgcca gcttcaacgc
     4381 cgcctcattc gcttccgcgc ccgagttgca gaaaaaaacc ttgtcggcaa agctgttctt
     4441 caccaatttt tgcgccaatt cctgcgccgg acgtgtcgta tagatattgg aaatgtgcca
     4501 cagcttgtgc atctgcgtgt ttaaagcatc ggcaagggca gggtggcagt gtcccagcgc
     4561 gttaacggcg atgccgcctg aaaagtcaat atattcacgc ccttccgtat cccaaacgcg
     4621 gctgcctgaa gcgcgttcgg gaatcatcgg ggcaaaggcg aaattggggg tcagatagtt
     4681 ttgcataatt gctcctgatt gcggttttgc tcgggattat gcaccgtttt tcaaagcaga
     4741 taaagggaaa ttgccgacaa tatcgggcag cggtaaaaat aaaccgaaat attttcaatc
     4801 ggatagatga aaaatcaaaa aaatttcgat aaaatgcttg aggcgcggca gcgttttgat
     4861 ttagaatctc cccaatattt ttatcttcag acggcatttc atcatgcaag gcaaaaaatt
     4921 tacaaccgct tattgctatt ggtaccacac agacgtgcgg tacttttttg cgcttgcttg
     4981 aaatccatcc catacgcaaa acaggccgct cgaaaacggg gcggcttttt tgttaccttc
     5041 acacaggtcg cccgattcgg caacacacga cagaatcagg aaaatgacca tgaatgcaat
     5101 gccccaaacc gccgcacaaa acgaaaccct gcacacttgt agcattattg tcgatgatgc
     5161 acaggatttt tcccccatgc ttctcgatgc agggcgggac gacacactca tcagcgaatc
     5221 gatgattccg caaatccgca ctgttgccgc cctgatagcc gccgaacggc acgacttcag
     5281 ccgcagcagc cccgccgagt ttaccgatgc cgccgatttt tttgccgcac ggattctcgt
     5341 attgggcgtg cgacgcttcc atctggacgt atcgctgctt cagattttga aaaccgccaa
     5401 cagccgcgcc cgccgctttg ccgaaaaaca ccgcctgttt ttcacacccg cacaagccga
     5461 attgagcctg aacaaacaca aaaaccgccg cctgctgacc gtagaaaccg aacacgaagt
     5521 cgaaaacaaa ggcaacccag tcgccaacag cctcgcattt gtccgcaaac tgcacaccct
     5581 gcccctgtaa acatcacgat ttgtcggcag aatgcttgcc aaaattcccg acggcgatac
     5641 aataaaaccg attttccacc caaacaggac acacatgaaa aaaatcatcg cctccgcgct
     5701 tatcgcaaca ttcgcactcg ccgcctgcca agacgacacg caggcgcggc tcgaacagca
     5761 gcagaaacag attgaagccc tgcaacagca gctcgcacag caggcagacg atacggttta
     5821 ccaactgact cccgaagcag tcaaagacac cattcctgcc gaagcacagg caaacggcaa
     5881 caacgggcaa cccgttaccg gtaaagacgg gcagcagtat atttacgacc aatcgacagg
     5941 aagctggctg ctgcaaagcc tggtcggcgc ggcggcaggc gcgtttatcg gcaacgcgct
     6001 ggcaaacaaa ttcacacggg caggcaacca agacagtccc gtcgcccggc gcgcgcgtgc
     6061 cgcctaccat cagtccgcac atcccaatgc gcgcaccagc agggatttga acacgcgcag
     6121 cctccgtgca aaacaacagg cggcgcaggc gcagcgttac cgcccgacaa cgcgcccgcc
     6181 cgccaattac cgccgaccgg ctatgcgcgg tttcggcagg aggcggtaaa cccggcgcgc
     6241 caatgccgtc tgaagagctt tcagacggca tttttgcatt tgttagggac attgttatgt
     6301 tgccgtttga ttttcagacg gcattttgtt tccaagcgtt tggtgtcggg atggcaattc
     6361 tgatctacgt gcgttggcag ggtggcattg tcatgccgcc gttgggtttt cagacggcat
     6421 catatcgaac cgtcaggtcg tggggtgagc attcttgccc accgttgcaa gggcgggatt
     6481 ttttggtgaa caggaatgcc caccctgcat ctgaaaccct tctgcgtcat tcccgtgcag
     6541 gaaggaatct agatatttga atttacggaa attttcaatc gttgccaaag cggaaaaggg
     6601 ttt
//
LOCUS       DAXHSF010000114         6593 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.114, whole genome shotgun sequence.
ACCESSION   DAXHSF010000114 DAXHSF010000000
VERSION     DAXHSF010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6593
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.114"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(<1..390)
                     /locus_tag="ACFM8S_001543"
     CDS             complement(<1..390)
                     /locus_tag="ACFM8S_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HFC6314600.1"
                     /translation="MKERIVGQSGELFCFGQMPVWKAENLPEILLSGYSSEGGEWVCL
                     NVLQGDVEVRTQDGAAEVWSAESGDCVFAPQQVFSVKLKTDDAEIRLSLYCAAADYFH
                     KKYGMSATHSAVAAARDTVPAGRALDMG"
     gene            504..941
                     /gene="rnhA"
                     /locus_tag="ACFM8S_001544"
     CDS             504..941
                     /gene="rnhA"
                     /locus_tag="ACFM8S_001544"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFC6314601.1"
                     /translation="MDQTVYLYTDGACKGNPGAGGWGVLMRYGSHEKELFGGEAQTTN
                     NRMELTAVIEGLKSLKRHCTVIICTDSQYVKNGMENWIHGWKRNGWKTAAKQPVKNDD
                     LWKELDTLVGQHQVSWAWVKGHAGHAENERADDLANRGAAQFS"
     gene            complement(1014..1427)
                     /locus_tag="ACFM8S_001545"
     CDS             complement(1014..1427)
                     /locus_tag="ACFM8S_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314602.1"
                     /translation="MNKFLATWLAPILVLSGCATIMGESSQTVPIKSQPSGAVFKIQD
                     ETGVIVQSGVTPANVSLPKGNGKYFGRKKYQVSFEKKGYQPLVYKLETNPNGWYIGGN
                     LIFGGLIGYLIVDPLSNGKMFTISPKQVDVPLIAK"
     gene            1579..1932
                     /locus_tag="ACFM8S_001546"
     CDS             1579..1932
                     /locus_tag="ACFM8S_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6314603.1"
                     /translation="MVQLLATEADRLIGEKIRQRRKELGYSAEKLAEHINLSQQQISR
                     YERGVGKINISHLVDIAVFLETPIAWFFQDCLPKPLTVACTPENTPEQQWHSLNPNQK
                     AAFAAFLDTLRNKET"
     gene            1929..2333
                     /locus_tag="ACFM8S_001547"
     CDS             1929..2333
                     /locus_tag="ACFM8S_001547"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFC6314604.1"
                     /translation="MNTKPCPICTAQDEDILLQTPKLRVIAVHNDSGSPAFCRVIWRK
                     HIAEMTDLSAAERGELMEMVYKVEAAMRQVFRPAKINLASLGNVVPHLHWHIIARFEN
                     DASFPAPIWANPVRKHGMTLPQDWTEQLKKLL"
     gene            2373..3557
                     /locus_tag="ACFM8S_001548"
     CDS             2373..3557
                     /locus_tag="ACFM8S_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HFC6314605.1"
                     /translation="MTEPTSRRRFLKTCTAAAGAGLLQACGTSATSVPPLPSSHSVVK
                     ARTVPLQTPRRQSSDGNLLRVVASSGFAEDTNRVNTALTRLYNAGFTVTNQQAGSRRF
                     QRFAGTDTQRAADFQEVASSRVATPKVLMGLRGGYGAARILPHIDFASLGARMLEHGT
                     LFFGFSDVCAVQLALLAKGNMMSFAGPMAYSDFGKPAPGAFTMDAFIKGATQNRLTVD
                     VPYIQRADVETEGILWGGNLSVLASLAGTPYMPDIDGGILFLEDVGEQPYRIERMLNT
                     LYLSGILKKQRAIVFGNFRMEKIRDVYDPSYDFSAVANHVSRTAKIPVLTGFPFGHIA
                     DKITFPLGAHARIRMNGNGGYAVAFEGYPTLDASALTLDTLLPPPDLPIFPESGVADI
                     SE"
     gene            complement(3620..4153)
                     /locus_tag="ACFM8S_001549"
     CDS             complement(3620..4153)
                     /locus_tag="ACFM8S_001549"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFC6314606.1"
                     /translation="MGIYDFQMKDAEGNAVDLSGYRGKVLLIVNTATRCGLTPQYEAL
                     QKLYAQYTAEGLEILDFPCNQFREQAPESSGEIAQVCMMKFGTKFKIFDKIEVNGANT
                     APLYAYLKSVKPQDKGNHLFKDFVLKLAALGEKRDEGDIKWNFTKFLVNRDGEVVERF
                     APSVTPEEIEADIRALL"
     gene            complement(4332..6587)
                     /locus_tag="ACFM8S_001550"
     CDS             complement(4332..6587)
                     /locus_tag="ACFM8S_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HFC6314607.1"
                     /translation="MGQYKKLWYLLFAVLAVCFTILGYMGSEVYKKAPPYPEQVVSAS
                     GKVLMTKDDILAGQSAWQSTGGMEVGSILGHGAYQAPDWTADWLHRELSAWLDLTAQQ
                     TYGKKFDEVSPEEQAVLKTRLADEYHNQSRIKEDGSVVVSETRVKAIESILPYYHGVY
                     SDDPKFQTTREHFAMKNNTLPSQEAREKLFDFFFWTSWSASTNRPDENFTYTNNWPHE
                     PLINNVPTTENYMWSFTSVVLLLMGIGLLMWGYSFLTKHEEVEVPTEDPISKVQLTPS
                     QKALGKYVFLTVALFVVQVLLGGLTAHYTVEGQGFYGIDEALGFEMSDWFPYALTRTW
                     HIQSAIFWIATGFLTAGLFLAPIVNGGKDPKFQRAGVNFLYIALFIVVGGSYAGNFFA
                     LTHILPPEFNFWFGHQGYEYLDLGRFWQLLLMVGLLLWLFLMLRCTVSAFKEKGVDKN
                     LLAIFVASMVGVGVFYAPGLFYGEKSPIAVMEYWRWWVVHLWVEGFFEVFATAAFAFV
                     FYNMGFVRRSTATASTLAAAAIFMLGGVPGTLHHLYFSGSTSASMAIGACFSALEVVP
                     LVLLGREAYEHWSYQHLSDWAKRLRWPLMCFVAVAFWNMIGAGVFGFLINPPISLFYI
                     QGLNTSAVHAHAALFGVYGFLALGFVLLVARYLKPNAQFDDKLMTWGFWLLNGGLVGM
                     IAISLLPVGAIQAYASITHGLWYARSEEFLQMEILDTLRWVRTAADLVFIGGAICVAI
                     QATKIVFGRDK"
BASE COUNT         1745 a         1839 c         1560 g         1449 t
ORIGIN      
        1 acccatatcc aacgccctgc ccgccggtac ggtatcccgt gccgccgcga ccgcagaatg
       61 cgtggcactc atcccgtatt ttttgtgaaa atagtctgcc gccgcgcaat acagcgacaa
      121 acggatttcg gcatcgtccg ttttcagttt gaccgaaaac acctgctgcg gcgcaaacac
      181 gcaatcgccg ctttctgccg accaaacctc cgccgccccg tcctgcgtgc ggacttcgac
      241 atcgccctgc aacacattca ggcagaccca ttcccctcct tcggacgaat aacccgacaa
      301 caaaatttcc ggcaggtttt ccgccttcca tacgggcatt tgtccgaaac aaaacaactc
      361 gccgctttga cccactatcc gctccttcat attcaaaaat aaagttgcac attatatgcc
      421 tattttaatc cgccgcaatc tttcagacgg cacggcgcac aaaccgctta taatcacgcc
      481 ggacaccaca caaaggcaca ataatggacc aaaccgttta cctttacacc gacggcgcgt
      541 gcaaaggcaa tcccggcgcg ggcggctggg gcgtgttaat gcgctacggt agccacgaaa
      601 aagaactttt cggcggcgaa gcgcaaacca ccaacaaccg catggaactg actgccgtca
      661 tcgaaggact gaaatccctc aaacgccact gcaccgtcat catctgcacc gactcgcaat
      721 acgttaaaaa cggcatggaa aactggatac acggttggaa gcgcaacggc tggaaaaccg
      781 ccgccaaaca gcccgtcaaa aacgacgact tgtggaaaga actcgacaca ctggtcggac
      841 agcatcaagt cagctgggct tgggtgaaag gacacgcggg acacgccgaa aacgaacgcg
      901 ccgacgattt ggcaaaccgt ggcgcggcgc agttttcctg agtgccgcac cgacaggaaa
      961 ataccgtctg aaaccgctaa tgggcttcag acggcatttt taccatacac tccttatttc
     1021 gcaattaacg gtacatctac ctgcttgggt gaaatcgtaa acatcttacc gttactcaac
     1081 ggatcaacaa tcagataacc aatcaggcca ccgaaaatca ggtttccacc aatataccaa
     1141 ccattcggat tggtttctaa cttgtaaacc aacggctgat aacctttctt ctcaaaagaa
     1201 acctgatatt ttttcctgcc gaaatatttg ccgttgcctt taggcaacga aacatttgcg
     1261 ggcgttacac ctgattgaac aatcacacct gtttcatcct gaattttaaa taccgcaccg
     1321 gatggttgac ttttaatcgg cacggtctga ctggactctc ccataatcgt tgcacaaccc
     1381 gacaaaacca aaatcggtgc aagccatgtt gccaagaact tattcatcaa aatcatctcc
     1441 aactgactaa aaaataaaat acattaaatt acacaataat gatgtaattt ggaatatttt
     1501 acttatttac aacactgttt acaagagaaa aaacaagata aatatccata gatgagtaaa
     1561 tctacaatcg gatacaaaat ggtacaattg cttgcaacag aagcagaccg gctaataggt
     1621 gaaaaaatcc gacaacgacg aaaagagttg ggctattctg cagaaaagct ggcggaacac
     1681 atcaatcttt ctcaacagca gatttcacgc tacgaacgtg gcgtaggtaa aattaatatt
     1741 tcgcacttag tcgatattgc cgtttttctg gaaacaccga ttgcttggtt tttccaagat
     1801 tgcctgccca aaccactgac ggtagcctgt actcctgaga acacacccga acagcaatgg
     1861 cacagcctta atccaaacca aaaagctgcc tttgccgcct ttttggatac tttaaggaat
     1921 aaagaaacat gaacaccaag ccctgcccca tctgcacggc acaagatgaa gatattttgc
     1981 tgcaaacccc caagctgcgc gtcatcgccg tccataacga cagcggttcg cctgcattct
     2041 gccgcgtcat ttggcgtaag catattgccg aaatgaccga cctttcggca gcggaacgcg
     2101 gcgaattgat ggaaatggtg tacaaagtcg aagccgctat gcgccaagtg ttccggccgg
     2161 caaaaatcaa cctcgccagc ttgggcaatg tcgtgccgca cctgcactgg catattatcg
     2221 cccgctttga aaacgatgcg tctttccccg cgccgatttg ggcaaacccc gtccggaaac
     2281 acggtatgac cctgccgcaa gattggacgg aacagcttaa aaagctgctt taagcccgcc
     2341 gatgccgtct gaaaccgtat gaaaggggaa ttatgaccga accgacctcc cgccgccgtt
     2401 tcctgaaaac ctgcaccgcc gccgccggcg cgggactgct tcaggcttgc ggcacatccg
     2461 ccacatccgt tccgcccctt ccctcttccc attccgttgt gaaagcccga accgtgcctc
     2521 tccaaacgcc acgccgtcaa agttcggacg gcaaccttct gcgcgttgtc gcttcgtcag
     2581 gatttgccga agataccaac cgcgtcaaca cagccttaac ccgcctttac aatgccggtt
     2641 ttaccgtaac caaccaacag gcgggcagcc gccgtttcca acggtttgcc ggtacggaca
     2701 cacaacgtgc cgccgatttc caagaggtcg cttcgagccg cgtcgccacg cctaaagttc
     2761 tgatgggttt gcgcggcggt tacggtgcgg cgcggattct gccgcatatc gattttgctt
     2821 cgctcggcgc aaggatgctc gaacacggca cgctgttttt cggattcagc gacgtatgcg
     2881 ccgtccagct ggcattgttg gcaaaaggca atatgatgag ttttgccggc ccgatggctt
     2941 atagcgattt tggcaaaccc gcccccggtg cgtttacgat ggatgccttt atcaagggtg
     3001 caacccaaaa ccgcctgacc gttgatgttc cttatatcca acgcgccgat gtcgaaaccg
     3061 aaggcatatt gtggggcggc aacttaagcg tcctcgcctc gctcgccggc acgccttata
     3121 tgcccgacat cgacggcggc attttgttcc tcgaagatgt cggcgaacag ccctaccgca
     3181 tcgaacgtat gctcaatacg ctgtatcttt cgggtatttt gaagaaacag cgcgccatcg
     3241 tgttcggcaa tttccgtatg gaaaaaattc gagatgtcta tgatccgtct tatgattttt
     3301 ctgccgttgc caaccatgtt tcgcgcacgg cgaaaatccc cgtgctgacg ggcttcccgt
     3361 tcggacacat tgccgacaaa atcactttcc ctctaggcgc gcacgcccga atccgtatga
     3421 atggaaacgg cggttatgcg gtcgcgtttg aaggctaccc cacactcgat gcgtccgccc
     3481 tgactttgga taccctgctc ccgccgccgg atttgcctat cttccccgaa agcggtgttg
     3541 ccgatatttc ggaataaacc cgcaaacgga caaatgccgt ctgaagcctt cagacggcat
     3601 ttcccaagac ggcggcagat tacagcaatg cccgaatatc ggcttcgatt tcttcgggcg
     3661 taacactagg cgcaaaacgc tcgaccactt cgccgtcgcg gttgacgagg aatttggtaa
     3721 agttccattt gatgtcgcct tcgtcgcgct tctctcccaa agctgcgagc ttcaacacga
     3781 aatctttaaa cagatgattg cctttatctt gcggtttgac ggatttcagg taggcataca
     3841 agggcgcggt atttgctcca ttgacttcga ttttgtcgaa aatcttaaac ttcgtgccaa
     3901 acttcatcat acacacttgg gcaatttctc cgctgctttc gggagcctgt tcgcggaact
     3961 ggttgcacgg aaaatccaaa atctccaagc cttctgcggt atattgtgca tacagcttct
     4021 gcaaagcctc gtattgcggg gtcagaccgc aacgcgttgc cgtgttgaca atcagcagaa
     4081 ccttgccgcg atagcctgac aaatcaaccg cattgccttc cgcatctttc atttgaaaat
     4141 cgtaaatacc catttttatc cttatctgat gtaaaccgat gccatctgaa acgtgcttca
     4201 gacggcatga aagcagcaat tgtatagccg attaaaataa aaaatccaca tccttttcca
     4261 ttcccgtccc aatccgcaat aaaaaactgc acccgaaaac gggtgcagtt gctcatttca
     4321 taccgcaaaa cttatttgtc gcggccgaat acgattttag tggcttggat ggcaacgcag
     4381 attgcaccgc cgataaagac caagtcagct gccgtacgta cccaacgcaa ggtatcgagg
     4441 atttccattt gcaggaactc ttcgctgcgg gcataccaca gaccgtgcgt gatggaggcg
     4501 tatgcctgaa tcgcgccgac aggcagcagg ctgatggcaa tcataccgac caagccgccg
     4561 ttgagcagcc agaaacccca agtcatcaat ttgtcgtcaa actgcgcgtt cggtttcaaa
     4621 taacgggcaa ccagcaatac gaagcccaat gccaagaaac cgtacacacc gaacaaggcg
     4681 gcgtgcgcgt gaacggcaga agtgttcaaa ccttggatat agaacaggga aatcggcgga
     4741 ttgatcagga agccgaatac gccggcaccg atcatattcc aaaaggcgac tgccacgaag
     4801 cacatcagcg gccaacgcag gcgtttcgcc cagtcggaca ggtgttggta agaccagtgt
     4861 tcgtatgctt cacggcccag caacaccagc ggcacgactt ccaaagcgga gaagcaggca
     4921 ccgattgcca tagaggcgga ggtagagccg gagaagtaca ggtggtgcag cgtaccagga
     4981 acgccgccca acataaagat ggcggcagcg gccaaagtgg aggcagtggc ggtactgcgg
     5041 cggacaaagc ccatattgta gaagacaaag gcaaaggcgg cagtggcaaa tacttcgaag
     5101 aagccttcta cccacaggtg aaccacccac caacgccagt attccataac ggcaatcggg
     5161 gatttttcac cgtagaacag acccggcgcg tagaacacgc ccacgccgac catagaagct
     5221 acgaagatag ccaacaggtt tttgtccacg cctttttctt tgaaggcgga aaccgtgcaa
     5281 cgcaacatca ggaacagcca taacagcaga ccgaccatca aaaggagttg ccagaaacgg
     5341 cccaaatcga ggtattcgta accttggtgt ccgaaccaga agttaaattc cgggggaagg
     5401 atgtgcgtca acgcgaagaa gttgcccgcg taagaaccgc cgaccacgat gaagagggcg
     5461 atatagagga agtttacgcc ggcacgttgg aacttgggat ctttaccgcc gttgacaatc
     5521 ggcgcgagga acaaacctgc cgtcaaaaag ccggttgcaa tccagaagat ggcggattgg
     5581 atgtgccaag tacgggtcag ggcgtagggg aaccagtcgg acatttcaaa gcccaacgcc
     5641 tcgtcaatgc cgtagaaacc ctgaccttcg acggtgtagt gcgcggtcag accgcccagc
     5701 aatacctgta cgacaaacag tgcaacagtc agaaatacat atttgcccaa cgctttctga
     5761 gaaggagtca gttgtacttt agagatagga tcttcagtag gtacttcaac ctcttcatgt
     5821 ttggtcaaga aagagtaacc ccacatcagc aagccgatac ccatcagcaa caataccacg
     5881 ctggtgaaag accacatata gttttcagta gtcggcacgt tgttgatcag aggctcatgc
     5941 ggccagttgt tggtgtaggt gaagttctcg tcaggacggt tggtcgaagc agaccaagaa
     6001 gtccagaaga agaagtcgaa cagtttttca cgcgcttctt ggcttggcaa tgtattgttt
     6061 ttcattgcga agtgttcgcg agtggtttga aacttaggat cgtcgctgta cacaccgtgg
     6121 tagtaaggca ggatgctttc gatcgctttc acgcgggttt cgctgacaac gacagaacca
     6181 tcttccttaa tacggctttg gttgtggtat tcgtcagcca aacgggtttt cagaacagct
     6241 tgttcttcag gagaaacttc atcaaatttt ttgccgtaag tctgttgcgc ggtcaaatcc
     6301 aaccaagcgg acaactcacg atgcagccag tcggccgtcc agtccggagc ctgatatgcg
     6361 ccgtgtccca gaatcgaacc gacttccata ccgccggtac tctgccacgc agactgacct
     6421 gccaaaatat cgtctttcgt catcagcact ttgcctgatg cggaaacgac ctgttcgggg
     6481 taaggcgggg ctttcttata aacctcgctg cccatatagc caagaatggt aaagcatacc
     6541 gccagaacgg caaacagcaa gtaccacagc ttcttgtact gtcccatttt gag
//
LOCUS       DAXHSF010000115         6565 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.115, whole genome shotgun sequence.
ACCESSION   DAXHSF010000115 DAXHSF010000000
VERSION     DAXHSF010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6565
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.115"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            335..2164
                     /locus_tag="ACFM8S_001551"
     CDS             335..2164
                     /locus_tag="ACFM8S_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HFC6314608.1"
                     /translation="MMIKPTALLLPALFFFPHAYAPAADLSENKAAGFALFKNKSPDT
                     ESVKLKPKFPVRIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQTGFLAEEAPDNVKTM
                     LRSKGYFSSKVSLTEKDGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEYYRNALE
                     NWQQPVGSDFDQDSWENSKTSVLGAVTRKGYPLAKLGNTRAAVNPDTATVDLNVVVDS
                     GRPIAFGDFEITGTQRYPEQIVSGLARFQPGTPYDLDLLLDFQQALEQNGHYSGASVQ
                     ADFDRLQGDRVPVKVSVTEVKRHKLETGIRLDSEYGLGGKIAYDYYNLFNKGYIGSVV
                     WDMDKYETTLAAGISQPRNYRGNYWTSNVSYNRSTTQNLEKRAFSGGIWYVRDRAGID
                     ARLGAEFLAEGRKIPGSDIDLGNSHATMLTASWKRQLLNNVLHPENGHYLDGKIGTTL
                     GTFLSSTALIRTSARAGYFFTPENKKLGTFIIRGQAGYTLARDNADVPSGLMFRSGGA
                     SSVRGYELDSIGLAGPNGSVLPERALLVGSLEYQLPFTRTLSGAVFHDMGDAAANFKR
                     MKLKHGSGLGVRWFSPLAPFSFDIAYGHSDKKIRWHISLGTRF"
     gene            2219..6388
                     /locus_tag="ACFM8S_001552"
     CDS             2219..6388
                     /locus_tag="ACFM8S_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HFC6314609.1"
                     /translation="MTDTAPTDTDPTENGTRKMPSEHRPTPPAKKRRPLLKLSAALLS
                     VLILAVCFLGWLAGTEAGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEG
                     ADLKISRFRFAWKPSELMRRSLHITEISAGDIAIVTKPTPPKEERPPLSLPDSIDLPA
                     AVYLDLFKTGKISVGKAFDKQTVYLERLDASYRYDRKGHRLDLKAADTPWSSSSGAAS
                     VGLKKPFALDTAIYTKGGLEGKTIHSTARLSGSLKDVRAELAIDGGNIRLSGKSVIHP
                     FAESLDKTLEEVLVKGFNINPSAFVPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENT
                     KAGFADRNGIPVRQVLGSFVIRQDGTVHIGNTSAALLGRGGIRLSGKIDTEKDILDLN
                     IGINSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGIGTARTDGSLAIASDPAN
                     GQRKLVLDTVNIAAGQGSLTAQGYLELFKDRLLKLDIRSRAFDPSRIDPQLPAGNING
                     SINLAGELAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIIKT
                     DGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVRGHLSGDLDGGIRTFETDLSGAARN
                     LHIGKAADIRSLDFTLKGSPDTSRPIRADIKGSRLSLSGGAAVVDTADLMLDGTGVQH
                     RIRTHAAMTLDGKPFKFDLDASGGINRELTRWKGSIGILDIGGAFNLKLQNRMTLEAG
                     AERVAASAANWQAMGGSLNLQHFSWDKKTGISAKGGAHGLHIAELHNFFKPPFEHNLV
                     LNGDWDVAYGRNARGYLNISRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGA
                     RFGRINADLDIGNAFGGNMANAPLGGRITASLPDLGALKPFLPAAAQNITGSLNAAAQ
                     IGGRVGSPSVNAAVNGSSNYGKINGNITVGQSNSFSTSPLGGKLNLTVADAEVFRNFL
                     PVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRK
                     WVIDSLKFRHEGTAELSGTVGMENSGPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRY
                     SPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVVVLGEVKKEAAAPLPVNMNLTLDLN
                     DGIRFAGYGADVTIGGKLTLTAQSGGSVRGVGTVRVIKGRYKAYGQDLDITKGTVSFV
                     GPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRAGS
                     GSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTSKRSRNAQTGELNPAEQVLTVGKQ
                     LTGKLYIGYEYSISSAEQSVKLIYRLTRAIQAVARIGSRSSGGELTYTIRFDRFSGSD
                     KKDSAGNSKGK"
BASE COUNT         1552 a         2107 c         1721 g         1185 t
ORIGIN      
        1 ttgcgttgat ttgccctatg cagtgccgga caggctttgc tttatcattc ggcgcaacgg
       61 tttaatttat tgaacgaaaa taaatttatt taatcctgcc tattttccgg cactattccg
      121 aaacgcagcc tgttttccat atgcggattg gaaacaaaat accttaaaac aagcagatac
      181 atttccggag ggccgcaacc tccgaaatac cggcggcagt atgccgtctg aagcgtcccg
      241 ccccgtccga acaacacaaa aacggccgtt cgaaaccctg tccgaatagt gttagaatcg
      301 aaatctgcca caccgagtgc acgacacccg taccatgatg attaaaccga ccgccctgct
      361 cctgccggct ttatttttct ttccgcacgc atacgcgcct gccgccgacc tttccgaaaa
      421 caaggcggcg ggtttcgcat tgttcaaaaa caaaagcccc gacaccgaat cagttaaatt
      481 aaaacccaaa ttccccgtcc gcatcgacac gcaggatagt gaaatcaaag atatggtcga
      541 agaacacctg ccgctcatca cgcagcagca ggaagaagta ttggacaagg aacagacggg
      601 cttcctcgcc gaagaagcac cggacaacgt taaaacaatg ctccgcagca aaggctattt
      661 cagcagcaaa gtcagcctga cggaaaaaga cggagcttat acggtacaca tcacaccggg
      721 cccgcgcacc aaaatcgcca acgtcggcgt cgccatcctc ggcgacatcc tttcagacgg
      781 caacctcgcc gaatactacc gcaacgcgct ggaaaactgg cagcagccgg taggcagtga
      841 tttcgatcag gacagttggg aaaacagcaa aacttccgtc ctcggcgcgg taacgcgcaa
      901 aggctacccg cttgccaagc tcggcaacac ccgggcggcc gtcaaccccg ataccgccac
      961 cgtcgatttg aacgtcgtcg tggacagcgg ccgccccatc gccttcggcg actttgaaat
     1021 caccggcaca cagcgttacc ccgaacaaat cgtctccggc ctggcgcgct tccaaccggg
     1081 cacgccctac gacctcgacc tgctgctcga cttccaacag gcactcgaac aaaacgggca
     1141 ttattccggc gcgtccgtac aagccgactt cgaccgtctc caaggcgacc gcgtccccgt
     1201 caaagtcagc gtaaccgagg tcaaacgcca caagctcgaa accggcatcc gcctcgattc
     1261 ggaatacggt ttgggcggca aaatcgccta cgactattac aacctcttca acaaaggcta
     1321 tatcggctcg gtcgtctggg atatggacaa atacgaaacc acgcttgccg ccggcatcag
     1381 ccagccgcgc aactatcggg gcaactactg gacaagcaac gtttcctaca accgttcgac
     1441 cacccaaaac ctcgaaaaac gcgccttctc cggcggcatc tggtatgtgc gcgaccgcgc
     1501 gggcatcgat gccaggctgg gggcagagtt tctcgcagaa ggccggaaaa tccccggctc
     1561 ggatatcgat ttgggcaaca gccacgccac gatgctgacc gcctcttgga aacgccagct
     1621 gctcaacaac gtgctgcatc ccgaaaacgg ccattacctc gacggcaaaa tcggtacgac
     1681 tttgggcaca ttcctgtcct ccaccgcgct gatccgcacc tctgcccgcg caggttattt
     1741 cttcacgccc gaaaacaaga aactcggcac gttcatcata cgcggacaag cgggttacac
     1801 ccttgcccgc gacaatgccg atgtcccctc ggggctgatg ttccgcagcg gtggcgcgtc
     1861 ttccgtgcgc ggttacgaac tcgacagcat cggacttgcc ggcccgaacg gatcggtcct
     1921 gcccgaacgc gccctcttgg tgggcagcct ggaataccaa ctgccgttta cgcgcaccct
     1981 ttccggcgcg gtgttccacg atatgggcga cgccgccgcc aatttcaaac gtatgaagct
     2041 gaaacacggt tcgggactgg gcgtgcgctg gttcagcccg ctcgcgccgt tttccttcga
     2101 catcgcctac gggcacagcg ataagaaaat ccgctggcac atcagcttgg gaacgcgctt
     2161 ctaaaccgat acggccgctt cagacggcat tgcagcaaac tattttgaaa cagacattat
     2221 gaccgatacc gcaccgacag ataccgatcc gaccgaaaac ggcacgcgca aaatgccgtc
     2281 tgaacaccgc cccaccccgc cggcaaaaaa acgccgcccg ctgctgaagc tgtcggcggc
     2341 actgctgtct gtcctgattt tggcagtatg tttcctcggc tggctcgccg gtacggaagc
     2401 aggtttgcgc ttcgggctgt accaaatccc gtcttggttc ggcgtaaaca tttcctccca
     2461 aaatctcaaa ggcacgctgc tcgacggctt cgacggcgac aactggtcga tagaaaccga
     2521 gggggcagac cttaaaatca gccgcttccg cttcgcgtgg aaaccgtccg aactgatgcg
     2581 ccgcagcctg cacatcaccg aaatttccgc cggcgacatc gccatcgtta ccaaaccgac
     2641 tccgcctaaa gaagaacgcc ctccgctcag ccttcccgac agcatagacc tgcctgccgc
     2701 cgtctatctc gacctcttca agacgggcaa aatcagcgtc ggcaaagcct ttgacaaaca
     2761 aaccgtctat ctcgaacggc tggatgcttc ataccgttac gaccgcaaag gacaccgcct
     2821 tgacctgaag gccgccgaca cgccgtggag cagttcgtcg ggggcggcct cggtcggctt
     2881 gaaaaaaccg tttgccctcg ataccgccat ttacaccaaa ggcggactcg aaggcaaaac
     2941 catacacagt acggctcggc tgagcggcag cctgaaggat gtgcgcgccg aactggcgat
     3001 cgacggcggc aatatccgcc tctcgggaaa atccgtcatc cacccgtttg ccgaatcatt
     3061 ggataaaaca ttggaagaag tactggtcaa agggttcaac atcaatccgt ccgccttcgt
     3121 gccttccctg cccgatgccg ggctgaattt cgacctgacc gccatcccgt cgttttcaga
     3181 cggcatcgcg ctggaaggct cgctcgattt ggaaaacact aaagccggtt ttgccgaccg
     3241 caacggcatc cccgtccgtc aggttttagg cagctttgtc atccggcagg acggcacggt
     3301 gcatatcggc aatacgtccg ccgccctgct cggacggggc ggcatcaggc tgtcgggcaa
     3361 aatcgacacc gaaaaagaca tcctcgattt aaatataggc atcaactccg tcggcgcgga
     3421 agacgtactg caaaccgcgt tcaaaggcag gttggacggc agcatcggca tcggtggcac
     3481 gaccgcctcg cccaaaatct cttggcaact cggcatcggc acggcgcgca cggacggcag
     3541 cctcgccatt gcaagcgacc ccgcaaacgg acagcggaaa ctggtgctcg acaccgtcaa
     3601 catcgccgcc gggcaaggca gcctgaccgc gcaaggctat ctcgagctgt ttaaagaccg
     3661 cctgctcaag ctggacatcc gttcccgcgc attcgaccct tcgcgcatcg atccgcaact
     3721 tccggcaggc aatatcaacg gctcaataaa ccttgccggc gaactggcaa aagagaaatt
     3781 cacaggcaaa atgcggtttt tacccggcac gttcaacggc gtaccgattg ccggcagtgc
     3841 cgacattgtt tacgagtccc gccaccttcc gcgtgccgcc gtcgatttgc ggctggggcg
     3901 gaacatcatc aaaacagacg gcggcttcgg caaaaaaggc gaccggctta acctcaatat
     3961 caccgcaccc gatttatccc gtttcggttt cggactcgcg gggtctttaa atgtacgcgg
     4021 acacctttcc ggcgatttgg acggcggcat ccgaaccttt gaaaccgacc tttccggcgc
     4081 ggcgcgcaac ctgcacatcg gcaaggcggc agacatccgt tcgctcgatt tcacgctcaa
     4141 aggttcgccc gacacaagcc gcccgatacg cgccgacatc aaaggcagcc gcctttcgct
     4201 gtcgggcgga gcggcggttg tcgataccgc cgacctgatg ctggacggca cgggcgtgca
     4261 gcaccgcatc cgcacacacg ccgccatgac gctggatggc aaaccgttca aattcgattt
     4321 ggacgcttca ggcggcatca acagggaact tacccgatgg aaaggcagca tcggcatcct
     4381 cgacatcggc ggcgcattca acctcaagct gcaaaaccgt atgacgctcg aagccggtgc
     4441 ggaacgcgtg gcggcaagtg cggcaaattg gcaggcaatg ggcggcagcc tcaacctgca
     4501 acacttttct tgggataaaa aaaccggcat atcggcaaaa ggcggcgcac acggtctgca
     4561 tatcgccgag ttgcacaatt tcttcaaacc gcccttcgaa cacaatctgg ttttaaacgg
     4621 cgactgggat gtcgcctacg ggcgcaacgc gcgcggctac ctcaatatca gccggcaaag
     4681 cggcgatgcc gtattgcccg gcgggcaggc tttgggtttg aacgcatttt ccctgaaaac
     4741 gcgctttcaa aacgaccgta tcggaatcct gcttgacggc ggcgcgcgtt tcgggcggat
     4801 taacgccgat ttggacatcg gcaacgcctt cggcggcaat atggcaaatg caccgctcgg
     4861 cggcaggatt accgcctccc ttcccgactt gggcgcattg aagccctttc tgcccgccgc
     4921 cgcgcaaaac attaccggca gcctgaatgc cgccgcgcaa atcggcggac gggtcggctc
     4981 tccgtccgtc aatgccgccg tcaacggcag cagcaactac gggaaaatca acggcaacat
     5041 caccgtcggg caaagtaatt ctttcagtac ctcgcccctg ggcgggaaac tcaacctgac
     5101 cgttgccgat gccgaagtat tccgcaactt cctgcccgtc ggacaaaccg tcaaaggcag
     5161 cctgaatgcc gccgtaaccc tcggcggcag catcgccgac ccgcacttgg gcggcagcat
     5221 caacggcgac aagctctatt accgcaacca aacccaaggc atcatcttgg acaacggttc
     5281 gctgcgttcg catatcgcag gcaggaaatg ggtaatcgac agcctgaaat tccggcacga
     5341 agggacggcg gaactctccg gtacggtcgg tatggaaaac agcggacccg atgtcgatat
     5401 cggcgcggtg ttcgacaaat accgcatcct gtcccgcccc aaccgccgcc tgacggtttc
     5461 cggcaacacc cgcctgcgct attcgccgca aaaaggcata tccgttaccg ggatgattaa
     5521 aacggatcag gggctgttcg gttcgcaaaa atcctcgatg ccgtccgtcg gcgacgatgt
     5581 cgtcgtatta ggcgaagtca aaaaagaggc ggcggcaccg ctccccgtca atatgaacct
     5641 gactttagac ctcaatgacg gcatccgctt cgccggctac ggcgcggacg ttaccatagg
     5701 cggcaaactg accctgaccg cccaatcggg cggaagcgtg cggggcgtgg gcacggtccg
     5761 cgtcatcaaa gggcgttata aggcatacgg gcaggatttg gacattacca aaggcacggt
     5821 ctcctttgtc ggcccgctca acgaccccaa cctcaacatc cgcgccgaac gccgcctttc
     5881 ccccgtcggt gcgggcgtgg aaatattggg cagcctcaac agtccgcgca ttacgctgac
     5941 ggcaaacgaa ccgatgagtg aaaaagacaa gctctcctgg ctcatcctca accgcgccgg
     6001 cagtggcagc agcggcgaca atgccgccct gtccgcagcc gccggcgcgc tgcttgccgg
     6061 gcaaatcaac gaccgcatcg ggctggtgga tgatttgggc tttaccagca agcgcagccg
     6121 caacgcgcaa accggcgaac tcaaccccgc cgaacaggtg ctgaccgtcg gcaaacaact
     6181 gaccggcaaa ctctacatcg gctacgaata cagcatctcc agcgcggaac agtccgtcaa
     6241 actgatttac cggctgaccc gcgccataca ggcggttgcc cgtatcggca gccgttcgtc
     6301 gggcggcgag ctgacataca ccatacgttt cgaccgcttc tccggttcgg acaaaaaaga
     6361 ctccgccgga aacagcaaag gaaaataagc ggttttcaga cggcgcgccg ccaaaccgga
     6421 catttgaaaa cctgcttttc caccgtccgc cgccgccgtc cgcctgcaag ggaacagaat
     6481 cgatatagtg aattaacaaa aatcaggata aggcgacgaa gccgcagaca gtacaaatag
     6541 tacggaaccg attcactcgg tgctt
//
LOCUS       DAXHSF010000116         6508 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.116, whole genome shotgun sequence.
ACCESSION   DAXHSF010000116 DAXHSF010000000
VERSION     DAXHSF010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6508
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.116"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(175..2403)
                     /locus_tag="ACFM8S_001553"
     CDS             complement(175..2403)
                     /locus_tag="ACFM8S_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0030256 - type I protein secretion system
                     complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HFC6314610.1"
                     /translation="MRSAVLFIYFFLLNIFLFQPAVIHIQSNYILFMSIVSAPLPALS
                     ALIILAHYHGIAANPADIQHEFCTSAQSDLNETQWLLAAKSLGLKAKVVRQPIKRLAM
                     ATLPALVWCDDGNHFILAKTDGEGEHAQFLIQDLVTNKSAVLSFAEFSNRYSGKLILV
                     ASRASVLGSLAKFDFTWFIPAVIKYRRLFFEVLVVSVVLQLFALITPLFFQVVMDKVL
                     VHRGFSTLDVVSVALLVVSLFEIVLGGLRTYLFAHTTSRIDVELGARLFRHLLSLPLS
                     YFEHRRVGDTVARVRELEQIRNFLTGQALTSVLDLAFSFIFLAVMWYYSSTLTWVVLA
                     SLPAYAFWSAFISPILRTRLNDKFARNADNQSFLVESITAVGTVKAMAVEPQMTQRWD
                     NQLAAYVASGFRVTKLAVVGQQGVQLIQKLVTVATLWIGARLVIEGKLTVGQLIAFNM
                     LSGQVAAPVIRLAQLWQDFQQVGISVARLGDILNAPTENASSHLALPDIWGEITFEHV
                     DFRYKADGRLILQDLNLRIRAGEVLGIVGRSGSGKSTLTKLVQRLYVPEQGRVLVDGN
                     DLALAAPAWLRRQVGVVLQENVLLNRSIRDNIALTDTGMPLERIIEAAKLAGAHEFIM
                     ELPEGYGTVVGEQGAGLSGGQRQRIAIARALITNPRILIFDEATSALDYESERAIMQN
                     MQAICANRTVLIIAHRLSTVKTAHRIIAMDKGRIVEAGTQQELLAKPNGYYRYLYDLQ
                     NG"
     gene            complement(3072..3755)
                     /locus_tag="ACFM8S_001554"
                     /pseudo
     CDS             complement(3072..3755)
                     /locus_tag="ACFM8S_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            4092..4526
                     /locus_tag="ACFM8S_001555"
     CDS             4092..4526
                     /locus_tag="ACFM8S_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314611.1"
                     /translation="MDNKKEFINNLTNRYMWIYPLVLNILFLPFYQSYQSFFIALGCL
                     FALVRKMQSLDFKLQNHIVLLNIKSAWADKKVFLIRIVVSWLAVMEIWMCFISESSTW
                     VCGAFCLNSEILEKIFRGFGYSGSLYFLFILMIDLNKLRESI"
     gene            4802..5521
                     /locus_tag="ACFM8S_001556"
     CDS             4802..5521
                     /locus_tag="ACFM8S_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314612.1"
                     /translation="MKNKNYLLVFIVLHIALIVINIVFGYFVFLFDFFAFLFFANVFL
                     AVNLLFLEKNIKNKLLFLLPISIIIWMVIHISMINIKFYKFEHQIKEQNISSITGVIK
                     PHDSYNYVYDSNGYAKLKDNHRYGRVIRETPYIDVVASDVKNKSIRLSLVCGIHSYAP
                     CANFIKFAKKPVKIYFYNQPQGDFIDNVIFEINDGNKSLYLLDKYKTFFLIENSVCIV
                     LIILYLKFNLLLYRTYFNELE"
     gene            5739..>6508
                     /locus_tag="ACFM8S_001557"
     CDS             5739..>6508
                     /locus_tag="ACFM8S_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HFC6314613.1"
                     /translation="MIASRLSGNISSAIQEHKDGKVNINRFQNILADLYSLGGLGSTL
                     IEKNGNMQSWGIPLAIAGDIIAATAIATGDTGTISTEEFYNFDNWKGFGYELFEDWSR
                     WVYDWLPDGWNLWKELDRNRSGQYHIYDPLALDLDGDGIETVAAKGFSGSLFDHNGNG
                     IRTATGWVAADDGLLVRDLNGNGIIDNGAELFGDNTKLADGSFAKHGYAALAELDSNG
                     DNIINAADAAFQSLRVWQDLNQDGISQANELRTLEELG"
BASE COUNT         1975 a         1413 c         1361 g         1759 t
ORIGIN      
        1 tttgcaaagg tctctagatg agtgaaaaag aagtgcaggc tgcctaaaaa gacagaaaaa
       61 gtctttcagg cagcctgcac tttggtttca tttcagtcag taaacccagt aaacgacggt
      121 ctgaaaacgc agaacgttac gaaaaaagca gcctacacgc ccatcccccg ccttctaccc
      181 gttctgtaaa tcatacagat agcggtaata tccgttcggc ttcgccagca attcctgctg
      241 tgttcccgct tccacaatcc tgcctttatc catggcaatg atccggtgtg ccgttttaac
      301 ggtggacaga cggtgggcga taatcagcac cgtccggttg gcgcaaatgg cctgcatgtt
      361 ctgcataatc gctcgttcac tttcataatc cagcgcgctg gtggcttcgt caaaaatcag
      421 aatgcgcgga ttggtgatta acgcgcgggc aatcgcaata cgctgccgct gtccgcccga
      481 caagccggcc ccttgttcgc ccaccacggt gccgtagcct tccggcagct ccataataaa
      541 ctcgtgtgcg cccgccagtt tggctgcttc gataatgcgt tccagcggca tacccgtatc
      601 cgtcagcgcg atattgtcgc gtatgctgcg gttgagcagc acattctcct gcaagaccac
      661 gccgacctgc cgccgcagcc aggcaggagc ggccaaagcc aaatcgttgc cgtccaccaa
      721 cacccgtccc tgctccggta catacagacg ctgcaccaat ttggtgagtg tggatttgcc
      781 cgaccccgaa cgtcccacaa tccccagcac ttcccccgcc cgaatccgca ggttcaaatc
      841 ctgcaaaatc agcctgccgt ccgccttata gcggaaatcg acatgttcga acgtaatctc
      901 cccccagata tcgggcaaag ccaaatgcga agacgcattc tcggtcggcg cattcagaat
      961 atcccccaaa cgcgccaccg aaatccccac ctgctggaaa tcctgccaca actgcgccaa
     1021 acggataaca ggcgccgcca cctgtcccga gagcatatta aacgcaatca gctgccccac
     1081 cgtcagcttg ccctcaatta ccagccgtgc gccaatccac aacgtcgcca ccgtcaccag
     1141 cttctgaatc agctgcaccc cctgctggcc gaccaccgcc aacttcgtta cccgaaatcc
     1201 cgaagccaca taagccgcca actgattgtc ccaacgctgc gtcatctgcg gctccaccgc
     1261 catcgccttt accgtaccca ccgcagtgat gctttctact aaaaacgact ggttgtctgc
     1321 attgcgcgcg aacttatcgt tcagacgcgt ccgcagtatc ggactgataa atgccgacca
     1381 aaacgcatag gcaggcaacg aagccaatac cacccaagtc agagtggagc tgtaatacca
     1441 catcaccgcc agaaagataa acgaaaacgc caaatccaac accgaagtca gcgcctgacc
     1501 ggtcaagaaa ttgcgaatct gctccaattc ccgcacccga gccaccgtat cacccactcg
     1561 tctgtgctcg aaataggata aaggcaggga aagcagatgc cggaacaaac gcgcgcccaa
     1621 ttccacatca atacgtgaag tcgtatgtgc aaacagatac gtccgcaaac cgcccaacac
     1681 aatctcaaac agcgacacca ccaacaaagc caccgacacc acatccaaag tagagaatcc
     1741 ccgatgtacc agcaccttgt ccatcaccac ttggaaaaac agaggcgtaa tcagcgcaaa
     1801 cagctgcaac accaccgaca ccaccaatac ttcaaaaaac aaccggcggt atttgattac
     1861 cgccggaata aaccaggtaa agtcaaactt tgccaaactg cccaataccg aagcgcggga
     1921 agcaaccaat atcagtttgc ccgaatatct gttagaaaat tcggcaaaag acaataccgc
     1981 agacttattc gtaaccaaat cctgtatcaa aaattgggca tgctcaccct caccgtctgt
     2041 tttggccaaa ataaaatggt tgccgtcatc acaccatacc aatgcgggta aagtcgccat
     2101 agccaaacgt ttaataggct ggcggactac ctttgccttc aatcccaaag atttggcggc
     2161 caaaagccat tgcgtttcat ttaaatcgct ctgtgcggaa gtacaaaatt catgctgtat
     2221 atcggcagga ttggcggcaa tgccgtggta atgggcgagg atgatgaggg cggaaagggc
     2281 ggggagcggt gcggatacga tagacataaa taaaatatag ttagattgga tgtggataac
     2341 ggctggctgg aaaaggaata tattaagtag aaagaaatat ataaataaaa cagcagaacg
     2401 cattgtaagg atatatatgg gaattgtaaa gagaaagtat ggaaaagttc tcgtttcagg
     2461 aaggtaaaac ggcttaggaa tcgagttaga tgaggatgcc tcgcacctct cgtgcctcct
     2521 gcataccgtt aaggcacagg gttaaggtgc aggctgctcc gaactctgtt gcggtcgggt
     2581 aatgttattt tttgtgtttc aggcagcctg aaatatctgt atatttttgt tttaaataga
     2641 ttttaaagat tgataactgt tcttgacgat ttttcaagaa aggagtaaat ttcaagaaag
     2701 gagtaaagtg acttattatc aatgacaagc aacgcgcgaa gtgacaagga aaactatcta
     2761 cttaaattct aaggaggctt cgaatatcat aaaccaatca gaaacataga gataaaaatt
     2821 atgtacaaat ataatcctct tatacaattt attgcacagt tgattatgtc ttatggagca
     2881 agcgtagggt gggcacttgc tgccccacgc gtttcatatt tcaaggcagc ctgaaaccgt
     2941 gtgggcataa atgcctaccc tacatcccaa aaaacaagcg cagcctgcgt gtgtagggtg
     3001 cgaactttcg gcaggtagac acgcagtttt atattttcaa gctgagggat gcttaagaaa
     3061 agtacaaaac attaaaaaat aaggggctgt actagattag ccctaaatcc acaccaatcc
     3121 cgcaagattt ttagctgtcg ggacggtgtg ccgaagttaa atcgaaattc gcattctttc
     3181 aagaacagcg ggaaagattt gcgatcaatt ccgttctatt tgcgcaagac gcgttttgcc
     3241 tgattccaaa agttctcaat gccgtttatg tggttctgac ggtctgcaaa ttccttggaa
     3301 tggttgatgc ggtaatggat aaaaccgctt acgtccaact tgtcgtaact gctcaggctg
     3361 tcggtataaa caatgctgtc cggcatgatt ttctgtttga taacaggcat taaagtatcg
     3421 gacttggcat tatctacgac aacggtatag acccgtccgt tacgtttcag aatgccgaag
     3481 acaaccactt ttcctgccgc accgcgacca cgtttgcctt tacgccgtcc gccgaaatag
     3541 ctttcgtcca actcgacaga gccctcgaaa acctcattgg cagccaaggc cagataatgg
     3601 ctgatgacca tacggatttt gcggtagaac aggactgccg aattgggatg gatacccaaa
     3661 atatcggcag cagaacgggc ggtaacttcg agtacaaaaa aacggagcag ctctttctgt
     3721 acttttttct ttaatttgca gtgtgttatc ttcatatttc gggggtaaca tatctgctaa
     3781 tctagtacag cctcaaacaa aaaagagaaa ttttaatttc gctaaatcgc ataaagatta
     3841 atcaagagta tcattaaatg atatgagtga gcatctataa tgccaagaag agttgttaag
     3901 acataacgat tattgaaata gattgtaaaa tagatactta gatagtctga aaaacggatt
     3961 tgtgaaactt tttattacgc gccatcattt gaaaatgaaa cttaaaaaac acttatcata
     4021 ataaatattt tctttacgtt gtttgctaat aaactcagtg caatatcagc gcaatatttt
     4081 atggaaattt tatggataac aaaaaagaat ttattaataa tttaacaaat aggtatatgt
     4141 ggatctatcc attggtctta aatattctat ttctaccttt ttaccagtcc taccaatctt
     4201 tttttattgc gcttggttgt ttgtttgcac tggttagaaa aatgcaaagc ttagatttta
     4261 aattacaaaa tcatattgta ttgttaaata taaaaagtgc ttgggcagat aaaaaagtat
     4321 ttttgattag gatagtagtg tcatggttgg cagtaatgga aatatggatg tgttttattt
     4381 cggaatcatc aacgtgggta tgcggtgctt tttgtttaaa tagtgaaata ttggaaaaaa
     4441 tttttcgtgg ctttggttat tctggtagtt tatatttttt atttatattg atgattgatc
     4501 tcaataagtt aagagagagt atttgaattt cattttttgt ttgacttaaa ctcaaggaga
     4561 gtaacaatga ttggtagtgg tgatactaaa caatgcaaaa aattttctgc gtgtgatgga
     4621 aaataccacg tctacgatcc cctcgcccta gacttggacg gcgacggcat agaaacagtc
     4681 accgccaaag gcttttcagg cagcctgaag actgagagag tgaatacgat gagtatacac
     4741 tctatgccac taaattgata ttcactaaat cataccagct atattttatt taatgagaca
     4801 tatgaaaaat aaaaattatt tactagtatt tatagtttta catatagcct tgatagtaat
     4861 taatatagtg tttggttatt ttgtttttct atttgatttt tttgcgtttt tgttttttgc
     4921 aaacgtcttt cttgctgtaa atttattatt tttagaaaaa aacataaaaa acaaattatt
     4981 gtttttattg ccgatttcta ttattatatg gatggtaatt catattagta tgataaatat
     5041 aaaattttat aaatttgagc atcaaataaa ggaacaaaat atatcctcga ttactggggt
     5101 gataaaacca catgatagtt ataattatgt ttatgactca aatggatatg ctaaattaaa
     5161 agataatcat agatatggta gggtaattag agaaacacct tatattgatg tagttgcatc
     5221 tgatgttaaa aataaatcca taagattaag cttggtttgt ggtattcatt catatgctcc
     5281 atgtgccaat tttataaaat ttgcaaaaaa acctgttaaa atttattttt ataatcaacc
     5341 tcaaggagat tttatagata atgtaatatt tgaaattaat gatggaaaca aaagtttgta
     5401 cttgttagat aagtataaaa cattttttct tattgaaaac agtgtttgta tcgtattaat
     5461 tattttatat ttaaaattta atttgctttt atataggact tacttcaatg agttggaata
     5521 gttttggtaa ttttatgagc gcacgctcat ccgcgttagc agaatttgga aatatggttg
     5581 ctaatttagt ttctgcaaaa aatgagaaag atatctcgaa acgtaatgaa tattacaaac
     5641 aagctggtta tagtgcatta ttagcatttg gtaatttggc tagtaatatt gcaccaggta
     5701 gtacgtcatc gcatattgta aacggaacaa atgcctctgt gattgcaagc cgtctctctg
     5761 gaaatatatc ttcagctatt caggagcata aagatggtaa agttaatatc aaccgttttc
     5821 aaaatatttt agcggattta tattcattgg gagggttagg aagtacatta atagagaaga
     5881 atggaaatat gcagagttgg gggattccat tagcaattgc tggagatata attgcagcaa
     5941 cggctattgc cacaggagat actggtacga tatctacaga ggaattttat aattttgaca
     6001 actggaaagg ttttgggtat gagctatttg aagactggtc tcgttgggta tacgactggc
     6061 tgcccgacgg ctggaatctg tggaaagaat tggacagaaa ccgttcaggc caataccaca
     6121 tctacgaccc cctcgcccta gacctagacg gcgacggcat agaaacagtc gccgccaaag
     6181 gcttttcagg cagcctcttc gaccataacg gcaacggcat ccgcaccgcc actggctggg
     6241 ttgctgccga tgacggttta ctcgtccgcg atttgaacgg caacggcatc atcgacaacg
     6301 gcgcggaact cttcggcgac aacaccaaac tggcagacgg ttcttttgcc aaacacggct
     6361 acgcagcttt ggccgaattg gattcaaacg gcgacaacat catcaacgcg gcagacgccg
     6421 cattccaatc cctgcgtgta tggcaggatc tcaaccagga cggcatttcc caagctaatg
     6481 aattgcgtac ccttgaagaa ttgggtat
//
LOCUS       DAXHSF010000117         6477 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.117, whole genome shotgun sequence.
ACCESSION   DAXHSF010000117 DAXHSF010000000
VERSION     DAXHSF010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6477
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.117"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(58..1092)
                     /gene="purM"
                     /locus_tag="ACFM8S_001558"
     CDS             complement(58..1092)
                     /gene="purM"
                     /locus_tag="ACFM8S_001558"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFC6314614.1"
                     /translation="MSTSLSYRDAGVDIDAGDQLVENIKPFAKRTMRPEVLGDLGGFG
                     ALVEIGKKYKNPVLVSGTDGVGTKLKLAFDWDKHDTVGIDLVAMSVNDILVQGAEPLF
                     FLDYFACGKLDVPRATDVIKGIAQGCEESGCALIGGETAEMPGMYPVGEYDLAGFAVG
                     VVEKENVITGRSIGAGDVVLGLASNGAHSNGYSLIRKIIERDNPDLDAEFDNGKTLRE
                     AVIAPTRLYVKPILAALEKFTIKGMAHITGGGITENVPRVLPENTVAQIDAKSWELPK
                     LFQWLQKAGNVETQEMYRTFNCGIGMVVIVAAEDADAVQGLLGEQGETVYRLGLIRER
                     QGDEHQTQVA"
     gene            complement(1612..1696)
                     /locus_tag="ACFM8S_001559"
     tRNA            complement(1612..1696)
                     /locus_tag="ACFM8S_001559"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1660..1662),aa:Leu,seq:tag)
     gene            1850..2527
                     /locus_tag="ACFM8S_001560"
     CDS             1850..2527
                     /locus_tag="ACFM8S_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314615.1"
                     /translation="MPAALIKDFLLTQGLKLPLDEVRAAYLTAQTVMDMGTASIDRSV
                     LWRSDEDWKLADYLSCDNVREDALKRLFMALDSVFSRSTGVRSAAVYALMPSENQAFQ
                     LICLSRQGEVLENLWDLDEAAGKVSLACRSAQSGWMNVASDVRRWLDLGELSGERNHA
                     SAAQISIPVCTESGGVLGVVHVEFECAECAGTAAQVEWVALALALSEPLKLLLGMSAG
                     KDGSEDV"
     gene            2520..3113
                     /gene="ribA"
                     /locus_tag="ACFM8S_001561"
     CDS             2520..3113
                     /gene="ribA"
                     /locus_tag="ACFM8S_001561"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFC6314616.1"
                     /translation="MSEMLNHVASCRLPTEWGVFTMHGFEEANGQEHVALTVGNFSDG
                     NPVLTRIHSECLTGDALFSRKCDCGPQLEAAMRAVQTEGRGIIVYLRQEGRGIGLINK
                     IRAYHLQDQGMDTVEANLALGLPVDARDFRLAQSIYEYLGIRSVKLLTNNPEKIQTLK
                     DAGINVVERIPLHVGENLENKRYLQTKADKLGHLMSE"
     gene            3269..4282
                     /locus_tag="ACFM8S_001562"
     CDS             3269..4282
                     /locus_tag="ACFM8S_001562"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC6314617.1"
                     /translation="MIFSIIVPIYNVEKYLRCCVDSVLAENFADYEMILVDDGSPDGC
                     GKICDEYAGKYPHIKVIHQENGGLSDARNAGIRAAKGDYLIFLDSDDYWADTNCSKNA
                     RGGILFDLQQLADKKVDLILHPSSLNYHVIPKGADFSDNDFVRHFETLVEGRYYIANA
                     WTKIVRREIIIKNNLFFPKGYIHEDFPYSLKLARFVKTFAFYDNPFYQYRVLGNSISH
                     NVKYKNFSDMLAHLDRGVDFLVENRNSPIYGGLQKFVFDNIGYLRSISVRLYFSKNII
                     VVYREYFSFKEKCRKIFGAKAIRPVFIGKTAFIIGLPILRLLVPPMLYPAIKAVYQKF
                     FSE"
     gene            4497..5834
                     /gene="ribBA"
                     /locus_tag="ACFM8S_001563"
     CDS             4497..5834
                     /gene="ribBA"
                     /locus_tag="ACFM8S_001563"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HFC6314618.1"
                     /translation="MTDTAGLRRRNLRQWIAEHYGGLQTRFAEAVALNTGELSALLKN
                     KSFGEKKARKIEQAAKMPAFWLDTEHTARPPEHTGKHTMSHISPIPEILADIKAGKMV
                     IITDAEDRENEGDLLMAAQFVTPEAINFMIKHARGLVCLPMDGEMVEKLGLPMMTQKN
                     GAQYGTNFTVSIEAAHGITTGISAADRALTIQTAVSPTAKPEDIVQPGHIFPLRAQKG
                     GVLVRAGHTEAGVDLAQMNGLIPASVICEIINDDGTMARMPELMKFAEEHKLKIGTIA
                     DLIEYRSRTESLLEDMGNAPVQTPWGEFQQHVYVDKLSGETHLALVKGTPSADTETLV
                     RVHEPFSVMDFIQANPRHSWSLPKALEHIQQAESGVVILLHRTEDGASLLDRTLPKGA
                     NQAYKWDSKSYGIGAQILAGLNVKKLRVLGQPSSFTGLTGFGLEVVGFEEAEK"
     gene            complement(5994..6110)
                     /locus_tag="ACFM8S_001564"
     CDS             complement(5994..6110)
                     /locus_tag="ACFM8S_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFC6314619.1"
                     /translation="MPSESLSDGILFGLENTYAVLSLKERIQKTKATGIYRK"
     gene            complement(6142..6477)
                     /locus_tag="ACFM8S_001565"
                     /pseudo
     CDS             complement(6142..6477)
                     /locus_tag="ACFM8S_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232504.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="N-6 DNA methylase"
BASE COUNT         1599 a         1690 c         1580 g         1608 t
ORIGIN      
        1 ctctataaaa atgccgtcca aacccatgtt tgagacggca tttcgctata gaagcaatca
       61 ggcaacctgg gtttgatgct cgtctccctg acgctcacgg atcaaaccta aacggtaaac
      121 tgtttcacct tgttcaccca agagaccctg aaccgcatcg gcatcttcgg cagcaacaat
      181 aacgaccatg ccgatgccgc agttaaaggt tcggtacatt tcttgggttt ccacattgcc
      241 cgccttttga agccattgga agagcttggg caattcccac gatttagcat cgatttgtgc
      301 aaccgtgttt tcaggcaaca cgcgcggcac gttttcggta atgccgccgc cggtaatgtg
      361 tgccatacct ttaatggtaa atttttccaa agcggcaaga atcggtttca catacagacg
      421 ggtcggcgca ataacagcct cacgcaaggt tttgccatta tcaaactcgg catccaaatc
      481 gggattgtcg cgttcgatga ttttacggat aagggaatag ccgtttgaat gtgcgccgtt
      541 ggaagccaaa cccaatacca catcgcccgc gccgatgctg cggccggtaa tgacattctc
      601 tttttccact acgccgacgg caaaacccgc caaatcgtat tctccgacgg gatacatgcc
      661 cggcatttcg gcagtttccc cgccaatcag ggcgcagccg gattcttcgc aaccttgtgc
      721 aatgcctttg atgacatcgg ttgcgcgcgg aacatccaat ttgccgcagg cgaaataatc
      781 caagaaaaac agaggttctg ctccttgaac caaaatatcg ttgacactca ttgcaacaag
      841 gtcgatgccc accgtatcgt gtttatccca atcaaaggca agtttgagct tggtacccac
      901 gccgtccgta ccgctgacca atacgggatt tttatatttc ttgccgattt cgaccaatgc
      961 gccgaagccg cccaaatccc ccaatacttc cgggcgcatc gtgcgtttgg caaaaggttt
     1021 gatgttttcg accagttggt cgcctgcgtc gatatcgaca cctgcatcgc ggtaactcaa
     1081 tgaagtactc atcatttttc cttggtaaat ggggattgga cggtaaaata acggggcgta
     1141 ttctacctta tttcgcgttt gcaagttcag atttttagac aatattgtaa acaatccgcc
     1201 atatgcccgc gcgtgtcggg tttggcggga tcgtccgcag gattaacggg cagaaacccg
     1261 cctgcccttc ccctcaattc cttatatatc gcgttccatc aaaagacgca ttgcttttct
     1321 taaccattcc ttttggcaga cgggcggaag gggttttttg atgccatcat caaaatcaat
     1381 attttcttct ttccagttga aaccccggca ttaggggcgg tgaatctgat tgcgggcgga
     1441 agcacccgct tctgattcgg ggtagaacaa atggcggcac tttatgtacc gttctgcgtg
     1501 ttgaaacata taggcagata aaaaagccgc ccgctgaaaa gcagacgact tatgttttgt
     1561 ggcactaatt tgtcccgata agcattaact atatgattta tttatcatta ttggtgcgga
     1621 cggagagact cgaactctca cacctttcgg cgccagaacc taaatctggt gcgtctacca
     1681 atttcgccac gtccgcatgg gaattggacg attatacaga ttttgttttt ttgtgcaagg
     1741 ttttcggcgg ggctgttgat ggcttggggt ttggggcggt aaaatctgtt tttcgtccgc
     1801 ctgacatcgg aatccggcgg ttttttgttt ttattgacgg aatttgggta tgcctgctgc
     1861 tttgattaag gattttctgc tgactcaggg tttgaagctg ccgcttgacg aggttcgggc
     1921 ggcgtatctg acggcgcaga cggtaatgga tatggggacg gcttcgatag accgttcggt
     1981 tttgtggcgc agtgatgagg attggaaact tgccgattac ctgtcgtgcg acaatgtccg
     2041 cgaagatgca ctgaaacggc ttttcatggc tttggattcg gtgttttcgc gctcgacagg
     2101 cgtgcggagt gcggcggtct atgccttgat gccatctgaa aaccaggctt tccaactgat
     2161 atgcctgtcc cgacagggcg aggttttgga aaacctgtgg gatttggatg aagcggcagg
     2221 caaggtttcg ctggcttgcc gttcggcgca aagcggttgg atgaatgttg cctcggatgt
     2281 acgccgttgg ttggatttgg gggagctttc gggagaacgc aatcatgctt cggcggcgca
     2341 aatttccatt ccggtctgca cggaaagtgg cggtgtgttg ggcgtggttc atgtggaatt
     2401 tgaatgcgca gagtgtgcgg gtacggcagc acaggtggaa tgggtggctc ttgccttggc
     2461 tttgtccgaa cctttgaaac tgctgttggg tatgtctgcc ggaaaagatg ggagtgaaga
     2521 tgtctgaaat gttgaaccat gtggcatcct gccgcctgcc gaccgaatgg ggcgtattta
     2581 cgatgcacgg ctttgaagag gcaaacgggc aggaacacgt cgcgctgacc gtcggtaatt
     2641 tttcagacgg caatccggtt ctgacgcgca tccactccga atgcctgacg ggcgatgcgc
     2701 tgttctcgag aaaatgcgac tgcggacctc aacttgaagc ggccatgagg gcggtacaga
     2761 cagaggggcg cggcatcatc gtctatctgc gtcaggaagg acgcggcatc gggctgatta
     2821 acaaaatccg cgcctatcat ctgcaagacc aaggtatgga tacggttgaa gccaatttgg
     2881 cactcgggct gcccgtcgat gcccgcgatt tccgtttggc gcaatctatc tacgaatatc
     2941 tgggcatccg ctcggtcaaa ctgctgacca acaaccccga aaaaatccaa accctgaaag
     3001 atgcggggat taacgtggtc gaacgcatcc ccctgcacgt cggggaaaac cttgaaaaca
     3061 aacgctacct ccaaaccaaa gcagacaagc tgggacatct gatgtcggaa taaggcaaag
     3121 ttgcagggaa cgggcatcct gcgccgcctt tcgggaaaca ggtttccata ccttgataaa
     3181 gcaataagtt ttatagtgga tgcaaacgca ttgcgagcgc gggtttgagg catacgcgca
     3241 aacatcttaa tataatggat tgatatttat gattttctcc atcatcgtcc ctatttacaa
     3301 tgtggaaaaa tacctccgct gctgcgtgga ttccgtgctt gccgaaaatt ttgccgatta
     3361 tgaaatgatt ttggtcgatg acggttcgcc ggacggctgc gggaagattt gcgacgaata
     3421 tgcaggcaaa tatccgcata taaaagtgat tcatcaagaa aacggcgggc tgtcggatgc
     3481 ccgcaacgcc ggtatccggg cggcaaaagg cgattacctg atctttttgg acagcgacga
     3541 ctattgggcc gataccaact gttcaaaaaa cgcgaggggg gggattctct ttgatttaca
     3601 acaacttgca gacaaaaagg ttgatttgat cctgcatccc tcgtccctca attaccacgt
     3661 catccccaaa ggggcggact tttcggataa tgattttgtc cgccattttg aaacgttggt
     3721 ggaggggcgg tactatatcg ccaacgcgtg gacaaaaatc gtcaggcggg aaataatcat
     3781 caaaaacaat ctgtttttcc caaaaggata cattcacgag gatttcccgt acagtttgaa
     3841 attggcgcgt tttgtcaaga cttttgcctt ttacgataac cctttttatc aataccgcgt
     3901 tctcggcaac tctatcagcc acaacgtcaa atacaaaaat ttcagcgata tgctggcgca
     3961 tctcgaccgg ggtgtggatt ttttagtcga aaacagaaat tcccccatct acggcggttt
     4021 gcaaaaattt gtcttcgaca atatcggcta tctgagatct atatcggtaa ggctttattt
     4081 ttccaaaaac attatcgttg tctaccgaga atatttttca tttaaagaga aatgcagaaa
     4141 gatattcggc gcgaaggcaa tccgtccggt ttttatcggg aagaccgcat tcatcatagg
     4201 attgccgata ttgcgcctgc tcgtaccgcc tatgctgtac ccggcaatca aggccgttta
     4261 tcaaaaattt ttttcggaat aagcttccgg caaaccccga atcggaagcg ggcgggaaga
     4321 aacagccgcc ccgccggcgg ggttgcggca atgccgtctg aagccacgaa tccggcttca
     4381 gacggcatct gtttaccaaa aagcaaataa ttcggtttgg caaaaaaaac agatttgctt
     4441 tttggtaaat acgcgcttac aatccgctac atccgatttc tacaaaggat gaaacgatga
     4501 ccgacacagc cggtctgcgc cgccgcaacc tgcggcaatg gatagcagaa cactacggcg
     4561 gtttgcaaac ccgttttgcc gaagccgttg ccctcaacac aggcgaactc tccgcccttt
     4621 tgaaaaacaa atccttcggc gagaaaaaag cccgtaaaat cgaacaggcg gcaaaaatgc
     4681 ccgccttctg gctcgatacc gaacacaccg cccgcccgcc cgaacacaca ggaaaacaca
     4741 ccatgtccca tatttccccc atccccgaaa tcctagccga catcaaagcc ggcaaaatgg
     4801 tcatcatcac cgatgccgaa gaccgagaaa acgaaggcga cctgctgatg gcggcgcaat
     4861 tcgtcacgcc cgaagccatc aacttcatga tcaaacacgc gcgcggcttg gtctgcctgc
     4921 cgatggacgg cgaaatggtc gaaaaactcg ggctgccgat gatgacccaa aaaaacggcg
     4981 cgcaatacgg caccaacttt accgtctcca tcgaagccgc acacggcatt accaccggca
     5041 tttccgccgc cgaccgcgcc ctgactattc aaaccgccgt ttccccgacc gctaaacccg
     5101 aagacatcgt ccaacccggt catatctttc cgcttcgcgc ccaaaaaggc ggcgtactcg
     5161 tccgcgccgg acacaccgaa gccggcgtcg acctggcgca aatgaacggg ctgattcctg
     5221 cctccgttat ttgcgaaatc atcaacgacg acggcacgat ggcgcgtatg cccgaactga
     5281 tgaaattcgc cgaagaacac aagctcaaaa tcggcacgat tgccgacctc atcgaatacc
     5341 gcagccgtac cgaaagcctg cttgaagaca tgggcaatgc gcctgtacaa accccgtggg
     5401 gcgagttcca acaacacgtt tacgtcgaca aactctccgg cgaaacccac ctcgccctcg
     5461 tcaaaggcac gccctccgcc gacaccgaaa ccctcgtccg cgtccacgaa cccttcagtg
     5521 tgatggactt cattcaagcc aacccgcgcc attcatggtc gctgcccaaa gcccttgagc
     5581 acatccaaca agccgaaagc ggcgtcgtca tcctcttaca ccgcaccgaa gacggcgcat
     5641 ccctgctcga ccgaacccta cccaaaggcg caaaccaagc ctacaaatgg gacagcaaaa
     5701 gctacggcat cggcgcacaa atcctcgccg gcctcaacgt caaaaaactg cgcgtcctcg
     5761 ggcagccctc atctttcacc ggcctgaccg gcttcggttt ggaagtcgtc ggctttgaag
     5821 aagcggaaaa ataatatagt aaattcaaat actttatatt tgctttattt attgcattat
     5881 ttccgtgcaa acgaaaaccc ggtctgttgg gttggatttt gttttttcaa atttcgggta
     5941 acttctaatt cgtcattccc gcgctggcgg gaatccggtt cgtcgggttt ttgtcatttc
     6001 cgataaattc ctgtggcttt ggttttttgg attctctctt tcagggaaag aacggcataa
     6061 gtattttcca aaccaaacaa aatgccgtct gaaaggcttt cagacggcat tttaagtttg
     6121 accggtttca ttcatcggta tttatgaatt gaatttcaac atcgccaatc tatccttaat
     6181 ctctttttcc aattcggcag attcggcgaa aagtttatcc aaatccgctg aaaatcccgc
     6241 cattttttgc gcaaattcgt cggcggaaat atccacataa tcaatcttta cctcgaaata
     6301 ctgccccgcc gacaagctgt gattcttcgc tttgatttca tcgtagccga ttaccacact
     6361 gaaatcttcc actgcctgtt tgtgcgtgaa ggtattgcag atttttttgt tcttcttcgc
     6421 gggaaagtac ggtttttttg ccgtctttaa ttttttcgcc caagcccgat gcgtcga
//
LOCUS       DAXHSF010000118         6365 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.118, whole genome shotgun sequence.
ACCESSION   DAXHSF010000118 DAXHSF010000000
VERSION     DAXHSF010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6365
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.118"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            171..2006
                     /gene="edd"
                     /locus_tag="ACFM8S_001566"
     CDS             171..2006
                     /gene="edd"
                     /locus_tag="ACFM8S_001566"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFC6314620.1"
                     /translation="MNTTPIHSKLAEITGRIIERSRPTREKYLAKIRSAKQMGRLERN
                     QLGCSNLAHGYAAMPKSIKIEMLQETVPNLGIITAYNDMVSAHQPFKDFPDQIKDEAQ
                     KNGATAQVAGGTPAMCDGITQGYAGMELSLFSRDVIAMSTAIGLSHQMFDGSLFMGVC
                     DKIVPGLMIGALSFGHIPGIFVPAGPMSSGIGNKEKARTRQLFAEGKVGRNELLKSEM
                     GSYHSPGTCTFYGTANSNQMMMEMMGVHLPAAAFVHPYTDLREALTRYAAGHLARGIK
                     NGTIKPLGEMLTEKSFINALIGLMATGGSTNHTMHLVAMARAAGVILNWDDFDEISSI
                     IPLLIRVYPNGKADVNHFTAAGGLPFVIRELLNAGLLHDDVDTVVGRGMRHYTKEPFL
                     IDGKLEWREAPETSGNDDILRKADNPFSPDGGLRLMKGNIGRGVIKVSAVREGCRIIE
                     APAIVFNDQREVLSAFERGELERDFVCVVRYQGPRANGMPELHKLTPPLGILQDRGFK
                     VALLTDGRMSGASGKVPASIHMTPEALMGGNIAKIRTGDLIRFDSVTGELNVLIDEAE
                     WNAREVEHIDLGANQQGCGRELFANFRSMTSSAETGAMSFGGEFA"
     gene            2187..2825
                     /locus_tag="ACFM8S_001567"
     CDS             2187..2825
                     /locus_tag="ACFM8S_001567"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016833 - oxo-acid-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFC6314621.1"
                     /translation="MSKLTPREILTAGAVVPVMAIDDLSTAVDLSHALVEGGIPTLEI
                     TLRTPVGLDAIRLIAKEVPNAIVGAGTVTNPEQLKAVEDAGAVFAISPGLHESLAKAG
                     HNSGIPLIPGVATPGEVQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTG
                     GISLATAPEYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA"
     gene            complement(3081..4122)
                     /locus_tag="ACFM8S_001568"
                     /pseudo
     CDS             complement(3081..4122)
                     /locus_tag="ACFM8S_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225132.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
     gene            4285..5325
                     /gene="mutY"
                     /locus_tag="ACFM8S_001569"
     CDS             4285..5325
                     /gene="mutY"
                     /locus_tag="ACFM8S_001569"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFC6314622.1"
                     /translation="MNTPIPFSERLIRWQKQHGRHHLPWQVKNPYCVWLSEIMLQQTQ
                     VATVLDYYPRFLEKFPTVQILAAAPQDEVLSLWAGLGYYSRARNLHKAAQQVVGQFGG
                     TFPSERKDLETLCGVGRSTAAAICAFAFNRRETILDGNVKRVLCRVFAQDGNPQDKKF
                     ENALWTLAESLLPSENADMPAYTQGLMDLGATVCKRTKPLCHQCPMADICEAKKQNRT
                     AELPRKKTAAEVPTLPLYWLIVRNRDGAILLEKRPAKGIWGGLYCVPCFESLNGLSDF
                     AAKFSLTMADMDEQTALTHRLTHRLLLITPFEAQMPSESPSDGIWIKPTHLKDYGLPK
                     PLEIYLNGNRLE"
     gene            5742..6302
                     /gene="sodC"
                     /locus_tag="ACFM8S_001570"
     CDS             5742..6302
                     /gene="sodC"
                     /locus_tag="ACFM8S_001570"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFC6314623.1"
                     /translation="MNMKTLLALAVSAVCSVGVAQAHEHNTIPKGASIEVKVQQLDPV
                     NGNKDVGTVTITESNYGLVFTPDLQGLSEGLHGFHIHENPSCEPKEKEGKLTAGLGAG
                     SHWDPKGAKQHGYPWQDDAHLGDLPALTVLHDGTATNPVLAPRLKHLDDVRGHSIMIH
                     TGGDNHSDHPAPLGGGGPRMACGVIK"
BASE COUNT         1644 a         1786 c         1526 g         1409 t
ORIGIN      
        1 aggcaacgcc gtactggttt aaagttaatc cactatagtg aggttttcag acgacccctt
       61 gtaagatttg aaaggtcgtc tgaaacgcca aattccccac acccaataca aaaaatcaga
      121 aacgcgcaaa caaaatcccc ataccaccca cttccgacag gagaccgacc gtgaacacta
      181 ctcctatcca ctccaaactc gccgaaatca ccgggcgcat tattgaacgc agccgtccga
      241 cgcgtgaaaa atatctggcg aagatccgca gtgccaaaca aatgggacgc ttagagcgca
      301 accagctcgg ctgcagcaac ttggcacacg gctatgctgc catgcctaaa agtatcaaaa
      361 tcgaaatgct tcaggaaacc gtccccaact taggcatcat caccgcctac aacgacatgg
      421 tttccgcaca ccagccgttt aaagacttcc ctgaccaaat caaagacgaa gcgcagaaaa
      481 acggcgcgac cgcccaagtc gccggcggca cgcccgccat gtgcgacggc atcacgcaag
      541 gctacgccgg catggaattg tcgctgttct cccgcgacgt gattgccatg agtaccgcca
      601 tcgggctgtc gcatcaaatg tttgacggca gcctgtttat gggcgtatgc gacaaaatcg
      661 ttccaggttt gatgataggc gcgctttcgt tcggtcatat tccgggtatc ttcgtccccg
      721 caggcccgat gtccagcggt atcggcaaca aagaaaaagc ccgcacccgc cagcttttcg
      781 ccgaaggcaa ggtcggacgc aacgaacttt tgaaaagcga aatgggttct taccacagcc
      841 cgggcacctg cactttctac ggcacggcaa actccaacca aatgatgatg gaaatgatgg
      901 gcgtgcacct gcctgccgcc gccttcgtcc acccttacac cgacctgcgt gaagcgctga
      961 cccgctacgc cgccggacac ctcgcgcgcg gcatcaaaaa cggcacgatt aaacctttgg
     1021 gcgaaatgtt gaccgaaaaa tcctttatca acgccttgat tggcctgatg gcaaccggcg
     1081 gttcgaccaa ccacaccatg cacctcgtcg ccatggcacg cgccgccggc gtgattttga
     1141 actgggacga cttcgacgaa atttcctcca tcatcccgct gctcattcgc gtttatccga
     1201 acggcaaggc cgacgtgaac cactttaccg cagcgggcgg actgcctttc gttatccgcg
     1261 aattgctgaa tgcaggcctg ttgcacgacg atgtcgatac cgtcgtcgga cgcggtatgc
     1321 gccactacac caaagagcct ttcctcatcg acggcaaact cgaatggcgc gaagcccccg
     1381 aaaccagcgg caacgacgac atcctgcgca aagccgataa cccgttctcc ccagacggcg
     1441 gtctgcgcct gatgaaaggc aacatcggtc gcggcgtgat taaagtgtcc gccgtgcgcg
     1501 aaggctgccg catcatcgaa gcacctgcca tcgtgttcaa cgaccaacgc gaagtattgt
     1561 ccgcatttga acgcggcgag ttggaacgcg atttcgtctg cgtcgtccgc taccaaggcc
     1621 cgcgcgccaa cggtatgcct gaattgcaca aactgacccc gcctctgggc atcctgcaag
     1681 accgcggctt caaagtggcg ttgctgaccg acggccgtat gtccggcgcg tccggcaaag
     1741 tgcccgcatc catccatatg acccccgaag ccctgatggg cggcaacatc gccaaaatcc
     1801 gtaccggcga cctgatccgc ttcgattccg tgaccggcga actcaacgtc ctgattgacg
     1861 aagccgaatg gaatgcccgc gaagtcgaac acatcgactt gggcgcgaac caacaaggct
     1921 gcggccgcga actcttcgcc aacttccgca gcatgaccag cagcgcggaa accggtgcca
     1981 tgagtttcgg cggcgaattt gcctgatgcg cgtttcagac ggccttttca gaccgaaggc
     2041 cgtctgaaaa attattcaag tgttttaaga tagacgtagg ttggattctc gaatccgaca
     2101 cagccgtcca agatgtcggt ttcttgaatc cgacctacga cctgtcccat cttaataaaa
     2161 taccccattc cacccggaga accgaaatgt ccaaactgac cccccgcgaa attttgaccg
     2221 ccggcgcagt tgtgccggta atggcgattg acgacttaag caccgccgtc gatttgtccc
     2281 acgcccttgt cgaaggcggc atccccaccc tcgaaatcac cctgcgcacc cctgtcggcc
     2341 tcgatgccat ccgcctgatt gccaaagaag tgcccaacgc catcgtcggc gcgggtacgg
     2401 ttaccaaccc tgaacagctc aaagccgtcg aagacgcagg cgcggttttc gccatcagcc
     2461 cggggctgca cgaatccctc gccaaagccg gccacaacag cggcatcccc ctgattcccg
     2521 gcgttgccac tccgggcgaa gtccaactgg ccttggaaca cggcatcgac accctcaaac
     2581 tcttccccgc cgaagtcgtc ggcggcaaag ccatgctcaa agccctttac ggcccttacg
     2641 ccgatgttcg cttctgccca accggcggca tcagcctggc aactgccccc gaatacctgg
     2701 cactgcccaa cgtcctgtgc gtcggcggct cttggctgac accgaaagaa gccgtgaaaa
     2761 acaaagactg ggacaccatc acccgcctcg ccaaagaagc ggcggcattg aaacccaaag
     2821 cctgaaccgc atcgtaaaaa tgccgtctga aaaacctttc ccgtttcaga cggcattttg
     2881 tcgattgagg gcacagtcgg catacacggc agcactgatc agacataccg cccctaaaat
     2941 gcccatccgc cttccgcata ataaaaataa cggtcagttc attcgacagc agccggacag
     3001 cccactacac ggctgaaaaa atgccgtctg aaacgcattc agacggcatc cgcttaaaaa
     3061 aacaactgat tcaacgccga ttaatccgct tccaaaacca ctttcatcgc ttgattttcg
     3121 gcggcgtgtt tgaacacgtc gtaggctttt tccaattcgc tgaatttgaa acggtgggtc
     3181 aacattttgg tgtaatcgac ggagctgctg gaaattgcct tcatcagcat ttcggtggta
     3241 ttggcgttta ccaggccggt agtgatggca agctttttaa tccacagttt ttccagtttg
     3301 aaatcaacgg attgaccgtg tacgccgaca acggcgatat ggccgccggg tttcacgatg
     3361 tcttggcaca tattccaagt tgcagggata ccgaccgctt cgatggcaca atctacgccg
     3421 tcttcgccga cgatggcgaa aacttgtttg gagacgtcgc cggaagcggg gctgatggta
     3481 tgggtcgcgc ccaactcttt cgccagtttc aaacggtttt cgtccatatc gcacacgata
     3541 atggcggcgg ggctgtacag ttgggcggtc aacaaggcgg acataccgac aggagcggca
     3601 cctgcgatga atacggtgtc gccgggtttg acatcgccgt attgcacgcc gatttcgtgt
     3661 gcggtcggca aagcgtcgct caacaacagg gcgacttctt cgttgacgtt gtcgggcagc
     3721 ggcacgaggc tgttgtcggc ataaggcgtg cggacgtatt ctgcctgcgt gccgtcaatc
     3781 atgtagccca aaatccagcc gccgttgcgg caatgcgaat aaagttggat tttgcagttg
     3841 tcgcaagtac agcatttgct gacgcatgaa ataatgactt tatcgccgac tttgatgttt
     3901 tttacagcct cgccgacttc ttctacaata ccgatgcctt catgaccgag aatacggccg
     3961 tcggcgactt cgggtttttt tgcctttcca aatacccaaa tcggtaccgc aaatcgtggt
     4021 tttgacgatt ttcaccaccg catcggtcgg atcgataatc tgcggacggg gtttttcttc
     4081 aaaacggatg tcgtttgcgc cgtgataaac cattgctttc atgctgatac tccttgcttg
     4141 ttgataaata atttcaatac cgcaataaag tttctttata tgagttatat gcccctacaa
     4201 aaaataagta aataagaatt attttcacaa tgttatacaa taacatactg ttttaaatat
     4261 aaataaaacc accgattgat attaatgaac acacccatcc ccttctccga acggctcatc
     4321 cgctggcaaa aacaacacgg tcgccaccac ctcccttggc aggtcaaaaa cccttattgc
     4381 gtctggcttt ccgaaatcat gctccagcaa acgcaggtcg ccaccgtgtt ggactactat
     4441 ccgcgtttct tagaaaaatt cccgactgtt caaatacttg ccgccgcgcc gcaagacgag
     4501 gttttatcct tgtgggcagg cttgggctat tacagccgcg cgcgcaatct gcacaaagcc
     4561 gcgcaacaag tcgtcggaca attcggcggc acgtttccgt cggagcgtaa agacttggaa
     4621 accctctgcg gcgtaggcag aagcaccgcc gccgccattt gcgcctttgc cttcaaccgc
     4681 cgcgagacca ttttggacgg caacgtcaaa cgcgtactct gccgtgtctt tgcccaagac
     4741 ggcaatccgc aagacaaaaa atttgaaaac gcgctctgga cacttgccga aagcctgctg
     4801 ccgtctgaaa acgccgatat gcctgcctat acacaaggtt tgatggattt gggcgcgacc
     4861 gtgtgcaaac ggacgaaacc cttgtgccac caatgcccga tggcggacat ctgcgaagcg
     4921 aaaaagcaaa accgcaccgc cgagctgccg cgcaaaaaaa ccgccgccga agtaccgacc
     4981 ctgccgcttt actggctgat tgtccgcaac cgggacggcg cgattttgct ggaaaaacgc
     5041 cccgccaaag gcatttgggg cgggctgtat tgcgtgccgt gttttgaaag tttgaacggg
     5101 ctttccgact ttgccgccaa attctccctg accatggcag atatggacga acaaaccgcc
     5161 ctgacccacc gcctgacgca ccggctgcta ttgattacgc cctttgaagc acaaatgccg
     5221 tctgaaagcc cttcagacgg catttggata aagccgacgc atttgaaaga ttacggcctg
     5281 cccaagcctt tggaaattta tttaaacggt aataggttag aataaacaaa ataaacccat
     5341 tgaactgttg tttgcaggta tcgcagcaag aacaaccgat gaatttgggt cgtattttag
     5401 gcggcgggat aatgttcaaa tgggacattt ggaacggaag aagtcggcaa tttaaaaagg
     5461 atttaaaaag caaagaaggt caaaaacatg aacacaaact taaatgacaa agacaaagcc
     5521 atggataccg caatcaggtt tcagaaaagg atgaggattc cgaaattttt ctttttaatt
     5581 ctctaaatca caatggtttt ggcatttatc caagacgtga taacgggttc taattttctg
     5641 caaataacaa ttaatgtaaa attttcgtaa aaatttatcg gcttttaaaa caaaattgac
     5701 taaaatagtc gcgagttttt actgcaataa aggagattgc aatgaatatg aaaaccttat
     5761 tagcactagc ggttagtgca gtatgttcag ttggtgttgc gcaagcacac gagcataata
     5821 cgatacctaa aggtgcttct attgaagtga aagtgcaaca acttgatcca gtaaacggta
     5881 acaaagatgt gggtacagtg actattactg aatctaacta tggtcttgtg tttacccctg
     5941 atttacaagg attaagcgaa ggcttacatg gtttccacat ccatgaaaac ccaagctgtg
     6001 agccaaaaga aaaagaaggt aaattgacag ctggtttagg cgcaggcagt cactgggatc
     6061 ctaaaggtgc aaaacaacat ggttacccat ggcaagatga tgcacactta ggtgatttac
     6121 ctgcattaac tgtattgcat gatggcacag caacaaatcc tgttttagca ccacgtctta
     6181 aacatttaga tgatgttcgc ggtcactcta ttatgatcca cacgggtggt gataatcact
     6241 ccgatcatcc agctccactt ggcggtggcg gcccacgtat ggcatgtggc gtgattaaat
     6301 aattcgattg ttcgaaacga aaagtgcggt aaattttgac cgcacttttt tgctagatat
     6361 ttagc
//
LOCUS       DAXHSF010000119         6363 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.119, whole genome shotgun sequence.
ACCESSION   DAXHSF010000119 DAXHSF010000000
VERSION     DAXHSF010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6363
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.119"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(46..312)
                     /locus_tag="ACFM8S_001571"
     CDS             complement(46..312)
                     /locus_tag="ACFM8S_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFC6314624.1"
                     /translation="MARMVFCVKLNKEAEGMKFPPLPNELGKRIFENVSQEAWAAWTR
                     HQTMLINENRLSLADPRAREYLAQQMEQYFFGDGADAVQGYVPQ"
     gene            complement(385..855)
                     /gene="rlmH"
                     /locus_tag="ACFM8S_001572"
     CDS             complement(385..855)
                     /gene="rlmH"
                     /locus_tag="ACFM8S_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFC6314625.1"
                     /translation="MNITVLAVGTKMPRWVDEAVAEYAKRFGRDVAYALKEIKPEKRG
                     AGVNAAQGMAAEEKRILEAIPQGAFLVVLDERGKAPTSVELAEHLKSWQQNGEHVCFV
                     IGGADGMTDRLKQQARMMMRLSSLTLPHGMVRVFLTEQLYRAVSILHNHPYHRE"
     gene            complement(909..1295)
                     /gene="rsfS"
                     /locus_tag="ACFM8S_001573"
     CDS             complement(909..1295)
                     /gene="rsfS"
                     /locus_tag="ACFM8S_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFC6314626.1"
                     /translation="MNEQELQDLQKMVGVAVNALEDIKAKDISVLETQDKTSLFARMI
                     IASGDSTRQVKALANNVAVDLKEAGFEILSTEGDSGEWTLVDAGDLVVHVMLPAVRDF
                     YDIDTLWGGEKPSFHAGMQKPWHAAD"
     gene            complement(1354..1959)
                     /gene="nadD"
                     /locus_tag="ACFM8S_001574"
     CDS             complement(1354..1959)
                     /gene="nadD"
                     /locus_tag="ACFM8S_001574"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFC6314627.1"
                     /translation="MKKIGLFGGTFDPIHNGHLHIARAFADEIGLDAVVFLPTGGPYH
                     KDAASASAADRLAMVELATAEDARFAVSDCDIVREGATYTFDTVQIFRQQFPSAQLWW
                     LMGSDSLMKLHTWKKWQMLVRETNIAVAMRQGDSLHQTPRELHAWLGKSLQDGSVRIL
                     SAPMHNVSSTEIRRNLAGQGVSDGIPPAAARYIREHGLYEK"
     gene            complement(1956..2555)
                     /locus_tag="ACFM8S_001575"
     CDS             complement(1956..2555)
                     /locus_tag="ACFM8S_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class carbonic anhydrase"
                     /protein_id="HFC6314628.1"
                     /translation="MSELDNILAHNRQFVESGEYEKYFTDKYPERGLAVLSCMDARII
                     GLLPDALGLKNGDAKLIKNAGALVTHPWGSVMRSLLVAVFELKVREIMVIAHHDCGMQ
                     GLNAEEFLGRVRESRIPEDRIETLRYAGVDLDGWLTGFDNVEDSVRHTVELIRRHPLM
                     PRHIAVHGLVIHPVTGKLTLVVDGSVSDGMDLSEGMETS"
     gene            complement(2584..4125)
                     /locus_tag="ACFM8S_001576"
     CDS             complement(2584..4125)
                     /locus_tag="ACFM8S_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC6314629.1"
                     /translation="MDGRRWAVWGAFALLPSAFLAVMVVAPLWAVAAYDGLAWRAVLS
                     DAYMLKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTLV
                     AGVGVLALFGADGLLWRGRQDTPYLLLYGNVFFNLPVLVRAAYQGFVQVPAARLQTAR
                     TLGAGAWRRFWDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQL
                     VMFELDMAVASVLVWLVLGVTAAAGLLYAWFGRCAVSDKAVSPVMPSPPQSVGEYVLL
                     AFAAAVLSVCCLFPLLAIVVKAWSADESWRVLMESETWQAVWNTLRFSAAAVYAAAVL
                     GVVYAAAARRSAWMRGLMFLPFMVSPVCVSAGVLLLYPQWTASLPLLLAMYALLAYPF
                     VAKDVLSAWDALPPDYGRAAAGLGANGFQTAYRITFPLLKPALRRGLTLAAATCVGEF
                     AATLFLSRPEWQTLTTLIYAYLGRAGADNYARAMVLTLLLSAFALGIFLLLDGGEGGK
                     QTETL"
     gene            4364..5749
                     /locus_tag="ACFM8S_001577"
     CDS             4364..5749
                     /locus_tag="ACFM8S_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HFC6314630.1"
                     /translation="MDGWTQTLSAQTLLGISAAAIILILILIVKFRIHALLTLVIVSL
                     LTALATGLPTGSIVNDVLVKNFGGTLGGVALLVGLGAMLGRLVETSGGAQSLADALIR
                     MFGEKRAPFALGVASLIFGFPIFFDAGLIVMLPIVFATARRMKQDVLPFALASIGAFS
                     VMHVFLPPHPGPIAASEFYGANIGQVLILGLPTAFITWYFSGYMLGKVLGRTIHVPVP
                     ELLSGGTQDNDLPKEPAKAGTVVAIMLIPMLLIFLNTGVSALISEKLVSADETWVQTA
                     KIIGSTPIALLISVLVALFVLGRKRGESGSALEKTVDGALAPVCSVILITGAGGMFGG
                     VLRASGIGKALADSMADLGIPVLLGCFLVALALRIAQGSATVALTTAAALMAPAVAAA
                     GFTDWQLACIVLATAAGSVGCSHFNDSGFWLVGRLLDMDVPTTLKTWTVNQTLIALIG
                     FALSALLFAIV"
     gene            5769..6287
                     /locus_tag="ACFM8S_001578"
     CDS             5769..6287
                     /locus_tag="ACFM8S_001578"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase, GntK/IdnK-type"
                     /protein_id="HFC6314631.1"
                     /translation="MTTHFVVMGVCGCGKTTAALSLQKHLGQCPYAEGDEFHTQANRD
                     KMGAGIPLTDEDRYPWLGNLRDWMTQQAQNGANHTIVTCSALKRGYRDILRGAEGKAA
                     FIHLSPPQDINLERMMSRKGHYMKAGMLDSQLEILEELGESEYGVKIANPGTPEAVEA
                     DILNWVASENLL"
BASE COUNT         1408 a         1985 c         1683 g         1287 t
ORIGIN      
        1 cgtctgaagc agtttcggac agcctgtgtt caaaacggaa aaccgttatt gcggaacgta
       61 tccctgaacg gcatccgcgc cgtcgccgaa gaaatactgc tccatctgct gagccaggta
      121 ttcgcgcgcg cgcggatcgg cgaggcttaa acggttttcg ttaatcagca tcgtttggtg
      181 gcgcgtccac gccgcccacg cttcttgcga tacgttttca aaaatgcgct tgcccaattc
      241 gttgggaagc ggcggaaatt tcatgccttc ggcttctttg ttgagcttga cgcagaatac
      301 catgcgtgcc atagcggatt cctttgctgt gttcagaaat aacggggtga ttttaaccga
      361 ttagggatac ggacaaaagc cttcttattc ccgatgatag ggatggttgt gcaggatgga
      421 aacggcgcgg tagagctgct cggtcagaaa gacgcgcacc atgccgtgcg gcagggtgag
      481 gctggacagg cgcatcatca tgcgggcttg ttgtttgagg cggtcggtca tgccgtccgc
      541 gccgccgatg acgaagcaga cgtgttcgcc gttttgctgc caacttttga ggtgttccgc
      601 cagctcgacg gaggtcggtg ctttgccgcg ttcgtcaaga acgacgagga acgcgccttg
      661 cggaatggct tcaaggatgc gtttttcttc cgccgccata ccttgggcgg cattcacgcc
      721 cgcgccgcgt ttttcgggtt tgatttcttt gagtgcgtag gcgacgtcgc gtccgaagcg
      781 tttggcgtat tcggcgacgg cctcatcaac ccagcgcggc attttggtgc cgactgccaa
      841 aacggtgatg ttcaatgctt tctcccttac aggaaaatgc cgtctgaaca ttcagacggc
      901 atcgggaatc agtctgccgc gtgccacggc ttctgcattc cggcgtggaa actcggtttc
      961 tcgccgcccc agagggtgtc gatatcatag aagtcgcgca cggcggggag cataacgtgg
     1021 acgacgaggt ctcctgcatc aacgagcgtc cattcgccgc tgtctccttc ggtactgagg
     1081 atttcaaaac cggcttcttt caaatcgacg gcaacgttgt tggccagtgc tttgacttgg
     1141 cgcgtactgt cgccgctggc gataatcatt ctggcaaaca gcgaagtttt gtcttgcgtt
     1201 tcgagaacgg aaatgtcttt ggctttgatg tcttcgaggg cgttgacggc gaccccgacc
     1261 attttttgca ggtcttgcag ttcttgttcg ttcattatgt tcctaacggg atgttttcag
     1321 acggcattat agccgtttct tactgatttg actttatttt tcatacaaac cgtgttcgcg
     1381 gatgtagcgt gcggcggcag gcgggatgcc gtctgaaacg ccttggccgg caaggttgcg
     1441 gcggatttcc gttgacgaca cattatgcat cggggcggac aagatgcgga cgctgccgtc
     1501 ctgaagggac ttgcccagcc acgcgtgcag ttcgcgcggg gtttggtgca ggctgtcgcc
     1561 ctgcctcatg gcgacggcga tattggtttc gcgcacgagc atctgccatt ttttccatgt
     1621 gtgcagcttc atcaggctgt cgctgcccat cagccaccag agttgcgcgg atgggaactg
     1681 ctggcggaag atttggacgg tatcaaaagt ataggttgca ccttctcgga cgatgtcgca
     1741 atcgctgacg gcaaaacgcg cgtcttctgc cgtcgccagt tcgaccatgg caaggcggtc
     1801 ggcggcggaa gcggaggctg cgtctttgtg atacgggccg cctgtcggca ggaaaacaac
     1861 cgcgtccaag ccgatttcgt cggcaaaggc gcgggcgatg tggagatgcc cgttgtgtat
     1921 cgggtcgaaa gtgccgccga acagtccgat tttcttcatg atgtttccat tccttccgat
     1981 aagtccatgc cgtctgaaac actgccgtcc acaaccagcg tcagtttgcc ggtaacggga
     2041 tggatgacca atccgtgaac ggcaatatgg cgcggcatca gcggatggcg gcggatgagt
     2101 tcgacagtat ggcgcacgct gtcttcgacg ttgtcgaaac cggtcagcca gccgtcgagg
     2161 tcgacacctg cataacgcag ggtttcgata cggtcttcgg gaatccggct ttcccggacg
     2221 cgcccgagga attcttcggc attcagcccc tgcataccgc aatcgtgatg ggcgatgacc
     2281 ataatctctc tgaccttcaa ttcaaacacg gcaaccaaaa gactccgcat caccgaaccc
     2341 cacgggtgcg taaccagcgc gccggcattt ttaatcagct tggcatcgcc gtttttcaaa
     2401 cccaacgcgt cgggcagcag cccgataatc cgcgcatcca tacaggacaa aactgccagc
     2461 ccgcgttcgg ggtatttgtc ggtaaagtat ttttcatatt cgcccgactc gacaaactgc
     2521 cggttatggg caaggatgtt atccaactcg ctcattttgc cgtcctctga aaaagggttc
     2581 acattataac gtttccgtct gttttccgcc ttcgccgccg tccaacagca ggaaaatgcc
     2641 cagcgcgaac gccgacaaca gcaatgtcaa caccattgcc cgcgcgtaat tgtccgcacc
     2701 cgcgcgtccc aaataggcat aaatcaaagt cgtcagcgtc tgccattccg gacgcgacag
     2761 gaacaatgtc gccgcaaatt cgcccacgca ggttgccgcc gccaaagtca gaccgcgccg
     2821 caacgccggt ttcaaaaggg ggaacgtgat gcggtatgcc gtctgaaagc cgtttgcacc
     2881 caaacccgcc gccgccctgc cgtaatccgg cggcagtgca tcccaggctg ataaaacatc
     2941 ttttgccaca aacggatacg ccagcagcgc atacatcgcc agcagcagcg gcaacgaagc
     3001 cgtccactgc ggataaagca gcagcacgcc cgccgaaaca caaaccggcg acaccataaa
     3061 cggcaaaaac atcagcccgc gcatccacgc cgaccgccgc gccgccgccg catacaccac
     3121 acccaaaacc gccgccgcat acaccgccgc cgccgagaag cgcaaagtat tccacaccgc
     3181 ctgccacgtt tcactttcca ttaacacacg ccacgattcg tcggccgacc acgctttcac
     3241 aacaattgcc aacaaaggaa acaggcagca cacagacaac accgccgccg caaacgccag
     3301 cagcacatat tccccgaccg actgcggcgg cgacggcatc acaggggaaa ccgccttatc
     3361 cgaaaccgcg cacctgccga accacgcata cagcaaccct gccgccgccg ttacccccaa
     3421 caccagccac accagcaccg aagcaaccgc catatcgagt tcgaacatga ccaactggta
     3481 aatttccact tcgaccgtgg cataacggct gccgcccagc agcaatgcca gcccgaaccc
     3541 cgaaaaacaa tacaggaaga caaggcacac gccgccggca agccacgggc gcaaaacggg
     3601 catttcaatg tcccaaaacc gccgccacgc ccccgcgccc aacgtccgtg ccgtctgaag
     3661 ccgtgccgca ggcacttgca caaacccctg atacgccgcc ctgaccaaca caggaaggtt
     3721 gaaaaacaca ttgccgtaca acaacagata cggcgtatcc tgccgtccgc gccacaacag
     3781 gccgtccgcc ccaaacagag ccagcacgcc cacgcccgcc accaacgtgg gcatcacaaa
     3841 aggcagcatc agcaggcgca gcaccaaagc ccgccccgga aacgccagcc gcgccagcac
     3901 ccacgcgaca ggcacgccca aaggcagcac cagcacacag gttgccgctg cctgaaatac
     3961 cgtccacgcc aaacgtttga gcatataggc atccgacagc accgcgcgcc acgccaaacc
     4021 gtcatacgcc gccaccgccc acaaaggcgc aacgaccatt accgccaaaa aagccgaagg
     4081 cagcagggca aaagcacccc ataccgccca acgccgtcca tccatcgcct tccccacttg
     4141 aaacactgat gttgcgattg tacccaaaag ccccacatac cgtatatttc aatccgacta
     4201 cataccgtat ccgccttcct cccgccgtct gaaataaatc gttcaaataa acaggaatat
     4261 aacttcagac aaacaactta ccgcccgatt tgtgctatcg ttttcgcaca acttaaaaaa
     4321 acctgacaat tttgtacttt tattacagag aaaggcttta caaatggacg gctggacaca
     4381 gacgctgtcc gcgcaaaccc tgttgggcat ttcggcggcg gcaatcatcc tcattctgat
     4441 tttaatcgtc aaattccgca tccacgcgct gctgacactg gtcatcgtca gcctgctgac
     4501 ggctttggca accggtttgc ccacaggcag cattgtcaac gacgtactgg tcaaaaactt
     4561 cggcggcacg ctcggcggcg tggcgcttct ggtcggcctg ggcgcgatgc tcggacgttt
     4621 ggtcgaaaca tccggcggcg cacagtcgct ggcggacgcg ctgatccgga tgttcggcga
     4681 aaaacgcgca ccgttcgcgc tgggcgttgc ctcgctgatt ttcggcttcc cgattttctt
     4741 cgatgccgga ctaatcgtca tgctgcccat cgtgttcgcc accgcacggc gcatgaaaca
     4801 ggacgtactg cccttcgcgc ttgcctccat cggcgcattt tccgtcatgc acgtcttcct
     4861 gccgccccat ccgggcccga ttgccgcttc cgaattttac ggcgcgaaca tcggccaagt
     4921 tttgattttg ggtctgccga ccgccttcat cacatggtat ttcagcggct atatgctcgg
     4981 caaagtgttg gggcgcacca tccatgttcc cgttcccgaa ctgctcagcg gcggcacgca
     5041 agacaacgac ctgccgaaag aacctgccaa agcaggaacg gtcgtcgcca tcatgctgat
     5101 tcccatgctg ctgattttcc tgaataccgg cgtatcggcc ctcatcagcg aaaaactcgt
     5161 aagtgcggac gaaacctggg ttcagacggc aaaaataatc ggttcgacac cgatcgccct
     5221 tctgatttcc gtattggtcg cactgtttgt cttgggacgc aaacgcggcg aaagcggcag
     5281 cgcgttggaa aaaaccgtgg acggcgcact cgcccccgtc tgttccgtga ttctgattac
     5341 cggcgcgggc ggtatgttcg gcggcgtttt gcgcgcttcc ggcatcggca aggcactcgc
     5401 cgacagcatg gcggatttgg gcattcccgt ccttttgggc tgtttccttg tcgccttggc
     5461 actgcgtatc gcgcaaggtt cggcaaccgt cgccctgacc accgccgccg cgctgatggc
     5521 tcctgccgtt gccgccgccg gctttaccga ctggcagctc gcctgtatcg tattggcaac
     5581 ggcggcaggt tcggtcggtt gcagccactt caacgactcc ggcttctggc tggtcggccg
     5641 cctcttggac atggacgtac cgaccacgct gaaaacctgg acggtcaacc aaaccctcat
     5701 cgcactcatc ggctttgcct tgtccgcact gctgttcgcc atcgtctgac agacggaaag
     5761 gatagtaaat gactacgcat tttgtcgtta tgggcgtatg cggctgcggc aagaccaccg
     5821 ccgcgctgtc cctgcagaaa cacctcggtc aatgtcccta tgccgaaggc gacgagttcc
     5881 acacccaagc caaccgcgac aagatgggcg cgggtattcc gctgaccgat gaagaccgct
     5941 atccgtggtt gggcaatctg cgcgactgga tgacgcaaca ggcgcaaaac ggtgcgaacc
     6001 acaccatcgt aacctgttcc gccctcaaac gcggctaccg cgacattctg cgcggagccg
     6061 aaggcaaagc tgccttcatc cacctcagtc cgccgcaaga catcaacctc gagcgcatga
     6121 tgtcgcgcaa aggacattac atgaaagcag ggatgctcga ttcgcaactg gaaatcctcg
     6181 aggaactggg cgaaagcgaa tacggggtca aaatcgccaa ccccggcacg cccgaagcgg
     6241 tcgaagccga tattctgaac tgggttgcct cggaaaacct gctttgaagc aatatgccgt
     6301 ctgaagcccg acacaggatg ggtttcagac ggcataaaca tcgggaacag aatggattac
     6361 att
//
LOCUS       DAXHSF010000120         6351 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.120, whole genome shotgun sequence.
ACCESSION   DAXHSF010000120 DAXHSF010000000
VERSION     DAXHSF010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6351
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.120"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(387..1604)
                     /gene="nqrF"
                     /locus_tag="ACFM8S_001579"
     CDS             complement(387..1604)
                     /gene="nqrF"
                     /locus_tag="ACFM8S_001579"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HFC6314632.1"
                     /translation="MEIILGIVMFTVIVLVLALMILFAKSKLVSEGDITIKVNGEKEL
                     TMPAGGKLLGALANEGIFIPSACGGGGSCGQCRVVVKSGGGDILPTELSHISKREARE
                     GCRLSCQVSVKTDMDIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
                     AGGYIQIEAPPHTVAYKDFDIPKEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEK
                     GIIMLNVRIATPPPRVPDAPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFI
                     GGGAGMAPMRSHIFDQLKRLNSKRKITFWYGARSKREMFYVEDFDQLAAEFPNFTWHV
                     ALSDPLPEDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLG
                     VEDENILLDDFGG"
     gene            complement(1618..2211)
                     /gene="nqrE"
                     /locus_tag="ACFM8S_001580"
     CDS             complement(1618..2211)
                     /gene="nqrE"
                     /locus_tag="ACFM8S_001580"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HFC6314633.1"
                     /translation="MEHYLSLFIKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGVA
                     VIFVLGLSVPVNQLVYSLLKDGAIVEGVDLTFLKFITFIGVIAALVQILEMFLDKFVP
                     ALYNALGIYLPLITVNCAIFGAVSFMAQREYNFGESVVYGFGAGLGWMLAIVALAGIT
                     EKMKYSDAPKGLKGLGITFIAAGLMAMAFMSFSGIQL"
     gene            complement(2215..2841)
                     /locus_tag="ACFM8S_001581"
     CDS             complement(2215..2841)
                     /locus_tag="ACFM8S_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HFC6314634.1"
                     /translation="MADMKRLKHLMFSPFIDNNPIALQVLGICSALAVTTKLQTAIVM
                     GISVALVTGFSSFFISLVRNYIPNSIRIIVQMAIIASLVTLVDQLLQAFAYELSKQLS
                     VFVGLIITNCIVMGRAEAFAMKEPPLESLIDGIGNGAGYGIMLLVVATVRELIGSGKL
                     LGYTVFQTVQDGGWYQTNGLFLLAPSAFFIIGFLIWGLRTWKPEQAEE"
     gene            complement(2841..3617)
                     /locus_tag="ACFM8S_001582"
     CDS             complement(2841..3617)
                     /locus_tag="ACFM8S_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HFC6314635.1"
                     /translation="MAKKFDKDSFSGTLIVVLAVSLICSVIVAGAVVGLKPIQEKQKL
                     QDKQGYILSVAGLMDKDTDIGKTFAERIEQRVVDLATGEYVADAPKDFSARIAGKDPA
                     QSIRIKTEDDLAGIKSRAKYTEVYLVKGEDGKIGQIILPMHGNGLWSVMYGFVAIQPD
                     GNTINGITYYEQGETPGLGGEIGNPLWQQKFVGKKLFDGQGKLALHVGKGAGSDKEHG
                     VDALSGASLTSKGVQGSFAYWFGENGYIPYLNKLKSAGAQ"
     gene            complement(3610..4842)
                     /locus_tag="ACFM8S_001583"
     CDS             complement(3610..4842)
                     /locus_tag="ACFM8S_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HFC6314636.1"
                     /translation="MGLKHFLEKIEPHFLPGGKHEKWYALYEAAATIFYTSGAVTRKA
                     AHVRDALDSKRMMILVWLALFPAMFYGMYNVGVQAFDALTPDLLQQSIANDWHYALAD
                     ALGINMSSEAGVLGKMLFGAIYFLPIYATVFVVGGFWEVLFATVRKHEINEGFFVTSI
                     LFALIVPPTLPLWQAALGISFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSG
                     DAVWTAVDGYSGATALAQWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLAL
                     LIGGAFIVFARIASWRIIAGVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAI
                     GMLFMATDPVSASFTNVGKWWYGALIGVMCVLIRVVNPAYPEGMMLAILFANLFAPIF
                     DYFVAQANIKRRKARSNG"
     gene            complement(4845..6188)
                     /locus_tag="ACFM8S_001584"
     CDS             complement(4845..6188)
                     /locus_tag="ACFM8S_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HFC6314637.1"
                     /translation="MIKIKKGLDLPIAGRPEQVIYDGPAITEVALLGEEYAGMRPSMK
                     VKEGDAVKKGQVLFEDKKNPGVVFTAPASGKIAAIHRGEKRVLQSVVIAVEGDDEIEF
                     ERYAPDALAKLSGEEVRRNLIQSGLWTALRTRPFSKIPAVDAEPFAIFVNAMDTNPLA
                     ADPVVVIKEAAEDFKRGLLVLSRLTEHKIHVCKAAGADVPSENAANIETHEFGGPHPA
                     GLSGTHIHFIEPVGANKTVWTINYQDVIAIGHLFITGRLNTGRVIALGGSQVNKPRLL
                     RTVLGAKVSQITAGELVDADNRVISGSVLNGAIAQGAHDYLGRYHNQISVIEEGRSKE
                     LFGWVAPQPDKYSITRTTLGHFLKNKLFKFTTAVNGGDRAMVPIGTYERVMPLDILPT
                     LLLRDLIVGDTDSAQALGCLELDEEDLALCSFVCPGKYEYGPLLRKVLETIEKEG"
BASE COUNT         1526 a         1641 c         1674 g         1510 t
ORIGIN      
        1 aaccggtgtt ttaccaaccg gtttgtcaag gtttaatggt cagcgcgtcc tgcgttgcca
       61 tttattgtag tagtattcgt aatcttcatc acgaccgccc gattcaatcc aatcatcgta
      121 ttcggggctg ttttcaaagc tgtcagacat tatccgtacc tcgcaaaatg ggtttatcga
      181 tactttttta gttatggaat caaccataac taaaaagaag tatacttctt tgcttttccg
      241 ccagaatatt taaccgcaca tccgccgcag ggtaaaaatg gaagttcaac gatattgata
      301 ctggtatggg cggatgccgt ctgaagggtg ttcggacggt atcggataaa aaaaggaaga
      361 aaccgaagtt tcttcctttt tgtctgttag ccgccgaaat cgtccaagag gatgttttcg
      421 tcttccacac ccaagtcttt gagcattttg atgacggact ggttcataat cggaggcccg
      481 cacatataaa attcgcagtc ttccggcgct tcgtgatttt tcaggtggtt ttcgtaaacc
      541 acgttgtgaa tgaagcctgt gtaaccgtcc cagttgtctt caggcagcgg atcggacagg
      601 gcgacgtgcc acgtgaagtt cgggaattct gccgcgagtt ggtcgaagtc ttcgacatag
      661 aacatctcgc gtttggaacg tgcaccatac cagaaggtaa ttttgcgttt ggagttcaaa
      721 cgtttcagct ggtcgaaaat gtgggaacgc atcggagcca tacccgcgcc gccgccgata
      781 aataccattt cggcatcggt atctttggca aagaattcgc caaacggacc ggaaatcgtt
      841 actttatcac cgggtttgag cgaccagatg taggacgaca tttgtcccgg aggcgcatca
      901 ggtacgcgtg ggggcggcgt ggcgatacgc acgttcagca taatgatgcc tttttcttca
      961 gggtatgaag ccatagagta ggcgcgcaaa atcggctcgt ccactttgga aacgtattgc
     1021 cacaaattgt atttgtccca gtcttcgtga tattccttag gaatgtcgaa gtctttgtag
     1081 gcaacagtgt gaggaggagc ttcaatttga atgtagccgc cggcgcggaa ggggacttct
     1141 tcgccttcgg gaatggcaag cttgagttct ttaatgaacg tggctttgtt atcgttggag
     1201 atgacggtgc attcccattt tttcacgccg aacacttctt cgggcacttc gatgtccatg
     1261 tcggttttga cgctgacttg gcacgacaga cggcagcctt cgcgtgcttc gcgtttgctg
     1321 atgtgggaca gctcggtcgg caggatgtcg ccgccgccgc tttttacgac gacgcggcat
     1381 tgtccgcacg aaccgccccc gccgcaggcg gaggggataa agatgccttc gttggcaagc
     1441 gcgcccaaga gtttgccgcc ggcgggcatc gtcagctctt tttcgccgtt gactttgatg
     1501 gtgatgtcgc cttcgctgac cagtttggat ttggcaaata gaatcatcag tgccaaaacc
     1561 aaaacgatga cggtaaacat cacgatacct aaaataatct ccataccggt ccctttctta
     1621 taactggatg ccggagaacg acataaacgc catcgccatc aggccggcgg cgataaaggt
     1681 aatgcccaag cctttgaggc ctttgggagc gtccgaatat ttcatttttt cggtaatgcc
     1741 cgccaaagcg acaatcgcca acatccagcc caagcccgcg ccgaagccgt atacaacgga
     1801 ctcgccgaag ttgtattcgc gttgcgccat aaacgatacg gcgccgaaaa tcgcgcagtt
     1861 cacggtaatc agcggcaggt agatgcccaa cgcgttatag agggcgggga cgaatttatc
     1921 caagaacatt tccaaaatct gcaccaaagc ggcaatcacg ccgatgaagg tgatgaattt
     1981 caaaaaggtc aaatccacgc cttcgacaat cgcgccgtct ttgagcagcg agtaaacgag
     2041 ttggttgaca gggacggaca gcccgagtac gaaaattacc gccacaccca aaccgaatgc
     2101 ggtggatact tttttggata ccgccaaaaa cgtgcacata cccaaaaaga aggatagtgc
     2161 catattttca atgaagacgg atttgatgaa gaggctcaaa tagtgttcca tagcttattc
     2221 ctccgcctgt tcgggtttcc aggtacgcag tccccaaatc aaaaagccga tgatgaagaa
     2281 cgcgctgggg gcgagcagga acaagccgtt ggtctgatac cagccgccgt cctgcacggt
     2341 ttggaaaacg gtgtagccca agagtttgcc cgagccaatc agttcgcgga cggtggcgac
     2401 gacaagcagc attatcccgt agcccgcgcc gttgccgatg ccgtcgatca ggctttccag
     2461 cggcggctct ttcatcgcaa atgcttcggc gcggcccatc acgatacagt tggtaataat
     2521 cagaccgacg aatacggaaa gctgtttgga caattcgtag gcaaatgcct gcaagagttg
     2581 gtcgaccagc gtaaccagcg acgcgataat cgccatttgc acgataatac ggatgctgtt
     2641 ggggatgtag ttgcgtacca gcgaaatgaa gaagctggaa aaaccggtta ccaaagctac
     2701 ggaaataccc atcacgatgg ccgtctgaag tttggtggta accgccaaag ccgaacaaat
     2761 acccaaaacc tgcaaggcaa tcgggttgtt gtcgataaag ggtgaaaaca tcaaatgttt
     2821 caagcgtttc atatcagcca ttattgcgct cctgctgatt tcaatttgtt caggtagggg
     2881 atatagccgt tttcgccgaa ccagtaggcg aacgaacctt gcacgccttt ggatgtcagc
     2941 gatgcgccgg agagggcatc tacgccgtgt tctttgtccg aacccgcgcc tttgccgacg
     3001 tgcagggcga gtttgccttg tccgtcaaac agttttttgc cgacgaattt ttgctgccac
     3061 aacggattgc cgatttcgcc gcccaagccc ggggtttcgc cttgttcgta gtaggtaatg
     3121 ccgttgatgg tgttgccgtc gggctggatg gcgacaaagc cgtacatgac tgaccacaaa
     3181 ccgttgccgt gcataggcag gatgatttgc ccgattttgc cgtcttcgcc ttttaccaaa
     3241 taaacctcgg tgtatttggc acggcttttg atgcctgcca aatcgtcttc ggttttgatg
     3301 cggatgcttt gggcagggtc tttgcctgcg atgcgcgcgc tgaagtcttt aggcgcatcg
     3361 gcgacgtatt cgccggtcgc caaatcgaca acacgttgct cgatacgctc ggcaaaggtt
     3421 ttaccgatgt cggtgtcctt atccatcaaa ccggctacgc tcaagatata gccttgtttg
     3481 tcttggagtt tttgtttctc ttggatgggt ttcaagccga cgaccgcacc ggcaacgatg
     3541 accgagcaaa tcaggctgac cgccaacacg acaatcagcg tgccgctgaa gctgtcttta
     3601 tcgaatttct tagccattgc tgcgcgcctt tctgcgtttg atgttcgctt gtgcgacgaa
     3661 atagtcgaaa atcggggcaa acaggttggc aaacagaatc gccaacatca tgccttcggg
     3721 gtaagccgga ttgaccacgc ggattaatac gcacatcaca ccgatcagtg cgccgtacca
     3781 ccatttgccg acattggtaa aggaagcgga aacagggtcg gtcgccataa acagcatacc
     3841 gatggcgaag ccgccgacca ccaagtgcca gtaccaaggc atagcaaaca tagcgttggt
     3901 gtccgaaccg atgaagttga acagcgaaga catcgcaatc ataccgatca tcacgccggc
     3961 aataatgcgc caagaagcga tgcgggcaaa cacgataaac gcgccgccga ttaagagtgc
     4021 caaagtggag acttcgccga tggagccggg cagtttgccg ataaacgcgt ccatccaagt
     4081 gatggtttga ccggttacgg cgtttttcag gccgtctgca ccgtgtgccg cccattgcgc
     4141 cagcgcggtt gcgccggaat agccgtcaac cgccgtccaa accgcatcgc cgctcaagtt
     4201 ggcggggtag gcgaagaaca agaaagcgcg gcctgccagc gcagggttca taaagttttt
     4261 acccgtaccg ccgaatactt ctttcgcaac cacaacgccg aaagaaatac ccaaagccgc
     4321 ctgccacagc ggcagcgtgg gcggaacgat taaggcaaac agaatcgagg taacaaagaa
     4381 accttcgttg atttcgtgtt tgcgtacggt ggcaaacaag acttcccaaa aaccgcccac
     4441 aacaaagaca gtcgcgtaaa tcggcagaaa ataaatcgcg ccgaacagca ttttgcccaa
     4501 tacgcccgct tcagacgaca tattgatgcc caaagcgtcg gcaagggcgt aatgccagtc
     4561 gttggcgatg ctttgttgca gcaaatcggg cgttaacgca tcgaacgcct gtacgccgac
     4621 gttgtacata ccgtaaaaca tggcggggaa caaagccagc cacaccaaaa tcatcatgcg
     4681 cttggagtcg agcgcgtcgc ggacgtgtgc cgctttgcgc gttaccgcgc cggatgtata
     4741 gaaaatcgtc gccgcagctt cgtagagggc ataccatttt tcatgtttgc cgcccggcag
     4801 gaagtgcggt tcgatttttt ccagaaaatg tttcaagccc ataatcagcc ttccttctca
     4861 atggtttcca gcaccttacg caacagcggg ccgtattcgt atttgcccgg gcagacgaag
     4921 ctgcacaaag cgaggtcttc ctcgtccaat tccaagcaac ccaaagcctg cgcgctgtcg
     4981 gtatcgccga cgattaaatc gcgcagaagc agggtaggca ggatgtccag cggcatcacg
     5041 cgctcgtaag taccgatggg caccatagcg cggtcgccgc cgttgacggc tgtcgtgaac
     5101 ttgaagagtt tgtttttcag gaaatggccg agggttgtac gcgtaatcga gtatttgtcc
     5161 ggctgcggcg caacccagcc gaacagctct ttgctgcggc cttcttcgat aacggaaatc
     5221 tgattgtggt agcgtcccaa ataatcatgc gcgccttgtg caatcgcgcc gttcaatact
     5281 gagccggaaa tcacgcggtt gtctgcgtca accaattcgc ccgcagtaat ttgcgatact
     5341 ttcgcaccca aaacggtacg caagaggcgc ggtttgttga cttgagaacc acccaaggca
     5401 atcacgcgcc cggtattcag acggcctgtt atgaataaat gtccgatggc aatcacgtct
     5461 tgataattga tggtccaaac agttttgttt gcaccgactg gctcgatgaa atgaatgtgc
     5521 gtgccgctca aaccggcagg atgcgggccg ccaaattcat gtgtttcaat gttggcggcg
     5581 ttttcagacg gcacgtctgc gcctgctgct ttacacacat ggattttatg ttcggtcagg
     5641 cggcttaata ccaacagacc gcgtttgaag tcttcggcgg cttctttgat aacgaccacg
     5701 ggatcggcag ccagcggatt ggtgtccatc gcattgacga agatggcgaa cggctcggca
     5761 tcgacggcgg gaattttgct gaacggacgg gtacgcagcg cggtccacaa accggattga
     5821 atcagattgc ggcgcacttc ttcgccgctc aattttgcca acgcatcggg cgcgtagcgt
     5881 tcgaactcga tttcgtcatc gccttcaacg gcaatcacaa ccgactgaag cacgcgcttt
     5941 tcgccacggt gaatcgcggc gattttgccg gaagccggcg cagtaaatac cacgcccgga
     6001 ttctttttgt cttcaaacag cacttggcct tttttgacgg catcgccttc cttgactttc
     6061 atcgaggggc gcataccggc atattcttcg ccaagcaacg cgacttcggt aatggccggg
     6121 ccgtcataaa tgacttgctc cggtctgcct gcgatgggca ggtctagacc ttttttgatt
     6181 ttaatcatag atttgcatta cttgtgatgg ttaaggtaaa aacggcgtgt tttgataccg
     6241 tgtcgcgcgg catcaaaagc attgaataaa ttaatgtagc aaagtgctag attttatcag
     6301 gaattgtacc tgtttgtcag atttgctgct tttttccttg cggaagccgt t
//
LOCUS       DAXHSF010000121         6118 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.121, whole genome shotgun sequence.
ACCESSION   DAXHSF010000121 DAXHSF010000000
VERSION     DAXHSF010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6118
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.121"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(152..2080)
                     /gene="dnaK"
                     /locus_tag="ACFM8S_001585"
     CDS             complement(152..2080)
                     /gene="dnaK"
                     /locus_tag="ACFM8S_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFC6314638.1"
                     /translation="MAKVIGIDLGTTNSCLAISENGQTKVIENAEGARTTPSVIAYLD
                     GGEILVGAPAKRQAVTNAKNTIYAAKRLIGHKFEDKEVQRDIESMPFEIIKANNGDAW
                     VKAQGKELSPPQISAEVLRKMKEAAEAYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGMDKGDNKDRKVAVYDLGGGTFDISIIEIANLDGDKQFE
                     VLATNGDTFLGGEDFDQRLIDHIIAEFKKEQGIDLKQDVMALQRLKEAAEKAKIELSS
                     GQQTEINLPYITMDATGPKHLAMKITRAKFESLVEDLITRSIEPCKIALKDAGLSTGD
                     IDDVILVGGQSRMPKVQEAVRDFFGKEPRKDVNPDEAVAVGAAIQGEVLSGGRSDVLL
                     LDVTPLSLGIETMGGVMTKLIQKNTTIPTKASQVFSTAEDNQSAVTIHVLQGERERAS
                     ANKSLGQFNLGDIAPAPRGMPQIEVTFDIDANGILHVSAKDKGTGKAANITIQGSSGL
                     SEEEIERMVKDAEANAEEDKKLTELVASRNQAEALIHSVKKSLADYGDKLDAAEKEKI
                     EAALKEAEEAVKGDDKAAIDAKAEALGTASQKLGEMVYAQAQAEAQAGESEQANASAK
                     KDDDVVDADFEEVKDDKK"
     gene            complement(2288..2560)
                     /locus_tag="ACFM8S_001586"
     CDS             complement(2288..2560)
                     /locus_tag="ACFM8S_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314639.1"
                     /translation="MKKTGKYLIYAAAFTAFCFAFQENRSEAKQPDITLSASLCEQFN
                     ILNAKDMDAEQVSLSKECDIIESSHDWEKEYGNLNEQEMLAGIVYE"
     gene            2746..3432
                     /locus_tag="ACFM8S_001587"
     CDS             2746..3432
                     /locus_tag="ACFM8S_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6314640.1"
                     /translation="METFKDRLVFLWKSEAKQAKIASDIEMTIAGFSRIWNEGGLPKS
                     ETLKKIKQLKGCSIDWLLTGEGNPFPDEAPKKSLAYDTLGNEVDTDEFVFVPRYDIRA
                     AAGYGQFVDHEEPVFTMAFRRHWIENYVTRDTKNLSVISVKGDSMEGVLNDGDSILVN
                     HGENTPRDGLYVLRINENLLVKRLQIVPGGIINVISANEAYPAFEINLNDLTDDVEII
                     GRVEWFGRTI"
     gene            3564..3902
                     /gene="erpA"
                     /locus_tag="ACFM8S_001588"
     CDS             3564..3902
                     /gene="erpA"
                     /locus_tag="ACFM8S_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFC6314641.1"
                     /translation="MSDESPIIFTDSCCAKVADLIAEENNPDLKLRVFVNGGGCSGFQ
                     YGFTFDEIKNDDDFEIEKNGLVFLVDPMSYQYLVGAEIDYTESLQGSQFVIRNPNAET
                     TCGCGSSFSV"
     gene            3919..4065
                     /locus_tag="ACFM8S_001589"
     CDS             3919..4065
                     /locus_tag="ACFM8S_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314642.1"
                     /translation="MPSERSDGIFTFGKYIIGMNSHIIRLFEDESGFPKGTGGTVSGV
                     FVPL"
     gene            4062..4526
                     /locus_tag="ACFM8S_001590"
     CDS             4062..4526
                     /locus_tag="ACFM8S_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFC6314643.1"
                     /translation="MIEMSKDYRNDLYDVYVSYPPQVDRGLIRECLRENLGEEKAEGL
                     IESLDSKPQVLVEEKCTWAKREELHDYFSYLGLDIITRRYMELETVVPPEEGEGEGEG
                     ADGEMPEYLELHGGREDDISAPSQPEPPTRNIKPLLFGLLIAFLGYLLGKIF"
     gene            4561..6072
                     /gene="ubiB"
                     /locus_tag="ACFM8S_001591"
     CDS             4561..6072
                     /gene="ubiB"
                     /locus_tag="ACFM8S_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFC6314644.1"
                     /translation="MKWLKRLTVIVGTFYRYRLAGLCASLMGSGWICALLKMMPQSSK
                     LKNEPPAVCLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLS
                     REQIEKSLGQPIEKLYAEFETEPIASASIAQVHKARLHSGEQVAVKVLRPNLLPVIEQ
                     DLSLMRFGAGWVERLFADGKRLKPREVVAEFDKYLHDELDLMREAANASQLGRNFQNS
                     DMLIVPKVFYDYCTSDVLTIEWMDGTPVSDIAKLKADGIDLHKLADYGVEIFFTQVFR
                     DGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATA
                     HIESGWVPADTRAEELEAAVRAVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQ
                     LVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLVKWMNGQVGPKALWRNLKNEAPDWAQ
                     IIPSLPRKISALIDENRQQEMRDAYIHLVKVQQRQSLWLAVIAVVLLLILLLK"
BASE COUNT         1365 a         1362 c         1651 g         1740 t
ORIGIN      
        1 aaaaatttcc agagaaccga tacaacagtt ggaacttggg tttgggaata ttacggtaga
       61 tgaacttgga acctctgtta tgctatggtc ttttatctca attgaaaaaa gcgcgaatca
      121 aacggttcgc gcttttttca gacggcatca attatttttt gtcgtctttt acttcttcaa
      181 agtcggcatc tacgacatca tcgtctttct ttgcagaagc attggcttgt tcgctttcgc
      241 ctgcttgggc ttcagcttgt gcttgagcgt aaaccatttc gcccagtttt tggctggctg
      301 tgcccagtgc ttcggctttg gcatcgatgg cggctttgtc gtcgcctttc acggcttctt
      361 cggcttcttt cagcgcggct tcgatttttt ctttttcggc agcgtcgagt ttgtcgccgt
      421 agtccgccaa agattttttc acggagtgaa tcagggcttc ggcttggttg cgggaagcga
      481 ccaattcagt cagcttttta tcttcctcag cattggcttc ggcatctttc accatgcgtt
      541 cgatttcttc ttcgctcaaa cctgaagaac cttggatggt aatgttggcc gctttgccag
      601 tgcctttgtc tttggcggaa acgtgcagga tgccgttggc gtcgatgtcg aaggttactt
      661 cgatttgcgg cataccgcgc ggtgcgggtg cgatgtcgcc caagttgaac tgacccaaag
      721 atttgttggc agaagcgcgt tcgcgttcgc cttgcagtac gtggatggtt actgcgcttt
      781 ggttgtcttc ggcggtagag aacacttgcg acgctttggt cgggatggtg gtgttctttt
      841 gaatcagctt ggtcatcacg ccgcccatgg tttcgatacc caaggacaga ggggttacgt
      901 ccagtagcaa tacgtcgctg cggccgccgc tcaatacttc gccttggatc gctgcgccta
      961 cggcaacggc ttcgtcaggg ttcacgtctt tgcgcggttc tttgccgaag aagtctctca
     1021 cggcttcttg tactttcggc atacgggact gcccgccgac caagattacg tcgtcgatgt
     1081 cgccggtgct caagccggca tctttcaatg caattttgca aggttcgata gagcgggtaa
     1141 tcaggtcttc aaccaggctt tcgaatttgg cgcgggtaat tttcatcgcc aagtgtttcg
     1201 ggccggttgc gtccatggtg atgtacggca ggttaatttc ggtttgctgg ccgctggaca
     1261 attcgatttt ggctttttcg gcagcttctt tcaggcgttg tagagccatc acgtcttgtt
     1321 tcaaatcaat gccttgttct tttttgaact cggcgatgat gtggtcgatg aggcgttggt
     1381 cgaagtcttc accgcccaag aaggtatcgc cgttggttgc caatacttcg aattgtttgt
     1441 cgccgtcgag gttggcgatt tcgatgatgg aaatatcgaa agtaccgccg cccaagtcat
     1501 atacggctac tttgcggtct ttgttgtcgc ctttgtccat accgaatgcc aaagcggctg
     1561 cggtcggctc gttgatgatg cgtttcacgt ccaaaccggc gatacggcct gcgtctttgg
     1621 tggcttgacg ttggctgtcg ttgaagtagg cagggacggt aatcacggct tcggttactt
     1681 tttcgcccaa gtaagcttcg gcggcttctt tcattttacg caggacttct gcggaaattt
     1741 gaggaggaga cagctctttg ccttgtgctt ttacccatgc gtcgccgttg ttggctttga
     1801 tgatttcgaa aggcatagat tcgatgtcgc gttggacttc tttgtcttca aatttgtggc
     1861 cgatcaaacg tttggcggcg taaatagtgt ttttggcgtt ggttaccgct tggcgtttgg
     1921 caggcgcgcc gacgaggatt tcgccgccgt ccaaataagc gataacggac ggcgtggtgc
     1981 gtgcgccttc tgcgttttcg atcactttgg tttgaccgtt ttcggaaatg gccaaacaag
     2041 agttggttgt acctaagtcg ataccgatta cttttgccat gtggataatc ctatttgatt
     2101 ttgcttattt tgagaaatat gttggaacat tttgtcccga tgggctgtaa atagggcggg
     2161 cggcgggctg tttcaagcta cagcatggct ataagtatat aactttatga atatattggt
     2221 tttatatttg atttaataca tttggctcca atgcattcaa gcataatgtt tcaaatggca
     2281 ggcaggttta ttcatagacg atgccggcga gcatttcctg ttcgttcaag ttgccgtact
     2341 ctttttccca gtcgtgtgaa gactcgatga tgtcgcattc tttggaaagg gagacttgtt
     2401 ctgcatccat atctttggcg ttcagtatgt tgaattgttc gcacagggat gcggataaag
     2461 tgatgtcggg ctgtttggct tcagaacggt tttcttggaa ggcaaagcag aatgcggtaa
     2521 atgccgcagc atagataaga tatttgccgg ttttcttcat tttctatcct ttttctgtca
     2581 actcaggatt aaacctatgg aaaatcttaa aaattatgta ttgagtaaga aaaatcataa
     2641 tttaaattta gtttatcata gttgttccgt ttttgggata gctaaggtaa aatatatttc
     2701 atgtttactt tagatgattg aatgaagggg agcggaagga tatttatgga aacctttaaa
     2761 gacagactgg tttttttatg gaaaagcgaa gcgaagcagg caaaaatcgc atccgatatt
     2821 gaaatgacga ttgcgggctt cagcaggata tggaatgaag gcggtctgcc gaagtctgaa
     2881 acattaaaaa aaatcaagca gttgaagggg tgtagtatcg attggctgct gaccggggag
     2941 ggtaatccgt ttccggatga agccccaaaa aaatcccttg cttacgatac tttgggcaat
     3001 gaagtcgata cggacgagtt tgtcttcgtg ccgagatatg atattcgggc ggctgcggga
     3061 tacgggcagt ttgtcgatca tgaggaaccg gtatttacaa tggcgttcag acggcattgg
     3121 attgagaatt atgttacccg cgatacgaaa aacctgtctg taatttccgt caagggggat
     3181 tcgatggagg gggttttgaa tgacggcgat tcgattttgg tcaatcatgg tgaaaatacg
     3241 ccgagggacg gtctgtatgt gttgcggatt aatgaaaatc tgctggttaa acgtttacag
     3301 attgtaccgg gcgggattat caatgtgatt tctgcaaacg aggcttatcc tgcttttgaa
     3361 atcaatttga acgatttgac cgatgatgtg gagattatcg ggcgtgtcga gtggttcggc
     3421 aggacgattt gagtttgggg cttgaaattg caggcggtca aacttatcta ttggaacaat
     3481 tcctttttca aaggcgaagc ctgcttgcct ttgaaggggg tttgagagag aatgcagaaa
     3541 atattatatt aaggaataac accatgtcgg atgaaagccc tattattttt actgacagct
     3601 gctgtgccaa agttgccgat ttgattgccg aagaaaacaa tcccgatttg aaattgcggg
     3661 tttttgtcaa tggcggcggc tgttcgggtt tccagtacgg atttactttt gacgaaatca
     3721 aaaacgacga cgattttgaa attgagaaaa acggtttggt ctttttggtc gatccgatga
     3781 gctatcaata tctggtcggt gcggaaatcg actatacgga aagtttgcag ggttcgcaat
     3841 tcgtcatccg caatccgaat gcggaaacaa cctgcggttg cggatcgtcg ttttccgtat
     3901 gaccgcttgg tttgtgtgat gccgtctgaa cgttcagacg gcatttttac ttttggaaaa
     3961 tatattatcg ggatgaattc acatataatc cgattgtttg aagatgaatc gggtttcccg
     4021 aaaggaacgg gcggaacggt atcaggcgta tttgttccct tatgattgag atgagtaaag
     4081 attaccgaaa cgatttgtac gatgtatatg tttcctaccc gccccaagtg gatcgcgggc
     4141 ttatccggga gtgcctcagg gagaatctcg gcgaggaaaa ggcggaagga ttgatcgaat
     4201 cgctcgattc caaaccgcaa gtgctggttg aggaaaaatg cacttgggcg aaacgggaag
     4261 agttgcacga ttatttcagc tatttgggtt tggatattat tacccggaga tatatggagt
     4321 tggaaacggt cgtgccgccg gaggaaggtg agggcgaagg agagggcgcg gatggggaaa
     4381 tgcccgaata tcttgaactt catggcgggc gggaagatga tatttccgca ccttcgcaac
     4441 ccgaaccgcc gacccgcaat atcaaaccgc tgcttttcgg gctgctgatt gcctttttgg
     4501 gctatctgct cggtaagatt ttttgattgt ccgataaatg ctgtattcgg gattttatat
     4561 atgaaatggt tgaaacgcct gacggtcatt gtcgggactt tttaccgcta tcggctggca
     4621 ggtctgtgtg cttcgctgat gggtagcggt tggatatgcg ctctgctgaa aatgatgccg
     4681 cagtcgtcca aattgaaaaa cgaaccgcct gccgtctgtc tgcgccttgc cttggaaagc
     4741 ctggggccga ttttcatcaa gttcgggcag gttttgtcca cacgccccga tttgattccg
     4801 cacgattacg ccgtcgaact ggcaaagctg caagacaaag tgccgccttt tgacgcgcgg
     4861 ctttcgcgtg aacaaatcga aaaatcgctg ggtcagccca tcgaaaagct gtatgcggaa
     4921 tttgaaaccg agcccatcgc cagcgcgtcc atcgcccaag tacacaaagc ccgcctgcat
     4981 tcgggcgaac aagtggcggt caaagtcttg cgccccaacc ttttgcccgt gatcgaacag
     5041 gatttgtcgc tgatgcgctt tggtgcaggc tgggtcgagc gtctgtttgc cgacggcaag
     5101 cgtctgaagc cgcgcgaagt ggtggcggag ttcgacaaat atctgcacga cgagttggac
     5161 ttgatgcgcg aagccgccaa tgccagccag ctcggacgca atttccaaaa cagcgatatg
     5221 ctgattgtgc cgaaggtgtt ttacgactac tgcaccagcg acgtgctgac catcgaatgg
     5281 atggacggca cgccggtttc cgacatcgcc aaactcaaag cagacggcat cgatttgcac
     5341 aaactcgccg attacggcgt ggaaatcttt ttcacgcagg tcttccgcga cggttttttc
     5401 cacgcggata tgcaccccgg caatattttg gttgccgccg acaaccgcta catcgccctc
     5461 gatttcggca tcgtcggcac gctgaccgat tacgataaac gctacctcgc catcaacttc
     5521 ctcgccttct tcaaccgcga ttaccggcgc gtcgccaccg cccacatcga atcgggctgg
     5581 gtgcctgccg acacgcgtgc ggaagaattg gaagccgccg tccgcgccgt gtgcgaaccg
     5641 gtgttcaaca aaccgatttc gcagatttcc ttcggcttgg tgctgatgcg cctgtttgaa
     5701 gtcagccgcc gcttcaatgt cgaaatccaa ccgcagcttg tgctgctgca aaaaacgctg
     5761 ctcaacatcg aaggcttggg ccgccagctc gatcccgatt tggacttgtg gaaaaccgcc
     5821 aaaccgtttt tggtgaaatg gatgaacggg caggtcggcc ctaaagccct ttggcgcaac
     5881 ctcaaaaacg aagcccccga ctgggcgcaa atcattcctt cattgccgcg caaaatcagt
     5941 gcgttgattg atgaaaaccg ccagcaggaa atgcgtgatg cctatatcca tttggtcaaa
     6001 gtgcagcagc ggcaaagcct gtggctggct gtgattgcgg ttgttttgct gctgattttg
     6061 cttttgaaat aggctttgtc cgaatcatcg cccgactccg cccgtttata aggaaatc
//
LOCUS       DAXHSF010000122         6024 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.122, whole genome shotgun sequence.
ACCESSION   DAXHSF010000122 DAXHSF010000000
VERSION     DAXHSF010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6024
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.122"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(57..1460)
                     /gene="mtrE"
                     /locus_tag="ACFM8S_001592"
     CDS             complement(57..1460)
                     /gene="mtrE"
                     /locus_tag="ACFM8S_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MtrE"
                     /protein_id="HFC6314645.1"
                     /translation="MDTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFKNDTAD
                     SGIRAVDLGWHDYFADPRLQKLIDIALERNTSLRTAALNSEIYRKQYMIERNNLLPTL
                     AANANDSRQGSLSGGNVSSSYKVGLGAASYELDLFGRVRSSSEAALQGYFASTANRDA
                     AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQE
                     ALIESAKADYAHAARSREQARNALATLINQPIPDDLPAGLPLDKQFFVEKLPAGLSSE
                     VLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGTVGTGSAELGGLFKSGTGVWS
                     FAPSITLPIFTWGTNKANLDVAKLRQQAQIVAYESAVQSAFQDVANALAAREQLDKAY
                     DALSKQSRASKEALRLVGLRYKHGVSGALDLLDAERSSYSAEGAALSAQLTRAENLAD
                     LYKALGGGLKRDTQTDK"
     gene            complement(1515..4718)
                     /gene="mtrD"
                     /locus_tag="ACFM8S_001593"
     CDS             complement(1515..4718)
                     /gene="mtrD"
                     /locus_tag="ACFM8S_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MtrD"
                     /protein_id="HFC6314646.1"
                     /translation="MAKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPTIT
                     LRATYPGASAQVMEDSVLSVIERNMNGVEGLDYLSTSADSSGSGSVSLTFTPDTDENL
                     AQVEVQNKLSEVLSTLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNI
                     VPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADIGSALSAQNVQISAGSIG
                     SLPAVRGQTVTATVTAQGQLGTAEEFGNVILRANTDGSNVYLKDVARVGLGMEDYSSS
                     TRLNGVNTTGMAVMLSNSGNAMATAKAVKERMATLEKYFPQGMSWKTPYDTSKFVEIS
                     IEKVIHTLIEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSINVLT
                     MFAMVLVIGIVVDDAIVVVENVERIMADEGLPPKEATKKAMGQISGAVIGITAVLISV
                     FVPLAMFSGATGNIYKQFALTMASSIAFSAFLALTLTPALCATMLKTIPKGHHEEKKG
                     FFGWFNKKFNSWTHGYEGRVAKVLRKTFRMMVVYIGLAVVGVFLFMRLPTSFLPTEDQ
                     GFVMVSVQLPAGATQERTNATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFA
                     ILKDWNERTAPGSDAVAIAGKLTGMMMGTLKDGFGIAVVPPPILELGNGSGLSINLQD
                     RNNTGHTALLAKRNELIQKMRASGLFDPSTVRAGGLEDSPQLKIDINRAAAAAQGISF
                     ADIRTALASALSSSYVSDFPNQGRLQRVMVQADEDARMQPADILNLTVPNKSGVAVPL
                     STIATVSWENGTEQSVRFNGYPSMKLSASPATGVSTGQAMAAVQKMVDELGGGYSFEW
                     GGQSREEAKGGSQTLILYGLAVAAVFLVLAALYESWSIPLAVILVIPLGLIGAAAGVT
                     GRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAAL
                     EAARLRFRPIIMTSFAFILGVVPLYIAGGASSASQRAIGTTVFWGMLIGTLLSVFLVP
                     LFYVVVRKFFKETAHEHEMAVKHAAEAGITGSDDSQH"
     gene            complement(4730..5968)
                     /gene="mtrC"
                     /locus_tag="ACFM8S_001594"
     CDS             complement(4730..5968)
                     /gene="mtrC"
                     /locus_tag="ACFM8S_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit MtrC"
                     /protein_id="HFC6314647.1"
                     /translation="MAFYAFKAMRAAALAAAVALVLSSCGKGGDAAQGGQPAGREAPA
                     PVVGVVTVHPQTVALTVELPGRLESLRTADVRAQVGGIIQKRLFQEGSYVRAGQPLYQ
                     IDSSTYEAGLESARAQLATAQATLAKADADLARYKPLVAAEAVSRQEYDAAVTAKRSA
                     EAGVKAAQAAIKSAGINLNRSRITAPISGFIGQSKVSEGTLLNAGDTTVLATIRQTNP
                     MYVNVTQSASEVMKLRRQIAEGKLLAADGVIAVGIKFDDGTVYPEKGRLLFADPTVNE
                     STGQITLRAAVPNDQNILMPGLYVRVLMDQVAADNAFVVPQQAVTRGAKDTVMIVNAQ
                     GGMEPREVTVAQQQGTNWIVTSGLKDGDKVVVEGISIAGITGAKKVTPKEWAPSENQA
                     AAPQSGVQTASEAKTASEAE"
BASE COUNT         1350 a         1705 c         1626 g         1343 t
ORIGIN      
        1 aaagaaatgc cgtctgaaac cttatcgttt cagacggcat tttattgccc gaccggttat
       61 ttgtcggttt gggtatcccg tttcaatccg ccgccgagtg ccttgtacaa atcggcaagg
      121 ttttcggcgc gggtcagttg tgccgacaaa gccgcacctt ccgccgaata gctgctgcgt
      181 tccgcatcga gcaagtcgag cgcgccggat acgccgtgct tgtaacgcag gccgaccaag
      241 cgcaacgctt ctttagaggc gcggctttgt ttgcttaaag cgtcataggc tttatccagc
      301 tgctcgcgcg ccgccaatgc gtttgccacg tcttgaaatg cggattggac ggcggattca
      361 taggcaacga tttgtgcctg ttggcgcagc ttggctacat caaggttcgc cttgttcgta
      421 ccccaggtaa aaatcggcag ggtaatagac ggcgcgaacg accaaacgcc cgtgccgctt
      481 ttgaacaacc cacccaattc ggcagaaccc gtaccgacgg ttccggtcag gcggatggat
      541 gggaaaaagg cggcgcgtgc cgcaccgata ttggcgtttg cctgtttgag cgcgtgttcg
      601 gcagcacgga tatcgggacg gtcgagcaat acttcggaac tcaaaccggc cggcagtttt
      661 tcaacaaaaa actgcttgtc cagcggcaaa ccggcgggca ggtcgtcggg tatcggttgg
      721 ttaatcaagg ttgccaaggc attgcgcgcc tgttcgcggc tgcgcgcggc atgggcataa
      781 tcggctttgg cagattcgat tagggcttcc tgctgacgta gggcgacggc ggaaatcacg
      841 cctgccttgt aacgtaattc ggacagcttg taggtttcct cgcgcgtttt caaaacacgt
      901 tgcgccaaag acatcgcttc ttcggcgtaa cgttcgttga aataggcttt ggcaacggtg
      961 gcaatcaggc tcaaatgtgc cgcatcgcgg ttggcggtgc tggcgaaata gccttgcagt
     1021 gccgcctcgc tgctgctgcg tacacgcccg aacagatcga gttcgtaaga tgccgcaccc
     1081 agtccgactt tgtagctgct gcttacattg ccgccgctca agctgccttg gcgcgagtcg
     1141 ttcgcattgg cggcaagcgt gggcaggagg ttgttgcgct caatcatgta ttgtttgcgg
     1201 tagatttcgc tgttcaatgc ggcggtacgc aaactggtat tgcgctcgag tgcgatgtcg
     1261 atcagctttt gcaggcgcgg gtcggcaaaa tagtcatgcc aacctaaatc gaccgcacgg
     1321 atgccgctgt cggcggtatc gtttttgaac gtttcggcaa cttcgacttt gggctgctcg
     1381 tattggggaa tcatggtgca ggcggataat gcgaaggctg ctgcaacaga agtcaaggtg
     1441 gttttcaatg tagtatccat aaaaaagtcc tgatgccgtc tgaaaacccg tgggcgttca
     1501 gacggcatgg ttgcttaatg ttggctgtcg tccgaaccgg tgatgcccgc ttcggcagcg
     1561 tgttttactg ccatttcgtg ttcgtgtgca gtttctttga agaatttgcg taccaccaca
     1621 tagaaaagcg gaacaaggaa cacggacaag agcgtgccga tgagcatccc ccagaatacg
     1681 gttgtaccaa tggcgcgctg gctggcagaa cttgcaccgc cggcaatata caggggaacc
     1741 acgcccaaaa taaaggcgaa cgaggtcatg ataatcggac ggaaacgcag gcgggctgct
     1801 tccaaagcgg cttcaaccgc gcttttccct tgcgcttgaa ggtctttggc aaactcgata
     1861 atcaaaatcg cgtttttcgc actcaaaccc atcacggtaa cgaaaccgac ttgaaagtag
     1921 atgtcgttgg cgaacgaggg aacgctgccc aacagtcctt caaacaggtt gcgcccggtt
     1981 acgcccgcag ccgcaccgat caaacccaac ggaatcacaa ggatgaccgc cagcggaatc
     2041 gaccagcttt cataaagcgc ggcaagtacc aaaaatacgg ctgcaaccgc caaaccgtac
     2101 aaaatcaggg tttgcgagcc gccttttgcc tcttcgcgcg actgtccgcc ccattcgaag
     2161 ctgtaaccgc cgcccaattc gtcaaccatt ttttgaaccg ccgccatagc ctgcccggtg
     2221 gaaacgccgg ttgcaggcga agcggacagc ttcatcgaag gataaccgtt gaagcgtacg
     2281 ctctgttccg taccgttttc ccaagaaaca gtagcaatgg tggaaagcgg tacggcgacg
     2341 ccggatttgt tcggcacggt caggttcaaa atatcggcag gctgcatacg ggcatcctcg
     2401 tcggcctgca ccatcacgcg ttgcagacgg ccttggttcg ggaagtcgct gacataagac
     2461 gaactcagcg cgcttgccaa tgcggtgcgg atgtcggcaa acgaaatgcc ttgcgccgcc
     2521 gccgcggcac ggttgatgtc gattttcaac tgcggcgagt cttccaaacc gccggcacgg
     2581 acggtgctgg ggtcaaacaa accgctggca cgcattttct gaatcaactc gttgcgcttc
     2641 gccagcaatg cggtatggcc ggtattattg cggtcttgca ggttgatgct cagacccgaa
     2701 ccgttgccca actccagaat cggaggcggg acgacggcga tgccaaaacc gtctttaagc
     2761 gtccccatca tcatacccgt cagcttgccg gcaatcgcaa cggcatcgct gccgggcgcg
     2821 gtacgctcgt tccaatcttt caatatggca aaacccatcg ccatattctg accgctgccc
     2881 gaaaagctga agccggaaac ggtaatgatg ttttctattt caggaatgct tttcgccagt
     2941 tgggtaactt gcgccaaagt cgcattggtg cgctcttggg tcgctcctgc aggcagttgc
     3001 acgctgacca tgacgaagcc ttggtcttcg gtcggcagga atgaagtcgg caggcgcata
     3061 aacaggaaca cgcccacaac cgccaagccg atatagacaa ccatcatgcg gaaagtctta
     3121 cgcagcactt tggcaacccg gccttcgtaa ccgtgcgtcc aactattgaa tttcttgtta
     3181 aaccagccga agaaaccttt tttctcttcg tgatgccctt tcgggattgt cttcaacatt
     3241 gtggcacaca aagcaggggt aagggtcagc gcaaggaagg cggagaatgc gattgatgac
     3301 gccatcgtca gggcaaactg tttgtaaata ttgcccgtcg ccccgctgaa catcgccaac
     3361 ggtacgaaca cggaaatcag aacggcggta ataccgatga ccgcacccga aatctgaccc
     3421 atcgcttttt tggtcgcttc tttgggcggc aagccttcat ccgccataat gcgctcgacg
     3481 ttttcaacca ccacaatcgc gtcatcgacc acgatgccga tgaccaaaac catcgcaaac
     3541 atggtcagta cgttaatcga catgcccata taagagatga aggcgaaacc gcccaacagc
     3601 gaaatcggta cgacgatggt cggaatcagc gtataacgga tgttttgcag gaagagatac
     3661 attacgacaa acaccaacac catcgcttcg attaaagtgt gaatcacttt ttcaatcgaa
     3721 atttcgacga atttggaagt atcgtaaggg gttttccagc tcataccctg aggaaagtat
     3781 ttttccaacg tcgccatgcg ttctttaacc gcctttgccg tcgccatcgc attgccgctg
     3841 ttggacagca tcaccgccat accggtggta tttacaccgt tcagacgggt tgaggaagaa
     3901 tagtcttcca tacccagtcc gacccttgcc acatccttca ggtaaacatt agaaccgtcg
     3961 gtattggcgc ggagaatgac gttgccgaat tcttcggccg tacccaactg cccttgcgcc
     4021 gttacggtag ccgtaaccgt ctgtccgcga acggcgggaa gcgaaccgat agaacccgct
     4081 gaaatctgga cgttctgggc ggacagcgca ctgccgatat cggcaaacga caaattgtag
     4141 ttttgcagtt tcttaggatc aacccaaatc cgcatcgcgc gttgcgcgcc gaacaggcgt
     4201 acctgcccca cgccttcgat acgctgcagt tcgggaacga tattacgctg cgcgtagtcg
     4261 ttcatctctt cggttgactg cacatccgac gaaagcatca caatcatcag gaaattggaa
     4321 cgcgccttgg atacggttac gccgtattgc tggacggttg ccggcagcgt gctcaatact
     4381 tcggaaagct tgttctgcac ttccacctgc gccagattct cgtcggtatc gggcgtaaag
     4441 gtcaggctca cgctgccgct gccgctcgaa tcggcggaag tggacagata atccaaacct
     4501 tccacgccgt tcatattccg ctcgatcacg gaaagcacgc tgtcttccat tacctgcgcg
     4561 gacgcgcccg gataagtggc cctcagggtg atggtcgggg cggcgacaga cggatattgc
     4621 gaaacaggca ggcttttaat gccgaaaata cccgccgcaa taatgaaaat cgaaataacc
     4681 cacgcgaaaa tggggcggtc gataaaaaat ttagccatcg atgccttcct tattccgctt
     4741 cagaagcagt tttggcttca gatgccgtct gaacgccgga ttgaggcgcg gcggcttggt
     4801 tttcagacgg cgcccattct ttgggcgtta cctttttcgc acccgttata ccggcgatac
     4861 tgatgccttc cacaaccacc ttgtccccgt ccttcagacc cgacgtaaca atccaattcg
     4921 taccctgctg ttgcgcgacc gttacctcgc ggggttccat accgccttgg gcattcacaa
     4981 tcatcacggt atctttcgca ccgcgcgtta ctgcctgctg cggaacgacg aacgcattat
     5041 ccgccgccac ctgatccatc agcacgcgca catacaggcc gggcatcagg atattctgat
     5101 cgttcggtac ggcggcgcgc agggtaatct gaccggtcga ttcgttgacg gtcggatcgg
     5161 caaacagcag gcggcctttt tcagggtaaa ctgtgccgtc gtcaaatttg atgccgaccg
     5221 caatcacacc atccgccgcc agcagtttgc cttcggctat ctgacggcgc aatttcatca
     5281 cttcggatgc agactgggta acgttcacat acatcggatt ggtttggcgg atggttgcca
     5341 gcacggtcgt atcgcccgca ttcaacagcg taccttcgga aactttggac tgaccgataa
     5401 agccggaaat cggcgcggta atgcgcgaac ggttcagatt gatgccggca gatttgatcg
     5461 ccgcctgtgc tgctttgaca cctgcctcgg cagaacgttt cgccgttacc gcagcatcgt
     5521 attcctgccg gctgacggct tcggcggcaa ccaaaggctt gtaccgcgcc aaatccgcat
     5581 ccgctttggc aagcgttgcc tgagccgttg ccagttgcgc gcgcgcactt tccagacctg
     5641 cttcataagt ggaactgtcg atctgataca gcggctgtcc ggcacggaca taactgcctt
     5701 cttggaacag gcgtttttgg atgatgccgc cgacttgggc gcggacatcg gcggtacgca
     5761 gcgattccaa acgccccggc aactcgacgg tcaatgcgac ggtttgcgga tggacggtta
     5821 cgacaccgac gacgggcgca ggggcttccc gaccagcagg ctgcccgccc tgcgccgcgt
     5881 ctccgccttt accgcaagac gacagtacca atgcaacggc ggcagccaac gcggccgcac
     5941 gcatcgcctt aaaagcataa aaagccatta tttatcctat ctgtctggtt tgatgtaaag
     6001 ggttttgcca atcaacaggc attc
//
LOCUS       DAXHSF010000123         6014 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.123, whole genome shotgun sequence.
ACCESSION   DAXHSF010000123 DAXHSF010000000
VERSION     DAXHSF010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6014
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.123"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(99..2324)
                     /locus_tag="ACFM8S_001595"
     CDS             complement(99..2324)
                     /locus_tag="ACFM8S_001595"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFC6314648.1"
                     /translation="MTQKSTIVYTHTDEAPALATQSLLPIVQAFTRHADIDVKTADIS
                     LSGRILAAFPEYLTEAQRVPDALAELGELVKQPDANVIKLPNISASVPQLTAAIKELQ
                     SKGFAVPDYPADPQTDEEKAVRERYDRIKGSAVNPVLREGNSDRRAPKAVKNFAKKHP
                     HSMGAWTKDSKTHVATMQSGDFFHNEQSVTVPEATSVSIVFTDKQGNKKELRAPVALK
                     AGEIIDATVMSKKALLAFLAEQVKDAKAKGVLFSLHMKATMMKVSDPIIFGHAVKVFF
                     APVFEKFGDKLAAAGVNVNNGFGNLLANLDKLDADTRAAVEAEIAAVYAANPDLAMVD
                     SDKGITNLHVPSDVIVDASMPAMIRNSGRMWDKDGKAQDTKAVIPDSSYAGVYQATID
                     FCREHGAFDPTTMGTVPNVGLMAQAAEEYGSHNKTFEIEADGQVQVIDAEGKVLMQHD
                     VEAGDIWRMCQTKDAPVKDWVQLAVNRARLSNTPAVFWLDENRPHDKSLLAKVKAYLA
                     ELDTNGLDIRVLAPEEAAKFSLGRLKNGEDTISVTGNVLRDYLTDLFPILELGTSAKM
                     LSIVPLMNGGGMFETGAGGSAPKHVQQFLEENHLRWDSLGEFLALAVSFEHLAQKTGN
                     AKAQVLADTLDAATEKLLLNDKSPKRKAGELDNRGSHFYLTLYWAQELAEQDKDAELK
                     AAFTPLAAALTADEAKIVAELSAVQGKAVDIGGYYAANPEKAAQAMRPSVTFNQALNA
                     L"
     gene            2759..3874
                     /gene="lst"
                     /locus_tag="ACFM8S_001596"
     CDS             2759..3874
                     /gene="lst"
                     /locus_tag="ACFM8S_001596"
                     /EC_number="2.4.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyllactosaminide
                     alpha-2,3-sialyltransferase"
                     /protein_id="HFC6314649.1"
                     /translation="MGLKKACLTVLCLIVFCFGIFYTFDRVNQGERNAVSLLKEKLFN
                     EEGEPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFNQIKDKAER
                     AYFFYLPYGLNKSFDFIPTMAELKVKSMLLPKVKRIYLASLEKVSIAAFLSTYPDAEI
                     KTFDDGTGNLIQSSSYLGDEFSVNGTIKRNFARMMIGDWSIAKTRNASDEHYTIFKGL
                     KNIMDDGRRKMTYLPLFDASELKAGDETGGTVRILLGSPDKEMKEISEKAAKNFNIQY
                     VAPHPRQTYGLSGVTTLNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNV
                     HVYALKPASLPEDYWLKPVYALFTQSGIPILTFDDKD"
     gene            complement(4038..4496)
                     /locus_tag="ACFM8S_001597"
     CDS             complement(4038..4496)
                     /locus_tag="ACFM8S_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFC6314650.1"
                     /translation="MKTQISLAAAAITLLLSACGGSGIPSQPKGEISENRTAAFKSMM
                     PDFSRMGKMVKGEEPYDVEKFKQAAASFADSSKKPFTLFESDPQGNGRALPAVWSDGA
                     KFEAEKTKFAAAVEKLNAAAQTGKLDEIKAAYGETGASCKSCHDSFRAPE"
     gene            4615..5436
                     /locus_tag="ACFM8S_001598"
     CDS             4615..5436
                     /locus_tag="ACFM8S_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC6314651.1"
                     /translation="MAVLITGASAGFGEAMCRAFVGAGYRVIGAARRADRLQTLADEL
                     GALFYPLEMDVSRRESVENALNGIPDEFSDIDCLINNAGLALGLDTADKADFEDWETM
                     IQTNVLGLTFLTRKILPNMVARGGGYVMNLGSIAGNYAYAGSNVYGATKAFVRQFSLN
                     LRAELADKNIRVTNIEPGLCGNTEFSNVRFKGDDEKAAGVYEGVEFIRPEDIAETALW
                     LYRRPAHMNVNTIEIMPVAQTFAGMKVIKKAVPEVREDFEKQSMSLFSRIRSWFK"
     gene            complement(5487..5933)
                     /gene="yciA"
                     /locus_tag="ACFM8S_001599"
     CDS             complement(5487..5933)
                     /gene="yciA"
                     /locus_tag="ACFM8S_001599"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFC6314652.1"
                     /translation="MQHEEGNRQRPQGELLLRTVAMPRDTNPNQDIFGGWIMSQMDLG
                     GGILAAEIARGRIVTVAVQEMNFIRPVKVGNVVCCYGHCVRVGNTSLQLKIEVWVKTL
                     MNDCVTEDRYLVTEAVFTYVAIDAEGNPRPIPKEGNPKLSGLLPTP"
BASE COUNT         1316 a         1332 c         1744 g         1622 t
ORIGIN      
        1 atatccaatg ggtgcggcgt ttaatcataa tcaggcagat agggataact aatgccgtct
       61 gaacgacgaa tgttcagacg gcatttttac ctttgtgctt ataaggcgtt tagtgcctga
      121 ttaaaggtta cgctcgggcg catcgcttgt gccgcttttt caggattggc tgcgtagtag
      181 ccgccgatgt cgaccgcttt gccttgtacg gcggaaagct cggcaacgat tttcgcttcg
      241 tcggcggtca aagcggctgc caatggcgta aatgcggctt tcagttcggc atctttgtct
      301 tgctccgcca attcttgcgc ccagtagagg gtaaggtaga aatggctgcc ccggttgtcg
      361 agttcgccgg ctttacgttt aggcgatttg tcgttcaaca gcagtttttc ggtggctgca
      421 tccaaagtgt cggcgaggac ttgggctttg gcattgccgg ttttttgcgc caaatgttca
      481 aacgatacgg cgagtgcgag gaattcgccc agcgagtccc agcgcaaatg gttttcttcg
      541 aggaactgtt gaacatgttt cggtgcagaa ccgcccgcgc cggtttcaaa cataccgccg
      601 ccgttcatca atggaacgat agacagcatt ttcgcgcttg tgccgagttc caaaattggg
      661 aacaagtcgg tcaggtagtc gcgcaagaca ttaccggtta cggagatggt gtcttcgccg
      721 tttttcagac gacccaagct gaacttggcg gcttcttcag gagcgaggac gcggatgtcg
      781 aggccattgg tatccagttc ggcaaggtag gctttaacct tggcgagcag gctcttgtcg
      841 tgcggacggt tttcatcgag ccagaacacg gcaggcgtgt tgctcaggcg ggcgcggttg
      901 acggcgagtt gcacccagtc tttgaccgga gcgtctttgg tttggcacat acgccagatg
      961 tcgcctgctt caacgtcgtg ctgcatcagg acttttcctt ccgcatcaat gacttgaacc
     1021 tgaccgtcgg cttcgatttc aaaggttttg ttgtgcgagc cgtattcttc cgccgcttgc
     1081 gccatcagtc cgacgttggg cacagtaccc atggttgtcg ggtcaaatgc gccgtgttcg
     1141 cggcagaagt cgatggttgc ttggtaaacg ccggcatagc tgctgtcggg aatcacggct
     1201 ttggtgtctt gtgctttgcc gtctttatcc cacatacggc cggaattacg aatcatcgca
     1261 ggcatagaag catcgacgat gacatcgcta ggaacgtgca ggttggtgat gcctttgtcg
     1321 gaatcaacca tggctaaatc agggttggcg gcgtaaacgg cggcgatttc agcttcaacg
     1381 gcagcgcggg tgtccgcatc cagtttgtcc agattggcaa gcaggttgcc gaagccgttg
     1441 ttaacgttga cgccggcagc agccagtttg tcgccgaatt tttcaaatac aggcgcgaag
     1501 aatactttga cggcatgtcc gaagataatc gggtcggaca ctttcatcat ggtggctttc
     1561 atgtgcagcg agaacaatac accttttgct ttcgcgtctt tgacttgttc ggcaaggaag
     1621 gcgagcaggg cttttttgct catcacggtt gcgtcgatga tttcgccggc tttcagggca
     1681 acaggcgcgc gcagctcttt tttattgcct tgtttgtcgg tgaatacgat ggatacggaa
     1741 gtcgcttcag gtacggtaac ggattgttcg ttatggaaaa aatcgccgct ttgcatggtg
     1801 gcgacgtggg ttttggagtc tttggtccat gcgcccatgc tgtgcggatg ttttttcgca
     1861 aagtttttca ctgctttagg cgcgcgacgg tcggagttgc cttcacgcag gacagggttt
     1921 accgcgctgc ctttgatgcg gtcgtagcgt tcgcgcacgg ctttttcttc atcggtttgc
     1981 gggtcggcag gataatcggg aacggcaaag cctttagact gcaattcttt aatcgcggcg
     2041 gtcagttgcg gtacggatgc gctgatgttc ggcagtttga ttacgtttgc atcgggttgt
     2101 tttaccagtt cgcccaattc ggcaagcgcg tcgggtacgc gttgcgcttc ggtcagatat
     2161 tcggggaatg ccgccaaaat acggccggac agggaaatgt cggcagtttt gacatcaata
     2221 tcggcgtggc gggtaaaagc ctgcacaatc ggcagcagcg attgggtcgc cagcgcgggg
     2281 gcttcgtcgg tatgggtata aacaatggtg gatttttgag tcataggatt attctcttgt
     2341 aggttggttt tttcttttgg aacacattgc gcggggaatg tgcgcggcta ttatggcata
     2401 ttttggcggc tttgttcgcg ctttgttcga tcttggcgtg tttgaacgcg gcggcgtgaa
     2461 aggaagggag aaatggtttt cccgcgtttg gtggcggtgt cggaggtgct gtgcctgatg
     2521 tgcggcggca tattttcggt gaaattgatt ttatagtggt ttaaatttaa accagtacag
     2581 cgttgccttg ccttgtcgta ctgtttgtac tgtctgcggc ttcgtcgcct tgtcctgatt
     2641 taaatttaaa ccactataat attcggtaac tgtcggaata tctgctaaaa ttccgcattt
     2701 ttccgtcccg ggacactcgg ggcgtatgtt caatttgtcg gaatggagtt ttagggatat
     2761 gggcttgaaa aaggcttgtt tgaccgtgtt gtgcctgatt gttttttgtt tcgggatatt
     2821 ttatacattt gaccgggtaa atcaggggga aaggaatgcg gtttccctgc tgaaggagaa
     2881 acttttcaat gaagaggggg aaccggtcaa tctgattttc tgctatacca tattgcagat
     2941 gaaggtggca gaaaggatta tggcgcagca tccgggggag cggttttatg tggtgctgat
     3001 gtctgaaaac aggaatgaaa aatacgatta ttatttcaat cagataaagg ataaggcgga
     3061 gcgggcgtat tttttctacc tgccctacgg tttgaacaaa tcgtttgatt tcattccgac
     3121 gatggcggag ctgaaggtga agtcgatgct gctgccgaag gtcaaacgga tttatttggc
     3181 aagtttggaa aaagtcagca ttgccgcctt tttgagcact tacccggatg cggaaatcaa
     3241 aacctttgac gatgggacag gcaatttaat tcaaagcagc agctatttgg gcgatgagtt
     3301 ttctgtaaac gggacgatca agcggaattt tgcccggatg atgatcggag attggagcat
     3361 cgccaaaacc cgtaatgctt ccgacgagca ttacacgata ttcaagggtt tgaaaaacat
     3421 tatggacgac ggccgccgca agatgactta cctgccgctg ttcgatgcgt ccgaactgaa
     3481 ggcgggggac gaaacgggcg gcacggtgcg gatacttttg ggttcgcccg acaaggagat
     3541 gaaggaaatt tcggaaaagg cggcaaaaaa cttcaacata caatatgtcg caccgcaccc
     3601 ccgccaaacc tacgggcttt ccggcgtaac cacattaaat tcgccctatg tcatcgaaga
     3661 ctatattttg cgcgagatta agaaaaaccc gcatacgagg tatgaaattt ataccttttt
     3721 cagcggcgcg gcgttgacga tgaaggattt tcccaatgtg cacgtttacg cattgaaacc
     3781 agcttccctt ccggaagatt attggctcaa gccggtgtat gccctgttta cccaatccgg
     3841 catcccgatt ttgacatttg acgataaaga ttaatcgcat agcaaatcaa aatagaaaat
     3901 ggcggagtaa gtaaggcaaa aatcaggata tggcgtattt tttgaattga agataatttc
     3961 tgattgcttt gcgtgtgctg aaatgacaaa gaaaatgccg tctgaaggct tcagacggca
     4021 tcgttctgtt tcggatgtta ttccggcgcg cggaaactgt cgtggcagga tttgcagctt
     4081 gcgccggttt cgccgtaggc ggctttgatt tcatccagtt tgccggtttg tgctgcggcg
     4141 ttgagttttt cgacggcggc ggcgaatttt gttttttcgg cttcaaattt tgcaccatcc
     4201 gaccaaacgg ctggcagggc gcggccgtta ccttgcgggt cggactcaaa aagcgtaaac
     4261 ggttttttgc tgctgtcggc aaacgacgct gccgcctgtt tgaatttttc gacatcgtaa
     4321 ggctcttcgc ctttgaccat tttgcccata cgcgagaagt cgggcatcat agatttgaac
     4381 gcggcggtgc ggttttcgga aatttcacct ttgggttggg aaggtattcc gctgcctccg
     4441 caggcggaaa ggagcagtgt gatggcggca gcagcaaggc tgatttgggt tttcatattg
     4501 aatgtgtcct gtcgtggtgg tatggttgtt gtcattttca gccggcgcga tgccgtctga
     4561 agagcgttat gatacctgaa acaggtttgg gaaacgagaa agaaaggaaa aacaatggct
     4621 gttttaatta ccggtgcttc ggcaggattc ggcgaagcga tgtgccgcgc gtttgtcggg
     4681 gcgggatacc gcgttatcgg tgcggcgcgc cgtgcggaca ggcttcagac cttggcggat
     4741 gaattgggtg ctttgtttta ccctttggaa atggacgtgt cgcgacgcga gtcggtggaa
     4801 aacgccttaa acggcatccc cgatgaattt tccgacatcg actgcctcat caacaatgcc
     4861 gggctggctt tgggtttgga cacggcggac aaggcggatt ttgaagattg ggaaacaatg
     4921 attcaaacca atgttttggg tttgacgttt ctaacgcgca agattttacc gaatatggtg
     4981 gctcggggcg gcggttatgt gatgaatttg ggttcgattg caggtaatta tgcttatgcc
     5041 ggcagcaacg tttacggggc gaccaaggcg tttgtgcgcc agtttagcct gaatttgcgc
     5101 gcagagttgg cggataagaa catccgcgtt accaatatcg agccgggttt gtgcggcaat
     5161 acggagtttt ccaacgtgcg cttcaaaggc gatgacgaga aggcagcagg cgtgtatgag
     5221 ggtgtggaat ttatccgccc cgaagatatt gcggaaaccg cattgtggct gtaccgccgg
     5281 ccggcgcata tgaatgtgaa cacgattgaa attatgcccg tggcgcagac ttttgcagga
     5341 atgaaagtga taaaaaaagc cgtgccggaa gtgcgggaag actttgaaaa acagagtatg
     5401 tcgctgtttt cccgcatcag gtcctggttc aaatgatgcg gaatgccgtc tgaagacagt
     5461 ttcagacggc atttttacgg gtatttttac ggagtaggca ataagcccga caatttgggg
     5521 ttgccttctt tcggaatcgg gcgcggattg ccttccgcat cgatggcaac ataagtgaac
     5581 accgcctcgg ttacgaggta gcggtcttcg gtaacgcaat cgttcatcaa agttttcacc
     5641 cagacttcga ttttaagctg gagggaagta ttgcccacgc ggacgcaatg cccgtagcag
     5701 cagacgacgt tgccgacctt gaccgggcgg ataaagttca tttcctgaac ggcgacggta
     5761 acgatgcgtc cccgcgcgat ttccgccgcc aatatgccgc cgcccaaatc catctgcgac
     5821 ataatccagc cgccgaaaat gtcttggttg ggattggtat cgcgcggcat agcgacggta
     5881 cgcaggagca gttcgccttg agggcgttgg cggttgcctt cttcgtgctg cataaagttt
     5941 ccttgtttta ttgaaatata tagtggatta aatttaaacc agtacggcgt tacctcgcct
     6001 tagctcaaag agaa
//
LOCUS       DAXHSF010000124         6008 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.124, whole genome shotgun sequence.
ACCESSION   DAXHSF010000124 DAXHSF010000000
VERSION     DAXHSF010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6008
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.124"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(53..361)
                     /locus_tag="ACFM8S_001600"
     CDS             complement(53..361)
                     /locus_tag="ACFM8S_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314653.1"
                     /translation="MTNNVDLKVPAKRYFTLDELCGLLQISPYGFAQWQHNHGVVVGY
                     GGERYTRLDVVKLLKLQSTFAPYAEGAESGSDGNRPVTLQEIGDGLKDLLADLDKELC
                     "
     gene            complement(345..647)
                     /locus_tag="ACFM8S_001601"
     CDS             complement(345..647)
                     /locus_tag="ACFM8S_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFC6314654.1"
                     /translation="MTLTKAELADILVDKVSNVTKNDAKEIVELFFEEIRSTLASGEE
                     IKISGFGNFQLRDKPQRPGRNPKTGEEVPITARRVVTFHASQKLKSMVEHYYDKQR"
     gene            complement(721..3084)
                     /gene="pheT"
                     /locus_tag="ACFM8S_001602"
     CDS             complement(721..3084)
                     /gene="pheT"
                     /locus_tag="ACFM8S_001602"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFC6314655.1"
                     /translation="MQFSYSWLKTQADTELSADKLEHLLTMSGLEVEEAETAAPAFAG
                     VVIAEVKSVEKHPDADRLNVTRVDAGTGGLVQIVCGAPNVKAGIKVPCSLPGAVLPGN
                     FKIKPTKMRGVVSNGMLCSTDELGLPDDGVNGLHILPEDAPVGTNIREYLDLDDTLFT
                     LKITPNRADCLSVKGIAREMSALTGCAFRQPEIHTAPITGSRKQPVQINAPADCGRFI
                     SRVIENVNAKAATPDWMKQRLERSGIRSISALVDIGNYVMLEIGQPMHVFDADKLSGS
                     LHIRRAREGETLECLNEKTVSLSENTLVVADEKGVLSLAGLMGGAASAVSDGTQNIVL
                     EAAWFAPEIIAGKSRQYGFGSDSSFRFERGVDYRLQADAIERATELVLQICGGAAGEM
                     VEAQGELPEAKQVGLRLGRLKTVLGVDIPAEQVETILQHLGLQPEKTAEGFRVTAPSF
                     RFDIEIEADLIEEIGRVYGYENIPDDYTSGRLKMLELPETRRPRFAVYNEMAARGYRE
                     VVSYAFVDEQWEQDFAANADPIRLQNPLAAQYAVMRSTLIGGLVEILQNNLNRKQNRV
                     RVFEIARVFGKGSDGQFVQNERIGGLWYGAAMPEQWGGKTRNADFYDIKADVENLLKN
                     KAVEFVKTGHPALHPGRAANIVSDGKVIGFVGELHPKWLQKYDLSQAPLVFEIDMAAV
                     LECGKTRYRAVSKFQPVRRDLAFVMPEAMSHDDLLLVLKGAANKLVQEISVFDVYRGT
                     GLPEGMKSVAVKVILQDMENTLTDEAVEPLIGKLIGAATAAGARLRS"
     gene            complement(3120..3638)
                     /locus_tag="ACFM8S_001603"
     CDS             complement(3120..3638)
                     /locus_tag="ACFM8S_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314656.1"
                     /translation="MSEIINLTESGITFNLVKNNLFYIEPYIRKRRQEGIKTVEFIYL
                     QEDNLLFVEAKQSIPNQERSPERFDEYISEIYQKWCNALNVEILGILGREDIKETIMP
                     SAFSNLQWGSIEIKLLLVIPDVPLNYLGQLNELIKQEFNKKDTLRLISLWNISVEVIN
                     RDLAIQKGLASS"
     gene            complement(3645..4709)
                     /locus_tag="ACFM8S_001604"
     CDS             complement(3645..4709)
                     /locus_tag="ACFM8S_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFC6314657.1"
                     /translation="MIKSFELEKFGPISLLKADNLGKINLIIAENSKGKTFILKALYS
                     VLKSHEEAHKGKNIRDFSEELRDKLYWTFQVEEIGDLVTRGKENPNERPLKLSMTLED
                     SSSVLFSFGRTTKKLIKPELYELSPRINANSIFLPPKEVLSLFDVIKKSEEEKRFGFD
                     ATYIDLVKALDIKPTKGRNYPEAAEARKDLEALFGGYVSYNDKKKAWVYEKDRQTFSI
                     NSTAEGVKKIGILDTLLGNRFLSPGSILFIDEPEANLHPKAINQFLEILFSLSKSKLG
                     IQIFMSTHSYFVIKKLCLLARKHSESIPVFMPGKEKEQTTWVQENLQNSMPDNEIIGE
                     SIRLFREEIGVLSPETAWIG"
     gene            complement(4886..5878)
                     /gene="pheS"
                     /locus_tag="ACFM8S_001605"
     CDS             complement(4886..5878)
                     /gene="pheS"
                     /locus_tag="ACFM8S_001605"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFC6314658.1"
                     /translation="MENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKT
                     LGQMSPEERKTIGAHINECKNRFQTAFNAKRDALNEAKLQARLAAEALDITLPGRAQE
                     GGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTFYV
                     ENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
                     VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSAEIDIMGENGKWLEVGGCGM
                     VHPNVLKNVNIDSEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFVK"
BASE COUNT         1520 a         1542 c         1268 g         1678 t
ORIGIN      
        1 attttttagc gtgtaaaaca aaaccggctg catacctgca accggcctca aatcagcaca
       61 attccttatc caaatccgcc aacaggtctt tcagaccgtc tccgatttcc tgaagcgtaa
      121 ccggacggtt gccgtccgaa cccgattccg caccttctgc atacggtgca aacgtgctct
      181 gcaatttcaa cagtttcacc acatccaaac gggtgtagcg ttcgccgccg taaccgacaa
      241 ccacaccgtg attatgctgc cattgcgcaa aaccataggg gctgatttgc aacagtccgc
      301 acaactcgtc cagcgtgaaa tagcgttttg cgggaacctt caaatcaacg ttgtttgtca
      361 tagtagtgtt ccaccatgct tttgagtttc tggctggcat ggaaagttac cacgcggcgg
      421 gcggtaatcg gcacttcctc gccggtttta ggattacgac ccgggcgttg cggcttgtcg
      481 cgcaactgga aatttccgaa accggaaatt ttgatttctt cgccgcttgc caaagtgctg
      541 cggatttctt caaaaaagag ttcgacgatt tctttggcat cgtttttggt gacgttgctg
      601 actttgtcta ccaaaatatc ggccagttct gctttagtga gagtcatatg attaccttct
      661 gttgtaacag ttgtggatta ttaccaattt ttatttaaaa ttcaagcgaa tatttatttt
      721 ttaactgcga agccgcgccc ctgccgccgt tgccgcgcca atcagttttc cgataagcgg
      781 ctcgactgcc tcatccgtca gcgtgttttc catatcctgc aaaatcactt tgaccgccac
      841 gctcttcatc ccttcgggca aacccgtgcc gcgatacacg tcaaacacgc tgatttcctg
      901 taccaacttg tttgccgcgc ctttcaagac aagcagcaaa tcatcatggc tcatagcttc
      961 cggcatcaca aacgccaaat cgcggcgcac cggctggaat ttcgatacgg cccgatagcg
     1021 cgttttcccg cattccaaca cggccgccat atcgatttca aataccagcg gcgcttgcga
     1081 caggtcgtat ttttgcagcc atttcggatg cagttcgccg acaaagccga tgactttgcc
     1141 gtctgaaacg atattggcgg cacgtccggg atgcagggcg ggatgtccgg ttttaacgaa
     1201 ctcgactgct ttgtttttca acagattttc cacgtccgcc ttgatgtcgt aaaaatctgc
     1261 attgcgcgtt ttcccgcccc attgttcggg cattgccgcg ccgtaccaca agccgccgat
     1321 gcgttcgttt tggacaaact ggccgtctga acctttgccg aacacgcggg cgatttcaaa
     1381 cacgcgcacg cggttttgtt tgcggttcag gttgttttgc aaaatttcca ccaagccgcc
     1441 gatgagtgtg gaacgcatca cggcatattg cgccgccaac gggttttgca ggcggatggg
     1501 gtcggcgttg gcggcaaaat cttgttccca ctgctcgtca acgaaggcat agctgaccac
     1561 ttcgcggtag ccgcgcgccg ccatttcgtt gtaaacggca aaacgcgggc ggcgtgtttc
     1621 gggcagttcc agcattttca gacggcctga cgtgtaatcg tcggggatgt tttcatagcc
     1681 gtaaacgcgt ccgatttctt caatcaaatc agcctcaatt tcgatgtcaa aacggaagct
     1741 cggcgcggta acgcggaagc cttccgccgt tttctcgggc tgcaggccca agtgttgcaa
     1801 aatggtttcc acctgttcgg caggaatgtc cacgcccaac acggttttca gacggcccaa
     1861 acgcaatccg acctgcttcg cttcgggcaa ttcgccttgc gcttccacca tttcgcctgc
     1921 cgcaccgccg caaatctgca acaccaattc ggtagcacgt tcaatggcat ccgcctgcaa
     1981 acggtaatcc acgccgcgct cgaagcggaa cgacgaatcc gaaccgaaac cgtattggcg
     2041 cgatttgccg gcgatgattt cgggcgcaaa ccaagccgct tccagcacga tattttgcgt
     2101 gccgtctgaa accgcgctcg ccgcgccgcc cattaagccc gccaaactca acacgccttt
     2161 ttcgtccgcc acgaccagcg tgttttcaga cagggaaacg gttttctcgt tcaggcattc
     2221 cagcgtttcc ccttcgcgcg cgcggcggat gtgcaggctg ccggaaagtt tgtcggcatc
     2281 aaaaacgtgc atcggctgac cgatttccag catcacataa ttgccgatgt ccaccagcgc
     2341 ggaaatactg cggatgccgc tgcgctccaa acgttgtttc atccaatcgg gagtagccgc
     2401 tttcgcattg acattttcaa taacgcggct gataaaacgg ccgcaatcgg caggcgcgtt
     2461 aatctgcacg ggctgttttc gactgcccgt gatcggcgcg gtatggattt cgggctgcct
     2521 gaacgcgcac cccgtcaatg cggacatttc gcgcgcaatg cccttaacac tcaagcaatc
     2581 agcgcggtta ggcgtaattt tcaacgtaaa cagcgtatcg tccaaatcca agtattcacg
     2641 gatattggta ccgacgggcg cgtcttcagg cagaatgtgc aggccgttca caccgtcgtc
     2701 gggcagaccg agttcgtcgg tggaacacaa catcccgttc gacaccacac cgcgcatctt
     2761 ggtcggcttg attttgaaat tgcccggcaa aacggcgccc ggcaacgaac acggcacttt
     2821 gatgcccgct ttcacattcg gcgcaccgca cacaatctgc accaacccgc ccgtacccgc
     2881 atcgactcgg gtaacgttca aacggtcggc atccggatgt ttttcaacgg atttcacttc
     2941 ggcaatcacc acgcccgcaa acgcaggcgc ggcagtttcg gcttcttcca cttccaagcc
     3001 ggacatggtt aacaaatgtt ccagtttatc agcggaaagt tcggtatcgg cttgggtttt
     3061 cagccatgag taggagaatt gcatgttatg ttctcaatta actaattaac tttagatttt
     3121 caagagctag ctaaaccttt ttgaatggct aagtccctat taattacttc tacagaaatg
     3181 ttccataaag aaatcaaccg caaagtatct ttcttattaa attcttgctt tattaattca
     3241 ttcaattgcc caagataatt aagcggcaca tccgggataa ccagtaataa tttgatttct
     3301 atgctacccc attgcaaatt tgaaaaagca ctaggcataa ttgtttcttt gatatcttct
     3361 ctgcctaata ttcccaaaat ttcaacattt aatgcattac accatttttg ataaatctct
     3421 gaaatatatt catcaaaacg ttcaggagat ctttcttgat ttggaatact ttgttttgcc
     3481 tcaacaaata acaaattatc ttcttgcaga tagataaact ctactgtctt aataccttct
     3541 tgccttctct tccggatata tggttctata taaaacaggt tattttttac tagattaaaa
     3601 gttatacctg attcagtgag atttattatt tcactcataa aaacctaacc aatccatgct
     3661 gtttccggag ataaaacacc tatttcttct ctgaacaatc gaatagactc accaataatt
     3721 tcattatccg gcatgctatt ttgtaaattt tcctgcaccc aagtagtctg ttccttttct
     3781 ttgccaggca taaaaacagg aatactttca ctatgctttc tagctaataa acacagcttc
     3841 ttgataacaa aatatgaatg cgtactcata aaaatctgta ttcccaattt ggatttagat
     3901 aaggaaaaca gaatttctag aaattgatta attgctttcg gatgtagatt agcttctggc
     3961 tcatcaataa ataagattga tccaggactc aaaaaccgat ttcctaatag agtgtctaat
     4021 atcccaatct tttttacccc ctctgctgta gaattgatag agaaagtttg tctatctttt
     4081 tcatataccc atgccttctt tttatcattg tagctaacat aaccgccaaa caatgcttct
     4141 aaatcctttc tagcttccgc tgcctcaggg taattcctcc cttttgttgg tttaatatcc
     4201 aatgctttta ccaaatcaat gtaagtagca tcaaaaccaa atctcttttc ttcctctgat
     4261 tttttaatta catcaaatag tgatagaact tctttgggtg gtaaaaaaat agagttagcg
     4321 tttattctgg gagaaagttc gtatagctcc ggtttaatta gctttttggt agttctacca
     4381 aaagaaaaca aaactgaaga actatcttct aaagtcatac ttaacttaag aggccgttcg
     4441 tttggatttt cttttcctct tgtaactaaa tcaccaattt cctctacttg gaacgtccaa
     4501 tatagcttat ctcgcaattc ctcagaaaaa tcacgaatat tctttccttt atgagcttcc
     4561 tcatgacttt ttaaaacact atataatgcc tttaaaataa aagttttccc tttgctattt
     4621 tctgcaataa ttaaatttat tttccctaaa ttatcagcct tcaataagct aatcggtccg
     4681 aatttttcaa gctcaaatga tttaatcata taatccccct aatatatatc aacaatattt
     4741 gtacaagcca cattgtaaaa agacagcaac cacagcacca tagtaaacgc atagcaggcg
     4801 gataatggag cgacatagcg tttcatgatt tagctttctg aggtaggcac aggctgcttt
     4861 tttcattttt aggcagccta cacaatcatt ttacaaactg cttcaaaaaa ttcaaatcgt
     4921 tatcaaaaaa caagcgcaaa tcgttcacgt tgtaacgcag catcgcgaaa cggtcgagac
     4981 cgataccgaa agcgaaaccg gtgtattttt cagaatcgat attcacattt ttcaacacgt
     5041 tgggatgcac catgccgcag ccgccgactt ccagccattt gccgttttcg cccataatgt
     5101 cgatttcggc agacggttcg gtgaacggga agaaagacgg gcggaaacgt acttgcaaat
     5161 catcgcgttc aaagaagcga cggataaaat ccgtgaacac tgctttcaaa tcggcaaaag
     5221 ttacgccctc ttctacccac aaaccttccg cctgatggaa cataggcgag tgcgtggcat
     5281 cgctgtccac acggtaaacg cggccggggg cgataatgcg gatgggcggc tcttttttat
     5341 cgagcatata gcggatttga atcggggaag tgtgcgtacg caaaacatcg ccgttttcaa
     5401 cgtaaaacgt atcctgcatc gcacgggcag gatggtttgc agggatgttc agggcttgga
     5461 aattgtgaaa atcgtcttcg atttcaggcc cgtccgccac ttcgaaaccc attccgtgaa
     5521 agagttcgac cacacgttgc aaggtcaggg ttacgggatg caggctgccg ccttcctgag
     5581 cgcgtccggg cagggtaatg tcgagggctt cggcggcaag tcgggcttgc agcttggctt
     5641 cgttgagggc atcgcgtttg gcattaaaag ccgtctgaaa ccggtttttg cattcattga
     5701 tatgcgcacc tatggttttg cgctcttcag gcgacatttg ccccaaagtt ttcagaagtc
     5761 cggtcaactc gccggttttg ccaagataac gggctttgat ttgttctaga gcgttgaagt
     5821 cttgcgcagc ttctactgcg gcaatgcctt ctgcaacgat gcggtttaca ttttccataa
     5881 tatcaagtct gtcaattaat gatataccac ctgaaacaac gaggccgcct gtatagcctg
     5941 acatatcaga ctcttcagac gaccttattt tcttcacggt caatccattg gataaatttt
     6001 ctgtatag
//
LOCUS       DAXHSF010000125         6000 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.125, whole genome shotgun sequence.
ACCESSION   DAXHSF010000125 DAXHSF010000000
VERSION     DAXHSF010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6000
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.125"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..108
                     /locus_tag="ACFM8S_001606"
     CDS             <1..108
                     /locus_tag="ACFM8S_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HFC6314659.1"
                     /translation="AAYFGLIKVSAQSHLKAMCLNLLKAANRLSAPAAA"
     gene            complement(255..416)
                     /locus_tag="ACFM8S_001607"
     CDS             complement(255..416)
                     /locus_tag="ACFM8S_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314660.1"
                     /translation="MPSENHETGFRRPYILFFLVFRYMKSSLKMGRKAGFAAGCSLIF
                     NYIAIDMKS"
     gene            complement(442..1509)
                     /gene="murG"
                     /locus_tag="ACFM8S_001608"
     CDS             complement(442..1509)
                     /gene="murG"
                     /locus_tag="ACFM8S_001608"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFC6314661.1"
                     /translation="MGGKTFMLMAGGTGGHIFPALAVADSLRARGHHVIWLGSKDSME
                     ERIVPQYGIRLETLAIKGVRGNGIKRKLMLPVTLYQTVREAQRIIRKHRVECVIGFGG
                     FVTFPGGLAAKLLGVPIVIHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNP
                     VRADISNLPVPAERFQGREGRLKILVVGGSLGADVLNKTVPQALALLPDNARLQMYHQ
                     SGRNKLGSLQADYDALGVKAECVEFITDMVSAYRDADLVICRAGALTIAELTAAGLGA
                     LLVPYPHAVDDHQTANARFMVQAEAGLLLPQTQLTAEKLAEILGGLNREKCLKWAENA
                     RTLALPHSADDVAEAAIECTA"
     gene            complement(1513..2838)
                     /locus_tag="ACFM8S_001609"
     CDS             complement(1513..2838)
                     /locus_tag="ACFM8S_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFC6314662.1"
                     /translation="MQTDWQVVFLKISEVLVKVGDGVHTLLLDRPIVRDGRKFDAPLL
                     WMVVLMTAFSLLMIYSASVYLASKEGGDQFFYLTRQAGFVVAGLIASGFLWFLCRMRT
                     WRRLVPWIFALSGLLLVVVLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLF
                     TRREEVLRSMESLGWQSIWRGTANLIMSATNPQARRETLEMYGRFRAIILPIMLVAFG
                     LVLIMVQPDFGSFVVITVIAVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQR
                     VVAFLDPWKDPQGAGYQLTHSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIA
                     EEFGFFGMCVLIFCYGWLVVRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGV
                     NIGALPTKGLTLPLMSYGGSSVFFMLISMMLLLRIDYENRRKMRGYRVE"
     gene            complement(2964..4301)
                     /gene="murD"
                     /locus_tag="ACFM8S_001610"
     CDS             complement(2964..4301)
                     /gene="murD"
                     /locus_tag="ACFM8S_001610"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFC6314663.1"
                     /translation="MTFQNKKILVAGLGGTGISMIAYLRKNGAEVAAYDAELKPERVS
                     QIGKMFDGLVFYTGRLKDALDNGFDILALSPGISERQPDIEAFKQNGGRVLGDIELLA
                     DIVNRRGDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAEWQREGKK
                     ADVWVLELSSFQLENTESLRPTAATVLNISEDHLDRYDDLLDYAHTKAKIFRGDGVQV
                     LNADDAFCRAMKRAGREVKWFSLEHEADFWLERETGRLKQGNEDLIATQDIPLQGLHN
                     ATNVMAAVALCEAVGLPREALLEHVKTFQGLPHRVEKIGEKNGVVFIDDSKGTNVGAT
                     AAAIAGLQNPLFVILGGMGKGQDFTPLRDALAGKAKGVFLIGVDAPQIRRDLDGCDLN
                     MTDCATLEEAVQTAYAQAEAGDIVLLSPACASFDMFKGYAHRSEVFIGAFKAL"
     gene            complement(4389..4769)
                     /locus_tag="ACFM8S_001611"
     CDS             complement(4389..4769)
                     /locus_tag="ACFM8S_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC6314664.1"
                     /translation="MKQTVKWLAAALIALGLNQAVWADDVSDFRENLQAAEQGNAAAQ
                     YNLGAMYYKGQGVRRDDAEAVRWYRQAAEQGLAQAQNNLGVMYAERRGVRQDRALAQE
                     WLGKACQNGNQDGCDNDQRLKAGY"
     gene            complement(4895..5977)
                     /gene="mraY"
                     /locus_tag="ACFM8S_001612"
     CDS             complement(4895..5977)
                     /gene="mraY"
                     /locus_tag="ACFM8S_001612"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFC6314665.1"
                     /translation="MFLWLAHFSNWLTGLNIFQYTTFRAVMAALTALAFSLMFGPWTI
                     RRLTALKCGQAVRTDGPQTHLVKNGTPTMGGSLILTAITVSTLLWGNWANPYIWILLG
                     VLLATGALGFYDDWRKVVYKDPNGVSAKFKMVWQSSVAIIAGLALFYLAANSANNILI
                     VPFFKQIALPLGVVGFLVLSYLTIVGTSNAVNLTDGLDGLAAFPVVLVAAGLAIFAYV
                     SGHYQFSQYLQLPYVAGANEVAIFCTAMCGACLGFLWFNAYPAQVFMGDVGALALGAA
                     LGTVAVIVRQEFVLVIMGGLFVVEAVSVMLQVGWYKKTKKRIFLMAPIHHHYEQKGWK
                     ETQVVVRFWIITIVLVLIGLSTLKIR"
BASE COUNT         1426 a         1743 c         1555 g         1276 t
ORIGIN      
        1 gcagcctatt tcggactgat taaagtgagt gcgcaaagcc acctgaaggc gatgtgtttg
       61 aacctgttga aagcggccaa caggttaagt gcgcccgctg ccgcctaaaa ggcagcccgg
      121 atgcctgatt atcgggtgtc cgtggaggat taagggggta tttgggtaga attaggaggt
      181 atttggggcg aaaatagacg aaaacctgtg tttgggtttc ggctgtcggg agggaaagga
      241 attttgcaaa ggtctcagga tttcatatcg atggcaatat agttaaaaat aaggctgcac
      301 ccagcggcaa accctgcttt cctgcccatt ttcaaactcg atttcatata ccgaaacacc
      361 agaaaaaaca aaatataagg ccgtctgaaa ccggtttcgt ggttttcaga cggcatctgt
      421 ttcgtttatc gcatcatgtc atcatgccgt acactcgata gcggcttccg ccacgtcgtc
      481 cgcactgtgc ggcagtgcca acgtacgggc gttttctgcc catttgaggc atttttcgcg
      541 gtttaagccg ccgagaatct cggcgagttt ttccgccgtc aactgggttt gcggcaacag
      601 caatcccgcc tccgcctgca ccataaaacg cgcgttggcg gtttggtgat cgtcaaccgc
      661 gtgggggtac ggaacgagca acgcacccaa tcccgccgcc gtcaactcgg caatcgtcag
      721 cgcgccggca cggcaaatca ccaaatcggc atcgcggtag gcggacacca tatcggtaat
      781 aaattccacg cattcggctt tcacgcccag cgcgtcgtaa tccgcctgca agctgcccag
      841 cttgttgcgc cccgactggt ggtacatctg cagacgcgca ttgtcgggca gcaaagccaa
      901 tgcctgcggt acggttttgt tcaaaacgtc cgcgcccaaa ctgccgccga ccaccaaaat
      961 tttcagacgg ccttcacgcc cttggaagcg ttcggcaggc acgggcaggt tgctaatatc
     1021 ggcgcggacg gggttgccga ccaagccgcc ttcgtggctg aacgctttcg gaaaagcgta
     1081 caacacccgc ttcgcccagc gcgacaggtg gcggttggac aaacctgcca cggcgttttg
     1141 ctcgtgaatc acaatcggca cgcctaatag cttcgccgcc aaaccgccgg ggaaggtaac
     1201 gaagccgccg aagccgatga cgcactcgac acggtgtttg cggataatcc gctgcgcttc
     1261 gcggacggtt tgatacaaag taaccggcag catcagtttg cgtttgatgc cgttgccgcg
     1321 cacgccttta atcgccagcg tttccaagcg tatgccgtat tgcggcacga tacgctcttc
     1381 catcgaatcc ttgctgccca gccaaatcac atgatggccg cgcgcgcgca atgaatccgc
     1441 caccgccagc gcggggaaaa tatgtccgcc cgttccgccc gccatcagca taaaggtttt
     1501 accgcccatg atttactcca cccgataacc gcgcattttc cggcggtttt cataatctat
     1561 acgcaacagc agcatcatgc tgatcagcat gaaaaagact gacgaaccgc cataggacat
     1621 caacggcagc gtcagacctt tggtcggcaa agcaccgatg ttcacaccga tattgaagaa
     1681 actttggata ccgatccaaa tgccgatacc cgaagcgata taggcgttga aagtcaaacc
     1741 caaatcgcgc gactgcttgc cgatggaaaa cgcccgcacc accagccagc cgtaacagaa
     1801 tatcagcacg cacataccga agaaaccgaa ttcttcggcg atgatggcaa aaataaaatc
     1861 ggtatgcgct tccggcagaa agccgcgttt gctcaaactc gcacccaaac ccataccgaa
     1921 ccactctccg cgcccgattg ccatcagaga gtgggtaagc tggtagccgg caccctgcgg
     1981 gtctttccac gggtccaaaa atgccactac ccgctgcaca cggtagggag cggcggtaat
     2041 catcagcacc atcccgccca agacgctgcc taccaggacg aaaaaatatt tccacggcaa
     2101 tcctgccaaa aacagcattc caacggcaat gacggtaatg acgacaaacg aaccgaaatc
     2161 cggctgtacc attatcagca ccaaaccgaa cgccaccagc ataatcggca ggatgatcgc
     2221 ccggaaacgg ccgtacattt ctaatgtttc acgacgtgcc tgcggattgg tggcggacat
     2281 gatcagattg gccgtccccc gccaaatcga ctgccaaccc aaactttcca tgctgcgcaa
     2341 cacttcttca cggcgcgtga acaggcttgc caaataaagg atgaccgcca gcttgaacag
     2401 ctcggtcggc tggaaattca acggacccaa aggtatccaa cgggtcgcgc cattgatttc
     2461 gcgcccggca atcaatacga ctaccagcaa caggccggat agggcaaaaa tccacggcac
     2521 aagccgccgc catgtcctca tcctgcaaag aaaccataaa aaaccgctcg ctatcaagcc
     2581 ggcaacgacg aaccccgcct gtctggtcaa atagaaaaac tgatcgccgc cttcttttga
     2641 tgccaaatac acagaagccg aataaatcat cagcaggctg aacgccgtca tcagcaccac
     2701 catccacaaa agcggcgcgt cgaatttcct gccgtcgcgc acaatcggcc tgtcgagcag
     2761 cagagtgtgg acaccgtcgc ccacttttac caatacttcc gaaatcttca aaaaaaccac
     2821 ctgccagtct gtttgcacct gccgcaaagg gcaaaaattt cagacggcag acaatgccgt
     2881 ccgaacatac gatacatccc aaatcggtat tctaaatctt tacttgccgc ccaacaatga
     2941 cggcgtttgc gtttcagacg gtatcacaaa gccttaaacg ccccgataaa cacttccgaa
     3001 cggtgcgcat agcctttaaa catatcgaag cttgcgcagg cggggctgag caacacgatg
     3061 tcgcccgctt cggcttgggc atatgccgtc tgaaccgctt cttccaaagt ggcgcagtcg
     3121 gtcatattca gatcgcagcc gtccaaatcg cggcggattt gcggcgcatc gacaccaatc
     3181 aggaacacgc cttttgcctt gccggcaagc gcgtcgcgca ggggcgtgaa gtcctgccct
     3241 ttgcccatgc cgcctaaaat cacgaagagc ggattttgca aaccggcaat cgcggcggcg
     3301 gtcgcaccga cgttcgtgcc tttgctgtca tcgataaaca ccacgccatt tttctcgccg
     3361 attttttcca cgcggtgcgg caggccttga aaggttttga cgtgttcgag caatgcttcg
     3421 cgcggcaaac cgacagcttc gcacaaggca acggcagcca tcacgttggt ggcgttgtgc
     3481 aagccttgca acggaatatc ttgcgtggca atcaaatctt cattgccttg tttcaggcgg
     3541 cctgtctcgc gttccaacca aaaatcggct tcgtgttcca acgaaaacca ttttacctcg
     3601 cgccctgccc gtttcatggc acggcagaac gcatcgtccg cattcaaaac ctgcacaccg
     3661 tcgccacgga aaatcttggc tttggtatgc gcgtagtcga gcaagtcgtc gtagcggtcg
     3721 agatggtctt cggaaatgtt cagtaccgtc gccgcagtcg ggcgcaggct ttcggtgttt
     3781 tccagttgga agctggaaag ctccaacacc cacacgtccg cctttttgcc ttcgcgctgc
     3841 cattccgcct ccaaaaccgg cgtgccgata ttgcccgcga taacggtatc cagcccgcac
     3901 ttgatgcaga gatagccgac caggctcgtt accgtggttt tgccgttgct gccggtaatc
     3961 gcaattacct tgtcgcctcg gcggttcaca atgtccgcca gcaattcgat gtcgcccaac
     4021 acgcgtccgc cgttttgctt gaacgcctcg atatccggct gccgctcgct gatgccgggg
     4081 ctgagtgcca gaatatcgaa accgttgtcc agcgcatctt tcagacggcc cgtataaaac
     4141 accagcccgt caaacatctt accgatttgc gacacccgct ccggtttcag ctccgcatca
     4201 taagcggcaa cctccgcgcc gtttttgcgt aggtaggcaa tcatggaaat acccgtgccg
     4261 ccgagtccgg cgacgaggat ttttttgttt tggaaagtca ttttggtttt gtcctaaaac
     4321 aaatcatatt gggcaggagg gaatgtccgc tcctgctcaa gccgctttca gacggcatcg
     4381 cgagccgatt aataacccgc cttcaggcgt tggtcattgt cgcagccgtc ttggtttccg
     4441 ttttgacaag ccttgccaag ccattcttgt gcaagggcgc ggtcttggcg cacgccgcgt
     4501 ctttcggcat acatcacgcc caaattgttt tgggcttggg ctaacccctg ttccgccgcc
     4561 tgccgatacc atctgaccgc ttcagcatca tcccggcgca cgccttgtcc tttgtaatac
     4621 attgcgccca aattgtattg ggctgctgca tttccctgct ctgccgcctg caagttttcc
     4681 cgaaaatccg atacgtcatc cgcccacacc gcttggttca agcccaaggc aatcagggcg
     4741 gcggcaagcc atttgactgt ctgtttcatg gttttacttc tgttttagta taaggcgggt
     4801 ttcagccacc gttaacgata gggctgggcg gattgtcgcc gcaggtttat tgcgcgttca
     4861 aatgccgtct gaaagatgtt cagacggcat aggttcagcg gattttgagg gtactcaaac
     4921 cgatcaacac caagacgatg gtaataatcc aaaagcggac gacgacttgg gtttctttcc
     4981 agcctttttg ttcgtaatgg tggtggatgg gcgccatcag gaagatgcgt tttttggttt
     5041 tcttgtacca gccgacttga agcataacgg acacggcttc gacgacgaac agaccgccca
     5101 taatcactaa aacaaactct tggcggacga tgacggcaac ggtaccgagt gccgcgccca
     5161 atgccagcgc gccgacatcg cccataaaga cttgcgcggg ataggcgttg aaccacaaga
     5221 aaccgaggca cgcgccgcac atggcggtac agaatatagc gacttcgttc gcaccggcga
     5281 catagggaag ctggaggtat tgggaaaatt ggtagtgtcc gctgacgtag gcgaaaatgg
     5341 cgaggccggc agcaacgagg acgacgggga aggcggcaag gccgtccaag ccgtcggtga
     5401 ggttgacggc attggatgtg ccgacgatgg tcaggtaaga caacaccaaa aagccgacca
     5461 cgcccagcgg cagggcgatt tgtttgaaga acgggacaat caaaatattg ttggcggaat
     5521 tggcggcaag gtaaaacaat gccaaaccgg cgataatggc aacgcttgac tgccacacca
     5581 ttttgaattt ggcggacacg ccgttggggt ctttatagac gactttgcgc cagtcgtcgt
     5641 aaaaaccgag tgcgcccgtg gcgagcaata cgcccaagag aatccagata tacgggtttg
     5701 cccagttgcc ccacaacagg gtggacacgg taatggcggt cagaatcagc gaaccgccca
     5761 tcgtcggcgt gccgtttttg acgaggtggg tttgcggacc gtcggtacgc actgcctgcc
     5821 cgcatttgag cgcggtcagc ctgcgtatcg tccacgggcc gaacatcagg gaaaacgcca
     5881 aggcggtcaa cgccgccatg acggcgcgga atgtggtgta ttgaaaaata ttcagaccgg
     5941 ttaaccagtt gctgaaatgt gcgagccata aaaacatggg gcttcctttt tttgttttgt
//
LOCUS       DAXHSF010000126         5893 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.126, whole genome shotgun sequence.
ACCESSION   DAXHSF010000126 DAXHSF010000000
VERSION     DAXHSF010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5893
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.126"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            72..998
                     /gene="fabD"
                     /locus_tag="ACFM8S_001613"
     CDS             72..998
                     /gene="fabD"
                     /locus_tag="ACFM8S_001613"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFC6314666.1"
                     /translation="MSFAFFFPGQGSQSLGMMNGFAEHAIVKNTFDEASAILGQDLWA
                     MINGSDAEIIGQTVNTQPIMLAAGVAVYRAYSEAGGKTPAAVAGHSLGEYTALVAAGA
                     LDFADAVKLVRLRAELMQSAVPQGVGAMAAILGLEDEQVKQICAESTQGEVVEAVNFN
                     SPGQVVIAGNTAAVERAMLAAKEAGAKRALPLPVSVPSHCSLMKPAADKLAEALKTVE
                     IKQPQIRVIHNADVAAYDDADKIKDALVRQLYSPVRWTETVNALVSDGIAESAECGPG
                     KVLAGLAKRINKAAACSALTDAGQVAAFIETH"
     gene            1136..3001
                     /gene="msbA"
                     /locus_tag="ACFM8S_001614"
     CDS             1136..3001
                     /gene="msbA"
                     /locus_tag="ACFM8S_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HFC6314667.1"
                     /translation="MIEKLTFGLFKKEDARSFMRLMAYVRPYKIRIIAALIAIFGVAA
                     TESYLAAFIAPLINHGFSAPAAPPELSAAAGIISTLQNWREQFTYMVWGTENKIWTVP
                     LFLIILVVIRGICRFTSTYLMTWVSVMTISKIRKDMFAKMLTLSSRYHQETPSGTVLM
                     NMLNLTEQSVSNASDIFTVLTRDTMIVTGLTIVLLYLNWQLSLIVVLMFPLLSLLSRY
                     YRDRLKHVISDSQKSIGTMNNVIAETHQGHRVVKLFNGQAQAANRFDAVNRTIVRLSK
                     KITQATAAHSPFSELIASIALAVVIFIALWQSQNGYTTIGEFMAFIVAMLQMYAPIKS
                     LANISIPMQTMFLAADGVCAFLDTPPEQDKGTLAPQRVEGRISFRNVDVEYRSDGIKA
                     LDNFNLDIRQGERVALVGRSGSGKSTVVNLLPRFVEPSAGNICIDGIDIADIKLDCLR
                     AQFALVSQDVFLFDDTLFENVRYSRPDAGEAEVLSALQAANLQSLIDSSPLGLHQPIG
                     SNGSNLSGGQRQRVAIARAILKDAPILLLDEATSALDNESERLVQQALERLMENRTGI
                     IVAHRLTTIEGADRIIVMDDGKIIEQGTHEQLMSQNGYYTMLRNISNKDAAVRTA"
     gene            3101..4666
                     /gene="guaA"
                     /locus_tag="ACFM8S_001615"
     CDS             3101..4666
                     /gene="guaA"
                     /locus_tag="ACFM8S_001615"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFC6314668.1"
                     /translation="MTQDKILILDFGSQVTQLIARRVREAHVYCELHSFDMPLDEIKA
                     FNPKGIILSGGPNSVYESDYQADTGIFDLGIPVLGICYGMQFMAHHLGGEVQPGNQRE
                     FGYAQVKTIDSELTRDIFDDAPNTLDVWMSHGDKVSKLPDGFAVIGNTPSCPIAMMEN
                     AEKQFYGIQFHPEVTHTKQGRALLNRFVLDICGAQPGWTMPNYIEEAVAKIREQVGSD
                     EVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGKMVMDMFARNLGVKVI
                     HVDAEGQFMAKLAGVTDPEKKRKIIGAEFIEVFDAKEKKLTNAKWLAQGTIYPDVIES
                     AGAKTQKAHAIKSHHNVGGLPENMKLKLLEPLRDLFKDEVRELGVALGLPREMVYRHP
                     FPGPGLGVRILGEVKKEYADLLRQADDIFIQELRNTTDENGTSWYDLTSQAFAVFLPV
                     KSVGVMGDGRTYDYVVALRAVITSDFMTAHWAELPYSLLGRVSNRIINEVKGINRVVY
                     DVSGKPPATIEWE"
     gene            4728..5474
                     /gene="fabG"
                     /locus_tag="ACFM8S_001616"
     CDS             4728..5474
                     /gene="fabG"
                     /locus_tag="ACFM8S_001616"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFC6314669.1"
                     /translation="MSTQDLSGKIALVTGASRGIGAAIADTLAAAGAKVIGTATSESG
                     AAAISERLAQWGGEGRVLNSAEPETVENLIADIEKTFGKLDILVNNAGITRDNLLMRM
                     KEEEWDDIMQVNLKSVFRASKAVLRGMMKQRAGRIINITSVVGVMGNAGQTNYAAAKA
                     GLIGFSKSMAREVGSRGITVNCVAPGFIDTDMTRALPEETRQTFTAQTALGRFGDAQD
                     IADAVLFLASDQAKYITGQTLHVNGGMLMP"
     gene            complement(5736..>5893)
                     /locus_tag="ACFM8S_001617"
     CDS             complement(5736..>5893)
                     /locus_tag="ACFM8S_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6314670.1"
                     /translation="VEQSFGTLHRKFRYARAAYFGLIKVSAQSHLKAMCLNLLKAANR
                     LSAPVAA"
BASE COUNT         1418 a         1895 c         1433 g         1147 t
ORIGIN      
        1 ttttgtcccg cctgaatttt tcgtaaaact cgggcagaat acctgattat ccaaccaaat
       61 aaaggaatac tatgtctttt gccttctttt ttcccggaca aggttcccaa agcctcggta
      121 tgatgaacgg ctttgccgaa cacgccatcg tcaaaaacac atttgacgaa gcctccgcca
      181 tattggggca ggacttgtgg gcgatgatta acggcagcga tgccgaaatc atcggtcaaa
      241 ccgtcaacac ccagcccatt atgctcgccg ccggcgttgc cgtttaccgc gcctattcgg
      301 aagcgggcgg caaaacgcct gccgccgttg ccggacacag cctcggcgaa tacaccgcac
      361 tcgttgccgc cggcgcattg gattttgccg acgcggtcaa actcgtgcgc ctgcgcgccg
      421 aactgatgca gtctgccgta ccgcaaggcg tgggcgcaat ggcggcgatt ctcggcttgg
      481 aagatgaaca ggtaaaacaa atttgcgccg aatccacaca aggcgaagtg gttgaagccg
      541 tcaacttcaa ctcgcccgga caagtcgtga ttgcaggcaa taccgccgcc gtcgagcgcg
      601 ctatgcttgc cgccaaagaa gccggcgcca aacgcgccct gccgctgccc gtgtccgtac
      661 cttcccattg cagcctgatg aaacccgccg ccgacaaact cgccgaagcc ctgaaaaccg
      721 ttgaaatcaa gcagccgcaa atccgcgtca tccacaacgc cgacgttgcc gcctacgatg
      781 atgccgacaa aatcaaagac gcgctcgtcc gccagcttta cagccccgta cgctggacag
      841 aaaccgtcaa cgcccttgtt tcagacggca ttgccgaatc cgccgaatgc ggcccgggca
      901 aagtgttggc gggcttggca aaacgcatca acaaggccgc cgcgtgcagc gcactgaccg
      961 atgccggaca ggttgccgcc tttatcgaaa cgcactgact tcgttccgca aaaagcagcc
     1021 tgccctcttc aggctgcttt tcacgtccga acggcggcag ccgcatattt gcgctataat
     1081 tgatgacgac caaaccaccg acagcggctg ccgttgcagt tcccgcccca ccgacatgat
     1141 agaaaaactg actttcggac tgtttaaaaa agaagacgcg cgcagcttta tgcgcctgat
     1201 ggcatacgtc cgcccctaca aaatccgcat cattgccgcc ctgattgcca ttttcggcgt
     1261 tgccgccacc gaaagctacc ttgccgcctt catcgccccc ctgattaacc acggcttttc
     1321 cgcacccgcc gcgccgcccg agctgtctgc cgccgccggc atcatttcca ccctgcaaaa
     1381 ctggcgcgaa cagtttacct atatggtttg ggggacggaa aacaaaatct ggaccgtccc
     1441 cctcttcctc atcatcctcg tcgtcatccg tggcatctgc cgctttacca gcacctatct
     1501 gatgacttgg gtctccgtga tgaccatcag caaaatccgc aaagatatgt ttgccaaaat
     1561 gctgaccctt tcctcccgct accatcagga aacgccgtcc ggcaccgtac tgatgaatat
     1621 gctcaacctg accgaacagt cggtcagtaa cgccagcgac atcttcaccg tcctcacgcg
     1681 cgacacgatg atcgttaccg gcctgaccat cgtcctgctt tacctcaact ggcagctcag
     1741 cctcatcgtt gtcctgatgt tccccctgct ctccctgctc tcgcgctact accgcgaccg
     1801 tctgaagcac gtcatttccg actcgcaaaa aagcataggc acgatgaaca acgtgattgc
     1861 cgaaacccat cagggacacc gcgtcgtcaa gctgttcaac gggcaggcgc aggcggcaaa
     1921 ccggtttgac gcggtcaacc gcaccatcgt ccgcctcagc aaaaaaatca cgcaggcaac
     1981 ggcggcacat tccccgttca gcgaactgat tgcctcgatt gccctcgccg ttgtcatctt
     2041 catcgccctg tggcaaagcc aaaacggcta caccaccatc ggcgaattta tggcattcat
     2101 cgtcgcgatg ctgcaaatgt acgcccccat caaaagcctt gccaacatca gcatccctat
     2161 gcagacgatg ttcctcgccg ccgacggcgt atgtgcattt ctcgacaccc cgcccgaaca
     2221 ggacaaaggc acgctcgcgc cgcagcgtgt cgaagggcgt atcagcttcc gcaacgtcga
     2281 tgtcgaatac cgttcggacg gcatcaaagc cctcgacaac ttcaacctcg acatcagaca
     2341 aggcgaacgc gtcgccttgg tcggacgttc cggcagcggc aaatccaccg tcgtcaacct
     2401 gctgccccgc tttgtcgaac cgtctgccgg caacatctgc atagacggta tcgacatcgc
     2461 cgacatcaaa ctcgactgcc tgcgcgccca attcgccctc gtctcccaag acgtattcct
     2521 gtttgacgac accctgtttg aaaacgtccg atacagccgt cccgacgcag gcgaagccga
     2581 agtcctgtcc gccctccaag ccgccaacct gcaaagcctg attgacagct ccccgctcgg
     2641 actgcaccag cccatcggat cgaacggcag caacttgtcc ggcggacagc ggcaacgcgt
     2701 cgccatcgcc cgcgccattt tgaaagacgc gccgatatta ttattggacg aagccaccag
     2761 cgcattagac aacgaatccg aacgcctcgt ccaacaagcg ctcgaacgcc tgatggaaaa
     2821 ccgcaccggc atcatcgtcg cccaccgcct gaccaccatc gaaggggccg accgcatcat
     2881 cgtaatggac gacggcaaaa tcatcgaaca aggcacacac gaacaactga tgtcccaaaa
     2941 cggctactac acgatgttac gcaatatctc aaacaaagat gccgccgtcc ggacggcata
     3001 aacaaaatgc cgtccgaaat ggtacaatcg ccccgaccct ttcagacggc actatatccg
     3061 ccgacccatc cgattatctt caatcactgt aaaacccatt atgacccaag acaaaatcct
     3121 catcctcgac ttcggttcgc aagttaccca gctgattgcc cgccgcgtgc gcgaagccca
     3181 cgtttactgc gaactgcatt ccttcgatat gcctttggac gaaatcaaag ccttcaaccc
     3241 caaaggcatc atcctttccg gcggccctaa ttccgtttac gaatccgact atcaggccga
     3301 taccggtatt tttgatttgg gcattccggt tttgggcatc tgctacggaa tgcagtttat
     3361 ggcgcaccac ttgggcggcg aagtgcagcc cggcaaccag cgcgaattcg gttatgcgca
     3421 agtcaaaacc atcgacagcg aactgacccg cgatattttc gacgacgcgc ccaacacact
     3481 cgacgtatgg atgagccacg gcgacaaagt gtccaaactg cccgacggct tcgccgtcat
     3541 cggcaacacc ccgtcctgcc cgattgccat gatggaaaac gccgaaaaac aattctacgg
     3601 catccagttc caccccgaag ttacccacac caaacaaggc cgcgccctgt tgaaccgctt
     3661 tgtcttggat atttgcggcg cacaaccggg ctggacgatg ccgaactaca tcgaagaagc
     3721 cgttgccaaa atccgcgaac aggtcggcag cgacgaagtg attttaggtt tgtcaggcgg
     3781 cgtagactct tccgtagccg ccgcgctgat tcaccgcgcc atcggcgacc aactgacctg
     3841 cgtgttcgtc gatcacggtt tgttgcgcct gaacgaaggc aaaatggtga tggatatgtt
     3901 cgcccgcaac ttgggtgtga aagtgattca cgtcgatgcc gaagggcagt ttatggcgaa
     3961 actcgccggc gttaccgacc ctgagaaaaa acgcaaaatc atcggcgcgg aatttatcga
     4021 agtatttgat gccaaagaga aaaaactcac caacgccaaa tggctggcgc aaggcacgat
     4081 ttaccccgac gtaatcgaat ccgcaggcgc gaaaacccaa aaagcccacg ccatcaaatc
     4141 gcaccacaac gtcggcggtc tgcccgaaaa tatgaagctc aagctgcttg agccgttgcg
     4201 cgacttgttt aaagacgaag tgcgcgaact gggcgttgct ttgggtctgc cgcgcgaaat
     4261 ggtgtaccgc catccgttcc ccggccccgg tttgggcgtg cgcatcttgg gcgaagtgaa
     4321 aaaagaatac gccgacttat tgcgtcaagc agacgatatt tttattcaag aattacgcaa
     4381 caccaccgac gaaaacggca cgtcttggta cgacctgacc agccaagcct tcgccgtgtt
     4441 cctgcctgtg aaatccgtcg gcgtaatggg cgacggacgc acttacgact acgtcgtcgc
     4501 actgcgcgca gtcatcacca gcgactttat gaccgcacat tgggctgagt tgccttactc
     4561 actgctcgga cgcgtgtcca accgcatcat caacgaagtc aaaggcatca accgcgtggt
     4621 gtacgatgtc agcggcaaac cgcctgccac catcgagtgg gaataaccag caaacagggc
     4681 tgcaccgtcc ggcgcagtct ttcgattatc ggaaaaaagg aaaaaatatg agcacacaag
     4741 atttaagcgg caaaatcgct ttggtaacag gcgcctcgcg cggtatcggt gcagcaattg
     4801 ccgacacact ggcggcagcc ggtgccaaag tcatcggtac ggcgaccagt gagagcggtg
     4861 cggcggcgat tagcgagcgg ttggcgcaat ggggcggcga aggccgcgta ttaaattccg
     4921 ccgaacccga aaccgtcgaa aacctgattg ccgacatcga aaaaacgttc ggcaaactcg
     4981 atattctggt caacaacgcc ggcatcaccc gcgacaacct cctgatgcgt atgaaagaag
     5041 aagagtggga cgacatcatg caggtcaacc tcaaatccgt ctttcgcgcc tccaaagccg
     5101 tcttgcgcgg catgatgaag cagcgcgccg gccgtatcat caacatcaca tccgtcgtcg
     5161 gcgtgatggg caatgccggt caaaccaact atgccgcggc aaaagcaggc ttaatcggtt
     5221 tctccaaatc catggcgcgc gaagtcggca gccggggcat taccgtcaac tgcgtcgccc
     5281 ccggctttat cgataccgac atgacccgcg ccctgccgga agaaacccgc caaaccttta
     5341 ccgcccaaac cgccttgggc agattcggcg acgcgcaaga cattgccgat gcagtcttgt
     5401 tcttggcttc cgatcaggca aaatacatta ccggtcaaac gctgcacgtc aacggcggca
     5461 tgctgatgcc ctgacgggca gccgtaccga caatgccgtc tgaagccctt tcagacggca
     5521 tttgcattcc caaacaaaat gaacacacgc cacactccgc cctgcccctg cggttcaggc
     5581 acaagctgag gcctttgcaa aattcctttc cctcccgaca gccgaaaccc aaacacaggt
     5641 tttcggctgt tttcgcccca aatacctcct aattctaccc aaatatcccc ttaatcctcc
     5701 ccggataccc gataatcagg catccggacc gccttttagg cggcaacagg cgcacttagc
     5761 ctgttggcgg ctttcaacag gttcaaacac atcgccttca ggtggctttg cgcactcact
     5821 ttaatcagcc cgaaataggc tgcccgggcg tagcggaatt tgcggtgcag cgtaccgaag
     5881 ctctgttcga cca
//
LOCUS       DAXHSF010000127         5855 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.127, whole genome shotgun sequence.
ACCESSION   DAXHSF010000127 DAXHSF010000000
VERSION     DAXHSF010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5855
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.127"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(<1..561)
                     /locus_tag="ACFM8S_001618"
     CDS             complement(<1..561)
                     /locus_tag="ACFM8S_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor"
                     /protein_id="HFC6314671.1"
                     /translation="MEDKQGMTKAVAGVMTDALADGRKPATASNLPPLSNRGGNRGGT
                     ETETAGRVQEVFECYETYITDGKGNLLGVPLRRGVSDSAFIDQISFSFHEKPFFDKYG
                     VRVSLLEDEDFIRAASMLAEEVFGFGIYKESKGSGGRFYERCWLMGSEDVLYGRVHFG
                     GQNNTILFELTGTGCGVAKEGWESRLF"
     gene            663..973
                     /locus_tag="ACFM8S_001619"
                     /pseudo
     CDS             663..973
                     /locus_tag="ACFM8S_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684347.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(1030..2088)
                     /gene="trpD"
                     /locus_tag="ACFM8S_001620"
     CDS             complement(1030..2088)
                     /gene="trpD"
                     /locus_tag="ACFM8S_001620"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HFC6314672.1"
                     /translation="MITPQQAIERLISNNELFYDEMTDLMRQIMRGQVLPEQIAAILT
                     GLRIKVETVSEITAAAAVMREFATKVPLENAEGLVDIVGTGGDGAKTFNISTTSMFVA
                     AAAGAKVAKHGGRSVSSSSGAADVVEQMGANLNLTPEQVAQSIRQTGIGFMFAPNHHS
                     AMRHVAPVRRSLGFRSIFNILGPLTNPAGAPNQLLGVFHTDLCGILSRVLQQLGSKHV
                     LVVCGEGGLDEITLTGKTRVAELKDGKISEYDIRPEDFGIETRRNLDEIKVANTQESL
                     LKMNEVLDGKEGAARDIVLLNTAAALYAGNVAASLSDGISAAREAIDSGRAKSKKEEF
                     VGFQPQQRCHFLGKMELG"
     gene            complement(2152..2742)
                     /locus_tag="ACFM8S_001621"
     CDS             complement(2152..2742)
                     /locus_tag="ACFM8S_001621"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HFC6314673.1"
                     /translation="MLLFIDNYDSFTYNIVQYFTELGQEVAVRRNDDITLEEIEALNP
                     QYLVIGPGPCSPKEAGISVAAMRHFAGRLPIMGVCLGHQTIGEAFGGRIVRAKTLMHG
                     KVSPVSHSGKGMFKGLPNPVTCTRYHSLVIDRNTMPECLEVTAWTEDGEIMGVRHKEY
                     AVEGVQFHPEALLTEHGHDMLNNFLIEFQNFKPQKI"
     gene            3164..5440
                     /gene="znuD"
                     /locus_tag="ACFM8S_001622"
                     /gene_synonym="tdfJ"
     CDS             3164..5440
                     /gene="znuD"
                     /locus_tag="ACFM8S_001622"
                     /gene_synonym="tdfJ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_118820519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent zinc receptor ZnuD"
                     /protein_id="HFC6314674.1"
                     /translation="MAQTTLKPIVLSILLINTPLLAQAHETDRSVDLETVSVVGKSRP
                     RATSGLLHTSTASDKIISGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTG
                     RRIKVLNHHGETGDMADFSPDHAIMVDTALSQQVEILRGPVTLLYSSGNVAGLVDVAD
                     GKIPEKMPENGVSGELGLRLSSGNLEKLTSGGINIGLGKNFVLHTEGLYRKSGDYAVP
                     RYRNLKRLPDSHADSQTGSIGLSWVGEKGFIGAAYSDRRDQYGLPAHSHEYDDCHADI
                     IWQKSLINKRYLQLYPHLLTEEDIDYDNPGLSCGFHDDDDAHAHAHNGKPWIDLRNKR
                     YELRAEWKQPFPGFEALRVHLNRNDYRHDEKAGDAVENFFNNQTQNARIELRHQPIGR
                     LKGSWGVQYLQQKSSALSATSEAVKQPMLLDNKVQHYSFFGVEQANWDNFTLEGGVRV
                     EKQKASIRYDKALIDRENYYNHPLPDLGAHRQTARSFALSGNWYFTPQHKLSLTASHQ
                     ERLPSTQELYAHGKHVATNTFEVGNKHLNKERSNNIELALGYEGDRWQYNLALYRNRF
                     GNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQSGADFYGAEGEIYFKPTPRYRIGVSGD
                     YVRGRLKNLPSLPGREDAYGNRPFIAQDDQNAPRVPAARLGVHLKASLTDRIDANLDY
                     YRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEWNWYVKADNLLNQSVYAHSSFL
                     SDTPQMGRSFTGGVNVKF"
BASE COUNT         1502 a         1642 c         1340 g         1371 t
ORIGIN      
        1 gaaaagtcgt gattcccagc cttcttttgc gacgccgcaa ccggtgccgg tcagttcgaa
       61 aaggatggta ttgttttgtc cgccaaaatg gacgcgaccg tataggacgt cttccgaacc
      121 catcaaccaa cagcgctcat agaaacgacc gcccgagcct ttggattctt tgtagatacc
      181 gaaaccgaaa acttcttcgg cgagcattga cgcggcgcga ataaaatctt cgtcttccaa
      241 aagacttaca cgaacgccgt atttatcgaa aaagggtttt tcatgaaatg aaaagctaat
      301 ttgatcaatg aaagccgaat ctgatacacc gcgccgaaga ggaacgccta acaggtttcc
      361 tttaccgtcc gttatgtacg tttcgtaaca ttcgaagact tcctgaaccc tgcctgccgt
      421 ttcggtttct gtaccccccc tgttaccccc cctgttagat aaggggggaa gatttgaagc
      481 ggttgccggc ttcctgccgt ccgctagcgc gtccgtcatc acgccggcaa ccgcctttgt
      541 catcccttgc ttatcttcca tggtgcgaat cctcaaaaac gggcaaaaaa aagccctgtt
      601 acttgtagaa agtaaaggac gttaattttt gttaatcgtc ccttcttagg gacgcaatat
      661 ataaggttta taccgtcgtt gttcctaatg cgcaatcagc gacattgttg ccaattatcc
      721 gaaagaaagt taagcctgat ggcattgtat acacggatac ctttcgtagt tatgatgtgc
      781 ttgatattag tgaatttagc catttacgta agtttaacgg cattcccaaa gagcatttgg
      841 ggctgcattt aaagaaatgc caatggcatt ttaaatagaa tctcaaattc agattttaag
      901 acaattagtt aatgggaatt tggtctggtt atctagtaca gccccaagtt tttatataaa
      961 aacagtttgg taagttctct tcgtgaagtg cttatgtttt gccacaaaag ttccataatc
     1021 atggcggcat cacccaagct ccatctttcc aagaaaatgg catctttgtt gtggttgaaa
     1081 accgacaaac tcctcttttt tcgatttggc cctgcctgaa tcgatggctt cccgtgcggc
     1141 agatatgccg tctgaaagcg aagcagcgac atttccggca tacagggcgg cggcggtatt
     1201 aagcaatacg atatcgcgcg cagccccttc tttcccatcc agcacctcat tcattttcaa
     1261 caaagattcc tgagtattgg caactttgat ttcatccaaa ttgcggcggg tttcgatacc
     1321 gaaatcttct gggcggatgt cgtattcgct gatttttccg tctttgagtt cggcaacgcg
     1381 cgttttgccc gtcagtgtaa tttcatccaa gccgccctcc ccgcaaacga ccaaaacgtg
     1441 ttttgaacca agttgttgca agacccgcga caaaatgccg cacaaatcgg tgtggaacac
     1501 gcccaaaagc tggttcggcg cgcccgcagg attcgttaac ggacccaata tgttgaaaat
     1561 acttcggaaa ccgagcgaac ggcgtacagg ggcgacatgg cgcatggcac tgtggtgatt
     1621 gggcgcgaac ataaacccga tgccggtctg cctgatactt tgggcaacct gttcgggagt
     1681 caggttgagg tttgcgccca tctgctccac cacgtcagcc gcaccgctgg aggaagagac
     1741 cgaccggcct ccgtgtttgg caaccttcgc gcctgccgct gcggcaacaa acatcgaagt
     1801 cgtcgaaata ttgaaggttt tcgcgccatc cccgcccgta ccgacgatat cgaccagccc
     1861 ctctgcattc tccagcggca cttttgtcgc aaactcgcgc atgacggctg cagctgcggt
     1921 aatttcggaa acggtttcaa ccttgatacg caatcctgtc aaaatggccg ctatctgctc
     1981 cggcagaacc tgtcctctca taatctgacg catcaagtcg gtcatttcat cgtaaaacaa
     2041 ctcgttattg ctgattaatc gttcgatggc ctgttgcggt gtaatcattt tttgtcctcc
     2101 gttcaatatt cggacgaaaa tgccgtctga agggcttcag acggcatcac gtcagatttt
     2161 ttgcggtttg aagttttgaa attcgattaa aaaattgttt aacatatcat gtccgtgctc
     2221 ggtcaagagg gcttcggggt ggaactgcac gccctcgacg gcatattcct tatggcgcac
     2281 acccataatc tcgccgtcct cagtccaagc cgttacttcc aaacattcgg gcatcgtatt
     2341 ccgatcgata acgaggctgt gataacgcgt acaggtaacc ggattgggca aacccttaaa
     2401 catacccttg cccgaatggg acacgggcga caccttgccg tgcatcagcg ttttggcgcg
     2461 gactatcctg ccgccgaacg cctcgcctat cgtctgatgc ccgaggcaca cgcccataat
     2521 cggcagccgg ccggcgaaat ggcgcatcgc cgccacggaa atccccgctt ctttgggcga
     2581 acacggaccg gggccgataa cgagatattg cggattcaat gcctcgattt cctccaacgt
     2641 aatatcatcg ttgcggcgca cggcaacttc ctgccccaat tcagtgaaat actggacgat
     2701 gttgtaagta aaactgtcgt aattgtcgat aaacaaaagc attttgtgat taacctattg
     2761 atttataatt tgtttgttat taactgcatt tccgataccc ccatccatac ccccttatat
     2821 cttagcgtgc ccgatgcgcc ctcgtgaatc tggcgcagag cctcgcgttc tgacgagtac
     2881 gccattttgc caccttttcg accgttcggg aacgtaccgt ttttatgcac tcataaattt
     2941 tggttatccc gcaaccgaac atagtgggca aacatagcgg aatctgcgtc aacatcaacc
     3001 gcgcccccga atgacggcgg atacaaatgg acaccgcccg atgccgcaac cgtttcattt
     3061 gaaaaacttt tgcctatgcc ctctgagtgt tctcaaaatc ttatgatata aaaaatgtaa
     3121 tgttatataa taacaaactt ttgaaatacg acgggaagcc aaaatggcac aaaccacact
     3181 caaacctatt gttttatcaa ttcttttaat caacacaccc ctcctcgccc aagcgcatga
     3241 aaccgatcgg tcggtggatt tggaaacggt cagcgtcgtc ggcaaaagcc gtccgcgcgc
     3301 gacttcgggg ctgctgcaca cttcgaccgc ctccgacaaa atcatcagcg gcgacacctt
     3361 gcgacaaaaa gccgtcaact tgggcgatgc tttggacggc gtgccgggca ttcacgcctc
     3421 gcaatacggc ggcggcgcgt ccgctcccgt tattcgcggt caaacaggca ggcggattaa
     3481 agtgttgaac catcacggcg aaacaggcga tatggcggat ttttcgcccg atcacgccat
     3541 tatggtagat accgccttgt cgcaacaggt cgaaatcctg cgcgggccgg ttacgctctt
     3601 gtacagctcg ggcaatgtgg cggggctggt cgatgttgcc gatggcaaaa tccccgaaaa
     3661 aatgcctgaa aacggcgtat cgggcgaact cggattgcgt ttgagcagcg gcaatctgga
     3721 aaaactcacg tccggcggca tcaatatcgg tttgggcaaa aactttgtat tgcacacgga
     3781 aggcttgtac cgcaaatcgg gcgattacgc cgtaccgcgt taccgcaatc tgaaacgcct
     3841 gcccgacagc cacgccgatt cgcaaacggg cagcatcggg ctgtcttggg ttggcgaaaa
     3901 aggctttatc ggcgcagcat acagcgaccg tcgcgaccaa tatggtctgc ctgcccacag
     3961 ccacgaatac gatgattgcc acgccgacat catctggcaa aagagtttga ttaacaaacg
     4021 ctatttgcag ctttatccgc acctgttgac cgaagaagac atcgattacg acaatccggg
     4081 cttgagctgc ggctttcacg acgacgatga tgcacacgcc catgcccaca acggcaaacc
     4141 ttggatagac ctgcgcaaca aacgctacga actccgcgcc gaatggaagc aaccgttccc
     4201 cggttttgaa gccctgcgcg tacacctgaa ccgcaacgac taccgccacg acgaaaaagc
     4261 aggcgatgca gtagaaaact tttttaacaa ccaaacgcaa aacgcccgta tcgagttgcg
     4321 ccaccaaccc ataggccgtc tgaaaggcag ctggggcgtg caatatttac aacaaaaatc
     4381 cagtgcttta tctgccacat ccgaagcggt caaacaaccg atgctgcttg acaataaagt
     4441 gcaacattac agctttttcg gtgtagaaca ggcaaactgg gacaacttca cgcttgaagg
     4501 cggcgtacgc gtggaaaaac aaaaagcctc catccgctac gacaaagcat tgattgatcg
     4561 ggaaaactac tacaaccatc ccctgcccga cctcggcgcg caccgccaaa ccgcccgctc
     4621 attcgcactt tcgggcaact ggtatttcac gccacaacac aaactcagcc tgaccgcctc
     4681 ccatcaggaa cgcctgccgt caacgcaaga gctgtacgca cacggcaaac acgtcgccac
     4741 caacaccttt gaagtcggca acaaacacct caacaaagag cgttccaaca atatcgaact
     4801 cgcgctgggc tacgaaggcg accgctggca atacaatctg gcactctacc gcaaccgctt
     4861 cggcaactac atttacgccc aaaccttaaa cgacggacgc ggccccaaat ccatcgaaga
     4921 cgacagcgaa atgaagctcg tgcgctacaa ccaatccggt gcggacttct acggcgcaga
     4981 aggcgaaatc tacttcaaac cgacaccgcg ctaccgcatc ggcgtttccg gcgactatgt
     5041 acgaggccgt ctgaaaaacc tgccgtccct acccggcagg gaagatgcct acggcaaccg
     5101 tcctttcatc gcacaggacg accaaaatgc ccctcgcgtt ccggctgcgc gcctcggcgt
     5161 ccacctgaaa gcctcgctga ccgaccgcat cgatgccaat ttagactact accgcgtgtt
     5221 cgcccaaaac aaactcgccc gctacgaaac acgcacgccc ggacaccata tgctcaacct
     5281 cggcgcaaac taccgccgca atacgcgcta tggcgagtgg aattggtacg tcaaagccga
     5341 caacctgctc aaccaatccg tttacgccca cagcagcttt ctctctgata cgccgcaaat
     5401 gggccgcagc tttaccggcg gcgtgaacgt gaagttttaa aatcggacag gcaaacaaaa
     5461 aaggttttca gacggcattc tgaaaacctt ttttcttttt aatcaatttg ttaggaaata
     5521 tggcaaagac aaagaacaaa ccactatgcc atcattccca ttttctttgt ttcatcattc
     5581 ttgctttcac gccgtcattc tcgcaaaagc agaaaaatca aaagcagaaa cccatcattc
     5641 ccgcgcaggc gagaatccaa ttcattcggt ttcagccgtt tccgataaat cgtcgtaacg
     5701 ttaagttccc ggattcccac tttcgtggga atgacggcgt aaaggtttct gttttttaag
     5761 ttttaggtaa ctaatgtgcc atcattcccg cgcaggcggg aatccaattc gttcggtttc
     5821 agccgtttcc gataaatcgc cttagcattt gggtt
//
LOCUS       DAXHSF010000128         5827 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.128, whole genome shotgun sequence.
ACCESSION   DAXHSF010000128 DAXHSF010000000
VERSION     DAXHSF010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5827
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.128"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..208
                     /locus_tag="ACFM8S_001623"
     CDS             <1..208
                     /locus_tag="ACFM8S_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314675.1"
                     /translation="FSELYMFQQRTAHQAPHCVLPERDYPPIRFYRHKQTGFNRKGMG
                     IKSISDISRAMPSENQSPLSDGIV"
     gene            285..1232
                     /locus_tag="ACFM8S_001624"
     CDS             285..1232
                     /locus_tag="ACFM8S_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFC6314676.1"
                     /translation="MNILSKISSFIGKTFSLWVALFAAAAFFAPDTFKWAGPYIPWLL
                     GIIMFGMGLTLKLSDFDILFKHPKAVIIGVIAQFAIMPATAWLLSKLLNLPAEIAVGV
                     ILVGCCPGGTASNVMAYLARGNVALSVAVTSVSTLISPLLTPAIFLMLAGEMLEIQAA
                     GMLMSIVKMVLLPIVLGLIVHKVLGSKTEKLTDALPLVSVAAIVLIIGAVVGASKGKI
                     MESGLLIFAVVVLHNGIGYLLGFFAAKWTGLPYDAQKTLAIEVGMQNSGLAAALAAAH
                     FAAAPVVAVPGALFSVWHNISGSLLATYWAAKAGKHKKP"
     gene            complement(1385..2509)
                     /gene="rluD"
                     /locus_tag="ACFM8S_001625"
     CDS             complement(1385..2509)
                     /gene="rluD"
                     /locus_tag="ACFM8S_001625"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFC6314677.1"
                     /translation="MQNTSFDNESDYSDDSDFVSAPETENCICLTVPLELAGGRLDAV
                     LAKLLPDYSRSRLTSWIKEGAVIVNDKPSQPKDKMIGGEQICVTVRPSEENLAFVPEP
                     MALDIIYEDDTVIVVNKPAGLVVHPAAGNWTGTLLNGLLAHCPELSQVPRAGIVHRLD
                     KETSGLMVVAKTLPAQNSLVQQLQERTVKRIYRAVANGIVPFDGKIETQIGRDPHNRL
                     KMAVVKFGGKPAVTHVKVLERYLAHSYIECSLETGRTHQIRVHMREANHPLAADPVYG
                     NPRHPCGDTVKEAVKSLGARQALHAYRLSFTHPESGETVSFEAPIPDDIYHLLSVLRL
                     EAGLDSSLSNEEEWQDKFGADDDDDWNEDDYDVEVVYVRE"
     gene            2508..3311
                     /locus_tag="ACFM8S_001626"
     CDS             2508..3311
                     /locus_tag="ACFM8S_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFC6314678.1"
                     /translation="MKKILLTVSLGLALSACATQGTVDKDAQITQDWSVEKLYAEAQD
                     ELNSSNYTRAVKLYEILESRFPTSRHARQSQLDTAYAYYKDDEKDKALAAIERFRRLH
                     PQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
                     KYAADATARMVKLVDALGGNEMSVARYYMKRGAYIAAANRAQKIIGSYQNTRYVEESL
                     AILELAYQKLGKPQLAADTRRVLETNFPKSPFLTHAWQPDDMPWWRYWH"
     gene            complement(3369..5588)
                     /locus_tag="ACFM8S_001627"
     CDS             complement(3369..5588)
                     /locus_tag="ACFM8S_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFC6314679.1"
                     /translation="MFRRWFLPCWVVGAAASFALPVVPHWLFWLAAFAVFAVFARRFA
                     FAGLMLCVLAGAAYGVFRTEAALSSQWWAEAVSGVPLTVEVADMPRSDGRRVQFAAKA
                     VDSGGRTFDLLLSDYKRREWAVGSRWRITARVHPVVGELNLRGLNREAWALSNGIGGA
                     GTVGADRVLLHGGSGWGIAVWRSRISRNWQQADADGGLSDGIGLMRALSVGEQSALRP
                     ELWQAFRPLGLTHLVSISGLHVTMVAVMFAWLAKRLLACSPRLPARPRAWVLAAGCAG
                     ALFYALLAGFSVPTQRSVLMLAAFAWAWRRGRLSAWATWWQALAAVLLFDPSAVLGVG
                     TWLSFGLVAALIWACSGRLHEGKRQTALRGQWAASVLSLVLLGYLFASLPLISPLVNA
                     VAIPWFSWVLTPLALLGSVVPFAPLQQLGAFLAEYTLRFLVWLADVSPEFAVASAPLP
                     LLVLAVCAALLLLLPRGLGLRPWAVLLLAGFVFYRSPGVPENEVAVTVWDAGQGLSVS
                     VQTANHHLLFDTGTAAAAQMGIVPSLNASGIRYLDYLVLSHHDSDHDGGFQAVGKIPN
                     GGIYAGQPEFYEGARHCAEQRWQWDGVDFEFLMPSERKNIDDNGKSCVLRVVAGGAAL
                     LVTGDLDTKGEESLVGKYGGNLYSQVLVLGHHGSNTSSSGVFLNAVSPEYAVASSGYA
                     NAYKHPTEAVQNRVRAHGIKLLRTDLSGALQFGLGRGGVKAQRLRGYKFYWQKKPFE"
BASE COUNT         1426 a         1824 c         1401 g         1176 t
ORIGIN      
        1 tttctccgag ctttatatgt ttcaacagag gacggcacat caagcaccgc actgcgtgtt
       61 gcccgaacga gactaccctc cgattagatt ctatcgccat aaacagacgg gtttcaaccg
      121 aaaaggaatg gggataaagt ccatttccga catctctcgg gcgatgccgt ctgaaaacca
      181 atctccactt tcagacggca ttgtttaggc acggtcattt tgcaaattaa ggttaaggcg
      241 tgtatgcttg ccttccctta aaaacaaaca cgcaacgcat tattatgaat atcctcagta
      301 aaatcagcag ctttatcgga aaaacatttt ccctctgggt cgcgctcttt gccgccgccg
      361 cttttttcgc gcccgacacc ttcaaatggg cggggcctta tattccttgg ctgttgggca
      421 ttattatgtt cggtatgggt ttgacgctca aactttccga cttcgatatt ttgttcaaac
      481 atcccaaagc cgtcatcatc ggcgtaatcg cacaattcgc cattatgccg gcaaccgcct
      541 ggctgctgtc caaactgttg aacctgcctg ccgaaatcgc ggtcggcgtg attttggtcg
      601 gctgctgccc gggcggtacg gcttccaatg tgatggccta tctggcgcgc ggcaatgtgg
      661 ctttgtcggt tgccgttacg tctgtttcca ccctgatttc cccattgctg actcccgcca
      721 tcttcctgat gcttgccggc gaaatgctgg aaatccaagc ggccggtatg ttgatgtcca
      781 tcgtcaaaat ggttttgctc cccattgttt tgggtttgat tgtccataag gttttgggca
      841 gtaaaaccga aaagctgacc gatgcgctgc cgctggtttc cgttgccgcc atcgtgctga
      901 ttatcggcgc ggttgttggg gcaagcaaag gcaagattat ggaaagcggc ctgctgattt
      961 ttgcggttgt cgtactccac aacggcatcg gctacctgct cggcttcttt gccgccaaat
     1021 ggaccggcct gccttatgat gcacaaaaaa cgctggccat cgaagtcggt atgcaaaact
     1081 cgggcctggc cgccgcgctt gccgccgcac actttgccgc cgcgccggtc gttgccgttc
     1141 cgggcgcatt gttcagcgtg tggcacaata tctccggctc gctgctggca acttattggg
     1201 cggccaaagc cggtaaacat aaaaaaccct aaacgccttt catcataatg ccgtctgaac
     1261 ggggcaaacg aaaccggcgg tttctgcccg gccccttcag acggcctttg tttgaaatcg
     1321 gcggggacga ttgcccggct gctccggttc ggctgcctgc gttcgccccg ccttgcaaac
     1381 cgcctcactc ccttacataa accacttcga catcgtagtc gtcttcgttc caatcgtcat
     1441 cgtcgtccgc gccgaatttg tcctgccatt cctcttcatt gctcaaagac gaatccaaac
     1501 cggcttcaag acggaggaca gacaataaat gatatatgtc gtccggaatc ggtgcctcaa
     1561 acgaaacggt ttcgccgctt tccggatggg taaaactcaa gcggtaggcg tgcaacgcct
     1621 gacgcgcgcc caaactttta acggcttctt tcaccgtgtc gccgcacgga tggcgcgggt
     1681 tgccgtaaac cgggtcggcg gcaagcggat ggttggcctc acgcatatgg acgcggattt
     1741 ggtgagtcct gcctgtttcg agcgagcatt cgatatagct gtgcgccaga tagcgttcca
     1801 acactttgac gtgggtaacg gctggtttgc cgccgaattt gacgactgcc attttcaggc
     1861 ggttgtgcgg atcgcgtccg atttgggttt cgattttacc gtcaaaggga acgatgccgt
     1921 tggcgacggc gcggtaaatg cgtttgaccg tgcgctcttg aagctgttgc acgagggaat
     1981 tttgcgccgg cagggttttg gcaaccacca tcagcccgct ggtttctttg tccaaacggt
     2041 gtacgatgcc tgcacgaggt acttggctca attcgggaca atgcgccaag aggccgttga
     2101 gcagcgttcc cgtccagttg ccagccgccg gatgcaccac cagtccggcc ggtttgttga
     2161 caacgatgac ggtatcgtct tcgtaaataa tatccaaagc cataggctct ggaacaaacg
     2221 ccagattttc ctcactcgga cggacggtta cacaaatttg ctcgccgcct atcattttgt
     2281 ctttgggttg cgaaggttta tcgtttacaa taaccgcgcc ttctttaatc catgatgtca
     2341 ggcggctgcg cgagtagtcg ggcagaagtt tcgccaacac cgcatccagc cgcccgccgg
     2401 caagctcgag cggaacagtc agacaaatac agttttcggt ttccggggct gacacaaagt
     2461 ctgaatcatc gctataatcg gattcattat caaaggaagt attctgcatg aaaaaaattc
     2521 ttttaacggt ttcattaggt ttggcgctga gtgcctgtgc cactcaaggt acggtagata
     2581 aagatgccca gattacccaa gactggagtg tggaaaaact ctatgccgaa gcccaggatg
     2641 agctgaacag cagcaattat actcgagctg tcaaattata cgaaatcttg gaatcgcgct
     2701 tccccaccag ccgccatgcc cggcagtcgc aactggatac cgcatacgcc tattataaag
     2761 acgatgaaaa agacaaagct ctggcggcaa tcgaacgctt ccgccgcctc catccgcagc
     2821 atccgaatat ggattacgcg ctgtatctgc gcggcttggt gctgttcaac gaagaccagt
     2881 ccttcttgaa caaactggcc tcgcaagact ggtccgaccg cgacccgaaa gccaaccgcg
     2941 aagcgtacca ggcgtttgcg gaactcgtcc aacgtttccc caacagcaaa tacgccgccg
     3001 atgcgaccgc acgcatggtc aaactggtcg atgcactggg cggcaatgaa atgtcggtgg
     3061 cgcgctacta catgaaacgc ggtgcataca tcgccgccgc caaccgcgcc caaaaaatta
     3121 tcggcagcta tcaaaatacg cgttatgttg aagaatcgct cgccatattg gaacttgcct
     3181 accaaaaact cggcaaacca cagcttgccg ccgatacgcg ccgcgtgttg gaaaccaact
     3241 tcccgaaaag cccgtttttg acgcacgctt ggcagcccga cgatatgccc tggtggcgtt
     3301 actggcatta agcccatctt cgtaatgccg tctgaagccg tttcagacgg cattttatgt
     3361 ttcaaacctc attcaaacgg ttttttctgc caatagaatt tataccctct caaacgttga
     3421 gccttcacgc cgccgcgtcc caagccgaat tgcagtgcgc ccgacaaatc ggtacgcagc
     3481 agtttgatgc cgtgtgcgcg gacacggttc tgcaccgctt cggtcggatg tttgtaggcg
     3541 ttggcataac cgcttgaagc aacggcatat tcgggcgaga cggcattgag gaacacgccc
     3601 gacgaggacg tattgctgcc gtgatgtccc aaaaccaaaa cctggctgta caggttgcct
     3661 ccatacttgc cgaccaggct ttcctcgccc ttcgtatcca aatcgcccgt taccagcagt
     3721 gccgcaccgc ccgccacaac acgcaaaaca caacttttcc cattatcatc gatgtttttg
     3781 cgttcagacg gcatcaaaaa ctcaaaatcc acgccgtccc attgccaacg ctgttccgca
     3841 caatgccgcg ccccctcata aaattccggc tgcccggcat aaatcccgcc gttcggtatc
     3901 ttgccgactg cctgaaaacc gccgtcgtgg tcgctgtcgt gatgcgacaa aaccaaataa
     3961 tccaaatacc tgattcccga cgcattcaag ctcggcacaa tccccatctg cgccgccgct
     4021 gccgttccgg tgtcaaacaa aagatgatga tttgccgtct gaaccgacac cgacaaaccc
     4081 tgccccgcat cccaaaccgt aaccgcaacc tcattttccg gcacgccggg tgaacggtaa
     4141 aacacaaacc ctgccaacag caacaccgcc cacggacgca aacccaagcc gcgcggcagc
     4201 agcaacagca aagcggcaca caccgccaac accaacagcg gcaaaggtgc ggaggcaacg
     4261 gcaaactcgg gcgacacatc ggcaagccac accaaaaacc gcaaagtata ttccgccaaa
     4321 aatgccccca actgttgcaa aggcgcaaac ggcacgaccg aacccagcaa cgccagcggc
     4381 gtcaataccc aagaaaacca cggaattgcc accgcattga ccaaagggct gattaaaggc
     4441 agcgaagcaa acagataacc gagcaaaacc agcgacaaca ccgaagccgc ccactgcccg
     4501 cgcaaagcag tttgccgttt tccctcgtgc aaacgccccg agcacgccca tatcagggcc
     4561 gccaccaaac cgaaagacag ccaagtcccc acacccaaga ccgccgacgg gtcgaacagc
     4621 agcaccgccg ccaacgcctg ccaccacgtc gcccacgccg acaatcttcc cctgcgccaa
     4681 gcccacgcaa acgccgccaa catcaaaacg ctgcgctgcg tcggcacgga aaaaccggca
     4741 agcagcgcgt aaaacagcgc gcctgcacac cccgccgcca aaacccacgc gcgcggccgg
     4801 gcaggaaggc gcggggaaca ggcaagcagc cgcttcgcca gccacgcaaa cattaccgcc
     4861 accatcgtaa cgtgcaaacc cgaaatactg accaaatgcg tcagccccaa cggccggaac
     4921 gcctgccaca attcggggcg caatgccgac tgttcgccca cgctcaacgc gcgcatcagc
     4981 ccgatgccgt ctgaaagccc gccgtccgca tccgcctgct gccaattacg gctgatgcgg
     5041 ctgcgccaaa ccgcaatccc ccaaccgctt ccgccatgca gcaaaaccct gtccgcaccg
     5101 accgtccccg cgccgcctat tccgttggat aatgcccacg cctcacggtt caaaccgcgc
     5161 aggttcaact ccccgacgac agggtgcaca cgtgccgtta tccgccatct gctcccgacc
     5221 gcccattcgc gccgtttgta gtccgacagc agcaaatcaa acgtccgacc accgctgtcc
     5281 acagcctttg ccgcaaactg cacacgccgc ccgtccgacc tcggcatatc cgccacttcc
     5341 accgtcaaag gcacgcctga aacggcttct gcccaccatt gggaagacaa tgccgcctcc
     5401 gtcctgaata cgccgtaagc cgcgcccgcc aacacgcaca gcatcagacc ggcaaacgca
     5461 aaacgccttg caaacacagc aaaaaccgca aaagccgcca gccaaaacag ccaatgcggc
     5521 acgaccggca gcgcaaacga agccgccgca ccgacaaccc aacacggcaa aaaccaacga
     5581 cggaacacgt ctccccaccc cagatatttc aaaaataaac ggataataaa gcaaatccga
     5641 aaaaccgtca taacaagatt tgaaaatgat atagtggttt aaatttaaat caggacaagg
     5701 cgacgaagcc gcagacagta caagcagtac gataaggcga ggcaacgctg tactggtttt
     5761 tgttaaacca ctataagaat gcaaacgaaa agcctcagta ttatccgaga caacggcaaa
     5821 ctttcca
//
LOCUS       DAXHSF010000129         5709 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.129, whole genome shotgun sequence.
ACCESSION   DAXHSF010000129 DAXHSF010000000
VERSION     DAXHSF010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5709
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.129"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(129..890)
                     /locus_tag="ACFM8S_001628"
     CDS             complement(129..890)
                     /locus_tag="ACFM8S_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314680.1"
                     /translation="MDANKEPNWEKRLENLRQTRRMKAVPTYAPLSRPSETGTDAPVP
                     KLDDESRARVLKAYLKKWQEEHNAAVADCGEEVETDPMIVLQENWLNAQASLQMSVSE
                     KHIESRRRILFDAKKDSAEFETGQIEGGENADAAESGTAADGVAENGEGGAEEALMPV
                     QINILNPKAVNRREVFCLSEQELTERLIKRLRPHLTDTVNGIIRTAVQKQMAVFTYQL
                     QQMLHEQAGAVVEDVLEHDVRKILNDIKYELKYKR"
     gene            989..1240
                     /locus_tag="ACFM8S_001629"
     CDS             989..1240
                     /locus_tag="ACFM8S_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314681.1"
                     /translation="MGSIEQRLEYLEEANDVLRMQNHVLSTAFKALIRALPAETAEIA
                     VESIQLAFEDALAELSYEDSPHTDLFHDVTYAFFREKER"
     gene            1292..2098
                     /locus_tag="ACFM8S_001630"
     CDS             1292..2098
                     /locus_tag="ACFM8S_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HFC6314682.1"
                     /translation="MNMKKWIAAALACSALALSACGGQGKDAAAPAANPDKVYRVASN
                     AEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMS
                     GVTITDDRKQSMDFSDPYFEITQVVLVPKGKKISSSDDLKNMNKVGVVTGYTGDFSVS
                     KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPTKGMDFVTLPDF
                     TTEHYGIAVRKGDEATVKMLNDALEKVRESGEYDKIYAKYFAKEDGQAAK"
     gene            2405..3928
                     /locus_tag="ACFM8S_001631"
                     /pseudo
     CDS             2405..3928
                     /locus_tag="ACFM8S_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221888.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
     gene            4026..5438
                     /gene="trkA"
                     /locus_tag="ACFM8S_001632"
     CDS             4026..5438
                     /gene="trkA"
                     /locus_tag="ACFM8S_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFC6314683.1"
                     /translation="MKILILGNGQVGSTVAQNLAAIPNNDVTVIDIDEKALQETGSRL
                     DVQTVFGNGASPFTLERAGAEDADLLLALSRSDETNIVACKVAADLFNIPGRIARVRS
                     SEYLEYLSPKLENNENGSLSIFGITETISPEQLVTEQLAGLIDCPGALQVLRFADDRV
                     RMVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEIL
                     FAAAAENIGAVIPELRPKETSTRRIMIAGGGNIGYRLAKQLEHAYNVKIIECRPRRAE
                     WIAENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRV
                     IGIVNRSSYVDLLEGNKIDIVVSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEVV
                     AHGDKKTSAIIGRRISGIKWPEGCHIAAVVRAGTGETIMGHHTETVIQDGDHIIFFVS
                     RRRILNELEKLIQVKMGFFG"
BASE COUNT         1433 a         1708 c         1382 g         1186 t
ORIGIN      
        1 cggcttcgtc gccttgtcct gatttaaatt taatccacta tattaatatt ttgcgcctgt
       61 tctatgatgc tttcaagtcg ggtgagaatg caaatgccgt ctgaaacggc tttcagacgg
      121 catggcaatc agcgtttgta ttttaactcg tacttgatgt cgttgaggat tttgcggaca
      181 tcgtgttcca acacgtcttc gactaccgca cccgcctgct cgtgcagcat ctgctggagc
      241 tgataggtaa aaaccgccat ctgcttttgc accgccgtac ggatgatgcc gttgacggta
      301 tcggtcagat gcgggcgcag gcgtttgatc agccgttcgg tcagctcctg ttcggacagg
      361 cagaacactt cgcgccggtt gacggctttc ggattcagga tattgatttg gacgggcatc
      421 aacgcctctt ccgcaccgcc ttcgccgttt tccgcaaccc cgtctgccgc cgtacccgat
      481 tctgccgcat cggcgttttc cccgccttca atctgtccgg tttcaaattc ggcactgtct
      541 tttttggcat cgaacaggat tctccgccgc gattcgatgt gtttttccga aaccgacatt
      601 tgcagggaag cctgcgcgtt gagccagttt tcctgaagga caatcatcgg gtcggtttcg
      661 acttcctcgc cgcaatcggc aacggcggca ttgtgttcct cctgccattt tttcagatac
      721 gccttcaaca cacgggctcg gctctcatcg tccagtttcg gcacaggcgc gtccgttccg
      781 gtttcagagg ggcgggacag cggcgcgtaa gtcggcactg ccttcatacg gcgcgtctga
      841 cgcaggtttt ccaaacgttt ttcccaattc ggctctttat tcgcatccat tttcggcttc
      901 cggttcttaa tctttgcaag cagacaaacc cgcgcccaaa gcgcgtttga tataatcgcg
      961 cattttaaca gattcgcgag gatacatcat gggcagcatc gaacagcgtt tggaatatct
     1021 ggaagaggcg aacgacgtgc tgcgtatgca gaaccacgtc ctgtccaccg cattcaaagc
     1081 cttaatccgc gcccttcccg ccgaaaccgc cgaaatcgcg gtcgagtcga ttcagcttgc
     1141 ttttgaggac gccttggcag aattgagcta tgaggacagc ccccataccg atttgttcca
     1201 cgacgttact tatgcgtttt tccgtgaaaa agaacgttaa ttttatgtta aactgatttt
     1261 ttaggctttt tgattaccga aaggaatttt gatgaatatg aaaaaatgga ttgccgccgc
     1321 ccttgcctgt tccgcgcttg cgctgtctgc ctgcggcggt cagggtaaag atgccgccgc
     1381 gcccgccgca aatcccgaca aagtgtaccg cgtggcttcc aacgccgagt ttgccccctt
     1441 tgaatcttta gactcgaaag gcaatgtcga aggtttcgat gtggatttga tgaacgcgat
     1501 ggcgaaggcg ggcaatttta aaatcgaatt caaacaccag ccgtgggaca gccttttccc
     1561 cgccttgaac aacggcgatg cggacgttgt gatgtcgggc gtaaccatta ccgacgaccg
     1621 caaacagtct atggacttca gcgacccgta ttttgaaatc acccaagtcg tcctcgttcc
     1681 gaaaggcaaa aaaatatctt cttccgacga tttgaaaaac atgaacaaag tcggcgtggt
     1741 aaccggctac acgggcgatt tctccgtttc caaactcttg ggcaacgaca atccgaaaat
     1801 cgcgcgcttt gaaaacgttc ccctgattat caaagaactg gaaaacggcg gcttggattc
     1861 cgtggtcagc gacagcgcag tcatcgccaa ttatgtgaaa aacaatccga ccaaagggat
     1921 ggacttcgtt accctgcccg acttcaccac cgaacactac ggcatcgcgg tacgcaaagg
     1981 cgacgaagca accgtcaaaa tgctgaacga tgcgttggaa aaagtacgcg aaagcggcga
     2041 atacgacaag atttacgcca aatattttgc aaaagaagac ggacaggccg caaaataagc
     2101 ccgcccgtcc gaacacaatg ccgtctgaag ccttttcaga cggcattgtt catcaaccgg
     2161 cctacaatga actgcctgct gatttctccc taccgcaaag caacaggcaa agattacaaa
     2221 tatcaaaatc cgagtaaaac agtattttat taaaacaaat tgataatcaa gagattagaa
     2281 ttatgtattg tctttaccgt acaaacgctg gcactatttc aacctgataa aaaacagcct
     2341 tcaaaaaggt tgcttaaaac agcagcagac acttaccgcc acaaccttga aaaggaacac
     2401 aatcatgacc gtcatcaagc aagaagactt tatccaaagt atctgcgatg ccttccaatt
     2461 catcagctac taccatccga aagactacat cgacgcgctt tataaggcgt gacagaagga
     2521 agaaaatccc gccgccaaag acgcgatgac gcagattttg gtcaacagcc gtatgtgtgc
     2581 ggaaaacaac cgccccatct gccaagatac cggtatcgca accgtgtttt tgaaagtcgg
     2641 tatggatgtg cagtgggatg cggatatgag cgtggaagaa atggttaacg aaggcgtgcg
     2701 ccgcgcctac acttgggaag gcaatacgct gcgcgcttcc gttctcgccg accccgccgg
     2761 caaacgccaa aacaccaaag acaacacccc cgccgtcatc catatgagca tcgtgccggg
     2821 cggtaaagtc gaagtaacct gcgcggcaaa aggcggcggc tctgaaaaca aatccaaact
     2881 cgccatgctc aatccttccg acaacatcgt cgattgggta ttgaaaacca tcccgacgat
     2941 gggcgcgggc tggtgtcctc ccggcatctt gggcatcggc atcggcggca cgcctgaaaa
     3001 agccgtgttg atggcaaaag aatccctgat gagccacatc gacattcaag agttgcagga
     3061 aaaggccgcg tccggcgcag aattgtccac caccgaagcc ctgcgcctcg aactctttga
     3121 aaaagtcaac gcgctgggca tcggcgcaca aggtttgggc ggtctgacca ccgtgttgga
     3181 cgtgaaaatc ctcgattacc cgacccacgc cgcctccaaa ccgattgcca tgattccgaa
     3241 ctgcgccgcc acccgccacg tcgaatttga attggacggc tcaggccctg tcgaactcac
     3301 gccgccgcgc gtcgaagact ggcccgattt gacttacagc cccgacaacg gcaaacgcgt
     3361 cgatgtcgat aagctgacca aagaagaagt ggcaagctgg aaaaccggcg acgtattgct
     3421 gctgaacggt aaaatcctca ccggccgcga tgccgcacac aaacgcctcg tcgatatgct
     3481 caacaaaggc gaagaattgc ccgtcgattt caccaaccgc ctgatttact acgtcggtcc
     3541 cgtcgatccg gtcggcgatg aagtcgtcgg tccggcaggt ccgaccacag ccacccgcat
     3601 ggacaaattc acccgccaaa tgctcgaaca aaccgacctc ttgggcatga tcggcaaatc
     3661 cgagcgcggc gtggccacct gcgaagccat cgccgacaac aaagccgtgt acctcatggc
     3721 agtcggcggc gcggcgtatc tcgtggcaaa agccatcaaa tcttccaaag tcttggcgtt
     3781 ccccgaattg ggcatggaag ccatttacga atttgaagtc aaagacatgc ccgtaaccgt
     3841 cgccgtagac agcaaaggcg aatccatcca cgccaccgcc ccgccccaat ggcaggcgaa
     3901 aatcggcatc atccccgtcg aatcttgagg cgtcatgccg tctgaacaca aaatctgctt
     3961 tcagacggca tttccgcccc cggttgcggt acaatccacc atttcatcac tcggcgaccc
     4021 acaccgtgaa aatcctcatt ttaggcaacg gacaggtagg ttctaccgtc gcacaaaacc
     4081 ttgccgccat acccaacaac gacgtaaccg ttatcgacat cgacgaaaaa gcattgcagg
     4141 aaacaggcag ccgcctcgat gtccaaaccg ttttcggcaa cggcgcatcc cccttcacat
     4201 tagaacgcgc cggcgcggaa gatgccgact tgctgctcgc gctctcccgc agcgacgaaa
     4261 ccaacatcgt cgcctgcaaa gttgccgccg acctgttcaa catccccggc cgcatcgcgc
     4321 gcgtccgttc cagcgaatac ctcgaatacc tcagccccaa gctcgaaaac aacgaaaacg
     4381 gcagcctttc catattcggc ataaccgaaa ccatcagccc cgaacagctc gttaccgaac
     4441 agcttgccgg cctgatagac tgcccgggcg cattgcaggt tttacgtttt gcagacgacc
     4501 gcgtgcggat ggtcatcata caggcgcggc gcggcggact gcttgtcgga cgcagcattg
     4561 ccgacatcgc ccaagatttg cccgacgggg ccgactgcca aatctgcgcc gtttaccgca
     4621 acaaccgcct catcgtcccc gcgccgcaaa ccgtcatcat cgaaggcgac gaaatcctat
     4681 ttgccgccgc cgccgaaaac atcggcgcgg tcatacccga attgcgcccc aaagaaacca
     4741 gcacccgccg catcatgatt gccggcggcg gcaacatcgg ctaccgtctc gccaagcagc
     4801 tcgaacacgc atacaacgtc aaaatcatcg aatgccggcc gcgccgtgcc gaatggatag
     4861 ccgaaaacct cgacaacacc ctcgtcctgc aaggttcggc aaccgacgaa accctgctcg
     4921 acaacgaata catcgacgaa atcgacgtat tctgcgccct gaccaacgac gacgaaagca
     4981 acattatgtc cgcccttttg gcgaaaaacc tcggcgcgaa gcgcgtcatc ggcatcgtca
     5041 accgctcaag ctacgtcgat ttgctcgaag gcaacaaaat cgacatcgtc gtctcccccc
     5101 acctcattac catcggctcg atactcgccc acatccggcg cggcgacatc gttgccgtcc
     5161 accccatccg gcgcggcacg gcggaagcca tcgaagtcgt cgcacacggc gacaaaaaaa
     5221 cttccgccat catcggcagg cgcatcagcg gcatcaaatg gcccgaaggc tgccacattg
     5281 ccgccgtcgt ccgcgccgga accggcgaaa ccattatggg acaccatacc gaaaccgtca
     5341 tccaagacgg cgatcacatc atctttttcg tctcgcgccg gcgcatcctg aacgaactgg
     5401 aaaaactcat ccaagtcaaa atgggctttt tcggataaac cgccccattc cggacacatt
     5461 gccgccaagc ggtatggaag cggaataatg gtaggtgggc ttcagacggc atccgccctc
     5521 cccgtcattc ccgcgtaagc gggaatccag accttgggat agcggcaata ttcaaaggtt
     5581 agctgaagct ttagagattc tagattcccg tttccacggg aatgacgaaa ggttgcggga
     5641 atccaaacca ttgggcaaca gcaatattca aagattcttg gattcggatt tcaagtgcaa
     5701 cactagtgt
//
LOCUS       DAXHSF010000130         5696 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.130, whole genome shotgun sequence.
ACCESSION   DAXHSF010000130 DAXHSF010000000
VERSION     DAXHSF010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5696
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.130"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            131..565
                     /locus_tag="ACFM8S_001633"
     CDS             131..565
                     /locus_tag="ACFM8S_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HFC6314684.1"
                     /translation="MPLPAPCRFAKPAASFLSMALLSCQLSHAATAYIPLNDFQPNCD
                     IRRLGLTQGQHNELRKIRAAFKMAGDRARLKVMHSEHSRRRSVVEIISSDVFNRNEAR
                     DYVESRYLSGMDFAVDELEIQHRFFHILTPQQQQMWLSSCLK"
     gene            complement(578..1443)
                     /locus_tag="ACFM8S_001634"
                     /pseudo
     CDS             complement(578..1443)
                     /locus_tag="ACFM8S_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241590.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(1451..2068)
                     /locus_tag="ACFM8S_001635"
     CDS             complement(1451..2068)
                     /locus_tag="ACFM8S_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFC6314685.1"
                     /translation="MFTGIVQGLGKLTAIHRPSEAFHTYVVELPQEAADNLQRGASVA
                     NNGCCLTITEIEGNRVSFDLMAETLAKTNLGLLKEGDCVNIERAARFGDEIGGHVMSG
                     HIMATVPIVEIERDGFNRTVWFALPHELKPYILTKGFVGLDGCSLTIGKVEDGRFNVH
                     LIPETLERTLFGSRKVGDRINIEIDPNTQAIVDTVERLMAQRYAK"
     gene            complement(2231..2845)
                     /locus_tag="ACFM8S_001636"
     CDS             complement(2231..2845)
                     /locus_tag="ACFM8S_001636"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HFC6314686.1"
                     /translation="MKTSNFSKRTSAMKTFALILAGGQASRMGGEDKGLALLGGKALI
                     DHVIDRVRPQVSHIAISTNRNLEEYARRSPHIFPDARQWQHFGPLSALCTAANDLQLA
                     TADWLLVVPCDMPYLPDDLVARFETVSKRTPLCNAYYVETPITMHYNIMYIRPQILQS
                     AIPYLFSGMKTLRSWLQQQRARPVRFEFDGHFADLNTQIDLQEG"
     gene            3051..4823
                     /locus_tag="ACFM8S_001637"
     CDS             3051..4823
                     /locus_tag="ACFM8S_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite sensor histidine kinase"
                     /protein_id="HFC6314687.1"
                     /translation="MILPARFSDGISLSLRLKLLTGLWVGLAALSVVLTLLLSLRLEN
                     AASVIEEAGNLRMQAYRLAYMAGEGSPRAQIDNQVAEFEKSLNRISQSDAIHPLIPSD
                     TPLAYDLIQSMLIIDWQAHILPPLQSYRRPTQVDLYRFAGNIELFLQALENANEKNTW
                     WLRRFQWAIMLMTLVSSVLMLFWHQIWVIRPLQALREGAERIGRRCFDIPVPEGGTPE
                     FKQVGRCFNQMGGRLKILYDDLEGQVAEQTRSLEKQNQNLTLLYQTTRDLHQSYIPQQ
                     AAEHFLNRILPAVGADSGRVCLDGGSDVYVSIHHADCGTAASDLGKYHEEIFPIEYQN
                     ETLGRLLLSFPNGISLDEDDRILLQTLGRQLGVSLAGAKQEEEKRLLAVLQERNLIAQ
                     GLHDSIAQALTFLNLQVQMLETAFAENKREEAAENIGFIKTGVQECYEDVRELLLNFR
                     TKISNKEFPEAVADLFARFTQQTGITVETAWENGSFLPPQEAQLQMIFILQESLSNIR
                     KHARATHVKFTLSEHGGRFTMTIQDNGQGFDTEKIGEPTGSHVGLHIMQERAKRIHAV
                     LEIRSQAQQGTTVSLTVASEESLK"
     gene            4820..5476
                     /locus_tag="ACFM8S_001638"
     CDS             4820..5476
                     /locus_tag="ACFM8S_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFC6314688.1"
                     /translation="MTIKIILIDDHTLFRSGIKALLSRQHGFEVIGEAADGLSGIKMI
                     SRLQPDVVLLDLDMPGMNGREALSQIISINPQQAVIMLTVSEDSDDLTECIRIGARGY
                     LLKNINADFLLESIRKAAEGDNVFSPEMTAKLVKSLISPQPAQGTQALSSLTPRELEI
                     LGYLAAGHSNKIIARHLDLAESTVKVHVQNLLRKLNLSSRVQAAVYAIRHNVPQPVLE
                     "
BASE COUNT         1390 a         1612 c         1336 g         1358 t
ORIGIN      
        1 ttccgattcc gccgttatgt ctgcctgccg gacatacgta cgcattatta acaaaagtta
       61 accgcgataa taccatcttt cacacgtcaa tctagtatat ttcctaaaat ttccaacaag
      121 aggaaaagcc gtgccactgc ctgctccctg ccgttttgcc aaacctgccg cctctttttt
      181 aagtatggct ttgctttcct gccagctttc ccacgccgcc acggcttata tccccctgaa
      241 cgattttcaa ccgaactgcg acatacgccg gctcggactg acacagggtc agcacaatga
      301 actgcgtaaa atccgcgccg ccttcaaaat ggcgggcgac agggcgcgtt tgaaggttat
      361 gcattccgaa cacagccgcc gccggtctgt cgtcgaaatc atttcttcgg atgtttttaa
      421 tcggaacgag gcgcgcgatt atgtcgaaag ccgctatttg tccggtatgg attttgcggt
      481 ggacgaattg gaaatccaac accgcttctt ccatatcctc acaccgcaac agcagcaaat
      541 gtggctttct tcctgcctca aataatcccc gaaacgctca cggcgcacgt tgctccggca
      601 gcctgcccgc ccagtcgcag gcaaactgcc acgcggaacg tcccgaacgg ctgccccgcg
      661 tctgcgccca ctgcaatgcc gccatctgtg cggtttcatc atacggcacg ccgaaatctt
      721 ccagccaatt ttgcaccgcc gccagataat cgttttgatc gaacggataa aaactgagcc
      781 acaatccgaa tcggtcggac agggatattt tttcttccac cgcttctttc tgatggactt
      841 ccccccccgc atccccgtcg taccggcatt ctcgtcaaaa tattcgggca tcaggtgccg
      901 tctgttggaa gtcgcgtaaa ccaaaacgtt ggcgcaacgt tgagacagac cgccgtctaa
      961 cgcggttttc aatgccttat aggtttcatc gccgctttca aacgacaaat cgtcgcaaaa
     1021 tacgataaat ttttccggac gttccttcaa aagcgtcaac aggtaaggca ggccgattaa
     1081 atcgctttta tcgacttcga tcaggcgcaa tcccttatcc gcatattcgt gtagcagggc
     1141 tttgaccagc gaggatttgc ctgttccgcg cgcgccgctc atcaatacgt tattcgcggg
     1201 tctgccgaca atgaactgtt cggtattacg caccagcaat tcggtttgcc tgccgactcc
     1261 cgccagcctt accaagggaa aggtgtgcgg atcgggcaag tgttccaaaa aacctttttt
     1321 gcccgcactc tgccagcgga aggcaagcgc gttccaatcc gtatgcccgg gttcgggcgg
     1381 aagcacggca tccaaacgcc gcaaaacggc ataggcttta tcgagaaatt cgttcaattc
     1441 catctctacc tcactttgca tatctttgcg ccatcagccg ttcgacggtg tcgacgattg
     1501 cctgcgtatt cggatcgatt tcgatgttga tcctgtcgcc gacctttctg ctgccgaaca
     1561 gcgtccgttc caaagtttcg ggaatcagat ggacattgaa acggccgtct tcgactttgc
     1621 ctatggtcag gctgcaaccg tccaagccga cgaagccttt ggtcaggata tagggtttga
     1681 gttcgtgcgg gagcgcaaac caaaccgtgc ggttgaaccc gtctcgttcg atttcgacaa
     1741 tgggcacggt tgccataatg tgcccgctca tgacgtgtcc gccgatttcg tcgccgaaac
     1801 gcgccgcccg ttcgatgttg acgcaatcgc cttccttcag cagccccaaa ttggtttttg
     1861 ccaaagtttc cgccattaaa tcgaaactga cgcggtttcc ttcgatttcg gtaatcgtca
     1921 ggcagcagcc gttattggcg accgatgcgc cgcgttgcag attgtccgcc gcctcttgcg
     1981 gaagctcgac gacataagtg tgaaatgcct ccgacgggcg gtggattgcc gtcagttttc
     2041 ccaatccttg aacaatgcct gtaaacataa tcctgtttcc ctgtgtcggt aaaaatggtg
     2101 caaattgtag catctccccg cgaaaaatgc cgtctgaaat gccttcagac agcattatgc
     2161 ctccgattcg ggcaaaaacc gcccggtatg gcttgacctt tcctttccac gccggtcggc
     2221 ggtcttgccc ttatccctcc tgcaaatcga tttgcgtgtt caagtcggca aaatgcccgt
     2281 caaactcgaa tctgaccggc cgcgcccttt gctgctgcaa ccagcttctt aatgttttca
     2341 tacccgaaaa cagataggga atcgcgcttt gcagaatctg cgggcggata tacataatgt
     2401 tgtagtgcat cgttatcggc gtttccacat aatacgcatt gcacaacggt gtgcgtttcg
     2461 acacggtttc aaaccttgcc accaaatcgt ccggcagata cggcatatcg cacggcacaa
     2521 ccaaaagcca gtcagcagtc gccaactgca aatcgttggc tgcggtacac aatgccgaaa
     2581 gcgggccgaa atgctgccac tgccgtgcgt ccggaaaaat atgcggactt cttcgagcat
     2641 attcttccaa attccggttg gtgctgatgg cgatatggct gacctgcggc ctgaccctgt
     2701 cgatgacatg atctatcagt gccttacccc ccaaaagagc aagccctttg tcctcgcctc
     2761 ccatacggct cgcctgaccg ccggccagta tcagggcaaa agttttcatt gcggatgttc
     2821 tcttggaaaa gttcgaggtt ttcatgattg cagtctgccg ttcccaatga ctcaaaatgc
     2881 cgtctgaagc agacggcaaa taaattcata ttatctgaat tttatcataa catgatttaa
     2941 cactgaagcg gtgcggatta agttttcctt aaccaattct ttctgagcag ttgtatctaa
     3001 tcccaaagaa tgatattgtt tgcattattt agaacaattt ttcgccgagc atgatactgc
     3061 cagcccgttt ttcagacggc atcagccttt ccctacgcct gaaactcctg accggactgt
     3121 gggtcgggtt ggcggcattg tctgtcgttt tgacactgct gctctctttg cgtctggaaa
     3181 acgcggcctc cgtcatcgaa gaggcgggca acttgagaat gcaggcatac cgtctggcat
     3241 acatggcggg tgaaggctcg ccccgtgcgc aaattgacaa tcaggttgcc gaatttgaaa
     3301 aaagcctgaa tcgtatttca caaagtgatg ccatccatcc gctgattcct tcggacaccc
     3361 ctcttgctta tgatttgata caatccatgc tgattataga ttggcaggca cacatcctcc
     3421 ccccgctcca gtcctaccgg cgaccgactc aggtcgatct ctaccgcttt gccggaaaca
     3481 tcgaactgtt tttgcaggca ttggaaaatg ccaacgaaaa aaacacatgg tggctcaggc
     3541 gttttcaatg ggcaattatg ttgatgacgc tggtgtcgtc tgtactgatg ctgttttggc
     3601 accagatttg ggttatccgg ccgctgcagg cgttaaggga aggtgcggaa cgcatcggac
     3661 ggaggtgttt cgatattccg gttcccgaag gcggtacgcc ggaattcaaa caggtcgggc
     3721 gttgtttcaa tcaaatgggc ggcaggttga aaattttata tgatgatttg gaaggacaag
     3781 tcgccgagca gacacgcagt ctcgaaaaac aaaatcaaaa cctgaccctg ctgtaccaaa
     3841 ctacacggga cctgcaccaa tcctacatac cgcaacaggc tgcagaacat tttctaaacc
     3901 gtatcctgcc cgccgtagga gcagattccg gcagagtttg tttggacggc ggatccgatg
     3961 tttatgtttc cattcatcat gcggattgcg gcacagcagc ttcggatttg gggaagtacc
     4021 atgaggaaat cttccccatt gagtaccaga acgaaacatt gggcaggctg ttgctcagct
     4081 ttccaaacgg catttctctt gatgaagacg accgcatcct gcttcaaaca ctaggcaggc
     4141 aattgggcgt atcgcttgcc ggcgcaaaac aggaggaaga aaaacgcctg cttgcagtat
     4201 tgcaggaacg caacctgatt gcgcaaggat tacatgacag catcgcacaa gcattaacgt
     4261 tcctaaacct acaggtacag atgctggaaa ccgcctttgc cgaaaacaaa cgggaggaag
     4321 ccgcagaaaa catcggcttc atcaaaacag gcgtgcagga atgttatgaa gatgtccgcg
     4381 aactgctgct caacttccgt accaaaatca gcaataaaga atttcccgaa gccgttgccg
     4441 acctattcgc ccgctttacg caacaaaccg ggataacggt cgaaaccgcc tgggaaaacg
     4501 gttcgttcct gccgcctcag gaagcgcagc tccaaatgat ttttatcctg caggaaagcc
     4561 tgtccaacat ccgcaaacac gcccgcgcca cccatgtaaa attcaccctt tccgaacacg
     4621 gcggacgctt taccatgacc atccaagaca acggacaagg tttcgacacg gagaaaatag
     4681 gagaacccac gggcagccat gtcggactgc acatcatgca ggagcgtgcc aaacgcatcc
     4741 atgccgtttt agaaatccgt tcccaagctc aacagggaac caccgtctca ttgacggttg
     4801 catctgaaga aagcttgaaa tgaccattaa aattatcctg atagacgacc ataccctctt
     4861 ccgcagcggc attaaagccc ttttgtcgcg ccaacacggt tttgaagtca tcggcgaagc
     4921 cgcagacggc ctctcgggca tcaaaatgat tagtcggctg caacccgatg tcgtcctgct
     4981 cgatttggat atgcccggca tgaacggacg cgaagcactc tcccaaatca tcagcatcaa
     5041 tccgcagcag gcagtgatta tgctgaccgt ttccgaagac agcgacgact tgaccgaatg
     5101 tattcgcatc ggcgcgcgcg gttacctgct gaaaaacatc aacgctgact ttctgctcga
     5161 aagcatacgc aaagccgccg aaggcgataa tgtattctcg cccgagatga ccgccaaact
     5221 cgttaaaagc ctgatttccc cccaacctgc ccaagggaca caggcactct cctcacttac
     5281 ccctcgcgaa ctggaaatct tgggctatct cgccgcagga cacagcaaca aaatcatcgc
     5341 ccgccacctc gatcttgccg aatccaccgt caaagtccac gttcaaaacc tgctccgcaa
     5401 actcaacctc agcagccggg tgcaggccgc tgtttacgcc atccggcaca acgtccccca
     5461 acccgtgctg gaataaggcg ggttcagacg gcatattagg ggttttaatc cccgtacggt
     5521 cattcggata acagaccaag catgtaagtt tatgccccca taagtacgct tggcatagca
     5581 gtaatattgt tcggtttagt gttttccgtt tgcccctatc tgatactgca atatcagcta
     5641 tgccgtctga aaacgtatca tcatgatatt ttcagacggc ataataaaaa gcggaa
//
LOCUS       DAXHSF010000131         5605 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.131, whole genome shotgun sequence.
ACCESSION   DAXHSF010000131 DAXHSF010000000
VERSION     DAXHSF010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5605
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.131"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(<1..297)
                     /locus_tag="ACFM8S_001639"
     CDS             complement(<1..297)
                     /locus_tag="ACFM8S_001639"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFC6314689.1"
                     /translation="MNILLLDGGKAFGHSHGGLNRTLHKKAKEVLTALGHNVQETVID
                     AGYDVEAEIEKFVWMDAVIWQMPGWWMHEPWTVKKYIDEVLTAGHGKLYQSDGRH"
     gene            536..1435
                     /gene="crgA"
                     /locus_tag="ACFM8S_001640"
     CDS             536..1435
                     /gene="crgA"
                     /locus_tag="ACFM8S_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151940473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator CrgA"
                     /protein_id="HFC6314690.1"
                     /translation="MKTNSEELTVFVQVVESGSFSRAAEQLAMANSAVSRIVKRLEEK
                     LGVNLLNRTTRQLSLTEEGAQYFRRAQRILQEMAAAETEMLAVHEIPQGVLRVDSAIP
                     MVLHLLAPLAAKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLF
                     DSCFRVIASPEYLAKHGTPQSTEELAGHQCLGFTEPGSLNTWAVLDAQGNPYKISPHF
                     TASSGEILRSLCLSGCGIACLSDFLVDNDIAEGKLIPLLAEQTSDKTHPFNAVYYSDK
                     AVNLRLRVFLDFLVEELGNNLCG"
     gene            complement(1662..4877)
                     /gene="carB"
                     /locus_tag="ACFM8S_001641"
     CDS             complement(1662..4877)
                     /gene="carB"
                     /locus_tag="ACFM8S_001641"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFC6314691.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPIMWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     ARNGVLAKYNVELIGATEDAIDKAEDRGRFKEAMEKIGLSCPKSFVCHTMNEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNKDEFLAICERGFDASPTHELLIEQSVLGWKEYEM
                     EVVRDKNDNCIIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VNTGGSNVQFAVNPENGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFTLDE
                     LRNDITGGKTPASFEPSIDYVVTKIPRFAFEKFPAADDRLTTQMKSVGEVMAMGRTIQ
                     ESFQKALRGLETGLCGFNPRSSDKAEIRRELANPGPERMLFVADAFRAGFTLEEIHEI
                     CAIDPWFLAQIEDLMKEEKAVSDGILSDLDFAALRRLKRKGFSDKRLAQLLNVSEKEV
                     REHRYALKLHPVYKRVDTCAAEFATETAYLYSTYEEECESRPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHAALALRESGFETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RTENPWGVIVHYGGQTPLKLANALVENGVNIIGTSADSIDAAEDRERFQKVLNDLGLR
                     QPPNRIAHNEEEALVKAEEIGYPLVVRPSYVLGGRAMQIVHSAEALQKYMREAVQVSE
                     DSPVLLDFFLNNAIEVDVDCVSDGKDVVIGGIMQHVEQAGIHSGDSGCSLPPYSLSEE
                     IQDEIRRQTKAMAYALGVVGLMNVQFAVQDGVVFVLEVNPRASRTVPFVSKATGVPLA
                     KVGARCMAGISLKEQGVEKEVVPDFYAVKEAVFPFIKFPGVDTILGPEMRSTGEVMGV
                     GASFGEAYYKAQLGAGERLSPTGKIFLSVREEDKERVIKTAKNFQALGYGICATRGTA
                     QYLTEHGLIVQTINKVPEGRPHIGDALKNGEIALVVNTVSSDPQSVSDSHIIRQSALQ
                     QRVPQYTTTAGGEAMSEGAKSRDHLGVYSVQELHGRLKNRN"
     gene            complement(4884..5432)
                     /locus_tag="ACFM8S_001642"
     CDS             complement(4884..5432)
                     /locus_tag="ACFM8S_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314692.1"
                     /translation="MRTIFFSGSRSISRLNPQIRERINNILSNNFDIVIGDANGADKA
                     IQKFLQEQDYANVHIYFSGKIYRNNVGNWQFVQVDSKGTGRVFYTAKDKKMAEIADYG
                     FILWDGKSIGSLNNIAELLQLNKPSLVYHSQTKEFFKIKSSADLENILSNIEDDVLAS
                     ILEKGNTFLKSYVTKQPSLIQE"
BASE COUNT         1277 a         1417 c         1429 g         1482 t
ORIGIN      
        1 gtgtctgccg tcgctttggt agagtttgcc gtgtccagcg gttaatactt cgtctatgta
       61 ttttttcact gtccaaggct cgtgcatcca ccagcccggc atctgccaaa tcacagcatc
      121 catccaaacg aatttttcga tttctgcctc aacatcatag ccggcatcaa tcacggtttc
      181 ttgaacattg tgtccgagcg cggtcaaaac ttctttcgct tttttgtgaa gcgtgcggtt
      241 taacccgccg tgagaatgtc cgaacgcttt gccgccgtct aataataaaa tattcatttg
      301 ttacctcgtt tgtgaattga tgaatgtatt gtgccgtttt actttataat ttaaaagtgc
      361 aaaaataaga aaacactttt gcgtcaaatg aaataatcag atgttccaac acacgggatg
      421 gcacataaag caccgccctg tgtgccgtcc tgattcggaa ggggttacac ccctcccaaa
      481 taaagtctga tcctgccgcc ctaaagggcg gggttccaac cgaaaaggaa acacgatgaa
      541 aaccaattca gaagaactga ccgtatttgt tcaagtggtg gaaagcggca gcttcagccg
      601 tgcggcggag cagttggcga tggcaaattc tgccgtaagc cgcatcgtca aacggctgga
      661 ggaaaagttg ggtgtgaacc tgctcaaccg caccacgcgg caactcagtc tgacggaaga
      721 aggcgcgcaa tatttccgcc gcgcgcagag aatcctgcaa gaaatggcag cggcggaaac
      781 cgaaatgctg gcagtgcacg aaataccgca aggcgtgttg cgcgtggatt ccgcgatacc
      841 gatggtgctg catctgctgg cgccgctggc agcaaaattc aacgaacgct atccgcatat
      901 ccgactttcg ctcgtttctt ccgaaggcta tatcaatctg attgaacgca aagtcgatat
      961 tgccttacgg gccggagaat tggacgattc cgggctgcgt gcacgccatc tgtttgacag
     1021 ctgcttccgc gtaatcgcca gtcctgaata cctggcaaaa cacggcacgc cgcaatctac
     1081 agaagagctt gccggccacc aatgtttagg cttcaccgaa cccggttctc taaatacatg
     1141 ggcggtttta gatgcgcagg gaaatcccta taagatttca ccgcacttta ccgccagcag
     1201 cggtgaaatc ttacgctcgt tgtgcctttc aggttgcggt attgcttgct tatcagattt
     1261 tttggttgac aacgacatcg ctgaaggaaa gttaattccc ctgctcgccg aacaaacctc
     1321 cgataaaaca caccccttta atgctgttta ttacagcgat aaagccgtca atctccgctt
     1381 acgcgtattt ttggattttt tagtggagga actgggaaac aatctctgtg gataattcta
     1441 taaaaacctg tcataaaact gtttttactc acttaataac tttgagaaaa aataaataag
     1501 atgatgtata gccggcggca atggcagtct atatggggag cgtgataggg ctgttcctta
     1561 tgtacggctt gtgtgtggca acatcggctt tccaaatgac aaaatgccgt ctgacaccgt
     1621 aaaaaaaggt ttcagacggc attttcaacc tgattcaggc atcagttgcg gtttttcaaa
     1681 cgcccgtgca actcttgaac gctgtacacg cccagatggt ctcggctttt cgcgccttcg
     1741 ctcatcgctt cgccgccggc ggtggtggtg tattgcggca cacgttgctg caatgcgctt
     1801 tggcggatga tgtggctgtc ggacacggat tgcggatcgc tggaaacggt gttcacgacc
     1861 agtgcgattt cgccgttttt cagcgcgtcg ccgatgtgcg ggcggccttc ggggactttg
     1921 ttgatggtct gcacaatcag cccgtgttcg gtcaggtatt gcgccgtgcc gcgcgtggcg
     1981 cagatgccgt agcctaaggc ttggaagttt ttagcggttt tgatgacgcg ttctttgtct
     2041 tcttcgcgca cggagaggaa gattttgccg gtcgggctga ggcgttcgcc cgcgccgagt
     2101 tgggctttgt agtaggcttc gccgaaactt gcgcccacgc ccatcacttc gccggtggag
     2161 cgcatttccg gtcccaaaat cgtatccacg cccgggaatt tgatgaatgg gaacacggct
     2221 tctttaacgg catagaaatc ggggacaact tctttttcca cgccttgttc tttcagggaa
     2281 atgcctgcca tacagcgtgc gccgactttg gcgagcggca cgccggtggc tttggagacg
     2341 aagggcacgg tacggctggc gcgcgggttc acttccaaca cgaacaccac gccgtcttgt
     2401 acggcaaact gcacgttcat cagtccgacc acgcccagcg cgtacgccat cgctttggtt
     2461 tggcggcgga tttcgtcttg gatttcttcg cttaaggagt agggcggcag cgagcagcca
     2521 gagtcgccgg agtgaatgcc tgcctgttcg acgtgctgca taatgccgcc gataaccacg
     2581 tctttgccgt ctgaaacgca gtccacatcc acttcaatcg cgttgttgag gaagaaatcg
     2641 agcaacacgg ggctgtcttc ggaaacctgc acggcttcgc gcatgtattt ttgcaaggct
     2701 tcggcagagt ggacaatctg catcgcgcgt ccgcccaaaa cataagacgg gcgcacgacc
     2761 agcggatagc cgatttcttc ggctttgacg agtgcttctt cttcgttgtg ggcgatgcgg
     2821 ttgggcggtt ggcgcagacc taagtcgttc aacacttttt ggaagcgttc gcggtcttcg
     2881 gcggcgtcga tgctgtcggc ggacgtgccg atgatgttta cgccgttttc aaccaacgcg
     2941 ttggcgagtt tcagcggggt ttgaccgccg taatgcacaa tcacgcccca cgggttttcg
     3001 gtgcggacga tttccaacac gtcttccaat gtcagcggct cgaaatagag gcggtcgctg
     3061 gtgtcgaagt cggtggacac ggtttctggg ttgcagttga ccatgatggt ttcaaagccc
     3121 gattcgcgca gggcgagtgc ggcgtgaacg cagcagtagt caaactcgat gccctgaccg
     3181 atgcggttcg ggccgccgcc gagaatcatc acttttttac ggtcggaagg acgggattcg
     3241 cattcctctt cgtaagtgga gtagaggtag gcggtttcgg tggcaaactc ggcggcacag
     3301 gtgtcgacgc gtttgtaaac cggatgcagc ttcagcgcgt agcggtgttc gcgaacttct
     3361 ttttcgctta cgttcaacaa ttgtgccaaa cgtttgtcgg agaagccttt gcgtttcaga
     3421 cggcgtaggg cggcgaaatc caaatcactc aaaatgccgt ctgaaaccgc tttttcttcc
     3481 ttcatcaagt cttcgatttg cgccaggaac caagggtcga tggcgcagat ttcgtggatt
     3541 tcttccagcg tgaagcccgc gcggaacgcg tctgccacaa acagcatacg ttcggggccg
     3601 gggtttgcca gttcgcggcg gatttccgct ttgtcggagc ttctcggatt gaagccgcac
     3661 aagcctgttt ccaagccgcg cagggctttt tggaagcttt cctgaatcgt gcggcccatc
     3721 gccatcactt cgcccacgga tttcatctgc gtggtcaggc ggtcgtctgc ggcagggaat
     3781 ttttcaaatg cgaaacgcgg gattttggtt accacatagt cgatggaagg ctcgaacgac
     3841 gcgggggttt tgccgccggt gatgtcgttg cgcaactcgt ccagcgtaaa gccgaccgcc
     3901 agttttgccg ccaccttcgc aatcgggaag cccgtcgctt tggaagccag cgcggacgaa
     3961 cggctcacgc gcgggttcat ctcaatcaca atcatctcgc cgttttcagg gttcaccgca
     4021 aactgcacgt tcgagccgcc cgtgttcacg ccgatttcgc gcaataccgc caacgaagcg
     4081 ttgcgcatga tttggtattc tttgtccgtc aacgtttgcg ccggcgcaac cgtaatcgag
     4141 tcgcccgtat gcacgcccat cgggtcgaag ttttcaatcg agcagatgat gatgcagttg
     4201 tcgtttttat cgcgtacgac ttccatctcg tactctttcc agccaaggac ggactgctca
     4261 atcagcagct cgtgcgtggg cgaggcatcg aaaccgcgtt cgcaaatcgc caaaaactcg
     4321 tctttattgt aggcaatgcc gccgcccgaa ccgcccatgg tgaaagaagg acgaatcagg
     4381 gtagggaagc cgacctgttc ttgcgccgcc aaagcttcgt tcatggtgtg gcagacaaaa
     4441 gatttcgggc aagataaacc gattttttcc atcgcctcct taaagcgacc gcggtcttct
     4501 gctttgtcga tggcgtcttc cgtcgcaccg atcaactcga cattgtattt cgccagcacg
     4561 ccgttgcgcg ccaaatccag cgcacagttc agcgcggtct gaccacccat cgtgggcaga
     4621 atcgcatcgg gccgctcctt ggcgataatc ttctccaccg tctgccacat aatcggctcg
     4681 atgtaggtaa catccgccat ttcagggtcg gtcatgatgg tggcggggtt agaattcacc
     4741 agaatgactt tatagccttc ttcacgcaaa gccttgcagg cctgtgcgcc cgaatagtca
     4801 aattcgcagg cctgaccgat aacgataggg ccggcgccga tgataaggat ggattttagg
     4861 tcggtacgtt tgggcatggg tggttactct tgaattaaag aaggttgttt tgttacatag
     4921 cttttaagaa aagtatttcc cttctctaaa attgaagcta aaacatcatc ttctatgtta
     4981 gataaaatat tctctaaatc tgcgcttgat ttaattttaa agaattcttt tgtttgagaa
     5041 tggtatacaa gagaaggttt atttagctgt aaaagttctg ctatattatt taatgaacca
     5101 atactcttac catcccataa aataaagcca taatcagcaa tttctgccat tttcttatct
     5161 tttgctgtat aaaataccct tccagttcct ttgctgtcta cttggacaaa ctgccaattc
     5221 cccacattat ttcggtaaat tttgccagaa aagtaaatat gcacattagc ataatcttgt
     5281 tcttgtagaa atttttggat agctttatca gccccattag catctccaat aacaatatca
     5341 aaattattgc taagaatatt gttaattctt tctcgtattt gtggatttag tcgtgaaatg
     5401 ctgcgtgaac cagaaaagaa aatagtgcgc atttttatcc tctagtcttt gtcattgcta
     5461 aaacaattac agatctagca ttttttgaaa acaatttttc agttgatatt tccaatgttg
     5521 ctccgctatc aaataaatca tcaattacta aaatattctt attggataga tctatattgt
     5581 caattgtgat ggaattattc agtat
//
LOCUS       DAXHSF010000132         5501 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.132, whole genome shotgun sequence.
ACCESSION   DAXHSF010000132 DAXHSF010000000
VERSION     DAXHSF010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5501
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.132"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(1..548)
                     /locus_tag="ACFM8S_001643"
                     /pseudo
     CDS             complement(1..548)
                     /locus_tag="ACFM8S_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor"
     gene            complement(619..1029)
                     /locus_tag="ACFM8S_001644"
     CDS             complement(619..1029)
                     /locus_tag="ACFM8S_001644"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314693.1"
                     /translation="MGNTAMWMSCNPTIPILFGIQVKIFPLNQILNHIHPARYAVTFE
                     NNVDSKLRRHWVAGATIRIIDRQTDEVIAKKTIYVFEKGLDGTGGARMPWKFAILCNK
                     ERLTSSEPLSDFVLSVLKPYILRPLYIASLRRDD"
     gene            complement(1153..1506)
                     /locus_tag="ACFM8S_001645"
     CDS             complement(1153..1506)
                     /locus_tag="ACFM8S_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314694.1"
                     /translation="MFTTNDLRHFLEGLAILFSIGYWGTMLLLLWFLVRFAYKKPKRN
                     PGKILSGSLTAAAILMLFVWIIPKQFGPIKEEIQAQEEWDRKYKEAEAVFNEQCKTAG
                     ERFTRRRTMWKGLCC"
     gene            1742..3169
                     /locus_tag="ACFM8S_001646"
     CDS             1742..3169
                     /locus_tag="ACFM8S_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFC6314695.1"
                     /translation="MFFSALKSFLSRYITVWRNVWAVRDQLKPPKRTAEEQAFLPAHL
                     ELTDTPVSAAPKWAARFIMAFALLALLWSWFGKIDIVAAASGKTVSGGRSKTIQPLET
                     AVVKAVHVRDGQHVKQGETLAELEAVGTDSDVVQSEQALQAAQLSKLRYEAVLAALES
                     RTVPHIDMAQARSLGLSDADVQSVQVLAQHQYQAWAAQDAQLQSALRGHQAELQSAKA
                     QEQKLVSVGAIEQQKTADYRRLRADNFISEHAFLEQQSKSVSNWNDLESTRGQMRQIQ
                     AAIAQAEQNRVLNTQNLKRDTLDALRQANEQIDQYRGQTDKAKQRQQLMTIQSPADGT
                     VQELATYTVGGVVQAAQKMMVVAPDDDKMDVEVLVLNKDIGFVEQGQDAMVKIESFPY
                     TRYGYLTGKVKSVSHDAVSHEQLGLVYTAVVSLDKHTLNIDGKAVNLTAGMNVSAEIK
                     TGKRRVLDYLLSPLQTKLDESFRER"
     gene            3238..4641
                     /locus_tag="ACFM8S_001647"
     CDS             3238..4641
                     /locus_tag="ACFM8S_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFC6314696.1"
                     /translation="MTLLNLMIMQDYGISVCLTLTPYLQHELFSAMKSYFSKYILPVS
                     LFTLPLSLSPSVSAFTLPEAWRAAQQHSADFQASHYQRDAVRARQQQAKAAFLPHVSA
                     NASYQRQPPSISSTRETQGWSVQVGQTLFDAAKFAQYRQSRFDTQAAEQRFDAAREEL
                     LLKVAESYFNVLLSRDTVAAHAAEKEAYAQQVRQAQALFNKGAATALDIHEAKAGYDN
                     ALAQEIAVLAEKQTYENQLNDYTDLDSKQIEAIDTANLLARYLPKLERYSLDEWQRIA
                     LSNNHEYRMQQLALQSSGQALRAAQNSRYPTVSAHVGYQNNLYTSSAQNNDYHYRGKG
                     MSVGVQLNLPLYTGGELSGKIHEAEAQYGAAEAQLTATERHIKLAVRQAYTESGAARY
                     QIMAQERVLESSRLKLKSTETGQQYGIRNRLEVIRARQEVAQAEQKLAQARYKFMLAY
                     LRLVKESGLGLETVFAE"
     gene            complement(4777..5501)
                     /locus_tag="ACFM8S_001648"
                     /pseudo
     CDS             complement(4777..5501)
                     /locus_tag="ACFM8S_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153708080.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         1398 a         1348 c         1412 g         1343 t
ORIGIN      
        1 gaaaagtcgt gattcccagc cttcttttgc gacgccgcaa ccggtgccgg tcagttcgaa
       61 aaggatggta ttgttttgtc cgccaaaatg gacgcgaccg tataggacgt cttccgaacc
      121 catcaaccaa cagcgctcat aaaaacggcc gcccgagcct ttggactctt tgtagatacc
      181 gaaaccgaaa acttcttcgg cgagcatgga cgcggcgcga ataaaatctt cgtcttccaa
      241 aagacttaca cgaacgccgt atttatcgaa aaagggtttt tcatgaaatg aaaagctaat
      301 ttgatcaatg aaagccgaat ctgatacacc gcgccgaagc ggaacgccta acaggtttcc
      361 tttgccgtcc gttatgtacg tttcgtaaca ttcgaagact tcctgaaccc tgcctgccgt
      421 ttcggtttct gtccccccct gttagataag gggggaagat ttgaagcggt tgccggcttc
      481 ctgccgtccg ccggtgcgtc cgtcatcacg ccgacaaccg cctttgtcat cccttgctta
      541 tcttccatgg tgagaatcct caaaaacggg caaaaaaaag ccctgttact tgtagaaagt
      601 aaaggacgtt aatttttgtt aatcgtccct tcttagggac gcaatatata agggacgcaa
      661 tatataaggt tttaaaacgc taagaacaaa atccgataac ggctctgaag aagtaagtct
      721 ttctttattg cacaagatag caaacttcca cggcattctc gccccacccg tgccgtccaa
      781 gcctttttca aagacataga tggttttctt ggcaatcact tcgtcagttt ggcggtcgat
      841 aatccgtatg gtcgcacctg ccacccagtg cctgcgcagc ttggaatcga cattgttttc
      901 gaacgttacc gcataacggg cggggtgtat atgattaagt atttgattta gcggaaaaat
      961 ctttacctga ataccgaata atatcggaat ggttgggttg caggacatcc acatagcggt
     1021 attccccatc gctaaaaaat cctaggaaac gagcaataaa atttacgcct tcgctggtct
     1081 gtaaagccgc attgtcccac atcgggtctc tggtttttgc atctgccgaa acggtacgct
     1141 caggtactac cttcaacagc ataatccctt ccacattgtc cgccgtctgg taaatctttc
     1201 ccccgccgtt ttgcattgtt cgttaaacac ggcttcggct tctttgtatt ttctgtccca
     1261 ctcttcttgt gcctgtattt cttctttgat cgggccgaat tgtttgggaa taatccaaac
     1321 aaacagcatc aggatagcgg cggcggtcag gctgcctgaa aggattttgc cggggttccg
     1381 tttgggcttt ttataggcaa agcggacgag aaaccaaagc aacagcagca tggtgcccca
     1441 atagccgatt gagaatagga tggccaaacc ttctaggaaa tggcgtaaat cgtttgtggt
     1501 aaacatgggt tgttcctgtg gttaaatgtg caggctgctt tttgccgaac cttgccgcat
     1561 ctcaaaagca gcctgcgctt cagcgttgcg ttacgcagta aaataatgaa tatttgtaac
     1621 gacttgggta ttttttgtca atattcccgc ctttccctta acagctgccg cgctttccgt
     1681 taaaattcct ttacatattt atattgtttc ctgtttctat attgccaagg ttatacccgt
     1741 tatgtttttc tccgccctga aatcctttct ttctcgatac attactgtat ggcgcaatgt
     1801 ttgggcggtg cgcgaccagt tgaaaccgcc caaacgcacg gcggaagaac aggcgttttt
     1861 gcccgcgcat ttggaactga ccgatacgcc ggtctctgcc gctccgaaat gggcggcgcg
     1921 ttttattatg gcgtttgcgc ttttggcttt gttgtggtcc tggttcggca aaatcgatat
     1981 tgtggcggcg gcttcgggca aaacggtgtc gggcgggcgc agcaaaacca tccagccgct
     2041 ggaaacggcg gtggttaagg cggtacatgt gcgcgacggg cagcatgtga aacagggaga
     2101 aacgctggcg gaactggagg ctgtgggaac agacagcgat gtggtgcagt cggagcaggc
     2161 tttgcaggct gcccaattgt ccaaactgcg ttatgaagcg gtattggcgg cattggaaag
     2221 ccgtaccgtg ccgcatatcg atatggcgca agcacggtct ttaggtctct ccgatgccga
     2281 tgtgcaatcg gtgcaggtgt tggcgcagca ccagtatcag gcatgggcgg cgcaggatgc
     2341 gcaattgcag tcggctttgc gcggccatca ggcggaattg cagtcggcca aggcgcagga
     2401 gcagaagctg gtttcggtgg gggcgatcga gcagcagaaa acagcagact accgccgttt
     2461 gcgggccgac aattttattt cggaacatgc gtttttggag cagcagagca aatcggtcag
     2521 caattggaac gatttggaaa gtacgcgcgg tcagatgagg cagattcagg cggccattgc
     2581 acaggcggag cagaatcggg tgctgaatac gcagaacctg aaacgcgata cgctggatgc
     2641 gctgcgccag gcaaacgaac agattgacca ataccgcggc caaacggata aggcaaagca
     2701 gcggcagcag ctgatgacaa tacagtcgcc tgcggacggc acggtgcagg aattggccac
     2761 ctatacggtg ggcggtgtgg tgcaggctgc ccaaaaaatg atggtggttg cgcccgatga
     2821 cgacaaaatg gacgtggaag ttttggtatt gaacaaagac atcggttttg tggaacaggg
     2881 acaggatgcg atggtgaaga ttgagagctt tccctatacg cgctacggtt atctgacggg
     2941 caaggtgaaa agtgtcagcc atgatgcggt aagccacgaa cagttgggct tggtttatac
     3001 ggcggtggtg tcgctggaca aacatacctt gaatattgac ggcaaagcag tgaatctgac
     3061 ggcgggcatg aatgtctcgg cggagattaa aacgggtaaa cggcgggtgc tggattatct
     3121 gttaagcccg ctgcaaacca aattggacga aagctttagg gagcgatagg cggatccgta
     3181 ctgggcattt gttatccgcc ggttcggaca tgcagactgc ctgaaaccat tgcccggatg
     3241 acattgctca atctaatgat aatgcaagat tacggtattt ccgtttgcct gacactgacg
     3301 ccctatttgc aacatgaact attttcggct atgaaatcct atttttccaa atatatccta
     3361 cccgtttcac tttttacctt gccactatcc ctttccccat ccgtttcggc ttttacgctg
     3421 cctgaagcat ggcgggcggc gcagcaacat tcggctgatt ttcaagcgtc ccattaccag
     3481 cgtgatgcag tgcgcgcacg gcaacaacaa gccaaggccg cattccttcc ccatgtatcc
     3541 gccaatgcca gctaccagcg ccagccgcca tcgatttctt ccacccgcga aacacaggga
     3601 tggagcgtgc aggtgggaca aaccttattt gacgctgcca aatttgcaca ataccgccaa
     3661 agcaggttcg atacgcaggc tgcagaacag cgtttcgatg cggcacgcga agaattgctg
     3721 ttgaaagttg ccgaaagtta tttcaacgtt ttactcagcc gagacaccgt tgccgcccat
     3781 gcggcggaaa aagaggctta tgcccagcag gtaaggcagg cgcaggcttt attcaataaa
     3841 ggtgctgcca ccgcgctgga tattcacgaa gccaaagccg gttacgacaa tgccctggcc
     3901 caagaaatcg ccgtattggc tgagaaacaa acctatgaaa accagttgaa cgactacacc
     3961 gacctggata gcaaacaaat cgaggccata gataccgcca acctgttggc acgctatctg
     4021 cccaagctgg aacgttacag tctggatgaa tggcagcgca ttgccttatc caacaatcat
     4081 gaataccgga tgcagcagct tgccctgcaa agcagcggac aggcgcttcg ggcagcacag
     4141 aacagccgct atcccaccgt ttctgcccat gtcggctatc agaataacct ctacacttca
     4201 tctgcgcaga ataatgacta ccactatcgg ggcaaaggga tgagcgtcgg cgtacagttg
     4261 aatttgccgc tttataccgg cggagaattg tcgggcaaaa tccatgaagc cgaagcgcaa
     4321 tacggggccg ccgaagcaca gctgaccgca accgagcggc acatcaaact cgccgtacgc
     4381 caggcttata ccgaaagcgg tgcggcgcgt taccaaatca tggcgcaaga acgggttttg
     4441 gaaagcagcc gtttgaaact gaaatcgacc gaaaccggcc aacaatacgg catccgcaac
     4501 cggctggaag taatacgggc gcggcaggaa gtcgcccaag cagaacagaa actggctcaa
     4561 gcacggtata aattcatgct ggcttatttg cgcttggtga aagagagcgg gttagggttg
     4621 gaaacggtat ttgcggaata aagcaggctg aaacggttat gaaattccca aagcagcctg
     4681 caccccgttt cgaaagtgca ggctgctttg ggattgatcc gatactttca cattctcatt
     4741 gcctttaaat aaagcacagt taaaaacaca tttaaattaa gtgcgaatta attcaaatgt
     4801 atattcaatt aaaatcaaaa taggacagta gtgcatcgtc aaatcgggcg taatcagaca
     4861 aaacggttcg cagataccgc ttaatattcg cccacacttt ctcaatcggg ttgagctcag
     4921 gtgaataagg tgcaagaggc aataccttat gtcccaattt ttccgccatt tcccgtaaga
     4981 cacccatacg gtgaaatcgc gcattatcta aaataatcac cgatttttga gtcaatgcgg
     5041 gcagtaggca ttgctgaaac cacgcttcaa aaaagactct ggtcatcgta ttttgataaa
     5101 ccatcggagc aatcagccgg ttgccgactt gtgcggacac caaagataag cgttggtatc
     5161 tttttccact tatctgcgct ttcactattt gccctttcag gctgcgggca tagggacgga
     5221 acaggtagcg gtcaaatcct gtttcatcca aataaacacg ttggtagtcg gaaaattcgg
     5281 ccggctgtgt caaataatgc gttactttgg ccgggtcttg ttctttgtaa gtggtggtct
     5341 ttttttgcgc gttatcccca tctgtttgag tgcatagcaa atggtggctg ccgtacaatc
     5401 aaaatgtttg gcgatttcat gcagataggc atcctggtgt tgcccaacat attgagccgg
     5461 tttttgccta tccgatttga cggcatttag accggtaact t
//
LOCUS       DAXHSF010000133         5474 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.133, whole genome shotgun sequence.
ACCESSION   DAXHSF010000133 DAXHSF010000000
VERSION     DAXHSF010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5474
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.133"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(254..1513)
                     /gene="rho"
                     /locus_tag="ACFM8S_001649"
     CDS             complement(254..1513)
                     /gene="rho"
                     /locus_tag="ACFM8S_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFC6314697.1"
                     /translation="MHVSELQTLHISKLLELAEEHGIENANRFRKQDLVFAIVRQMMK
                     KGEGFTCSGTLEILPDGFGFLRSADTSYLAGPDDIYVSPTQIRRFNLHTGDTIEGSVR
                     VPKDNERYFALVRLDTINGDHPEVCRHKILFENLTPLFPTQQLKLERDLKSEENLTGR
                     AIDLISPIGKGQRALLVAPPKSGKTVMLQNIAHAVTANYPEVELIVLLIDERPEEVTE
                     MSRSVRGEVVSSTFDEPAQRHVQVAEMVLEKAKRMVEHKKDVVILLDSITRLARAYNT
                     VVPTSGKILTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVETGSRMDDVIYEE
                     FKGTGNMELHLDRRMAEKRLFPAININKSGTRREELLVPNDQLQRMWLLRKFLHPMDE
                     IEAAEFLIGKIKASKNNDDFFELMRGK"
     gene            complement(1733..4117)
                     /gene="ppsA"
                     /locus_tag="ACFM8S_001650"
     CDS             complement(1733..4117)
                     /gene="ppsA"
                     /locus_tag="ACFM8S_001650"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFC6314698.1"
                     /translation="MADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGG
                     FATTADAYRAFLAHNGLSERISAALAKLDVEDVAELARVGKEIRQWILDTPFPEQLDA
                     EIEAAWNKMVADAGGADISVAVRSSATAEDLPDASFAGQQETFLNINGLDNVKEAMHH
                     VFASLYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYDQVVF
                     VTSSYGLGENVVQGAVNPDEFYVFKPTLKAGKPAILRKTMGSKHIKMIFTDKAEAGKS
                     VTNVDVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQAR
                     PETVKSQEEGNRNLRRFAINGDKTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLKNGQE
                     VTVSCAEGDTGFIYAGLLDVQITDVALDNMPKAPVKVMMNVGNPELAFSFANLPSEGI
                     GLARMEFIINRQIGIHPKALLEFDKQDDELKAEITRRIAGYASPVDFYVDKIAEGVAT
                     LAASVYPRKTIVRMSDFKSNEYANLVGGNVYEPHEENPMLGFRGAARYVADNFKDCFA
                     LECKALKRVRDEMGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCEL
                     PSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRDSGLVSESFDERNPAVKVMLHLAIS
                     ACRKQNKYVGICGQGPSDHPDFAKWLVEEGIESVSLNPDTVIETWLYLANELNK"
     gene            4528..5349
                     /locus_tag="ACFM8S_001651"
     CDS             4528..5349
                     /locus_tag="ACFM8S_001651"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFC6314699.1"
                     /translation="MSSPRHVFYISDRTGLTAENIGEALLNQFGNLSFKRHTHPFVDT
                     PEKARAVVEKVNRSRQENGQRPIAFVSVVDDEIRRIIKGADAFQINFFETFLGLLEKE
                     LNTEATASEQGHHSIGNTKRYDARMEAVNFSLNHDDGVSDKNLQEADVILMGVSRSGK
                     TPTCLYLALQYGIRAANYPLIPDDLESADLPRMVKPYRDKLFGLTIQPERLQAIRQER
                     RPNSTYAKIDTCRSEVADAQSMFRRHGIPFANTTDKSVEELAVHILQACKLKRRF"
BASE COUNT         1175 a         1288 c         1592 g         1419 t
ORIGIN      
        1 aatttataca aaaatgagcg cggttcggcg caaccttgaa tcaagttccc gcatcggttt
       61 tcattgccgg tacggatgcg ttcaagccgg ctttgcaaag gccgcgcttt cggcaagcgg
      121 acacggacac tgccggcggt tgcgccgtta acggggggag ggaggagctg cgccggccgt
      181 gtgaatgaaa gtgccgtctg aaacccgatt ttcaggcttc agacggcatt tcgcattaat
      241 gcgggtggcg cgtttatttg ccgcgcatca gttcaaagaa atcatcattg tttttagagg
      301 ctttgatttt cccgattaaa aattcggctg cctcgatttc gtccatcggg tgcaggaact
      361 tgcgtaagag ccacatacgt tgcaactggt cgttcggcac aagcagctct tcgcggcgcg
      421 tgccggattt gttgatgttg atggcgggga agaggcgttt ttccgccata cggcggtcga
      481 ggtgcaattc catattgccg gtgcctttga attcttcgta aatcacatcg tccatacggc
      541 tgccggtttc aaccaatgct gtggcaatga tggtcagcga accgccttct tccacgttgc
      601 gcgccgcgcc gaagaaacgt ttgggacggt gcagtgcgtt ggcatcgaca ccgccggtca
      661 ggattttgcc cgaggtaggc acgacggtat tgtaggcgcg ggcaaggcgg gtaatcgaat
      721 ccagcaggat gaccacgtct tttttgtgtt ccaccatacg cttggctttt tcaagtacca
      781 tttcggcaac ttggacgtgg cgttgcgccg gctcgtcaaa tgttgaagaa accacttcac
      841 ctcggacgga gcggctcatt tcggtaactt cttcgggacg ctcgtcaatc aagaggacga
      901 tgagctcgac ttcaggatag tttgcggtaa cggcgtgggc aatattttgc aacatcacgg
      961 ttttaccgct tttgggcggg gcaaccaaga gggcgcgctg acctttgccg atgggggaaa
     1021 tcagatcgat ggcgcgtccg gtcaggtttt cttcggactt taagtcgcgt tccagcttca
     1081 actgttgggt cggaaacagc ggggtcaggt tttcaaacag gattttatgg cggcatactt
     1141 ccgggtggtc gccgttgatg gtatcaagcc tgaccagggc aaaatagcgt tcgttgtctt
     1201 ttgggacgcg cacgcttcct tcgatggtgt cgcccgtatg caggttgaag cggcggattt
     1261 gggtgggcga gacatagatg tcgtcggggc cggcaagata ggacgtgtcc gcgctgcgga
     1321 ggaagccgaa gccgtcgggc aggatttcaa gcgtgccgga gcaggtgaaa ccctcgcctt
     1381 ttttcatcat ctggcggacg atggcaaata cgaggtcttg tttgcggaat cggttggcgt
     1441 tttcgatgcc gtgttcttcc gccaattcta agagtttgga aatgtgcagg gtttgtaatt
     1501 cggagacgtg cataataatg atgtattttg aagaggaaaa agacaggcag atgccgtctg
     1561 aaagaagaag ctgaccgttg ccggttgctc ggggaagggg gaattgtagg cagtcggcgc
     1621 gtgggtgtca aatattatcg cggacggggc atcggcagga aatgccgtct gagcggagct
     1681 gcttggaaaa aaataccccc gcgcttttca ggctcggggg tatgggcatt gattatttgt
     1741 tcaattcatt cgccaaatac agccaagttt cgatgacggt atccgggttc agggaaacgc
     1801 tttcaatgcc ttcctcaacc agccatttgg cgaagtccgg atggtcggac gggccttgac
     1861 cgcagatgcc gacatatttg ttctgcttgc ggcaggcgga gatggcaagg tgcagcatca
     1921 ctttgacggc agggttgcgt tcgtcaaacg attcggatac caagccgctg tcgcggtcga
     1981 gaccgagggt cagttgggtc atgtcgttcg agccgatgga gaagccgtcg aagtattgca
     2041 ggaattgttc cgccaatacc gcgttgctcg gcagctcgca catcataatc aggcgcaggc
     2101 cgtttttgcc gcgttccaag ccgttttctt tcagggcttt gacaacggct tcggcttcgc
     2161 ccaaggtgcg gacgaacgga atcatgattt caacgttggt caaccccatt tcatcgcgga
     2221 cgcgtttcaa ggctttgcat tccaaggcga aacagtcttt gaagttgtcg gcgacataac
     2281 gcgccgcgcc ccggaagccc aacatcgggt tttcttcatg cggttcgtat acgttgccgc
     2341 cgaccaggtt ggcgtattcg ttggatttga agtcggacat acggacgatg gttttacgcg
     2401 gataaaccga tgcggccaaa gttgccacgc cttctgcgat tttatcgacg tagaagtcga
     2461 caggggacgc atagccggcg atgcggcggg taatttccgc tttcaattcg tcgtcttgtt
     2521 tgtcaaattc caacaaggct ttggggtgga taccgatttg gcggttgatg ataaattcca
     2581 tacgcgccaa gccgatgcct tcgctgggca ggttggcgaa gctgaatgcg agttcgggat
     2641 tgccgacgtt catcatgact ttaaccggtg ctttgggcat attgtctaag gcgacatcgg
     2701 taatctgtac gtccaacaga ccggcataga taaagccggt atcgccttcg gcacaggata
     2761 cggtaacttc ttgaccgttt ttcagcaatt cggttgcatt gccgcagccg acaacggcag
     2821 gaatgcccaa ttcacgcgcg atgatggcgg cgtggcaggt acggccgccg cggttggtca
     2881 cgatggcaga agcacgtttc atcacgggtt cccaatccgg atcggtcatg tcggtaacga
     2941 gtacgtcgcc ggcttcgacg gaatccatct cggaagcatc tttaatcagg cgcaccttgc
     3001 cctgaccgac tttctgaccg atggcgcggc cttcgcataa tacggttttg tcgccgttga
     3061 tggcgaagcg gcgcaggttg cggttgccct cttcttggga ttttacggtt tcgggacggg
     3121 cttgcaggat gtagagtttg ccgtccaagc cgtcgcgtcc ccattcgata tccatcgggc
     3181 ggccgtagtg tttttcgatg gtcagtgcgt aatgcgccaa ctcagtaatt tcttcgtcgg
     3241 taatggagaa gcggttgcgg tcttcctcgg ggacatcgac gttggttacg gatttaccgg
     3301 cttctgcttt gtcggtaaaa atcattttga tgtgttttga acccatggtt ttacgcagga
     3361 tggcgggctt gcccgctttg agcgtgggtt tgaacacata aaattcgtcc gggttgaccg
     3421 caccttgtac gacgttttcg cccagaccgt aagaggaggt aacaaagacg acttgatcgt
     3481 agccggattc ggtgtcgagg gtgaacatca cacctgatgc gccgctgtcg gaacgcacca
     3541 tgcgttgaac gccggcggaa agggcgacga tgtcgtgttc gaagcctttg tggacacggt
     3601 aagaaatggc acggtcgtta tacagggaag cgaatacatg gtgcatcgct tctttaacgt
     3661 tatccaagcc gttgatgttc aagaaggttt cctgttgtcc agcgaatgat gcgtccggca
     3721 ggtcttcggc agttgcggaa gaacgtacgg caacggaaat gtccgcaccg ccggcatcgg
     3781 caaccatttt gttccatgcc gcttcgattt cggcatcgag ctgttcgggg aaaggcgtat
     3841 ccaaaatcca ttggcggatt tctttgccga cgcgtgccag ttcggcaacg tcttcgacat
     3901 ccaattttgc cagtgcggcg gaaatgcgtt cgctcagacc gttgtgtgcg aggaatgcgc
     3961 ggtaggcatc ggctgtggtg gcaaagccgc cggggacgcg aacgcctttt tcggtcagct
     4021 gactgatcat ttcgcccagc gaggcgtttt taccgcccac gcgttcaaca tctgtcatac
     4081 gcaggttttc aaaccagatt acgtagttgt cggccatttg tgtgtccaat ccaaaatatg
     4141 ttaaaaaaga aacaaatccg cgtgcttatt ttaagcgatt cgttcctctg ctgtcatgtg
     4201 ttttatccat tttaaaatca tgatgccgtc tgaaaaattg cggtttcggc gtgtgtagcg
     4261 gtttgaaact tgcagccggt atacttcttt ttttgggtat tttctttgta aaacaggtgg
     4321 tttgaatagg ttaatgtttt ttctgtttga tttttttgtt tattttttaa aattttctgc
     4381 caaaaaatac tttatataaa taattttatt tcaaaattat attgtgtctg tttgggtgta
     4441 atccgaggta ggcgtgctgc gggtgctttc cttgtgtctg ccgctgctgt tatgatgata
     4501 tttttattct gtatttaagg atggaagatg agcagtccgc gccatgtgtt ttacatttcc
     4561 gaccgtaccg gtctgactgc cgagaatatc ggcgaggctt tgctgaacca gtttggcaat
     4621 ctgtcgttca aacgccatac gcatccgttt gtcgatacgc cggaaaaggc gcgcgcggtg
     4681 gtggagaagg tcaatcggag ccggcaggaa aacggtcagc gtccgattgc gtttgtcagt
     4741 gtggttgatg acgaaatccg tcggattatc aaaggggcgg atgcttttca gattaatttc
     4801 tttgagactt ttttgggact gttggagaaa gaactcaata ccgaagccac ggcatccgag
     4861 caggggcatc acagtatcgg taatacgaag cgttacgatg cgcgtatgga ggcggtgaat
     4921 ttctcattga accacgatga cggggtcagc gataagaacc ttcaggaagc ggatgtaatc
     4981 ttgatgggtg tatcgcgttc gggcaaaacg ccgacctgcc tttacctcgc cctgcaatac
     5041 ggcatccgtg cggcaaacta tccgctgatt cccgacgatt tggaatcggc cgatctgccg
     5101 cgtatggtca agccttatag ggataagctg ttcgggttga ccatccagcc ggaacgtttg
     5161 caggccatcc gccaagagcg ccgcccgaat tcaacttatg ccaaaatcga tacatgccgc
     5221 agcgaggtgg cggacgcgca gagtatgttc agacggcatg gcattccgtt tgcgaatacg
     5281 acggataaat cagtggaaga gcttgccgta cacatccttc aggcgtgcaa gctcaaacgc
     5341 aggttttgac gggctttgat tcggtttgaa ggcggaaatg ccgtctgaaa tcaggtttca
     5401 gacggcagtt ttatagtgga ttaacaaaaa tcaggacaag gcgacgaagc cgcagacagt
     5461 acaaatagta cgga
//
LOCUS       DAXHSF010000134         5414 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.134, whole genome shotgun sequence.
ACCESSION   DAXHSF010000134 DAXHSF010000000
VERSION     DAXHSF010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.134"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(112..249)
                     /locus_tag="ACFM8S_001652"
     CDS             complement(112..249)
                     /locus_tag="ACFM8S_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314700.1"
                     /translation="MPSERLQTAFFTQFPPFPIIPDNTVILKPVIPTQAGIQSVRFRF
                     F"
     gene            complement(267..3014)
                     /gene="tbpA"
                     /locus_tag="ACFM8S_001653"
     CDS             complement(267..3014)
                     /gene="tbpA"
                     /locus_tag="ACFM8S_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein TbpA"
                     /protein_id="HFC6314701.1"
                     /translation="MQQQHLFRLNILCLSLMTALPVYAENVQAGQAQEKQLDTIQVKA
                     KKQKTRRDNEVTGLGKLVKTADTLSKEQVLDIRDLTRYDPGIAVVEQGRGASSGYSIR
                     GMDKNRVSLTVDGLAQIQSYTAQAALGGTRTAGSSGAINEIEYENVKAVEISKGSNSV
                     EQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKNRGLTQSIALAGRIGGAEA
                     LLIHTGRRAGEIRAHEDAGRGVQSFNRLVPVEDDNQYAHFVVEEECKSGNYETCKSKP
                     KKDIVGKDERQTVSTRDYTGPNRFLADPLSYESRSWLFRPGFRFENKRHYIGGILEHT
                     QQTFDTRDMTVPAFLTKAVFDANKKQAGSLSGNGKYAGNHKYGGLFTNGENGALVGAE
                     YGTGVFYDETHTKSRYGLEYVYTNADKDTWADYARLSYDRQGIGLDNHFQQTHCSADG
                     SDKYCRPSADKPFSYYKSDRVIYGESHRLLQAAFKKSFDTAKIRHNLSVNLGFDRFGS
                     NLRHQDYYFQNAKRAYSLKTPPKNGNKNISPNGSTSTPYWVSIGGGNVVTGQICRLGN
                     NTYTDCTPRSINGKSYYAAVRDNVRLGRWADVGAGLRYDYRSTHSDDGSVSTGTHRTL
                     SWNAGIVLKPADWLDLTYRTSTGFRLPSFAEMYGWRAGVQSKAVKIDPEKSFNKEAGI
                     VFKGDFGNLEASWFNNAYRDLIVRGYEAQIENGKETAKGDPAYLNAQSARITGINVLG
                     KIDWNGVWDKLPEGWYSTFAYNRVRVRDIKKRADRTDIQSHLFDAIQPSRYVVGLGYD
                     QPEGKWGVNGMLTYSKAKEITELLGSRALLNGNSRNTKATARRTRPWYIVDVSGYYTI
                     KKHFTLRAGVYNLLNHRYVTWENVRQTAGGAVNQHKNVGVYNRYAAPGRNYTFSLEMK
                     F"
     gene            complement(3100..5259)
                     /locus_tag="ACFM8S_001654"
     CDS             complement(3100..5259)
                     /locus_tag="ACFM8S_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC6314702.1"
                     /translation="MNNPLVNQAAMVLPVFLLSACLGGGGSFDLDSVDTEAPRPAPKY
                     QDVSSEKPKAQKDQGGYGFAMRFKRRFWLPRGKEDEVKLKESDWEETGLPDDPKNLPK
                     RQKSVIDEVKTDDGSNNIHSSPYLMQSNHQNGSTNGGANQPKNEVTDYKDFKYVYSGW
                     FYKQAKSEIKRENGSSSAKSGDDGYIFYHGEKPSRQLPASEKVIYKGVWHFVTDTKRG
                     QKFNDILGTSKGQGDKYSGFSGDDDEQYSNKNEKTLQGGQEGYGFTSNLEVDFGNKKL
                     KGNLIRNNRVTNATTGEKHTTQYYSLEAQVTGNRFSGTATATDKPEKDGTKQHPFVSD
                     SSSLSGGFFGPQGEELGFRFLSNDQKVAVVGSAKTQDKAANGNTAAASGGTDAAASNG
                     AAGTSSENGKLTTVLDAVELTHGGTAIKNLDNFSNAAQLVVDGIMIPLLPEASESGKN
                     QADKGKKGKNGGTAFTRKFAHTPKSDEKDAQAGTPTNGAQTASNTAGDTNGKTKTYEV
                     EVCCSNLNYLKYGMLTRKNSKSAMQAGESSSQADAKTEQVEQSMFLQGERTDEKEIPK
                     EQNVVYRGSWYGHIANDTSWSGNASNATSGNRAEFTVNFDTKKINGTLTAENRQEATF
                     TIDGKIEGNGFSGTAKTADLGFDLDQSNTTGTPKAYITDAKVQGGFYGPKAEELGGWF
                     AYSDDKQTKNATDASGNGNSASSATVVFGAKRQQPVR"
BASE COUNT         1117 a         1376 c         1339 g         1582 t
ORIGIN      
        1 cttagcattg aatgtctaga ttcccacttt cgcgggaatg acgggatttg aggtttctgt
       61 ttttgatttt ccgccttcgc gggaatgacg gtttagaagt tacccgaaac ctcaaaaaaa
      121 ccgaaaccga acggactgga ttcccgcctg cgtgggaatg acgggtttca agattacggt
      181 attgtcgggg atgatgggaa acggtgggaa ttgtgtaaaa aatgccgtct gaagcctttc
      241 agacggcatt tgcggcgttt ggacgtttaa aacttcattt ccaagctaaa tgtgtagttg
      301 cgaccggggg cggcatatcg gttgtaaacg ccgacatttt tgtgttggtt gactgcgccg
      361 ccggcagttt gccgcacatt ttcccaagta acatagcggt ggttgaggag gttgtacacg
      421 cccgcacgga gggtaaagtg ttttttaatc gtataataac cggacacatc cacaatatac
      481 caagggcggg tacggcgcgc ggtggctttt gtattgcggc tgttgccgtt gagcaaagcc
      541 cggctgccca acaactctgt gatttccttg gctttggaat aagtcagcat accgttcaca
      601 ccccatttgc cttccggttg gtcatagccc aagccgacga catagcgcga aggttggatg
      661 gcatcaaaca gatgtgattg aatatcggtg cggtctgcgc gttttttgat gtcgcggaca
      721 cggacacgat tataggcaaa tgtagaatac caaccttcgg gcaatttatc ccatacgccg
      781 ttccaatcga ttttgcccaa aacattgatg ccggtaatcc gcgcgctttg ggcattgagg
      841 taagccgggt cgcctttggc tgtttctttg ccgttttcaa tttgcgcttc ataaccccgg
      901 acaatcaaat cgcggtaggc attgttgaac caacttgcct ccaagttgcc gaaatcgcct
      961 ttaaacacga tgccggcttc tttgttgaac gatttttccg gatcgatttt gaccgccttg
     1021 ctttgaacac ccgcccgcca gccgtacatt tccgcaaacg agggcaggcg gaagccggtt
     1081 gaagtgcggt aagtcaaatc cagccagtcg gcaggtttga ggacgatgcc ggcgttccag
     1141 gacagggtac ggtgcgtgcc ggtggaaacg ctgccgtcgt ccgaatgcgt gctgcggtag
     1201 tcgtagcgca accccgcgcc gacatccgcc cacctgccca aacggacatt gtcccgaact
     1261 gccgcgtaat agcttttgcc gttgatgctg cgcggcgtac agtccgtata agtattgttg
     1321 cccaagcggc agatttgccc cgtaaccaca tttcccccgc ctatgctgac ccaataggga
     1381 gtgcttgtgc tgccgttggg gctgatgttt ttattgccgt ttttaggggg cgttttcaac
     1441 gaataggcgc gtttggcatt ttgaaaataa taatcctgat ggcggagatt agagccaaag
     1501 cggtcaaacc cgagattcac gctcaggttg tggcggattt tggcggtatc gaaggatttt
     1561 ttgaatgccg cctgcaagag cctgtggctt tctccgtaaa tcacgcgatc ggatttgtaa
     1621 taggaaaacg gcttgtcggc actcgggcgg caatatttgt ccgaaccgtc ggcagaacag
     1681 tgtgtctgct gaaaatgatt gtccaaaccg atgccctgcc ggtcgtaaga gaggcgggca
     1741 taatccgccc aagtgtcttt atcggcattg gtatagacat attccaaacc gtagcggctt
     1801 ttggtgtgcg tctcgtcgta aaacacgccc gtaccgtatt ccgcgcccac cagcgcaccg
     1861 ttttcgccgt tggtaaacag tccgccgtat ttgtggttgc ccgcgtattt gccgttgccg
     1921 gacaaagaac ccgcctgttt tttatttgca tcaaaaaccg ccttggtcag gaatgccgga
     1981 accgtcatat cgcgcgtgtc gaaagtttgt tgcgtgtgtt cgagtatgcc gccgatgtag
     2041 tgccgtttgt tttcaaaacg aaaacccggg cggaacagcc acgaccggct ttcgtatgaa
     2101 agcggatcgg cgaggaagcg gttgggaccc gtgtagtctc gggtagaaac cgtttgccgt
     2161 tcgtctttgc cgacaatatc ttttttcggt ttgcttttac acgtttcgta attcccgctt
     2221 ttgcattctt cttcgaccac gaaatgagcg tattggttgt cgtcctcaac cggcaccagc
     2281 ctgttaaagc tctgaacgcc gcgtccggca tcttcgtggg cgcggatttc ccccgcgcgc
     2341 cgacccgtgt ggatcagcaa agcctccgca ccgccgatgc gccccgccag cgcgatggat
     2401 tgggtaagcc cccggttttt gccggaatag gcggttttac tctgaatgcc ccactgcctg
     2461 ccttccccga taacatcgtc ggcggttttg gtttgaaatg cgaccgaacc cgccaatgcg
     2521 ccgctgcctt gttcgaccga gtttgagcct ttgctgattt cgacagcttt gacgttttca
     2581 tactcgattt cattgattgc gccgctgctg cccgccgtcc tcgtcccgcc caatgccgcc
     2641 tgcgcggtgt aggactgtat ttgcgccaag ccgtccaccg tcaaggaaac gcggtttttg
     2701 tccataccgc gtatcgagta gcccgagctt gcgccgcgcc cctgttcgac cacggcgatg
     2761 ccggggtcgt aacgcgtcag gtcgcggata tcgagtacct gttccttgct gagggtgtcg
     2821 gcggttttga ccaatttgcc caaaccggtt acttcgttat cgcggcgggt tttctgtttt
     2881 ttggctttta cctgtatggt atccaactgt ttttcctgtg cttgtccggc ttgcacattt
     2941 tctgcataaa cgggcagcgc ggtcatcagc gacaggcata aaatatttaa tcggaacaaa
     3001 tgttgctgtt gcataatgtt tccctaatct tcgctttcag acggtatcgg aaggagcgat
     3061 gccgtcgaag ccttattctt gattgttcgg caaccgtgct tatcgcacag gctgttggcg
     3121 tttcgcaccg aataccacgg tcgcgctgct tgctgaattt ccattgccgg atgcatctgt
     3181 tgcatttttc gtttgtttat cgtccgaata ggcaaaccat ccgcccaact cttcggcttt
     3241 gggcccgtaa aaaccgccct gcaccttggc atctgtgata tatgccttag gcgtgccggt
     3301 ggtattgctt tgatcgagat caaaacctaa gtcagcagtt tttgccgtac cggaaaaacc
     3361 gttgccctca atcttaccat caatggtaaa ggttgcctcc tgcctgtttt cagcggttaa
     3421 cgtgccgtta atttttttcg tatcgaaatt cacagtaaat tccgccctgt tgccactcgt
     3481 tgcattggaa gcattgccgc tccagcttgt gtcgttggca atatgcccgt accaagaccc
     3541 ccgataaacg acgttttgct cttttggaat ctctttttca tcggtacgct cgccttggag
     3601 gaacatactt tgttcaactt gttccgtttt agcatcagct tgactactgc tttctcctgc
     3661 ctgcatcgcg gacttgctgt ttttgcgcgt caacattccg tatttcagat aattgaggtt
     3721 ggaacagcag acttcgactt cataggtttt tgttttgcca ttggtatcac ctgccgtatt
     3781 tgaagcggtt tgcgccccat tcgtcggcgt acctgcttgg gcgtcttttt catcgctttt
     3841 cggcgtgtgg gcaaatttgc gggtaaaggc tgttccgccg tttttacctt ttttaccttt
     3901 atctgcctga tttttcccgc tttcggaagc ctcgggcagg agcggaatca taatgccgtc
     3961 gacaaccagt tgggcggcgt tgctgaagtt gtcgagattt ttgattgctg tgccgccgtg
     4021 cgtcagctcg accgcatcca aaaccgtggt cagcttaccg ttttcagacg acgtgcctgc
     4081 cgcaccgttt gatgctgccg catctgtgcc gcctgaagcc gccgcagtat tgccatttgc
     4141 ggctttgtct tgggttttcg cgctgccgac aacggcaact ttttgatcgt tgctcaaaaa
     4201 gcggaaaccc aattcctcac cctgcgggcc gaaaaagccg ccgctcaaag aagacgagtc
     4261 ggaaacaaag ggatgttgtt tggttccgtc tttttcaggt ttgtcagttg ccgttgccgt
     4321 gccgctgaaa cggttgcctg ttacttgagc ctcaaggctg taatattggg tggtatgttt
     4381 ttcgccagta gtagcgtttg taactctatt attgcgtatt aaattaccct tcaatttttt
     4441 attgccgaaa tccacttcta aatttgaggt aaaaccataa ccctcttgac cgccttgtaa
     4501 agtcttttca tttttattag aatattgttc atcatcatcg cccgaaaatc cgctgtactt
     4561 atctccttgt ccttttgagg ttccaagaat atcgttaaat ttttgtcccc ttttagtatc
     4621 ggttacaaaa tgccatacgc ctttgtagat aactttttca gaagcgggaa gttgtcggga
     4681 aggtttttca ccgtgataaa agatatagcc gtcgtcgcca gattttgcac tgcttgaccc
     4741 gttttctctt ttgatttcgc ttttcgcctg tttgtaaaac cagccggaat aaacatattt
     4801 aaaatcttta taatctgtta cttcgttttt tggttggttt gcaccgccat tagtgctgcc
     4861 gttttgatgg tttgattgca tgagataagg ggaagaatga atattattgc tgccatcgtc
     4921 tgtttttact tcatcaataa ccgatttctg tcgcttaggt aagtttttgg gatcgtcagg
     4981 taatcccgtc tcctcccaat cactctcttt cagtttaacc tcgtcttctt ttccccgcgg
     5041 caaccagaac cgccgcttga agcgcatcgc gaaaccgtat ccgccttggt ctttttgggc
     5101 tttaggcttt tcggaagaaa catcttgata ttttggcgcg ggacgcgggg cttcggtatc
     5161 gacagaatca agatcgaaac tgccgcctcc gcccaaacaa gcactcaaca aaaacaccgg
     5221 cagcaccata gcagcctgat tcaccaatgg attgttcata taaacccaat tcaattaaag
     5281 aatgataagg attattattt tatttatttt taacaaattt gcaaatactt ttttattttt
     5341 ttagggaata caccaaaatc ccgtcattcc cgcgcaggcg ggaatccagt tttttgagtt
     5401 tcagtcattt ccga
//
LOCUS       DAXHSF010000135         5343 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.135, whole genome shotgun sequence.
ACCESSION   DAXHSF010000135 DAXHSF010000000
VERSION     DAXHSF010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5343
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.135"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..1578
                     /locus_tag="ACFM8S_001655"
     CDS             <1..1578
                     /locus_tag="ACFM8S_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HFC6314703.1"
                     /translation="QEGEQSAAASAVSNDPQPQKQPAPQKDRLKPLPLADIRTFQAWL
                     KTAERENPRLLFLSRDDLMQHAAAHITEEQFPKFWQTADGKFKLSYRFEPHHPLDGVT
                     MTVPLTVLNRLHAPSLEWLVPGMLREKIQLLIKALPKQIRRICVPVPDFITKFLENNP
                     DRQAAIIPQLAHFIAKSASDMRILEQIDQDAWAAQDLPEHCYLNLRIIDDGGQELAGG
                     RKLHELQQQLGKAAATTFRDNTQEFERDNVTTWDIGILPESIKFARGKQQLTGYLGLQ
                     KEKDGRIALRLFDTTEAAEQAHRQGVIELMKLQLKEQVKDLNKGIQGFTQAAMLLKHI
                     NADTLRDDLTQAVCDRAFIGEDELPRNEKAFKEQIKRARSRLPAVKEALSRYLQETAA
                     AYAELNGKLGKHPLTHLLRLRLQTLLAAGFATRTPWAQWPRLPIYLKAMTLRLEKYSS
                     NPARDAAREADIQELEQMWQEKNDGLVKQGLPVSDDLTAFKWMIEELRVSLFAQELKT
                     PYPVSVKRLLKMWEDLN"
     gene            complement(1601..2023)
                     /locus_tag="ACFM8S_001656"
     CDS             complement(1601..2023)
                     /locus_tag="ACFM8S_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HFC6314704.1"
                     /translation="MTDIFTTSKRSFVMSKIHSKETKPEILVRKFLFSQGFRYRKNDK
                     RYAGKPDIVLPKYKTVVFIHGCFWYGHSCNKGHIPKSNTDFWLEKITKNCERDIKNET
                     ELEKIGFKVIVVWECELKNKAIGRERLNRLVREIKDAV"
     gene            complement(2027..3673)
                     /locus_tag="ACFM8S_001657"
     CDS             complement(2027..3673)
                     /locus_tag="ACFM8S_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02391 family protein"
                     /protein_id="HFC6314705.1"
                     /translation="MSEEKLKMSFEPTVIEHLGVKMYSHTVPAIAELIANAYDACATE
                     VEVRLFDKPEHKIVIKDNGIGMSFDEINDFYLRIGRNRREEKQASPCGRIPTGKKGLG
                     KLALFGLGNKIEISTIQGNERVTFTLDYAEIRRSKGIYQPEFRKESVESNIESGTTIT
                     LTELTKKQGYPLDNYVEHLSRLFDFPAQDFKIKVSLNGSEPKIIDGNLKYDLVTPQFE
                     WEYQDLATNISSLSSKFEQYEYSGLIQGKFITTEKPLKNNMKGITLFANGRMVNMPEF
                     FTDSESSHFYSYLTGWLNVDFIDNDDEDLIATDRTSLDWKHSKTSKLRVFLGEIVRAI
                     GQDWRKRRKKEKDDEVKGESGVDIEDWKNKLPEKEREPVEVILNRLEDSELTNKEQAE
                     VISALHSIIPEYPYYHWRHLHQDLHTACNDFYNEKKDYLSAAIEAVKVFEDKVQKQTG
                     LHSIDGRELIEQAFGSKNSILLLTNNKTKAEQNLEDGLEQLACGTWTGFRNPVQHELR
                     ANLSPSIFNDKDALDLISLVSYLLRKVEQTKKRSKVVSSK"
     gene            complement(3688..4743)
                     /locus_tag="ACFM8S_001658"
     CDS             complement(3688..4743)
                     /locus_tag="ACFM8S_001658"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC6314706.1"
                     /translation="MKNSKLKAVDFFCGGGGMSYGMQSAGIQVLAGIDYEPSCKETYE
                     ANINAKFIQADVFELQPETLEKELGLKKNDDDLILIGCSPCQYWSVIQTDKRKSEKSK
                     SLLLEFQRFVEYFNPGYVVVENVPGILSRMKESRLDNFIKLLEEKGFTVHFGIHNTAD
                     YGIPQSRKRFTLIANRITKEKLEPAKYSGKRLTVRDVLGMENGFPKIIAGHQDETDFM
                     HSCAGLSDINLKRLALIPKNGGNRLAFAHIPELQLECFIGKDNSFKDTFGRLWWDKPA
                     PTITTKFFSISNGRFAHPEEDRALSLREGATLQSFPRNYVFKAGSRDKIARLIGNAVP
                     PMYAEKIGRAIVDNIEC"
     gene            complement(5149..5343)
                     /locus_tag="ACFM8S_001659"
                     /pseudo
     CDS             complement(5149..5343)
                     /locus_tag="ACFM8S_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         1430 a         1405 c          998 g         1510 t
ORIGIN      
        1 caggaggggg aacagagtgc cgcagcttca gcggtttcaa acgaccctca accccaaaag
       61 cagccagcac cccaaaaaga ccgtctgaaa cccctccccc tcgccgacat ccgcaccttc
      121 caagcctggc tcaaaaccgc cgaacgcgag aacccgcgcc tgctgttcct cagccgcgac
      181 gatttgatgc aacacgccgc cgcacacatt accgaagaac agttccccaa attctggcaa
      241 accgcagacg gcaagttcaa actttcctac cgctttgagc cacaccatcc gctcgacggg
      301 gtgaccatga ccgtgccgct gaccgtcctc aaccgcctgc atgcgccgtc gctcgaatgg
      361 ctggtgcccg gtatgttgcg cgaaaaaatc cagctgctga ttaaagcact gccgaagcaa
      421 atccgccgca tttgcgtgcc cgtacccgat ttcatcacca aattcctcga aaacaatccc
      481 gaccgccaag cggccatcat tccccagttg gcgcacttta tcgccaaaag cgccagcgat
      541 atgcggattc tcgagcaaat cgaccaagac gcatgggcgg cgcaagactt gcccgaacac
      601 tgctatctga atctgcgcat tatcgacgac ggcggacaag agcttgccgg cggccgcaaa
      661 ctgcacgaat tgcaacaaca gctcggcaaa gccgccgcca ccaccttccg cgacaacacc
      721 caagaattcg agcgcgacaa cgtaaccaca tgggacatcg gcatcctgcc cgaatccatc
      781 aaattcgccc gcggcaaaca acagcttacc ggctatctcg gcctgcaaaa agaaaaagac
      841 ggccgcatcg ccctgcgcct atttgacacc accgaagccg ccgaacaggc acaccgtcaa
      901 ggtgtcatcg aattaatgaa gctgcaatta aaagagcagg tgaaagattt gaacaaaggc
      961 atccagggct tcacccaagc cgccatgctg ctcaaacaca tcaacgccga caccctgcgc
     1021 gacgacctca cccaagccgt ttgtgaccgc gcctttatcg gcgaagacga gctgccgcgc
     1081 aacgaaaaag ccttcaaaga acaaatcaaa cgcgcccgca gccgcctgcc cgccgtcaaa
     1141 gaagccctca gccgctacct gcaggaaacc gccgccgcct acgccgaact gaacggcaaa
     1201 ctcggcaaac acccattgac ccaccttcta agactacgcc tgcaaaccct gctcgccgcc
     1261 ggcttcgcca cccgcacccc gtgggcacaa tggccgcgcc tccccatcta cctcaaagcc
     1321 atgaccctgc gcctcgaaaa atacagcagc aaccccgccc gcgacgcagc ccgcgaagcc
     1381 gatatacagg aattggaaca aatgtggcag gaaaaaaacg acggtttggt gaaacaagga
     1441 ctacccgttt cagacgacct caccgcgttt aaatggatga tcgaagaatt gagggtgtcg
     1501 ctgttcgcgc aggaattgaa gacgccttat ccggtgtcgg tgaagcggtt gttgaagatg
     1561 tgggaggatc ttaattaaag gaagatgccg tctgattttt tcagacggca tcttttattt
     1621 ccctgaccaa ccgatttaat cgctccctac caatagcttt attttttaat tcgcactccc
     1681 aaacgacaat cactttgaaa ccgatctttt ccagctcggt ttcattttta atatcacgtt
     1741 cgcaattttt tgtgattttt tccaaccaaa aatccgtatt gcttttcggg atatgtcctt
     1801 tattacaaga atgcccgtac caaaaacagc cgtgtataaa cacgactgtt ttatatttcg
     1861 gcaaaacgat atccggtttg cctgcatagc gtttatcgtt tttccgataa cgaaaacctt
     1921 gggagaaaag aaatttcctt accaatattt ccggtttggt ttctttgcta tggatttttg
     1981 acatcacgaa agaacgtttg gatgtagtga aaatatcggt cataaattac ttggaagata
     2041 ccaccttcga tcgttttttt gtctgttcca cttttcttaa caaatacgaa accaagctta
     2101 ttaaatccaa agcatcctta tcattaaaaa tactaggaga taaatttgct cttaactcat
     2161 gttgtacagg atttctaaat ccagtccatg ttccgcatgc aagctgttca agaccatctt
     2221 ctaaattttg ttccgccttc gttttgttat tcgttaatag aagtatagaa tttttactcc
     2281 cgaaagcttg ttcaatcaat tctcttccgt ctatgctatg aagacctgtt tgcttctgaa
     2341 ccttatcttc gaaaacttta actgcctcga ttgcagcact taaataatcc ttcttttcat
     2401 tgtaaaaatc attacaggca gtatgcaaat cctgatgcaa atgccgccaa tgatagtaag
     2461 gatattctgg aataatacta tgtaaggcag agattacttc cgcttgctct ttatttgtaa
     2521 gctccgaatc ttccaaacga ttcaaaataa cttctactgg ttccctttcc ttttctggca
     2581 atttgttttt ccaatcttcg atatcaactc cgctttcgcc tttcacttcg tcatctttct
     2641 ctttttttct tctttttctc caatcttgcc cgatggctcg gacgatctcc ccaaggaaaa
     2701 cacgtaattt tgaggttttt gaatgcttcc agtctaatga tgtcctgtcg gtagcaatta
     2761 aatcttcatc gtcattatct ataaagtcca cattcagcca tccagtcaaa tatgaataga
     2821 aatggctgga ttcgctatca gtgaaaaact cgggcatatt taccattctg ccgttggcaa
     2881 acaaggtaat acctttcata ttattcttta aaggtttttc cgttgtaatg aacttacctt
     2941 gtattaatcc gctgtattca tactgttcga attttgaaga taacgatgaa atattggttg
     3001 ctaaatcctg gtattcccat tcgaattgtg gggtaacaag atcatatttt agatttccat
     3061 caatgatttt aggttcagag ccgttcaagc ttactttgat tttaaaatcc tgagccggaa
     3121 aatcaaacaa gcgggaaaga tgctctacat aattatctaa cggatatcct tgctttttcg
     3181 tcagttcggt taaagttatg gttgtcccgc tttcgatatt ggattcaaca gattcttttc
     3241 gaaactccgg ttgataaata cccttgcttc ttcgaatctc tgcataatcc aaagtaaaag
     3301 taaccctttc gtttccctgg atagtagaaa tttcaatttt gttgccaagc ccgaataatg
     3361 ccaatttacc aaggcctttt ttacccgttg gaattcttcc gcacggggaa gcttgttttt
     3421 cttcccttct gttccgaccg attctcaaat aaaaatcatt gatttcatcg aagctcattc
     3481 ctataccatt atctttgata acgattttat gctccggttt atcgaataac ctaacttcca
     3541 cttccgtagc acatgcatcg taggcattcg ctatcaactc ggcaatcgca ggaacagtgt
     3601 gcgaatacat ctttacaccc aaatgttcga ttacggttgg ctcgaaactc attttcaatt
     3661 tttcttcaga catgtttttc tccttttcta acattcgata ttatcaacaa ttgccctgcc
     3721 tattttttcc gcatacatcg gaggaacggc attaccaatc aaacgggcta tcttgtccct
     3781 gctgcccgct ttaaagacat agttacgagg gaacgattgc aatgttgcac cttcgcgcag
     3841 tgataacgcc ctatcttctt caggatgggc aaacctcccg ttggaaatac taaagaattt
     3901 ggtcgttatg gtaggtgcgg gcttatccca ccatagccta ccaaacgtgt ctttaaaact
     3961 attgtctttt ccgataaaac attccagctg taattcggga atatgcgcaa aagccaaacg
     4021 gtttcctccg tttttaggta tcaaagccaa tcgtttcaag ttgatatcag ataatcccgc
     4081 acagctatgc ataaaatccg tttcgtcttg gtgtcctgca ataattttgg gaaagccatt
     4141 ttccattcct aaaacatcgc gtaccgtaag ccgtttgccc gaatacttgg ctggttccag
     4201 cttttctttg gttattctgt ttgcaattaa cgtaaatctt ttacggcttt ggggaattcc
     4261 gtaatcagcc gtattgtgaa taccgaaatg gacggtaaat cctttttctt ccaataactt
     4321 tatgaaatta tcaagcctgc tttctttcat tctgctcaga attcccggta cgttctctac
     4381 aacaacatat ccgggattaa aatattctac aaaccgctga aactccaaaa gtaaactctt
     4441 ggatttttcc gattttcttt tgtctgtttg aataacactc caatattgac aaggactaca
     4501 acctataaga atcaaatcat catcattctt ttttaacccg agttcttttt ccaaggtttc
     4561 aggctgcaat tcaaaaacat cagcctgtat aaatttggca tttatattgg cttcatatgt
     4621 ttctttgcag ctgggttcgt aatctatccc ggctaaaacc tgaataccgg cggattgcat
     4681 tccatagctc attccacctc cgccacagaa aaaatcaacc gcctttaact tactgttttt
     4741 catttctatt taaataatta gactagtgag aactaacttt atataacata taatttattg
     4801 cttatcaaaa ataatttgag attttattaa aattcatgca tctaaataag cagaacttaa
     4861 tttcctccaa acgagcggat gtcttcaaat gcacacttca cagaaaggtc gtctgaaacc
     4921 cattttcaga cgaccttttt ccacttccca aaacaagaat tcccaccccc tccccaaacg
     4981 tcgcccgaac cagtaaactc cccatatttc aacaacttct tggaagccaa ccatgccccg
     5041 tatccaccct cctcacctgt tcccgcccca tatcgccgaa cgcggccttt tgtattttca
     5101 gcagggcaag gttcgcgatg tccgaaaaac ttccgccggg cgttatcggg cctggcgctg
     5161 tttgctgccg gcggtctgaa taatggtggc gtcaacgacg gcagcggatg ctttctctat
     5221 ttttaagcct ttttcggtca gttggcggtt aatcagtttg agcaattcgg acagggtgtc
     5281 gtcttgcgcc agccagttgc ggtagcggca taaggtgctg taatcgggga tgctcagttc
     5341 gtc
//
LOCUS       DAXHSF010000136         5331 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.136, whole genome shotgun sequence.
ACCESSION   DAXHSF010000136 DAXHSF010000000
VERSION     DAXHSF010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5331
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.136"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            304..1737
                     /locus_tag="ACFM8S_001660"
     CDS             304..1737
                     /locus_tag="ACFM8S_001660"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFC6314707.1"
                     /translation="MNEYSQLIKHPDVSLSPISDGIGVDNPATGEILAYVRNTGSDKL
                     KNLIQKAAAAQKLWAAKTALERADILWRWYFLVKENKEALARLMTMEQGKSLTESRGE
                     IDYAASFVRWFAEEARRIDGDVLTSVKASQKLVVLKQPVGVTAAITPWNFPSAMIARK
                     AAPALAVGCAMIVKPASLTPLSAYALVVLAYEAGVPQDLLPVVSGRASEIGREFATNP
                     IIRKISFTGSTEVGAKIFADSAADIKKLSLELGGNAPFIVFDDADLDKAVEGALASKF
                     RNSGQTCVCTNRVYAQSGIYDEFCRKLSEKVAELKLGNGLEDGVNQGPLIEEKAVEKV
                     EQHIADALAKGAVCLTGGKRSALGGTFFEPTVLSGVTAQMAVAREETFGPLCPVFRFE
                     TEVEVIEAANNTEYGLAAYLFTADTARQWRVGEALEYGMVGINTGLISNEAAPFGGVK
                     RSGLGREGSKYGADEYLELKYLCMDVG"
     gene            1847..2635
                     /locus_tag="ACFM8S_001661"
     CDS             1847..2635
                     /locus_tag="ACFM8S_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimb protein"
                     /protein_id="HFC6314708.1"
                     /translation="MMENGKTVPRWRFALKSAGWHLLISLSVAGLAALLVFKVWYPYP
                     YAELTGGLSLYQLVVAVDIVCGPLLTLILASPKKKTKARMVDFSMVGIIQLAALVYGL
                     HSVSLARPVVEAFEKDRINVVTAAEVAVEDLHKAPEGLQSLSWFGIRRIALKEPEDAD
                     EKNKTLDLSLKGIEPSMRPDRWLPYSDKEAEEIRKHLKPLKVLADARKTTVADILKQA
                     GLAEGEELYYLPFTSSRQKEWIVITDKEGNTKGYAPIDGFIITP"
     gene            3003..4559
                     /locus_tag="ACFM8S_001662"
     CDS             3003..4559
                     /locus_tag="ACFM8S_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HFC6314709.1"
                     /translation="MDFSWLAEPHTWIGFATLLVLEVVLGIDNLVFVAILANKVQPAR
                     RDRARIIGLGLAVVIRIIMLAFMAHIITLTEPLFQIGGLAVSGKDMIMLAGGIFLLYK
                     ATTELHERLEGHNRFTVADSQKKHAPFWGVVAQILILDAVFSIDSVITAVAMVDHIVV
                     AMGAVVVAMAVMISASKLLTEFVDRHPTVVMLCLGFLLMIGFSLIAEAFHFHIPKGYL
                     YAAIGFSILIELFNQISQRNSRKNDYIGSSWRKRTAENVLGMMGIRESVLADAGGESV
                     DDAHFEENEKSMIRSVLTLAERPIMGVMIPRRDIERLDISQSREEQCAQLQNTPYSRL
                     LVVGKAGVDEPLGYINKKDLLSQLLETGGLDIQTALRQPLVLPDSTTALGAIELFRQS
                     SADYALVVDEFGAVLGMVTMKDLLETIAGEFPEEFEREEEPAVQGNPDESLTVEGALE
                     YVELAPQLNLPQQEEDADFHTVAGLIMEELQTIPDVGDFADFHGWRFEVVEKEGQRIE
                     RVKITKLPEE"
     gene            4576..5322
                     /gene="surE"
                     /locus_tag="ACFM8S_001663"
     CDS             4576..5322
                     /gene="surE"
                     /locus_tag="ACFM8S_001663"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFC6314710.1"
                     /translation="MNVLISNDDGYLSEGIAVLARVTAEFANVRVVAPERDRSGVSNS
                     LTLERPLQLKQAQNGFYYVNGTPTDCIHIGQSVFSDFQADFVFSGINRGANMGDDTLY
                     SGTVAAATEAYLMGIPAVAFSLNDASGRYWATAEQALWTLLAHFFKNPPQSPILWNIN
                     IPAVAPEDVRGIKIARLGRRHHGQNVIPARNPRGEQIYWIGPVGEVSDREEGTDFGEC
                     GAGFITVTPLQIDLTAYPDMAETAAFWHTD"
BASE COUNT         1237 a         1355 c         1529 g         1210 t
ORIGIN      
        1 gtttggattt ttgccatact ctaccacacg ttgcaactgc aatctttgct ccttatatat
       61 tgcaccccat caaagggctg cattactttt ctttctaaag attacttttg tataaaaagt
      121 atttttctta agcgataggg attacgtccg cgccatattc agacatcgcc cggatgttgc
      181 gctgcccgat cggatgcttc agacggcatc ggaagggttt gcggttttgg aatatgaaga
      241 tgataatgtc cgcgagattg acggcatttg aaagcggtta tcctacatac cggaggacac
      301 gaaatgaacg aatattccca attaatcaag catcccgatg tttccctttc cccgatttca
      361 gacggcatcg gggtcgacaa tccggcaacg ggcgaaattt tggcgtatgt ccgcaacacg
      421 ggttcggaca aactgaaaaa cctgattcaa aaggcggcgg cagcgcaaaa attatgggcg
      481 gcgaaaactg ctttggaacg cgccgacata ttgtggcgtt ggtatttttt agttaaagaa
      541 aacaaggaag cactggcgcg cctgatgacg atggagcagg gcaaaagcct gaccgagtcg
      601 cgcggcgaaa tcgattatgc ggcttcgttt gtgcgctggt ttgcggaaga ggcgcggcgg
      661 attgacggcg atgtgctgac aagtgtgaaa gcgtcgcaaa aactggtcgt gttgaaacag
      721 cctgtcggcg ttaccgccgc gattacgccg tggaacttcc catctgcaat gattgcgcgc
      781 aaggctgcgc ctgctttggc ggtgggctgc gcgatgatcg tcaaacccgc atcgctcacg
      841 cctttgagtg cgtatgcgtt ggtcgtgctg gcttatgaag cgggcgtacc acaggatttg
      901 ttgcccgtcg tcagcggtcg cgcatcggaa atcggccgtg aatttgccac gaacccgatt
      961 atccgcaaaa tcagcttcac cggctctacc gaagtcggtg cgaaaatttt tgccgacagc
     1021 gcggcggaca ttaaaaaact cagtttggag ctgggcggca acgcgccgtt tatcgtgttt
     1081 gacgatgccg atttggacaa agccgtcgaa ggtgcgctcg ccagcaagtt ccgcaacagc
     1141 ggtcagacct gcgtctgcac caaccgcgtt tacgcccaat ccggcattta cgacgaattt
     1201 tgtcgcaaat tgagtgaaaa agtagccgag ctcaaattgg gcaacggctt ggaggatggc
     1261 gtcaaccaag ggccgctgat tgaggaaaaa gcggtagaaa aagtcgagca gcacattgct
     1321 gacgcgctcg ctaaaggcgc ggtctgtctg accggcggca aacgcagcgc attgggcgga
     1381 acgttttttg agccgaccgt cttaagcggc gtaacggcgc aaatggcggt ggctcgcgaa
     1441 gaaaccttcg ggcctttgtg tccggtattc cgttttgaaa ccgaagtcga ggtcatcgag
     1501 gctgcgaaca atacggaata cggtttggcg gcctaccttt tcaccgccga caccgcccgc
     1561 caatggcgcg tcggcgaagc cttggaatac ggtatggtcg gcatcaatac gggcttaatc
     1621 agcaatgaag cggcaccgtt cggcggcgtg aaacgcagcg gtttgggacg tgaaggcagc
     1681 aaatacggtg cggacgaata tctggaattg aaatatctgt gtatggatgt cgggtgacgg
     1741 atgccgtctg aacggcaggg tttcagacgg tgccgcattt taagcagtct ctatctgttg
     1801 tacaatgcgc cctgttttta cggttatttt tgatgtgaat taagatatga tggaaaacgg
     1861 aaaaacagtc ccgagatggc gttttgcctt gaaaagtgcg ggctggcacc tcttaatcag
     1921 cctgtcggtt gcagggctgg cggcattgct ggtttttaag gtttggtatc cttatcctta
     1981 tgccgagctg acgggagggc tgtcgcttta tcagctggtg gtggctgtcg atattgtatg
     2041 tggtccgctg ctgactttaa ttttggcaag cccgaagaaa aagacaaagg cacgcatggt
     2101 cgatttttcc atggtcggca tcatccagct ggcggctttg gtgtacggtc tgcacagcgt
     2161 ttctttggcg cgtcccgtgg tggaggcgtt tgaaaaagac aggattaatg ttgtgaccgc
     2221 cgccgaagtg gcggttgaag acctgcacaa agcgccggaa gggctgcaaa gcctgtcgtg
     2281 gttcggcatc cgccgcattg cattgaaaga acctgaggat gcggatgaga agaacaagac
     2341 gctggatttg tccctgaaag gtatcgagcc gagtatgcgt cccgaccggt ggctgccgta
     2401 ttccgacaag gaagcagaag aaatccgcaa acatctgaaa ccgctgaaag tcttggcgga
     2461 tgcgagaaaa acgacggttg cggacattct gaaacaggca ggtctcgccg aaggggagga
     2521 gctgtattac ctgccgttta ccagcagcag gcagaaagag tggatagtca ttaccgataa
     2581 agagggcaac accaaaggct acgcgccgat agacggcttc atcatcaccc cttaagcgtt
     2641 gggactccgt ccgcactcga acatccgttc ttcgcggcgg tagaatcaga ctgtatttga
     2701 gaggggcgca acccctcaat cagggcaaca cataggggaa ggctttatgt gccgtccgtg
     2761 tgttgaaaca tttcaaaata agctcatgcc gtccgaacat cctttcagac ggtatggcat
     2821 tttgccattg ccccgataag ctgttaaact atttaaatta tttcgaccga aggtacacgc
     2881 acatgaaaga ccccgagcag agcagcaagc ccgcccgccg ttttttgtgc gtaccgtctg
     2941 ttcaggcagc ttgagcgctt caggctgcct tttcgcgttt gaacttgagg aatacgaaaa
     3001 tcatggattt cagttggttg gcagaaccgc atacctggat aggttttgcc acgcttttgg
     3061 tgttggaagt cgtattgggg atagacaacc ttgtctttgt ggcgattttg gcaaacaagg
     3121 tccagcccgc acggcgcgac cgcgcacgga ttatcgggct ggggctggca gtcgtcatcc
     3181 gcatcattat gcttgctttt atggcgcaca tcatcacgct gaccgagccg ctgttccaaa
     3241 tcggcggcct cgccgtttcc ggcaaggaca tgattatgct cgcgggcggt attttcctgc
     3301 tttacaaagc caccaccgaa ctgcacgaac gcctcgaagg gcacaaccgg tttaccgttg
     3361 ccgacagcca gaaaaaacac gcgccgtttt ggggcgtggt cgcgcaaatc ctgatactgg
     3421 atgctgtgtt ttccatcgat tcggtcatca ccgccgtagc gatggtcgat cacatcgttg
     3481 tggcgatggg cgcggtcgtc gtcgcgatgg cggtgatgat ttccgccagc aaactcttga
     3541 ccgaatttgt agacaggcat cccaccgtcg tgatgctctg ccttggtttt ttgttgatga
     3601 tcggtttcag cctgattgcc gaagccttcc atttccacat tcccaaaggc tacctctacg
     3661 ccgccatcgg cttctcgatt ttaatcgaac tgtttaacca gatttcccag cgcaacagcc
     3721 gcaaaaacga ctacatcggc agctcgtggc gcaagcgcac cgccgaaaac gtcttgggta
     3781 tgatgggtat acgcgaaagc gtgcttgccg acgcgggcgg cgaatccgtg gatgacgcgc
     3841 attttgaaga aaacgaaaaa tcgatgatac gcagcgtgct gacgcttgcc gaacgcccga
     3901 ttatgggggt aatgatccca cgccgcgaca tcgaacggct ggacatttcc caaagccgcg
     3961 aagaacagtg tgcccaactg caaaacacgc cttacagccg cctgctcgtt gtcggtaagg
     4021 cgggcgtgga cgaacctttg ggctacatca acaaaaaaga cctgctgtcc caactgctgg
     4081 aaacaggcgg tctcgacatt cagacggcat tgcgccagcc gctcgttttg cccgacagca
     4141 ccaccgcgct gggcgcaatc gaactcttcc gccaaagcag cgcggattat gctttggtgg
     4201 tggacgagtt cggcgcggta ttgggcatgg taaccatgaa agacctgctc gaaaccatcg
     4261 caggcgagtt ccccgaagaa tttgagcgcg aagaagaacc agccgttcag gggaatcccg
     4321 atgaaagcct gacggtggaa ggcgcgttgg aatatgtgga actcgcaccg caactcaacc
     4381 tgccgcagca ggaggaagat gccgatttcc atacggttgc cgggctgatt atggaagaat
     4441 tgcaaaccat ccccgatgtc ggtgattttg ccgatttcca cggctggcgg tttgaagtgg
     4501 tcgaaaaaga agggcagcgc atcgagcggg tcaaaatcac caaattgccc gaagaataag
     4561 tattcaggat agaaaatgaa cgttttgatt tccaacgacg acggctacct ctccgaaggc
     4621 atcgccgttt tggcgcgcgt tacggcggaa tttgccaacg tcagggtggt tgcgcccgaa
     4681 cgcgacagga gcggggtcag caattcgctg acgctggaac gccctttgca gttgaaacag
     4741 gcgcaaaacg ggttctacta tgtcaacggc acgccgaccg actgcatcca catcgggcag
     4801 tctgtatttt cggattttca ggccgatttt gtcttttcgg gcatcaaccg gggcgcgaat
     4861 atgggggacg acacgcttta ttcggggacg gttgcggcgg caaccgaagc ctaccttatg
     4921 ggcatacccg ctgtggcgtt ttccttaaac gacgcttccg gacgctattg ggcgaccgca
     4981 gaacaggcac tgtggacatt gttggcgcat tttttcaaaa accccccgca gtcccctatt
     5041 ttgtggaaca tcaatatccc cgccgtcgcg ccggaagatg tgcggggcat taaaatcgcc
     5101 cgtttgggca ggaggcacca cggtcagaac gtcatccccg cgcgcaatcc gcgcggcgaa
     5161 cagatttatt ggatagggcc ggtcggcgaa gtttccgatc gggaagaggg aacggatttc
     5221 ggtgaatgcg gcgcaggttt cattaccgta acgccgctgc aaatcgacct gaccgcctat
     5281 ccggacatgg cggaaacagc ggcgttctgg catacggact gaccgtttca t
//
LOCUS       DAXHSF010000137         5327 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.137, whole genome shotgun sequence.
ACCESSION   DAXHSF010000137 DAXHSF010000000
VERSION     DAXHSF010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.137"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            53..1482
                     /locus_tag="ACFM8S_001664"
                     /pseudo
     CDS             53..1482
                     /locus_tag="ACFM8S_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174056.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            1496..1630
                     /locus_tag="ACFM8S_001665"
     CDS             1496..1630
                     /locus_tag="ACFM8S_001665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314711.1"
                     /translation="MPSEGHTSFRQHCLDARHNTRNAEIRTGLPTSVGCFRAGGVPSA
                     "
     gene            1726..2382
                     /gene="cmk"
                     /locus_tag="ACFM8S_001666"
     CDS             1726..2382
                     /gene="cmk"
                     /locus_tag="ACFM8S_001666"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFC6314712.1"
                     /translation="MNRQKVIAIDGPGASGKGTVAARVAAALGYDYLDTGALYRLTAL
                     YAQKQGVGWHDEENVSELAKKLPAVFSGSRILLGGEDVSDGIRTEAIGMGASAVAQLP
                     KVRAALLQRQRDFLTEKGLVADGRDTGSVVFPQAELKIFLTAESKIRAERRAKQIGIP
                     CEGLAFERILSDIEARDEADRNRKVAPLKQQPDALLLDTSRLTIEETVKKVLDWYREV
                     "
     gene            2538..4223
                     /gene="rpsA"
                     /locus_tag="ACFM8S_001667"
     CDS             2538..4223
                     /gene="rpsA"
                     /locus_tag="ACFM8S_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFC6314713.1"
                     /translation="MSMENFAQLLEESFTLQEMNPGEVITAEVVAIDQNFVTVNAGLK
                     SESLIDVAEFKNAQGEIEVKVGDFVTVTIESVENGFGETKLSREKAKRAADWIALEEA
                     MENGNILSGIINGKVKGGLTVMISSIRAFLPGSLVDVRPVKDTSHFEGKEIEFKVIKL
                     DKKRNNVVVSRRAVLEATLGEERKALLENLQEGSVIKGIVKNITDYGAFVDLGGIDGL
                     LHITDLAWRRVKHPSEVLEVGQEVEAKVLKFDQDKQRVSLGMKQLGEDPWSGLTRRYP
                     QGTRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVQLGDEVEVMIL
                     EIDESRRRISLGMKQCQANPWEEFAANHNKGDKISGAVKSITDFGVFVGLPGGIDGLV
                     HLSDLSWTESGEEAVRKYKKGEEVEAVVLAIDVEKERISLGIKQLEGDPFGNFISVND
                     KGSLVKGTVKSVDAKGAVVALSDEVEGYLPASEFAADRVEDLTTKLKEGDEVEAVIVT
                     VDRKNRSIKLSVKAKDAKESREALNSVNAAANANAGTTSLGDLLKAKLSGEQE"
     gene            4234..4548
                     /locus_tag="ACFM8S_001668"
     CDS             4234..4548
                     /locus_tag="ACFM8S_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFC6314714.1"
                     /translation="MTKSELMVRLAEVFAAKNGTHLLAKDVEYSVKVLVDTMTRSLAR
                     GQRIEIRGFGSFDLNHRPARIGRNPKTGERVEVPEKHVPHFKPGKELRERVDLALKEN
                     AN"
     gene            complement(4898..5305)
                     /locus_tag="ACFM8S_001669"
     CDS             complement(4898..5305)
                     /locus_tag="ACFM8S_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFC6314715.1"
                     /translation="MTYTTAKAAEKIGISAHTLRFYDKEGLLPNIGRDEYGNRCFTDN
                     DLQWLGLLQCLKNTGMSLKDIKRFAECTVIGDDTIEERLSLFENQIENVKCQIAELKR
                     YLDLLEYKLAFYQKAKALGSVKAVNLPQIPETA"
BASE COUNT         1390 a         1374 c         1263 g         1300 t
ORIGIN      
        1 tttgtggttt ttttatttta aaataaaatt taatttacca acaggaaccc ccatgaaacg
       61 acaccccacc ttacagcccc tatctcgcca gcaccatttg ggcttggtca tcgccaacaa
      121 agccaaatcc gtcaccgatg atgacaaact tacccaccat caagccttta tcctgaccgt
      181 tgccggcgcg actgtcggtt tcggcgcgac gtggcgtttc ccgtatttgg tcggtgaaaa
      241 cggtggtgcg cgtatgtgtt tttattctgt atcgcgatgc tggttatcgg catcccgatg
      301 attttggtgg aaaatgtcat cggacggcgc aaaggcgtga acgcgctgga tgcgttcggc
      361 ggcccgatga acggcaaacc cattgccaaa atttggaaac tggtcggctg gatgggctgc
      421 tcggcgcgtt cggcatcatg gcttattaca tggtactcgg cggctgggta atcagctata
      481 tcgttaatat tattggagga aatttgaata tttccagccc cgtcgacggt gtggttacaa
      541 aaggcttctt tgccgaacac attgaaacag cccttgggaa attgcgtttt atacgctgct
      601 ttttgtcgcc gtgaaccaat ggattttggt caaaggcgtt atcggcggca ttgaaaaagc
      661 ggcaaaatag ctgatgccgc tgctgttttt gttcctaatc gcgatggtcg tccgcaacgt
      721 tacccttccg ggcgcaatgg aaggggttgc tttctatctg aaacctgatt tcagcaagat
      781 taccgccgaa ctgttcgtct tcgttttggg gcaggtattt tttgccctga gcttgggttt
      841 cggcgtgatg attaccttgt ccagctattt ggataaaaac gaaaatctgg ttcagacggc
      901 agttatcacg gcaattacca ataccatcat cgccatactt gcgggcttta tgattttccc
      961 gtcgctcttc agcttcggcg ttgcccccga ttccggcccg actttggtgt tccaaagctt
     1021 gccgattgtg ttctcacata tgtgggcggg atctgtgttc gccgtgattt tcttctcgct
     1081 gctcctgatt gccgcgttga caacttcgct gaccatttat gaagtgttga ttacgaccat
     1141 tcaggaaaaa accaaaatcc gccgtaccgc cgcgattacg attgtattgg ctgccatctt
     1201 cattttcggc aacatcccgt ccattctgag ctatggtccg tggaaagacg ttccgtgttc
     1261 ggcaaaaata ttttcgatgc cttcgactac atcagcggca acatcttgtt tatgctgacc
     1321 gcgctcggtt ccgcgctgtt tgccggtttt gtgatgaagg acgaagcgaa ggacgaatta
     1381 ctttataaag gcaatcatac gacggtcaat atctggtttg cctatgtgaa atacctcgtg
     1441 ccgctggtga ttctgctgat tttcgtcagc aacctgttct aatccgcagc aatcgatgcc
     1501 gtctgaaggt catacctctt tcagacagca ttgccttgat gcccgccaca atacccgcaa
     1561 cgccgaaatc cgaaccggct tgccgacttc tgtcggatgt ttccgggcag gcggcgttcc
     1621 gtctgcttag acaatcgtcc tttaaaacag gtagaatccg ccccaatggg aaatacatcc
     1681 ttcagacggc aaaacccata ccccaaacca tcaggaatcc cccttatgaa cagacaaaaa
     1741 gtcatcgcca tcgacggccc gggcgcatcg ggcaaaggca cggttgccgc ccgcgttgcc
     1801 gccgcattgg gatacgatta tctcgatacc ggcgcactct accgcctgac tgccctatat
     1861 gcacaaaaac aaggcgtggg atggcacgat gaagaaaacg tttccgaact ggcaaaaaaa
     1921 ctgcccgccg tattttcagg cagccgcatc ctgctcggcg gcgaagacgt ttcagacggc
     1981 atccggacag aagccatcgg catgggcgca tccgcagtcg cacagttgcc taaagtccgc
     2041 gccgccctgc tgcaacgcca acgcgatttt ctgaccgaaa aaggactggt tgccgacgga
     2101 cgggacaccg gatcggtcgt cttcccccaa gccgaactta aaatcttcct gacggcagaa
     2161 tccaaaatcc gtgccgaacg ccgcgccaaa caaatcggca tcccctgcga aggtttggca
     2221 ttcgagcgca tcctgtccga catcgaagcc agagacgagg cagaccgaaa ccgcaaagtt
     2281 gcccccctga aacaacagcc cgatgccctg cttttggaca caagccgcct gactatagaa
     2341 gaaactgtaa aaaaagtgct tgattggtat cgtgaagttt aaattttcag gtataatcgc
     2401 acaaattacg tttcagacgg cataaaaatc ccccatatgc cgtctgaaac cttatgtacc
     2461 cgtctgccct gccaaggacg gcagatatcc accaacccac cccgcacccc ttggcggtgt
     2521 accgaaaaga gttatatatg tctatggaaa attttgctca gctgttggaa gaaagcttta
     2581 ccctgcaaga aatgaaccca ggtgaggtga ttaccgctga agtagtggca atcgaccaaa
     2641 acttcgttac cgtaaacgca ggtctgaaat cagaatccct gattgatgta gctgaattca
     2701 aaaacgctca aggcgaaatt gaagttaaag tcggcgactt cgttaccgtt accatcgaat
     2761 ccgtcgaaaa cggcttcggc gaaaccaaac tgtcccgcga aaaagccaaa cgcgctgccg
     2821 attggattgc cctggaagaa gccatggaaa acggcaacat cctgtccggc atcatcaacg
     2881 gcaaagtcaa aggcggcctg accgttatga ttagcagcat ccgcgcattc ctgccgggtt
     2941 ctttggtcga cgtacgtcct gtaaaagaca cttctcactt cgaaggtaaa gaaattgaat
     3001 tcaaagtgat caaactggac aaaaaacgca acaacgttgt tgtgtcccgc cgcgccgttc
     3061 tggaagctac cttgggtgaa gaacgcaaag ccctgctgga aaacctgcaa gaaggctccg
     3121 tcatcaaagg catcgttaaa aacattaccg attacggtgc attcgttgac ttgggcggca
     3181 tcgatggtct gctgcacatc accgacttgg catggcgccg tgtgaaacac ccgagcgaag
     3241 ttctggaagt cggtcaagaa gtagaagcta aagtcctgaa attcgaccaa gacaaacaac
     3301 gcgtttctct gggtatgaaa caactgggcg aagatccttg gagcggcctg acccgtcgtt
     3361 acccgcaagg tacccgcctg ttcggtaaag tatccaacct gaccgactac ggtgcgttcg
     3421 ttgaaatcga acaaggcatc gaaggcttgg tgcacgtttc cgaaatggat tggaccaaca
     3481 aaaatgtaca cccgagcaaa gttgtacaat tgggcgacga agtcgaagta atgatcctcg
     3541 aaatcgatga aagccgccgg cgtatctctt tgggtatgaa acaatgccaa gccaatcctt
     3601 gggaagaatt tgccgccaac cacaacaaag gcgacaaaat ctccggcgcg gttaaatcca
     3661 ttaccgattt cggcgtattc gtcggtctgc ctggcggcat cgacggtttg gttcacctgt
     3721 ccgacctgtc ctggaccgaa tccggcgaag aagccgtacg caaatacaaa aaaggcgaag
     3781 aagtcgaagc cgtcgtattg gcaatcgacg tggaaaaaga acgcatctcc ttgggtatca
     3841 aacaactgga aggcgatccg ttcggcaact tcatcagcgt gaacgacaaa ggttctttgg
     3901 ttaaaggcac tgtgaaatct gtcgatgcga aaggcgccgt tgtcgccctg tctgacgaag
     3961 tagaaggcta cctgcctgct tccgaatttg cagccgaccg cgttgaagat ttgaccacca
     4021 aactgaaaga aggcgacgaa gttgaagccg tcatcgttac cgttgaccgc aaaaaccgca
     4081 gcatcaaact ttccgttaaa gccaaagatg ccaaagaaag ccgcgaagca ctgaactccg
     4141 tcaatgccgc cgccaatgcg aatgccggca ccaccagctt gggcgacctg ctgaaagcca
     4201 aactctccgg cgaacaagaa taaggttgca gacatgacaa agtctgagtt aatggttcgt
     4261 ttggcagaag tgtttgccgc caaaaacggc acgcatcttc tggcaaaaga cgtagagtac
     4321 agcgtaaaag tcttggttga caccatgact agatcgcttg cccgaggtca acgcatcgaa
     4381 atccgcggtt tcggcagctt cgatttgaac catcgtcctg cccgcatcgg tcgcaatccc
     4441 aaaaccggcg agcgtgtgga agtacctgaa aaacatgtac cccacttcaa gcccggtaaa
     4501 gaattgcgcg agcgggtcga cttggcttta aaagaaaatg ccaattaaac cttagcatca
     4561 aaacgccgct gttacgcggc gttttttctg tggtttaact tcatccgctg cttcaatacc
     4621 ttgagccaag caagcaacgg attagagcgt ggatttttta tagtggatta acaaaaacca
     4681 gtacggcgtt gcctcgcctt agctcaaaga gaacaattct ctaaggtgct gaagcaccaa
     4741 gtgaatcgat tccgtactat ttgcactgtc tgcggcttcg tcgccttgtc ctgatttttg
     4801 ttaatccact atatcattgc ttacaatccg ctttttaaac aacaaatttt tgatttctat
     4861 tacgaacagg acaaaaatcc tgcttattgc actaaaacta agccgtttca ggaatttgcg
     4921 gcaaatttac ggcttttacc gaacctaatg ctttcgcttt ttggtaaaac gccaatttgt
     4981 attcaagcaa atctaaatag cgttttaatt cggcaatttg acacttcaca ttttctattt
     5041 gattttcaaa caaggaaagg cgttcttcaa tggtatcgtc gccaatgacg gtacattccg
     5101 caaagcgttt gatgtctttt aagctcattc ccgtattttt caagcattgc aataagccca
     5161 accattgcaa atcgttatcg gtaaaacagc ggttaccgta ttcatcacgt ccgatattag
     5221 gcaacaagcc ttctttgtcg taaaaacgta gggtgtgggc ggagatgcct attttttcgg
     5281 cggctttggc agtggtataa gtcattttcc ctccttctaa acaaaaa
//
LOCUS       DAXHSF010000138         5318 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.138, whole genome shotgun sequence.
ACCESSION   DAXHSF010000138 DAXHSF010000000
VERSION     DAXHSF010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5318
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.138"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            164..325
                     /locus_tag="ACFM8S_001670"
     CDS             164..325
                     /locus_tag="ACFM8S_001670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314716.1"
                     /translation="MPAPATIRQIPLFFPNRLQVSPIRRVMPPPPFKGNARVNGFAVG
                     KAAGYRYVS"
     gene            432..956
                     /gene="nspA"
                     /locus_tag="ACFM8S_001671"
     CDS             432..956
                     /gene="nspA"
                     /locus_tag="ACFM8S_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein NspA"
                     /protein_id="HFC6314717.1"
                     /translation="MKKALATLIALAIPAAALAEGASGFYVQADAAHAKASSSLGSAK
                     GFSPRISAGYRINDLRFAVDYTRYKNYKAPSTDFKLYSIGASAIYDFDTQSPVKPYLG
                     ARLSLNRASVDLGGSDSFSQTSTGLGVLAGVSYAVTPNVDLDAGYRYNYIGKVNTVKN
                     VRSGELSAGVRVKF"
     gene            1092..1481
                     /locus_tag="ACFM8S_001672"
     CDS             1092..1481
                     /locus_tag="ACFM8S_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFC6314718.1"
                     /translation="MSKTIIHTDKAPAAIGAYSQAVRAGDTVYMSGQIPLDPATMTVV
                     GNGDFRAEARQVFQNLQAVAEAAGGTLADIVKLNAYLTDLGNFAVFNEVMAEFIAEPF
                     PARAAVGVASLPKGVQVEAEAVLVLNA"
     gene            1559..2389
                     /locus_tag="ACFM8S_001673"
     CDS             1559..2389
                     /locus_tag="ACFM8S_001673"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HFC6314719.1"
                     /translation="MAHYAIGDIQGCFDELTALLGKIGFNHGTDTLWLTGDIVNRGPK
                     SLETLQFCIRHENSVQIVLGNHDLHLLAVGCGEGAPKRSDTIEPILKHPDGKKMLDWL
                     RAQPLLIREGSRVMVHAGILPQWRIAKAESLAREAEAELRGKKYVKFFSKMYGNKPAA
                     WDEGLEGYARLRFIVNAFTRMRALTFKNELDFDYKSTVKKMPPYLRPWFKAPDRQNLD
                     RTIIFGHWSSLGYTNADNVISLDTGALWGGQLTAVNLETEEITQVQAAGGIDWKSFAK
                     "
     gene            2404..3861
                     /locus_tag="ACFM8S_001674"
     CDS             2404..3861
                     /locus_tag="ACFM8S_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFC6314720.1"
                     /translation="MHKILPIAHVLSRLGMLFSFILLIPAALSYAFSDGAYTAFATTA
                     TVTLSGSCIVRLATLRFRRELRPRDGFTLVLMLWLAFAAMAAMPMYLYFPNMGFTDAF
                     FESMSGLTTTGATVIPHVDGLAPSVNFWRHMLNWLGGMGIIVLAVAILPMLGVGGTQL
                     FKAEIPGIDKESKMSPRISQVAKKLWFGYTLITILAAACLHFAGMGWFDAVCHAMATL
                     SLGGFSTHDASIAYYNSPLIEAVIIVFTIVGGINFANHFAALNSRSLKTYWKDEECRT
                     MLLLLSGSILAAALYLWHTGYYAGFTESLRYTAFNFVSIGLANGLANTDFAQWPLLIS
                     LWMFFLANILANSGSTGGGIKTIRALVLFKFSLREMMVLLHPKAVRTVKISGKAIPDR
                     LALTVMSFIFIYFMTVVLFSFLLMASGMEFTTAFTAVIACITNAGPGLGEVGPAGNYA
                     GLDVMQKWICVTAMLLGRLEIFTVFILFTPAYWKK"
     gene            complement(3902..4291)
                     /locus_tag="ACFM8S_001675"
     CDS             complement(3902..4291)
                     /locus_tag="ACFM8S_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314721.1"
                     /translation="MWKISKENCEDLGFAIVCMFYDAINLSEFKLWLDIVVRDTPIDT
                     IPLYIFDLIDFDKSIGEIYDVIGVVNYGYISNDQKNALTGIAFLRGIDVYDPPISKEK
                     ALKALEKYPEIYQRFQHFFPFVELPLF"
     gene            complement(4473..4874)
                     /locus_tag="ACFM8S_001676"
     CDS             complement(4473..4874)
                     /locus_tag="ACFM8S_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314722.1"
                     /translation="MKIKPLQFSNNSRRFYVDKICVTEQDVNILASDRNYELNIEIFI
                     DNIIHFQITDESYKVKFSEYLFENKEKNDWDRNPAINYFFEIIDDSYMDWLKEESFDF
                     FEKKYYKAYIFFFSDSVIEVISSTEPVFYSK"
     gene            complement(4982..5161)
                     /locus_tag="ACFM8S_001677"
     CDS             complement(4982..5161)
                     /locus_tag="ACFM8S_001677"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314723.1"
                     /translation="MPSETVFNFQATFPLFETGYWELSSSKILHLLLPRQHLGQNGRR
                     LKSKHCHQPFEDRME"
     gene            complement(5180..>5318)
                     /locus_tag="ACFM8S_001678"
     CDS             complement(5180..>5318)
                     /locus_tag="ACFM8S_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Imm41 family immunity protein"
                     /protein_id="HFC6314724.1"
                     /translation="TIKSSPWLPKSIKINERYERFRVMLRYIFTEKDIVNVRFDYYNK
                     K"
BASE COUNT         1309 a         1577 c         1137 g         1295 t
ORIGIN      
        1 gcaaacaaat cggcggccgc ccgggaaaac ccgccccgaa cgcgtccgga aaatatgctt
       61 atcgatggaa aacgcagccg catcccccgc cgggcgtttc agacggcaca gccgccgccg
      121 gaaatgtccg acgcttaagg cacagacgca cacaaaaaac cgtatgcctg cacctgcaac
      181 aatccgacag ataccgctgt tttttccaaa ccgtttgcaa gtttcaccca tccgccgcgt
      241 gatgccgcca ccaccattta aaggcaacgc gcgggttaac ggctttgccg tcggcaaagc
      301 agccggatac cgctacgtat cttgaagtat tgaaaatatt acgatgcaaa aagaaaattt
      361 aagtataata cagcaggatt ctttaacgga ttcttaacaa tttttctaac tgaccataaa
      421 ggaaccaaaa tatgaaaaaa gcacttgcca cactgattgc cctcgctatc ccggccgccg
      481 cactggcgga aggcgcatcc ggcttttacg tccaagccga tgccgcacac gcaaaagcct
      541 caagctcttt aggttctgcc aaaggcttca gcccgcgcat ctccgcaggc taccgcatca
      601 acgacctccg cttcgccgtc gattacacgc gctacaaaaa ctataaagcc ccatccaccg
      661 atttcaaact ttacagcatc ggcgcgtccg ccatttatga cttcgacacc caatcgcccg
      721 tcaaaccgta tctcggcgcg cgcttgagcc tcaaccgcgc ctccgtcgac ttgggcggca
      781 gcgacagctt cagccaaacc tccaccggcc tcggcgtatt ggcgggcgta agctatgccg
      841 ttaccccgaa tgtcgatttg gatgccggct accgctacaa ctacatcggc aaagtcaaca
      901 ctgtcaaaaa cgtccgttcc ggcgaactgt ccgccggtgt gcgcgtcaaa ttctgatatg
      961 cgccttattc tgcaaaccgc cgagccttcg gcggttttgt tttctgccac cgcaactaca
     1021 caagccggcg gttttgtacg ataatcccga atgctgcggc ttctgccgcc ctattttttg
     1081 aggaatccga aatgtccaaa accatcatcc acaccgacaa agcccccgcc gccatcggcg
     1141 cgtacagcca agccgtccga gcaggcgaca ccgtttacat gagcggtcaa atccccctcg
     1201 atcccgccac gatgaccgtc gtcggcaacg gcgatttccg cgccgaagcg cgccaagtgt
     1261 tccaaaacct gcaagccgtc gccgaagcgg caggcggcac gctggccgac atcgtcaaac
     1321 tcaacgccta cctgaccgac ttgggcaatt ttgccgtctt caacgaagtg atggcggaat
     1381 ttattgccga gcccttcccc gcccgcgccg ccgtcggcgt tgcctccctg ccaaaaggcg
     1441 tgcaggtcga agccgaagcc gtcctcgttt tgaacgcata attccacgtt cggactgcat
     1501 gatatttcaa aaccatgccg tccgaaccgc aacactttcc cacttcacaa catctgcaat
     1561 ggcacattac gcaatcggcg acatacaagg ctgtttcgac gaactgaccg cgctgctcgg
     1621 caaaatcggt ttcaaccacg gcacggacac cctctggctg acgggcgaca tcgtcaaccg
     1681 cggcccgaaa tccctcgaaa cgctgcaatt ctgcatccgg cacgaaaaca gcgtgcaaat
     1741 cgtcctcggc aaccacgacc tgcacctgct cgctgtcggc tgcggcgaag gcgcgcccaa
     1801 acgcagcgac acaatcgaac ccatactcaa acaccccgac ggaaaaaaaa tgctcgactg
     1861 gctgcgtgcg caaccgctgt tgatacgcga gggcagccgc gtgatggtac acgccggcat
     1921 cctgccgcaa tggcgcatcg ccaaagccga atcgctcgct agagaagccg aagccgaact
     1981 gcgcggcaaa aaatacgtca aattcttctc caaaatgtac ggcaacaaac cggccgcgtg
     2041 ggacgaaggt ttggaaggct atgcccgcct gcgcttcatc gtcaacgcct tcacgcggat
     2101 gcgcgccctg acctttaaaa acgaactgga tttcgactac aaatccacag tgaaaaaaat
     2161 gccgccttac ctgcgcccgt ggttcaaagc ccccgaccgg caaaacctcg accgcaccat
     2221 catcttcgga cactggtcct cgctgggcta cacgaatgcc gacaacgtca tctcgctgga
     2281 caccggcgcg ctgtggggcg gacagctgac cgccgtcaac ctcgaaacgg aagaaattac
     2341 ccaagtccaa gccgccggcg gcatagactg gaaaagcttc gcaaaataac ggaccgcccc
     2401 actatgcaca aaatcctgcc catcgcccac gtcctctccc gactgggtat gctgttttcc
     2461 tttatcctgc tgatacccgc cgccctctcc tacgcctttt cggacggcgc gtacaccgcc
     2521 ttcgccacca ccgcgaccgt taccctttcc ggctcgtgca tcgtccggct cgccaccctc
     2581 cggttcaggc gcgaactgcg cccgcgcgac ggcttcaccc tcgtcctgat gttgtggctg
     2641 gcgtttgccg ctatggcggc gatgccgatg tacctgtatt tcccgaatat gggctttacc
     2701 gacgcatttt ttgaatcgat gtcgggactg accaccaccg gcgcgaccgt catcccccac
     2761 gtcgacgggc tcgccccctc cgtcaacttt tggcggcata tgctcaactg gctgggcggg
     2821 atgggcatca tcgtccttgc cgtcgccatc ctgcctatgc tcggcgtagg cggcacgcag
     2881 ttattcaaag ccgaaatccc cggcattgac aaagaaagca aaatgtcgcc gcgcatttcc
     2941 caagtggcga aaaaactttg gttcggctac accctgatca ccatcctcgc ggcagcctgc
     3001 ctgcattttg ccgggatggg ctggttcgat gccgtctgtc acgcgatggc gaccctctcg
     3061 ctgggcgggt tttccaccca cgatgccagc atagcttatt acaactcccc cctcatcgaa
     3121 gcggtcatca tcgttttcac cattgtcggc ggcatcaatt ttgccaacca tttcgccgcc
     3181 ctcaacagcc gcagcctcaa aacctattgg aaagacgaag aatgccggac gatgctgctg
     3241 ctgctctccg gcagcatcct tgccgccgcc ctgtacctgt ggcacaccgg ctattacgcc
     3301 ggttttaccg aatccctgcg ctacaccgcc ttcaacttcg tctccatcgg actggcaaac
     3361 gggctggcca acaccgactt cgcacaatgg ccgctcctga tttccctgtg gatgtttttc
     3421 ctcgccaaca tcctcgccaa ctccggctcg accggcggcg gcatcaaaac catacgcgcc
     3481 ctcgtcctgt tcaaattcag cctgcgcgaa atgatggtgc tgctgcaccc caaagccgtc
     3541 cgcaccgtca aaatcagcgg caaggccata cccgaccgcc tcgcgctgac cgttatgtcc
     3601 ttcatcttca tctactttat gaccgtcgtc ctcttcagct tcctgctgat ggcgagcggt
     3661 atggaattta ccaccgcctt caccgccgtc atcgcctgca tcaccaacgc cggccccggc
     3721 ctgggcgaag tcgggcccgc cggcaattac gccggtttgg acgtgatgca gaaatggatc
     3781 tgcgttaccg ccatgctctt gggcaggctg gaaatcttca ccgtcttcat cctcttcacc
     3841 cccgcctact ggaagaaata aaaaatgccg tctgaaaact tttcagacgg catattgtct
     3901 tttaaaaaag cggaagctct acaaaaggaa agaaatgctg aaacctctga taaatttcag
     3961 gatatttctc taaggctttt aatgcttttt cttttgaaat aggcggatca tagacatcta
     4021 tcccccttaa gaaggcaatg cccgttaatg catttttttg atcatttgaa atgtaaccat
     4081 aattaacgac tccaattaca tcataaattt cccctatact cttatcaaaa tcaatcaaat
     4141 caaaaatata caatggaatt gtatcaatag gagtatctct gacaactata tccaaccata
     4201 atttaaattc agaaagatta atagcatcat agaacataca gactattgca aatcctaaat
     4261 cttcacaatt ttctttacta attttccaca taattattta cctttatcca ttgcttccca
     4321 accgatacat ccggtatttt agattctcct ttgccgtcaa acgctctttt ggttacctgc
     4381 tttgtatcaa aaatgccgtc tgaaagccga ataccgcttc agacggtatt ttgattgttg
     4441 ggtttttaat cagagctttg tttgataatt tgttattttg aataaaatac aggttctgtc
     4501 gagctgataa cttctattac agaatcgcta aaaaagaaaa tataagcctt ataatatttc
     4561 ttttcaaaaa aatcaaaact ttcttctttc aaccagtcca tataactgtc atctattatc
     4621 tcgaaaaaat aatttatagc aggatttcta tcccaatcat ttttttcttt attttcaaat
     4681 aaatattctg aaaattttac tttataagat tcatctgtta tttgaaaatg aattatattg
     4741 tcaataaata tttcaatatt taattcataa tttcggtcgg atgccaaaat gttcacatct
     4801 tgttcggtaa cacaaatttt gtctacgtaa aatctgcgac tattgttaga aaattgtaat
     4861 ggtttaatct tcactatcgt tttccatatc tgatttttgt tttggtaaca cgacgatcgg
     4921 ggtttcagcg acaccgaggt attttaccgt ctaatcgtgg agattttacc gattttcttt
     4981 cctattccat ccggtcttca aatggttgat gacagtgttt gcttttcagc cgtctgccgt
     5041 tctgaccaag gtgctgccgt ggaaggagca ggtgtaggat ttttgaagaa cttagttccc
     5101 aatatcctgt ttcaaataag gggaaggtcg cctgaaaatt aaacacggtt tcagacggca
     5161 ttttgattgc cggggtttgc tattttttgt tgtaataatc aaatcgtacg ttgactatgt
     5221 ctttctcggt aaaaatataa cggagcatta ctctgaacct ttcataacgc tcattaattt
     5281 tgatactttt aggcaaccaa ggggaagatt taattgta
//
LOCUS       DAXHSF010000139         5314 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.139, whole genome shotgun sequence.
ACCESSION   DAXHSF010000139 DAXHSF010000000
VERSION     DAXHSF010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5314
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.139"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(193..1548)
                     /locus_tag="ACFM8S_001679"
     CDS             complement(193..1548)
                     /locus_tag="ACFM8S_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HFC6314725.1"
                     /translation="MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISTDIDVLNQ
                     NIYNKITTVGKDLVATAKYLSAKYGVPIVNQRISVTPIAQIAAATHADSYVSVAQTLD
                     KAAKAIGVSFIGGFSALVQKGMSPSDEVLIRSIPEAMKTTDIVCSSINIGSTRAGINM
                     DAVKLAGETIKRTAEITPEGFGCAKIVVFCNAVEDNPFMAGAFHGSGEADAVINVGVS
                     GPGVVKAALENSDATTLTEVAEVVKKTAFKITRVGELIGCEASKMLNIPFGILDLSLA
                     PTPAVGDSVARILEEMGLSVCGTHGTTAALALLNDAVKKGGMMASSAVGGLSGAFIPV
                     SEDEGMIAAAEAGVLTLDKLEAMTAVCSVGLDMIAVPGDTPAHTISGIIADEAAIGMI
                     NSKTTAVRIIPVTGKTVGDSVEFGGLLGYAPVMPVKEGSCEVFVNRGGRIPAPVQSMK
                     N"
     gene            complement(1563..1835)
                     /locus_tag="ACFM8S_001680"
     CDS             complement(1563..1835)
                     /locus_tag="ACFM8S_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HFC6314726.1"
                     /translation="MNNSVITVIGKDRVGIVYDVSKILAENRINILNISQQLMDDFFT
                     MIILVDTSKCSKSRQEVLDLFAEESKKLALDIRMQNEEIFQAMHRI"
     gene            complement(1961..2698)
                     /locus_tag="ACFM8S_001681"
     CDS             complement(1961..2698)
                     /locus_tag="ACFM8S_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HFC6314727.1"
                     /translation="MKKLSRIVFSTVLLGFSAALPAQTYSVYFNQNGKLTATMSSAAY
                     IRQYSVAEGIAHAQDFYYPSMKKYSEPYIVASTQIKSFVPTLQNGMLILWHFNGQKKM
                     AGGFSKGKPDGEWVNWYPNGKKSAVMPYKNGLSEGTGYRYYRNGGKESEIQFKQNKAN
                     GVWKQWYADGNIKTEMVMVNDEPAKILTWDESGRLLSELSIHHHKRNGVVLEWYEDGS
                     KKSEAVYQDDKLVRKTQWDKDGYLIEP"
     gene            complement(2979..3878)
                     /gene="prmB"
                     /locus_tag="ACFM8S_001682"
     CDS             complement(2979..3878)
                     /gene="prmB"
                     /locus_tag="ACFM8S_001682"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFC6314728.1"
                     /translation="MFNQAAQELTTIRDILRFAVSRFNEAGLFFGHGTDNAHDEAAYL
                     ILHTLNLPLDMLAPYLDAKLLEAEKEEVLAVIERRAVEHIPAAYLTHQAWQGEFDFYV
                     DERVIIPRSFIYELLGNGLRPWIEYDELVHNALDLCTGSGCLAIQMAHHYPDAQIDAV
                     DVSLDALEVAGINVEDYGLEERIRLIHTDLFEGLEGTYDLIVSNPPYVDAESVELLPE
                     EYLHEPELALGSGADGLDATRQILLNAAKFLNPKGVLLVEIGHNRDVLEAAYPELPFT
                     WLETSGGDGFVFLLTREQLLGEE"
     gene            4023..4616
                     /locus_tag="ACFM8S_001683"
     CDS             4023..4616
                     /locus_tag="ACFM8S_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314729.1"
                     /translation="MVDGMGNLIGWEKTGLVVGKQWITAKDDKVSDVCNANGEMGVIG
                     LYEPFSHGALTIPGHPNCRCEVVSVSGGELGEFAEKKELRKAAMQYARDNFIGKSYVN
                     KNSGHELKVTWQGVKHAASKANQAELSIITKLDDLLRYAKYEGSYSDRKGRPDIIAAH
                     KYRAVTKVGNESLNVGVIVREFPDGHKHYDHFILKDE"
     gene            4728..5314
                     /locus_tag="ACFM8S_001684"
                     /pseudo
     CDS             4728..5314
                     /locus_tag="ACFM8S_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224992.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
BASE COUNT         1463 a         1357 c         1170 g         1324 t
ORIGIN      
        1 agttcaatga gtttattttg tttatactgg cttagacgac tttttctcat agggataatt
       61 ctaacttaat ttgaatttcc ctagttatct aggacagccc catattcttt tctaaagaca
      121 ttttacccga tgcctataaa caaaaatgcc gtctgaagcc tttcagacgg catttcccca
      181 tcaaaaccgc aatcagtttt tcatcgattg aaccggtgcg ggaatcctgc cgccccggtt
      241 gacgaacact tcgcacgaac cttctttgac cggcattaca ggcgcgtagc ccaacaggcc
      301 gccgaactcg acgctgtcgc cgacggtttt acccgttacc ggaataatgc gcacggcagt
      361 ggttttgctg ttaatcatac cgatggcggc ttcgtcggca atgatgccag aaatggtgtg
      421 cgcgggtgtg tcgccgggaa cggcaatcat atccaagccg accgaacaaa cggcggtcat
      481 cgcttcgagt ttatccaacg tcagcacgcc tgcttcggcg gcggcaatca taccttcgtc
      541 ttcggaaacg gggataaacg cgccactcaa accgccaacc gcgctcgaag ccatcatgcc
      601 gccctttttc acggcatcgt tcagcaatgc caaagctgct gttgtgccgt gcgtaccgca
      661 gacgctcaaa cccatttctt caagaatgcg cgccaccgag tcgccgacgg caggggtcgg
      721 tgccagcgac aagtcgagaa taccaaacgg gatattcagc atttttgagg cttcgcagcc
      781 gatgagttcg cccacgcggg taattttgaa ggcggttttc ttcacaactt cggcaacttc
      841 ggtcaatgtc gtcgcatccg aattttccaa cgcggctttg accacgcccg gaccggatac
      901 gccgacatta atcacagcat ccgcttcgcc cgatccgtga aacgcacccg ccataaacgg
      961 attgtcttcc accgcattac agaacacgac gattttcgcg caaccgaaac cttcaggcgt
     1021 gatttcagcc gtgcgtttga tggtttcgcc tgccagcttg accgcatcca tattgatacc
     1081 ggcacgcgtg ctgccgatat tgatggagct gcacacgatg tcggtggtct tcatcgcttc
     1141 gggaatggaa cggattaaca cctcatctga aggcgacatg cctttttgca ccaacgcgga
     1201 aaagccgccg ataaaggaca cgccgatggc tttggcagcc ttatccaaag tttgcgccac
     1261 gctgacatag gaatcggcat gggtagccgc cgcgatttgg gcaatcggcg tgacagaaat
     1321 gcgctgattc acaatcggca cgccgtattt ggcagacaga tattttgccg tcgccaccaa
     1381 gtctttgccg accgtggtga ttttgttgta aatgttttgg tttaacacgt cgatgtcggt
     1441 gctgatgcag tcgtgcaaat caatgccgat ggtaatggtg cggacatcga aattctggtc
     1501 ggcaaccatt ttgacggttt ctaaaatttc gccggattgg atactcatca cattcctccg
     1561 gctcaaatgc ggtgcatcgc ttggaagatt tcttcgtttt gcatacggat gtcgagcgcg
     1621 agttttttgc tctcttccgc aaacaaatcc aaaacctcct gccgcgattt gctgcatttt
     1681 gaagtgtcca ccaagataat catggtaaaa aaatcatcca tcagctgttg gctgatattg
     1741 aggatgttga ttcggttttc cgccaaaatt ttggaaacat cgtacacgat gccgacgcgg
     1801 tctttaccga tgacggtgat gactgaattg ttcacaggct tactccttgc agatatccgt
     1861 taaagtccga aattatacca ccgttggatt ttgaagaaat attgtcaaca atatatacat
     1921 acaaaatgcc gtctgaaact atttcagaca gcatcaagat tcagggttcg attaaataac
     1981 catccttatc ccactgggtt ttcctgacca acttatcatc ctgataaaca gcttcactct
     2041 ttttagaacc atcttcatac cactctaaga ctactccatt acgtttatga tgatggatag
     2101 acagttccga gagtaatcga ccgctttcat cccatgtcag aattttggca ggctcatcat
     2161 tgaccataac catttccgtt ttgatattgc cgtcggcata ccattgcttc catacgccgt
     2221 ttgccttatt ctgtttaaac tggatttcgc tttccttgcc gccgttacgg taatagcggt
     2281 accccgtacc ttcactcaaa ccatttttat aaggcataac ggcagatttt ttaccgttcg
     2341 gataccagtt gacccactcc ccgtccggct tacccttgct gaagcccccc gccatttttt
     2401 tctgaccatt aaaatgccac aaaatcaaca taccgttttg cagggtaggc acaaaagatt
     2461 tgatttgcgt tgaagcaacg atataaggtt cggaatattt cttcatcgac ggataataaa
     2521 aatcctgcgc gtgcgcaata ccctccgcca cactatattg cctgatataa gcggcagaag
     2581 acatcgtcgc cgtcagtttc ccgttctgat taaaataaac agaataggtc tgcgccggca
     2641 aagcggccga aaaacccaac aggacagttg aaaatacaat ccgagataat tttttcattg
     2701 caatagcgat ataaaaacaa ggctgtgttt tagtaatctt ttgatttcaa ttatttgcaa
     2761 gggaaaagac aattattttc cggttaggaa taaacctatt ctattgaata tattgaagcc
     2821 aagtacgcct atcaacacta tattaaaaca ctgccaaaaa caattaactt ataaacaata
     2881 tggtaaggat ttctctgcca agcatcaaac ccgagacaac gtatcgtaaa aatgccgtct
     2941 gaaaacaaat cgtcttcaga cggcatttcc ccttcaactc actcttcacc caataactgc
     3001 tcgcgcgtca agaggaaaac aaaaccgtcg cccccgctgg tttccaacca agtaaaaggc
     3061 aactccggat acgctgcttc caatacatcc ctgttatgcc cgatttccac cagcaataca
     3121 cctttgggat tcagaaactt tgccgcattc agaagaatct gcctggtggc atccaacccg
     3181 tccgccccgc tgcccaatgc caattccggt tcgtgcaaat actcttcagg caataactca
     3241 accgattccg catccacata aggcggattg gaaacaatca aatcataagt gccttccaat
     3301 ccttcaaaca aatccgtatg aataagccgg atgcgttctt ccaaaccata atcttcgaca
     3361 ttaatccctg ccacttccaa agcatccaag ctcacatcaa ccgcatcaat ttgggcatca
     3421 ggataatgat gcgccatctg aatggcaagg caaccgcttc cggtgcaaag atccaaagca
     3481 ttatgcacca gctcatcgta ttctatccaa ggacgaagtc cgttacccaa caattcataa
     3541 ataaaagaac gaggtatgat tacgcgctca tccacataga aatcaaactc tccctgccat
     3601 gcctggtgtg tcaaataagc ggctggaatg tgttcgacag cacgacgctc aataaccgcc
     3661 agcacttcct ctttttcagc ttccaagagt tttgcatcaa gatatggggc aagcatatcc
     3721 aaaggcaaat tcaaagtatg cagaatcaaa taagccgctt catcatgcgc attatctgtt
     3781 ccatgaccaa aaaagagccc tgcctcatta aaacggctga ctgcaaaacg taaaatatcg
     3841 cggatagtcg tcaattcttg tgctgcctga ttaaacataa tatgaaccat tctgcgtata
     3901 gatactttta attataacag aaacaacaag caaacctttt catatcgcca aataaccacc
     3961 caatctaccc atacaactac ataaatgccc gcgcgaaaac catcgcccga acggaaacga
     4021 caatggtcga cggcatgggc aatctgattg gctgggaaaa aacggggctt gttgtcggta
     4081 agcagtggat aaccgcaaaa gacgacaagg tgtccgatgt ctgcaatgcc aacggcgaga
     4141 tgggcgtaat cgggctttac gagcctttct cacacggcgc attgacgata cccggtcatc
     4201 cgaactgccg atgcgaggtt gtttccgtat cgggtggcga attgggagaa tttgccgaaa
     4261 aaaaggagct tcgtaaagcg gctatgcagt atgcgcggga taactttatc ggcaaaagct
     4321 atgtcaataa aaacagcggg catgaactga aggtaacttg gcaaggtgtg aaacacgctg
     4381 cgtcaaaggc aaatcaggcg gaattatcca tcataacaaa acttgatgac ttattgcgct
     4441 acgcaaaata tgagggttct tattcggata ggaaaggtcg tcctgatatt attgcagcac
     4501 ataagtatcg tgccgttacc aaggttggga atgagtcttt aaatgtcggc gtgattgtaa
     4561 gggagtttcc agacggccat aaacattacg accatttcat cttgaaggat gagtaaagcc
     4621 cttttgcagt gtagctctgg agcggatacc gttaaggcaa gtacgcttcc agccttacaa
     4681 aagggcttta aattcagcat accatttata caaacagaaa taaaccaatg acgaagttat
     4741 acgcagaaat cgccaagatg gaggccagga cgacggcacg gtcaaagttt ggggctatgc
     4801 ctcaagcgag gcggtcgatt ccgacggcga agtcatcgcg gcggcagcta tgaaggcggc
     4861 gattcccgat tatatgaagt ttggcgcgga gcgcgagatg cacggctcaa acgctgcggg
     4921 aacggcaatt gaaatcaacg tggaagatga cggcagaacc tttttcgtgg cgcatatcgt
     4981 cgatcccgtt gccgtgacga aggtcaaaac aggcgtttac aagggctttt ccatcggcgg
     5041 cagcgttacc gcccacgatg agttgaacaa gtcgcaaatc acgggtttga agctgacgga
     5101 aatcagcttg gttgaccgac ccgccaatcc cgatgcggtg tttacctgct ttaaggcgga
     5161 caaaggtgcg gaagcggtaa acaacgatac agaacataat gctacatatt ttaaccattt
     5221 cccttccaaa caaaaaagca ccggcggcag ccgatgcccc ttcccttaca ggttccccta
     5281 ttttttagct gcgggcagag ccggtttggc tggg
//
LOCUS       DAXHSF010000140         5308 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.140, whole genome shotgun sequence.
ACCESSION   DAXHSF010000140 DAXHSF010000000
VERSION     DAXHSF010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5308
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.140"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..1343
                     /locus_tag="ACFM8S_001685"
     CDS             <1..1343
                     /locus_tag="ACFM8S_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HFC6314730.1"
                     /translation="TEAGAAIIALAVTVVTSGAGTGAVLGLNGAAAAATDAAFASLAS
                     QASVSFINNKGDVGKTLKELGRSSTVKNLVVAAATAGVADKIGASALNNVSDKQWINN
                     LTVNLANAGSAALINTAVNGGSLKDNLEANILAALVNTAHGEAASKIKQLDQHYITHK
                     IAHAIAGCAAAAANKGKCQDGAIGAVVGEIVGEALVKNTDFSGMTASEIEKAKANITA
                     YAKLVAGATVGVTGGNVDVAANASETAVKNNALDIIWDIGNLVWDGGKWIYAKSIGDK
                     QMAREAAIDFGVDAAAAAVPFVPAGATKISRGGAYVLKAGDEAVDTAKAIQEIQKQTG
                     IKLTYDKVNKVWTTPAGLDYGLDAKHGNRIKHVLAHTIPNPNKPVHSVFNVSRKEVLP
                     LVDEAWRMKGNPLPNDSSVYLVDMKKPIGTKGETKVRIVVQKGTNKIISAYPQK"
     gene            1362..1625
                     /locus_tag="ACFM8S_001686"
     CDS             1362..1625
                     /locus_tag="ACFM8S_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314731.1"
                     /translation="MDKEIKICPRCEQGYLYHAKPKYFSREVILCDECYAMWLGDMKI
                     FYGQYGKDFYDYHEFMKDKGIEEINMWEGELFDHPYYEDEKFK"
     gene            1732..3657
                     /locus_tag="ACFM8S_001687"
                     /pseudo
     CDS             1732..3657
                     /locus_tag="ACFM8S_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238292.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
     gene            3707..4087
                     /locus_tag="ACFM8S_001688"
     CDS             3707..4087
                     /locus_tag="ACFM8S_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314732.1"
                     /translation="MSWVLVILIIGTFYDYYQIRQYAELEKKSISNILLYAQKEKFRL
                     ESKDKYMRGGYTKYEFIFSEYSNTTFLNFINDLKKDNYLPLDGYGHGFLCRKGESISI
                     NIYPEINKFILVWGYPENLCADSN"
     gene            4124..4525
                     /locus_tag="ACFM8S_001689"
     CDS             4124..4525
                     /locus_tag="ACFM8S_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HFC6314733.1"
                     /translation="MRKLNNHDVHKRYQDRLEEDVEFTINYELPLSCLWSTIKDFSSD
                     FEEKTEAFFILFKELLRRGHLKLQRDGQIIGHTPEEWEQIFREVWSEYEIEPNPLPGY
                     APFDIGMWLTVEAPAYAVWIDPEDGSEYWAG"
     gene            4648..5268
                     /locus_tag="ACFM8S_001690"
     CDS             4648..5268
                     /locus_tag="ACFM8S_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314734.1"
                     /translation="MVGVVPGIGESIQAYKVAKAAKNLQGMKKALDKAVTVATAQGYV
                     SKTKIKIGQTELRVTAATDKQLLKAIGEGRDTTGKMTEQLFDSLAKQNGFRVLSGGKY
                     GGNNGFDHVWQAADGSVVLIVESKQIRNGTVQLNPNGAGGYTQMSREWIKQVVKSLPD
                     GSPAKAVVLKANQNGKLKTAIAGVDRQTGKAVILSVKVPSKTNIRR"
BASE COUNT         1677 a         1020 c         1319 g         1292 t
ORIGIN      
        1 taaccgaagc aggtgcggcg attatcgcac tggccgttac cgtggtcacc tcaggcgcag
       61 gaaccggagc cgtattggga ttaaacggtg cggccgccgc cgcaaccgat gcagcatttg
      121 cctctttggc cagccaggct tccgtatcgt tcatcaacaa caaaggcgat gtcggcaaaa
      181 ccctgaaaga gctgggcaga agcagcacgg tgaaaaatct ggtggttgcc gccgctaccg
      241 caggcgtagc cgacaaaatc ggcgcttcgg cactgaacaa tgtcagcgat aagcagtgga
      301 tcaacaacct gaccgtcaac ctagccaatg cgggcagtgc cgcactgatt aataccgctg
      361 tcaacggcgg cagcctgaaa gacaatctgg aagcgaatat ccttgcggct ttggtgaata
      421 ctgcgcatgg agaagcagcc agtaaaatca aacagttgga tcagcactac attacccaca
      481 agattgccca tgccatagcg ggctgtgcgg ctgcggcggc gaataagggc aagtgtcagg
      541 acggcgcgat tggtgcggtc gtgggggaga ttgtcgggga ggctttggtt aagaataccg
      601 atttcagcgg tatgactgct tctgaaattg aaaaagctaa agcgaatatt actgcgtatg
      661 caaaattggt agccggagcg actgtaggtg ttacaggagg caatgttgat gtggcggcaa
      721 atgcttccga aacagctgtt aaaaataatg cattagatat tatttgggat atcggcaacc
      781 tcgtatggga cggcggtaaa tggatttacg ccaaatctat tggcgataag cagatggctc
      841 gagaagcggc gattgatttt ggtgtggatg ccgccgcagc tgccgttccc tttgttccgg
      901 caggtgcgac taaaatcagc cgaggcgggg cttatgttct gaaggcggga gacgaagcag
      961 ttgatacggc taaagccata caggaaattc agaagcagac cggaatcaag cttacttatg
     1021 ataaggttaa taaggtttgg acaacaccgg cggggttaga ttatgggtta gatgctaagc
     1081 atggtaatag gattaaacat gttttagccc atacaattcc aaatccaaac aaacctgttc
     1141 attctgtttt taatgtgtcc cgtaaagaag ttttgccttt ggttgatgaa gcttggagaa
     1201 tgaaaggaaa tcctttgcca aatgattcat ccgtatatct tgtagatatg aagaaaccta
     1261 ttggaacaaa aggagaaaca aaagtgcgga ttgttgtgca aaaaggaaca aataaaatca
     1321 tttctgcata tcctcagaaa taattaagaa aggaatctct tatggataaa gaaattaaaa
     1381 tttgcccaag atgtgagcaa ggctaccttt atcatgcaaa gcctaaatat ttctctaggg
     1441 aggtcatttt atgcgatgaa tgttatgcta tgtggcttgg ggatatgaaa attttttacg
     1501 gacaatatgg aaaagatttt tatgattatc atgagtttat gaaagataaa ggcatagaag
     1561 aaataaatat gtgggaagga gagctttttg atcacccata ttatgaggat gaaaaattta
     1621 aataattgat tttctgttcc ccgaatttgg gaaatacgat gatattttaa acccaaatat
     1681 tatttaaagt agcaataggc cgtctgaata tccgtttttc agacggcctc aatgcaactg
     1741 ctggcagccg aaggcattca ccaacaccaa ttgaatgttc agaaaagtac ccgtttcatc
     1801 ggcatcaaag tgggtaaaag caattacagc aaaaacgagc tgaacgaaac caaactgccc
     1861 gtacgcgtta tcgcccaaac agccaaaacc cgttccggct gggataccgt actcgaaggc
     1921 accgaattca aaaccaccct ttccggagcc gacatacagg caggggtggg tgaaaaagcc
     1981 cgagccgatg cgaaaattat cctaaaaggc atcgttaacc gcatccaaac cgaagaaaag
     2041 ctggaatcca actcgaccgt atggcaaaag caggccggaa gcggcagcac ggttgaaacg
     2101 ctgaagctac cgagctttga agggccggca ctgcctaagc tgaccgctcc cggcggctat
     2161 atcgccgaca tccccaaagg caacctcaaa accgaaatcg aaaagctggc caaacagccc
     2221 gaatatgcct atctgaaaca gcttcagacg gtcaaggacg tgaactggaa ccaagtacag
     2281 ctcgcttacg acaaatggga ctataaacag gaaggcctaa ccggagccgg agccgcaatt
     2341 atcgccctgg cagtagcggc ggtaaccgga ggagtaggcg caggccttat cggagcggca
     2401 cagggtaccg catcggcagc cgtggccgat gctgccttta ccgcattggt gagccaggct
     2461 tccatttccc tgatcaacaa caaaggcaat atcggtaaca ccctgaaaga gctgggcaga
     2521 agccgcacgg tgaaaaatct ggtggttgcc gccgctaccg caggcgtagc cgacaaaatc
     2581 ggcgcttcgg cactgaacaa tatcagcgat aagcagtggg tcaatgacct aaccgtcaac
     2641 ctggccaatg cgggcagtgc cgcgctgatt aataccactg ttaacggcgg cagcctgaaa
     2701 gacaatctgg aagcgaatat ccttgcggct ttggtcaata ccgcgcatgg agaggcagca
     2761 agtaaaatca aacagttgga tcatcattac attacccaca agattgccca tgccatagcg
     2821 ggctgtgctg cggcggcggc gaataagggc aagtgtcagg atggtgcgat cggcgctgcg
     2881 gtcggtgaaa tccttggcga aaccctactg gacggcagag accctggcag cctgaatgtg
     2941 aaggacaggg caaaaatcat tgctaaggcg aagctggcag caggggcggt tgcggcgttg
     3001 agtaaggggg atgtgagtac ggcggcgaat gcggctgctg tggcggtaga gaataattct
     3061 ttaaatgata tacaggatcg tttgttgagt ggaaattatg ctttatgtat gagtgcagga
     3121 ggagcagaaa gcttttgtga gtcttatcga ccactgggct tgccacactt tgtaagtgtt
     3181 tcaggagaaa tgaaattacc taataaattc gggaatcgta tggttaatgg aaaattaatt
     3241 attaacacta gaaatggcaa tgtatatttc tctgtaggta aaatatggag tactgtaaaa
     3301 tcaacaaaat caaatataag tggggtatct gtcggttggg ttttaaatgt ttcccctaat
     3361 gattatttaa aagaagcatc tatgaatgat ttcagaaata gtaatcaaaa taaagcctat
     3421 gcagaaatga tttcccagac tttggtaggt gagagtgttg gtggtagtct ttgtctgaca
     3481 agagcctgct tttcggtaag ttcaacaata tctaaatcta aatctccttt taaagattca
     3541 aaaattattg gggaaatcgg tttgggaagt ggtgttgctg caggagtaga aaaaacaata
     3601 tacataggta acataaaaga tattgataaa tttattagtg caaacataaa aaaataggag
     3661 ttagtatgaa atatatgatt aattttctaa aaaaacattt gaattaatga gttgggtatt
     3721 agtcatacta ataattggga cattttatga ctattatcaa ataaggcaat atgctgaatt
     3781 agaaaagaaa tctatatcaa atatcttgct atatgcccaa aaagaaaaat ttcgcttaga
     3841 gagtaaagat aaatacatgc gaggaggata tacaaaatat gaatttattt tttcagaata
     3901 tagtaatact acttttttaa acttcataaa tgacctgaaa aaagataatt atttaccact
     3961 tgacggctat ggacatggtt ttctatgtag aaaaggagag tctatatcaa tcaatatata
     4021 ccctgaaatt aataaattta ttttagtatg gggataccct gaaaatcttt gcgctgattc
     4081 taattaagta agcaagaata ggttataggg aaataaaatt caaatgagaa aattgaataa
     4141 tcacgatgtt cataaacggt atcaagatcg tttagaagag gatgtagagt tcactatcaa
     4201 ctatgagctt cctttgagtt gtttgtggtc aaccatcaaa gacttttcca gcgattttga
     4261 ggaaaaaact gaagcgttct ttattctttt caaagagctg ctgcgcagag gtcatctgaa
     4321 actgcaacgc gacgggcaaa ttatcgggca tacgcccgaa gaatgggaac aaatatttag
     4381 ggaagtatgg tctgaatatg aaatcgaacc caatccactt cccggctatg ccccgtttga
     4441 tattggaatg tggcttacgg tcgaggctcc tgcctacgcc gtatggatag atcccgaaga
     4501 cggtagcgaa tactgggcgg gataaaatac caatgtttgg aataaatccc gtctgaaaaa
     4561 cagctttttc agacaggatt tattccaaac attggtgata tacagagttt tgtacaagca
     4621 cagaccgctg ccgatcacct gtttgctttg gtgggtgtgg ttccgggtat cggcgaatcg
     4681 atacaggcct ataaagtagc gaaagcggca aaaaatttac aaggcatgaa aaaagccttg
     4741 gacaaggcag taaccgttgc cactgcacag ggctatgtca gcaaaaccaa aatcaaaatc
     4801 ggtcaaactg aattaagggt tactgcagca actgacaaac aattgctgaa agctattggc
     4861 gaaggaaggg acacgacagg taaaatgacc gagcagttat ttgactcttt agctaaacaa
     4921 aatggcttca gagtgctttc gggcggcaaa tacggcggaa ataacggttt tgatcatgta
     4981 tggcaggctg ccgatggtag tgttgttttg attgtagaaa gtaagcagat taggaacggt
     5041 acggtacagc tgaatccgaa tggtgcgggt ggatatacgc agatgagtcg tgaatggatt
     5101 aaacaagttg taaaaagttt acctgatggt agtcctgcta aggcagttgt cttaaaagca
     5161 aatcagaacg gcaaattaaa aacggcaata gcaggcgttg atcgtcaaac aggtaaggcc
     5221 gttattcttt ctgtcaaagt tccttctaaa accaatataa ggagataaca atggggcaca
     5281 atatgatgac cacccaaaaa tggtatga
//
LOCUS       DAXHSF010000141         5269 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.141, whole genome shotgun sequence.
ACCESSION   DAXHSF010000141 DAXHSF010000000
VERSION     DAXHSF010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5269
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.141"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            153..2342
                     /locus_tag="ACFM8S_001691"
     CDS             153..2342
                     /locus_tag="ACFM8S_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC6314735.1"
                     /translation="MCKPNYGGIVLLPLLLASCIGGNFGVQPVVESTPTAYPVTFKSK
                     DVPTPPPAGSSVEITPVNQPAVGAAMRLPRRNIAAFDKNGNEIPDSKQAEEYLPLKEE
                     DILFLDGTPKEQADKLKKEINGRYPNVTIYTSDLKDAVYKYEHVRAGYVYTRFGTDEI
                     EQNSGGKQVTHRLGYDGFVYYSGERPSQSLPSAGTVKYSGNWQYMTDVKRHRAGKAVG
                     IDNLGYITFYGNDVGATSYAAKDVDEREKHPAKYTVDFDKKTLTGQLIKNQYVQKKTD
                     EKKPLTIYNITAKLDGNRFTGSAKVNPDLAKNPAGNERLFFHADADQRLEGGFFGDNG
                     EELAGRFISNDNSVFGVFAGKKTETANAADTKPALPSGKHTKILDSLKISVDEATDGH
                     ARKFAISSMPDFGHPDKLLVEGREIPLVNKEQIIKLADGRKMTVSACCDFLTYVKLGR
                     IKTERPAVKPKAQDEEDSDIDNGEESEDEISEDDNGEDEVTEEEEAEETEEETDEDEE
                     EEPEETEETEEKSPTEGNGGSGSILPALEAPKGRDIDLFLKGIRTAEADIPRTGKAHY
                     TGTWEARIGKPIQWDNQADEKAAKAKFDVDFDRKSISGTLTEQNGVEPAFYIEKGVID
                     GNGFHATAHTRDSGINLSGTGSTSPQSFKADNLLVTGGFYGPKAEELGGTIFNKDGKS
                     LGITENIENEVEAEAEAGVVEQLGPDEVKYQFGVVFGAKKDNKEVEK"
     gene            2339..5173
                     /gene="lbpA"
                     /locus_tag="ACFM8S_001692"
                     /gene_synonym="tbpA"
     CDS             2339..5173
                     /gene="lbpA"
                     /locus_tag="ACFM8S_001692"
                     /gene_synonym="tbpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin-binding protein LbpA"
                     /protein_id="HFC6314736.1"
                     /translation="MNKKHGFQLTLTALAVAAAFPSYAANPETAAPDAAQTQSLKEVT
                     VRAAKVGRRSKEATGLGKIVKTSETLNKEQVLGIRDLTRYDPGVAVVEQGNGASGGYS
                     IRGVDKNRVAVSVDGVAQIQAFTVQGSLSGYGGRGGSGAINEIEYENISTVEIDKGAG
                     SSDHGSGALGGAVAFRTKEAADLISDGKSWGIQAKTAYGSKNRQFMKSLGAGFSKDGW
                     EGLLIRTERQGRETRPHGDIADGVAYGINRLDAFRQTHDIQSNKTGQFFLVEGENTLK
                     PVAKLAGYGIYLNNQLNRWVKERIEKKQPLSAEEEAMVREAQARHENLSAQAYTGGGR
                     ILPDPMDYRSGSWLAKLGYRFGGRHYVGGVFEDTKQRYDIRDMTEKQYYGTDEADKFR
                     DKSGVYDGDDFRDGLYFVPNIEEWKGDKKLVRGIGLKYSRTKFIDEHHRRRRMGLLYR
                     YENEAYSDNWADKAVLSFDKQGVATDNNTLKLNCAVYPAVDKSCRASADKPYSYDSSD
                     RFHYREQHNVLNASFEKSLKNKWTKHHLTLGFGYDASKAISRPEQLSHNAARISEHTG
                     EYTADGKDKYRLGKPEVVEGSVCGYIETLRSRKCVPRKINGSNIHISLNDRFSIGKYF
                     DFSLGGRYDRKNFTTSEELVRSGRYVDRSWNSGIVFKPNRHFSVSYRASSGFRTPSFQ
                     ELFGIDIYHDYPKGWQRPALKSEKAANREIGLQWKGDFGFLEISSFRNRYTDMIAVAD
                     QKTKLPDSSGRLTEIDIRDYYNAQNMSLQGINILGKIDWNGVYGKLPEGLYTTLAYNR
                     IKPKSVSNRPGLSLRSYALDAVQPSRYVLGFGYDQPEGKWGANIMLTYSKGKNPDELA
                     YLAGDQKRYSTKRASSSWSTADVSAYLNLKKRLTLRAAIYNIGNYRYVTWESLRQTAE
                     STANRHGGDSNYGRYAAPGRNFSLALEMKF"
BASE COUNT         1504 a         1234 c         1408 g         1123 t
ORIGIN      
        1 aggttcgtgg gaatgacggg atgtatctgt actgtctgcg gctcgtcgcc ttgtcctgat
       61 ttttgttaat tcactataaa aacggcttga tattatctgt acatattaat ataatgataa
      121 ttattattaa tcaaatagga ggaaaagtag ggatgtgtaa accgaattat ggcggcattg
      181 tcttgttgcc cttacttttg gcatcttgta ttggcggcaa tttcggcgtg cagcctgttg
      241 tcgaatcaac gccgaccgcg taccccgtca ctttcaagtc taaggacgtt cccactccgc
      301 cccctgccgg gtcttcggta gaaatcacgc cggtcaatca gcccgccgtc ggtgcggcaa
      361 tgcggctgcc aaggcggaat atcgcagctt ttgataaaaa tggtaatgaa attcccgata
      421 gtaagcaggc agaggagtat ctgccgctca aagaggagga tatcctgttt ttagacggta
      481 cgccgaaaga acaggctgac aaacttaaaa aggaaatcaa cggacggtat cctaatgtaa
      541 caatctacac gtccgattta aaagatgctg tgtataaata tgaacatgtc cgggccggat
      601 atgtttatac tagatttgga acagatgaaa tcgaacagaa ctcaggtggt aagcaggtta
      661 cccaccgctt aggttatgat ggttttgtat attattctgg agaacgtcct tcccaatctt
      721 taccgagcgc gggaacggtg aaatattccg gaaactggca atatatgacc gatgtcaaac
      781 gtcatcgagc aggtaaggcg gttggcattg acaatttggg ttatatcaca ttttatggta
      841 acgatgttgg tgcaacttct tatgcggcta aggatgtcga cgaaagggaa aaacatcctg
      901 ctaaatatac ggttgatttc gataagaaaa ctttgacagg tcaattaatt aaaaatcagt
      961 atgtgcaaaa gaaaaccgat gaaaagaaac cactgaccat ttacaacatt actgcaaaat
     1021 tggacggcaa ccgctttacc ggcagtgcca aggtcaatcc tgatttagcg aaaaaccctg
     1081 ccggtaatga gcgtttgttt ttccatgccg atgccgatca gcggcttgag ggcggttttt
     1141 tcggcgataa cggagaagag cttgccggac ggtttatcag caacgacaac agcgtattcg
     1201 gcgtattcgc aggcaaaaaa acagagacag caaacgcagc agatacaaaa cctgccctgc
     1261 cgtctggaaa acacaccaaa atcttggatt ctctaaaaat ttccgttgac gaggcgactg
     1321 atggccatgc ccgtaagttt gccatttcct ctatgcccga ttttggtcat cccgacaaac
     1381 ttcttgtcga agggcgtgaa attcctttgg taaacaaaga acaaatcatc aagcttgccg
     1441 acggcaggaa aatgaccgtc agtgcttgtt gcgattttct gacctatgtg aaactcggac
     1501 ggataaaaac cgaacgcccc gccgtcaaac cgaaggcgca ggatgaagag gattcggaca
     1561 ttgataatgg cgaagaaagc gaagacgaaa tttccgaaga tgataacggc gaagatgaag
     1621 tcaccgaaga agaggaagct gaagaaaccg aagaagaaac tgatgaagac gaagaggaag
     1681 aacccgaaga aactgaagaa actgaagaaa aatcgccgac agaaggcaac ggcggttcag
     1741 gcagcatcct gcctgcccta gaagccccta aaggcaggga catcgacctt ttcctgaaag
     1801 gtatccgcac ggcggaagcc gacattccaa gaaccggaaa agcacactat accggcactt
     1861 gggaagcgcg tatcggcaaa cccattcaat gggacaatca ggcggatgaa aaagcggcaa
     1921 aagcaaaatt tgacgttgat ttcgacagga aatcgatttc cggaacgctg acggagcaaa
     1981 acggcgtaga acctgctttc tatattgaaa aaggtgtgat tgatggcaac ggtttccacg
     2041 cgacagcaca cactcgggat agcggcatca atctttcggg aactggttcg accagcccgc
     2101 agagcttcaa agccgacaat cttcttgtaa cgggcggctt ttacggcccg aaggcggagg
     2161 aattgggcgg tactattttc aataaggatg ggaaatctct tggtataact gaaaatattg
     2221 aaaatgaagt tgaagctgaa gctgaagctg gtgttgtcga acagttagga cctgatgaag
     2281 ttaaatacca attcggcgtg gtattcggtg cgaagaaaga taataaagag gtggaaaaat
     2341 gaataagaaa cacggttttc agcttactct gactgccttg gctgttgccg ccgccttccc
     2401 gtcatatgcg gcaaaccctg aaacggcggc acctgatgcg gcgcagaccc aatccctgaa
     2461 agaggttacc gtccgtgccg ccaaagtggg acggcgatcg aaagaggcga ccggtttggg
     2521 caaaatcgtc aaaacgtcgg aaacgttgaa caaagaacag gtactcggta tccgcgacct
     2581 gacgcgctac gatccgggcg tggcggttgt cgaacagggc aacggcgcga gcggcggcta
     2641 ctcgatacgc ggcgtggata aaaaccgtgt ggcggtttcg gttgacggcg ttgcccaaat
     2701 acaggcgttt accgtgcagg gatcgttgag cggatacggc ggacgcggcg gcagcggtgc
     2761 aatcaacgaa atcgaatatg aaaacatcag cacggtggaa atcgataaag gtgccggttc
     2821 gtccgatcac ggcagcggcg cactcggcgg cgcggtcgcc ttccgaacca aagaggcggc
     2881 agacctgatt tcagacggca aaagctgggg gatacaggca aaaaccgcct acggcagtaa
     2941 aaaccgccaa tttatgaagt cgctcggcgc ggggttcagt aaagacggtt gggaagggct
     3001 gctaatccga accgaacgtc aagggcggga aacgcgcccg cacggcgata ttgcagacgg
     3061 ggtggcatac ggcataaacc gtttggatgc gttccgccag acacacgata ttcaaagcaa
     3121 taaaacaggg caatttttct tagtagaggg cgagaataca ctcaagcccg tggcaaaatt
     3181 ggcgggctac gggatatatt tgaacaacca gctcaaccgc tgggtaaaag aacgtattga
     3241 aaaaaaacag cctttaagtg ctgaagaaga agcgatggtg cgggaggcgc aggcgcgcca
     3301 cgaaaacctg tccgcccaag cttacacggg cggcggcagg atattgcccg atccgatgga
     3361 ttaccgcagc ggctcttggc ttgccaagct gggctaccgc ttcggcggca ggcattatgt
     3421 cggcggcgtg tttgaggata ccaaacagcg ttacgacatc cgcgatatga cggaaaaaca
     3481 gtattacggt acggacgagg cggacaagtt tagagacaag agcggggtgt acgacggcga
     3541 cgatttccgc gacggcttgt attttgtgcc gaatatagaa gagtggaagg gcgataaaaa
     3601 gttggtcagg ggcataggtt tgaaatattc ccgcaccaaa tttattgacg aacatcaccg
     3661 ccgccgccgt atgggtttgc tgtaccgtta tgaaaacgaa gcgtattctg acaattgggc
     3721 ggataaggcg gtgttgtcgt ttgacaaaca gggcgtggca accgacaaca acacgctgaa
     3781 gctgaattgc gccgtgtatc ctgctgtgga caaatcctgc cgcgcgtcgg cggacaaacc
     3841 atattcctac gacagcagcg accgtttcca ctaccgcgaa cagcacaatg ttttgaatgc
     3901 ctcgtttgag aaatcgctga aaaacaaatg gacgaaacac catctgactt tgggcttcgg
     3961 ttacgatgct tccaaagcga tctcccgccc cgaacagctt tcccacaatg cggcaaggat
     4021 ttcggaacac acgggggagt acaccgccga cggcaaagac aagtaccgtt tgggtaagcc
     4081 cgaagtcgtc gaaggatcgg tctgcggcta catcgaaacc ctgcgttccc gcaaatgcgt
     4141 gccaagaaaa atcaacggca gcaatatcca tatttctttg aacgaccgtt tttcaatcgg
     4201 caaatatttc gatttcagct tgggcggcag gtatgaccgg aaaaacttca ccacgtcgga
     4261 agaactcgtc cgcagcggac ggtatgttga ccgttcgtgg aacagcggca tcgtgttcaa
     4321 accgaaccgg catttttccg tgtcttaccg cgcctccagc ggcttcagaa cgccctcatt
     4381 ccaagagctt ttcgggatag acatttatca cgattatccg aaaggctggc agcgtcccgc
     4441 cctgaaatcg gaaaaggcag ccaaccgcga aatcggtttg cagtggaagg gcgacttcgg
     4501 ctttttggaa atcagcagct tccgcaaccg ctataccgat atgattgccg ttgccgatca
     4561 aaaaaccaaa ttgccggatt catcaggacg attgacagag attgatatac gcgattatta
     4621 caatgcccaa aatatgtcgc tccaaggcat taatatattg ggaaaaatcg actggaacgg
     4681 cgtgtacgga aaactgcccg aaggtttgta cacgactttg gcgtacaacc gcatcaaacc
     4741 gaaatcggta tccaaccggc cgggactgtc cctccgcagc tatgctttgg atgcggtaca
     4801 gccgtcgcgt tatgttttgg ggttcggata cgaccagccc gaggggaaat ggggcgcaaa
     4861 cattatgctg acctattcca aagggaaaaa ccctgacgag cttgcttatc tggcaggcga
     4921 tcaaaaacga tattcgacaa aaagagcgtc gtcttcttgg tcgacggcag acgtttccgc
     4981 ctatctgaat ctgaaaaaac ggctgacctt gagggcggct atctacaata tcggcaacta
     5041 ccgctacgtt acttgggaat ccttgcgcca gaccgcggaa agcacggcaa accggcacgg
     5101 cggcgacagc aactatggaa ggtatgccgc accgggcagg aacttcagcc tcgcgctcga
     5161 aatgaagttt taaaggaaat accgtctgaa agcttgatct gcaccccaaa agtcggacta
     5221 aaccgccaac tgattaaggt gcaggttttt ttgattcgat ataaacaag
//
LOCUS       DAXHSF010000142         4960 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.142, whole genome shotgun sequence.
ACCESSION   DAXHSF010000142 DAXHSF010000000
VERSION     DAXHSF010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4960
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.142"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            244..4896
                     /gene="iga"
                     /locus_tag="ACFM8S_001693"
     CDS             244..4896
                     /gene="iga"
                     /locus_tag="ACFM8S_001693"
                     /EC_number="3.4.21.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgA-specific serine endopeptidase
                     autotransporter"
                     /protein_id="HFC6314737.1"
                     /translation="MKTKRFKINAISLSIFLAYALTPYSEAALVRDDVDYQIFRDFAE
                     NKGKFFVGATDLSVKNKQGQNIGNALSNVPMIDFSVADVNRRTLTVIDPQYAVSVKHV
                     KGDEISYYGHHNGHLDVSNDENEYRSVAQNDYEPNKNWHHGNQGRLEDYNMARLNKFV
                     TEVAPIAPTSAGGGVETYKDKNRFSEFVRVGAGTQFEYNSRYNMTELSRAYRYAIAGT
                     PYQDVNVTSNLNQEGLIGFGDNSKHHSPEKLKEVLSQNALTNYAVLGDSGSPLFAYDK
                     QEKRWVFLGAYDYWAGYQKNSWQEWNIYKKEFADKIKQRDNAGTIKGYGEHHWKTTGT
                     NSHIGSTAVRLANNERDANNGQNVTFEDNGTLVLDQNINQGAGGLFFKGDYTVKGANN
                     DITWLGAGIDVADGKKVVWQVKNPNGDRLAKIGKGTLEINGTGVNQGQLKVGDGTVIL
                     NQQADADKKVQAFSQVGIVSGRGTLVLNSSNQINPDNLYFGFRGGRLDANGNDLTFEH
                     IRNVDEGARIVNHNTGHASTITLTGKSLITDPKTISIHYIQNNDDDDDGYYYYRPRKP
                     IPQGKDLYFKNYRYYALKSGGSVNAPMPENGQTENNDWVFMGYKQEEAQKNAMNHKNN
                     QRISGFSGFFGEENGKGHNGALNLNFNGKSAQNRFLLTGGTNLNGKISVTQGNVLLSG
                     RPTPHARDFVNKSSARKDARFSKNNEVVFEDDWINRTFKAAEIAVNQSASFSSGRNVS
                     NITANITATDNAKVNLGYKNGDEVCVRSDYTGYVTCNTDNLSDKALNSFDATQINGNV
                     NLNQNAALVLGKAALWGQIQGQGNSSVSLNQHSKWHLTSDSQVHNLSLADSHIHLNNA
                     SDAQSANKYHTLKINHLSGNGHFHYLTHLAKNLGDKVLVKESASGHYQLHVQDKTGEP
                     NQEGLNLFDASSVQDRSRLSVSLANNHVDLGALRYTIKTENGITRLYNPYAENRRRVK
                     PAPSPATNTASQAQTDSAQIAKPQNIVVAPPSPQANQAEEAKRQQAKAEQVKRQQAEA
                     EKVAHQKAEEAKRQQDALARQQAEQERQRLEAERQAAEIAKQKAEAEEAKRRAAEIAE
                     QKAAAEEAKRQAAELARQQEEARKAAELAAKQKAETERKAAEIAEQKAEAEREAAELA
                     KQKAEEEGRQAAQSQPKRRNRRAIPLELSSDATTRALPRIARNSNPDASDYEEIPLDA
                     LEDEDVSESVDTSDKQPQDNTELHEKVETVSLQPRAAQPRAQAAAQPQAQAVAQADAV
                     STNTNSALSDAMASTQSILLDTGASLTRHIAQKSRADAEKNSVWMSNIGYGRDYASAQ
                     YRRFSSKRTQTQIGIDRSLSENMQIGGVLTYSDSQHTFDQASGKNTFVQANLYGKYYL
                     NDAWYVAGDIGAGSLRSRLQTQQKANFNRASIQTGLTLGNTLKINQFEIVPSAGIRYS
                     RLSSADYKLGNDSVKVSSMSVKTLTAGLDFAYRFKVGNLTVKPLLSAAYFANYGKGGV
                     NVGGNSFAYKADNQQKYSAGAALLYRNVTLNVNGSITKGKQLEKQKSGQIKIQIRF"
BASE COUNT         1679 a         1151 c         1068 g         1062 t
ORIGIN      
        1 cacaagcggg aatccaaaaa caaaaagccg cagaaattta acggaagaac cgaaccccga
       61 aaatcggctt cccaccttcg cctgaataac agaacaaagg aagcggaaat aaaaacatcg
      121 ggattctttt tttaaattgg caataaaaca ccaaaatgaa taattcacac aacaaaaaat
      181 agtcaacacc gccaaaccgg cgtaaaatac aaaacattat cacccttaaa acggtaaaac
      241 cttatgaaaa ccaaacgttt taaaattaac gccatatcct tatccatctt tcttgcctat
      301 gcccttacac catactcaga agcggcattg gtcagagacg atgtcgatta tcaaatattc
      361 cgtgactttg cagaaaacaa aggcaaattt tttgtcggcg caaccgattt atcagtgaaa
      421 aacaaacaag gtcaaaacat cggcaacgca ttatccaacg taccgatgat tgattttagc
      481 gttgcagatg tcaacagacg tacattaacc gtcatcgatc cccagtatgc cgtcagcgtc
      541 aaacatgtaa aaggagacga aatctcttat tacgggcatc acaatgggca cttagatgtt
      601 agcaatgatg aaaacgaata tcgttcagtt gcacaaaatg actacgaacc aaataaaaat
      661 tggcatcacg gtaatcaagg tcgtcttgaa gactacaaca tggcacgcct taataaattc
      721 gtaacagaag tcgcacctat tgcaccaacc agtgcaggcg gcggcgttga aacctataaa
      781 gataaaaacc gtttttctga gtttgtgcga gttggagcag gtacgcaatt tgaatataac
      841 agccgctaca atatgacaga gctttcacga gcttatcgtt atgccattgc aggtacgcct
      901 tatcaagacg tgaatgtcac atcgaatcta aatcaagaag gcttgattgg cttcggtgat
      961 aattcaaaac atcattcccc agaaaaactc aaagaagttt tatctcaaaa cgcattgact
     1021 aactatgctg tgttaggcga tagcggttca ccattatttg cttatgataa acaagaaaaa
     1081 cgctgggtct ttttaggtgc ttacgattat tgggcaggct atcaaaaaaa ctcttggcaa
     1141 gaatggaata tctataaaaa agaatttgca gataaaatca aacaacgcga taacgccggc
     1201 accatcaaag gttatggcga acatcattgg aaaaccacgg gcacaaacag ccatatcggt
     1261 tcgacagcgg taaggcttgc caacaatgaa agggatgcaa acaacggaca gaatgttacc
     1321 tttgaagaca acggcacttt ggtattggat caaaacatca accaaggcgc gggcggtctg
     1381 tttttcaaag gcgattacac agtcaaaggt gcaaataatg acatcacttg gctaggtgcg
     1441 gggattgatg ttgccgacgg caaaaaagtc gtttggcaag tcaaaaatcc gaatggcgac
     1501 agattggcaa aaatcggcaa aggcacatta gaaataaacg gcacaggcgt taaccaaggg
     1561 caattaaaag tcggcgacgg tacggttatt ctgaatcaac aagccgatgc cgacaaaaaa
     1621 gtccaggctt tctcccaagt cggcattgtc agcggacgcg gtacattggt attaaatagt
     1681 tcaaatcaga ttaatcccga taacttatat ttcggtttcc gtggcggtcg tttggatgcc
     1741 aatggcaatg acttgacttt tgaacacatc cgcaacgtgg atgaaggcgc gcgcattgtc
     1801 aaccacaaca caggccacgc ctccacaatc acgctaacgg gtaaatcttt gattaccgat
     1861 cccaaaacca tctctattca ttatattcaa aataatgatg atgacgatga tggttattac
     1921 tattaccgcc ctaggaaacc gattccacaa ggcaaagatc tttatttcaa aaactaccgt
     1981 tattacgcct taaaatcagg tggcagtgtt aacgcaccga tgcccgaaaa cggacaaacg
     2041 gaaaataacg actgggtctt tatgggatat aagcaagaag aagcccagaa aaacgcgatg
     2101 aaccacaaaa acaatcagcg tattagcggt tttagcggtt tctttggcga agaaaacgga
     2161 aaaggacata acggcgcatt aaaccttaat ttcaacggca aaagcgcgca aaaccgtttc
     2221 ttgttaacag gcggcacgaa tttaaacgga aaaataagcg taactcaagg caatgtcttg
     2281 ttatcaggtc gtccaacacc acacgcaaga gattttgtga acaaatcttc agcccgaaaa
     2341 gacgcacgtt tctccaaaaa caatgaagtc gtatttgaag acgactggat aaaccgcaca
     2401 ttcaaagccg cagaaattgc ggttaatcaa tccgcatcat tctcttcagg cagaaatgta
     2461 tccaacatta ccgccaacat caccgcaaca gacaacgcca aagtaaattt gggttacaaa
     2521 aacggcgatg aagtttgcgt gcgctcggac tataccggtt acgttacctg caacacagac
     2581 aacttatccg ataaggcttt aaacagcttt gatgcgacac agattaacgg caatgtgaat
     2641 ttgaatcaga atgcggcatt ggtcttaggc aaggctgcat tatgggggca aattcaaggg
     2701 caaggaaaca gcagcgtcag cctaaaccaa catagcaaat ggcatttgac cagcgacagc
     2761 caagtacaca atctgtcatt ggcggatagc catattcatt tgaacaacgc ttccgacgcg
     2821 caaagtgcaa ataaatacca cacgctcaaa atcaatcatt tatccggtaa cgggcatttt
     2881 cattatctga cgcacctggc gaaaaatctt ggggataaag tgcttgtgaa ggaatccgca
     2941 tccggtcatt atcagctcca tgttcaagat aaaacaggcg aacccaatca ggaagggctg
     3001 aatctctttg atgcatcatc cgtacaagac cgctcccgcc tttctgtttc cttggcgaat
     3061 aaccatgtag atttaggggc attgcgttat acaatcaaaa cagaaaacgg tattacccga
     3121 ttgtacaatc cttatgccga gaaccgccgc cgagtcaaac cggccccgtc tcctgccaca
     3181 aacacggctt ctcaagcaca aacggacagt gcacaaattg ccaagcctca aaatatcgtc
     3241 gtcgcaccgc ctagcccgca ggcaaatcaa gccgaagaag caaaacgcca acaagcaaaa
     3301 gcggagcaag tgaagcgtca gcaagcagag gcagaaaaag tcgcacatca aaaggctgaa
     3361 gaagcgaaac gtcagcaaga tgcccttgcc cgccaacaag cagagcaaga acgccaacga
     3421 ttggaagccg aacgccaagc tgccgaaatt gccaaacaaa aagcagaagc cgaagaagcg
     3481 aaacgccgag ccgctgaaat tgccgagcaa aaagcagctg ccgaagaagc aaaacgccaa
     3541 gctgccgaac ttgcacgcca acaagaagaa gcacgaaaag ctgcagagtt ggctgctaag
     3601 caaaaagcgg aaactgaacg caaagcagca gaaattgccg agcaaaaagc ggaagccgaa
     3661 cgtgaagccg ccgaacttgc caagcaaaag gcggaagaag aaggtcgcca agcagcacaa
     3721 tctcaaccca aacgtcgtaa ccgcagggct ataccactgg aactgtcatc tgatgcaaca
     3781 acccgagctt tgcctaggat agcgagaaat tcaaatcctg atgccagcga ttatgaagaa
     3841 atccccttgg atgcgttgga agatgaagat gtatccgaat cggttgacac atcagacaaa
     3901 cagcctcaag acaatacgga acttcatgaa aaagttgaga cagtgagttt gcaaccaaga
     3961 gccgctcagc cgcgagccca agccgccgcg cagccgcaag cccaagccgt cgcgcaagcc
     4021 gatgcagtca gcaccaatac caactcggct ttatctgacg caatggcaag cacgcaatct
     4081 atcttgttgg atacaggtgc ttcattaaca cggcacattg cacaaaaatc acgtgctgat
     4141 gccgaaaaaa acagcgtttg gatgtcgaac atcggttatg gccgtgatta tgcttccgca
     4201 caatatcgcc ggtttagttc gaaacgcacg caaacacaaa tcggcattga ccgcagcttg
     4261 tccgaaaata tgcagatagg cggcgtattg acttactctg acagtcagca tacttttgat
     4321 caggcgagcg gcaaaaatac ttttgtgcaa gccaaccttt atggtaagta ttatttaaat
     4381 gatgcttggt atgtggccgg cgatattggt gcaggcagct tgagaagccg gttacaaacg
     4441 cagcaaaaag caaactttaa ccgagcaagc atccaaaccg gccttacttt gggcaatacg
     4501 ctgaaaatca atcaattcga gattgtcccc agtgcgggga tccgttacag ccgcctgtca
     4561 tctgcagatt acaagttggg taacgacagt gttaaagtaa gttctatgtc agtgaaaaca
     4621 ctgacggccg gactggattt tgcttatcgg tttaaagtcg gcaaccttac cgtaaaaccc
     4681 ttgttatctg cagcttactt tgccaattat ggcaaaggcg gcgtgaatgt gggcggtaat
     4741 tccttcgcct ataaagcaga taatcaacag aaatattcag caggtgccgc gttactgtac
     4801 cgtaatgtta cattaaacgt aaatggcagt attacaaaag gaaaacaatt ggaaaaacaa
     4861 aaatccggac aaattaaaat acagattcgt ttctaaaata ctaaattcat agcaaaataa
     4921 aatgccgtct gaactcaggc ttcagacggc atttttatag
//
LOCUS       DAXHSF010000143         4918 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.143, whole genome shotgun sequence.
ACCESSION   DAXHSF010000143 DAXHSF010000000
VERSION     DAXHSF010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.143"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(267..995)
                     /gene="rmpM"
                     /locus_tag="ACFM8S_001694"
     CDS             complement(267..995)
                     /gene="rmpM"
                     /locus_tag="ACFM8S_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding outer membrane protein
                     RmpM"
                     /protein_id="HFC6314738.1"
                     /translation="MTKQLKLSALFVALLASGTAVAGEASVQGYTVSGQSNEIVRNNY
                     GECWKNAYFDKASQGRVECGDAVAAPEPEPEPEPAPAPVVVVEQAPQYVDETISLSAK
                     TLFGFDKDSLRAEAQDNLKVLAQRLGQTNIQSVRVEGHTDFMGSDKYNQALSERRAYV
                     VANNLVSNGVPVSRISAVGLGESQAQMTQVCEAEVAKLGAKVSKAKKREALIACIEPD
                     RRVDVKIRSIVTRQVVPAHNHHQH"
     gene            1359..2309
                     /locus_tag="ACFM8S_001695"
     CDS             1359..2309
                     /locus_tag="ACFM8S_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HFC6314739.1"
                     /translation="MKLQQLKYALEVYQHNLNVSEAAEALFTSQPGISKQIKLLEEEI
                     GIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHT
                     QARYALPLIVADFVKRYPKVNLTIKQGSPAAIARMVTSGEADLAIVTERIDDHPELGK
                     LPCYDWTHAVIVPNNHPLLECRNPLRIEDLARFPLITYEFAFNAGSSIARAFSKARLE
                     QPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAHLFEPSPTWIAL
                     RSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI"
     gene            complement(2422..3156)
                     /gene="fnr"
                     /locus_tag="ACFM8S_001696"
     CDS             complement(2422..3156)
                     /gene="fnr"
                     /locus_tag="ACFM8S_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFC6314740.1"
                     /translation="MASHNTTHQMKTLCSSCSLRELCLPVGLLPNELSQLDAVIRQSR
                     RLKKGEYLFCVGEAFTSLFAIRSGFFKTTVASQDGRDQVTGFFMSGELIGMDGICSHV
                     HSCDAVALEDSEVCELPFTHIEELGQNIPSLRTHFFRMMSREIVRDQGVMLLLGNMRA
                     EERIAAFLLNLSQRLYSRGFAANDFILRMSREEIGSYLGLKLETVSRTLSKFHQEGLI
                     SVEHKHIKILNLQVLKKMVSGCSHAI"
     gene            3359..4780
                     /gene="hemN"
                     /locus_tag="ACFM8S_001697"
     CDS             3359..4780
                     /gene="hemN"
                     /locus_tag="ACFM8S_001697"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFC6314741.1"
                     /translation="MKIIQIQNNHNVNDDRPEFDRALIASLPASGPRYTSYPTADRFH
                     DGFREGEYIKALHLRGMGALNKPLSLYIHIPFCNTICYYCGCNKIITKDKSRADAYIE
                     YLEKEMELLAPHLNGRHQLAQLHFGGGTPTFLSDEQIERVFRMIRKHFELIPTGEYSI
                     EIDPRKVSRDTVLMLGRLGFNRMSIGIQDFDPKVQAAVNRIQSYEETKEVIDAAREAG
                     FKSVSVDLIYGLPHQTSESIKTTIDTVLSLDPDRLALYHYAHLPHVFKPQRRIDTASV
                     PDSEEKLDMLQYCVQTLTERGYVFIGMDHFAKPDDELSIALKEGFLQRNFQGYSTYAD
                     CDLVAIGVSSIGKIGSTYSQNERDIDAYYAAIDEGRLPIMRGYQLNQDDILRRNIIQD
                     LMCRFALDYRIYESMFGIPFDRYFKDELADLEKLAGLGLVRLNSHGLTVTPKGRFLIR
                     NIAMVFDYHLRHKETKAKYSQTV"
BASE COUNT         1253 a         1339 c         1191 g         1135 t
ORIGIN      
        1 agcacggcaa tgggacacaa acctgaatct gaatctgaat ctgaatctga atctgaatct
       61 gaatctgaat ctaaatctga atccgaatcc gaacctgcac ctcgattctg acgatagccg
      121 tttcagaaaa ccacccaaaa aaacaaaccc cgcaaaagcg ggggtttgtt tcacatcatc
      181 gatgccgacc gacgtttcac gaatattgca acagtacctg gtacaaaatc cactaacctc
      241 atgcatcggc aagatattgc ctagccttag tgttggtgat gattgtgtgc cggcacaacc
      301 tgacgggtta cgatgctacg gattttcaca tccacacggc ggtcaggttc gatacatgca
      361 atcagagcct cacgtttttt ggctttagag actttcgcac ccagtttggc aacttcggct
      421 tcacaaactt gagtcatttg cgcttgagat tcgcccaagc cgacagcaga aattctagaa
      481 acaggtacgc cgttgctgac caggttgttt gccactacgt atgcgcggcg ttcggacagg
      541 gcctgattgt atttgtcaga acccataaag tcggtatggc cttcgacgcg gacagattgg
      601 atattggttt gacccaggcg ttgcgccaat actttcaggt tgtcttgagc ttcggcgcgc
      661 aatgaatcct tatcgaaacc gaacagggtt ttggcagaca gggaaatggt ttcatcaaca
      721 tattgcggag cctgctccac aacgacgaca ggcgcgggtg cgggttcggg ttctggctcg
      781 ggttcggggg cagcaaccgc atcgccgcat tctacgcgac cttggcttgc tttatcaaag
      841 taggcgtttt tccagcattc gccatagttg ttgcgtacga tttcgttcga ctggccgctt
      901 acggtgtaac cctgaacgga cgcctcgccc gcaacagcag tgccggaagc gagcaatgca
      961 acgaataatg cgcttaattt cagctgtttg gtcattttat tccctcatta aatttgtaca
     1021 gcagatacag aagcaactgc tgaattgcca acatacaggc cgctgtagaa acatggcggc
     1081 aaatcggata tttcagtctc cactataaag aagcaggggg aggactgtca atactcaacg
     1141 cctgaaaaca accgtaaaac agcctgtttc ctgatagtct atcatgccga aaaatcaatt
     1201 ccgtgtcatt actttagggg gattttttcc atatcgcacg attgccgttg cacaaaaaca
     1261 acaaacacaa cggcaaagcc ccataccgta ccggcaagaa aatatgataa attataaaca
     1321 atgttcagcc acccgacaca gacccacacc gacccgccat gaaattacaa caattgaaat
     1381 acgccttaga agtttaccag cacaacctga acgtttccga agcggccgaa gccttgttta
     1441 cttcgcaacc cggcatctcc aaacaaatca aattgctgga agaagaaatc ggcattcaga
     1501 tttttatccg cagcggcaag cgcgtggttt cggtctcgca gccgggcaag gtggttttgg
     1561 atattgcgga acgtatttta cgcgatgtac agaacattaa aaatatcggc agcgagttta
     1621 ccggacagga cagcggttcg ctgacggttg ccacgacgca tactcaggca cgctatgccc
     1681 tacccttgat tgttgccgat tttgtgaaac gctatccgaa agtcaacctg accatcaaac
     1741 aggggagccc tgccgccatc gcacgtatgg ttacctcagg cgaggcggac ttggcgattg
     1801 ttacggaacg gatagacgac catcccgaac tgggaaaact tccctgctat gactggactc
     1861 atgcggttat cgtaccgaac aaccacccct tgctcgaatg cagaaacccc ctccgcattg
     1921 aagatttggc gaggtttccg ctgattactt atgaatttgc attcaatgcg ggcagcagca
     1981 tcgcgcgggc attttccaaa gcccgtttgg aacagccgga tgtcgcattg gcggcggcgg
     2041 atacggacgt attgaagact tatgtgcgct tgggtttggg cgtgggactg atggcgaaaa
     2101 tggcgtacaa cccggatacg gacggcgatt tgcagcttgt ggatgcggca cacctgttcg
     2161 agccgtcgcc gacgtggatt gctttgcgca gcgatactta tttgcgcgga tatgcctacg
     2221 actttatcca agcgtttgcg ccgcacctga cacgcgagaa ggtggatagg attctgtaca
     2281 cgcccatcag cgaggatttt tcgatttagg cggctgccgg ttttcagacg gcactttgcg
     2341 gcagatacaa caaacaggac agatgttttc gtctgccctg tgtttattga gaatgccgtc
     2401 tgaaatgttc gtacgggtta atcaaatggc gtgcgaacaa ccggatacca tcttcttcaa
     2461 cacctgcaga ttgaggattt tgatgtgttt gtgttcgacg gaaatcaatc cttcctgatg
     2521 gaacttggat aatgtgcggc tgacggtttc aagtttcagc ccgagataac tgccgatttc
     2581 ttcgcgggac attcttaaga tgaagtcgtt ggcagcaaaa cctcgggaat aaaggcgttg
     2641 ggaaaggttc agcaggaagg cggcaatccg ctcttcggcg cgcatattgc ccaacagcag
     2701 cataacacct tggtcgcgca cgatttcacg gctcatcatg cggaagaagt gcgtacgcag
     2761 gctggggatg ttttgcccca gttcttcgat gtgggtaaac ggcagttcgc acacttcgct
     2821 gtcttccaag gcgaccgcgt cgcaactgtg cacatgggaa cagatgccgt ccatgccgat
     2881 gagttcgccc gacataaaga aacccgttac ctgatcgcgg ccgtcctgac tggcgacggt
     2941 tgttttgaag aagcccgaac ggatggcaaa gagcgaggta aaggcttcgc cgacacagaa
     3001 caggtattcg ccctttttca ggcggcggct ttgacggatg acggcatcga gttggctgag
     3061 ctcgttgggc agcagcccga caggcaggca gagttcccgc aaagaacagg aagaacacag
     3121 cgttttcatc tgatgtgtag tattatgcga agccataccg tacctttttg tgcgctttgc
     3181 cccatcatga ttatagtgga ttaaatttaa accagtacgg cgttgcctcg ccttgtcctt
     3241 atttaaattt aatccactat atgtgcttat tgacacatat caagacaggt ttatcatact
     3301 gtggcattct accaaactat ccaaaatttg actaacatca taaaaccgca cacacaccat
     3361 gaaaatcatt cagatacaga acaatcacaa tgtaaacgat gaccgccccg agtttgaccg
     3421 tgcgctgatt gccagcctgc ccgccagcgg tccgcgctac acttcctacc ctaccgccga
     3481 ccgtttccat gacggtttcc gcgaaggcga atatatcaaa gctttacatt tgcgcggtat
     3541 gggcgcgtta aacaaaccgc tttcccttta cattcacatt ccgttctgca acaccatctg
     3601 ctactactgc ggctgcaaca aaatcatcac caaagacaaa agccgcgccg atgcctacat
     3661 cgaatatctt gaaaaagaaa tggaactgct cgctccacat ctgaacggac ggcaccagct
     3721 tgcccaactg cacttcggcg gcggcacgcc gacctttttg agcgacgaac agatcgaacg
     3781 tgtcttccgc atgatacgca aacatttcga gttaatcccc accggcgaat actccatcga
     3841 aatcgacccg cgcaaagtca gccgcgacac cgtcctcatg ctcggcagac tcggcttcaa
     3901 ccgcatgagc atcggcattc aggatttcga ccccaaagtg caggcggcgg tcaaccgcat
     3961 ccaaagttac gaagaaacca aagaagtcat cgatgcggcg cgcgaagcgg ggttcaaatc
     4021 cgtcagcgtc gatttgattt acggcctgcc gcaccagact tcggaaagca tcaaaaccac
     4081 catcgatacc gttttgtcgc tcgatcccga ccgcctcgcc ctttatcact acgcccacct
     4141 gccgcacgtg ttcaaaccgc aacgccgcat cgataccgcc tccgtccccg acagcgaaga
     4201 gaagctcgat atgctgcaat actgcgtcca aaccctaacc gaacgcggct acgtcttcat
     4261 cggcatggat catttcgcca aacctgacga cgaactctcc atcgccctca aagaaggctt
     4321 cctccagcgc aacttccaag gctattcgac ctacgcggat tgcgatttgg tcgccatcgg
     4381 cgtgtcgtcc atcggcaaaa tcggcagcac ctattcccaa aacgaacgcg acatcgatgc
     4441 ctactatgcc gccatcgacg aaggcagact gcccatcatg cgcggttatc agctcaatca
     4501 ggacgacatc ctgcgccgca atatcattca ggatttgatg tgccgcttcg cgctcgacta
     4561 ccggatttac gaaagtatgt tcggcatccc gttcgaccgc tacttcaaag acgaactggc
     4621 ggatttggaa aaactcgccg gtttgggatt ggtgcgcctg aacagccacg ggctgaccgt
     4681 taccccgaaa ggacgcttcc tcatccgcaa catcgccatg gtgttcgact accacctgcg
     4741 ccacaaggaa accaaggcga aatattcgca aacggtgtga ttgcgtctaa cgtacaaatg
     4801 ccgtctgaaa ggctttttca gacggcattt tgctgccggc aggataagtg ttttcaagaa
     4861 caggcggcgg catatcataa cgttccgcac ctttgtgtcc gaccgttccg aaaccaag
//
LOCUS       DAXHSF010000144         4843 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.144, whole genome shotgun sequence.
ACCESSION   DAXHSF010000144 DAXHSF010000000
VERSION     DAXHSF010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4843
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.144"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(92..574)
                     /locus_tag="ACFM8S_001698"
     CDS             complement(92..574)
                     /locus_tag="ACFM8S_001698"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFC6314742.1"
                     /translation="MTQQRQLPSHELIMSELMMPDTANFSGNVHGGELLLLLDQVAYS
                     CASRYSGNYCVTLSVDKVLFKEPIHIGDLVTFYAAVNYTGRTSMEIGIRVEAQNIRTG
                     EIRHTNSCYFTMVAVKDGKPVPVPPLEILTDRQRCRYEKAKKRRDISLQASEDMSCGC
                     "
     gene            complement(727..888)
                     /locus_tag="ACFM8S_001699"
     CDS             complement(727..888)
                     /locus_tag="ACFM8S_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314743.1"
                     /translation="MSGQPEKHHTSPIEDERKNPVYRMGQAVAGFMLVVWVSVLALVF
                     FLVFRFWLS"
     gene            complement(962..1423)
                     /gene="recX"
                     /locus_tag="ACFM8S_001700"
     CDS             complement(962..1423)
                     /gene="recX"
                     /locus_tag="ACFM8S_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFC6314744.1"
                     /translation="MKPQKSLRARAMDILSRQEVSRIGLKRKLAPYAESEEELENVLN
                     EFAERNWQSDLRYAEAYIRSKSRKHGSLRLKQALAQQGIDEETSRNLLPDRSSEKQAA
                     IAVLRKKFKHPAADLKEKQKQARFLAYRGFDADTVQTALKHAWDENWEDGC"
     gene            complement(1496..2212)
                     /locus_tag="ACFM8S_001701"
     CDS             complement(1496..2212)
                     /locus_tag="ACFM8S_001701"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFC6314745.1"
                     /translation="MNAAIEHVQAVAFDLDGTLCDSVPDLAAAAEAMLEQLGMKPLPA
                     KVVESYVGDGIGKLVHRVLTNDRDRDREADSELWEKGFVSYMKYYRDHLSVFTRPYPE
                     TEAGLALLKSLGIPLAVITNKNEILAVELLKQLGLADYFSLILGGDSLPEKKPSPLPL
                     RHAAEVLGIDVANMVMVGDSRNDIIAAKAVGCLSVGVTFGYGDMTLLSQDDATRPDWI
                     IGSLPEIYENLQPQKNKDEE"
     gene            2619..3884
                     /locus_tag="ACFM8S_001702"
     CDS             2619..3884
                     /locus_tag="ACFM8S_001702"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HFC6314746.1"
                     /translation="MSEALAKETLNPFEIARKQVKTACDRLKTDPAVYEILKSPTRVL
                     EVNFPVKLDDDTVKTFTGYRSQHNNAVGPYKGGVRFHPGVNLDEVKALSIWMTIKCCV
                     AGIPYGGGKGGITLDPRDYSEAELERIARAYSEAISPLIGEKIDIPAPDVNTNGKIMS
                     WMVDAYENVVKHSAPGVFTGKPVEFGGSLARTEATGYGVNLAAVQALEKLGKDVKGAT
                     YAIQGFGNVGYHTGYYAHQSGAKVVAVSTVDVAIYNENGLDMEALFKEFQEKGFITNE
                     AGYGKEITNAELLALDVDVLAPCALENQLTSENAGKVRAKIVVEGANGPTTPEADVIL
                     RQNGVLVVPDILANCGGVVVSYFEWVQNLQGYYWEFDEVQEKETVVLRRAFRDIWNLA
                     QEYDVDLRTASYMMSIRRVEKAMKLRGWY"
     gene            complement(3942..4748)
                     /locus_tag="ACFM8S_001703"
     CDS             complement(3942..4748)
                     /locus_tag="ACFM8S_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HFC6314747.1"
                     /translation="MKKTLTLLAVSALFAASAHAHRVWVETAHTHGGEYLKADLGYGE
                     FPELEPIAKDRLHIFSKPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLVIAEYQ
                     PTFWSKNKAGWKQAGIKEMPDASYCEQTRMFGKNIINVGHESADTAIITKPVGQNLEI
                     VPLDNPANIHVGERFKVRVLFRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDST
                     DDKGEVDIIPLRQGFWKANVEHKADFPDQSVCQKQANYSTLTFQIGHSHH"
BASE COUNT         1049 a         1099 c         1420 g         1275 t
ORIGIN      
        1 aatttaatcc actatatttg cttgatttca tccgtttgca atggaaacgg atggcgcgat
       61 gtgcctgtct ttcagacggc atagtccgcc gtcagcagcc gcaggacatg tcttcagagg
      121 cttgcaggct gatgtctctg cgttttttgg ctttttcgta gcggcagcgt tggcggtcgg
      181 tcaggatttc cagcggaggg acagggacgg gtttgccgtc tttgaccgca accatcgtga
      241 agtagcagct gttggtgtgg cggatttcgc cggtgcggat gttttgtgct tcgacacgga
      301 tgccgatttc catcgaggtg cgtcccgtgt agttgacagc ggcgtaaaag gtaaccaaat
      361 cgccgatgtg gatgggttct ttaaacagga ctttgtcaac cgacagggta acgcaataat
      421 tgccgctgta acggctggcg caggaatagg cgacttggtc gagcaggagc aggagttcgc
      481 cgccgtgtac gttgccgctg aaattggcgg tgtccggcat catcagttcg gacataatga
      541 gttcgtggga aggcagttgg cgttgttgtg tcatagtgta cctgttttaa gaaagggtgt
      601 tttgaaatgg gtgtgggaaa attttaggga atggggaagt ggaattcaag ctaaaaaaag
      661 ctatgatggg atagtctgcc gaaatggacc gccgatgcca tctgaagcct tcagacggca
      721 ttttgtttag gaaagccaaa aacggaagac caggaaaaac acgagtgcca atacagagac
      781 ccaaacgacg agcatgaatc cggcaactgc ctgccccatg cggtaaaccg ggtttttgcg
      841 ttcgtcttcg atgggggagg tgtggtgttt ttcgggttgt ccggacattt tgttctcaat
      901 cggaaaagtt ggaaacgggc gtaaggttat gttgccgtgc aaaaagattc aagaaggaag
      961 gtcagcagcc gtcttcccaa ttttcgtccc aagcgtgttt caatgccgtc tgaacggtat
     1021 cggcatcgaa tccgcgatag gcgaggaaac gcgcctgttt ttgtttttct ttgaggtcgg
     1081 cggcggggtg tttgaatttt ttacgcaaca cggctatggc ggcctgtttt tcgcttgagc
     1141 ggtcgggaag caggttgcgg ctggtttctt catctatgcc ctgttgcgcc aaagcctgtt
     1201 tcagcctcaa tgaaccgtgt ttgcggcttt tgctgcggat ataggcttcg gcatagcgca
     1261 aatccgactg ccagttgcgt tcggcaaatt cgtttaacac gttttccaac tcctcttcgc
     1321 tttcggcgta cggtgcaagt ttgcgtttca gaccgatgcg gctgacttct tggcgcgaga
     1381 ggatgtccat cgcgcgggcg cgtagggatt tttgcggttt catggcggcg gtttcggcac
     1441 ggaaagattc agacggcatg ggaaacggat tccgatgccg tctgaacaga ttgttttatt
     1501 cttcgtcttt atttttctgc ggttgcaggt tttcgtaaat ttcgggcagc gagccgataa
     1561 tccagtcggg gcgggtcgca tcgtcttgcg agagcagcgt catatcgccg taaccgaagg
     1621 taacgccgac gctcaggcag ccgacggctt tggcggcgat gatgtcgttg cgcgagtcgc
     1681 cgaccataac catgtttgca acatcgatac ccaaaacttc ggcggcatgc cgcagcggca
     1741 gggggctggg ttttttctcg ggcaggctgt cgccgccgag tatcaggctg aagtagtcgg
     1801 caagtccgag ctgtttcaac agttcgacgg caaggatttc gtttttgttg gtaatgactg
     1861 ccagcgggat gcccaaagat ttgagcaatg ccagcccggc ttcggtttcg ggataggggc
     1921 gggtgaagac gctcaaatgg tcgcggtagt atttcatata ggatacgaaa cctttttccc
     1981 acagttcgga atcggcttcg cggtcgcggt cgcggtcgtt ggtgaggacg cggtgaacca
     2041 gtttgccgat gccgtcgccc acatagcttt cgaccacttt ggcgggcagc ggtttcatac
     2101 cgagttgttc caacatcgct tctgcggcgg cggcaaggtc ggggacggaa tcgcacagtg
     2161 tgccgtccaa atcgaaggcg acggcttgga cgtgttcgat ggctgcattc ataatctgat
     2221 ttcctgtcgg gttgaaaggg tttattttaa catcgtttgc acagggcttc taatgtatag
     2281 attgttaaca atcgcaacag gttttgcagg cggtgttttc ggatttcggg gcggaatcgg
     2341 gcaagggttt gaaatcccag cgattttcca attaaatttt taaaaaataa aaggatgcct
     2401 atatctttat ttaatttgac atttattttt cggacgcgcg cgctgctttt tcaggcgaag
     2461 tccgggctga ttccgtgcgg cttgaaaaat aaaatcatat atagattaat caaatcgggc
     2521 ttatgccgac tacacgctat tacattttgt cgggaaattg gttatagtaa tgagcgaaat
     2581 gcacgcccct gaaaatcttt ttgtgaaaag gaagcaaaat gtctgaagcc cttgcaaaag
     2641 aaaccctcaa tccgtttgaa atcgcgcgca aacaggtcaa aaccgcctgc gaccggctaa
     2701 aaaccgatcc ggccgtttat gaaatcctga agagtccgac ccgcgtgttg gaagtcaatt
     2761 ttcccgtcaa actcgacgac gacacggtca aaaccttcac gggctaccgt tcgcaacaca
     2821 acaacgccgt cggcccctat aaaggcggcg tgcgcttcca tcccggagtg aatctggacg
     2881 aagtcaaagc cttgtcaatt tggatgacca tcaaatgctg cgttgcgggt attccttatg
     2941 gcggcggcaa aggcggcatt accttggatc cgcgcgatta ttccgaggcg gaactggaac
     3001 gcatcgcccg cgcttattcc gaagccattt ctccgctgat cggcgagaaa atcgatattc
     3061 ccgcacctga tgtgaacacc aacggcaaaa tcatgtcttg gatggttgat gcctatgaaa
     3121 acgtggtgaa acattctgca ccgggcgtat ttaccggtaa accggttgag ttcggcggct
     3181 ctttggcgcg taccgaagcc acgggttacg gcgtgaacct cgccgccgtc caagccttgg
     3241 aaaaactggg caaagacgtg aagggtgcca cttacgccat tcaaggtttc ggtaatgtgg
     3301 gttaccacac aggttattac gcgcatcaat ccggcgcgaa agttgttgcg gtttctactg
     3361 tcgatgttgc catttacaac gaaaacggtt tggatatgga agccctgttc aaagagttcc
     3421 aagaaaaagg cttcatcacc aacgaagccg gttacggcaa ggaaattacc aacgccgaac
     3481 ttttggcttt ggatgtggac gtactcgccc cttgcgcgct ggaaaaccaa ctgacttccg
     3541 aaaacgccgg taaagtccgc gcgaaaatcg tggtcgaagg cgcgaacggt ccgactacgc
     3601 ccgaagccga cgttatcctg cgtcaaaacg gcgtattggt cgtgcccgat attctggcga
     3661 actgcggcgg cgtggtcgtt tcctatttcg agtgggtgca aaacctgcaa ggctattatt
     3721 gggagtttga cgaagttcag gaaaaagaaa ccgtcgtcct gcgccgtgcg ttccgcgata
     3781 tatggaactt ggcgcaagag tatgatgtcg atttgcgaac cgcgtcttac atgatgagta
     3841 tccgccgtgt tgaaaaagcg atgaaactgc gcggttggta ttgatgtttg aacaaaaaat
     3901 gccgtctgaa gccttcagac ggcatttttg tgcgggcggg attaatggtg cgaatggccg
     3961 atttggaagg ttaaagtcga gtagttcgcc tgtttttggc acacgctttg atcggggaag
     4021 tcggctttgt gttcgacatt ggctttccag aagccttggc gcaaggggat gatgtccact
     4081 tcgcctttgt cgtctgtgct gtcggagaaa gcctgtgctt cggttttgtg cgttttgctg
     4141 cggtcgctgg tgtcgaagcc gtcaaaggtg gcggtaacgg tggcattggg cagcggttcg
     4201 ccacggaaca gaacgcggac tttgaagcgt tcgcctacgt gaatgttggc gggattgtcc
     4261 agcgggacga tttccaagtt ttgtccgacc ggtttggtga tgatggcggt gtccgcgctt
     4321 tcgtgtccga cgttgatgat gtttttgccg aacatacggg tttgttcgca atagcttgcg
     4381 tcaggcattt ctttgatgcc cgcctgtttc cagcctgctt tgttttttga ccagaaagta
     4441 ggctgatatt cggcgatgac gaggtaactg ccgtccttaa cgggacggtt gcttcggtac
     4501 tggtagttgt atgtgccgcg ttgaatcatg ttttccttgc ctttttcggt aaccagctgc
     4561 atcggtttgc tgaagatgtg caggcggtct ttggcgatgg gttcgagttc gggaaattcg
     4621 ccgtagccca agtcggcttt aaggtattcg ccgccgtgcg tgtgggcggt ttcgacccag
     4681 acgcggtggg cgtgggcgga tgcggcaaat agggcggaaa cggcgagcag tgtcaatgtt
     4741 ttcttcatgg tgcagccttt gtctcgtgtg ggttaaagat tttgttatgt tataacaaaa
     4801 taggtatcgt gtgaagacgg atttgctgtc tgaaaactgt ctt
//
LOCUS       DAXHSF010000145         4772 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.145, whole genome shotgun sequence.
ACCESSION   DAXHSF010000145 DAXHSF010000000
VERSION     DAXHSF010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.145"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(<1..485)
                     /locus_tag="ACFM8S_001704"
     CDS             complement(<1..485)
                     /locus_tag="ACFM8S_001704"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC6314748.1"
                     /translation="MKIILTTSMSGLGGTETATVRLGRLLKRRGHDIILASSDGPFVG
                     EAQASGIRWQPVDFYRGGLAGYLKSTFAYARMLRREQPDIIDCQMARVVPACALAAKI
                     VSPKTKIIYHSHGLDAATYPKIAKLFDKLGVYIIGNCKHEREKLIRHGFPAGRIAYAY
                     NA"
     gene            complement(482..1903)
                     /locus_tag="ACFM8S_001705"
     CDS             complement(482..1903)
                     /locus_tag="ACFM8S_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFC6314749.1"
                     /translation="MDTKEILGYAAGSIGSAVLAVIILPLLSWYFPADDIGRIVLMQT
                     AAGLTVSVLCLGLDQAYVREYYAAADKDTLFKTLFLPPLLSAAAIAALLLSRPSLPSE
                     ILFSLDDAAAGIGLVLFELSFLPIRFLLLVLRMEGRALAFSSAQLVPKLAILLLLPLT
                     VGLLHFPANTAVLTAVYALANLAAAAFLLFQNRCRLKAVRHAPFSPAVLHRGLRYGIP
                     IALSSIAYWGLASADRLFLKKYAGLEQLGVYSMGISFGGAALLFQSIFSTVWTPYIFR
                     AIEENAPPARLSATAESAAALLASALCLTGIFSPLASLLLPENYAAVRFIVVSCMLPP
                     LFCTLVEISGIGLNVVRKTRPIALATLGALAANLLLLGLAVPSGGARGAAVACATSFW
                     LFFAFKTESSCRLWQPLKRLPLYTHTLLCLTSSAAYTCFGTPANYPLFAGVWAVYLAG
                     CILRHWKDLHKLFHYLKKQGFPL"
     gene            complement(2098..3207)
                     /gene="ribD"
                     /locus_tag="ACFM8S_001706"
     CDS             complement(2098..3207)
                     /gene="ribD"
                     /locus_tag="ACFM8S_001706"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFC6314750.1"
                     /translation="MFSDTDISMMETALRLAALGRFSTSPNPRVGCVIAHGSQIVGQG
                     FHVKAGEPHAEVHALRQAGEMAQGATAFVTLEPCSHYGRTPPCAEALLRSGVTRVVAA
                     MRDPNPLVAGKGLALLEAAGIKTECGLLEHQARELNRGFLSRIERRRPFVRLKCAVSL
                     DGKTALSDGSSFWITGEDARADVQVLRAESCAVLTGIGTVLADNPRLNVRAFPTLRQP
                     ARIVLDSRLRLPLDSHLLTDGQSPTYIATLERDEDKLRPYREHAHIRILMPSETAGGK
                     IDLNHLMRLLADEGFGEIMVEAGSELTSAFLAEDLADEIVLYRSPKILGSGKDLLSLP
                     ENRAALSAPPLWTPVSSEILGHDIKTVFRKNDNAF"
     gene            complement(3240..3704)
                     /locus_tag="ACFM8S_001707"
     CDS             complement(3240..3704)
                     /locus_tag="ACFM8S_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFC6314751.1"
                     /translation="MKCPFCAHPDTRVADSRLMEERNAVRRRRHCPSCGKRFGTLETA
                     ELKMPAVIGPDKKRSPFNAQRLRNDLTAAARKSALTPEQIDETVRLTKHRLYTSGQRD
                     IPSAALADMVLKELLEHNTEAAVRFAALHKRFDNPADFASWLAQAVKTDRKA"
     gene            complement(3733..4575)
                     /locus_tag="ACFM8S_001708"
     CDS             complement(3733..4575)
                     /locus_tag="ACFM8S_001708"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFC6314752.1"
                     /translation="MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGK
                     GVRLFHDDKPSANSPVADLVRAYQIKSENVVAHIRKASQGQTSLANTHPFMREMWGGY
                     WLFAHNGHLIDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDAIAG
                     LTHEIRKFGLFNFMLSDGIALFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTP
                     SDRVAVIATLPLTRDESWSQLAVDELVMFREGNIVRHDRPENPVYMSAEEGLEIARAA
                     GVSV"
BASE COUNT          954 a         1224 c         1627 g          967 t
ORIGIN      
        1 gcgttgtagg cgtaggcaat ccggccggcg gggaagccgt ggcggatgag tttttcgcgc
       61 tcgtgtttac agttgccgat gatgtacacg cccagcttgt cgaagagctt ggcgattttg
      121 gggtaggttg ccgcgtccaa accgtgcgag tggtagatga ttttggtttt cggcgaaacg
      181 attttggcgg caagggcgca ggctggaaca acgcgcgcca tttggcagtc gatgatgtcg
      241 ggttgttcgc ggcgcagcat ccgggcgtag gcaaaggtgc ttttgaggta gccggcaagc
      301 ccgccccggt aaaaatcaac cggctgccaa cggatgcccg atgcttgcgc ctctccgaca
      361 aaaggcccgt ccgaagaagc aaggatgatg tcgtgcccgc gccgtttgag cagccgcccg
      421 aggcggacgg tggcggtttc cgtgccgccc aagcccgaca tagaagtggt caggatgatt
      481 ttcataatgg aaacccttgt tttttcaaat aatgaaacag tttgtgcaaa tctttccagt
      541 ggcgcaggat gcagcctgcc agatataccg cccatacgcc ggcaaacagg gggtagtttg
      601 ccggtgtgcc gaagcaggtg taggccgccg aggaggtcag gcagagcaat gtgtgcgtat
      661 aaagcggcag gcgtttgagc ggctgccaca ggcggcagga gctttcggtc ttgaaggcaa
      721 aaaacaacca gaatgaggtg gcacaggcaa ccgccgcgcc gcgcgcgccg ccggacggta
      781 cggcaagccc cagcagcagc aggtttgccg ccagcgcgcc caaggtggcg agcgcgatcg
      841 ggcgtgtttt tcggacgacg ttcaaaccga tgccgctgat ttctaccagc gtgcaaaaca
      901 gcggcggcag catacacgat acgacgataa accggacggc agcgtagttt tccggcagca
      961 ggagggaggc gaggggcgag aaaatgccgg tcaggcagag ggcggaggca agcagggcgg
     1021 cggcggattc tgccgttgcc gagaggcggg cgggcggggc gttttcttcg attgcgcgga
     1081 aaatatacgg tgtccagacc gttgaaaaga tgctttggaa caataatgcc gccccgccga
     1141 acgaaatacc catcgaataa acgccgagct gttccagacc ggcatatttt ttcaggaaca
     1201 aacggtcggc ggatgccagc ccccaatagg cgatgctgct cagtgcgatc ggtatgccgt
     1261 agcgcagccc ccggtgcagg acggcgggcg aaaacggtgc gtgccggacg gccttcagac
     1321 ggcatcggtt ttgaaacagc aaaaaggcgg cggcggcaag gtttgccagc gcgtaaacgg
     1381 cggtcaggac ggcggtgttc gccggaaagt gcagcagccc gaccgtcagc ggcagcagca
     1441 gcaggatggc gagcttgggc acgagttgcg cggacgaaaa ggcaagggcg cgcccttcca
     1501 tacgcaaaac cagtaagaga aagcggatgg gcaggaagct cagttcaaac agcaccagcc
     1561 cgatgccggc ggcggcatcg tcgagcgaaa acaggatttc agacggcagg gacgggcggg
     1621 aaagcagcag ggcggctatc gcggcggcag acagcagcgg cggcaggaac agggttttga
     1681 acaaggtgtc tttgtcggcg gcggcatagt attcgcggac gtatgcctga tccagcccga
     1741 ggcacaatac cgacaccgtc agtcccgccg ccgtctgcat cagcacgatg cgcccgatgt
     1801 cgtcggcggg gaaataccac gacagcagcg gcaggatgat gacggctaaa accgcgctgc
     1861 cgatcgagcc tgccgcgtag ccgaggattt cttttgtgtc catttttgat gtccgggcgg
     1921 cggcgggatg ctgcctgtgc cgtctgaagc ctttcttgat cggaatttga cggctttcag
     1981 tccgttgcgg ctgccggcgg ggtgcggcag tccgggttgc gcttttccgg gcgggcggcg
     2041 gtctgaacgg ggccgttttt tatcggcgtt attatatagt gaaacggcgc aaacccttta
     2101 aaaggcgtta tcgtttttcc ggaacacggt tttgatgtcg tgtccgagga tttcgcttga
     2161 aacgggtgtc cacaagggcg gtgcggaaag ggcggcgcgg ttttcgggca gggaaagcag
     2221 gtctttgccg ctgccgagga ttttgggcga acggtacagc acgatttcgt ccgccagatc
     2281 ttctgccaga aatgcggatg tgagttcgga gcctgcttcg accatgattt ctccgaaacc
     2341 ttcgtcggca aggaggcgca tcaggtggtt caggtcgatt ttgccgcctg ccgtttcaga
     2401 cggcatcagg atgcggatgt gtgcgtgttc ccgatagggg cggagtttat cttcatcgcg
     2461 ttccaaggtg gcgatgtagg ttggagattg tccgtcggtc agaaggtggc tgtccaaggg
     2521 caggcgcagg cggctgtcta acacgatgcg tgcgggttgg cgcaaagttg gaaaagcgcg
     2581 gacgttgagc cggggattgt ccgccaacac cgtgccgatg ccggtcagca ccgcgcagct
     2641 ttcggcacgc aaaacctgta cgtcggcacg cgcgtcttcg ccggtaatcc aaaagctgct
     2701 gccgtctgaa agggcggttt tgccgtccag cgaaacggcg catttgaggc ggacgaaggg
     2761 gcggcggcgt tcgatgcgcg acaggaagcc tcggttgagt tcccttgcct gatgttcgag
     2821 taaaccgcat tccgtcttga tgcctgctgc ttcgagcagg gcaagccctt tgcctgcaac
     2881 cagcgggttg gggtcgcgca tggcggcgac gacgcgtgtt acaccggaac ggagcagagc
     2941 ttcggcgcag ggcggtgtgc gtccgtaatg gctgcacggt tcgagggtaa caaaggcggt
     3001 cgcgccttgt gccatttcgc ccgcctgacg cagggcgtgg acttcggcgt gcggttcgcc
     3061 cgctttgacg tggaagcctt gcccgacaat ttggctgccg tgtgcgataa cgcagccgac
     3121 gcgcggattg ggcgaagtgg aaaaacgtcc caaagcggca agtcggaggg cggtttccat
     3181 catggatata tctgtgtccg aaaacatagg gataccgtat cagtatgggt tggggagaat
     3241 caggctttgc ggtctgtttt gacggcttgc gccagccacg aggcaaagtc tgccggattg
     3301 tcgaagcgtt tgtgcagggc ggcgaaacgg acggcggctt ccgtgttgtg ctcaagcagc
     3361 tcttttagca ccatgtcggc aagtgcggca gaggggatgt cgcgctgacc cgaagtgtag
     3421 agcctgtgtt tcgtcaggcg gacggtttcg tcgatctgtt cgggtgtcag ggcggatttt
     3481 cgggcggcgg cggtcaggtc gttgcggagg cgttgtgcat taaagggcga acgttttttg
     3541 tccggaccga tgacggcggg cattttgagt tcggcggttt cgagcgtgcc gaagcgtttg
     3601 ccgcagctgg ggcagtggcg gcggcggcgc acggcgttgc gttcttccat caggcgcgaa
     3661 tcggcgacgc gggtgtcggg gtgggcgcaa aacgggcatt tcatagcgtt tcttcctgat
     3721 gccgtctgaa cgtcaaaccg atacgccggc ggcgcgggcg atttccagac cttcttcggc
     3781 actcatatag acggggtttt cgggacggtc gtgtcggacg atgttgcctt cgcggaacat
     3841 gaccagttcg tccacggcaa gttgggacca tgattcgtcg cgggtcagtg gcagggtggc
     3901 gataacggcg acgcggtcgg agggcgtggt tacttcggca aaatcgacca tcacgtcgtc
     3961 gtcgagcagg cgcgccttgc cgaacggggc ttggcggacg atgtagtgca gcagtgtgct
     4021 ggcgtgggca aacagggcaa tgccgtctga aagcataaag ttaaacagcc cgaacttgcg
     4081 gatttcgtgc gtcagccccg caatcgcgtc aaacagcgtg tcgtcgtcgg gacgggcggc
     4141 aaagcgggtg cgcaggcggt tgaggatgtg gcagaacgcg cgttcggaat cggttgtgcc
     4201 gacggggtgg aaaaattcgc cctgttcggg gaaaaaatca atcaaatgtc cgttgtgggc
     4261 aaacagccag tagccgcccc acatttcgcg cataaaggga tgggtgttcg ccagcgaggt
     4321 ttgtccttgt gatgctttgc ggatatgggc gacgacgttt tccgatttaa tctggtaggc
     4381 gcgcacgagg tcggcgacgg gcgaattggc actgggtttg tcgtcatgga acaggcgcac
     4441 gcctttgcct tcgaaaaagc cgatgccgaa accgtcggca tggtggtcgg taatgccgcc
     4501 cctgcggcgg aagccttcaa aggaaaacat aatatcggtc ggcgtattgc agttcatgcc
     4561 cagcagttga cacatggttt gtcccaatga ttcagatggt cgcaagtatt cggattatac
     4621 cccgaactga aaatgccgtc tgaaatacgg cttgttcccc attattccct cgaaaacaga
     4681 aaacaaagac ggaaacttaa gattccgtca ttcccgcgca ggcgggaatc cgacttgtcc
     4741 ggttttggtt gtttttcgtt ccgtaacttt tg
//
LOCUS       DAXHSF010000146         4727 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.146, whole genome shotgun sequence.
ACCESSION   DAXHSF010000146 DAXHSF010000000
VERSION     DAXHSF010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4727
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.146"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            165..944
                     /gene="pgeF"
                     /locus_tag="ACFM8S_001709"
     CDS             165..944
                     /gene="pgeF"
                     /locus_tag="ACFM8S_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HFC6314753.1"
                     /translation="MKTITETLNLAPKGKNFLTADWPAPANVKTLITTRNGGVSQGAY
                     QSLNLGTHVDDNPEAVRRNREIVQQQVGLPVAYLNQIHSTVVVNAAEALGGTPDADAS
                     VDDTGKVACAVMTADCLPVLFCDRAGTTVAAAHAGWRGLAGGVLQNTIAAMKVPPVEM
                     MAYLGPAISADAFEVGQDVFDAFCTPMPEAAAAFEGIGGGKFLADLYALARLILKREG
                     VGGVYGGTHCTVLERDTFFSYRRDGATGRMASLIWLDGNAV"
     gene            996..1475
                     /gene="lptE"
                     /locus_tag="ACFM8S_001710"
     CDS             996..1475
                     /gene="lptE"
                     /locus_tag="ACFM8S_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFC6314754.1"
                     /translation="MNKLFLTAAVLMLGACGFHLKGADGISPPLTYRSWHIEGGQALQ
                     FPLETALYQASGRVDDAAGAQMTLRIDSVSQNKETYTVTRAAVINEYLLILTVEAQVL
                     KRGEPVGKPMTVSVRRVLAYADNEILGKQEEEAALWAEMRQDAAEQIVRRLTFLKAE"
     gene            1477..2475
                     /gene="holA"
                     /locus_tag="ACFM8S_001711"
     CDS             1477..2475
                     /gene="holA"
                     /locus_tag="ACFM8S_001711"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFC6314755.1"
                     /translation="MAAHIGRIDTDAPLKPLYVIHGEEELLRIEALDALRAAAKKQGY
                     LNREVYTADNAFDWNELLQTAGSAGLFADLKLLELHIPNGKPSKTGGEALQDFAARLP
                     EDTVTLVLLPKLEKTQLQSKWFAALAAKGEVWEAKPVGAAALPQWIRGRLDKIGLGIG
                     ADALALFAERVEGNLLAARQEIDKLALLYPKGHTVNIDEAQTAVANVARFDAFQLAGA
                     WMKGDVLRVCRLLDGLREEGEEPVLLLWAVAEDVRTLIRLAAALKQGQSIQSVRNSLR
                     LWGDKQTLAPLAVKRISVVRLLDALKTCAQIDRIIKGAEEGDAWTVFKRLVVSLAE"
     gene            2596..3015
                     /locus_tag="ACFM8S_001712"
     CDS             2596..3015
                     /locus_tag="ACFM8S_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314756.1"
                     /translation="MDNKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAIIVAA
                     AAGGFVWTSRRQQRQFIERLKKFDIDPEKGRINEANLRRMYHSGGQHQKDAITLICLS
                     QKCSVDEAHAMFKKRPTRQEINQMAAKQSRGQKRPHR"
     gene            3091..3606
                     /locus_tag="ACFM8S_001713"
     CDS             3091..3606
                     /locus_tag="ACFM8S_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFC6314757.1"
                     /translation="MMILKTAPLKRRFAAMLYEMLLVGAATCLAALFAGIAAIFLNPV
                     SIAVSALVTSILIMGSWWLYFRANWHGQGQTLAMRTWKIGLCDLNGIQPSLYLLRLRF
                     IWACIFIVFIPMLAYAGLRHFLGIPPKGAAGAALIWLILPWGFALLNPDRQFLYDFLA
                     GTRLVAVKGKP"
     gene            complement(3690..4574)
                     /locus_tag="ACFM8S_001714"
     CDS             complement(3690..4574)
                     /locus_tag="ACFM8S_001714"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFC6314758.1"
                     /translation="MSSGNIHIHSMTGFANAAAECGSKRINLDLRAVNHRFLDIQIRM
                     PDDLRYLESGIREKISSHIARGKVECKIQIQDAENGSQSLELNRDLVGQLAEINKDLR
                     KHHDLAKLGVADILRFPGVLASQRENTEELAKSITELTEKALKDFTAARKREGKKLGE
                     HLLQRLEAMEEIIDALSELFPTLLETHKEKIRARLVEAVGSIDNDRLQQEFALFIQKS
                     DIDEEFSRLRTHIAEVRRIVTEHKGSSGKRLDFLMQELNREANTLGSKSIATECTQAS
                     VELKVLIEQMREQVQNIE"
BASE COUNT         1053 a         1254 c         1324 g         1096 t
ORIGIN      
        1 taagggcatc attcccgcac cggcaagaat ccaaacccga acgtttgaaa acaatcccga
       61 atctccgaat tcccgcctgt gcgggaatga cgaaaaaaca aacatccatt tgccccgaag
      121 gcagttaatc aaccctttcc gccacacacc tattccaata tccaatgaaa accatcacag
      181 aaaccctaaa tctcgccccg aaaggcaaaa acttcctgac cgccgattgg cccgcgcccg
      241 ccaatgtgaa aaccctgatt accacgcgca acggcggcgt gagccaaggt gcgtatcaga
      301 gtttgaacct cggtacgcac gtcgacgaca atcccgaagc cgtgcgccgc aaccgcgaaa
      361 tcgtgcaaca gcaggtcgga ttgcccgttg cctacctcaa tcaaatccac agtaccgtcg
      421 tcgtcaatgc tgccgaagcg ttgggaggca cacccgatgc ggacgcttcc gtagacgaca
      481 cgggcaaagt tgcctgtgcc gtgatgaccg cagactgcct gcctgttttg ttttgcgaca
      541 gggcgggtac gacggttgcc gccgcacacg cgggctggcg cggtttggcg ggcggcgtat
      601 tgcaaaacac cattgccgcg atgaaggttc cgcctgtcga aatgatggcg tatctcggcc
      661 ccgccatcag tgcggatgcg tttgaagtcg gacaggacgt gtttgatgcg ttctgcacgc
      721 ccatgcccga agccgccgcc gcatttgaag gcataggtgg cggcaaattc cttgccgacc
      781 tttacgcgct cgcccgcctg attctgaagc gcgaaggcgt gggcggcgta tatggcggca
      841 cgcattgtac ggttttggaa cgggatactt tcttttccta ccgccgcgac ggagcgacag
      901 ggcgtatggc gagcctgatt tggctggacg gcaatgccgt ctgaacacgc cgctgatata
      961 atctaccgac tttgtgcttt tgagaaaggc aagccatgaa caaactgttt cttactgccg
     1021 cagtgctgat gctgggcgcg tgcggtttcc acctgaaagg tgcagacggc atttctccgc
     1081 cgctgaccta ccggagctgg cacatcgaag gcggacaggc attgcagttt cctttggaaa
     1141 ccgcgctgta tcaggcttcg ggcagggtgg acgatgctgc cggcgcgcag atgaccctgc
     1201 gtatagacag cgtttcccaa aacaaggaaa cctacaccgt tacccgtgcg gcagttatca
     1261 acgaatatct tttaatcttg acggttgaag cgcaggtatt gaaacgcggc gagccggtcg
     1321 gcaaaccgat gaccgtgtcc gtccgccgcg tccttgctta tgccgacaac gagatcttgg
     1381 gcaaacagga agaggaagcg gcattgtggg cggaaatgcg gcaggatgcc gccgaacaga
     1441 ttgtccgccg cctgaccttt ctgaaggcgg aatgacgtgg cggcacatat cggacgcatt
     1501 gatacggacg cgcctttgaa acccctgtac gtcatccacg gcgaggaaga actgttgcgt
     1561 atcgaggcat tggacgcatt gagggcggcg gcgaagaaac aaggttacct taatcgggaa
     1621 gtttatacgg cagacaatgc tttcgattgg aacgagctgc tgcaaaccgc aggcagtgcg
     1681 ggtctgtttg ccgatttgaa gctgttggaa ctgcatatcc ctaacggcaa gcccagcaaa
     1741 accggcggcg aggcgttgca ggattttgcc gcccgcctgc cggaagatac ggtaacgctg
     1801 gttttactgc ccaaactgga gaaaacccag ctccagtcca aatggtttgc cgcattggcg
     1861 gcaaaggggg aagtgtggga agccaaaccg gtcggcgcgg cggctttgcc ccaatggata
     1921 cgcggacggc tggacaaaat cggtttgggt atcggggcag acgcattggc actgtttgcc
     1981 gagcgcgtgg aaggcaatct gttggcggca cgtcaggaaa tcgacaagct cgcgctgctg
     2041 tatccgaaag ggcataccgt caatatcgat gaggcgcaaa ccgccgttgc caacgtcgcc
     2101 cgcttcgacg cgttccaact ggcaggcgcg tggatgaagg gcgatgtcct gcgcgtatgc
     2161 aggcttttgg acggattgcg ggaagagggc gaagaaccgg tgctgttgct gtgggcggtt
     2221 gccgaagacg tgcggacgct gatccggctt gctgccgccc tgaagcaggg gcagagcatc
     2281 caatccgtcc gcaacagcct taggctttgg ggcgacaagc agacgctcgc accgcttgca
     2341 gtcaagcgga tttccgtcgt ccgcctgctt gacgcgctca aaacctgtgc ccaaatcgac
     2401 cgaatcatca aaggcgcgga agagggcgac gcatggacgg tattcaaacg gcttgtcgtg
     2461 tcgctggcgg aataaagcgg taatccccaa aatccgaaaa tactgtaaaa taccgttaat
     2521 cctgaaaagt attcaccaat ccgtccgaaa acatttcaga cggcacgacc acctcaataa
     2581 aggaacatta accctatgga caataagacc aaactgcgct tgggcggcct gattttactg
     2641 accaccgccg ttttaagcct cattatcgta ttgattgtcg attcctggcc gcttgccatc
     2701 ctgcttgccg ccatcatcgt cgccgccgct gcgggcggtt ttgtttggac atcccgccga
     2761 cagcaacgcc agtttatcga acgcctgaaa aaattcgaca tcgatcccga aaaaggcaga
     2821 atcaacgagg caaacctgcg ccgtatgtac cacagcggcg gacaacacca gaaagatgcg
     2881 attaccctga tctgcctgtc gcaaaaatgt tcggtggacg aggcgcacgc tatgttcaaa
     2941 aaacgcccga cacgtcagga aatcaatcaa atggcggcaa aacagtcgcg cggtcagaaa
     3001 cgtccgcacc gttaaccgcc gcaaggcatc tttgcataaa tgccgtctga agcctgttgg
     3061 cgtttcagac ggcatattct gattgaaaag atgatgatac tgaaaaccgc cccgctcaaa
     3121 cgccgctttg ccgccatgct gtatgagatg ctgctggtcg gtgcggcaac ctgtttggca
     3181 gcattgtttg ccggtattgc cgccattttt ctgaatcccg tttctatcgc ggtttctgca
     3241 ttggtaacga gtatcctgat aatgggatca tggtggcttt attttcgcgc caactggcac
     3301 ggtcaggggc agaccttggc gatgaggaca tggaaaatcg gcttgtgcga ccttaacggc
     3361 atacagccgt ctttgtacct gttgcgcctg cgctttattt gggcgtgcat attcatcgtc
     3421 ttcattccta tgcttgctta tgccggattg cgccacttcc tcggcattcc gcccaagggc
     3481 gcggccggcg cggcattgat ttggctgatt ttaccgtggg ggttcgcact gctgaatccc
     3541 gaccggcagt ttctgtatga ttttcttgca ggaacaagat tggtggcggt caaaggaaag
     3601 ccttaagcct ttataccgca aaggtttcaa actgaaaaaa tgccgtctga aagggctttc
     3661 agacggcatt tgcttatcgg ggaaaccgat tattcgatat tctgcacctg ttcgcgcatc
     3721 tgttcgatca ggactttcag ctcgaccgag gcttgggtgc attcggtagc gatggatttg
     3781 ctgcccaaag tgttggcttc gcggtttaat tcctgcatca ggaagtccag ccgtttgccg
     3841 ctgctgcctt tgtgttcggt aacgatacgg cgcacttcgg caatgtgggt gcgtaggcgg
     3901 ctgaactctt cgtcgatgtc ggatttttgg ataaagaggg caaattcctg ttgcaggcgg
     3961 tcgttgtcga tgctgccgac cgcttcgacg aggcgggcgc ggattttttc tttatgtgtt
     4021 tccaacaggg taggaaagag ttcgcttaat gcatctatga tttcttccat agcctcaagg
     4081 cgttgcagca ggtgctcgcc taatttctta ccttcccgct tgcgtgcggc agtaaagtct
     4141 tttaacgctt tttcggtcag ttcggtaatg ctttttgcca attcttccgt attttccctt
     4201 tggcttgcca atacgccggg gaaacgcagg atgtcggcaa cgcccagttt tgccaaatcg
     4261 tgatgcttgc ggaggtcttt gttgatttcg gcaagctgtc cgaccaagtc gcgattcagt
     4321 tccaaggact gactgccgtt ttccgcatct tgaatttgga ttttgcattc gactttgccg
     4381 cgtgcgatat gggatgaaat tttctcgcgg ataccgcttt ccaaatagcg caaatcgtcg
     4441 ggcatcctga tttgaatatc tagaaaacgg tggttgaccg cacgcaggtc gagattgatg
     4501 cgtttgctgc cgcactctgc cgccgcgttg gcaaatccgg tcatgctgtg gatgtggata
     4561 tttccgctgc tcatgtcgtt ctccgaagcc cgttaaaatg gaatcaatat atcacatctg
     4621 tatggcggca agcgttttcg ggtgtgaaaa attgaagatt ttgcagcggc agattggaat
     4681 cacgcgcttt tgttgctgca aggaagggaa atgtatagtg gattaac
//
LOCUS       DAXHSF010000147         4704 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.147, whole genome shotgun sequence.
ACCESSION   DAXHSF010000147 DAXHSF010000000
VERSION     DAXHSF010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4704
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.147"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(417..953)
                     /locus_tag="ACFM8S_001715"
     CDS             complement(417..953)
                     /locus_tag="ACFM8S_001715"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFC6314759.1"
                     /translation="MDLREVKLGGETIYEGGFVSISRDKVRLPNGNEGQRIVIRHPGA
                     ACVLAVTDDEKVVLVRQWRYAANQATLELPAGKLDVAGEDMAACALRELAEETPYVAD
                     SVRLLYSFYTAVGFCNEKMYLFEAEGVRLGSTLANDEDEITETVLMSKEEVRQALAND
                     EIKDGKTLIGLQYWLMKD"
     gene            complement(987..1343)
                     /gene="folB"
                     /locus_tag="ACFM8S_001716"
     CDS             complement(987..1343)
                     /gene="folB"
                     /locus_tag="ACFM8S_001716"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HFC6314760.1"
                     /translation="MDKIFLHGIKADTLIGVYGWERERLQTLIVDLDIGVPEKAGSDD
                     DIANTVHYAEVCETLRRHLKEQDFLLLEALAEYIADLVLGYFGAVWVRVKIVKPGILE
                     GVREVGVEIERGKRED"
     gene            1399..2001
                     /gene="plsY"
                     /locus_tag="ACFM8S_001717"
     CDS             1399..2001
                     /gene="plsY"
                     /locus_tag="ACFM8S_001717"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFC6314761.1"
                     /translation="MFNIPAVAVSYLIGSLSFAVIVSKYYGMDDPRTYGSGNPGATNV
                     LRSGKKKAAALTLLGDAAKGLVAVLLARVLQEPLGLSDSAIAAVALAALVGHMWPVFF
                     GFKGGKGVATALGVLLALSPATALVCALVWLVMAFGFKVSSLAALTATIAAPLAALFF
                     MPHTSWIFATLAIAILVLLRHKSNIVKLLEGRESKIGGSR"
     gene            2078..2923
                     /locus_tag="ACFM8S_001718"
     CDS             2078..2923
                     /locus_tag="ACFM8S_001718"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HFC6314762.1"
                     /translation="MLSYRHAFHAGNHADMLKHFTLFLVLQYFNRKDKPYWYIDTHGG
                     AGVYNLEGSEAQKVGEYRQGIALLRQAKNLPTELNGFIAHIQKILPSPELYCGSPWLA
                     QSLTRVGDKLRLFELHPTDFAHLQNNMGEAGLGKRGQVLREDGYKGLISLLPPPPRRA
                     TVLIDPPYEEKQDYRRVTETLKAALKRFESGCYLIWYPCLSREESRKLPEELKKLLPD
                     NYLHAELHVHAPKADGFGMHGSGMFVINPPYLLAEQLAANLPALTRLLAQDEGARYLL
                     DSKIR"
     gene            3169..4143
                     /locus_tag="ACFM8S_001719"
     CDS             3169..4143
                     /locus_tag="ACFM8S_001719"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFC6314763.1"
                     /translation="MDTLTRFLPEHLQQNQLPEALGGVLLSVVSACTEINAKVRLGAL
                     AGVLGMAGTGNIQGEDQKKLDVIANNIMIDTLKANPAVAGLASEEEDSFVSAGENGRY
                     LVLFDPLDGSSNIDVNISVGTIFSILEKPEGALATESFLQTGRQQLAAGYILYGPQTQ
                     LVFTFGHGVYMFTLNAENEFVLTKEKPKVPESTKEFAINMSNRRHWLPPVQQYIDELL
                     AGETGTRGKNYNMRWVASMVAEIHRILMRGGVFMYPQDKRDPSKPGKLRLMYEANPMS
                     LILEQAGASASNARQDILGIRPESLHQRVAVIMGSSEEVDYLNRLHSK"
     gene            complement(4302..4520)
                     /locus_tag="ACFM8S_001720"
     CDS             complement(4302..4520)
                     /locus_tag="ACFM8S_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFC6314764.1"
                     /translation="MFPEYRDLISKLKQENSRFARLFDEHNELDDKITGLVNNPVTSG
                     AETIDELKKAKLKLKDELYAILQKAAGK"
     gene            complement(4496..>4704)
                     /locus_tag="ACFM8S_001721"
     CDS             complement(4496..>4704)
                     /locus_tag="ACFM8S_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314765.1"
                     /translation="GKAENLCVKRDIVNVFINRTAVLKENFAVSDKTLDKNIIFVIIN
                     VKIQFDFLTVNQERKAICSQNTVI"
BASE COUNT         1211 a         1396 c          994 g         1103 t
ORIGIN      
        1 tctcaaattc aaattgggct tttaggtcta taacaaagaa aaagccttgg taggataaac
       61 ctaccaaggc tttttcttaa aaaaacgctc tattaccgtg cctaaaaatg caaaaaagta
      121 accgtcataa cccttttgcc aaaatcaata tcatcatccg atgaatgaat tgacggtata
      181 taagaaagaa ttaataagct cataaaccgg taagaaaaat cagtaacaaa gatattcatt
      241 attaaccatt ttaaaaatta tcatgttcgt ttgggtaatt attataccct tttccctctt
      301 attactaaag cacgaatgac tttaaaggac gcgcccagag acaagccgaa aaggcatatc
      361 tcatccgcca aacaaaatgc ccaccgactt actccggcag gcagttcaac accctgtcaa
      421 tccttcatca gccagtattg caaaccgatt aatgtcttgc cgtctttaat ttcatcgttt
      481 gctaatgcct gacggacttc ttctttcgac atcaatacgg tttccgtaat ctcgtcttcg
      541 tcattggcaa gcgtactgcc caaacgcacg ccttccgctt cgaacagata cattttttcg
      601 ttgcaaaaac cgaccgccgt ataaaaactg taaagcaggc gcacgctgtc ggcaacataa
      661 ggcgtttctt cagccagttc tctcagcgca cacgctgcca tatcctcgcc cgccacatcc
      721 agcttgcccg caggaagttc caatgtcgcc tgatttgccg cataccgcca ctgccgtacc
      781 aaaacgacct tttcatcatc cgttaccgcc aacacgcacg ccgctcccgg atgacggatg
      841 acgatacgct gcccttcatt gccgttgggc aacctgacct tatccctgct gatgctgaca
      901 aaaccgccct cgtaaatggt ttcgccgcct aattttactt ccctcaaatc cacttcatat
      961 ctcctttccg tttcctattc tgccgttcaa tcttcacgct tgccgcgctc gatttccacg
     1021 ccaacctcgc gcacgccttc caaaataccc ggcttgacga ttttcacgcg tacccacacc
     1081 gcgccgaaat atcccaaaac caaatcggca atatattccg ccaacgcttc caaaagcagg
     1141 aaatcctgtt ctttcagatg tcggcgcagt gtttcgcata cctcggcata atgtaccgta
     1201 ttggcaatat cgtcgtccga acccgctttc tcgggaacgc cgatgtccaa atcgacaatc
     1261 agggtctgca accgttcgcg ttcccagccg tacacgccga taagcgtatc cgccttgatg
     1321 ccgtgcaaaa agattttatc cataatccgc cccgtttttt gctaaaataa ccgcaccatt
     1381 ctaagtaaag taccacaaat gttcaacata ccggctgttg ccgtttccta tctgatcggc
     1441 tcactttctt tcgccgtcat cgtttccaag tattacggga tggacgaccc gcgcacctac
     1501 ggatcgggca atcctggggc gaccaatgtt ttacgcagcg gcaaaaaaaa ggcggccgcg
     1561 ctgacgctct tgggcgatgc cgccaaaggt ttagttgccg ttttgcttgc acgcgtgctt
     1621 caagaaccgc tcggtttatc cgacagcgca atcgctgccg tcgcactcgc cgcgctggtc
     1681 gggcatatgt ggccggtgtt tttcggattt aagggcggca aaggcgtggc aacggcattg
     1741 ggcgtgcttc tggcactctc tcccgcaact gccttggtct gcgcgttggt ttggcttgtt
     1801 atggcattcg gcttcaaggt gtcctccctt gccgcattaa ccgccacaat cgccgccccc
     1861 cttgccgcac tgttttttat gccgcatact tcttggattt tcgcaaccct cgcaatcgcc
     1921 atattggtgt tgctccgcca caaaagtaat atcgtcaagc tgctcgaagg cagagaaagc
     1981 aaaatcggcg gcagccgctg atttcccatc catccctgtt tatgccgtct gaaccccgct
     2041 tcagacggca tccgaatatc ccgccgtttt accgactatg ctcagttaca gacacgcatt
     2101 ccacgccggc aaccacgccg atatgctcaa acacttcacc ttgtttttag tgctgcaata
     2161 tttcaaccgc aaagacaagc cctactggta catcgacacg cacggcggcg cgggtgtgta
     2221 caatctcgaa ggcagcgagg cacaaaaagt cggcgaatac cggcaaggca tcgccctact
     2281 tcgacaagca aaaaacctgc caaccgagtt aaacggcttt atcgcgcaca tacaaaaaat
     2341 cctgccctcg cccgaacttt actgcggttc tccgtggctg gcacaatcgc tgacccgcgt
     2401 cggcgacaaa ttacgcctgt ttgagctgca ccccaccgac tttgcccatc tgcaaaacaa
     2461 tatgggcgaa gcaggattgg gcaaacgcgg acaagtgttg cgcgaagacg gttacaaagg
     2521 gctgatttcc ctgctgccgc ccccgccgcg ccgcgccacc gtcctcatcg acccgcccta
     2581 tgaggaaaaa caggactacc ggcgtgtaac ggaaacgctg aaagccgctt taaaacggtt
     2641 tgagtccggc tgttatctca tttggtatcc ctgcctcagc cgcgaagaaa gccgcaagct
     2701 gcccgaagaa ttgaaaaaac tcctgcccga caactacctt cacgccgaac ttcacgtcca
     2761 cgcgccgaaa gccgacggtt tcggtatgca cggcagcgga atgttcgtca tcaatccgcc
     2821 ctatctgttg gcggaacaat tggcggcaaa cctccccgcc ctgacgcggc tgctggcgca
     2881 agacgaaggc gcgcgctacc ttttggacag caaaatccgc tgaccccgat gccgtctgaa
     2941 acccttcaga caacatctcc cgtcaaataa acaaaaccgg acgatatacc ggcagtcggc
     3001 acttttaaga ataaatatcc cctcatccgc ctgccgcgtt ccgacttgcc ccatgccgtt
     3061 tttttacact acaatcccct agactatcct gcaaaacgca ccaaaggaat acgccacaat
     3121 aacctgccgc ccttcagacg gcacaaacct ttccgaaacg agcaaactat ggacacactg
     3181 acccgattcc tccccgaaca cctgcaacaa aaccagctgc ccgaagcact cggcggcgtt
     3241 ttactgtccg ttgtttccgc ctgcaccgaa atcaacgcca aagtccgcct cggcgcactg
     3301 gccggcgtat tgggtatggc gggcacgggc aatattcagg gcgaagacca aaaaaaactg
     3361 gatgttatcg ccaacaacat catgattgac acactcaaag ccaaccctgc cgttgccggt
     3421 ttggcaagcg aggaagaaga ctctttcgta agtgccggtg aaaacggacg ctatctcgtc
     3481 ctattcgacc ctttagacgg atcgtccaat attgatgtca acatttccgt cggtacaatt
     3541 ttctccatcc ttgaaaaacc cgaaggcgca ttggcaaccg aatcattcct tcaaacgggc
     3601 agacagcagc ttgccgcagg ctatatcctc tacgggcctc aaacccagct cgtattcaca
     3661 ttcggacacg gcgtatatat gttcacgctc aatgccgaaa acgaatttgt gctgaccaaa
     3721 gaaaaaccga aagtacccga aagtaccaaa gaatttgcca tcaatatgtc caaccgccgc
     3781 cactggctgc cccccgtcca acaatacatc gacgagctct tggcgggcga aaccggcacg
     3841 cgcggcaaaa actacaatat gcgctgggta gccagcatgg ttgccgaaat ccaccgcatc
     3901 ctgatgcgcg gcggcgtgtt catgtatccg caagacaaac gcgacccgtc caaacccggc
     3961 aaactgcgcc tgatgtacga agccaaccct atgagcctga tactcgaaca ggcgggcgca
     4021 tcggcaagca acgcccgtca ggacatactc ggcatccgtc ccgaaagcct gcaccagcgc
     4081 gtcgccgtca ttatgggcag cagcgaagaa gtggattacc tcaaccgcct gcattcaaaa
     4141 taaaaggcag cctgaccgat aaagaaaggc ggcagcgcgc atccgacgct gccgccgata
     4201 tttcgtttat agccttaaaa agatagaaaa ccacctttcc gaacggcaaa aatgccgtct
     4261 gaaaagtttt cagacggcat tttaaaaatt aaagcccgaa tttatttgcc cgctgctttt
     4321 tgcaggatgg cgtacaactc gtctttcagt ttcaatttgg cttttttcag ctcatcgatg
     4381 gtttccgcac cgctggtaac cggattgttg accagaccgg taattttatc gtccagctcg
     4441 ttgtgttcgt cgaacagacg ggcgaagcga gaattttcct gtttcaattt ggaaatcaaa
     4501 tcacggtatt ctgggaacat atcgctttcc tctcttggtt tacggttaaa aagtcaaatt
     4561 gaatctttac attaattata acaaagataa tatttttatc cagtgtttta tcggatacgg
     4621 caaaattttc ttttaacacc gccgtccggt tgataaaaac gttgacaata tcacgcttta
     4681 cgcataaatt ttcagccttt ccta
//
LOCUS       DAXHSF010000148         4585 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.148, whole genome shotgun sequence.
ACCESSION   DAXHSF010000148 DAXHSF010000000
VERSION     DAXHSF010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.148"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(12..422)
                     /locus_tag="ACFM8S_001722"
     CDS             complement(12..422)
                     /locus_tag="ACFM8S_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton exchanger"
                     /protein_id="HFC6314766.1"
                     /translation="MPISPVMMPPVTGVPNSHLLFDVAFAVVVLSLLIRGMTIPVMAR
                     LLKVAMPNKPEPKDTHDIWLAKKEIVRMSAFKVVAESEAEGHHPDTVEPISDSFDARC
                     FALIRNGIRIEMQSDTVLQAGIWRGTSCPTARSI"
     gene            complement(404..685)
                     /locus_tag="ACFM8S_001723"
     CDS             complement(404..685)
                     /locus_tag="ACFM8S_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314767.1"
                     /translation="MDWMGSLFLPGGALLFLSVVSTTLSARLGMPLLLVSPANVLDGA
                     AEALAIAAFLMLVARPPAVFGGLWKFNYSLREKAYISWLGLAEPCRFRR"
     gene            881..1607
                     /locus_tag="ACFM8S_001724"
                     /pseudo
     CDS             881..1607
                     /locus_tag="ACFM8S_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688345.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
     gene            1676..3214
                     /gene="lnt"
                     /locus_tag="ACFM8S_001725"
     CDS             1676..3214
                     /gene="lnt"
                     /locus_tag="ACFM8S_001725"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFC6314768.1"
                     /translation="MVSKLDKYWQHPALYWPLLILFAAATPFTFAPYYHFWLMPLIFG
                     AFVRLIELRPRFAVSSAYLFGLTAYTTQFYWIHTALHDVSGLPDLYAVPLTFLLPAYL
                     ALYPALCFWLWKKFTLPRGIKIGLVLPILWTLTEFARERFLTGFGWGAIGYSQITPDS
                     PLAGFAPLGGIHMVTLATAFLGVWLVLASDNTTRSGKRLLPIILIAALLAAGYTARQT
                     DFTRPDGSRSTVALLQGNIDQTLKWREDQVIPTIQKYYEQVGKTTADIVILPETAIPV
                     MRQNLPENILAKFAEQAQNNGSALAVGISQYTSDGNGYENAVINLTGYQENNQDGIPY
                     YAKNHLVPFGEYKPLPFLTTPLYKMMNMPLSDFRKGGGKQSALLMKNQKIAFNICYED
                     GFGDELIAAAKDATLLANASNMAWYGKSNAMYQHLQQSQARAMELGRYMVRATNTGAT
                     AIISPKGNIIAQAQPDTETVLEGHIKGYVGETPYMKTGSSWWLMGILTLAALILFIFR
                     NKEH"
     gene            3478..4341
                     /gene="rpoH"
                     /locus_tag="ACFM8S_001726"
     CDS             3478..4341
                     /gene="rpoH"
                     /locus_tag="ACFM8S_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFC6314769.1"
                     /translation="MNNAFALPAIQSGNGSLEQYIHTVNSIPMLSQEEETRLAERRIK
                     GDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYEPGRGARLF
                     SFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNALSPKEAQDIADD
                     LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYAL
                     QTEGLQNALAQLDDRSRRIVESRWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKL
                     RGFLTEEAEAV"
     gene            complement(4400..4546)
                     /locus_tag="ACFM8S_001727"
     CDS             complement(4400..4546)
                     /locus_tag="ACFM8S_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314770.1"
                     /translation="MLIHYINSANLSEKQLMRQCLVHVCFLISAIAMVWTDKIVYGTT
                     HKPH"
BASE COUNT         1132 a         1420 c         1049 g          984 t
ORIGIN      
        1 aatttaatcc actatatcga ccttgccgtc gggcaggatg taccacgcca aatccccgcc
       61 tgaagcacgg tgtcggactg catttctatt cggatgccgt tgcgtatcaa tgcgaaacag
      121 cgtgcgtcaa acgaatcgga aatcggttcg accgtatcgg gatggtgtcc ctccgcttca
      181 gattcggcaa ccactttaaa cgccgacatt ctgacaattt cctttttcgc cagccaaata
      241 tcatgcgtat ctttcggttc gggcttgttg ggcatggcaa ccttcaacag ccgcgccatc
      301 acaggaatcg tcattccccg aatcagcagc gacagcacca ccacggcaaa cgccacatca
      361 aacagcaggt gcgaattggg aacgcccgtt accggcggca tcatcaccgg cgaaatcggc
      421 acggctccgc caaacccaac caactgatat acgccttttc acgcaggctg taattgaatt
      481 tccacaaacc gccgaacact gccggcggac gcgcgaccag catcaggaac gccgcaatcg
      541 ccaaggcttc cgccgccccg tccaacacgt tggcaggaga aaccagcagc aaaggcattc
      601 ccaaacgtgc ggacaaagtg gtcgaaacca cgctcagaaa caacagtgcg ccacccggca
      661 ggaacagact gcccatccaa tccacgctaa cgattctccc ttaaaattta tgaaattaaa
      721 atcgaaagac tgtaacacgg cattttcttt tcacgaaact ttacttcacg tccgccaccg
      781 cgttcagacg gcattttcct gtttcatgcc gcctgaaacc gcccaaaccc gcgttctcct
      841 atataatgtc ggactttgcg ccgcatttca ggattcttta atggcatttg cctcactatt
      901 taccctgccg gacgacatca ccgccgtttt ggacgatgtt gccctgatga ccaaagccgc
      961 cgcaaagaaa accgccggca tggtcggcga cgatttgaca cccaatgcca accgggttac
     1021 gggcgtatcg gcagaacgcg agttgccgat tgtttggtcg atggcgaaag gttctttttc
     1081 caacaagccg gttttggtgc ctgcgcccct gctgctttcc gccttcctgc cgcaactcat
     1141 tacgccgctt ttgacggcag gcggcattta tctgtgcttt gaaggcgtag aaaagctgct
     1201 gcacaagttt ctgcaccgac atgacgcgca tgctcgtaat ggcggcaatc ggcatcggca
     1261 tgactgtttt ggtgtacgga atcgttgccg tcatcgtcaa actcgacgac ttaggcatgc
     1321 tgctgatgcg ccgcccacaa accttttcgc gcagtttggg gcaaatgctg actgccttta
     1381 tgccttgctt tatgcgcggt ttgagcgtgg tcggcacgct tgccatgttt cttgtcggcg
     1441 gcgtactggt cgcccacaac ctcggccttc tgcacgattt cctgcacgcg caacactggg
     1501 acgctggctg ggcggagtac ttcgccaacc tcgttgtcgg gctgctttcc ggttcgattg
     1561 cctgcgcccc cgccttgccg ctgatgaatc gtttcggcag gcattgattt ctttttcaaa
     1621 taccgatgcc gtttgaaaga tgttcagacg gtatcttccg aacagacaga tgaatatggt
     1681 ttccaaactg gacaaatact ggcagcaccc cgccctctac tggcctttgc tcatcctttt
     1741 tgccgccgcc acccccttta ccttcgcacc ctactaccac ttttggctga tgcccttgat
     1801 tttcggtgcc ttcgtccgcc tcatcgaact gcgtccgcgt tttgctgtct cttccgccta
     1861 cctgttcggc ctgaccgcat acacgacaca gttctactgg atacacaccg ccctgcacga
     1921 cgtttccggc ctgcccgacc tctatgccgt accgctgacc ttcctactcc ccgcctacct
     1981 tgccctttat ccggcactgt gtttctggct gtggaaaaaa tttaccctgc ctcggggcat
     2041 aaaaatcggt ttggtactgc ccatcctgtg gacgctgacc gagtttgccc gcgagcgttt
     2101 cctgaccgga ttcggctggg gcgcaatcgg ctactcccaa atcaccccgg acagcccgct
     2161 cgccggcttt gccccattgg gcggcatcca catggttaca ctggcaaccg cctttctcgg
     2221 tgtctggctg gttttggcga gtgataacac cacacgttcg ggcaaacgcc tgcttccaat
     2281 tatcctgatt gccgccctgc ttgccgcagg ctacaccgcc cgacaaaccg acttcacccg
     2341 ccccgacggc agccgcagca ccgtcgccct gcttcaaggc aacatcgacc aaaccctcaa
     2401 atggcgtgaa gaccaagtca tcccgaccat acagaaatat tacgaacagg tcggcaaaac
     2461 cactgccgac atcgtcatcc tgcccgaaac cgccatcccc gttatgcgcc aaaacctgcc
     2521 ggaaaacata ctggcaaaat ttgccgaaca ggcgcaaaac aacggcagcg cgctcgccgt
     2581 cggcatcagc caatacactt cggacggcaa cggttacgaa aacgccgtca tcaacctgac
     2641 cggttatcag gaaaacaatc aggacggcat cccctactac gccaaaaacc acctcgtccc
     2701 cttcggcgaa tacaaaccgc tgcccttcct gaccacgccg ctttacaaaa tgatgaatat
     2761 gcccctttcc gacttccgca aaggcggcgg taagcaatcc gccctgctga tgaaaaacca
     2821 aaaaatcgcc ttcaacatct gttacgaaga cggattcggc gacgaactga ttgccgccgc
     2881 caaagatgcc acactgcttg ccaatgccag caatatggcg tggtacggaa aatccaacgc
     2941 catgtaccag cacctccaac aatcgcaggc gcgggctatg gaactcggac gctatatggt
     3001 ccgtgccacc aacaccggcg caaccgccat catctccccc aaaggcaaca tcatcgccca
     3061 agcccaaccc gatacggaaa ccgtattgga aggacacatc aaaggctatg tcggcgaaac
     3121 gccctatatg aaaaccggca gttcatggtg gttgatgggc atattgaccc tagccgcact
     3181 gattcttttc atcttccgaa acaaagaaca ctgatacctg cacagcccgt ccggacgggc
     3241 tgtctttttt caaaacggct atttacatac aattacgtat gtaatataat tctgaaatcg
     3301 aattgagcca tcaggtttac aaaatcaatc aaacctgcct tacaaaattt tcctgtcttt
     3361 caaaccttag cgcatatctc ctgccaaaca tacaaaatac aagccaagca gtgatatact
     3421 gcatcccaac acagcaaacc gccgatacgc agtttcagcc gaaaggaaat gccccaaatg
     3481 aataacgctt tcgcattacc cgccatccaa agcggcaacg gcagcctcga acaatacatc
     3541 cacaccgtca acagcatccc gatgctgtcc caggaagaag aaacccgcct cgccgaacgc
     3601 cggataaagg gcgacctcaa cgccgccaaa caactcatcc tgtcccacct gcgcgtcgtc
     3661 gtttccatcg cgcgcggcta tgacggctac gggctgaatc aggcagacct gattcaggaa
     3721 ggcaatatcg gactgatgaa ggcggtcaaa cgctacgagc ccggtagagg cgcgcgtctg
     3781 ttttcatttg ccgtacactg gattaaagcc gaaatccacg agtttatttt gagaaactgg
     3841 cgtttggttc gcgttgccac caccaaaccg caacgcaagc tgtttttcaa tttacgcagt
     3901 atgcgtaaaa acctcaacgc cctgtctccc aaagaggcac aagacatcgc cgacgatttg
     3961 ggtgtcaaac tttccgaagt tctggaaatg gaacagcgca tgacgggaca cgacatcgcc
     4021 atcatggcag acaacagcga cgacgaggac agtttcgccc ccatcgactg gcttgccgac
     4081 cacgattccg aaccgagccg gcaactatcg aaacaggcgc attacgccct gcaaacagaa
     4141 ggtttacaaa acgctttggc gcaactggat gacaggagcc gccgtattgt ggaaagccgc
     4201 tggcttcaag acgacggcgg gctgacgctg caccagcttg ccgccgaata cggcgtatct
     4261 gccgaacgca tccgccaaat cgaagcaaaa gccatgcaga aactgcgtgg tttcctgacc
     4321 gaagaagccg aagcggtttg aaatatacgc aataaaatac ccgacaaatt gttttgtcgg
     4381 gtattttatt tggtaaacat caatgcggtt tgtgcgtcgt gccgtacacg attttatccg
     4441 tccaaaccat cgcaatcgcc gaaatcaaaa agcagacatg caccagacac tgccgcatca
     4501 gctgcttttc cgacagattg gcggaattga tatagtggat taacaaaaat caggacaagg
     4561 cggcgaagcc gcagacagta caaat
//
LOCUS       DAXHSF010000149         4521 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.149, whole genome shotgun sequence.
ACCESSION   DAXHSF010000149 DAXHSF010000000
VERSION     DAXHSF010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.149"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(16..237)
                     /locus_tag="ACFM8S_001728"
                     /pseudo
     CDS             complement(16..237)
                     /locus_tag="ACFM8S_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261815.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine catabolism dioxygenase TauD"
     gene            complement(236..343)
                     /locus_tag="ACFM8S_001729"
                     /pseudo
     CDS             complement(236..343)
                     /locus_tag="ACFM8S_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(389..1117)
                     /gene="pdxJ"
                     /locus_tag="ACFM8S_001730"
     CDS             complement(389..1117)
                     /gene="pdxJ"
                     /locus_tag="ACFM8S_001730"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFC6314771.1"
                     /translation="MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLR
                     EDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEG
                     GLDVLAQQEKIAGFTKILTDAGIRVSLFIDADDRQIQAAYDVGAPVVELHTGAYADAR
                     SHAEQLKQFERLQNGAHFAGDLGLVVNAGHGLTIHNVTPIAQILAIRELNIGHSLIAQ
                     ALFLGLPEAVRQMKEAMFRARLLP"
     gene            complement(1141..1932)
                     /gene="recO"
                     /locus_tag="ACFM8S_001731"
     CDS             complement(1141..1932)
                     /gene="recO"
                     /locus_tag="ACFM8S_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFC6314772.1"
                     /translation="MSEHRVNHEPVFLLASSPWRESSLRVEAFSRRYGRVALLARSAR
                     KRQSELRGVLVPFVPVSASWYGSQELKTLHRAEWIGGWPQPQGRALFSGLYVNELMLK
                     LTVREDPLPELYDALAEVMEAVCCKAAYIDDLRRFEWRLLNLLGVAPDLHADGTGGDI
                     LADKTYRLMPEEAVMPVCEDADALSHEAGAIVEGQSLIDLREGSFRTAESLQQALKIT
                     RLFIRHLLPEGLKSRQVLEQIRQFDRKETARETVPTSDGTASNAV"
     gene            complement(1966..3093)
                     /gene="pheA"
                     /locus_tag="ACFM8S_001732"
     CDS             complement(1966..3093)
                     /gene="pheA"
                     /locus_tag="ACFM8S_001732"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFC6314773.1"
                     /translation="MLECTINYCSGEIMSQTIDELLLPHRNAIDTIDAEILRLLNERA
                     QHAHAIGELKGTGAVYRPEREVAVLRRIQDLNKGPLPDESVARLFREVMSECLAVERP
                     LTIAYLGPQGTFTQQAAIKHFGHAAHTMACPTIDNCFKQVETRQADYLVAPVENSTEG
                     SVGRTLDLLAVTALQACGEVVLRIHHNLLRKNNGSTEGIAKVFSHAQALAQCNDWLGR
                     HLPNAERIAVSSNAEAARLVAESDDGTVAAIAGRTAAEIYGLDMVAECIEDEPNNTTR
                     FLVMGHHETGASGSDKTSLAVSAPNRAGAVASLLQPLTESGISMTKFESRPSKSVLWE
                     YLFFIDIEGHRRDAQIQTALERLGERASFVKVIGSYPTAVL"
     gene            complement(3132..4364)
                     /locus_tag="ACFM8S_001733"
     CDS             complement(3132..4364)
                     /locus_tag="ACFM8S_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HFC6314774.1"
                     /translation="MPSAHYPEMSEKLMAVLMAMLVTLMPFSIDAYLPAIPEMAQSLG
                     ADVHRIEQSLSLFMFGTAFGQVVGGSVSDIKGRKPVALTGLAVYCLAVAAIVFASSAE
                     QLLNLRVVQAFGAGMTVVIVGAMVRDYYSGRKAAQMFALIGIILMVVPLVAPMVGALL
                     QGLGGWQAIFVFLAAYSLVLLGLVQYFLPKPAVGGKIGRDVFGLVAGRFKRVLKTRAA
                     MGYLFFQAFSFGSMFAFLTESSFVYQQLYRVTPHQYAWAFALNIITMMFFNRITAWRL
                     KTGAHPQSILLWGIVVQFAANLSQLAAVLFFGLPPFWLLVACVMFSVGTQGLVGANTQ
                     ACFMSYFKEEGGSANAVLGVFQSLIGAGVGMAATFLHDGSATVMAATMTASTSCGIAL
                     LWLCSHRAWKENEQSEYL"
BASE COUNT         1012 a         1303 c         1242 g          964 t
ORIGIN      
        1 ataatcatcg cctcaatatt taatatccca aaatatagat ttaaaaccat cgcctttgtg
       61 gtttgtcagc attcctaatt gagatagaaa gtcaaataat ttcttgtttc tccggctgcc
      121 atcaagatgg tttagattaa gacctcgaac aatacaatac ccaatactct gtactgtatt
      181 gtgaatttct tctatcaaac tattattgtt tgcagataaa atatactttt gattcatata
      241 gtgaattaaa tttaaaccag tacggcgttg gcttgccttg ccgtactatt tgtactgtct
      301 gcggcttcgc cgccttgtcc tgatttaaat ttaatccact ataacatatc cctgtcgtga
      361 aatgctgtct gaaagggttt gcctgccctt acggcagcag ccttgccctg aacatcgcct
      421 ccttcatttg gcgcacggct tcgggcagtc cgaggaagag ggcttgggca atcagcgaat
      481 gcccgatgtt cagttcgcgg atggcgagga tttgggcgat gggggtaacg ttgtgtatgg
      541 tcagtccgtg tccggcgttg acgaccaagc ccaaatcgcc ggcaaaatgc gcgccgtttt
      601 gcagacgctc gaattgcttg agttgttcgg cgtggctgcg cgcgtcggca tacgcgcctg
      661 tgtgcagctc gacaacgggc gcgccgacat cataggcggc ttggatttgc ctgtcgtcgg
      721 catcgataaa gagagagact cggatgcctg cgtcggtcag gattttggtg aacccggcga
      781 ttttttcctg ttgcgccaat acgtccaagc cgccttcggt cgtgatttcc tgacgttttt
      841 caggcacgat gcacacgtct tccggcatca ctttaagcgc gttttcgagc atttcttccg
      901 tcaacgccat ttcaaggttc aggcgcgtgc ggatggcgtt tttgacggca aacacatccg
      961 cgtctttgat gtggcggcgg tcttcgcgca ggtgcatggt aatcaaatcc gcaccgtgcg
     1021 tttcggcaac cagtgccgcc tccacggggc tgggataagt cgtgccgcgc gcattgcgga
     1081 cggtagcgat gtggtcgatg ttgacaccta aaagcataat ctttcctttt atttctgcct
     1141 tcagacggca tttgaagccg tgccgtccga agtcgggacg gtttcccggg cggtttcttt
     1201 gcggtcaaac tgccgtatct gttccaacac ctgccgcgat ttcagcccct cgggcaacag
     1261 gtggcggata aaaagccgtg tgattttcaa tgcctgttgc aggctttcgg cggtgcggaa
     1321 actgccttcg cgcaaatcga tcaggctttg cccttcgaca attgcgccgg cttcgtgcga
     1381 aagcgcgtct gcgtcctcgc atacgggcat gaccgcctct tcgggcatca gccggtaggt
     1441 tttgtccgcc aaaatatcgc cgcccgtgcc gtcggcgtgc aaatcggggg caacgcccaa
     1501 caggttcagc agccgccact cgaaacggcg caagtcgtcg atataagcgg ctttgcagca
     1561 caccgcctcc atcacttccg ccaacgcgtc gtataactcg ggcaacggat cttcgcggac
     1621 ggtcaacttc agcatcaact cgttcacata caatccgctg aacaaagccc taccctgcgg
     1681 ctgcggccac ccgcctatcc attcggcgcg atgtagggtc ttcaactctt gcgaaccata
     1741 ccaagacgca ctcaccggca caaacggcac caacacgccg cgcaattcgc tctgccgttt
     1801 gcgcgcgctt cttgccaaaa gtgccacacg cccgtaacgg cggctgaacg cttcgacccg
     1861 caggctgctt tcgcgccagg gcgaggaagc caacaggaag acgggttcgt ggttgactcg
     1921 gtgttcggac atcatgcctg aaatgccgtc tgaacgctgc cgccgctaca aaacggcggt
     1981 cgggtacgaa ccgatgactt tgacgaacga agcgcgttcg cccaagcgtt ccaatgccgt
     2041 ctgaatctgc gcgtcccggc ggtgtccttc gatgtcgatg aagaacaggt attcccacaa
     2101 aacggattta ctcggacggc tctcaaactt ggtcatggaa atacccgatt cggtcagcgg
     2161 ttgcagcagc gaggcaaccg cgccggcgcg gttgggcgcg gaaacggcca gcgaagtctt
     2221 gtcgctgccg cttgcaccgg tttcgtgatg tcccatcacc aagaagcgcg tggtgttgtt
     2281 cggttcgtct tcgatgcact cggcaaccat atcgagtccg tagatttccg ccgccgtgcg
     2341 tccggcgatg gcggcaaccg taccgtcgtc cgattcggca accagccttg cggcttcggc
     2401 attgctggac acggcaatcc gttcggcgtt gggcaggtgt ctgcccaacc agtcgttgca
     2461 ctgcgccaac gcctgcgcgt gggaaaagac tttggcaatg ccttcggtgc tgccgttgtt
     2521 tttacgcaaa aggttgtggt ggatgcgcaa aacgacttcg ccgcacgcct gcaacgcggt
     2581 aacggcaagc aaatccaacg tgcgcccgac cgagccttcg gtcgagtttt ccacgggggc
     2641 gaccagatag tctgcctgac gggtttcaac ctgcttgaag cagttgtcta tggtcggaca
     2701 cgccatggtg tgcgcggcgt gtccgaaatg cttaatcgcc gcctgctggg taaacgtgcc
     2761 ctgcggcccc agataggcga tggtcagcgg gcgttcgacg gcgagacact cgctcatcac
     2821 ttcccgaaac aggcgtgcta ccgattcgtc gggcagcggg cctttgttca aatcctgaat
     2881 gcggcgcaac acggcgactt cgcgttcggg gcggtacact gcgcccgtgc ctttcagctc
     2941 gccgatggcg tgggcgtgtt gcgcacgttc gttgagcagg cgcaggattt cggcatcgat
     3001 ggtgtcgatg gcgttgcggt ggggaaggag gagttcgtcg atagtttggg acataatttc
     3061 gcctgaacag taatttatcg tgcattctaa catcaaatgc cgtctgaaac ggtttcagac
     3121 ggcattttcc gttaaaggta ttcgctctgt tcgttttcct tccacgcccg gtgcgaacac
     3181 agccacagaa gcgcaatgcc gcaagaggtg gacgcggtca tcgttgccgc catcacggtt
     3241 gccgaaccgt cgtgcaagaa ggtcgccgcc atacccaccc ccgcgccgat taaggactgg
     3301 aacacgccca acacggcgtt cgcgctgccg ccctcttctt tgaaataaga cataaagcat
     3361 gcctgcgtgt ttgcaccgac caagccctgc gtaccgacgg aaaacatcac gcacgcaacc
     3421 agcagccaaa acgggggcaa cccgaaaaac agcacggcgg cgagttggga caggttggcg
     3481 gcaaactgga cgacaatccc ccatagcagg atgctttgcg gatgcgcgcc ggttttgagc
     3541 cgccacgcgg taatgcggtt gaaaaacatc atcgtgatga tgttgagtgc aaacgcccaa
     3601 gcgtattgat gaggcgtaac acggtaaagc tgctggtaca cgaaggaaga ttcggtcaga
     3661 aaggcgaaca tcgaaccgaa gctgaatgcc tgaaaaaaca gataacccat cgcggcacgg
     3721 gttttcaata cgcgtttgaa ccgcccagcc accagcccga acacatcccg tccgattttg
     3781 ccgccgacgg caggcttggg caggaaatac tgcaccaaac cgagcagcac cagcgaatac
     3841 gccgccagaa aaacaaaaat cgcctgccag ccacccaagc cctgcaacag tgcgccgacc
     3901 atgggtgcga ccagcggcac aaccatcaaa atgatgccga taagggcaaa catctgggcg
     3961 gctttgcgtc cggaataata atcgcgcacc attgcgccga cgatgaccac agtcatgccc
     4021 gcaccgaatg cctgcacgac gcgcaggttg aggagctgtt cggcactcga agcaaatacg
     4081 atggcggcaa cggcaaggca gtagacggcc agtccggtca gcgcgacggg tttgcgccct
     4141 ttgatgtcgg acaccgaacc gccgaccacc tgtccgaacg ccgtgccgaa cataaacaaa
     4201 ctcaggctct gttcgatgcg gtggacatcc gcacccagcg actgcgccat ttcgggaatc
     4261 gcgggcaggt aggcatcgat ggaaaacggc atcagcgtaa ccagcatcgc catcaaaacc
     4321 gccatcagtt tttcgctcat ttcaggataa tgagcagaag gcataaattc tcgtccgatt
     4381 cgcaaacaaa ccgcccggcg gcggaaaaca caatacgcgg aaacggaaaa ttttaacaca
     4441 ccggcacaaa caggcggcaa caaaagcccc ggcaaaaccg gacgaaccgg aacgatatag
     4501 tggattaaca aaaaccagta c
//
LOCUS       DAXHSF010000150         4450 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.150, whole genome shotgun sequence.
ACCESSION   DAXHSF010000150 DAXHSF010000000
VERSION     DAXHSF010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.150"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(6..81)
                     /locus_tag="ACFM8S_001734"
     tRNA            complement(6..81)
                     /locus_tag="ACFM8S_001734"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46..48),aa:Val,seq:tac)
     gene            complement(151..2127)
                     /locus_tag="ACFM8S_001735"
     CDS             complement(151..2127)
                     /locus_tag="ACFM8S_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFC6314775.1"
                     /translation="MNEFSLAPIVIVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPG
                     MLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGVTMLSVM
                     GILMLTGVPFNWAFAVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVV
                     PLMILIPALAGGGDGNIWAALGLAFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSEL
                     FMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVEDDIRPFRDILLGFF
                     FITVGMKLDIQALIGGWRQVLMLLAMLLVLKALVVFAIAFKMKHSVGDSLKTALYLAQ
                     GGEFGFVMLAIAGQLDMVSPEWEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDM
                     KSLDLHSMLVETMSKSDHVLIVGFGRGGQTVGRVLAQEDIPYFALDLDIARVQVARSA
                     GEPVSFGDAKRREVLEAAGLGRAKMVVVTLNNMHETQHVLDNVLSMYPNMPVYVRATN
                     DDYVKTFTDIGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEG
                     LFVGSDDEAGFGENGETVRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIE
                     NPDASFTLEGGDVLVVAGKKEEIISFENWSLQGI"
     gene            2276..3115
                     /locus_tag="ACFM8S_001736"
     CDS             2276..3115
                     /locus_tag="ACFM8S_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HFC6314776.1"
                     /translation="MTATAAAIDRLLPQTQCRECGYDGCLPYAQAVATGEAYNLCAPG
                     GETVIRDISALLGKPLVAPAKTQAKALARIDETACIGCTACIRACPADAIMGAGKLMH
                     TVIADECTGCGLCVAPCPVDCIHMQPVADTVLPRARRFSLSADSRFAAAEHARARYLK
                     RNERKQREADERKAMLAEREAAVRNARPQTPDTPKKPAFNPADLIAKAMAKAQTQQDR
                     LAAADNRKDYQAKQIAEARERAELRRAQRDMKYGSDSEKAAALEYLKQYKAKQEAAQN
                     TAS"
     gene            3315..4346
                     /gene="gap"
                     /locus_tag="ACFM8S_001737"
     CDS             3315..4346
                     /gene="gap"
                     /locus_tag="ACFM8S_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFC6314777.1"
                     /translation="MGIKVAINGYGRIGRQVLRAIYDYQIQDQLQIVAVNASGSLETN
                     AHLTKFDTVHGRFEADVSHDGGNLIVNGDKIPFFSTRNPAELPWKELGVDLVMECTGA
                     FTSKEKAKIHLKSGAKKVLISAPGGDDVDATVVYGVNDSILTADMTVVSNASCTTNCL
                     SPVAKVLSESVGIVKGTMTTIHALTNDQTVTDVRHKDLRRARSGVENMIPTKTGAAKA
                     VGLVLPELKGRLDGLAIRVPTVNVSLVDLSFQAARDTTVEEINALMKAASEAGALKGV
                     LGYNTLPLVSMDFNHTTEASHFDATLTKVVDGNMVKVFAWYDNEWGFSCQMLNTARRM
                     FELEVRPLK"
BASE COUNT         1128 a         1487 c         1034 g          801 t
ORIGIN      
        1 aaacctggtg ggtgatgacg gagtcgaacc gccgacattc tgcttgtaag gcagacgctc
       61 taccaactga gctaatcacc cttgcgattt gcgaaagcgt tattaaacca aataaccgga
      121 aatggcgcaa gccttatttc ggcatttcat ttatattccc tgcaaactcc agttttcaaa
      181 agagataatt tcttcttttt tgcctgcgac caccaacacg tcaccgcctt ccaatgtaaa
      241 cgaggcatcc gggttttcaa tccggccggt gcggcggcgg acgaacaaga gtttgatgcc
      301 gtaagccgcc atcggaagcg tgccgactgt tttgccgacg gcgtatgctt ctgcagccaa
      361 aggaaaggcg tgacggacgg tttcgccgtt ttcgccgaat cctgcctcat catcactacc
      421 gacaaacagt ccctccaacg cggcataacg gctgtggcgg atatttgcca tcgtctgata
      481 gacgtgccga tacgacgcgc cgttgcctaa catcgcatag cctgccagca cgagtccggt
      541 ttctttggtg tccgacacgg cttcttccgc acctatatcg gtaaacgttt tcacataatc
      601 gtcgttggtg gcgcgcacat atacgggcat attgggatac atggacagca cattgtctaa
      661 aacgtgttgc gtttcgtgca tattgttgag cgtaaccacc accattttcg cccgtcccag
      721 accggcggct tccaatactt ccctgcgttt cgcatcgccg aacgacaccg gttcgcccgc
      781 acttctggca acctgcaccc gcgcgatgtc caaatcgagc gcgaaatacg gaatatcctc
      841 ttgggcaagg acgcgtccga ccgtctgccc gcccctgccg aagccgacaa tcagcacatg
      901 gtcggacttg ctcatggttt ctaccagcat actgtgcaga tcgagcgact tcatgtccca
      961 gcttgacttg accaaacgcc cgaccagcgc atcgctgccg cccaagagga agggcgcgat
     1021 aatcatcgac agcagaaccg ccgccgtcgc cgcctgttcc cattctggcg aaaccatatc
     1081 aagctgcccg gcaatggcca gcatcacgaa gccgaactcg ccgccttgcg cgagatacaa
     1141 agccgttttg aggctgtcgc cgaccgaatg tttcattttg aaggcaatgg caaacacaac
     1201 cagtgccttc aacaccagca gcattgccaa cagcatcaat acctgccgcc agccgccgat
     1261 caatgcctga atgtccagct tcatgccgac cgtgataaag aaaaagccga gcaaaatatc
     1321 gcggaacggg cggatgtcgt cttcgacttg gaaacggtat tccgtttccg aaagcagcat
     1381 gccggcaacg aatgcgccca acgccataga caaaccttcc agctcagtca gataagccac
     1441 acccaaggtt accagcagca cattgatcat aaagagttcg gacgatttgc gttttgccac
     1501 catcctgaac catcgcgaca taattttgct gccgacgaaa aacagcagcc ccagcgtcag
     1561 cagcattttt gcaaacgcca aacccaaggc cgcccaaata tttccgtccc ctccgcccgc
     1621 cagcgcggga atcagaatca tcagcggcac gacggcgatg tcctgcatca gcagcacgcc
     1681 catcgccatc tgaccgtgcg gctgccccaa ttccgtcttt tccgacaaaa tccggcttac
     1741 aatcgccgtg gacgacatcg ccaacgcgcc cgacacggca aacgcccaat tgaacgggac
     1801 gcccgtcagc atcagtatgc ccattaccga cagcatcgta acgccgacct gcaaaccgcc
     1861 cagcccaaac accagccgcc tcatcgccct caacttgggc agcgagaact ccaaaccgat
     1921 gctgaacatc aggaacacaa tcccgatttc gcccaaataa tccgtcgcat ggcttttcgg
     1981 aatcaggctg agcataccgg gccccgccaa aaagcccacc agcaggtagc ccagcatgga
     2041 gggaatgttg aacttgcggc acaggatcac cgtaatgacc gacaccagca aaacaatcac
     2101 aataggggca agcgaaaatt cgttcataga ccgtccgaac aggaaaatac agaaaaatgc
     2161 cgtctgaaac ggcatacgcc gccgcattat aacaaaacac cgcgcaccat ccgaaacggg
     2221 cgcggcatac aattctgcta aaatacgccc tttcgatttt gagccgcaca ccgacatgac
     2281 cgccacagcc gccgccatag accgcctgct tccccaaacc caatgccgcg aatgcggcta
     2341 cgacggctgc ctgccctacg cacaggcagt cgcaacaggc gaagcgtaca acctctgcgc
     2401 cccgggcgga gaaaccgtca ttcgggacat ttccgccctg ctcggcaaac cccttgtcgc
     2461 acctgccaaa acccaagcca aagcactcgc ccggatagac gaaaccgcct gtatcggctg
     2521 caccgcctgc atccgcgcct gccctgccga tgccattatg ggcgcgggca aacttatgca
     2581 caccgtcatc gccgacgaat gcaccggctg cggactctgc gtcgccccct gccccgtcga
     2641 ctgcatccat atgcagcccg ttgccgacac cgtcctgccc cgcgcgcgcc gcttcagcct
     2701 gtccgccgac agccgttttg ccgccgccga acacgcgcgc gcgcgctacc tcaaacgcaa
     2761 cgaacgcaaa cagcgcgaag ccgacgaacg caaggccatg cttgccgaac gcgaagccgc
     2821 cgtccgtaac gcgcgtccgc aaacacccga cacaccgaaa aaaccggcgt ttaaccctgc
     2881 cgacctcatc gccaaagcca tggcaaaagc gcaaacccaa caagaccgcc tcgccgccgc
     2941 cgacaaccgc aaagactatc aggcgaaaca gatagccgaa gcccgcgaac gcgccgagtt
     3001 gcgccgcgcc caacgcgata tgaaatacgg cagcgacagc gaaaaagccg ccgccctcga
     3061 atatctcaaa caatacaaag ccaaacagga agccgcacag aataccgcct cctgaccctt
     3121 ccctgatatg ccgtctgaaa accgtttcag acggcatttt atcaagctct ccgtccgccg
     3181 cacccgtgcc gtccgcatct taccgcccac ccttccggcc gcgccgtttt caataaaata
     3241 ttaattacac gccactacaa atttgctata atccgccccg aaaatctacc caaccctcaa
     3301 caaaggaacg aaccatgggc atcaaagtcg ccatcaacgg ctacggacgc atcggccgcc
     3361 aggttttgcg cgccatctac gattatcaga ttcaagacca actccaaatc gtcgccgtca
     3421 acgccagcgg cagccttgaa accaacgccc atctgaccaa attcgacacc gtgcacggac
     3481 gctttgaagc cgacgtatcc cacgacggcg gcaacctcat cgtcaacggc gacaaaatcc
     3541 ccttcttctc gacccgcaac cctgccgaac tgccgtggaa agaactcggt gtcgatttgg
     3601 tcatggaatg caccggcgcg ttcaccagca aagaaaaagc caaaatccac ctcaaaagcg
     3661 gcgcgaaaaa agtcctcatt tccgcaccgg gcggcgacga tgtcgatgca accgtcgtat
     3721 acggcgtgaa cgacagcatc ctgaccgccg acatgaccgt cgtttccaac gcttcctgca
     3781 ccaccaactg cctctcgccg gttgccaaag tgttgagcga aagcgtcggc atcgtcaaag
     3841 gcacgatgac caccatccac gcgctgacca acgaccaaac cgttaccgac gtgcgccaca
     3901 aagacctgcg ccgcgcccgc agcggcgtgg aaaacatgat tccgaccaaa accggcgcgg
     3961 caaaagccgt cggtttggtg ctgcccgaat tgaaaggcag gctcgacggg cttgccatcc
     4021 gtgtgccgac cgtcaacgta tccctggtag atttgagctt ccaagccgcg cgcgacacca
     4081 ctgtcgaaga aatcaacgca ctgatgaaag ccgcctcgga agcaggcgcg ctcaaaggcg
     4141 ttttgggcta caacaccctg ccattggttt ccatggactt caaccacacc accgaagcca
     4201 gccacttcga cgccacactg accaaagtcg tcgacggcaa tatggtcaaa gtgttcgcgt
     4261 ggtatgacaa cgaatggggc ttcagctgtc aaatgctgaa cactgcacgc cgcatgttcg
     4321 aacttgaagt gcgcccgctc aaataagcaa taaaccgtca aacaaaatgc cgtctgaaac
     4381 ccgatgtttt caagttccag acggcatttt tcatttttca ccgtgatttt acccggctgt
     4441 cgtcatttcc
//
LOCUS       DAXHSF010000151         4446 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.151, whole genome shotgun sequence.
ACCESSION   DAXHSF010000151 DAXHSF010000000
VERSION     DAXHSF010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.151"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(63..1205)
                     /locus_tag="ACFM8S_001738"
     CDS             complement(63..1205)
                     /locus_tag="ACFM8S_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFC6314778.1"
                     /translation="MNTLYTLFATCPRGLETVLSQELGSLGCTDVQAFDGGVSCRGGL
                     EQVYAANLHSRTASRILLRLTKGTYRNERDIYKLAKNINWFNWFTLQQTFKVKVEAKR
                     ANVKSIQFVGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLDERNVEIFIDTSGE
                     ALFKRGYRLDTGEAPLRENLAAGLLLSAGYDGTQPFQDPFCGSGTIAIEAAWIAARRA
                     PGMMRRFGFEKLQNFDKTLWSDLRRRAEAQVKPAFAPISGSDSDRRIIQTALANARSA
                     GVDDIVSFSVADAQSVRPNGENGIMVSNPPYGVRLEEVRALQALYPQLGTWLKKYYAG
                     WLAAMFTGDREMPKFMRLSPKRKIPLYNGNLDCRLFLIDMVQGSNR"
     gene            complement(1254..1676)
                     /locus_tag="ACFM8S_001739"
     CDS             complement(1254..1676)
                     /locus_tag="ACFM8S_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314779.1"
                     /translation="MNKNRKLLLAALLLIAFAAVKLVLLQWWQAQQPQAVAAQCDLTE
                     GCTLPDGSRVRAAAVSTKKPFDIYIEHAPVGTEQVSISFSMKNMDMGFNRYMFERQPS
                     GTWQAVRIRLPICVEGRRDFTADITIGSRTFQTAFTAE"
     gene            complement(1669..2478)
                     /locus_tag="ACFM8S_001740"
     CDS             complement(1669..2478)
                     /locus_tag="ACFM8S_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFC6314780.1"
                     /translation="MIQDTRPLIRVVAGILLDSDGNYLLSSRPEGKPYAGYWEFAGGK
                     VEAGETDFQALQREFEEELGIRILAATPWLTKIHSYEHARVCLKFLWVNPDQWTGEPQ
                     SREGQEWSWQKAGDFTVAPMLPANGALLRSLSVPRRLYGSLKTGLHGENSMGAYRVLP
                     LGSAEGSGANVLMEAAQWQDRPEYAGSVWMMVQTREQWRQAQEKGADVVVWRVGNAAQ
                     AAAVDEVLKHGVSVPLVLAANGQTVARYGKLWLGLGAHVVVRDETIGKNHE"
     gene            complement(2560..2937)
                     /gene="acpS"
                     /locus_tag="ACFM8S_001741"
     CDS             complement(2560..2937)
                     /gene="acpS"
                     /locus_tag="ACFM8S_001741"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFC6314781.1"
                     /translation="MIYGIGTDIVSLKRIIRLNKKFGQAFAGRILTPEELLEFPQAGK
                     PVNYLAKRFAAKEAFAKAVGTGIRGAVSFRNIGIGHDALGKPEFFYGPALSKWLEEQG
                     ISRVSLSMSDEEDTVLAFVVAEK"
     gene            complement(3137..3904)
                     /locus_tag="ACFM8S_001742"
     CDS             complement(3137..3904)
                     /locus_tag="ACFM8S_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFC6314782.1"
                     /translation="MQEIMQSIVFVAAAILHGITGMGFPMLGTTALAFNMPLSKVVAL
                     VALPSLLMSLLVLCSNNKKGFWQEIVYYLKTYKLLAIGSVVGSILGVKLLLILPVSWL
                     LLLMAIITLYYSVNGILNVCAKAKNIQVVANNKNMVLFGFLAGIIGGSTNAMSPILLI
                     FLLSETENKNRIVKSSNLCYLLAKIVQIYMLRDQYWLLNKSEYGLIFLLSVLSVIGLY
                     VGIRLRTKISPNFFKMLIFIVLLVLALKIGHSGLIKL"
     gene            complement(3914..>4446)
                     /locus_tag="ACFM8S_001743"
     CDS             complement(3914..>4446)
                     /locus_tag="ACFM8S_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HFC6314783.1"
                     /translation="DITFSEDVGECPLHSDSSFSENPESYLVMYVVKSANDGGNSLFL
                     SSSDIVNQLSKTETGKKHLKTLTGNLYPFKTPASFDKKQGVRWGNILSVNTQMIRFRS
                     DCIYKGIEENRNKVSKEMVLALDYLVNVIKNASDIQEFSAQDDGLIIIDNVNGLHART
                     DYTDKNRHYIRARITV"
BASE COUNT         1102 a         1106 c         1078 g         1160 t
ORIGIN      
        1 tcggccggat attaaaaaaa gccgtctgaa catttttcag acggcatttt tgtacacttt
       61 cctcaacggt tcgatccctg caccatatca atcaggaaca ggcggcaatc gaggttgccg
      121 ttataaagcg gaattttgcg ttttggcgac aggcgcatga atttgggcat ttcccgatct
      181 ccggtaaaca ttgccgccaa ccagcctgcg taatatttct tcaaccacgt ccccaactgc
      241 ggataaagcg cctgcaaggc gcggacttcc tcaaggcgca cgccgtaggg cggattggac
      301 accataatgc cgttttcgcc gttcggacgg acggattgcg cgtcggcaac gctgaaggaa
      361 acgatgtcgt ccaccccggc actgcgtgcg ttggccaatg ccgtctgaat gatgcggcgg
      421 tcgctgtcgc tgcccgaaat cggtgcaaaa gccggtttaa cctgcgcttc ggcacggcgg
      481 cgcaaatccg accacagcgt tttatcgaaa ttttgcagtt tttcaaaacc gaaacggcgc
      541 atcatacccg gcgcgcggcg ggcggcaatc caagcggctt cgatagcaat cgtgccgctg
      601 ccgcaaaacg ggtcttgaaa cggctgcgtg ccgtcgtagc ctgccgagag cagcaatccg
      661 gcggcaaggt tttcgcgcag cggggcttcg ccggtatcga gacggtagcc gcgtttgaac
      721 agggcttcgc ccgaagtgtc gatgaagatt tcgacattgc gttcgtccaa aaaggcgtgg
      781 atgcgaacat cgggcgcggc tttgtccacg ctcggacgtg cgtcgtaaat gtcgcggaaa
      841 gcgtcgcaga cggcatcttt gacggtcagt ccgacaaatt ggatgctctt aacgttggca
      901 cgctttgcct cgactttgac tttgaacgtc tgctgtaaag taaaccaatt aaaccagttg
      961 atatttttgg cgagtttgta gatgtcgcgc tcattgcggt atgtcccttt ggtcaggcgc
     1021 agcaggatac ggctggcagt acgcgaatgc aggttggcgg cgtaaacctg ttccaatccg
     1081 ccgcggcagg aaacgccgcc gtcaaacgct tgaacatcgg tacagccgag gcttccgagt
     1141 tcttgagata aaacggtttc caagccgcgc gggcaggtgg cgaaaagtgt atataaagta
     1201 ttcataacgt gtcctttccg gacggatgtt ccggcaatgg cggattgaaa ggtttattcg
     1261 gcggtaaatg ccgtctgaaa tgtccggctg ccgattgtaa tgtccgccgt aaaatcgcgc
     1321 ctgccttcga cacagatggg caggcggatg cgtactgcct gccaagtccc cgacggttgc
     1381 cgctcgaaca tatagcggtt gaaacccata tccatatttt tcatactgaa gctgatgctg
     1441 acctgttccg tgccgacggg cgcgtgttcg atataaatat caaacggttt tttggttgaa
     1501 acggcggcgg cgcggacgcg gcttccgtcc ggcagcgtgc aaccctcggt caaatcgcat
     1561 tgcgccgcca cggcttgcgg ctgctgcgcc tgccaccatt gcaacaaaac gagcttgacg
     1621 gcggcaaagg caatcagcag cagtgcggca agcagtaatt tacggttttt attcatgatt
     1681 cttccctatt gtttcatccc ttaccaccac gtgcgccccc aatccgagcc atagttttcc
     1741 ataacgtgca accgtctgtc cgtttgctgc aagtacgagc ggcacggata cgccgtgctt
     1801 caacacttcg tctaccgctg cggcttgtgc ggcattaccg actcgccaga cgaccacatc
     1861 cgcgcccttt tcctgcgcct gccgccattg ttcgcgggtc tgcaccatca tccacacgct
     1921 gccggcgtat tcgggtctgt cctgccattg cgccgcctcc atcaaaacgt tcgcaccgct
     1981 tccctctgcc gaacccaaag gcaggacgcg gtacgcgccc atactgtttt ctccgtgcaa
     2041 acccgttttc aggctgccgt acaaacggcg cgggacggac agcgaacgca aaagcgcgcc
     2101 gttggcgggc agcatggggg caacggtaaa atcacccgcc ttctgccaag accattcctg
     2161 cccttcgcgg gattgcggtt cgcccgtcca ttggtcgggg ttgacccata ggaatttcag
     2221 gcagacgcgg gcgtgttcgt aggaatggat tttggtcaac caaggcgtgg cggcgaggat
     2281 gcggatgccg agttcttctt caaactcgcg ttgcagggct tggaagtcgg tttcgcccgc
     2341 ttcgaccttg ccgccggcaa attcccaata tccggcatag ggtttgcctt cggggcgcga
     2401 gctgagcagg tagttgccgt ctgaatcgag caggatgccg gcaacgacgc ggataagggg
     2461 gcgggtgtct tgaatcatgg cgggtacgcg gttggtcggt cggagtgcaa atgagggatg
     2521 ggcaatgccg tcaacgggtt ttccgcattt cagacggcat tatttttcgg caacgacaaa
     2581 cgccaatacg gtgtcttctt cgtcgctcat gctgaggctg acgcggctga tgccttgttc
     2641 ctccagccat ttggacaggg cggggccgta gaaaaattcg ggcttgccca atgcgtcatg
     2701 cccgatgccg atgttgcgga aggaaaccgc gccgcgtatg cccgtgccga cggctttggc
     2761 aaaggcttct ttggcggcaa agcgtttggc gaggtagttg acgggtttgc ccgcttgcgg
     2821 aaattcaagc agctcttctg gagtgaggat gcgcccggca aacgcctgtc cgaatttttt
     2881 gtttaagcgg atgatgcgct tgagggaaac aatgtctgtg ccgatgccgt aaatcatatt
     2941 tgcgctcctt cgcccttggt tgcgggtagt gatgaatgtg atgatgaatg ccgtcaattt
     3001 cttggggcgt taaaatggcg tgaatatcca cttttggggt tttgtcggta acaattttca
     3061 ctgtaacctt ttgcatttga aattctaaaa catcgtgcca attatttaat aaggagttaa
     3121 ggcatttaat aatgaattaa agtttgatta aacccgaatg cccgattttc agagccaata
     3181 ccaataaaac aataaaaatt aacattttaa aaaaatttgg gctaatctta gtccttaacc
     3241 gaattccaac atacaatcca ataacagaca atacggacag taaaaatatt aaaccgtatt
     3301 cactcttatt taataaccaa tactggtctc ttagcatata tatttgaaca attttcgcca
     3361 aaagatagca tagattgctt gattttacga tacgattttt attttctgtt tcgctaagca
     3421 aaaatattaa caatatggga gacatggcat tggttgaacc gccgatgatg cctgccaaaa
     3481 acccaaaaag aaccatattc ttattattgg caactacttg aatatttttt gcttttgcac
     3541 atacatttaa aataccattg acagaataat acaatgtaat gattgccatc agtaaaagca
     3601 gccaagacac tggaagtatc aaaagcaact tcacccccaa aatgctgcca acgacgctgc
     3661 cgatagcaag caatttatag gtttttaaat aataaacaat ctcttgccaa aaaccctttt
     3721 tgttattgct gcatagaacc aacaagctca ttaacaggct tggtaatgcc accaaggcaa
     3781 caaccttaga caatggcatg ttaaaagcca atgcggttgt accgagcatc ggaaatccca
     3841 tgcctgtaat tccgtgcagt attgcggcag caacaaaaac gatagattgc attatttctt
     3901 gcataaccgt cctttataca gtaattcttg ctctaatata atgcctgttt ttatccgtat
     3961 aatcagttct ggcatgcaaa ccattgacat tgtcaataat aatcaaacca tcatcttgtg
     4021 cagaaaattc ttgaatatca ctcgcatttt ttataacatt tacaagataa tcaagtgcaa
     4081 gtaccatttc ctttgatact ttatttctat tttcttcaat acctttatag atacaatcac
     4141 ttctaaatct aatcatttga gtattgaccg ataagatatt accccatctc acaccttgtt
     4201 ttttatcaaa tgatgctggt gttttaaatg gatataaatt gcccgttaat gtttttaagt
     4261 gttttttacc ggtttctgtt ttagataact gattgacaat atctgatgaa cttaaaaata
     4321 gggaattacc tccatcattg gctgatttta ctacatacat aaccaaataa ctttccgggt
     4381 tttcactaaa agatgaatca ctatgaagtg gacattctcc aacatcttcc gagaaagtta
     4441 tatcat
//
LOCUS       DAXHSF010000152         4418 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.152, whole genome shotgun sequence.
ACCESSION   DAXHSF010000152 DAXHSF010000000
VERSION     DAXHSF010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.152"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(166..411)
                     /locus_tag="ACFM8S_001744"
     CDS             complement(166..411)
                     /locus_tag="ACFM8S_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314784.1"
                     /translation="MLKSIELNSHIRNRLAEYLKGRGMDFQTAMQEEKGNKEIASIIH
                     GGLPVLVRKLYSEQKMQKFFWEKRDLIADYISRRMQG"
     gene            523..951
                     /locus_tag="ACFM8S_001745"
     CDS             523..951
                     /locus_tag="ACFM8S_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HFC6314785.1"
                     /translation="MMFSWFKLFHLFFVISWFAGLFYLPRIFVNMAMIDVPRGNPEYV
                     RLLGMAVRLYRFMSPLGFGAVVFGAAIPFAAGWWGSGWVHVKLCLGLMLLAYQLYCGV
                     LLRRFQDYSNAFSHRWYRVFNEIPVLLMVAALYLVVFKPF"
     gene            963..1439
                     /locus_tag="ACFM8S_001746"
     CDS             963..1439
                     /locus_tag="ACFM8S_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFC6314786.1"
                     /translation="MPIEIERRFLIGNDNWRQYADEPLLLRQGYLSVEKERTVRVRIA
                     GKQAWLTLKGYISEISRSEFEYEIPLADAEKMMETMCPFKMEKWRYPVRWGGSLFEID
                     VFLGDNAPLVVAEIELPDENADFDRPDWLGREITADGMFTNAYLSRHPFSSWKNAV"
     gene            complement(1789..2277)
                     /locus_tag="ACFM8S_001747"
     CDS             complement(1789..2277)
                     /locus_tag="ACFM8S_001747"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFC6314787.1"
                     /translation="MLKITLIAACAENLCIGAGNAMPWHIPEDFAFFKAYTLGKPVIM
                     GRKTWESLPVKPLPGRRNIVISRQADYCAAGAETAASLEAALALCAGAEEAVIMGGAQ
                     IYGQAMPLATDLRITEVDLSVEGDAFFPAIDRTHWKEAERTERRVSSKGTSYAFVHYL
                     RY"
     gene            complement(2345..3400)
                     /gene="aroG"
                     /locus_tag="ACFM8S_001748"
     CDS             complement(2345..3400)
                     /gene="aroG"
                     /locus_tag="ACFM8S_001748"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFC6314788.1"
                     /translation="MTHHYPTDDIKIKEVKELLPPIAHLYELPISKEASGLVHRTRQE
                     ISDLVHGRDKRLLVIIGPCSIHDPKAALEYAERLLKLRKQYENELLIVMRVYFEKPRT
                     TVGWKGLINDPHLDGTFDINFGLRQARSLLLSLNNMGMPASTEFLDMITPQYYADLIS
                     WGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLKIAIDAIGAASHSHHFLSVTKAG
                     HSAIVHTGGNPDCHVILRGGKEPNYDAGHVSEAAEQLRAAGVTDKLMIDCSHANSRKD
                     YTRQMEVAQDIAAQLEQDGGNIMGVMVESHLVEGRQDKPEVYGKSITDACIGWGATEE
                     LLALLAGANKKRMARAG"
     gene            complement(3562..3789)
                     /locus_tag="ACFM8S_001749"
     CDS             complement(3562..3789)
                     /locus_tag="ACFM8S_001749"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314789.1"
                     /translation="MGNTRIPIKSFFKQLVLFVFNIFLAYEILPDVQAESAAGCSVPD
                     CRGNGKTDVPCGFVRMNGGYCFYQSVSFSLE"
     gene            4067..4324
                     /locus_tag="ACFM8S_001750"
     CDS             4067..4324
                     /locus_tag="ACFM8S_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314790.1"
                     /translation="MTTQMGASRDVSKSAKGWSVGIGLNVGKQLTDSVDLEFDPYYRH
                     KTICKPREIVLDGDKTKMGRSKSNEYGFRVAATFYSQLKSK"
     gene            complement(4306..>4418)
                     /locus_tag="ACFM8S_001751"
     CDS             complement(4306..>4418)
                     /locus_tag="ACFM8S_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HFC6314791.1"
                     /translation="LNPIEKVWANIKRYLRTVLSDYARFDDALLSYFDFN"
BASE COUNT         1060 a         1144 c         1031 g         1183 t
ORIGIN      
        1 acaagccgga ttaccgcctg cgcgggaatg acggctcaaa agttacgaaa cgaaaaataa
       61 ccgaaaccag acaagtcgga ttcccgcgcg ggcgggaatg acgataagcg taatttcaat
      121 ctgccatatt gtcttttcac ttgcctgaaa acagatttca gccgcctatc cctgcatccg
      181 gcggctgatg tagtcggcaa tcaaatcccg cttttcccaa aaaaatttct gcattttttg
      241 ttcggaatac agtttgcgaa ccagtacggg caggccgcca tgaataattg aggcaatttc
      301 tttgttgcct ttttcttcct gcattgccgt ctgaaaatcc atacccctgc ctttcagata
      361 ttctgcaagg cggttgcgga tgtgggaatt gagttcgatg gatttgagca ttttgcctcc
      421 gttttgcggt aaggatttat tcgatttgtt tatccgcctg attttatcac gtttcccaag
      481 cggtttgcct gtacaatacc gcttttcggc agggtgtggg cgatgatgtt ttcttggttc
      541 aagctgtttc acttgttttt tgtcatttcg tggtttgcag ggctgtttta cctgccgagg
      601 attttcgtca atatggcgat gattgatgtg ccgcgcggca atcccgagta tgtgcgtctg
      661 ttgggcatgg cggtgcggct gtaccgtttt atgtcgccgt tgggcttcgg cgcggtcgtg
      721 ttcggcgcgg cgataccgtt tgccgccggc tggtggggca gcggctgggt acacgtcaaa
      781 ctgtgtttgg gcttgatgct cttggcttac cagttgtatt gcggcgtgct gctgcgccgt
      841 tttcaggatt acagcaatgc tttttcacac cgctggtacc gcgtgttcaa cgaaatcccc
      901 gtgctgctga tggttgccgc gctgtatctg gtcgtgttca aaccgttttg acggagcatc
      961 ttatgccgat agaaatcgaa cgccgttttt tgattggaaa cgacaactgg cggcaatacg
     1021 ccgacgaacc gctgctgttg aggcagggtt atctgtctgt tgaaaaagaa cgcaccgtcc
     1081 gtgtccgcat tgccggaaaa caggcgtggc tgacgctgaa aggctatatt tcggaaatca
     1141 gccgcagtga gtttgaatac gaaatcccgc ttgccgatgc ggaaaagatg atggaaacga
     1201 tgtgcccgtt taaaatggaa aaatggcgtt atccggtcag atggggcggc agcctgtttg
     1261 aaatcgatgt ttttcttggc gataatgcgc ctttggtcgt cgcggaaatc gagttgcccg
     1321 acgagaatgc cgattttgac cgtccggact ggctgggacg ggaaatcact gccgacggta
     1381 tgtttaccaa tgcttatttg agcaggcatc cgttctcaag ctggaaaaat gccgtctgaa
     1441 acggcttcag acggcattct ccgacaacca tcgcaaatcc aaagacagcc tctatatcct
     1501 tatttctgct ttctttgttc cattattccc aataaatccc cataacctaa aatctcgtca
     1561 ttcccgcgca ggcgggaatc cggttcttta aacttcggtt ttttccgata aattgcttta
     1621 gcttttcgtt tccggattcc cgcttttgcg ggaatgacga tggaagattg ttgttatttc
     1681 ggataaatta ccctaacttt aaaatcccgg attcccatct tcgggggaat aacaatattt
     1741 tctatttcag cccatcattt gcgggggatt ttatagagag tttatatttc aatatctcaa
     1801 ataatgcaca aaagcatagc tcgtgccttt gctgctgaca cggcgttccg tccgctctgc
     1861 ttctttccaa tgcgtccggt ctattgcggg gaaaaatgca tctccttcca cagacaaatc
     1921 cacttcggtt atccgcaaat cggtcgccaa tggcatcgct tgtccgtata tctgcgcgcc
     1981 gcccataatg acggcttctt ccgcgcctgc gcacaatgcc aatgccgcct ccaaacttgc
     2041 cgccgtttcc gcgcctgccg cgcaataatc cgcctgccgg ctgatgacga tgttcctccg
     2101 tccgggcagg ggtttgacgg gcagggattc ccacgttttc cgccccataa tgacgggttt
     2161 gcccaaggta taggctttga aaaatgcgaa atcttcgggg atgtgccaag gcatagcatt
     2221 gcccgccccg atgcacaggt tttccgcaca cgccgcaatt agggttattt tgagcatatt
     2281 tcctccacaa aaatgccgtc tgaacgcttc agacggcatt ttatgacatt ccgtgtcaaa
     2341 aatctcaacc ggcgcgcgcc atacgttttt tgtttgcacc tgccaacaat gccaacagtt
     2401 cttcagtcgc gccccaaccg atacacgcat cggtaatgct tttgccgtac acttccggct
     2461 tgtcctgcct gccttcgacc aaatggcttt ccaccatcac gcccatgata ttgccgccgt
     2521 cctgttccaa ttgggcggca atatcttgtg ccacttccat ctgccgggtg taatccttgc
     2581 ggctgttggc gtggctgcaa tctatcatca gcttgtcggt tacccctgcc gcacgcagtt
     2641 gttccgccgc ctcgctgacg tgtcccgcat cataattcgg ctcttttccg ccgcgcaaaa
     2701 tgacatgaca gtcaggattg ccgccggtat ggacaatggc ggaatgcccg gccttggtta
     2761 cagacaggaa atgatgcgaa tggctcgccg caccgattgc gtcgatggca atcttcaaat
     2821 tgccgtccgt accgttttta aagccgacgg ggcaggacag cccgcttgcc aattcgcggt
     2881 gaacttggct ttcggtcgtc cgcgcaccga ttgcccccca agaaatcaag tccgcataat
     2941 attgcggcgt aatcatatcc aaaaactcgg tagaggcagg catacccata ttgttcagcg
     3001 acaacaacag gctgcgcgcc tgacgcaaac cgaaattgat gtcaaacgta ccgtccaaat
     3061 gcgggtcgtt aatcaaacct ttccaaccca ccgtcgtcct cggcttctcg aaataaacgc
     3121 gcatcacaat caaaagctcg ttttcatact gcttgcggag tttcaacaaa cgctccgcat
     3181 attccaacgc cgctttcgga tcgtgaatcg aacacggccc gataataacc aacagccgct
     3241 tgtccctgcc gtgaaccaaa tcggaaattt cctgacgggt gcggtgaacc aagcccgaag
     3301 cctctttgga aatcggcagc tcgtaaagat gggcaatcgg cggcaacaac tctttaactt
     3361 ctttaatctt aatatcgtct gtggggtaat ggtgtgtcat cttgcatctt tcctgatggt
     3421 tttttatacg cggcttaggt tacatcgttt accttgcaca aacaagtgca acatattact
     3481 acaaagctat tttatttcaa aataactgat atatcttttt tcatagattt taaatttcat
     3541 atttttaata ttttaggaat tttattccaa agaaaaagaa acagattgat aaaaacaata
     3601 cccgccattc attcggacaa aaccgcacgg aacatccgtt ttcccatttc cacgacaatc
     3661 cggtacagaa caccccgctg ccgattccgc ctgcacatcc ggcaaaattt catacgcaag
     3721 gaaaatgttg aaaacaaaaa gaactaattg cttgaaaaaa gatttaattg gaatgcgggt
     3781 atttcccaca ctttctccat ttattcatcc tcattatagg aagaaaaaca tgaaaaaacc
     3841 ctgcttgcac tctcacttgc agccgtgtcc gccgttacgg tagccgatat ttctgagtct
     3901 aacggcaagg gcaaagccat catcggcgat aaggaaaaac accgttacca agcatgaaaa
     3961 ccttgctttg aaagtccatg gcgaaatccg ccaagatttg ggggacggat gatccctaac
     4021 cccttgggcg gatgcatatg cagatttgcg cggcaaaacc aaagtgatga cgacccagat
     4081 gggtgcttcc cgcgatgtca gcaaaagcgc caaaggttgg agtgtcggta tcggtctgaa
     4141 tgtaggcaaa cagttgaccg acagcgtcga tctcgagttt gatccatact accgtcacaa
     4201 aacaatctgc aaaccccgtg agattgtctt ggacggtgac aaaaccaaaa tgggccgctc
     4261 caaatccaac gagtacggct tccgcgtagc cgcaacgttc tatagtcaat taaaatcaaa
     4321 ataggacagt agtgcatcgt caaatcgggc gtaatcagac aaaacggttc gcagataccg
     4381 cttaatattc gcccacactt tctcaatcgg gttgagct
//
LOCUS       DAXHSF010000153         4389 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.153, whole genome shotgun sequence.
ACCESSION   DAXHSF010000153 DAXHSF010000000
VERSION     DAXHSF010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4389
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.153"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(49..3081)
                     /locus_tag="ACFM8S_001752"
     CDS             complement(49..3081)
                     /locus_tag="ACFM8S_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFC6314792.1"
                     /translation="MFWIVLIVILLLALAGLFFVRAQSEREWMREVSAWQEKKGEKQA
                     ELPEIKDGMPDFPELALMLFHAVKTAVYWLFVGVVRFCRNYLAHESEPDRPVPPASAN
                     RADVPTASDGYSDSGNGTEEAETEEAEAAEEEAADTEDIATAVIDNRRIPFDRSIAEG
                     LMPSESEISPVRPVFKEITLEEATRALNSAALRETKKRYIDAFEKNETAVPKVRVSDT
                     PMEGLQIIGLDDPVLQRTYSRMFDADKEAFSESADYGFEPYFEKQHPSAFSAVKAENA
                     RNAPFRRHAGQAEAKSPDVSQGQSVSDGTAVRDACRRVSVNLKEPNKATVSAEARISR
                     LIPESRTVVGKRDVEMPSETENVFTETVSSVGYGGPVYDEAADIYIEEPAAPDAWVVE
                     PPEVPKVPMPAIDIPPPPPVSEIYNRTYEPPTGFEQAQRSRIAETDHLADDVLNGGWQ
                     EETAAIADDGSEGAAERSNGQYLSETEAFGHDSQAVCPFENVPSERPSCRVSDTEADE
                     GAFPSEETGAVSEHLPTTDLLLPPLFNPEATQTEEELLENSITIEEKLAEFKVKVKVV
                     DSYSGPVITRYEIEPDVGVRGNSVLNLEKDLARSLGVASIRVVETILGKTCMGLELPN
                     PKRQMIRLSEIFNSPEFAESKSKLTLALGQDITGQPVVTDLGKAPHLLVAGTTGSGKS
                     VGVNAMILSMLFKAAPEDVRMIMIDPKMLELSIYEGIPHLLAPVVTDMKLAANALNWC
                     VNEMEKRYRLMSFMGVRNLAGFNQKIAEAAARGEKIGNPFSLTPDDPEPLEKLPFIVV
                     VVDEFADLMMTAGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANIPTRI
                     AFQVSSKIDSRTILDQMGAENLLGQGDMLFLPPGTAYPQRVHGAFASDEEVHRVVEYL
                     KQFGEPDYVDDILSGGGSEELPGIGRSGDGETDPMYDEAVSVVLKTRKASISGVQRTL
                     RIGYNRAARLIDQMEAEGIVSAPEHNGNRTILVPLDNA"
     gene            3246..3926
                     /locus_tag="ACFM8S_001753"
     CDS             3246..3926
                     /locus_tag="ACFM8S_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HFC6314793.1"
                     /translation="MIFQTVWFSDMVLSVSWIVLILILAASAPSAFRSLARYRSALPL
                     CTVIFSAAWCLNASAGGGQLAQMNYHLLAVNLVALMVGTSAAFWLAALLMLPYCLLFA
                     GSAGAYPPNALVLILPALAVNRLSRMLVNRLPPNIFIFIFVNGFLASAAGILLTGLVL
                     TGILDAANAFPSEILWTTALPVFILLAWAEAFLSGISTAIFVALKPHWINTFDDNRYL
                     KSDRGIWR"
     gene            3955..4314
                     /locus_tag="ACFM8S_001754"
     CDS             3955..4314
                     /locus_tag="ACFM8S_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFC6314794.1"
                     /translation="MLSNIIPLSIGAALGATARWLLNLAVPAAMSPATGNLFANWTGA
                     LLIGIFAETINHPQWKLLLITGFLGSLTTLSGFSLETVTLLQSNRPASALANIFLHTA
                     GSLLLTWLGLKIGAAVK"
BASE COUNT          888 a         1286 c         1124 g         1091 t
ORIGIN      
        1 aaatgccgtc tgaaacagtc ttcagacggc atttccattt gcaaaaaatc aagcattgtc
       61 caaggggacg agaatcgtac ggttgccgtt gtgttccggt gcggacacaa tgccttccgc
      121 ctccatctgg tcaatcagac gcgcggcgcg gttgtagccg atgcgcaagg tgcgctgtac
      181 gcccgaaata ctggctttgc gcgttttcag gacaacggat acggcttcat catacatcgg
      241 atcggtttcg ccgtcgccgc tgcgcccgat gccgggcagc tcttcgctgc cgccgccgct
      301 caaaatatcg tcaacatagt ccggttcgcc aaactgcttc agatattcga ccacgcggtg
      361 cacctcttca tccgaggcaa acgcgccgtg aacgcgctgc ggataggccg tacccggcgg
      421 caggaacagc atatcgccct gaccgagcag gttttccgcg cccatttggt cgagaatcgt
      481 gcggctgtcg attttgctgg acacttggaa cgcgatacgc gtcgggatgt tcgccttaat
      541 cagacccgtg atgacatcga cgctggggcg ttgtgtggca aggataagat ggatgcctgc
      601 cgcgcgggct ttttgggcga ggcgggcaat cagttcctcg attttcttgc ctgccgtcat
      661 catcaaatcg gcaaactcat cgaccaccac cacgataaac ggcagttttt ccaaaggttc
      721 gggatcgtcg ggcgtgaggc tgaacgggtt gccgattttc tccccccttg ctgcggcttc
      781 ggcgattttt tgattgaaac ccgcaagatt gcgcacgccc ataaagctca tcaggcggta
      841 gcgtttttcc atttcgttaa cacaccagtt cagcgcgttt gccgccagct tcatatcggt
      901 aacgacaggg gcgagcaggt gcgggatgcc ttcgtaaatg ctcaattcca gcattttcgg
      961 atcgatcata atcatacgca cgtcttccgg cgcggctttg aaaagcatag acagaatcat
     1021 cgcgttgaca cccaccgatt tgcccgaacc ggtcgtgccg gcaaccagca aatgcggtgc
     1081 tttgcccaag tcggttacga cgggctgtcc ggtgatgtcc tgaccgagcg cgagcgtcag
     1141 cttggatttg gattcggcaa actcgggcga attgaagatt tcgctcaggc gtatcatttg
     1201 gcgtttcggg ttcggaagtt ccaaacccat acaggttttg ccgaggatgg tttcgacaac
     1261 gcggatggaa gccacgccga gcgaacgcgc caagtctttt tccagattta gaacggaatt
     1321 gccgcgcacg ccgacatcgg gttcgatttc ataacgcgta atcacggggc cggaataaga
     1381 atcgacaacc ttgaccttga ctttgaactc cgccaatttt tcttcgatgg tgatgctgtt
     1441 ttccaacagt tcttcttcgg tttgcgtcgc ctcgggattg aacagcggcg gcagaagcag
     1501 gtcggttgtc ggcaggtgtt cggataccgc accggtttct tcagatggga acgccccttc
     1561 atccgcttcc gtatccgata cccggcagga cgggcgttca gacggcacat tttcaaacgg
     1621 acaaaccgcc tgactgtcat gcccgaacgc ttcggtttcc gacagatatt gcccgtttga
     1681 ccgctctgcc gcaccttcac tgccgtcatc tgcaatagcg gcggtttcct cctgccaacc
     1741 tccattcaaa acatcatcgg caagatggtc ggtttcagca atgcggctgc gttgcgcctg
     1801 ctcgaatcct gtcggcggct cataggtacg gttgtagatt tccgatacgg gaggcggcgg
     1861 cggaatatcg attgcgggca tgggaacttt cggcacttcg ggtggttcga ccacccaagc
     1921 atcgggcgca gcaggttctt caatatagat atcggcagct tcatcataaa ccggaccgcc
     1981 gtatcccaca gacgaaacgg tttccgtgaa aacattttcg gtttcagacg gcatttcgac
     2041 atcccgtttc ccgacaaccg tccgactttc cggaatcagg cgcgaaatcc gcgcctccgc
     2101 agaaaccgtt gccttgttcg gttctttcaa attgacggaa acgcggcggc aggcatcgcg
     2161 gacggctgtg ccgtctgaaa cggactgtcc ttgggaaaca tccggggatt ttgcctccgc
     2221 ctgccctgca tgacggcgga acggcgcatt ccgtgcattt tcggctttga ctgcagaaaa
     2281 ggcagacgga tgctgcttct caaaatacgg ctcaaatccg taatccgcag actcggaaaa
     2341 cgcttctttg tccgcatcga acatacggga atacgtgcgt tgaagcacag ggtcgtccaa
     2401 accgataatc tgtagccctt ccatcggggt atcggacacg cggactttgg ggaccgctgt
     2461 ttcgtttttc tcaaatgcat cgatatagcg ttttttcgtt tcccttaaag ccgcgctgtt
     2521 taaagcacgc gttgcttctt ccaaagtgat ttccttaaaa accggacgga cgggcgaaat
     2581 ttcgctttca gacggcatca acccttcagc aatactccgg tcgaatggga tgcggcggtt
     2641 gtcgattacg gcagttgcaa tgtcttccgt atcggcagcc tcttcctccg cagcttctgc
     2701 ttcttccgtt tccgcttctt ccgtcccgtt tccactgtct gaatatccgt cggatgcggt
     2761 cggaacatcc gcacggtttg cagaagcagg cggaacgggc ctgtccggtt cggattcgtg
     2821 cgccagatag tttcggcaga aacggacgac accgacaaac agccaataca ctgccgtttt
     2881 gacggcatgg aaaagcatca gggcaagttc gggaaaatcg ggcataccgt ctttgatttc
     2941 aggcagctcc gcctgttttt cccctttctt ttcctgccac gcagaaacct cgcgcatcca
     3001 ctcgcgttcg gattgtgcgc ggacaaaaaa caagccggca agcgcaagca acaaaataac
     3061 gatcaaaact atccaaaaca tatgccgttt cccgcaagac aaacgattaa acgggtttat
     3121 tttaacgtgt ttccatccca aaaaaaacaa ccggtcggca attctgccga aaccgattcg
     3181 gctttcggtt tgcccccgcc gacagtataa tagccgtcct ttcccttaat ctttgcggca
     3241 ttgccatgat tttccaaaca gtctggtttt cagatatggt actgtcggtt tcgtggattg
     3301 tccttatcct gatactggca gcttccgcgc cgtccgcatt ccgctcgctt gcccgctacc
     3361 gttccgccct tcccctgtgt accgtaattt tttccgcagc atggtgtctg aacgcctctg
     3421 ccggcggcgg acagcttgcc cagatgaact accatctgct cgccgtcaat ctggttgcac
     3481 tgatggtggg cacttccgcc gccttttggc ttgccgcgct gctgatgctg ccctactgcc
     3541 tgctgtttgc cggttctgcc ggcgcatatc cgccgaacgc tttggtgctg attctgcccg
     3601 cgcttgccgt caaccgcctg tcgcgtatgc tggtcaaccg cctgccgccc aatattttca
     3661 tctttatttt cgtcaacggc tttctcgctt ccgccgccgg cattctgttg accgggctgg
     3721 tgctgaccgg tattttggat gctgcaaacg cttttccgtc cgaaatattg tggacgaccg
     3781 ccctgcccgt ctttattctg ctggcgtggg cggaagcctt cctcagcggc atttcaactg
     3841 ccatatttgt tgcactgaag ccccattgga tcaacacttt cgacgacaac cgctatctga
     3901 agtccgaccg cggcatatgg cggtagcctt cagacggcat aggcagacaa aaccatgctt
     3961 tccaacatca tccccctttc catcggtgcg gcactcggtg caacggcgcg ttggctgctc
     4021 aacctcgccg ttcctgccgc catgtctccc gccacgggca acctgtttgc caactggacg
     4081 ggcgcgctgc tcatcggcat ctttgccgaa accatcaatc acccgcaatg gaagctgctg
     4141 ctgattaccg gttttctcgg cagcctgacc acgctttccg gattttcact ggaaaccgta
     4201 accctgctcc aatcgaatcg tcctgcttca gcacttgcca atattttcct acacacggca
     4261 ggttcgctgc tgctgacttg gctcgggctg aagatagggg cggcagtcaa ataaccgcaa
     4321 caaattgcct ccctttgttt tgacataatc tataaattag cttcgcttgt ttaaatactg
     4381 gctgtgcca
//
LOCUS       DAXHSF010000154         4339 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.154, whole genome shotgun sequence.
ACCESSION   DAXHSF010000154 DAXHSF010000000
VERSION     DAXHSF010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.154"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(1..1001)
                     /locus_tag="ACFM8S_001755"
                     /pseudo
     CDS             complement(1..1001)
                     /locus_tag="ACFM8S_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222110.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
     gene            complement(1012..1806)
                     /locus_tag="ACFM8S_001756"
     CDS             complement(1012..1806)
                     /locus_tag="ACFM8S_001756"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFC6314795.1"
                     /translation="MKAYLDLMRHVLDNGTDKSDRTGTGTRSVFGYQMRFDLGKGFPL
                     LTTKKLHLRSIIHELLWFLKGDPNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRSWP
                     APDGRHIDQIANVVEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFIHTFGDAHLYRNHFEQAEL
                     QLEREPRTLPVMKINPEVKDLFSFKFEDFELEGYDPHPHIKAAVSV"
     gene            2190..3524
                     /gene="gdhA"
                     /locus_tag="ACFM8S_001757"
     CDS             2190..3524
                     /gene="gdhA"
                     /locus_tag="ACFM8S_001757"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFC6314796.1"
                     /translation="MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQ
                     QSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
                     VPAGDIGVGGREIGYLFGQYKKIRNEFSSVLTGKGLEWGGSLIRPEATGYGCVYFAQA
                     MLQTRNDSFEGKRVLISGSGNVAQYAAEKAIQLGAKVLTVSDSNGFVLFPDSGMTEAQ
                     LAALIELKEVRRERVATYAKEQGLQYFENQKPWGVAAEIALPCATQNELDEEAAKTLL
                     ANGCYVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWT
                     REEVDQRLFGIMQSIHESCLKYGKVGDTVNYVNGANIAGFVKVADAMLAQGF"
     gene            3666..4040
                     /locus_tag="ACFM8S_001758"
     CDS             3666..4040
                     /locus_tag="ACFM8S_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314797.1"
                     /translation="MIMDQYYMELKNKLSNRPILLDNTNDFLFVLVNTVKAMIENTDK
                     SQLSELDKILDGVTSQELKLAYDFCQGKFGQAGFSYRRHPNYFYLSSLIATFPEFELS
                     KADRDYLKGIINFDNYLLYELD"
     gene            4084..>4339
                     /locus_tag="ACFM8S_001759"
     CDS             4084..>4339
                     /locus_tag="ACFM8S_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone acetyltransferase"
                     /protein_id="HFC6314798.1"
                     /translation="MIELKLVDESSFQAVLDLKISEADERARFVAPNVRSLADAWLYR
                     ENEDVFPRAIYWDKQVVGFLLLEIDKDEAEYFIWRIMIGQQ"
BASE COUNT         1157 a         1159 c         1014 g         1009 t
ORIGIN      
        1 ccggcatgac cagccaaacc ttaagccgcc tgctttcggc aaccgaccgt atcaagggca
       61 aatccataat ggaaaacgcc gtcgacaagg catcggcaac cgccgcatca tccgccatca
      121 cgctcatgct tttataacgc ggcgtacccg cgccggtccg cggatcgaac aaatgggtaa
      181 acctgcccgc cttgtccatc accgtcccat agccgcccga agtggcaaac gccttgtctt
      241 tcatcgtgat atttgccaac acgccttctt cgtcgtccag attgcggatg ccgacattcc
      301 aagtccgttt accgtttgta tcaaatcccc tgatttcgcc catatcgacc agcgcggcag
      361 gcacgccgtt tgctttaagc agcgcaacaa ccttgtccgt gatatagcct tgcgcgatgc
      421 cgttcaagga caaacccata cccttttcgg caaaacggat ttcacggtcg tcaaacgaga
      481 ctttgccgaa gccgacccgc ttcagggctt ccttcacgct attctccggc ggcggtgttt
      541 ccgcatcagg atgggcggca aaataatcgg catacagttt ccacaacgcc tgcactgtcg
      601 gatcgaacgc gccgtccgtc agcgcgtgaa tatcgcggca caggctcaac agttccaaaa
      661 aatccgccga cggcgaagtc agataaccgt ccctgttcag gcggctgatc aggctgtctt
      721 cacggtaaag gctgaacaat tttttccaaa cgcgccactt ccgccaaaac cttgttgacc
      781 aaatccgccg cctgtctgtc gtccacaccg aacaggcgca gctccgcgcc ggaacccagc
      841 gcgacacctt tccagaaaaa cacattttca ttgcgttttt catccccgtt tcgtttttca
      901 ccgtcggcgg caaaaggatt cggcaggaaa gaaaccgccg cgcccgccgc cgcaacggcg
      961 gcaaccgtca gaaaacgcct gcgcccgaaa tgcctgtcca tatacccctc ctcagaccga
     1021 cactgccgcc ttgatgtgcg gatgggggtc gtagccttcc aactcgaaat cttcaaactt
     1081 gaaggaaaac aaatctttga cttcaggatt gattttcatc acaggcaagg tgcgcggttc
     1141 gcgttccaat tgcaactctg cctgctcgaa atggttgcgg tacaaatgcg cgtcgccaaa
     1201 cgtatggata aactcaccgg cttccaatcc gcacacttgc gccatcatca tggtcaacaa
     1261 tgcgtagctg gcgatgttga acggtacgcc aaggaaaata tccgcactac gctggtaaag
     1321 ctggcaggac agtttgccgt cggcaacgta aaactgaaac agcgcgtggc agggcggcaa
     1381 tgccatttca tcgaccaaag ccggattcca cgccgacaca atcaggcggc gcgagtccgg
     1441 attcttcttg atttgttcca ccacattggc aatttggtcg atatggcgtc cgtcgggcgc
     1501 gggccaactg cgccattggt agccgtaaac cggtcccaag tcgccgtttt cgtccgccca
     1561 ctcgtcccaa atggaaacat tgttgtcctt cagatatttg atattgggat cgcctttgag
     1621 aaaccaaagc agctcgtgaa taatcgagcg cagatgtagc tttttggtcg tcagcagcgg
     1681 aaaacctttg cccaagtcaa aacgcatctg ataaccgaat acggagcgcg tgcccgtacc
     1741 ggtgcggtct gatttgtccg taccgttgtc gaggacgtgg cgcatcaagt ccaaataggc
     1801 tttcatagca gtctttcatc aaattaaacg gcgcatattg taacatttcc ggataatgcc
     1861 caaaacacgg atacaggcag gcaggattgt tggcaatttc agtccttttc cacagtaaaa
     1921 cccagtggga aaacaaaatt accttgattg gaatcaaaaa atctagttta attacttaga
     1981 ataaaatttc aataatattt ttatttacga aattaattta tgattattga ttaattatcg
     2041 gcaacaaaca tcaaacatcg aaaacatgga aaaaataatg tcaacaattt ttgccaaatc
     2101 gggcttggca tcagaaaaaa ataggtttat attcccacct acaaatttgt tttcccatta
     2161 gtacactatc aaccaaaagg agtatccgaa tgactgacct gaacaccctg tttgccaacc
     2221 tcaaacaacg caatcccaat caggagccgt tccatcaggc ggttgaagaa gtcttcatga
     2281 gtctcgatcc gtttttggca aaaaatccga aatacaccca gcaaagcctg ctggaacgca
     2341 tcgtcgaacc cgaacgcgtc gtcatgttcc gcgtaacctg gcaggacgat aaagggcaag
     2401 tccaagtcaa ccggggctac cgcgtgcaaa tgagttccgc catcggtcct tacaaaggcg
     2461 gcctgcgctt ccatccgacc gtcgatttgg gcgtattgaa attcctcgct tttgaacaag
     2521 tgttcaaaaa cgccttgacc accctgccta tgggcggcgg caaaggcggt tccgacttcg
     2581 accccaaagg caaatccgat gccgaagtaa tgcgcttctg ccaagccttt atgaccgaac
     2641 tctaccgcca catcggcgcg gacaccgatg ttccggccgg cgacatcggc gtaggcgggc
     2701 gcgaaatcgg ctacctgttc ggacaataca aaaaaatccg caacgagttt tcttccgtcc
     2761 tgaccggcaa aggtttggaa tggggcggca gcctcatccg tcccgaagcg accggctacg
     2821 gctgcgtcta tttcgcccaa gcgatgctgc aaacccgcaa cgatagtttt gaaggcaaac
     2881 gcgtcctgat ttccggttcc ggcaacgtgg cgcaatacgc cgccgaaaaa gccatccaac
     2941 tgggcgcgaa agtgctgacc gtttccgact ccaacggctt cgtcctcttc cccgacagcg
     3001 gtatgaccga agcgcaactc gccgccttga tcgaattgaa agaagtccgc cgcgaacgcg
     3061 ttgccaccta cgccaaagag caaggtctgc aatactttga aaaccaaaaa ccgtggggcg
     3121 tcgccgccga aatcgccctg ccctgcgcga cccagaacga attggacgaa gaagccgcca
     3181 aaaccctgtt ggcaaacggc tgctacgtcg ttgccgaagg tgcgaatatg ccgtcgactt
     3241 tgggcgcggt cgagcaattt atcaaagccg gcatcctcta cgccccggga aaagcctcca
     3301 atgccggcgg cgtagcaact tcaggtttgg aaatgagcca aaacgccatc cgcctgtctt
     3361 ggactcgtga agaagtcgac caacgcctgt tcggcatcat gcaaagcatc cacgaatcct
     3421 gtctgaaata cggcaaagtc ggcgacacag taaactacgt caatggtgcg aacattgccg
     3481 gtttcgtcaa agttgccgat gcgatgctgg cgcaaggctt ctaagcaaac gccgccgctc
     3541 cgcaaacaaa atgccgtccg aaccgcaaat gctgttcaga cggcatttca ttatccgccc
     3601 gttcgaatcg ggtgatacta tttgtatcca gaattgttgg atagtttggg agtggagagg
     3661 aagaaatgat tatggaccaa tactatatgg agttaaagaa taagttatct aacagaccaa
     3721 tcctactgga taatactaat gattttcttt ttgttttagt aaatacagtg aaagctatga
     3781 tagaaaatac tgataaaagt cagctttcag agttagataa aattttagac ggtgtcacaa
     3841 gtcaagaatt gaaacttgcc tatgattttt gtcaagggaa attcggtcaa gcaggttttt
     3901 cttatcgtag gcacccgaat tatttttatt tgtccagttt aattgcgact tttccagaat
     3961 ttgagttgtc taaagcagac cgagattacc tcaaaggaat cataaatttt gataactacc
     4021 ttttatatga gttagattag tctttaatca cctcaaaacc ctatttattc aaaaggagaa
     4081 aaaatgattg agctgaaatt agtagatgag agcagttttc aggcagtgct ggatttgaaa
     4141 atatctgaag ctgatgaacg agcacgtttt gtggctccaa atgtacgctc tttagctgac
     4201 gcatggctct accgggaaaa tgaagatgtg tttccgaggg caatctattg ggataagcaa
     4261 gtggttggct ttctcctgct ggaaatagac aaggatgaag cagaatactt tatctggcgg
     4321 ataatgattg gtcagcagt
//
LOCUS       DAXHSF010000155         4303 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.155, whole genome shotgun sequence.
ACCESSION   DAXHSF010000155 DAXHSF010000000
VERSION     DAXHSF010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.155"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(62..1120)
                     /gene="dinB"
                     /locus_tag="ACFM8S_001760"
     CDS             complement(62..1120)
                     /gene="dinB"
                     /locus_tag="ACFM8S_001760"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFC6314799.1"
                     /translation="MSSRKIIHIDMDAFYASVELREQPHLKGRPVVVAWEGARSVICA
                     ASYEARQFGLHSAMSVATAKRLCPQAVYVPPHFDLYRQVSAQIHAVFRRYTDLIEPLS
                     LDEAYLDVTRNFKNIPYASEVAKEIRAAIFAETGLTASAGIAPNKFLAKIASDWRKPN
                     GQFVLPPHKVMAFLETLPLGKIPGVGKVTLKKMQSLGMRTAGDLRRFERGELLNHFGR
                     YGYRLYDLVRGTDERPVKAERERLQISTEITLPEDLPLEQAAGHLPHLAEDLWRQITR
                     KNVEAQSVTLKLKTYDFRIITRTLTYSSVLPDCAALLQAAQMLMARVPPQTEDAFRLI
                     GIGVGHLVPKNQQQDLWA"
     gene            1354..2133
                     /locus_tag="ACFM8S_001761"
     CDS             1354..2133
                     /locus_tag="ACFM8S_001761"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HFC6314800.1"
                     /translation="MAASPEAKFTEEKILWVKHHTPKLITFAISRPESYRFKAGQFSR
                     LGFYEGEGFIWRAYSIVSAEYADTLEYFAVLIQDGPMSARFAQMQQGDTILLDKNATG
                     FLLPERFPDGKDLVMLCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDR
                     LAALSEHPLVGEYRHSFHFVPVTTRAANPSGLSGKRIPELLKNSSIEQALHTKFTPES
                     TRFMICGNPEMVKDTFQTLLDMGYAMHRNRIPGQIMMENGF"
     gene            complement(2338..3723)
                     /locus_tag="ACFM8S_001762"
     CDS             complement(2338..3723)
                     /locus_tag="ACFM8S_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFC6314801.1"
                     /translation="MIVASRWPLLEKVFLRFGMPVAVVDLESTGGNLYEDRVTEVALV
                     KFEQGRVVRHEWLVNPQKPIPQFVAGLTGISDGMVADAPVFAEIAGELFSVLKGCVLV
                     AHNSRFDYTFLKHEFHRAGIGFSSPALCSVQLSRCLYPQFYKHSLDSIIERLGIVVED
                     RHRAMADVSALCDYLEYSLSEHGVEEWIRQCFRLMNPKPLPAALPERLREQLYGLPDG
                     MGVLACFDGGGKVNYIGTFERVYSEVSALLDSGKAPFDWCNTEEVRFFPALGRLHAHK
                     VKAELVRHYGVDCYVSAKNLLKTFTTVRFEKGSDGILNAKTADLKNGVTDNPPTGLFA
                     NKKAARRALSSWAETYGLCPAAAGILPDGYAEDEPCPVYVSGRCDKACGRSDEQVLAF
                     AHKLPVLDWGKMHEVEITETDPLTGEKVVLHGMGGALEMDDGLWYFDKDLPDAFKAKF
                     KTDRKNIKEIG"
     gene            3841..3917
                     /locus_tag="ACFM8S_001763"
     tRNA            3841..3917
                     /locus_tag="ACFM8S_001763"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3875..3877,aa:Arg,seq:acg)
     gene            3935..4009
                     /locus_tag="ACFM8S_001764"
     tRNA            3935..4009
                     /locus_tag="ACFM8S_001764"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3968..3970,aa:Glu,seq:ttc)
BASE COUNT         1102 a         1278 c          938 g          985 t
ORIGIN      
        1 agccatgccg tctgaaaacc gttcagacgg catatttttt ggacggcgcg cgtaaagcgg
       61 tttacgccca caaatcctgc tgctggtttt tcggcacaag atgccccacg ccgataccga
      121 taaggcggaa cgcgtcttcc gtctgcggcg ggacgcgcgc catcaacatt tgcgcagcct
      181 gcagcagagc tgcgcagtcg ggcaatacgg aggaataagt cagtgtgcgc gtgatgatgc
      241 ggaaatcgta ggtcttcagc ttgagcgtta cgctttgggc ttcgacgttt ttgcgcgtga
      301 tttgccgcca caagtcttcg gcaagatggg ggaggtgtcc ggcagcctgc tcgagcggca
      361 ggtcttcggg cagggtaatt tctgtggaga tttggaggcg ttcgcgttcg gctttgacgg
      421 ggcgttcgtc cgtaccgcgc accaaatcat agaggcggta tccgtagcgt ccgaaatggt
      481 ttaagagttc gccgcgctcg aaacggcgca agtcgcccgc cgtccgcata cccagcgact
      541 gcattttttt cagcgttacc ttgcccacgc cggggatttt gcccaaaggc agggtttcca
      601 aaaatgccat gactttgtgc ggcggcaaca caaactgccc gttcggcttg cgccagtccg
      661 acgcgatttt cgccagaaat ttgttcggcg cgatgcctgc ggatgcagtc aaacctgttt
      721 ccgcaaaaat ggcggcacgg atttctttgg caacttcact ggcgtaaggg atgtttttga
      781 aattacgggt aacgtcaaga taggcttcgt ccagcgacaa gggttcgatt aaatcggtat
      841 aacgcctgaa tacggcgtga atctgcgcgg aaacctgacg gtacaaatcg aaatgcggcg
      901 gcacatacac cgcttgcgga cacagccttt tcgccgttgc caccgacatc gcggaatgca
      961 gcccgaactg ccgtgcctca tacgatgcgg cgcaaatcac cgaacgcgcg ccctcccacg
     1021 cgacgaccac cggccgccct ttcaaatgcg gctgttcgcg cagttctacc gatgcgtaga
     1081 acgcgtccat gtcgatgtgg atgattttgc gtgaagacat cggctctgct gaggataaaa
     1141 gggatattct actgccggca tcgggcaaat tccaaatata cgccccgata tgggaagccc
     1201 cgcatccgca gcgttggaaa caggcaaacg cctgcaaaca aagccggcaa acggataacg
     1261 gcatattgcc tgattgtcat tcagacatag gaaatgcgga aaaaaacacc caaattggct
     1321 ataatcccga caaacacact caaggacaac aacatggcag cctcgcccga agcaaaattc
     1381 actgaagaaa agattttgtg ggtcaaacac cacacgccga aactcatcac tttcgccatc
     1441 agccgtcccg aatcctaccg ttttaaagcc ggacagttct cccgactcgg tttctacgaa
     1501 ggggaaggct ttatttggcg tgcctactcc atcgtttccg cagaatatgc cgacacgctc
     1561 gaatattttg ccgtactcat ccaagacggc cccatgtcgg cccgtttcgc acaaatgcaa
     1621 cagggcgaca ccatcctgct cgataaaaat gccaccggct tcctcctgcc cgaacgcttc
     1681 cccgacggca aggatttggt gatgctctgc accggctccg gcatcgcccc cttcctttcc
     1741 attctcgaac aacccgaaat ccggcagcgt ttcgataccg tcaacctgat acattccgta
     1801 tcttttcccg aagaattgat tttcaacgac cggctcgccg cattgtccga acatcccctg
     1861 gtaggcgaat acagacactc gttccatttc gtccctgtta ccacccgtgc cgccaacccc
     1921 tcgggcttaa gcggaaaacg cattccggaa ctcttaaaaa acagcagcat cgaacaggcg
     1981 ctgcatacca agttcacccc ggaatccaca cggtttatga tttgcggcaa cccggaaatg
     2041 gtcaaagata ctttccaaac gctgcttgac atgggttacg ccatgcaccg caaccgcatt
     2101 cccggtcaaa tcatgatgga aaacggcttc taaaaaccac cctgcttgtc cgatgccttc
     2161 ggatggacgg tcaaaccgac acggcacgaa aaccgcgtcg gcaaaaatgc cgtctgaagc
     2221 cttcagacgg cattttcgga tacatacaaa cggcaacata ttccaaccca aaccgattag
     2281 gcaatcaata cgaacggctg tttacatact taccggcttt caccaaccga tatcgattta
     2341 accgatttcc ttaatatttt tcctgtccgt tttaaacttc gccttaaacg catccggtaa
     2401 atctttatcg aaataccaaa gcccgtcatc catttccaat gcgccgccca ttccatgcag
     2461 aacaactttt tcccccgtta aaggatcggt ttcggtaatt tccacctcat gcatttttcc
     2521 ccaatccaaa acaggcaact tgtgtgcaaa cgccaaaacc tgttcgtcag aacggccgca
     2581 cgccttatcg catcggcctg aaacatatac gggacaaggc tcatcctctg cataaccgtc
     2641 cggcaggata ccggctgcgg caggacacaa tccgtatgtt tccgcccacg acgacaaagc
     2701 ccgtctcgcc gcctttttat tggcaaataa tccggtaggc ggattatccg tcacaccgtt
     2761 ttttaaatct gctgttttcg cattcaatat gccgtctgaa cctttttcaa acctgacggt
     2821 cgtaaatgtt ttaagcagat ttttggcaga tacgtaacaa tccaccccat aatgcctgac
     2881 caattccgct ttaaccttat gggcatgcag cctgcccaat gcgggaaaaa aacggacttc
     2941 ctccgtattg caccaatcaa acggggcttt tccggagtcc aataaagccg aaacctcgct
     3001 atatacccgt tcaaacgtac cgatgtaatt tactttccct ccgccgtcga aacaggccag
     3061 cacccccata ccgtcaggca aaccgtacaa ctgttccctc aaccgttcgg gcagcgcggc
     3121 aggcaacggt ttcggattca tcaaacggaa acactgcctg atccactcct cgaccccgtg
     3181 ttccgacagg ctgtattcca aataatcaca caacgccgac acatccgcca tcgcacgatg
     3241 cctgtcttcc acaacaatcc ccaacctttc gatgatactg tccaggctgt gcttgtaaaa
     3301 ttgcggatac agacaccggg acagctgcac actgcacaaa gcaggcgatg aaaatccgat
     3361 acccgcacga tgaaactcat gctttaaaaa cgtatagtcg aaacggctgt tatgtgcaac
     3421 cagcacacaa cccttcaata ccgaaaacaa ctcgccggca atctctgcaa aaacaggcgc
     3481 atcggcaacc atgccgtctg aaatccccgt cagccccgcc acaaactgcg gaatcggttt
     3541 ttgaggatta accaaccact catgcctcac cacccttccc tgctcaaact tgaccaaagc
     3601 cacttcggtt accctgtctt catacagatt gccgcccgtc gattccaaat cgaccacggc
     3661 aaccggcatt ccaaaccgta aaaatacctt ttccagcaag ggccagcggg aagcaacaat
     3721 cattttattc tctttaaatt caaacaacaa accgatattt tacactttta aggcatttca
     3781 tccaacaaaa caattgacag aatccgatga ttaccctaaa attcaaatct ttcttgcagc
     3841 gcacccgtag ctcagttgga tagagtatct ggctacgaac cagagggtcg ggcgttcgaa
     3901 tcgctccggg tgcgccagta agaaaataca atatgcgccc atcgtctagc ggttaggaca
     3961 tcgccctttc acggcggtaa ccggggttcg attccccgtg ggcgtgccaa attccaaatc
     4021 cccgagatta tccctcgggg attttttatt gtctcggcaa ctcgttacca tatctttacc
     4081 tacccccttc atcagaatct cagacacaat cgaatcatat tcaaaccttt gccgtgcaaa
     4141 ccgatatccc ataaccagat gcggtgtccg tccaacattt tacccgattg aaacgcctga
     4201 tatattgcac cccatcaacg tggcattact tttcttaaca atcccctttg acagcaactg
     4261 actagggctt ttttatgcca tcatcaaatt tatagtggat taa
//
LOCUS       DAXHSF010000156         4301 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.156, whole genome shotgun sequence.
ACCESSION   DAXHSF010000156 DAXHSF010000000
VERSION     DAXHSF010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.156"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            155..1054
                     /locus_tag="ACFM8S_001765"
     CDS             155..1054
                     /locus_tag="ACFM8S_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFC6314802.1"
                     /translation="MGKGGDIVVGNGKKYAAPLLVLGCVVFGLGSLIVRSVPVGSYAI
                     AFWRLLISVFVFWFLARFFGQKFPKNRKTVRYALTAGVFLAFDLALWHESIHAVGPGI
                     STLLNSLQIFFLSAIGVFFFGERLSGLKKAGLISAVAGVAMIAGAEFGYNGNAVWGFA
                     SGLVSGLMLALSMVFVRKTHEIEPVALFPSMMILSLGGAVSLVVPALLMDGGVLYPTT
                     WKDAGLVLVYGVVMQCFAWAMVAYAIPLLSLSLTGLLLLSEPVAALFIDYFGLGKPIE
                     GVQWAGVALTLSAIYLGSLKQQS"
     gene            complement(1115..1221)
                     /locus_tag="ACFM8S_001766"
                     /pseudo
     CDS             complement(1115..1221)
                     /locus_tag="ACFM8S_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(1319..2338)
                     /gene="lepB"
                     /locus_tag="ACFM8S_001767"
     CDS             complement(1319..2338)
                     /gene="lepB"
                     /locus_tag="ACFM8S_001767"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFC6314803.1"
                     /translation="MNTMLMSGAAAALLAGIILYFKSDKKRQENGEWSSGLEYAYILT
                     AVGVFAALSLFMSFTAVFLIFVVLCGTAWGVYKYRLKTHPEISESSHFGDYFGSFFPT
                     VLVLFLIRSFIAEPFQIPSSSMRPGLIKGDFILVGKFSYGLRVPVLNNVFIPTGKIER
                     GDVVVFNYPLQPEMTYIKRIVGIPGDVVEYRDKILTVNGKPTSDIPNGTYRYPDDTDP
                     SEIHNTDMFRSGLDGKSFNILKKEGQPAVSLPVLGKYTSDIMSENGYSIEQSGLEHCQ
                     YADDSSGFVCKVPEGRYFAMGDNRDNSADSRYWGFVDDKLVVGKAMFILMNFGDFGRA
                     GTAIR"
     gene            complement(2481..4274)
                     /gene="lepA"
                     /locus_tag="ACFM8S_001768"
     CDS             complement(2481..4274)
                     /gene="lepA"
                     /locus_tag="ACFM8S_001768"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFC6314804.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRFIQYCGGLDLREMSTQVLDSM
                     DIEKERGITIKAQTAALNYKARDGQVYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIDLGVEVVPVLNKIDLPAADPERVEQEIEDIIGIDAVGAVQ
                     CSAKSGIGVEDVLEEIVAKIPAPTGDENAPLQAVIVDSWFDNYVGVVMLIRVKNGTIK
                     LKDKVRFMSTKAETQVEQLGVFTPKSVQKQELKAGEVGFLITGVKELGQAKVGDTVTL
                     VANPATEPLPGFQEVQSQVFAGLYPVESHDYEALRDALEKLQLNDASLKFEPEVSQAL
                     GFGFRCGFLGLLHLEIVQERLEREFDMDLITTAPTVVYEVVLKNGEKIEVENPSKLPD
                     IGSIETILEPIITATILVPQEYVGNVMTLCNQKRGVQVNMQYMGRQVMLTYDLPMNEV
                     VMDFFDKLKSTSRGYASLDYHFKEFQPSDLIKLDIMVNGEKVDALSLIVHRQSAVHRG
                     RELASKMRELIPRQMFDIAVQAAIGSQIIARENVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKRRMKQVGNVEIPQSAFLAILQVSDK"
BASE COUNT          928 a         1075 c         1169 g         1129 t
ORIGIN      
        1 tcgggtttta tctggtagga cggttttttg ccccaacgga aaatagtcgg cctgcccgta
       61 aaatcagccg tttgtccggg tgcagccggg gctttgggct tcagacggca tattttcgga
      121 atggcggcat tcttgccgtc ggcgcggcag ccgtatgggg aagggagggg atattgtggt
      181 cggtaacggc aaaaaatatg ccgcaccatt gctggtgctg ggttgcgtgg tgttcggtct
      241 gggcagtctg attgtcagat ccgtccccgt cggttcgtat gcaatcgcat tttggcggtt
      301 gctgatttcg gtgttcgtat tttggttttt agcacggttt ttcgggcaaa aattcccaaa
      361 aaacaggaaa accgtccgat atgccctgac ggcgggcgtg tttctcgctt tcgatttggc
      421 gttgtggcac gaaagcatac acgcggtcgg gccgggtatt tccaccctgc tcaacagcct
      481 gcaaatcttt ttcttgtcgg caatcggtgt tttctttttc ggcgagcgtt tgagcgggct
      541 gaaaaaggca ggcttaatat cggcagttgc cggcgtggcg atgattgccg gtgcggaatt
      601 cggctacaac ggtaatgcgg tttggggatt cgccagcggt ttggtatcgg gactgatgct
      661 cgccctgtcg atggtgttcg tccgcaaaac ccatgaaatc gagccggtgg cgcttttccc
      721 ttcaatgatg attttgagtt tgggcggcgc ggtatcgctg gttgttccgg cattgctgat
      781 ggatggcggc gtgctttatc cgacgacttg gaaagatgcg ggtttggtgc ttgtgtacgg
      841 cgtggtgatg cagtgcttcg cgtgggcgat ggttgcctat gcgattccgc tgctttcgct
      901 gtcgctgacg gggctgctgc ttttgtccga accggttgcc gccctgttca tcgattattt
      961 cgggttgggc aaaccgattg aaggcgtgca gtgggcaggg gtggcgctga cgctttcggc
     1021 aatttacctc ggttcgctga aacagcagtc ttaatattga ttccatcaag gcagtattgg
     1081 cagttttccg tgcaaaacct gcgttaccgg actgatatag tgaattaaat ttaaaccggt
     1141 acagcgttgg ctcgccttgc cgtactattt gtactgtctg cggctcgccg ccttgtcctg
     1201 atttaaattt aattcactat aggtaggaaa atccgacaac gttagatccg cctgcaaaag
     1261 tgaggaatag caaatgccgt ctgaaactat tttcagacgg cattcttggc ttcctggcct
     1321 aacggattgc cgtaccggcc ctgccgaaat cgccgaagtt catcaaaatg aacattgcct
     1381 tgccgacaac cagcttgtca tccacaaatc cccagtagcg cgaatcggca ctgttgtcgc
     1441 ggttgtcgcc catagcgaaa tagcgtcctt cgggaacttt gcacacgaaa ccgctgctgt
     1501 cgtcggcata ttggcagtgt tccaaaccgc tttgctctat ggaatatccg ttttcagaca
     1561 taatatcgga ggtatatttg cccaatacag gcagggaaac ggcaggctgt ccttcttttt
     1621 tcagaatatt gaaggatttg ccgtctagac cgctgcggaa catatccgtg ttgtggattt
     1681 cggaagggtc ggtgtcgtcg ggataacggt atgtgccgtt aggaatatcg gaagtgggtt
     1741 tgccatttac cgtcaaaatc ttatcccgat attcgaccac atcgcccgga atgccgacaa
     1801 tacgcttgat gtaggtcatc tccggttgca aaggataatt aaaaacgacc acatcgcccc
     1861 gttcgatttt gcccgtgggg atgaatacat tgtttaaaac aggtacgcgc aggccgtagg
     1921 aaaatttgcc gaccaaaatg aaatcgccct tgatcaggcc cgggcgcatt gagctggacg
     1981 ggatttggaa cggttcggcg ataaacgacc ggatgaggaa caataccaaa acggtaggga
     2041 agaaactgcc gaaataatcg ccgaagtggc tgctttccga gatttcggga tgagtcttca
     2101 ggcggtattt atataccccc caagccgtac cgcacaatac aacgaaaatc aggaaaacgg
     2161 cggtaaagct cataaacagg gacaaagcgg caaacacgcc gaccgctgtc aggatatagg
     2221 cgtattcgag gccggaactc cattcaccgt tttcctgccg ctttttgtcg cttttgaaat
     2281 aaaggatgat gccggcaagc agcgcggcag ccgcgcccga cattagcata gtgttcattg
     2341 ttattcctta atgcttaaaa aaccgcccgt ccgtgcaacc gttttaaggc ggcaaattgc
     2401 aaaatttgtt tgcgggcgcg tgcccctgaa atcagggcgg tttgaggggt gttcccgacg
     2461 cgccgccctg tgtgccagag ttatttgtcg ctcacctgca aaatcgccaa gaacgcgctt
     2521 tgcggaattt ccacattgcc cacttgtttc atacggcgtt tacctgcctt ttgtttttca
     2581 agcagttttt tcttacgcgt aatatcgccg ccgtaacatt tcgccaagac gtttttacgc
     2641 agtgctttga cgttttcgcg ggcgataatc tggctgccga tggcggcttg gacggcaatg
     2701 tcgaacattt ggcgcggaat cagctcgcgc attttcgatg ccagctcgcg gcctcggtga
     2761 accgcgcttt gacggtggac aatcaggctc agcgcatcga ctttttcgcc gttgaccata
     2821 atatccagct taatcaaatc agacggttgg aactctttga aatggtagtc caacgaagca
     2881 taaccgcgcg aagtggattt gagtttgtca aagaagtcca tgacgacttc gttcatgggc
     2941 aaatcgtaag tcagcatcac ttggcggccc atgtactgca tattgacctg aacgccgcgc
     3001 ttttggttac acaaagtcat gacgttgccg acgtattcct gcggcacaag gatggtcgcg
     3061 gtaataatcg gctcgagtat ggtttcgatg ctgccgatgt cgggcagttt ggacgggttt
     3121 tcaacttcga ttttctcacc gtttttcaac acgacttcat aaaccaccgt cggcgcggtg
     3181 gtaatcaaat ccatatcgaa ctcgcgctcc aagcgttcct gcacgatttc caagtgcaac
     3241 agacccaaga agccgcaacg gaagccgaag cccaatgcct gggaaacttc aggctcgaat
     3301 ttcaacgaag cgtcgttaag ctgcaatttt tccaaagcgt cgcgcaaggc ttcgtagtcg
     3361 tggctttcca cgggataaag tccggcgaat acttggcttt gtacttcttg gaagccgggc
     3421 agcggctcag tggcagggtt ggcaaccagt gtaaccgtat cgccaacttt cgcctgaccc
     3481 aattctttta cgccggtaat caaaaagccc acttcgccgg ctttgagttc ttgtttttga
     3541 actgattttg gtgtgaatac gcccagctgc tcgacctgcg tttccgcctt ggtgctcata
     3601 aagcgcactt tgtctttcag tttgatggtg ccgtttttca ctcgaatcag catgaccacg
     3661 ccgacgtagt tgtcaaacca cgaatcgaca atcacggctt gcagcggcgc gttttcatcg
     3721 ccggtcggcg caggaatttt ggcaacgatt tcttccaaaa cgtcttccac gccgatgccg
     3781 cttttggcgg aacactgcac cgcgccgacc gcatcgatgc cgatgatgtc ttcaatttcc
     3841 tgctccacgc gttcggggtc ggcggcgggc aggtcgattt tgttcaaaac aggcacaacc
     3901 tccacgccca aatcaatcgc ggtgtaacag ttcgccacgg tttgcgcttc cacgccctgc
     3961 gacgcgtcaa cgaccaaaag cgcgccttcg caggcggaca gcgaacggga aacttcgtaa
     4021 gagaagtcga cgtgtcccgg cgtgtcaatc aggttgagtt gatacacctg cccatcgcgt
     4081 gctttgtagt tgagcgcggc ggtttgcgct ttgatggtaa tgccgcgctc tttttcgatg
     4141 tccatggaat cgagcacctg cgtactcatt tcgcgcaaat ccaagccgcc gcaatattgg
     4201 atgaagcggt cggcaagcgt cgatttgccg tggtcgatat gggcaatgat ggagaaattt
     4261 cggatatttt tcattggatt gtttttaatg ctgaacggta g
//
LOCUS       DAXHSF010000157         4288 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.157, whole genome shotgun sequence.
ACCESSION   DAXHSF010000157 DAXHSF010000000
VERSION     DAXHSF010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.157"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..197
                     /locus_tag="ACFM8S_001769"
     CDS             <1..197
                     /locus_tag="ACFM8S_001769"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314805.1"
                     /translation="LIFVNPLYNQYIKSLLALLVLSMPSNTVRCNEPFSGSMVGNIFD
                     ISKIKSSSINIPSLYWDETL"
     gene            565..2292
                     /gene="ilvB"
                     /locus_tag="ACFM8S_001770"
     CDS             565..2292
                     /gene="ilvB"
                     /locus_tag="ACFM8S_001770"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HFC6314806.1"
                     /translation="MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKH
                     ILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQV
                     GNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELAETIKKAFQIAASGRPGPVVVDV
                     PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLG
                     NASEELTRFVRMTGAPCTGTLMGLGAYPSSDRQFLGMLGMHGTYEANLAMQNADVVLA
                     VGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKVDIPIVGDVKNVLSEMVALW
                     QKQESVPSEDALGKWWKTIEEWRSQDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITS
                     DVGQHQMFAAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSI
                     QMNIQELSTCFQYRIPVNVITLNNGYLGMVRQWQEIYYGGRESETYFDSLPDFVKLAE
                     AYGHIGIRVDKKSDVEGALLEALNQKDRLVFIDFLTDQKQNVMPMVGNGKGLDEMVLP
                     PHMRADGKA"
     gene            2303..2794
                     /gene="ilvN"
                     /locus_tag="ACFM8S_001771"
     CDS             2303..2794
                     /gene="ilvN"
                     /locus_tag="ACFM8S_001771"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFC6314807.1"
                     /translation="MRHILSVLIENESGAMSRVVGLFSARDYNIDSLAVAPTEDKTLS
                     RMTIVTHGDEQVIEQITKQLNKLIEVIKVVDLNESRFVERELMLVKVRAAGKDRDEFL
                     RLTEIYRGSIIDVTDRSYTIEITGSTDKLDSFLETVGRAQILETVRTGAAGIGRGERI
                     LKI"
     gene            2861..3154
                     /locus_tag="ACFM8S_001772"
     CDS             2861..3154
                     /locus_tag="ACFM8S_001772"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFC6314808.1"
                     /translation="MSNIKIVALVTVKPEYTETLAAQFKELVKASRAEEGNISYDLHQ
                     EIGKPNRFVFVENWKSQAAIDEHNASAHFQAFVQSVDGKTEALEIVLMNEVAD"
     gene            3234..4247
                     /gene="ilvC"
                     /locus_tag="ACFM8S_001773"
     CDS             3234..4247
                     /gene="ilvC"
                     /locus_tag="ACFM8S_001773"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFC6314809.1"
                     /translation="MQVYYDKDADLSLIKGKTVAIIGYGSQGHAHAANLKDSGVNVVI
                     GLRHGSSWKKAEAAGHVVKTVAEATKEADVVMLLLPDETMPAVYHAEVTANLKEGATL
                     AFAHGFNVHYNQIVPRADLDVIMVAPKGPGHTVRSEYKRGGGVPSLIAVYQDNSGKAK
                     DIALSYAAANGGTKGGVIETTFREETETDLFGEQAVLCGGVVELIKAGFETLTEAGYA
                     PEMAYFECLHEMKLIVDLIFEGGIANMNYSISNNAEYGEYVTGPEVVNASSKEAMRNA
                     LKRIQTGEYAKMFIQEGNVNYASMTARRRLNADHQVEKVGAQLRAMMPWITANKLVDQ
                     DKN"
BASE COUNT         1116 a         1028 c         1077 g         1067 t
ORIGIN      
        1 tcctgatttt tgttaatcca ctatacaatc aatatattaa gtcgttgtta gccctcttgg
       61 ttctgtcaat gccgtccaat acagtccggt gcaatgagcc attttcagga agtatggttg
      121 ggaatatttt tgatatatca aaaataaaat caagttcaat caatatcccg agtctttatt
      181 gggatgaaac cctgtaaatt ttacaattct tgatatcgaa gaagggtttt tcacatctgc
      241 ccctcctaca caaactgtca acaatttcca tctttcaaaa aaggtactcc tgattttatt
      301 cttttaaaat caatcaataa tttctctgct ttcaaaaaat attaaaagat gaaagatttt
      361 tcttgattga actgtcggtt tgcgttatcg tttccactct ttttcacaaa ctctgtgttc
      421 ctttccaatt gattggataa tccgtctgtt gccgtgttcc cccaatgcgc gtaccctaaa
      481 tcgctgcttg gtggaattgc attacaggtg ctgtggcaaa gcggtttgtc cctattggtt
      541 tgaaaccgta taaaagaggt cgttatgcag ttatcaggcg cgcaaatcat agtgcagagt
      601 ctcaaagccg aaggtgtcga gtacgttttc ggttatcccg gcggtgcggt tatcgaaatc
      661 tacgatgccc ttttccaact caataaattc aagcacattc tgacccgtca cgagcaggcg
      721 gcagtacacg cggcagatgc gtatgcgcgc gtcagcggta aggtgggcgt ggcattggtt
      781 acatccggcc cgggcgttac caatgcactg accggtattg ctactgccta tacggattcg
      841 attccgatgg tggtcatcag cgggcaggta ggcaattccc tgattggtac ggatgcgttc
      901 caagaagttg atacggtggg tattacccgt ccgtgcgtca aacacaattt cctggttacg
      961 gatatcaatg agttggcgga aaccattaaa aaggcgttcc aaattgccgc aagcggccga
     1021 ccggggcctg tggtggttga tgtcccgaaa gatgttacgc aggcgatggc gaaattcagc
     1081 tatcctcagg aagacatttt tatccgttcg tatcaaccgg ttgttcaagg gcatatcggt
     1141 cagattaaaa aggccgtgca gatgttggca tctgccaaac gtccagtcgt ctattttggc
     1201 ggcggcgtgg tgttgggtaa tgcttctgaa gagctgaccc gatttgtccg aatgacgggt
     1261 gctccgtgca cgggtacgct gatgggcttg ggcgcttatc cttccagcga ccgccaattc
     1321 ctcggtatgc tcggtatgca cggtacttac gaggcaaacc ttgccatgca gaatgcggat
     1381 gtcgttcttg ccgtaggtgc gcgttttgac gaccgtgtgg tttccgtacc gtccaaattt
     1441 ttcgagaagg ctaagaaggt tatccatatc gatgtcgatc cttccagcat cgccaaacgc
     1501 gtgaaggtgg acattccgat tgtcggcgac gtgaaaaacg ttttgtccga gatggttgcg
     1561 ctgtggcaaa aacaagagtc cgtgccgtct gaagatgctt tgggcaaatg gtggaaaacc
     1621 atagaggaat ggcgttccca agattgcttg tggtttgaca acggcagcga aattatcaag
     1681 ccacaatatg tgattcagaa gcttgccgag attaccggca attcggcaat catcacatcg
     1741 gatgtagggc agcatcaaat gtttgcggct caatattatc ccttcgaacg tccgcgccaa
     1801 tggctcaatt ccggcggttt gggtacgatg ggcgtgggtc tgccttatgc gattggtgca
     1861 aaacttgccg ccccggatca agacgtattc tgtattaccg gcgacggctc gatccagatg
     1921 aacatccaag agctgtccac ctgtttccaa tatcggattc cggttaacgt cattacgctg
     1981 aacaacggtt atctcggtat ggtacgccag tggcaggaaa tatattacgg cggacgagag
     2041 tcggaaacct atttcgattc tttgcccgat ttcgtcaaac ttgccgaggc atacggccat
     2101 atcggtatcc gcgtggacaa gaagtctgat gtggaaggtg cgttgttgga agcattgaac
     2161 caaaaagaca ggctggtgtt tatcgacttc ctgaccgacc agaaacagaa tgtgatgccc
     2221 atggtcggca acggcaaagg tttggacgaa atggtacttc cgccgcatat gcgtgcggac
     2281 ggaaaggcgt aaggagaggc aaatgcgaca tatcttatct gttctgatcg aaaacgaatc
     2341 aggtgcgatg agccgcgtgg tcggtttgtt ctctgcacgc gattacaata tcgattcttt
     2401 ggcggttgcg ccgaccgaag acaaaaccct gtcccgtatg accatcgtta cacacggcga
     2461 cgagcaggtc atcgaacaaa tcaccaagca actcaataaa ttgattgaag tgattaaagt
     2521 ggtcgatttg aacgaaagcc gttttgtcga acgcgaactg atgttggtaa aagtccgtgc
     2581 cgccggcaaa gaccgcgacg aatttttacg cttgaccgaa atctaccggg gcagcatcat
     2641 cgacgtaacc gaccgcagct atacgattga aattaccggc tcgaccgaca agctggattc
     2701 ctttttggaa acggtcggac gcgcccaaat tttggaaacc gtacgtacag gcgcggccgg
     2761 catcggtcgc ggtgagcgta ttttgaaaat ttaacgccgt aacctttcag acggcacggt
     2821 atttgaatgt cgtctgaaaa acgaacggca ggagagattt atgtcgaaca ttaaaattgt
     2881 cgcactggtt accgtcaaac cagaatacac agaaacactg gcagcacagt ttaaagaact
     2941 ggtcaaagcc agccgtgcag aagagggcaa catcagctac gatctccatc aggaaatcgg
     3001 caaaccgaac cgttttgttt tcgtggaaaa ttggaaatcc caagcagcta ttgacgaaca
     3061 taacgccagc gcgcatttcc aagccttcgt ccagtccgtc gacggcaaaa ctgaagcgtt
     3121 ggaaatcgta ttgatgaatg aagttgccga ttaagcggca cactctgttt aacccgtccg
     3181 aagccgctca acattttaag gcttcgacaa ccatttacct taaaggaaat caaatgcaag
     3241 tctattacga taaagatgcc gatctgtccc tgatcaaagg caaaaccgtt gccatcatcg
     3301 gttacggttc gcaaggtcat gcccatgccg ccaacctgaa agattcgggt gtaaacgtgg
     3361 tgatcggtct gcgccacggc tcttcttgga aaaaagccga agcagccggc catgtcgtca
     3421 aaaccgttgc tgaagcgacc aaagaagccg atgtcgttat gctgctgctg cctgacgaaa
     3481 ccatgcctgc cgtctatcac gccgaagtta cagccaattt gaaagaaggc gcaacgctgg
     3541 catttgcaca cggcttcaac gtgcactaca accaaatcgt tccgcgtgcc gacttggacg
     3601 tgattatggt tgctcccaaa ggtccgggtc ataccgtacg cagtgaatac aaacgcggcg
     3661 gcggcgtgcc ttctctgatt gccgtttacc aagacaattc cggcaaagcc aaagacatcg
     3721 ccctgtctta tgcggctgcc aacggcggca ccaaaggcgg tgtgattgaa accactttcc
     3781 gcgaagaaac cgaaaccgat ctgtttggcg aacaagccgt attgtgcggc ggcgtggtcg
     3841 agttgatcaa ggcgggtttt gaaaccctga ccgaagccgg ttacgcgcct gaaatggctt
     3901 acttcgaatg tctgcacgaa atgaaactga ttgttgacct gattttcgaa ggcggtattg
     3961 ccaatatgaa ctactccatt tccaacaatg cggagtacgg cgaatacgtt accggccctg
     4021 aagtggtcaa tgcttccagc aaagaagcca tgcgcaatgc cctgaaacgc attcaaaccg
     4081 gcgaatacgc aaaaatgttt atccaagagg gtaatgtcaa ctacgcgtct atgactgccc
     4141 gccgccgtct gaatgccgac caccaagttg aaaaagtcgg cgcacaactg cgtgccatga
     4201 tgccttggat tactgccaac aaattggttg accaagacaa aaactgattg ttttcaaacg
     4261 ggactgccta cacatcgtgt aggcagtt
//
LOCUS       DAXHSF010000158         4217 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.158, whole genome shotgun sequence.
ACCESSION   DAXHSF010000158 DAXHSF010000000
VERSION     DAXHSF010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.158"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(109..1152)
                     /gene="argC"
                     /locus_tag="ACFM8S_001774"
     CDS             complement(109..1152)
                     /gene="argC"
                     /locus_tag="ACFM8S_001774"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFC6314810.1"
                     /translation="MSKKIKVGIVGATGYTGVELLRLLAAHPDVEVAAVTSRSEAGTA
                     VADYFPSLRGVYGLAFQTPDEAGLEQCDIVFFATPNGIAMKDALRLLEQGVRVIDLSA
                     DFRIRDIPTWEHWYGMTHAAPGLVSQAVYGLSELNREAVAQARLVANPGCYPTCVSLP
                     LVPLLRQCRLKPGMPLIADCKSGVSGAGRKGNVGSLLCEAGDNFKAYGIAGHRHLPEI
                     RQTIAGLQDGIAEGFVFTPHLAPMIRGMHATVYLHLSDGICPETILRDYYRDSLFVDI
                     LPAGSTPETRSVRGANLCRISIQQAAQSDVWVVLSVIDNLVKGAAGQAVQNMNIMFGL
                     KETHGLGAIPLLP"
     gene            complement(1149..1430)
                     /locus_tag="ACFM8S_001775"
     CDS             complement(1149..1430)
                     /locus_tag="ACFM8S_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314811.1"
                     /translation="MKTLVLLLLSFSTTTAFAAYGLGLGQAPKYPADFRAYGYVYSGR
                     QGWVLKTEADAIKLDTLFKRFVYRPSAANQNPVGQRSDGIPANHTKEKP"
     gene            1684..2839
                     /locus_tag="ACFM8S_001776"
                     /pseudo
     CDS             1684..2839
                     /locus_tag="ACFM8S_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704893.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            2848..3075
                     /locus_tag="ACFM8S_001777"
     CDS             2848..3075
                     /locus_tag="ACFM8S_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314812.1"
                     /translation="MKNWKQFTFFVILVIACYQVLYFLSDMFLLDYINKYSWNLNFIQ
                     GTLNFFSIYLPYVFVSRIFRNTNQEKEYKND"
     gene            3068..3421
                     /locus_tag="ACFM8S_001778"
     CDS             3068..3421
                     /locus_tag="ACFM8S_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314813.1"
                     /translation="MIELQLHELKLVSGGGPVTDNIAGNVANAATTKGGPTWGDLVAI
                     PAAAAAVYYLPKNAYGAAGANGVYNVTRNWVNDAVNAPPYNGRPIFEIEHGLTAPATK
                     ADKSGNGYTDGTDYC"
     gene            3482..4108
                     /locus_tag="ACFM8S_001779"
     CDS             3482..4108
                     /locus_tag="ACFM8S_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein"
                     /protein_id="HFC6314814.1"
                     /translation="MNRPKQPFFRPEVAVARQTSLTGKVILTRPLSFSLWTTFASISA
                     LLIILFLIFGNYTRKTTVEGQILPASGVIRVYAPDTGTITAKFVEDGEKVKAGDKLFA
                     LSTSRFGAGGSVQQQLKTEAVLKKTLAEQELGRLKLIHENETRSLKATVERLENQKLH
                     ILQQIDGQKRRIRLAEDQWQKRHWADRNCRDWAWYPPIWRRATNLFIS"
     gene            4012..4217
                     /locus_tag="ACFM8S_001780"
                     /pseudo
     CDS             4012..4217
                     /locus_tag="ACFM8S_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1128 a          958 c         1091 g         1040 t
ORIGIN      
        1 tttcgtggga atggatgggc ccaaagccgt ccgaaccaaa aaaaaccgcc gctttcagac
       61 ggcatcaaaa aaccgcaggc acgatgcctg cggtttgccg tttgcgcttc aggggagcag
      121 ggggattgcg cccaagccgt gtgtttcctt cagtccgaac atgatgttca tattttgcac
      181 cgcctgaccc gccgcgcctt tgacgaggtt gtcgatgacg gaaaggacga cccacacatc
      241 ggattgcgcc gcctgttgga tgctgatgcg gcagaggttt gcgccgcgca cgctgcgggt
      301 ttcgggtgtc gaaccggcgg gcaggatgtc cacgaacagg ctgtcgcggt aatagtcgcg
      361 caggatggtt tcggggcaga tgccgtctga aaggtggagg taaacggtgg cgtgcatacc
      421 gcgtatcatc ggcgcgaggt gcggcgtgaa cacgaatcct tcggcgatgc cgtcctgaag
      481 cccggcgatg gtctgcctga tttcgggcag gtggcggtgt ccggctatgc cgtaggcttt
      541 gaagttgtcg ccggcttcgc acaacagcga accgacattg cctttcctgc ccgcgccgga
      601 cacgccggat ttgcagtcgg caatcagcgg cataccgggc ttcagacggc attgccgcaa
      661 cagcggcacg agcggtaggg atacgcaggt cgggtagcag ccggggttgg cgacgaggcg
      721 cgcctgtgcg acggcttcgc ggttgagttc gctcaatccg tacacggctt gggaaacgag
      781 gccgggggcg gcgtgggtca tgccgtacca gtgttcccag gtcggaatgt cccgtatgcg
      841 gaagtcggcg gaaaggtcga tgacgcgcac gccctgttcc aaaaggcgca gcgcgtcttt
      901 catagcgatg ccgttgggcg tggcgaagaa gacgatgtcg cattgttcca aacctgcctc
      961 gtcgggcgtt tggaaggcga ggccgtacac gccgcgcaga cttggaaagt aatcggcaac
     1021 tgcggttccc gcttcgctgc ggctggttac ggcggcgact tcgacatcgg gatgggcggc
     1081 aagcaggcgc agcagttcca cgccggtgta gcccgtcgcg ccgacaatgc cgactttgat
     1141 ttttttactc atggtttttc ctttgtgtgg ttggcgggta tgccgtccga acgctgtccg
     1201 acggggtttt gattggcggc ggaaggacgg tacacaaagc gtttgaaaag cgtgtctaat
     1261 ttaatggcat ccgcctctgt ttttaaaacc cagccctgcc gtccggaata aacataaccg
     1321 taggcgcgaa agtcggcagg atatttcggt gcctgcccca aacccaaacc gtatgcggcg
     1381 aaagcggtgg tcgtggagaa ggaaagcagg aggaggacga gggttttcat aaggttccaa
     1441 accgaacggg gcgatgaagc ccgattatag caaaaggtcg tctgaaaacc gttttcagac
     1501 gaccttttat ttaattacaa taaaaatgtt aacaacaaaa aacaaaccgc ttttttccgt
     1561 ttggagacaa atttttaact aaaattgtca acaattcctt gacgcacaca cacaaaaaac
     1621 tttaatattt gtaaatatat gtaacattat aaattttaaa tattttctga gtaaggggaa
     1681 gtaatgagca tctatctcga cttcggcatt agtcctaaaa cgttccgaca gacttaatct
     1741 gtatcaaaag cccaagctct ttaaaggagc ggttcggaat ctcgaagccg catcttggaa
     1801 agatatcaac gagatatacc aacgagcaga cccaaccgca ccgctgtttt atctgcgtaa
     1861 aaaaggtgca attgttccta aagaagaata cgtcgaaagt ttcgacgctt tgggcaaaac
     1921 tcgctaccgt tttattaaat ccgttatcta cgaacatatg aagaatggtg cgtcgttagt
     1981 ctataaccat attaacaacg agccgttttc agatcatatt tcccgtcaag tcgcccgctt
     2041 tgctggcgca catactattg tcagtggata tcttgctttt ggcagcgacg aatcttataa
     2101 aaaccattgg gacacccgcg atgtgtatgc catccagctt ttcggcaaga aacgttggca
     2161 acttactgcc cctgatgtcc ctatgccatt gtatatgcaa cagactaaag atactgatat
     2221 ttccattcct gaacatatcg atatggatat tatccttgaa gcaggtgatg tcctctacat
     2281 cccgcgcggt tggtggcaca gacctatccc gctcggctgt gaaaccttcc acctcgctgt
     2341 cggtaccttt ccgcccaacg gctataatta cctcgagtgg ctaatgaaga aatttcccac
     2401 catagaaagt ctgcgccaca gtttctcaga ctgggagcaa gataggacgc gtatcaacga
     2461 tactgccaca caaattgctg ccatgattgc cgaccccgtc aattatgaag ccttcagtga
     2521 agactttctc ggcaaagaac gtaccgatac cgcttttcat ctcgaacagt tcgcgaatcc
     2581 caacgctact ccgctttcag acgacgtcag gttgagatta aatgccaata atttggatac
     2641 gttggaaaag ggatatttga ttgggaatgg gatgaagata agcgtagatg agttggggaa
     2701 aaaagtgtta gaacacatcg gtaagaatga accgttattg ttgaaaaatc tactggttaa
     2761 cttcaatcag ggaaaatatg aagaagttag gaagttgatt tatcagttga tagagttaga
     2821 ttttctggaa attttgtgag ggattctatg aaaaactgga agcaatttac atttttcgta
     2881 atattagtaa tagcttgtta tcaagtgcta tattttttat cagatatgtt tcttctcgac
     2941 tacattaaca aatatagttg gaatttgaat tttattcagg gtactttgaa ttttttttca
     3001 atatatctgc catatgtttt tgttagccga atttttagga ataccaacca agaaaaggag
     3061 tataaaaatg attgaacttc aacttcatga attgaagctg gtttcagggg gaggtcctgt
     3121 aacagacaat atagctggaa atgtagctaa tgctgccaca accaaaggag gtcccacatg
     3181 gggggatttg gttgcaatac ctgctgccgc agcggcagtt tattatctgc cgaaaaatgc
     3241 ttacggtgct gctggtgcaa atggtgtata caatgtgact cgtaattggg taaatgatgc
     3301 cgttaatgca cctccttata acggaagacc aatctttgag attgaacatg gattaactgc
     3361 tcccgcaaca aaagcagata aatcaggaaa cggctacact gacggtacag attactgctg
     3421 atatttctca tcgtccagac aatctctagg ggtcgtctga aactttctta acttcaattt
     3481 tatgaataga cccaagcaac ccttcttccg tcccgaagtc gccgttgccc gccaaaccag
     3541 cctgacgggt aaagtgattc tgacacgacc gttgtcattt tccctatgga cgacatttgc
     3601 atcgatatct gcgttattga ttatcctgtt tttgatattt ggtaactata cgcgaaagac
     3661 aacagtggag ggacaaattt tacctgcatc gggcgtaatc agggtgtatg caccggatac
     3721 ggggacaatt acagcgaaat tcgtggaaga tggagaaaag gttaaggctg gcgacaagct
     3781 atttgcgctt tcgacctcac gtttcggcgc aggaggtagc gtgcagcagc agttgaaaac
     3841 ggaggcagtt ttgaagaaaa cgttggcaga acaggaactg ggtcgtctga agctgataca
     3901 cgagaatgaa acgcgcagcc ttaaagcaac tgtcgaacgt ttggaaaacc agaaactcca
     3961 tattttgcaa cagatagacg gtcagaaaag gcgcattaga cttgcggaag atcagtggca
     4021 aaaacggcac tgggcagaca ggaattgtcg ggattgggca tggtatcctc cgatttggcg
     4081 aagagcaacg aacctgttta tctcgtgaaa ataaaacccg acaaaccaac catcactgca
     4141 tacggtgagg aaaaaccgct gcaaatcggc atgacgttgg aagcagacat cctacacgag
     4201 aaacggcgat tgtacga
//
LOCUS       DAXHSF010000159         4211 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.159, whole genome shotgun sequence.
ACCESSION   DAXHSF010000159 DAXHSF010000000
VERSION     DAXHSF010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.159"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(81..1127)
                     /gene="recA"
                     /locus_tag="ACFM8S_001781"
     CDS             complement(81..1127)
                     /gene="recA"
                     /locus_tag="ACFM8S_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFC6314815.1"
                     /translation="MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGS
                     LGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAVAQCQKNGGVCAFVDAEHAFDPV
                     YARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGIDMVVVDSVAALVPKAEIEGDMG
                     DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFY
                     SSVRLDIRRTGSIKKGEEVLGNETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELID
                     IGVKNDIINKSGAWYSYNGAKIGQGKDNVRVWLKENPEIADEIDAKIRALNGVEMHIT
                     EGTQDETDGERPEE"
     gene            1381..2145
                     /gene="aroD"
                     /locus_tag="ACFM8S_001782"
     CDS             1381..2145
                     /gene="aroD"
                     /locus_tag="ACFM8S_001782"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFC6314816.1"
                     /translation="MCSCLVVKNTVIGSGRTKIAVPLVARDAAVLSAVLDQIKNLPFD
                     IVEFRADFLECAGSIGEVLRHTQTVRDALPDKPLLFTFRRHGEGGSFPCSDDYYFKLL
                     DALIESRLPDIIDIELFSGETAVRCAVANAQKNGIAALLCNHEFHRTPPQEEIVCRLK
                     QMEDCGADICKIAVMPQSAEDVLTLLSATLKAKQLVAKPIVTMSMGQKGAVSRLAGQT
                     FGSSITFGSGTHNSAPGQIGVSALRAALDCLESGAD"
     gene            2165..4180
                     /gene="rep"
                     /locus_tag="ACFM8S_001783"
     CDS             2165..4180
                     /gene="rep"
                     /locus_tag="ACFM8S_001783"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFC6314817.1"
                     /translation="MMKLNPQQLEAVRYLGGPLLVLAGAGSGKTGVITQKIKHLIVNV
                     GYLPHTVAAITFTNKAAAEMQERVAKMLPKPQTRGLTICTFHSLGMKILREEANHIGY
                     KKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNAWEQ
                     QTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRNKWQRRLRYLLVDECQDTN
                     TCQFTLMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVIKLEQNYR
                     STARILKIANKVIENNPKLFTKKLWSQLGEGDPVKVVACQNEQHEADWVVSQIVKQKL
                     IGGDKTQYADFAVLYRGKHQARIFEEALRGARIPYRLSGGQSFFDKAEIKDVLSYVRL
                     LANPNDDPAFLRAVTTPKRGIGDVTLGKLNTYAHEHECSLYEAAQNEEALATLNNTNR
                     QHLQAFMDMFGSYRAKAETGEAGEFINNLLKEIDYENHLLASEESKAGEIKWRNVGDL
                     VSWFARKGGEDGKNVIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLV
                     GCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGIWQFPEPSRF
                     IDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRLDSMKKGGPED"
BASE COUNT         1006 a         1147 c         1145 g          913 t
ORIGIN      
        1 aaactcaaat caagccgttc ggaggcagct caaaaaacgg tacttcgcag caggagtacc
       61 gtttatcggg atttcaagtt ttactcttcg ggacgctcgc cgtcggtttc gtcctgcgtc
      121 ccttcggtga tgtgcatttc tacgccgttg agggcgcgga tttttgcgtc gatttcgtcg
      181 gcaatttcgg ggttttcttt cagccagacg cggacgttgt ctttgccctg accgattttc
      241 gcgccgttgt agctgtacca cgcgccggat ttgttgataa tgtcgttttt cacgccgatg
      301 tcgatcaatt cgccttccca actgatgcct tctccgtaga ggatgtcaaa ctctgcctga
      361 cggaacgggg gggcgacttt gtttttgatg actttgacgc gggtttcgtt gcccaatacc
      421 tcttcgcctt ttttgatgga tccggtgcgg cggatgtcga ggcggacgga agaatagaat
      481 ttcagcgcgt tgccgccggt ggtggtttcg gggctgccga acattacgcc gatcttcatc
      541 cggatttggt tgatgaacac aaccagcgtg ttggtttttt tgatgtgtcc ggtcagttta
      601 cgcaaagcct gactcatcag gcgcgcctgc agtccgacat ggctgtcccc catatcgcct
      661 tcgatttcgg ctttggggac gagtgcggct acggaatcga cgactaccat atctatgccg
      721 cctgaacgga cgagtgtgtc gcagatttcc aaagcctgtt cgccggtatc gggctgggag
      781 aggtaaagct cttcgacttt tacgccgagt ttgcgggcgt aaacgggatc gaaggcgtgt
      841 tcggcatcga caaaggcgca cacgccgccg tttttctggc attgggcgac ggcttcgagg
      901 cagagggtgg ttttgccgga ggattcgggg ccgaagattt cgacgatgcg cccgcgcggc
      961 agaccgccga ctccgagggc gaggtctaat ccgagcgatc cggtggaaat gacttcgagg
     1021 ttttcttcct gctggctgcc gtccattttc atgatggcac ctttgccgaa acttttttcg
     1081 atttgcgcca gtgcggcggc aagggctttg cttttgtcgt ctgacattgg ggttactccg
     1141 gaacaaatgc ggtatgtggg atgcggcgca acacgggctg cggcgcggga tgtgtatcgt
     1201 tttcccgatg tgcgggctat cggtaatgct gcttcacggg gttgccatta tcgcatattt
     1261 ccttgcttgc cgatatgcgg caggacgcgg cggcttgtgc cggaatggaa tctggatgcc
     1321 gtctaaaagg cggccggctt tgttataatg gcggctgttt tttctgtgtg tgcctgtttt
     1381 atgtgttcct gccttgttgt caaaaatacc gttatcggaa gcggacgcac caaaatcgcc
     1441 gtgccgcttg tcgcccgcga tgccgccgta ctttccgccg tacttgacca aatcaaaaac
     1501 ctgcccttcg atatcgtcga gttccgcgcc gattttttgg aatgcgcggg cagcatcggc
     1561 gaagtattgc gccacacgca gaccgtccgc gacgcgctgc ccgacaaacc gctgctgttt
     1621 acgttcagac ggcatggcga aggcggctca ttcccgtgtt cggacgatta ttattttaaa
     1681 ctgctcgacg cgctgatcga aagtcgcctg cccgacatta tcgatattga gctgttttcc
     1741 ggcgaaaccg ccgtccggtg cgccgtggca aatgctcaaa aaaacggcat cgccgccctg
     1801 ctctgcaatc atgagtttca ccgcacgccg ccgcaagaag aaatcgtatg ccgtctgaaa
     1861 cagatggagg actgcggcgc ggacatctgc aaaattgcgg tgatgccgca aagcgcggaa
     1921 gatgtgctga ctttgctttc tgccacgctc aaagcgaaac agcttgtcgc caaaccgatt
     1981 gttacgatgt cgatggggca aaagggggcg gtcagccgcc ttgccggaca aacgttcggc
     2041 tcaagcatca cattcggttc gggaacgcac aactccgcac cggggcaaat cggcgtatcc
     2101 gccctccgtg cggcactcga ctgtctcgaa agcggcgcag actgatttca gacagcatca
     2161 aaacatgatg aaactcaatc cccaacagct cgaagccgtc cgctacctcg gcggcccact
     2221 gctcgtcctt gccggtgcag gcagcggcaa aaccggcgtg attactcaaa aaattaagca
     2281 tttgattgtc aatgtcggct acctgccgca taccgttgcc gcaattacct ttaccaacaa
     2341 agccgctgcg gaaatgcagg agcgcgttgc caaaatgctg cccaaaccgc aaacgcgcgg
     2401 gctgacgatt tgcacgttcc actctttggg catgaagatt ctgcgcgaag aggcgaacca
     2461 tatcggttac aaaaaaaact tctccattct cgattctacc gacagcgcga aaatcatcgg
     2521 cgaactctta ggcggtacgg gcaaagaagc cgtattcaag gcgcagcacc agatttcctt
     2581 gtggaaaaac gatttaaaaa cgcctgaaga tgtcgttcag acggcctcta atgcgtggga
     2641 acaacaaacc gcacgtgtgt atgcgagcta tcaggaaacc ttacaaagct atcaggcagt
     2701 ggacttcgac gacttaatcc gcctgcctgc cgtgctgttg cagcaaaaca gcgaggtgcg
     2761 caacaaatgg cagcggcggc tgcgttatct gctggttgac gaatgccaag atacgaatac
     2821 ctgccaattt acgttgatga aacttctgac cggcgcggaa ggtatgttta ccgccgtcgg
     2881 cgacgacgac cagtccatct acgcatggcg cggcgcaaac atggaaaatc tgcgcaaaat
     2941 gcaggaaaac tatccacaga tgaaggtcat caaactggag caaaactacc gctccaccgc
     3001 gcggattctc aaaatcgcca acaaagtcat cgaaaacaac cccaagctgt ttaccaaaaa
     3061 actttggtcg caattgggcg aaggcgatcc cgtcaaagtc gttgcctgcc aaaacgagca
     3121 gcacgaagcc gactgggtcg tcagccaaat cgtcaaacaa aaactcatcg gcggcgacaa
     3181 aacccaatac gccgatttcg ccgtgttata ccgcggaaag catcaggcga ggattttcga
     3241 ggaagcattg cgcggcgcgc gcatccccta ccgtctctcc ggcggacaaa gctttttcga
     3301 taaagccgaa atcaaagacg tgttgtctta tgtgcggctg cttgccaacc ccaacgacga
     3361 tcccgccttt ctgcgtgccg ttaccacgcc caaacgcggc atcggcgatg tcacgctggg
     3421 caagctcaac acttacgcgc acgaacacga atgcagcctg tatgaagccg cacaaaacga
     3481 agaagccctt gccacgctga acaataccaa ccgccaacac ctgcaagcct ttatggatat
     3541 gttcggcagc taccgcgcca aagcggaaac cggcgaagca ggcgagttca tcaacaacct
     3601 gcttaaagaa atcgactacg aaaaccatct gcttgccagt gaagaaagca aagcaggcga
     3661 aatcaaatgg cgcaacgtcg gcgatttggt atcatggttt gcgcgaaaag gcggggaaga
     3721 cggcaaaaac gtcatcgaac tcgcccaaac cgtcgccttg atgacgcttt tggaaggaaa
     3781 agacgaagaa gaaaccgatg ctgtctcgct atccacgcta cacgccgcca aaggtttgga
     3841 gtatccgtat gttttccttg tcggttgcga agaaggtgtt ttgccgcaca acgacagcat
     3901 cgaagagggc aacgtcgaag aagaacgccg cctgatgtac gtcggcatca cccgcgccaa
     3961 acgccagctt acgctgaccc actgcgtcaa acgcaaaaaa caaggcatat ggcagttccc
     4021 cgaacccagc cgattcatag acgaaatgcc gcaggaagat ttgaaaatcc tggggcgcaa
     4081 aggcggcgaa ccgattgtca gcaaagaaga aggcagacgc aaccttgccg atataatcgg
     4141 aaggctcgac agcatgaaaa aaggcggtcc ggaggattaa accaagatag ggaaacgaag
     4201 ccggagacaa t
//
LOCUS       DAXHSF010000160         4202 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.160, whole genome shotgun sequence.
ACCESSION   DAXHSF010000160 DAXHSF010000000
VERSION     DAXHSF010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.160"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(308..1204)
                     /gene="argB"
                     /locus_tag="ACFM8S_001784"
     CDS             complement(308..1204)
                     /gene="argB"
                     /locus_tag="ACFM8S_001784"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFC6314818.1"
                     /translation="MESENIISAADKARILAEALPYIRRFSGSVAVIKYGGNAMTEPA
                     LKEGFARDVVLLKLVGIHPVIVHGGGPQINAMLEKVGKKGEFVQGMRVTDKEAMDIVE
                     MVLGGHVNKEIVSMINTYGGHAVGVSGRDDHFIKAKKLLIDTPEQNGVDIGQVGTVES
                     IDTGLVKGLIERGCIPVVAPVGVGEKGEAFNINADLVAGKLAEELNAEKLLMMTNIAG
                     VMDKTGNLLTKLTPKRIDELIADGTLYGGMLPKIASAVEAAVNGVKATHIIDGRVPNA
                     LLLEIFTDAGIGSMILGGGEDA"
     gene            1374..2504
                     /locus_tag="ACFM8S_001785"
     CDS             1374..2504
                     /locus_tag="ACFM8S_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14-type cytosolic carboxypeptidase"
                     /protein_id="HFC6314819.1"
                     /translation="MIKISTRFDAGSVVVKDLTDPSNIRLALRPDNASDFAQWFYFRL
                     QGAAYQNCIMHFENAAEAAYPKGWEGYQACASYDRRNWFRVPTSYENGVLTVNHTPLS
                     NSVYYAYFEPYSEEQHLNLLGDAQGSGLCRIDDLGSTVQGRDINLLTIGNQVESDLKI
                     WITARQHPGETMAEWFVEGLLGRLLDPQDPTARTLLDRATFYIVPNMNPDGSALGNLR
                     TNAAGANLNREWENPTLEKSPEVFFVREKMLETGVDLFLDIHGDEGLPFIFVAGTEGV
                     PNYNPRISALETQFKTALLNASPDFQDEYGYEKDAPGQANMTLATNWVGNRFNCLAYT
                     LEMPFKDNANLPDDDFGWNGQRSLRLGEAALSAILNIIGDLR"
     gene            2575..3426
                     /gene="lgt"
                     /locus_tag="ACFM8S_001786"
     CDS             2575..3426
                     /gene="lgt"
                     /locus_tag="ACFM8S_001786"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFC6314820.1"
                     /translation="MITHPQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQG
                     LSVFTKESLDDFLTWGILGVILGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGF
                     LGVVIAIWLFGRKHGIGFLKLMDTVAPLVPLGLASGRIGNFINGELWGRVTDINAFWA
                     MGFPQARYEDLEAAAHNPLWAEWLQQYGMLPRHPSQLYQFALEGICLFAVVWLFSKKQ
                     RPTGQVASLFLGGYGIFRFIAEFARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVR
                     FGMKKQH"
     gene            complement(3509..4171)
                     /locus_tag="ACFM8S_001787"
     CDS             complement(3509..4171)
                     /locus_tag="ACFM8S_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HFC6314821.1"
                     /translation="MDSRKFTEASKRRLSELLDAKSEQGNTMRCDEVQGFMTALLSGP
                     DKLTPLDWLPEVLGDESQFTAAERSEIERLVLAMAMETTAAMSDKKLPDLWLYENEDG
                     GSDFYTWCNAYLYGLDIVPTDWFEAVDDEAFEELFYPIMALGGIYDEEDNGTIRLQFT
                     EGELAELESELPYALADIYRYWQAVINKPQTVRREGEKTGRNDPCPCGSGRKYKACCG
                     KN"
BASE COUNT          941 a         1223 c         1018 g         1020 t
ORIGIN      
        1 tctaaagaaa ccttttttcc cgataagttt ccgtgccgac aggtctagat tcccgcctgc
       61 gcgggaatga cgaaatttca aagttatggc gttatcggaa aaacaaaaaa tcaaagccgg
      121 agaatttatc ccaaacaacc ggatttcaaa aaaaccagat tcccgccttc gcgggaatga
      181 cggattttag gtttctgttt tggtttctgt tttcgcggga ataacaactt agaaattgcc
      241 cgaaacccca aaaacagaaa ccggacaaac gggttttccg ccaaagccgg cattttccga
      301 ctttgcttca ggcatcttcc ccaccgccca aaatcatcga accgataccg gcatcggtaa
      361 agatttccag caaaagcgcg ttgggcaccc tgccgtcgat gatatgcgtg gctttcacgc
      421 cgttgacggc ggcttcgacc gcagaagcga ttttcggcag cataccgcca tacagcgtgc
      481 cgtcggcaat cagttcatca atccgtttcg gcgtgagttt ggtcagcaga ttgcccgttt
      541 tgtccatcac accggcgata ttcgtcatca tcaagagttt ttcggcgttc aattcttccg
      601 ccaatttgcc tgctaccaaa tcggcgttga tgttgaacgc ttcgcctttt tcacctacgc
      661 cgacgggggc gacgacggga atgcagccac gttctatcag ccctttaacc aaaccggtat
      721 cgatgctttc caccgtaccg acctgtccga tgtccacgcc attctgttcg ggcgtatcga
      781 tcaaaagttt cttcgcctta atgaaatggt cgtcgcgtcc gcttacgccg accgcgtgtc
      841 cgccatatgt gttaatcatc gacacgattt ctttattgac atgcccgccc aacaccattt
      901 cgacaatatc catcgcctct ttgtcggtaa cgcgcattcc ttggacaaac tcaccctttt
      961 tgccgacttt ttcaagcatc gcattgatct gcggcccgcc gccgtgaacg atgacgggat
     1021 gaatgccgac cagcttcagc agcacgacat cgcgggcaaa cccttctttc aaggcaggtt
     1081 cggtcatcgc gttgccgccg tatttgatga cggcgaccga accggaaaac cggcggatgt
     1141 aaggcagcgc ttcggcaagg atacgcgcct tgtcggcggc ggaaataatg ttttcagact
     1201 ccataacggc tccctattgt tgacaatttc ggcttatgtt aacgccaaac gccgtctgaa
     1261 acaatcttca gacggcatcc ggcagacttt cgagcaggtt gaagccttgc gatacgggcg
     1321 gtaaaatcgc ggtttatccg gttcgagaaa ccggcgacag caaggacttt cccatgatta
     1381 aaatcagcac ccgcttcgat gccggctcgg tcgtcgtcaa agacctgacc gatccttcca
     1441 acatccgcct cgccctgcgt cccgacaacg cctccgattt tgcacaatgg ttctacttcc
     1501 ggctgcaagg cgcggcatat caaaactgca tcatgcattt tgaaaacgcg gcagaagccg
     1561 cctacccgaa aggctgggaa ggctatcagg cgtgtgcctc atacgaccgc cgcaactggt
     1621 tccgcgtacc gacttcctac gaaaacggcg tactgaccgt caatcatacg ccgctgtcca
     1681 acagcgtgta ttacgcctat ttcgaacctt actccgaaga acagcattta aacctcctcg
     1741 gcgacgcgca aggcagcggc ctgtgccgca tcgacgactt gggcagcacc gtgcaagggc
     1801 gcgacatcaa tctgctgacc atcggcaatc aggtcgaaag cgatctgaaa atctggatta
     1861 ccgcccgcca gcatccgggc gaaaccatgg cggaatggtt tgtcgagggg ctgctcggca
     1921 ggctgctcga tccgcaagac ccgacagcgc gcaccctgct cgaccgcgcc acgttctata
     1981 tcgtccccaa tatgaacccc gacggttcgg cactgggcaa ccttcgtacc aacgccgccg
     2041 gtgcgaacct caaccgcgaa tgggaaaatc cgactttgga aaaaagcccc gaagtgttct
     2101 tcgtgcgcga aaaaatgctg gaaaccggcg tggatttgtt cttggacatc cacggcgacg
     2161 aagggctgcc ctttatcttt gttgcaggaa ctgaaggcgt gccaaactac aatccgcgca
     2221 tttccgcgtt ggaaacacag ttcaaaaccg cccttttgaa cgccagcccc gatttccaag
     2281 acgaatacgg ttacgaaaaa gatgcgcccg gtcaggcaaa catgaccttg gcaacaaact
     2341 gggtcggcaa ccgtttcaac tgccttgcct atacgttgga aatgccgttc aaagacaatg
     2401 ccaacctgcc cgacgacgat ttcggatgga acggtcaacg gtcgttgcgc ttgggcgaag
     2461 ccgcgttgtc cgccatcctc aacatcatcg gcgatttgcg ctgaaaccct tattgcagaa
     2521 atgccgtctg aagcatcatg cctttcagac ggcatttgaa aacgaaagcc cgttatgatt
     2581 acccatcccc aattcgaccc cgtccttatc agtatcggcc cgcttgccgt ccgctggtat
     2641 gccctaagct acatcctcgg atttattctt tttacctttc tcggcagaag gcgcatcgcg
     2701 caaggcttgt ccgtttttac caaagaatcg ctcgacgact tcctgacatg gggcattttg
     2761 ggcgtaattt tgggcgggcg tttgggttac gtcctgtttt acaagttttc cgactacctc
     2821 gcccatccgc ttgatatttt caaggtatgg gaaggcggaa tgtcgttcca cggcggcttt
     2881 ttgggtgtag ttattgccat atggttgttc ggtcgcaaac acggcatcgg cttcctcaaa
     2941 ctgatggaca cggtcgcacc gctcgttcca ctgggtctcg cttcgggacg tatcggcaac
     3001 ttcatcaacg gcgaactttg gggacgcgtt accgacatca acgcattttg ggcaatgggc
     3061 ttcccgcagg cgcgttacga agacctcgaa gccgccgcgc acaatccgct ttgggcagaa
     3121 tggctgcaac aatacggtat gctgccgcgt catccctcgc agctttatca gtttgcactt
     3181 gaaggcatct gcctgttcgc cgtcgtttgg ctgttctcta aaaaacagcg gccgaccgga
     3241 caagtcgcct cactcttcct cggcggctac ggcatattcc gcttcattgc cgaatttgca
     3301 cgccaacccg acgactatct cgggctgctg accttggggc tgtcgatggg gcaatggttg
     3361 agcgtcccga tgattgtttt gggtatcgtc ggctttgtcc ggttcggcat gaaaaaacag
     3421 cactgaccgc ctgtgaaaaa taccgtctga aacatggaaa tccggtttca gacggcattt
     3481 ttacgtttgg ttcatggaaa caaacgcttc aattcttacc gcaacacgcc ttgtattttc
     3541 tgccgctgcc gcacggacag ggatcgttcc tgcctgtttt ttcgccttcc ctgcggacgg
     3601 tttgcggctt gttgatgacc gcctgccagt agcggtagat atccgccaat gcataaggca
     3661 actcggattc cagttccgcc agctcgcctt ctgtgaattg cagacggata gtgccgttgt
     3721 cctcttcgtc gtaaataccg cccaatgcca tgatgggata aaacaactct tcaaacgctt
     3781 catcatcgac ggcttcaaac caatcggtcg gcacaatatc caaaccgtaa agataagcat
     3841 tgcaccatgt gtaaaaatcg ctgccgccgt cttcgttttc atacagccac aaatcgggca
     3901 gttttttatc cgacatcgcg gcggttgttt ccatcgccat tgccaaaacc agccgttcga
     3961 tttcggaacg ttcggcggcg gtaaattgcg attcgtcgcc caacacttcg ggcagccagt
     4021 cgagcggtgt caatttgtcc ggcccgctca acagcgccgt cataaaacct tgaacctcgt
     4081 cgcaacgcat cgtgttgcct tgttcgcttt tggcatccaa caattcgctc aaccgccgtt
     4141 tggatgcttc ggtaaatttt cgggaatcca tcattttcct tttccaatgg gttttgcgcc
     4201 ct
//
LOCUS       DAXHSF010000161         4154 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.161, whole genome shotgun sequence.
ACCESSION   DAXHSF010000161 DAXHSF010000000
VERSION     DAXHSF010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.161"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..220
                     /locus_tag="ACFM8S_001788"
     CDS             <1..220
                     /locus_tag="ACFM8S_001788"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314822.1"
                     /translation="ERYWKMKEMKPVRYHIGDMPETSKQTASRHDDRAVGVDDDCFHD
                     SFGQFLFGCSAEATRTAPISVKRSCLFT"
     gene            328..1377
                     /gene="lpxK"
                     /locus_tag="ACFM8S_001789"
     CDS             328..1377
                     /gene="lpxK"
                     /locus_tag="ACFM8S_001789"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFC6314823.1"
                     /translation="MPNCFKFHTLIERHWQKPYPVLSFLLKPLSGLFAKIAVKRRVDF
                     LSGKRQSEKLSVPVVVVGNIHAGGTGKTPIVAALVSGLQEKGVKVGIISRGYGRKSKA
                     VHVLNAESRAEDAGDEPLLLFRKTGAPTAVGSSRAEAGRALLAAHPDIGLIVADDGLQ
                     HYALRRDVEIAVFPAADTGRTDLDLLPNGSLREPLLRLDSVDAVVVSGGKADALFRPS
                     ENMFHSRIEAGRIYRLNNPSEILDTGRLKNQTVVAVAGIAKPARFFDSLRNMGITVKR
                     TVALPDHADISAADLPDADAVIITEKDAVKFSDGICTDNVWVLPVCAIIEPDLAAFVL
                     ERLEGAEGRLKARFG"
     gene            1567..2148
                     /locus_tag="ACFM8S_001790"
     CDS             1567..2148
                     /locus_tag="ACFM8S_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HFC6314824.1"
                     /translation="MKLKTLLLPFATLALCTNAFAAPPSDASLARWLDTQNFDRDIEK
                     NMIEGFNAGFKPYADKALAEMPEAKKDQAAEAFNRYRENVLKDLITPEVKQAVRNTLL
                     KNAREIYTQEEIDGMIAFYGSPVGQSVVAKNPRLIKKSMSEIAVSWTALSGKIARHHL
                     PAFTEELRRIICGGKNPDAGCKQAGQVGKRHQK"
     gene            2217..2399
                     /locus_tag="ACFM8S_001791"
     CDS             2217..2399
                     /locus_tag="ACFM8S_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HFC6314825.1"
                     /translation="MEKKFLDILVCPVTKGRLEYHQDKQELWSRQAKLAYPIKDGIPY
                     MLENEARTLSEEELKA"
     gene            2396..3157
                     /gene="kdsB"
                     /locus_tag="ACFM8S_001792"
     CDS             2396..3157
                     /gene="kdsB"
                     /locus_tag="ACFM8S_001792"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFC6314826.1"
                     /translation="MTEFVVLIPARLDSSRLPRKALADIHGKPMVVRVAEQAAKSKAA
                     RVVVATDHPDIQTVCQAHGVEVVMTSNRHESGTTRLAEAAAALKLPQHLVVVNVQGDE
                     PLIAPELIDRTAEVLVENNVQMATAAHELHDFDELMNPNAVKVVLDKNRNAIYFSRAP
                     IPYLRDAMRAGKREMPSETAVLRHIGIYAYRAGFLQRYAEMSVSPLETIESLEQLRVL
                     WHGYPIAVETAKEAPAAGVDTQEDLDRVRAVFQTV"
     gene            3180..3497
                     /locus_tag="ACFM8S_001793"
                     /pseudo
     CDS             3180..3497
                     /locus_tag="ACFM8S_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            3599..3799
                     /locus_tag="ACFM8S_001794"
                     /pseudo
     CDS             3599..3799
                     /locus_tag="ACFM8S_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            3956..4048
                     /locus_tag="ACFM8S_001795"
     tRNA            3956..4048
                     /locus_tag="ACFM8S_001795"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3990..3992,aa:Ser,seq:gct)
BASE COUNT         1034 a         1003 c         1129 g          988 t
ORIGIN      
        1 tgaaagatac tggaaaatga aagagatgaa acctgtccgt tatcatattg gcgatatgcc
       61 cgagacttca aaacaaaccg cctcccgtca tgacgacagg gcagtgggtg ttgacgatga
      121 ttgttttcat gattcctttg gtcaattttt gtttgggtgt tcggcagagg caacccgaac
      181 cgcgccaatt tctgtaaagc gcagttgctt atttacctga ttggttcgct tatcggtttg
      241 gtcttcgcgt tgtttatagg tgggtctgta tcgggtacgc atgattaatg ccccgggctg
      301 attttgcttc gaggatttgt atcgaatatg ccgaattgtt tcaaatttca tacccttatc
      361 gaacggcatt ggcaaaaacc ttatccggtt ttgtcttttc tgcttaagcc gctctccggg
      421 ctgtttgcca aaattgcggt aaaacggcgg gtggattttt tatcgggaaa acggcaaagc
      481 gaaaagctgt ccgtgcctgt ggtcgtggtc ggcaatattc acgcgggtgg gacggggaaa
      541 acgccgattg ttgccgcgct ggtgtcgggt ttgcaggaaa agggcgtcaa ggtcggcatc
      601 atcagccgag gctacgggcg caagagcaag gcggttcatg tattgaatgc tgagagccga
      661 gcggaagatg cgggcgatga gcctttgctg ctgttccgca aaaccggtgc gccgacggcg
      721 gtgggcagca gccgtgcaga ggcaggcagg gcgttgctgg cggcgcatcc cgacatcgga
      781 ctgattgtgg cggacgacgg tttgcagcat tacgccctgc ggcgagatgt ggaaatcgcg
      841 gtgtttccgg cggcggatac ggggcgcacg gatttggatt tactgcccaa cggcagtttg
      901 cgcgaacctt tgttgcggct ggattcggtg gatgcggtcg tcgtcagcgg cggcaaggcg
      961 gatgcgctgt ttaggccgtc tgaaaatatg tttcacagcc gtatcgaagc gggacggatt
     1021 taccgtttga acaatccgtc cgaaatactg gacacaggcc gtctgaaaaa tcaaaccgtc
     1081 gtcgccgtgg caggtattgc caagccggcg cggttttttg attcgttgcg gaatatgggt
     1141 attaccgtga agcgaaccgt cgcgctgccc gaccacgccg acatttcggc ggcagatttg
     1201 cccgatgcgg acgcggtcat tattacggag aaagatgcgg tcaaattttc agacggcatt
     1261 tgcaccgata atgtttgggt gttgcccgtt tgtgcgataa tcgaacctga tttggcggcg
     1321 tttgtgttgg agcggttgga aggtgccgaa ggccgtctga aagcgcggtt tggatgaagt
     1381 gataacggac tggaataaga acgccctacg ccgtcattcc cgcgcaggtg ggaatctgga
     1441 tcttgatttt tcagaaatga ccaagaggtt tccgaagtcc taaatctccg gattcccgcc
     1501 tgcgcaggaa taatggaagc cggttgtatt ttttatctgc attaatcatt cattaaagga
     1561 ttgaatatta aactgaaaac cttgttattg cccttcgcca cgctggcatt gtgcaccaac
     1621 gcttttgccg ccccgcccag cgacgcgtcg ttggcgcgtt ggctggatac gcagaatttt
     1681 gaccgggata tagaaaaaaa tatgattgag ggctttaatg ccggatttaa accgtatgcg
     1741 gacaaagccc ttgccgaaat gccggaagcg aaaaaagatc aggcggcaga agcctttaac
     1801 cgttatcgtg agaatgtttt gaaagatttg attacgcccg aagtgaaaca ggctgtccgt
     1861 aatactttat tgaagaatgc ccgtgagata tacacgcaag aagaaattga cggcatgatt
     1921 gccttttacg gttcgcctgt cggtcagtcc gtcgttgcca aaaatccgcg cttaatcaag
     1981 aaatcgatga gtgaaatagc agtatcttgg actgcattgt cagggaaaat cgcgcgacat
     2041 catctgcccg cgtttacgga agagttgcgg cgcatcatct gcggcggtaa aaatcccgat
     2101 gcgggctgta aacaagccgg acaggttggg aaaaggcatc agaaataaat gatagccgtc
     2161 tgaaatattg aagagggcat ccgattgatt gaaccatcaa acccgaaagc aaccctatgg
     2221 aaaaaaaatt cttagacatc ctcgtctgcc ccgttaccaa aggcaggctg gaatatcatc
     2281 aggacaaaca ggaattgtgg agccgtcagg cgaagcttgc ctatccgatt aaagacggca
     2341 ttccctatat gctggaaaac gaagcgcgaa cgttgagcga agaggaactc aaagcatgac
     2401 cgaattcgtc gtattgattc cggcgcggct ggattcgtcg cgcctgccca gaaaagcctt
     2461 ggcggacatc cacggcaaac cgatggtcgt gcgcgttgcc gaacaggcgg caaaaagtaa
     2521 agctgcgcgc gtcgttgttg ccaccgacca tcccgatatt cagacggtct gtcaggcgca
     2581 cggcgtcgaa gtcgtcatga cttcaaaccg gcacgaaagc ggcacgacgc gccttgctga
     2641 agctgccgcc gcgctgaagc tgccgcaaca tttggtcgtt gtcaacgtac agggcgacga
     2701 gccgctgatt gcccccgaac tcatcgaccg caccgccgaa gtactcgtcg aaaacaacgt
     2761 ccaaatggcg accgccgccc acgaattgca cgatttcgac gaattgatga atcccaatgc
     2821 cgtcaaagtc gtcctcgaca aaaaccgcaa cgccatctac ttcagccgcg ccccgattcc
     2881 ctatctgcgc gatgcaatgc gtgccggaaa acgcgaaatg ccgtctgaaa ccgccgtcct
     2941 gcgccatatc ggcatctacg cttaccgcgc cggcttcctg caacgctatg ccgaaatgag
     3001 cgtttcgccg ctggaaacca tcgaatcgct ggaacagctg cgcgtcctgt ggcacggtta
     3061 tcccattgcc gtcgaaaccg ccaaagaagc ccccgccgcc ggtgtggata cgcaggaaga
     3121 tttggatagg gttcgcgctg tatttcagac cgtataaaac aggttcaaag ggaaaagata
     3181 tgcagcaaca tattgaaaag tggcaacact tgagccggga agaacagaaa atccttgccg
     3241 aagtatgggg tctcgtgcaa aacgatgatc aggaggttca ctatgaaatg cttaaattga
     3301 acgcacccga cgaagccagt ggcgaatttt ggttcagaat ggcagaaaca ctcagcaccc
     3361 tgccgcccaa ccgttccctc gaccttagaa tgaacggcgg caggctggcg accgccgtat
     3421 ccatcctttc cgtcatgatt gaagacaatc ccgacatacc gcagctttgg gcgcaaaaaa
     3481 ttaccgccct caattatagt ggattaaatt taaaccagta cggcgttgcc tcgccttgtc
     3541 gtactatctg tactgtctgc ggcttcgtcg ccttgtcctg atttttgtta atccactata
     3601 tttggcacac ggacacaaag cccgtgccga cggtttggca caacagcccg acaaagcggc
     3661 agaagccaac gaggaggaat acctgaccaa agccctgtcg caaaacctgc tgtcaacatt
     3721 ggatgtcgcg cttgcacgtt ttcctgaaga cgcgtggttt caggaaatca aacaggatgc
     3781 acaaaagcat tttgcttgag gatgtggcag tcaggaatat ttccattcag gaagaaaaga
     3841 agtgcctgat tgggtataat cagggtaaat cttattttat ttcaaaagat taatatttgc
     3901 tttccatttt tccttgacgg tatcggaaaa gttgattata gttacagctt ccttaggagt
     3961 aatggctgag aggctgaagg cacttccctg ctaaggaagc atgtggggtc aacctgcatc
     4021 gagggttcga atccctctta ctccgccaga taaaaaatag acgctgtgtt ttacagtgtc
     4081 tattttttta tgcaatttta tagcgggttg gtgcaaaacc agtatggtat tgccctgtct
     4141 tgattctgaa tttt
//
LOCUS       DAXHSF010000162         4128 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.162, whole genome shotgun sequence.
ACCESSION   DAXHSF010000162 DAXHSF010000000
VERSION     DAXHSF010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.162"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            43..675
                     /locus_tag="ACFM8S_001796"
     CDS             43..675
                     /locus_tag="ACFM8S_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFC6314827.1"
                     /translation="MENTNRVPEQARYDAERRQADEALAGVFPAVSIFGSARTPQNHA
                     DYAFACRLARRLSDSGIAVISGGGPGIMEAANKGAFAGKSVSVGLNIVLPHEQKPNPY
                     QDIALRFSRFAERKAVFFRYSQAYVVMPGGFGTLDELFEILTLVQTGKVPPRPIVLVG
                     KAFWSGLAEWINAQLLARGLISEGAVSLFAISDDEDEIVAYLSEHGLQTA"
     gene            747..2117
                     /gene="purB"
                     /locus_tag="ACFM8S_001797"
     CDS             747..2117
                     /gene="purB"
                     /locus_tag="ACFM8S_001797"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFC6314828.1"
                     /translation="MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLK
                     ALAAEPKIVEVPPFSAETLAEIDTVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKER
                     FSGVPEVAAASEFIHFACTSEDINNLSHALMLQEAREAVLLPKLAEIIEKLTAMAHDL
                     AAVPMMSRTHGQPATPTTLGKETANVVYRLQRQFKNLQAQEFLGKINGAVGNYNAHMV
                     AYPDVDWETHCRNFVEISLGLTFNPYTIQIEPHDYMAEFFQTLSRINTILIDFNRDVW
                     GYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMSNAVLGFLSEKLPISRWQ
                     RDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDATWELLAEPIQTV
                     MRRYGVADPYEKLKDLTRGKGGITPEVLKVFIGSLEIPTEAKAKLLELTPALYVGKAE
                     ALAKRI"
     gene            2198..4066
                     /locus_tag="ACFM8S_001798"
     CDS             2198..4066
                     /locus_tag="ACFM8S_001798"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFC6314829.1"
                     /translation="MQAVRYRPEIDGLRAVAVLSVIIFHLNNRWLPGGFLGVDIFFVI
                     SGFLITGIILSEIQNGSFSFRGFYTRRIKRIYPAFIAAVSLASVIASQIFLYEDFNQM
                     RKTVELSAVFLSNIYLGFQQGYFDLSADENPVLHIWSLAVEEQYYLLYPLLLIFCCKK
                     TKSLRVLCHISIILFLILTASSFLPAAFYTDILNQPNTYYLSTLRFPELLAGSLLAVY
                     GQTQNGRRQTANGKRQLLSSLCFGALLACLFVIDKHNPFIPVMTLLLPCLLTALLIRS
                     MQYGTLPTRILSASPIVFVGKISYSLYLYHWIFIAFAHYITGDKQLGLPAVSAVAALT
                     AGFSLLSYYLIEQPLRKRKMTFKKAFFCLYLAPSLILVGYNLYARGILKQEHLRPLPG
                     APLAAENHFPETVLTLGDSHAGHLRGFLDYVGSREGWKAKILSLDSECLVWVDEKLAD
                     NPLCRKYRDEVEKAEAVFIAQFYDLRMGGQPVPRFEAQSFLIPGFQARFRETVKRIAA
                     VKPVYVFANNTSISRSPLREEKLKRFAANQYLRPIQAMGDIGKSNKAVFDLVKDIPNV
                     HWVDAQKYLPENTVEIHGRYLYGDQDHLTYFGSYYMGREFHKHERLLKSSRGGALQ"
BASE COUNT          925 a         1114 c         1074 g         1015 t
ORIGIN      
        1 tttttgacgg tgtacaatcg ctgtttttga acggaggatg gaatggagaa tacaaaccgt
       61 gtgccggagc aggcacgtta tgatgccgaa cgcaggcagg cagacgaagc attggcgggc
      121 gtgtttccgg cagtcagtat cttcggcagc gcgcgcacgc cgcagaatca tgcggattat
      181 gcgttcgcct gccgtctggc gcggcggctg tcggattcgg gcattgccgt catttcgggc
      241 ggcgggccgg ggattatgga ggcggcaaac aagggcgcgt ttgcagggaa gtcggtttcg
      301 gtggggctga acatcgtttt gccgcacgag cagaaaccga atccgtatca ggacatcgcc
      361 ttgcggtttt cccgttttgc cgaacgcaag gcggtgtttt tccgctattc ccaagcatat
      421 gtcgtgatgc cgggcggctt cgggacgctg gacgaattgt ttgaaatcct gaccttggtg
      481 cagacgggca aagtgccgcc gcgtccgatt gttttggtcg gaaaggcgtt ttggtcgggc
      541 ttggcggagt ggataaacgc gcagcttttg gcgcgcggtc tgatttccga aggggcggtc
      601 tctttgtttg ccatatcgga cgatgaagac gaaatcgttg cgtatctgtc ggaacacggg
      661 cttcagacgg catagcgtcc tgagagtgat gtataattgc aaacaattta acaatttttg
      721 atgtctttcc cgaacaggat gccgaaatga tcaaccccat cgcctcgctt tcccctttag
      781 atggccgtta tgcccaatcc gttgaagcat tgcgcccgat tttttcagag tacggcctga
      841 tgaaggcgcg cgtcaaagtc gaattaaact ggctcaaagc cctggccgcc gagccgaaga
      901 ttgtcgaagt gccgcccttc agtgccgaaa cgcttgccga aatcgacacg gtgattgaaa
      961 acttttcatt ggaagacgca gccgctgtca aagccatcga agccacgacc aatcatgatg
     1021 tcaaagccat tgaatattgg cttaaagaac gcttctccgg cgtgcctgaa gtggcagctg
     1081 ccagcgagtt catccacttt gcctgcacca gcgaagacat caacaacctg tcccacgctt
     1141 taatgctgca agaagcgcgt gaggctgttt tgctgccgaa gctggccgaa atcatcgaaa
     1201 aactgaccgc tatggcgcac gaccttgccg ccgtcccgat gatgagccgc acccacggcc
     1261 agcccgccac gccgaccact ttgggcaaag aaaccgccaa tgtcgtgtac cgcctgcaac
     1321 gccagtttaa aaacctgcaa gcgcaagagt tcctcggcaa aatcaacggc gcggtcggca
     1381 actacaacgc ccatatggtc gcctatcctg atgtagattg ggaaacccac tgccgcaact
     1441 tcgttgaaat cagcctcggc ctgaccttca acccctacac cattcaaatc gagccgcacg
     1501 actatatggc ggagtttttc caaaccctca gccgcatcaa cacgattctc atcgacttta
     1561 accgcgacgt ttggggttat atttcattgg gttacttcaa acaaaaagtc aaagcgggcg
     1621 aagtcggttc ttccaccatg ccgcacaaag tcaaccccat cgactttgaa aactccgaag
     1681 gcaacctcgg catgtctaac gccgtattgg gctttttgtc cgaaaaactg ccgatttccc
     1741 gctggcagcg cgacctgacc gacagcaccg tcttacgcaa tatgggcgta ggcgtgggct
     1801 atgccgtgtt gggtttcgcc gcccacctgc gcggtctgaa caagctggaa cccaaccctg
     1861 ccgcgcttgc cgccgatttg gatgccactt gggaactgct cgccgagccg attcaaaccg
     1921 tgatgcgccg ttacggtgtc gccgatcctt acgaaaaact gaaagacctg acgcgcggca
     1981 aaggcggcat tacgcccgaa gtgctgaaag tctttatcgg atcgctggaa atccctaccg
     2041 aagccaaagc aaaattgctt gagctgaccc ccgcactgta tgtgggcaag gctgaagcgt
     2101 tggcgaaacg gatttgagcg tttattgaaa ccgatgccgt ctgaacgcgc gttcagacgg
     2161 catttttaag ataacgggac atacgggggc gatattgatg caagctgtcc gatacaggcc
     2221 tgaaattgac ggattgcggg ccgttgccgt gctatccgtc attattttcc acctgaataa
     2281 ccgctggctg cccggagggt ttttgggggt ggacattttc tttgtcatct cgggattcct
     2341 cattaccggc atcattcttt ctgaaataca gaacggttct ttttctttcc ggggttttta
     2401 tacccgcagg attaagcgga tttaccctgc ctttattgcg gccgtgtcgc tggcttcggt
     2461 gattgcctct caaatcttcc tttatgaaga tttcaaccaa atgcggaaaa ccgtggagct
     2521 ttctgcggtt ttcttgtcca atatttattt ggggtttcag caggggtatt tcgatttgag
     2581 tgccgacgag aaccccgtac tgcatatctg gtctttggcg gtagaggaac agtattacct
     2641 cctgtatcct cttttgctga tattttgctg caaaaaaacc aaatcgctac gggtgttgtg
     2701 ccacatcagt atcatcctgt tcctgattct gactgcctca tcgtttttgc cggccgcgtt
     2761 ttataccgac atcctcaacc aacccaatac ttattacctt tcgacactga ggtttcccga
     2821 gctgttggca ggttcgctgc tggcggttta cgggcaaacg caaaacggca gacggcaaac
     2881 agcaaatgga aaacggcagt tgctttcatc actctgcttc ggcgcattgc ttgcctgcct
     2941 gttcgtgatt gacaaacaca atccgtttat cccggtaatg accctgctcc ttccctgcct
     3001 gctgacggca ctgcttatcc ggagtatgca gtacgggacg cttccgaccc gcatcctgtc
     3061 ggcaagcccc atcgtatttg tcggcaaaat ctcttattcc ctatacctgt accattggat
     3121 ttttattgct ttcgcccatt acattacagg cgacaaacag ctcggactgc ctgccgtatc
     3181 ggcggttgcc gcgttgacgg ccggattttc cctgttgagt tattatttga ttgaacagcc
     3241 gcttagaaaa cggaagatga ccttcaaaaa ggcatttttc tgcctctatc tcgccccgtc
     3301 cttgatactt gtcggttaca acctgtacgc aagggggata ttgaaacagg aacacctccg
     3361 cccgttgccc ggcgcgcccc ttgctgcgga aaatcatttt ccggaaaccg tcctgaccct
     3421 cggcgactcg cacgccggac acctgcgtgg gtttctggat tatgtcggca gccgggaagg
     3481 gtggaaagcc aaaatcctgt ccctcgattc ggagtgtttg gtttgggtag atgagaagct
     3541 ggcagacaac ccgttgtgcc gaaaataccg ggatgaagtt gaaaaggctg aagccgtttt
     3601 cattgcccaa ttctatgatt tgaggatggg cggccagccc gtgccgagat ttgaagcgca
     3661 atccttccta atacccgggt tccaagcccg attcagggaa accgtcaaaa ggatagccgc
     3721 cgtcaaaccc gtctatgttt ttgcaaacaa cacatcaatc agccgttcgc ccctgaggga
     3781 ggaaaaattg aaaaggtttg ccgcaaacca atatctccgc cccattcagg ctatgggcga
     3841 catcggcaag agcaataaag cggtttttga tttggttaaa gatatcccca atgtgcattg
     3901 ggtggacgca caaaaatacc tgcctgaaaa cacggtcgaa atacacggcc gctatcttta
     3961 cggcgaccaa gaccacctga cctatttcgg ttcttattat atggggcggg aatttcacaa
     4021 acacgaacgc ctgcttaaat cttcccgcgg cggcgcattg cagtagcctg ccttcttgtc
     4081 ggatattgcc tttggcagcc tatgccgctg tttgccgttc ggggcggc
//
LOCUS       DAXHSF010000163         4000 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.163, whole genome shotgun sequence.
ACCESSION   DAXHSF010000163 DAXHSF010000000
VERSION     DAXHSF010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.163"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(67..1317)
                     /gene="glyA"
                     /locus_tag="ACFM8S_001799"
     CDS             complement(67..1317)
                     /gene="glyA"
                     /locus_tag="ACFM8S_001799"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFC6314830.1"
                     /translation="MFSKSVTLAQYDPDLAAAIAQEDQRQQDHVELIASENYVSCAVM
                     EAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRVKKLFGAQYANVQPHSGSQAN
                     QAVYASVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
                     LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLVAGGEYPNPVP
                     FCDFVTTTTHKTLRGPRGGVILCRDNTHEKALNSSIFPSLQGGPLMHVIAAKAVAFKE
                     ALQPEFKQYAKQVKINAAAMAEELVKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAA
                     LGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNEADARVLANLVADVLSNPE
                     DEANLAKVREQVTALCNKYPVYGA"
     gene            complement(1472..1735)
                     /locus_tag="ACFM8S_001800"
     CDS             complement(1472..1735)
                     /locus_tag="ACFM8S_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314831.1"
                     /translation="MSNQTKIQAIKQASEQIFAICETPNTALQAIHLILRHGGAGELS
                     WQVVYQRVMADKDVVGAGYLIDFAQTAENLPFDALPLGSLVLT"
     gene            complement(1798..3573)
                     /gene="ggt"
                     /locus_tag="ACFM8S_001801"
     CDS             complement(1798..3573)
                     /gene="ggt"
                     /locus_tag="ACFM8S_001801"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFC6314832.1"
                     /translation="MAKTYLLTALIMSMTISGCQVIHANQGKVNTHSAVITGADAHTP
                     EHATGLIEQKQVIASDFMVASANPLATQAGYDILKQGGSAADAMVAVQTTLSLVEPQS
                     SGLGGGAFVLYWDNTAKTLTTFDGRETAPMRATPELFLDKDGQPLKFMEAVVGGRSVG
                     TPAIPKLMETIHQRYGVLPWGKLFDTPIRLAKQGFEVSPRLAISVEQNQQHLARYPKT
                     AAYFLPNGVPLQAGSLLKNLEFADSVQALAAQGAKALHTGKYAQNIVSVVQNAKDNPG
                     QLSLQDLSDYQVVERPPVCVTYRIYEVCGMGAPSSGGIAVGQILGILNEFSPNQVGYD
                     AEGLRLLGDASRLAFADRDVYLGDPDFVPVPIRQLISKDYLKHRSQLLEQSDKALPSV
                     SAGDFIHEWVSSQAIELPSTSHISIVDKAGNVLSMTTSIENAFGSTLMANGYLLNNEL
                     TDFSFEPIKQGKQVANRVEPGKRPRSSMAPTIVFKAGKPYMAIGSPGGSRIIGYVAKT
                     IVAHSDWNMDIQDAISAPNLLNRFGNYELETGTTALQWQQALNDLGYKTDVRELNSGV
                     QAIIIEPSRLVGGADPRREGRVMGD"
     gene            complement(3741..4000)
                     /locus_tag="ACFM8S_001802"
                     /pseudo
     CDS             complement(3741..4000)
                     /locus_tag="ACFM8S_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116440.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
BASE COUNT         1005 a          954 c          937 g         1104 t
ORIGIN      
        1 tccgcctgaa acattatgag atttcaggcg gcattggatt gcttagcgga atatttttaa
       61 aaaggcttac gcgccgtaaa cggggtattt attgcacaaa gcagttactt gttcgcggac
      121 tttggcgagg ttggcttcgt cttcggggtt agacaatacg tcggcaacca agttcgccaa
      181 tacgcgcgcg tcggcttcgt taaaaccgcg tgtggtcatg gcggcggaac cgatgcggat
      241 gccggaggta acgaagggtt tttccggatc attcggaatg gcgtttttgt tgacagtgat
      301 gtgcgctttg cccaaagcag cttcggcggc tttgccggta attttcatcg gttgcaggtc
      361 aacgaggaaa acgtggcttt cggtgcggcc ggaaacgatg cgcaaaccgc gtttaaccaa
      421 ctcttccgcc atggcggctg cattgatttt cacttgtttt gcgtattgtt tgaactcggg
      481 ttgcaatgct tctttaaacg ccacggcttt ggcggcgata acgtgcatca gcgggccacc
      541 ttgcaggctt gggaagatgg aagagttcaa cgctttttcg tgggtattgt cgcggcacaa
      601 aattacgccg ccgcgaggac cgcgcagggt tttgtgggtg gtggtggtaa cgaagtcgca
      661 gaacggcacg ggattgggat attcgccgcc ggcaaccaga ccggcatagt gcgccatatc
      721 gacaaagagg tatgcgccga ctttatcggc gatttcgcgg aattttgccc agtcgatttg
      781 caacgcgtag gcagacgcac ccgccacaat cattttgggt ttgtgttcga gcgcgaggcg
      841 ttcgacttcg gcataatcga gcacttcgtt ttcatccaaa ccataagtaa cagcgttgta
      901 gagtttgcct gagatattaa cgctcgcgcc gtgggtcagg tggccgccgt gcgccagaga
      961 catacccaaa atggtgtcgc ctggttttaa aacggaagcg tacacggctt ggttggcttg
     1021 cgagccggag tgcggttgga cgttggcgta ttgcgcgccg aacagttttt tgacgcggtc
     1081 aatcgccaat tgttcgacaa tatcgacgta ttcgcagccg ccgtagtagc gtttgccggg
     1141 gtagccttcg gcgtatttgt tggtcagctg ggaaccttgc gcttccatta cggcgcagct
     1201 gacgtagttt tcagaggcaa tcagctcgac gtggtcttgc tggcgttggt cttcttgggc
     1261 aatggctgct gccaaatcgg ggtcatattg tgcgagggta acgctttttg aaaacatgtt
     1321 ctcggctcct ttgtgtaatc agggtatcat gagtgttttt tgtataaaaa aatacttcaa
     1381 aacctaaggc agatagtcca taatgcgtaa attttctttg gcattatcag gtaatttatt
     1441 taacatgctg gtttttagcg tctcattacc tttatgttag tacaaggcta cccaaaggca
     1501 atgcatcaaa tggtaaattt tcggcagttt gtgcaaaatc aatcaaataa ccagcaccaa
     1561 ccacatcttt atcagccata acccgctgat acaccacttg ccaagacaac tctcctgccc
     1621 caccatgtct taaaatcaga tgaatggctt gtaatgctgt gtttggcgtc tcacaaattg
     1681 cgaagatctg ctcagaagct tgcttgatgg cttgtatttt tgtttgatta ctcacaattt
     1741 ccccctattt taatattaac ttaatgcggt caaattcaca aaatacaagc ttcacctcta
     1801 atcacccatc actcgacctt ctcggcgtgg atcggcacca ccaaccagcc tgcttggctc
     1861 gataataatg gcttgaacac ctgaatttag ctcacgcacg tcagtcttat agcccaaatc
     1921 atttaatgct tgttgccact ggagggcggt tgtacccgtt tctagttcat agttaccaaa
     1981 gcgatttaat aaattgggtg cactgatggc atcttggata tccatattcc agtcactatg
     2041 tgccacaatc gtcttagcga catagccaat gatacggcta ccacctgggg agccgattgc
     2101 catataaggc ttgcctgctt taaatacgat ggttggtgcc attgaggagc gtggtctctt
     2161 gccgggctcg acacgattgg cgacctgttt gccctgcttt attggctcaa aactaaagtc
     2221 tgtcagctca ttattcagca ggtagccatt tgccatcaaa gttgagccaa acgcattttc
     2281 aatggaagtc gtcattgata gcacattgcc cgccttatcc acaattgata tatgactggt
     2341 agaaggtaac tcaatcgctt gtgaggacac ccactcatga ataaaatcgc ctgcagatac
     2401 gctaggcaat gccttatccg actgctcaag cagctggctg cgatgtttta ggtagtcttt
     2461 agaaatcaac tggcgaatgg gtactggtac aaaatcaggg tcgcccaaat atacatcacg
     2521 atccgcaaac gcaagcctag aagcgtcgcc caagagacgt aaaccttcag catcataccc
     2581 cacctgattg ggtgaaaatt catttaaaat ccccaaaatt tgacccacag caatcccacc
     2641 tgagcttggt gcacccatac cgcatacttc ataaatacga taagtcacac aaacaggcgg
     2701 gcgttccacc acttgataat cagataaatc ttgtaaggat aattgaccgg ggttatcctt
     2761 agcattttgg acaactgaaa cgatattttg ggcatattta ccagtatgca gagcttttgc
     2821 accttgagct gctaacgcct gaacactgtc agcaaattct aaatttttca gcaagctgcc
     2881 tgcttgtagc ggcacaccat tcggcaaaaa ataagcggct gtttttggat agcgtgccaa
     2941 atgctgctga ttttgctcaa ccgagatggc aagccttggc gacacctcaa agccttgttt
     3001 tgccaagcgg atcggtgtat caaataattt tccccaaggc aatacaccgt atcgctgatg
     3061 tattgtctcc atcagtttag ggatagcagg cgtacccacc gagcgaccac cgaccaccgc
     3121 ttccataaat ttcaatggtt gaccatcttt atccaaaaat aattccggcg tcgcacgcat
     3181 cggtgccgtc tcacgcccat caaatgtggt caatgttttg gcggtattat cccaatacaa
     3241 cacaaatgca ccaccgccca agcctgacga ctgtggctct accaagctta gtgtcgtctg
     3301 caccgccacc atcgcatctg cagcgctacc gccttgcttt aagatatcat agccagcttg
     3361 tgttgctaat ggattggctg acgctaccat aaaatcactt gcaatcacct gcttttgttc
     3421 gatcagtccc gttgcatgtt caggcgtgtg agcgtctgca cctgtgatga cagcagaatg
     3481 agtattaacc ttaccttgat tggcatggat gacttgacat ccagagattg tcatagacat
     3541 tatcaatgca gtcaataaat atgttttagc cacaagcact ccttcgcctg agtttgattg
     3601 atgattcata caaggcatgc tgattattgt atttaatatg gctaaataat tcaatccaaa
     3661 ctatcaatct tgaccatcaa aaaaagaccg ctaatgtcat cagcagtctt ttttgatatt
     3721 tattttaaga tattaagtaa tcagaccttt gggctatgct cttcaatgag tggttttagc
     3781 tcacctgatt ggtacatttg taggataata tcactaccac cgattaactc accattaacc
     3841 caaagctgtg gaaaggttgg ccgattggcg atgagtggta gagtactgcg aatttctggg
     3901 ttttctagga tattaacaaa agcaaagggt ctgccaattg ggtcagcacc tctactgcac
     3961 gcgctgaaaa tccacattgg ggaaactggg gcgtgccttt
//
LOCUS       DAXHSF010000164         3859 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.164, whole genome shotgun sequence.
ACCESSION   DAXHSF010000164 DAXHSF010000000
VERSION     DAXHSF010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.164"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            490..1446
                     /gene="thiL"
                     /locus_tag="ACFM8S_001803"
     CDS             490..1446
                     /gene="thiL"
                     /locus_tag="ACFM8S_001803"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFC6314833.1"
                     /translation="MKEFDFIKRYLQTGTDNDVVLGIGDDAAIVRPREGFDLCFSADM
                     LLKDRHFFANVKPEDLAWKVLAVNVSDMAAMGAIPRWVLLSAALPELDEVWLKRFCGS
                     FFGLAKKFGVTLIGGDTTKGDMAFNVTIIGELPKGRALRRDAAVAGDDIWVSGRVGMA
                     AAALNCRLKRCVLPDDVFAECEQKLLRPEPRVGLGLALLPFARAAQDVSDGLAQDLGH
                     ILTASGVGAEIWADSLPSLSVLKDILPRAQWLSYTLAGGDDYELVFTAPESCRSRVLD
                     AAERCGVPVTRIGKINGGCRLKVLDADGRELELHSLGFDHFG"
     gene            1439..1924
                     /locus_tag="ACFM8S_001804"
     CDS             1439..1924
                     /locus_tag="ACFM8S_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFC6314834.1"
                     /translation="MADFKPDFAWLLKRPLCFLAFGFGSGLAPFAPGTFGTLAALPLA
                     FVLILLGIDGLLLAFLCIVLFMWGIRICAYAERETGVSDHGGIVWDEIVAMLFVLAFV
                     PFRWTWWLAAFVLFRLFDALKPSPVGWFDKNLHGGLGIMADDMAAAVMTLIVLRIAML
                     F"
     gene            complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="ACFM8S_001805"
     CDS             complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="ACFM8S_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFC6314835.1"
                     /translation="MSQQYVYSMLRVSKVVPPQKTIIKDISLSFFPGAKIGLLGLNGA
                     GKSTVLRIMAGVDKEFEGEAVPMGGIKIGYLPQEPELDPEKTVREEVESGLGEVAAAQ
                     KRLEEVYAEYANPDADFDALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPEWD
                     AKIDNLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAV
                     THDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQEL
                     EWVRQNAKGRQAKSKARLARFEEMSNYEYQKRNETQEIFIPVAERLGNEVIEFVNVSK
                     SFGDKVLIDDLSFKVPAGAIVGIIGPNGAGKSTLFKMIAGKEQPDSGEVKIGQTVKMS
                     LIDQSREGLQNDKTVFDNIAEGRDILQVGQFEIPARQYLGRFNFKGSDQSKITGQLSG
                     GERGRLHLAKTLLGGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLD
                     RIATHILACEGDSKWVFFDGNYQEYEADKKRRLGEEGAKPKRIKYKPVTR"
BASE COUNT          784 a          943 c         1077 g         1055 t
ORIGIN      
        1 aaggcaacgc tgtactggtt taaatttaat ccactatatc ggttgaaact ctgattttaa
       61 ggcggtagga tgtgggtttg cccatagaaa gggaatcctt tctgtatcaa gccctgaaag
      121 ggataattca tacaaattca cgcctttccc cttcattggg aaatggatgg aatcgtgcca
      181 gatgtgtgcg gcactgtatg ccggatatgg ttttatcatc agcccttttc ggttgaaacc
      241 ccgtcagttg cagcgattga gcctaatcgg tggcggaagt tgccgctttg cattcggggt
      301 ggcgtgcagt gcggtgcttt gatatgccgt ttgtgtgttg aaacagggtg gtcggtgcat
      361 acgggtacgg tatggccaaa gctaaaagtg aaatacgctg aaacactgaa tgagccgctt
      421 tattgtttgt acggcctttg ctgccttgct atgatttaaa ttggattcgc ccgccggata
      481 ttttgggata tgaaagaatt tgacttcatc aaacggtatt tgcaaacagg cacggataat
      541 gatgtcgtat tgggcatagg cgacgatgcg gcgattgtcc gcccgcgtga aggcttcgat
      601 ttgtgtttca gtgcggatat gcttttgaag gacaggcatt tttttgcaaa tgtcaaacct
      661 gaagacttgg cttggaaggt tttggccgtc aatgtttcgg atatggcggc gatgggtgcg
      721 ataccgcgtt gggtgttgct gagcgcggcg ttgcccgaat tggatgaggt atggctgaaa
      781 cggttttgcg gcagcttttt cggtttggca aaaaagtttg gcgtaacgtt aatcggcggc
      841 gatacgacca agggcgatat ggcgttcaat gtaaccatta tcggcgaatt gccgaagggt
      901 agggcgttgc ggcgtgatgc ggcggttgcg ggcgacgata tttgggtgtc ggggcgtgtc
      961 ggtatggcgg cggcggcttt gaactgccgt ctgaaacggt gtgtgttgcc ggatgatgtg
     1021 tttgctgaat gcgaacaaaa gctgctccgc cccgagccaa gggttgggct ggggcttgcg
     1081 ctgttgccgt ttgcccgggc ggcgcaggat gtttcagacg gcctcgcgca agatttgggg
     1141 catatcctga ccgcttccgg cgtgggggcg gaaatttggg ccgattcgct gccgtcttta
     1201 tccgtattga aagatatttt gccccgagcg caatggctgt cttatacttt ggcgggcggc
     1261 gacgattacg agctggtgtt taccgcgccg gaaagttgcc gcagccgcgt gcttgatgcg
     1321 gcagaacggt gcggcgtgcc ggtaacgcgc atcggcaaaa tcaacggagg atgccgtctg
     1381 aaggttttag atgccgacgg cagggaattg gaactacatt ctttaggatt cgatcatttt
     1441 ggctgatttt aaacctgact ttgcgtggct gttgaaacga ccgttgtgtt ttttggcttt
     1501 cggtttcggc agcgggctgg ctccgttcgc gccgggtaca tttggcactt tggcggcact
     1561 gcctttggca tttgtgctga ttttgctcgg catagacggg ctgctgctgg cttttttgtg
     1621 tatcgtgctg tttatgtggg gcatacgcat ttgcgcttat gcggaacgtg aaacgggtgt
     1681 cagcgaccat ggtgggattg tttgggacga gattgtcgcc atgctgtttg tgctggcgtt
     1741 tgtgccgttc aggtggacgt ggtggctggc ggcatttgtc ctattccgtc tgtttgacgc
     1801 gctcaaaccg tctcccgtcg gttggtttga caagaatctg cacggcggtt tgggcattat
     1861 ggcggacgat atggcggctg cggtgatgac tttgattgtc ttgaggattg caatgctgtt
     1921 ttaaacggtg ctgccttgta aaaatgccgc ctgaaagcct ttcagacggc attgtttcgg
     1981 aggttaacgt gttaccggtt tatatttaat ccgtttcggt ttcgcgcctt cttcgccgag
     2041 tcggcgtttc ttatcggctt cgtattcctg atagttgccg tcaaagaaca cccatttgga
     2101 gtcgccttcg caagccaaga tatgcgtagc aatacggtcg aggaaccagc ggtcgtgcga
     2161 aatcaccatc acgctgccgg caaattccag caatgcgtct tccaacgcgc gcagggtttc
     2221 cacgtcgagg tcgttggacg gttcgtccag cagcaacaca ttgccaccgc ccaacaaggt
     2281 ttttgccaag tgcaaacgtc cgcgttcgcc gccggaaagc tgccccgtga ttttgctttg
     2341 gtcgctgcct ttgaaattga agcgtcccaa atattggcgg gcggggattt caaactgccc
     2401 gacctgtaaa atatcgcgac cttcggcaat gttgtcgaac acggttttgt cgttttgcaa
     2461 accttcgcgg ctttggtcaa tcaagctcat tttcacggtt tgcccgattt tcacttcacc
     2521 ggaatcgggc tgctctttgc ccgcaatcat tttaaacagt gtcgatttac ccgcgccgtt
     2581 cggaccgatg atgccgacaa tcgcgcccgc aggcactttg aagctcaaat cgtcaatcag
     2641 cactttgtcg ccgaacgatt tggaaacatt cacaaattca atcacttcgt tacccaaacg
     2701 ctcggcgacg ggaatgaaga tttcctgcgt ttcattgcgt ttttggtatt cgtagttgct
     2761 catttcttca aaacgagcca aacgcgcttt ggacttggct tggcggcctt tggcattttg
     2821 gcgcacccat tccaattcct gcttcatcgc cttcacgcgc gcggcttcgg attttgcctc
     2881 gttttccaag cgtttttctt tctgctccag ccaagacgag taattgcctt tccacggaat
     2941 accatggccg cggtcgagtt ccaaaatcca ttcggcggcg ttgtcgagga agtagcggtc
     3001 gtgtgttacc gcaacgactg taccggggaa gcgcacgaga aattgctcca gccactcgac
     3061 agattccgcg tccaagtggt tggtcggctc gtccagcaaa agcatatcgg gtttgctcaa
     3121 caagagtttg cacaaggcaa cgcggcgttt ttcaccgccg gacaaattat cgattttggc
     3181 atcccattcc ggcaggcgca gcgcgtcggc ggcgatttcc aattcgtgtt ccgcaccgcc
     3241 gcccgtggac gaacctgccg caataatcgc ttccaagcgg ccctgctctt ctgccaacgc
     3301 gtcaaaatcc gcatcaggat tggcgtactc ggcatacact tcttccaaac gtttctgcgc
     3361 ggcagccact tcgcccaaac cgctttccac ttcctcgcgc acggtttttt caggatcaag
     3421 ctcaggctct tgcggcaggt agccgatttt aatgccgccc atcggcacgg cttcgccctc
     3481 aaattcctta tccacacccg ccataatccg cagcacggtg gacttgcccg cgccgttcaa
     3541 accgagcagg ccgattttcg cgccggggaa gaaagaaagg gaaatatctt taatgatggt
     3601 tttctgcggc ggcacaacct tgctcacgcg cagcatagaa tagacgtatt gttgggacat
     3661 ggttttctcg ttttcatcaa acaaatttca gacggccatt ttaaccgata atttgattta
     3721 agccagttta tccgcgaacc ggtattgcca aaatcgggca ggattcataa aatccgctta
     3781 tccctttgaa attatataga caaaaaaata ataatgatag gggatcgccg ccccggcaac
     3841 catttcggat tttccaaag
//
LOCUS       DAXHSF010000165         3853 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.165, whole genome shotgun sequence.
ACCESSION   DAXHSF010000165 DAXHSF010000000
VERSION     DAXHSF010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.165"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            94..321
                     /locus_tag="ACFM8S_001806"
     CDS             94..321
                     /locus_tag="ACFM8S_001806"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314836.1"
                     /translation="MFGFRLSGGKGILQRSRALIAAKIDQAEISDSEKSWLKKELGKI
                     KDTALSTLTENAINAIPAATLITLLKKFVGL"
     gene            complement(318..824)
                     /locus_tag="ACFM8S_001807"
     CDS             complement(318..824)
                     /locus_tag="ACFM8S_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HFC6314837.1"
                     /translation="MAHPQEIREKLRRLYVSGEQTLETAALMCEIPQTTARAWKRADK
                     EKGDDWDKMRAAYTLAGGGIEDLSRAMLAGFMVQYNSTMTMLQDSSTEDLPPSDRAKL
                     LASLTDAFTKTVSANARVMPETSKLATALELIEFLMAFVQEKHPKHLPAFVEVLEPFG
                     VEVEKKFG"
     gene            complement(827..1024)
                     /locus_tag="ACFM8S_001808"
     CDS             complement(827..1024)
                     /locus_tag="ACFM8S_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314838.1"
                     /translation="MSDPILEALARIENKTDQTLKNQKEMQAEIAQIRQDTKRTAITF
                     GALGGGVITVGWELLKAKMGL"
     gene            complement(1021..1287)
                     /locus_tag="ACFM8S_001809"
     CDS             complement(1021..1287)
                     /locus_tag="ACFM8S_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314839.1"
                     /translation="MDFEFGFRTLWPIATAAFWFWVNGISGRLKEADKRIDDLKEELH
                     AVKLSYHTKQDAKADRDNIAASLGRIENKLEKVNEKLDRKADKS"
     gene            complement(1299..1637)
                     /locus_tag="ACFM8S_001810"
     CDS             complement(1299..1637)
                     /locus_tag="ACFM8S_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314840.1"
                     /translation="MTYRELVERQLAVRHADLELGLSRAREQEPFVIHVSDLLDKAGI
                     EYAVRMDKDFQTTFHLEYPITNYDTFKRAVWQTLGAYYCVCNDGDGLEIASNRPDGYA
                     VRIVFGDVPV"
     gene            complement(1634..1852)
                     /locus_tag="ACFM8S_001811"
     CDS             complement(1634..1852)
                     /locus_tag="ACFM8S_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314841.1"
                     /translation="MNEYTFSYRFNGKSWSLSIWADNPEEARAKFRAARENAHYDGEV
                     VAKVYTFVNISWVKKLYKRTKYLMGIKE"
     gene            1865..2035
                     /locus_tag="ACFM8S_001812"
     CDS             1865..2035
                     /locus_tag="ACFM8S_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFC6314842.1"
                     /translation="MYFEIYKDVKGEYRWRLKAANHEIIAQGEGYTSKQNCQHAVDLL
                     KSTTAATPVKEV"
     gene            complement(2080..2385)
                     /locus_tag="ACFM8S_001813"
     CDS             complement(2080..2385)
                     /locus_tag="ACFM8S_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314843.1"
                     /translation="MSLLKTVKMQAAVVLTALAITACSSTTAPSATEIKVVEKAVLPT
                     PPAALMVAPVRPNPPKDGKTATLLEHAAEFGGYVAELENQNQAWRDWAGNHSRKVGN"
     gene            complement(2282..2701)
                     /locus_tag="ACFM8S_001814"
     CDS             complement(2282..2701)
                     /locus_tag="ACFM8S_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314844.1"
                     /translation="MNLVKLLANNWQPIAIIALVGTLLAVSHHQGYKSAFAKQQAVID
                     KMEKDKAQALLLSAQNYARELEQARAEAKKYEVKAHAVGMALAKKQAEVSRLKTENKK
                     EIENVLTQDRKNAGGGCIDGFGHHGLQLYNRALGYGN"
     gene            complement(2673..2909)
                     /locus_tag="ACFM8S_001815"
     CDS             complement(2673..2909)
                     /locus_tag="ACFM8S_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314845.1"
                     /translation="MRILDIFKNPATGNVSHSKLWANVACAAGTVKFVMLPDPSAEIW
                     AVYLGIVGGYAVARSLVSVKRQEVENESRETAGE"
     gene            complement(2912..3076)
                     /locus_tag="ACFM8S_001816"
     CDS             complement(2912..3076)
                     /locus_tag="ACFM8S_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314846.1"
                     /translation="MGQVAFYEKMIGLWSAKSREASEQADLAAFEFAEGELANYQEML
                     KRHLQTKSVE"
     gene            complement(3079..3285)
                     /locus_tag="ACFM8S_001817"
     CDS             complement(3079..3285)
                     /locus_tag="ACFM8S_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314847.1"
                     /translation="MNIIGKLKEAASYFLTKLIGENPSNEQVNRALIQMPNVRPIHTY
                     PRPNLRNSGVAAAKRAARKRKNRR"
     gene            complement(3282..3827)
                     /locus_tag="ACFM8S_001818"
     CDS             complement(3282..3827)
                     /locus_tag="ACFM8S_001818"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFC6314848.1"
                     /translation="MGKTVTLTAGHSNTDPGAVNGSDREADLAQDMRNIVASILRNNY
                     GLTVKTDGTGKGNMPLREAVKLIRGSDVAIEFHTNAAVSKAATGIEALSTVKNKRWCQ
                     VLSKAVAKKTGWKLRGEDGFKPDNAGQHSRLAYAQAGGIVFEPFFISNDTDLALFKTT
                     KWGICRAIADAIAMELGAARV"
BASE COUNT          877 a         1082 c          889 g         1005 t
ORIGIN      
        1 gaccggatgc ctgattatcg ggtatccggg gaggattaag ggggtatttg ggtagaatta
       61 ggcggtattt ggggcgaaaa cagctgaaaa cctgtgtttg ggtttcggct gtcgggaggg
      121 aaaggaattt tgcaaaggtc tcgggcttta attgcggcaa aaatagacca agcggagatt
      181 tctgattcag aaaaaagctg gctgaaaaaa gaattaggga aaatcaagga cactgcatta
      241 agtacgctta cggaaaatgc aattaatgct atccctgccg ccaccctcat tactctttta
      301 aaaaaatttg tcggcctcta accaaacttc ttctccactt ccaccccaaa cggctccaat
      361 acctccacaa aggcaggcaa atgtttggga tgtttttctt gcacaaacgc catcaagaac
      421 tcaatcaatt ccaaagccgt cgccagtttt gacgtttccg gcatcacacg cgcattggcg
      481 gatacggttt tcgtaaacgc atcggtcagg ctggccaaca gcttggcgcg gtccgatggc
      541 ggcaaatctt cggtactcga atcctgcagc atcgtcatcg tgctgttgta ctgcaccata
      601 aaaccggcca acatcgcacg gctcaagtcc tcaataccgc cgccggccaa agtgtaggcg
      661 gcgcgcatct tatcccagtc gtcgcctttt tccttatccg cacgtttcca cgcacgcgca
      721 gtggtctgcg ggatttcgca catcaaggcc gccgtttcca aagtttgctc gccgctcaca
      781 tagagccggc gtaacttttc acggatttct tgcgggtgag ccataattac agtcccattt
      841 tcgctttaag caattcccag ccgaccgtaa tcacgccgcc gcccagtgcg ccgaatgtaa
      901 tggccgtgcg tttcgtgtct tggcggattt gcgcaatttc cgcctgcatt tctttctgat
      961 ttttcagcgt ttgatcagtc ttgttttcaa tacgcgccaa ggcttctaaa atcgggtcgc
     1021 tcatgatttg tctgctttcc tgtccagttt ttcgtttact ttttccaact tgttttcgat
     1081 gcgtcccaac gaagccgcaa tattgtcgcg gtctgccttg gcatcttgct tggtgtgata
     1141 agagagcttg accgcgtgca gctcctcttt caggtcgtcg atacgcttgt ccgcttcttt
     1201 cagacggcct gaaataccgt tgacccaaaa ccaaaatgcc gccgtcgcaa tcggccacag
     1261 ggttctgaaa ccaaattcaa agtccattta aaaccccttt aaaccggcac atcgccgaat
     1321 acgatacgga cggcgtaacc gtcagggcga ttgctggcaa tctccagtcc atcaccatca
     1381 ttacaaacac agtaatacgc ccccaaagtt tgccaaaccg cacgtttaaa ggtgtcatag
     1441 ttcgtaattg gatattcaag gtgaaacgtc gtctgaaaat ccttatccat gcgtaccgcg
     1501 tactcaatgc ctgccttatc caacagatcg gaaacatgaa tgacaaacgg ctcttgttcg
     1561 cgtgcgcggc ttaagcccaa ttccaaatcg gcatggcgca cagccaactg acgttcaact
     1621 aattcacggt aggtcattct ttgataccca ttaaatattt tgtccgcttg tacaatttct
     1681 taacccacga aatatttaca aatgtataaa cctttgctac aacttcgccg tcatagtgcg
     1741 cattttctcg tgcagcccga aatttcgccc tggcttcttc agggttgtcc gcccaaatgc
     1801 tcaatgacca ggacttgccg ttaaagcggt aagaaaacgt gtactcattc ataggagaaa
     1861 ccttatgtat tttgaaatct ataaagacgt aaaaggcgaa taccgttggc gtttgaaagc
     1921 agccaaccat gaaatcatcg ctcagggcga aggctacacc agcaagcaaa actgtcagca
     1981 cgcagtcgat ttgctgaaaa gcactaccgc cgctacccct gtaaaagagg tataaaatcc
     2041 gctttcaccc tcagcccgcg ccctacgcgg gcttttttgt cagtttccga ctttgcggga
     2101 gtgattgccc gcccagtcgc gccaagcctg attttgattt tccaactccg ccacatagcc
     2161 gccaaactcg gcggcgtgtt ccaacagcgt ggccgtcttg ccgtctttcg gcggattcgg
     2221 gcgcaccggc gcgaccatca acgcggcagg cggtgtcggc aagaccgcct tttcgacaac
     2281 cttaatttcc gtagccgagg gcgcggttgt agagctgcag gccgtgatgg ccaaagccgt
     2341 caatacaacc gccgcctgca tttttacggt cttgagtaag gacattttcg atttcctttt
     2401 tattttccgt tttcagacgg ctgacttccg cctgtttttt cgccaaagcc atgccgacag
     2461 cgtgcgcctt gacttcatat tttttagctt ccgcacgcgc ctgttccagc tcgcgggcgt
     2521 agttttgagc cgacaacagc agggcttgcg ctttgtcttt ttccatcttg tcaatgaccg
     2581 cctgctgctt cgcaaaagcc gacttgtagc cttgatggtg cgacaccgcc aagagcgtgc
     2641 cgacaagcgc gatgatggca atcggttgcc agttattcgc cagcagtttc acgagattca
     2701 ttctcgacct cctgacgttt gacgctgacc aacgaacgcg ccaccgcata gccgccgaca
     2761 atgcccaaat acaccgccca aatctccgcc gacgggtcgg gcagcatcac aaacttaacc
     2821 gtccccgccg cgcaggcaac gtttgcccac agtttcgagt gcgacacatt gcctgtcgct
     2881 gggtttttaa aaatatccaa aatacgcatt gctattccac acttttggtt tgcaggtgcc
     2941 gtttcagcat ttcctgataa ttggccagtt cgccctccgc aaattcaaac gccgccaagt
     3001 ctgcctgttc gcttgcctca cggcttttgg ccgaccacag cccaatcatc ttttcgtaaa
     3061 acgcaacctg tcccatgatt aacgacgatt cttgcgtttg cgcgccgcgc gtttcgcggc
     3121 tgccacgcct gagtttctta aatttgggcg tggataggtg tgtatcggac gaacatttgg
     3181 catctgtata agtgcgcggt ttacctgctc attactagga ttttctccaa tcaattttgt
     3241 aagaaaatag gaagcagctt ctttcaattt accaataata ttcatactct tgccgccccc
     3301 aattccatcg caatcgcgtc cgcaatcgcg cggcagatgc cccatttagt cgtcttaaac
     3361 aaggccaaat cagtgtcgtt gctgatgaaa aaaggctcaa acacaatgcc gccggcttgt
     3421 gcataagcca ggcgcgaatg ctggcccgca ttgtcgggtt taaagccgtc ttcgccgcgc
     3481 agtttccagc cggttttctt ggcaacggct ttgctcaaca cctgacacca gcgtttgttt
     3541 ttaacggtac tcaaggcttc gatgcctgtc gccgctttgc tgacggcagc gttggtgtga
     3601 aactcaatcg ccacatccga gccgcgaatc agcttgaccg cttcgcgcag cggcatattg
     3661 cctttgcccg tgccgtcggt tttaacggtc aggccgtaat tgttacgcag gattgaagcc
     3721 acaatgttgc gcatatcctg cgccaagtcc gcctcacggt cgctgccgtt gaccgcaccc
     3781 gggtcggtgt tgctgtgtcc agcggttaag gttacggttt tgcccattca tcatctccaa
     3841 aaaatattga ttg
//
LOCUS       DAXHSF010000166         3844 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.166, whole genome shotgun sequence.
ACCESSION   DAXHSF010000166 DAXHSF010000000
VERSION     DAXHSF010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.166"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            94..1118
                     /gene="hupA"
                     /locus_tag="ACFM8S_001819"
                     /pseudo
     CDS             94..1118
                     /gene="hupA"
                     /locus_tag="ACFM8S_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225846.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupA"
     gene            1149..3578
                     /gene="hupB"
                     /locus_tag="ACFM8S_001820"
     CDS             1149..3578
                     /gene="hupB"
                     /locus_tag="ACFM8S_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupB"
                     /protein_id="HFC6314849.1"
                     /translation="MPIPFKPVLAAAAIAQAFPAFAADPAPQSAQTLNEITVTGTHKT
                     QKLGEEKIRRKTLDKLLVNDEHDLVRYDPGISVVEGGRAGSNGFTIRGVDKDRVAINV
                     DGLAQAESRSSEAFQELFGAYGNFNANRNTSEPENFSEVTITKGADSLKSGSGALGGA
                     VNYQTKSASDYVSEDKPYHLGIKGGSVGKNSQKFSSITAAGRLFGLDALLVYTRRFGK
                     ETKNRSTEGDVKIENDGYVFDPTNPSPSRYLTYKATGVARSQPDPQEWVNKSTLFKLG
                     YNFNDRNRIGWIFEDSRTDRFTNELSNLWTGTIYSPATGDYRHRQDVSYRRRSGVEYK
                     NELEHGPWDSLKLRYDKQRIDMNTWTWDIPKNYDLRGINSEVYHSFRHIRQNTAQWTA
                     DFEKQLDFSKAVWAAQYGLGGGRGDNANSDYSYFAKLYDPKILTSNQAKITMLIENRS
                     KYKFAYWNNVFHLGGNDRFRLNAGIRYDKNSSSAKDNPKYTTAIRGQIPHLGSERAHA
                     GFSYGTGFDWRFTKHLHLLAKYSTGFRAPTSDETWLLFPHPDFYLKANPELKAEKAKN
                     WELGLAGSGKAGNFKLSGFKTKYRDFIELTYMGVSSDDKNNPRYAPLSDGTALVSSPV
                     WQNQNRTAAWVKGIEFNGTWNLDSIGLPKGLHTGLNVSYIKGKATQNNGKETPINALS
                     PWTAVYSLGYDAPSKRWGINAYATRTAAKKPSDTVHSNDDLNNPWPYAKHSKAYTLFD
                     LSAYLNIGKQVTLRAAAYNITNKQYYTWESLRSIREFGTVNRVDNKTHAGIQRFTSPG
                     RSYNFTIEAKF"
BASE COUNT         1062 a         1194 c          874 g          714 t
ORIGIN      
        1 tttttgcatc gacccacaaa acagttgaca tcatataaca tttaaactga taatcaaaac
       61 tattattaac acatcatcaa gattgagtat gcgatgaaat acaaagccct gtccttactg
      121 ccgcttgtcg ctgcccttgc cgcctgtgcg gggggggggt agccgaaccg cacgtccccg
      181 tgtccatccc caccgccacg ccgctgaccg gtgaggtaaa gttaacagac gacaatagca
      241 aaatcgaaaa catcaacacc gccaacactg gaaacacatc cggcatttcc attcagcagc
      301 gggaatataa agtaaacaat tacggcgtcg aatcaacagc aaaagcattc atcttcaaaa
      361 cacctggcgg cgcgcaatac accctttcat cctacgccga ccccatcgtc cccagctact
      421 cctcccccga tttcaaaatt cccgaccgcc acgcagggca gcggcttgcc gacggcagcc
      481 gcatctttat ctgctgcagc gactccgggg caaccaatca agcggaaatt accaaacaag
      541 attatatgaa attcggggcg tggataggtc ccaacggcga aatcgacctc ttcgccggcg
      601 gcttccccgt cggcaaaaca ccggcatcaa gcagttatta cggcagcagc acgcccgaaa
      661 cccaaggcaa gggcaaaatc acttatcagg tttggggcat ccgcgtcaga aacgggcaat
      721 ttgttacttc ctcttatacg ccgccgaaaa acagtggtta ttattcgtcc acacctacca
      781 acacccccgt cctttccttt atcaccgcca atttcaacac ccataaactg tcaggcgaaa
      841 tcatcggcaa cagcgattac gggccaaatg tgaaaattga aaacgcaacg attgacggac
      901 tatccttttc cggcgatgcc acgtccggcg gcaaaaacgg caaattggaa ggcaagtttt
      961 ttggcaagtt caacagcacc cgcagcagcg acaccggcat aggcggcaaa atcactttta
     1021 aagacgaccg ctccctcgat accgtattcg gcggcgtgat ttatgagaaa aaacttgatg
     1081 acaaaaccag tcaagatacc aaccatctca aaaaataata acccatccgc ttttaaaaag
     1141 gttttcttat gcccatcccc tttaaacccg tattggctgc cgccgccatc gcccaagcgt
     1201 ttcccgcctt tgcggcagac cccgcgccgc agtccgccca aacgctgaac gaaatcaccg
     1261 ttaccggcac gcacaaaacc caaaaactcg gcgaagaaaa aatccgccgc aaaactttag
     1321 acaagctctt ggtcaacgac gaacacgacc tggtgcgcta cgaccccggc atttccgtcg
     1381 tcgaaggcgg cagggcgggt tctaacggct ttaccatacg cggcgtggac aaagaccgcg
     1441 tcgccatcaa cgttgacggg ctggcgcagg cggaaagccg ctcttccgaa gccttccaag
     1501 agttgttcgg cgcgtacggc aacttcaacg ccaaccgcaa cacctccgag ccggaaaact
     1561 tttccgaagt aaccatcacc aaaggcgcgg actcgcttaa atccggcagc ggcgcattgg
     1621 gcggcgcagt caattaccaa accaaatccg caagcgatta tgtttccgaa gacaagccct
     1681 accatttggg gataaagggc ggcagcgtcg gcaaaaacag ccaaaaattc agcagcatca
     1741 ccgccgccgg caggctcttt ggtttggatg ccttattggt ttatacccgc cgcttcggca
     1801 aagaaaccaa aaaccgctcg accgagggcg atgtcaaaat tgaaaacgac ggatatgtct
     1861 ttgacccaac caacccctcg cccagcagat accttaccta caaagccaca ggggttgcgc
     1921 gctcccaacc cgacccgcaa gaatgggtaa acaaaagcac cctgttcaag ctgggctaca
     1981 atttcaacga ccgcaaccgc atcggctgga tttttgaaga ctcccgcacc gaccgtttta
     2041 ccaacgagct gtctaatttg tggacgggta caatttactc tcccgcaacg ggcgactacc
     2101 gccaccgcca agacgtgagc taccgccgcc gctccggcgt cgaatacaaa aacgaattgg
     2161 aacacggccc gtgggacagc ctcaagctgc gctacgacaa gcagcgcatc gatatgaaca
     2221 cttggacttg ggacatcccg aaaaattacg atttgcgcgg catcaacagt gaagtttacc
     2281 attcgttccg gcatatccgc caaaacaccg cgcaatggac tgccgatttt gaaaaacaac
     2341 tcgacttttc caaagccgtt tgggcggcgc aatacggctt gggcggcggc agaggggaca
     2401 atgccaactc ggattacagc tatttcgcaa aactgtacga ccccaaaatc ctcacttcca
     2461 accaagccaa aatcacaatg ctgatcgaaa accggtcgaa atataaattt gcctattgga
     2521 acaatgtgtt tcacttgggc ggcaacgacc gcttccgcct gaatgcgggc atacgctacg
     2581 acaaaaacag cagcagcgcg aaagacaatc cgaaatacac caccgccatc cgggggcaga
     2641 ttccccattt gggttcggaa cgcgcgcacg cgggcttcag ctacggcacg gggttcgact
     2701 ggcggtttac caagcatctg cacttgttgg caaaatacag caccggcttc cgcgcgccga
     2761 cttcggacga aacctggctg ctgttcccac accccgattt ctacctgaaa gccaaccccg
     2821 aactgaaagc ggaaaaagcc aaaaactggg aattgggtct ggcgggcagc ggcaaggcgg
     2881 gcaacttcaa actctcaggc ttcaaaacca aataccgcga ctttatcgaa ctgacgtata
     2941 tgggcgtttc gtcagacgat aaaaacaacc ccagatacgc cccgctttcc gacggtactg
     3001 cattggtcag ctcgcccgtt tggcaaaacc aaaaccgcac cgccgcctgg gtgaaaggca
     3061 tagagtttaa cggcacttgg aacctcgaca gcatcgggct gcccaaaggg ctgcacaccg
     3121 gcctcaacgt cagctacatc aagggcaagg caacgcaaaa caacggcaaa gaaacgccca
     3181 tcaacgcgct ttcgccgtgg acggcggttt acagcctggg ctatgacgcg ccttccaaac
     3241 gctggggcat caacgcctac gccacacgca ccgccgccaa aaagccgtcc gacaccgtac
     3301 acagcaacga cgacttgaac aacccgtggc cttatgccaa acacagcaag gcctatacgc
     3361 tgttcgacct ttccgcctac ctcaacatcg gcaaacaggt tacgctccgc gccgccgcgt
     3421 acaacattac caacaagcag tactacactt gggaatcttt acgcagcatc cgcgaattcg
     3481 gcacagtcaa ccgcgttgac aacaaaaccc acgccggcat ccaacgcttt acctcgccgg
     3541 gcaggagcta caatttcacc atcgaagcga agttctaagc tcctgatgtg ccaatgccgt
     3601 ctgaaacccg atgatttggg tttcagacgg cattttttac ggtcgcaccg ccgccccctt
     3661 tcaggcttgt accaagccca atcccgcaac tctccgtcat tcccgcgact ctccgtcatt
     3721 cccacggaag tgggaatcta gaaacgcaaa gctgcaagaa tttatcggaa atgactgaaa
     3781 ctcaacgaac ctggattccc gcctgcgcgg gaatgacgaa gctatccgca cagaaacctg
     3841 cacc
//
LOCUS       DAXHSF010000167         3837 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.167, whole genome shotgun sequence.
ACCESSION   DAXHSF010000167 DAXHSF010000000
VERSION     DAXHSF010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.167"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            234..2114
                     /locus_tag="ACFM8S_001821"
     CDS             234..2114
                     /locus_tag="ACFM8S_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HFC6314850.1"
                     /translation="MPYFALFDDAVSGRAKRYQNYVESRFFRPEELDALDGALQKGWQ
                     KGLHSVLFADYEFGLPLTGVESERGGNLALHWFADCTDTDAASWLARHSDDLPAGIST
                     PQSSVSETDYLDRIRQIHEAIRRGDTYQINYTTRLHLQAYGNPVSLYRRLRQPVPYAV
                     LSHLPDAEGQSAWTLCFSPELFLKIGSDGTISTEPMKGTAPILGDGQDERRAAELQAD
                     PKNRAENVMIVDLLRNDLGKIAQTGKVCVPEPFKVSRFGSVWQMTSTIQAQALPHTSF
                     ADILRAAFPCGSITGAPKKMSMQIIESLETEARGLYTGSIGYLNPCSGGLGFEGTFNV
                     VIRTLSLKPVSASDGIVSGIGGPDSNAQARTAGQGGATPHPFDSNPPYRGVYGVGSGI
                     VIDSDPAAEYRECGWKARFLNELRPDFGIFETLRAENGRCTLLDRHLCRLKTSARALN
                     LPLPDGCENQIKQYIADLPDGVFRIKALLASDGISLSRAVLNRLTDKQRVIISPAVLP
                     AQNYLRHFKTTHRALFDQAWQTAETQGAFDSLFFNSDGILLEGGRSNVFVKYQGQWLT
                     PSLDLDILNGIMRQAVLDEPQKYLQTNQVIETHITQKTLQEAEEIRLSNALRGVFAAA
                     LA"
     gene            complement(2173..3690)
                     /locus_tag="ACFM8S_001822"
     CDS             complement(2173..3690)
                     /locus_tag="ACFM8S_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC6314851.1"
                     /translation="MLYFRYGFLVVWCAAGVSAAYGADAPAILDDKALSQVWRSVSDK
                     WAESDWKVENDAPRVVDGDFLLAHPKMLEHSLRDALNGNQADLIASLADLYAKLPDYD
                     AVLYGRARALLAKLAGRPAEAVARYRELHGENAADERILLDLAAAEFDDFRLKSAERH
                     FAEAEKLDLPAPVLENVGRFRKKAEGLTGWRFSGGISPAVNRNANNAAPQYCWQNGGR
                     QICSVSRAERAAGLNYEIEAEKLTALADNHYLLFRSNIGGTSYYFSKKSAYDDGFGRA
                     YLGWQYKNARQTAGILPFYQVQLSGSDGFDAKTKRVNNRRLPPYMLAHGVGVQLSHTY
                     RPNPGWQFSVALEHYRQRYREQDRAEYNNGRQDGFYVSSAKRLGESATVFGGWQFVRF
                     VPKRETVGGAVNNAAYRRNGVYAGWAQEWRQLGGLNSRVSASYARRNYKGVAAFSTEA
                     QRNREWNVSLALSHDKLSYKGIVPALNYRFGRTESNVPYAKRRNSEVFVSADWRF"
BASE COUNT          907 a         1287 c          863 g          780 t
ORIGIN      
        1 tggagctgat cttgcttttc gcctaattct acgttttcaa acggttgcag ctggtggtct
       61 gccataaagg tctccttatt gtatttcagg ttggaaatcg gaatttgttt tcacaatttc
      121 acaccttcgc ccccgctttc tctacataaa attacatttt gccgatattt gccgaatcgt
      181 ctgaaaatat gtgtaaaaag agcgtataat caaaacattt gccccgaatt gccatgccct
      241 atttcgccct gtttgacgat gccgtaagcg gccgcgcaaa acgctatcaa aattatgtag
      301 aaagccgttt tttccgtccc gaagaactcg atgctttgga cggcgcgctg caaaagggct
      361 ggcaaaaagg gctgcattcg gtgttgtttg cagactacga attcggtttg ccgctgacgg
      421 gggttgagtc cgagcgcggc ggcaaccttg ccctgcactg gtttgccgac tgcaccgaca
      481 ccgatgccgc aagctggctt gcccgacact cagacgacct tcccgccggc atttccacgc
      541 cgcaatcctc cgtatccgaa accgattacc tcgaccgcat ccgccaaatc cacgaagcca
      601 tccggcgcgg cgacacctat caaatcaact acaccactcg cctgcacctg caagcctacg
      661 gcaatcccgt cagcctctac cgccgcctgc gccagcccgt cccctatgcc gtcttgtccc
      721 acctgcccga tgcggagggg caatccgcgt ggacgctgtg tttctcgccc gaactcttcc
      781 tcaaaatcgg ttcagacggc accatcagca ccgaaccgat gaaaggcacc gcgccgattt
      841 tgggcgacgg acaagacgaa cgccgcgccg ccgagttgca agccgacccg aaaaaccgcg
      901 ccgaaaacgt gatgattgtc gatttgctgc gcaacgacct cggcaaaatc gcccaaaccg
      961 gcaaagtatg cgtgcccgag ccgtttaaag tatcacgttt cggcagcgtt tggcagatga
     1021 ccagcaccat ccaagcccaa gccctgccgc acacctcgtt cgccgacatc ctccgcgccg
     1081 ccttcccctg cggcagcatc accggcgcgc ccaaaaaaat gagtatgcag attatcgaat
     1141 cgctcgaaac cgaagcacgc ggactttata cgggcagcat cggctatttg aacccgtgtt
     1201 ccggcggctt ggggtttgaa ggcacgttca acgtcgttat ccgcacctta tcgctcaaac
     1261 ccgtttcggc ttcagacggc attgtttcag gcatcggcgg cccggattca aacgcgcaag
     1321 cccgaacggc agggcaaggc ggggcaacgc cgcatccgtt tgactccaat ccgccgtatc
     1381 ggggcgtgta cggtgtcggt tccggcatcg tcatcgacag cgaccccgcc gccgaatatc
     1441 gcgaatgcgg ctggaaagcc cgtttcctca acgaattgcg ccccgacttc ggcatttttg
     1501 aaaccctgcg cgcggaaaac ggacgctgca ccctgctcga ccgccaccta tgccgtctga
     1561 aaacctccgc acgcgccctc aacctgcccc tgcccgacgg ttgcgaaaat caaatcaaac
     1621 aatacattgc cgacttgccc gacggcgtgt tccgcatcaa agccctgctc gcttcagacg
     1681 gcatcagcct gtcccgcgcc gttttaaacc gtctgaccga caaacaacgc gtcatcattt
     1741 cgcccgccgt cctgcccgca caaaactacc tgcgccactt caaaaccaca caccgcgccc
     1801 tcttcgacca agcgtggcaa accgccgaaa cacaaggcgc gttcgacagc ctgtttttca
     1861 actcggacgg cattctgctc gaaggcggca gaagcaacgt gttcgtcaaa taccaaggac
     1921 aatggcttac gccctcctta gatttagaca ttttaaacgg cataatgcgc caagccgtat
     1981 tggacgaacc gcaaaaatat ttgcaaacaa atcaagtaat cgaaacacac atcacacaaa
     2041 aaacactgca agaagccgaa gaaatccgcc tctccaacgc cctgcgcggc gtatttgccg
     2101 ccgcccttgc ctgaacgcgc aaaaatgccg tccgaacctg tttccaaagt tcggacggca
     2161 ttatcccacc attcaaaacc gccaatccgc cgacacaaac acctcgctgt tgcggcgttt
     2221 cgcatacggc acattacttt ccgtcctgcc gaaacgataa ttcaacgcgg gcacgatacc
     2281 tttgtacgac aacttgtcgt ggctcaaagc cagcgagaca ttccattcgc ggttgcgttg
     2341 cgcctctgtc gagaaagccg caacgccctt atagttgcgg cgggcataag acgcggaaac
     2401 ccgactattc aaaccgccca actgccgcca ctcctgcgcc caaccggcat aaacaccgtt
     2461 gcgccggtag gcggcattat tgaccgcgcc gcccaccgtt tcgcgtttcg gcacaaaccg
     2521 cacaaactgc cagccgccga acacagttgc cgattcgccc aaacgttttg ccgacgaaac
     2581 ataaaacccg tcctgcctgc cgttattgta ttccgcccta tcctgttcgc ggtagcgttg
     2641 gcggtaatgt tccagcgcga ccgaaaattg ccatcccggg tttggtcggt aagtatggga
     2701 cagctgcacg ccgactccgt gcgccagcat atacggcggc aggcggcggt tgtttacccg
     2761 ttttgttttc gcatcaaagc cgtcgctgcc cgacaactgc acctgataaa acggcaaaat
     2821 ccccgccgtc tgccgtgcat ttttatactg ccaacccaaa tacgccctgc cgaacccgtc
     2881 atcataagct gattttttac tgaaataata gctcgtgccg ccgatattgg aacggaacaa
     2941 caaataatga ttgtccgcca acgccgtcag cttttccgcc tcgatttcat aattcaaccc
     3001 tgccgcccgc tccgcccggc tgacactgca tatctgccgg cctccgtttt gccagcaata
     3061 ctgcggcgcg gcattattgg catttctatt gaccgccggg ctgataccgc ccgaaaaacg
     3121 ccagcccgtc agcccctccg cttttttccg aaaacgcccc acattttcca aaaccggcgc
     3181 cggcaaatcc aatttttccg cctcggcaaa atgcctttct gccgacttca gccggaaatc
     3241 gtcaaactcc gccgccgcca aatccagcaa aatccgctcg tctgccgcat tttccccgtg
     3301 cagttcccga taccgcgcca ccgcctccgc cggccttccc gccaatttcg ccagcaaagc
     3361 ccgcgccctg ccgtacaaaa ccgcgtcata atccggcagc ttggcataca aatccgccaa
     3421 cgaagcgatt aaatccgcct gattgccgtt gagcgcgtcg cgcaaactat gttccaacat
     3481 tttcgggtgc gccaacaaaa aatccccgtc aaccacgcgc ggggcatcat tttcaacttt
     3541 ccaatccgat tccgcccatt tatccgacac cgaccgccac acctgcgaca aagctttgtc
     3601 atccaaaatt gcaggcgcat ccgccccata ggcggcagaa acacctgccg cacaccaaac
     3661 aaccaaaaaa ccgtatctga aatacaacat accctgtcat ttacctttcc ggcaaacacg
     3721 ccgccgaagc acgtcaaacc atccgaaaaa caggcaaaag cccgtaaaaa ccggctttgc
     3781 cgcctgaaag cgggcaggca aaaaaccgcc gcccgatttt caaagggcgg atttcac
//
LOCUS       DAXHSF010000168         3819 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.168, whole genome shotgun sequence.
ACCESSION   DAXHSF010000168 DAXHSF010000000
VERSION     DAXHSF010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.168"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(<1..412)
                     /locus_tag="ACFM8S_001823"
     CDS             complement(<1..412)
                     /locus_tag="ACFM8S_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFC6314852.1"
                     /translation="MTHISSPRNEFIRGIKESSPMLIGLLPWALILGMQGGQKGMSWL
                     EMLLMTSMNFAGGSEFATVNLWAEPLPILLIATVTFMINSRHILMGAALAPHLKEIPL
                     KKAVPALFFMCDESWAMAFSEIQKRKAAGLPAFNM"
     gene            555..1007
                     /gene="dut"
                     /locus_tag="ACFM8S_001824"
     CDS             555..1007
                     /gene="dut"
                     /locus_tag="ACFM8S_001824"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFC6314853.1"
                     /translation="MNIEVEMKVLDERMADFVPVYATKGSAGLDLRACLDEEVVLQPG
                     ETFLVPTGLAIYLANPAYAAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGELKVSLWNR
                     SSEPFTVKPFERIAQMVIVPIVQARFKRVEEFVGSSRGECGFGSTGLH"
     gene            1085..2272
                     /gene="dapC"
                     /locus_tag="ACFM8S_001825"
     CDS             1085..2272
                     /gene="dapC"
                     /locus_tag="ACFM8S_001825"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HFC6314854.1"
                     /translation="MNTLLKQLKPYPFARLREAMQGISAPEGLEAVPLHIGEPKHPTP
                     KVITDALTASLHELEKYPLTAGLPELRQACANWLKRRYDGLTLNSDTEILPVLGSREA
                     LFSFVQTVLNPVSDGIKPAIVSPNPFYQIYEGATLLGGGEIHFANCPAPSFNPDWRSI
                     SEEVWKRTKLVFVCSPNNPSGSVLDLDGWKEVFDLQDKYGFIIASDECYSEIYFDGNK
                     PLGCLQAAAQLGRSRQKLLMFTSLSKRSNVPGLRSGFVAGDAELLKNFLLYRTYHGSA
                     MSIPVQRASIAAWNDEQHVIDNRRLYQEKFEWVIPILQQVFDVKLPDASFYIWLKVPD
                     GDDLAFARNLWQKAAIQVLPGRFLARDTEQGNPGEGYVRIALVADVATCVKAAETIVS
                     LYR"
     gene            2537..3316
                     /gene="yaaA"
                     /locus_tag="ACFM8S_001826"
     CDS             2537..3316
                     /gene="yaaA"
                     /locus_tag="ACFM8S_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFC6314855.1"
                     /translation="MFFVLSPAKNLNEKDPAPVSEFTQPDLLAESDILMQQLRELAPQ
                     QIAELMHVSDKIALLNAQRNAEWNTPFTPENAKQAVFMFNGDVYEGMDANTLDIGQIR
                     YLQNHVRLLSGLYGLLRPLDLIQPYRLEMGTAFANLRGKNLYEFWGDIITNLLNDTLA
                     QAGSNTLVNLASQEYFKSVNTKTLRARLITPIFKDEKNGKYKIISFYAKRARGLMVRY
                     AAEHHITDPEMLKNFNYEGYTFNDAASNESDWVFMRSEQIK"
     gene            3399..3489
                     /locus_tag="ACFM8S_001827"
     tRNA            3399..3489
                     /locus_tag="ACFM8S_001827"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3433..3435,aa:Ser,seq:tga)
     gene            3565..3654
                     /locus_tag="ACFM8S_001828"
     tRNA            3565..3654
                     /locus_tag="ACFM8S_001828"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3599..3601,aa:Ser,seq:cga)
BASE COUNT          978 a          896 c          919 g         1026 t
ORIGIN      
        1 gcatattgaa tgcgggcaaa ccggctgctt tccgtttttg gatttcggag aatgccatcg
       61 cccagctttc atcacacata aaaaacagtg cgggcacggc ttttttcagc ggtatttctt
      121 tcaggtgcgg ggcaagtgcc gcccccatca ggatatgccg cgaattaatc ataaaggtta
      181 cggtggcgat aagcagtatc ggcagaggct ccgcccacag gttgaccgtg gcaaattcgg
      241 agcctccggc aaaattcata ctggtcatca acaacatttc cagccagctc atgccttttt
      301 gtccgccctg cataccgagt attaatgccc aaggcaaaag cccgatcagc atgggcgaac
      361 tttctttgat gccgcgtata aattcgttac ggggcgagct tatgtgtgtc atattaatat
      421 tgatatgtaa gaaggtcggg cattatacac agacggagta tttcaaaaca aaaccgattt
      481 gccgtgtttc agcgtaaaca cggcttgtgt ataatctccc atctttgaaa tcggccgtat
      541 gcaggagcaa gacgatgaat attgaagtag aaatgaaagt attggacgaa cggatggcgg
      601 attttgtccc tgtctatgca acgaagggtt ctgcaggttt agatttgcgt gcctgtttgg
      661 atgaggaagt cgttttgcag ccgggtgaaa cgtttcttgt accgacaggt ttggcaattt
      721 atttggcgaa tcccgcatat gccgccgttt tgctgccccg ttccggattg gggcataaac
      781 acggcattgt tttgggcaat ttggtcggct tgattgactc cgattatcaa ggggaattga
      841 aggtgtcgtt atggaacaga agcagcgagc cgtttactgt caaaccgttt gagcgtatcg
      901 cgcagatggt tatcgtgcca atcgtgcagg cgcgtttcaa acgtgtcgaa gaatttgtcg
      961 gaagcagccg gggtgagtgc ggcttcggca gtacgggttt gcattgaata taaaatgccg
     1021 tctgatgggt gtcgtcaggt tcggacggca tatcttccca atatgccggt aatcggagaa
     1081 cattatgaat accttactca agcagcttaa accctatccc tttgcccgac tgcgtgaagc
     1141 gatgcagggc atttccgccc ccgaaggtct ggaagccgtc ccactgcata tcggcgaacc
     1201 gaaacatcca acaccgaaag tcattacaga tgcgctgacc gcctcattgc acgagttgga
     1261 aaaatatccg ctgacggccg gtttgcctga actgcgtcag gcgtgtgcga actggttaaa
     1321 acgccgttat gatgggttga cgctgaattc cgacaccgaa attctgccgg ttttgggcag
     1381 tagggaggcg ttgttttctt ttgttcaaac cgtgttgaac cctgtttcag acggcatcaa
     1441 acccgcaatt gtcagcccga atccctttta tcagatttac gaaggtgcga cacttttggg
     1501 cggcggtgaa atccattttg ccaattgccc cgcgccgtct ttcaaccccg attggcgtag
     1561 tatttccgaa gaggtttgga aacgcaccaa actggtgttc gtctgctcgc ccaacaaccc
     1621 cagcggcagc gtgctggatt tggacggatg gaaagaagtt tttgatttgc aggacaaata
     1681 tggtttcatt attgcctcgg acgaatgcta ttccgaaatc tatttcgacg gcaacaagcc
     1741 tttgggctgc ctgcaggccg ctgcacagtt ggggcgaagc aggcaaaaac tgcttatgtt
     1801 caccagtttg tccaagcggt ccaatgttcc gggcctgcgt tccggttttg tcgccggcga
     1861 tgccgaactg cttaaaaact ttctgcttta ccgaacttat cacggcagcg caatgagcat
     1921 tcccgtgcag cgtgccagta ttgccgcctg gaatgacgaa cagcacgtta ttgacaaccg
     1981 ccgcctgtat caggaaaagt ttgaatgggt tattcctatc ctgcaacagg tatttgacgt
     2041 caaattaccg gatgcctcgt tttacatctg gttgaaagtc cccgatggcg acgatttggc
     2101 atttgcacgc aatttatggc aaaaagccgc tatccaagta ttgcccggac gctttttggc
     2161 acgggatacc gaacagggca atcccgggga aggttatgtg cgtattgctt tggttgccga
     2221 tgtcgcaact tgtgtcaaag ctgcggaaac cattgtttcc ctatatcggt aaagaataaa
     2281 aaaatgccgt ccggactttc ctgacggcat tttttattat cgaaaccttt gcaatacctt
     2341 attttagata gttactgaaa ataaattact gagatttcaa ttaattattt ttgaattttg
     2401 aaatttggcg gttttgtaaa aatctctgtc attaaattgt gtttgccatt acggtatttg
     2461 catcacaatt aatcggcttg cctgaggatg cttttaaatc taaaattccg acgtttgaca
     2521 ccataccgag cagattatgt tttttgtcct ttcccctgcg aagaacctta atgaaaaaga
     2581 ccctgcccct gtcagcgagt ttacccaacc cgacctgctg gcagagtccg acattctaat
     2641 gcagcagttg cgcgagcttg cgccgcaaca gattgccgaa ctgatgcacg tttccgacaa
     2701 aattgccctc ttaaacgcgc agcgcaatgc agaatggaac acgccgttta cgccggaaaa
     2761 cgccaaacag gcggtcttta tgttcaacgg cgatgtttac gaaggtatgg atgcaaacac
     2821 attggatatt ggacagatac gctatctgca aaaccatgtc cgcctgctgt ccggtctgta
     2881 cggtcttctt cgcccgttag acctgataca gccctatcgt ttggaaatgg ggacggcatt
     2941 tgccaatttg cgcggcaaga atttgtatga gttttggggc gacatcatta ccaacctttt
     3001 aaatgacacg cttgcccaag caggcagcaa tacgcttgtc aaccttgcct cacaggaata
     3061 tttcaagtcc gtcaacacga aaacacttcg ggcgcggctg attaccccga tatttaaaga
     3121 cgaaaaaaac ggtaaatata aaatcatcag tttctatgcc aagcgtgcgc gtggattaat
     3181 ggtgcgctat gcggcagaac atcatattac cgaccctgaa atgctgaaaa attttaatta
     3241 cgaaggttac acattcaatg atgcggcctc aaatgaaagc gattgggtgt ttatgcgttc
     3301 ggaacaaata aagtgaaaac aataaattaa gtattttccg aaaaaagtgc ttggcaaaat
     3361 gtataaattt cattattatc cctaatcttc aagaagacgg aagcgtggca gagcggttta
     3421 atgcaacggt cttgaaaacc gtcgagggtt gatagccctc cgtgagttcg aatctcaccg
     3481 cttccgccat ttcttgagag tagaaccaaa taagaatgca atagtagcaa atattgtatt
     3541 tttatttggt tttactgcat tatcggaaac gtggcagaga ggctgaatgc agcggactcg
     3601 aaatccgctg agggtgcaaa tcctccgtgg gttcgaatcc caccgtttcc gccacaaaac
     3661 aaaaccgccc tgattcgggg cggttctttt ttgttcaagt tgagaccttt gcaaaattcc
     3721 tttccttccc gacagccgaa acccaaacac aggttttcgg ctgttttcgc cccaaatacc
     3781 gcctaatttt acccaaatat ccccttaatc ctgcccgga
//
LOCUS       DAXHSF010000169         3795 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.169, whole genome shotgun sequence.
ACCESSION   DAXHSF010000169 DAXHSF010000000
VERSION     DAXHSF010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.169"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..210
                     /locus_tag="ACFM8S_001829"
     CDS             <1..210
                     /locus_tag="ACFM8S_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HFC6314856.1"
                     /translation="HGFCVLGDAAEVVYKCTDYYKPEAEQVLIWNDPTVGIGWPLQTA
                     PLLSPKDLAGKAWAQAEKLRLTLSR"
     gene            complement(254..2272)
                     /locus_tag="ACFM8S_001830"
     CDS             complement(254..2272)
                     /locus_tag="ACFM8S_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HFC6314857.1"
                     /translation="MNKSLSGSVEEYRELTLRGMILGALITVIFTASNVYLGLKVGLT
                     FASSIPAAVISMAVLKFFKGSNILENNMVQTQASAAGTLSTIIFVLPGLLMAGYWSGF
                     PFWQTTLLCIAGGILGVIFTIPLRYAMVVKSDLPYPEGVAAAEILKVGGHEEGDNRQG
                     GSGIKELAAGGALAGLMSFCAGGLRVIADSASYWFKSGTAIFQLPMGFSLALLGAGYL
                     VGLTGGIAILLGISIAWGIAVPYFSSHIPQPSDMEMAAFAMKLWKEKVRFIGAGTIGI
                     AAVWTLLMLLKPMVEGMKMSFKSFGGGAPAAERAEQDLSPKAMIFWVLAMMFVLGVSF
                     YHFIGDSHITGGMAWLLVVVCTLLASVIGFLVAAACGYMAGLVGSSSSPISGVGIVSV
                     VVISLVLLLVGESGGLLADEANRKFLLALTLFCGSAVICVASISNDNLQDLKTGYLLK
                     ATPWRQQVALIIGCIVGALVISPVLELLYEAYGFTGAMPREGMDAAQALAAPQATLMT
                     TIASGIFAHNLEWVYIFTGIAIGAVLIVVDLVLKKSSGGKLALPVLAVGMGIYLPPSV
                     NMPIVAGAVLAAVLKHIIGKKAENREGRLKNAERIGTLFSAGLIVGESLIGVIMAFII
                     AFSVTNGGSDAPLALNLQNWDAAASWLGLAFFVTGMFFFAQRVLKAGR"
     gene            complement(2468..3589)
                     /gene="dnaJ"
                     /locus_tag="ACFM8S_001831"
     CDS             complement(2468..3589)
                     /gene="dnaJ"
                     /locus_tag="ACFM8S_001831"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFC6314858.1"
                     /translation="MSNQDFYATLGVARTATDDEIKKAYRKLAMKYHPDRNPDNKEAE
                     EKFKEVQKAYETLSDKEKRAMYDQYGHAAFEGGGQGGFGGFGGFGGAQGFDFGDIFSQ
                     MFGGGSGRAQPDYQGEDVQVGIEITLEEAAKGVKKRINIPTYEACDVCNGSGAKPGTS
                     PETCPTCKGSGTVHIQQAIFRMQQTCPTCHGAGKHIKEPCVKCRGAGRNKAVKTVEVN
                     IPAGIDDGQRIRLSGEGGPGMHGAPAGDLYVTVRIRAHKIFQRDGLDLHCELPISFAT
                     AALGGELEVPTLDGKVKLTVPKETQTGRRMRVKGKGVKSLRSSATGDLYCHIVVETPV
                     NLTDRQKELLEEFERISTGLENQTPRKKSFLDKLRDLFD"
BASE COUNT         1005 a         1132 c          859 g          799 t
ORIGIN      
        1 cacgggtttt gcgttttggg cgacgcagcg gaagtcgtgt acaaatgcac ggactattac
       61 aagcctgaag ccgaacaggt tttaatatgg aacgacccga cagtcggcat aggctggccg
      121 cttcaaaccg cgccgctgct gtcgcccaaa gatcttgccg gtaaagcgtg ggcgcaagcc
      181 gaaaagctcc gccttacgct ttcccgataa aaaatgccgt ctgaacgttt cagacggcat
      241 tttttccgac aggctaccta cccgccttca gtacgcgctg tgcaaagaaa aacatcccgg
      301 taacgaagaa cgccaagccc aaccatgagg cggcggcatc ccagttttgc agattcaacg
      361 caagcggcgc atccgagccg ccgttggtca cggagaaggc aataataaac gccataatca
      421 caccgatcag gctttcaccg acaatcaggc cggcggagaa caaggttccg atgcgctcgg
      481 cgtttttcag acggccttca cggttttccg cttttttacc gatgatgtgt ttcaacactg
      541 ccgccaacac cgcgcctgcc acgatgggca tattgacgga cggcggcaga taaataccca
      601 taccgaccgc aaggacgggc agggcaagtt taccgcctga tgattttttc aacaccaaat
      661 cgacgacgat taataccgct ccaatcgcga tgccggtaaa gatgtagacc cattcgaggt
      721 tgtgggcgaa aatgcccgac gcgatggtcg tcatcaaagt cgcttgaggt gctgccaaag
      781 cctgcgccgc atccatgcct tcgcgcggca ttgcgccggt aaagccgtag gcttcgtaaa
      841 gcagttccaa cacgggcgaa ataaccagcg caccaacgat acagccgata atcagggcga
      901 cttgctgccg ccaaggcgtg gctttgagca ggtagccggt tttcaagtct tgcaggttgt
      961 cattggaaat cgaagccacg cagattactg ccgagccgca aaacaaagtc agtgccagca
     1021 aaaatttgcg gttagcctca tccgccaaca aacctccgga ttcgcctacc agcagcaaaa
     1081 ccagtgaaat aacgacgacg gacacgatgc ccacgccgga aatcgggctg gaagacgagc
     1141 cgaccaaacc tgccatataa ccgcaggcgg cggcgaccaa aaagccgatg acggaagcca
     1201 aaagcgtgca aacgaccacc aaaagccaag ccatgccgcc cgtaatgtgc gaatcgccga
     1261 taaagtggta aaacgacacg cctaaaacaa acatcatcgc cagcacccaa aaaatcatag
     1321 ccttaggcga caaatcctgt tcggcgcgtt ccgcagcggg cgcaccgccg ccaaaactct
     1381 tgaacgacat cttcatgcct tccaccatcg gcttgagcag catcaacagc gtccaaaccg
     1441 ccgcaatgcc aatagtcccc gcaccgataa aacgcacttt ctccttccac agcttcatcg
     1501 caaacgccgc catttccata tcggaaggtt gcggaatgtg tgaggagaaa tacggcacgg
     1561 caatgcccca agcaatcgaa atgcccaaca ggatggcgat accacccgtc agtccgacca
     1621 aatagcccgc gcccaacaat gccagtgaaa agcccatcgg cagctggaaa atcgccgtac
     1681 cgcttttaaa ccaataactc gcgctgtcgg caatcacgcg cagacctccg gcgcaaaagc
     1741 tcatcaatcc cgccaacgca ccgccggccg ccagctcttt gatgccgctg ccgccctgac
     1801 ggttatcccc ttcttcatga ccgcccactt tcaaaatttc agcagccgcc acaccttccg
     1861 gataaggcaa atcgcttttc accaccatcg cgtaacgcag cggaatggtg aaaatcaccc
     1921 ccaaaatccc gccggcaata cataaaagcg tcgtctgcca gaacgggaaa ccgctccagt
     1981 agcccgccat cagcaaaccg ggcaggacga agatgatggt cgaaagcgta cccgccgccg
     2041 aagcttgggt ctgcaccatg ttgttttcca aaatattgct gcctttgaaa aactttaaaa
     2101 ccgccatcga aatcaccgcc gccggaatcg acgaggcaaa ggtcagcccg actttcaaac
     2161 cgaggtaaac gttggacgca gtaaaaatta cagtgatcaa tgcaccgagt atcatgcctc
     2221 ggagcgtcag ttcgcggtat tcttctaccg acccggataa agatttattc attattcttc
     2281 ctttgacaac agacgttcac atattgttgg catcgcgcca tgatgtcaag ttttaaaaag
     2341 aacagttaaa aacagttatc ccaccctgcc ccatacccca ttgaaaataa aaactatttt
     2401 taaacaaaca aaaacagccg tatcagggag attccccgat acggctgctt gtttccgaac
     2461 cttaaaatca atcaaacaaa tcgcgcagct tgtctaaaaa cgatttcttg cgcggtgttt
     2521 ggttttccaa gccggtagaa atccgctcaa attcttccaa aagctctttt tgacggtcgg
     2581 tcaaattgac aggcgtttcg acaacaatat ggcagtacaa atcgccggtc gcgctgctgc
     2641 gtaaagattt gacaccctta cccttcacgc gcatcctcct gccggtttgg gtttctttgg
     2701 ggacggtgag cttgaccttt ccgtccaagg tcggcacttc caactccccg cccaaagcag
     2761 ccgtggcaaa actgatcggc agttcgcaat gcaagtccag accgtcgcgt tggaaaatct
     2821 tatgcgcccg aatgcggacg gttacataca ggtcgccggc aggcgcaccg tgcatacccg
     2881 gtccgccttc gccgctcaaa cggatacgct gcccgtcatc gataccggcg ggaatattga
     2941 cttccaccgt cttgaccgcc ttattccgcc ccgcgccacg gcatttgacg caaggttctt
     3001 taatgtgttt gcccgcaccg tggcaggtcg gacaagtctg ctgcatacgg aaaatcgcct
     3061 gctggatgtg caccgtaccc gaacctttgc aagtcgggca ggtttccggg gatgtccccg
     3121 gtttcgcgcc actgccgtta cagacatcac acgcttcata agtcggaata ttgatgcgtt
     3181 tcttcacacc ttttgcggct tcttcaagcg tgatttcgat accgacttga acgtcctcac
     3241 cctgataatc aggctgggcg cgccccgaac cgcctccaaa catttggctg aaaatatccc
     3301 caaagtcaaa accctgcgca ccgccaaatc cgccaaaccc tccgaagccc ccctgtccgc
     3361 cgccttcaaa cgccgcatga ccatactggt cgtacatagc gcgcttttcc ttgtcggaca
     3421 aagtttcata cgccttttgt acttctttaa acttctcttc cgcctcttta ttgtcaggat
     3481 tgcggtcggg atggtatttc attgccaatt tccggtaggc ttttttaatc tcatcatcgg
     3541 tagctgttct tgccacaccc agcgtcgcat aaaaatcttg attactcatt ttttcatcta
     3601 attcaaaata aaatcacggc tcaaaataag ggcaattgcg caaaatacaa gacaaacaga
     3661 ctgccatagc ttacaaactg aaacggaata cacttttcag acagcataaa ccgatgccgt
     3721 ctgaaatctt caggtatgca cgacacaaaa acttagacag gcataaaacc aaccgccatg
     3781 aagtgttttt gttat
//
LOCUS       DAXHSF010000170         3775 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.170, whole genome shotgun sequence.
ACCESSION   DAXHSF010000170 DAXHSF010000000
VERSION     DAXHSF010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.170"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            74..703
                     /locus_tag="ACFM8S_001832"
     CDS             74..703
                     /locus_tag="ACFM8S_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HFC6314859.1"
                     /translation="MSETENQALTFAKRLKADTTAVHDSVDNLVMSVQPFVSKENYIK
                     FLKLQSVFHKAVDHIYKDAELNKAIPELEYMARYDAVTQDLADLGEEPYKFDKELPHE
                     TGNKAIGWLYCAEGSNLGAAFLFKHAQKLDYNGEHGARHLAPHPDGRGKHWRAFVEHL
                     NALNLTPEAEAEAIQGAREAFAFYKVVLRETFGLAADAEAPEGMMPHRH"
     gene            878..3259
                     /gene="hmbR"
                     /locus_tag="ACFM8S_001833"
     CDS             878..3259
                     /gene="hmbR"
                     /locus_tag="ACFM8S_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent hemoglobin receptor HmbR"
                     /protein_id="HFC6314860.1"
                     /translation="MKPLQMLPIAALVGSIFGNPVFAADEAATETTPVKAEVKAVRVK
                     GQRNAPAAVERVNLNRIKQEMIRDNKDLVRYSTDVGLSDSGRHQKGFAVRGVEGNRVG
                     VSIDGVNLPDSEENSLYARYGNFNSSRLSIDPELVRNIDIVKGADSFNTGSGALGGGV
                     NYQTLQGRDLLLPERQFGVMMKNGYSTRNREWTNTLGFGVSNDRVDAALLYSQRRGHE
                     TESAGKRGYPVEGAGSGANIRGSARGIPDPSQHKYHSFLGKIAYQINDNHRIGASLNG
                     QQGHNYTVEESYNLLASYWREADDVNRRRNTNLFYEWTPESDRLSMVKADVDYQKTKV
                     SAVNYKGSFPIEDSSTLTRNYNQKDLDEIYNRSMDTRFKRITLRLDSHPLQLGGGGRH
                     RLSFKTFASRRDFENLNRDDYYFSGRVVRTTSSIQHPVKTTNYGFSLSDQIQWNDVFS
                     SRAGIRYDHTKMTPQELNAECHACDKTPPAANTYKGWSGFVGLAAQLNQAWRVGYDIT
                     SGYRVPNASEVYFTYNHGSGNWLPNPNLKAERSTTHTLSLQGRSEKGTLDANLYQSNY
                     RNFLSEEQKLTTSGDVSCTQMNYYYGMCSNPYSEKLEWQMQNIDKARIRGIELTGRLN
                     VDKVASFVPEGWKLFGSLGYAKSKLSGDNSLLSTQPLKVIAGIDYESPSEKWGVFSRL
                     TYLGAKKVKDAQYTVYENKGWGTPLQKKVKDYPWLNKSAYVFDMYGFYKPAKNLTLRA
                     GVYNVFNRKYTTWDSLRGLYSYSTTNAVDRDGKGLDRYRAPGRNYAVSLEWKF"
     gene            complement(3466..3588)
                     /locus_tag="ACFM8S_001834"
                     /pseudo
     CDS             complement(3466..3588)
                     /locus_tag="ACFM8S_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
BASE COUNT         1017 a          964 c          948 g          846 t
ORIGIN      
        1 tccactatat atgcgggcaa gtgcggattt tgcccgcccc gcatccttat cccttaccga
       61 caaaggaaaa cttatgagtg aaaccgaaaa tcaagccctg acgtttgcca aacgtctgaa
      121 ggcggatacc acggcggttc acgacagcgt ggataacctc gttatgtctg tccaaccgtt
      181 tgtcagcaaa gaaaactaca tcaaattttt gaaactccaa tccgttttcc acaaggccgt
      241 cgaccacatc tataaagatg ccgaattaaa caaagccatt cccgagctgg aatacatggc
      301 gcgatacgat gccgtaacgc aagaccttgc agatttgggc gaagagcctt acaaattcga
      361 caaagaatta ccgcatgaaa ccggcaacaa agcaatcggc tggctttatt gcgccgaagg
      421 atccaatttg ggcgcggcgt ttttgttcaa acacgcccaa aaactcgatt acaacggcga
      481 acacggcgcg cgccacctcg ctccgcatcc cgacgggcgc ggcaaacact ggcgcgcctt
      541 cgtcgagcat ctgaacgctt tgaacctgac tcccgaagcc gaagcggaag ccattcaagg
      601 cgcgcgcgaa gcctttgcat tctacaaagt cgtgttgcgc gaaaccttcg gcttggcagc
      661 cgatgccgaa gcccccgaag gtatgatgcc gcacaggcac taaaaaataa tcgaaccaaa
      721 taaacaaggt ctcggcatag ctgtttgcag ggacctttaa ttacacggcg cggctttgtt
      781 tacatggatt actgtcttat taaatattaa tgattatcat aaaatctatt attcgctaac
      841 cgatggatga acaatccata catcttgagt tgataatatg aaaccattac aaatgctccc
      901 tatcgccgcg ctggtcggca gtattttcgg caatccggtc tttgcggcag atgaagctgc
      961 aactgaaacc acacccgtta aggcagaggt aaaagcagtg cgcgttaaag gccagcgcaa
     1021 tgcgcctgcg gctgtggaac gcgtcaacct taaccgtatc aaacaagaaa tgatacgcga
     1081 caacaaagac ttggtgcgct attccaccga tgtcggcttg agcgacagcg gccgccatca
     1141 aaaaggcttt gctgttcgcg gcgtggaagg caaccgtgtc ggcgtgagca tagacggcgt
     1201 aaacctgcct gattccgaag aaaactcgct gtacgcccgt tatggcaact tcaacagctc
     1261 gcgtctgtct atcgaccccg aactcgtgcg caacatcgac atcgtaaaag gggcggactc
     1321 tttcaatacc ggcagcggcg ccttgggcgg cggtgtgaat taccaaaccc tgcaaggacg
     1381 tgacttactg ttgcctgaac ggcagttcgg cgtgatgatg aaaaacggtt acagcacgcg
     1441 taaccgtgaa tggacaaata ccctcggttt cggcgtgagc aacgaccgcg tggatgccgc
     1501 tttgctgtat tcgcaacggc gcggccatga aactgaaagc gcgggcaagc gtggttatcc
     1561 ggtagagggt gctggtagcg gagcgaatat ccgtggttct gcgcgcggta ttcctgatcc
     1621 gtcccaacac aaataccaca gcttcttggg taagattgct tatcaaatca acgacaacca
     1681 ccgcatcggc gcatcgctca acggtcagca ggggcataat tacacggttg aagagtctta
     1741 caacctgctt gcttcttatt ggcgtgaagc tgacgatgtc aacagacggc gtaacaccaa
     1801 cctcttttac gaatggacgc cggaatccga ccggttgtct atggtaaaag cggatgtcga
     1861 ttatcaaaaa accaaagtat ctgcggtcaa ctacaaaggt tcgttcccga tagaggattc
     1921 ttccaccttg acacgtaact acaatcaaaa ggacttggat gaaatctaca accgcagtat
     1981 ggatacccgc ttcaaacgca ttaccctgcg tttggacagc catccgttgc aactcggggg
     2041 gggggggcga caccgcctgt cgtttaaaac tttcgccagc cgccgtgatt ttgaaaacct
     2101 aaaccgcgac gattattact tcagcggccg tgttgttcga accaccagca gtatccagca
     2161 tccggtgaaa accaccaact acggtttctc actgtctgac caaattcaat ggaacgacgt
     2221 gttcagtagc cgcgcaggta tccgttacga tcataccaaa atgacgcctc aggaattgaa
     2281 tgccgagtgt catgcttgtg acaaaacacc gcctgcagcc aacacttata aaggctggag
     2341 cggttttgtc ggcttggcgg cgcaactgaa tcaggcttgg cgtgtcggtt acgacattac
     2401 ttccggctac cgtgtcccca atgcgtccga agtgtatttc acttacaacc acggttcggg
     2461 taattggctg cccaatccca acctgaaagc cgagcgcagc accacccaca ccctctctct
     2521 gcaaggccgc agcgaaaaag gtactttgga tgccaacctg tatcaaagca attaccgcaa
     2581 tttcctgtct gaagagcaga agctgaccac cagcggcgat gtcagctgta ctcagatgaa
     2641 ttactactac ggtatgtgta gcaatcctta ttccgaaaaa ctggaatggc agatgcaaaa
     2701 tatcgacaag gccagaatcc gcggtatcga gctgacaggc cgtctgaatg tggacaaagt
     2761 agcgtctttt gttcctgagg gctggaaact gttcggctcg ctgggttatg cgaaaagcaa
     2821 actgtcgggt gacaacagcc tgctgtccac acagccgctg aaagtgattg ccggtatcga
     2881 ctatgaaagt ccgagcgaaa aatggggcgt gttctcccgc ctgacctatc tgggcgcgaa
     2941 aaaggtcaaa gacgcgcaat acaccgttta tgaaaacaag ggctggggta cgcctttgca
     3001 gaaaaaggta aaagattacc cgtggctgaa caagtcggct tatgtgtttg atatgtacgg
     3061 cttctacaaa ccggctaaaa acctgacttt gcgtgcaggc gtatataatg tgttcaaccg
     3121 caaatacacc acttgggatt ccctgcgcgg tttgtatagc tacagcacca ccaatgcggt
     3181 cgaccgcgat ggcaaaggct tagaccgcta ccgcgcccca ggccgcaatt acgccgtatc
     3241 gctggaatgg aagttttaat ctggtattat tgaattaagt cttgttgaaa attaaagccg
     3301 tccgaattgt gttcaagaac tcattcggac ggctttttac cgtaaatctg tgtgatgggg
     3361 ttttatattg atacagtatc cgaactcagg gcaggaagta atgttgcagg attaaaaatg
     3421 ccgtctgaaa aaatgtttca gacggcattt ttggttgccg atatgttaac ggacgacatt
     3481 ggcgcgcatg accacgcgga cgtggaacga gcctgcctga cagtcgtatt gtccgccttg
     3541 cagcggctgc ttgtcgaagt agctgtggaa gccggtaacg ccgcgtccgc aagggtggcg
     3601 gcagatgccg cattgtgttt ataatgccgt gtcagacgtt tgatttatag gaaaataatg
     3661 atgcttacca atcaggaagt aaccggtgtc aaactgggat accgcaaagc cgttttatag
     3721 tggattaaca aaaatcagga caaggcgacg aagccgcaga cagtacaaac agtac
//
LOCUS       DAXHSF010000171         3739 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.171, whole genome shotgun sequence.
ACCESSION   DAXHSF010000171 DAXHSF010000000
VERSION     DAXHSF010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.171"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(80..864)
                     /locus_tag="ACFM8S_001835"
                     /pseudo
     CDS             complement(80..864)
                     /locus_tag="ACFM8S_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259846.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
     gene            complement(944..2194)
                     /locus_tag="ACFM8S_001836"
     CDS             complement(944..2194)
                     /locus_tag="ACFM8S_001836"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFC6314861.1"
                     /translation="MTKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAVRIWPS
                     HTYTRLTLESTTALKYQHFTLDNPSRLVVDIQNANINTVLHGLSQKVMADDPFIRSIR
                     AGQNTPTTVRLVIDLKQPSYAQVFSLPPVGGFKNRLVVDLYPQGMDPDDPMMALLNGS
                     LNKTRRGSPEADLAQNTPAQTERGKNGRRPVIMIDPGHGGEDPGAVSPGGLQEKHVVL
                     SIARETKKQLEALGYNVFMTRNEDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSAR
                     GTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTALLDMTQTATLRDSR
                     KLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESF
                     RRQCAQSIASGVQRYINTSVLKQG"
     gene            complement(2191..2652)
                     /gene="tsaE"
                     /locus_tag="ACFM8S_001837"
     CDS             complement(2191..2652)
                     /gene="tsaE"
                     /locus_tag="ACFM8S_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFC6314862.1"
                     /translation="MSDLPSISRFLADEAATLDLGAAWSSRLNAPLVIYLEGDLGAGK
                     TTLTRGILRGLGHLGAVKSPTYAIVESYPLKPFTLHHFDLYRFSFPEEWEDAGLDELF
                     AANSVCLIEWPQQGGEFTPPADITATLTHDGDGRKCLLTAHTERGRESLPL"
     gene            2854..3666
                     /gene="murI"
                     /locus_tag="ACFM8S_001838"
     CDS             2854..3666
                     /gene="murI"
                     /locus_tag="ACFM8S_001838"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFC6314863.1"
                     /translation="MENIGRQRPIGVFDSGIGGLTNVRALMERLPMENIIYFGDTARV
                     PYGTKSKATIENFSMQIVDFLLEHDVKAMVIACNTIAAVAGQKIRQKTGNMPVLDVIS
                     AGAKAALATTRNNKIGIIATNTTVNSNAYARAIHRDNPDTLVRTQAAPLLVPLVEEGW
                     LEHEVTRLTVCEYLKPLLADGIDTLVLGCTHFPLLKPLIGREAHNVALVDSAITTAEE
                     TARVLAQEGLLNTDNNNPDYRFYVSDIPLKFRTIGERFLGRTMEQIEMVSLG"
BASE COUNT          835 a          905 c         1111 g          888 t
ORIGIN      
        1 ccgaaggtaa gggaggggcg tgtccatttt gccgccgcat ttgccgcgtg cggaagatac
       61 ggataacaac cccttggaat taaagggaga agttgcggaa aatatccaca tcaggctggg
      121 gaccgtactt atttacggta ttttcgtatg ccgaaataaa atatttcttg aataaaccgt
      181 aatcgccggt ttcgtaatag gctacgacag cctcggcata ggcgggataa ctgtcggggg
      241 cgaatacgca aggaaaaaaa ccggagcgca tcagcgacaa ggtcatacag tttctggcag
      301 tacgtttatt gcaatcttta aaatattgaa gatacgccaa attattatgg agataaacgg
      361 cgcggtcgaa gggatttcct atttttgggg cttcttgaag caaccatttt aattccgtat
      421 ccaaactttt atagtaacgc tgtcgcggcg gactacgccg ccggagcctt tttccagtaa
      481 gttttccgaa atcaggctgt gggtggtttt taagaaatcc aaccagtcaa acggatcggg
      541 gcggtccaaa ccggacagca ggtgccggta actttccctc aggttgatta acattacggc
      601 atccgaatat aacttcccgc ctgcggtttg cccgagttta agcaatgcct gcgtatcgta
      661 ttgattataa gtgtttcctt ccaatttggc agaactgtac acaaaatcga taccgatttc
      721 ttcaatatta cgcacggagt ccgttaaaac agataaagga agccgatcgg acaattcggt
      781 caggtattct tgttcgccgg ctgtaaaaat ggggcggata tttaaaaaat cctgttggta
      841 tctcaattta tccatacctt tcatcataaa aacgcggttt ggggcggaag taggtttatg
      901 cctgccttca tccgacaact atattgaaac accgcctttt caatcaaccc tgcttcaata
      961 cggaagtgtt gatgtagcgt tggacacccg aggcaatgga ttgggcgcac tgtcggcgga
     1021 aggattcgct gcccagcagc ttctcttcgg cagggttgga caggaaggcg gtttcgacca
     1081 ggatagacgg catatcgggt gcgcgcaaaa cggcgaaatt ggcttcgtcc accctgcctt
     1141 tgtgcagatg gttgagcctg cccaattctt caagcaccag tttgccgagt ttgcggctgt
     1201 cgcgcagcgt ggcggtttgg gtcatgtcga gcagggcggt atcgacattg cggttgccgc
     1261 tggtcggtac gccgccgacc gcgtcggcat tgttttgcgt ctgttccaag aatttggcgg
     1321 cggagctggt cgcgcctttg gtgttcagca tataaacccc cgttccgcgc gcggaggggc
     1381 tggtgaaggc atcggcgtgg atggagacaa atacgtccgc ccgccgtgcg cgccctttgg
     1441 cgacacgcac gcccaaaggg atgaacacgt cttcgttgcg cgtcataaag acgttgtaac
     1501 ccaaggcttc caattgtttt ttggtttcgc gggcgatgga gaggacgacg tgtttttcct
     1561 gcaaaccgcc cgggctgacg gcgccggggt cttcgccgcc gtgtccgggg tcgatcataa
     1621 tgacgggtct gcgcccgttt ttgccgcgtt cggtttgtgc gggagtgttt tgagcgaggt
     1681 cggcttcagg ggagccgcga cgggttttgt tcaggctgcc gttaagcagc gccatcatcg
     1741 gatcgtcggg atccatccct tgcggataga gatcgacgac gaggcggttt ttaaagccgc
     1801 cgacgggggg gagggaaaaa acttgcgcgt aggaaggctg tttcaaatcg atgacgaggc
     1861 ggacggtggt cggcgtgttc tgacccgcgc gtatgctgcg gataaagggg tcgtccgcca
     1921 taaccttctg agacagtccg tgcaatacgg tattgatgtt cgcgttttgt atgtcgacga
     1981 ccagcctgct tgggttgtcg agcgtgaagt gttggtattt gagcgcggtt gtgctttcca
     2041 gcgtcaggcg ggtgtaggtg tgcgacggcc atatccgtac ggcggtaaac tgcggggcgc
     2101 gtaccgtttt ggcaacggcg gatgcgatgg ggcttagggc gaacagtgtg ccggcggtgc
     2161 ggcggatgat ttgtcttcgt gtcagtttgg tcatagcggc aggctttcgc gtcctcgttc
     2221 ggtatgggcg gtcagcaggc attttctgcc gtcgccgtcg tgtgtcaatg ttgcggtgat
     2281 gtcggcaggc ggcgtaaatt ccccgccctg ttgcggccat tcgatcaggc agacgctgtt
     2341 tgcggcaaac agttcgtcaa gccccgcgtc ttcccattct tcggggaacg agaagcggta
     2401 gaggtcgaaa tggtgcaggg tgaagggttt cagcggatag gattcgacga tggcgtaagt
     2461 cgggcttttg actgcgccca gatgacctaa tccgcgcagg atgccgcgtg tcagcgtggt
     2521 tttacccgca cccaaatccc cctcgagata aatgaccagc ggcgcgttta aacgggaaga
     2581 ccacgccgcg cccaaatcga gtgtggcggc ttcgtcggca aggaatcggg agatagaggg
     2641 taaatcagac atggaaacgg tttgttgtaa ggtctagggt attatgggca gttttgcagg
     2701 ttttgcaaac tttgcacccg aggggcggat gcttcttgtc cgagcattat aacagccaaa
     2761 tccgcgttct gctttcagac ggcaacggct gtcaagaaaa agcggcgcgt gtacaatacg
     2821 cggattgtat gtttaggacg gattggaaaa aggatggaaa atatcggcag gcagcgaccc
     2881 atcggcgttt ttgactcggg aatcggcggt ttgaccaatg tacgcgcgct gatggagcgg
     2941 ctgccgatgg agaacatcat ttatttcggc gacacggcgc gcgtgcctta cgggacgaaa
     3001 tctaaggcta ccatcgaaaa tttctcgatg cagattgtcg attttttatt ggaacacgat
     3061 gttaaggcga tggttatcgc gtgcaatacg attgcggcgg tggcggggca gaaaatccgt
     3121 caaaagacgg gcaatatgcc tgtgttggac gtgatttccg ccggcgcgaa agccgcgctg
     3181 gcaacgacgc gcaacaataa aatcggcatt atcgccacca ataccacagt taacagcaac
     3241 gcctatgcgc gcgccatcca cagggacaac cccgacacgc tcgtccgcac gcaggccgcg
     3301 ccgctgctcg tccctttggt ggaagagggc tggttggaac acgaagttac ccgcctgacc
     3361 gtatgcgaat acctcaaacc attgcttgca gacggcatcg atacgctggt gttgggctgc
     3421 acgcactttc ccttgctcaa gcccttaatc ggcagggagg cgcacaatgt cgcgctggtc
     3481 gattccgcga ttacgactgc cgaagaaacc gcacgcgtcc ttgcgcagga gggattactc
     3541 aataccgaca acaacaatcc cgactatcgt ttttatgtca gcgatattcc tttgaaattc
     3601 agaacaatag gcgagcgttt tctgggcagg acgatggagc agattgaaat ggtgtctttg
     3661 ggttaaaacg atgacggaaa gctgcccgag attacagaaa cctaaaatcc cgtcattccc
     3721 acgaaagtgg gaatctaga
//
LOCUS       DAXHSF010000172         3649 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.172, whole genome shotgun sequence.
ACCESSION   DAXHSF010000172 DAXHSF010000000
VERSION     DAXHSF010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.172"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            275..3478
                     /gene="nalP"
                     /locus_tag="ACFM8S_001839"
     CDS             275..3478
                     /gene="nalP"
                     /locus_tag="ACFM8S_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endopeptidase autotransporter NalP"
                     /protein_id="HFC6314864.1"
                     /translation="MRTTPTFPTKTFKPAAMALAVATTLSACLGGGGGTSAPDFNAGG
                     TGIGSNSRATTAKSAAVSYAGIKNEMCKDRSMLCAGRDDVAVTDRDAKINAPPPNLHT
                     GDFTNPNDAYKNLINLKPAIEAGYTGRGVEVGIVDTGESVGSISFPELYGRKEHGYNE
                     NYKNYTAYMRKEAPEDGGGKDIEASFDDEAVIETEAKPTDIRHVKEIGHIDVVSHIIG
                     GRSVDGRPAGGIAPDATLHIMNTHDGTKNEIMSAAIRNAWVKLGERGVRIVNNSFGTT
                     SRAGTADHFQIANSEEQYRQALLAYSGGDKTDEGIRLMQQSNYGNLSYHIRNKNMLFI
                     FSASNDAQAQPNTLTLLPFYEKDAQKGIITVAGVDRSGEKFNGSNHCGITAMWCLSAP
                     YEASVRFTRTNPIQIAGTSFSAPIVTGTAALLLQKYPWMSNDNLRTTLLTTAQDIGAV
                     GVDSKFGWGLLDAGKAMNGPASFPFGDFTADTKGTSDIAYSFRNDISGTGGLIKKGGS
                     QLQLHGNNTYTGKTIIEGGSLVLYGNNKSDMRVETKGALIYNGAASGGSLNSDGIVYL
                     ADTDRSGANETVHIKGDLQLGGEGTLYTRLGKLLKVDGTAMTGGKLYMSARGKGAGYL
                     NRTGQRVPFLSAAKIGRDYSFFTNIETDGGLLASLDSVEKTAGSEGDTLSYYVRRGNA
                     ARTASAAAHSAPAGLKHAVEQGGSNLENLMVELDASESSATPETVETAAADRTDMPGI
                     RPYGATFRAAAAVQHANAADGVRIFNSLAATVYADSTAAHADMQGRRLKAVSDGLDHN
                     GTGLRVIAQTQQDGGTWEQGGVEGKMRGSTQTVGIAAKTGENTTAAATLGMGRSTWSE
                     NSANAKTDSISLFAGIRHDAGDIGYLKGLFSYGRYKNSISRSTGADEHAEGSVNGTLM
                     QLGALGGVNVPFAATGDLTVEGGLRYDLLKQDAFAEKGSALGWSGNSLTEGTLVGLAG
                     LKLSQPLSDKAVLFATAGVERDLNGRDYTVTGGFTGATAATGKTGARNMPHTRLVAGL
                     GADVEFGNGWNGLARYSYAGSKQYGNHSGRVGVGYRF"
BASE COUNT          988 a         1000 c          974 g          687 t
ORIGIN      
        1 atccactata actagggaaa ttcaaattaa gttagaatta tccctatgag aaaaagccgt
       61 ctaagccggt ataaacaaaa taaactcatt gagctatttg tcgaaagttc aaatttccat
      121 tttaaaacaa ttagtaaaat cgagtttatc ctagttgtcc aagacaaccc ctataataat
      181 ataattcaaa atataaaaat gggttacatc taaacattac ggaattttta ttccctcgcc
      241 tgaattctat tgtcagattc aaggagacct catcatgcga acgaccccaa ccttccctac
      301 aaaaactttc aaaccggctg ccatggcgtt agctgttgca acaacacttt ctgcctgctt
      361 aggcggcggc ggcggcactt ctgcgcccga cttcaatgca ggcggcaccg gtatcggcag
      421 caacagcaga gcaacaacag cgaaatcagc agcagtatct tacgccggta tcaagaacga
      481 aatgtgcaaa gacagaagca tgctctgtgc cggtcgggat gacgttgcgg ttacagacag
      541 ggatgccaaa atcaatgccc cccccccgaa tctgcatacc ggagacttta caaacccaaa
      601 tgacgcatac aagaatttga tcaacctcaa acctgcaatt gaagcaggct atacaggacg
      661 cggggtagag gtaggtatcg tcgacacagg cgaatccgtc ggcagcatat cctttcccga
      721 actgtatggc agaaaagaac acggctataa cgaaaattac aaaaactata cggcgtatat
      781 gcggaaggaa gcgcctgaag acggaggcgg taaagacatt gaagcttctt tcgacgatga
      841 ggccgttata gagactgaag caaagccgac ggatatccgc cacgtaaaag aaatcggaca
      901 catcgatgtg gtctcccata ttattggcgg gcgttccgtg gacggcagac ctgcaggcgg
      961 tattgcgccc gatgcgacgc tacacataat gaatacgcat gatggaacca agaacgaaat
     1021 aatgtctgca gccatccgca atgcatgggt caagctgggc gaacgtggcg tgcgcatcgt
     1081 caataacagt tttggaacaa catcgagggc aggcactgcc gaccatttcc aaatagccaa
     1141 ttcggaggag cagtaccgcc aagcgttgct cgcctattcc ggcggtgata aaacagacga
     1201 gggtatccgc ctgatgcaac agagcaatta cggcaacttg tcctaccaca tccgtaataa
     1261 aaacatgctt ttcatttttt cggcaagcaa tgacgcacaa gctcagccca acacactgac
     1321 cctattgcca ttttatgaaa aagatgctca aaaaggcatt atcacagtcg caggcgtaga
     1381 ccgcagtgga gaaaagttca atggctccaa ccattgcgga attactgcca tgtggtgcct
     1441 atcggcaccc tatgaagcaa gcgtccgttt cacccgtaca aacccgattc aaattgccgg
     1501 aacatccttt tccgcaccca tcgtaaccgg cacggcggct ctgctgctgc agaaataccc
     1561 gtggatgagc aacgacaacc tgcgtaccac gctgctgaca acggctcagg acatcggtgc
     1621 agtcggcgtg gacagcaagt tcggctgggg actgctggat gcgggtaagg ccatgaacgg
     1681 acccgcgtcc tttccgttcg gcgactttac cgccgatacg aaaggtacat ccgatattgc
     1741 ctactccttc cgtaacgaca tttcaggcac gggcggcctg atcaaaaaag gcggcagcca
     1801 actgcaactg cacggcaaca acacctatac gggcaaaacc attatcgaag gcggttcgct
     1861 ggtgttgtac ggcaacaaca aatcggatat gcgcgtcgaa accaaaggtg cgctgattta
     1921 taacggggcg gcatccggcg gtagcctgaa cagcgacggc attgtctatc tggcagatac
     1981 cgaccgatcc ggcgcaaacg aaaccgtgca catcaaaggc gatctgcagc tgggcggcga
     2041 aggtacgctg tacacacgtt tgggcaaact gctgaaagtg gacggtacgg cgatgaccgg
     2101 cggcaagctg tacatgtcgg cacgcggcaa aggggcaggc tatctcaacc gtaccggaca
     2161 acgtgttccc ttcctgagtg ccgccaaaat cgggcgggat tattctttct tcacaaacat
     2221 cgaaaccgac ggtggtctgc tggcttccct cgacagcgtc gaaaaaacag cgggcagtga
     2281 aggcgacacg ctgtcctatt atgtccgtcg cggcaatgcg gcacggactg cttcggcagc
     2341 ggcacattcc gcgcccgccg gtctgaaaca cgccgtagaa cagggcggca gcaatctgga
     2401 aaacctgatg gtcgaactgg atgcctccga atcatccgca acacccgaga cggttgaaac
     2461 tgcggccgcc gaccgcacag atatgccggg catccgcccc tacggcgcaa ctttccgcgc
     2521 agcggcagcc gtacagcatg cgaatgccgc cgacggtgta cgcatcttca acagtctcgc
     2581 cgctaccgtc tatgccgaca gtaccgccgc ccatgccgat atgcagggac gccggctgaa
     2641 agccgtatcg gacgggttgg accacaacgg cacgggtctg cgcgtcatcg cgcaaaccca
     2701 acaggacggt ggaacgtggg aacagggcgg tgttgaaggc aaaatgcgcg gcagtaccca
     2761 aaccgtcggc attgccgcga aaaccggcga aaatacgaca gcagccgcca cactgggcat
     2821 gggacgcagc acatggagcg aaaacagtgc aaatgcaaaa accgacagca ttagtctgtt
     2881 tgcaggcata cggcacgatg cgggcgatat cggctatctc aaaggcctgt tctcctacgg
     2941 acgctacaaa aacagcatca gccgcagcac cggtgcggac gaacatgcgg aaggcagcgt
     3001 caacggcacg ctgatgcagc tgggcgcact gggcggtgtc aacgttccgt ttgccgcaac
     3061 gggagatttg acggtcgaag gcggtctgcg ctacgacctg ctcaaacagg atgcattcgc
     3121 cgaaaaaggc agtgctttgg gctggagcgg caacagcctc actgaaggca cactggtcgg
     3181 actcgcgggt ctgaagctgt cgcaaccctt gagcgataaa gccgtcctgt ttgcaacggc
     3241 gggcgtggaa cgcgacctga acggacgcga ctacacggta acgggcggct ttaccggcgc
     3301 gactgcagca accggcaaga cgggggcacg caatatgccg cacacccgcc tggttgccgg
     3361 tctgggcgcg gatgtcgaat tcggcaacgg ctggaacggc ttggcacgtt acagctacgc
     3421 cggttccaaa cagtacggca accacagcgg acgagtcggc gtaggctacc ggttctgacg
     3481 gacaggaagc agacagccgc aaagatcacg gtctttgcgg ctgtttctta tgaaaagaaa
     3541 accctattcc aattgcctgc ttctattgtt tcaagacttc ttccaaagat tcggcatcaa
     3601 tcagatgtat agcggattaa caaaaatcag gacaaggcgg cgaagccgc
//
LOCUS       DAXHSF010000173         3642 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.173, whole genome shotgun sequence.
ACCESSION   DAXHSF010000173 DAXHSF010000000
VERSION     DAXHSF010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.173"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..188
                     /locus_tag="ACFM8S_001840"
     CDS             <1..188
                     /locus_tag="ACFM8S_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314865.1"
                     /translation="IFVNPLYRHSRKNKKIKNTKLKYRHSRAGGNLGLSVRKLIGKNG
                     FSNPETPDSCFRGNPVF"
     gene            558..929
                     /locus_tag="ACFM8S_001841"
     CDS             558..929
                     /locus_tag="ACFM8S_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314866.1"
                     /translation="MKKIRLLLEFHCFPVWFYDENDEIIENDWCEELLPQEILSDNHL
                     KELCIWLADEFDKLYCDEMGNFDYEKLQDEFSFQTKENEQEFIDKLTEFRTKFTQKCF
                     GFCALQDNLPKIENGKIVSWQ"
     gene            complement(1064..1648)
                     /gene="rnhB"
                     /locus_tag="ACFM8S_001842"
     CDS             complement(1064..1648)
                     /gene="rnhB"
                     /locus_tag="ACFM8S_001842"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFC6314867.1"
                     /translation="MHILTAGVDEAGRGPLVGSVFAAAVILPETFDLPGLTDSKKLSE
                     KKRDALAEMIKNQAAAWHVAAATPEEIASLNILHATMLAMKRAVDGLAVRPEKIFIDG
                     NRIPEHLNIPAEAVVKGDSKIIEISAASVLAKTARDAEMYALAQRHPQYGFDKHKGYG
                     TKQHLEALKQYGVLPEHRRDFAPVRNLLAQQTLF"
     gene            complement(1717..3612)
                     /gene="mnmG"
                     /locus_tag="ACFM8S_001843"
     CDS             complement(1717..3612)
                     /gene="mnmG"
                     /locus_tag="ACFM8S_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFC6314868.1"
                     /translation="MTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLSHNIET
                     LGQMSCNPSIGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRAQA
                     DRILYKAAIREMLENQENLDLFQQAVEDVTLEGERISGVITAMGVEFKARAVVLTAGT
                     FLSGKIHIGLENYEGGRAGDPAAKSLGGRLRELKLPQGRLKTGTPPRIDGRTIDFSQL
                     TEQPGDTPVPVMSVRGNADMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVG
                     PRYCPSIEDKINRFADKDSHQIFLEPEGLTTHEYYPNGISTSLPFDIQIALVRSMKGL
                     ENAHILRPGYAIEYDYFDPRNLKASLETKTIEGLFFAGQINGTTGYEEAAAQGLLAGA
                     NAVQYVRGQDPLLLRREQAYLGVLVDDLITKGVNEPYRMFTSRAEYRLQLREDNADMR
                     LTEDGYKIGLVGEAQWRMFNEKREAVEREIQRLKTTWYTPQKLAEDEQIRVFGQKLSR
                     EANLHDLLRRPNLDYAALMTLEGAMPSENLSAEVIEQVEIQVKYQGYIDRQNEEIDSR
                     RDIETLKLPDGIDYGKVKGLSAEVQQKLNQHKPETVGQASRISGVTPAAVALLMVHLK
                     RGFKDAK"
BASE COUNT          745 a          818 c         1054 g         1025 t
ORIGIN      
        1 tgatttttgt taatccacta tatcgtcatt cccgcaaaaa caaaaaaatc aaaaacacaa
       61 aactgaaata tcgtcattcc cgtgcaggcg ggaatctagg tctgtcggta cggaaactta
      121 tcgggaaaaa cggtttttcc aaccctgaga ctccggattc ctgttttcgc gggaatccgg
      181 ttttttgagt ttcagtcatt tttgataaat tcttgcagct ttgggtttct agattcccgc
      241 ctgcgcggga atgacgaaat ttcagttttt gattttttgt ttttgtggga atgacggttt
      301 ggaagtttcc cgaaattcaa aaaaaaacgg aaaccggacg gactggattc ccgttttcac
      361 gggaatgacg gattttaggt ttctttatct gaaattccta attatttgat gactgcggtt
      421 aaaaatggac aaattgtagg aatgcaacgc acaagaccta tatatgaatt cacctataac
      481 ggcacaaaac accgtgtggc tattgatata ggcaataatg ggtttattgt tggtgccaac
      541 cccaaaagag tagttaaatg aaaaaaataa gattgttact tgaatttcac tgttttccag
      601 tatggtttta tgatgaaaat gatgaaatta ttgaaaacga ttggtgtgaa gaattattac
      661 cacaggaaat tttatcagac aatcatctga aagaattgtg catttggcta gcagatgaat
      721 ttgataaatt atattgtgat gaaatgggta atttcgatta tgaaaagtta caagatgaat
      781 tttcgtttca aacaaaggaa aatgaacaag agttcattga taaattaaca gaattcagga
      841 cgaaattcac acaaaaatgt tttggttttt gtgcgttaca agataattta ccaaaaattg
      901 aaaacggtaa aattgtttct tggcagtgag cgtagcgtac gcaacttgtt gcccacgctt
      961 ttgattttct gtttttcgca ggaatgacgg tttgggttct ttctctttga agttgcgatg
     1021 ctgaaaatgc cgtctgaagt tcagacggca tttttgtgcc ggtttaaaac agcgtctgct
     1081 gcgcgagcag gtttctgacg ggggcgaagt cgcggcggtg ttcgggcagc acgccgtatt
     1141 gtttgagggc ttccagatgc tgcttcgtgc cataaccttt gtgtttgtcg aaaccgtatt
     1201 ggggatggcg ttgcgccagt gcgtacattt ctgcatcgcg tgcggtcttt gccaaaacgg
     1261 atgcggcgga gatttcgatg attttgctgt cgcctttgac gacggcttcg gcagggatgt
     1321 tcaaatgttc gggaatgcgg ttgccgtcga tgaatatttt ttcgggacgc acagccaaac
     1381 cgtcaacggc gcgtttcatc gcgagcatgg tggcgtgcag gatgttgagg ctggcgattt
     1441 cttcgggcgt ggcggcggcg acgtgccacg cggccgcctg attttttatc atttcggcaa
     1501 gcgcgtcgcg ttttttctcg ctgagttttt tggagtcggt cagtccgggc aggtcaaatg
     1561 tttccggaag aatgacggcg gcggcaaaca cgctgccgac taaaggtccg cgtccggcct
     1621 cgtccacgcc ggcggtcagt atgtgcatga tgtttcctgt tgggatggta ggaatgccgt
     1681 ctgaacgatg tttcagacgg catcgtaccg atgtgtttat ttcgcgtctt taaatccgcg
     1741 cttcaaatgc accatcagca atgccactgc cgcaggtgtt acgccggaaa tgcggcttgc
     1801 ctgcccgacg gtttcgggtt tgtgctggtt gagcttttgc tgcacttctg ccgacaagcc
     1861 tttgactttg ccgtaatcga tgccgtcggg cagttttaag gtttcgatgt cgcggcggct
     1921 gtcgatttct tcgttttggc ggtcgatata gccttggtat ttgacttgga tttcgacttg
     1981 ttcgatgact tcggcggaga ggttttcaga cggcatcgcg ccttcgagcg tcatcagcgc
     2041 ggcgtagtcg aggttcgggc ggcgtaacaa atcgtgcagg ttggcttcgc ggctgagttt
     2101 ttgtccgaac acgcggattt gttcgtcttc ggcgagtttt tgcggcgtgt accacgttgt
     2161 tttcaaacgt tggatttcgc gttcgacggc ttcgcgtttt tcgttgaaca tgcgccattg
     2221 cgcttcaccc accaagccga ttttgtagcc gtcttcggtc aggcgcatat cggcgttgtc
     2281 ttccctgagt tgcaggcggt attcggcgcg gctggtgaac attcggtaag gttcgttcac
     2341 gcctttggtg atgaggtcgt ccaccaatac gccgaggtag gcttgttcgc ggcgcagcag
     2401 gagcgggtct tgtccgcgca catattgcac ggcgttcgcg cctgccaata aaccttgcgc
     2461 ggcggcttct tcgtagccgg tcgtaccgtt gatttgcccg gcgaaaaaca gtccctcaat
     2521 ggttttggtt tcgaggcttg ctttgaggtt gcgcggatcg aagtagtcgt attcgatggc
     2581 gtaaccgggg cgcaggatat gggcgttttc caaacctttc atgctgcgga cgagggcgat
     2641 ttggatgtcg aacggcaggc tggtggagat accgttgggg tagtattcgt gcgtggtcag
     2701 gccttcgggt tcgaggaaaa tctggtggct gtctttatcg gcgaagcggt tgattttgtc
     2761 ttcgatagac ggacaataac gcgggcccac gccttcgatt ttgccggtaa acatcgggct
     2821 gcggtcgaag cctgagcgga tgatgtcgtg ggtttgcgtg ttggtatgcg taatccagca
     2881 ggacacttgg cgcgggtgca tatcggcgtt gccgcgcacg gacatgaccg gaacgggcgt
     2941 gtcgccgggc tgttcggtca gttgggagaa gtcaatcgtg cgtccgtcaa tacgcggcgg
     3001 cgtgccggtt ttcagacggc cttgcggcag cttcaattcg cgcaaacgtc cgcccaacga
     3061 tttggcggcg gggtcgccgg cgcgtccgcc ttcgtagttt tccaaaccga tgtggatttt
     3121 gccggacaaa aacgtgcctg cggtcagcac cacggcgcgt gctttaaact ccacgcccat
     3181 cgcggtgatt acgccgctga tgcgttcgcc ctcgagcgtt acgtcttcga cggcttgttg
     3241 gaaaaggtcg aggttttctt ggttttccaa catttcgcgg atggcggctt tatacaaaat
     3301 gcggtcggcc tgtgcgcgcg tggctcgtac tgccgcgcct ttgctggcgt tcagacggcg
     3361 gaactggatg ccggatttgt cggttgccaa cgccatcgcg ccgccgagcg cgtcgagttc
     3421 gcgcaccaaa tgccctttgc cgatgccgcc gatggaaggg ttgcacgaca tttgtccgag
     3481 cgtttcgata ttgtgtgaga gcaaaagcgt ctgcgcgccc atgcgtgcgg cggcaagcgc
     3541 ggcttccgtg ccggcgtgtc cgccgccgac gacgataacg tcgtaggttt tggggtaaat
     3601 catgtgggtc atgtgtgtat tgcctgtcgg tgtttcagac gg
//
LOCUS       DAXHSF010000174         3631 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.174, whole genome shotgun sequence.
ACCESSION   DAXHSF010000174 DAXHSF010000000
VERSION     DAXHSF010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.174"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..486
                     /gene="mafB"
                     /locus_tag="ACFM8S_001844"
     CDS             <1..486
                     /gene="mafB"
                     /locus_tag="ACFM8S_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HFC6314869.1"
                     /translation="GDFSAAYNTRTTRKVTTETEGLNRIRQNQKNSNIHEKNYGRDNP
                     NHINVLSGNSIQHILYGDEAGGGHLFPGKPGKTTFPQHWSASKITHEISDIVTSPKTQ
                     WYAQTGTGGKYIAKGRPARWVSYETRDGIRIRTVYEPATGKVVTAFPDRTSNPKYNPV
                     K"
     gene            501..731
                     /locus_tag="ACFM8S_001845"
     CDS             501..731
                     /locus_tag="ACFM8S_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HFC6314870.1"
                     /translation="MKTLDERIKNLGKSLEDRIDANLIDAALEYITFSERLLAFETLC
                     DYIEDFNIQLTEKESQEISFINKEFGIESTSD"
     gene            880..1578
                     /locus_tag="ACFM8S_001846"
     CDS             880..1578
                     /locus_tag="ACFM8S_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HFC6314871.1"
                     /translation="MVKTADGYKAIAHIQAGDRVLSKDEASGETGYKPVTARYGNPYQ
                     ETVYIEISDGIGNNQTLISNRIHPFYSGGKWIKAEDLKAGSRLLSESGKTQTVRNIVV
                     KPKPLKAYNLTVADWHTYFVKGSQAETEGVWVHNSCPPYKRPNNATTKAQRESVQGKP
                     CVECGKLAEKMIADHKKPLVVEYYETGTIDKSKMRAIESVQPQCPTCSAKQGGRLSQY
                     SKEQKRKLSNGNKK"
     gene            1562..1915
                     /locus_tag="ACFM8S_001847"
     CDS             1562..1915
                     /locus_tag="ACFM8S_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314872.1"
                     /translation="MGIKNEQIIICKKYNTEIYPVSDVSKIGVAENVKQTGLYPINGL
                     RHRPKGDTNGWYIWAGENFSYDKNFFLPLHTFHLQIWRPEIIPFLTLPPGYRFLIGEN
                     GYEDVWFDELLLNDN"
     gene            1905..2228
                     /locus_tag="ACFM8S_001848"
     CDS             1905..2228
                     /locus_tag="ACFM8S_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFC6314873.1"
                     /translation="MIIDKKSTSDVLKQYQELGFDLTKPMIIEFFIGGSQKNLQSIES
                     RLISYRQDFQISIEQDEFGEMWTCYCSVNIVPSLENILAIETTLFGIAQKNDCNYEGF
                     GSYGN"
     gene            2287..2514
                     /locus_tag="ACFM8S_001849"
     CDS             2287..2514
                     /locus_tag="ACFM8S_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314874.1"
                     /translation="MDIKFNTLGVILNGVNPEEKFIKIIDDQENTGGFLILLSSNDKF
                     SSFDSYDDWVENLEILKEYLQESHWIIKWVG"
     gene            2642..3184
                     /locus_tag="ACFM8S_001850"
     CDS             2642..3184
                     /locus_tag="ACFM8S_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HFC6314875.1"
                     /translation="MINHIEKYTGTISHGSKSDSGSQYKLNIAAIPSSPNRDLKTYIT
                     LGLSKHDLNYKSRSEILFVCSLKYDENQIFPFLRWLAETIIENKKILLRGQVVYLPRS
                     IVDSTKMDALYVSAPFYFDDDFQVCYGEHYNIVFPLLVPLYKQEAEWVEKKGWNAFEQ
                     FLLDNEVGNLSDMNRKPFTW"
     gene            3291..3631
                     /locus_tag="ACFM8S_001851"
                     /pseudo
     CDS             3291..3631
                     /locus_tag="ACFM8S_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951299.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin-type HINT domain-containing
                     protein"
BASE COUNT         1237 a          692 c          730 g          972 t
ORIGIN      
        1 ggggattttt ctgctgcata caatacaaga acaactagaa aagttactac agaaacagag
       61 gggttaaata gaatcagaca gaaccagaaa aatagtaata tacatgagaa aaattatgga
      121 agagataatc ctaatcatat taatgtttta tctggaaatt ctatacaaca tatactgtat
      181 ggagatgaag caggaggtgg gcatcttttt cctggcaaac ctggtaagac aacattcccc
      241 caacattggt cagccagtaa aataactcat gaaattagtg atatcgttac atccccaaaa
      301 acgcaatggt atgcacagac tggaacaggc ggcaaatata ttgctaaagg aagaccagcc
      361 aggtgggtat catatgaaac gagagatgga attcgtatca gaacagttta tgaacctgca
      421 acaggaaaag tggtaactgc attccccgat agaacctcta atcccaaata taaccctgta
      481 aaataatgag tatttgaaag atgaaaacat tagacgaacg aataaaaaat ttaggaaaat
      541 ctcttgaaga tagaattgac gctaatctaa ttgatgccgc actagagtac attacttttt
      601 cagaacgttt gctagctttt gaaacgctat gtgattatat agaagatttc aatatccagc
      661 ttacggaaaa agaatcccaa gaaatctctt ttattaataa ggaatttgga attgaaagta
      721 catcagatta ataaatatta ggtcggttta aaaacccaac aatcaaaaag tcgtctgaaa
      781 ctgtgtttaa tttacagacg acctttcctt catttgaagc aggatattga gaactgagtt
      841 cttcaaaaat cctacacctg ctccttccac ggcagcacct tggtcaaaac ggcagacggc
      901 tacaaagcca ttgcccatat tcaagccggc gaccgcgtcc tttccaagga cgaggcaagc
      961 ggagaaacgg gatacaaacc tgttaccgcc cgatacggca atccgtatca agaaaccgtt
     1021 tacattgaaa tttcagacgg catcggcaac aaccaaaccc tgatttccaa ccgcatccat
     1081 ccgttttatt cgggcggcaa atggattaag gcggaagact taaaggcggg aagcaggctg
     1141 ttatccgaaa gcggcaaaac ccaaaccgtc cgcaacatcg ttgtcaaacc aaaaccgctc
     1201 aaagcctaca atctgaccgt tgccgactgg catacttact tcgtcaaggg cagtcaggcg
     1261 gaaacggaag gggtttgggt tcataattct tgtccgccat ataagagacc aaataacgca
     1321 acgacaaaag cgcaacgaga aagtgtacaa ggaaaaccct gcgttgaatg cggtaaatta
     1381 gccgaaaaaa tgattgccga ccataaaaaa ccattagttg tcgagtatta tgaaacaggg
     1441 acaattgaca aatctaagat gagagcaatt gaatcagtac aaccgcaatg tccgacttgt
     1501 tctgcaaaac aaggagggag attaagccaa tattcaaaag aacagaaaag gaaactgagt
     1561 aatgggaata aaaaatgaac aaataataat ttgtaaaaaa tataatacag aaatatatcc
     1621 tgtttccgat gtttcaaaaa tcggtgttgc tgaaaatgtc aaacagacag gcctgtatcc
     1681 gattaacggg ttaaggcacc gtcccaaagg tgatactaac ggttggtata tctgggccgg
     1741 tgaaaacttc tcctatgata aaaatttttt cctgcccttg catacgttcc atttgcaaat
     1801 atggcgaccc gaaatcatcc cctttttaac acttccgccc ggttaccgtt ttttaattgg
     1861 ggaaaacggt tatgaagatg tatggtttga tgaattgcta ttaaatgata attgacaaaa
     1921 aatctacttc agatgtactg aaacaatatc aagaactagg gtttgatttg acaaaaccta
     1981 tgataataga gttttttata ggaggttctc aaaagaatct gcaatcaata gaaagccggc
     2041 tcatttctta tcggcaagat tttcaaatca gtattgaaca agatgaattc ggagaaatgt
     2101 ggacttgcta ttgttccgtc aatatcgtcc cttcccttga aaacatttta gctattgaaa
     2161 ctacgttatt tggtattgca caaaaaaatg attgtaatta tgaaggtttt ggttcgtatg
     2221 ggaactaaca aaaagtgtag gttgggttga aaaaaccata ttcccattaa agattagggt
     2281 gtgtctatgg acataaaatt caatactttg ggggttatcc tgaatggggt caatcctgaa
     2341 gaaaaattca taaaaattat tgatgatcag gaaaataccg ggggatttct gatactgtta
     2401 tcttcaaatg acaaattctc atcatttgac agttacgatg actgggtcga aaacttagaa
     2461 atattaaaag aatacctgca agaatctcat tggataataa agtgggtcgg atagtcggca
     2521 aacgtagtcc gtctgaaacg cttttacttt tcagacggac tttctatcaa tgggaacaag
     2581 gcgttttgtc tcaatctggt ctatgattat ttcgatacag tgatgggaag ctaagaaaaa
     2641 tatgattaat catattgaaa aatatacagg cactatttct catggctcga aaagtgattc
     2701 gggaagtcag tacaaattaa atatcgcagc tattccctct tcccctaaca gggatttaaa
     2761 aacttatatt actttagggt taagcaaaca tgatctgaat tataaaagca gatctgaaat
     2821 actttttgta tgttctttaa aatatgatga aaatcagatt tttccatttt taagatggtt
     2881 ggcagaaacc atcattgaga ataaaaaaat ccttcttcgg gggcaggttg tttatttgcc
     2941 cagaagcatt gttgattcaa caaaaatgga tgcactgtat gtttccgccc cattttattt
     3001 cgacgatgat tttcaggtct gttatggcga acactacaat atcgttttcc ctttgcttgt
     3061 ccccttgtat aaacaggaag ccgaatgggt ggaaaaaaag ggttggaatg cttttgagca
     3121 gttcttgttg gataatgaag ttggcaacct ttcggatatg aatcggaaac cgtttacttg
     3181 gtaaggattt tcttaaattt gattaaaggt cgtctgaaac cgtgtttaac tttcagacaa
     3241 cctttccttc atttgaaaca ggatattgaa aactaagttt ttcaacattc tacacctgct
     3301 ccttccacgg cagcaccttg gtcagaacgg cagacggcta caaagccatt gcccatattc
     3361 aagccggcga ccgcgtcctt tccaaggacg aggcaagcgg agaaacggga tacaaacctg
     3421 ttaccgcccg atacggcaat ccgtatcaag aaaccgttta cattgaaatt tcagacggca
     3481 tcggcaacaa ccaaaccctg atttccaacc gcatccaccc gttttattcg gacggcaaat
     3541 ggattaaagc ggaagatttg aaagcgggaa gcaggctgtt tgccgaaagc ggcaaaaccc
     3601 aaaccgtccg caacaccgtt gtcaaaccaa a
//
LOCUS       DAXHSF010000175         3466 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.175, whole genome shotgun sequence.
ACCESSION   DAXHSF010000175 DAXHSF010000000
VERSION     DAXHSF010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.175"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(198..578)
                     /locus_tag="ACFM8S_001852"
     CDS             complement(198..578)
                     /locus_tag="ACFM8S_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HFC6314876.1"
                     /translation="MNIRFFALTVPVLSLAACAVPEAYDDGGRGHMSSVQNQARPADF
                     RAFSCENGLSVRVRHLDSGKVALRLDGRRAVLSSDVAASGERYTAEHGLFGNATEWHQ
                     KGGEAFFGFTDAYGNSVETSCRAR"
     gene            1054..3135
                     /locus_tag="ACFM8S_001853"
     CDS             1054..3135
                     /locus_tag="ACFM8S_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HFC6314877.1"
                     /translation="MKSLKTFLIWGIVVLVGLASFTTLALSRGEQVSAVWMVTAAISV
                     YCIAYRFYSLYIANRVMRLDPNRLTPAERHNDGLDYVPTHKGVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGTLWIIFGVVFAGAVQDMMVLFVSMRRDGKSLGDIVKQELGTVPG
                     VIASIGILMIMVIIMAVLALIVVKALVHSPWGTFTIAATMPIALFMGIYTRYIRPGKI
                     GEISIVGFILLMLAVIYGEDVAKSSIGHWFDLDGIQLTWAIMIYGFVASVLPVWLLLT
                     PRDYLSTFLKIGTIAALALGIVIVNPTLQMPAVTHFIDGSGPVFSGALFPFLFITIAC
                     GAVSGFHALISSGTTPKMLENETHVRMIGYGGMLMESFVAIMALAAAASLDPGVYFAM
                     NSPAALIGTDANTAAEVITTKLQFPVDAATLLHTAKEVGENTILSRAGGAPTLAVGMA
                     HIMSRLIPGEAMMAFWYHFALLFEALFILTAVDAGTRVARFMIQDLGSIFYKPFGNTD
                     SIPANLIATFFAVALWGYFLYTGVTDPLGGINSLWPLFGIANQMLAGVALIMCAVVLI
                     KMKRDRYVWVVLVPAVGVLFVTCYAGLQKLFHSDPRISFLAHAGKYSDALAKNEVLAP
                     AKDIGEMAQIIFNDKINAGLTILFLSVVVIVAAYGLRTALKARKVGWPTAKEIPAVYR
                     DGKQPEAQSEA"
     gene            3125..3319
                     /locus_tag="ACFM8S_001854"
     CDS             3125..3319
                     /locus_tag="ACFM8S_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFC6314878.1"
                     /translation="MKHKLASWWKTIKLTANLMAGVPDYENYVAQQRKHNPNAPVMTK
                     LQFQDYCRKRRCGANGGRCC"
BASE COUNT          777 a         1022 c          876 g          791 t
ORIGIN      
        1 cattggataa cagccctgaa gaaatcggag caggtttaaa gttggcattg agccgctgcc
       61 gataggataa acccggaacg aaacgaaaaa taatccgcca cgcccgaaaa ccgccgaaac
      121 cagccgacag ccaaaacaaa tccgacacaa aaaaccgccc gaaaaatccg gacggcggtt
      181 caaacaggct gccccgttta acgggcgcgg caggaagttt cgaccgaatt gccgtaggca
      241 tcggtaaagc cgaaaaaggc ttcgccgcct ttctggtgcc actcggttgc gtttccgaac
      301 aaaccgtgtt cggcggtata gcgttcgccg gatgcggcaa cgtcggaaga gaggacggca
      361 cgcctgccgt ccagccgcaa cgcgactttg ccgctgtcca aatggcggac gcgcacagac
      421 aaaccgttct cgcatgaaaa cgcccgaaaa tctgccgggc gggcttggtt ttgaacggac
      481 gacatatgcc ctcgtccgcc gtcatcatac gcctccggca cggcacaggc cgccaaagac
      541 aaaaccggta cggtcagcgc gaaaaacctg atattcataa aagctcccca ataaaaataa
      601 gatatgaaac aaccgccctg attccaagct gcggcaacgc catactataa acggacgcgc
      661 aaacacacaa gcccgataac cggaatttac ccgcgatgaa tcaataatcc ggattgcgcg
      721 ccccttcttt accggtcttc cggcgccgcc ttttgcctga cgatgccgtc caaacccgcc
      781 tgtccgcccg aaggaatgta aattttttcc aaattccaag taaaaaccgc tatcagtgtg
      841 ctaatttgcg ttaaaatcct attcggcgtt taacgttttg tgcgcccgca tccctgcact
      901 gtttgatgcg ggcataaggc acaaatcccg acaagcgcac tgtttcatac ttcgtcaatc
      961 attcagactc cggtttgtgc ccgtgccggc aggcggtcat ccgtttcccg ccgttcaggc
     1021 atattccgac agtgtgagat aaggatttat tcgatgaaat cactcaaaac cttcctcatt
     1081 tggggcatag tggtactggt cggcttagca tcctttacca ctctggctct cagccgaggc
     1141 gaacaggtca gcgcggtatg gatggttacc gccgccatat ccgtttactg catcgcctac
     1201 cgtttttaca gcctctacat cgccaaccgc gtaatgcggc tcgatcctaa ccgcctgact
     1261 ccggcagaac gccacaacga cggcttggac tacgttccga cgcacaaggg cgtattgttc
     1321 ggacaccact ttgccgcgat tgccggcgcg ggccccttgg tcggcccggt tttggcggcg
     1381 caaatgggtt acctgcccgg tactttgtgg attatcttcg gcgtggtgtt tgccggcgcg
     1441 gtacaggata tgatggtctt gttcgtctct atgcgccgcg acggtaagtc tttgggcgat
     1501 attgtgaaac aggaactcgg cactgtcccc ggcgtgattg cctccatcgg tattttgatg
     1561 attatggtca tcattatggc ggtgttggcg ttgattgtcg taaaagcatt ggttcacagc
     1621 ccttggggta cgtttaccat cgccgccact atgccgattg ccctgtttat gggtatttac
     1681 acgcgctata tccgtccggg caaaatcggc gagatttcca tcgtcggctt tattttgctg
     1741 atgctggcgg taatttacgg cgaagatgtg gcgaaaagtt ccatcggaca ttggttcgac
     1801 cttgacggca tccagctcac ttgggcgatt atgatttacg gctttgtcgc ctccgtattg
     1861 cccgtatggc tgctgctcac tccgcgcgac tatctctcca ccttcctgaa aatcggtacg
     1921 attgcggcct tggctttggg catcgtcatc gtcaatccca ctttgcaaat gcctgccgta
     1981 acccacttta tcgacggttc cggcccggta ttctcaggcg cattgttccc gttcctcttc
     2041 atcaccatcg cctgcggtgc ggtttcgggc ttccacgcgc tgatttcctc cggcaccacg
     2101 ccgaaaatgc tggaaaacga aacccacgtc cgtatgattg gttacggcgg tatgttgatg
     2161 gaaagtttcg tagccattat ggcacttgcc gccgccgcat cgcttgatcc cggcgtgtac
     2221 ttcgccatga acagccctgc cgccctgatc ggtacggatg ccaataccgc cgccgaagtg
     2281 attaccacca agctgcaatt ccctgtcgat gctgcaaccc tgttgcacac cgctaaggaa
     2341 gtgggcgaaa acaccatcct ttcccgtgca ggcggtgcgc ccaccctcgc agtcggtatg
     2401 gcgcacatta tgagccgcct gattccgggc gaggcgatga tggcgttctg gtatcacttc
     2461 gccctattgt ttgaagcctt gttcatcctg accgccgtcg atgccggtac gcgcgtcgca
     2521 cgttttatga ttcaagactt gggcagcatc ttctacaaac ctttcggcaa caccgactcc
     2581 atccccgcca acctgattgc gaccttcttc gccgtggcat tgtggggcta ctttctctac
     2641 accggcgtaa ccgacccgct gggcggcatc aactcgctct ggcctttgtt cggcatcgcc
     2701 aaccaaatgc tggcaggcgt agccttgatt atgtgcgccg tggtgctgat taagatgaaa
     2761 cgcgaccgtt atgtctgggt ggtactcgtt cccgccgtcg gcgtactgtt cgtaacctgc
     2821 tacgccggcc tgcaaaaact gttccacagc gacccgcgca tcagcttcct tgcccacgcc
     2881 ggcaaataca gcgacgcatt ggctaaaaac gaagtccttg cgcctgccaa agacatcggc
     2941 gaaatggcgc aaatcatctt caacgacaag attaatgccg gtctgaccat cctcttcttg
     3001 tcggttgtcg tgattgtcgc cgcgtacggt ttgcgtaccg ccctcaaagc acgcaaagtc
     3061 ggctggccga ccgccaaaga aatcccggcg gtgtaccgcg acggcaagca gccggaggca
     3121 caaagtgaag cataagctcg cgtcttggtg gaaaaccatc aagctgacgg caaacttgat
     3181 ggcaggcgtg cctgattatg aaaactacgt tgcacagcag cgcaaacata atcccaacgc
     3241 ccccgtgatg accaagctgc aatttcaaga ctattgccgc aaacgccgct gcggcgcaaa
     3301 cggcggacgc tgctgttaag cctgcttgaa acaaattccg tctgaacgcc gcttcagacg
     3361 gaatttttat aatatagtgg attaacaaaa atcaggacaa ggcgacgaag ccgcagacag
     3421 tacaaatagt acgaaaccga ttcactcggt gcttcagcac cttaga
//
LOCUS       DAXHSF010000176         3442 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.176, whole genome shotgun sequence.
ACCESSION   DAXHSF010000176 DAXHSF010000000
VERSION     DAXHSF010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.176"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(268..906)
                     /locus_tag="ACFM8S_001855"
     CDS             complement(268..906)
                     /locus_tag="ACFM8S_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HFC6314879.1"
                     /translation="MFQNFDLGVFLLAVLPVLLAITVREVARGYTARYWGDNTAEQYG
                     RLTLNPLPHIDLVGTIIVPLLTLMFTPFLFGWARPIPIDSRNFRNPRLAWRCVAASGP
                     LSNLAMAVLWGVVLVLTPYVGGAYQMPLAQMANYGILINAILFALNIIPILPWDGGIF
                     IDTFLSAKYSQAFRKIEPYGTWIILLLMLTGVLGAFIAPIVRLVIAFVQMFV"
     gene            1161..2003
                     /locus_tag="ACFM8S_001856"
     CDS             1161..2003
                     /locus_tag="ACFM8S_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HFC6314880.1"
                     /translation="MDKERILTPAVVFSVALLHLAMVVLLWQAHKLPVIESGNVIEFV
                     DLGDFGGGDGAPEGAGAPAAPEPQPEPEPPKPVEPPKPVLKPVVTKKADADIQQPKEE
                     PKPEEKPKPEEKPKPEPKPEAKPVPKPAEKPVEKPSEKPAEHPSNASAKADSEQGNGE
                     DKGTGTKGDGTGRGEGSGKGSGGVKGEHGEGAGSSKGNPLRANGSIPRPAYPTLSMEN
                     DEQGTVVLSVLVSPGGHVESVKIVKSSGFSRLDNAARKAAQNGHFQANAWTEFKVPVK
                     FELN"
     gene            2069..2731
                     /gene="exbB"
                     /locus_tag="ACFM8S_001857"
     CDS             2069..2731
                     /gene="exbB"
                     /locus_tag="ACFM8S_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HFC6314881.1"
                     /translation="MNLKLVFESGDPVLIGVFVLMLLMSIVTWCLVVLRCIKLYRARK
                     GNAAVKRHMRDTLSLNDAVEKVRAVDAPLSKLAQEALQSYRNYRRNEASELAQALPLN
                     EYLVIQIRNSMAQIMRRFDYGMTALASIGATAPFIGLFGTVWGIYHALINIGQSGQMS
                     IAAVAGPIGEALVATAAGLFVAIPAVLAYNFLNRGTKILTQDLDAMAHDLHVRLLNQK
                     DS"
     gene            2734..3168
                     /locus_tag="ACFM8S_001858"
     CDS             2734..3168
                     /locus_tag="ACFM8S_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HFC6314882.1"
                     /translation="MAFGSMNSGDDSPMSDINVTPLVDVMLVLLIVFMITMPVLTHSI
                     PLELPTASEQTNKQDKQPKDPLRLTIDANGGYYVGGDSASKVEIGEVESRLKAAKEQN
                     ENVIVAIAADKAVEYDYVNKALEAARQAGITKIGFVTETKAQ"
BASE COUNT          906 a          799 c          973 g          764 t
ORIGIN      
        1 gggtttcggc tgtcgggagg gaaaggaatt ttgcaaaggt ctcagacggc aataaatcca
       61 gcatttgatt ggcaagtttt ggcgatgtga aaacctcatc attagacaga ctggagtttc
      121 ttaatcagtg tttcagttaa tgaaaggtat tatcatgcaa cttggttcta ataatgatga
      181 aaaaaataag attacactac aggaaaacgc ggcagggcaa tatgtcctgc cgcgttttct
      241 ggagcgttta tgccgtctga aagccagtca gacgaacatc tgcacaaacg caatcaccag
      301 ccgcacaatc ggtgcaataa acgcacccaa aaccccggtc agcatcagca gtaggataat
      361 ccacgtccca taaggttcga ttttgcggaa cgcttgcgaa tatttcgccg acaggaaggt
      421 gtcgatgaaa atgccgccgt cccaaggcag gatggggatg atgttgagcg cgaacagaat
      481 cgcattgatc agaataccgt agtttgccat ttgagccaac ggcatctgat acgccccgcc
      541 gacatacgga gtcagcacca aaaccacgcc ccacagaaca gccatcgcca gattcgacag
      601 cgggccggac gcggcaacgc aacgccaggc aaggcgcggg ttgcggaagt tgcgcgaatc
      661 gataggaatc ggacgcgccc agccgaacag gaagggcgtg aacatcaaag taagcagcgg
      721 tacgatgatt gtgccgacca aatcgatatg gggcaggggg ttcagtgtca gcctgccgta
      781 ttgttcggca gtgttgtctc cccagtagcg cgccgtatag ccgcgcgcca cctccctgac
      841 ggtaatggcg agcagcacgg gcaggacggc aagcagaaac acgcccaaat caaaattttg
      901 aaacatggtt ttcctttgaa cagatgtggt gtgcagcccg aaacggctga cagacagaag
      961 cggcaatata tcatctaacc tgattttatt ggaataagaa atcagacgga atcatttttt
     1021 ctgccggccc gttatctgat gatttgctgt aatacagcgt ttaaacacgg tttgcctgtg
     1081 aacggaacag acattcatat gaccaaaaat tgtaaaaata ggttgcaaat aggaattgtt
     1141 gctattatta tgcccgaatt atggataaag aacgaatttt aacccccgca gtcgtgtttt
     1201 ccgtcgcact gctgcatttg gcaatggtgg tattgctctg gcaggcgcac aagctgcccg
     1261 tgatagagtc aggaaatgtt atcgaatttg tcgatttggg tgattttggc ggaggggacg
     1321 gtgcacccga aggcgcaggt gcgcctgctg cgcctgagcc gcaacctgag cctgagccgc
     1381 ccaaacctgt cgagccgccc aagccggttt tgaagccggt ggttacgaaa aaggcggatg
     1441 cggatattca gcagcctaag gaagagccga aacctgaaga aaagccgaaa cccgaagaaa
     1501 aaccgaaacc agagcctaag ccggaagcga agcctgtccc gaaaccggcg gaaaaaccgg
     1561 tcgagaagcc gtctgaaaaa cctgccgaac atcccagcaa tgcttctgcc aaagcagaca
     1621 gcgagcaggg taatggggaa gataagggaa ccggtaccaa aggagacgga acggggcgcg
     1681 gagaaggcag cggtaaaggt agcggcggtg tcaaaggcga acacggggaa ggagccggca
     1741 gcagcaaagg caatccttta cgcgccaacg gcagcattcc gcgcccggct tatcccacgc
     1801 tttctatgga gaatgacgag cagggtacgg ttgttttgag cgttttggtt tctccgggcg
     1861 gtcatgtcga gtccgttaaa atcgtgaaaa gcagtggttt ttcccggttg gacaatgcgg
     1921 cacgcaaggc ggcgcaaaac gggcattttc aagccaatgc ctggacggag tttaaagtcc
     1981 ccgtcaagtt tgaattgaat taggcggttg ccgcagcggt ttgcggcagc ttggatgttt
     2041 gattggtttt ttcagtgttg gaatcattat gaatttgaaa ttagtatttg aatcgggcga
     2101 tcccgtcctg atcggtgtgt ttgtgttgat gctgttgatg agtatcgtaa cgtggtgttt
     2161 ggttgtcttg cgctgcatca agctgtatcg ggcgcgcaaa gggaatgccg ccgtcaaacg
     2221 gcatatgcgc gatactttgt cgctgaacga cgcggtcgaa aaagtgcgcg ccgtcgatgc
     2281 gcctttgtcc aaactggcgc aagaggcatt gcagtcttac cgcaactacc gccgaaacga
     2341 agcgtccgaa ctggcgcagg ctttgccgtt gaacgagtat ttggtcattc aaatccgcaa
     2401 cagtatggcg cagattatgc gccggtttga ttacgggatg accgcgcttg cctccatcgg
     2461 cgcgaccgcg ccgtttatcg ggctgttcgg cacggtttgg gggatttacc acgccctgat
     2521 caatatcggg caaagcgggc agatgagtat tgcggcggtt gccggcccga ttggcgaggc
     2581 actggtggcg acggcggcgg gtttgttcgt ggcgattccg gcggtgttgg catacaactt
     2641 cctcaatcgc ggcacaaaaa tactgaccca ggatttggat gcgatggcgc acgatttgca
     2701 cgtccgcctg cttaatcaaa aggatagctg attatggcat ttggttcgat gaattccggc
     2761 gacgattctc cgatgtccga catcaacgtt acgccgttgg tggacgtgat gctggtgttg
     2821 ctgattgtgt ttatgattac tatgccggtg ctgacgcatt ccatcccttt ggaactgccg
     2881 accgcgtccg agcagacaaa caagcaggac aaacagccta aagaccccct gcgcctgacg
     2941 attgatgcga acggcggcta ttatgtcggc ggggattctg caagcaaagt ggaaatcggg
     3001 gaagtggaaa gccgtctgaa agccgccaag gagcagaatg aaaacgtgat tgtggcgatt
     3061 gcggcagaca aggcggtgga atacgattat gtaaacaaag ctttagaagc cgcccgtcag
     3121 gcaggaatca ccaaaatcgg ttttgtaacc gaaaccaagg cgcaataaag ttcgacgctt
     3181 gaacgaatgc cgtctgaaag tctttcagac ggcatttctt atgtttaatc agctatatat
     3241 agttgattaa aacaaaaatg agacgacaga gcgccgtctc catttctgcg caggcgggaa
     3301 tacaaacatc tgatttttct tttattttca aacattactg aaatgggtaa gtctggagtc
     3361 ctgcctgcgc gagaataatg aaaatgtgca tttctaattt ttatagtgga ttaacaaaaa
     3421 tcaggacaag gcaacgaagc cg
//
LOCUS       DAXHSF010000177         3418 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.177, whole genome shotgun sequence.
ACCESSION   DAXHSF010000177 DAXHSF010000000
VERSION     DAXHSF010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.177"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            43..717
                     /locus_tag="ACFM8S_001859"
     CDS             43..717
                     /locus_tag="ACFM8S_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HFC6314883.1"
                     /translation="MCTRKQQGFTLTELLIVMVIAAIMAMIALPNMSQWIASRRIASH
                     AERIANLLRFSRGEAVRLNLPVYICPVQVKKDGAPNNRCDFSKKGRGMLAFGDKNGNK
                     AYDGDAADVFLRSVVLNDTDDSRINYAFNHIAFGSSQPTADRVVWTFNQNGTFGYTKD
                     QHLTRKSSFFYSDGYIQIVLTDARAVSDADKKFRSAVVLIDSSGRVEVCPRGDRRTRR
                     TMCQYK"
     gene            741..1355
                     /gene="pilV"
                     /locus_tag="ACFM8S_001860"
     CDS             741..1355
                     /gene="pilV"
                     /locus_tag="ACFM8S_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HFC6314884.1"
                     /translation="MKNNDCFRLKDSQSGMALIEVLVAMLVLTIGILALLSVQLRTVA
                     SVREAETQTIVSQITQNLMEGMLMNPTIDSDSNKKNYNLYMGNHHTLSAVDGDFAVDA
                     VKSKKDLAKAQLDRFGYELKNALPDAAAIHYAICKDSSGAAPTLSAGGAFSQNCDGSA
                     NGDTLIKVLWVNDSAGDSDITRTNLETNGDNIVYTYQARVGGRE"
     gene            1352..2290
                     /locus_tag="ACFM8S_001861"
     CDS             1352..2290
                     /locus_tag="ACFM8S_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HFC6314885.1"
                     /translation="MRRKMLNVPKGNYDGMKGFTIIEFLVAGMLSMIVLMAVGSSYFT
                     SRKLNDAANERLAAQQDLRNAATLIVRDARMAGGFGCFNMSEHPATDVVSDVAQKNHL
                     FSLKRNSTNKLIPIAESSNIRYPGFTQRLNALIFQYGIDDVNASADTTVVSSCAKIAK
                     PGKKISTLQEAKSVLQITNDDKQNGNITRQRHVVNAYAVGRIAGEEGLFRFQLNDDGQ
                     WGNPQLLVKKINKMDIRYIYVSGCPEDDDAGKEETFRYTDKFNSAQDAVTPAGVEVLL
                     SSGTDTKIAASSDNHIYAYRIDATIRGGNVCANRTL"
     gene            2269..2865
                     /locus_tag="ACFM8S_001862"
     CDS             2269..2865
                     /locus_tag="ACFM8S_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HFC6314886.1"
                     /translation="MRKQNTLTGIPTSDGQRGFALFIVLMVMIVVAFLVVTAAQSYNT
                     EQRISANESDRKLALSLAEAALREGELQVLDLEYDTDSKVTFSENCEKGLCTAVNVRT
                     NNTGNEEAFDNIVVQGKPTVEAVKRSCTAKSTGLCIDKKGMEYKKGKGSVSKSPRYII
                     EYLGVKNGENVYRVTAKAWGKNANTVVVLQSYVSNNDE"
     gene            2870..3343
                     /locus_tag="ACFM8S_001863"
     CDS             2870..3343
                     /locus_tag="ACFM8S_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX family type IV pilin"
                     /protein_id="HFC6314887.1"
                     /translation="MEQKGFTLIEMMIVVAILGIISVIAIPSYQSYIEKGYQSQLYTE
                     MVGINNISKQFILKNPLDDNQTIKSKLKIFVSGYKMNPKIAEKYNVSVHFVNKEKPRA
                     YSLVGVPKTGTGYTLSVWMNSVGDGYKCRDKASAEAYSETLSADAGCEAFSNRKK"
BASE COUNT          972 a          691 c          867 g          888 t
ORIGIN      
        1 aatttaatcc acgatacatt accagttaac gttctattgc ttatgtgtac acgaaaacaa
       61 caaggtttca cgctaacaga gctgctcatc gtgatggtca ttgcagccat tatggcgatg
      121 atagccctcc ccaatatgag ccaatggatt gcatcccgcc gcattgccag tcacgcggag
      181 cggattgcca accttttgcg tttctccagg ggcgaagccg tccggctcaa tctccctgtc
      241 tatatctgtc ctgttcaagt taaaaaagac ggtgcgccca acaatagatg tgacttcagc
      301 aagaaggggc ggggaatgtt ggctttcggc gacaaaaacg gcaataaggc atatgacggc
      361 gatgcggcgg atgttttcct ccgcagcgtg gtgttgaatg ataccgacga cagccggatt
      421 aattatgcct tcaaccatat cgctttcggt tcgtctcagc cgaccgccga ccgtgtagtt
      481 tggacattca atcaaaacgg gacgttcggt tatacgaaag accagcatct tacacgcaaa
      541 tccagctttt tttattccga cggttatatc caaatcgtgc tgacagatgc gagagcagtt
      601 tcagatgccg ataagaaatt ccgttcggcg gtggttttga ttgacagcag cggcagggtc
      661 gaagtttgcc ctaggggtga tcggcgtact cggcgtacta tgtgccagta taaataacag
      721 tttcagtttt aaaaatgaat atgaagaata atgattgctt ccgcctgaaa gattcccagt
      781 ccggtatggc gctgatagaa gtcttggtcg ctatgctcgt tctgaccatc ggtattttgg
      841 cactattgtc tgttcagttg cggacagtcg cttccgtcag ggaggcagag acgcaaacca
      901 tcgtcagtca aatcacgcaa aacctgatgg agggaatgtt gatgaatccg accattgatt
      961 cggacagcaa caagaaaaac tataatcttt acatgggaaa ccatcataca ctatcagctg
     1021 tggatggcga ttttgcggtt gatgccgtga aaagcaagaa ggatttggca aaagcccagt
     1081 tggacaggtt cggttatgaa ttgaaaaatg ccttgccgga tgcggcagcc atccattacg
     1141 ccatctgcaa ggattcgtcg ggtgctgcgc cgacattgtc cgccggcggt gctttttctc
     1201 aaaattgcga tggtagtgca aatggggata ctttgattaa agtattgtgg gtaaatgatt
     1261 cggcagggga ttcggatatc acccgtacga atcttgagac gaacggcgac aatatcgtat
     1321 atacctatca ggcaagggtc ggaggtcggg aatgagacgt aaaatgctaa acgtaccaaa
     1381 aggcaattat gatggtatga aaggttttac cattattgaa tttttggttg cgggcatgct
     1441 cagtatgatt gtcctgatgg cggtcggatc gagttacttc acatcccgga aattaaatga
     1501 tgcggcaaac gagcgtcttg ccgcgcaaca ggatttgcgg aatgcggcaa cattgattgt
     1561 ccgcgatgca agaatggcag ggggcttcgg ttgtttcaat atgtccgaac atcctgcaac
     1621 tgatgttgtt tccgatgtgg cgcaaaaaaa ccatcttttt tccttaaaaa ggaacagtac
     1681 aaataaactt atccccatag cggaatcttc aaatatcaga tatccgggtt ttacccagcg
     1741 ccttaacgca ttgattttcc aatacggaat cgatgatgtt aatgcaagcg ccgatactac
     1801 cgtcgtcagc agctgtgcca aaatagcaaa accgggtaag aaaatatcta ccttgcaaga
     1861 agcaaagagt gtattacaga ttactaatga tgataaacaa aatggaaata tcacccgtca
     1921 aaggcatgtg gtcaatgcct atgcggtcgg caggattgcc ggtgaggaag gtttgttccg
     1981 cttccaattg aatgatgatg ggcagtgggg caatcctcag ttgttggtta aaaagattaa
     2041 taaaatggat atacggtata tttatgtttc cggctgtcct gaagatgacg atgccggcaa
     2101 agaggaaaca ttcagatata cggataaatt caacagcgcc caagatgctg ttacgcctgc
     2161 cggggtggag gttttattga gtagcggtac tgataccaag attgccgctt cttcagacaa
     2221 tcatatttat gcttaccgta tcgatgcgac aatacgcggg ggaaatgtat gcgcaaacag
     2281 aacactttga cgggaatccc gacttctgac ggacagaggg ggtttgcact gtttatcgtg
     2341 ctgatggtga tgatcgtcgt ggcttttttg gttgtaactg ccgcgcagtc ttacaatacc
     2401 gagcagcgga tcagtgccaa cgaatcagac aggaaattgg ctttgtcttt ggccgaggcg
     2461 gctttgcggg aaggcgaact tcaggttttg gatttggaat atgatacgga cagtaaggtt
     2521 acatttagcg aaaactgtga aaaaggtctg tgtaccgcag tgaatgtgcg gacaaataat
     2581 actggtaatg aagaggcttt tgacaatatc gtggtgcaag gcaagcccac cgttgaggcg
     2641 gtgaagcgtt cttgcactgc aaaatctaca ggcctgtgca ttgacaagaa agggatggaa
     2701 tataagaaag gcaagggaag cgtcagcaaa tcaccacgct atattatcga atatttgggc
     2761 gtgaagaacg gagaaaatgt ttatcgggtt actgccaagg cttggggtaa gaatgccaat
     2821 accgtggtcg tccttcaatc ttatgtaagc aataatgatg agtaataaaa tggaacaaaa
     2881 agggtttaca ttgattgaga tgatgatagt cgtcgcgata ctcggcatca tcagcgtcat
     2941 tgccatacct tcttatcaaa gttatattga aaaaggctat cagtcccagc tttatacgga
     3001 gatggtcggt atcaacaata tttccaaaca gtttattttg aaaaatcccc tggacgataa
     3061 tcagaccatc aagagcaaac tgaaaatatt tgtctcaggc tataagatga atccgaaaat
     3121 tgccgaaaaa tataatgttt cggtgcattt tgtcaataag gaaaaaccaa gggcatacag
     3181 cttggtcggc gttccaaaga cggggacggg ttatactttg tcggtatgga tgaacagcgt
     3241 gggcgacgga tacaaatgcc gtgataaggc ttctgccgaa gcctattcgg agaccttgtc
     3301 cgcagatgcc ggctgtgaag ccttctctaa tcgtaaaaaa taaggttgtt ttgccaatac
     3361 cgtctgaaaa tcaatgttca gacggtattt ttatgggtat agtggattaa caaaaatc
//
LOCUS       DAXHSF010000178         3393 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.178, whole genome shotgun sequence.
ACCESSION   DAXHSF010000178 DAXHSF010000000
VERSION     DAXHSF010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.178"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            248..3358
                     /gene="pilC"
                     /locus_tag="ACFM8S_001864"
     CDS             248..3358
                     /gene="pilC"
                     /locus_tag="ACFM8S_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC family type IV pilus tip adhesin"
                     /protein_id="HFC6314888.1"
                     /translation="MNKTLKRRVFRHTALYAAILMFSHTGGGGAMAQTYKYAIIMNEG
                     NQLEVKGNGQYSTIKDKDRERKYTYHQGRGGGGSVSFNNSDELVSRQSGTAVFGTATY
                     LPPYGKVSGFDERKLKERTNALNWIHTTHPGLIGYSYADVVCRDSTGCPKLVYKTQFT
                     FGNSGLAKKANGGGLDIYVDKSRDNSPIYKLKDHPWLGVSFNLGSENTVKNSKSSNKL
                     ISSFSEDNNNQTIVSTTEGSPISLGDQQREHTAVVYYLNAKLHLLDKKQIQNITDKTV
                     QLGVLKPSIDVRQQKGVNWLSFWASWDIKDTGQIPVKLGLQQVKAGRCINKPNPNPNK
                     KDLSPALTAPALWFGPVKDGKAEMYSASVSTYPDSSSSRIFLQNLERKTDPGRPGRHS
                     LKPLSDTQIKSREPNFTGRQTVIRLDKGVHQIKLKGNEVEGFKGNNGNDTFGIVREGS
                     FTPDASEWKKVLLPWTVRDSNDDGRFNAVNKEENNGKPKYSQKYRSRDNNNRDLGDIV
                     NSPIVAVGEYLATSANDGMVHIFKKGNGDARNYSLKLSYIPGTMPRKDIQNTESTLAK
                     ELRTFAEKGYVGDRYGVDGGFVLRQVNNLNGQDRVFMFGAMGFGGRGAYALDLTKAEN
                     GNPTAVSLFDVKNDNNGKNSNNSNNSVQLGYTVGTPQIGKTHNGKYAAFLASGYATKD
                     INNGENKTALYVYDLESSGTLIAKIEVKDGKGGLSSPTLVDKDLDGTVDIAYAGDRGG
                     NMYRFDLSSDNPTKWSVRTIFKGTPDKPITSAPAISQLKDKRVVIFGTGSDLSEDDVD
                     NTDEQHIYGIFDDDTATTGSVNFSGTGGGLLEQHLTQENKTLFLTDYKRSDGSGSKGW
                     MVKLQPGQRVTVKPTVVLRTAFVTIHKYTGTDKCGAETAILGINTADGGKLTKKSARP
                     IVPAANSKVAQYSGDKKTSSGKSIPIGCMEKDNGIVCPNGYVYDKPVNVRYLDEKKTD
                     GFSTTADGDAGGSGIDPDGKRSGKNNRCFSQKGVRTLLMNDLDSLDITGPTCGMKRIS
                     WREIFY"
BASE COUNT          965 a          864 c          886 g          678 t
ORIGIN      
        1 caaccagcaa ttcggcgggc gcggaatcgg gcggtttacc gaaccccggc gttcgcggcg
       61 cgcctgccgc cgacggtatc ccgcgaagca agatttaagg gataaaatat gttccaacac
      121 acagggcggc acataaggcg ccgccctgat tcggaagggc ttgcacccct cccggacaaa
      181 gcctgatcct gccgccccga aggacggatg cccgaagggc ggggggtttg accgaaaagg
      241 aaatacgatg aataaaactt taaaaaggcg ggttttccgc cataccgcgc tttatgccgc
      301 catcttgatg ttttcccata ccggcggggg gggggcgatg gcgcaaacct ataaatacgc
      361 tattatcatg aacgagggaa accagctcga ggtaaagggg aatgggcaat attcaacaat
      421 aaaggacaaa gacagggaac gcaaatatac ttatcatcag ggcagaggag gaggaggctc
      481 tgtctcattc aacaatagcg atgagcttgt ttctcgacaa agcggtactg ccgtttttgg
      541 cacagccacc tacctgccgc cctacggcaa ggtttccggt tttgatgaaa gaaagctgaa
      601 agagcgcacc aatgccctta attggattca tacgacccac ccagggttga taggctacag
      661 ctacgccgat gtcgtatgca gagacagcac aggctgtccc aaacttgtct ataaaaccca
      721 atttaccttc ggtaattccg gtttggcaaa aaaggcaaac ggcggcgggc tggatatata
      781 cgtagacaaa agccgcgaca attcgcccat ttacaaattg aaagatcatc catggttggg
      841 cgtatctttc aatttgggca gcgagaatac cgtcaaaaat agcaaatcat ccaacaaatt
      901 gatatcttct tttagtgaag acaataataa tcaaaccatc gtctctacga cagaaggctc
      961 ccctatttcc cttggcgacc agcagcgcga acataccgcc gtggtctatt atctgaacgc
     1021 caagctgcac ctgctggata aaaaacagat tcaaaatatc accgacaaaa cagtgcagtt
     1081 gggtgtcttg aagccgagca tcgatgtgcg gcagcagaaa ggtgtcaact ggctaagttt
     1141 ttgggctagt tgggacatta aagataccgg gcagattcca gtcaagctcg gcctgcagca
     1201 agtcaaagca ggccgctgca tcaataaacc gaaccccaat cccaacaaaa aagacctttc
     1261 gccggccctg actgcccccg cgctgtggtt cggacctgtg aaagatggta aggcggagat
     1321 gtattccgct tcggtttcta cctaccccga cagttcgagc agccgcatct tccttcaaaa
     1381 tctggaaaga aaaaccgacc ccggcagacc cggccgccat tccctcaaac ccttgagtga
     1441 tacgcagatt aaaagtagag agccgaattt cacggggcgg caaacagtca tccgattgga
     1501 taaaggcgta catcagatca aacttaaagg caatgaggtc gaaggtttta agggaaacaa
     1561 cggcaacgac actttcggca ttgttagaga agggagcttc acgcctgatg ccagcgaatg
     1621 gaaaaaagtg ctgctgcctt ggacggttcg agattccaat gatgacggtc gatttaacgc
     1681 agtcaacaaa gaagaaaaca acggcaagcc aaaatacagt caaaaatacc gcagccgcga
     1741 caacaacaat cgcgatttgg gcgacatcgt caacagcccc atcgtggcgg tcggcgagta
     1801 tttggctact tccgccaacg acgggatggt gcatatcttc aaaaaaggca acggggacgc
     1861 gcgcaactat agtctgaagc tcagctacat ccccggcacg atgccgcgca aggatattca
     1921 aaacaccgaa tccacccttg ccaaagagct gcgcaccttt gccgaaaaag gctatgtggg
     1981 cgaccgctac ggcgtggacg gcggctttgt cttgcgccaa gttaacaact taaatgggca
     2041 agaccgcgtg tttatgttcg gcgcgatggg ctttggcggc agaggtgcat acgccttgga
     2101 tttgaccaaa gccgaaaacg gtaacccgac cgccgtttcc ctgtttgatg tcaaaaatga
     2161 caataatggc aagaatagca ataatagcaa taatagcgtg caattaggct acaccgtcgg
     2221 cacgccgcaa atcggcaaaa cccacaacgg caaatacgcc gccttcctcg cctccggtta
     2281 tgcgactaaa gacattaaca acggcgagaa taaaaccgcg ctgtatgtgt atgatttgga
     2341 aagcagcggt acgctgattg caaaaatcga agtgaaggac ggcaagggcg ggctttcgtc
     2401 ccccacgttg gtggataaag atttggacgg cacggtcgat atcgcctatg ccggcgatcg
     2461 cggcgggaat atgtaccgct ttgatttgag cagcgacaat ccgaccaaat ggtctgtacg
     2521 tactattttt aaaggcacgc cggataagcc gattacctcc gcgcccgcca tttcccaact
     2581 gaaagacaaa cgcgtggtta tcttcggtac gggcagtgat ttgagtgagg atgatgttga
     2641 taatacggat gaacaacata tttacggtat cttcgacgac gatacggcga cgacgggttc
     2701 tgtaaacttc agcggtacgg gaggcgggct gctcgagcaa caccttactc aggaaaataa
     2761 aaccttattc ctgaccgatt acaagcgatc cgacggatcg ggcagtaagg ggtggatggt
     2821 gaaattgcag cccggacaac gcgttaccgt caaaccgacc gtggtattgc gtaccgcctt
     2881 tgtaaccatc cataaatata cgggtacgga caaatgcggc gcggaaaccg ccattttggg
     2941 tatcaatacc gccgacggcg gcaagctgac caagaaaagc gcgcgcccga ttgtgccggc
     3001 agccaattcg aaggtcgcgc aatattccgg cgataagaaa acttccagcg gcaaatccat
     3061 ccctataggt tgtatggaaa aagacaatgg aatcgtctgc ccgaacggat atgtttacga
     3121 caaaccggtt aatgtgcgct acctggacga aaagaaaaca gacggatttt caacaacggc
     3181 agacggcgat gcgggcggca gcggtataga ccccgacggc aagcgttccg gcaaaaacaa
     3241 ccgctgcttc tcccaaaaag gggtgcgcac cctgctgatg aacgatttgg acagcttgga
     3301 cattaccggc ccgacgtgcg gtatgaaacg aatcagctgg cgtgaaatct tctactgatt
     3361 tgcacgcgaa aatgccgtcc gaaaggtttt cgg
//
LOCUS       DAXHSF010000179         3329 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.179, whole genome shotgun sequence.
ACCESSION   DAXHSF010000179 DAXHSF010000000
VERSION     DAXHSF010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.179"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(205..1056)
                     /locus_tag="ACFM8S_001865"
     CDS             complement(205..1056)
                     /locus_tag="ACFM8S_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HFC6314889.1"
                     /translation="MTLLGIKLKQTQQLNQRLQQSLRVLQMSGIELEREVENWLSDNP
                     LLERKDPDEFSDAGFSHYTAPARQIGGDEGEDMLSNIAGEEDFKQYLHAQVCEHPLPD
                     QEAACVHILIDFLDEQGYLTDSIEDILDHTPLEWMLDEAMLQHELTALKKFDPAGVAA
                     ADLNESLILQIERSGECAAKTAALHIVRNALDSIDGNRSQTLARIKKRLPQTDSGTLE
                     AALDLIASLNPFPAAGFASSTPTPYSDEALANLLAFRGMEVSRRTVAKYRESFEIPAA
                     HKRKTAE"
     gene            1399..2913
                     /gene="katA"
                     /locus_tag="ACFM8S_001866"
     CDS             1399..2913
                     /gene="katA"
                     /locus_tag="ACFM8S_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HFC6314890.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLAQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTHDITKYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPAGYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRSQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGEFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGIGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDAQKQALFGNTAAA
                     MGDAPDFIKYRHIRNCYRCDPAYGEGVAKALGLTVEDAQAARATDPALGQAGLL"
     gene            2989..3210
                     /locus_tag="ACFM8S_001867"
     CDS             2989..3210
                     /locus_tag="ACFM8S_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314891.1"
                     /translation="MPSETSFDRFRRHFPIPPPPFQWAFFIKRKISRHSRPTAHFSAV
                     IPAPSPPVQTGTKTFPRHSRESGNLERKI"
BASE COUNT          794 a          933 c          862 g          740 t
ORIGIN      
        1 aaacccccgt cattcccgtg aaagcgggaa tccaggttcg ttgagtttcg gtcatttccg
       61 ataaattctt gcagctttgc gtttctagat tccctctttc gcgggaatga cgggatgcag
      121 gtttccgcgc ggacggattc ggcattcccg ctttcgcaga aataccggct tttggtttgt
      181 tgtctttata agattattcg gcaattattc tgcggttttg cgtttgtgtg ctgccggaat
      241 ctcaaaggat tctctgtatt tggcaacggt gcggcgggaa acctccatgc cgcggaaagc
      301 cagcaggttg gcgagtgcct cgtcagaata cggcgtgggc gtggacgagg caaaaccggc
      361 ggcgggaaag ggattgagcg aagcaatgag gtcgagtgcg gcttcgagtg tgccgctgtc
      421 ggtttggggc aggcgttttt ttattcgtgc gagggtttgg ctgcggttgc cgtcaatgct
      481 gtcgagggcg tttcggacga tatgcagggc ggcggttttg gcggcgcatt cgcccgatct
      541 ttctatctgc agtatcagcg attcgttcaa atcggcggcg gccacgcctg ctgggtcgaa
      601 ttttttcaat gcggtcagct cgtgttgcag cattgcttca tccaacatcc actccaaggg
      661 cgtatggtcg aggatgtctt cgatgctgtc ggtcagataa ccttgctcgt caaggaaatc
      721 gataaggatg tggacgcagg cggcttcttg gtcgggaagg gggtgttcgc acacttgcgc
      781 gtgcaggtat tgcttgaaat cttcttcgcc ggcgatgttg gacagcatat cttcgccttc
      841 gtctccgccg atttggcgcg cgggcgcagt gtaatgggaa aacccggcat cggaaaattc
      901 atctgggtct ttgcgttcga gcagggggtt gtccgacagc cagttttcga cctcgcgttc
      961 aagttcgata cccgacatct gcaatacgcg caaagattgt tgcagccgct ggttgagctg
     1021 ctgggtctgt ttgagcttta ttccgagtaa cgtcatgata atgcgagaaa attattcttt
     1081 tggtctgtcg gcggcagatg atgccgggaa acatcattat aaatggtttt atgtagggat
     1141 tttgattcgt ttcgataaca aaaaacaaac gaaaatagaa tattgattgc ttataacaat
     1201 attaaatcga tttcatagtt ttaatagcga aaatcttagc gtatagtcgc atccatagtt
     1261 tttacaaaag gaaaacaaaa tgtcgattca agaaatttat tgcaatcaag aaaccggtta
     1321 cgaatacgct ttccgccaaa tcgtactgta agccgtttgt cgtatctttt gattaaccaa
     1381 ggaaaaggaa cacgagctat gactacctcc aaatgccccg taacccatct gaccatgaac
     1441 aacggcgcgc ctgttgccga caatcaaaac agcctgaccg ccggtccgcg tggccccctg
     1501 ctggcgcagg atttgtggct gaatgaaaaa ctcgccgact tcgtgcgcga agtcatcccc
     1561 gaacgccgta tgcacgccaa aggttcgggc gcgttcggta cgtttaccgt aacgcacgac
     1621 atcaccaaat acacccgcgc caaaatcttc agcgaagtcg gcaaaaaaac cgagatgttc
     1681 gcccgtttca ccaccgtggc aggcgaacgc ggcgcagccg atgcagaacg cgacatccgc
     1741 ggttttgcct tgaaatttta taccgaagaa ggcaactggg atgtggtcgg caacaacacg
     1801 cccgtgttct tcctgcgcga cccgcgtaag ttccccgacc tgaacaaagc cgtcaaacgc
     1861 gacccgcgca ccaatatgcg ctctgccaca aacaactggg acttctggac gctgctgcct
     1921 gaagcgttgc accaagttac catcgtgatg agcgaccgcg gcatccccgc cggctaccgc
     1981 catatgcacg gcttcggttc gcatacttac agcttctgga acgaagcagg cgaacgtttt
     2041 tgggtgaaat tccacttccg cagccagcaa ggcattaaaa acctgaccaa cgaagaagcc
     2101 gccaaaatca tcgccgacga ccgcgaaagc catcaacgcg acctgtacga agccatcgaa
     2161 cgcggcgagt ttccgaaatg gacgatgtac atccaagtca tgcctgaagc cgatgcggaa
     2221 aaagtgccgt atcatccgtt tgacttgacc aaagtttggc cgaaaaaaga ctatccgctg
     2281 attgaagtgg gcgaattcga gttgaaccgc aatcccgaaa acttcttcgc cgatgtggaa
     2341 caatccgcct tcgcaccgag caacctcgtt cccggtatcg gcgcgagccc agataaaatg
     2401 ttgcaagccc gtttgttcaa ttacgccgac gcgcaacgct accgcttggg cgtgaacttc
     2461 cgccaaatcc ccgtcaaccg tccgcgttgc cctgttcaca gcaaccagcg cgacggacaa
     2521 ggacgcgccg acggcaacta cggcagcctg ccgcactacg aacccaacag cttcggccaa
     2581 tggcagcagc agcccgactt cgccgaaccg cctttgaaaa tcaacggcga cgcggcgcac
     2641 tgggactacc gccaagacga tgacgactat ttcagccaac cgcgcgccct gttcaacctg
     2701 atgaacgacg cgcagaaaca ggcattgttc ggcaacaccg ccgccgcaat gggcgacgcg
     2761 cccgacttca tcaaataccg ccatatccgc aactgctacc gttgcgatcc ggcatacggc
     2821 gaaggcgtgg caaaagccct cggactgact gtcgaagacg cacaagccgc ccgcgcgacc
     2881 gaccccgcac tgggtcaggc cggtttgctg taagggggca ttatgtggat ggaaattgaa
     2941 gaaatcctgt cccgcgccgt ccgctaccgg aaataaccgg gcataaaaat gccgtctgaa
     3001 acgtcgtttg accgcttcag acggcatttc cccatcccgc ccccgccgtt tcagtgggcg
     3061 ttttttatta aacgcaaaat atcccgtcat tcccgcccca cagcccactt ttccgccgtc
     3121 attcccgcac catccccacc ggttcaaacc ggcacgaaaa cttttccgcg tcattcccgc
     3181 gaaagcggga atctagaacg taaaatctaa agaaaccgtt tttcccgata agtttccgca
     3241 ccgacaactc tggattcccg cctgcgcggg aatgacgaat ccatccgcac ggaaaccaaa
     3301 atcccgtcat tcccacgaaa gtgggaatc
//
LOCUS       DAXHSF010000180         3329 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.180, whole genome shotgun sequence.
ACCESSION   DAXHSF010000180 DAXHSF010000000
VERSION     DAXHSF010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.180"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(258..647)
                     /locus_tag="ACFM8S_001868"
     CDS             complement(258..647)
                     /locus_tag="ACFM8S_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFC6314892.1"
                     /translation="MSTHEKLLTADNPILRQRAKAMRQEMSGAEAKLWQHLRAGRLNG
                     YKFRRQQPMGNYIVDFMCVTPKLIVEADGGQHTEQAAYDHARTAYLNSLGFTVLRFWN
                     HEILQQTNDVLTEILLVLQELEKQAAR"
     gene            complement(1411..2544)
                     /gene="carA"
                     /locus_tag="ACFM8S_001869"
     CDS             complement(1411..2544)
                     /gene="carA"
                     /locus_tag="ACFM8S_001869"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFC6314893.1"
                     /translation="MSTPALLVLADGSVFHGTSIGYEGSTSGEVVFNTSMTGYQEILT
                     DPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYAAGLIIRDLPLLHSNFRASESLHDYL
                     VRNKTVAIADIDTRRLTTLLREKGAQGGAILTGADATIEKAQELIAAFGSMVGKDLAK
                     EVSCTETYEWTEGEWALGKGFVTPDEQPYHVVAYDFGVKTNILRMLASRGCRLTVVPA
                     QTSAQDVLALNPDGVFLSNGPGDPEPCTYAIEAVQKLMESGKPIFGICLGHQLISLAI
                     GAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAVDADTLPANARITHKSLFDNTL
                     QGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKRA"
BASE COUNT          725 a          853 c          905 g          846 t
ORIGIN      
        1 tggggagagg gcattctccg aatttcggca acctttccta acactctaac cgccaagaca
       61 caagccttgt ggcttgttgc cctctctcta actctctccc acgggagaga ggactgggcg
      121 gttgttgggg ctaaggtttc tgtaaactaa tcaggttatg caggctgctt tttgtttttc
      181 agacggcctt tataaatgct caattcagtc ggctttatcc tattattttc aataatccaa
      241 tcaaaatcag ccatctgcta tcgtgcagct tgcttttcca attcttgcaa cacgagcagg
      301 atttctgtca gcacatcgtt tgtctgctgt aaaatctcgt gattccaaaa ccgcagtacg
      361 gtaaagccca agctgttgag atatgccgtc cgcgcgtggt cgtatgcggc ttgttctgtg
      421 tgctgccctc cgtctgcttc gacaatcaac ttgggcgtta cgcacataaa atcaacaata
      481 tagtttccca tcggctgctg gcggcggaat ttatagccgt tcagacgacc tgcccgcagg
      541 tgctgccaca attttgcttc cgccccgctc atttcttggc gcatggcttt ggcgcgttgg
      601 cgcaagatgg ggttgtcggc ggttaaaagt ttctcgtgcg tgctcattct gttgtcgggt
      661 tactgtttgt gtaagcctta cagtttgttg ccctctctct gactctctcc catgggagag
      721 aggacgtggt ggcggttggg attaagggtt ctgcaaggaa aacaagtcgt tcaggccact
      781 ttttactttt cagacgacct gcaatcaacg gttgtaaatc caagttgctt ttcaaaccgc
      841 ctgtaaccgc aaaacttgct gcaacctacc ccctcccccg tgggggaggg ctggggagag
      901 ggcattctcc gaatcgaggc aatctttccc taattcccta actgcctcaa tacaagcctt
      961 tcggcttgtt gccctctctc taacgctttc ccacgggaga gaggacggtg cggctgtagt
     1021 gattaagggt tttgcaagga aaacaaggaa aacaagttgt acaggctgtt ttttattttt
     1081 caagcgacct gaaatcaacg cttgtaaatc caagtcactt ttcaaactgc ctgcaaccgc
     1141 aaaatctgct gcaacctacc ccctcccccg tgggggaggg ctggggagag ggcattttcc
     1201 aagttgcggc aatctttccc aaatccctta gtcttctaaa atacaagcct tacggttgtt
     1261 gccaaatgaa acttcttcca tacccaaaac ttgcgtgtaa aacgcaatgg ttcagtcaat
     1321 gtcggcaacg gtcagtacca gatggtcgag tgcgctaatc ttcatttccc gctttctttt
     1381 tcaggcggcg gcagtgccgt ctgaaagcca ttatgcccgt tttgccgctt tcatattgcc
     1441 aatgaatttg tcaaacaaat agccgacatc ttgcggaccg gggctggctt cggggtggcc
     1501 ttggaagcag aacacaggtt tgtcggtcag ctcgatgcct tgcaaagtgt tgtcaaacaa
     1561 ggatttgtgg gtaatgcgtg cgttagcggg cagggtgtcg gcatcaacgg caaaaccgtg
     1621 gttttggctg gtgatgacga ctttgccgct gtccaaatct tgcacagggt ggttcgcacc
     1681 gtggtggctg aagcgcattt tcagggtttt cgcgccgata gcgaggctga tgagctggtg
     1741 tcccaagcag atgccgaaaa tcggtttgcc gctttccatc agtttttgca cggcttcgat
     1801 ggcgtaggtg caaggctcgg ggtcgccggg gccgttggac aggaacacgc cgtcgggatt
     1861 gagtgccaac acgtcttgtg cgctcgtttg ggcggggacg acggtcaggc ggcagccgcg
     1921 cgaggcgagc atacgcagga tgttggtttt cacgccgaaa tcgtaggcga cgacgtggta
     1981 aggctgttcg tcaggggtaa cgaaaccctt gcccaatgcc cattcgcctt ccgtccattc
     2041 gtaagtttcc gtgcaggaaa cttcttttgc caagtctttg ccgaccatac tgccgaacgc
     2101 ggcgatgagt tcttgcgctt tttcgattgt ggcatccgca ccggtcagaa tcgcgccgcc
     2161 ttgcgcgcct ttttcgcgca acagcgtggt caggcggcgg gtatcgatgt cggcgatggc
     2221 gacggttttg ttgcgtacca gatagtcgtg caggctttcg gaggcgcgga agttgctgtg
     2281 caagagcggc aggtcgcgga taatcaggcc ggcggcataa acgctgcggc tttcttcatc
     2341 ttcggcgttg gtgccggtgt tgccgatgtg tgggtaggtg agggtaacga tttgtttgca
     2401 gtaggacggg tcggtcagga tttcctgata gccggtcatc gaagtattga acacgacttc
     2461 gccggaagtc gaaccttcgt aaccgattga tgtgccgtgg aatacgctgc cgtcagcgag
     2521 gacgaggagg gcgggggtgc tcatgatggg aatcctgttt ttaataaaat tcttgacaat
     2581 gccgtctgaa gggacttcgg cagggggcat ccggtcggaa aaatccggtc aaaaaaaaca
     2641 cgccgcgcaa gatagacgcg taacgtgctt ttcgggcaag tgcctatacc ctgaaagcag
     2701 catattttaa gacgaaacat acccgatttc aagcgcaaac gcggcaggcg ggctgatatg
     2761 ttcgcgcgaa aacggaaatc cgtggcgtaa aaccgcccgc tccgcgccca ctatgcccgt
     2821 tacgcatttc cccacgtccg tccggcgaaa ctatgggaat acccgaatcg tcaggaatac
     2881 ctgaatcgtc aggaatacct gaatcgtcag gaatacccga atcgtcagga atacccgaat
     2941 cgtcaggaat acccgaacca tcattcccgc gcaggcggga atccagacct gtcggcacag
     3001 aaacttatcg ggtataacgg tttcttgaga ttccgcattc tagattcccg cctgcgcggg
     3061 aatgacggcg cataagtttc tgtgttgatt tgagtctatg ggaacggtgt aggattggtg
     3121 gaatcgttgg aatcggtggg ctgaagccca ccctataact caccttttac gaatcctcct
     3181 acaccctata caacaccttg aaaatccacc ttgtcgggaa tgcccgaacc gtcagaaata
     3241 cccgaatcgt cattcccgcg caggcgggaa tccagacctg tcggtgcaga aacttatcgg
     3301 gcaaaacggt ttcttgagat tccgcatcc
//
LOCUS       DAXHSF010000181         3294 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.181, whole genome shotgun sequence.
ACCESSION   DAXHSF010000181 DAXHSF010000000
VERSION     DAXHSF010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.181"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            88..1317
                     /gene="sstT"
                     /locus_tag="ACFM8S_001870"
     CDS             88..1317
                     /gene="sstT"
                     /locus_tag="ACFM8S_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFC6314894.1"
                     /translation="MAFGKSLFHAIGRVSLVRQIAAGLALGIVIGSVSPQLGLAAGLF
                     GSLFVGALKAVAPVLVFILVAATIAQHQKGNKAHIRPIIVLYLIGTFSAALTAVIAGM
                     VFPTHIVLAGAGDVSAAPPSGIVEVLKSLLMNLVANPINAIANANYIGILAWALVLGA
                     ALRNHGSDVTRQVVADLAEAVSTVVKWIIRFAPLGIFGLVSSTIAETGFGALAGYAKL
                     LAVLLGCMAFIALVVNPAIVWWKIRRNPYPLVFTCLRESGVYAFFTRSSAANIPVNMA
                     LAKKLGLHEDTYSISIPLGATVNMGGAAITITVLAMAAAYTQGIQVDFATALLLSLVA
                     TVSACGASGVAGGSLLLIPLACSLFGISNDVAMQVVAVGFIIGVIQDSAETALNSSTD
                     VLFTAAADLGRQRNRAE"
     gene            1429..2922
                     /locus_tag="ACFM8S_001871"
     CDS             1429..2922
                     /locus_tag="ACFM8S_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC6314895.1"
                     /translation="MFKRSVIAMACIVALSACGGGGGGSPDVKSADTLSKPAAPVVTE
                     DVGEEVLPKEKKDEEAVSGAPQADTQDATAGKGGQDMAAVSAENTGNGGAATTDNPEN
                     KDEGPQNDMPQNAADTDSSTPNHTPAPNMPTRDMGNQAPDAGESAQPANQPDMANAAD
                     GMQGDDPSAGGENAGNTAAAESANQTGNNQPAGSSDSAPASNPAPANGGSDFGRVDLA
                     NGIKLDSGSENVTLTHCKDKVCDRDFLDEEAPPKSEFESLDDSGRINKYKKDGTSDKF
                     TNLVATEVKANGTNKYVIIYKDKSTSSARVRRSARSRRSLPAEMPLILVNQADTLIVD
                     GEAVILTGHSGNIFAPEGNYRYLTYGAEKLSGGSYALSVQGEPAKGEMLAGTAVYNGE
                     VLHFHTENGRSYPTKGRFAAKVDFGSKSVDGIIDSGDDLHMGTQKFKAVIDGNGFKGT
                     WTENGGGDVSGRFYGPAGEEVAGKYSHRPTDAEKGGFGVFAGKKEQD"
     gene            complement(2997..3212)
                     /locus_tag="ACFM8S_001872"
     CDS             complement(2997..3212)
                     /locus_tag="ACFM8S_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314896.1"
                     /translation="MFKLGVYACLGLFAGWVLLLIVQLWFSFLEAELFFKITLTMAGL
                     FVIILAALLVFGQYFSEKKMKDDGFIN"
     gene            complement(3205..>3294)
                     /locus_tag="ACFM8S_001873"
     CDS             complement(3205..>3294)
                     /locus_tag="ACFM8S_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFC6314897.1"
                     /translation="VVTMEKDLRRMLLFFKREAYVVILERDRV"
BASE COUNT          792 a          807 c          965 g          730 t
ORIGIN      
        1 acacagccga aaaataaaaa aacacagaga atgagttttc ttttaaaata caacattttt
       61 taacatatca caacataagg aagaattatg gcgttcggca aatccttatt tcatgcgatc
      121 ggccgcgtca gcctggtcag gcagattgcc gccggtttgg cgttgggcat cgtaatcggt
      181 tcggtttccc cgcaactggg cttggcggca ggcttgttcg gcagcctgtt tgtcggcgcg
      241 ctcaaagcgg tcgcgccggt tttggtattt attttggtgg cggccacaat cgcgcagcac
      301 caaaaaggca acaaggcgca tatcaggccg attatcgtcc tttacctcat cggtacgttt
      361 tccgcagccc tgaccgccgt catcgccggt atggttttcc cgacgcacat tgttttggcg
      421 ggcgcgggcg atgtgtccgc cgcgccgcct tccggcattg tggaagtatt gaaatcgctg
      481 ctgatgaacc tggtcgccaa cccgattaac gcgattgcca atgccaacta tatcggcatt
      541 ttggcttggg ctttggtttt gggcgcggcg ttgcggaatc acggttcgga cgttacgcgg
      601 caggtcgttg ccgatttggc ggaagcggtt tccaccgtcg tgaaatggat tatccgtttt
      661 gcccctttgg gcattttcgg gctggtgtcg tccacaatcg cggagacggg tttcggcgcg
      721 ctggcgggtt atgcgaagct gcttgcggta ctgttgggct gtatggcgtt tatcgcgctg
      781 gtggtcaatc ccgccatcgt gtggtggaaa atccgccgca acccttatcc gctggtgttt
      841 acctgcctgc gcgaaagcgg cgtgtatgcc ttctttaccc gttcttccgc cgccaatatc
      901 cccgtgaata tggctttggc gaaaaaactg gggctgcacg aagacactta ttcgatttcc
      961 atcccgttgg gggcgaccgt caatatgggc ggcgcggcga ttacgattac cgttttggct
     1021 atggcggcgg cgtacaccca aggcatacag gttgattttg ccaccgcgct gctgttgagc
     1081 ctggtggcga cggtcagcgc gtgcggcgcg tccggtgtgg cgggcggttc gctgctgctg
     1141 attccgctgg cgtgcagcct gttcggcatc agcaacgatg tcgctatgca ggtggtcgcc
     1201 gtcggcttta tcatcggcgt gattcaggat tcggcggaaa cggcgttgaa ttcttcaacc
     1261 gatgttttgt ttaccgccgc cgccgatttg ggcagacaga gaaaccgggc ggaataaacc
     1321 aagctatgcc gtctgaagcc cttttggcgt tcagacggca tatttttaca attttatggg
     1381 catatggcgg aaacaatacc ggctgccttg aatatgaagg agatgatgat gtttaaacgc
     1441 agtgtgattg caatggcttg tattgttgcc ctttcagcct gtgggggcgg cggtggcgga
     1501 tcgcccgatg ttaagtcggc ggacacgctg tcaaaacctg ccgcccctgt tgttactgaa
     1561 gatgtcgggg aagaggtgct gccgaaagaa aagaaagatg aggaggcggt gagtggtgcg
     1621 ccgcaagccg atacgcagga cgcaaccgcc ggaaaaggcg gtcaagatat ggcggcagtt
     1681 tcggcagaaa atacaggcaa tggcggtgcg gcaacaacgg ataatcccga aaataaagac
     1741 gagggaccgc aaaatgatat gccgcaaaat gccgccgata cagatagttc gacaccgaat
     1801 cacacccctg caccgaatat gccaaccaga gatatgggaa accaagcacc ggatgccggg
     1861 gaatcggcac aaccggcaaa ccaaccggat atggcaaatg cggcggacgg aatgcagggg
     1921 gacgatccgt cggcaggcgg ggaaaatgcc ggcaatacgg ctgccgccga atccgcaaat
     1981 caaacaggga acaaccaacc cgccggttct tcagattccg ctcccgcgtc aaaccctgca
     2041 cctgcgaatg gcggcagcga ttttggaagg gttgatttgg ctaatggcat caagcttgac
     2101 agcggttcgg aaaatgtaac gctaacccat tgtaaagaca aagtatgcga tagagatttc
     2161 ttagatgaag aagcaccgcc aaaatcagaa tttgaatcgt tagatgactc tgggcgaatt
     2221 aataaataca agaaagatgg aacaagcgat aaatttacta atttggttgc gacagaagtt
     2281 aaagctaatg gaactaacaa atatgtcatc atttataaag acaagtccac ttcatctgcg
     2341 cgagttaggc gttctgcacg gtcgaggagg tcgcttcccg ccgagatgcc gctaatcctc
     2401 gtcaatcagg cggatacgct gattgtcgat ggggaagcgg tcatcctgac ggggcattcc
     2461 ggcaatatct tcgcgcccga agggaattac cggtatctga cttacggggc agaaaaattg
     2521 tccggcggat cgtatgccct cagtgtgcaa ggcgaaccgg caaaaggcga aatgcttgct
     2581 ggcacggccg tgtacaacgg cgaagtgctg catttccata cggaaaacgg acgttcgtac
     2641 ccgaccaaag gcaggtttgc cgcaaaagtc gatttcggca gcaaatctgt ggacggcatt
     2701 atcgacagcg gcgatgattt gcatatgggt acgcaaaaat tcaaagccgt tatcgatgga
     2761 aacggcttta aggggacttg gacggaaaat ggcggcgggg atgtttccgg aaggttttac
     2821 ggcccggccg gcgaggaagt ggcggggaaa tacagccatc gcccgacaga tgcggaaaag
     2881 ggcggattcg gcgtgtttgc cggcaaaaaa gagcaggatt gatgttgatg ccgcccgaag
     2941 cgggggcgtt cagacggcat tgtgatgttt tgggtggggt ccggttcaag tccgcatcag
     3001 ttgataaacc cgtcgtcttt cattttcttt tcggaaaaat actggccgaa taccagcagg
     3061 gcggcgagga tgatgacaaa cagccccgcc atagttagtg tgattttgaa gaacaattcc
     3121 gcttcgagaa aagaaaacca gagttgcaca atcagcagca gcacccagcc ggcaaacagt
     3181 ccgagacagg cataaacgcc gagcttaaac acggtcccgc tccaaaatga cgacgtaggc
     3241 ctcgcgtttg aaaaacagca gcatacggcg caaatctttt tccatcgtta cgac
//
LOCUS       DAXHSF010000182         3289 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.182, whole genome shotgun sequence.
ACCESSION   DAXHSF010000182 DAXHSF010000000
VERSION     DAXHSF010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.182"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            101..232
                     /locus_tag="ACFM8S_001874"
     CDS             101..232
                     /locus_tag="ACFM8S_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314898.1"
                     /translation="MNKTWKRQVFRHTALYTAILMFSHTGGGGGRRKRKRKRINTLL"
     gene            202..3255
                     /gene="pilC"
                     /locus_tag="ACFM8S_001875"
                     /pseudo
     CDS             202..3255
                     /gene="pilC"
                     /locus_tag="ACFM8S_001875"
                     /inference="COORDINATES: protein motif:HMM:NF040838.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PilC family type IV pilus tip adhesin"
BASE COUNT          987 a          800 c          837 g          665 t
ORIGIN      
        1 caatcagcaa ttcggcgggc gcggaatcgg gcggtttacc gaacctcggc gttcgcggcg
       61 cgccgccccg tcccgtgaag gcaaactcaa ggaataaaag atgaataaaa cttggaaacg
      121 gcaggttttc cgccataccg cgctttatac cgccatattg atgttttccc ataccggcgg
      181 gggggggggc aggcgcaagc gcaaacgcaa acgcataaat acgctattgt aatgaacgcg
      241 caaaatctgc ctgaggtaaa gtgggggaat gactataaca aattggtgca aaaaatcaat
      301 gaacgcgaag ttacccatac gagttttttc agaatcaaaa aaagcgttag tttctcattc
      361 aataataaag atgaagttgt tgctgaaaaa aaagatgctg tcgttttcgg cgcggcgacc
      421 tacctgccgc cctacggcaa ggtttccggt tttgataccg ttaagctgac cgagcgcgcc
      481 aatgccctta attggattaa tacgacccac ccggggctga taggctatag ctaccaagat
      541 agcacatgct ccagcagtgg ttgtcctgaa gttgcctata gaacccaatt taccttcggc
      601 aattccgggt tggcaaaaaa gaaaacagac aacaagctgg atatatacga agacaaaagc
      661 cgcgacaatt cgcccattta taaagtgaag gattatcctt ggttgggcgt gtctttcaat
      721 ttgggcggag agagctcttt caaaccaaag agaaacggtt ctttggtatc ttcttttagc
      781 gaggacgtga cgcagcaaaa tggtgcgggc agccaacaca aagacaaaaa cctcgtttat
      841 acgacagacg attacaagag tcagaataat aaaaaccatc aggacaaaca ccacgccgtc
      901 gccttttatc tgaacgccaa gctgcacctg ctggataaaa aacacattaa aaatatcgtg
      961 caaggtaaaa cagttaattt gggtatcttg aaaacacgca tcgagccgac ggaagcatgg
     1021 aaaagacgga atagtaactt ttttaacggt agttggacgt atgaagagaa aggaacagtc
     1081 agcgtcaaac tcaaattgcc ggaagtcaaa gcaggccgct gcatcaacaa acagaacccc
     1141 aataaaaaca gcaaagcccc ttcccccgca ctgactgccc ccgcgctgtg gttcggacct
     1201 gtgaaaaatg gtaaggtgca gatgtattcc gcttcggttt ccacctaccc cgacagttcg
     1261 agcagccgca tcttccttca aaatctgaaa agaaaaaccg accccaacag acccggccgc
     1321 tattccctcg aaaccttgac tgagaacgat attaaaagtc gacagccgaa tttcacaggg
     1381 cggcaaaccg tcatccgatt ggatggcggc gtacatgaga tcaaacttaa aggcaatgag
     1441 gtcgaaggtt ttaagggaaa caacggcaac gacactttcg gcattgttaa ggacttgggc
     1501 gtcgatcctg ataccaatga gtggaaaaaa gtgctgctgc cttggacggt tcggggtttt
     1561 gctgatgaca gtaaatttaa agcattcaac aaagaaaaaa acaacgacaa caagccaaaa
     1621 tacagtcaaa aataccgcag ccgcgacagc agcaagcacg agcgcgattt gggcgacatc
     1681 gtcaacagcc ccatcgtggc ggtcggcgag tatttggcta cttccgccaa cgacgggatg
     1741 gtgcatatct tcaaaaaagg caacggcggg gacgagcgca actatagtct gaagctcagt
     1801 tatatcccgg gtacgatgcc gcgcaaggat attgaaaaca aagattccaa cctcgccaaa
     1861 gagctgcgcg cctttgccga aaaaggctat gtgggcgacc gctacggcgt ggacggcggc
     1921 tttgtcttgc gccgcattac agatgaccag gacaagcaaa aacatttctt tatgttcggc
     1981 gcaatgggcc ttggcggcag aggcgcatac gccttggatt tgaccaaagc cgacgacaat
     2041 gacccgacaa aagcctcttt gtttgatgta aaagataacg gcaataatgg caataatcgc
     2101 gtggaattag gctacaccgt cggcacgccg caaatcggca aaacccacaa cggcaaatac
     2161 gccgccttcc tcgcctccgg ttatgcgact aaaaccattg acagcacaga caataaaacc
     2221 gcgctgtatg tgtatgattt ggaaaacaac ggtacgctga ttagaaaaat cgaagtgaag
     2281 gacggcaagg gcgggctttc gtcccccacg ctggtggata aagatttgga cggcacggtc
     2341 gatatcgcct atgccggcga tcgcggcggg aatatgtacc gctttgattt gagcagcgac
     2401 aatccgtcca gctggactgt acgcactatt ttccaaggca cgaagccgat tacttccgcg
     2461 cccgccattt cccaactgaa agacaaacgc gtggtcatct tcggcacggg cagtgatttg
     2521 agtgaggaag atgttgataa taatgatata caatctattt acggtatttt tgacaatgac
     2581 acagacacgg gttttgcgca agacgggctg ggcaaagggc tgctcgagca aaagctggaa
     2641 aaagacaaag acggcaaaac gctgttttta agcgactaca aaaggtctga cggctcgggc
     2701 gacaagggct gggtagtgaa attgaaggaa ggacagcgcg ttaccgtcaa accgaccgtg
     2761 gtattgcgta ccgcctttgt aaccatccat aaatatacgg gtacggacaa atgcggcgcg
     2821 gaaaccgcca ttttgggcat caataccgcc gacggcggca agctgaccaa gaaaagcgcg
     2881 cgcccgattg tgccggaagc caatcaggct gtcgcgcaat attccggcca taagcaaacc
     2941 gccaaaggca aatccatccc tataggttgt atggaaaaag acaatggaat cgtctgcccg
     3001 aacggatatg tttacgacaa accggttaat gtgcgctacc tggacgaaaa gaaaacagac
     3061 gatttccccg tcacggcaga cggtgatgca ggcggcagcg gaacattcaa agagggtaaa
     3121 aaacccgccc gcaataaccg gtgcttctcc agaaaaggtg tgcgcaccct gctgatgaac
     3181 gatttggaca gcttggacat taccggcccg acgtgcggta tgaaacgaat cagctggcgt
     3241 gaagtcttct actgatttgc acgcgaaaat gccgtccgaa aggttttcg
//
LOCUS       DAXHSF010000183         3280 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.183, whole genome shotgun sequence.
ACCESSION   DAXHSF010000183 DAXHSF010000000
VERSION     DAXHSF010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.183"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..2661
                     /locus_tag="ACFM8S_001876"
     rRNA            <1..2661
                     /locus_tag="ACFM8S_001876"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            2758..2871
                     /gene="rrf"
                     /locus_tag="ACFM8S_001877"
     rRNA            2758..2871
                     /gene="rrf"
                     /locus_tag="ACFM8S_001877"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            2907..>3280
                     /locus_tag="ACFM8S_001878"
     CDS             2907..>3280
                     /locus_tag="ACFM8S_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFC6314899.1"
                     /translation="MLCPEKMSGMAGRHPYGVRSGLRRNGLKLWDIHFRMTRFIVARC
                     GLLFATLKGKTMKKMFVLFCMLFSCAFSLAAVNINAASQQELEALPGIGPAKAKAIAE
                     YRAQNGAFKSVDDLTKVKGIGPA"
BASE COUNT          868 a          717 c          977 g          718 t
ORIGIN      
        1 gattccgcaa gtagtggcga gcgaacgcgg aggagcctgt acgtaataac tgtcgagata
       61 gaagaacaag ctgggaagct tgaccatagt gggtgacagt cccgtattcg aaatctcaac
      121 agcggtacta agcgtacgaa aagtagggcg ggacacgtga aatcctgtct gaatatgggg
      181 ggaccatcct ccaaggctaa atactcatca tcgaccgata gtgaaccagt accgtgaggg
      241 aaaggcgaaa agaaccccgg gaggggagtg aaacagaacc tgaaacctga tgcatacaaa
      301 cagtgggagc gccctagagg tgtgactgcg taccttttgt ataatgggtc aacgacttac
      361 attcagtagc gagcttaacc gaatagggga ggcgtaggga aaccgagtct taatagggcg
      421 aatagttgct gggtgtagac ccgaaaccga gtgatctatc catggccagg ttgaaggtgc
      481 cgtaacaggt actggaggac cgaacccacg catgttgcaa aatgcgggga tgagctgtgg
      541 ataggggtga aaggctaaac aaactcggag atagctggtt ctccccgaaa actatttagg
      601 tagtgcctcg agcaagacac tgatgggggt aaagcactgt tatggctagg gggttattgc
      661 aacttaccaa cccatggcaa actaagaata ccatcaagtg gttcctcggg agacagacag
      721 cgggtgctaa cgtccgttgt caagagggaa acaacccaga ccgccagcta aggtccccaa
      781 tgatagatta agtggtaaac gaagtgggaa ggcccagaca gccaggatgt tggcttagaa
      841 gcagccatca tttaaagaaa gcgtaatagc tcactggtcg agtcgtcctg cgcggaagat
      901 gtaacggggc tcaaatctat aaccgaagct gcggatgcca gtttactggc atggtagggg
      961 agcgttctgt aggccgatga aggtgcattg taaagtgtgc tggaggtatc agaagtgcga
     1021 atgttgacat gagtagcgat aaagcgggtg aaaagcccgc tcgccgaaag cccaaggttt
     1081 cctgcgcaac gttcatcggc gtagggtgag tcggccccta aggcgaggca gaaatgcgta
     1141 gtcgatggga aacaggttaa tattcctgta cttgattcaa atgcgatgtg gggacggaga
     1201 aggttaggtt ggcaagctgt tggaatagct tgtttaagcc ggtaggtgga agacttaggc
     1261 aaatccgggt cttcttaaca ccgagaagtg acgacgagtg tctacggaca cgaagcaacc
     1321 gataccacgc ttccaggaaa agccactaag cttcagtttg aatcgaaccg taccgcaaac
     1381 cgacacaggt gggcaggatg agaattctaa ggcgcttgag agaactcagg agaaggaact
     1441 cggcaaattg ataccgtaac ttcgggagaa ggtatgccct ctaaggttaa ggacttgctc
     1501 cgtaagccct ggagggtcgc agagaatagg tggctgcgac tgtttattaa aaacacagca
     1561 ctctgctaac acgaaagtgg acgtataggg tgtgacgcct gcccggtgct ggaaggttaa
     1621 ttgaagatgt gcaagcatcg gatcgaagcc ccagtaaacg gcggccgtaa ctataacggt
     1681 cctaaggtag cgaaattcct tgtcgggtaa gttccgaccc gcacgaatgg cgtaacgatg
     1741 gccacactgt ctcctcctga gactcagcga agttgaagtg gttgtgaaga tgcaatctac
     1801 ccgctgctag acggaaagac cccgtgaacc tttactgtag ctttgcattg gactttgaag
     1861 tcacttgtgt aggataggtg ggaggcttag aagcagagac gccagtctct gtggagccgt
     1921 ccttgaaata ccaccctggt gtctttgagg ttctaaccca gacccgtcat ccgggtcggg
     1981 gaccgtgcat ggtaggcagt ttgactgggg cggtctcctc ccaaagcgta acggaggagt
     2041 tcgaaggtta cctaagtccg gtcggaaatc ggactgatag tgcaatggca aaaggtagct
     2101 taactgcgag accgacaagt cgagcaggtg cgaaagcagg acatagtgat ccggtggttc
     2161 tgtatggaag ggccatcgct caacggataa aaggtactcc ggggataaca ggctgattcc
     2221 gcccaagagt tcatatcgac ggcggagttt ggcacctcga tgtcggctca tcacatcctg
     2281 gggctgtagt cggtcccaag ggtatggctg ttcgccattt aaagtggtac gtgagctggg
     2341 tttaaaacgt cgtgagacag tttggtccct atctgcagtg ggcgttggaa gtttgacggg
     2401 ggctgctcct agtacgagag gaccggagtg gacgaacctc tggtgtaccg gttgtaacgc
     2461 cagttgcata gccgggtagc taagttcgga agagataagc gctgaaagca tctaagcgcg
     2521 aaactcgcct gaagatgaga cttcccttgc ggtttaaccg cactaaagag tcgttcgaga
     2581 ccaggacgtt gataggtggg gtgtggaagc gcggtaacgc gtgaagctaa cccatactaa
     2641 ttgctcgtga ggcttgactc tatcatttga agaacttcaa gagataaaag cttactgact
     2701 gattcagtca ttaccgaata tattgattaa ggctttaccg atttgtaaca gtttaagttt
     2761 ggcggccata gcgagttggt cccacgcctt cccatcccga acaggaccgt gaaacgactc
     2821 agcgccgatg atagtgtggt tcttccatgc gaaagtaggt cactgccaaa caccctttca
     2881 gaaaaccccc gattattcgg gggtttttgc tttgcccgga aaaaatgtcg gggatggcgg
     2941 gacggcatcc gtacggcgtc cggtcgggtt tgcggaggaa cggcttgaaa ctttgggata
     3001 ttcattttag aatgactcgt tttatcgtcg caagatgcgg tttattgttt gcaaccctta
     3061 aaggaaaaac catgaagaaa atgttcgtgc tgttctgtat gctgttctcc tgcgccttct
     3121 cccttgcggc ggtaaacatc aatgcggctt cgcagcagga gttggaggcg ctgccgggca
     3181 taggccctgc gaaggcgaag gccattgcgg aataccgtgc gcaaaacggt gcgttcaagt
     3241 ctgtagacga tttgaccaag gtaaagggca tcggccctgc
//
LOCUS       DAXHSF010000184         3231 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.184, whole genome shotgun sequence.
ACCESSION   DAXHSF010000184 DAXHSF010000000
VERSION     DAXHSF010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.184"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(118..1218)
                     /locus_tag="ACFM8S_001879"
     CDS             complement(118..1218)
                     /locus_tag="ACFM8S_001879"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFC6314900.1"
                     /translation="METQLYIGIMSGTSMDGADAVLVRMDGGKWLGAEGHAFTPYPDR
                     LRRQLLDLQDTGADELHRSRILSQELSRLYAQTAAELLCSQNLAPSDITALGCHGQTV
                     RHAPEHGYSIQLADLPLLAERTRIFTVGDFRSRDLAAGGQGAPLVPAFHEALFRDNRE
                     TRAVLNIGGIANISVLPPDAPAFGFDTGPGNMLMDAWTQAHWQLPYDKNGEKAAQGNI
                     LPQLLDRLLAHPYFARPHPKSTGRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVF
                     DAVSHAAADARQMYICGGGIRNPVLMADLADCFGTRVSLHSTAELKLDPQWVEAAAFA
                     WLAACWINRIPGSPHKATGASKPCILGAGYYY"
     gene            1543..3117
                     /locus_tag="ACFM8S_001880"
     CDS             1543..3117
                     /locus_tag="ACFM8S_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HFC6314901.1"
                     /translation="MAQIQMSANVKTINAVFAAMLVGTVGYFIYWGLGYTHYNYAALF
                     IIATMFGVFMAFNIGGNDVANSFGTSVGAGTLTIPQALLIAAVFEVSGAVIAGGEVTN
                     TIRKGIVDLKGVDFEPIQFVFIMMSALLAAALWLLFASKKGLPVSTTHSIIGGIVGSA
                     VCMAVMNDAASGDLIRWGKLGGIGVSWVLSPVLGGAVSYFLFSRVKKNVLDYNAWAEG
                     TLKGIKQEKKAYKERHRLFFEGLSEAEKVEYATKMAHDAQIYDEPEFDPQELQSEYYR
                     GLYAFDNRKNNVDSYKALHSWIPFIASFGAMMISAMLIFKGLKNLHLGMSNVNSFLTI
                     FMIGAAVWMGTFVFAKSLKRKDLGKSTFQMFSWMQVFTACGFAFSHGANDIANAIGPF
                     AAIMDVLRTNSVAAQNVVPPIAMLTFGIALIVGLWFVGKEVIKTVGTSLAEMHPASGF
                     TAELSAASVVMGASLMGLPVSSTHILVGAVLGIGLVNRNANWKLMKPIGLAWVITLPA
                     AAVLSVVCYLVLQAVF"
BASE COUNT          670 a          808 c          943 g          810 t
ORIGIN      
        1 gcagggtttg ttttgttttg aacgagtttg agtgtaaatg gattttaagg gtttgtaaac
       61 gaaagaggca aaaatgccgt ctgagcggcg gaaatggctt tcagacggta tttgcgctca
      121 ataataatat cccgcgccca gaatacacgg tttggatgcg ccggttgctt tgtgcggact
      181 accgggaatg cgattaatcc aacacgccgc caaccacgca aatgcggctg cttccaccca
      241 ttgcggatcg agcttcagtt cggcggtgct gtgcagggaa acgcgtgtgc cgaaacagtc
      301 tgccaaatcc gccattaaaa caggattgcg gatgccgccg ccgcaaatgt acatttgacg
      361 ggcatctgcc gctgcgtgtg agacggcgtc gaaaacggtt tgcgcggtaa aacgggaaag
      421 cgtccgtaat acgtcgtatc ggttttcgcc gccgtcaagg taggtttcga gccaatttag
      481 ggcaaacagt tcgcgccccg tgcttttggg gtggggtcgt gcgaaatacg ggtgggcgag
      541 cagcctgtcg agcagttgcg gcaatatgtt gccttgtgcc gctttttcac cgtttttgtc
      601 gtaaggcaac tgccagtgtg cctgcgtcca cgcgtccatc agcatattgc ccggccctgt
      661 gtcgaagccg aaggcgggtg cgtcgggggg gagtacgctg atgttggcaa tcccgccgat
      721 gttcagtacc gcgcgtgttt ccctgttgtc gcggaacagg gcttcgtgaa aggcggggac
      781 gagtggcgcg ccttgtccgc cggcagcaag gtcgcggctg cggaagtcgc cgacggtaaa
      841 aatccgcgtc cgttccgcca gcagcggcaa atcggcaagc tgtatgctgt aaccgtgttc
      901 cggcgcgtgt cggacggttt gcccgtggca gccgagggcg gtaatgtcgg acggtgcgag
      961 gttttgactg cacagcagtt cggcggcggt ttgcgcgtac aggcggctga gttcttgcga
     1021 caaaatcctg ctgcggtgca gttcgtctgc gcctgtgtcc tgcaaatcca gcaattggcg
     1081 gcgcagcctg tcgggatagg gggtaaaggc gtgcccttcc gcgcccagcc atttgccgcc
     1141 gtccatccgt accagcacgg catccgcccc atccatactg gttcccgaca tgatgccgat
     1201 gtaaagctgt gtttccatca tcactcccaa actggtgcaa aacgccattt taacgtgtat
     1261 tgacgctcgt ataccgattt gccgccgcag tgtaaataaa gtgtaaataa atgtttcaag
     1321 acggatggaa aaatattata atgcgcccgc aacatccagt agtagaagtg tcatacaaac
     1381 cgtttccggc agcagttttg cattcggtca ggtttggggg tattcggatg cggttcagaa
     1441 ggaaggatgc gtctgccata tcccgaaacg gcagttcgac cggaggcagc agtacagtgt
     1501 cggcaacact catgatttcc accacattaa aggaagattg ccatggctca aatccaaatg
     1561 agcgcaaatg ttaaaaccat caacgccgtc tttgccgcca tgctggtagg tacagtcggc
     1621 tattttattt attggggctt gggttatacc cattacaatt acgccgcctt attcattatt
     1681 gccacgatgt tcggcgtgtt tatggcgttc aacatcggcg gcaacgatgt tgccaattct
     1741 ttcggcacca gcgtcggtgc gggtacgctg accatcccgc aggctttgct gattgcggcg
     1801 gtatttgagg tcagcggcgc ggtcatcgcg ggcggcgagg taaccaatac catacgcaaa
     1861 ggcatcgtcg atttgaaggg tgttgatttc gaacccatac agtttgtgtt tattatgatg
     1921 tccgcgcttt tggcggcggc gttgtggctg ttgtttgcct cgaaaaaagg gcttccggta
     1981 tctaccaccc attccattat cggcggcatt gtcggcagcg cggtatgtat ggcggtaatg
     2041 aacgatgccg catcgggcga tttgatacgt tggggcaagc tgggcggtat tggtgtttct
     2101 tgggtattgt cgcccgtgtt gggcggcgcg gtgtcctatt tcctgttttc gcgcgtcaag
     2161 aaaaacgtct tagattacaa cgcttgggcg gaaggcacgc tcaagggcat caagcaggaa
     2221 aaaaaggcct ataaagaacg gcaccgcctg tttttcgagg gtttgtccga agccgaaaaa
     2281 gtcgagtacg ccaccaaaat ggcgcacgac gcgcaaattt acgacgaacc cgaattcgat
     2341 ccgcaagagc tgcaatcgga gtattaccgc ggtctttatg cgttcgacaa ccgtaaaaac
     2401 aatgtcgatt cctacaaagc actgcattct tggattccct ttatcgcttc gttcggcgcg
     2461 atgatgattt ccgctatgct gattttcaag ggcttgaaaa acctgcattt ggggatgagc
     2521 aacgtcaaca gcttcctgac catctttatg ataggcgcgg cggtgtggat ggggacgttt
     2581 gtgtttgcca aaagcctcaa gcgtaaagac ttgggcaaat cgacctttca gatgttttca
     2641 tggatgcagg tctttaccgc ctgcggcttc gcattcagcc acggggcgaa cgatatcgcc
     2701 aacgccatcg gtccgtttgc cgcgattatg gatgttttgc gtaccaacag cgttgccgcg
     2761 caaaatgtcg tccccccgat tgcgatgctg actttcggca tcgcgctgat tgtcggtttg
     2821 tggtttgtcg gtaaagaggt gattaaaacc gtcggtacga gtttggcgga aatgcacccc
     2881 gcctcgggtt ttaccgccga actgtccgcc gcatccgtcg tgatgggcgc gtcgctgatg
     2941 gggctgcccg tgtccagtac gcatatcttg gtcggcgcgg tactcggcat cggtctggtc
     3001 aaccgcaatg ccaactggaa actgatgaag cccatcggtt tggcgtgggt cattaccctg
     3061 cctgccgccg ccgtattgtc ggttgtctgc tacttggttt tacaggcagt attctgattg
     3121 taaaatactg atgccgtctg aacccgtgtt cagacggcat tttgttgatg gaatgtgcgg
     3181 gcttgtgcct tatgcacaat ctgttctgtc gggatatgcc gtttggtata g
//
LOCUS       DAXHSF010000185         3193 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.185, whole genome shotgun sequence.
ACCESSION   DAXHSF010000185 DAXHSF010000000
VERSION     DAXHSF010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.185"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            89..2347
                     /locus_tag="ACFM8S_001881"
     CDS             89..2347
                     /locus_tag="ACFM8S_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFC6314902.1"
                     /translation="MYTRIMEISPWTLRSAKLEKEHKRLQESLTSLGNGYMGMRGSFE
                     ETYSADSHLGTYIAGVWFPDKTRVGWWKNGYPKYFGKAINALNFSKVKIFVDGQEVDL
                     AKNDVAGFSVELDMQHGVLRRSFTVFGVRFNVCKFLSVAQKELAVIRWEAVSVDGKTH
                     QVRIDSIIDADVKNEDSNYEEKFWQVLDKGVSDSLSYIAAQTVANPFGVEQFIVNAEQ
                     TFAGSFKALSGSQTDWQVSNSFEVEVGGTPKTFEKRVIVTTSRDYQGLEAVKAAGRAL
                     SEKVAGVAFETLLDAHKAGWLHRWEIADVVIEGNDEAQQGIRFNLFQLFSTYYGEDAR
                     LNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAEPEVTRNLLQYRRNQLPQAQHNARE
                     QGLAGALYPMVTFTGIECHNEWEITFEEIHRNGAIPYAIYNYTNYTGDEGYLAKEGLE
                     VLVEVSRFWADRVHFSKRNGKYMIHGVTGPNEYENNINNNWYTNTLAAWVLDYTREAL
                     AKYPRPDLNVRAAELEKWADISANMYRPYDEELGVFVQHDGFLDKDIRPVSALSPDDL
                     PLNQKWSWDKILRSPFIKQADVLQGIYFFGDRFNIDEKRRNFDFYEPMTVHESSLSPC
                     IHSILAAELGKEEKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLAIVQGFAQMK
                     TWGGKLSFAPFLPGAWTGYAFHINYRGRLIKVAVGKENVVFTLLKGEPLDLQVYGKDI
                     TLDGSHAAALEK"
     gene            2360..3025
                     /gene="pgmB"
                     /locus_tag="ACFM8S_001882"
     CDS             2360..3025
                     /gene="pgmB"
                     /locus_tag="ACFM8S_001882"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFC6314903.1"
                     /translation="MTFTAVLFDLDGVITDTAEYHYRAWKKLAEELGIGIDRKFNEQL
                     KGVSRDDSLKRILAHGGKTVGEAEFAELTRHKNDNYVEMIQAVKPEDVYPGILPLLEA
                     LKANGKKIALASASKNGPFLLERMGLTHFFDAIADPAAVAHSKPAPDIFLAAAEGVGA
                     DIRRCIGIEDAAAGVAAIKAAGALPIGVGKAEDLGSDIALVSGTAELTYAYLQNVWAQ
                     SGR"
BASE COUNT          810 a          969 c          804 g          610 t
ORIGIN      
        1 tgcggcttcg tcgccttgtc ctgatttttg ttaatccact ataagatttc accattccct
       61 caaatcaatc caaacaggag cttcataaat gtacacaaga attatggaaa tcagcccttg
      121 gacgctgcgt tcggcaaagc tggaaaaaga acacaaacgg ctgcaagaga gcctgaccag
      181 tttgggcaac ggctatatgg gtatgcgcgg cagctttgag gaaacctatt ccgccgacag
      241 ccacttgggt acctatatcg ccggtgtgtg gtttcccgac aaaacccgcg tcggctggtg
      301 gaaaaacggc taccccaaat atttcggtaa agccatcaac gcgctcaatt tcagcaaagt
      361 caaaatcttt gtcgacggac aagaggtgga cttggcgaaa aacgatgttg ccggcttctc
      421 cgtcgaactc gatatgcagc acggcgtgtt gcgccgctcg ttcaccgtat tcggtgtgcg
      481 tttcaatgtg tgcaaattcc tgtccgtcgc gcaaaaagag ctggcggtta tccgctggga
      541 agccgtatcc gttgacggca aaacccacca agtccgcatc gattccatca tcgatgccga
      601 cgtgaaaaac gaagactcca actacgaaga aaaattctgg caggtattgg acaaaggcgt
      661 ttcagacagt ctctcctaca ttgccgccca aaccgttgcc aaccctttcg gcgtggaaca
      721 atttatcgtc aacgccgagc aaactttcgc cggcagcttc aaagccctca gcggcagcca
      781 aaccgactgg caagtctcca attctttcga ggtcgaagtc ggcggcacgc ccaaaacctt
      841 tgaaaaacgg gttattgtta ccaccagccg cgattatcag ggcttggaag cagtgaaagc
      901 cgcaggccgc gccttgtcgg aaaaagtcgc aggcgttgcg tttgaaacct tgctggacgc
      961 gcacaaagca ggctggctgc accgttggga aatcgccgac gtggtcatcg aaggcaacga
     1021 cgaagcgcag caaggcatcc gtttcaatct gttccaactg ttctccacct actacggcga
     1081 agacgcgcgt ctgaacatcg gcccgaaagg ctttaccgga gaaaaatacg gcggcgcgac
     1141 ctattgggac accgaagcct acgccgtgcc gctctacctc gcactggcgg aacccgaagt
     1201 tacccgcaac ctgctgcaat accgccgcaa ccaactgcca caggcgcaac acaacgcgcg
     1261 cgaacagggc ttggcgggcg cactctatcc gatggtaacg tttacgggca tcgagtgcca
     1321 caacgaatgg gaaatcacct tcgaggaaat ccaccgcaac ggcgcgattc cttacgccat
     1381 ctacaactac accaactaca ccggcgacga gggctatctt gccaaagaag gcttggaagt
     1441 cttggtcgaa gtgtcccgct tctgggcgga ccgcgtccac ttctccaaac gcaacggcaa
     1501 atacatgatt cacggcgtaa ccggcccgaa cgaatacgaa aacaacatca acaacaactg
     1561 gtacaccaac accctcgccg catgggtatt ggactacacc cgcgaagccc tggcgaaata
     1621 cccgcgtccg gatttaaacg tgcgtgccgc cgagttggaa aaatgggcgg acatcagcgc
     1681 gaatatgtac cgtccgtatg acgaagaact cggcgtattc gtacaacacg acggattcct
     1741 cgacaaagac atccgccccg tgtccgcgct ttcgcccgac gatttgccgc tcaaccagaa
     1801 atggtcgtgg gacaaaatcc tgcgttcgcc ctttatcaaa caggcggacg tattgcaagg
     1861 catctatttc ttcggcgacc gtttcaatat cgacgaaaaa cgccgcaact tcgacttcta
     1921 cgaaccgatg accgtgcatg aaagctcgtt gtcgccgtgt atccactcta ttctcgccgc
     1981 cgaactgggc aaagaagaaa aagccgtgga aatgtaccag cgcaccgccc gcctggactt
     2041 ggacaactac aacaacgaca ccgaagacgg cctgcacatc acctccatga ccggctcgtg
     2101 gctcgccatc gtccaaggtt tcgcccaaat gaaaacctgg ggcggcaaac tcagctttgc
     2161 accgttcctg ccgggcgcat ggacaggcta cgccttccat atcaactacc gaggccgtct
     2221 gattaaagtc gccgtcggca aagaaaacgt cgtcttcacc ctgctcaaag gcgagccgct
     2281 cgatttgcag gtgtatggca aagacatcac gctcgacggc agccacgccg ccgcgttgga
     2341 aaaataagga gggtgcaaaa tgacgtttac cgcagtcctc ttcgatctcg acggcgtgat
     2401 taccgatacc gccgaatacc actaccgcgc gtggaaaaaa ctcgccgaag aactgggcat
     2461 cggcatcgac cgcaagttta acgagcagct caaaggcgtg tcgcgcgacg attcgctcaa
     2521 acgcatcctc gcgcacggcg gtaaaaccgt cggcgaagcc gagtttgccg aactgacccg
     2581 ccacaaaaac gacaactacg tcgagatgat tcaggcagtc aaacccgaag acgtgtaccc
     2641 cggcattttg ccgctgctgg aagcattgaa agcaaacggc aaaaaaatcg ccctcgcatc
     2701 cgccagtaaa aacggcccgt tcctgctcga acgcatgggg ctgacccact tcttcgacgc
     2761 cattgccgac cctgccgccg tcgcgcattc caaacccgcc cccgacatct tcctcgccgc
     2821 cgccgagggc gtgggcgcag acatccgccg ctgcatcggc attgaagacg ctgccgcagg
     2881 tgtcgccgcc atcaaagccg ctggcgcctt acccatcggc gtgggcaaag ccgaagactt
     2941 gggcagcgac atcgcgctgg tgtccggcac cgccgagctg acctatgcct atttgcaaaa
     3001 cgtgtgggca cagtcgggca ggtaaaacgc gtcagataaa gtgttaagga agtaaaaggc
     3061 cgtctgaaca gtgtttcaga cggcctgaga cctttgcaaa attcctttcc ctcccgacag
     3121 ccgaaatcca aacacaggtt ttcggctgtt ttcggctgtt tccgccccag atacctccta
     3181 attctaccca aac
//
LOCUS       DAXHSF010000186         3178 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.186, whole genome shotgun sequence.
ACCESSION   DAXHSF010000186 DAXHSF010000000
VERSION     DAXHSF010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.186"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            112..2880
                     /gene="tdfH"
                     /locus_tag="ACFM8S_001883"
     CDS             112..2880
                     /gene="tdfH"
                     /locus_tag="ACFM8S_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent zinc piracy receptor TdfH"
                     /protein_id="HFC6314904.1"
                     /translation="MRSSFRLKPICFYLMGVMLYHHSYAEDAGRAGSEAQIQVLEDVH
                     VKAKRVPKDKKVFTDARAVSTRQDIFKSSENLDNIVRSIPGAFTQQDKSSGIVSLNIR
                     GDSGFGRVNTMVDGITQTFYSTSTDAGRAGGSSQFGASVDSNFIAGLDVVKGSFSGSA
                     GINSLAGSANLRTLGVDDVVQGNNTYGLLLKGLTGTNSTKGNAMAAIGARKWLESGAS
                     VGVLYGHSRRSVAQNYRVGGGGQHIGNFGAEYLERRKQQYFVQTGGLKFNSDSGKWER
                     DLQRQFWKTKWYKQYEDPQELQKYIEGQDKSWRENLAPQYDITPIDPSSLKQQSAGNL
                     FKLEYDGVFNKYTAQFRDLNTKIGSRKIINRNYQFNYGLSLNSYANLNLTAAYNSGRQ
                     KYPKGSKFTGWGLLKDFETYNNAKILDLNNTATFRLPRETELQTTLGFNYFHNEYGKN
                     RFPEELGLFFDGPDQDNGLYSYLGRFKGDKGLLPQKSTIVQPAGSQYFNTFYFDAALK
                     KDIYRLNYSTNTVGYRFGGEYTGYYGSDDEFKRAFGENSPTYKKHCNRSCGIYEPVLK
                     KYGKKRANNHSVSISADFGDYFMPFASYSRTHRMPNIQEMYFSQIGDSGVHTALKPER
                     ANTWQFGFNTYKKGLLKQDDILGLKLVGYRSRIDNYIHNVYGKWWDLNGNIPSWVSST
                     GLAYTIQHRNFKDKVHKHGFELELNYDYGRFFTNLSYAYQKSTQPTNFSDASESPNNA
                     SKEDQLKQGYGLSRVSALPRDYGRLEVGTRWLGNKLTLGGAMRYFGKSIRATAEERYI
                     DGTNGGNTSNFRQLGKRSIKQTETLARQPLIFDFYAAYEPKKNLIFRAEVKNLFDRRY
                     IDPLDAGNDAATQRYYSSFDPKDKDEDVTCNADKTLCNGKYGGTSKSVLTNFARGRTF
                     LMTMSYKF"
     gene            2910..3149
                     /locus_tag="ACFM8S_001884"
     CDS             2910..3149
                     /locus_tag="ACFM8S_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314905.1"
                     /translation="MPSESPSDGICFPKRIILPQAYANPFYRIGNSKKNPFHSHAGKP
                     VDFGYFWLFRVIYESSFPQKRESRHSMLRQFIEND"
BASE COUNT          904 a          788 c          765 g          721 t
ORIGIN      
        1 attgaaatct tcagacggta tatcaaattt acactttttt aatgtttatg ccgcctgaaa
       61 aaaatgctag tatatttcct aattgtctga ctgtttattg ttgaggaaaa tatgagatct
      121 tctttccggt tgaagccgat ttgtttttac cttatgggtg ttatgctata tcatcatagt
      181 tatgccgaag atgcagggcg cgcgggcagc gaggcgcaga tacaggtttt ggaagatgtg
      241 cacgtcaagg cgaagcgcgt accgaaagac aaaaaagtgt ttaccgatgc gcgtgccgta
      301 tcgacccgtc aggatatatt caaatccagc gaaaacctcg acaacatcgt acgcagcata
      361 cccggtgcgt ttacacagca agataaaagc tcgggcattg tgtctttgaa tattcgcggc
      421 gacagcgggt tcgggcgggt caatacgatg gtggacggca tcacgcagac cttttattcg
      481 acttctaccg atgcgggcag ggcaggcggt tcatctcaat tcggtgcatc tgtcgacagc
      541 aattttattg ccggactgga tgtcgtcaaa ggcagcttca gcggctcggc aggcatcaac
      601 agccttgccg gttcggcgaa tctgcggact ttaggcgtgg atgacgtcgt tcagggcaat
      661 aatacctacg gcctgctgct aaaaggtctg accggcacca attcaaccaa aggtaatgcg
      721 atggcggcga taggtgcgcg caaatggctg gaaagcggag catctgtcgg tgtgctttac
      781 gggcacagca ggcgcagcgt ggcgcaaaat taccgcgtgg gcggcggcgg gcagcacatc
      841 ggaaattttg gcgcggaata tctagaacgg cgcaagcagc aatattttgt acaaacaggc
      901 gggttgaaat tcaattccga cagcggaaag tgggaacgcg atttgcaaag gcaattctgg
      961 aaaaccaagt ggtataaaca atacgaagac ccccaagaac tgcaaaaata catcgaaggt
     1021 caagataaaa gctggcggga aaacctggcg ccgcaatacg acattacccc catcgatccg
     1081 tccagcctga agcagcagtc ggcaggcaat ctgtttaaat tggaatacga cggcgtattc
     1141 aataaataca cggcgcaatt tcgcgattta aacaccaaaa tcggcagccg caaaatcatc
     1201 aaccgcaatt atcaattcaa ttacggttta tctttaaact catatgccaa cctcaatctg
     1261 accgcagcct acaattcggg caggcagaaa tatccgaaag ggtcgaagtt tacaggctgg
     1321 gggcttttaa aagattttga aacctacaac aacgcgaaaa tcctcgacct caacaacacc
     1381 gccaccttcc ggctgccccg cgaaaccgag ttgcaaacca ctttgggctt caattatttc
     1441 cacaacgaat acggcaaaaa ccgctttcct gaagaattgg ggctgttttt cgacggtccg
     1501 gatcaggaca acgggcttta ttcctatttg gggcggttta agggcgataa agggctgctg
     1561 ccccaaaaat caaccatcgt ccaaccggcc ggcagccaat atttcaacac gttctacttc
     1621 gatgccgcgc tcaaaaaaga catttaccgc ttaaactaca gcaccaatac cgtcggctac
     1681 cgtttcggcg gcgaatatac gggctattac ggctcggatg acgaatttaa gcgggcattc
     1741 ggagaaaact cgccgacata caagaaacat tgcaaccgga gctgcgggat ttatgaaccc
     1801 gtattgaaaa aatacggcaa aaagcgcgcc aacaaccatt cggtcagcat tagtgcggac
     1861 ttcggcgatt atttcatgcc gttcgccagc tattcgcgca cacaccgtat gcccaacatc
     1921 caagaaatgt atttttccca aatcggcgac tccggcgttc acaccgcctt aaaaccagag
     1981 cgcgcaaaca cttggcaatt tggcttcaat acctataaaa aaggattgtt aaaacaagat
     2041 gatatattag gattaaaact ggtcggctac cgcagccgca tcgacaacta catccacaac
     2101 gtttacggga aatggtggga tttgaacggg aatattccga gctgggtcag cagcaccggg
     2161 cttgcctaca ccatccaaca ccgcaatttc aaagacaaag tgcacaaaca cggttttgag
     2221 ttggagctga attacgatta tgggcgtttt ttcaccaacc tttcttacgc ctatcaaaaa
     2281 agcacgcaac cgaccaactt cagcgatgcg agcgaatcgc ccaacaatgc gtccaaagaa
     2341 gaccaactca aacaaggtta tgggttgagc agggtttccg ccctaccgcg agattacgga
     2401 cgtttggaag tcggtacgcg ctggttgggc aacaaactga ctttgggcgg cgcgatgcgc
     2461 tatttcggca agagcatccg cgcgacggct gaagaacgct atatcgacgg caccaatggg
     2521 ggaaatacca gcaatttccg gcaactgggc aagcgttcca tcaaacaaac cgaaaccctt
     2581 gcccgccagc ctttgatttt tgatttttac gccgcttacg agccgaagaa aaaccttatt
     2641 ttccgcgccg aagtcaaaaa tctgttcgac aggcgttata tcgatccgct cgatgcgggc
     2701 aatgatgcgg caacgcagcg ttattacagc tcgttcgacc cgaaagacaa ggacgaagac
     2761 gtaacgtgta atgctgataa aacgttgtgc aacggcaaat acggcggcac aagcaaaagc
     2821 gtattgacca attttgcacg cggacgcacc tttttgatga cgatgagcta caagttttaa
     2881 aggcagcccg cgttttgtgg aaaaccgcaa tgccgtctga aagcccttca gacggcattt
     2941 gtttccccaa acgcatcatc ctgccgcaag cctatgccaa tccgttttat cgcatcggca
     3001 actcaaagaa aaatccattt cattcccacg cagggaagcc ggttgatttc ggttattttt
     3061 ggttgtttcg ggtaatttat gagtcgtcat tcccgcaaaa gcgggaatct agacattcaa
     3121 tgctaaggca atttatcgaa aatgactgaa actcaaaaaa ctggattccc actttcgt
//
LOCUS       DAXHSF010000187         3099 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.187, whole genome shotgun sequence.
ACCESSION   DAXHSF010000187 DAXHSF010000000
VERSION     DAXHSF010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.187"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            270..893
                     /locus_tag="ACFM8S_001885"
     CDS             270..893
                     /locus_tag="ACFM8S_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFC6314906.1"
                     /translation="MKKPTDTLPVNLQRRRLLCAAGALLLSPLAHAGAQREETLADDV
                     ASVMRSSVGSVNPPRLVFDNPKEGERWLSAMSARLARFVPEEEERRRLLVNIQYESSR
                     AGLDTQIVLGLIEVESAFRQYAISGVGARGLMQVMPFWKNYIGKPAHNLFDIRTNLRY
                     GCTILRHYRNLEKGNIVRALARFNGSLGSNKYPNAVLGAWRNRWQWR"
     gene            1004..2386
                     /locus_tag="ACFM8S_001886"
     CDS             1004..2386
                     /locus_tag="ACFM8S_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC6314907.1"
                     /translation="MARDNRIQMFPHEWRASTTLSGVYALRMLGMFLVLPVLAVYAAS
                     LPGAEGNKTLVGLAMGIYGLTQALLQLPLGIASDKFGRKKTIYAGLVVFAAGSFLAAA
                     ADTLPMLVAARAIQGAGAVSAAVTALLADLTRDGVRTRAMAMIGLSIGLTFSVSLVVA
                     PVIADAVGVRGLFMLTGILTVISIGVVAWMTPDPEVSKLHEDTQAQPSRIGEVLKNRR
                     LLTLDFGIFALHAAQMALFTALPFAMTQLGLEKIQHWKVYLPSTITGLVVMVPLIIVG
                     ETRNKLKQVFVLGIVCIAAAQLGLLSGMRSVGLITAYLVVYFIGFNVLEASLPSMVSK
                     IAPSDLKGTAMGVYNTMQSLGLFAGGVAGGLLFQKYGFSGVFAFCSILMLLWLVIAVL
                     SPAPKPVKNLSYPVGGVWQGNQDGLQCALSQLEGVEDIGFSFDGQTVYLKVLQKGFDQ
                     AAAEKIITGV"
     gene            2389..2913
                     /locus_tag="ACFM8S_001887"
     CDS             2389..2913
                     /locus_tag="ACFM8S_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFC6314908.1"
                     /translation="MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQ
                     RVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRIQSRKYQGKDGIERTAYDIVANE
                     MKMLGGRNENSGGAPYEEGYGQSQEAYQRPAQQSRQPASDAPSHPQEAPAAPRRQPVP
                     AAAPVEDIDDDIPF"
BASE COUNT          628 a          834 c          933 g          704 t
ORIGIN      
        1 ctgctgccgc ccgaaaagcg tgatgcgctg atggtgctgc tgcacaatcc ggacgacgga
       61 tagccggtga aaacgggaaa tgcccgggtg gggttgtctg tgcctgtttg gacaagcgtt
      121 cttgagagcg gtaaaatcaa gatttgcctg aaaagggcag ggcgatatgc cgtgcttgga
      181 ttttgtgcgc ggtttttcgc gttgaaagca attatgtctg tttgaatatt taatgccgtc
      241 tgaaacgtcc ggacgcgccc ccgttcccta tgaaaaaacc gaccgatacc ctacccgtta
      301 atctgcaacg ccgccgcctg ttgtgtgccg ccggtgcgtt gttgctcagt cctctggcgc
      361 acgccggcgc gcaacgtgag gaaacgcttg ccgacgatgt ggcttccgtg atgaggagtt
      421 ctgtcggcag cgtcaatccg ccgaggctgg tgtttgacaa tccgaaagag ggcgaacgtt
      481 ggttgtctgc catgtcggca cgtttggcaa ggttcgtccc cgaggaggag gagcggcgca
      541 ggctgctggt caatatccag tacgaaagca gccgggccgg tttggatacg cagattgtgt
      601 tggggctgat tgaggtggaa agcgcgttcc gccagtatgc aatcagcggt gtcggcgcgc
      661 gcggcctgat gcaggttatg ccgttttgga aaaactacat cggcaaaccg gcgcacaacc
      721 tgttcgacat ccgcaccaac ctgcgttacg gctgtaccat cctgcgccat taccggaatc
      781 ttgaaaaagg caacatcgtc cgcgcgcttg cccgctttaa tggcagcttg ggcagcaata
      841 aatatccgaa cgccgttttg ggcgcgtggc gcaaccgctg gcagtggcgt tgattttgaa
      901 cccgcgccgc aaccgaaata cggcgaatcc tgtataatcc gaaaatctgt tcactggaag
      961 ttcagacggc attgcaactg ttgatgccgt ctgaaaaaat atgatggcaa gagacaaccg
     1021 catccaaatg tttccgcacg aatggcgcgc cagtacgacg ctttccggcg tgtacgcgct
     1081 gcgtatgctg ggtatgttcc tcgtgctgcc cgtattggcg gtgtatgccg cctcgctgcc
     1141 cggcgcggaa ggcaacaaaa cgctggtcgg gctggcaatg ggcatttacg ggctgacaca
     1201 ggctctgctg caactgcctt tgggcatcgc ttccgacaag ttcgggcgca agaaaaccat
     1261 ttatgcggga ctggtcgtgt ttgcggcggg cagctttctt gccgccgccg ccgatacgct
     1321 gcccatgctg gtcgccgcac gcgccataca gggtgcaggg gcggtcagtg cggcggttac
     1381 cgcgctgctg gcggatttga cgcgcgacgg cgtacgtacc cgcgcaatgg cgatgatcgg
     1441 tttgagtatc ggtttgacgt tttcggtcag cctcgtcgtt gcccccgtga ttgccgacgc
     1501 ggtcggcgtt cgcggactgt ttatgctgac cggcattctg accgtcatca gcatcggcgt
     1561 ggtggcgtgg atgactcccg atcccgaagt ttccaagctg cacgaagata cgcaggcgca
     1621 gccttcgcgc ataggcgaag ttttgaaaaa ccgtaggctg ctgacgcttg atttcggcat
     1681 tttcgccctg cacgccgcac aaatggcatt gtttaccgcg ctgcctttcg cgatgaccca
     1741 gctcggtttg gaaaaaatac agcattggaa agtctatctg ccttcgacca ttacgggctt
     1801 ggtggtgatg gttccgctga ttatcgtcgg cgagacgcgc aacaagctta agcaggtttt
     1861 tgttttgggt atcgtctgta ttgcggcggc gcagctcggt ttgctgtccg gtatgcgctc
     1921 ggtaggcttg attaccgctt atttggttgt ttactttatc ggttttaatg tgttggaagc
     1981 gagcctgccg tctatggttt ccaaaatcgc gccgtccgac ctgaagggta cggcgatggg
     2041 cgtgtacaac acgatgcagt cgctcggact gtttgccggc ggcgtggcag gcggtttgct
     2101 gtttcaaaaa tacggctttt ccggcgtgtt tgccttttgc agcatattga tgctgctgtg
     2161 gctggtgatt gccgttttat cgcctgcgcc caagcccgtc aaaaacctca gttaccctgt
     2221 cggcggcgtg tggcagggca atcaggacgg cttacaatgc gccttgtcgc aactcgaagg
     2281 cgtggaagac atcggtttca gtttcgacgg gcagaccgtc tatctcaaag tgttgcagaa
     2341 gggtttcgat caggctgccg ctgaaaaaat cattacagga gtttaaaaat gtcattgaac
     2401 aaagttatcc ttatcggccg cctcggacgc gatcccgaag tgcgctatat gcccaacggc
     2461 gaggcggtgt gtaatttcag cgtcgccacc agcgaaactt ggaacgaccg caacggccaa
     2521 cgtgtagagc gtactgagtg gcacaacatc acgatgtacc gcaaactggc ggaaattgcc
     2581 gggcaatacc tcaaaaaagg cgggctggtt tatctggaag gcagaatcca aagccgcaaa
     2641 taccaaggca aagacggcat cgaacgcacc gcttacgata ttgtcgccaa cgaaatgaaa
     2701 atgttgggcg ggcgcaatga aaacagcggc ggtgcgcctt acgaggaagg ttacggtcag
     2761 agtcaggagg cttaccaacg ccccgcacag caaagccggc agcccgcctc cgacgcgccg
     2821 tcccatcccc aagaagcacc agccgcgccg cgccgccaac ccgtgcctgc cgccgccccg
     2881 gtcgaggaca ttgacgacga tatcccgttc tgaattttac ggccggacat cctcgcggag
     2941 ggaaatcata aaggacggag aaaccttaaa ccttacgggg cagggtttct cttttttgca
     3001 tttgggccgg cttgttgccg ccctttgcgt tgggcggaac gttgcgattg cgccgttcgg
     3061 gcgttttgag gcagtgatgc ctgccgcacg tccccgctg
//
LOCUS       DAXHSF010000188         3088 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.188, whole genome shotgun sequence.
ACCESSION   DAXHSF010000188 DAXHSF010000000
VERSION     DAXHSF010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.188"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(53..547)
                     /locus_tag="ACFM8S_001888"
     CDS             complement(53..547)
                     /locus_tag="ACFM8S_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HFC6314909.1"
                     /translation="MKKTLLAIIAVFALSACRQAEEGPPPLPQQISDRSVGHYCSMNL
                     TEHNGPKAQIFLNGKPDQPVWFSTIKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNP
                     NADTEWMDAKKAFYVIDSGFIGGMGAEDALPFGNKEQAEKFAKDKGGKVVGFDDMPDT
                     YIFK"
     gene            complement(544..675)
                     /locus_tag="ACFM8S_001889"
                     /pseudo
     CDS             complement(544..675)
                     /locus_tag="ACFM8S_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688339.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(692..1306)
                     /locus_tag="ACFM8S_001890"
                     /pseudo
     CDS             complement(692..1306)
                     /locus_tag="ACFM8S_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709597.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(1364..2563)
                     /locus_tag="ACFM8S_001891"
     CDS             complement(1364..2563)
                     /locus_tag="ACFM8S_001891"
                     /inference="COORDINATES: protein motif:HMM:TIGR04247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HFC6314910.1"
                     /translation="MASGVYQTKLHIDKPITIEGPADRSATIEGDRSGRTIAVHAPDV
                     TLRNLTITRSGMSLPAMDAGIYLEETAPRALIEHNNILDNSVGVYLHGSADAMVRENK
                     IVGDATLRVNERGNGVTVWNAPGAQVVGNDISKGRDGIFSNTSTHNTYKNNRFSDLRF
                     AVHYMYTNDSEVSGNISVGNNMGYVLMFSERLKVFDNIAVGSRDQGIMLNYVNYSDIH
                     DNIINKAGKCVFAYNANYDKLSANHFENCQIGIHFTAAIEGTSLHDNSFINNESQVKY
                     VSTRFLDWSEGGHGNYWSDNSAFDLNGDGFGDSAYRPNGIIDQIIWRAPVSRLLMNSP
                     AISIVKWAQAQFPAVLPGGVVDSKPLMKPYAPKIQTRYQAMKDGLLKEVETRQSEWGR
                     AENGSLN"
     gene            complement(2452..2973)
                     /locus_tag="ACFM8S_001892"
     CDS             complement(2452..2973)
                     /locus_tag="ACFM8S_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosR"
                     /protein_id="HFC6314911.1"
                     /translation="MSCFSIKRMSAFRARITAFFAAFVFLTAALPAYAERLPDFLAKI
                     QPSEIFPGADRYGKPEGKPMVARVYKGDEQLGLVYITTDVVNTRGYSSKPIDTLMALA
                     NDGTIAGAKLVDHHESIMLIGIPHLPAPGRAIRSNWLPAYIKPNFTSTNRLRLKGLPT
                     VLQPSKGTGAVVR"
BASE COUNT          682 a          816 c          820 g          770 t
ORIGIN      
        1 aaatgccgtc tgaaccttgg cttcaacgtt cagacggtat ttttgtttgg atttatttga
       61 aaatataggt atcaggcata tcgtcgaaac cgacaacctt accgccttta tcctttgcaa
      121 atttttcagc ctgctccttg ttgccgaacg gtagcgcgtc ttccgcaccc ataccgccga
      181 taaagccgct gtcgatgacg taaaaggctt ttttcgcatc catccactcc gtgtcggcat
      241 tgggattcgt ccaatcggta acattgccca tatcggtaac gtaaatcacg cggatgcctt
      301 tgggttcttc gggcagcttg gtatagccga acatctgctt gatggtggaa aaccaaacgg
      361 gctgatcggg tttgccgttc aaaaaaatct gggctttggg gccgttgtgt tcggtcagat
      421 tcatactgca atagtgtccg accgaacggt cgctaatctg ctggggtaaa ggcggcggcc
      481 cctcttccgc ctgccggcag gcacttaagg cgaaaacggc aataattgcc aacagggttt
      541 tcttcatatt tgtcgctttc taaaaattcc ggctgccaaa acaagcggaa tgataaccca
      601 taaaacctgc gcggtcagca aaacggggac ggtcaggctg atttgtccgc tcaaacccgc
      661 catacccgca tatagtggat taaatttaat ccacgataat gcggtcggcg tgcccgtcca
      721 actcggcaag ggcgtgggtg ctgagcaata cggtcgcgcc gcgcccgttg agttcgcgca
      781 cgacttcgta aaacatttgt cgtgatgcag ggtcaagacc ggttgtcggt tcatcaaaca
      841 gcaggacttt gggctcgccc agcagggctt gtgccaaggc aaggcgttgg cgcatccctt
      901 tagaataagt gccgacgcgg cggtgtgccg cctgtgaaat gccgacgcgc tcaagcagcc
      961 cccggttctg cgtgagcggc tgttttttaa gtttggcata aaaatccagc gtttcgatgc
     1021 cgatcagcga agggtgcagc gcaacggttt cgggcaggta gccgatttgg ctgcgaagcc
     1081 gcgcccccgc tttgctaccg gtacgttcgc ccaaaagcat cacttcgcct tcggtcgggg
     1141 taatcagccc gagtatcagc ttcataatgg tggacttgcc cgcgccgttg tgtccggcaa
     1201 gcccgacgct ttctcctgcc ttcaaaacca aatcgacttg gttgacggct ttttgcgccc
     1261 cgaaccgttt ggttaccttc ctcaattcga catgatgtgt ggtcatggtt gttccttttg
     1321 tgttttcaga cggcatttga atccggatgc cgtctgaagc agactagttc aaagaaccgt
     1381 tttccgccct gccccattcc gactgccgcg tttcgacttc tttgagtagc ccgtccttca
     1441 tcgcctgata acgggtttga attttggggg cataaggctt catcagcggt ttgctgtcca
     1501 ccacgccgcc aggcaaaacg gcgggaaatt gcgcctgcgc ccatttgacg atgctgattg
     1561 cgggactgtt catcaagaga cgcgatacgg gcgcgcgcca gatgatttgg tcgatgatgc
     1621 cgttgggccg gtacgcgctg tcgccgaagc cgtcgccgtt caaatcgaac gcgctgttgt
     1681 cgctccaata gttgccgtgt ccgccctcgc tccagtcgag aaagcgcgtg ctgacgtatt
     1741 tgacctggct ttcgttgttg ataaaggaat tgtcatgcag ggacgtgcct tcgatggcgg
     1801 cggtaaagtg tatgccgatt tggcagtttt caaaatgatt ggcggacagt ttatcgtagt
     1861 tggcattgta ggcaaaaacg cacttgcccg ccttgttgat aatgttgtcg tgaatatcgg
     1921 aatagttgac atagttgagc ataatgccct gatcgcggct gccgacggcg atattgtcaa
     1981 acactttgag ccgctcggaa aacatcagca catagcccat attgttgccc acggaaatat
     2041 tgccgctgac ttcgctgtcg ttggtgtaca tatagtggac ggcgaaacgc aaatcgctga
     2101 agcggttgtt tttgtaggtg ttgtgcgtgc tggtattgga aaaaatgccg tcccgccctt
     2161 tggaaatgtc gttgccgacg acctgcgcac cgggtgcgtt ccaaacggta acgccgttgc
     2221 cgcgctcgtt cacgcgcaaa gtcgcgtcgc cgacgatttt attctcgcgc accatcgcat
     2281 cggcagaacc gtgcagatag acgccgaccg aattatccaa aatattgttg tgttcaatca
     2341 gggcgcgcgg ggcagtttct tcgagataaa taccggcatc cattgcgggc aggctcatac
     2401 cggaacgtgt aatggtcagg ttgcggagcg ttacgtccgg cgcatgcaca gctatcgtac
     2461 gaccgctcct gtccccttcg atggttgcag aacggtcggc aggcccttca atcgtaatcg
     2521 gtttgtcgat gtgaagtttg gtttgatata cgccggaagc cagtttgagc gtatcgcccg
     2581 cccgggcgcg ggcaaatgcg ggataccgat cagcataatc gactcatggt gatcaaccaa
     2641 tttcgccccg gctatcgtgc cgtcgtttgc cagcgccatc agcgtatcaa tcggcttgct
     2701 cgaataaccg cgcgtattga ccacatcggt tgtgatatag accaaaccga gctgctcatc
     2761 gcctttgtaa acgcgggcaa ccataggctt accttccggc ttgccgtaac ggtccgcacc
     2821 cggaaaaatt tccgaaggct gtattttcgc cagaaaatca ggcagacgct ccgcataagc
     2881 gggcagtgcc gccgtcaaaa agacaaaggc ggcaaaaaac gccgttatcc gcgcccgaaa
     2941 cgcggacata cgtttgattg aaaaacagga catgactatc cccgatatac cgatactctt
     3001 taatattgat attatatgaa tcatctatcg gcgatttttt gatgcaaatc aaatttacaa
     3061 atcttaaaac caatccgcat acgggata
//
LOCUS       DAXHSF010000189         2968 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.189, whole genome shotgun sequence.
ACCESSION   DAXHSF010000189 DAXHSF010000000
VERSION     DAXHSF010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.189"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            123..1667
                     /gene="purF"
                     /locus_tag="ACFM8S_001893"
     CDS             123..1667
                     /gene="purF"
                     /locus_tag="ACFM8S_001893"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFC6314912.1"
                     /translation="MCGVLGLVSHEPVNQFLYDGLQMLQHRGQDAAGIATAEGGTFHM
                     HKGKGMVREVFRTRNMRDLTGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHN
                     GNLTNTAELYENVCNKHLRHVNTSSDSEVLLNVFAHELRREVSKNADPHRLNADNIFN
                     AVAQVHRLVRGAYGVIAMIAGYGMLAFRDPYGIRPLVLGSQTDSEGRKSYAVASESVA
                     FNALTYDLERDIRPGEAVFVGFDGTMIARQCSDRAKLSPCLFEYVYFARPDSVIDGVS
                     VYQSRLDMGVSLAEKIKRELPVDGIDVVMPIPDTSRPSAMELAVHLDKPYREGLIKNR
                     YIGRTFIMPGQATRKKSVRQKLSPMETEFAGKSVLLVDDSIVRGTTSREIVEMVRAAG
                     ARKVYIASAAPEVRYPNVYGIDMPTREELIANGRSAAEIAAEIGADGIVFQDLGDLEA
                     VVKALNPKIESFDSSCFNGIYQTGDIDDAYLDRLSAEKSGCGGLKIHPSRMEHSISIS
                     DAGDEE"
     gene            1767..2258
                     /gene="greB"
                     /locus_tag="ACFM8S_001894"
     CDS             1767..2258
                     /gene="greB"
                     /locus_tag="ACFM8S_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFC6314913.1"
                     /translation="MSTETKNYITPAGWQALKDELYQLVNKERPEIVQIVNWAAGNGD
                     RSENGDYLYGKRRMREIDRRIRFLTKRLESAVVVDPELREATDQVFFGATVGLLRGDG
                     REQTVKIVGIDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
                     KID"
     gene            2317..2943
                     /locus_tag="ACFM8S_001895"
     CDS             2317..2943
                     /locus_tag="ACFM8S_001895"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HFC6314914.1"
                     /translation="MRKIRTKICGITTPEDASAAVAAGADAVGLVFFQGSSRAVDIAR
                     AKKITAALPPFVSVVALFVNESAQNIRRILAEVPIHIIQFHGDEDDTFCRQFHRPYIK
                     AIRVQTTSDIRNAADRFPDAQALLFDAYHPSEYGGTGHRFDWTLLAEYSGKPWVLAGG
                     LTPENVGEAVRITGAEAVDVSGGVEASKGKKDPAKVAAFIATANRLSR"
BASE COUNT          712 a          907 c          772 g          577 t
ORIGIN      
        1 cttattctcc ataaacccac ctatctgtcc ttcgctcaaa tattgtcaac aattcaggca
       61 aaaaaccacc gtttacttta taattccgtt ttacccttaa acctcgaatc ggagaagacg
      121 atatgtgcgg cgtattaggt ttggtcagtc atgaacccgt gaaccagttt ctgtacgacg
      181 gcttgcagat gttgcagcac agggggcagg atgcggcggg cattgcaacg gcggaaggcg
      241 gtaccttcca tatgcacaaa ggcaaaggca tggtgcgcga agtgttccgc acacgcaata
      301 tgcgcgattt gaccggcaac gccggtatcg cccacgtccg ttatcctact gcgggcaacg
      361 caggcagcag cgcggaggcg cagcctttct acgtcagctc gccgttcggc atcgttttgg
      421 cgcacaacgg caacctcacc aacactgccg aactgtatga aaacgtgtgt aacaaacacc
      481 tgcgccacgt caacaccagc tccgattccg aagtcttgct caacgtattc gcgcacgaat
      541 tacgccgcga agtctctaaa aacgccgacc cgcaccggct caatgccgac aatattttca
      601 acgccgttgc ccaagtccac cgcctggtgc gcggcgcata cggtgtgatt gccatgattg
      661 cgggctacgg tatgctcgcc ttccgcgacc cttacggcat ccgcccgctg gtattgggtt
      721 cgcaaaccga cagcgaaggc agaaaatcct atgccgtcgc ctccgaatcc gtcgccttca
      781 acgcgcttac ctacgatttg gaacgcgaca tccggccggg cgaagcggta tttgtcggct
      841 ttgacggcac aatgattgcc cgccaatgca gcgaccgtgc caaactcagc ccctgccttt
      901 ttgaatatgt ttattttgcc cgccccgact ctgtgattga cggcgtatcc gtttaccaat
      961 cgcgcttgga tatgggcgtg tcgctggcgg aaaaaatcaa acgcgagctg cccgtggacg
     1021 gcatcgatgt cgtgatgccc attcccgaca ccagccgccc cagcgcgatg gagcttgccg
     1081 tccatctcga caaaccctac cgcgagggtt tgattaaaaa ccgctatatc ggccgcacct
     1141 ttattatgcc cggacaggcg actcgcaaaa aatccgtgcg ccaaaaactc agcccgatgg
     1201 aaaccgaatt tgcaggcaaa agcgtgttgc tggtggacga ctccatcgtg cgcgggacga
     1261 ccagccgcga aatcgtcgaa atggtacgcg cggcgggcgc gcgcaaagtc tatatcgcct
     1321 ccgccgcgcc cgaagtgcgc tatcccaatg tgtacggcat tgatatgccc acgcgcgaag
     1381 agttgattgc caacgggcgc agcgcggcgg aaatcgccgc cgaaatcggc gcggacggca
     1441 tcgtatttca agacttgggc gatttggaag ccgtcgtcaa agcactcaat ccgaaaatcg
     1501 aatccttcga ttcgtcctgt ttcaacggca tttatcagac cggcgacatc gatgatgcct
     1561 acctcgaccg cctgtctgcc gagaaatccg gctgcggcgg tctgaaaatc cacccgagca
     1621 ggatggaaca cagcatcagc atcagcgatg cgggcgacga agaataaggc ttcccaaacc
     1681 gcgatgccgt ctgaaatccg aacgccgggt tttcagacgg catttcccat atcggcatca
     1741 catttcccgt ttcatccgca cacaatatga gtaccgaaac caaaaactac attacgcccg
     1801 ccggatggca ggcattgaaa gacgagcttt atcaactggt caacaaagaa cgccccgaaa
     1861 tcgtccaaat cgtcaactgg gcggcaggca acggcgaccg cagcgaaaac ggcgactatc
     1921 tttacggcaa acgccgtatg cgcgaaatcg accgccgcat ccgtttcctg accaagcgtt
     1981 tggagtccgc cgtcgtcgtc gatcccgaat tgcgcgaagc gaccgaccaa gtatttttcg
     2041 gcgcgaccgt cggcctgttg cgcggcgacg ggcgcgagca aaccgtcaaa atcgtcggca
     2101 tcgatgaaat cgataccgcg caaaacaaaa tttcttggat ttcgccgttg gcgcgttgcc
     2161 tgattaaagc gcgcgaaggc gacgaagtgg ttttgaacac gccggaaggg cgtgaggaaa
     2221 ttgaaatcct ttctgtcgaa tacatcaaaa tcgactgatc gggaatgccg tctgaaacgg
     2281 tgcaagcgcg ccgtttccca aataaaggaa taccgaatga ggaaaatccg caccaaaatc
     2341 tgcggcatca ccacaccgga agacgcatcg gccgccgtag cggcaggtgc agatgccgtc
     2401 gggctggtct ttttccaagg cagcagccgg gccgtcgata ttgcccgcgc caaaaaaatc
     2461 accgccgcac tgccgccgtt tgtcagcgtt gtcgcccttt tcgtcaacga aagcgcgcaa
     2521 aacatccgcc gcatccttgc cgaagtgccg atacacatca tccaattcca cggcgacgaa
     2581 gacgacacat tctgccgtca gttccaccgc ccctatatca aggccattcg tgttcagacg
     2641 acatcagaca tccgaaacgc cgccgaccgc ttccccgacg ctcaggcact gcttttcgat
     2701 gcctaccatc cttcggaata cggcggcacc ggacaccgct tcgactggac gctgctggcg
     2761 gaatattcgg gcaaaccgtg ggtacttgcc ggcgggctga cccctgaaaa cgtcggcgaa
     2821 gccgtccgca tcaccggagc ggaagcggtc gatgtatccg gcggcgtgga agcgtctaaa
     2881 ggcaaaaaag acccagccaa agtcgccgcc tttatcgcaa ccgccaaccg cctatcccgt
     2941 taaagcaaca aaaattgccg ccggaatg
//
LOCUS       DAXHSF010000190         2950 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.190, whole genome shotgun sequence.
ACCESSION   DAXHSF010000190 DAXHSF010000000
VERSION     DAXHSF010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.190"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            163..1275
                     /gene="nadA"
                     /locus_tag="ACFM8S_001896"
     CDS             163..1275
                     /gene="nadA"
                     /locus_tag="ACFM8S_001896"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFC6314915.1"
                     /translation="MQTAARRSFDYDMPLIQTPTSACQIRQAWAKVADTPDHETAGRL
                     KDEIKALLKEKNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEAGTLVVAG
                     VRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVVVYANTSAA
                     VKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYIRRETGADMLLWQGSCIVH
                     NEFKGQELAALKAEHPDAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSRPEKKFIVA
                     TDLGILHEMQKQAPDKEFIAAPTAGNSGSCKSCAFCPWMAMNSLGGIKYALTSGHNEI
                     LLDRKLGEAAKLPLQRMLDFAAGLKRGDVFNGMGPA"
     gene            1327..2835
                     /gene="nadB"
                     /locus_tag="ACFM8S_001897"
     CDS             1327..2835
                     /gene="nadB"
                     /locus_tag="ACFM8S_001897"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFC6314916.1"
                     /translation="MQTDCDVLIAGNGLAALTLALSLPESFRIVILCKNRLDDTASRH
                     AQGGIAAAWSGEDDIEKHVADTLEAGAGLCDEAAVRTILSQGKPAIEWLLSQGVAFDQ
                     NNNGLHLTREGGHTCRRIAHVADYTGEAVMQSLIAQIRRCPNIRVYERQMALDIQTES
                     GAAYGLTVLDCRTQETYRIRARHTVLAGGGLGQIYAATTTPPECTGDAIAMAIRAGCA
                     VENLEFIQFHPTGLARPSENGRTFLISEAVRGEGGILTNQSGERFMPHYDLRAELAPR
                     DIVARDIAAEIAKQTQDFVSLDISHQPAAFVRQHFPSIHRHCLSQCGLDITRQAIPVR
                     PVQHYTCGGIQTDPCGRTSLPQLYALGETACTGLHGANRLASNSLLECVVTARLAAQT
                     IADGQAFQTVPSQRSSEHPSAEAGIFSDGIQSAFSRPVLQAFNQRHLGILRNDTGLHR
                     AIAQLRLWKQNQTEPHTASEYENRNLLECSLAVAQAAYRRRQNIGAHFNSDC"
BASE COUNT          691 a          905 c          809 g          545 t
ORIGIN      
        1 ccaaagcggt ttgtcggaac gaagcgggtc ggttttacgg tatcgcaaac ctgttttcag
       61 acgaccttgc ttattatact caagttgagc ataatataaa acagtttgaa aagcagacgg
      121 caaaccttca tgccgtctga aaaccagcta caaggaaaca tcatgcaaac cgccgcccgc
      181 cgctcgttcg actacgatat gcccctcatc caaacgccga cttccgcctg ccaaatccgt
      241 caggcgtggg cgaaggttgc cgatacgccc gaccacgaga cggcaggccg tctgaaagac
      301 gaaatcaagg ctttgctgaa ggagaaaaac gcggtcttgg tggcgcatta ttacgtcgat
      361 ccgctgattc aggatttggc tttggaaacg ggcggatgcg tgggcgattc gctggaaatg
      421 gcgcgcttcg gtgcggaaca cgaagccggt acgctggtgg tggctggtgt gcgcttcatg
      481 ggcgagagcg cgaaaatcct ctgccctgaa aaaacggtgc tgatgcctga tttggaggcg
      541 gaatgttctt tggatttggg ttgtcccgaa gaagcgtttt cagcgttttg cgaccaacac
      601 cccgaccgca cggtggtggt gtacgccaac acttccgccg ccgtgaaagc gcgtgccgac
      661 tgggtggtaa cgtcttcggt ggcgttggaa atcgtgtcgt atttgaaatc gcgcggcgag
      721 aaactgattt ggggacccga ccgccacctc ggcgactaca tccgccgcga aacgggcgcg
      781 gatatgctgt tgtggcaggg ttcgtgcatc gtccataacg aattcaaagg gcaagagctg
      841 gcggcgttga aggcggaaca tcccgacgcg gtggtgctgg ttcatcccga atcgccgcaa
      901 agcgtcatcg aactgggcga cgtggtcggc tcgaccagca aactgctcaa agccgccgta
      961 tcgcgtcctg aaaaaaaatt catcgtggcg accgatttag gcatcctgca cgaaatgcaa
     1021 aagcaggcgc ccgacaaaga atttatcgcc gcaccgacgg cgggcaacag cggaagctgc
     1081 aaaagctgcg cgttctgccc gtggatggcg atgaattcgc tgggcggcat caaatacgcc
     1141 ctgacaagcg ggcacaacga aatcctgttg gacagaaagc tgggcgaagc cgccaaactg
     1201 cctttacagc gtatgctcga cttcgcggca ggactcaaac gcggggatgt gttcaacggc
     1261 atgggccccg cctgatttgc cgtcaatatt gccgtttcag acgacctctc aaacaaggac
     1321 aacaccatgc aaaccgattg cgacgtattg attgccggaa acgggctggc ggcactgacg
     1381 ctcgccctgt cgctgcctga atcgttccgc atcgtcattt tgtgcaaaaa ccggctggac
     1441 gacaccgcca gccgtcatgc gcaaggcggg attgcggcgg cgtggtcggg agaggacgac
     1501 atcgaaaaac acgttgccga taccttggaa gctggcgcgg gtttgtgcga cgaagccgcc
     1561 gtccgcacca tcctgtcgca gggcaaaccg gcaatcgaat ggctgctgtc gcagggcgtg
     1621 gcgttcgacc aaaataataa cggcctgcac ctgacacgcg aaggcgggca tacctgccgc
     1681 cgaatcgccc acgtcgccga ttacacgggc gaagccgtca tgcagagcct gattgcccaa
     1741 atacgccgct gcccgaacat ccgcgtttac gagcggcaga tggcgttgga catccaaacc
     1801 gaatcaggcg cagcgtacgg actgaccgtc ctcgactgcc gaacgcaaga aacctaccgc
     1861 atccgcgccc gccataccgt actcgcaggc ggcggcttgg gacagattta cgccgccacc
     1921 accacgccgc ccgaatgcac gggcgacgcc atcgccatgg caatacgcgc aggctgcgca
     1981 gtcgaaaacc tcgaatttat ccaattccac cccacaggct tggcaaggcc gtctgaaaac
     2041 ggacgcacct tcctgatttc cgaagccgtg cgcggcgaag gcggcattct gaccaaccaa
     2101 tcgggcgaac ggtttatgcc gcattacgac ctccgtgccg aactcgcacc gcgcgacatc
     2161 gttgcccgcg acatcgccgc cgaaattgcc aaacaaacgc aagacttcgt ctcgctcgat
     2221 atcagccatc aacccgcagc gttcgtccgt cagcatttcc cgtccatcca tcggcactgc
     2281 ctgtcccaat gcggcttgga catcacgcgc caagccatcc ccgtccgccc cgtgcaacac
     2341 tacacctgcg gcggcatcca aaccgaccct tgcggcagaa cctccctgcc gcagctctac
     2401 gccttaggcg aaaccgcctg cacagggctg cacggagcca accgcctcgc cagcaattcc
     2461 ctgctcgaat gcgtcgttac cgccagactt gccgcccaaa ccatcgcgga cggacaagca
     2521 ttccaaaccg taccgtccca aaggtcgtct gaacacccct ccgccgaagc aggcatcttt
     2581 tcagacggca tccaaagcgc attcagccgc cccgtcctgc aagccttcaa ccaacgccat
     2641 ctgggcatcc tccgcaacga taccggcctg caccgcgcca tcgcccaact gcggctttgg
     2701 aagcaaaacc aaaccgaacc gcacaccgcg tccgaatacg aaaaccgtaa cctgctcgaa
     2761 tgcagcctcg ccgtcgccca agccgcatac aggcggagac agaacatcgg cgcgcatttt
     2821 aatagtgatt gttaaggttg gctggttaga aaaaatcgtt caaatgaatt ttaatagcat
     2881 ggttaagaga ttggcattca gtttttaggg ttgcgtaagt tgggttatca atctgttcta
     2941 aaagcagaaa
//
LOCUS       DAXHSF010000191         2941 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.191, whole genome shotgun sequence.
ACCESSION   DAXHSF010000191 DAXHSF010000000
VERSION     DAXHSF010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.191"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(127..1437)
                     /locus_tag="ACFM8S_001898"
     CDS             complement(127..1437)
                     /locus_tag="ACFM8S_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFC6314917.1"
                     /translation="MTDLFAREPDAPLAERLRPHTLDDVVGQEHLIGEGKPLRVAVEG
                     GKPHSMLLWGPPGVGKTTLARILAQSFNAQFLPVSAVFSGVKDIREAIDKAEIALQQG
                     RATILFVDEVHRFNKAQQDAFLPHVESGLLTFIGATTENPSFEVNPALLSRAQVYVLQ
                     PLSSDDLKKLIAKVLALPEYQEFTIETDAQKLLVNTADGDARRLLNLLEQLLRAADTR
                     RLKNLTAEFLADSLGAQIRRFDKGGESFYNQISALHKSVRGSHPNAALYWFCRMLDGG
                     TDPRYLARRIVRIAWEDIGLADPRAFQIANDAAATFERLGSPEGELALAQAVLYLAAA
                     AKSNAGYKAYKQMRRFVAENSSDEVPVHLRNAPTKLMKELGYGREYRYAHDEPNAYAA
                     GESYMPDGLDEPDFYQPVPRGLEIKIGEKLEWLKSLDEEVLKAK"
     gene            1749..2171
                     /locus_tag="ACFM8S_001899"
     CDS             1749..2171
                     /locus_tag="ACFM8S_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314918.1"
                     /translation="MEWVSLFSDPQRDDDSLITLKDEKITVKNYIVPWWKKGENFRKL
                     ELITTGKNDNGNLTVNGIYGIQSFADSQLGYTPELLVKSNNDILFKNNLQSVDVKPHS
                     GNIHLDAKGKIQFNLNVPDVDGVQDVASGKKYRQTIVP"
     gene            2859..2941
                     /locus_tag="ACFM8S_001900"
                     /pseudo
     CDS             2859..2941
                     /locus_tag="ACFM8S_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248742.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
BASE COUNT          817 a          707 c          698 g          719 t
ORIGIN      
        1 ttaatccact ataccacgtt gtctttattg gctttatcaa taaacaggat aagacctgaa
       61 aaaaagccga tacgcctttt tggtgtaccg gctttgccat actgttctgc ttcagacagc
      121 attgcttcat tttgccttta atacttcttc gtccagcgat ttcaaccatt ccagcttttc
      181 gccgattttg atttccaacc cgcgcgggac gggttggtag aagtccggtt cgtccaagcc
      241 gtcgggcata tagctttcgc cggcggcgta ggcgttcggt tcgtcgtggg cgtagcggta
      301 ttcgcgtccg tagcccaatt ccttcattaa cttggtcggg gcgttgcgaa gatggacagg
      361 cacttcgtcg ctgctgtttt ctgcaacaaa tcggcgcatt tgtttatacg ctttgtagcc
      421 cgcgttggat ttcgcggcgg cagcaaggta cagcacggct tgagccaaag ccagttcgcc
      481 ttcgggcgag cctaagcgtt cgaaggtggc ggcggcatcg ttggcgattt ggaaggcgcg
      541 cgggtcggca agcccgatgt cttcccaagc gatacgcacg atgcggcggg cgaggtagcg
      601 cgggtcggtg ccgccgtcga gcatacggca gaaccaatac agcgcggcgt tcggatgcga
      661 accgcgcacg gatttgtgca gggcggagat ttggttgtag aagctctcgc cgcctttgtc
      721 gaaacggcgg atttgcgccc cgagactgtc ggcgagaaat tcggcggtta agtttttcag
      781 acgacgtgta tcggcggcgc gtaaaagttg ttccaacaaa ttcaacaatc tgcgcgcatc
      841 accgtcggcg gtattcacga gtaatttttg cgcatccgtt tcaatcgtaa actcttggta
      901 ttcaggcaaa gccaatacct tggcaatcag ctttttcagg tcgtctgaag acaagggttg
      961 caaaacatac acctgagcgc ggctcaacag cgcgggattg acttcaaacg acggattttc
     1021 cgtcgtcgca ccaataaagg ttagcaaacc gctttcgaca tgcggcaaaa acgcgtcctg
     1081 ctgcgccttg ttgaagcggt ggacttcatc gacaaacaaa atcgtcgcgc gtccctgctg
     1141 caaagcgatt tcggctttat cgattgcctc gcggatgtcc ttcacgccgg aaaatacggc
     1201 ggaaacaggc aaaaactggg cgttgaaact ctgcgccaaa atccgcgcca acgtcgtctt
     1261 gcccacgccc ggcggccccc acagcaacat agaatgcggc ttgccgcctt ctaccgccac
     1321 gcgcaaaggt ttaccttcgc cgatgaggtg ttcctgcccc accacgtcgt caagcgtatg
     1381 cggacgcaat cgttcggcaa gcggcgcgtc gggttctcgg gcaaacaaat cggtcataac
     1441 ggctccgtca acaggttttc aaacaatatg attatacggc agggaacggc ggcgtgccgc
     1501 atacggattc cgcccctccg tttgccttaa gccgatatta ggcgcatact ggaaaagacg
     1561 agagacttca cacaatatat ccggcacgga gaccgattcc gcatcggcat gacaataccc
     1621 aaatcagcgt ttcaattaaa cattaaggag actaaaatag aaaatttgct ttatctacca
     1681 ttgctttgtt gattcaatcg gcattatgtt ttgaggcgga agcccatgaa tatactaata
     1741 ttcaagagat ggaatgggtg tctttatttt ctgatccgca aagagacgat gatagtctta
     1801 taacccttaa agatgaaaaa atcactgtaa aaaactatat tgtgccttgg tggaaaaaag
     1861 gtgaaaactt tagaaaatta gaacttatta ccactggtaa aaatgataat ggaaatttaa
     1921 cggtaaatgg aatttacggc atccaatctt ttgcagactc tcaattggga tatacgcctg
     1981 aattattggt aaaaagcaat aatgatattc tatttaaaaa taatctccaa tctgttgacg
     2041 tgaagcccca ttccggaaat atccatttgg atgccaaagg aaaaattcaa tttaatttga
     2101 atgttcccga tgttgatggg gtacaagatg tagccagtgg aaaaaaatac cgacaaacaa
     2161 tagtgcctta gtgcggtatg tcaacactat cagccccccg atgaaaatga caaaggcagc
     2221 atatcctttc attcaaaaac aggcaatttg ttcaatatga acgttaaagt tcataaagac
     2281 acacaccatt tcggtattca caacctcaac aataccgatg ttcatctgac tgccgaagac
     2341 agcgataatg tgattgagat taaaggggaa ctcgccgacc ttatgccgac aggaaatgtt
     2401 gagagtacaa gttttttaag gggcattgaa acggctttta aatccaatac cgtattaacg
     2461 gggaataaca ataaaatatc catcgccact gagggcaagg gcttgggatt gggcgtaaat
     2521 ggtctccagt ccaactcccc ctattccgga cacacgacgg gcattttcat caataatcaa
     2581 tcaagcgacc ttacccaacc ttcccttgat ggcatcagca ccatcaaact caaagccgtc
     2641 aagggaaata atgaagttga tttaaatgta aaaaaccacg cctccgtaaa aggcattatc
     2701 gcgtcgcatt ctgcaaaagc aacgcttgaa gccgaagggg acaatattgt ccaggttaaa
     2761 aatccggaca ccaatacggc tttaatcgat gctttgaaaa aacaataccc caatgcttcg
     2821 aaagaactat acagggtggg ggttcaatcc acgactggaa cagtacgcat ttcgccaaac
     2881 ggcaaaatcg cattaacgga attgaaaact tttggaattg tacaaaacgg catttgcgtc
     2941 a
//
LOCUS       DAXHSF010000192         2937 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.192, whole genome shotgun sequence.
ACCESSION   DAXHSF010000192 DAXHSF010000000
VERSION     DAXHSF010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.192"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            31..1248
                     /locus_tag="ACFM8S_001901"
     CDS             31..1248
                     /locus_tag="ACFM8S_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC6314919.1"
                     /translation="MTHTASKTPKFWLTITAAAFILLITIGMRMTLGLFVQPVVNTTE
                     LSIAQFSLIIAVFQLMWGISQPLSGALADRFGAFRVLSGGAVLLVCACLIAPNIPTYW
                     GLMIAVGLLLAFGTGSGGFSIIMGQVAAQVPAHKRGLASGLVNAGGSAGQFLFAPLVQ
                     GLVVLPEVGWTGTFYVWGAIALLILPVSWWLAGGNNAAHTQHAQATHGQSLGEAVKTA
                     FKTPSYILLHLSFFACGFHIAFLVTHLPTEVALCGLPATVASTSIAIIGLANIAGCIF
                     SGWCTSRVRGKYVLFGLYASRAAMILIYIFSPKTDLNFYIFAAALGFTWLATVTPTAS
                     ITGKLFGTRYLATLFGLTMLSHQIGGFLGSYIGGIVITQFGDYGWMWYADALLAGTAA
                     LLNLPIREPRTAA"
     gene            1245..1376
                     /locus_tag="ACFM8S_001902"
     CDS             1245..1376
                     /locus_tag="ACFM8S_001902"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314920.1"
                     /translation="MKECRLKTLRRHFPNTPPVSGLHDTQISLIKPQTTAAIKPPFK"
     gene            complement(1451..1591)
                     /locus_tag="ACFM8S_001903"
     CDS             complement(1451..1591)
                     /locus_tag="ACFM8S_001903"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314921.1"
                     /translation="MNYRIGNAVCIDEMPDTGRGGQTFAKRTASDASPARAGAASDGI
                     LS"
     gene            1604..2401
                     /locus_tag="ACFM8S_001904"
     CDS             1604..2401
                     /locus_tag="ACFM8S_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFC6314922.1"
                     /translation="MKIVFITTVASSIYGFRAPVIKKLIGKNHQVYAFVSEFSDNELD
                     IIREMGVTPVTYRSNRSGLNPFSDIKSTFLIFKELKKISPDLVFPYFAKPVIFGTFAA
                     KLAGVPRIVGMLEGLGFAFTPQPESIPLKTKIIKGILIALYHIALPMLESLIVLNPDD
                     KDELTDKYGIKIKNIHILGGIGLDLRQYPYSEADIPDEKEPVKFLFIGRFLKEKGIDD
                     FIRAAEQVKDKYPDTVFTALGAIDKSRAGGAIWNALPPAILSVSPVL"
     gene            2398..2733
                     /locus_tag="ACFM8S_001905"
     CDS             2398..2733
                     /locus_tag="ACFM8S_001905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC6314923.1"
                     /translation="MNNVSEVIKEHHIFVLPSYYREGVPRSTQEAMAVGRAVITTDVP
                     GCRETVADKVNGFLIEPWNPRILAEKMIYFIENREAVRLMGNASYAIAKDKFDAEKVD
                     LKLLDILKA"
BASE COUNT          693 a          875 c          687 g          682 t
ORIGIN      
        1 cgcaaactta accgactcaa aatacacatt atgacgcaca ccgcatcgaa aacacccaaa
       61 ttttggctga ccatcaccgc cgccgcattc atcctgctga ttaccatcgg catgaggatg
      121 acgctcggac tgttcgtcca acccgtcgtc aacaccaccg aattgagcat tgcccaattc
      181 agcctcatca tcgccgtttt ccaactgatg tggggcatat cgcaaccatt gtccggcgcg
      241 cttgccgacc gtttcggcgc gttcagggta ttaagcggcg gcgcagtcct gctcgtctgc
      301 gcctgcctga ttgcccccaa catcccgact tattgggggc tgatgattgc cgtcgggctg
      361 ctgctcgcat tcggcacagg ctcaggcggt ttctccatca ttatggggca ggtcgccgcc
      421 caagtccccg cacacaaacg cggcttggca tccggactgg tcaacgccgg cggttcggca
      481 ggacaattcc tgttcgcacc gctggttcaa ggactcgtcg tcctgcccga agtcggctgg
      541 acgggcacat tttacgtttg gggcgcaatc gccctgctga ttctgcccgt ctcatggtgg
      601 cttgccggcg gcaacaacgc cgcccacacc caacacgccc aagccacaca cggacaaagc
      661 ctcggagaag cagtcaaaac cgccttcaaa accccaagct acatcctgct gcacctaagc
      721 ttttttgcct gcggcttcca catcgccttt ctcgtaaccc acctacccac ggaagtcgcc
      781 ctgtgcggac tgcccgccac cgtcgcctcg acatccatcg ccatcatcgg actggcaaac
      841 atcgccggct gcattttttc cggctggtgc acaagccgcg ttcggggcaa atacgtcctg
      901 ttcggactgt acgcctcgcg cgccgcgatg atactcatct acattttttc gcccaagacc
      961 gacctcaact tctacatttt cgccgccgca ctcggattta cctggctcgc caccgtcacg
     1021 ccgaccgcct ccattaccgg caaactcttc ggcacacgct acctcgccac cctgttcgga
     1081 ctgacgatgc tcagccacca aatcggcgga ttcctcggct cgtacatcgg cggcatcgtg
     1141 attacacaat tcggcgacta cggctggatg tggtacgccg acgcactgct ggcgggaacc
     1201 gccgccctcc tcaacctccc catccgcgaa ccccgaaccg ccgcatgaaa gaatgccgtc
     1261 tgaaaaccct cagacggcat tttccaaaca ccccgcccgt ttccggactg cacgacacgc
     1321 aaatcagcct tatcaaaccg caaacaaccg ccgcgattaa accgccattc aaataagtcc
     1381 gttttccctt cgggtgtaaa atcatcacaa aacgcgatac tgtatatcgc cgggcgttat
     1441 gcagcggatt tcaggataaa atgccgtctg aagccgcccc tgcccgggcg ggcgaagcgt
     1501 cggatgcggt gcgcttggca aaggtttgcc cgccgcgccc cgtatccggc atttcatcaa
     1561 tgcagaccgc atttccaatc ctataattca aggtgtccgc cttatgaaaa tcgtttttat
     1621 cacaacagtc gcatccagca tttacggttt ccgcgccccc gtcattaaaa aattaatcgg
     1681 caaaaaccat caggtgtatg cctttgtatc ggagttttcc gacaatgaac tggatattat
     1741 cagggaaatg ggggttacac ccgttaccta ccgttcaaac cgcagcgggc tgaacccgtt
     1801 ttccgatata aaatccacct tcctcatctt taaagaactc aaaaaaatat cgccggattt
     1861 ggttttccct tatttcgcaa aacccgtgat tttcggcact tttgccgcaa aactggcagg
     1921 cgtgcccaga atcgtcggga tgctggaagg tttgggattc gcatttaccc cgcagccgga
     1981 aagcataccg ttaaaaacaa aaatcataaa ggggattttg attgccttgt accacattgc
     2041 cctgccgatg ttggaaagcc tgattgtatt aaaccccgac gacaaagacg aactgacgga
     2101 caaatacggc atcaaaataa aaaacatcca tattttgggc ggaatcggtc tggatttgcg
     2161 gcaatatcct tattccgagg cggatattcc cgatgaaaaa gaacccgtaa aattcctctt
     2221 tatcggcaga tttctgaaag aaaaggggat tgatgatttt attcgggcgg cggaacaggt
     2281 taaggacaaa taccccgata cggtttttac cgctttgggc gcaatcgaca aatcacgcgc
     2341 ggggggggcg atttggaacg ccttgccgcc cgcgatatta tccgtttccc cggttttgtg
     2401 aacaatgttt ccgaagtgat aaaagaacat catatattcg tattgccgtc ttattatagg
     2461 gaaggcgttc cccgaagcac tcaggaggca atggccgtcg gcagggcagt gattacgacg
     2521 gatgtccccg gatgcaggga aacggtcgcc gacaaggtca acggcttcct gatcgaacct
     2581 tggaatcccc gcatcttggc cgaaaaaatg atttatttta tcgaaaacag ggaagccgtt
     2641 cgcctgatgg ggaatgcaag ttatgcgatt gccaaagata aattcgatgc cgaaaaagtc
     2701 gatttaaaat tactcgatat tttgaaggcg taaacaaggc tgcccgcttt tgagtttcgg
     2761 tcatttctga taaacctccg tcattcccgc cacacccccg tcattccctc gaaagcggga
     2821 attcaggttc gtttagtttc ggtcatttcc gataaattct tgcaactttg cgtttctaga
     2881 ttcccacttt cgtgggaatg acgacggagg gttgctgttt tcccgacaaa ttcccgc
//
LOCUS       DAXHSF010000193         2893 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.193, whole genome shotgun sequence.
ACCESSION   DAXHSF010000193 DAXHSF010000000
VERSION     DAXHSF010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.193"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            28..2178
                     /gene="dinG"
                     /locus_tag="ACFM8S_001906"
     CDS             28..2178
                     /gene="dinG"
                     /locus_tag="ACFM8S_001906"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFC6314924.1"
                     /translation="MLTDLEKNAIRDHYQNIGKNLPGFRPRASQREMIAAVANAFSRT
                     LAREEGGEPPKREGESIAVIEGPTGVGKSLAYLLAGGIMAQTRGKRLIVSSATVALQE
                     QLVDRDLPFLVEKSGLELTFALAKGRGRYLCPYKLYQLTQSNAQQNLLGFEAPAVLWD
                     SKPKPEELKLLRDIADEFSARRFNGDRDTWPEKIDDAIWLKVTNDRHGCLKTACPNRP
                     ECPFYLARDVLETVDVVVANHDLLLADISMGGGVILPAPENSFYCIDEAHHLPKKALS
                     RFAAEHSWNIAVWTLEKLPQLTGKIAALTDKAELANLADEAAASLLDSLHEWQFHLAE
                     EPSLSMGPSENDRRTNSEPTWLWEDGKIPEGLETTVSNTAIAARSLLKHVVGLNDALS
                     AARREKEQDGALLDRLTGDFGIFIARIEQISAVWDLLSTVPIEGEEPLAKWITRRADD
                     KNDYIFNASPISSASHLANSLWRRAAGAVLTSATLQSLGNFNLMLRQTGLQWLPETTT
                     LALKSPFDFEKQGELYIPPIYASPKDPEAHTAAIIEWLPKLISPIEAIGTLVLFSSRK
                     QMQDVALRLPGNYLPLLLVQGELPKAVLLQKHHRAIEEGKASIIFGLDSFAEGLDLPG
                     TACVQVIIAKLPFAMPDNPIEKTQNRWIEQRGGNPFIEITVPEAGIKLIQAVGRLIRT
                     EQDYGRVTILDNRIKTQRYGQQLLAGLPPFKRIG"
     gene            2215..2829
                     /locus_tag="ACFM8S_001907"
     CDS             2215..2829
                     /locus_tag="ACFM8S_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFC6314925.1"
                     /translation="MNFTRLLNQVLSTVQKKGGTFSDSPLNSFGGGALVAGIASMLLN
                     GKNRKTITKIGSTAALGYLAYRGYQMWQQNKGRATVTQSDFQPAGETEETYSRTVLRT
                     MIAAAASDGMIDEAERRTIEQESGTDPETAAWLAAEYRLPASIEDIAAAVGNDEALAA
                     EAYLAARLVCADLSRKETVFLARLSQALKLDDNLVESLERQLGF"
BASE COUNT          716 a          936 c          737 g          504 t
ORIGIN      
        1 cccacttcta ccgccgtaca aaacgccatg ctcaccgact tagaaaaaaa cgccatccgc
       61 gaccattacc aaaatattgg caaaaacctg cccggtttcc gtccgcgtgc ttcgcagcgg
      121 gaaatgattg cggcggttgc caacgctttt tcgcggacgt tggcgcgcga agaaggcggc
      181 gagccgccca agcgcgaagg cgagagcatc gccgtaatcg aaggaccgac cggcgtgggc
      241 aaatcgcttg cctacctgct ggcgggcggc atcatggcgc aaacacgcgg caagcggctg
      301 attgtgagca gcgcgacggt tgccttgcag gagcagttgg tagaccgcga cctgccgttt
      361 ctggtcgaaa aaagcggttt ggaactgacc ttcgcacttg ccaaagggcg cggccgctat
      421 ctctgcccct acaaactcta tcaactgacg caaagcaatg cccagcaaaa cctgctcggc
      481 tttgaagccc ccgccgtctt gtgggacagc aaacccaagc ccgaagaatt gaagctgctg
      541 cgcgacatcg ccgacgaatt ttccgcccga cggttcaacg gcgaccgcga cacttggccg
      601 gaaaaaatcg atgacgcgat ttggctcaaa gtgaccaacg accgccacgg ctgcctgaaa
      661 accgcctgtc ccaaccgtcc ggaatgtccg ttttacctag cacgcgatgt cttggaaacc
      721 gtcgatgtcg tcgttgccaa ccacgatctt ctgcttgccg acatcagtat gggcggcggc
      781 gtgattctgc ctgcgcccga aaacagtttc tattgcatag acgaagcgca ccacctgccc
      841 aaaaaagccc tcagccgttt tgccgccgaa cattcatgga atattgccgt ttggacgctg
      901 gaaaaactgc cgcagctgac cggcaaaatt gccgcgctta ccgataaagc cgaacttgcc
      961 aacctggccg acgaagccgc cgcatccttg ctcgacagcc tgcacgaatg gcagttccat
     1021 ttggcggaag agccgtcttt aagtatgggg ccgtctgaaa acgacagacg aaccaacagc
     1081 gaaccgactt ggctgtggga agacggcaaa atccccgaag gcctcgaaac caccgtttcc
     1141 aacacagcca ttgccgcacg cagcctgctc aaacacgttg tcgggctgaa cgatgcgctt
     1201 tccgccgcgc gccgcgaaaa agaacaggac ggcgcgctcc tcgaccgcct gaccggcgat
     1261 ttcggcatct tcatcgcccg tatcgaacaa atcagcgcgg tttgggattt gctctctacc
     1321 gttcccattg agggcgaaga gccgttggcg aaatggataa cccgccgcgc cgacgacaaa
     1381 aacgactaca ttttcaacgc cagccccatc agcagcgcgt cccacctcgc caacagcctg
     1441 tggcggcgtg cggcaggcgc ggtgttgacc tccgccaccc tgcaatcctt gggcaacttt
     1501 aacctgatgc tgcgccaaac cgggctgcaa tggctgcccg aaaccaccac cctcgccctc
     1561 aaaagcccct ttgactttga aaaacagggc gaactctaca tcccccccat atacgccagc
     1621 cccaaagacc ccgaagccca caccgccgcc atcatcgaat ggctgcccaa gcttatttcg
     1681 cccatcgaag ccatcggcac actcgtcttg ttttcctcgc gcaaacaaat gcaggatgtc
     1741 gccctgcgcc tgcccggaaa ctacctgccg ctcctgctcg tacaaggcga attgcccaaa
     1801 gccgtcctcc tgcaaaaaca ccaccgggcc atagaagaag gcaaagccag catcatcttc
     1861 ggactcgaca gtttcgccga aggactcgac ctgcccggca ccgcctgcgt gcaagtcatc
     1921 atcgccaagc tgccgtttgc gatgcctgac aaccccatcg aaaaaaccca aaaccgctgg
     1981 atagaacagc gcggcggcaa tccttttatt gaaatcaccg tccccgaagc cggcatcaaa
     2041 ctcatccagg ccgtcggccg cctcatccgc accgaacaag actacggccg cgtaaccatc
     2101 ctcgacaacc gcatcaaaac acagcggtac ggccaacaat tattggccgg cctgccgccg
     2161 tttaaaagga tagggtaaac ataccgccct tcccgcaaca gaaaggaaaa attcatgaac
     2221 tttacccgac tgctcaacca agtcttaagc acggttcaaa aaaaaggcgg cacattctcc
     2281 gacagcccgc tcaattcatt cggcggaggc gcgctggttg ccggtatcgc ctccatgctg
     2341 ctgaacggta aaaaccgcaa aaccattacc aaaatcggtt cgaccgccgc tttgggctac
     2401 ctcgcctacc ggggctatca gatgtggcag caaaacaaag ggcgggcaac tgtaacacaa
     2461 agcgatttcc aacctgccgg agaaactgaa gaaacataca gccgcaccgt attgcgcacc
     2521 atgatagccg ccgccgcttc agacggcatg atagacgaag ccgaacgccg gactatcgaa
     2581 caggaaagcg gcacagaccc cgaaactgcc gcatggctcg ccgccgaata ccgcctgccc
     2641 gcaagcatcg aagacatcgc cgccgccgtc ggcaacgatg aggcgttggc ggcggaggcc
     2701 tatctggcgg caaggttggt ctgtgccgat ttgtcgcgga aagaaaccgt cttcctcgcc
     2761 cgcctgtcgc aggctttgaa actggatgac aatctggtgg agagtttgga aaggcaattg
     2821 gggttttgat gacacagccg gggagggaaa atcatcaaaa aacggttatt ccgaccccgg
     2881 cctcaaaacg gca
//
LOCUS       DAXHSF010000194         2833 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.194, whole genome shotgun sequence.
ACCESSION   DAXHSF010000194 DAXHSF010000000
VERSION     DAXHSF010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.194"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(39..515)
                     /gene="greA"
                     /locus_tag="ACFM8S_001908"
     CDS             complement(39..515)
                     /gene="greA"
                     /locus_tag="ACFM8S_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFC6314926.1"
                     /translation="MQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSE
                     NAEYEAAKERQGFIEGRISELEHKLSVAHIINPTEIHAEGKIVFGTTVTLEDLETEEH
                     VTYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYDIIEVRYI"
     gene            910..2211
                     /gene="aroA"
                     /locus_tag="ACFM8S_001909"
     CDS             910..2211
                     /gene="aroA"
                     /locus_tag="ACFM8S_001909"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFC6314927.1"
                     /translation="MTESVRLPVARLKPSTVALPGSKSISNRTLLLAALSDNACEIHS
                     LLKSDDTDRMLEALDKLGVQIEYLAEDRLKVHGTGGRFPNRTADLFLGNAGTAFRPLT
                     AALAVLGGDYHLHGVPRMHERPIGDLADALRIAGADVEYLGKEHYPPLHIGKRQDNGE
                     HVIPIKGNVSSQFLTALLMALPLTGQAFEIRMVGELISKPYIDITLKLMAQFGVQVAN
                     EDYRVFKIPADAHYHAPEHLHVEGDASSASYFLAAGLIAATPVRVTGIGANSIQGDVA
                     FARELEKIGADVVWGENFVEVSRPKERAVQSFDLDANHIPDAAMTLAIVALATGQTCT
                     LRNIGSWRVKETDRIAAMANELRKLGAKVVEEAEAIHITPPETLTPDAVIDTYDDHRM
                     AMCFSLVSLLGVPVVINDPKCTHKTFPTYFDVFSSLTETAE"
     gene            complement(2254..2787)
                     /locus_tag="ACFM8S_001910"
     CDS             complement(2254..2787)
                     /locus_tag="ACFM8S_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFC6314928.1"
                     /translation="MFPPDKTLFLCLSALLLASCGTTSGKHRQPKPKQTVRQIQAVRI
                     SHIDRTQSSQELMLHSLGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTAR
                     DMAAASRKIPDSRLKAGDLVFFNTGGAHRYSHVGLYIGNGEFIHAPSSGKTIRTEKLS
                     TPFYAKNYLGAHTFFTE"
BASE COUNT          594 a          780 c          773 g          686 t
ORIGIN      
        1 tttcctgcgt gtgtcgggtg tatcgaaatc aagccgaatc aaatatatcg gacttcgata
       61 atgtcgtatt cgcgcacgcc gcccggggct tggacttccg ccgtatcgcc ctcttccttg
      121 ccgattaagg cgcgggcaat cggtgagccg acatagattt tgccctgttt gatgtcggct
      181 tcgtcttcgc cgacaatttg ataggtaacg tgttcttccg tttccaaatc ttccagcgta
      241 accgtcgtac cgaacacgat tttgccttcg gcgtggattt cggtcggatt gatgatgtgg
      301 gcaacggaaa gtttgtgttc cagctcggaa atgcggccct cgataaagcc ttggcgttct
      361 ttggcggctt cgtattcggc gttttcggac aaatcgccgt gcgaacgggc ttcggcaatc
      421 gcttcgatca cttcgggacg cgccacgctt ttgagctgct gcaattcctg tttcagcaat
      481 tccgcaccgc gtacggtcag ggggattttt tgcatcggtc ttttttctcc atattccggc
      541 acaccgtttt gcggcagcaa gcataccgcg taccgtcttg ttttgtgcgt ccggatatta
      601 aaataaaaat acaagccgcc cggaaaatcg gcggcttgtc tgtcgttgaa cagcggctat
      661 tctaccaaat tctatgaaat tggcaatcgt gccgtgccgc cgacaaacgc gccatgtccg
      721 caacaaaagc tgaaaatatg ccgtcaaaga aattttagaa acaaaaaatt taaaaataat
      781 caattttcgg cataaaaaac cacatttacg gactttaaaa ccgaaaatgc caagcctgag
      841 atttttcata cagcatttgc accagtataa tgcaggctgt ttttatcttt ataatattga
      901 cgttttgcca tgaccgaatc cgtccgcctc cccgtcgccc gtctcaaacc ttccaccgtc
      961 gccctgcccg gctccaaaag catcagcaac cgcaccctgc tgcttgccgc cttgtccgac
     1021 aatgcttgcg aaatccattc cctgctcaaa tccgacgata ccgaccgtat gctcgaagca
     1081 ctcgataaac tcggcgttca aatcgaatat cttgccgaag accgtctgaa agtgcacggc
     1141 acaggcggac gcttccccaa ccgcactgcc gatttgtttt tgggcaacgc gggcacggcg
     1201 ttccgtccgc tgactgccgc acttgccgtt ttgggcggcg attatcatct gcacggcgtg
     1261 cctcgtatgc acgaacgccc catcggcgat ttggccgatg cactgcggat tgccggcgcc
     1321 gatgtcgaat atctcggcaa ggaacactat ccgccgcttc atatcggcaa acgtcaagac
     1381 aacggcgaac acgtgattcc gattaaaggc aatgtgtcca gccagtttct gaccgccctt
     1441 ttaatggcgt tgccgctgac cggacaggca tttgaaatcc gtatggtcgg cgagttgatt
     1501 tccaagccct atatcgacat tactttaaaa ctgatggcgc aattcggcgt acaggttgcc
     1561 aatgaagact accgcgtctt caaaattccc gccgatgcgc actaccacgc gcccgaacac
     1621 ttgcacgtcg aaggcgatgc ctccagcgcg tcctacttcc tcgcagccgg tttgattgcc
     1681 gccacgcccg tgcgcgtgac cggcatcggc gcaaacagca tacagggcga tgtcgccttt
     1741 gcccgcgagc tggaaaaaat cggtgcggac gtggtttggg gcgaaaactt cgtcgaagtt
     1801 tcacgcccga aggaacgtgc cgtccaatcc tttgatttgg atgcgaacca tatccccgat
     1861 gccgccatga ccctcgccat cgtcgcgctt gctacagggc aaacctgcac gctgcgcaac
     1921 atcggttcgt ggcgcgtcaa agagaccgac cgcatcgccg caatggcaaa cgagttgcgc
     1981 aaactcgggg caaaagtcgt cgaagaagcc gaagcaattc acatcacccc gcccgaaacg
     2041 ctgacacccg acgccgtcat cgacacgtac gacgaccacc gcatggcgat gtgtttctcg
     2101 ctggtttcgc tgttgggcgt acccgtcgtc atcaacgatc cgaaatgcac ccacaaaacc
     2161 ttcccgactt atttcgacgt gttctcatcg ctgaccgaaa cagcggaata aggcggcatt
     2221 ttgccgcgat tccggcgcgg cgcggcgggc ggctcattct gtaaaaaaag tatgtgcgcc
     2281 gaggtagttt ttagcgtaaa acggtgtaga aagtttttcg gttcggatgg ttttgccgct
     2341 gctgggggca tggatgaatt cgccgttgcc gatgtagagt ccgacgtgtg agtagcggtg
     2401 tgcgccgccg gtgttgaaga atacgaggtc gccggccttg aggcggctgt cggggatttt
     2461 gcggcttgcc gccgccatgt ctcgggcggt gcgcggcagc ttgacgttga gggcgttttt
     2521 gtaaacgaat tggatcatgc cgctgcaatc gaagccggtt gcggtgctgc tgccgcccca
     2581 tttgtagggc gtgccgatga gtccgaggct gtggagcatg agttcctgcg agctttgtgt
     2641 gcggtcgatg tggctgatgc ggacggcttg gatttgccgg actgtctgtt tgggtttcgg
     2701 ttggcggtgt ttgccggagg tcgtgccgca tgaggcgagg agcagtgcgc tgagacagag
     2761 gaaaagggtt ttgtcggggg gaaacatggt ttttcctttg cgggttcgga tatccgtctg
     2821 aaggtgtttc aga
//
LOCUS       DAXHSF010000195         2789 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.195, whole genome shotgun sequence.
ACCESSION   DAXHSF010000195 DAXHSF010000000
VERSION     DAXHSF010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.195"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            117..440
                     /locus_tag="ACFM8S_001911"
     CDS             117..440
                     /locus_tag="ACFM8S_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFC6314929.1"
                     /translation="MQNETINPKQHLAAIKEYWQPEIINRHGFQFRLVKLLGDYGWHT
                     HGYSDKVLFAVEGDMAVDFADGGSMTIREGEMAVVPKSVSHRPRSENGCSLVLIELSD
                     PSEAV"
     gene            554..2731
                     /gene="tdfF"
                     /locus_tag="ACFM8S_001912"
     CDS             554..2731
                     /gene="tdfF"
                     /locus_tag="ACFM8S_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent iron piracy receptor TdfF"
                     /protein_id="HFC6314930.1"
                     /translation="MTRFKYSLLFAALLPVYAQADVSVSDDPKPQESTELPTITVTAD
                     RTASSNDGYTVSGTHTPLGLPMTLREIPQSVSVITSQQMRDQNIKTLDRALLQATGTS
                     RQIYGSDRAGYNYLFAHGSRIANYQINGIPVADALADTGNANTAAYERVEVVRGVAGL
                     LDGTGEPSATVNLVRKRPTRKPLFEVRAEAGNRKHFGLGADVSGSLNTEGTLRGRLVS
                     TFGRGDSWRQRERSRNAELYGILEYDIAPQTRVHAGMDYQQAKETADAPLSYAVYDSQ
                     GYATAFGPKDNPATNWANSRHRALNLFAGIEHRFNQDWKLKAEYDYTRSRFRQPYGVA
                     GVLSIDHNTAATDLIPGYWHADPRTHSASVSLIGKYRLFGREHDLIAGINGYKYASNK
                     YGERSIIPNAIPNAYEFSRTGAYPQPASFAQTIPQYGTRRQIGGYLATRFRAADNLSL
                     ILGGRYSRYRTGSYDSRTQGMTYVSANRFTPYTGIVFDLTGNLSLYGSYSSLFVPQSQ
                     KDEHGSYLKPVTGNNLEAGIKGEWLEGRLNASAAVYRARKNNLATAAGRDPSGNTYYR
                     AANQAKTHGWEIEVGGRITPEWQIQAGYSQSKTRDQDGSRLNPDSVPERSFKLFTAYH
                     FAPEAPSGWTIGAGVRWQSETHTDPATLRIPNPAAKARAADNSRQKAYAVADIMARYR
                     FNPRAELSLNVDNLFNKHYRTQPDRHSYGALRTVNAAFTYRFK"
BASE COUNT          682 a          993 c          696 g          418 t
ORIGIN      
        1 aggcttacat caaagccgat aacgcttccg ttacaactcc gcccactgaa agcagcctgc
       61 aacgaagcca aaacgacaaa ccgcatcgta aaccacccaa cccataggag aaccccatgc
      121 aaaacgaaac catcaacccg aaacagcacc ttgccgccat caaagaatac tggcagcccg
      181 aaatcatcaa ccgccacggg ttccaattcc gcttggtcaa acttttgggc gattacggct
      241 ggcatacgca cggatacagc gacaaagtgc tgtttgccgt ggagggcgac atggcggtgg
      301 acttcgccga cggcggcagc atgacgatac gcgagggcga gatggcggtc gtgccgaagt
      361 cggtgtcgca ccgcccgcgt tcggaaaacg gctgctcgtt ggtgctgatt gagttgtccg
      421 acccgtccga ggccgtctga aaacgaagtt tccgaaggaa gctgagtttc tgcgaagcta
      481 aaagcagcct gcaccttcaa tcaatatgcc gaaaatacaa ccccaccgca caccaacaca
      541 caaaggaaat cccatgacac gcttcaaata ttccctgctg tttgccgccc tgttgcccgt
      601 gtacgcgcag gccgatgttt ctgtttcaga cgaccccaaa ccgcaggaaa gcactgaatt
      661 gccgaccatc accgttaccg ccgaccgcac cgcgagttcc aacgacggct acactgtttc
      721 cggcacgcac accccgctcg ggctgcccat gaccctgcgc gaaatcccgc agagcgtcag
      781 cgtcatcaca tcgcaacaaa tgcgcgacca aaacatcaaa acgctcgacc gcgccctgtt
      841 gcaggcgacc ggcaccagcc gccagattta cggctccgac cgcgcgggct acaactacct
      901 gttcgcgcac ggcagccgca tcgccaacta ccaaatcaac ggcatccccg ttgccgacgc
      961 gctggccgat acgggcaatg ccaacaccgc cgcctatgag cgcgtagaag tcgtgcgcgg
     1021 cgtggcgggg ctgctggacg gcacgggcga gccttccgcc accgtcaatc tggtgcgcaa
     1081 acgcccgacc cgcaagccat tgtttgaagt ccgcgccgaa gcgggcaacc gcaaacattt
     1141 cgggctgggc gcggacgtat cgggcagcct gaacaccgaa ggcacgctgc gcggccgcct
     1201 ggtttccacc ttcggacgcg gcgactcgtg gcggcagcgc gaacgtagcc gcaatgccga
     1261 actctacggc attttggaat acgacatcgc accgcaaacc cgcgtccacg caggcatgga
     1321 ctaccagcag gcgaaagaaa ccgccgacgc gccgctcagc tacgccgtgt acgacagcca
     1381 aggttatgcc accgccttcg gcccgaaaga caaccccgcc acaaattggg cgaacagccg
     1441 ccaccgtgcg ctcaacctgt ttgccggcat cgaacaccgc ttcaaccaag actggaaact
     1501 caaagccgaa tacgactaca cccgcagccg cttccgccag ccctacggcg tagcaggcgt
     1561 gctttccatc gaccacaaca ccgccgccac cgacctgatt cccggttatt ggcacgccga
     1621 cccgcgcacc cacagcgcca gcgtgtcatt gatcggcaaa taccgcctgt tcggccgcga
     1681 acacgattta atcgcgggta tcaacggtta caaatacgcc agcaacaaat acggcgaacg
     1741 cagcatcatc cccaacgcca ttcccaacgc ctacgaattt tcccgcacgg gtgcctaccc
     1801 gcagcctgca tcgtttgccc aaaccatccc gcaatacggc accaggcggc aaatcggcgg
     1861 ctatctcgcc acccgtttcc gcgccgccga caacctttcg ctgatactcg gcggcagata
     1921 cagccgttac cgcaccggca gctacgacag ccgcacacaa ggcatgacct atgtgtccgc
     1981 caaccgtttc accccctaca caggcatcgt gttcgacctg accggcaacc tgtctcttta
     2041 cggctcgtac agcagcctgt tcgtcccgca atcgcaaaaa gacgaacacg gcagctacct
     2101 gaaacccgta accggcaaca atctggaagc cggcatcaaa ggcgaatggc ttgaaggccg
     2161 tctgaacgca tccgccgccg tgtaccgcgc ccgtaaaaac aacctcgcca ccgcagcagg
     2221 acgcgacccg agcggcaaca cctactaccg cgccgccaac caagccaaaa cccacggctg
     2281 ggaaatcgaa gtcggcggcc gcatcacgcc cgaatggcag atacaggcag gttacagcca
     2341 aagcaaaacc cgcgaccaag acggcagccg cctgaacccc gacagcgtac ccgaacgcag
     2401 cttcaaactc ttcactgcct accactttgc ccccgaagcc cccagcggct ggaccatcgg
     2461 cgcaggcgtg cgctggcaga gcgaaaccca caccgaccct gccacgctcc gcatccccaa
     2521 ccccgccgcc aaagcccgcg ccgccgacaa cagccgccaa aaagcctacg ccgtcgccga
     2581 catcatggcg cgttaccgct tcaatccgcg cgccgaactg tcgctgaacg tggacaatct
     2641 gttcaacaaa cactaccgca cccagcccga ccgccacagc tacggcgcac tgcggacagt
     2701 gaacgcggcg tttacctatc ggtttaaata aggtcgtctg aaaacggagt ttctgcgaag
     2761 ctatagtgga ttaacaaaaa ccagtacgg
//
LOCUS       DAXHSF010000196         2751 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.196, whole genome shotgun sequence.
ACCESSION   DAXHSF010000196 DAXHSF010000000
VERSION     DAXHSF010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.196"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..313
                     /locus_tag="ACFM8S_001913"
     CDS             <1..313
                     /locus_tag="ACFM8S_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC6314931.1"
                     /translation="SEIWSNPKATFLDPVCKSGVFLREIVKRLDEGLTNQIPDKQTRI
                     NHILKNQVFGSTATYVGSFDRYLHKNSFAGERNYFANFKIFLAPNSTMTKTIMPMIKS
                     G"
     gene            complement(232..1032)
                     /locus_tag="ACFM8S_001914"
     CDS             complement(232..1032)
                     /locus_tag="ACFM8S_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HFC6314932.1"
                     /translation="MKKTIFNITKMDCPSEEQLIRMRLKDVSDIYELQFDIAGRCLTV
                     YHDNQDTTILQVLEPLNFDSHIISTEVIVDKIVFNKPDEHLEKRLLYQVLMINFVFFI
                     IECSVGIFANSMGLIADSLDMLADSFVYILALSAIGMTLAYKKRVAFLAGITQIILAL
                     FGVIEVIRRFIGAEQLPNYQLMIGTAFLALIANWLCLYLLSKNQNKEIHIKASMIFTS
                     NDIIINIGVIAAGALTLLTHSSYPDLIIGMIVFVIVLFGARNILKLAK"
     gene            complement(1168..1674)
                     /locus_tag="ACFM8S_001915"
     CDS             complement(1168..1674)
                     /locus_tag="ACFM8S_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314933.1"
                     /translation="MDFFDFFAQGKYKKIATEQGLDRKYFIFHYIVCNLSAISSFCLM
                     KVIHSYTILCLLIMIIGIAFGFILRNRVLSIVKNKQNFLSDMFPLEMPFFSRVYKIPI
                     LARFLNRGMKWLYLQNFFCLVMICFSISLILNEERGWMPYFICYWFGLCYPANTYYVF
                     LSRDYKHI"
     gene            complement(2011..2652)
                     /locus_tag="ACFM8S_001916"
     CDS             complement(2011..2652)
                     /locus_tag="ACFM8S_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GrxB family glutaredoxin"
                     /protein_id="HFC6314934.1"
                     /translation="MKLYIYDHCPFCVRARMAAGLFGADVEEAVLANDDEATPIGMIG
                     AKQVPVLQKEDGSFMGESLDIVRHFDREDRLKDEVRPEIRAWLDKVGGYNDKLVQPRV
                     IKIGLPEFATPEAVKYFTDKKEKSIGSFSANLNKTAQYLERINADLQELENLMDGASD
                     GINGGIGMEDILVFPVLRNLTVVRGIEWPQKVMGYLMTMSEKSGVPLYFDRAL"
BASE COUNT          887 a          588 c          475 g          801 t
ORIGIN      
        1 gtctgagatt tggtcaaatc caaaggcgac attcttagac cctgtgtgta aatcaggggt
       61 atttttgcgt gaaatcgtca aacgcttgga tgaaggcttg accaatcaaa taccagataa
      121 acaaactcgc attaaccaca ttttaaaaaa tcaagttttt ggaagtactg ccacgtatgt
      181 aggtagcttt gaccgatatt tgcataaaaa ctcctttgct ggtgaaagga attattttgc
      241 caattttaaa atatttctgg caccaaatag tacaatgaca aagacaatca tgccaatgat
      301 taaatcagga tagctagaat gagtcaataa cgtcaatgct cccgccgcta tcacaccgat
      361 attgatgata atgtcattgg atgtaaaaat catgctggct ttgatatgga tttctttatt
      421 ttgatttttg ctcagtagat ataagcacag ccagtttgca atcaatgcca aaaatgccgt
      481 gccaatcatc agttgataat tgggcagctg ctcagcaccg ataaaacgcc taatcacttc
      541 tatcacccca aataacgcca atattatctg cgttatcccc gccaaaaatg ccacacgttt
      601 tttatacgcc agcgtcatac caatggctga tagcgccaat atatagacaa agctgtccgc
      661 cagcatatct agactatcag caatcagccc catagaatta gcaaaaatac caaccgaaca
      721 ctctatgata aaaaacacaa agttaatcat gagcacttga tataataatc ttttttctaa
      781 gtgctcatca ggcttgttaa acactatctt atcaacaatc acttcggtgg aaatgatatg
      841 actatcaaaa ttaagcggtt caagtacttg taaaatcgtt gtatcttgat tatcgtgata
      901 gacggttaag caccgcccag caatatcaaa ctgtaattca taaatatcag acacatcttt
      961 taaacgcatg cgaatgagct gttcttcgga cgggcagtcc attttggtaa tgttaaaaat
     1021 ggtctttttc atctatttag ttccttgttt tgatcaggtt ggctcaaata aatctgtgtt
     1081 tatattgctg cttggtaatt tttggatggt ttgagtaaat tgattaggtt aaaatttacc
     1141 tttggaagta ccgccacgca taatagttta gatatgttta taatctctgg ataaaaaaac
     1201 gtaataagtg tttgctggat aacaaagtcc aaaccaatag cagataaaat aaggcatcca
     1261 ccccctttct tcattaagga taagagatat cgagaaacaa atcataacta aacagaaaaa
     1321 attttggaga taaagccatt tcattcccct attcaagaat ctggccaaga taggtatttt
     1381 gtatactcta gaaaagaaag gcatttccaa gggaaacata tcagataaaa aattttgttt
     1441 attttttact atagatagaa ccctatttct caagataaat ccaaatgcaa tgccaattat
     1501 cattattaat aaacaaagga tagtataact atggataacc ttcattaaac aaaaagaaga
     1561 aatggcagat aaattacata cgatatagtg gaatataaaa tatttacggt ctaaaccttg
     1621 ttcagttgca atttttttat acttaccttg cgcaaaaaaa tcaaagaaat ccattaaact
     1681 atctttcaca ttagaaattt aaaagctaaa taatacgaca aacaatgtga agtactattc
     1741 atggtttatt taaaaaataa tactattctg aacattattt agatacagaa attaacaaat
     1801 tagaactaaa caagctttta aatactttaa ttttattgga aagctataaa aggaactata
     1861 actttacaca ctagtcactt ctttttaaga ggcaaaaggg attgggaagg tcgtcttgga
     1921 gataagcact ggtatttcgg ccaatggtaa atagagttta cctcaaatag ggtagaacct
     1981 ccttcatctg tcagttaata acagccactt ttacaatgcc ctgtcaaaat aaagcggcac
     2041 gcccgatttt tcactcatcg tcatcaaata acccatcacc ttttggggcc attcgatgcc
     2101 gcgcaccacg gtcagattcc tcaaaacggg gaaaaccaaa atatcctcca taccgattcc
     2161 gccgttgatg ccgtctgaag caccgtccat caaattttcc aactcttgca aatctgcgtt
     2221 tatccgttcg aggtattggg cggttttatt caaattggcg gaaaagctgc cgatgctttt
     2281 ctcttttttg tctgtaaaat atttcaccgc ttccggcgtt gcaaattcag gcagcccgat
     2341 tttgatcacg cgcggctgca ccagtttgtc gttgtatccg cccaccttgt ccagccacgc
     2401 ccgtatctcg gggcggactt cgtctttcag acggtcttcg cggtcgaaat gccgcacaat
     2461 gtccaaactc tcgcccataa acgaaccgtc ttctttttgc aggacgggca cttgtttcgc
     2521 accgatcata ccgatcggcg ttgcctcgtc gtcgtttgcc agcacggctt cttcaacgtc
     2581 cgcgccaaac agcccggcag ccatccgcgc acgcacgcaa aacgggcaat ggtcgtaaat
     2641 atacagtttc atcaaaatat tcctcgtcaa cctgtcggta ccggctacct taacaccccg
     2701 cgccacccga aacaagttta tcttcccgcc tatgcaccgt aaataaataa g
//
LOCUS       DAXHSF010000197         2600 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.197, whole genome shotgun sequence.
ACCESSION   DAXHSF010000197 DAXHSF010000000
VERSION     DAXHSF010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.197"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            580..2379
                     /gene="nhhA"
                     /locus_tag="ACFM8S_001917"
     CDS             580..2379
                     /gene="nhhA"
                     /locus_tag="ACFM8S_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin NhhA"
                     /protein_id="HFC6314935.1"
                     /translation="MNKIYRIIWNSALNAWVVVSELTRNHTKRASATVETAVLATLLF
                     ATVQASANNEEQEEDLDPVQRTVAVLIVNSDKEGTGEKEKVEENSDWAVYFNEKGVLT
                     AGTITLKAGDNLKIKQNTNENTNENTNASSFTYSLKKDLTDLTSVGTEKLSFSANGNK
                     VNITSDTKGLNFAKETAGTNGDTTVHLNGIGSTLTDTLLNTGATTNVTNDNVTDDEKK
                     RAASVKDVLNAGWNIKGVKPGTTASDNVDFVRTYDTVEFLSADTKTTTVNVESKDNGK
                     RTEVKIGAKTSVIKEKDGKLVTGKGKGENGSSTDEGEGLVTAKEVIDAVNKAGWRMKT
                     TTANGQTGQADKFETVTSGTNVTFASGKGTTATVSKDDQGNITVKYDVNVGDALNVNQ
                     LQNSGWNLDSKAVAGSSGKVISGNVSPSKGKMDETVNINAGNNIEITRNGKNIDIATS
                     MTPQFSSVSLGAGADAPTLSVDGDALNVGSKKDNKPVRITNVAPGVKEGDVTNVAQLK
                     GVAQNLNNRIDNVDGNARAGIAQAIATAGLVQAYLPGKSMMAIGGGTYRGEAGYAIGY
                     SSISDAGNWVIKGTASGNSRGHFGASASVGYQW"
BASE COUNT          822 a          651 c          624 g          503 t
ORIGIN      
        1 gtcattcccg cgcaggcggg aatccagcaa cacaacgcgg caggacttta tcggaaaaaa
       61 cagaaacccc accgaccgtc attcccgcaa aagcgggaat ccagacccgt cggcacggaa
      121 acttaccgga taaaacagtt tccttagatt ccacgtccta gattcccgcc ttcgcgggaa
      181 tgacgagatt ttagattatg ggaatttatc aggaatgatt gaatccatag aaaaatcaca
      241 ggaatctatc agaaaaaaca gaaaccccac cgaccgtcat tcccgcaaaa gcgggaatct
      301 agaaacacaa cgcggcagga ctttatcaga aaaaacagaa accccaccgc cgtcattccc
      361 gcaaaagcgg gaatccagac ccgtcggcac ggaaacttac cggataaaac agtttcctta
      421 gattccacgt cctagattcc cgctttcgcg ggaatgacga gattttaagt tgggggaatt
      481 tatcagaaaa cccccaaccc ccaaaaaccg ggcggatgcc gcaccatccg cccccaaacc
      541 ccgatttaac cattcaaaca aaccaaaaga aaaaacaaaa tgaacaaaat ataccgcatc
      601 atttggaata gtgccctcaa tgcctgggtc gtcgtatccg agctcacacg caaccacacc
      661 aaacgcgcct ccgcaaccgt ggagaccgcc gtattggcga cactgttgtt tgcaacggtt
      721 caggcaagtg ctaacaatga agagcaagaa gaagatttag accccgtaca acgcactgtt
      781 gccgtgttga tagtcaattc cgataaagaa ggcacgggag aaaaagaaaa agtagaagaa
      841 aattcagatt gggcagtata tttcaacgag aaaggagtac taacagccgg aacaatcacc
      901 ctcaaagccg gcgacaacct gaaaatcaaa caaaacacca atgaaaacac caatgaaaac
      961 accaatgcca gtagcttcac ctactcgctg aaaaaagacc tcacagatct gaccagtgtt
     1021 ggaactgaaa aattatcgtt tagcgcaaac ggcaataaag tcaacatcac aagcgacacc
     1081 aaaggcttga attttgcgaa agaaacggct gggacgaacg gcgacaccac ggttcatctg
     1141 aacggtattg gttcgacttt gaccgatacg ctgctgaata ccggagcgac cacaaacgta
     1201 accaacgaca acgttaccga tgacgagaaa aaacgtgcgg caagcgttaa agacgtatta
     1261 aacgcaggct ggaacattaa aggcgttaaa cccggtacaa cagcttccga taacgttgat
     1321 ttcgtccgca cttacgacac agtcgagttc ttgagcgcag atacgaaaac aacgactgtt
     1381 aatgtggaaa gcaaagacaa cggcaagaga accgaagtta aaatcggtgc gaagacttct
     1441 gttatcaaag aaaaagacgg taaattggtt actggtaaag gcaaaggcga gaatggttct
     1501 tctacagacg aaggcgaagg cttagtgact gcaaaagaag tgattgatgc agtaaacaag
     1561 gctggttgga gaatgaaaac aacaaccgct aatggtcaaa caggtcaagc tgacaagttt
     1621 gaaaccgtta catcaggcac aaatgtaacc tttgctagtg gtaaaggtac aactgcgact
     1681 gtaagtaaag atgatcaagg caacatcact gttaagtatg atgtaaatgt cggcgatgcc
     1741 ctaaacgtca atcagctgca aaacagcggt tggaatttgg attccaaagc ggttgcaggt
     1801 tcttcgggca aagtcatcag cggcaatgtt tcgccgagca agggaaagat ggatgaaacc
     1861 gtcaacatta atgccggcaa caacatcgag attacccgca acggtaaaaa tatcgacatc
     1921 gccacttcga tgaccccgca gttttccagc gtttcgctcg gcgcgggggc ggatgcgccc
     1981 actttgagcg tggatgggga cgcattgaat gtcggcagca agaaggacaa caaacccgtc
     2041 cgcattacca atgtcgcccc gggcgttaaa gagggggatg ttacaaacgt cgcacaactt
     2101 aaaggcgtgg cgcaaaactt gaacaaccgc atcgacaatg tggacggcaa cgcgcgtgcg
     2161 ggcatcgccc aagcgattgc aaccgcaggt ctggttcagg cgtatctgcc cggcaagagt
     2221 atgatggcga tcggcggcgg cacttatcgc ggcgaagctg gttacgccat cggctactcg
     2281 agtatttccg acgctgggaa ttgggttatc aagggcacgg cttccggcaa ttcgcgcggc
     2341 catttcggtg cttccgcatc tgtcggttat cagtggtaag ggctttttcg cctgtctgct
     2401 gttgggacag gcggaaggtt tgaagggaag ggtggcgatt tgccgcccga gacctttgca
     2461 aaatccccca aaatccccca aaatccccta aattcccacc aagacattta ggagatttct
     2521 catgagcatc ttctttcagc aaaccgccca agccatgatt gccaaacaca tcgaccgttt
     2581 cccgctattg aagttggacc
//
LOCUS       DAXHSF010000198         2598 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.198, whole genome shotgun sequence.
ACCESSION   DAXHSF010000198 DAXHSF010000000
VERSION     DAXHSF010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.198"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(214..285)
                     /locus_tag="ACFM8S_001918"
     tRNA            complement(214..285)
                     /locus_tag="ACFM8S_001918"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(250..252),aa:Arg,seq:cct)
     gene            complement(305..751)
                     /locus_tag="ACFM8S_001919"
     CDS             complement(305..751)
                     /locus_tag="ACFM8S_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFC6314936.1"
                     /translation="MNFSFVPLFLVTLILLGVVSNNNSITISATILLLMQQTALIQFV
                     PLVEKHGLNLGIILLTIGVLSPLVSGKAQVPPVAEFLNFKMISAVFIGIFVAWLAGRG
                     VPLMGQQPVLITGLLIGTVIGVAFMGGIPVGPLIAAGILSFVVGKG"
     gene            complement(756..1730)
                     /gene="ispB"
                     /locus_tag="ACFM8S_001920"
     CDS             complement(756..1730)
                     /gene="ispB"
                     /locus_tag="ACFM8S_001920"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFC6314937.1"
                     /translation="MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISA
                     GGKRLRPIMTILAGKAVGYDDEKLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRATAN
                     NLFGNAAAVLVGDFLYTRAFQLMVASGSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
                     TEEQYIQVIQYKTAKLFEAAAQVGAILGKASPEHERALKDYGMYVGTAFQIIDDVLDY
                     SGETEETGKNVGDDLAEGKPTLPLIYLMRQGSEQVANDVRTALENADRSYFEKIHDYV
                     VRSDALAYSIGEARKAVDCAVTALDALPDSEVKDAMIQLAKESLVRVS"
     gene            1954..2262
                     /gene="rplU"
                     /locus_tag="ACFM8S_001921"
     CDS             1954..2262
                     /gene="rplU"
                     /locus_tag="ACFM8S_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFC6314938.1"
                     /translation="MYAVVKTGGKQYKVSVGEKLKVEQIPAELDSQIELTEVLMIADG
                     ESVKVGAPFIEGAKVTAKVVAHGRGEKVRIFKMRRRKHYQKRQGHRQNFTQIEIVAIA
                     "
     gene            2287..2559
                     /gene="rpmA"
                     /locus_tag="ACFM8S_001922"
     CDS             2287..2559
                     /gene="rpmA"
                     /locus_tag="ACFM8S_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFC6314939.1"
                     /translation="MASKKAGGSTRNGRDSEAKRLGVKAYGNELIPAGSIIVRQRGTK
                     FHAGDNVGMGKDHTLFAKVDGYVEFKTKGALNRKTVSIRPYTGSEE"
BASE COUNT          764 a          697 c          573 g          564 t
ORIGIN      
        1 aatttaatcc actatatcat aaaacaacag atttccaagc cggaacatct tttacggagc
       61 ctgaaaatca tttcatgatt ctaccgtcct aaaggtcggt ttttcaagca ggaagaataa
      121 agttttcaga tggcaaaaaa accctccaac actgaaaggc cttatatcgg aaacttcccg
      181 caacacggga aacagacaaa tgaaatcgtc aaacctcgcc aacaggaatc gaacctgtat
      241 tttacgctta ggaggcatac gttctatccg ttgaactatg gcgagccgaa atgaaaagga
      301 gattttaacc ctttccgacg acaaaagaca agatgccggc cgcaatcagc ggcccgacag
      361 ggataccgcc cataaatgcc accccgataa ccgtcccgat taacagccct gtaattaaaa
      421 caggctgctg tcccatcaaa ggcacgccgc gtcccgccag ccaagccacg aaaataccga
      481 taaaaacggc ggatatcatt ttaaaattca aaaattcggc aacgggagga acctgcgcct
      541 ttcctgaaac caacggactc aaaaccccta tggtcaaaag aatgataccg agattcaacc
      601 cgtgcttctc gaccaacggg acaaactgta tcaatgccgt ctgctgcatc agcagcaata
      661 tggttgccga gatggtaatc gaattgttgt tgctgaccac ccccaacaga atcagcgtaa
      721 ccagaaacag gggaacaaaa ctgaaattca tcgcctcaag acaccctgac caaagattcc
      781 ttcgccagct gaatcatggc atccttcact tcgctgtcgg gcagggcatc caaggcggta
      841 acggcacaat cgactgcttt gcgcgcctcg cctatcgaat atgccaacgc atccgaacgg
      901 acgacataat cgtggatttt ctcaaaatag ctgcgatctg cattttccaa agcagtacgc
      961 acatcgttcg caacctgttc ggaaccctga cgcatcagat aaatcaaagg caaagtcggt
     1021 tttccttccg ccaaatcgtc gccgacgttt ttgccggttt cttcggtttc gccagaatag
     1081 tccagcacat cgtcaataat ttggaatgcc gtaccgacat acataccgta gtctttcaac
     1141 gcccgttcgt gttctgggga agccttgccc aaaattgcgc cgacttgagc ggcagcttca
     1201 aacaattttg ccgttttata ttggatgact tggatatatt gttcttcggt aatgtccgta
     1261 ttgccgatgt tcatcagctg catgacttcg ccctcggcaa taatgttggt tgcatccgcc
     1321 atcacttcca aaacgcgcat actgcccgag gcaaccatca gttgaaaggc gcgcgtgtat
     1381 aaaaagtcgc caaccaacac agccgccgca ttgccgaaca gattgtttgc cgttgcccgc
     1441 ccacggcgca aatcgctttc atcgacgaca tcgtcgtgca ggagggtgga agtgtggata
     1501 aactcgacca tcgccgccag cgaatacagt ttctcgtcat cataaccgac cgccttaccc
     1561 gccaaaatcg tcataatcgg acgcaggcgt ttgccgcccg cgctgatgat atatgtaccg
     1621 atttgcgaaa tcagtgcgac atcggattgc accgcacggt tgatgacttc attgactttg
     1681 gcaaggtctt caggcagatg tcgctggaaa tagggcagat tctcgagcat ggcagactct
     1741 caggtttacg tttccgtcaa acggcagata ccgcttgaaa gctaattcaa atagcgggca
     1801 attatatcag caaagccatc agggtacatc ttttcagacg gcatatggtt atccgaaccg
     1861 cgcaaacttt gacaaaacag gcaaataaca atacaatgcc gagttccgca caatggtgtg
     1921 cggatattaa ccataaccct tatggagttg agtatgtacg cggtcgtaaa aaccggcggc
     1981 aaacagtata aagtttccgt cggcgaaaaa ttgaaagtag aacagatacc agccgaactc
     2041 gacagccaaa tcgaactgac cgaagttttg atgattgctg acggcgaatc tgtaaaagtt
     2101 ggcgcaccct ttatcgaagg tgcaaaagta acggctaaag tagtggcaca cggtcgtggc
     2161 gaaaaagtcc gcatcttcaa aatgcgccgc cgcaaacact accaaaaacg ccaaggccac
     2221 cgccaaaatt tcacccaaat cgaaatcgtg gcaatcgcct aaccttaaaa cttaggagca
     2281 ttacaaatgg caagtaaaaa agcaggcggc agcacccgca acggtcgcga ttcagaagcc
     2341 aaacgcttgg gcgttaaagc ctacggcaac gagctgattc ccgcaggttc catcatcgta
     2401 cgccaacgcg gtaccaaatt ccacgcaggc gacaacgtag gtatgggcaa agaccacact
     2461 ttgttcgcca aagttgacgg ttatgtcgaa ttcaaaacca aaggcgcgct gaaccgtaaa
     2521 actgtcagca tccgtcctta caccggttct gaagaataat ccaaccgata cctgaagccg
     2581 catcttttca cgatgcgg
//
LOCUS       DAXHSF010000199         2556 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.199, whole genome shotgun sequence.
ACCESSION   DAXHSF010000199 DAXHSF010000000
VERSION     DAXHSF010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.199"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..411
                     /locus_tag="ACFM8S_001923"
     CDS             <1..411
                     /locus_tag="ACFM8S_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX iron-regulated FrpC family protein"
                     /protein_id="HFC6314940.1"
                     /translation="GLDSVDDIVIRKDACSLSTTMGERLLTYGVKKMPSAYPEYEAYE
                     DKRHIPENPYFHEFYYIKKGENPAIITHRNNRINQTEEDNYSTGVGSCINGFTVRYYP
                     FIREKQQLTQQELVGYHQQVEQLVQSFVNNPSKK"
     gene            428..703
                     /locus_tag="ACFM8S_001924"
     CDS             428..703
                     /locus_tag="ACFM8S_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314941.1"
                     /translation="MNEGEVVLTPEQIQTLRGYAFRGDTYGGWRYLANLGDCYADDAA
                     AIVGKDTNLNGLNLWMKKGVENLWDDTVGKKIRLMCISVFWIVVFNL"
     gene            complement(790..1923)
                     /locus_tag="ACFM8S_001925"
     CDS             complement(790..1923)
                     /locus_tag="ACFM8S_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314942.1"
                     /translation="MFKKFKPVLLSFFALVFAFWLGTGIAYEINPRWFLSDTATEVPE
                     NPNAFVAKLARLFRNADRAVVIVKESMRTEESLAGAVDDGPLQSEKDYLALAVRLSRL
                     KEKAKWFHVTEQEHGEEVWLDYHIGEGGLVAVSLLQRSPEAFVNAEYLYRNDRPFSVN
                     VYGGAAHGENYETTGEYRVVWQPDGSVFDAAGRGKIGEDVYEHCLGCYQMAQVYLAKY
                     RDVANDEQKVWDFREESNRIASDSRNSVFYQNMRELMPRGMKANSLVVGYDADGLPQK
                     VYWSFDNGKKRQSFEYYLKNGNLFIAQSSTVALKADGVTADMQTYHAQQTWYLDGGRI
                     VREEKQGDRLPDFPLNLEDLEKEVSRYAEAAARRSGGRRDLSH"
     gene            complement(2088..2537)
                     /locus_tag="ACFM8S_001926"
     CDS             complement(2088..2537)
                     /locus_tag="ACFM8S_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314943.1"
                     /translation="MENRFEKIVKDIFSFTQFVSEAENEIPNQSSRYYDCWWELEILN
                     ASALEDWEISGKPEKWEKWTEKYQTEALQSVKHLFAEEVPENNIVCNISHFLHFADRA
                     EADIPNQSRLYAEYWWEMEMFKMSVLEDWERSGRPLNWEKWQRQYRS"
BASE COUNT          706 a          612 c          497 g          741 t
ORIGIN      
        1 ggtttggata gcgtggacga tattgttatc cgaaaagatg cgtgtagttt aagtacgact
       61 atgggagaaa gattgcttac ttacggggtt aaaaaaatgc catctgccta tcctgaatat
      121 gaagcttatg aagataaaag acatattcct gaaaatccat attttcatga attttactat
      181 attaaaaaag gagaaaatcc ggcgattatt actcatcgga ataatcgaat aaaccaaact
      241 gaagaagata attacagcac tggcgtaggt tcctgtatta acggtttcac ggtacggtat
      301 tacccgttta ttcgggaaaa gcagcagctc acacagcagg agttggtagg ttatcaccaa
      361 caagtagagc aattggtaca gagttttgta aacaatccaa gtaaaaaata atttaaagga
      421 tcttattatg aatgagggtg aagttgtttt aacaccagaa caaatccaaa ccttgcgtgg
      481 ttatgctttc cgtggcgata cctatggcgg ttggcgttat ttggctaatt tgggtgactg
      541 ttatgcggat gatgctgctg caattgtcgg taaggataca aacttaaatg gtttgaattt
      601 atggatgaaa aaaggtgtgg aaaacctatg ggatgatacg gtcggtaaaa agatccgttt
      661 aatgtgtatt tccgtttttt ggattgtggt tttcaatttg tagcgaatcg gattcggcat
      721 atacggcatt gcaaaaagcg tttgactctc caatgccgtc tgaaaaccgg tttcagacgg
      781 catttgcgtt cagtgagaaa ggtcgcgcct gccgcccgaa cgtctcgccg cagcctctgc
      841 ataacggctc acctcttttt ccaaatcttc caagttcaaa ggaaaatcag gcagtctgtc
      901 tccctgtttc tcttcgcgga caatccgccc gccatccaaa taccacgtct gttgcgcatg
      961 ataggtctgc atatccgccg ttacgccatc cgctttcaat gctaccgtcg aagattgtgc
     1021 aataaaaaga tttccgtttt tcaaataata ttcgaaactc tggcgttttt tcccattgtc
     1081 gaaactccaa tagactttct gcggcagacc gtccgcatca tagccgacca caagactgtt
     1141 tgccttcatc cctcggggca tcaattcccg catattctga taaaacacag aattgcgcga
     1201 gtccgacgca atccggttgc tctcttcgcg gaagtcccaa accttctgct cgtcattcgc
     1261 gacatcccgg tatttcgcca aatatacctg ggccatctga taacacccga ggcaatgctc
     1321 ataaacatct tccccgattt tcccgcgccc tgccgcatca aataccgaac cgtccggttg
     1381 ccaaacaacc cgatattctc ctgtcgtttc ataattttcc ccgtgagccg ctccgccgta
     1441 cacatttaca gaaaacggac gatcgttccg atacagatat tcggcattaa caaacgcttc
     1501 cggcgagcgt tgcaaaagcg aaaccgcaac caaaccgccc tcgccgatat ggtaatccag
     1561 ccaaacctct tccccatgtt cctgctccgt tacgtgaaac catttcgcct tttctttcaa
     1621 acgactgagc cggacagcga gcgcgagata atccttctcc gactgcaacg gaccgtcatc
     1681 cacagctccg gcaagacttt cctccgtcct catcgattcc ttcacgatga caaccgccct
     1741 gtcggcattt cggaacaggc gggcaagttt cgccacaaaa gcattcggat tttcaggtac
     1801 ttcagttgcc gtatcgctca aaaaccaacg cggattaatc tcataggcaa tacccgttcc
     1861 cagccaaaag gcaaatacaa gtgcaaaaaa tgacaacagt accggtttga attttttaaa
     1921 catatttatt tttcgtttaa cagaatatat cgattatatc agacgagctt tgattgtttg
     1981 atttttaatt taatctacgg ttgctgccgt actttaaaaa cccttatgca ggctgcggct
     2041 tgttactctt gtttgctttg attcaaattt tcaactaatt caaatgctta agatcgatat
     2101 tgcctttgcc atttttccca atttaacggt cttcccgagc gttcccaatc ttctaaaacc
     2161 gacattttga acatttccat ttcccaccaa tattcggcat ataaacgtga ttgattggga
     2221 atgtcggctt ctgcccgatc ggcaaaatgc aaaaaatgag aaatattgca aactatatta
     2281 ttttcaggaa cttcttcggc aaatagatgc ttgactgact gtaacgcttc ggtctgatat
     2341 ttttctgtcc atttctccca tttttcaggc tttcccgata tttcccaatc ttccaatgcg
     2401 gaagcattca agatttccaa ttcccaccaa cagtcgtaat aacgggagga ttggttagga
     2461 atctcatttt ctgcttcgga aacaaattgg gtaaaactaa aaatatcctt aacgattttt
     2521 tcaaatctat tttccataaa ttgctgcctc ttccaa
//
LOCUS       DAXHSF010000200         2512 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.200, whole genome shotgun sequence.
ACCESSION   DAXHSF010000200 DAXHSF010000000
VERSION     DAXHSF010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.200"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(<1..188)
                     /locus_tag="ACFM8S_001927"
     rRNA            complement(<1..188)
                     /locus_tag="ACFM8S_001927"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(595..670)
                     /locus_tag="ACFM8S_001928"
     tRNA            complement(595..670)
                     /locus_tag="ACFM8S_001928"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(635..637),aa:Ala,seq:tgc)
     gene            complement(676..752)
                     /locus_tag="ACFM8S_001929"
     tRNA            complement(676..752)
                     /locus_tag="ACFM8S_001929"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(716..718),aa:Ile,seq:gat)
     gene            complement(853..2393)
                     /locus_tag="ACFM8S_001930"
     rRNA            complement(853..2393)
                     /locus_tag="ACFM8S_001930"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          543 a          723 c          531 g          715 t
ORIGIN      
        1 tccgggttcg cttctctaag tctatgtatt caacttagga tactgcacag aatgcagtgg
       61 gtttccccat tcggacatcg cggaatcatt gctttattgc cagctccccc gcgcttttcg
      121 caggcttaca cgtccttcgt cgcctatcat cgccaaggca tccacctgat gcacttattc
      181 acttgactct atcatttcaa gaacttcttt gactttgcct aacattccgt tgactagaac
      241 atcagacttg aatttcctac tttgataaag cttactgctt tgttgtgtct taatcctgcc
      301 ttttgtgttt caggattaag tcgatacaat catcacccaa atactgtgtt tgttttcttt
      361 tctcttgtga gcgatttttt atcctttgca aagaataaaa aatcaaaaca aacgctttgt
      421 ctttgtttgt tgatttcggc tttccaattt gttaaagatc gatgcgttcg atattgctat
      481 ctactgtgca aatcaaaacg agctgattat tatatcagca ttttgttctt ggtcaagtgt
      541 gacgtcgccc tgaatggatt ctgttccatt cttccgtttt gatttgtaca gtattggtgg
      601 aggcaaacgg gatcgaaccg atgaccccct gcttgcaaag caggtgctct accaactgag
      661 ctatgccccc gttcttggtg ggtctgggag gacttgaacc tccgacccca cgcttatcaa
      721 gcgtgtgctc taaccagctg agctacaaac ccggattctc ttcttaagcg aatcttgcct
      781 tcactcaagc ttcttccgca tcttttcagt ttaccgataa gtgtgaatgc ctaaagcctc
      841 ttctttctct agaaaggagg tgatccagcc gcaggttccc ctacggctac cttgttacga
      901 cttcacccca gtcatgaagc ataccgtggt aagcggactc cttgcggtta ccctacctac
      961 ttctggtatc ccccactccc atggtgtgac gggcggtgtg tacaagaccc gggaacgtat
     1021 tcaccgcagt atgctgacct gcgattacta gcgattccga cttcatgcac tcgagttgca
     1081 gagtgcaatc cggactacga tcggttttgt gagattggct ccgcctcgcg gcttggctac
     1141 cctctgtacc gaccattgta tgacgtgtga agccctggtc ataagggcca tgaggacttg
     1201 acgtcatccc caccttcctc cggcttgtca ccggcagtct cattagagtg cccaactgaa
     1261 tgatggcaac taatgacaag ggttgcgctc gttgcgggac ttaacccaac atctcacgac
     1321 acgagctgac gacagccatg cagcacctgt gttacggctc ccgaaggcac tcctccgtct
     1381 ccggaggatt ccgtacatgt caagaccagg taaggttctt cgcgttgcat cgaattaatc
     1441 cacatcatcc accgcttgtg cgggtccccg tcaattcctt tgagttttaa tcttgcgacc
     1501 gtactcccca ggcggtcaat ttcacgcgtt agctacgcta ccaagcaatc aggttgccca
     1561 acagctaatt gacatcgttt agggcgtgga ctaccagggt atctaatcct gtttgctacc
     1621 cacgctttcg ggcatgaacg tcagtgttgt cccaggaggc tgccttcgcc atcggtattc
     1681 ctccacatct ctacgcattt cactgctaca cgtggaattc tacctccctc tgacacactc
     1741 gagtcaccca gttcagaacg cagttcccgg gttgagcccg gggatttcac atcctgctta
     1801 agtaaccgtc tgcgcccgct ttacgcccag taattccgat taacgctcgc accctacgta
     1861 ttaccgcggc tgctggcacg tagttagccg gtgcttattc ttcaggtacc gtcatcagcc
     1921 gctgatatta gcaacagcct tttcttccct gacaaaagtc ctttacaacc cgaaggcctt
     1981 cttcagacac gcggcatggc tggatcaggc ttgcgcccat tgtccaaaat tccccactgc
     2041 tgcctcccgt aggagtctgg gccgtgtctc agtcccagtg tggcggatca tcctctcaga
     2101 cccgctactg atcgtcgcct tggtaggcct ttaccccacc aaccagctaa tcagatatcg
     2161 gccgctcgaa tagcgcaagg cccgaaagtc ccctgctttc tctctcaaga cgtatgcggt
     2221 attagctgat ctttcgatca gttatccccc actactcggt acgttccgat atgttactca
     2281 cccgttcgcc actcgccacc cgagaagcaa gcttctctgt gctgccgtcc gacttgcatg
     2341 tgtaaagcat gccgccagcg ttcaatctga gccaggatca aactcttatg ttcaatctct
     2401 aactttttaa cttctggtct gcttcaaaga aaccaacagg acaatgttca aaacattatc
     2461 ttgtctgtct ttcaaacagt gtgaggctca aggcactcac acttatcggt aa
//
LOCUS       DAXHSF010000201         2505 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.201, whole genome shotgun sequence.
ACCESSION   DAXHSF010000201 DAXHSF010000000
VERSION     DAXHSF010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.201"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(13..819)
                     /locus_tag="ACFM8S_001931"
     CDS             complement(13..819)
                     /locus_tag="ACFM8S_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFC6314944.1"
                     /translation="MPTVFIFLTAVYAGLGAFAWHCQQQGLGRDYPWKTELPVLGAAL
                     TVHGAALLMPVIQDKIIIMGFGYSGSLIVWMMLFIYFAGSFFYSLRGVQLLLYPCAAL
                     MLLSGLVFPGKFSGYEITDLPFMLHIGTSLLAYGLFGIATLLSVLTLLLNRSLHRRNF
                     SKLAGFLPSLLSLEKLMFQAMWAGFILLTYSVVSGTFFAEAVFGKPMTFTHKTVFGIL
                     SWLIYGGLLLKHSMTAWRGKKAAVWTIIGFVSLMIAYMGSKFVLEIILKR"
     gene            1040..2410
                     /gene="ffh"
                     /locus_tag="ACFM8S_001932"
     CDS             1040..2410
                     /gene="ffh"
                     /locus_tag="ACFM8S_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFC6314945.1"
                     /translation="MLDNLTGRFSNVLKNIRGQAKLTEDNIKEALREVRLALLEADVA
                     LPVVKEFINNVKEKALGQEVAGSLTPDQAFIGVVNKALTELMGRENKTLDLSVAPPAV
                     VLMAGLQGAGKTTTVGKLARLLKNDQKKKVLVVSADVYRPAAIEQLRLLAEQVGVDFF
                     PSDTNQKPVEIATAAVDYAKKHFYDVLMVDTAGRLAIDEEMMNEIKALHAAVNPVETL
                     FVIDAMLGQDAVNTAQAFNEALPLTGVVLTKMDGDSRGGAALSVRHVTGKPIKFIGVG
                     EKINGLEPFHPDRLASRILGMGDVLTLIEDVQKGIDEEAAAKMAKKLHKGKGFDLNDF
                     KEQIQQMRNMGGLENLMSKMPGELGQISKQIPEGTAEKAMGKVEAIINSMTPKERANP
                     ALLKASRKRRIAMGAGTTVQEVNKLLKQFEQMQQMMKMFSGNGLGKLMRLAKGMKGMK
                     GMFPGL"
BASE COUNT          700 a          629 c          643 g          533 t
ORIGIN      
        1 tgttggctct tcttatcttt tcagaatgat ttccaatacg aacttgctgc ccatataggc
       61 aatcataagg ctgacaaatc cgatgatggt ccacacggcg gcttttttac cgcgccatgc
      121 ggtcatgctg tgcttgagca gcagtccgcc gtaaatcagc catgacaata tgccgaatac
      181 ggttttatgg gtaaaggtca tgggtttgcc gaatacggct tcggcaaaaa atgttccgct
      241 gacgacggaa taagtcagca ggatgaaacc tgcccacatg gcctggaaca tgagtttttc
      301 caaactgagc agcgacggca ggaatcctgc gagcttggag aagttcctgc ggtgcaggct
      361 ccgattcagc agcagggtca aaacggacaa taatgttgcg atgccgaaca gcccgtatgc
      421 gagcagcgaa gttccgatat gcagcataaa gggaaggtcg gtaatttcat atcccgagaa
      481 ttttccagga aaaaccaaac ctgacagcag catcagtgcg gcgcaaggat acagcagcaa
      541 ctgcactccg cgcagcgaat aaaagaagct gccggcaaaa taaataaaca gcatcatcca
      601 aacaatcagg ctgccggaat acccaaagcc cataatgatg attttgtctt gaatgaccgg
      661 cataagcagt gccgcgccat ggacggtcaa tgccgcgccc aaaaccggca attccgtctt
      721 ccacgggtaa tcccggccga gcccctgctg ttggcagtgc catgcaaatg cacccaatcc
      781 tgcgtaaacc gccgtcaaaa agatgaaaac tgtcggcatg gtggactttc tctaatctat
      841 actgttgcgc tgtatgcggc cgcttatgaa atattggaac ttttaacgtt ggaattgtaa
      901 aatccccgtt tcgggcaagc cttgacggat ttgccgatat gctgtccggc acacaagccg
      961 catcaaatta ttttgatttt attttaacaa agaatgcccc tgatggggca agctattctt
     1021 attcagacca aggaccagta tgttagacaa tttaaccggc cgcttcagca atgttctcaa
     1081 aaacatccgc gggcaggcca aactgaccga agacaatatt aaagaagcct tgcgcgaagt
     1141 ccgcctcgcc ctgcttgagg cggatgtcgc cctgcctgtc gtcaaagagt tcatcaacaa
     1201 cgtcaaagaa aaggccctcg gtcaggaagt agcgggcagc ctgacgccgg atcaggcatt
     1261 tatcggcgtg gtcaacaaag ccctgaccga actgatgggc agggaaaaca aaacgctgga
     1321 tttgtcggtt gcgccacccg ccgtcgtgtt gatggcaggt ttgcagggcg caggcaagac
     1381 gacgaccgtc ggcaaactcg cccgcctgtt gaaaaacgat cagaagaaaa aggttttggt
     1441 ggtatccgcc gacgtttacc gtcctgccgc gattgaacag ctgcgtctgt tggccgaaca
     1501 ggtcggcgtg gattttttcc cgtccgatac caaccaaaaa ccggttgaaa ttgcaactgc
     1561 cgccgtcgat tacgccaaaa aacattttta cgatgtattg atggtcgata ccgccggccg
     1621 tttggcaatc gatgaagaga tgatgaacga aatcaaagcc cttcacgcgg cggttaaccc
     1681 ggtggaaact ttgttcgtca tcgatgcgat gctaggtcag gatgcggtga acactgctca
     1741 ggcatttaat gaagccctgc cgctgaccgg tgtcgtattg accaagatgg acggcgattc
     1801 gcgcggcggc gcagctttgt ccgtgcgcca cgtaaccggc aagccgatta aatttatcgg
     1861 tgtcggcgaa aaaatcaacg gcctcgaacc tttccacccc gaccgtcttg ccagccgtat
     1921 cctcggcatg ggcgacgtat tgaccctgat tgaagacgtc caaaaaggta ttgatgaaga
     1981 agccgccgcc aaaatggcga aaaagctgca caaaggcaaa ggcttcgacc tcaacgactt
     2041 taaagaacaa atccagcaaa tgcgcaatat gggcggtttg gaaaacctga tgtcgaaaat
     2101 gccgggcgaa ctgggtcaaa tctcgaaaca aatccccgaa ggaacggctg aaaaagcgat
     2161 gggcaaagta gaagccatca tcaactcgat gacccctaaa gaacgcgcca accctgccct
     2221 gctcaaagcc agccgcaaac gccgtattgc aatgggtgcg ggcacgaccg tacaggaagt
     2281 gaacaaattg ctcaaacagt ttgaacaaat gcaacaaatg atgaagatgt tcagcggcaa
     2341 cggcttgggc aaactgatgc gcttggccaa aggcatgaag ggaatgaaag gaatgttccc
     2401 gggtttgtaa gccgatttga ccgaaaacgc cgtctaaaat ttcagacggc gtttttgttt
     2461 tatattctga tttatagtgg attaacaaaa atcaggacaa ggcgg
//
LOCUS       DAXHSF010000202         2498 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.202, whole genome shotgun sequence.
ACCESSION   DAXHSF010000202 DAXHSF010000000
VERSION     DAXHSF010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.202"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(227..1861)
                     /gene="groL"
                     /locus_tag="ACFM8S_001933"
     CDS             complement(227..1861)
                     /gene="groL"
                     /locus_tag="ACFM8S_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFC6314946.1"
                     /translation="MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAF
                     GGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAE
                     GMKYVTAGMNPTDLKRGIDKAVAALVDELKNIAKPCDTSKEIAQVGSISANSDEQVGA
                     IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAALDNP
                     FVLLFDKKISNIRDLLPVLEQVAKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLQDIAILTGGTVIAEEVGLSLEKATLDDLGQAKRIEIGKENTTIID
                     GFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KDRVEDALHATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAVESP
                     LRQIVANAGGEPSVVVNKVLEGKGNYGYNAGSGEYGDMIEMGVLDPAKVTRSALQHAA
                     SIAGLMLTTDCMIAEIPEEKPAMPDMGGMGGMGGMM"
     gene            complement(1954..2244)
                     /locus_tag="ACFM8S_001934"
     CDS             complement(1954..2244)
                     /locus_tag="ACFM8S_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFC6314947.1"
                     /translation="MTIRPLHDRVVVKRLEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     AGKIGKDGARRPLDVKVGDKIIFGKYSGQTVKADGEELLVMREEDIFGIVEK"
BASE COUNT          513 a          658 c          648 g          679 t
ORIGIN      
        1 ttcgcgggaa tgacggcgga aaggttgctg tttttccgat aaattcctgc cgctcttcgt
       61 ttttgggatg gcgggaaata aaacaaaagc gcgcgtatca aaaaataaca atgcaaagaa
      121 cggtgttggc aattttaagg tttgcgcctt agccgcactt attcgcaaaa aaaccgcacg
      181 gcgttgaccg tgcggcttgt tgtctgaagg cttcagacgg cattgcttac atcatgccac
      241 ccataccgcc cataccgccc atgtcaggca tagcaggttt ttcttcaggg atttcagcga
      301 tcatgcaatc agtggtcagc atcaggccgg cgatagatgc ggcgtgttgc agcgcggaac
      361 gggttacttt ggcagggtcg agtacgccca tttcgatcat gtcgccgtat tcgccggagc
      421 cagcgttgta accgtagttg cctttgcctt ccaacacttt gttcacgact acgctaggct
      481 cgccgcctgc gttggcaacg atttgacgca gcggagactc aacggcgcgc aatacgattt
      541 gtacgcctgc gtcttggtcg gcattgccgg tgtgcaggtt ttccagagca gcacgggcgc
      601 gcaacagggc tacgccgccg ccggcaacca caccttcttc aacggctgcg cgggtagcgt
      661 gcagcgcgtc ttccacgcgg tcttttttct ctttcatttc gacttcggta gccgcgccga
      721 ctttgatcac tgccacgccg cctgccagtt tggcaacgcg ctcttgcagt ttttctttgt
      781 cgtaatcgct ggttgcggtt tcgatttgtt ggcggatttc ggcaacacgc gcttcgattt
      841 gggcagcgtc accgaagccg tcgatgatgg tggtgttttc tttaccgatt tcgatgcgtt
      901 tggcttgacc caagtcgtcc aaagtcgctt tttccagaga caggccgact tcttcggcaa
      961 ttacggtgcc gccggtcagg atggcgatgt cttgcaacat cgctttgcgg cggtcgccga
     1021 agcccggagc tttgacggca acggttttca ggatgcctcg gatgttgttc accaccaaag
     1081 tcgccaaggc ttcgccttct acgtcttcag cgataatcaa gagcggacgg ctggcttttg
     1141 ccacttgttc caacacaggc aacaagtcgc ggatgttgct gatttttttg tcgaacaaca
     1201 acacaaacgg attgtccaaa gctgcaattt gtttttccgc atcgttgatg aagtaaggag
     1261 acaggtagcc tcggtcgaac tgcatacctt caaccacgtc cagctcgttt tccaaagatt
     1321 tgccgtcttc aacggtaatc acgccttctt tgccgacttt ttccatcgct tcggcgataa
     1381 tcgcgccgac ttgttcgtca gagttggcgg aaatagagcc gacttgggcg atttctttag
     1441 aagtgtcgca aggtttggcg atatttttca gctcgtcaac caaagcggcg acggctttgt
     1501 cgataccgcg tttcaggtcg gtcggattca tacctgcggt aacatatttc ataccttcgg
     1561 caacgatgga ttgcgccagt acggtggcgg tagtcgtacc gtcgcccgcc acgtcgttgg
     1621 ttttggacgc gacttctttc accatttgcg cgcccatatt ttcaaacttg tctttcagtt
     1681 cgatttcttt ggcgacggtt acgccgtctt tagtaatgtg cgggccgccg aaagcgcggt
     1741 caaccaccac gttgcggcct ttggggccca aagttacgcg cacggcgttt gccagaatgt
     1801 tcacgccgtt taccattttt tggcggactt cattgccgaa ctgtacgtct tttgctgcca
     1861 tttcaattct ccaaaaatca ttaaaactgt ctgataaaac cgtttatgcc gtctgaaggc
     1921 ggtttgccgt ttcagacggc atcgtgtccg tatttatttt tcaacgatgc cgaaaatatc
     1981 ttcttcgcgc attaccaaca gctcttcgcc gtcggctttt acggtttggc cgctgtattt
     2041 gccgaagatg attttgtcgc cgactttgac atccagcgga cggcgcgcgc cgtctttacc
     2101 gattttgccc gcgcccacgg cgatgacttc gcccatgtcg ggtttttcgg cggccgcacc
     2161 cggcaaaacg atgcccgatg cggttttctc ttcagcttcc aagcgtttga cgacaacgcg
     2221 gtcgtgtaaa ggacggatgg tcatatttat gctccgataa aatgatttga aaacaatcat
     2281 ctgcccgaac ggttcggaca gattgaagtg gaaaccgggc tgccgcctgc cggcccgccc
     2341 gtataagtcg gcaaaattag ggtgtgtgcg ggcaaattca agtgaggcgg aaaaaattta
     2401 tttccgccgt tttttatagt ggactaaatt taaggggctg tactagatta gcagatatgt
     2461 taccctcgaa atatgaagat aacacactgc aaattaaa
//
LOCUS       DAXHSF010000203         2492 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.203, whole genome shotgun sequence.
ACCESSION   DAXHSF010000203 DAXHSF010000000
VERSION     DAXHSF010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.203"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            167..790
                     /gene="lolA"
                     /locus_tag="ACFM8S_001935"
     CDS             167..790
                     /gene="lolA"
                     /locus_tag="ACFM8S_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFC6314948.1"
                     /translation="MMKPHNLFQFLAVCSLTVSVASAQAGAVDALKQFNNDADGISGS
                     FTQTVQSKKKTQTAHGTFKILRPGLFKWEYTSPYKQTIVGDGQTVWLYDVDLAQVTKS
                     SQDQAIGGSPAAILSNKTALESSYTLKEDGSSNGIDYVLATPKRNNAGYQYIRIGFKG
                     GNLAAMQLKDSFGNQTSISFGGLNTNPQLSRGAFKFTPPKGVDVLSN"
     gene            1063..2199
                     /locus_tag="ACFM8S_001936"
     CDS             1063..2199
                     /locus_tag="ACFM8S_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6314949.1"
                     /translation="MKKTLVAAILSLALTACGGGNDTAAQAPSAKPEAEQSGKLNIYN
                     WSDYVDPETVAAFEKETGIKTRSDYYDSNETLEAKVLTGKSGYDLTAPSIANVGRQIK
                     AGAYQKIDKAQIPHYGNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINTRQVQKALG
                     TDKLPENEWDLVFNPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKAA
                     VDMMKAVRGDVKRFSSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEEAANGVEIKVLT
                     PKTGVGVWVDSFMIPRDAQNVANAHRYIDYTLRPEVAAKNGSFVTYAPASRPARELMD
                     EKYTSDASIFPNKELMEKSFIVSPKSAESAKLGVKLWQGLKAGK"
     gene            2331..>2492
                     /locus_tag="ACFM8S_001937"
     CDS             2331..>2492
                     /locus_tag="ACFM8S_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC6314950.1"
                     /translation="MDLSIVVPIYNVESYLEACLNSIEPILSNENVELILVNDGSKDG
                     SEDICYKYID"
BASE COUNT          678 a          730 c          611 g          473 t
ORIGIN      
        1 cggcacacaa ttaaagggca gcgtggcgcg ccgccttttc cggtgggcaa aaaatcagcc
       61 ctcggaaaac gcggtttgca aaatgcaaac cgcccgtaac gccgcccata tgattgtttt
      121 gccgcgccga tactttacgc cacacttatt ccaacaagga aaaataatga tgaaaccgca
      181 caacctgttc caattcctcg ccgtttgctc cctgaccgtc tccgtcgctt ccgcacaggc
      241 gggcgcggtg gacgcgctca agcaattcaa caacgatgcc gacggtatca gcggcagctt
      301 cacccaaacc gtccaaagca aaaagaaaac ccaaaccgcg cacggcacgt tcaaaatcct
      361 gcgcccgggc ctcttcaaat gggaatacac ttcgccctac aaacagacta ttgtcggcga
      421 cggtcaaacc gtttggctct acgatgttga tttggcacaa gtgaccaagt cgtcccaaga
      481 ccaggccata ggcggcagcc ccgccgccat cctgtcgaac aaaaccgccc tcgaaagcag
      541 ctacacgctg aaagaggacg gttcgtccaa cggcatcgat tatgtgctgg caacgcccaa
      601 acgcaacaat gccggctacc aatacatccg catcggcttc aaaggcggca acctcgccgc
      661 catgcagctt aaagacagct tcggcaacca aacctccatc agtttcggcg gtttgaatac
      721 caatccccaa ctctcgcgcg gcgcgttcaa gtttaccccg cccaaaggcg tggacgtgtt
      781 gagcaactga tgccgtccgc cccgatgccg tctgaaagcc gccgaggctt cagacggcat
      841 tttcacgcag gcggaacaat gtcccacatt accgcccgat cgggcatcgg aacggcaaac
      901 ccgtgaaaat taacggttgc gccccggctg tttttgccgt ttaatgcaaa ccttgctgca
      961 ccaagggcca agaaagccga ccggccgccc ccacagcttc cgatgcaggc ggcccggccg
     1021 tccctgcaat gttttttatt tttgaacgaa aggtcgaaaa ccatgaaaaa aacactggtg
     1081 gcggcaatcc tgagcctcgc cttgactgcg tgcggcggcg gaaacgatac cgccgcccaa
     1141 gccccctccg ccaagcccga agccgaacaa tcgggcaaac tcaatatcta caactggtcg
     1201 gattatgtcg atcccgaaac cgtcgccgcc tttgaaaaag aaaccggcat caagacgcgt
     1261 tccgattatt acgacagcaa cgaaacacta gaggcaaaag tcctgaccgg caaatccggc
     1321 tacgacctga ccgcgccgtc catcgccaac gtcggccggc aaatcaaagc gggcgcgtat
     1381 cagaaaatcg acaaggcgca aatcccccat tacggcaaca tcgataaaga tttgctgaaa
     1441 atgatggaag ccgtcgatcc gggcaacgaa tacgccgtcc cctatttctg gggcatcaac
     1501 accttggcaa tcaacacgcg gcaggtgcaa aaggcattgg gtacggacaa gctgcccgaa
     1561 aacgaatggg atttggtgtt caaccctgaa tacaccgcca aactcaaatc ctgcggcatc
     1621 agctatttcg acagcgcaat cgaacagatt cctttggcgt tgcactattt gggcaaagac
     1681 cccaacagtg aaaatcccga agacatcaaa gccgccgtcg atatgatgaa agccgtccgg
     1741 ggcgacgtga aacgcttcag ctcttccggc tatatcgacg atatggcggc gggcaacctg
     1801 tgtgccgcca tcggttacgg cggcgatttg aacattgcca aaacccgtgc cgaagaagcc
     1861 gcaaacggcg tggaaatcaa agtattgacc ccgaaaaccg gcgtgggcgt gtgggtggat
     1921 tcctttatga ttccgcgcga cgcacaaaac gttgccaacg cccaccgcta tatcgactac
     1981 acgctccggc ccgaggtggc ggcaaaaaac ggcagcttcg ttacctacgc gcccgccagc
     2041 cgtcccgcgc gcgagctgat ggatgaaaaa tacacctccg acgcatcgat tttcccgaac
     2101 aaagaactga tggaaaaaag tttcatcgta tcgcccaaat ccgcagaatc cgccaaactg
     2161 ggcgtgaagc tgtggcaagg gctcaaggcc ggaaaataac cgcaacctct gccgtctgaa
     2221 acctttcggg cggcaggaaa cggcgcgtcc gttatcaaac agggggcgtt tccccgcccg
     2281 cctgttatga ttgggttaag attaaaatga tttagtaaaa tgagaaagat atggatttaa
     2341 gtatcgtagt tcccatttat aatgtcgaga gttatttgga agcgtgtcta aattccatag
     2401 aacctatatt aagtaatgaa aatgtcgaac ttatccttgt gaatgacggg tcaaaagacg
     2461 gaagtgaaga tatatgttat aaatatatag at
//
LOCUS       DAXHSF010000204         2469 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.204, whole genome shotgun sequence.
ACCESSION   DAXHSF010000204 DAXHSF010000000
VERSION     DAXHSF010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.204"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            230..2407
                     /gene="fetA"
                     /locus_tag="ACFM8S_001938"
                     /gene_synonym="frpB"
     CDS             230..2407
                     /gene="fetA"
                     /locus_tag="ACFM8S_001938"
                     /gene_synonym="frpB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021438799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor FetA/FrpB"
                     /protein_id="HFC6314951.1"
                     /translation="MNTPLFRLSLLSLTLAAGFAHAAENNAKVVLDTVTVKGDRQGSK
                     IRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDNA
                     YSDSQILYHQGRFIVDPALVKVVSVQKGAGSASAGIGATNGAIIAKTVDAQDLLKGLD
                     KNWGVRLNSGFASNEGVSYGASVFGKEGNFDGLFSYNRNDEKDYEAGKGFRNVNGGKT
                     VPYSALDKRSYLAKIGTTFGDDDHRIVLSHMKDQHRGIRTVREEFTVGDKSSRINIDR
                     QAPAYRETTQSNTNLEYTGKNLGFVEKLDANAYVLEKKRYSADDKGSGYAGNVEGPNH
                     TRITTRGMNFNFDSRLAEQTLLKYGINYRHQEIKPQAFLNGDFTISSKKKDPNNPKNE
                     IDKDPTEIAKDKEDMALVHSYKLSNPTKTDAGVYVEAIHDIGDFTLTGGLRYDRFKVK
                     THDGKTVSSSSLNPSFGVIWQPHEHWSFSASHNYASRSPRLYDALQTHGKRGIISIAD
                     GTKAERARNTEIGFNYNDGTFAANGSYFWQTIKDALANPQNRHDSVAVREAVNAGYIK
                     NHGYELGASYRTGGLTAKVGVSYSKPRFYGYDTHKDKLLSANPEFGAQVGRTWTASLA
                     YRFQNPNLEIGWRGRYVQKAVGSILVSGQKDRQGNLENVVRKGFGVNDVFANWKPLGK
                     DTLNVNLSVNNVFNKYYYPHSQRWTNTLPGVGRDVRLGVNYKF"
BASE COUNT          711 a          713 c          555 g          490 t
ORIGIN      
        1 caggcttcgt cattcccgca tttgttaatc cactatattt ccgccgtttt ttacatttcc
       61 gacaaaacct gtcaacaaaa aacaacactt cgcaaataaa aacgataatc agctttacac
      121 aaatcccccc tctcgttaat ataaacaaaa ataattatta tttttcttat cctgccaaac
      181 cttaacggtt tggcttaact tcccttcata tactcaagag gacgattgaa tgaatacccc
      241 attgttccgt ctcagcctgc tctcgcttac cctggcggca ggttttgccc acgcggcaga
      301 aaataatgcc aaggtcgtac tggataccgt tactgtaaaa ggcgaccgcc aaggcagcaa
      361 aatccgtacc aacatcgtta cgctgcaaca aaaagacgaa agcaccgcaa ccgatatgcg
      421 cgaactctta aaagaagagc cctccatcga tttcggcggc ggcaacggca cgtcccaatt
      481 cctgacgctg cgcggcatgg gtcagaactc tgtcgacatc aaggtggaca acgcctattc
      541 cgacagccaa atcctttacc accaaggcag atttattgtc gatcccgctt tggttaaagt
      601 cgtttccgta caaaaaggcg cgggttccgc ctctgccggt atcggcgcga ccaacggcgc
      661 gattatcgcc aaaaccgtcg atgcccaaga cctgctcaaa ggcttggata aaaactgggg
      721 cgtgcgcctc aacagcggct ttgccagcaa cgaaggcgta agctacggcg ccagcgtatt
      781 cggaaaagag ggcaacttcg acggcttgtt ctcttacaac cgcaacgatg aaaaagatta
      841 cgaagccggc aaaggtttcc gcaatgtcaa cggcggcaaa accgtaccgt acagcgcgct
      901 ggacaaacgc agctacctcg ccaaaatcgg aacaactttc ggcgacgacg accaccgcat
      961 cgtgttgagc cacatgaaag accaacaccg gggcatccgc actgtgcgtg aagaatttac
     1021 cgtcggcgac aaaagttcac ggataaatat tgaccgccaa gcccctgctt accgcgaaac
     1081 cacccaatcc aacaccaact tggagtacac gggtaaaaac ctgggctttg tcgaaaaact
     1141 ggatgccaac gcctatgtat tggaaaagaa acgctattcc gccgatgaca aaggcagcgg
     1201 ttacgcaggc aatgtagaag gccccaacca tacccgaatc accactcggg gtatgaattt
     1261 caacttcgac agccgccttg ccgaacaaac cctgttgaaa tacggtatca actaccgcca
     1321 tcaggaaatc aaaccgcaag cgtttttgaa cggcgacttt acgatttcca gtaagaaaaa
     1381 agacccaaac aatcctaaaa acgaaataga taaagaccct acagaaatag cgaaagacaa
     1441 ggaagatatg gctcttgtcc attcctacaa actgtccaac ccgaccaaaa ccgatgccgg
     1501 cgtatatgtt gaagccattc acgacatcgg cgatttcacg ctgaccggcg ggctgcgtta
     1561 cgaccgcttt aaggtgaaaa cccacgacgg caaaactgtt tcaagcagca gccttaaccc
     1621 gagtttcggc gtgatttggc agccgcacga acactggagc ttcagcgcaa gccacaacta
     1681 cgccagccgc agcccgcgcc tgtatgacgc gctgcaaacc cacggcaaac gcggcatcat
     1741 ctcgattgcc gacggcacca aagccgaacg cgcgcgcaat accgaaatcg gcttcaacta
     1801 caacgacggc acgtttgccg caaacggcag ctacttctgg caaaccatca aagacgcgct
     1861 tgccaatccg caaaaccgcc acgactctgt cgccgtccgt gaagccgtca atgccggtta
     1921 catcaaaaac cacggttacg aattgggcgc gtcctaccgc accggcggcc tgactgccaa
     1981 agtcggcgta agctacagca aaccgcgctt ttacggttac gatacgcaca aagacaagct
     2041 gttgagcgcg aaccctgaat ttggcgcaca agtcggtcgc acttggacag cctcccttgc
     2101 ctaccgcttc caaaatccga atctggaaat cggctggcgc ggacgctatg ttcaaaaagc
     2161 cgtgggttcg atattggtgt caggtcaaaa agaccgccaa ggcaacttgg aaaacgttgt
     2221 acgcaaaggt ttcggtgtga acgatgtctt cgccaactgg aaaccattgg gcaaagacac
     2281 gctcaatgtc aatctttcgg ttaacaacgt gttcaacaag tactactatc cgcacagcca
     2341 acgctggacc aataccctgc cgggcgtggg acgtgatgta cgcttgggcg tgaactacaa
     2401 gttctaaaac gcacatcccg aaaaaatgcc gtctgaaagc ctttcagacg gcatctgttc
     2461 tgataattt
//
LOCUS       DAXHSF010000205         2467 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.205, whole genome shotgun sequence.
ACCESSION   DAXHSF010000205 DAXHSF010000000
VERSION     DAXHSF010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.205"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            35..1390
                     /locus_tag="ACFM8S_001939"
     CDS             35..1390
                     /locus_tag="ACFM8S_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC45 family MFS transporter"
                     /protein_id="HFC6314952.1"
                     /translation="MSEYTPQTAKQGLPALAKSTIWMLSFGFLGVQTAFTLQSSQMSR
                     IFQTLGADPHNLGWFFILPPLAGMLVQPIVGHYSDRTWKPRLGGRRLPYLLYGTLIAV
                     IVMILMPNSGSFGFGYASLAALSFGALMIALLDVSSNMAMQPFKMMVGDMVNEEQKGY
                     AYGIQSFLANTGAVVAAILPFVFAYIGLANTAEKGVVPQTVVVAFYVGAALLVITSAF
                     TIFKVKEYDPETYARYHGIDVAANQEKANWIELLKTAPKAFWTVTLVQFFCWFAFQYM
                     WTYSAGAIAENVWHTTDASSVGYQEAGNWYGVLAAVQSVAAVICSFVLAKVPNKYHKA
                     GYFGCLALGALGFFSVFFIGNQYALVLSYTLIGIAWAGIITYPLTIVTNALSGKHMGT
                     YLGLFNGSICMPQIVASLLSFVLFPMLGGLQATMFLVGGVVLLLGAFSVFLIKETHGG
                     V"
     gene            1394..2413
                     /locus_tag="ACFM8S_001940"
     CDS             1394..2413
                     /locus_tag="ACFM8S_001940"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFC6314953.1"
                     /translation="MSDTPATPATRDFGLIDGRAVTSYVLSNRRGTRVCVLDLGGIVQ
                     EFSVLADGVRENLVVSFDDAASYADNPFQINKQIGRVAGRIRGAAFDINGRTYRVEAN
                     EGRNALHGGSHGLAVTRFNAVAADGRSVVLRSRLQQSADGYPNDLDLDISYRLDEDDR
                     LTVSYRATALGDTVFDPTLHIYWRLDAGLHDAVLHIPQGGHIPADAEKLPVSTVSDDL
                     EVFDFSRPKPLDAAVAALRRETGRAGFDDAYRVPSDIGRPAAVLQAGRRRRISIYSDR
                     NGLVIFTAAPQDFARHDAGVYDALATEAQTLPDSLNWPEFGNIRLNKGDTREATIAYG
                     IESLS"
BASE COUNT          456 a          669 c          740 g          602 t
ORIGIN      
        1 actatacttt tcaaatcaaa aaaggattta ccttatgtcg gaatatacgc ctcaaacagc
       61 aaaacaaggt ttgcccgcgc tggcaaaaag cacgatttgg atgctcagtt tcggctttct
      121 cggcgttcag acggccttta ccctgcaaag ctcgcaaatg agccgcattt ttcaaacgct
      181 aggcgcagac ccgcacaatt tgggctggtt tttcatcctg ccgccgctgg cggggatgct
      241 ggtgcagccg attgtcggcc attactccga ccgcacttgg aagccgcgtt tgggcggccg
      301 ccgtctgccg tatctgcttt atggcacgct gattgcggtt attgtgatga ttttgatgcc
      361 gaactcgggc agcttcggtt tcggctatgc gtcgctggcg gctttgtcgt tcggcgcgct
      421 gatgattgcg ctgttggacg tgtcgtcaaa tatggcgatg cagccgttta agatgatggt
      481 cggcgacatg gtcaacgagg agcagaaagg ctacgcctac gggattcaaa gtttcttagc
      541 aaatacgggc gcggtcgtgg cggcgattct gccgtttgtg tttgcgtata tcggtttggc
      601 gaacaccgcc gagaaaggcg ttgtgccgca gaccgtggtc gtggcgtttt atgtgggtgc
      661 ggcgttgctg gtgattacca gcgcgttcac gattttcaaa gtgaaggaat acgatccgga
      721 aacctacgcc cgttaccacg gcatcgatgt cgccgcgaat caggaaaaag ccaactggat
      781 cgaactcttg aaaaccgcgc ctaaggcgtt ttggacggtt actttggtgc aattcttctg
      841 ctggttcgcc ttccaatata tgtggactta ctcggcaggc gcgattgcgg aaaacgtctg
      901 gcacaccacc gatgcgtctt ccgtaggtta ccaagaagcg ggcaactggt acggcgtttt
      961 ggcggcggtg cagtcggttg cggcggtgat ttgttcgttt gtattggcga aagtgccgaa
     1021 taaataccat aaggcgggtt atttcggctg tttggctttg ggcgcgctcg gctttttctc
     1081 cgttttcttc atcggcaacc aatacgcgct ggtgttgtct tataccttaa tcggcatcgc
     1141 ttgggcgggc attatcactt atccgctgac gattgtgacc aacgccttgt cgggcaagca
     1201 tatgggcact tacttgggcc tgtttaacgg ctctatctgt atgcctcaaa tcgtcgcttc
     1261 gctgttgagt ttcgtgcttt tccctatgct gggcggcttg caggccacta tgttcttggt
     1321 agggggcgtc gtcctgctgc tgggcgcgtt ttccgtgttc ctgattaaag aaacacacgg
     1381 cggggtttga gcgatgagcg atacccccgc tacccccgct acccgcgatt tcggcctgat
     1441 cgacgggcgt gccgtaacca gctatgtgct gtccaatcgg cgtggtacgc gtgtctgcgt
     1501 gctggacttg ggcgggattg tgcaggaatt ttccgttttg gcagacggcg tgcgcgaaaa
     1561 cctcgtggtg tcgttcgacg atgcggcttc ctatgcggac aatccgtttc agattaacaa
     1621 gcagataggg cgcgtggccg gacgcatccg cggtgcggcg ttcgacatca acggcaggac
     1681 ttaccgcgtg gaggccaacg aaggcaggaa cgcgctgcac ggcggttcgc acgggctggc
     1741 cgttacccgt ttcaacgcgg tggcggcaga cggccgttcg gtggtgctgc gcagccgcct
     1801 gcaacagtcg gccgacggtt atcccaacga tttggatttg gatatttcct accgcttgga
     1861 cgaggacgac cggcttaccg ttagctatcg cgccaccgcg ctcggcgaca cggtgttcga
     1921 cccgacgctg cacatttact ggcggctgga cgcgggcctg cacgatgcgg ttctgcatat
     1981 tccgcagggc ggacatattc cggccgatgc cgaaaaactg cccgtctcaa cggtttcaga
     2041 cgacctcgaa gtatttgatt tcagccggcc caagccgctg gatgccgccg ttgccgccct
     2101 gcgccgcgaa acgggtcggg ccggttttga cgacgcttac cgcgtgccgt ccgatatagg
     2161 ccgtcccgcc gctgtgttgc aagccggacg ccgccgtcgt atcagcatat acagcgaccg
     2221 caacggcttg gtcatcttta ccgccgcccc gcaggatttc gcgcggcacg atgcgggcgt
     2281 ttacgacgcg ctggcgaccg aggcgcagac gctgcccgac agcctgaatt ggcccgagtt
     2341 cggcaatatt cgtctgaaca agggtgatac tagggaggcg acgattgctt acggcatcga
     2401 atccctttct taggagcttc ctaacaccgg ttgcagacgg cctttttata gtggattaac
     2461 aaaaacc
//
LOCUS       DAXHSF010000206         2449 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.206, whole genome shotgun sequence.
ACCESSION   DAXHSF010000206 DAXHSF010000000
VERSION     DAXHSF010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.206"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..567
                     /locus_tag="ACFM8S_001941"
     CDS             <1..567
                     /locus_tag="ACFM8S_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFC6314954.1"
                     /translation="PVAYGKIKVQSWKERRDFNIVKQDLDFSCGAASVVTLLNNFYGQ
                     KLTEEDVLKKLDKEQMPASFEDMRRIMPDLGFEAKGYALSFEQLAQLQISVIVYLKYR
                     KDDHFSVLRGIDGNTVSLADPSLGHVSMSRAQFLDAWQTREGNLAGKILAVVPKGRDA
                     GGDKAFFTRSPKRQTEFAVGQVKWWRAY"
     gene            697..2358
                     /locus_tag="ACFM8S_001942"
     CDS             697..2358
                     /locus_tag="ACFM8S_001942"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HFC6314955.1"
                     /translation="MTESITRDSMQYDVVIVGAGPSGLSAAIKLKQLAEKNGREISVC
                     VVEKGSEAGAHSLAGAVIDPIALNELIPDWKEKGAPLTRAVTQDKVLFLTEKKAFNLP
                     ITPNFDNHGNYIVSLGEVVRWLAEQAENMGVEIYPGFAAAEVLYHEDGSVKGIATGNM
                     GIGKDGEPTDSFQPGMELWAQQTLFAEGCRGSLSKQIIERFKLDQNSQPQTYGLGIKE
                     VWEVPSEQHQPGLVVHSAGWPLDSKTYGGAFVYHFDDNKVAVGFVVGLDYQNPYLSPF
                     EEFQRFKTHPEIRKTFEGGRRIAYGARSLIEGGLQSLPKLSFKGGILVGDAAGFLNMP
                     RIKGIHTAMKSAMLAAEAVFPLLENLEEVEGFDSGKEAADYQQRFEQSWLYQELYAAR
                     NVRPSFKWGVYLGSIYTGIDQMIFRGKAPWTLKHHGKDNEQLKKAAACKPIDYPKPDG
                     VLTFDRLSSVFLANLAHEENQPDHLVLNNPQTMIDVNYKEYASPETRYCPAGVYEIVE
                     ENGNPRLQINAANCVHCKTCDIKDPTQNITWICPEGASGPNYGGM"
BASE COUNT          621 a          602 c          677 g          549 t
ORIGIN      
        1 cctgttgctt atggaaaaat caaagtgcag agttggaaag aaaggcgcga tttcaatatt
       61 gtaaagcagg atttggattt ttcctgcggg gcggcttcgg tggtgacgct tttgaacaat
      121 ttttacgggc aaaagctgac ggaagaggac gtgttaaaaa aactggataa ggagcagatg
      181 cccgcgtcgt ttgaggatat gcggcgcatt atgcctgatt tgggttttga agcgaaaggc
      241 tatgccctgt ctttcgagca gcttgcacag ttgcaaatct ccgtcatcgt gtatctgaaa
      301 taccgcaagg atgatcattt ctcggtattg cgcggtatag acggcaatac ggtttcgctt
      361 gccgacccgt cgctgggtca tgtttcaatg agcagggcgc agtttttgga tgcttggcaa
      421 acccgtgagg gaaatttggc aggtaagatt ttggcggttg tgccgaaagg gagggatgcc
      481 ggcggggata aggcgttttt cacacgcagt ccgaaacgtc agacggagtt tgcggtcgga
      541 caggtaaaat ggtggcgtgc ttattgaaat ttcgacaaag gtcgtctgaa aaccgaaaat
      601 atggttttca gacgaccttt gttgtatttg gtaactatat gttcccgttg tataattacg
      661 gatttgcaat tcaataataa atacacagga accgccatga cagaatccat cacccgcgac
      721 agtatgcaat acgatgtcgt gattgtcggc gcaggcccgt cgggtttgtc cgccgccatc
      781 aaactcaagc agcttgccga aaaaaacggg cgcgaaatca gcgtttgcgt ggtggagaaa
      841 ggttctgaag caggcgcgca ttcgctggcg ggcgcggtta tcgatccgat tgcgctgaat
      901 gagttgattc ccgactggaa agaaaaaggc gcgccgctga cgcgcgcggt aactcaggac
      961 aaagtgttgt tcctgaccga gaaaaaagcg ttcaatctgc ctattacccc caatttcgac
     1021 aatcatggca actacatcgt cagcttgggc gaagtcgtgc gctggttggc ggagcaggcg
     1081 gaaaatatgg gcgtggaaat ctatccgggc tttgccgccg ccgaagtgct gtatcacgaa
     1141 gacggttcgg tcaaaggcat tgcaaccggc aatatgggca ttggcaaaga cggcgagccg
     1201 accgattcat tccaacccgg catggagctt tgggcgcagc aaaccctgtt tgccgaaggt
     1261 tgtcgcggtt cgctttccaa acaaatcatc gaacgcttca aactcgacca aaacagccag
     1321 ccgcaaactt acggcttggg cattaaagaa gtttgggaag tgccgtccga acagcatcag
     1381 cccggtttgg tggtgcacag cgcaggctgg ccgctggaca gcaaaaccta cggcggcgcg
     1441 tttgtttacc atttcgacga caacaaagtc gccgtcggct tcgtggtcgg tttggactat
     1501 caaaatcctt atctgtcgcc gtttgaagag ttccaacgtt tcaaaaccca tcccgaaatc
     1561 cgcaaaacct ttgaaggcgg ccgccgcatt gcttacggcg cgcgttcgct gattgaaggc
     1621 ggtttgcaaa gtttgccgaa gctctcgttc aaaggcggca ttttggtcgg cgatgcggcg
     1681 ggtttcctca atatgccgcg catcaaaggc attcataccg cgatgaaatc cgccatgctc
     1741 gccgccgaag ccgtgttccc cttgttggaa aaccttgaag aagtggaggg tttcgacagc
     1801 ggcaaagagg cggcggatta tcagcaacgt tttgaacaaa gctggctgta tcaagagctt
     1861 tacgccgcgc gcaatgtccg tccgtcattc aaatggggcg tttacctcgg ctcaatctac
     1921 accggcatcg atcagatgat tttcagaggc aaagccccgt ggaccttgaa acatcacggc
     1981 aaagacaacg agcagcttaa aaaagccgcc gcgtgcaagc cgattgatta tccgaaaccc
     2041 gacggcgtgt tgaccttcga ccgcttgagc agcgtcttcc ttgccaatct cgcgcacgaa
     2101 gaaaaccagc ccgaccattt ggtgctgaac aatccgcaaa cgatgataga cgtgaactac
     2161 aaagaatacg cctcgcccga aacgcgctat tgtccggccg gcgtgtacga aattgttgaa
     2221 gaaaacggca atccgcgcct gcaaatcaac gcggcaaact gcgtgcactg caaaacctgc
     2281 gacatcaaag acccgacgca aaacatcact tggatttgcc ccgaaggcgc gagcgggccg
     2341 aattacggcg ggatgtaaac cttattccgc cgttacgccc ggtcaggaat aaggaatggg
     2401 gcagttgttc aaacctgccc cgtttgaacc cataatatag tggattaaa
//
LOCUS       DAXHSF010000207         2400 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.207, whole genome shotgun sequence.
ACCESSION   DAXHSF010000207 DAXHSF010000000
VERSION     DAXHSF010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.207"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            432..507
                     /locus_tag="ACFM8S_001943"
     tRNA            432..507
                     /locus_tag="ACFM8S_001943"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:466..468,aa:Glu,seq:ttc)
     gene            892..1057
                     /locus_tag="ACFM8S_001944"
                     /pseudo
     CDS             892..1057
                     /locus_tag="ACFM8S_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216748.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
     gene            complement(1107..1913)
                     /gene="thiD"
                     /locus_tag="ACFM8S_001945"
     CDS             complement(1107..1913)
                     /gene="thiD"
                     /locus_tag="ACFM8S_001945"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFC6314956.1"
                     /translation="MKSSFVQTLTIAGSDSGGGAGIQADLKTFQMRGVFGTCVITAVT
                     AQNTLGVSAVHLVPTETITAQIQAIREDFDIRAYKIGMLGTAEIIECVADKLKHCDFG
                     RRVLDPVMIAKGGAPLLQDSAVAALTRLLLPDTDVLTPNLPEAEALTGVHIENRKDAE
                     RAAKILLDYGVKNVVIKGGHLNGSTSGRCTDWLFTQNETLELDSPRFPTAHTHGTGCT
                     FSACITAELAKGSDVCEAVRTAKSYITAAISNPLEIGAGHGPVNHWAYRD"
BASE COUNT          577 a          617 c          610 g          596 t
ORIGIN      
        1 atctgaaagt ttgaggttct agattcccgc tttcgcggga atgacgaaaa gtggcgggaa
       61 tgacgaagtt tcagacggca tcgcccgcct gttttgatat ggcggcaccc ccccgacaaa
      121 aaaaacaatc cggaacgcat ctgaccgttc cggcttgttt tcaggcgaat ccgccgcatc
      181 agaacatact gcgcacgccc atattgacct gccaagtctg gcgcatcgtg tgcatcgaag
      241 acctttgcgc ctcaaaataa agctgccttc cgttgtcggc attaccacgc aaaaaaatga
      301 attgattgat attccaatgt tttttatatg tttttatatt gtgatgcgat cagacaaacg
      361 cccccctgac atttgtttag acggcatcgt attgctaaat ttctataagt atgtataatg
      421 tccgtttcca cgcgcccatc gtctagaggc ctaggacact gccctttcac ggcggcaacc
      481 ggggttcgaa tccccgtggg cgtgccaatt caaaaacctg cttgtttcaa gcaggttttt
      541 ttattatgaa tcgtcattcc cgcacccttc gtcattcccg cgaaagcggg aatctggagc
      601 gcaggattaa agaaaccgtt ttatccgata agcttccgtg ccgacaggtc tggattcccg
      661 cctgcgcggg aaggacggca gagggtggac gatgccgtct aaagcctgac aaggcatttg
      721 atgccgtctg aaacttcgtc attcccgcac ccttcgtcat tcccgcaaaa gcgggaatct
      781 agagcgtagg gttgaagaaa ccgttttatc caataagttt ccgtgccgac aggtctggat
      841 tcccactttc gtgggaatga cgagtctttt ataacctgag acctttgcaa aatgcctttc
      901 ccttttaaat ttaaagaggg gggagctgaa ggattattat cgggattggg agctggtcga
      961 atataaagaa gaattgggcg cgatgcacgc caaagacgaa aacggcaatc cgatacggtt
     1021 taaatttgta accatgctgg cgaaaaagcc tgaataatcc tcaaaaatgc cgtctgaaca
     1081 ttttgtttca gacggcattt ttacgtctag tcccgatacg cccaatgatt gaccgggccg
     1141 tgtcccgcgc cgatttccaa agggtttgag attgccgccg taatgtagga cttggcagtc
     1201 cgtacggctt cgcaaacgtc cgagcctttt gccaactcgg cggtgatgca ggcggaaaac
     1261 gtgcagcccg tgccgtgcgt gtgggcggtt ggaaagcgcg ggctgtccaa ttccagcgtt
     1321 tcattttgcg taaacagcca atccgtgcag cgtccgcttg tgctgccgtt caaatgtccg
     1381 cctttgataa cgacattttt gacaccgtaa tcaagcagga tttttgccgc acgttccgca
     1441 tctttacggt tttcgatatg cacgccggtc agggcttccg cctcgggcag gttgggggtc
     1501 aatacatccg tatcgggaag cagcaggcgc gtcagtgccg caacggcgga atcctgcaac
     1561 agcggcgcac cgcctttggc aatcatcaca gggtcgagta cgcgcctgcc gaagtcgcag
     1621 tgtttcagct tgtcggcaac gcattcgatg atttccgccg tgccgagcat tcctattttg
     1681 taggcgcgga tgtcaaagtc ttccctgatt gcttggattt gtgcggtgat ggtttcggtc
     1741 gggacgagat gaaccgccga cacgcccaag gtattttgcg cggtaacggc ggtgatgacg
     1801 cacgttccga acacgccgcg catctgaaac gttttcaaat ccgcctgaat gcccgcaccg
     1861 ccgcccgaat ccgaaccggc gatggtaagc gtttgcacaa aagagctttt catttgttcc
     1921 tccttaatat tgtgtccgat aaaaagggct aatgcctgta aaaaagcacg gcgatgccgt
     1981 ctgaaaaccg cagcgcgggg tttgtggtgc attatatgcc gtattgcagg acaaggcaca
     2041 gggcagggcg gcggggtgca tcgtagcaac tgtatttttg cccgacgggg tgaaaaatac
     2101 agttgctaca cccactgata atgaaaaaag agagcaatgc cgtctgaacc gcgtgttgcg
     2161 gcgagcataa accggcacgc tttattcgtc attcccacaa aaacagaaac ctaaaattcc
     2221 gtcattccta cgaaagcggg aatccagtgt gttcggtttc agttgttttt gggtttcggg
     2281 tgatttccaa atcgaaacct ttgcaaaatt ccccaaaatc ccctaaattc ccaccaagac
     2341 atttagggga tttctcatga gcaccttctt ccaacaaacc gcccaagcca tgattgccaa
//
LOCUS       DAXHSF010000208         2356 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.208, whole genome shotgun sequence.
ACCESSION   DAXHSF010000208 DAXHSF010000000
VERSION     DAXHSF010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.208"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            267..773
                     /gene="lspA"
                     /locus_tag="ACFM8S_001946"
     CDS             267..773
                     /gene="lspA"
                     /locus_tag="ACFM8S_001946"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFC6314957.1"
                     /translation="MSSSVSSKTRYWVLALAAIVLDQWSKWAVLSSFQYRERVNVIPS
                     FFDLTLVYNPGAAFSFLADQGGWQKYFFLVLAVAVSAYLVRAILRDEFATLGKIGAAM
                     IIGGALGNVADRLIHGHVVDFLLFYWQNWFYPAFNIADSFICVGAVLAVLDNIVHRKD
                     SKKNVNAV"
     gene            792..1760
                     /gene="ispH"
                     /locus_tag="ACFM8S_001947"
                     /gene_synonym="lytB"
     CDS             792..1760
                     /gene="ispH"
                     /locus_tag="ACFM8S_001947"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFC6314958.1"
                     /translation="MNGKTIILANPRGFCAGVDRAISIVERALEEFGAPIYVRHEVVH
                     NKFVVDNLREKGAVFIEDLAEVPPGATLVYSAHGVSKAVRQEAAERGFRVFDATCPLV
                     TKVHKEVARLDAQDCEIIMIGHKGHVEVEGTMGQLAPGKMLLVETVGDVAKLEVRNPD
                     KLAYVSQTTLSVDETKDIIAALNARFPNIRNPHKEDICYATTNRQTAVKELAEQCDIV
                     IVVGSPNSSNSNRLREVAASRGIDAYMVDNAGYLQRAWFEGKNKVGVTAGASAPEVLV
                     REVLATIRGWGHETVREGEGAEESIVFVLPKELRREGETKPDLCKR"
     gene            complement(1847..>2356)
                     /locus_tag="ACFM8S_001948"
     CDS             complement(1847..>2356)
                     /locus_tag="ACFM8S_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFC6314959.1"
                     /translation="LPEIIRDLLEMPSETAIADITRTYSAHYLNPNNRNMTLFPDALP
                     CLDKLKAQGYWLAVATGKGRAGLDNAISQTATGGYWLATACAGEYPSKPSPEMVFGIC
                     GELGLDPKEALVVGDTAHDLHMAANAGAAAVGVTTGAHSREQLRTAPHLAVLDGLSEL
                     PGFLAQHYA"
BASE COUNT          511 a          574 c          737 g          534 t
ORIGIN      
        1 tttcgagatt gcggtgttgt tggaacgcaa ctgaaccgtc attcccacga aagtgggaat
       61 ctagaacgag gggtttgggc aactgttttt atccgataag tttctgtgcg gacaggtctg
      121 gattaccgcc tgcgcgggaa tgacgggttt cgagattacg gtgttgtcgg gaatgacggg
      181 ttttaagatt acggcatttg ccgtttcggg tacaggcagg gggttttcgg gtaaaatggc
      241 actcttttat ccggctgttg aaaaatatgt cttcatctgt ttcaagtaaa acgcgctatt
      301 gggtattggc acttgccgcc atcgtgctgg accagtggtc gaagtgggcg gtgctgtcgt
      361 cgtttcagta tcgggaacgc gtcaatgtca ttccttcgtt tttcgatctg accttggtgt
      421 acaacccggg cgcggcgttc agcttccttg ccgatcaggg cggctggcaa aaatactttt
      481 ttttggtgct ggcggtggcg gtgagcgcgt atttggtacg cgccattttg cgcgatgagt
      541 ttgcaaccct cggcaaaatc ggggcggcga tgattatcgg cggtgcgttg ggcaacgttg
      601 ccgaccgcct gatacacggt catgtcgtcg atttcttatt gttttattgg caaaattggt
      661 tttatcccgc ctttaatatt gccgacagct ttatctgcgt cggcgcggtg ttggcagtgt
      721 tggacaacat cgtccaccgc aaagatagca aaaaaaacgt gaatgccgtc tgaacacgga
      781 atgcaaaact tatgaacgga aaaaccatca tccttgccaa tccgcgcggc ttctgcgccg
      841 gtgtggatcg ggcaatcagt attgtcgaac gtgctttgga agaattcggc gcgccgattt
      901 atgtgcgcca cgaagtcgtc cacaacaaat tcgtcgtgga caacctgcgt gaaaaaggtg
      961 cggtgtttat tgaagacttg gcggaagtgc cgccgggcgc gacactggtt tattcggcac
     1021 acggcgtatc gaaggcggtg cggcaagaag cggcggagcg cggtttccgc gtgtttgatg
     1081 cgacttgccc gctggtgacg aaagtgcata aggaagtcgc ccgactggat gcccaagact
     1141 gtgaaatcat catgatcggg cataaggggc acgtcgaggt tgaaggaacg atggggcagc
     1201 ttgcgccggg caaaatgctt ttggtcgaaa cggtcggaga tgtggcaaaa ctcgaagtca
     1261 gaaaccccga caaactcgcc tatgtcagcc aaaccacgct ctcggtcgat gaaaccaaag
     1321 acatcatcgc cgcgctgaac gcgcgtttcc ccaatatccg caatccgcac aaggaagata
     1381 tctgctatgc gacgaccaac cggcaaaccg ccgtcaaaga gttggcagaa cagtgcgaca
     1441 tcgtgattgt ggtcggttcg cccaattcgt ctaacagcaa ccgcttgcgc gaagtggcgg
     1501 catcgcgcgg aatcgatgcg tatatggtgg ataatgcagg ctacctgcaa cgcgcgtggt
     1561 ttgagggcaa aaacaaagtc ggcgtaacgg caggcgcgtc cgcgcccgaa gtgttggtgc
     1621 gggaagtact ggcaaccata cgcggatggg ggcacgaaac cgtacgcgaa ggcgagggcg
     1681 cggaagaaag cattgtgttc gtcctgccca aagagctgcg ccgcgagggc gaaaccaaac
     1741 ccgatttgtg caaacgttga gcaagcgttg aatgtttggg caaaacaaat gccgtctgaa
     1801 caggcttcag acggcatttt tgccgcgtgc cggatgcgga agccaatcag gcgtaatgtt
     1861 gtgcaagaaa accgggcagt tcggacaaac cgtccaatac ggcgagatgc ggtgcggtgc
     1921 ggagctgttc gcgcgaatgt gcgccggtgg tcacgccgac tgccgccgcc cccgcgtttg
     1981 ccgccatatg caggtcgtgc gccgtatcgc cgacgaccaa tgcttctttc ggatcgagtc
     2041 ccagttcgcc gcagattccg aataccattt cgggcgaggg tttggaggga tattcccccg
     2101 cgcaggcggt ggcgagccaa tagccgccgg tggcggtttg gctgatggcg ttgtccaagc
     2161 ccgcccgccc tttgcccgtg gcgacggcaa gccagtatcc ttgtgctttg agcttgtcca
     2221 gacagggcag ggcatcggga aagagcgtca tattgcggtt gttgggattg aggtaatgtg
     2281 cggaataagt gcgtgtgatg tcggcaatgg cggtttcaga cggcatttcg agcaggtcgc
     2341 ggatgatttc gggcag
//
LOCUS       DAXHSF010000209         2339 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.209, whole genome shotgun sequence.
ACCESSION   DAXHSF010000209 DAXHSF010000000
VERSION     DAXHSF010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.209"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            44..2257
                     /locus_tag="ACFM8S_001949"
     CDS             44..2257
                     /locus_tag="ACFM8S_001949"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFC6314960.1"
                     /translation="MTAISPIQDTQSATLQELREWFDSYCAALPNNDKKLVLAARSLA
                     EAHYPADAATPYGEPLPDHFLGAAQMVHELDLLPDAVAATLLADIGRYVPDWNLLVSE
                     RCNSTVAELVKGVDEVQKLTHFARVDSLATPEERAQQAETMRKMLLAMVTDIRVVLIK
                     LAMRTRTLQFLSNAPDSPEKRAVAKETLDIFAPLANRLGVWQLKWQLEDLGFRHQEPE
                     KYREIALLLDEKRTERLEYIENFLNILRTELKKYNIHFEVAGRPKHIYSIYKKMVKKK
                     LSFDGLFDIRAVRILIDTVPECYTTLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTV
                     IVGPEDKGVEVQIRTFDMHQFNEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWREN
                     MAESGKEDLAAAFKTELFNDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGA
                     KVEGQIVPLSTPLENGQRVEIITAKEGHPSVNWLYEGWVKSNKAIGKIRAYIRQQNAD
                     TVREEGRVQLDKQLAKLTPKPNLQELAENLGYKKPEDLYTAVGQGEISNRAIQKACGT
                     LNEPPPVPVSETTIVKQSKIKKGGKNGVLIDGEDGLMTTLAKCCKPAPPDDIVGFVTR
                     DRGISVHRKTCPSFRHLAEQAPEKVLDASWAALQEGQVFAVDIEIRAQDRSGLLRDVS
                     DALARHKLNVTAVQTQSRDLEASMRFTLEVKQVTDLPRVLASLGDVKGVLSVTRL"
BASE COUNT          601 a          770 c          561 g          407 t
ORIGIN      
        1 ctgttacaat aaactcgttt ttatcggaac ggaagacccc atcatgaccg ccatcagccc
       61 gattcaagac acgcaaagcg cgactctgca agaattgcgc gaatggttcg acagctactg
      121 cgccgcgctg ccgaacaacg ataaaaaact tgtcttagcc gcccgttcgc tggcggaagc
      181 acattacccc gccgatgccg ccacgccgta tggcgagccg ctgcccgacc acttcctcgg
      241 cgcggcgcaa atggttcatg aactcgacct gctccccgat gccgtcgccg ccaccctgct
      301 tgccgacatc ggacgctacg tccccgattg gaacctattg gtttccgaac gctgcaacag
      361 taccgtcgcc gagctggtca aaggtgtgga cgaagtgcag aaactcaccc acttcgcccg
      421 ggtggacagc ctcgccacgc cagaagaacg cgcccagcag gcagaaacta tgcggaaaat
      481 gctgctggcg atggttaccg acatccgcgt cgtgttaatc aaactggcga tgcgtacgcg
      541 caccctgcaa tttttaagca acgcccccga cagccccgaa aaacgcgccg tcgccaaaga
      601 aaccctcgac atcttcgccc cgctcgccaa ccgtttgggc gtgtggcagc tcaaatggca
      661 gctcgaagat ttgggcttcc gccatcaaga acccgaaaaa taccgcgaaa ttgccctgct
      721 tttggacgaa aaacgcaccg aacgcctcga atacatcgaa aacttcctca atatcctgcg
      781 tacggaactc aaaaaataca atatccactt tgaagtcgcc ggccgtccga aacacatcta
      841 ctccatttac aaaaaaatgg tgaagaaaaa actcagcttc gacggcctct ttgacatccg
      901 cgccgtgcgg attctgattg ataccgtccc cgagtgttac accacgctgg gcatcgtcca
      961 cagcctctgg cagcccattc ccggcgagtt cgacgactac atcgccaacc ccaaaggcaa
     1021 cggctataaa agtttgcaca ccgtcatcgt cggcccggaa gacaaaggcg tggaagtgca
     1081 aatccgcacc ttcgatatgc accaattcaa cgaattcggt gtcgccgccc actggcgtta
     1141 caaagagggc ggcaaaggcg attccgccta cgaacagaaa atcgcctggt tgcgccaact
     1201 cttggactgg cgcgaaaaca tggcggaaag cggcaaggaa gacctcgccg ccgccttcaa
     1261 aaccgagctt ttcaacgaca cgatttatgt cctgaccccg cacggcaaag tcctctccct
     1321 gcccacgggc gcgaccccta tcgacttcgc ctacgccctg cacagcagca tcggcgaccg
     1381 ttgccgcggt gcgaaagtcg aagggcagat tgtgccgctg tccaccccgc tcgaaaacgg
     1441 acagcgcgtc gaaatcatta ccgccaaaga agggcatcct tccgtcaact ggctttacga
     1501 aggctgggtc aaatccaaca aggcaatcgg caaaatccgc gcctacatcc gccagcaaaa
     1561 cgccgacacc gtgcgcgaag aaggccgcgt ccaactcgac aaacagcttg ccaaactcac
     1621 gcccaaaccc aacctgcaag agcttgccga aaatctcggc tacaaaaagc cagaagacct
     1681 ctacaccgcc gtcggacaag gcgaaatttc caaccgcgcc atccaaaaag cctgcggcac
     1741 gctgaacgaa ccgccgcccg tacccgtcag cgaaaccacc atcgtcaaac agtccaaaat
     1801 caaaaaaggc ggcaaaaacg gcgtgctcat cgacggcgaa gacggtctga tgaccacgct
     1861 tgccaaatgc tgcaaacccg cgccgcccga cgacattgtc ggcttcgtta cccgcgatcg
     1921 cggcatttcg gtacaccgca aaacctgccc ctctttccga cacctcgccg aacaagcacc
     1981 tgaaaaagtg ctggacgcaa gctgggcggc gttgcaggaa ggacaagtgt tcgccgtcga
     2041 tatcgaaatc cgcgcccaag accgctccgg gcttttgcgc gacgtatccg acgcactcgc
     2101 ccgccacaaa ctcaacgtta ccgccgtgca aacccagtcc cgcgacttgg aagccagcat
     2161 gaggttcacg ctcgaagtca aacaagttac cgacctccca cgcgtcctcg ccagcctcgg
     2221 cgacgtcaaa ggcgtattga gcgttacccg gctttaaata caaaaatgcc gtctgaaagc
     2281 cgaataacgc ttcagacggc attttgattg ccggggtttg ctattttttg ttgcatagt
//
LOCUS       DAXHSF010000210         2318 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.210, whole genome shotgun sequence.
ACCESSION   DAXHSF010000210 DAXHSF010000000
VERSION     DAXHSF010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.210"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(53..1462)
                     /gene="dacB"
                     /locus_tag="ACFM8S_001950"
     CDS             complement(53..1462)
                     /gene="dacB"
                     /locus_tag="ACFM8S_001950"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HFC6314961.1"
                     /translation="MNFPKTAASLLLLLASLAAHALDTGRIPQNEIAVYVQELDSGKV
                     IIDHRSDVPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTVNDGTLDGNLYWAGS
                     GDPVFNQENLLAVQRQLREQGIRNITGHLMLDHSLWGEVGSPDHFEADSGSPFMTPPN
                     PTMLSAGMVMVRAERNAAGSTDILTDPPLPHIFAQNNLKITASQAACLSVKKLMRASF
                     SDNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNHWLLGGGRISDGIGIADTPEG
                     AQTLAVAHSKPMKEILTDMNKRSDNLIARSVFLKLGGNGKLPAVSEQAASAVRRELAV
                     SGIDVADLVLENGSGLSRKERVTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRN
                     RFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMVVVVIINSGRAVSLLPDLDNFVANNI
                     ISGGDGWLDAKLMCKERRA"
     gene            1627..2193
                     /locus_tag="ACFM8S_001951"
     CDS             1627..2193
                     /locus_tag="ACFM8S_001951"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFC6314962.1"
                     /translation="MAKKISILVGSLRRASFARKVALNAAEMFPEGWQAEIVEIGHLP
                     LYNFDYDDPAVEDVPLPESYTAFRETIKASDGILFVTSENNRTIPACLKNAVDIGSKP
                     NADVAWKNKPAGIISHSVGKMGGYSSQKNLRLALSYFDMPVTGQPEVFLGNSPTLFDE
                     NGKLIDSARDFVQSYINQFVGLIERNAK"
BASE COUNT          528 a          567 c          668 g          555 t
ORIGIN      
        1 tcctagttat ctaggacagc cccttaaatt taatccacta tattttcctg tttcaggctc
       61 ggcgttcttt gcacatcagt ttcgcatcca gccagccatc gccgccggag atgatgttgt
      121 tggcaacgaa gttgtccaag tcgggcagca gggaaacggc gcgaccgctg ttgatgatga
      181 cgaccaccac catcggtttg tcgcccagcc agtagcctgc caatgcgcgg acattgttga
      241 gtgtgcctgt tttcaggcgc agaagcccac cgctttgttt gaagcggttg cgtaaagttc
      301 cgtctgtgcc ggcgatgggc agcgtgtcga tgaaatcttg tgcaaacggg ctgaaataag
      361 ccgtttccaa catttgcgcc atcattctcg ccgttaccct ttcttttcta gacagacctg
      421 aaccgttttc caaaaccaaa tccgcaacat cgatgcccga cacggcaagt tcgcgccgga
      481 cggcagatgc cgcctgttcg gaaacggcgg gcagtttgcc gttgccgccg agtttgagga
      541 agacggaacg cgcaatcaga ttgtccgaac gcttgttcat gtccgtcaaa atttccttca
      601 tcggctttga gtgtgcaacg gcaagcgtct gcgcgccttc cggcgtgtcg gctatgccga
      661 tgccgtctga aatccgtccg ccgccgagca gccagtggtt ggtaaaactt tgccggatca
      721 gttcgtcaag cgcgaacatc cggacaccga caggcttgcc caaacagctc tcgggaatat
      781 tgccgcgcaa tttcagcgta ttgtccgaaa aagatgcacg catcagtttt ttgaccgaaa
      841 ggcaggcggc ttgggaggcg gtaattttca agttgttttg ggcgaaaata tgcggcaaag
      901 gcggatcggt gaggatgtcg gtactgccgg cggcattgcg ttcggcgcgc accataacca
      961 taccggcaga cagcatagtc ggattggggg gggtcataaa cggcgaaccg ctgtcggctt
     1021 caaaatggtc gggactgccg acttcgcccc acaggctgtg gtcgagcatc aggtgtcccg
     1081 tgatattgcg tatgccttgt tcgcgcaact ggcgttggac ggcaagcagg ttttcctgat
     1141 tgaaaacggg gtcgccgctg cccgcccaat acaggtttcc gtcaagcgtg ccgtcgttta
     1201 ccgtaccgtt gcttttaaac tcggtcgccc agcggtaatt gctgccgaag gttttgaagg
     1261 cggcaaacgc ggtaacgagt ttcattgtgg aggcggggtt gacggggaca tccgagcggt
     1321 ggtcaatgat gacttttccg ctgtcaagct cttggacata tacggcgatt tcgttttgcg
     1381 gaatgcggcc ggtatcgagc gcgtgtgcgg cgagggaggc gagaagcagc agcagggagg
     1441 ccgctgtttt ggggaaattc ataggtaaat ctgttacata aaaaaagcaa ttataaggca
     1501 aagccggata agtgggaacg ctgtggcggc gggccggctt gtttgcaggg gaaatcacat
     1561 atataataat cgctatcatt atgaaatgat tgagacgcac aaacttaata ttcaggagga
     1621 atgattatgg ctaagaaaat cagtattttg gtgggcagcc tgcgccgtgc ttcttttgcg
     1681 cgcaaagtgg cattgaatgc ggcggagatg ttccccgaag gctggcaggc ggaaatcgtc
     1741 gaaatcggac atctgccgct ttacaatttc gattatgacg accctgcggt ggaagatgtg
     1801 ccgctgcccg aaagctacac ggctttccgc gaaacgatta aggcttcgga cggcattttg
     1861 ttcgttacgt ccgaaaataa ccgcaccatt cccgcctgtt tgaaaaatgc ggtggacatc
     1921 ggctcgaaac cgaatgccga cgtggcgtgg aaaaacaaac cggccggtat catcagccat
     1981 tccgtcggca agatgggcgg ttacagttcg caaaaaaacc tgcgccttgc gctgtcttat
     2041 ttcgatatgc ccgtaaccgg acagccggaa gtgtttttgg gcaattcgcc gacgctgttt
     2101 gatgaaaacg gcaaattgat cgactcggca agggattttg ttcagtcata tattaatcag
     2161 tttgtcggtt tgattgaaag aaatgccaaa taaacaaacc aagtcgaaag gccgcaaaac
     2221 cggttcgggt tttgcggctt ttttatatgg gctgggcagc gtcaaggttt gccgtttgcc
     2281 ggataatgcg cggcaaatga ccggcgtgtt tatagcgg
//
LOCUS       DAXHSF010000211         2313 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.211, whole genome shotgun sequence.
ACCESSION   DAXHSF010000211 DAXHSF010000000
VERSION     DAXHSF010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.211"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..1005
                     /gene="rfbB"
                     /locus_tag="ACFM8S_001952"
     CDS             <1..1005
                     /gene="rfbB"
                     /locus_tag="ACFM8S_001952"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFC6314963.1"
                     /translation="IRNTQDSVVNLDKLTYAGNLESLTDIADNPRYAFEQVDICDRAE
                     LDRVFAQHRPDAVMHLAAESHVDRSIGSAGEFIQTNIVGTFNLLEAARAYWQQMPSEQ
                     HEAFRFHHISTDEVYGDLGGTDDLFTETAPYAPSSPYSASKASSDHLVRAWLRTYGLP
                     TIVTNCSNNYGPYHFPEKLIPLMILNALDGKPLPVYGDGMQIRDWLFVEDHARALYQV
                     VTEGEIGETYNIGGHNEKANIEVVRTICALLEELVPNKPQGVARYEDLITYVKDRPGH
                     DVRYAIDAAKIGRELGWQPQETFESGIRKTVEWYLDNKTWWQNVLNGSYRLERLGTGK
                     "
     gene            1068..1934
                     /gene="rfbA"
                     /locus_tag="ACFM8S_001953"
     CDS             1068..1934
                     /gene="rfbA"
                     /locus_tag="ACFM8S_001953"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFC6314964.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILVITAPEDNASFKRLLGDGSDFGISISYAVQPSPDGLAQAFIIGEEFIGNDNV
                     CLVLGDNIFYGQSFTQTLRQAAAKKHGATVFAYQVKNPERFGVVEFDANFRALSVEEK
                     PQQPKSNWAVTGLYFYDNRAVEFAKRLKPSARGELEISDLNQMYLEDGSLSVQILGRG
                     FAWLDTGTHESLHEAASFVQTVQNIQNLHIACLEEIAWRNGWLSDEKLEELARPMAKN
                     QYGQYLLRLLKK"
     gene            1973..>2313
                     /gene="rfbC"
                     /locus_tag="ACFM8S_001954"
     CDS             1973..>2313
                     /gene="rfbC"
                     /locus_tag="ACFM8S_001954"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFC6314965.1"
                     /translation="MKDKKMNIIDTAIPDVKLLEPQVFGDARGFFMETFRDEWFKTQV
                     CERTFVQENHSKSGKGVLRGLHYQTENTQGKLVRVVVGEVFDVAIDMREGSPTFGKWA
                     GATLSAQNQCQL"
BASE COUNT          597 a          633 c          578 g          505 t
ORIGIN      
        1 atccgaaaca cccaagattc cgtcgtcaac ctcgacaaac tgacctacgc gggcaatctc
       61 gaatcgctga ccgacattgc cgataatccc cgctatgctt tcgagcaagt ggacatttgc
      121 gaccgcgccg aactcgaccg cgtattcgca caacaccggc ccgatgccgt gatgcacttg
      181 gcggcggaaa gccatgtcga ccgctctatc ggttcggcag gcgagtttat ccaaaccaat
      241 atcgtcggca cattcaatct gcttgaagca gcccgcgcct actggcaaca aatgccgtct
      301 gaacagcacg aagccttccg tttccaccat atttccaccg atgaagtcta tggcgattta
      361 ggcggcacgg acgatttgtt taccgaaacc gcgccctacg cgccgtccag cccctactct
      421 gcctctaaag cgtccagcga ccacctcgtc cgcgcgtggt tgcgtactta cggcctgccg
      481 accattgtaa ccaattgttc caacaactac ggcccttatc attttcctga aaaactgatt
      541 ccactgatga ttctgaatgc gcttgatggc aaaccgctgc cggtgtatgg cgacggtatg
      601 cagattcgtg attggttgtt tgtcgaagat catgcgcgtg cgctgtatca agtggtcaca
      661 gaaggcgaaa tcggcgaaac ttacaacatt ggtggccaca atgaaaaagc caatatcgaa
      721 gtggtgcgca ctatttgcgc actattggaa gaattggtgc caaacaaacc gcaaggcgtg
      781 gcgcgttatg aagatttgat tacttatgtg aaagaccgcc ccggccacga tgtgcgctat
      841 gcgattgatg cagcaaaaat cggccgcgag ttgggttggc agccgcagga aacttttgaa
      901 agcggcattc gcaaaacagt ggaatggtat ctggacaaca aaacctggtg gcaaaatgta
      961 ttgaacggca gctatcgttt ggaacgttta ggtactggaa aatagttttc agacggcatc
     1021 ccgacgcaat gccgtctgaa aacccatcgc aaaggaagaa agaaaagatg aaaggcatca
     1081 tactggcagg cggcagcggc acgcgcctct accccatcac gcgcggcgta tccaaacagc
     1141 tcctgcccgt gtacgacaaa ccgatgattt attacccctt gtcggttttg atgctggcgg
     1201 gaatccgcga tattttggtg attaccgcgc ctgaagacaa cgcctctttc aaacgcctgc
     1261 ttggcgacgg cagcgatttc ggcatttcca tcagttatgc cgtgcaaccc agtccggacg
     1321 gcttggcaca ggcatttatc atcggcgaag aatttatcgg caacgacaat gtttgcttgg
     1381 ttttgggcga caatattttt tacggtcagt cgtttacgca aacattgcgg caagcggcgg
     1441 caaaaaaaca cggcgcgacc gtgtttgctt atcaggtcaa aaatcccgaa cgtttcggcg
     1501 tggttgaatt tgacgcaaac ttccgcgccc tgtctgtcga agagaaaccg caacagccca
     1561 aatccaattg ggcggtaacc ggcttgtact tctacgacaa ccgtgccgtc gagttcgcca
     1621 aacggctcaa accgtccgca cgcggcgaat tggaaatttc cgacctcaac caaatgtatt
     1681 tggaagacgg ttcattgtcc gtccaaatat taggacgcgg tttcgcatgg ttggacaccg
     1741 gcacccacga gagcctgcac gaagccgctt cattcgtcca aaccgtgcaa aacatccaaa
     1801 acctgcacat cgcctgcctg gaagaaatcg cttggcgcaa cggttggctt tccgatgaaa
     1861 aactggaaga attggcgcgc ccgatggcga aaaaccaata cggccaatat ttgctgcgcc
     1921 tgttgaaaaa ataatgtttg aggccgtctg aaacttttca gacggccttt agatgaaaga
     1981 taaaaagatg aacatcattg ataccgccat tcctgacgta aaactgcttg agccccaagt
     2041 cttcggcgac gcgcgcggct tttttatgga aaccttccgc gacgagtggt ttaaaaccca
     2101 agtctgcgaa cgcaccttcg tgcaggaaaa ccactccaaa tccggcaaag gcgtattgcg
     2161 cggcctgcac taccaaaccg aaaacacaca aggcaaactc gtacgcgtag ttgtcggcga
     2221 agtgttcgac gtggccatcg atatgcgcga aggttcgccg actttcggca aatgggcggg
     2281 cgcaaccctg tccgcgcaaa accaatgcca gct
//
LOCUS       DAXHSF010000212         2287 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.212, whole genome shotgun sequence.
ACCESSION   DAXHSF010000212 DAXHSF010000000
VERSION     DAXHSF010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.212"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            20..748
                     /gene="rpe"
                     /locus_tag="ACFM8S_001955"
     CDS             20..748
                     /gene="rpe"
                     /locus_tag="ACFM8S_001955"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFC6314966.1"
                     /translation="MTTYRIAPSILSADFARLGEEVESVIAAGADLIHFDVMDNHYVP
                     NLTFGPMVCAALKPYASVPIDVHLMVEPVDDLIQSFAKAGASIITFHPEASRHIDRSL
                     SLIRDMGCQAGLVLNPATPVYLLENVLDRLDMVLLMSVNPGFGGQSFIPHTLEKIRRV
                     RAMLDRYEAQSGRHIAIEVDGGIKTDNIAAVARAGADTFVAGSAIFGKPDYKAVIDAM
                     RAELEKSGRLNLAPMIEIEMPSEN"
     gene            complement(771..1802)
                     /gene="ruvB"
                     /locus_tag="ACFM8S_001956"
     CDS             complement(771..1802)
                     /gene="ruvB"
                     /locus_tag="ACFM8S_001956"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFC6314967.1"
                     /translation="MLQTDNLTAAQPQRIVAAQTASAQEELLERALRPKTLDDYIGQH
                     KAKEQLAIFIQAAKKRGEALDHVLLFGPPGLGKTTLAHIIAKELGVNLRQTSGPVLER
                     AGDLAALLTNLDPHDVLFIDEIHRLSPVVEEILYPALEDYRLDIMIGEGPAARSVKID
                     LPPFTLVGATTRAGMLTNPLRDRFGIVSRLEFYENRDLATIVSRSAQLLQLDMSEEGA
                     EEIAKRSRGTPRIANRLLRRVRDFADVKNNGTIDGGIADAALSMLDVDVQGLDVMDRK
                     FLEAVLHKFGGGPVGLDNVAAAIGESTDTIEDVIEPYLIQQGFLQRTPRGRMATERAY
                     LHFGLPVEK"
     gene            complement(1884..2192)
                     /locus_tag="ACFM8S_001957"
     CDS             complement(1884..2192)
                     /locus_tag="ACFM8S_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314968.1"
                     /translation="MTLKTDLLPKINNEDYQRLILRHSAEFSGGEIRLLNEILEKFNF
                     DVVQAQALAQAVMQQVRFDPNAYHIDSDDEDTTGICPHCINPPMPPLRDYLVWRETRG
                     "
BASE COUNT          463 a          550 c          733 g          541 t
ORIGIN      
        1 gatattgaag gaaaaaaaca tgactactta ccgtatcgca cccagcattt tatcggcaga
       61 ttttgcacgg cttggcgaag aggtggaaag cgtcattgcg gcaggtgcag atctgatcca
      121 ttttgacgtg atggacaacc attatgtgcc gaacctgacc ttcggcccta tggtttgcgc
      181 ggcgttgaag ccttatgcaa gcgtgccgat cgatgtgcat ctgatggtcg aacccgttga
      241 cgacctgatt cagtcgtttg ccaaagcagg agcatcaatc atcacgttcc atcccgaagc
      301 gagccgccat atcgaccgca gcttgagcct gattcgggac atgggctgtc aggcggggct
      361 ggtgttgaat ccggcaacgc ccgtatatct gttggaaaac gtattggaca ggctggatat
      421 ggttttgctg atgtcggtca accccggatt cggcggacaa agcttcatcc cgcacaccct
      481 tgaaaaaatc cgccgggtgc gggcgatgct ggatcggtac gaagcacaaa gcgggcggca
      541 catcgccatc gaagtggacg gcggcatcaa aaccgacaat attgccgccg ttgcccgagc
      601 tggtgcggat acctttgttg ccggttctgc gattttcggc aaaccagact ataaggcggt
      661 gattgatgcg atgagggcgg aactggaaaa aagcggcagg ctgaaccttg cgccaatgat
      721 tgaaatagaa atgccgtctg aaaattagct ctgtttcaga cggcattgcg ttatttttcg
      781 acgggcagcc cgaaatgcag gtaggcgcgt tcggtcgcca tcctgccgcg cggggtgcgt
      841 tgcaggaagc cttgttggat gagatagggt tcgataacgt cttcgatggt gtctgttgat
      901 tcgccgatgg cggcggcaac attgtccaaa ccgaccgggc cgccgccgaa tttgtgcaaa
      961 acggcttcga ggaatttcct gtccatcacg tccagcccct gcacgtccac gtccagcata
     1021 cttaaagcgg catcggcgat gccgccgtcg attgtgccgt tgtttttcac gtcggcgaaa
     1081 tcgcgcacgc ggcgcaacag gcggttggcg atgcgcggcg taccgcggct gcgtttggcg
     1141 atttcttccg cgccttcttc ggacatatcg agttgcagga gttgtgccga acggctgacg
     1201 atggtggcaa ggtcgcggtt ttcgtaaaac tcgaggcggg agacgatgcc gaagcggtcg
     1261 cgcaacggat tggtcagcat accggcgcgg gtggtcgcgc cgacaagcgt gaagggcggc
     1321 aggtcgattt taacggaacg ggcggcgggc ccttcgccta tcataatgtc gagccggtag
     1381 tcttcaagcg cggggtagag gatttcttcg acaacagggc tgaggcggtg gatttcgtcg
     1441 ataaacaata cgtcgtgcgg atcaaggttg gtcaaaaggg cggcgagatc gccggcgcgt
     1501 tcgaggacgg ggccgctggt ttggcgcaaa tttacgccca attctttggc gatgatgtgc
     1561 gccagtgtgg ttttacccag tccgggcggg ccgaagagca aaacgtggtc gagtgcttcg
     1621 ccgcgttttt tggcggcttg gatgaaaatg gcgagctgtt ctttggcttt atgctgcccg
     1681 atatagtcgt ccagcgtttt ggggcggagg gcgcgttcga gcagttcttc ctgtgcggag
     1741 gcggtttggg cggcaacaat gcgttgcggt tgagcggcag tcaggttgtc ggtttgcagc
     1801 atagtgttcc ctttgtcggg tatgccgtct gaacggtcgg cggcgtttca gacggcattg
     1861 aaaagataac ggtcaaaagc gttttacccg cgcgtttcgc gccaaacgag atagtcgcgc
     1921 aggggcggca taggcgggtt gatgcagtgt gggcagatgc ctgtggtgtc ttcgtcgtcg
     1981 ctgtcgatgt gataggcgtt ggggtcgaag cggacttgct gcatcaccgc ctgcgccaac
     2041 gcctgcgcct gaacaacgtc gaaattgaat ttttcgagga tttcgttcaa taggcggatt
     2101 tcgccaccgc taaattccgc actgtgtctg aggatgaggc gttgataatc ttcgttgttg
     2161 attttaggca ataaatcggt tttcagggtc atgattttat gtcgggggcg gaatggctga
     2221 agcgtgaatt atagcggata tggcggcggc tgttgcaaag cttggattgg gtatcgtcct
     2281 caaaact
//
LOCUS       DAXHSF010000213         2261 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.213, whole genome shotgun sequence.
ACCESSION   DAXHSF010000213 DAXHSF010000000
VERSION     DAXHSF010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.213"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(<1..567)
                     /locus_tag="ACFM8S_001958"
     CDS             complement(<1..567)
                     /locus_tag="ACFM8S_001958"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFC6314969.1"
                     /translation="MEHKLPQLPYELDALSPHLSKETLEFHYGKHHQTYITNLNNQIK
                     GTEFENLPLEEIVKKSSGGVFNNAAQTWNHTFYWLGFTSKGQGKPAGELAAAIDAKWG
                     SFEKFQEAFNACAAGTFGSGWAWLVKTPAGGLDLVSTSNAATPLTTENTPLLTCDVWE
                     HAYYIDYRNSRPNYLKGFWEIVNWDEVAK"
     gene            730..2136
                     /gene="dnaB"
                     /locus_tag="ACFM8S_001959"
     CDS             730..2136
                     /gene="dnaB"
                     /locus_tag="ACFM8S_001959"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFC6314970.1"
                     /translation="MNDYAAMPSEDREVGALSLPPHSMEAEQSVLGGLMLENPAWDRI
                     ADVVSGEDFYRHEHRLIFRSIAKLINESRPADVITVQEDLQRNEELEAAGGFEYLITL
                     AQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGQLLDEAENKVFQ
                     IAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVPTGFIDLDKKTSGLQPG
                     DLIIVAGRPSMGKTAFSINIAEHVAVEGKLPVAVFSMEMGGAQLVMRMLGSVGRLDQS
                     VLKTGRLEDEHWGRLNEAVVKLSDAPVYIDETPGLTALELRARARRLARQFNNKLGLI
                     VIDYLQLMAGSGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVESRTDKRPM
                     MSDLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGKHRNGPVGKIFLTWTGQ
                     FTKFDNAAYIPEEAKIED"
BASE COUNT          547 a          589 c          604 g          521 t
ORIGIN      
        1 tttggcgact tcgtcccagt tgacgatttc ccaaaaacct ttcaggtagt tggggcggct
       61 gttgcggtag tcgatgtagt aggcgtgttc ccacacgtcg caggtcagca gcggcgtgtt
      121 ttcagtggtc agcggcgtag cggcgttgga agtagaaacc aaatccaatc cgccggcagg
      181 ggtttttacc agccacgccc aaccggagcc gaaagtaccg gccgcgcagg cattgaacgc
      241 ttcttggaat ttctcgaagc tgccccattt cgcgtcgatg gcggcggcca gttcgccggc
      301 aggtttgcct tgacctttgg acgtgaaacc cagccagtag aaggtgtggt tccaagtttg
      361 tgccgcgttg ttgaacacgc cgcccgaaga ttttttcaca atctcttcca aaggcaggtt
      421 ttcaaattcg gtgcctttga tttgattgtt caggttggtg atgtaggttt gatggtgttt
      481 gccgtagtgg aattccaaag tctctttgct cagatgcggg gacaatgcgt ccagttcata
      541 aggcagttgc ggcagcttat gttccatttt gtgctcctaa tattgtttta aatgttgtat
      601 tttggcagtg ttgctgcaaa tagctcagca gcgcacatat tttacctgtt ttgagggatg
      661 gaaaccaatt aaacctgctt tacgctataa tagaagattg caatttcggc acgacagata
      721 ggatgtacca tgaacgatta cgcagccatg ccgtctgaag accgtgaggt cggcgcattg
      781 tcgcttccgc cgcactcaat ggaggcggaa caatccgttt tgggcgggtt gatgctggaa
      841 aatccggcat gggacaggat tgccgatgtg gtttcgggag aggatttcta ccggcatgaa
      901 caccgcctga ttttccgttc cattgccaaa ttaatcaatg agagccgtcc cgcagatgtg
      961 attacggttc aggaagattt gcagcggaac gaagaattgg aagcggcagg cggattcgaa
     1021 tacctgatta cgctggcgca aaacaccccg tctgccgcca acatccgccg ctacgccgaa
     1081 atcgtgcgcg agcgttccat tatgcgccaa ctcgccgaag tggggacgga aatcgcccgc
     1141 agcgcctaca atccgcaagg cagggacgcg gggcagcttt tagacgaggc ggaaaacaaa
     1201 gtattccaaa tcgccgaaag caccgccaaa tccaagcagg gctttttgga gatgcccgat
     1261 ttgctgaaag aagtcgtaca gcgcatcgat atgctctact cgcgcgacaa tcccgatgaa
     1321 gttaccggcg tgccgacagg gttcatcgac ctcgacaaaa aaacctcggg tctgcaaccc
     1381 ggcgacctga ttatcgttgc cggtcgtccg tctatgggta agaccgcctt ttccatcaat
     1441 atcgccgaac acgttgccgt agaaggcaag ctgccggttg ccgtcttctc aatggagatg
     1501 ggcggtgcgc aactggtcat gcgtatgctc ggctcggtcg gacgcttgga tcaaagcgtc
     1561 ctgaaaaccg gcaggctcga agacgaacac tggggtcgtc tgaacgaagc agtcgtcaaa
     1621 ctctccgacg cgcccgtgta catcgacgag accccgggtt tgactgcgct cgaactgcgc
     1681 gcccgcgccc gccgtctcgc ccgtcaattt aacaataagc tgggattgat cgtcatcgac
     1741 tacctgcaac tgatggcggg atccggccgt tccgacaacc gagcttcaga gctgggcgaa
     1801 atttcacgtt cgctcaaagc gttggcgaaa gaattgcaag tccccatcat cgccctgtcg
     1861 caactgagcc gcacggtcga atcgcgtacc gacaaacgcc cgatgatgtc cgacctccgc
     1921 gaatccggtg ctatcgaaca agatgccgac ctgattatgt tcatgtaccg cgacgaatac
     1981 tacaaccagg actcacccat gaaaggcctt gccgaatgta tcatcggcaa acaccgcaac
     2041 ggtcccgtcg gtaaaatctt cctcacatgg acgggacaat tcaccaaatt cgacaatgct
     2101 gcctatattc ccgaggaggc aaagatagaa gattaaatgg ctatataaaa atttattagg
     2161 cgaaatcagg caaaatcgtt taaaatcatg ctgagagatt gccctaaaaa ataaaacgcg
     2221 gtcttgaggc atttttgcat tcagcccgca tataattgaa a
//
LOCUS       DAXHSF010000214         2255 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.214, whole genome shotgun sequence.
ACCESSION   DAXHSF010000214 DAXHSF010000000
VERSION     DAXHSF010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.214"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            111..794
                     /locus_tag="ACFM8S_001960"
     CDS             111..794
                     /locus_tag="ACFM8S_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HFC6314971.1"
                     /translation="MQHDVYDYTAHTVSKNTVLQKTYRLLGFSFIPAAAGAALAANAG
                     FNFYAAFGSRWIGFAVVLAFFYGMIHFIEKNRYSNTGVTLLMVFTFGMGVLIGPVLQY
                     ALHIADGAKIVGIAAAMTAAVFLTMSALARRTRLDMNALGRFLTVGAVILMVAVVANL
                     FLGIPALALTISAGFVLFSSLMIMWQVRTVIDGGEDSHISAALTLFISLYNIFSSLLN
                     ILLSLNGED"
     gene            918..1430
                     /gene="coaD"
                     /locus_tag="ACFM8S_001961"
     CDS             918..1430
                     /gene="coaD"
                     /locus_tag="ACFM8S_001961"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFC6314972.1"
                     /translation="MLPNTPRRAVYAGSFDPPTLGHLWMIRQAQSMFDELIVAIGINP
                     DKRSTYTVAERQDMLCDITKMFPNVRTDVFENRFLVHYAREVDAGFIVRGIRSASDYE
                     YERSMRHINSDLAPEISTVFLMPPREIAEVSSTMVKGLVGPEGWTETVKRYVPPAVYQ
                     KMIAEHHNNA"
     gene            1466..2119
                     /locus_tag="ACFM8S_001962"
     CDS             1466..2119
                     /locus_tag="ACFM8S_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HFC6314973.1"
                     /translation="MWEILFRHQDFVAINKPQGISVHRSDGEVSLTATLAAQLGVEKV
                     WLLHRLDKQTGGILLFALNSQSAAVLAAQFAERKMKKTYLALSDRKPSKKQGWIKGGM
                     EKSRRGMWKLTRNMENIAVTQFFSIRISEKMRLFILEPHTGKTHQLRVAMKSLGSPIL
                     GDRLYGGTESETTFLYAWKIQFTYQQQEIEITAPLKNAWQAENISHALEEFYMAKAV"
BASE COUNT          595 a          573 c          527 g          560 t
ORIGIN      
        1 cctttaaaat acgctggaca actttatctt cacgcaaccg gacaaatttt gacattgggc
       61 aatgttaaaa tcccccatta ttcccaaccg cgctttcagg agcagatgat atgcaacacg
      121 acgtttacga ctacaccgcg catacggttt ctaaaaacac cgtcctgcag aaaacctacc
      181 gcctgctcgg attttcattc attccggctg ccgcaggcgc ggcacttgcc gccaatgccg
      241 gtttcaattt ttacgccgcc ttcggttcgc gctggatagg gtttgccgtc gtgttggcgt
      301 ttttctacgg tatgatccac ttcatcgaga aaaaccgtta cagcaatacc ggcgttaccc
      361 tgctgatggt attcacattc ggtatgggcg tattgatcgg ccccgtgctg caatacgcgc
      421 tccatattgc cgacggtgcg aaaatcgtcg gcattgccgc cgcgatgacc gccgccgtct
      481 ttttaacgat gtccgctttg gcgcgccgaa cccggctcga tatgaacgcg ctcggacgct
      541 tcctgaccgt aggtgcggtc attctgatgg tcgccgtggt tgccaatctg tttttgggta
      601 ttcccgcact cgccctgacc atttccgccg gttttgtctt gttcagttcc ttaatgatta
      661 tgtggcaggt acgcaccgtc atcgacggcg gcgaagacag ccacatcagc gcggcactga
      721 cactgtttat ctcgctttac aacatcttca gcagcctgct gaacatcctg ttgtctttaa
      781 acggcgagga ttgatgccgg taaaacgccg tccgactatg ccgtctgaaa atgcttcaga
      841 cggcatttta ctttgggcat acaatacgga tgcacacgca ccaacacaca gccgactatg
      901 aagccgggag gaatcaaatg ttaccgaata caccgcgccg cgccgtttat gccggcagtt
      961 tcgatccgcc cacattgggg catctgtgga tgatacggca ggcgcaatct atgtttgacg
     1021 aactcatcgt cgccatcggc attaaccccg acaaacgcag cacctatacc gtcgctgaaa
     1081 ggcaggatat gttgtgcgat attactaaaa tgtttcccaa cgtcagaacc gatgtatttg
     1141 aaaaccgatt tctggtgcat tacgcccgtg aggtagatgc aggattcatc gtgcgcggca
     1201 tccgttctgc ttcggattac gaatacgaac gttccatgcg ccatatcaac agcgacctcg
     1261 cccccgaaat atccaccgta ttcctcatgc cgccgcgcga aatcgccgaa gtgtcgtcca
     1321 ctatggtcaa aggtttggtc gggccggaag gctggacgga aaccgtcaaa agatatgtgc
     1381 cgccggccgt gtaccaaaaa atgattgcag aacatcacaa caatgcctga cagaaagctt
     1441 ctgtcaaata tattcaggtt caaatatgtg ggaaatctta ttcagacacc aagattttgt
     1501 cgccatcaat aaacctcaag gcatatccgt ccaccgttcc gacggcgaag tcagtctgac
     1561 ggcaacactt gcggcacagt tgggcgtaga aaaggtatgg ttgctgcaca ggttggacaa
     1621 gcagaccgga ggcatattgc tgtttgccct taattcccaa agtgccgctg ttcttgccgc
     1681 acaatttgcc gaaaggaaaa tgaagaaaac ctatttagct ttgtccgacc gcaaaccgtc
     1741 caaaaaacaa ggatggataa aaggcggcat ggaaaaatcc agacgcggaa tgtggaagct
     1801 gacgcgcaat atggaaaata ttgccgttac ccagtttttc agtatacgta tcagtgaaaa
     1861 aatgcggctg ttcattcttg agcctcatac cggtaagaca catcagctga gagtggcaat
     1921 gaaaagtctg ggcagtccta ttttggggga ccgcttatac ggagggacag aatccgaaac
     1981 tacgttcctg tatgcatgga aaatacaatt tacctatcaa caacaagaaa tcgaaattac
     2041 tgcacctttg aaaaatgcat ggcaggctga aaatatctct catgcgctgg aagaatttta
     2101 tatggcaaaa gcagtttaat aaatataaat taatttataa acaatatgat aaatattttt
     2161 ataaaaattt tttaagaaaa cggttgaccg caaatctctt caagcgtata gttcgcttct
     2221 tcgctgctga cgaagcggcg gtgatgaagc agttc
//
LOCUS       DAXHSF010000215         2244 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.215, whole genome shotgun sequence.
ACCESSION   DAXHSF010000215 DAXHSF010000000
VERSION     DAXHSF010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.215"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(<1..281)
                     /locus_tag="ACFM8S_001963"
     CDS             complement(<1..281)
                     /locus_tag="ACFM8S_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314974.1"
                     /translation="MVTDKYKLSLEENIFLAKKVLVAQIHNLSRFENCQTTLLQTEQI
                     IHGKNVASVSLEDIQTILNLKRAYQYVISHISNGEPVNISLLKKINNIVA"
     gene            complement(406..939)
                     /locus_tag="ACFM8S_001964"
     CDS             complement(406..939)
                     /locus_tag="ACFM8S_001964"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HFC6314975.1"
                     /translation="MSPTHEISNVERLIDEINRIHAGYHERVSKLYGGNFPNLSKMLR
                     HLDIDFVLQYRLNLHESINDYLIRFDIGDLPLAYYYRVKTTESVFDKIRRFSEREPGY
                     PANKVLNDIFGVRIIADSALMDEIHAALDEWVSTKGLKNWYVQEKTAISERMCTSIKE
                     IICFFRGNFRYGIRRMP"
     gene            complement(1252..1524)
                     /locus_tag="ACFM8S_001965"
     CDS             complement(1252..1524)
                     /locus_tag="ACFM8S_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314976.1"
                     /translation="MNKRMKICPTCQQGYLYHSKPKYLHDEIILCDECDAVWLKGVNI
                     FYGEYKKDFYSYVPFMESQGITGECIWEGELFDHPYYEDENSNGMD"
     gene            complement(1711..2199)
                     /locus_tag="ACFM8S_001966"
     CDS             complement(1711..2199)
                     /locus_tag="ACFM8S_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314977.1"
                     /translation="MKKDIFYCEQWSYGYKRLHKPFSEKQAEEKHLKGELYSAVIGSA
                     TQPEYVITLREEVGFFSVHFFDKFGRDYLTHQFQKYSNSNYYFLSMAVWRDYITLDSH
                     DLAEGYTYFFNENTDDCYVLKEDFINNERYEKTELYSQKDKVILFPKFGEYDLVLNPD
                     II"
BASE COUNT          704 a          460 c          356 g          724 t
ORIGIN      
        1 gcaacaatgt tgttgatttt tttaaggagt gaaatattga ccggttcgcc atttgaaata
       61 tgcgaaatca catattgata ggcacgtttc aggttcaaga tggtttggat gtcttcaagt
      121 gacacggagg ctacattttt gccatggata atttgctcag tctgcaataa ggtcgtctga
      181 caattctcaa aacggctgag gttgtgaatt tgggcaacca atactttctt tgccagaaaa
      241 atattttctt ccaaactcaa cttgtatttg tccgtaacca cgagagttcc tttaataatt
      301 aaatctgtac acccaaggta tgcacggtaa acggtgctat attaaaaaga cccattaaag
      361 gttaaacaaa aagtcgcttg tcccgttcat ggctctcaat atttttcaag gcatccgcct
      421 tatcccatat ctgaagttcc cacggaaaaa acagattatt tctttgattg aagtacacat
      481 gcgttccgat atagccgttt tttcctgaac gtaccagttt tttaaaccct tcgtcgaaac
      541 ccattcatct aaagcagcat gtatttcgtc cattagcgcg gaatcagcga tgattctcac
      601 cccaaaaata tcatttaaca ccttattcgc cggatacccg ggctcgcgtt ccgaaaaccg
      661 gcggattttg tcgaaaacgg actccgtggt tttaacgcgg tagtagtatg ctaaaggcaa
      721 gtccccgatg tcaaatcgaa tcagatagtc gttgattgac tcatgcaaat taagtcggta
      781 ttgcaacaca aaatcgatgt ccagatgacg gagcatcttt gacaaattgg gaaagtttcc
      841 gccatacagt ttggaaaccc tttcgtgata tccggcgtgt atgcggttaa tttcgtcaat
      901 cagccgttct acattactga tttcatgagt gggggacatt ccttattact ctttcattcc
      961 aattatatga aaataacata ctaattgctt tcaactatat attatacaaa acaaatttca
     1021 aaacttcctt ttaatttttc attaccgtta atacgctaaa ataggaggaa ttgcaaatcc
     1081 aaaaaacata cacggtaaca gattacaggt catcaatgaa tccaatccat cccatgaaga
     1141 aaaaggagcg actactcggc ctccaagcag cctcccgtac tgaaaacgac attgcattaa
     1201 agacagcgcc gtttgatggc taatcgtacc tacgcaggct taaagtttcc atcaatccat
     1261 accatttgag ttttcatctt cataatatgg atgatcgaac agctctcctt cccagataca
     1321 ttcacccgtt ataccttggg attccatgaa aggaacataa gaataaaaat cttttttata
     1381 ttctccataa aatatattca ctcctttgag ccatactgca tcgcattcat cacacagaat
     1441 aatttcatca tgaagatatt taggtttcga atggtagaga tagccttgtt gacaagtggg
     1501 acatattttc attctcttat ttatgattat tccatataat caatttattt ttgtgggtaa
     1561 gcagtagtga tttgattgct gcctttagtc tgtacaacaa tacgtatata gcaatctgat
     1621 aatttcctgg tttttatcag ctttatcctc agattccaaa cccaataata aaagccgtct
     1681 gaaatttttt cagacggcct taaaacttaa ttaaataatg tccggattta acaccaaatc
     1741 atattcgcca aactttggaa atagaattac cttatctttt tgggaatata attctgtttt
     1801 ttcatatcgc tcattattaa taaaatcctc tttcaaaaca tagcaatcat ccgtattttc
     1861 attgaagaaa taagtatatc cttctgctaa gtcatgagag tctaaagtta tataatctct
     1921 ccatacagcc atagaaagaa aataataatt cgaattagaa tatttttgaa attgatgggt
     1981 taaataatcc cttccaaatt tatcgaaaaa atgtaccgaa aaaaaaccta cttcctctcg
     2041 caaggtaatt acatattcag gttgtgtcgc cgaacctatt acggcactat ataactcccc
     2101 tttaagatgt ttttcttcag cttgtttctc agaaaaaggc ttatgaagtc tcttataacc
     2161 ataagaccac tgctcacaat aaaaaatatc tttcttcatt tgtaaatcct tcctgttgta
     2221 tctctaatga taacattttc ttct
//
LOCUS       DAXHSF010000216         2216 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.216, whole genome shotgun sequence.
ACCESSION   DAXHSF010000216 DAXHSF010000000
VERSION     DAXHSF010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.216"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            99..1910
                     /gene="typA"
                     /locus_tag="ACFM8S_001967"
     CDS             99..1910
                     /gene="typA"
                     /locus_tag="ACFM8S_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFC6314978.1"
                     /translation="MKQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRANQQVDERVMDS
                     NDLEKERGITILAKNTAIDYEGYHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQE
                     GPMPQTRFVTKKALALGLKPIVVINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFP
                     IVYASGLSGFAKLEETDESNDMRPLFDTILKYTPAPSGSADETLQLQISQLDYDNYTG
                     RLGIGRILNGRIKPGQTVAVMNHDQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIIS
                     GIEDIGIGVTITDKDNPKGLPMLSVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDRL
                     QKELLTNVALRVEDTADADVFRVSGRGELHLTILLENMRREGYELAVGKPRVVYRDID
                     GQKCEPYENLTVDVPDDNQGAVMEELGRRRGELTNMESDGNGRTRLEYHIPARGLIGF
                     QGEFMTLTRGVGLMSHVFDDYAPVKPDMPGRHNGVLVSQEQGEAVAYALWNLEDRGRM
                     FVSPNDKIYEGMIIGIHSRDNDLVVNPLKGKKLTNIRASGTDEAVRLTTPIKLTLEGA
                     VEFIDDDELVEITPQSIRLRKRYLSELERRRHFKKLD"
BASE COUNT          564 a          632 c          579 g          441 t
ORIGIN      
        1 gcaagggctt cagacggcat cttcatttcc caaatactgt ccggtaaagc gtggtataat
       61 gcgcagttat ctcttacagt ttttgaaaaa cattaattat gaaacaaatc cgcaacatcg
      121 ccatcatcgc ccacgtcgac cacggcaaaa ccacattggt cgaccaactg ctgcgccaat
      181 ccggcacatt ccgcgccaac cagcaggttg acgagcgcgt gatggacagc aacgacctcg
      241 aaaaagaacg cggcatcacc atcctcgcca aaaacaccgc catcgattac gaaggctacc
      301 acatcaatat cgtcgacacg ccgggacacg ccgacttcgg cggcgaagta gagcgcgttt
      361 tggggatggt ggactgcgtc gtcttgttgg tggacgcgca ggaaggcccg atgccgcaaa
      421 cccgtttcgt gaccaaaaaa gccttggctt tggggctgaa accgattgtc gtcatcaaca
      481 aaatcgacaa gccgtccgct cgtccgagct gggtcatcga ccaaactttc gagctgttcg
      541 acaacttggg cgcgaccgac gagcagttgg atttcccgat tgtttatgct tccggtctgt
      601 ccggtttcgc caaattggaa gaaaccgacg agagcaacga catgcgtccg ctgttcgata
      661 ctatcttaaa atatacgcct gcaccgagcg gcagcgcgga cgaaacgctg caactgcaaa
      721 tttcccaact cgactacgac aactacaccg gccgcctcgg tatcggtcgt atcttgaacg
      781 gacgaatcaa acccggccaa accgttgccg tcatgaacca cgatcagcaa atcgcccaag
      841 gccgcatcaa ccagcttttg ggtttcaaag gtttggaacg cgtgccgctt gaagaagccg
      901 aagccggcga catcgtgatt atttccggta tcgaagacat cggtatcggc gtaaccatca
      961 ccgacaaaga caatcccaaa ggcctaccga tgttgagcgt ggacgaaccg acgctgacga
     1021 tggactttat ggtcaacacc agcccgctgg cgggtacgga aggcaaattc gtaaccagcc
     1081 gccaaatccg cgaccgcctg caaaaagaat tgctgaccaa cgtcgccctg cgcgtggaag
     1141 ataccgccga tgccgacgtg ttccgcgtat ccgggcgcgg cgagctgcac ctgaccattt
     1201 tgctggaaaa catgcgccgc gaaggctacg aactcgccgt cggcaaaccg cgcgtcgtgt
     1261 accgcgacat cgacggtcaa aaatgcgaac cgtatgaaaa cctgaccgtg gatgtacccg
     1321 acgacaacca aggcgcggta atggaagaac tcggccgccg ccgtggcgaa ctgactaata
     1381 tggaaagcga cggcaacgga cgcacccgcc tcgaatacca tattccagcg cgcggcttga
     1441 tcggtttcca aggcgaattt atgaccctga cgcgcggggt cgggctgatg agccatgtgt
     1501 tcgacgatta cgcgcccgtc aaacccgata tgcccggccg ccacaacggc gtgctggtgt
     1561 cccaagagca gggcgaggca gtcgcttacg ccttgtggaa tctggaagac cgcggtcgta
     1621 tgttcgtatc acccaacgac aaaatctacg aaggtatgat tatcggcatc cacagccgcg
     1681 acaacgattt ggtggtcaac ccgctcaaag gcaaaaaact taccaacatc cgcgccagcg
     1741 gcaccgacga agccgtgcgc ctgaccacgc cgatcaagct gacgctggaa ggtgcggttg
     1801 agtttatcga cgatgacgaa ctcgttgaaa tcacgccgca atccatccgt ctgcgcaagc
     1861 gttacttgag cgaattggaa cgccgccgcc actttaaaaa gctggattga tgtttactgg
     1921 attaatgttt aaatgatacg cgatgccgtc tgaaaaattt cagacggcat tttttattcg
     1981 gacgggcttt gcggcttctt gaagctgttt cagacggcgt ttttcctacc caatcaagaa
     2041 actgccgcca tttttccagc ggtatatcgc cccgtcgcgt ttcggtatcg ggttcggctt
     2101 cccggcagca tgtcgaacaa tgccgtttga aggaatacgc cttttgagtc cttacaagct
     2161 taggaagagg atatagtgga ttaacaaaaa tcaggacaag gcgacgaagc cgcaga
//
LOCUS       DAXHSF010000217         2135 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.217, whole genome shotgun sequence.
ACCESSION   DAXHSF010000217 DAXHSF010000000
VERSION     DAXHSF010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.217"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            373..885
                     /locus_tag="ACFM8S_001968"
     CDS             373..885
                     /locus_tag="ACFM8S_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFC6314979.1"
                     /translation="MKNFNGKLILIGLMGAGKTTLGRQMAQRLDYRFYDSDHEIAVAA
                     GVPIPTIFEMEGEQGFRSRETAILKKLVILPHIVLSTGGGAVLKEENRALIRKSGTVV
                     YLHAPPETLLERTRCDNSRPLLQVADPLAKLRELYAARDPVYRQTADFTVESANCRQT
                     VQTLLKRLSR"
     gene            965..2044
                     /gene="aroB"
                     /locus_tag="ACFM8S_001969"
     CDS             965..2044
                     /gene="aroB"
                     /locus_tag="ACFM8S_001969"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFC6314980.1"
                     /translation="MKTLTVHTPSHSYPIFIGNGLLPQAGSLLKPHLGKRAAIIANET
                     VAPLYLGTLQTALDAAGVSHFSIILPDGEAHKNWQTLNLIFDGLMQNRAERKTTLIAL
                     GGGVIGDMVGFAAATYQRGAPFVQIPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     QAVLADLDTLHTLPVRELSAGMAEVIKYGALGDIGFFEWLEQNISGLMTLDREKLAQA
                     VYRCCQMKADIVAQDETEQGIRAWLNLGHTFGHAIETEMGYGTWLHGEAIAAGCVLAA
                     RLSEQLGKTSAADTARLAALLEAAGLPSAPPVFAFEKWLEHMSHDKKVSGGIMRFIGL
                     NRLGEANITEITDTDILRRTLQPYL"
BASE COUNT          525 a          670 c          517 g          423 t
ORIGIN      
        1 ttcagcacct tagagaatcg ttctctttga gctaaggcga ggcaacgctg tactggtttt
       61 tgttaatcca ctatagtatt gataaacata ttatcttcaa tatattcaat tggataattg
      121 tttacctaag caaagataat tgccttttcc tgacaaataa gtgaaatcaa cggattgtca
      181 aaacacagcc tgaaataaaa aacctccctg atttctttta tttttcctta aaatcagaaa
      241 ggttcgggat ggtcgggtta tttttccaaa cgtaccgctg ccctgccgat ttcgtataaa
      301 attccgccgt aacccgacaa gcccgaaccc tgtcgccccg aaaggcgggg tgtcaaacat
      361 taaggaattg tgatgaaaaa ctttaacggc aaactcatcc tcatcggact gatgggcgcg
      421 ggcaaaacca cgctgggccg gcaaatggcg cagcggctgg attaccgttt ttacgacagc
      481 gatcacgaaa tcgccgtagc cgccggcgtt cccatcccca ccatatttga aatggaaggc
      541 gaacagggat tccgttcgcg cgaaaccgcc atactcaaaa agctggttat cctgccccat
      601 atcgtcctgt ccaccggcgg cggcgcggtg ttaaaagaag aaaaccgcgc ccttatccgc
      661 aaaagcggca cggtcgtcta tctgcacgcc ccgcccgaaa ccctgctcga acgcacgcgc
      721 tgcgacaaca gccgtccttt gctgcaagtt gccgatcctt tggcgaaatt acgtgaactc
      781 tacgccgcac gcgaccccgt ttaccgccaa accgccgact ttaccgtaga atcggcaaac
      841 tgccggcaaa ccgtgcaaac cctgctcaaa cgcttatccc gataaaccgg catatgcgcc
      901 gcgcccagaa aaccaagccg cgccccgccc ggcgggccgg cggttcaaac tttaaggaac
      961 aacaatgaaa acactgaccg tacacacccc gtcccacagc taccccatct ttatcggcaa
     1021 cggactgctg ccgcaggcag gaagcctgct caaaccgcat ttgggcaaac gcgccgccat
     1081 catcgccaac gaaaccgtcg ccccgctcta cctcggcacg cttcagacgg cattggatgc
     1141 ggcaggcgta tcccatttca gcatcatcct gcccgacggc gaggcgcaca aaaactggca
     1201 gacgctcaac ctcatctttg acgggctgat gcaaaaccgc gccgaacgca aaaccacatt
     1261 aatcgcactg ggcggcggcg tgatcggcga catggtcggc tttgccgctg ccacctacca
     1321 gcgcggcgca ccgttcgttc aaataccgac cacactgttg agtcaagtcg actcatcggt
     1381 gggcggcaaa accgccatca accacccgct cggcaaaaat atgatcggcg cattctacca
     1441 gccgcaggcg gtgcttgccg acttggacac gctgcacacc ctgcccgtcc gcgaattgtc
     1501 cgccggtatg gcggaagtca tcaaatacgg cgcgctcggc gacatcggtt ttttcgaatg
     1561 gctcgaacaa aacatatccg gactgatgac gctcgatcgg gaaaaactcg cccaagccgt
     1621 gtaccgctgc tgccaaatga aggcagacat cgtcgcccaa gacgaaaccg aacagggcat
     1681 acgcgcatgg ctcaacctcg gacacacctt cggacacgcc attgaaaccg agatgggtta
     1741 cggcacttgg ctgcatggag aagccatcgc cgccggctgc gtgttggcgg cgcgtttgtc
     1801 cgaacaactg ggcaaaacct ccgccgcaga taccgcgcgg ctcgccgccc tgctcgaagc
     1861 cgccggactg ccgtccgcac cgcccgtgtt tgcctttgaa aaatggctcg aacatatgag
     1921 ccacgataaa aaagtcagcg gcggcatcat gcgctttatc ggtctgaacc ggctgggcga
     1981 agccaacatc accgaaatta ccgacacgga catcctccgc cgcaccctgc aaccgtatct
     2041 ctgatttcct ctgccgatgt gctgccgcgc gggtttgacg cacgatgatg attttccatc
     2101 atctttctcc gtaaaagcgg gaatccggtc cgttc
//
LOCUS       DAXHSF010000218         2065 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.218, whole genome shotgun sequence.
ACCESSION   DAXHSF010000218 DAXHSF010000000
VERSION     DAXHSF010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.218"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(<1..161)
                     /locus_tag="ACFM8S_001970"
     CDS             complement(<1..161)
                     /locus_tag="ACFM8S_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC6314981.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTAAELVGINKNTAAYYFHR
                     LRLLIYQNSP"
     gene            422..1894
                     /gene="pyk"
                     /locus_tag="ACFM8S_001971"
     CDS             422..1894
                     /gene="pyk"
                     /locus_tag="ACFM8S_001971"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFC6314982.1"
                     /translation="MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVR
                     FNFSHGTPEFHQENARIVREAAKRAGQEIAIIADLQGPKIRVGKIAGGGIELNKGETL
                     LLDAALEGEGTREAVGLDYRDLPDDVAAGDVLWLDDGLLTLTVESVEGSRIITRVENS
                     HVLKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKV
                     EEEMKGSTAVRPGLVSKIERVEAIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQK
                     RMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGA
                     YPFETVSQMAIICAAAEKEQDSLNGVAEQAEYPEAVSTNLAVAGGAVSVARAVHAKAI
                     VALTESGSTAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNE
                     VETMLVEHNILHSGDQYIITSGSQMRESGSTNTLEVLHVK"
BASE COUNT          540 a          577 c          536 g          412 t
ORIGIN      
        1 ggactgtttt gataaataag taatcgtaaa cgatgaaaat aataggctgc ggtattttta
       61 ttaatgccta ctaactctgc tgctgttctt gcagttacac ctgcgacaaa tagctcaatg
      121 agtttatttt gtttataccg gcttagacga ctttttctca tagggataat tctaacttaa
      181 tttgaatttc cctagttatc taggacagcc ccaaaaataa atttagccta agtcataaaa
      241 ccgtgtaatt ttgcaccaaa aaaggtggtt tgcaggtaaa atatcaaacc attaatttaa
      301 atcatatttt tacagaatat tccgccgcgt tcatcaaatg gacatcaaac cggttccaaa
      361 tttctgtaat tttgtaacaa aataccgcaa aacacccgat tgagaccaaa aggactttca
      421 tatgaaccag acaagccgcg atctgacccg catcagccac aacaccaaaa tcgtcgccac
      481 ccttgggccg ggcagcaaca acgtcgaact gttggaagat atgatccgcg tcggcggcct
      541 gaacgtcgtc cgcttcaact tcagccacgg cacgcccgaa ttccatcagg aaaacgcccg
      601 catcgtgcgc gaagcggcaa aacgcgccgg acaggaaatc gccatcattg ccgacctgca
      661 agggccgaaa atccgcgtgg gcaaaatcgc cggcggcggc atcgaattga acaaaggcga
      721 aacgctgcta ctggatgccg cgcttgaagg cgaaggcaca cgcgaggcgg tcggtttgga
      781 ctaccgcgac ctgcccgatg acgttgccgc aggcgatgtc ttgtggctgg acgacggcct
      841 gctgaccctg accgtggaat ccgtcgaagg cagcaggatt atcacaaggg tggaaaacag
      901 ccacgtcctg aaaagcaaca agggcatcaa caaacgcggc ggcggtctgt ccgcaggcgc
      961 gttgaccgaa aaagacttcc gcgacctgaa aaccgcgatt gccatcggtt gcgactatct
     1021 cgccatcagc tttgtgaaat ccgccgaaga tttgcacatc gcgcgcgcca aagtcgaaga
     1081 agaaatgaag ggcagcactg ccgtgcgccc tggtttggtt tccaaaatcg aacgcgtaga
     1141 agcgattgaa aaccttgatg aaatcatcct cgccggcgac ggcattatgg ttgcgcgcgg
     1201 cgacttggcg gtcgaagtcg gacacgccgc cgtccccgcc ctgcaaaaac ggatgatccg
     1261 ccgcgcccgc gagttgcgcc gcttcagcat tacggcgacg caaatgatgg aatcgatgat
     1321 taccaacccc gtgccgaccc gcgcggaagt cagcgatgtg gcaaacgcgg tattggacgg
     1381 caccgatgcg gtgatgtgtt ccgccgaaac cgccgtcggc gcgtatccgt ttgaaaccgt
     1441 cagccaaatg gcaattatct gcgcggctgc ggaaaaagag caggattcgc tcaacggcgt
     1501 tgccgaacag gcggagtatc ccgaagcggt cagcaccaac ctggcggttg ccggcggcgc
     1561 ggtcagcgtg gcgcgcgcgg ttcacgccaa agccatcgtc gccctgaccg aaagcggctc
     1621 gaccgccttt gaaatcagcc gccacaacat caccctgccg attttcgcgc tgaccccgag
     1681 cgtttccgcc caacgccgta tggcgatgta ccggggcgtg cgcccgctga ttttggcaac
     1741 cagtaccgac cacgacacgg cgttgaacga agtcgaaacg atgctggtgg aacacaacat
     1801 cctgcattcc ggcgaccagt acatcatcac cagcggttcg caaatgcgcg aatccggttc
     1861 gaccaacaca ctggaagtgc tgcacgtcaa ataatccgcc ctgagtggaa aatgccgtct
     1921 gaagccgatg cccgaggctt cagacggcat tttttgcggc ggcgcggcgg ttcaggcaaa
     1981 ctgaaaaaat tgtgcaaatc cggcaacatc agataaaatt gagtacctat actaaagcga
     2041 aacaaggcat ttccgactgc ctttt
//
LOCUS       DAXHSF010000219         1905 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.219, whole genome shotgun sequence.
ACCESSION   DAXHSF010000219 DAXHSF010000000
VERSION     DAXHSF010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.219"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(217..1035)
                     /gene="cysE"
                     /locus_tag="ACFM8S_001972"
     CDS             complement(217..1035)
                     /gene="cysE"
                     /locus_tag="ACFM8S_001972"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFC6314983.1"
                     /translation="MKKDHLNTTGFDLWHTIREETAAAVSAEPMLASFLHQTVLRHES
                     LGSVLAYHLSSKLGSPIMDVRALFEIYQQALGSDTQIGKCVEADLKAIYERDPACDEY
                     SLPLLYFKGFHAIQAHRINHRLYLDGRKTLAYFLQNRMSEVFGVDIHPAARFGYGLML
                     DHATGFVAGETAVLGNNISILHGVTLGGSGKEGGDRHPKIGDGVMIGANASILGNIRI
                     GSNAKIGAGSVVVSDVPPSITVVGVPAKPVARSLKTPSADMDQNIQFAEIDFMI"
     gene            1219..1797
                     /gene="grpE"
                     /locus_tag="ACFM8S_001973"
     CDS             1219..1797
                     /gene="grpE"
                     /locus_tag="ACFM8S_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFC6314984.1"
                     /translation="MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPAYED
                     LQARIAELEAQLKDEQLRALANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEM
                     ALLDQSGNFDALKMGVQMTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQ
                     EPNTVVGVMKKGYTLSDRVLRPAMVTVAQKEA"
BASE COUNT          436 a          436 c          581 g          452 t
ORIGIN      
        1 ttccgtacta tccgtactgt ctgcggcttc gtcgccttgt cctgattttt gttaatccac
       61 tatatttccg gttgcgtggg aatcgggtgt atcgaataaa aggcattttg tccgactggc
      121 aagtgccggt atcggcggca tatcaaggcg caggcttgaa gcgggcaatg ccgtctgaag
      181 cacgtttggc gtttcagacg gcattggtgc ggatattcaa atcataaagt cgatttcggc
      241 aaactggata ttttgatcca tatccgccga cggtgttttg agcgatcgcg ccacgggttt
      301 ggcgggtacg ccgacaaccg tgatggacgg cggcacgtct gaaaccacga cgctgcccgc
      361 cccgattttg gcattgctgc cgatgcggat attgcccaat atcgaggcgt ttgcgccgat
      421 catcacgccg tcgccgattt tagggtggcg gtcgccgcct tctttgcccg aaccgccgag
      481 cgttacgccg tgcaaaatcg aaatattgtt gcccaacacg gcggtttcgc cggcaacaaa
      541 gccggtggcg tggtcgagca tcagcccgta tccgaaacgg gcggcgggat ggatgtccac
      601 gccgaatact tcggacatac ggttttgcag gaaatacgcc agcgttttgc gcccgtcgag
      661 atacagccga tggttgatgc ggtgtgcctg aatcgcgtgg aagcctttga aatataaaag
      721 cggcagcgaa tattcgtcgc aggcgggatc gcgttcgtag atggctttta agtctgcttc
      781 gacgcatttg ccgatttggg tgtcgctgcc caacgcctgc tggtagattt caaacagcgc
      841 gcgcacgtcc ataatcgggc tgccgagttt gctggaaagg tggtaggcaa ggacggagcc
      901 gagggactcg tggcgcaaca cggtttggtg caaaaaactt gccagcatcg gttcggcgga
      961 gaccgcggcc gcggtttctt cgcggatggt gtgccagagg tcgaaaccgg ttgtgtttaa
     1021 atggtctttt ttcatgagtg atgacgtttg aaaatcgata tggtcggcag tatcttaccg
     1081 tctatattat tttttcggta ggggatttga aaatgaattt gaaattctct gcttttgctt
     1141 gaagtttctt gaaaatgtcc ttatcttgcg cgggtaataa ctggattttg atttccaatt
     1201 tgttttaagg gatacgatat gagcgaacag acacagcagc aaaacagtga agaagcggtt
     1261 gaaaatgtgg aggcggtgga aaccgtcgag acagtaggaa atgcggacgg tgtgcaggaa
     1321 caggctgccg cagagccggc ttatgaggat ttgcaggcgc ggattgccga gctggaagcg
     1381 cagttgaaag acgagcagct gcgcgctttg gcaaacgagc aaaacctgcg ccgccgccac
     1441 cagcaggaaa ttgcggatac gcacaagttc gccggacaga agtttgccgt ggaaatgctg
     1501 ccggtcaagg attatctgga aatggcgctt ttggatcaga gcggcaattt cgatgcgctg
     1561 aaaatgggcg tgcagatgac tttgaacgag ttgcagaaag catttgatgc tacgcaaatc
     1621 aaggaaatca accctaaagc gggcgataag ctcgatccga atatccatca ggcgatgcag
     1681 gcggtggcaa gcgaacagga gccgaatacc gtggtgggtg tgatgaagaa gggttatacg
     1741 ctgtccgacc gcgtgttgcg cccggctatg gttacggtgg cgcagaagga agcctgaagg
     1801 cgtctgggga ataatctgat ttatttcctg aagcgcgttt tgcgtataaa ccgatcgaag
     1861 taaagcggca atgccgtctg aacccgcctg tcgggcttca gacgg
//
LOCUS       DAXHSF010000220         1864 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.220, whole genome shotgun sequence.
ACCESSION   DAXHSF010000220 DAXHSF010000000
VERSION     DAXHSF010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.220"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            220..885
                     /locus_tag="ACFM8S_001974"
     CDS             220..885
                     /locus_tag="ACFM8S_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFC6314985.1"
                     /translation="MSIKYIAMPAAWKYMPTDVSRVAKEVFGYLYNSQGASTRFDKYF
                     NTIDNRADFFAASNLYEQNLGSKARWFGGADFVSRAAITGLGADGEASYITFAAGKVV
                     GNPPIYEWRAASGNALIVNGFYNFRDLFNKKTNPREWDIQQLKSEQKLLQPIHQKYLS
                     NEKDYLSLIKGVTSNKIFSIIPNPLDERKKIEDGINMLDYKSRIKYGCKLMGYSEKQG
                     CKP"
     gene            882..1316
                     /locus_tag="ACFM8S_001975"
     CDS             882..1316
                     /locus_tag="ACFM8S_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314986.1"
                     /translation="MNKNYLFYLIQNIKSIFLIGGVFFGLLIFSGLWGNDFKLSFLGV
                     IDFIFGMVGGGFIVMLWFLIPVILSTLFRWVFQNQLAVSVILTFIVFYTYGYFSLFNS
                     SERVGSFWYLVIPTIMFIGLSEWFIYKSSVHSLGENVTPRSN"
     gene            complement(1384..1652)
                     /locus_tag="ACFM8S_001976"
                     /pseudo
     CDS             complement(1384..1652)
                     /locus_tag="ACFM8S_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244123.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            1651..1824
                     /locus_tag="ACFM8S_001977"
     CDS             1651..1824
                     /locus_tag="ACFM8S_001977"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314987.1"
                     /translation="MIKQVANSLPDGSPAKAAVFKAEREGKLKTAIAGVDRQTGKAVI
                     LSVKVPSKTNIRR"
BASE COUNT          570 a          272 c          409 g          613 t
ORIGIN      
        1 gcggtttgcc ggaagaaggt gctcatgaga aatcccctaa atgtcttggt gggaatttag
       61 gggattttgg ggaattttgc aaaggtctct tgttctacta cgatggaaaa agtaaatgcc
      121 atcaaaaagg atgaacaatt tagcaaagta attgatacgg aaattcaaaa agtctgttct
      181 aggaacccat tgggcgatgg ttgcagaaac ggcattaata tgtctattaa atatattgcc
      241 atgcctgctg cgtggaagta tatgcctacg gatgtatcac gggttgccaa agaagttttt
      301 ggctatttat ataactcaca aggggcatct acaagatttg acaagtattt caacaccatt
      361 gacaatcgtg cagatttctt tgctgccagc aatctttatg agcaaaattt gggttcaaaa
      421 gcacgatggt ttggtggagc tgattttgta tcgcgtgctg ctataactgg gttaggggca
      481 gacggagaag cttcttatat aacttttgcg gcaggtaaag ttgttggtaa tcctccaatt
      541 tatgaatgga gggctgcgtc aggcaatgca ctgatagtaa atggatttta taattttaga
      601 gacttgttca ataaaaaaac taatcctagg gagtgggata ttcaacagtt gaaaagcgag
      661 caaaagttat tacagcctat tcaccaaaaa tatttgagta atgagaagga ttatttgtca
      721 ttgattaaag gggtaacatc aaataagata ttttcaataa ttccaaatcc tttagatgag
      781 aggaaaaaaa tagaggatgg aattaatatg ttagattaca aatctagaat taaatatggt
      841 tgtaagctta tgggatattc tgagaaacag ggatgcaagc catgaataaa aattatttat
      901 tttatcttat tcaaaatatt aaatctattt tcttaatagg tggagtattt tttggccttt
      961 taattttttc aggtttatgg ggtaatgatt ttaaactatc ttttttaggg gtaatagatt
     1021 ttatatttgg gatggtaggg ggtgggttta tagttatgtt atggtttcta attccagtga
     1081 tattatcaac tttattcaga tgggtatttc aaaatcaact tgctgtatct gtcatattaa
     1141 ccttcattgt tttttataca tatgggtact tttctttatt taattcatct gaaagagtcg
     1201 gtagtttttg gtatttggta atcccaacca taatgtttat tggactgtct gaatggttta
     1261 tctataaatc tagcgtgcat agtttaggtg aaaatgtaac tccacgttcc aattgaagaa
     1321 aaagattgtc tgaaaactaa atttaatttc agatgacctt agattcggat ttcaagtaca
     1381 acactagggt atcgatggtt ggaacagatt caagaataaa acacttggcg tttcgtagcc
     1441 aagtgttttt ctcggtcggt ggttcaactc atcttgaacc ctacgtatct cccgatcgct
     1501 gatgtttcgg aaaacggttt gtttggggaa tattgtcgga tgagtccatt ggtgttttca
     1561 ttcagccctt tctcccaaga atggtaaggg cggtaaaaat aggtttccgc cttcaatgtt
     1621 ttggccattt ggatgtgtcg gtagaatgct ttgattaaac aagttgcaaa tagcttacct
     1681 gatggtagtc ctgctaaagc tgctgtcttc aaagcggagc gggaaggcaa attaaaaacg
     1741 gcaatagcag gcgttgatcg tcaaacaggt aaggccgtta ttctttctgt caaagttcct
     1801 tctaaaacca atataaggag ataacaatgg ggcacaatat gatgaccacc caaaaatggt
     1861 atga
//
LOCUS       DAXHSF010000221         1779 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.221, whole genome shotgun sequence.
ACCESSION   DAXHSF010000221 DAXHSF010000000
VERSION     DAXHSF010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.221"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(546..1604)
                     /gene="alr"
                     /locus_tag="ACFM8S_001978"
     CDS             complement(546..1604)
                     /gene="alr"
                     /locus_tag="ACFM8S_001978"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFC6314988.1"
                     /translation="MRPLNVQIRLGNLRHNYRILKEMHGGKLLAVVKADAYGHGAVRC
                     AFALADLADGFAVATIDEGIRLRESGITHPIVLLEGVFEASEYEAVEQYSLWSAVGNQ
                     WQLEALLARHWKKPVKVWLKMDSGMHRTGFFPHDYASAYAALKQSEYVDSIVKFSHFS
                     CADEPESGMTEIQMEAFDLGTEGLEGEESLANSAAILNVPEARRDWGRAGLALYGISP
                     FGGSDDRLKPVMRLSTRIFGERVLQPHSPIGYGATFYTSKSTRVGLIACGYADGYPRR
                     APSNSPVAVDGKLTRVIGRVSMDMMTIELDASQEGLGHEVELWGDTVNINTVAEAAGT
                     IPYELMCNIKRAKFTYIE"
BASE COUNT          447 a          450 c          404 g          478 t
ORIGIN      
        1 aaaaaatccc tgtgtcctta tttctgcttt cttacgtcat tcttttaaag gtgggaatct
       61 ggaaaccaaa aaacaacagg aatttatccg aaacaacaaa acctatccgc cggcatcccc
      121 gcgaaagcgg gaatctagtt tgttgttcaa ataaattctg aaactttaat tttgtcattc
      181 ccgcgcaggc gggaatctag aaatgaaaaa cgacgagaat ttatcagaaa tgacggaaac
      241 tcaaaaaacc ggattcccgc ctgcgcggga atgacgaaat tttaggtttc tgattttgat
      301 tttccgtttt tgcgggaagg atggggtttc agattgtggg cattatcagt attttctatt
      361 tggcaatgtc ttttataatg gattaaattt aaaccagtac ggcgttgcct cgccttgccg
      421 tacgatttgt actgtctgcg gcttcgtcgc cttgtcctga tttttgttaa tccactatac
      481 tgattttgtc aaaacgcatt tatgccgtct gaaaggcttc agacggcatt tttgtttgga
      541 cttgattact cgatataagt gaattttgca cgtttgatat tgcacatcaa ttcgtaaggg
      601 atggttccgg ccgcttcggc aacggtattg atattaaccg tatcgcccca cagttcgacc
      661 tcgtgtccca aaccttcttg cgaagcgtcc agctcgatgg tcatcatatc catagagacc
      721 ctgccgatga cccgggtcaa tttaccgtcg acggcgacgg gggaattgct tggggcgcgg
      781 cgcggataac cgtccgcata accgcaggca atcaggccga cgcgcgtaga tttactggta
      841 taaaatgttg cgccataacc gataggggag tgcggctgta aaacgcgttc gccgaaaata
      901 cgggtggaaa gcctcatcac gggtttcagc ctgtcatcgc tgcctccgaa cggggaaatg
      961 ccgtacaacg ccaagccggc tcgcccccag tccctgcgtg cttcgggaac attcaaaata
     1021 gcggcggagt tggcaaggct ttcttcgcct tccagccctt ccgtacccaa atcgaatgct
     1081 tccatctgta tttccgtcat accgctttcg ggttcgtccg cacaggagaa atgcgagaat
     1141 ttgacaatac tgtccacata ttccgactgc ttcaatgccg catatgccga agcgtaatca
     1201 tgagggaaaa atccggtacg gtgcattccc gaatccattt tcaaccagac cttgacaggt
     1261 tttttccaat ggcgggccag caaagcctca agctgccatt ggtttccgac tgccgaccag
     1321 agcgagtatt gttcgaccgc ttcgtactcc gatgcttcaa atacgccttc caaaaggaca
     1381 atcggatggg taatgccgct ctcacgcagc ctgattccct catcgattgt cgccacggca
     1441 aagccgtctg ccaagtctgc cagcgcgaaa gcacatctga ccgcaccgtg tccgtatgca
     1501 tccgccttaa ctaccgccaa cagcttgcct ccgtgcattt cctttaaaat ccgataattg
     1561 tgcctaaggt tgcccaacct gatctgcacg ttcaaagggc gcataaattc attccttacc
     1621 aaacaagtgt ttattagcaa aattaagaaa tcggttgggg aattataatc caacggcaag
     1681 gcttttaaaa tattaaagac ccttgtgtct tcaagccgga gcagcagttt tcaggtagga
     1741 aggcggtttg cttttcctga tataatcgct ttcccaaat
//
LOCUS       DAXHSF010000222         1767 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.222, whole genome shotgun sequence.
ACCESSION   DAXHSF010000222 DAXHSF010000000
VERSION     DAXHSF010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.222"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(286..1398)
                     /gene="porB"
                     /locus_tag="ACFM8S_001979"
     CDS             complement(286..1398)
                     /gene="porB"
                     /locus_tag="ACFM8S_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric porin PorB"
                     /protein_id="HFC6314989.1"
                     /translation="MKKSLIALTLAALPVAAMADVTLYGTVKAGVEVSRVKNQGVKNQ
                     GVKNQGVKNQGVKTKTATQIADFGSKIGFKGQEDLGNGMKAIWQLEQKASIAGTNSGW
                     GNRQSFIGLKGGFGTVRAGNLNTVLKDSGDNVNAWESGSNTENILGLGTIGRVESREI
                     SVRYDSPVFAGFSGSVQYVPRDNANDKDKYTHMKSSRESYHAGLKYENAGFFGQYAGS
                     FAKYADLNTDAERVAVNTANAHPVKDYQVHRVVAGYDANDLYVSVAGQYEAAKNNEVG
                     STAIGKKHEQTQVAATAAYRFGNVTPRVSYAHGFKAKVNGVKKASYQYDQVIVGADYD
                     FSKRTSALVSAGWLKEGKGVNKTEKTASMVGLSHKF"
BASE COUNT          422 a          391 c          429 g          525 t
ORIGIN      
        1 ccgtgcggat ggattcgtca ttcccgcgca ggcgggaatc taggtctgtc ggtgcggaaa
       61 cttatcgggt aaaacggttt cttgagattt tgcgttctag attcccactt tcgtgggaat
      121 gacgggatgt aggttctcgg gaatgacggc ggagaagttt ctgctttccc gataaatgcc
      181 cactctcgtg ggaatgaagg ctgccgcatt aaatagcaaa cgaatcattt caaataaaaa
      241 agcctgtctg gcgacaggct ttttgttgat accaatcttt tcagattaga atttgtgact
      301 caagccaacc atgctggcag ttttttcagt tttgtttacg cctttgcctt ctttcaacca
      361 accggcagaa accagagcgg aagtgcgttt ggagaagtcg tagtcggcac caacgataac
      421 ttggtcgtat tggtaacttg cttttttcac gccattcact ttagctttga agccgtgagc
      481 gtaagaaacg cgaggcgtta cgttgccaaa acggtaagcg gcagtagcgg caacttgagt
      541 ttgctcgtgt tttttaccaa tagcggtaga accaacctcg ttgtttttag cagcttcata
      601 ctgaccggca acagaaacgt acaggtcatt agcatcgtaa ccggcaacta cgcggtgtac
      661 ttggtaatcc ttaacaggat gggcatttgc agtatttact gcaacacgtt ctgcatcagt
      721 gttcaaatca gcatatttgg caaaagagcc tgcgtattga ccgaagaaac cagcattttc
      781 gtatttcaga ccagcgtggt aagactcacg gctggacttc atatgtgtgt atttatcctt
      841 atcattcgca ttgtcgcgcg gaacgtattg tacgctgccg ctgaagcctg caaatacggg
      901 agagtcgtag cgtacggaga tttcacggct ttctacacga ccgatagtac ccagtcccag
      961 tatattttcg gtgttagaac cagattccca tgcattgacg ttgtcgccgc tgtctttcaa
     1021 tacggtgttc agattaccgg cgcggacggt accgaagccg cctttcaagc cgatgaagga
     1081 ctggcggtta ccccagccgc tgttagtgcc ggcgatggag gctttttgtt ccaactgcca
     1141 aatggctttc atgccgttgc cgaggtcttc ttgacctttg aaaccgattt tagaaccgaa
     1201 gtcggcaatt tgggttgcag ttttggtttt tacgccttga ttttttacgc cttgattttt
     1261 tacgccttga ttttttacgc cttgattttt tacgcgagaa acttctacac ccgctttaac
     1321 tgtaccgtac agggtaacat cagccattgc tgcaacaggc agggcagcca gtgtcagggc
     1381 aatcagggat tttttcattg ctgtattcct tttcatagtt aagaaattta agcagaccaa
     1441 actttccaat ccgcttaggc tttacttcta ccgccgatgt cggcgattcc atgtctgaaa
     1501 ctataaatgt tcctgtctga aaaaacaaac ttctgcctgt ttttgtgtgg aatccgatgt
     1561 gcattttgaa gggcggatag agatattgtg ggtatttttt gttttataac atatggttat
     1621 ttaaattttt taagatttgc atttttacaa cacttactcg ggagggtatt ggagggcatt
     1681 gcaaaccggg ggttataaag acggcaaaaa acgcgcacgt ggtttctttc ggaacgggcg
     1741 gatgcaaaac ccgcccctgc ggcagga
//
LOCUS       DAXHSF010000223         1762 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.223, whole genome shotgun sequence.
ACCESSION   DAXHSF010000223 DAXHSF010000000
VERSION     DAXHSF010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.223"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..537
                     /locus_tag="ACFM8S_001980"
     CDS             <1..537
                     /locus_tag="ACFM8S_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HFC6314990.1"
                     /translation="TQAAEAGAGIATGAVTVGNAWEAPVGALSKAKAAKQAIPTQTVK
                     ELDGLLQESKNIGAVNTRINIANSTTRYTPMRQTGQPVSAGFEHVLEGHFHRPIANNR
                     SVFTISPNELKVILQSNKVVSSPVSMTPDGQYMRTVDVGKVIGTTSIKEGGQPTTTIK
                     VFTDKSGNLITTYPVKGN"
     gene            544..1053
                     /locus_tag="ACFM8S_001981"
     CDS             544..1053
                     /locus_tag="ACFM8S_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6314991.1"
                     /translation="MSNFEKKYILELNDALSHLNHNSTSFDLLKVLISWLSNDIVIDK
                     FKILGYDFSKYIEMNPDDYPVEKSILNREEIIYLKNNIYRKISSGNFKFQYFVQYIRD
                     ILEYLFIEHIERVCPYCEWGEMQKLEEQNTHETVYLCTQCGCAFYNDNSQFLLKTPLT
                     IPMKRDEFK"
     gene            1122..>1762
                     /locus_tag="ACFM8S_001982"
     CDS             1122..>1762
                     /locus_tag="ACFM8S_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HFC6314992.1"
                     /translation="MIYGSMPSEKLTIFQTAFVMQVNIQIPCMLYRRGSVKPPLFEAP
                     RLLPSFTDPVVPKLSAPGGYIVDIPKGNLKTEIEKLSKQPEYAYLKQLQVAKNVNWNQ
                     VQLAYDKWDYKQEGLTGAGAAIVVIIVTALTYGYGAAAAGSVTAAGSSTAAAATTTAA
                     ATTTVSTAAAMQTAALASLYSQAAVAIINNKGDVGKALKDLGTSDTVKQIVTSA"
BASE COUNT          565 a          364 c          367 g          466 t
ORIGIN      
        1 actcaggctg ccgaagcggg cgcgggaatc gccacgggtg cggtaacggt aggcaacgct
       61 tgggaagcgc ctgtgggggc gttgtcgaaa gcgaaggcgg ccaagcaggc tataccaacc
      121 cagacagtta aagaacttga tggcttacta caagaatcaa aaaatatagg tgctgtaaat
      181 acacgaatta atatagcgaa tagtactact cgatatacac caatgagaca aacgggacaa
      241 ccggtatctg ctggctttga gcatgttctt gaggggcact tccataggcc tattgcgaat
      301 aaccgttcag tttttaccat ctccccaaat gaattgaagg ttatacttca aagtaataaa
      361 gtagtttctt ctcccgtatc gatgactcct gatggccaat atatgcggac tgtcgatgta
      421 ggaaaagtta ttggtactac ttctattaaa gaaggtggac aacccacaac tacaattaaa
      481 gtatttacag ataagtcagg aaatttgatt actacatacc cagtaaaagg aaactaacta
      541 aatatgagta actttgaaaa aaaatatatt ttagaattaa atgatgcttt aagccattta
      601 aatcataact ctacctcatt tgatttattg aaagttttga tttcatggtt atcaaacgat
      661 attgtcattg ataaatttaa aattttaggt tatgacttta gtaaatatat cgaaatgaat
      721 cccgatgact atccggttga aaaatctata ttgaatagag aggaaattat ttatctcaaa
      781 aacaatattt atcgtaaaat atcatcagga aattttaaat ttcaatactt tgtacaatat
      841 attagagata ttttagaata tttatttatt gaacatattg aaagagtctg tccttactgc
      901 gaatggggtg aaatgcaaaa attagaagaa caaaatacgc atgaaacggt gtatctctgt
      961 actcaatgtg gatgtgcttt ttataacgat aattcacaat ttttattaaa aaccccttta
     1021 accattccaa tgaaacgtga tgaatttaaa taaacaagcc gtagcctgca tgaaccctaa
     1081 aatccacgtg tagcgtgtgt gcgccagcac gcatgcgttc catgatttac ggctcaatgc
     1141 cgtctgaaaa gctcacaatt tttcagacgg catttgttat gcaagtaaat attcagattc
     1201 cctgtatgct gtacagacgc gggagtgtta agcccccctt gtttgaagct ccgcggctcc
     1261 tgccgagctt caccgacccc gttgtgccca agctctctgc tcccggcggc tacattgtcg
     1321 acatccccaa aggcaatctg aaaaccgaaa tcgaaaagct gtccaaacag cccgagtatg
     1381 cctatctgaa acagctccaa gtagcgaaaa acgtcaactg gaaccaggta cagctggcgt
     1441 acgacaaatg ggactataaa caggaaggct tgaccggagc cggtgcagcg attgtggtga
     1501 ttattgtaac cgctctaact tatggatacg gagcggctgc agcgggtagc gtaactgccg
     1561 caggaagtag tacagccgca gctgcaacaa cgacagcggc agcaactacc actgtttcta
     1621 ctgcagctgc catgcaaacc gcagctttag cctccttgta tagccaagca gctgtagcca
     1681 tcatcaataa taaaggcgat gtaggcaaag cattaaaaga tctcggcacc agtgatacgg
     1741 tcaagcagat tgttacttcc gc
//
LOCUS       DAXHSF010000224         1755 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.224, whole genome shotgun sequence.
ACCESSION   DAXHSF010000224 DAXHSF010000000
VERSION     DAXHSF010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.224"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(114..770)
                     /locus_tag="ACFM8S_001983"
     CDS             complement(114..770)
                     /locus_tag="ACFM8S_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HFC6314993.1"
                     /translation="MKNKTKVWDLPTRLFHWLLAASLPFMWYSAKAGGDMLQWHTRVG
                     LFILFLLVFRLCWGIWGSDTARFSRFVRGWSGIREYMKNGIPEHVQPGHNPLGALMVV
                     ALLAAVSFQIGTGLFAADENTFSTNGYLNHLVSEHTGSLMRKIHLNFFKLLAVFSAIH
                     IAAVAAYRVFKKKNLVLPMITGFKYIEGKTSIRFAGKTALAAALSVAALVAAAILLLS
                     "
     gene            834..1724
                     /locus_tag="ACFM8S_001984"
     CDS             834..1724
                     /locus_tag="ACFM8S_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFC6314994.1"
                     /translation="MFPVFHLSGESRRRMLQTALRFPYVFKARAEDSHKGTFGTLAVV
                     GGSAGMSGAPVLAASAAMYLGCGKVWAGFNQDALPFAVIAGFPEIMLDTADGLTKRQG
                     INAWTAGCGLGTDKAAVGTLAGILTEHTDKPVLLDADALNILSTDAETRNLARGCKNL
                     ILTPHHAEAARLLGTTVAQVQADRTAAVRKIRAIFGATVVLKGHKTLVASPDTEIYVN
                     ESGNAGLATAGSGDVLGGIIGSLLAQGVPVFEAACAGAWLHGAAADVIKESAGIAAGL
                     LAGEIAPAARWLRNRITESM"
BASE COUNT          393 a          418 c          581 g          363 t
ORIGIN      
        1 ggtttgggac ggaaaaacgg cctgaagccg tttcgggcat tcagaccgtt tgcgcggaga
       61 ggggatgccg tccgaagggc gaaaagggct tcagacggca ttgatgtcgg gtttcaggac
      121 aggagcagga tggcggctgc gacaagcgcg gcaaccgata atgcggcggc aagcgcggtt
      181 ttgccggcaa agcggattga ggttttgcct tcgatgtatt tgaagccggt tatcatcggg
      241 aggacgaggt ttttcttttt gaacacgcgg tatgcggcga cggcggcgat atgaatggcg
      301 gaaaaaacgg cgagcagctt gaaaaagttg aggtggattt tccgcataag gctgcccgta
      361 tgctcggaaa ccaaatggtt gaggtagccg ttggtgctga aggtgttttc atcggcggca
      421 aaaagccctg tgccgatttg aaatgagacg gcggccaaaa gcgcgacgac catcagtgcg
      481 cccaaggggt tgtgtccggg ttggacgtgt tcgggaatac cgtttttcat atactctctg
      541 atacccgacc atccgcggac gaaacgggag aaacgggcgg tatcgctgcc ccaaatgccc
      601 cagcagaggc ggaatacgag caggaaaagg ataaacagcc cgacgcgcgt gtgccattgc
      661 agcatatcgc cgccggcttt tgcgctatac cacataaagg gcaaggacgc ggcaagcagc
      721 cagtggaaaa ggcgggtggg gaggtcccag actttggttt tgtttttcat aatcggtttc
      781 cggcggtaga atcggtttgt tttcgagcct tattttaacc gattggaggg gcaatgtttc
      841 ccgtttttca tctttcaggc gagagccgcc gccggatgct tcagacggca ttgcgttttc
      901 cctatgtttt caaagcccgt gcggaagatt cgcacaaagg gactttcggc acgctcgccg
      961 tagtcggcgg atcggcaggg atgagcggtg cgcccgtatt ggcggcatcg gcggcgatgt
     1021 atctcggctg cggcaaggtg tgggcgggtt tcaatcagga tgcgcttccg tttgccgtca
     1081 ttgccggttt ccccgaaatt atgttggaca cggcagacgg tttgaccaag cgtcaaggca
     1141 taaacgcttg gacggcgggt tgcggcttgg gtacggataa ggcggcggtc ggaacgcttg
     1201 ccggaatttt gacggaacac acggacaagc ccgtcctttt ggatgcggac gcgctgaaca
     1261 tattatcaac cgatgccgaa acccgaaatc tggcacgcgg gtgtaaaaac ctgattttaa
     1321 cgccacacca cgccgaagcc gcgcgcctgc ttggaacgac ggttgcacag gttcaggcgg
     1381 atcggacggc ggcagtgagg aagataaggg caattttcgg cgcaaccgtg gttttaaagg
     1441 ggcacaaaac attggttgcc tcacccgata cggaaatcta tgtcaacgaa agcggcaacg
     1501 cgggattggc aacggcgggc agtggcgacg tattgggcgg catcatcggc agtctgctcg
     1561 cacagggcgt gccggttttt gaagccgcct gcgcgggcgc gtggctgcac ggcgcggcgg
     1621 cggatgtcat aaaagaatcg gcaggcattg cggcagggct gttggctggc gaaatcgccc
     1681 cggcggcaag gtggctgcgc aaccggataa ctgaaagtat gtaagaagat atagtggatt
     1741 aacaaaaacc agtac
//
LOCUS       DAXHSF010000225         1747 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.225, whole genome shotgun sequence.
ACCESSION   DAXHSF010000225 DAXHSF010000000
VERSION     DAXHSF010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.225"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(19..942)
                     /gene="lpxC"
                     /locus_tag="ACFM8S_001985"
     CDS             complement(19..942)
                     /gene="lpxC"
                     /locus_tag="ACFM8S_001985"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFC6314995.1"
                     /translation="MLQRTLAKSISVTGVGLHSGERVALTLHPAPENSGISFRRTDLD
                     GEMGEQIKLTPYLINDTRLSSTIVTDKGVRVGTIEHIMSALSAYGIDNALIELNAPEI
                     PIMDGSSLPFIYLLQDAGVVDQKAQKRFLKILKPVEIKEAGKWVRFTPYDGFKVTLTI
                     EFDHPVFNRSSPTFEIDFAGKSYIDEIARARTFGFMHEVEMMRAHNLGLGGNLNNAIV
                     IDDTDVLNPEGLRYPDEFVRHKILDAIGDLYIVGHPIVGAFEGYKSGHAINNALLRAV
                     LADETAYDRVEFADSDDLPDAFHELNIRTCG"
     gene            complement(1556..>1747)
                     /locus_tag="ACFM8S_001986"
     CDS             complement(1556..>1747)
                     /locus_tag="ACFM8S_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFC6314996.1"
                     /translation="SSNVNNEIKGKKLSLWAKRQDGSVKWFCGQPVKRDATATDDDVT
                     ANINTKHLPSTCRDDSSVT"
BASE COUNT          432 a          432 c          454 g          429 t
ORIGIN      
        1 cggaaaaaac agaaagcgtt atccacaagt tctgatgttc agctcgtgaa atgcgtcggg
       61 caaatcatcg ctgtcggcaa attccacccg gtcgtaagcc gtttcgtctg ccaaaactgc
      121 gcgcaagagt gcgttgttga tggcgtgtcc cgatttgtag ccttcaaacg cgccgacaat
      181 cggatgtccg acgatataca aatcaccgat ggcatcaagg attttgtggc gcacaaactc
      241 atcgggatag cgcaagcctt caggattcag gacatccgtg tcgtcaatca cgatggcgtt
      301 gttcaaattg ccgcccaaac ccagattgtg ggcgcgcatc atttccactt cgtgcataaa
      361 gccgaaggtg cgcgcgcgcg cgatttcgtc gatgtaggat ttgccggcga aatcaatttc
      421 aaaagtgggc gagctgcggt tgaaaaccgg atggtcgaat tcgatggtca gcgttacctt
      481 aaagccgtca tacggcgtaa agcgcaccca tttgcccgct tctttgattt cgacaggctt
      541 gaggattttc aaaaaacgct tttgcgcctt ttgatcgacc acgcccgcat cttgcaaaag
      601 gtaaataaac ggcaggctgg agccgtccat aatcgggatt tcgggcgcgt tcagctcaat
      661 cagtgcgttg tcgataccgt aggcggacag cgcggacata atgtgttcga tcgtgccgac
      721 gcgcacgcct ttgtcggtaa cgatggtgga tgaaaggcgg gtatcgttga tcaaataagg
      781 ggtcagcttg atttgttcgc ccatctcgcc gtccaaatcg gtgcgtcgga aggaaatccc
      841 gctgttttca ggcgcggggt gcagggtcag cgcgacgcgt tcgcccgaat gcagcccgac
      901 tccggtaacg ctgatggatt tcgccaaagt tctttgcagc ataaaccgct cccttatcaa
      961 tggggtaagt tttggaataa tacgataaaa ccggaaaaac aagctatgtt tttccatagt
     1021 atttgccaat gtatccgttt tcaatacgta agccgcataa aaatgtatag tggattaaca
     1081 aaaatcaaga caaggcgacg aagccgcccc tcccgaaaaa cgcaaaaaat gccgtccgaa
     1141 aacctttcgg acggcatttt cgcgtgaacc gtcattccca caaggacaaa aaaccaaaaa
     1201 cagaaaccta aaattcgtca ttcccgcaaa agcgggaatc cagttcgttc ggtttcgctt
     1261 gttttaagtt tcgggtaact tccacttcgt cattcccgcg caggcgggaa tccagtccgc
     1321 ttgttcggta aatgagaggg cggattgcgc gcctgtcaga taaaccccgt gtttaaacgg
     1381 gcggcaatga ggtacgcgca gagccttgaa gcgcaatcga tatattattt tcagccaaaa
     1441 cggacgcccc cgcttgcctt gcaaaccctt aaaatgaagc cacccggatt aatccgagtg
     1501 gccgtggaaa atcacttacc gcttgattta tttaaaattt atggtataat ttgccttagg
     1561 tgacagatga atcatcgcgg caggttgacg gcaggtgctt ggtgttgatg ttggcggtga
     1621 cgtcgtcgtc ggtggcggtg gcgtcgcgct taaccggctg tccgcagaac cattttaccg
     1681 aaccgtcttg acgcttggcc cacagggaga gttttttgcc tttgatttcg ttgtttacgt
     1741 tgcttga
//
LOCUS       DAXHSF010000226         1732 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.226, whole genome shotgun sequence.
ACCESSION   DAXHSF010000226 DAXHSF010000000
VERSION     DAXHSF010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.226"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            356..1660
                     /locus_tag="ACFM8S_001987"
     CDS             356..1660
                     /locus_tag="ACFM8S_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFC6314997.1"
                     /translation="MKKHIWAASLLPASLSAEPLNWWKPYSAVNSGDTAWVMTAAALV
                     LLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGL
                     GRVFLSGMQIDAAAQMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
                     MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRR
                     IGYGREAMPPHNMAMTLIGAAMLWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAA
                     GWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAIGILTAAACFVSVTV
                     VKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGFVMA
                     AYSGLMSWAILKVVGKICGGLRVGKDVEREGLDLNIHGERVE"
BASE COUNT          354 a          434 c          511 g          433 t
ORIGIN      
        1 aattaaaaat ttttaatata ttaatcaata aattaatttt ataaaataaa aatattgtca
       61 acaatatttt gccttatcgc ccaaacctct gtatattttc ctacagtaaa ttgttgacaa
      121 tccatacgcc cacatatgcg ccgcctaagg ataaatcctc ccgccggaca acgggtgcaa
      181 gggattggat gcgatatttc catattcaaa caagggattt gtttcacgca cagggcggca
      241 catcggcaaa atccccgcgc cgccccggtc cggcagggct tgcgtccctc ccggacaagc
      301 cccgaccccg cctttccgaa agacgggctc aaccattaag gaaactttaa tcaaaatgaa
      361 aaaacacata tgggcggcat ctttgctgcc ggcatcccta tcggcagaac ctttaaactg
      421 gtggaagcct tattccgccg tcaattcggg cgataccgcc tgggtgatga ctgcggctgc
      481 cttggtactg ttgatgacgc ttcccgggct ggctttattc tacggcggta tggtgcggaa
      541 aaaaaacctg ctctcgacga tgatgcacag cttttccatc gcgacattgg tgggaatcct
      601 ttgggtcgcc gtcggctatt ctttagcgtt cacgccggga aatgccttta tcggcggttt
      661 ggggcgcgta tttttaagcg ggatgcagat agacgctgcc gcacagatgc tgaccgtgtc
      721 gcccaatgcg ccgactgttc ccgaaccggt atttatgttt tttcagatga cgtttgccat
      781 tatttcgacc gccattatta ccggcgcgtt tgccgaacgg atgaaatatt cggcaatgat
      841 gctgttttcg ggcatatggt ttttattggt ttatgtgccg ggcgcgcatt gggtctgggg
      901 cggcggcttt atgagcaagg gcggcgtatt ggattatgcc ggcggtacgg tggtgcacat
      961 caatgccggt atcgcgggac tcgtcgccgc cttggttttg ggcaggcgca taggctacgg
     1021 gcgcgaggcg atgcctccgc acaatatggc gatgacactg atcggcgcgg caatgttgtg
     1081 gttcggctgg ttcggcttta acgccggatc ggcgcttgcg gcagacgcgg cggcgggtat
     1141 ggcgatggcg gtaacgcagg tgtcggccgt attcggcgcg gcaggctggc ttgcctgcga
     1201 aaaaatagcg ggacacaaac cttccgcttt ggggctggct tccggcgcgg tttccggtct
     1261 ggtcggcatc acccctgccg ccggctttac cggcccgtcg ggcgcggccg ccatcggtat
     1321 attgactgcc gccgcgtgct ttgtgtccgt caccgtcgtc aaacacaaat tgcgttacga
     1381 tgattctttg gacgctttcg gcatacacgg attcggcggg ctggtgggcg gaatattgac
     1441 cggcatcttt ttcgacaacc gcattttcgg cggggatgcg gcagtttggc agcagttgtg
     1501 gatacaggta aaagacgggt tcgttatggc ggcatacagc gggctgatga gttgggcgat
     1561 tttgaaggtc gtggggaaaa tctgcggcgg cctgcgcgtc ggcaaggatg tcgaacgcga
     1621 aggtttggat ctgaatatcc acggcgaacg cgtggaataa gggcggctat gccgtctgaa
     1681 gcctgaaaat cgggtttcag acggcatttt tcacgtttgc cgccgatgga ta
//
LOCUS       DAXHSF010000227         1722 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.227, whole genome shotgun sequence.
ACCESSION   DAXHSF010000227 DAXHSF010000000
VERSION     DAXHSF010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.227"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..465
                     /locus_tag="ACFM8S_001988"
     CDS             <1..465
                     /locus_tag="ACFM8S_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX iron-regulated FrpC family protein"
                     /protein_id="HFC6314998.1"
                     /translation="LEPYNQQNKAHFIQFLRDGLDSVDDIVIRKDACSLSTTMGERLL
                     TYGVKKMPSAYPEYEAYEDKRHIPENPYFHEFYYIKKGENPAIITHWNNRVNQAEEDN
                     YSTSVGSCINGFTVQYYPFIREKQQLTQQELVGYHQQVEQLVQSFVNNSSKK"
     gene            482..>1722
                     /locus_tag="ACFM8S_001989"
     CDS             482..>1722
                     /locus_tag="ACFM8S_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated protein frpC"
                     /protein_id="HFC6314999.1"
                     /translation="MNEGEVVLTPEQIQTLRGYASRGDTYGGWRYLANLGDRYADDAA
                     AIVGKDANLNGLNLWMKKGVENLWDDTVGKKTRLEKFDRVALQHFRQYARLINENNGR
                     LPNTSEIERSYYKAVTDNGVSSSAAIDLVINRSLPDMADGYWALGLGIEAERIHNEQA
                     VNNPNGSEWDNRKQLISALDKGFDGSFKEKPFTFLQSVIMDVTKLSVEYTIDGWQKIG
                     GWGNGIINDLYKSVAKREWTGIFEIVNNNIKQFRDLFPNPEGWIDDGHQCFAPWVKET
                     KKRNGKYHVYDPLALDLDGDGIETVATKGFAGSLFDHTNNGIRTATGWVSADDGLLVR
                     DLNGNGIIDNGAELFGDNTKLADGSFAKHGYAALAELDSNGDNIINAADAAFQTLRVW
                     QDLNQDGISQANELRTLEELG"
BASE COUNT          541 a          309 c          406 g          466 t
ORIGIN      
        1 ctcgaaccat acaaccagca gaacaaagcg cactttatcc aatttctacg cgatggtttg
       61 gatagcgtgg acgatattgt tatccgaaaa gatgcgtgta gtttaagcac gactatggga
      121 gaaagattgc ttacttacgg ggttaaaaaa atgccatctg cctatcctga atacgaggct
      181 tatgaagata aaagacatat tcctgaaaat ccatattttc atgaatttta ctatattaaa
      241 aaaggagaaa atccggcgat tattactcat tggaataatc gagtaaacca ggctgaagaa
      301 gataattata gcactagcgt aggttcctgt attaacggtt tcacggtaca gtattacccg
      361 tttattcggg aaaagcagca gctcacacag caggagttgg taggttatca ccaacaagta
      421 gagcaattgg tacagagttt tgtaaacaat tcaagtaaaa aataatttaa aggatcttat
      481 tatgaatgag ggtgaagttg ttttaacacc agaacaaatc caaaccttgc gtggttatgc
      541 ttcccgtggc gatacctatg gcggttggcg ttatttggct aatttgggtg accgttatgc
      601 ggatgatgct gctgcaattg tcggtaagga tgcaaactta aatggtttga atttatggat
      661 gaaaaaaggt gtggaaaacc tatgggatga tacggtcggt aaaaagaccc gtttagagaa
      721 atttgatcgg gttgcactgc aacatttcag gcaatatgcg cgtctaatta atgaaaataa
      781 tggtagatta cccaatacta gtgaaattga gagaagttac tataaagccg ttaccgataa
      841 tggcgtttct tccagtgcag ctattgattt agttattaat cgttcacttc cggatatggc
      901 ggatggttat tgggcattag gtttggggat agaagccgaa cgtatccaca atgagcaagc
      961 agtaaataat ccgaacggta gcgaatggga taatagaaag cagttaatat ctgctttaga
     1021 taaaggattt gatggatctt ttaaagagaa gccttttact tttttacaat ctgtgataat
     1081 ggatgtaaca aagttaagtg ttgaatatac aatagatggt tggcaaaaaa ttggaggttg
     1141 gggtaatggg ataatcaatg atttatataa aagtgttgca aaaagagagt ggactggaat
     1201 atttgagatc gttaataata acatcaagca atttagagat ctgttcccaa atccggaagg
     1261 ctggatcgat gatggtcacc aatgtttcgc tccttgggtt aaagaaacta aaaaacgcaa
     1321 tggcaaatat catgtctacg accccctcgc cctagatttg gacggagacg gtatagaaac
     1381 cgttgccacc aaaggctttg caggcagctt atttgatcac accaacaacg gcatccgcac
     1441 cgccaccggt tgggtttctg ccgatgatgg tttgcttgtg cgcgatttga acggcaacgg
     1501 catcatcgac aacggtgcgg aactcttcgg cgacaatacc aaactggcag acggttcttt
     1561 tgccaaacac ggctacgcgg ctttggccga attggattca aacggcgaca acatcatcaa
     1621 cgcggcagac gccgcattcc aaaccctgcg tgtatggcag gatctcaatc aggacggcat
     1681 ttcccaagct aatgaattgc gtacccttga agaattgggt at
//
LOCUS       DAXHSF010000228         1713 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.228, whole genome shotgun sequence.
ACCESSION   DAXHSF010000228 DAXHSF010000000
VERSION     DAXHSF010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.228"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            64..1437
                     /locus_tag="ACFM8S_001990"
     CDS             64..1437
                     /locus_tag="ACFM8S_001990"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFC6315000.1"
                     /translation="MSNPFSSLGLGTELVSALTAQGYENPTPIQAAAIPKALAGHDLL
                     AAAQTGTGKTAAFMLPSLERLKRYATASTSPAMHPVRMLVLTPTRELADQIDQNVQGY
                     IKNLPLRHTVLFGGVNMDKQTADLRAGCEIVVATVGRLLDHVKQKNISLNKVEIVVLD
                     EADRMLDMGFIDDIRKIMQMLPRQRQTLLFSATFSAPIRKLAQDFMNAPETVEVAAQN
                     TTNANVEQHIIAVDTFQKRNLLERLIVDLHMNQVIVFCKTKQSVDRVTRELVRRNLSA
                     QAIHGDRSQQSRLETLNAFKDGSLRVLVATDIAARGLDIAELPFVINYEMPAQPEDYV
                     HRIGRTGRAGADGVAISLMDESEQKMFESIKELTGNKLLIERIEGFEPQWWEQGGAKP
                     EKTEMREPRQRNRYESARAQREKNTRPETTANDAGAACGKIAGRSRRSRREHRTCALL
                     QPRYGVK"
     gene            1617..>1713
                     /locus_tag="ACFM8S_001991"
     CDS             1617..>1713
                     /locus_tag="ACFM8S_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009118462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HFC6315001.1"
                     /translation="MSTQFDVAVIGAGPAGSVASALLRKKGYSGLN"
BASE COUNT          435 a          561 c          412 g          305 t
ORIGIN      
        1 tttccccatc ctatcggttt cccgcttcag acggcataag gtctgaaaga aagactacaa
       61 tatatgagta atccattttc ctctttaggt ttgggtacgg aactcgtttc cgcactgacc
      121 gcgcaaggtt acgaaaaccc gacgcccatc caagccgccg ccattcccaa agcactcgcc
      181 ggtcatgatt tactagccgc cgcgcaaacc ggcacgggca aaaccgccgc ctttatgctg
      241 ccctcgctcg aacgcctcaa gcgttacgcc accgccagca cctcgcccgc gatgcacccc
      301 gtgcgtatgc tcgtgttgac cccgacgcgc gaacttgccg accaaatcga ccaaaacgtg
      361 cagggctaca ttaaaaacct gccgctgcgg cataccgtcc tcttcggcgg tgtcaacatg
      421 gacaaacaaa ccgccgacct gcgtgccggc tgcgaaatcg tcgtcgccac cgtcggacgg
      481 ctgctcgacc atgtgaaaca gaaaaacatc agtttaaaca aagtcgaaat cgtcgttttg
      541 gacgaagccg accgtatgct ggatatgggt tttatcgacg acatccgcaa aatcatgcag
      601 atgctgcccc gccaacgcca aaccctgctc ttttccgcca ccttctccgc cccgatacgc
      661 aaactggcgc aagacttcat gaacgcgccc gaaaccgtcg aagtcgccgc gcaaaacacc
      721 accaacgcca acgtcgaaca acacatcatc gccgtcgata ccttccaaaa gcgcaacctg
      781 ctcgaacggc tgattgtcga tttgcatatg aaccaggtca tcgtgttctg caaaaccaaa
      841 caaagcgtcg accgcgtaac gcgcgaactg gtgcgccgca acctgtccgc acaggcgata
      901 cacggcgacc gttcccaaca aagccggctc gaaacactca acgccttcaa agacggcagc
      961 ctgcgcgtcc tcgtcgccac cgacatcgcc gcgcgcgggc tggacattgc cgaactgccc
     1021 ttcgtcatca attacgaaat gcccgcccag cccgaagact acgtccaccg catcgggcgc
     1081 acggggcgcg cgggcgcgga cggcgtggcg atttccctga tggacgaatc cgaacagaaa
     1141 atgtttgaat ccattaaaga gctgaccggc aacaagctgc tcatcgagcg catcgagggc
     1201 ttcgagccgc aatggtggga acagggcggc gcaaaaccgg aaaaaaccga aatgcgcgaa
     1261 ccgagacaac gcaaccgcta cgaatccgcc agggctcagc gcgaaaaaaa tacccgaccg
     1321 gaaaccacgg caaacgatgc gggcgcggct tgcggaaaaa ttgccggacg cagccgccga
     1381 agccgtcggg aacaccggac gtgcgccctg ctccaaccgc gttacggcgt aaaataaccc
     1441 tgaaaatcaa atgccgtctg aacatttccc gtttcagacg gcatttttca aaccggactg
     1501 acgcattggg agcaaccgcc cgcaccggat aaatttcttc cgcaaacagt ttcagacggc
     1561 atttgctgcc tgtacaatat catttccttt tccactcaaa ccatcaggat ttcccaatgt
     1621 ccacccaatt cgatgtcgcc gtaatcggcg caggtccggc aggctcggtc gcatccgccc
     1681 tgctccgcaa aaaaggttat agtggattaa att
//
LOCUS       DAXHSF010000229         1696 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.229, whole genome shotgun sequence.
ACCESSION   DAXHSF010000229 DAXHSF010000000
VERSION     DAXHSF010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.229"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(27..350)
                     /locus_tag="ACFM8S_001992"
     CDS             complement(27..350)
                     /locus_tag="ACFM8S_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFC6315002.1"
                     /translation="MDMVVRGGIYLVSLDPTVGSEIKKTRPCVVVSPPEIHNYLKTVL
                     IVPMTSGSRPAPFRVNVRFQDKDGLLLPEQIRAVDKAGLVKHLGNLDNSTAEKLFAVL
                     QEMFA"
     gene            complement(369..578)
                     /locus_tag="ACFM8S_001993"
     CDS             complement(369..578)
                     /locus_tag="ACFM8S_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="growth regulator"
                     /protein_id="HFC6315003.1"
                     /translation="MILNIRKMGNSQGVILPKSLLGQIGAVDSLAVTVEKGNIILSCP
                     TVRRGWAEAAAMLVETEQEHFFFRN"
     gene            complement(701..1498)
                     /gene="truA"
                     /locus_tag="ACFM8S_001994"
     CDS             complement(701..1498)
                     /gene="truA"
                     /locus_tag="ACFM8S_001994"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFC6315004.1"
                     /translation="MDTAQKQRWAITLSYDGSRFYGWQKQAGGVPTVQTALETALAQI
                     AGEFVATTVAGRTDTGVHATAQVVHFDTTAVRPQQAWVRGVNAHLPEGIAVLHARQVA
                     PEFHARFDAYGRHYRYLLESAPVRSPLLKNRAGWTHLKLDIEPMRRAAAYLIGEQDFS
                     SFRAAECQAKSPVKTIYRADLTQSAGLVRLDLHGNAFLHHMVRNIMGALVYVGSGRLS
                     VEGFAALIQERSRLKAPPTFMPDGLYLTGVDYPEAYGIIRPKIPEWL"
BASE COUNT          373 a          404 c          492 g          427 t
ORIGIN      
        1 gaacacaatc cattcagact attcaatcag gcaaacatct cctgcaatac tgcaaacagt
       61 ttttcagccg tactgttgtc taaattgcca agatgcttga ccaatccggc tttatccaca
      121 gccctaatct gttcgggcaa aagcaaaccg tctttatcct gaaagcggac attgacgcgg
      181 aacggggcag gacggcttcc gctcgtcatg ggaacgatca gcacagtctt gagatagttg
      241 tgtatttcag gaggagagac tacgacacaa ggacgtgtct ttttgatttc gcttcctacg
      301 gtcgggtcta aggagaccag atagattccg ccgcgtacta ccatatccat tctttatccg
      361 cttcgttttc aatttcggaa aaaaaaatgc tcctgctcgg tttcgacaag cattgcggca
      421 gcttctgccc atcccctgcg aacggtagga cagcttaaaa taatattgcc cttttcaact
      481 gtaacagcca agctgtctac tgcccctatt tgacccaata atgatttggg cagaatcacg
      541 ccttgcgagt ttcccatttt gcgtatgttg agaatcatat acgtacccta tttaccagtt
      601 tatgtaataa caatgttatt accttgatga ggtagtgtca acattgaaaa agattgcctg
      661 acagtttgtc cgatttcaaa atctccgcga caagaatgtt ttaaagccat tcggggattt
      721 tggggcggat gatgccgtat gcctcgggat agtcgacgcc ggtcaggtaa agcccgtcgg
      781 gcatgaaggt tggtggggct ttgaggcggc tgcgttcttg aatcagtgcg gcgaagcctt
      841 cgacgctgag cctgccgctg ccgacataaa cgagcgcgcc catgatgttg cgtaccatat
      901 ggtgcaaaaa ggcgttgccg tgcaaatcga ggcggacgag tcctgcgctt tgggtaaggt
      961 cggcgcggta gatggttttg acgggggatt ttgcctggca ctcggcggcg cggaagctgg
     1021 agaagtcttg ttcgccgatc aaataggcgg cagcccgacg catcggttcg atgtcgagtt
     1081 tgaggtgtgt ccagcctgcc ctgtttttga gcagggggga acggacgggg gcggattcga
     1141 gcaggtagcg gtagtgtcgt ccgtatgcgt caaatcgtgc atgaaattcg ggggcgacct
     1201 gtcgagcatg caaaacggca attccttcgg gcaggtgggc atttacgccg cgcacccatg
     1261 cctgttgggg acggacggca gttgtgtcaa agtggacgac ttgggcggtg gcgtgcacgc
     1321 cggtatcggt tctgccggca acggtggtgg cgacaaattc tcctgcgatt tgggcgagtg
     1381 cggtttccaa tgccgtctga acggtcggta cgccgcccgc ctgtttctgc cagccgtaaa
     1441 agcggctgcc gtcataggat agggttattg cccagcgttg tttttgtgcg gtatccatcg
     1501 gatttgggat tcggataaat gttcagactg cattgtatcg cagattttgc agggaaacgg
     1561 caaacgccca gggcgagcgg cgttgtttgg ggagttgggg ggggatgcag ttgctacgaa
     1621 tcgctatcct gtgaattcac cctgtcagga gtgcccgaat cgtcattccc gcgcagtcgg
     1681 gaatctagga cgtaaa
//
LOCUS       DAXHSF010000230         1624 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.230, whole genome shotgun sequence.
ACCESSION   DAXHSF010000230 DAXHSF010000000
VERSION     DAXHSF010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.230"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(20..682)
                     /locus_tag="ACFM8S_001995"
     CDS             complement(20..682)
                     /locus_tag="ACFM8S_001995"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFC6315005.1"
                     /translation="MIHAVLFDLDGTLADTALDLGGALNTLLARHGLPAKSMDEIRTQ
                     ASHGAAGLIKLGAGITPDHPDYARWRTEYLEEYDRRYAQDTTLFGGVNELIAELGKRG
                     IKWGIITNKPMRFTDKLVPKLGFIVPPAVVVSGDTCGEPKPSVKPMLYACGQIHADPQ
                     HTLYVGDAERDIQAGRNAGMKTVLAEWGYIAPEDDTGSWQADFHIRTPLDLLECLDKI
                     QS"
     gene            complement(695..1522)
                     /locus_tag="ACFM8S_001996"
     CDS             complement(695..1522)
                     /locus_tag="ACFM8S_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFC6315006.1"
                     /translation="MKPDIYALLERALLSGDPDEKGRLTDEAFAAVQNADGAEANAPP
                     ADFPRAGRPDKPVLVAPSQLTPRKMNTAEGYAAMLHAIAHIEFNAVNLALDAAYRFRT
                     LPFQFVRDWVKVAKEEVYHFRLMRDRLRTFGFDYGSFEAHNHLWDMAYKTAYDPLLRM
                     ALVPRVLEARGLDVTPGIRAKVAQRGDSATCGVLDIIYRDEVGHVAIGNHWYQHLCRE
                     RGLEPITLFRSLIARYDMFIFRGYVNIEAREKAGFSRFELDMLEDFEQGLKQNKNTV"
BASE COUNT          305 a          436 c          504 g          379 t
ORIGIN      
        1 tttgtggggc ggatattttt caggactgta ttttgtccag acattcgagc agatcgagcg
       61 gcgtgcggat gtggaaatcc gcctgccatg agccggtatc gtcttcggga gcgatgtagc
      121 cccattcggc gaggacggtt ttcatgccgg cgttgcggcc tgcttggatg tcgcgttcgg
      181 catcaccgac gtagagtgtg tgttgcgggt cggcgtggat ttgtccgcac gcatacagca
      241 tgggtttgac gctgggcttg ggttcgccgc aggtgtcgcc gctgacgacg acggcgggtg
      301 ggacgatgaa gccgagtttg gggacgagtt tgtcggtgaa gcgcatgggt ttgttggtga
      361 tgatgcccca tttgatgccg cgttttccga gttcggcgat gagttcgttt acgccgccga
      421 agagggtggt gtcttgggcg tagcggcggt cgtattcctc cagatattcg gtgcgccatc
      481 gggcatagtc ggggtggtcg ggggtgatgc ctgcgccgag tttgatcagt cctgccgcgc
      541 cgtggctggc ttgggtgcgg atctcgtcca tgctttttgc aggtagtccg tggcgagcga
      601 gcagggtgtt gagtgcgcct ccgaggtcga gggcggtgtc ggcaagtgtg ccgtcgaggt
      661 cgaagagtac ggcgtgtatc atgtgtgttc cttttcagac ggtatttttg ttctgtttca
      721 aaccctgctc gaaatcttcc agcatatcca attcaaagcg gctgaagccc gctttttcgc
      781 gcgcttcgat gttcacatag ccgcggaaga taaacatatc gtaacgggca atcagactgc
      841 ggaacagggt aatcggttcc aaaccgcgtt cgcggcagag gtgttgatac caatggttgc
      901 cgatggcgac atgacccact tcgtcgcggt aaatgatgtc caacacgccg caggttgccg
      961 aatcgccgcg ctgcgccacc ttcgcgcgta tccccggcgt aacgtccagc ccgcgcgctt
     1021 ccaacacgcg cggcaccaaa gccatacgca acagtgggtc gtaggcggtt ttgtacgcca
     1081 tatcccacaa atggttgtgc gcctcgaaac tgccgtaatc gaagccgaaa gtgcgcaatc
     1141 tgtcgcgcat caggcggaaa tggtacacct cttccttcgc cactttcacc cagtcgcgga
     1201 caaactgaaa cggcagcgtg cggaaacggt atgccgcgtc caaagccaga ttgacggcgt
     1261 tgaattcgat atgcgcaatc gcgtgcagca tcgccgcata gccttctgcc gtgttcattt
     1321 tgcgcggggt cagttgcgac ggcgcgacca aaacaggctt gtccggccgt cccgcgcggg
     1381 ggaagtccgc cggtggcgcg tttgcttccg ccccgtccgc attttgaacg gcggcaaacg
     1441 cctcatccgt cagccgtcct ttttcatctg ggtcgcccga aagcagggcg cgttccagca
     1501 aagcataaat atcgggtttc atttcaagtc cgccgtgttc ggaaaacaaa tattatagcg
     1561 tttaaaaaaa acaagatgag gcatataatc tccgcgattc ggcattccgc gcccaaaccg
     1621 tcaa
//
LOCUS       DAXHSF010000231         1510 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.231, whole genome shotgun sequence.
ACCESSION   DAXHSF010000231 DAXHSF010000000
VERSION     DAXHSF010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.231"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            34..1437
                     /locus_tag="ACFM8S_001997"
     CDS             34..1437
                     /locus_tag="ACFM8S_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HFC6315007.1"
                     /translation="MTPSALKKTVLLLGTAFAAASAQASGYHFGTQSVNAQSTANAAA
                     AEAADASTIFYNPAGLTKLDSSQISVNANIVFPSIRYEADSATDFTGIPVQGSKSGKI
                     TKTTVAPHIYGAYKVNDNLTVGLGVYVPFGSATEYEKDSVLRHNINKLGLTSIAVEPV
                     AAWKLNDRHSFGAGIIAQHTSAELRKYADWGIKSKAEILTAKPPKPNGVAEAAKIQAD
                     GHADVKGSDWGFGYQLAWMWDINDRARVGVNYRSKVSHTLKGDAEWAADGAMAKQLWD
                     ANKLALLGYTPSEKARVKIVTPESLSVHGMYKVSDKADLFGDVTWTRHSRFDKAELVF
                     EKEKTVVKGKSDRTTITPNWRNTYKVGFGGSYQISEPLQLRAGIAFDKSPVRNADYRM
                     NSLPDGNRIWFSAGMKYHIGKNHVVDAAYTHIHINDTSYRTAKASGNDVDSKGASSAR
                     FKNHADIIGLQYTYKFK"
BASE COUNT          403 a          491 c          351 g          265 t
ORIGIN      
        1 ttatccaccg tccgcctttt tacggaaacc gaaatgaccc cttccgcact gaaaaaaacc
       61 gtcctgctgc tcggcactgc ctttgccgcc gcatccgcac aagcctccgg ctaccacttc
      121 ggcacacagt cggtcaacgc gcaaagcacg gcgaatgccg ccgccgcaga agccgccgac
      181 gcatcgacca tcttctacaa ccccgccggc ctgaccaaac tcgacagcag ccagatttcc
      241 gtcaacgcca atatcgtatt tccaagcatc cgttatgaag cggattccgc caccgacttt
      301 accggcattc ccgtccaagg ttcgaaaagc ggcaaaatca ccaaaaccac ggttgcgccc
      361 cacatctacg gcgcatacaa agtcaacgac aatctgaccg taggcttggg cgtgtacgtc
      421 cccttcggct ctgccaccga atacgaaaaa gattccgtat tgcgccacaa catcaacaaa
      481 ctcggtctga ccagcatcgc cgtcgaacct gtcgccgcgt ggaaactcaa cgaccgccat
      541 tccttcggcg caggcatcat cgcccaacat acttccgccg aactgcgcaa atatgccgac
      601 tgggggatta agagtaaagc agagatattg acggcaaaac cgcccaaacc taacggtgta
      661 gccgaagctg caaaaattca ggccgacgga cacgccgatg tcaaaggcag cgattggggc
      721 ttcggctacc aactggcgtg gatgtgggac atcaacgacc gtgcgcgcgt gggcgtgaac
      781 taccgttcca aagtctcgca cacgctcaaa ggcgatgccg aatgggcggc agacggcgca
      841 atggcgaaac agttatggga tgcaaacaaa ctcgcactgc tcggctacac gccaagcgaa
      901 aaagcccgcg ttaaaatcgt tacgcccgag tctttgtccg tacacggcat gtacaaagtg
      961 tccgacaaag ccgacctgtt cggcgacgta acttggacgc gccacagccg cttcgataag
     1021 gcggaactgg tttttgaaaa agaaaaaacc gtcgtcaaag gcaaatccga ccgcaccacc
     1081 atcaccccca actggcgcaa cacctacaaa gtcggcttcg gcggttctta tcaaatcagc
     1141 gaaccgctgc aactgcgcgc cggcatcgct tttgacaaat cgcccgtccg caacgccgac
     1201 taccgcatga acagcctacc cgacggcaac cgcatctggt tctccgccgg tatgaaatac
     1261 catatcggta aaaaccacgt cgtcgatgcc gcctacaccc acatccacat caacgacacc
     1321 agctaccgca cggcgaaggc aagcggcaac gatgtggaca gcaaaggcgc gtcttccgca
     1381 cgtttcaaaa accacgccga catcatcggt ctgcaataca cctacaaatt caaataaacg
     1441 gcccgccgtt tgaatgtaac aatgccgtct gaaacagatt tccgtttcag acggcattgt
     1501 tttaggctcg
//
LOCUS       DAXHSF010000232         1492 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.232, whole genome shotgun sequence.
ACCESSION   DAXHSF010000232 DAXHSF010000000
VERSION     DAXHSF010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.232"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            119..1174
                     /locus_tag="ACFM8S_001998"
     CDS             119..1174
                     /locus_tag="ACFM8S_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFC6315008.1"
                     /translation="MPSETRLPNFIRTLIFALSFIFLNACSEQTAQTVTLQGETMGTT
                     YTVKYLSNNRDKLPSPAEIQKRIDDALKEVNRQMSTYQPDSEISRFNQHTAGKPLRIS
                     SDFAYVTAEAVRLNRLTHGALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGI
                     DKIILKQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELH
                     GKGKNARGEPWRIGIEQPNIIQGGNTQIIVPLNNRSLATSGDYRIFHVDKSGKRLSHI
                     INPNNKRPISHNLASISVVADSAMTADGLSTGLFVLGETEALKLAEREKLAVFLIVRD
                     KGGYRTAMSSEFEKLLR"
     gene            1200..1415
                     /gene="nqrM"
                     /locus_tag="ACFM8S_001999"
     CDS             1200..1415
                     /gene="nqrM"
                     /locus_tag="ACFM8S_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HFC6315009.1"
                     /translation="MKTLLLTFGIFLTVIIGMAVGYIFSKRSIKGSCGGITALGMKKM
                     CDCDTPCDTLQKKLDEEKQAGGIRVDR"
BASE COUNT          425 a          439 c          327 g          301 t
ORIGIN      
        1 taccgccacc taaacgacgg gcgatgccgt ccgaacaccc ttcagacggc atcggcatag
       61 aaacataccg gccgttttgc gatataattc aaaccatttg ccaaaaccgt caaacactat
      121 gccgtctgaa acacgcctgc cgaactttat ccgcaccttg atatttgccc tgagttttat
      181 cttcctgaac gcctgttcgg aacaaaccgc gcaaaccgtt accctgcaag gtgaaacgat
      241 gggcacgacc tataccgtca aatacctttc aaacaatcgg gacaaactcc cctcccctgc
      301 cgaaatacaa aagcgcatcg atgacgcgct taaagaagtc aaccggcaga tgtccaccta
      361 tcagcccgac tccgaaatca gccggttcaa ccaacacaca gccggcaagc ccctccgcat
      421 ttcaagcgac ttcgcatacg ttactgccga agccgtccgc ctaaaccgcc tgacacacgg
      481 cgcgctggac gtaaccgtcg gccctttggt caacctttgg ggattcggcc ccgacaaatc
      541 cgttacccgt gaaccgtcgc cggaacaaat caaacaagca gcatcttata cgggcataga
      601 caaaatcatt ttgaaacaag gcaaagatta cgcttccttg agcaaaaccc accccaaggc
      661 ctatttggat ttatcttcga ttgccaaagg cttcggcgtt gataaagttg cgggcgaact
      721 ggaaaaatac ggcattcaaa attatctggt cgaaatcggc ggcgagttgc acggcaaagg
      781 caaaaacgcg cgcggcgaac cgtggcgcat cggtatcgag caacccaata tcatccaagg
      841 cggcaatacg cagattatcg tcccgctgaa caaccgttcg cttgccactt ccggcgatta
      901 ccgtattttc cacgtcgata aaagcggcaa acgcctctcc catatcatta atccgaacaa
      961 caaacgaccc atcagccaca acctcgcctc catcagcgtg gtcgcagaca gtgcgatgac
     1021 ggcggacggc ttgtccacag gattattcgt attgggcgaa accgaagcct taaagctggc
     1081 agagcgcgaa aaactcgctg ttttcctgat tgtcagagat aaaggcggct accgcaccgc
     1141 catgtcttcc gaatttgaaa aactgctccg ctaaacatac aaaccgatta ggaaacacca
     1201 tgaaaaccct gctccttacc ttcggcatct tcctgaccgt catcatcggt atggcggtcg
     1261 gctatatttt ctccaaacgc agcatcaaag gcagttgcgg cggcattact gccttgggta
     1321 tgaaaaaaat gtgcgactgc gacacacctt gcgacaccct gcaaaagaaa ctggatgaag
     1381 aaaaacaggc aggcggcata cgggttgacc gttaacgccg aaatatagtg gattaacaaa
     1441 aaccagtaca gcgttgcctc gccttagctc aaagagaacg attctctaag gt
//
LOCUS       DAXHSF010000233         1463 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.233, whole genome shotgun sequence.
ACCESSION   DAXHSF010000233 DAXHSF010000000
VERSION     DAXHSF010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.233"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            47..1384
                     /locus_tag="ACFM8S_002000"
     CDS             47..1384
                     /locus_tag="ACFM8S_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFC6315010.1"
                     /translation="MSNHTSWSSKIGFVLAAAGSAIGLGAIWKFPYTAGTNGGAVFFL
                     LFLIFTILVALPVQLAEFYIGRTGGKNAVDSFRVLRPGTQWLWVGRMGVAACFILLSF
                     YSVVGGWVLNYVVHSFTGAVHTGADFEALFGATISNPAGSLSYQALFMLITVWVVKGG
                     ISDGIEKANRYLMPGLFILFIALAIRSLTLPGAMEGVSFLLKPNWSYFKADTMITALG
                     QAFFALSIGVSAMITYASYLGKDQDMFRSGHTIMWMNLLVSLLAGLVIFPAVFAFGFE
                     PSQGPGLIFIVLPAVFMKMPFGTVLFAVFMLLVVFATLTSAFSMLETVIAATIRQDER
                     KRKKHTWLIGTAIFIVGIPSALSFGVWGEFKVFGKTIFDLWDYLISAVIMPVGALSVA
                     VFTAWIQDRQSVLKDAGAGSTVPRAVLLLWLNTLRYLAPIAIIIVFINSLDIL"
BASE COUNT          267 a          345 c          390 g          461 t
ORIGIN      
        1 tttttgttaa tccactatac tatcttcaca tttcttaata aattttatga gtaaccatac
       61 ttcttggtcg tccaaaatcg gtttcgtgct tgctgcggcg ggttcggcca tcggtttggg
      121 cgcgatttgg aaatttcctt atacggcggg caccaacggc ggcgcggtgt ttttcctgct
      181 gtttttgata tttactatct tggtcgccct acccgttcag cttgccgaat tttatatcgg
      241 gcgcacgggc ggtaaaaatg ccgtcgattc cttcagggtt ctgcgtccgg gcacgcaatg
      301 gctttgggtc gggcgtatgg gcgttgccgc ctgctttatt ttgctgtcgt tttacagcgt
      361 ggtcggcgga tgggtattaa attatgtcgt ccacagtttt acgggggcgg ttcataccgg
      421 cgcggacttt gaagccttgt tcggcgcgac gatttccaat ccggcaggtt cgctgtccta
      481 tcaggctctg tttatgctga ttacggtttg ggtggtcaaa ggtgggattt cagacggcat
      541 tgaaaaggca aaccgttatc tgatgccggg gctgtttatc ctctttattg cgctggcaat
      601 ccgttcgctg acgctgccgg gtgcaatgga gggcgtgtca ttcctgctca aaccgaattg
      661 gtcgtacttt aaagccgata cgatgattac ggctttggga caggcgtttt ttgccctgag
      721 catcggtgtt tccgccatga ttacctacgc ttcatatttg ggaaaagatc aggatatgtt
      781 ccgctcgggg catacgatta tgtggatgaa cctcttggtt tcgctgcttg ccggcctggt
      841 gatttttccg gcggtgttcg ccttcggttt tgaaccgagc caggggccgg gattgatttt
      901 tatcgtattg cccgcagtgt ttatgaagat gccgttcggt acggttttgt ttgcggtatt
      961 tatgctgctg gtcgttttcg ccacgctgac ttcggcattt tcgatgttgg aaacggtcat
     1021 tgccgcaacc atccgccaag acgagcgcaa acgcaaaaaa catacttggc ttatcggcac
     1081 ggctattttc attgtcggca tcccatccgc gctgtctttc ggcgtatggg gtgagtttaa
     1141 ggttttcggc aaaaccattt ttgatttgtg ggactacctt atttctgcag tcatcatgcc
     1201 tgtcggcgca ttgagcgttg ccgtatttac cgcttggatt caggacaggc agtctgtgtt
     1261 aaaagatgcc ggcgcgggca gcaccgtacc acgggcagtg ttgctgctgt ggctgaatac
     1321 cttgcgctac ctcgccccga ttgccattat tattgttttc atcaattctt tggacatcct
     1381 ttaaaagcca tccaaacagc aaaaatgccg tctgaaagcc tttcagacgg catttttgct
     1441 tcaggttcag cctatttcgt tca
//
LOCUS       DAXHSF010000234         1441 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.234, whole genome shotgun sequence.
ACCESSION   DAXHSF010000234 DAXHSF010000000
VERSION     DAXHSF010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.234"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            72..1247
                     /gene="porA"
                     /locus_tag="ACFM8S_002001"
     CDS             72..1247
                     /gene="porA"
                     /locus_tag="ACFM8S_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin PorA"
                     /protein_id="HFC6315011.1"
                     /translation="MRKKLTALVLSALPLAAVADVSLYGEIKAGVEGRNIQAQLTEQP
                     QVTNGVQGNQVKVTKAKSRIRTKISDFGSFIGFKGSEDLGEGLKAVWQLEQDVSVAGG
                     GASQWGNRESFIGLAGEFGTLRAGRVANQFDDASQAINPWDSNNDVASQLGIFKRHDD
                     MPVSVRYDSPEFSGFSGSVQFVPAQNSKSAYKPAYYTKDTNNNLTLVPAVVGKPGSDV
                     YYAGLNYKNGGFAGNYAFKYARHANVGRNAFELFLIGSATSDEAKGTDPLKNHQVHRL
                     TGGYEEGGLNLALAAQLDLSENADKTKNSTTEIAATASYRFGNAVPRISYAHGFDFIE
                     RGKKGENTSYDQIIAGVDYDFSKRTSAIVSGAWLKRNTGIGNYTQINAASVGLRHKF"
BASE COUNT          370 a          354 c          368 g          349 t
ORIGIN      
        1 ataattgaag acgtatcggg tgtttgcccg atgtttttag gtttttatca aatttacaaa
       61 aggaagccga tatgcgaaaa aaacttaccg ccctcgtatt gtccgcactg ccgcttgcgg
      121 ccgttgccga tgtcagcctg tacggcgaaa tcaaagccgg cgtggaaggc aggaacatcc
      181 aggcgcaatt gaccgagcag ccccaagtaa ctaacggtgt gcaaggcaat caggtaaaag
      241 ttactaaggc caaaagccgc atcaggacga aaatcagcga tttcggctcg tttatcggct
      301 ttaaggggag cgaggatttg ggcgaagggc tgaaggctgt ttggcagctt gagcaagacg
      361 tatccgttgc cggcggcggc gcgtcccagt ggggcaacag ggaatccttt atcggcttgg
      421 caggcgaatt cggtacgctg cgcgccggtc gcgttgcaaa tcagtttgac gatgccagcc
      481 aagccattaa tccttgggac agcaataatg atgtggcttc gcaattgggt attttcaaac
      541 gccacgacga tatgccggtt tccgtacgct acgattctcc ggaattttcc ggtttcagcg
      601 gcagcgtcca attcgttccg gctcaaaaca gcaagtccgc ctataagccg gcttattata
      661 ctaaggatac aaacaataat cttactctcg ttccggctgt tgtcggcaag cccggatcgg
      721 atgtgtatta tgccggtctg aattacaaaa atggcggttt tgccgggaac tatgccttta
      781 aatatgcgag acacgccaat gtcggacgta atgcttttga gttgttcttg atcggcagcg
      841 cgacgagtga tgaagccaaa ggtaccgatc ccttgaaaaa ccatcaggta caccgcctga
      901 cgggcggcta tgaggaaggc ggcttgaatc tcgccttggc ggctcagttg gatttgtctg
      961 aaaatgccga caaaaccaaa aacagtacga ccgaaattgc cgccactgct tcctaccgct
     1021 tcggtaatgc agttccgcgc atcagctatg cccatggttt cgactttatc gaacgcggta
     1081 aaaaaggcga aaataccagc tacgatcaaa tcatcgccgg cgttgattat gatttttcca
     1141 aacgcacttc cgccatcgtg tctggcgctt ggctgaaacg caataccggc atcggcaact
     1201 acactcaaat taatgccgcc tccgtcggtt tgcgccacaa attctaaata gcggggcggt
     1261 gaagcggata gctttgtttt tgacggcttg ccttcattct ttgattgcaa tctgactgcc
     1321 aatctgcttc agccccaaac aaaaatccgg atacggaaga aaaacggcaa taaagacagc
     1381 aaataccgtc tgaaagattt tcagacggta tttcgcattt tcggcttggt ttgcacatat
     1441 a
//
LOCUS       DAXHSF010000235         1440 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.235, whole genome shotgun sequence.
ACCESSION   DAXHSF010000235 DAXHSF010000000
VERSION     DAXHSF010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.235"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(<1..138)
                     /locus_tag="ACFM8S_002002"
     CDS             complement(<1..138)
                     /locus_tag="ACFM8S_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC6315012.1"
                     /translation="MSTFFQQTAQAMIAKHINRFPLLKLDRVIDWQPIEQYLNRQKTR
                     YL"
     gene            477..745
                     /locus_tag="ACFM8S_002003"
                     /pseudo
     CDS             477..745
                     /locus_tag="ACFM8S_002003"
                     /inference="COORDINATES: protein motif:HMM:NF017953.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
     gene            complement(835..1284)
                     /locus_tag="ACFM8S_002004"
     CDS             complement(835..1284)
                     /locus_tag="ACFM8S_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HFC6315013.1"
                     /translation="MTDNRGFTLVELISVVLILSVLALIVYPSYRNYVEKAKINAVRA
                     ALLENAHFMEKFYLQNGRFKQTSTKWPSLPIKEAEGFCIRLNGIARGALDSKFMLKAV
                     AIDKDKNPFIIKMNENLVTFICKKSASSCSDGLDYFKGNDKDCKLFK"
     gene            complement(1372..>1440)
                     /locus_tag="ACFM8S_002005"
     CDS             complement(1372..>1440)
                     /locus_tag="ACFM8S_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HFC6315014.1"
                     /translation="QASVGAPAPKAPAKPAIPAAKK"
BASE COUNT          449 a          309 c          255 g          427 t
ORIGIN      
        1 aaggtaacgg gttttttgac ggttcaggta ctgctcgatc ggctgccaat caattacccg
       61 gtccaacttc aatagcggga agcggttgat gtgtttggca atcatggctt gggcggtttg
      121 ctggaagaag gtgctcatga gaaatctcct aaatgtcttg gtgggaattt aggggatttt
      181 ggggaatttt gcaaaggtct ccttatgtct aaaggtgcaa acatgcctta aattaccata
      241 gtggattaaa tttaaatcag gacaaggcga ggcaacgctg tactggttta aatttaatcc
      301 actatatatt gcataggcaa aatacaagcc tataacgaat tggaaacaaa atgccgtctg
      361 aaaacatctt cagacggcat tataaaatct gttcaccttt tcagatgagt aatgtacacc
      421 cttatacaat ttttgctact atgccccata aatccacggc taaagatatc cttattatgt
      481 cctatgattt atcgaaacga cttgtaatcg gcttagcatc aagtgcccta ttcgacttat
      541 ccgaatcgga taatatattt agaatggaag gggcagaaac ctataggcaa tatcagaggg
      601 aaaaacaaaa ccatccccta aaaaaggcgt tgtctttcca tttattaaaa aacttctgtc
      661 aatcaatgaa ataaacccaa acgacccaac gattgggttt attcttttat ccagaaacaa
      721 tccagataca gattacgagt cataactata ggcttaatat tacacgattt tcattccatc
      781 aaggcggaaa accgcacaaa tactgaaaca ctatcgatcg atttgtaaac aagcctactt
      841 aaataacttg cagtccttat catttccttt aaaataatcc agcccgtcac tacacgaact
      901 ggcggacttc ttgcaaataa aggttactag attttcattc atcttaataa taaaaggatt
      961 tttatcttta tctatggcta ccgccttcaa catgaattta ctgtctaaag ccccgcgcgc
     1021 gattccattc aaacggatac aaaagccttc tgcctcttta atcggcaaac ttggccattt
     1081 ggtagatgtt tgtttaaacc tcccattctg cagataaaac ttttccataa aatgtgcatt
     1141 ttctaacaag gctgcccgca ctgcatttat ctttgctttc tcaacataat tgcgatagct
     1201 cggataaaca attaaagcaa gtacagacaa tatcaagacc actgatatta attcaaccag
     1261 cgtaaacccc cgattatcag tcattacttt acttccaata agaacagatt attcaacata
     1321 tttctttgaa cagacttact atcccattca acagtatgca tatttcccac tctatttttt
     1381 agcggccggt atagccggtt tggctggggc ttttggtgcg ggcgcgccga ccgaagcctg
//
LOCUS       DAXHSF010000236         1429 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.236, whole genome shotgun sequence.
ACCESSION   DAXHSF010000236 DAXHSF010000000
VERSION     DAXHSF010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.236"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..359
                     /locus_tag="ACFM8S_002006"
     CDS             <1..359
                     /locus_tag="ACFM8S_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFC6315015.1"
                     /translation="VLIFVNPLYIIATTVPAQQAFLDAAELMQWSIETEGLGLNVISH
                     KILGKDHAQVEFEAYFRDGQHRSAHHELSGFVNIGGQWYFIDPTVPHPAMKQPCICGS
                     GKKFKACCGKYLKPVA"
     gene            complement(578..1372)
                     /locus_tag="ACFM8S_002007"
     CDS             complement(578..1372)
                     /locus_tag="ACFM8S_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFC6315016.1"
                     /translation="MLHRLQKVVRHIAQTEIMPRFLNTPSHRKEDGSMLSEADIAAQT
                     AFAAALPLLIDSPMLGEEMSRQEQSALWEQYSGEKGLWIVDPIDGTNNFVNGLPHFAV
                     SVAFVRNGRAELGVIYNPVSGECFYAERGQGAFLNGTRLPLRLVDKKLNEAIAGVEIK
                     YLRSGKLSSRMSTLAPFGTIRSMGSSTLDWCYLACGRYDVYVHGGQKLWDYAAGALIF
                     EEAGGRLTTLEGDGFWSGEHVFKRSVVAALEPKLFERWVGWIRENQ"
BASE COUNT          391 a          425 c          305 g          308 t
ORIGIN      
        1 ttgtcctgat ttttgttaat ccactataca tcatcgctac taccgttccg gcgcaacagg
       61 cattcctcga tgccgccgaa ctgatgcaat ggagtataga aaccgaaggg ctgggcttga
      121 acgtcatctc gcacaagata ctcggcaaag accacgccca agtcgaattt gaagcctact
      181 tccgagacgg acaacaccga tccgcgcatc acgaactgtc cggcttcgtc aacatcggcg
      241 gacaatggta ttttatcgat cccaccgttc cgcatcctgc gatgaaacaa ccctgcattt
      301 gcggatcagg caaaaaattc aaagcctgct gcggcaaata tctgaaacct gtcgcataaa
      361 aatgccgtct gaacgttcag acggcatttt caacgtgcaa aaaaaaacca ttcataccaa
      421 gggtaagtat gaatggtcaa tacattgcgg gaaaacgtct tacttgctgc actgccgaaa
      481 agggagaaac ggcagcggta atcagcgaaa aggattgtac ccgaattaat gttaagaaac
      541 gttaatcgcg aaaatatatt aacaaacctg ttgaaaccta ttggttttcc cgtatccacc
      601 cgacccagcg ttcaaacagc ttcggttcga gcgcggcaac gaccgagcgt ttgaacacgt
      661 gttcaccact ccaaaacccg tcgccttcca aagtcgtcag cctgccgccc gcctcctcaa
      721 aaatcagcgc acccgcagca tagtcccaca gcttctgccc accgtgaaca taaacatcat
      781 aacgcccgca cgccagataa caccaatcca acgtactgct gcccatactc cgtatcgttc
      841 caaaaggcgc gagcgtactc atacggctgg aaagtttgcc cgaacgcaaa tatttgattt
      901 ccacgcccgc aatcgcctca ttgagttttt tatccacgag acgcaaaggc aggcgggttc
      961 cattaagaaa cgcaccctgc ccccgttcgg cataaaaaca ttcgccgctg accggattgt
     1021 aaatcacacc caattcggcg cgcccgttgc ggacaaacgc caccgacacg gcaaaatgcg
     1081 gcagcccgtt gacaaaattg ttcgtcccgt ctatcggatc gacaatccac agcccctttt
     1141 cccccgaata ttgttcccac aaagccgact gttcctgccg cgacatttcc tcgcccaaca
     1201 tcggactgtc gattaaaagc ggcaacgcgg cggcaaaagc cgtctgcgcg gcaatgtccg
     1261 cctcgctcaa catcgaaccg tcttccttgc ggtgagacgg cgtattcaaa aaacgcggca
     1321 taatttcggt ttgcgcgata tggcgcacga ctttctgcaa acggtgtaac acttcctact
     1381 gtcctcatat tttgaacttg cggcacgcga acgtatatag tggattaaa
//
LOCUS       DAXHSF010000237         1407 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.237, whole genome shotgun sequence.
ACCESSION   DAXHSF010000237 DAXHSF010000000
VERSION     DAXHSF010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.237"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..876
                     /locus_tag="ACFM8S_002008"
     CDS             <1..876
                     /locus_tag="ACFM8S_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 49 family protein"
                     /protein_id="HFC6315017.1"
                     /translation="GNTANFNSGCPEAIDYVDERKGVPLAAMRHILMYTEAAASHAYL
                     FEHDLNKFKQYAYVAGKLGVLLSVNAEDPEPFFFPCDMLNIQNPMFLMLMSDSPQLRE
                     FLVRNIDNIANDTEAFVNRYDLNRHMIYNTLLMVEGKQLDRLKQRSEKVLVHPTPSKW
                     LQKRLYDYRFFLAFAEQDAEAMKAALEPLFDKKTARMAAKETLSYFDFYLQPQIVTYA
                     KIASMHGFDLGIDHEIAPRDLTVYDPLPADEYQDIFDFMKQYDLSYPYEYLQDWIDYY
                     TFKTDKLVFGNAKRE"
BASE COUNT          387 a          347 c          307 g          366 t
ORIGIN      
        1 ggcaatactg ctaatttcaa tagcggttgc cctgaagcta tagattatgt agatgaaaga
       61 aaaggcgttc cgcttgcagc gatgagacat attttaatgt atactgaagc tgcggcttcc
      121 catgcctatc tatttgaaca tgatcttaat aaattcaagc aatatgctta tgttgcagga
      181 aagctggggg ttttgctgag tgtaaatgcc gaagaccccg aacccttctt ctttccctgt
      241 gacatgctca acattcaaaa tccgatgttt ctgatgctga tgagcgacag cccgcagctg
      301 cgtgagtttc tggtgcgcaa tatcgacaac atcgccaacg atacagaagc cttcgtaaac
      361 cgctacgacc tcaaccggca tatgatttac aatactctgc tgatggtgga aggtaagcag
      421 cttgatcggt tgaaacaacg tagcgagaaa gtcttggtgc accccacccc tagcaaatgg
      481 ctgcaaaagc ggttgtacga ttaccgcttc ttcctcgctt tcgccgaaca ggatgccgag
      541 gcgatgaagg ccgccttaga gccgcttttc gataaaaaaa ccgcgcgtat ggctgccaaa
      601 gaaacattgt cctatttcga tttctacctg cagccgcaaa tcgttaccta cgccaaaatc
      661 gcatccatgc acggtttcga tttgggcata gaccacgaaa tcgcgccgag ggatttgact
      721 gtttacgatc cgctgccggc agacgaatat caagacatct tcgattttat gaaacagtat
      781 gacttgtctt acccgtatga atatctgcag gattggatag attactatac gttcaaaacc
      841 gataagctgg tatttggtaa cgcgaagcga gagtgagccg taaaactctg agctcctgtt
      901 ttatagatta caactttagg ccgtcttaaa gctgaaagat tttcgaaagc tataaattga
      961 agcccttcca cagtacatag atctgtgttg tggcggggct ttaccacgct gattgccgga
     1021 gaagaactca acctgctggc aaaacaaggc atgagatctt tgcaataaca tgagttgaga
     1081 cctttgcaaa aaagcccttc cccgacatcc gaaacccaaa cacaggattt cggctgtttt
     1141 cgtaccaaat acctcctaat tttacccaaa tatcccctta atcctccccg gatacccgat
     1201 aatcaggcat ccgggctgcc ttttaggcgg cgcgggcgca cttagcctgt tggcggcctt
     1261 caacaggttc aaacacaccg ccttcagatg gtttgcgcac tcactttgtc atttccaatg
     1321 ttccaagtac acctaatctg ctaagaggaa gacagcttga aggaaaactt attttagaag
     1381 ttcctgctca ggtcaatcca attccac
//
LOCUS       DAXHSF010000238         1379 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.238, whole genome shotgun sequence.
ACCESSION   DAXHSF010000238 DAXHSF010000000
VERSION     DAXHSF010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.238"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..921
                     /gene="mafA"
                     /locus_tag="ACFM8S_002009"
     CDS             <1..921
                     /gene="mafA"
                     /locus_tag="ACFM8S_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HFC6315018.1"
                     /translation="IPLVLTACGTLTGIPAHGGGKRFAVEQELVAASSRAAVKEMDLS
                     ALKGRKAALYVSVMGDQGSGNISGGRYSIDALIRGGYHNNPESATQYSYPAYDTTATT
                     KSDALSSVTTSTSLLNAPAAALTKNSGRKGERSAGLSVNGTGDYRNETLLANPRDVSF
                     LTNLIQTVFYLRGIEVVPPEYADTDVFVTVDVFGTVRSRTELHLYNAETLKAQTKLEY
                     FAVDRDSRKLLIAPKTAAYESQYQEQYALWTGPYKVSKTVKASDRLMVDFSDITPYGD
                     TTAQNRPDFKQNNGKNPDVGNEVIRRRKGG"
     gene            925..>1379
                     /gene="mafB"
                     /locus_tag="ACFM8S_002010"
     CDS             925..>1379
                     /gene="mafB"
                     /locus_tag="ACFM8S_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HFC6315019.1"
                     /translation="MKPLRRLTNLLAACAVAAAAFGQPALAADLAQDPFITDNTQRQH
                     YEPGGKYHLFGDPRGSVSDRTGQINVTQDYTHQMGNLLIQQANINGTIGYHTRFSGHG
                     HEEHAPFDNHAADSASKEQGNVDDGFTVYRLNWEGHEHHPADAYDGPKGG"
BASE COUNT          353 a          525 c          305 g          196 t
ORIGIN      
        1 atccccctcg tcctaaccgc ctgcggcaca ctgaccggca tacccgccca cggcggcggc
       61 aaacgctttg ccgtcgaaca ggaactcgtc gccgcatcgt cccgcgccgc cgtcaaagaa
      121 atggacttgt ccgccctgaa aggacgcaaa gccgcccttt acgtctccgt tatgggcgac
      181 caaggttcgg gcaacataag cggcggacgc tactctatcg acgcactgat acgcggcggc
      241 taccacaaca accccgaaag tgccacccaa tacagctacc ccgcctacga cactaccgcc
      301 accaccaaat ccgacgctct ctccagcgta accacttcca catcgctttt gaacgccccc
      361 gccgccgccc tgacgaaaaa cagcggacgc aaaggcgaac gctccgccgg actgtccgtc
      421 aacggcacgg gcgactaccg caacgaaacc ctgctcgcca acccccgcga cgtttccttc
      481 ctgaccaacc tcatccaaac cgtcttctac ctgcgcggca tcgaagtcgt accgcccgaa
      541 tacgccgaca ccgacgtatt cgtaaccgtc gacgtattcg gcaccgtccg cagccgcacc
      601 gaactgcacc tctacaacgc cgaaaccctt aaagcccaaa ccaagctcga atatttcgcc
      661 gtcgaccgcg acagccggaa actgctgatt gcccctaaaa ccgccgccta cgaatcccaa
      721 taccaagaac aatacgccct ttggaccggc ccttacaaag tcagcaaaac cgtcaaagcc
      781 tcagaccgcc tgatggtcga tttctccgac atcaccccct acggcgacac aaccgcccaa
      841 aaccgtcccg acttcaaaca aaacaacggt aaaaaccccg atgtcggcaa cgaagtcatc
      901 cgccgccgca aaggaggata aaccgtgaaa ccgctgcgca gactgacaaa cctccttgcc
      961 gcctgcgccg tagcggccgc tgctttcgga cagcccgccc tcgcggcgga cttggcgcaa
     1021 gacccgttca ttaccgataa cacccaacgg cagcactacg aacccggagg caaataccac
     1081 ctcttcggcg acccgcgcgg cagcgtttcc gaccgcaccg gtcaaatcaa cgtcacccaa
     1141 gactataccc accagatggg caacctgctc atccaacagg caaacatcaa cggcacaatc
     1201 ggctaccaca cccgcttttc cggacacgga cacgaagaac acgccccctt cgacaaccac
     1261 gccgccgaca gcgcaagcaa agaacaaggc aacgttgacg acggctttac cgtgtatcgg
     1321 ctcaactggg aaggacacga acatcatccc gccgatgcct acgacggccc gaagggcgg
//
LOCUS       DAXHSF010000239         1279 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.239, whole genome shotgun sequence.
ACCESSION   DAXHSF010000239 DAXHSF010000000
VERSION     DAXHSF010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.239"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            57..782
                     /locus_tag="ACFM8S_002011"
     CDS             57..782
                     /locus_tag="ACFM8S_002011"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFC6315020.1"
                     /translation="MPRFYLPENLSVGQTVALPDNIVRHLNVLRVRPNENITLFDGKG
                     KAHAARLTVLEKRRAEAEILHEDTTDNESPLNITLIQSISSGDRMDFTLQKSVELGVT
                     AIQPVISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKM
                     PSESTKLIMSINRARKLGDIRHPSGAIVFMVGPEGGWTEQEEQQAFDAGFQTVTLGKR
                     ILRTETVALAALAAMQTLWGDFA"
BASE COUNT          345 a          401 c          289 g          244 t
ORIGIN      
        1 gtccgtttcc atcacgccgc tgcgacagat tataaccgtc cgaaccgcca aaaactatgc
       61 cccgattcta cctgcccgaa aacctttccg tcggacaaac cgtcgccctg cccgacaaca
      121 tcgtccgcca cctcaacgtc ctacgcgtcc gccccaacga aaacatcacc ctcttcgacg
      181 gcaaaggcaa ggcacacgcc gcacggctga ccgttttgga aaaacgccgc gccgaagccg
      241 aaatcctgca cgaagacaca accgacaacg agtccccgct caacatcaca ctgatacaat
      301 ccatctcctc cggcgatcgc atggatttca ccctgcaaaa aagcgtcgaa ctcggcgtaa
      361 ccgccataca gcccgtcatc agcgaacgct gcatcgtccg cctcgatggg gaacgcgccg
      421 ccaaacgcct cgcacgctgg caggaaatcg tcatctccgc gtgcgaacaa agcggcagga
      481 acaccgttcc ccccgtactg cccatcatcg gctaccgtga agcactcgac aaaatgccgt
      541 ctgaaagcac caagctgatt atgagcatca accgcgcccg caaactcggc gacatccgcc
      601 acccgtccgg cgcaatcgtc tttatggtcg ggcccgaagg cggctggaca gaacaggaag
      661 aacaacaggc atttgatgcc ggcttccaaa ccgtaacatt gggcaaacgc atcctgcgca
      721 ccgaaaccgt cgccctcgcc gccctcgccg ccatgcagac gctttggggc gatttcgcat
      781 aaacagaaat gccgtctgaa acccgttcag acggcatttt gcagccgatt aagatagtag
      841 gttcaaataa gatttccccc gccgtcattc ccgcgaaagc aagaatctag aaacgaaaaa
      901 ctacagggat ttatccgaaa caacaaaccc tctccgccgt cattcccgcg caggcgggaa
      961 tctagaaacg aaaaactaca gggatttatc cgaaacaaca aaccctctcc gccgtcattc
     1021 ccgcgaaagc gggaatctag aaatttaacg ttgcggtgat ttatcggaaa tgactgaaac
     1081 tcaacggact ggattcccgc ctgcgcggga atgacgagat tttaggtttc tgtttttggt
     1141 tttctgttct cgtgggaata acggaatttt aagttttagg aatttgtcgg aaaaacagaa
     1201 atccccccgc cgtcattctc gcaaaagcgg gaatctggaa atttaatgtt gcggtgattt
     1261 atcggaaatg actgaacct
//
LOCUS       DAXHSF010000240         1267 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.240, whole genome shotgun sequence.
ACCESSION   DAXHSF010000240 DAXHSF010000000
VERSION     DAXHSF010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.240"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(34..492)
                     /locus_tag="ACFM8S_002012"
     CDS             complement(34..492)
                     /locus_tag="ACFM8S_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6315021.1"
                     /translation="MLTKLKILLFLFLFVFVLAINLLFFFFSSDIESFGNYQFEYVYD
                     KGWPANYILVMKDGNEGNFDKIISGLVLEYYKEDDNIYFSYIDGQGFASDSCYYKPEI
                     LYGKIILNKNHIININSMEKNNFLSEDKIMKGTRNWLADPKNKCNIQTLD"
     gene            complement(1056..>1267)
                     /locus_tag="ACFM8S_002013"
     CDS             complement(1056..>1267)
                     /locus_tag="ACFM8S_002013"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6315022.1"
                     /translation="KKMLSLEIQQEGFTIGGGLVLGGCAGAHLARKEPLILTGKTGAG
                     ASAIANASIGYQWTVNLSKPKEGAK"
BASE COUNT          448 a          229 c          132 g          458 t
ORIGIN      
        1 caatcgattc cgttgtattt tcgcaagaca cgtttagtct agagtctgta tattacattt
       61 atttttaggg tctgctagcc aatttcttgt tcccttcatt attttatctt ctgaaagaaa
      121 attatttttt tccatgctat taatattaat gatatgattt ttatttaaaa taatttttcc
      181 atataaaatt tccggtttgt aatagcaaga gtctgaagca aatccttgcc cgtcaatata
      241 agaaaaataa atgttatcat cctccttata atattctaaa accaatccgg atattatttt
      301 atcaaaattc ccttcatttc catctttcat gactaaaata taattagcag gccaaccttt
      361 atcgtaaaca tattcaaact gatagttccc gaaactctcg atatccgaac taaaaaagaa
      421 gaaaagcaaa ttaatagcca atacaaaaac aaataagaac aaaaatagca aaattttcaa
      481 cttagttaac aatatttacc tctcctttaa attcaatcct gaaaggtacc ccttaccttg
      541 ggctaccaat tatagtttcc atatttctaa atattgtttt tacattactt ttttctcccc
      601 ccaaaggaat gcattttaaa atcatgcttt tcaggtgcta atcgatactt accattaaca
      661 tctttaatca cagatatatt tccatgtata gcccaacgtg aaaaatctga gtattatata
      721 cagttatacc ttactgaaac aaagctagct gtacatatct gtaccatatt tttactaaaa
      781 acaaaaaata tttataatta ttaaggataa gataaaaacc cccaacgcca gattaattct
      841 taataatttc aaataaaaaa tatttgagat tttttatttt ccaaataata tattgaaata
      901 tttaaagtta aggtagcttg atttaattag aaaatcaaaa aaaccattaa ttaatttttc
      961 ttctttaaat attttatatg atattataaa attaaataac attgcgaaaa catatgaaat
     1021 catcaaaata ccaaatatat agtgggaatg cattattatt tagctccttc ttttggcttt
     1081 gacaaattga cagtccattg atatccaatg cttgcatttg caattgctga cgcacctgcc
     1141 cctgttttcc ctgttagtat caatggttct tttcttgcaa gatgtgcacc tgcacaacca
     1201 cctaatacta aaccaccacc tattgtaaat ccttcctgtt gtatctctaa tgataacatt
     1261 ttcttct
//
LOCUS       DAXHSF010000241         1252 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.241, whole genome shotgun sequence.
ACCESSION   DAXHSF010000241 DAXHSF010000000
VERSION     DAXHSF010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.241"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            235..1188
                     /locus_tag="ACFM8S_002014"
     CDS             235..1188
                     /locus_tag="ACFM8S_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFC6315023.1"
                     /translation="MGFLIGLSRNLTRFTALVILLASAVAFIGPASFAWVKGDVQVLV
                     LGIIMLGMGMTLGREDYRILAQRPLDVFIGAAAQYTVMPLLAIGIAKLFDLSPGLTLG
                     LVLVGTCPGGVSSNIMSFLAKGDVAFSVGMTTVSTVLAPAVTPLWMTCLVGQTVDMDG
                     WGMFRFMLLVTLLPVVLGSAANILLHGKHWFENVRAVMPGVAVLAFACIVGGVASVHG
                     HRFAESALVMVLAIAVHNIAGYVSGYYAAALFGMDTAKKRTLAIEVGVQNAGLATGLS
                     AKFFPGNAESAVAAAVACVWHSVSGTVLGNLFALWDKRRDA"
BASE COUNT          197 a          268 c          435 g          352 t
ORIGIN      
        1 aaccggatga ttcgggctaa ataattcctg attctttgga tatagccaac ggtatgcctg
       61 ctgatgccgt cagaaagggc ttgatgggcg tttgcggttc gggttaatat ttgtgctgta
      121 tctgttaatt ccggttagta aaatggggcg gggacgatga gtggccgtcg gtcggttttg
      181 cacgggcggt aataatgtcc ggcgcaaggt gcgccgtttc gggaggggtg tgtgatgggt
      241 tttctgattg gtttgagccg caacctgacg cggtttacgg ctttggtcat tttgcttgcg
      301 tcggcggttg cgtttatcgg accggcttcg tttgcgtggg ttaagggcga tgttcaggtc
      361 ttggtgctgg gcatcattat gctgggtatg gggatgactt tgggcaggga ggactaccgg
      421 attttggcgc agcgtccttt ggatgtgttt atcggtgcgg cggcgcaata taccgtgatg
      481 ccgttgctcg ccatcggtat tgccaagctg ttcgaccttt cgccggggct gactttgggg
      541 ctggtgttgg tggggacgtg tccgggcggc gtgtcgtcca acattatgag tttccttgcc
      601 aaaggcgatg tggcgttttc agtcggtatg acgacggtgt ctacggtgct ggctccggcc
      661 gttacgcctt tgtggatgac ctgcctggtc ggtcagacgg tggatatgga cggttggggg
      721 atgttccggt ttatgctgct ggtcacgctt ttgccggtgg tgctgggttc ggcggcaaat
      781 atcctgctgc acggaaaaca ttggtttgaa aatgtccgtg ccgttatgcc gggcgtggcg
      841 gtattggcat ttgcctgcat tgtcggcggt gtggcttcgg ttcacggaca ccgttttgcc
      901 gaatcggcgt tggtaatggt gttggcgatt gcggtacaca atatcgccgg ctatgtgtca
      961 ggctattacg ccgccgcgct gtttggtatg gatacggcaa aaaaacgcac cttggcaatc
     1021 gaagtcggcg tgcagaatgc cggtttggca acgggattga gcgcgaaatt ttttccgggc
     1081 aacgccgaat cggcggttgc ggcggcggtc gcctgcgtgt ggcattcggt ttcggggacg
     1141 gtattgggca acttgttcgc tctgtgggac aagcgtcggg acgcttagtc gggcgtgtcc
     1201 gaaccgtccc acgatatata gtggattaac tttaaaccgg tacggcgttg cc
//
LOCUS       DAXHSF010000242         1222 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.242, whole genome shotgun sequence.
ACCESSION   DAXHSF010000242 DAXHSF010000000
VERSION     DAXHSF010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.242"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            714..938
                     /locus_tag="ACFM8S_002015"
                     /pseudo
     CDS             714..938
                     /locus_tag="ACFM8S_002015"
                     /inference="COORDINATES: protein motif:HMM:NF044726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            complement(1106..1222)
                     /locus_tag="ACFM8S_002016"
                     /pseudo
     CDS             complement(1106..1222)
                     /locus_tag="ACFM8S_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          428 a          218 c          261 g          315 t
ORIGIN      
        1 ggcgacgata ccgatatttc caagtttgtg tatcaaaatt gtatatcggg catagactat
       61 ttcggcgcgg acgaagatat agatttcccc gactcaccga acataggcta aaaaccaagg
      121 tgtttttcaa tacctaaaaa gtatcctaaa ttattcagga gatgtaataa aggtttgcgg
      181 tttcgtataa ggaaaatcag aatttcacgc cgacacggac gccgtattcg ttctaacatc
      241 aagaaactct gtgtattagt gcataataga gagatatagt agccacatga aatggaaatc
      301 aaaatgacat caaaccatat tgataaacaa aaaacaccgc aatccttaat ccacatgggc
      361 cattccttga ctgaaatgca atacaagtat tggtatcttt ttttgcagaa gattaaaaca
      421 cattttgaat aaggtggtac ttatgatgac aacggttttg ttgcgataga tatcttagat
      481 ctggaacgtt gtattggata tcaacttcaa agaaaacaac taaaaaagac ttggatgctc
      541 tacgaatggc atcaatcatt ttgaattatt tggagaaaga cggccgtccc gtcatacacg
      601 gtatgggcta tatttcagag tggaagatca acaatggcaa agttttctac aatatatcag
      661 acaccattct taatgagtta ctattgaaaa gcggaagaag catgtttttg ctgatggatt
      721 ggagtgtgtt caatagctta ccgggaaaat attgcggaat cctatacaaa ttgtgcaagg
      781 attacgcagg catcggcaaa accccctatt ttaccgttca agaatttaga cactatttgg
      841 gattggatga caaagaatac gaagaattta gagcgttgaa ggccaaaata attaaaccgg
      901 caatagagca tatcaacaac agtgaaaatg tggacatcaa aattataccg caattaaaac
      961 gggaaaatag gcgggttgtg ggactccatt ttgatattga atacaaaaca aatatagaaa
     1021 aaaacaacca gaacatggca gacgaggata tagaaacagc cttttctgaa tcgaaaatag
     1081 agccgctagc cacactgatt aaatttctgg aaaccaaaac accacgttcg agttcgggat
     1141 tggagaggct gtgccattgt ccgagcagaa cggctttgaa catggacaac agcgggtagg
     1201 cgggacggcc gcggtggtct ct
//
LOCUS       DAXHSF010000243         1175 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.243, whole genome shotgun sequence.
ACCESSION   DAXHSF010000243 DAXHSF010000000
VERSION     DAXHSF010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.243"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..408
                     /gene="mafB"
                     /locus_tag="ACFM8S_002017"
     CDS             <1..408
                     /gene="mafB"
                     /locus_tag="ACFM8S_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HFC6315024.1"
                     /translation="GDFSDSYKHNTASRLSQSVDGEMFQTRNVDFKAKSIGTKIHDGA
                     QGKHISGHRNYIEGKSTLNQNINPQELLNGIHSGAYPVISKGARGNPVVDFGYPIGSD
                     GKSGLSTNFGTIHSGKNGVHIVPANPKTIKKVQ"
     gene            411..728
                     /locus_tag="ACFM8S_002018"
     CDS             411..728
                     /locus_tag="ACFM8S_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6315025.1"
                     /translation="MNILPSWLRVGMNIAMLGMIHSDIRLITVDYEEGRRFLKIKNYL
                     SREAITEDHEDMEYLITELWSMCGEYFDEADFECIYSNHSSMELNQINGAVFRRKELI
                     SQA"
     gene            892..>1175
                     /locus_tag="ACFM8S_002019"
     CDS             892..>1175
                     /locus_tag="ACFM8S_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin-type HINT domain-containing
                     protein"
                     /protein_id="HFC6315026.1"
                     /translation="MARIRTGDRVFAKDEASGKTGYKPVTARYGNPYQETVYIEISDG
                     IGKIQTLVSNKIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITLKQ"
BASE COUNT          400 a          223 c          244 g          308 t
ORIGIN      
        1 ggggatttct cagactccta caagcataac actgcttcaa gattatctca gtctgtagat
       61 ggagaaatgt ttcaaacccg caatgttgat tttaaagcaa aatctattgg gactaaaatt
      121 catgatggag ctcaagggaa acatatttca ggacatagaa actacattga aggtaagagt
      181 actttaaatc aaaacattaa tcctcaagaa ttgttgaacg gaatacattc aggtgcttat
      241 ccagttattt ctaaaggagc aagaggaaat cctgttgttg attttgggta tcctataggc
      301 agcgatggga aatcaggatt aagtaccaat tttggtacga ttcattcagg taaaaatgga
      361 gttcacattg ttccggctaa ccctaaaacc attaaaaagg tgcaatagtt atgaatatat
      421 taccaagctg gctgcgagtc ggtatgaata tagcaatgct gggcatgata cactcagata
      481 tcaggttaat taccgtagat tacgaggaag gaagaaggtt tttaaaaatc aaaaattatt
      541 tatcaagaga agccatcaca gaagaccatg aagatatgga atatttgatt acagagttat
      601 ggtctatgtg tggagaatat tttgatgaag ctgactttga atgtatttat tctaatcatt
      661 cttctatgga gttaaaccaa ataaatggtg cagtattcag gagaaaggaa ttaatttcgc
      721 aagcgtaggt taaaaaacca acaatcacaa tgtcttctga aaccgtgttt aattttcaga
      781 cggcatttcc ttcgtttgaa ataggatatt gagaactgag ttcttcaaaa atcctacact
      841 tgctccttcc acggcagcac cttggtcaaa acggcagacg gctgaaaagc cattgcccgt
      901 atccgaaccg gcgaccgcgt cttcgccaag gacgaggcaa gcggaaaaac gggatacaaa
      961 cccgttaccg cccgatacgg caatccgtat caagaaaccg tttacattga aatttcagac
     1021 ggcatcggta aaatacagac cctcgtttcc aataaaatcc acccgtttta cagtcaagga
     1081 aagtggatac aggcaggtcg tctgaaaaaa ggcgacaccc tgctttccga aagcggtgca
     1141 aaacagacgg ttcaaaacat taccctcaaa cagca
//
LOCUS       DAXHSF010000244         1173 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.244, whole genome shotgun sequence.
ACCESSION   DAXHSF010000244 DAXHSF010000000
VERSION     DAXHSF010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.244"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..588
                     /locus_tag="ACFM8S_002020"
     CDS             <1..588
                     /locus_tag="ACFM8S_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6315027.1"
                     /translation="TQAAEAGAGIATGAVTVGNAWEAPVGALSKAKAAKQAAPKETIN
                     NLANLAKAEQQILFRIAQRDTQLDAWKTGFNNRVRKGAGLLDASNIPITINGKTIKPV
                     QAISLKGAPVYSGVSEQEIFALYRQMTGQNPNFRVLPDGRLANGIISTGEWAGTKIAL
                     RNFSKTENSTQARWTLDLQNPPSFIKGTKLELKFQ"
     gene            607..981
                     /locus_tag="ACFM8S_002021"
     CDS             607..981
                     /locus_tag="ACFM8S_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HFC6315028.1"
                     /translation="MDEKQKIKILDFQIDLSSIFNSYKNQMGINIQDKNLKNNFCSFM
                     EELLNDGSIRLHDYTDGIGIPLTGTSKEQVQKLKDIWPTLEDAQAIWPEDPWYYLEWL
                     WWDIACPIDLADLPNIDIYEQA"
BASE COUNT          389 a          218 c          258 g          308 t
ORIGIN      
        1 acgcaggctg ctgaagcggg cgcgggaatc gccacgggtg cggtaacggt aggcaacgct
       61 tgggaagctc cagtaggagc gttgtcgaaa gcgaaggcgg ctaagcaagc tgctcctaaa
      121 gaaacaataa acaatttggc aaatttagcc aaagcagaac agcagatttt attccgtatt
      181 gcccaacgcg atacgcaact ggatgcatgg aagacgggat ttaacaatag agtaaggaaa
      241 ggagcaggct tgcttgatgc aagtaatatt ccgataacca ttaacggaaa aaccatcaaa
      301 cctgtacaag ccataagctt aaagggagca cccgtttaca gcggcgtaag cgaacaggag
      361 atttttgcgc tttatcggca gatgactggc cagaatccga attttagagt tttgcctgac
      421 ggaagattag caaatggcat tatcagtact ggagaatggg caggaacaaa aattgcatta
      481 agaaattttt caaaaacaga gaattcaact caagcacgat ggacattaga tttgcagaat
      541 cctccatcat ttattaaagg tactaaattg gagcttaaat tccaataatt tacaaaggat
      601 tttaccgtgg atgagaaaca aaaaattaag attcttgatt ttcaaatcga tttatcctca
      661 atttttaact cttataaaaa tcaaatgggt attaatattc aagataaaaa cttaaaaaac
      721 aatttctgtt cttttatgga agaactgtta aatgacggtt caatccgttt acatgattat
      781 accgacggta tcggaattcc tctaactgga acttcaaaag aacaagtgca gaaattgaaa
      841 gacatatggc ctactttgga agatgcccaa gcaatatggc ctgaagaccc ttggtattac
      901 ttagaatggc tttggtggga tattgcgtgt ccaatagatt tggccgattt gccgaatatt
      961 gatatttatg agcaagcgta ggtatggtta gccgccttta gcggcgtaac cgtacgcata
     1021 tcagcaaact ttataaaata acaaggccgt ctgaaatctg tttttcaact ttttcagacg
     1081 gccttgcaac ttggcatttc attcgtacgg ttacgccgct aaaggcggct aaccgtacct
     1141 acgagctctg ataaaaatga tttatggaag caa
//
LOCUS       DAXHSF010000245         1123 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.245, whole genome shotgun sequence.
ACCESSION   DAXHSF010000245 DAXHSF010000000
VERSION     DAXHSF010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.245"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(230..1084)
                     /locus_tag="ACFM8S_002022"
     CDS             complement(230..1084)
                     /locus_tag="ACFM8S_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFC6315029.1"
                     /translation="MFYQILALIIWSSSFIAAKYVYGGIDPALMVGVRLLIAALPALP
                     ACRRHVGKIPREEWKPLLIVSFVNYVLTLLLQFVGLKYTSAASASVIVGLEPLLMVFV
                     GHFFFNDKARAYHWICGAAAFAGVALLMAGGAEEGGEVGWFGCLLVLLAGAGFCAAMR
                     PTQRLIARIGAPAFTSVSIAAASLMCLPFSLALAQSYTVDWSVGMVLSLLYLGVGCGW
                     YAYWLWNKGMSRVPANVSGLLISLEPVVGVLLAVLILGEHLSPVSALGVFVVIAATLV
                     AGRLSHQK"
BASE COUNT          309 a          340 c          294 g          180 t
ORIGIN      
        1 cccgtcattc ccgcgcaggc gggaatccgg acatccaacg ctgcagcaat ttgtcggaaa
       61 taaccgaaac tcaaaaaacc ggatgcccgc ctgcgcggga atgacgatgc aaagatggtc
      121 gttatttcgg ataaatatcc gtcttttcgt tccggatacc cgctttcgca ggaatggaga
      181 tggaaagatt tcagcctatt cggcaatcat caaataccgc ttcccaactt tatttttgat
      241 gcgacagccg gccggcaacc aaggtggcgg cgatgacgac aaacacgccc aaggcggaca
      301 cgggcgacag gtgttcgccc aaaatcaaaa ccgccagcag cacgccgacg acgggttcga
      361 gcgaaatcaa cagtcccgac acgttggcag gaacacggct catccccttg ttccacagcc
      421 aataggcgta ccagccgcac cccacgccca aatacagcag cgacaatacc attccgacgc
      481 tccagtccac ggtataactt tgcgccaaag caagcgaaaa cggcaggcac atcaacgatg
      541 cggcggcaat ggaaacagat gtgaatgccg gtgcgccgat gcgtgcaatc agcctttgcg
      601 tcggacgcat agcggcacaa aagcccgcgc ccgccaacaa caccagcagg cagccgaacc
      661 agccgacttc gccgccctct tccgcaccgc ccgccatcag cagcgcgaca ccggcaaatg
      721 ccgccgcgcc gcatatccag tggtaggcac gcgctttgtc gttgaagaaa aagtgtccga
      781 caaacaccat cagcagcggc tcgagtccga caatgaccga tgcgctggcg gcggaagtgt
      841 atttcaaccc gacaaactga agcagcaggg tcagcacata gttgacgaac gacacaatca
      901 gcaacggctt ccattcctca cgcggaatct tgccgacatg acggcggcag gcgggcagtg
      961 caggcagcgc agcaatcagc aggcgcacgc cgaccatcaa tgcgggatcg atgccgccat
     1021 agacatattt ggcggcaata aacgagctgc tccagataat cagggcaagg atttggtaaa
     1081 acatagtgag tgataaagtc agatggtcat acaaaagtat agc
//
LOCUS       DAXHSF010000246         1100 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.246, whole genome shotgun sequence.
ACCESSION   DAXHSF010000246 DAXHSF010000000
VERSION     DAXHSF010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.246"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            1..878
                     /locus_tag="ACFM8S_002023"
                     /pseudo
     CDS             1..878
                     /locus_tag="ACFM8S_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251720.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
BASE COUNT          295 a          323 c          305 g          177 t
ORIGIN      
        1 tgacacccgc agcagcagct gtcgtcgtta tcgtcgtaac cgtattgacc tacggtgcac
       61 tgtccgcccc ggcagccgcc ggaacggcgg gcgcggcagg cgcaggagcg ggaggagccg
      121 cagcaggaac ggcagccgga actggagtag cagcaggaac ggcagccaca accggagtag
      181 cagcaggcac atcagctgca gctatcacca cagccgcagg caaagccgca ctggccagtc
      241 tcgccagcca agccgcagtt tccctcatca acaacaaagg agacataaac cataccctga
      301 aagaactggg caaaagcagc accgtcagac aggccgccac cgccgccgta accgcaggcg
      361 tactgcaggg cataagcggg ctgaacaccc aagcagccga agccgtcagc aaacattttc
      421 acagtcccgc agcaggcaaa ctgaccgcta acctgatcaa cagcaccgct gccgcaagtg
      481 tccataccgc catcaacggc ggcagcctga aagacaactt gggcgatgcc gcactgggtg
      541 cgatagtcag taccgtacac ggagaagtag cgagcaaaat caaatttaat ctcagcgaag
      601 actacattgc ccacaagata gcccatgccg tagcaggctg tgcatcggcg gtagcaaata
      661 aaggcaaatg tcgggacggc gcaatcggcg cggcggtcgg tgaaatcctt ggcgaaaccc
      721 tactggacgg cagagaccct ggcagcttga atgtgaaaga ccgggcaaaa atcattgcta
      781 aggcgaagct ggcagcaggg acggttgcgg cgttgagcaa gggggatgtg aatgctgcgg
      841 cgaatgcggc tgctgtggcg gtagagagta atgcgcttag caaggaaaga atggataaat
      901 tgacaaaatg cctttccggt aaaacttgtt gagacctttg caaaattcct ttccctcccg
      961 acagccgaaa ccccaacaca ggttttcagc tgttttcagc tgttttcgcc ccaaatacct
     1021 cctaattcta cccaaatacc cccttaatcc tccacggaca cccgataatc aggcatccgg
     1081 gctgcttttt aggcggcagc
//
LOCUS       DAXHSF010000247         1073 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.247, whole genome shotgun sequence.
ACCESSION   DAXHSF010000247 DAXHSF010000000
VERSION     DAXHSF010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.247"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            109..543
                     /locus_tag="ACFM8S_002024"
     CDS             109..543
                     /locus_tag="ACFM8S_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HFC6315030.1"
                     /translation="MRRALIAKIKIAQKELGLDDGTYRAVLERVTGKRSCADMDVSEL
                     ESVVADMRSHGFKPKAKGNPHGKPHLRRTSSAAMLDKVEALLTVGGKHWNYAHAMARR
                     MFGKDKVEYLDDTQLHKLVAALQIAENRKTEKAGGDDGVRKS"
     gene            524..952
                     /locus_tag="ACFM8S_002025"
     CDS             524..952
                     /locus_tag="ACFM8S_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFC6315031.1"
                     /translation="MGFEKVEHLLPDTVLDIVDVIGLAATEQLVKAIGGARFKFGKGK
                     VDTERLAILVEAIGEVKTHELLQVYGGEELYVPRCGKALIQLRNHRFYQEFVKLRDID
                     KESGLMAMTKLCPKYGISSRTGYTIINEMSRPAAQQAALF"
BASE COUNT          280 a          194 c          320 g          279 t
ORIGIN      
        1 tttcatgtct gtccgtttcg caaaaaaaat cggcttaata ctatatattg tattttattg
       61 gtataatatg cgttaattag tcaatatatt gtgttttatg ggtttgagat gcgccgggcg
      121 ttgattgcaa aaattaaaat tgcccaaaag gagctgggct tggacgacgg tacctatcgc
      181 gcggtgttgg agcgcgtgac gggcaagcgg tcgtgtgcgg atatggatgt ttccgaactt
      241 gagtctgttg tcgctgatat gcggtcgcac ggatttaagc ctaaagcaaa aggtaaccca
      301 cacggtaaac cacatctgcg tcggacatca tcagcggcaa tgttggacaa agtcgaagcc
      361 ctgctgaccg tcggcggcaa acattggaac tatgcacacg caatggcgcg gcggatgttt
      421 ggtaaggata aggtcgaata tttagacgat acgcagctac ataaactggt tgctgcgttg
      481 cagattgcgg aaaacaggaa aacggaaaaa gcgggtgggg atgatggggt tcgaaaaagt
      541 tgaacattta ttgccggata ccgtgttgga cattgtggat gtcatcggac tggcagcgac
      601 ggaacagctg gtcaaggcga ttggcggggc gcggtttaaa tttggtaagg gcaaggtgga
      661 caccgagcgt ttggcaattt tggtcgaagc catcggcgaa gtgaaaacac atgagctgtt
      721 gcaggtatat ggtggcgagg aattgtatgt cccacggtgc ggcaaggcgt taatacagtt
      781 gagaaaccat aggttttatc aggagtttgt caaattgcgc gatattgata aggagagcgg
      841 gcttatggcg atgacgaagc tatgccctaa atacggcatc tcttcacgaa cgggatatac
      901 gattatcaat gaaatgagcc gacctgcggc acagcaggca gctttatttt aggcagtgat
      961 gtgtgaccag gctttggccg tctgtattca gacggtcttt tttttggttt gcaggggtga
     1021 aacatctacc gttcgggacg atgggttaaa gacggtttaa tggggttttc aaa
//
LOCUS       DAXHSF010000248         1070 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.248, whole genome shotgun sequence.
ACCESSION   DAXHSF010000248 DAXHSF010000000
VERSION     DAXHSF010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.248"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            284..709
                     /locus_tag="ACFM8S_002026"
     CDS             284..709
                     /locus_tag="ACFM8S_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapD"
                     /protein_id="HFC6315032.1"
                     /translation="MSRYLITFDMDTNCLKDNYHGNNYTNAYSDIKTILARHGFENIQ
                     GSVYLGREGISEAHGTIAIQELTARFDWFYSCISNRLESDLNAQFIADGVYQAKQAFL
                     QRVEQLRISLTEAGLSDEQINQVLEKQKFELESPNLKLN"
BASE COUNT          334 a          247 c          218 g          271 t
ORIGIN      
        1 gccaccaaaa cccttgctag ctaagggtta aacagctcac ttgaaatcta cttaagtcta
       61 atctaaacta tccaatatgg atagattttt aaacataggg caagcagcaa aattattgta
      121 gctgaaagca caatcactcg ctggtggtct caaacacgtg ccgactacct cgccgaaaac
      181 actatcagcc gcgataaacc gtgggaaaag ctcgttatca gccgccgcac ttggtactat
      241 cgcgggaaac cgatgctgtc tgaaacgcaa caggagaaaa ataatgagcc gttacctgat
      301 tacctttgat atggatacca actgcctgaa agacaattac cacggaaata actataccaa
      361 tgcctactcc gatattaaaa ccatcttggc tagacatgga tttgagaaca ttcagggcag
      421 tgtttatcta ggccgtgaag gcatcagtga agcacacgga acaatagcca ttcaggaact
      481 gaccgctcgg tttgattggt tttactcctg tatttcaaac cgccttgaaa gtgatttgaa
      541 cgcacaattt atcgctgatg gtgtgtatca agccaaacag gctttccttc aacgtgttga
      601 acaacttcgt atatccctaa cagaagctgg attgtctgat gagcaaatca atcaggttct
      661 ggaaaaacag aaatttgaat tggaaagtcc taacctgaaa ttaaattaac ctcctttact
      721 caccaacatc cgccgcagct ctgtcagttt ttggcgcgct gcggcgattt ctgtgcgttt
      781 tagagcttcg ggtagggtgt gaaacaactc actcgaaatt tacttaagtc taatctaaac
      841 tatccaagca gtaattagta caaaaaaggc aaacttattt taggagctta aaattgcagc
      901 cgcgataaac cgtgggagag tctcggcatt tcccgcgcca cttggtacaa acgtggcaaa
      961 ccgatgccgt ctgaaaccgt acaacaggag acaaaataat gcttgctcaa atcgaactca
     1021 caccctgcca gctaatggtt tacgtaactt aaggttactg gatttacgca
//
LOCUS       DAXHSF010000249         1046 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.249, whole genome shotgun sequence.
ACCESSION   DAXHSF010000249 DAXHSF010000000
VERSION     DAXHSF010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.249"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(299..685)
                     /locus_tag="ACFM8S_002027"
     CDS             complement(299..685)
                     /locus_tag="ACFM8S_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFC6315033.1"
                     /translation="MRKILLDTHALLWWLLDDKELGISARKLIENPRNAIFVSAASIW
                     EISIKQNKGLLKLPEEFFDVLQEEDFEMLPIGLFHAKQAGSLPEIHKDPFDRMLIAQT
                     QAEGFELMTVDEYIPQYGIRVVNASS"
     gene            complement(682..930)
                     /locus_tag="ACFM8S_002028"
     CDS             complement(682..930)
                     /locus_tag="ACFM8S_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFC6315034.1"
                     /translation="MFQANIHQAKTNLSQLIQRAEAGEIVIIAKAGKPCVQLIGIEKP
                     ARNAGRLKKFSHMENTDISRILEDDNETAALFFEESAL"
BASE COUNT          288 a          247 c          181 g          330 t
ORIGIN      
        1 cagccatata tattaagtac ctgctcttta tgcgcttttt taggaaagga agatcaaacg
       61 gttcaatagc gaagtcttgc ccaaatttga tggacaggat atagaataca tgcgtgcata
      121 ccacaccctg ccaaagttgg gagatgcact taaggttgca cttgtggtcg ccaactatgc
      181 ctatacacgc ggatttcccg cgtttatagg ccgcgacgga agtctggctt ttaagcgaag
      241 acaataaccg aaaagaccgc ctcggcggtc tttcttgtgt acacagtttt tatatttgtc
      301 agcttgatgc attgacaact ctaattccat attgcggaat atattcatcg acagtcatca
      361 gttcaaagcc ttccgcttgg gtttgtgcaa tcaacatcct atcgaaaggg tctttgtgta
      421 tctccggaag gcttccagcc tgttttgcat gaaacagacc tataggcaac atttcaaaat
      481 cctcttcttg aagcacatca aaaaactctt ccggtaattt caacaacccc ttgttctgct
      541 tgatggaaat ttcccaaata cttgctgcac tgacaaagat cgcatttctc ggattttcta
      601 tcagtttgcg tgcagatatc cccagttcct tgtcatccaa caaccaccac agcaacgcat
      661 gggtatcaag cagaatcttt ctcacagagc cgactcctca aaaaataaag ctgccgtttc
      721 attgtcatcc tcaagaatac gtgaaatatc cgtattttcc atatgactga attttttcaa
      781 ccttcctgca tttcgtgccg gtttttcaat accgattagt tggacgcaag gcttacctgc
      841 cttcgcaata ataacaattt cccctgcttc tgctctttga atcaattgac tcaaattggt
      901 ttttgcctga tgaatatttg cttgaaacat aacacttctc atgattagct aacttaacta
      961 atatacatca ttaccaagat tttgggaatc tcattacata tatttgatta tatccgccgt
     1021 tttattcaca ccttgctatt tatagt
//
LOCUS       DAXHSF010000250         1016 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.250, whole genome shotgun sequence.
ACCESSION   DAXHSF010000250 DAXHSF010000000
VERSION     DAXHSF010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.250"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(137..769)
                     /gene="mtrR"
                     /locus_tag="ACFM8S_002029"
     CDS             complement(137..769)
                     /gene="mtrR"
                     /locus_tag="ACFM8S_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux system transcriptional
                     repressor MtrR"
                     /protein_id="HFC6315035.1"
                     /translation="MRKTKTEALKTKEHLMLAALETFYRKGIARTSLNEIAQAAGVTR
                     GALYWHFKNKEDLFDALFQRICDDIENCIAQDTEDAEGGSWAVFRHTLLHFFERLQSN
                     DIYYKFHNILFLKCEHTEQNAAVIAIARKHQAIWREKITTVLTEAVENQDLADDLDKE
                     TAVIFIKSTLDGLIWRWFSSCERFDLGKTAPRIIGIMMDNLENHPDLRRK"
BASE COUNT          247 a          217 c          260 g          292 t
ORIGIN      
        1 agattgagca taaaatttta gtaacctatg ttattgcaaa ggtctcaata tgtattttta
       61 gaagaaggct ttgtgtcatt ttgatgccgt ctgaaagggt ttgttcgttt cagacggcat
      121 tgttaccaag gcttgattat ttccggcgca ggtcgggatg gttttccaag ttgtccatca
      181 ttatcccgat gatgcgcggg gcggttttgc ccaaatcgaa acgttcgcag gaagagaacc
      241 accgccaaat cagcccgtcc aaggttgatt tgatgaagat aactgccgtt tccttgtcca
      301 aatcgtcagc caaatcctga ttttccaccg cttcggtcaa aacggtggta attttctcgc
      361 gccagattgc ctgatgcttg cgggcaatgg cgataacggc ggcgttttgc tccgtgtgtt
      421 cgcattttaa aaacaggatg ttgtggaatt tgtagtagat gtcgttgctt tgcagccgct
      481 cgaaaaagtg cagcagcgtg tggcggaata ccgcccaaga ccctccttcg gcatcttcgg
      541 tatcttgcgc gatgcagttt tcgatgtcgt cgcagatacg ttggaacagc gcgtcgaaca
      601 agtcttcctt atttttgaaa tgccaataga gcgcgccgcg cgttacgccg gcggcttggg
      661 cgatttcgtt gagcgaggtg cgcgcaatcc ctttgcggta aaaggtttcc aaggcggcaa
      721 gcatcaggtg ttctttggtt ttcaaggctt cggttttggt ttttctcata atggcgtttt
      781 cgtttcgggt cggtttgatg agggcggatt ataaaaaaga ctttgtaacc atgcaatcgt
      841 gtatgtataa tgaaacccat gaaattgaga ctacatctca actttgaaaa caggcataac
      901 ctgctttgca acccgttcga acattcgggt atcaaagcag gcggttttta tatcctgaaa
      961 tatagtggat taacaaaaat caggacaagg cgacgaagcc gcagacagta cagaca
//
LOCUS       DAXHSF010000251         1015 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.251, whole genome shotgun sequence.
ACCESSION   DAXHSF010000251 DAXHSF010000000
VERSION     DAXHSF010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.251"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            62..>1015
                     /locus_tag="ACFM8S_002030"
     CDS             62..>1015
                     /locus_tag="ACFM8S_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HFC6315036.1"
                     /translation="MLKTPPTLAAELSGKTGVSISAPYANENSRILLSTTDISSENGK
                     IKIQSYGDQYYYARQSELYTFERRSYKTGKWYNRKHITEVKEHKNAKPDAVNLSASQG
                     IDIKSGGNIDAYATAFDAPKGSINIEAGRKLTLYAVEELNYDKLDSQKRRRFLGISYS
                     KAHDTTTQVMKTALPSRVVAESANLQSGWDTKLQGTQFETTLGGAVIRAGVGEQARAD
                     AKIILEGIKTTIHNETVSSSKSTLWQKQAGRGSNIETLQLPSFTGPVAPVLSAPGGYI
                     VDIPKGNLKTEIEKLAKQPEYAYLKQLQVAKNVNWNQVQLAY"
BASE COUNT          322 a          294 c          224 g          175 t
ORIGIN      
        1 cagacggcat ttgttatgca agtaaatatt cagattccct atatactgcc cagacgcgtg
       61 cgtgctgaag acacccccta cgcttgctgc agaactttcg ggtaaaaccg gtgtgagcat
      121 tagcgcaccg tatgccaatg agaacagtcg catcctgctc agcaccacgg atatcagttc
      181 ggaaaacggc aaaatcaaaa ttcaatctta cggtgaccaa tattactatg cgagacagag
      241 cgaactctat acctttgaac gccgcagcta caaaaccggc aaatggtaca accgcaaaca
      301 cattaccgaa gtcaaagaac acaaaaacgc caagcccgac gcagtaaacc tcagcgcatc
      361 ccaaggcatc gacatcaaat ctggtggcaa catcgacgcc tacgccaccg cattcgatgc
      421 ccccaaaggc agcattaaca tcgaagccgg gcggaaattg acactctatg ccgtagaaga
      481 gctcaactac gacaaactag acagccaaaa aaggcgcaga tttctcggca tcagctacag
      541 caaagcacac gacaccacca cccaagtcat gaaaaccgcg ctgccctcaa gggtagttgc
      601 agaatctgcc aatctgcaat caggctggga taccaaactg caaggcacac agtttgaaac
      661 cacactgggt ggcgcggtca tccgtgcagg cgtaggcgag caggcacggg cagatgccaa
      721 gattatcctc gaaggcatca aaaccacgat ccacaacgaa accgtgagca gcagcaaatc
      781 tactctatgg caaaaacagg caggacgcgg cagcaacatc gaaaccctgc aactgccaag
      841 tttcaccggc cccgttgcgc ccgtactgtc cgcacccggc ggctacattg tcgatattcc
      901 caaaggcaat ctgaaaaccg aaatcgaaaa acttgccaaa cagcccgagt atgcttattt
      961 aaaacagctt caagtggcga aaaacgtcaa ctggaatcag gtgcagcttg cttac
//
LOCUS       DAXHSF010000252          941 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.252, whole genome shotgun sequence.
ACCESSION   DAXHSF010000252 DAXHSF010000000
VERSION     DAXHSF010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.252"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(<1..216)
                     /locus_tag="ACFM8S_002031"
     CDS             complement(<1..216)
                     /locus_tag="ACFM8S_002031"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6315037.1"
                     /translation="MPSVELLLVIVGLPRGTFYYQLVVQSAEDKYADLKRHIHDIYQK
                     QLKDNGLVQSMSRKGNCLDNAAMESFFG"
     gene            complement(413..526)
                     /locus_tag="ACFM8S_002032"
     CDS             complement(413..526)
                     /locus_tag="ACFM8S_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC6315038.1"
                     /translation="MSQKAAADQLNLPDCSILLQWLRLYHLNGINGLKPKP"
BASE COUNT          222 a          229 c          205 g          285 t
ORIGIN      
        1 tccgaagaaa ctttccattg ccgcattgtc caagcagttt cccttgcggg acatactctg
       61 aaccagaccg ttgtctttca actgcttttg ataaatatca tggatatgcc gtttcaaatc
      121 ggcatatttg tcttctgccg attggacaac caattggtaa tagaaggtgc cgcgtggcag
      181 tccgacaatc accaacagca gttcaacgga tggcattgcc ttaaccctgc gacgagttgc
      241 gttctttcta ccgcacttct ttcccataga ttaaggcatc gagctttttt agggcagcca
      301 tttccgcttt aaggcaagcc aattccgcaa gcagttcttc cttggttttc agatagtcgg
      361 ctttttcgtt tccggcggat gctgtttttt cacgggcttt cttcctttgg gtttagggtt
      421 tgggctttaa accgttaata ccattcaaat ggtagaggcg caaccattgc agcaagatgg
      481 agcagtcggg caaattcagt tggtctgcgg cagctttttg ggacattccc taccccgtca
      541 ccaggcggat tgcctcaagt ttgtattcga ccgaatattt tgtcgtatgc tttctacgtt
      601 tgatgccact ctctccgtgt aatctgtatt ttgtcaccca tctgcgtacc aatgaatcgg
      661 aaatagaaag atggtctgct gttccctgcc aaatagtatt gaacgacggc aagtcggaat
      721 tcatctgaat attttgccat aaaaaactgc accccctaaa gtcggtaagg tgtccaactt
      781 ttggggtgca gttcagaagc ggtctttttt tgcctgccgg ttttgaatca tcctccgtgt
      841 atattccctt gacgaaaaaa atgatgatat tacggatacc aaaactaagg tcgtatccgc
      901 ccccctactc tccctaagca aagagatgaa acagcgtatc g
//
LOCUS       DAXHSF010000253          906 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.253, whole genome shotgun sequence.
ACCESSION   DAXHSF010000253 DAXHSF010000000
VERSION     DAXHSF010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.253"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(55..834)
                     /locus_tag="ACFM8S_002033"
     CDS             complement(55..834)
                     /locus_tag="ACFM8S_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFC6315039.1"
                     /translation="MKLVRPQKISDQVLSVLEERIAEGVYAEGGKIPPERVLAEEFGV
                     SRPSVRSALNVLVARQVLEARQGDGYYVSVKPQQDFLQSWQELLGKHSNWEQDVFDFS
                     CHIEGCMAALAAERRTDADLKRIGFWLEKFEEACESGNLEHQSEADVSFHQTIADAAH
                     NLLFSHLSGGLLKMLYRQTRSSLIYLNQEEDPRPKLMAQHRVLYEAISNRRPGEASEA
                     AKAHLNYVASSIMKDREYQSRNRHADTLAQNDLKRVQDWEV"
BASE COUNT          222 a          291 c          179 g          214 t
ORIGIN      
        1 aatttagttc actataccga tacatccgaa tatgccagac cgtctaaacg gtattcatac
       61 ctcccaatcc tgcacgcgct tcaaatcgtt ttgcgccaaa gtatccgcgt ggcggttacg
      121 gctctgatat tccctgtctt tcatgatgct gcttgccaca taattcaaat gtgcctttgc
      181 cgcctccgaa gcctcgcccg gccgacggtt cgatatcgcc tcatacagca cgcggtgctg
      241 cgccatcagt ttcggacgcg gatcttcttc ctgattcaga taaataaggc tgctgcgcgt
      301 ctgccggtac agcattttca acaaaccgcc cgacaaatgg ctgaacaaca aattgtgcgc
      361 cgcatccgca atcgtctgat ggaagctgac atcggcttcg ctctgatgtt ccagattgcc
      421 gctttcgcac gcctcctcaa acttttcaag ccaaaaccca atccgcttca aatcggcatc
      481 cgtccggcgt tctgccgcca atgccgccat acagccctcg atgtggcagc tgaaatcaaa
      541 aacatcctgt tcccaattgg aatgcttgcc caaaagctcc tgccaacttt gcaaaaaatc
      601 ctgctgcggc ttgaccgaaa cataataacc gtctccctgc ctcgcttcca aaacctgacg
      661 ggcaaccaaa acattcaatg ccgaccggac cgacgggcgc gaaacgccga actcttccgc
      721 caaaacgcgt tcgggcggaa tcttgccccc ttccgcgtaa accccttccg caatgcgctc
      781 ctccaatacc gacaatacct gatcgctgat tttctgaggc cttaccagtt tcatcactcc
      841 tcctttataa agattccctg cagaaccctt ccgaaatata gtggattaac aaaaatcagg
      901 acaagg
//
LOCUS       DAXHSF010000254          894 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.254, whole genome shotgun sequence.
ACCESSION   DAXHSF010000254 DAXHSF010000000
VERSION     DAXHSF010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.254"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(70..678)
                     /locus_tag="ACFM8S_002034"
                     /pseudo
     CDS             complement(70..678)
                     /locus_tag="ACFM8S_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581764.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          238 a          185 c          186 g          285 t
ORIGIN      
        1 gttggaatat agggttaaaa tagggctctt ttgggcttat tcaaattagg gacacgccct
       61 aaattttggt ccaagctgtt ctatatacgc ctcctcagtc cgttttttat tttcttccca
      121 agcttcgggt cttgttttgt ataaatcctg taattgcttc ttaaccgcat cctttaaatt
      181 atccccgtaa gtatctgtaa tatatttctt aacaacgggc atattatcga cggcgagtat
      241 gccgctattg tagtttttac cttggatatt gatttcgtca tacccatatt cggatgaatt
      301 ttgatcggtt ttaccgctgg aaaaataatg ggaagtgccg ggtttcggct tgtctgaaaa
      361 tgaaaaatcg gcatcgtcgc cgattttggt agaacgagta tttgaaccaa gctgagctct
      421 gaacataccg aagcaatcaa aagatatgct gcacgggccg cgccaaacgt gggaatttct
      481 gtcttccgcc gcttctaaaa taaatttgtt tttaatctcg gcttctttga cgggatcgta
      541 cgcatccgaa cctcaaacat atggcgtgca aaacagcgat aaaacagata atgttaatag
      601 attaacccga ccgcttgctt gcttgcttgc ttgcttgctt gcttgcttgc ttgcttgctt
      661 gcttgctttc agtgtcatga gaaattttcc tttgtcaagt gttaaaatta taatgattat
      721 atactacatt atactacatc gcaatgaaaa atcaggaaaa gcaaaaaacc ctccgccgtc
      781 attcccgcga aagcgggaat ctagaatttt aatgccgcaa gaatttatca aggatggtcg
      841 gggtttaaag gtctggattc ccgcctgcgc gggaatgacg gtttagaaat cacc
//
LOCUS       DAXHSF010000255          835 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.255, whole genome shotgun sequence.
ACCESSION   DAXHSF010000255 DAXHSF010000000
VERSION     DAXHSF010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.255"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     repeat_region   375..806
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=441..476
                     /rpt_unit_seq="gttgtagctcccattctcatttcgcagtgctacaat"
BASE COUNT          213 a          190 c          164 g          268 t
ORIGIN      
        1 tttgcaagat tttagtttaa tcatcaaaca gggaacagca aaaatccccg ctgcaacggg
       61 gatttttgtt cggctctcgc ctgtgtgatt aacttcacta ggagaagcta aaatgcttaa
      121 attccttatg cgtgggaaaa agttgctgct gtccgttgaa gtgaaccaca agttcatttt
      181 agcggttgct ttgctgttaa gtcaatgcag tagctaaccc gaccacaatc aaaaaaacgc
      241 aagttttccg cctactctgt gagtgggcgg ttttttctta tttcaaatct gtgacacaat
      301 atctgtcgcc tttcttgcca taattgcact gtgtctcaat gactttaacg cacgttcgct
      361 tatcgcaacg gctggttgta gcgcccattc tcatttcgca gtgctacaat ggacggcggt
      421 tctaaaatat gaaaactcaa gttgtagctc ccattctcat ttcgcagtgc tacaataaac
      481 tcaacgcatc taacatagtt cacactgttg tagctcccat tctcatttcg cagtgctaca
      541 atggtcagat tataataacg ttgagcgcgt gtgttgtagc tcccattctc atttcgcagt
      601 gctacaatta tcatgatgag catatttatg aagaggtagt tgtagctccc attctcattt
      661 cgcagtgcta caattaattt gcgtttcgtg tcgctcataa acccgttgta gctcccattc
      721 tcatttcgca gtgctacaat atatcgtact cttttacgtt attaacaaat gttgtagctc
      781 ccattctcat ttcgcagtgc tacaatctga aaggtaagga acgggcggct tgtga
//
LOCUS       DAXHSF010000256          829 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.256, whole genome shotgun sequence.
ACCESSION   DAXHSF010000256 DAXHSF010000000
VERSION     DAXHSF010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.256"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
BASE COUNT          199 a          201 c          221 g          208 t
ORIGIN      
        1 ggcgaaccaa cgctgtactg gtttagattt aattcactat acctgcgtaa tggtatcggg
       61 tactcatcat caacctgcct tgcattcggt tgtgttgtcc ggcaactgtc cggttgtgtc
      121 gatgggttgc cctgccgctc cctgtgctgc gcaacggttg tgtgccttgg gtgggcgcgg
      181 gtggcggccg ggcggtttgt tgctatgata cgggagttcc gatatacaag ggtgggcttc
      241 agcccaccaa tcaacgaccg aaaaagaaaa aacgttgggc tgaagcccac tctacgcaat
      301 tacgcaactg ttatcgtggg gatgacggga ttttaggttt ttgtttttct gtttttgcgg
      361 gaaggatggg ttttaggttg cggtcattta tcggaaaagc agaaatcgct ccgccgtcat
      421 tcccgcgaaa gtgggaatct gaacacgtcc gtagggaaac ctatatcccg tcattcccac
      481 gaaagtggga atctagaacc tctgaatttt cagacaacct ttgaatattg ccgccgtccc
      541 gcgttctaga ttcccgcctg cgcgggaatg acgattcatc agtttcccga aacgcccgaa
      601 aaaccgaaac cgagcaaacc ggattcccgc ctgcgcggga atgacgattt ataagtttcc
      661 cgaaacaccc aaaaaaaccg aaatccgaca ggcaggattt ttgctttcgt ggggatgacg
      721 ggattttaag ttacggtcat ttatcggaaa agcagaaact gctccgccgt tattccagcg
      781 aaggcgggaa tctgaacacg tccgcacgga aacctatagt ggattaaca
//
LOCUS       DAXHSF010000257          817 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.257, whole genome shotgun sequence.
ACCESSION   DAXHSF010000257 DAXHSF010000000
VERSION     DAXHSF010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.257"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(<1..409)
                     /locus_tag="ACFM8S_002035"
     CDS             complement(<1..409)
                     /locus_tag="ACFM8S_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6315040.1"
                     /translation="MKNNIFLNLNKKSINNNYFVISIFFETIYQFETKDTLLECFKNI
                     TTTGHFGVIGAQYEKIDATRWIGDYEEVNGFEYIDKAPSIYFSVGDDFNPEELIIPIN
                     LAYHYFNIAISDFLIAHPEYQKKCKEIQKTYSQT"
     gene            complement(425..>817)
                     /locus_tag="ACFM8S_002036"
     CDS             complement(425..>817)
                     /locus_tag="ACFM8S_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdiA family toxin C-terminal domain-containing
                     protein"
                     /protein_id="HFC6315041.1"
                     /translation="HNRTNFMAELNSRGGRVKSETQTDIEGITRIKYGIPTLDRTGKP
                     DGGFKEISSIKTVYDPKKFSDDKILQMAQNAASQGYSTASKIAQNERTKSISERKNVI
                     QFSETFDGIKFRSYFDVNKGRITNIHPE"
BASE COUNT          254 a          116 c          114 g          333 t
ORIGIN      
        1 ttgtttgaga atatgttttt tgtatttctt tacacttttt ttgatattca gggtgagcta
       61 ttaagaaatc agatattgca atattaaagt aatgatatgc taaattaata ggtataatta
      121 attcttcagg attgaaatca tctccaactg aaaaataaat agaaggagct ttatcaatat
      181 actcaaatcc atttacctct tcataatctc caatccatct agtagcatct attttttcat
      241 attgagcacc tattactcca aaatgtccgg tagttgtaat atttttaaaa cactctaaaa
      301 gcgtatcttt agtttcaaat tggtaaattg tttcaaaaaa aatcgaaata acaaaatagt
      361 tgttatttat agatttttta tttaagttta gaaaaatatt atttttcata atttttcctt
      421 taaattattc tgggtgaatg tttgtaattc ttcctttatt tacatcaaaa tatgatctaa
      481 atttgattcc gtcaaaggtt tctgaaaatt gaatgacatt ttttctttcc gatattgatt
      541 tagttctttc attttgagca attttagagg ctgttgaata tccttgtgaa gcagcatttt
      601 gagccatttg aagtatttta tcatcagaaa attttttagg atcataaaca gtttttatac
      661 ttgaaatttc cttaaatcca ccatcaggtt tacctgtcct gtctagtgta ggaatcccat
      721 atttaattcg ggtaatgcct tcaatatcag tttgggtttc agattttacg cgtcctcctc
      781 gtgaatttag ttctgccata aaattggtgc ggttatg
//
LOCUS       DAXHSF010000258          809 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.258, whole genome shotgun sequence.
ACCESSION   DAXHSF010000258 DAXHSF010000000
VERSION     DAXHSF010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.258"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..>809
                     /locus_tag="ACFM8S_002037"
     CDS             <1..>809
                     /locus_tag="ACFM8S_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HFC6315042.1"
                     /translation="QMGADIAQLNSKVRTELFSSTGNQTIANLGGRLATNLSNAGISA
                     GINTAVNGGSLKDNLGNAALGALVNSFQGEAASKIKTTFSDDYVAKQFAHALAGCVSG
                     LVQGKCKDGAIGAAVGEIVAESILGGRNPATLSDAEKHKVISYSKIIAGSVAALNGGD
                     VNTAANAAEVAVVNNALNFDSTPTNAKKHQPQKPDKTALEKIIQSIMPAHAAGAMTNP
                     QDKDAAIWISNIRNGITGPIVITSYGVYAAGWTAPLIGTAGKAAISTCMANP"
BASE COUNT          237 a          189 c          216 g          167 t
ORIGIN      
        1 cagatgggag cagatattgc ccaattgaac agcaaggtaa gaaccgaact gttcagcagt
       61 acgggcaatc aaaccattgc caaccttgga ggcagattgg ccaccaacct cagcaatgca
      121 ggtatctcag ctggtatcaa taccgccgtt aacggcggca gcttgaaaga caacttaggc
      181 aatgccgcat tgggagcatt ggttaatagc ttccaagggg aagccgccag caaaatcaaa
      241 acaaccttca gcgacgatta tgttgccaaa cagttcgccc acgctttggc gggttgtgtt
      301 agcggactgg tacagggaaa atgtaaagat ggggcaattg gcgcagcagt tggggaaatc
      361 gtagctgaat ccatacttgg cggcagaaac cctgctacac tcagcgatgc agaaaagcat
      421 aaagttatca gttactcgaa gattattgcg ggcagcgtgg cggcactcaa cggcggcgat
      481 gtgaatactg cggcgaatgc ggctgaggta gctgtggtga ataatgcttt gaattttgac
      541 agcaccccta ccaatgcgaa aaagcatcaa ccgcagaagc ccgacaaaac cgcactggaa
      601 aaaatcatcc aaagtattat gcctgcacat gcggcaggtg cgatgactaa tccgcaggat
      661 aaggatgctg ccatttggat aagcaatatc cgtaatggca tcacaggccc gattgtgatt
      721 accagctatg gagtttatgc agcaggttgg acagctccgc tgatcggtac agctggtaaa
      781 gcagctatca gcacctgtat ggctaatcc
//
LOCUS       DAXHSF010000259          806 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.259, whole genome shotgun sequence.
ACCESSION   DAXHSF010000259 DAXHSF010000000
VERSION     DAXHSF010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.259"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..205
                     /locus_tag="ACFM8S_002038"
     CDS             <1..205
                     /locus_tag="ACFM8S_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6315043.1"
                     /translation="IVATPLDSSQGNALGSSTTEYDFGGSANFEQFRNAQFPIEANLN
                     VEIVTTGKTQKLKVIGFQLVKKG"
     gene            210..407
                     /locus_tag="ACFM8S_002039"
     CDS             210..407
                     /locus_tag="ACFM8S_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6315044.1"
                     /translation="MQKVYVVQSVSTGDFLYLSPETGDIGHTKLITNADYFYDFEEAI
                     NAGLEEIGNQYEFVVFGFLKD"
     gene            481..699
                     /locus_tag="ACFM8S_002040"
     CDS             481..699
                     /locus_tag="ACFM8S_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFC6315045.1"
                     /translation="MKFINTCRKYGAKLAVVTAAPLALAAHANATLPDTAKNALEAAK
                     ADGMEAGWIVVGVFAALFVFSIVKRVMK"
BASE COUNT          238 a          187 c          180 g          201 t
ORIGIN      
        1 gattgtcgcc acacccttag acagctccca aggcaacgca ttgggcagct ctactactga
       61 atacgatttc ggcggctctg ccaatttcga gcagttccga aacgcccaat ttccgatcga
      121 agcaaacctg aacgtagaaa tcgttactac gggcaaaacc caaaaactga aagtcatcgg
      181 ttttcaactc gtgaagaaag gctgattgaa tgcagaaagt ctatgttgtc cagtccgtat
      241 caacagggga ctttctgtat ctctctcctg aaacgggcga catcggacat accaaattaa
      301 tcaccaatgc cgattatttc tacgacttcg aagaagcgat taacgcaggt ttggaagaaa
      361 tcggcaacca atacgaattt gtcgtattcg gatttttgaa agactgattt tcggatgttc
      421 ggcggtcgtc tgaaaaacgc tccatccatt accgccaaac actttttgaa ggaaaatatc
      481 atgaaattta ttaacacctg ccgtaaatac ggcgcaaaac tggctgttgt aacagctgct
      541 cccctggctt tggccgcaca tgcaaatgca acgttgcccg atacagcaaa aaacgctttg
      601 gaagccgcaa aagccgacgg tatggaagcc ggttggattg tcgttggcgt tttcgccgcg
      661 ctttttgtct tttccatcgt taagagagtg atgaagtaag acggcatgta ctaccaagtc
      721 ggaaataaat gtcttgagaa gcaccaggct gaaaaccttt atttcagctt ggtagtacca
      781 agaatcaaag aaaacggaca gattgt
//
LOCUS       DAXHSF010000260          805 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.260, whole genome shotgun sequence.
ACCESSION   DAXHSF010000260 DAXHSF010000000
VERSION     DAXHSF010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.260"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(479..>805)
                     /locus_tag="ACFM8S_002041"
     CDS             complement(479..>805)
                     /locus_tag="ACFM8S_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFC6315046.1"
                     /translation="GDNTSAGVATSTDIKGKYVKEVEVNNGVITATMLSSGVNKEIQG
                     RKLSLWAKRQDGSVKWFCGQPVTRAGTATADANGTDKKIDTKLEKPFYPISFRTDRPR
                     FPPARE"
BASE COUNT          183 a          167 c          204 g          251 t
ORIGIN      
        1 cgttctggat tcccgctttc gcgggaatga cgggattaaa gtttcaaaat ttattctaaa
       61 taactgaaat tcaacgaact agattcccac tttcgtggga atgacgaatt ttaggttgct
      121 gtttttggtt ttctgtcctt gtgggaatga tgaaaattta agttttagga atttatcggg
      181 agcaacagaa accgctccgc cgtcattccc gcgcaggcgg gaatccagcc tcgtcggtac
      241 ggaaacttat cgggaaaacg gtttcttgag attttgcgtt ctggaccccc actttcgcgg
      301 gaatgacggg attaaagttt caaaatttat tctaaataac tgaaactcaa cgaactagat
      361 tcccactttc gtgggaatga cgaattttag gtttctgttt ttggttttct gtttttgagg
      421 gaatgatgaa attttaagtt ttaggaattt atcagaaaaa acagaaaccg ctccgccgtc
      481 attcccgcgc aggcgggaat ctaggtctgt cggtacggaa acttatcggg taaaaaggtt
      541 tctctagttt ggtgtcgatt ttcttgtcgg tgccgttggc gtcggcggtg gcggtgccgg
      601 cgcgcgtaac cggctgtccg cagaaccatt ttaccgaacc gtcttgacgc ttggcccaca
      661 gggagagttt tctgccttgg atttctttgt ttacgccgct tgaaagcatt gtggcggtaa
      721 tgacgccgtt gttgacttca acttccttaa catattttcc tttaatgtcg gtggaggttg
      781 ccacgccggc agaagtgttg tcgcc
//
LOCUS       DAXHSF010000261          797 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.261, whole genome shotgun sequence.
ACCESSION   DAXHSF010000261 DAXHSF010000000
VERSION     DAXHSF010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.261"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            408..614
                     /locus_tag="ACFM8S_002042"
                     /pseudo
     CDS             408..614
                     /locus_tag="ACFM8S_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311098.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
     gene            629..797
                     /locus_tag="ACFM8S_002043"
                     /pseudo
     CDS             629..797
                     /locus_tag="ACFM8S_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tonB dependent receptor family protein"
BASE COUNT          217 a          219 c          170 g          191 t
ORIGIN      
        1 tcaaacatca aattggactg accaatcagg gcggattcta atgacacgcc gttcccgccg
       61 tcaacggcat ttacctcgca ccgcccccga aacacaagaa aaaactacac caaactacaa
      121 tttttgttta tgcaaatatt tgttttgaca ggatttaaac gaaagctccg attcaaatct
      181 gccgaaccac ccaaaaatat atagtggatt aacaaaaatc aggacaaggc gacgaagccg
      241 cagacagtac agatagtacg gtaaggcgag gcaacgccgt actggtttaa atttaatcca
      301 ttctattgac ctgaatatta aagtttcgca aagtaatgca acgttgcttt aattggtttg
      361 accactattg ccgacgatta gaaaaatatt ttcggagatg ttcaattatg gaaacttggg
      421 ttcaaaacta cacggcaatc ggcggcagcc tgtatctgac tgccgccgcc gcactcttac
      481 ccatcgtctt tttctttgcc gcgctgaccg tcctgaagct gaaaggctat caggcggggc
      541 tttatacgct gctgattgca cttgccgttg ccgtattcgg cttcgggatg ccggcgggta
      601 tggcggtttc ttccctgccg ccgcagccgc actgacccaa cgcccctacg ccacgctgga
      661 tttgaccgcg cattacaaaa tcggcaaatc cacccgcatt ggtttggact ttgaaaacgt
      721 gttcaacaaa cgctaccgcc ctatgcccga cattcacgtt tacggcacgc cgcgcagcct
      781 gaccgcaacc atcaaac
//
LOCUS       DAXHSF010000262          768 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.262, whole genome shotgun sequence.
ACCESSION   DAXHSF010000262 DAXHSF010000000
VERSION     DAXHSF010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.262"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..>768
                     /locus_tag="ACFM8S_002044"
     CDS             <1..>768
                     /locus_tag="ACFM8S_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFC6315047.1"
                     /translation="RSKPHVNVGTIGHVDHGKTTLTAALTTILAKKFGGAAKAYDQID
                     NAPEEKARGITINTSHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAA
                     DGPMPQTREHILLARQVGVPYIIVFMNKCDMVDDAELLELVEMEIRDLLSSYDFPGDD
                     CPIVQGSALKALEGDAAYEEKIFELAAALDSYIPTPERAVDKPFLLPIEDVFSISGRG
                     TVVTGRVERGIIHVGDEIEIVGLKETQKTTCTGVEMFR"
BASE COUNT          204 a          224 c          189 g          151 t
ORIGIN      
        1 acgtagcaaa ccgcacgtaa acgttggcac catcggtcac gttgaccatg gtaaaaccac
       61 cctgactgcc gctttgacta ctattttggc taaaaaattc ggcggtgctg caaaagctta
      121 cgaccaaatc gacaacgcac ccgaagaaaa agcacgcggt attaccatta acacctcgca
      181 cgtggaatac gaaaccgaaa cccgccacta cgcacacgta gactgcccgg ggcacgccga
      241 ctacgttaaa aacatgatta ccggcgccgc acaaatggac ggtgcaatcc tggtatgttc
      301 cgcagccgac ggccctatgc cgcaaacccg cgaacacatc ctgctggccc gtcaagtagg
      361 cgtaccttac atcatcgtgt tcatgaacaa atgcgacatg gtcgacgatg ccgagctgtt
      421 ggaactggtt gaaatggaaa tccgcgacct gctgtccagc tacgacttcc ccggcgacga
      481 ctgcccgatt gtacaaggtt ctgcactgaa agccttggaa ggcgatgccg cttatgaaga
      541 gaaaatcttc gaactggctg ccgcattgga cagctacatc ccgactcccg agcgtgccgt
      601 ggacaaaccg ttcctgctgc ctatcgaaga cgtgttctcc atttccggcc gaggtacagt
      661 agtaaccggc cgtgtagagc gcggtatcat ccacgttggt gacgagatcg aaatcgtcgg
      721 tctgaaagaa acccaaaaaa ccacctgtac cggtgttgaa atgttccg
//
LOCUS       DAXHSF010000263          761 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.263, whole genome shotgun sequence.
ACCESSION   DAXHSF010000263 DAXHSF010000000
VERSION     DAXHSF010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.263"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(38..604)
                     /locus_tag="ACFM8S_002045"
     CDS             complement(38..604)
                     /locus_tag="ACFM8S_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HFC6315048.1"
                     /translation="MGFYALLLIALGMSMDAFAVALAKGAAVRMPPRKIAATALVFGT
                     VEALTPLAGWVGGFYAKPFISEWDHWAAFVLLGGLGLKMMREGLSGKAEDVRESKRES
                     WWLTVLTAFGTSIDSMIVGVGLAFMEVNIAFAAAVIGMATTVMVTVGLTAGRALGVLF
                     GRCAEFAGGLVLIAIGTWTLLSHLGLIQ"
BASE COUNT          214 a          258 c          155 g          134 t
ORIGIN      
        1 cgtactggtt tttgttaatc cactatattt tccgacatca ttgaatcaaa cccaaatgcg
       61 acaagagcgt ccatgtgccg atggcaatca acaccaaacc tccggcaaat tccgcacacc
      121 tgccgaacaa tacgcccaaa gcccttcccg ccgtcagccc gaccgtaacc atcaccgtcg
      181 tcgccatgcc gatgacggcg gcggcaaagg cgatgtttac ctccataaac gccaagccca
      241 ccccgactat catggaatca atactggttc caaaagcagt cagaaccgtc aaccaccagc
      301 tttcccgttt gctttcgcgt acatcttccg ccttgccgga cagcccttcg cgcatcattt
      361 tcagacccag cccgcccagc aggacgaaag ccgcccaatg gtcccattcg ctgataaacg
      421 gtttggcata aaaaccgcct acccagcctg ccagcggcgt gagtgcctca accgtgccga
      481 acaccaaagc cgttgctgca attttgcgcg ggggcatcct gaccgccgca ccctttgcca
      541 gcgcgacggc aaacgcatcc atcgacatcc ccagcgcaat caagagcaaa gcataaaaac
      601 ccataccgca cccgtcctta aaaagggcgg attatagcaa aagcaaaaaa atgcaaaaat
      661 gccgcacgaa aacccgcatc ccgtcattcc cgcaaaaaca aaaaatcaaa aacagaaatc
      721 ccgtcattcc cgcgcaggcg ggaatccaga gttgtcggtg c
//
LOCUS       DAXHSF010000264          761 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.264, whole genome shotgun sequence.
ACCESSION   DAXHSF010000264 DAXHSF010000000
VERSION     DAXHSF010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.264"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(10..761)
                     /locus_tag="ACFM8S_002046"
     CDS             complement(join(10..542,542..761))
                     /locus_tag="ACFM8S_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242698.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS1301 family transposase"
                     /protein_id="HFC6315049.1"
                     /translation="MARTAITDNIWEQLQTTMKAHGCHQWKNDRTVMEAILWKLRTGA
                     PWRDIPIELGSWKTAYNRFNRWSKKGLWQKFFFDLRKEIDKEWVFIDGSYVRCHQHAS
                     GARRGFDRAIGQSRGGNTTKIHLCVDSHGNPLDFKVTGGNVHDSQVANDLIEVIQEAQ
                     YFIADKGYDSQEIRDKAIEHGMKAIIPKRKNAKKPNPDFDSYLYKLRHLVENAFARLK
                     QFRSIATRYEKLARNFKSMLYLACSIIHAKLN"
BASE COUNT          185 a          172 c          148 g          256 t
ORIGIN      
        1 ggcgtgtcct caatttaact tagcatgaat aatagagcaa gccaagtaaa gcatcgattt
       61 aaaattacga gctaatttct catagcgagt agcaatacta cgaaactgct ttaatcgtgc
      121 aaatgcattc tcgactaagt ggcgtaattt ataaaggtag ctatcaaaat cagggttagg
      181 tttcttggca tttttacgct ttggtataat agctttcata ccgtgttcta tcgctttatc
      241 tctgatttct tgcgagtcat accctttgtc agcgataaaa tactgggctt cttgtatgac
      301 ttctatcaag tcgtttgcaa cttgactgtc gtgcacgtta cccccagtga ctttaaaatc
      361 gagcggattt ccatgcgagt ccacacatag gtgtattttt gtcgtgtttc cgccacggct
      421 ttgtccaatt gctctatcga aaccacgccg agctccactt gcatgttgat gacaccgtac
      481 ataacttccg tcgatgaata cccattcttt gtcaatttct tttcgtagat caaaaaaaaa
      541 ttctgccaca agcctttttt agaccatcgg ttaaagcggt tataagcggt tttccatgac
      601 cctagctcaa taggtatgtc tcgccatggt gcacctgttc ttagcttcca tagtatggct
      661 tccatcacgg tacggtcgtt cttccattga tgacagccgt gcgctttcat ggttgtttgt
      721 aattgttccc atatgttgtc agttattgcg gttctcgcca t
//
LOCUS       DAXHSF010000265          760 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.265, whole genome shotgun sequence.
ACCESSION   DAXHSF010000265 DAXHSF010000000
VERSION     DAXHSF010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.265"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(<1..216)
                     /locus_tag="ACFM8S_002047"
     CDS             complement(<1..216)
                     /locus_tag="ACFM8S_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC6315050.1"
                     /translation="MKITHCKLKKKVQKELLRFFVLEVTARSAADILGIHPNSAALFY
                     RKIRTVINHHLALAADEVFEGPVEQDES"
     gene            complement(336..>760)
                     /locus_tag="ACFM8S_002048"
     CDS             complement(336..>760)
                     /locus_tag="ACFM8S_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6315051.1"
                     /translation="GGYRSGTSEKGFQAVFGRVVTVENVIRLPEKFGRFSGSLWYWAQ
                     QTQVQIRRCAVIVRMPIQPKQTSVFRKWMFDGAKVVRRMEGNLWVFGMVALEKTCFKG
                     QMPSENRFQTAFSVYLKQTGKGSNILQSSYSHKRFIVN"
BASE COUNT          202 a          194 c          142 g          222 t
ORIGIN      
        1 gctttcgtcc tgctcgacag ggccctcaaa aacctcatcg gcagccaagg ctaaatgatg
       61 gttgataacc gtgcggattt tacggtagaa cagtgctgcc gaattgggat ggatacccaa
      121 aatatcggcg gcagaacggg cggtaacttc gagtacaaaa aaacggagca gttctttctg
      181 tacttttttc tttaatttgc agtgcgttat cttcatattt cgagggtaac atatctgcta
      241 atctagtaca gcccctagtt taaagctttg tttcttaagt ccgcagagta tgccatggtt
      301 agaccttcaa agttgagtat tgtactattt tgtttttaat taactataaa acgcttgtgt
      361 gaataggaag actgcagaat attgctgcct tttcctgttt gctttaaata aacagaaaat
      421 gccgtctgaa accgattttc agacggcatt tgtcccttaa aacacgtttt ttcaagcgcg
      481 accataccaa acacccacag gttgccttcc atccttcgta caacctttgc tccatcaaac
      541 atccacttcc taaatactga tgtctgctta ggttgaatcg gcattcgtac gattacggca
      601 caccgcctaa tctgtacctg cgtctgttgc gcccaatacc aaaggctacc tgaaaatctc
      661 ccaaactttt caggtagcct tatcacgttt tcgactgtca ccacacgccc aaagaccgcc
      721 tgaaaccctt tttcagacgt tccactccga taccccccaa
//
LOCUS       DAXHSF010000266          757 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.266, whole genome shotgun sequence.
ACCESSION   DAXHSF010000266 DAXHSF010000000
VERSION     DAXHSF010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.266"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(54..365)
                     /gene="rpsJ"
                     /locus_tag="ACFM8S_002049"
     CDS             complement(54..365)
                     /gene="rpsJ"
                     /locus_tag="ACFM8S_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFC6315052.1"
                     /translation="MANQKIRIRLKAYDYALIDRSAQEIVETAKRTGAVVKGPIPLPT
                     KIERFNILRSPHVNKTSREQLEIRTHLRLMDIVDWTDKTTDALMKLDLPAGVDVEIKV
                     Q"
     gene            complement(383..>757)
                     /locus_tag="ACFM8S_002050"
     CDS             complement(383..>757)
                     /locus_tag="ACFM8S_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFC6315053.1"
                     /translation="AGDNVGVLLRGTKREDVERGQVLAKPGTITPHTKFKAEVYVLSK
                     EEGGRHTPFFANYRPQFYFRTTDVTGAVTLEEGVEMVMPGENVTITVELIAPIAMEEG
                     LRFAIREGGRTVGAGVVSSVIA"
BASE COUNT          179 a          191 c          184 g          203 t
ORIGIN      
        1 taacataaaa aatccccaag cattgattgc ttggggattt tcaatagtcc gaattattgg
       61 actttgattt ctacatcaac accggccggc aaatccagct tcatcagcgc atcggtagtt
      121 ttatcggtcc aatccacgat gtccatcagg cgcaagtggg tgcggatttc caattgttca
      181 cgggaagttt tgttcacgtg cggagaacgc agaatgttga aacgttcgat tttggtcggc
      241 aacggaatcg gaccttttac aacggcaccg gtacgttttg cagtttcaac gatttcttgt
      301 gcagaacggt caatcagggc gtaatcataa gctttcaggc ggatacggat tttttggttt
      361 gccatttatc aatatccttc aattaagcga taacagaaga aaccacgccg gcacccacgg
      421 tacgaccgcc ttcgcgaatc gcaaagcgca ggccttcttc catagcgata ggcgcaatca
      481 gttctacggt aatggttacg ttctcacccg gcattaccat ttccacacct tcttccaaag
      541 taaccgcgcc ggttacgtcg gtggtacgga agtagaattg cggacggtag ttggcgaaga
      601 acggagtatg gcggccgccc tcttctttgc tcagtacgta cacttctgct ttgaatttgg
      661 tgtgaggagt gatagtaccc ggtttggcca atacctgacc gcgttccacg tcttcacgtt
      721 tggtaccgcg cagcaatacg cctacgttgt cgcccgc
//
LOCUS       DAXHSF010000267          753 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.267, whole genome shotgun sequence.
ACCESSION   DAXHSF010000267 DAXHSF010000000
VERSION     DAXHSF010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.267"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(30..428)
                     /locus_tag="ACFM8S_002051"
     CDS             complement(30..428)
                     /locus_tag="ACFM8S_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HFC6315054.1"
                     /translation="MCEFKDFRRNIPCFKEYDENSFIGKWYDDGVWDDEEYWKLENDL
                     IEVRRKYPYPMDIPRDIVIGIGSIIDFLMVQNWKLFEIKASPWLPKSVKINERYERFR
                     VMLRYIFTDLDVDDWKFFYFPIQHSKGRLR"
     gene            complement(434..753)
                     /locus_tag="ACFM8S_002052"
                     /pseudo
     CDS             complement(434..753)
                     /locus_tag="ACFM8S_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670623.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin C-terminal domain-containing protein"
BASE COUNT          215 a          172 c           93 g          273 t
ORIGIN      
        1 ataatgtatc tccaatataa ttaaactttt catctcaatc tacctttact atgttgtatt
       61 ggaaagtaaa aaaatttcca gtcatctaca tctagatcag taaaaatata acggagcatt
      121 actctgaacc tttcataacg ctcattaatt ttgacacttt taggcaacca aggggaagct
      181 ttaatttcaa aaagtttcca attttgaacc attaaaaaat caataatgct acctattcca
      241 atcacgatgt cccttggtat atccatcgga taaggatatt ttctcctaac ctcgattaaa
      301 tcattctcca atttccaata ttcttcatca tcccacaccc cgtcatcata ccatttgcca
      361 ataaatgaat tttcgtcata ctccttaaaa caagggatgt ttcttctaaa atccttgaac
      421 tcgcacatca taattaatct ccaatacgat ttaggttttt atcaaatgta ccgtttcttg
      481 tttcttttct gttcagcttc ttgggtgaag atgcttcttt ccaagcccct ccattatgtg
      541 aatctacatc gcgtgatata taactctttc cttttttaaa aatagcagca tcatttctcg
      601 ttctttcttt tatttttcta tatcccaatt tctttgctgc tgcatatgct tctgaatcat
      661 tcccatatac gggggtagat ggtgtttttc ttggcggaca cgcattatga acccaaaccc
      721 cttccgttcc gcctgattac ccttgacgaa gta
//
LOCUS       DAXHSF010000268          725 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.268, whole genome shotgun sequence.
ACCESSION   DAXHSF010000268 DAXHSF010000000
VERSION     DAXHSF010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.268"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            586..>725
                     /gene="pilE"
                     /locus_tag="ACFM8S_002053"
     CDS             586..>725
                     /gene="pilE"
                     /locus_tag="ACFM8S_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial major subunit PilE"
                     /protein_id="HFC6315055.1"
                     /translation="MNTLQKGFTLIELMIVIAIVGILAAVALPAYQDYTARAQVSEAI
                     LLA"
BASE COUNT          246 a          199 c          113 g          167 t
ORIGIN      
        1 acgtaaaatc taaagaaacc gttttcccga taagtttccg taccgacaga cctggattcc
       61 cgcctgcgcg ggaatgacga agccatccgc acggaaacct gccgcgatga ttcaactgcc
      121 gtttgcacag gaacaccacg cgccgatttc aatcactttc caagaaaacg gaaattttta
      181 aaaattaaat ttaaaatttc ccacccaacc caccctatgc taccgcgcaa attcaaaaat
      241 ctcaaattcc gacccaatca acacacccga tgccccatgc caatagaaat accccatgcc
      301 aataaaaaag tgccgaaaat cggcactaaa actgacaatt ttcaacacta ctaacaaaaa
      361 acggtaaccc cctgcttcct caaaccacgc ccaaacgaaa gaaaatgtaa aataaaaaca
      421 accatataaa gataaacgca taaattttca cctcaaaaca caaacttgat accaatcttg
      481 ctttataatg cacaggtatc gcaacaaaat accgatggtc aaatacattg cataatgccg
      541 atggcgtaag cttgaggcat ttcctttcca attaggagta attttatgaa cacccttcaa
      601 aaaggtttta cccttatcga gctgatgatt gtgattgcca tcgtcggcat tttggcggca
      661 gtcgcccttc ctgcttatca agactacaca gcccgcgcac aagtttccga ggccattctt
      721 ttggc
//
LOCUS       DAXHSF010000269          707 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.269, whole genome shotgun sequence.
ACCESSION   DAXHSF010000269 DAXHSF010000000
VERSION     DAXHSF010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.269"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(91..642)
                     /locus_tag="ACFM8S_002054"
     CDS             complement(91..642)
                     /locus_tag="ACFM8S_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6315056.1"
                     /translation="MKVLNGWSDRKMWRVLSALPIGVVFFDLIYGFVLNVLQGLDLQR
                     AVPDLEGVLAVTPDIAFNSLQIVANGGMAAVVCFGLAVVFLLNRSVRRRQVLEIGVFR
                     MLGLVAVLAFSAPSVWEWANALPLLLKGADVVNTGNARYVLTALCMPFPAVSCVIGLV
                     GRFRLQTASGRAAKSGGAGKADG"
BASE COUNT          199 a          277 c          133 g           98 t
ORIGIN      
        1 caaagaaacc gccattagca ctacaccccc ccaagctgcg gctgccaaac acatcggctg
       61 cttctcgacg cacccgctga aaaatgcgtc ctatccgtcc gccttgcccg caccccctga
      121 ctttgccgcc ctgcccgatg ccgtctgaag cctgaaccgc cccaccagcc cgatgacgca
      181 cgacaccgcc ggaaagggca tacacaaagc cgtcagcaca taacgcgcat tccccgtatt
      241 gaccacgtcc gcgcccttca gcagcagcgg tagcgcgttc gcccactccc acaccgacgg
      301 cgcgctgaac gccaataccg ccaccagccc caacatccgg aacaccccga tttccaacac
      361 ctgccgccgc cgcaccgaac ggttgagcaa aaacacaacc gccaacccga aacagaccac
      421 cgccgccata ccgccgttgg cgacaatctg caaactgttg aatgcaatat cgggcgtaac
      481 cgccaacacg ccttccaaat ccggcacggc acgctgcaaa tccaaaccct gcaacacatt
      541 caacacaaaa ccgtagatca aatcaaaaaa caccacgcct atcggcaaag cactcaaaac
      601 ccgccacatc ttcctgtccg accaaccgtt caaaaccttc attttacccg accacgcaag
      661 ccgccgaaca aaaaataagg ggctgtccta gataactagg acaaact
//
LOCUS       DAXHSF010000270          703 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.270, whole genome shotgun sequence.
ACCESSION   DAXHSF010000270 DAXHSF010000000
VERSION     DAXHSF010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.270"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(<1..385)
                     /locus_tag="ACFM8S_002055"
     CDS             complement(<1..385)
                     /locus_tag="ACFM8S_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFC6315057.1"
                     /translation="MAIELNGNWTKGFALDHHMQKSILLGYDELGHPHFDSKRSVIGE
                     WVYQLKYRNQTQNVSLLVDYILQKFSGLESINLIVPAPFTTERINQPVQLIAKELSDR
                     LNIPYSPILRKSSSHTPLKNIEEKSE"
BASE COUNT          192 a          162 c          132 g          217 t
ORIGIN      
        1 tttctgattt ttcttcaata tttttgagtg gtgtgtgtga gctagatttt cttaaaatag
       61 gagaatatgg aatattcaag cgatcactta gttctttggc aattaattga actggttgat
      121 taatacgctc tgttgtaaaa ggggcgggaa caattaaatt tatagattct aaaccactaa
      181 atttttgtaa aatatagtct accagcaaag aaacattttg ggtttgatta cgatatttta
      241 attgataaac ccattctcca atcacacttc tcttgctatc aaagtgagga tgccctaatt
      301 catcataacc taacaaaata cttttttgca tatgatggtc taaagcaaat ccttttgtcc
      361 agttaccatt tagttctatc gccatttcat tgactccttg tcaaaattaa gtataacgag
      421 caatcctagc aagccgtagc ccgcatgtag ggtatgtgcg gtacgcacgc acgcgttctt
      481 cattttccct gtaaccccaa cccaccgcat gcgcgccttg cggcacacac tctgccgacg
      541 gattcaaagg tcgtctgaaa aaacgctcgc ggcgtttgtt acatctgcct tgcgataaag
      601 gctgcctgaa aaattcccac tgttgatatt tcgggcattc tttctcaaac cgcctgcaac
      661 cgcaaaatcc gctgcaacct gccccctccc ccgtggggga ggg
//
LOCUS       DAXHSF010000271          687 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.271, whole genome shotgun sequence.
ACCESSION   DAXHSF010000271 DAXHSF010000000
VERSION     DAXHSF010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.271"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(37..687)
                     /locus_tag="ACFM8S_002056"
                     /pseudo
     CDS             complement(37..687)
                     /locus_tag="ACFM8S_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247434.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS1655 family transposase"
BASE COUNT          108 a          152 c          208 g          219 t
ORIGIN      
        1 ggtgttctca ttcagccctt tctcccaaga atgctaaggg cgacaaaaat aagtctccgc
       61 tttcaatgct ttggttattt tggtgtgttg gtagaactct ttgccgttat ccatggtaat
      121 ggtgtgcacc ctgtctttat gtgcctttaa tgccctaaca gctgcccggg cagtgtcttc
      181 ggctttgagg ctatccaatt tgcagatgat ggtgtagcgg gtaacgcgtt cgaccaaggt
      241 caataatgcg cttttctgtc ctttgccgac aatggtgtcg gcttcccaat cgccgatacg
      301 ggatttctgg tcgacgatag cgggtcggtt ttctatgccg acacggttgg gtactttgcc
      361 tctggtccat gtgctgccgt agcgtttgcg gtagggtttg ctgcatattc tgagatgttg
      421 ccacaacgtg ctgccgttgc ttttgtcttg gcgaaggtag cggtaaatgg tgctgtggtg
      481 gagcgtgatc cggtggtgtt tgcacaggta ggcgcatact tgttcgggac tgagtttgcg
      541 gcggataagg gtgtcgatgt gctgaatcag ctgcgaatcg agcttatagg gttgtcgctt
      601 acgctgtttg atagtccggc tttgccgctg ggctttttcg gcgctgtatt gctgcccttg
      661 ggtgcggtgc cgtctgattt cgcggct
//
LOCUS       DAXHSF010000272          670 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.272, whole genome shotgun sequence.
ACCESSION   DAXHSF010000272 DAXHSF010000000
VERSION     DAXHSF010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.272"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(129..>670)
                     /locus_tag="ACFM8S_002057"
     CDS             complement(129..>670)
                     /locus_tag="ACFM8S_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFC6315058.1"
                     /translation="SAVESRPAVPAAVQTPVKPAAQPPVQSAPQPAAPAAENKAVPAP
                     APQSPAASPSGTRSVGGIVWQRPTQGKVVADFGGNNKGVDIAGNAGQPVLAAADGKVV
                     YAGSGLRGYGNLVIIQHNSSFLTAYGHNQKLLVGEGQQVKRGQQVALMGNTDASRTQL
                     HFEVRQNGKPVNPNSYIAF"
BASE COUNT          151 a          193 c          186 g          140 t
ORIGIN      
        1 ccaaacacag tcaaacaaat ttatatgccc catcccttcc gaataatttg aaaacacagc
       61 cgccaaaaac aaaaatgccg tctgaaaacc tttcagacgg catttccaat ttgatttcag
      121 gcagaaagtc agaacgcgat atagctgttc gggttaaccg gtttgccgtt ttgccgcacc
      181 tcgaaatgaa gctgcgttct ggaagcgtcg gtattgccca tcaaagcgac ctgctgcccg
      241 cgtttgacct gctggccttc gccgaccagc aatttttggt tgtgcccgta tgcggtcagg
      301 aaggaagaat tatgctggat gatgaccaga ttgccgtatc ccctcaaacc ggaacctgca
      361 taaaccactt tgccgtcagc cgccgccaaa acgggctgtc ccgcattacc ggcaatatcg
      421 acacccttgt tgttgccgcc gaaatcggca accactttac cttgcgtcgg acgctgccaa
      481 acaatgccgc cgaccgaacg cgtgccggaa ggcgaagcgg caggagattg cggggcgggc
      541 gcgggaaccg ctttattttc cgcagcgggc gcggcaggtt gcggcgcgga ctgcacaggc
      601 ggttgcgcgg cgggtttcac aggggtttgc acggcagccg gtacggcggg cctgctttct
      661 acggctgagg
//
LOCUS       DAXHSF010000273          649 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.273, whole genome shotgun sequence.
ACCESSION   DAXHSF010000273 DAXHSF010000000
VERSION     DAXHSF010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.273"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(49..534)
                     /locus_tag="ACFM8S_002058"
     CDS             complement(49..534)
                     /locus_tag="ACFM8S_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFC6315059.1"
                     /translation="MRFFGIGFLVLLFLEIMSIVWVADWLGGGWTLFLMAAGFAAGVL
                     MLRHTGLSGLLLAGAAMRSGGRVSVYQMLWPIRYTVAAVCLMSPGFVSSVLAVLLLLP
                     FKGGAVLQAGGAENFFNMNQSGRKEGFSRDDDIIEGEYTVEEPYGGNRFRNAIEHKKD
                     E"
BASE COUNT          195 a          211 c          115 g          128 t
ORIGIN      
        1 aaaccggaaa aatgccgtct gaatcttcag acggcattcc attcatattt attcgtcttt
       61 tttgtgttcg atggcgtttc ggaaacgatt gccgccgtaa ggctcttcaa ccgtatattc
      121 cccctcgata atatcgtcat cgcgggaaaa gccctctttt ctgcccgatt ggttcatgtt
      181 gaaaaaattt tccgcacctc ctgcctgcaa cactgcacct cccttaaacg gcagcagcag
      241 caacaccgcc aacaccgagg atacgaatcc cggactcatc agacacaccg ccgccaccgt
      301 ataacggata ggccacaaca tctgataaac ggataccctc ccgccgcttc tcattgccgc
      361 gcccgccaat aaaagaccgg acagccccgt atgcctgagc atcagcacac cggcggcaaa
      421 acctgccgcc atcaaaaaca acgtccagcc gccgcccaac caatcggcaa cccacacaat
      481 cgacataatc tccaaaaaca gcagcaccaa aaaaccgata ccgaaaaatc tcattgaccg
      541 tcatccttat atttaagtaa acagcaaacc gcccgaacag gactccaagc gacctgcctg
      601 taaatgatta caaaaccatg tgcttcaagc cgaaacaatg tgaaatcac
//
LOCUS       DAXHSF010000274          649 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.274, whole genome shotgun sequence.
ACCESSION   DAXHSF010000274 DAXHSF010000000
VERSION     DAXHSF010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.274"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(49..366)
                     /locus_tag="ACFM8S_002059"
     CDS             complement(49..366)
                     /locus_tag="ACFM8S_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HFC6315060.1"
                     /translation="MNKPFITQAQLALYKYQPSSKYFGQSMAVIAQSEFVEFAKINKS
                     ENVIDCFSFFWNRRIKHDIWLISFSDNSEMVIKESLKDGHKIYKFEFCEIVDNCNFDD
                     VFV"
BASE COUNT          210 a          142 c          103 g          194 t
ORIGIN      
        1 gtatcaactg aaacagtttc agatattttt actgcatttg cattcgcttc aaacgaatac
       61 atcatcaaaa ttgcaattat cgacaatttc gcaaaattca aatttgtata ttttatgacc
      121 atctttcagg gattctttaa ttaccatttc tgaattatca gaaaatgaga ttagccaaat
      181 atcatgttta attcttctat tccagaaaaa agagaaacaa tcaataacat tttcagactt
      241 attaatcttc gcaaattcaa caaattcaga ttgcgctata accgccatcg attgcccaaa
      301 atacttgctt gacggctgat atttataaag tgccaactgc gcctgagtga taaacggctt
      361 gttcatggtt ctgcctttca atgattgttt tgaaagcctg attttgacac cataacttca
      421 tgcgctcaat tcttaaacag aaccgccccg attaatacgg gtacggaaac gccgagataa
      481 aaataaaaat ccatcatttc aaaacctttt tcagcaggga aacaaagtaa acggacgcga
      541 ggatgccgaa tactatccag cctgtttcaa gaccgctttg caggttgtct ttcggactgc
      601 attccgccaa taaaagcctt agcggctgac cgtccgacat cttccacag
//
LOCUS       DAXHSF010000275          563 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.275, whole genome shotgun sequence.
ACCESSION   DAXHSF010000275 DAXHSF010000000
VERSION     DAXHSF010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.275"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            21..>563
                     /locus_tag="ACFM8S_002060"
     CDS             21..>563
                     /locus_tag="ACFM8S_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC6315061.1"
                     /translation="MQADLAYAAERITHDYPEATGANNTSTVSDYFRNIRTHSIHPRV
                     SVGYDFGGWRIAADYARYRKWNNNKYSVNTKNVQVHKSNGNREDLKTENQENGTFHAV
                     SSFGLSAVYDFDTGSRFKPYAGVRVAYGHVRHSIDSTKKTTNVLTVPSNIPGGTPTIY
                     NQGSTQNAYHESHSIRRLGLG"
BASE COUNT          165 a          159 c          129 g          110 t
ORIGIN      
        1 gcagccgcag cccgtattat gtgcaggcgg atttagccta cgccgccgaa cgcattaccc
       61 acgattatcc ggaagcaacc ggtgcaaaca acacaagcac agtaagcgat tatttcagaa
      121 acatccgtac gcattccatc cacccccggg tgtcggtcgg ctacgacttc ggcggctgga
      181 ggatagcggc agattatgcc cgttacagaa aatggaacaa caataaatat tccgtaaaca
      241 caaaaaatgt gcaggtgcat aaaagcaatg gcaacaggga agacctgaag acggaaaatc
      301 aggaaaacgg tacattccac gccgtctcct cgttcggctt gtccgccgtt tacgatttcg
      361 ataccggttc ccgcttcaaa ccctatgcag gcgtgcgcgt cgcctacgga cacgttagac
      421 acagcatcga ttcgaccaaa aaaacaacaa atgttcttac cgtccccagc aatattcctg
      481 gcggaacacc tacgatttat aatcagggaa gtacgcaaaa cgcctatcac gaaagccaca
      541 gcatccgccg cttgggtctt ggt
//
LOCUS       DAXHSF010000276          555 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.276, whole genome shotgun sequence.
ACCESSION   DAXHSF010000276 DAXHSF010000000
VERSION     DAXHSF010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.276"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
BASE COUNT          184 a          100 c           76 g          195 t
ORIGIN      
        1 tagaaattag ttaaagaata tattatggta gatactaact ctttagttaa aaataattta
       61 ctaactttta ctacttaggt ataaattcca atacttaact aaactgaggg taaatatatt
      121 tatccaagat agaagagaaa cttttttctc tgtttccgca agaatagtta cgggttgtga
      181 aatatgctct atacccaagc agtagatatt ttcattgtta cttaccgtat tgcttcatag
      241 aattggcact ctacgctttc ctttaaaaat cgtcaataac gattatcatt ttctttaaat
      301 ttattcaaaa caatattctt atacacatct caagcccgct gaaataaagc aaaatggtgg
      361 ttcatcgact gtaatgccaa tttcaaagca gcctgcactt tgacaacaca caagatatac
      421 gggaaggaca gggcatcagc atatatttca gcccgaagcc gtttcattcg tttaaaaaac
      481 tgcttttttc acaatttacc tttacaaagc ctatacctat ccttacaatt tgttttgctg
      541 tttttggtat atttt
//
LOCUS       DAXHSF010000277          548 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.277, whole genome shotgun sequence.
ACCESSION   DAXHSF010000277 DAXHSF010000000
VERSION     DAXHSF010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.277"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..>548
                     /locus_tag="ACFM8S_002061"
     CDS             <1..>548
                     /locus_tag="ACFM8S_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility protein"
                     /protein_id="HFC6315062.1"
                     /translation="DAGDVYCVYNIRLKQYTACQVTRVEEHGGKKAYATLLALDWQGN
                     KPLGAAELADLKPLYKDFMYWERGLHMYKASAVVPTGYVRVGNTAPLVGEDTQRYASF
                     WGDGYDVYRQLRWQQIPEKQRKAFKKAAKSKKTVMFAGREYGISKQNLSDVWDDFEDA
                     MELKAFPCLSSLFLTKWHKNLY"
BASE COUNT          151 a          121 c          158 g          118 t
ORIGIN      
        1 ctgatgccgg agacgtatat tgcgtctata acatcagact gaagcagtac actgcctgcc
       61 aggttacccg agttgaagaa cacggtggga aaaaagcata tgcaaccctg ctggctttgg
      121 actggcaggg caacaaaccg cttggggcgg cggagctggc ggatttgaaa ccgctttaca
      181 aagactttat gtattgggaa cgcggtctgc acatgtataa ggcaagtgcc gtcgtgccga
      241 cgggatatgt acgggttggg aataccgcgc cgctggtcgg cgaagacacg caacggtatg
      301 cctctttttg gggcgacggc tacgacgtgt accgtcagtt gagatggcag cagatacccg
      361 aaaaacagag aaaggcattc aaaaaagccg ccaaaagcaa aaagaccgtg atgtttgccg
      421 gacgggaata cggcatatcc aaacagaatt tgagcgatgt ttgggatgat tttgaagacg
      481 cgatggaact gaaggcgttt ccctgcctgt cttcgctgtt tctgaccaaa tggcataaaa
      541 atctatat
//
LOCUS       DAXHSF010000278          528 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.278, whole genome shotgun sequence.
ACCESSION   DAXHSF010000278 DAXHSF010000000
VERSION     DAXHSF010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.278"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..309
                     /locus_tag="ACFM8S_002062"
     CDS             <1..309
                     /locus_tag="ACFM8S_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
                     /protein_id="HFC6315063.1"
                     /translation="KFVPKNSKTYSSDLIKTVDLTHIPTGAKARINAKITASISRAGV
                     LAGGGKLARLGAKFSTRAVPYVGTALLAHDVYETFKEDIQARGYQYDTETDKFAKVSG
                     "
BASE COUNT          165 a          117 c          136 g          110 t
ORIGIN      
        1 aagtttgtac ctaaaaatag taaaacttat tcatctgatt taataaaaac ggtagattta
       61 acacacatcc ctacgggcgc aaaagcccga atcaacgcca aaataaccgc cagcatttcc
      121 cgcgccggcg tattggcggg gggcggcaaa cttgcccgct taggcgcgaa attcagcaca
      181 agggcggttc cctatgtcgg aacagccctt ttagcccacg acgtatacga aactttcaaa
      241 gaagacatac aggcacgagg ctaccaatac gacaccgaaa ccgacaaatt tgcaaaggtc
      301 tcaggctaag tgcgcctgtt gccgcctaaa aagtaacccg gatgcctgat tatcgggtat
      361 ccggggagga ttaagggggt atttaggtaa aattaggagg tgttatggag cgaaaacagc
      421 cgaaaacctg tgtttgggtt tcggctgtcg ggagggaaag gaattttgca aaggtctcaa
      481 ttagtatagt ggattaacaa aaatcaggac aaggcgacga agccgcag
//
LOCUS       DAXHSF010000279          525 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.279, whole genome shotgun sequence.
ACCESSION   DAXHSF010000279 DAXHSF010000000
VERSION     DAXHSF010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.279"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..78
                     /locus_tag="ACFM8S_002063"
     CDS             <1..78
                     /locus_tag="ACFM8S_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC6315064.1"
                     /translation="YHNWGRLENTRFKTHEVSLGMRYRF"
BASE COUNT          133 a          165 c          117 g          110 t
ORIGIN      
        1 taccacaact ggggacgctt ggaaaacacc cgcttcaaaa cccacgaagt ctcattgggc
       61 atgcgctacc gcttctgatt ccccgacacc gatgccgtct gaaccttcag acggcatttt
      121 tgatgcactc gccgtttaca gacgcggggc gggcgtggaa atacccaaac cgtcattccc
      181 gacaacaccg caatctcgaa attcgtcatt cccgcgcagg cgggaatcca gacccccgac
      241 gcggcgggaa tctatcggaa atgactgaaa ccccgagatt ctagattccc actttcgtgg
      301 gaatgacggt tcagttgcgt tccgacaaca ccgcaatctc gaaattcgtc attcccgcgc
      361 aggcgggaat ccagacctgt ccgcacagaa acttatcgga taaaaacagt tgctcaaacc
      421 cctcgttcta gattcccact tccgtgggaa tgacggttca gttgcgttcc gacaacaccg
      481 taatctcgaa acccgtccga caacaccgta atcttgaaat tcgtc
//
LOCUS       DAXHSF010000280          524 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.280, whole genome shotgun sequence.
ACCESSION   DAXHSF010000280 DAXHSF010000000
VERSION     DAXHSF010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.280"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            461..524
                     /locus_tag="ACFM8S_002064"
                     /pseudo
     CDS             461..524
                     /locus_tag="ACFM8S_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
BASE COUNT          163 a          160 c          102 g           99 t
ORIGIN      
        1 caaagccgac agcgacaccg tcgccgccgc caccggcacc gacgccgcca agaagatcga
       61 caccaagcac ctgccgtcaa cctgccgcga cgcagcatct gtcgtttgca tagaaacacc
      121 acctacggct ttctataaaa atacctaaac cgtcattccc acgaaagtgg gaatccagaa
      181 cgcaaaatct caagaaaccg ttttcccgat aagtttccgt accgacagac ctagattccc
      241 gcctgcgcgg gaatgacggc ggagcggttt ctgttgctcc cgataaattc ctaaaactca
      301 aaatttcatc attccctcaa aaacagaaaa ccaaaaacag caacctaaaa ttcatcattc
      361 ccacgaaagt gggaatccag aacgcaaaat ctaaagaaac cgttttaccc gataagtttc
      421 cgtaccgaca gacctggatt cccgcctgcg cgggaatgac gaagctatcc ttttggctga
      481 aggtcaaaaa tcagccgtca ccgagtatta cctgaatcac ggca
//
LOCUS       DAXHSF010000281          517 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.281, whole genome shotgun sequence.
ACCESSION   DAXHSF010000281 DAXHSF010000000
VERSION     DAXHSF010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.281"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            237..>517
                     /locus_tag="ACFM8S_002065"
     CDS             237..>517
                     /locus_tag="ACFM8S_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6315065.1"
                     /translation="MFPDKYKLSLEENIFLAKKVLVAQIHNLSRFENCQTTLLQTEQI
                     IHGKNVASASLEDIQTILNLKRAYQYVISHISNGEPVNISLLKKINNIVA"
BASE COUNT          166 a          107 c           99 g          145 t
ORIGIN      
        1 ttttgcaaag gtctcaaacg gatgaaatca accgatattc aaacacagtc atttttagcg
       61 cattttcagc gtatcgttaa tgcggaaaat ttcgtgaaca ggttttttgc acaggtctcc
      121 gcctcgtttc gcgggatggg aaaccgtatt agaaaacgga cgcacgcaac ataaaccccg
      181 aaagtgatga taagatgatg atttaacgta ctgctttaat tatttaagga attatcgtgt
      241 ttcccgacaa atacaagttg agtttggaag aaaatatttt tctggcaaag aaagtattgg
      301 ttgcccaaat tcacaacctc agccgttttg agaattgtca gacgaccttg ttgcagaccg
      361 aacaaattat ccatggcaaa aatgtagcct ccgcgtcact ggaagacatc caaaccatct
      421 tgaacctgaa acgtgcctat caatatgtga tttcgcatat ttcaaatggc gaaccggtca
      481 atatttcact ccttaaaaaa atcaacaaca ttgttgc
//
LOCUS       DAXHSF010000282          516 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.282, whole genome shotgun sequence.
ACCESSION   DAXHSF010000282 DAXHSF010000000
VERSION     DAXHSF010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.282"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..370
                     /locus_tag="ACFM8S_002066"
     CDS             <1..370
                     /locus_tag="ACFM8S_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6315066.1"
                     /translation="AHECNHLLPLLEGIAEGTTVYADKGYDSAENRQHLEERQLLDGI
                     MRKAHRNRPLTEVQTKHNRYLSKTRYVVEQSFGTLHRKFRYARAAYFGLIKVSAQSHL
                     KAMCLNLLKAANRLSAPAAA"
BASE COUNT          133 a          117 c          156 g          110 t
ORIGIN      
        1 tgcccatgag tgcaaccacc tgttgccttt gctggaaggt atagccgaag gcacgaccgt
       61 ctatgccgac aaaggctatg acagtgcgga aaaccggcaa catctggaag agcgtcagtt
      121 gttagacggc attatgcgca aagcccaccg caaccgtccg ctgacggaag tgcaaaccaa
      181 acataaccgg tatttgtcga agacccgtta tgtggtcgaa caaagcttcg gtacgctgca
      241 ccgtaaattc cgctatgcgc gggcggccta tttcggactg attaaagtga gtgcgcaaag
      301 ccatctgaaa gcgatgtgtt tgaacctttt gaaagcggcc aacaggctaa gtgcgcccgc
      361 tgccgcctaa aaggcgaccg gatgcctgat tatcgggtat ccggggagga ttaagggggt
      421 atttgggtag aattaggagg tatttggggc gaaaacagcc gaaaacctgt gttggggttt
      481 cggctgtcgg gagggaaagg aattttgcaa aggtct
//
LOCUS       DAXHSF010000283          493 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.283, whole genome shotgun sequence.
ACCESSION   DAXHSF010000283 DAXHSF010000000
VERSION     DAXHSF010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.283"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            302..>493
                     /locus_tag="ACFM8S_002067"
     CDS             302..>493
                     /locus_tag="ACFM8S_002067"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFC6315067.1"
                     /translation="MTMQVFLTIPGYDVEAEIEKFVWMDAVIWQMPGWWMHEPWTVKK
                     YIDGVLTAGHGKLYQSDGRH"
BASE COUNT          145 a          123 c          120 g          105 t
ORIGIN      
        1 actcattagt tacctaaaac ttaaaaagca gaaaccttta cgccgtcatt cccacgaaag
       61 tgggaatccg ggaacttaac gttacagtga tttatcggaa acggctgaaa ccgaatgaat
      121 tggattcccg cctgcgcggg aatgacgaat ccatccgtac ggaaacctgc accacgtcat
      181 tcccacgaac ctacattccg tcattcccac gaaagtggga atccaggacg aaaaatctcc
      241 agaaaccgtt ttacccgata agtttccgca ccgacaggtc tggattcccg cctgcgcggg
      301 aatgacgatg caggtatttc tgacgattcc cggctatgat gttgaggcag aaatcgaaaa
      361 attcgtttgg atggatgctg tgatttggca gatgccgggc tggtggatgc acgagccttg
      421 gacagtgaaa aaatacatag acggagtatt aaccgctgga cacggcaaac tctaccaaag
      481 cgacggcaga cac
//
LOCUS       DAXHSF010000284          461 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.284, whole genome shotgun sequence.
ACCESSION   DAXHSF010000284 DAXHSF010000000
VERSION     DAXHSF010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.284"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..182
                     /locus_tag="ACFM8S_002068"
     CDS             <1..182
                     /locus_tag="ACFM8S_002068"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6315068.1"
                     /translation="GNHGLGGLLEEGAHEKSPKCLGGNLGDFGEFCKGLYLYCLRLAA
                     LSLFKFNSLYLPVRL"
     gene            231..410
                     /locus_tag="ACFM8S_002069"
     CDS             231..410
                     /locus_tag="ACFM8S_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6315069.1"
                     /translation="MFWYVIGFCAFVVALLSLWVNAGAFGMQEDDTPQSDYERRLGLG
                     AKLKNKNPDTDEKRQ"
BASE COUNT          108 a           83 c          130 g          140 t
ORIGIN      
        1 ttggcaatca tggcttgggc ggtttgttgg aagaaggtgc tcatgagaaa tcccctaaat
       61 gtcttggtgg gaatttaggg gattttgggg aattttgcaa aggtctctat ttgtactgtc
      121 ttcggctcgc cgccttgtcc ttatttaaat ttaattcact atatttgccc gtgcgtttgt
      181 gataaattcg gcactgtatt tgatatgatt ttaacaaatc gggaggggca atgttttggt
      241 atgtgattgg cttttgcgcc tttgtcgtcg cgctgctgtc gctgtgggtc aatgccggcg
      301 cgttcggtat gcaggaagac gataccccgc aatcggatta tgaaaggcgt ttgggcttgg
      361 gtgcgaagct gaaaaacaaa aatccggata cggacgaaaa acggcaataa agacaacaaa
      421 tgccgtctga aaggttttca gacggcattt gctttggagc g
//
LOCUS       DAXHSF010000285          456 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.285, whole genome shotgun sequence.
ACCESSION   DAXHSF010000285 DAXHSF010000000
VERSION     DAXHSF010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.285"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..>456
                     /locus_tag="ACFM8S_002070"
     CDS             <1..>456
                     /locus_tag="ACFM8S_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6315070.1"
                     /translation="KDVNKNLGNGNTLAQQGSYTKTDGTTAKMGDLLLAADNLHSRFK
                     DKVELTAEQAKAANLAGIGRLRDLREAAALSGDLANMLKAYSAAETKEAQLALLDNLI
                     HKWAETDSNWGKKSPMRLSTDWTQTANEGIALTPSQVAQLKKNALVSLSD"
BASE COUNT          145 a          108 c          104 g           99 t
ORIGIN      
        1 aaagatgtaa ataaaaatct cggtaacggt aacactttgg ctcagcaagg cagctatacc
       61 aaaacagacg gtacaaccgc aaaaatgggg gatttacttt tagcagccga caatctgcac
      121 agccgcttca aagacaaagt ggaactcact gccgaacagg caaaagccgc caatcttgcg
      181 ggcattggtc gtctgcgcga tttgcgcgaa gctgccgcat tgtccggcga tttggccaat
      241 atgctgaaag cttattctgc cgccgaaact aaagaagcac agttggcatt gttagataat
      301 ttgattcaca aatgggcgga aaccgattcg aactggggca aaaaatcgcc aatgcgactt
      361 tcaaccgatt ggacgcaaac ggctaatgaa ggtattgcac tgacaccatc ccaagtagca
      421 caactaaaaa agaacgcttt agtttccctt tctgat
//
LOCUS       DAXHSF010000286          446 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.286, whole genome shotgun sequence.
ACCESSION   DAXHSF010000286 DAXHSF010000000
VERSION     DAXHSF010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.286"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
BASE COUNT          119 a          143 c          103 g           81 t
ORIGIN      
        1 tcccgacaac accgcaatct cgaaattcgt cattcccgcg caggcgggaa tccagcccca
       61 cgacgcggcg ggaatctatc ggaaatgact aaaaccccga gattctagat tcccacttcc
      121 gtgggaatga cggttcagtt gcgttccgac aacaccgcaa tcttgaaacc cgtccgacaa
      181 caccgtaatc ttaagacccg tcattcccgc gcaggtggga atccagaccc acgacgcggc
      241 gggaatctat cggaaatgac tgaaaccccg agattctaga ttcccacttc cgtgggaatg
      301 acggttcagt tgcgctccga caacaccgca atcttgaaac ccgtccgaca acaccgtaat
      361 cttaaaaccc gtcattcccg cgcaggtggg aatccagacc ccctgacgcg gcgggaatct
      421 atcggaaatg actgaaaccc cgagat
//
LOCUS       DAXHSF010000287          435 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.287, whole genome shotgun sequence.
ACCESSION   DAXHSF010000287 DAXHSF010000000
VERSION     DAXHSF010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.287"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
BASE COUNT          127 a           87 c          114 g          107 t
ORIGIN      
        1 ggattttgga tgatgtgcat ttatcgggaa aactaaaaac cctccgccgt cattcccgtg
       61 aaagcgggaa tctagaattt caatgccgca agaatttatc ggggatgtcc ggggtttaaa
      121 ggtctggatt cccgcctgca cgggaatgac ggtttagaaa tcacccgaaa cgagaaaaag
      181 aaccgaaacc ggataagtcg gattcccgca cgggagggaa ggacgggatt tcaggttact
      241 gtttttgatt ttctgttttg tgggaataac gggattttgg atgatgtgca ttttcgggaa
      301 aactaaaaac cctccgccgt cattcccgcg aaagcgggaa tctagaattt taatgcggca
      361 aaaatttatc ggaaaaaacc gaagtttaaa gacctagatt cccgcctgcg cgggaatgac
      421 ggtttagaaa tcacc
//
LOCUS       DAXHSF010000288          432 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.288, whole genome shotgun sequence.
ACCESSION   DAXHSF010000288 DAXHSF010000000
VERSION     DAXHSF010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.288"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..>432
                     /locus_tag="ACFM8S_002071"
     CDS             <1..>432
                     /locus_tag="ACFM8S_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6315071.1"
                     /translation="WLAQDNTLSELLKLINRQLTEKGLKIEKASAAVVDATIIQTAGS
                     KQRQAIEVDEEGQVSGQTTPSKDSDARWIKKNGLYKLGYKQHTRTDEEGYIEKLHITP
                     ANAHECTHLSPLLEGIAEGTTVYADKGYDSAENRQHLKEHQL"
BASE COUNT          143 a          115 c          102 g           72 t
ORIGIN      
        1 tggctggctc aagacaatac cctgtccgaa ttgctcaaac tgattaaccg acaactgacc
       61 gaaaaaggcc taaaaataga gaaagcatcc gccgccgttg ttgacgccac cattattcag
      121 accgccggca gcaaacagcg tcaggccata gaagttgacg aagaaggaca agtcagcggc
      181 caaaccacac cgagtaagga cagcgatgcc cgttggataa agaaaaacgg cctctacaaa
      241 ctcggttaca aacaacatac ccgtaccgat gaggaaggct atatcgagaa actgcacatt
      301 actcccgcca atgcccatga gtgcacacac ctgtcgccgt tgttggaagg gatagctgaa
      361 ggtacgaccg tctatgccga caaaggctat gacagtgcgg aaaaccggca acatctgaaa
      421 gaacatcagt ta
//
LOCUS       DAXHSF010000289          427 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.289, whole genome shotgun sequence.
ACCESSION   DAXHSF010000289 DAXHSF010000000
VERSION     DAXHSF010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.289"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(<1..314)
                     /locus_tag="ACFM8S_002072"
     CDS             complement(<1..314)
                     /locus_tag="ACFM8S_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6315072.1"
                     /translation="MKYRRFYRNGGTYFFTVVTNKRQKILTDDAVRLALRQAVMAVRE
                     RYPFEILAWVLMPDHLHTIWRLPDNDSAYSERWRQIKRHSQYLIGGNLRLWQKRFWEY
                     TIR"
BASE COUNT          114 a           97 c          100 g          116 t
ORIGIN      
        1 cggatagtat attcccaaaa gcgtttttgc caaagcctga gattgccgcc gattaaatat
       61 tggctgtgcc gcttgatttg ccgccagcgt tccgaataag cagaatcatt gtccggcagc
      121 cgccatatgg tatgcagatg gtcgggcatc aacacccatg ccaaaatttc aaacggatac
      181 cgttcgcgca ccgccattac cgcctgccgt aaagccaaac gcaccgcatc atcggtcaaa
      241 atcttctgcc gtttattggt tacaaccgta aaaaagtaag tgccgccatt gcggtaaaaa
      301 cgacggtatt tcatagtatt atgctcggaa tgattttgta ggtcggattc ttgaattcga
      361 cattttgggc attgctgcaa tggattgcaa tgatgggaat gttaaaggtt ttgtcggata
      421 caagtat
//
LOCUS       DAXHSF010000290          425 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.290, whole genome shotgun sequence.
ACCESSION   DAXHSF010000290 DAXHSF010000000
VERSION     DAXHSF010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.290"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
BASE COUNT          129 a          116 c           83 g           97 t
ORIGIN      
        1 tgttaatcca ctatatccgt ttgcgcccgc caagccgtgt tgcacagatg cgacacggct
       61 tttttctttc tgttgccaga atgtaaattt tcgtcaaaaa acatcaaaaa tcaccccccc
      121 cccgaatttg cgtaaaataa agtgtaatcc ctttgatcat aagggaatgt tgacaattag
      181 atcgattctc ctaggctgac tttcgcaata aatggggttg tcaataattg tgtatggtcg
      241 tttgccgtcc ggcggccgtc attcccgcga acgcaggaat ccaaaaacgc aacgcaacag
      301 gaatttatca gaaaaaacag aaaccccacc gaccgtcatt cccgcaaaag cgggaatcca
      361 gaaacacaac gcggcaggac tttatcggaa aaaacagaaa cccccaccac cgtcattccc
      421 gcgca
//
LOCUS       DAXHSF010000291          421 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.291, whole genome shotgun sequence.
ACCESSION   DAXHSF010000291 DAXHSF010000000
VERSION     DAXHSF010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.291"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            1..421
                     /locus_tag="ACFM8S_002073"
                     /pseudo
     CDS             1..421
                     /locus_tag="ACFM8S_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          113 a          143 c           82 g           83 t
ORIGIN      
        1 caagccatga ttgccaaaca tatcaaccgt ttcccgctat tgaagttgga ccaagtgatt
       61 gattggcagc cgatcgaacc atacctgaac cgtcaaagaa cccgttacct ccgagaccac
      121 cgcggtcgtc ccgcctatcc cctgctgtcc atgttcaaag ccgttctgct cggacaatgg
      181 cacagcctct ccgatcccga actcgaacac agcctcatca cccgcatcga cttcaacctg
      241 ttttgacgtt ttgacgaact gagcatcccc gattacagca ccttatgccg ctaccgcaac
      301 cggcgacgca agacgacacc ctgtccgaat tgccggaact gatcaaccgc caactgaccg
      361 aaaaaaacct aaaaatagag aaagcatccg ccgccgtcgt tgacgccacc attattcaga
      421 c
//
LOCUS       DAXHSF010000292          421 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.292, whole genome shotgun sequence.
ACCESSION   DAXHSF010000292 DAXHSF010000000
VERSION     DAXHSF010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.292"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(<1..350)
                     /locus_tag="ACFM8S_002074"
     CDS             complement(<1..350)
                     /locus_tag="ACFM8S_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFC6315073.1"
                     /translation="MTIQQPELKNPTIVVVTDRNDLDGQLFQTFSSGKDLIKQTPQQV
                     EDREQLRQLLAQNEVGGVFFTTIQKFALNEEESRFPVLNERSNIIVISDEAHRSQYGF
                     TQKLYNGKFQAGYAR"
BASE COUNT          106 a           88 c           94 g          133 t
ORIGIN      
        1 cgagcataac ctgcttggaa tttgccgtta tatagttttt gtgtaaaacc gtattggctg
       61 cggtgtgcct catcgctaat cacaataata ttgctgcgtt catttaagac agggaagcgg
      121 ctttcttcct cgtttaaggc gaatttttga atggtagtaa aaaacacgcc tcccacttca
      181 ttttgtgcga gcagttggcg tagttgttct cggtcttcta cttgttgcgg tgtttgtttg
      241 attaaatctt tgccagaaga aaaggtttgg aacaactgac cgtccaaatc gttgcggtcg
      301 gtaactacca caatcgtcgg attttttaat tcaggctgtt gaatcgtcat tcccgcgcac
      361 gcgggaatcc agaccttaga acaacagcaa tattcaaaga ttatctgaaa gtccgagatt
      421 c
//
LOCUS       DAXHSF010000293          417 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.293, whole genome shotgun sequence.
ACCESSION   DAXHSF010000293 DAXHSF010000000
VERSION     DAXHSF010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.293"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            1..417
                     /locus_tag="ACFM8S_002075"
                     /pseudo
     CDS             1..417
                     /locus_tag="ACFM8S_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          136 a          121 c           96 g           64 t
ORIGIN      
        1 accgaaaaaa acctaaaagt agagaaagca tccgcctccg tcgttgacgc caccattatt
       61 cagaccgccg gcagcaaaca gcgtcaggcc atagaagttg acgaataagg acaaatcaac
      121 ggccaaacca caccgagtaa ggacagcgat gcccgttgga tcaagaaaaa cggcctctac
      181 aaactcggtt acaaacaaca tacccgcacc gatgcggaag gctatatcga gaaactgcac
      241 attacccccg ccaatgccca tgagtgcaac cacctgttgc ctttgctgga aggtatagcc
      301 gaaggcacga ccgtctatgc cgacaaaggc tacgacagta aggaaaaccg gcagcatctg
      361 aaagaacatc agttactgga cggcattatg cgcaaagcct gccgcaaccg tccgctg
//
LOCUS       DAXHSF010000294          405 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.294, whole genome shotgun sequence.
ACCESSION   DAXHSF010000294 DAXHSF010000000
VERSION     DAXHSF010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.294"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            112..327
                     /gene="rpmE"
                     /locus_tag="ACFM8S_002076"
     CDS             112..327
                     /gene="rpmE"
                     /locus_tag="ACFM8S_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFC6315074.1"
                     /translation="MKQGIHPNYHEVNVTCSCGNKFATKSAMEKENFNIEVCSLCHPF
                     YTGTQKIVDTTGRVDKFNNKFGNLFKR"
BASE COUNT          126 a          106 c           74 g           99 t
ORIGIN      
        1 atacatttta tcgaatatcc tcttgacgaa acaacagaaa atagcgagaa tgccgagctt
       61 gtctgaagca taggtacatc ccctatgccc gcttttttga aaggaaatca aatgaaacaa
      121 ggtattcacc cgaactacca cgaagtcaac gttacctgct cttgcggcaa caaattcgca
      181 accaaatccg cgatggaaaa agaaaacttc aatatcgagg tttgctccct gtgccacccg
      241 ttctataccg gcacccaaaa aatcgtcgat accaccggcc gcgtggacaa attcaacaac
      301 aaattcggca acctgttcaa acgctgattg ccgctttgca agaattaagc cctgccatca
      361 tttcgatggc agggcttttt aatatttggc acactgactt cttat
//
LOCUS       DAXHSF010000295          401 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.295, whole genome shotgun sequence.
ACCESSION   DAXHSF010000295 DAXHSF010000000
VERSION     DAXHSF010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.295"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..>401
                     /locus_tag="ACFM8S_002077"
     CDS             <1..>401
                     /locus_tag="ACFM8S_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6315075.1"
                     /translation="TNDTYDHLAKNIYQNLLFQTRLQPYLNQISFKMENDTFTLDFSG
                     LVQAFNHVKETNPQKAFVDLAEMLAYGELRSWYEGRRLMADYVEEAKKAGKFEDYQKV
                     LGQETVALLAKTSGTQADDILQNVGFGHNKNV"
BASE COUNT          134 a           74 c           85 g          108 t
ORIGIN      
        1 accaacgata catacgacca tctcgccaaa aacatctacc aaaacctgtt attccaaacc
       61 cgtttgcagc catatttgaa tcaaatcagt ttcaaaatgg aaaatgatac gttcactttg
      121 gattttagtg gtcttgttca agcatttaac catgtcaaag aaactaatcc gcaaaaagct
      181 tttgtggatt tggccgagat gcttgcatat ggcgaacttc gttcttggta tgaaggccga
      241 agactaatgg ccgattatgt ggaggaggca aaaaaagcag gtaaatttga agattaccag
      301 aaagtgttgg gtcaggagac cgttgcatta ttagctaaaa catcgggtac gcaagcagat
      361 gatatcctgc aaaatgtagg ctttggtcat aataaaaatg t
//
LOCUS       DAXHSF010000296          393 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.296, whole genome shotgun sequence.
ACCESSION   DAXHSF010000296 DAXHSF010000000
VERSION     DAXHSF010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.296"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
BASE COUNT           79 a           96 c          125 g           93 t
ORIGIN      
        1 cacgtcattc ccacggaagt gggaatctag aatctcgggg gtttcagtca tttccgatag
       61 attcccgccg cgtcggaggt ctggattccc gcctgtgcgg gaatgacggg tttcgagatt
      121 acggtgttgt cggaacgaaa ctgaaccgtc attcccacgg aagtgggaat ctagaatctc
      181 ggggtttcag tcatttccga tagattcccg ccgcgtcggg ggtctggatt cccgcctgtg
      241 cgggaatgac gggtttcaag attacggtgt tgtcgggaat gacggttcgg gtatttccac
      301 gcccgccccg cgcctgtaaa cggcgagtgc atcaaaaatg ccgtctgaag gttcagacgg
      361 catcggtgtc ggggaatcag aagtggtagc gca
//
LOCUS       DAXHSF010000297          381 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.297, whole genome shotgun sequence.
ACCESSION   DAXHSF010000297 DAXHSF010000000
VERSION     DAXHSF010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.297"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(96..290)
                     /gene="thiS"
                     /locus_tag="ACFM8S_002078"
     CDS             complement(96..290)
                     /gene="thiS"
                     /locus_tag="ACFM8S_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFC6315076.1"
                     /translation="MNIILNGEPAELHGTSVADLIAQTAPQKPFAVAVNTVFVPKGAY
                     AETVLNENDKIDIVRPVVGG"
BASE COUNT           80 a           90 c          105 g          106 t
ORIGIN      
        1 gtactgtctg cggcttcgtc gccttgtcct gatttaaagt taatccacca taacgttgtt
       61 ttggggacag gggtcgtctg aaaaggcaaa accgcctagc cgccgaccac cggccgcacg
      121 atatcgattt tgtcgttttc gtttaaaacc gtttccgcat acgcgccttt ggggacgaaa
      181 acggtgttga ccgccacggc aaagggcttt tgcggcgcgg tttgggcgat gaggtcggca
      241 acgcttgtgc cgtgaagttc ggcgggttcg ccgtttaaga tgatgttcat ggtttctcga
      301 tgtttctgta tttatagtgg attaacaaaa accagtacgg cgttgcctcg ccttagctca
      361 aagaaaacga ttctctaagg t
//
LOCUS       DAXHSF010000298          374 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.298, whole genome shotgun sequence.
ACCESSION   DAXHSF010000298 DAXHSF010000000
VERSION     DAXHSF010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.298"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
BASE COUNT          105 a           84 c           79 g          106 t
ORIGIN      
        1 tttttttgaa tttcgggcaa cgcttgaatc ttcattccgc gcaggcggaa attatcggtg
       61 cggtacggca acttttttcg atatgaaaag accgtcattc ctgtaaaaac aaaaaatcaa
      121 aaacagaaaa ttgaaattcg tcattcccgc gcaggcggga atctaggacg taaaatctat
      181 agtggattaa caaaaaccag tacagcgttg ccttgactta gctcgaagag aacgattctc
      241 taaggtgctg aagcaccgag tgagtcggtt ccgcactatc cgtactgtct gcggcttcgt
      301 cgctttctcc tgatttttgt taatccacta taaagaaacc gtttttctcg ataagtttcc
      361 gtgccgacag acct
//
LOCUS       DAXHSF010000299          364 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.299, whole genome shotgun sequence.
ACCESSION   DAXHSF010000299 DAXHSF010000000
VERSION     DAXHSF010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.299"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(<1..283)
                     /locus_tag="ACFM8S_002079"
     CDS             complement(<1..283)
                     /locus_tag="ACFM8S_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFC6315077.1"
                     /translation="MLKQTTLLAACASFAALLGGCATQQPAPVIAGNSGMQDAPSSAV
                     YNNPYGATPYSPAPAGDAPYVPPVQSAPVYTPPAYVPPSAPAVSGTYVPS"
BASE COUNT           97 a           92 c          108 g           67 t
ORIGIN      
        1 aagaaggaac gtatgtaccc gaaacggcag gtgcagacgg cggaacataa gcaggaggcg
       61 tataaaccgg cgcgctttgc accggcggca cataaggcgc atcgccggca ggagccgggc
      121 tgtacggcgt tgctccatag gggttgttgt aaactgccga agacggcgcg tcctgcatac
      181 ctgaattgcc tgcaatgacg ggggcaggct gctgggtagc acaaccgccc aacagtgcgg
      241 caaaggaggc gcaagccgcc aaaagtgtcg tttgtttcaa cataagataa ccttcatgtt
      301 ccgatatata gcctgaatgc ggtatatcat aataaaaatg cgcgttcttc tcaagcgcaa
      361 agcc
//
LOCUS       DAXHSF010000300          354 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.300, whole genome shotgun sequence.
ACCESSION   DAXHSF010000300 DAXHSF010000000
VERSION     DAXHSF010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.300"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..>354
                     /locus_tag="ACFM8S_002080"
     CDS             <1..>354
                     /locus_tag="ACFM8S_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6315078.1"
                     /translation="FPLLKLDQVIDWQPIEQYLNRQKNRYLRDHRGRPAYPLLSMFKA
                     VLLGQWHSLSDPELEHSLITRIDFNLFCRFDELSIPDYSTLCRYRNRLAQDNTLSELL
                     ELINRQLTEKGLKIEK"
BASE COUNT           93 a          110 c           73 g           78 t
ORIGIN      
        1 ttcccgctat tgaagttgga tcaggtaatt gattggcagc cgatcgagca gtacctgaac
       61 cgtcaaaaaa accgttacct ccgagaccac cgcggccgtc ctgcctatcc cctgctgtcc
      121 atgttcaaag ccgtcctgct cggacaatgg cacagcctct ccgatcccga actcgaacac
      181 agcctcatta cccgcatcga tttcaacctg ttttgccgtt ttgacgaact gagcatcccc
      241 gattacagca ccttatgccg ctaccgcaac cggctggcgc aagacaatac cctgtctgaa
      301 ctgttggaac tgattaaccg ccaactgacc gaaaaaggtt taaaaataga gaaa
//
LOCUS       DAXHSF010000301          341 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.301, whole genome shotgun sequence.
ACCESSION   DAXHSF010000301 DAXHSF010000000
VERSION     DAXHSF010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.301"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            141..341
                     /locus_tag="ACFM8S_002081"
                     /pseudo
     CDS             141..341
                     /locus_tag="ACFM8S_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
BASE COUNT          112 a          101 c           71 g           57 t
ORIGIN      
        1 acagcggcaa cgaaaaaatc gacaccaagc acctgccgtc aacagcaccg acaagaaaat
       61 cgacaccaaa ctagagaaac ctttttaccc gataagtttc cgtaccgaca aggctggatt
      121 cccgcctgcg cgggaatgac gaagctatcc ttttggccga aggtcaaaaa tcagccgtca
      181 ccgagtatta cctgaatcac ggcatatggc ccgccaacaa caattctgcc ggcgtggcat
      241 ccaccgccac cgacatcaaa ggcaaatatg ttcaaagcgt tgaagtcaaa aacggcgtcg
      301 ttaccgccac aatggcttca agcaacgtaa acaacgaaat c
//
LOCUS       DAXHSF010000302          331 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.302, whole genome shotgun sequence.
ACCESSION   DAXHSF010000302 DAXHSF010000000
VERSION     DAXHSF010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.302"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..>331
                     /locus_tag="ACFM8S_002082"
     CDS             <1..>331
                     /locus_tag="ACFM8S_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFC6315079.1"
                     /translation="RLDSRLRGNDEAILLAEGQKSAVTEYYLNHGEWPANNSSAGVAT
                     SASDIKGKYVQSVEVKNGVITATMLSSGVNNEIKGKKLSLWATRQDGSVKWFCGQPVT
                     RDAATDDD"
BASE COUNT           91 a           97 c           85 g           58 t
ORIGIN      
        1 aggctggatt cccgcctgcg cgggaatgac gaagccattc ttttggctga aggtcaaaaa
       61 tcagccgtca ccgagtatta cctgaatcac ggcgaatggc ccgccaacaa cagttctgcc
      121 ggcgtggcaa cctccgcctc cgacatcaaa ggcaaatatg ttcaaagcgt tgaagtcaaa
      181 aacggcgtca ttaccgccac aatgctttca agcggcgtaa acaacgaaat caaaggcaaa
      241 aaactctccc tgtgggccac gcgtcaagac ggttcggtaa aatggttctg cggacagccg
      301 gttacgcgcg acgccgccac cgacgacgac g
//
LOCUS       DAXHSF010000303          331 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.303, whole genome shotgun sequence.
ACCESSION   DAXHSF010000303 DAXHSF010000000
VERSION     DAXHSF010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.303"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
BASE COUNT           96 a           76 c           94 g           65 t
ORIGIN      
        1 gctaagtgcg cccgctgccg cctaaaagga gaccggatgc ctgattatcg ggtatccggg
       61 gagggttaag ggggtatttg ggtaaaatca ggaggtattt gaggcgaaaa cagccgaaaa
      121 cctgtgtttg ggtttcggct gtcgggaggg aaaggaattt tgcaaagatc tcatcctgtt
      181 attttcacaa aaacagaaaa ccaaaaacag caacctgaaa ttcgtcattc ccgcgcaggc
      241 gggaatccag acccccaacg cggcaggaat ctatcggaaa taaccgaaac cggacgaacc
      301 tagattcccg ctttcgcggg aatgacggca g
//
LOCUS       DAXHSF010000304          330 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.304, whole genome shotgun sequence.
ACCESSION   DAXHSF010000304 DAXHSF010000000
VERSION     DAXHSF010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.304"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(126..>330)
                     /locus_tag="ACFM8S_002083"
     CDS             complement(126..>330)
                     /locus_tag="ACFM8S_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6315080.1"
                     /translation="IGGQSTAAIADLAGEMRRLAKKSKMLLKGLWPFLSSTISAFKIN
                     QSLFKHYCKTVQLLNCKHRKKNY"
BASE COUNT          102 a           58 c           65 g          105 t
ORIGIN      
        1 cgaaaaaacg cgacgtaacc agcatcaaca tatataagaa cagcacaaat agcatcaata
       61 catcaggcaa cgaaaatgca gaataatgca cttaatggtg tttggatatc tgttgttttg
      121 tgctgttagt aattcttctt tctgtgttta cagtttagca gttgtacagt tttacagtaa
      181 tgtttaaaca atgactgatt tattttaaat gcagatattg tagaggataa aaatggccaa
      241 agtcctttca gtaacatttt tgatttttta gcgagccttc tcatttcccc ggcgagatcg
      301 gcaatggcag cggtactttg gccgccgata
//
LOCUS       DAXHSF010000305          327 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.305, whole genome shotgun sequence.
ACCESSION   DAXHSF010000305 DAXHSF010000000
VERSION     DAXHSF010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.305"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
BASE COUNT           89 a           96 c           71 g           71 t
ORIGIN      
        1 agacctgtcg gcacagaaac ttatcgggca aaacggtttc ttgagatttt acgtcctaga
       61 ttcccgcctg cgcgggaatg acgcgtttca tttgccgccc cctcccgaaa aacgcaaaaa
      121 atgccgtccg aaaacctttc ggacggcatt ttcgcgtgaa ccgtcattcc cacgaaaaca
      181 gaaaaccaaa atcagaaacc taaaattcgt cattcccacg aaagtgggaa tccaggactc
      241 aggacctgag aaaccttttt acccgataag ttcccgtacc gaccggtccg gattcccgcc
      301 tgcgcgggaa tgacgcgttt catttgc
//
LOCUS       DAXHSF010000306          318 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.306, whole genome shotgun sequence.
ACCESSION   DAXHSF010000306 DAXHSF010000000
VERSION     DAXHSF010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.306"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(<1..266)
                     /locus_tag="ACFM8S_002084"
     CDS             complement(<1..266)
                     /locus_tag="ACFM8S_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX iron-regulated FrpC family protein"
                     /protein_id="HFC6315081.1"
                     /translation="MTSCEPVNEKTDQKAVSAQQAKEQTSFNNPEPMTGFEHTVTFDF
                     QGTKMVIPYGYLARYTQDNATKWLSDTPGQDAYSINLIEISVYY"
BASE COUNT           90 a           61 c           67 g          100 t
ORIGIN      
        1 ttgtaataga cgctaatctc tatcaaatta atggagtaag catcctgccc gggcgtgtcg
       61 gaaagccatt ttgtggcatt gtcttgcgta taccgtgcaa gatagccata ggggataacc
      121 attttggtgc cctgaaaatc aaatgtaacc gtatgttcaa atcctgtcat tggctcggga
      181 ttgttgaaac tggtttgttc tttagcctgt tgcgcactta ctgctttttg atctgtcttt
      241 tcattcacag gttcacatga ggtcatagta aaaacactcc caataaacaa aataaaaagt
      301 tgataaatgg tagtagca
//
LOCUS       DAXHSF010000307          308 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.307, whole genome shotgun sequence.
ACCESSION   DAXHSF010000307 DAXHSF010000000
VERSION     DAXHSF010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.307"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..>308
                     /locus_tag="ACFM8S_002085"
     CDS             <1..>308
                     /locus_tag="ACFM8S_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC6315082.1"
                     /translation="IPDYSTLCRYRNWLAQDDTLSELLKLINCQLTEKGLKIEKASAA
                     VVDATIIQTAGSKQRQAIEVDEEGQISGQTTPSKDSDARWIKKNGLYKLGYKQHTRTD
                     "
BASE COUNT          102 a           91 c           66 g           49 t
ORIGIN      
        1 catccccgat tacagtacct tatgccgcta ccgcaactgg ctggcgcaag acgacaccct
       61 gtccgaatta ctcaaactga ttaactgcca actgaccgaa aaaggcctaa aaatagagaa
      121 agcatccgcc gccgtcgttg atgccaccat tatccagacc gccggcagca aacagcgtca
      181 ggccatagaa gttgacgaag aaggacaaat cagcggtcaa accacaccga gtaaggacag
      241 cgatgcccgt tggataaaga aaaacggcct ctacaaactc ggttacaaac aacatacccg
      301 taccgatg
//
LOCUS       DAXHSF010000308          306 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.308, whole genome shotgun sequence.
ACCESSION   DAXHSF010000308 DAXHSF010000000
VERSION     DAXHSF010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.308"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
BASE COUNT           91 a           78 c           59 g           78 t
ORIGIN      
        1 catttccgag aaattgcctt agcattgaat gtctagattc ccgccttcgc gggaatgacg
       61 aagtggaagt tacccgaaac ttaaaacaag cgaaaccgaa cgaactagat tcccggactt
      121 tcagataatc tttgaatatt gctgttgttc taaggtctag attcccgcct tcgcgggaat
      181 gacgaatcca tccatacgga aacctgcatc acgtcattcc tacgaaccta cattccgtca
      241 ttcccacgaa agtgggaatc tagaacgtaa aatctcaaga aaccgtttta cccgataagt
      301 ttccgc
//
LOCUS       DAXHSF010000309          305 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.309, whole genome shotgun sequence.
ACCESSION   DAXHSF010000309 DAXHSF010000000
VERSION     DAXHSF010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.309"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            63..305
                     /locus_tag="ACFM8S_002086"
                     /pseudo
     CDS             63..305
                     /locus_tag="ACFM8S_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238520.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
BASE COUNT           82 a           85 c           76 g           62 t
ORIGIN      
        1 cggtttcttg agattttgcg ttctagattc ccactttcgt gggaatgacg ggatttaatg
       61 atgccgccgg cgtggcatcc tccccctccg acatcaaagg caaatatgtt gagaaagttg
      121 aagtcgcaaa cggcgtcatt accgcccaaa tggcttcaag caacgtaaac aacgaaatca
      181 aaggcaaaaa actctctctg tgggccaagc gtcaagacgg ttcggtaaaa tggttctgcg
      241 gacagccggt tacgcgcgac gcccccaacg ccagcgccga cgccgttaac aaagtcaccg
      301 gcaac
//
LOCUS       DAXHSF010000310          298 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.310, whole genome shotgun sequence.
ACCESSION   DAXHSF010000310 DAXHSF010000000
VERSION     DAXHSF010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.310"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..>298
                     /locus_tag="ACFM8S_002087"
     CDS             <1..>298
                     /locus_tag="ACFM8S_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6315083.1"
                     /translation="QNKLIELFVAGVTARTAAELVGVNKSTAAYYFHRLRLLIYQNSP
                     HLEMFDGEVEADESYFAERQNHINGIGNFWNRAKRHLRKFDGIPKAHFELYLKG"
BASE COUNT           99 a           50 c           65 g           84 t
ORIGIN      
        1 aacaaaataa actcattgag ttatttgtcg caggtgtaac tgcaagaaca gcagcagagt
       61 tagtaggcgt taataaaagt accgcagcct attattttca tcgtttacga ttacttattt
      121 atcaaaacag tccgcatttg gaaatgtttg acggcgaagt agaagcagat gaaagttatt
      181 ttgctgaacg acaaaaccat atcaatggaa ttgggaactt ttggaaccgg gcaaaacgtc
      241 atttacgcaa gtttgacggc attcccaaag cgcattttga gctgtattta aaggggta
//
LOCUS       DAXHSF010000311          296 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.311, whole genome shotgun sequence.
ACCESSION   DAXHSF010000311 DAXHSF010000000
VERSION     DAXHSF010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.311"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..>296
                     /locus_tag="ACFM8S_002088"
     CDS             <1..>296
                     /locus_tag="ACFM8S_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248696.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
                     /protein_id="HFC6315084.1"
                     /translation="TRSIRQRISDNYSNLGSNFSDRADEANRKMFEHNAKLDRWGNSM
                     EFINGVAAGALNPFISAGEAVDQWMQENPNAAETVEALVNVLPFAKVKNLTKAA"
BASE COUNT           82 a           97 c           74 g           43 t
ORIGIN      
        1 acccgcagca tccggcaacg catatccgac aattacagca acctcggcag caatttctcc
       61 gaccgcgccg atgaagccaa cagaaaaatg ttcgagcaca atgccaagct cgaccgctgg
      121 ggcaacagca tggagtttat caacggcgtc gccgccggcg cgctcaaccc ctttatcagc
      181 gcgggcgaag ccgttgacca gtggatgcag gaaaacccca atgccgccga aaccgtcgaa
      241 gccctggtca acgtcctgcc gtttgccaaa gtcaaaaacc tgacaaaggc ggcaaa
//
LOCUS       DAXHSF010000312          287 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.312, whole genome shotgun sequence.
ACCESSION   DAXHSF010000312 DAXHSF010000000
VERSION     DAXHSF010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.312"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(<1..>287)
                     /locus_tag="ACFM8S_002089"
     CDS             complement(<1..>287)
                     /locus_tag="ACFM8S_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFC6315085.1"
                     /translation="GDFRTGSVGVTLLDGSRHAPNPVKEIEVARVLQNIGLQSGSTTE
                     AAVRFMLYCMRQQVFWDGNKRTATLFANGLMMAGGCGILEISEMQMPQFNE"
BASE COUNT           68 a           85 c           66 g           68 t
ORIGIN      
        1 ttttcattga attgcggcat ctgcatttcg gagatttcca agatgccgca gccccccgcc
       61 atcatcagac cgttggcaaa taaggttgcc gttcgtttgt tgccgtccca aaaaacctgc
      121 tgccgcatac aataaagcat aaaacggacg gctgcctccg tcgtcgaacc gctttgcagt
      181 ccgatatttt gtaacacgcg ggccacttca atttccttca ctggattcgg ggcatgacgg
      241 gaaccgtcca atagcgttac gccgaccgaa ccggtacgga aatcacc
//
LOCUS       DAXHSF010000313          284 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.313, whole genome shotgun sequence.
ACCESSION   DAXHSF010000313 DAXHSF010000000
VERSION     DAXHSF010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.313"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            186..257
                     /locus_tag="ACFM8S_002090"
                     /pseudo
     CDS             186..257
                     /locus_tag="ACFM8S_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696591.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
BASE COUNT           62 a           66 c           90 g           66 t
ORIGIN      
        1 gtttgcgcct aaaaggcggc ccggatgcct gattatcggg tatccgggca ggattaaggg
       61 ggtattgggg taaaattagg aggtatttgg ggcggaaata gctgaaaacc tgtgtttggg
      121 tttcggctgt cgggagggaa aggaattttg caaaggtctc gggctatgcc ggctgctgcc
      181 gcccattgca ccttcgccga atcctgccgc ccaccgccga agccctcatg cgttcgcggt
      241 acagcgcgta tgtgctacta tagtggatta acaaaaacca gtac
//
LOCUS       DAXHSF010000314          282 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.314, whole genome shotgun sequence.
ACCESSION   DAXHSF010000314 DAXHSF010000000
VERSION     DAXHSF010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.314"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(1..111)
                     /locus_tag="ACFM8S_002091"
                     /pseudo
     CDS             complement(1..111)
                     /locus_tag="ACFM8S_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(148..>282)
                     /locus_tag="ACFM8S_002092"
     CDS             complement(148..>282)
                     /locus_tag="ACFM8S_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6315086.1"
                     /translation="NPVKHGYVGQISDWGFSTFHRYVKQGIYPHNWGGGNADFSIGYD
                     "
BASE COUNT           70 a           72 c           61 g           79 t
ORIGIN      
        1 cacataacgg gtcttcgata aatatcggtt acgcttggtt tgattttccg tcagcggacg
       61 gttgcggcag gctttgcgca taatgccgtc ctgcagccga tgctctttca gttttccgta
      121 ggtcggattc tcgaatccga cattacttca atcgtatcca atagaaaagt ccgcattgcc
      181 gccaccccaa ttatgcggat aaataccctg tttgacataa cggtgaaacg tagaaaaccc
      241 ccaatcggaa atttgtccta catagccatg tttgaccgga tt
//
LOCUS       DAXHSF010000315          275 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.315, whole genome shotgun sequence.
ACCESSION   DAXHSF010000315 DAXHSF010000000
VERSION     DAXHSF010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.315"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
BASE COUNT           89 a           86 c           40 g           60 t
ORIGIN      
        1 tatcccgtca ttcctacgaa cctacatccc gtcattccct caaaaacaga aaaccaaaat
       61 tagaaaccta aaatcccgtc attcccgcgc aggcgggaat ccaaacttgt ccgcacggaa
      121 acttatcgga taaaacggtt tcttagattc cacgttctag attcccgcct gcgcgggaat
      181 gacgaatcca tccgcacgga aacctatatc ccgtcattct tacgaaccta catcccgtca
      241 ttccctcaaa aacagaaaac caaaattaga aacct
//
LOCUS       DAXHSF010000316          268 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.316, whole genome shotgun sequence.
ACCESSION   DAXHSF010000316 DAXHSF010000000
VERSION     DAXHSF010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.316"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
BASE COUNT           78 a           66 c           67 g           57 t
ORIGIN      
        1 aggcttacat caaagccgat aacgctttca caaagcctgt gtgcactgaa aactgaaagg
       61 tcgtctgaaa acggagcttt cgaaggaagc agagtttctg cgaagctaaa accgaagctg
      121 caaaaaatcg aaaagcagcc tgcacgttga aaatgccgcc caagcaaacc tgcggtttgc
      181 cgccctctcc ctagccctct cccacaggga gaggggattg gggtgcaggc tgtctttaag
      241 gttcaggcaa atttttaact tcgttgaa
//
LOCUS       DAXHSF010000317          263 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.317, whole genome shotgun sequence.
ACCESSION   DAXHSF010000317 DAXHSF010000000
VERSION     DAXHSF010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.317"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
BASE COUNT           78 a           49 c           75 g           61 t
ORIGIN      
        1 ctaaaggcga tgtgtttgaa ccttttgaaa gccgccaaca ggctaagtgc gcccgctgcc
       61 gcctaaaagg cagcacggat gcctgattat caggtgtccg gggaggatta agggggtatt
      121 tgggtaaaat taggaggtat ttggggcgaa aacagctgaa aacctgtgtt tgggtttcgg
      181 ctgtcgggag ggaaaggaat tttgcaaagg tctcatcctg ttattttcac aaaaacagaa
      241 aaccaaaaac agcaacctga aat
//
LOCUS       DAXHSF010000318          257 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.318, whole genome shotgun sequence.
ACCESSION   DAXHSF010000318 DAXHSF010000000
VERSION     DAXHSF010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.318"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
BASE COUNT           74 a           68 c           55 g           60 t
ORIGIN      
        1 gttttcccga taaatgcaca tcatccaaag tcccgttatt cccacaaaaa cagaaaatca
       61 aaaacagcaa cctgaaatcc cgtccttccc gcgcaggcgg taatctgaac acgtccgtag
      121 tgaaacctat atcccgtcat tcgcacgaaa gtgggaatcc aggatgcagg gaaaaccgtt
      181 ttatccgata agtttccgca ccgaaaggtc tagattcccg ctttcgcggg aatgacggcg
      241 gagggttttt agttttc
//
LOCUS       DAXHSF010000319          253 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.319, whole genome shotgun sequence.
ACCESSION   DAXHSF010000319 DAXHSF010000000
VERSION     DAXHSF010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.319"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(57..>253)
                     /locus_tag="ACFM8S_002093"
     CDS             complement(57..>253)
                     /locus_tag="ACFM8S_002093"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6315087.1"
                     /translation="PRGLDSRVRGNDGIFFAFDIKGRLNFVQQVQSVAHKRAQDRGKA
                     GVGRAVATVFFTPSGKNIVD"
BASE COUNT           65 a           72 c           49 g           67 t
ORIGIN      
        1 accttagaga atcgttctct ttgagctaag gcgaggcaac gccgtactgg tttttgttaa
       61 tccactatat ttttgcccga cggggtgaaa aatacagttg ctacagcccg acctacgccc
      121 gctttgcctc tatcctgcgc ccttttatgt gcaacacttt gcacttgctg aacaaaattc
      181 aaacgaccct ttatatcaaa tgcaaaaaat atgccgtcat tcccgcgaac gcgggaatcc
      241 agacccctcg gca
//
LOCUS       DAXHSF010000320          250 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.320, whole genome shotgun sequence.
ACCESSION   DAXHSF010000320 DAXHSF010000000
VERSION     DAXHSF010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.320"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..>250
                     /locus_tag="ACFM8S_002094"
     CDS             <1..>250
                     /locus_tag="ACFM8S_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFC6315088.1"
                     /translation="PKITRVDIAKDFFNGEYSPNQAREDRNKGMFTCHHVKPKGECLG
                     SDWEEDDEAKMTKGKTYGIGSRESSKYVRVYEKGKQLG"
BASE COUNT           85 a           58 c           61 g           46 t
ORIGIN      
        1 gcccaaaaat cacacgcgtt gacatcgcaa aagacttttt caacggcgaa tacagcccga
       61 accaagcccg tgaagaccga aataaaggta tgtttacctg tcatcacgtc aaaccaaaag
      121 gcgaatgttt ggggtcagat tgggaagaag acgatgaagc caaaatgacc aaaggcaaga
      181 cctatggtat cggctcccgt gaatcgtcca aatatgtccg cgtctatgaa aaaggcaagc
      241 agttgggcga
//
LOCUS       DAXHSF010000321          241 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.321, whole genome shotgun sequence.
ACCESSION   DAXHSF010000321 DAXHSF010000000
VERSION     DAXHSF010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.321"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
BASE COUNT           68 a           62 c           64 g           47 t
ORIGIN      
        1 ccgcgaacgc gggaatctag aacgtagaat ctgagaaacc gttttactcg ataaatttcc
       61 gtgccgacag gtctggattc ccgccttcgc gggaatgacg gcatcagttt gcaggattcg
      121 gcggaaacgg taaaaacggc agaatcgatg ggatgcggca ggctgaagcc caccaaaaca
      181 caaaaattcc gatgccgtct gaaatttcgt cattcccgtg aaaacgggaa tccagccccg
      241 t
//
LOCUS       DAXHSF010000322          240 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.322, whole genome shotgun sequence.
ACCESSION   DAXHSF010000322 DAXHSF010000000
VERSION     DAXHSF010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.322"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
BASE COUNT           83 a           51 c           64 g           42 t
ORIGIN      
        1 actcaaagct gcaagaattt atcagaaaca actggaactt aaaaaactag attcccgcct
       61 gcgcgggaat gacgaagggt taaagaaatg acgagaaacg gcggtaattt atcgggaata
      121 accgaaaacg aacggaaatc aggacaaggc ggcgaagccg cagacagtac ggatagtgcg
      181 gaaccgactc actcggtgct tcagcacctt agagaatcgt tctctttgag ctaaggcgag
//
LOCUS       DAXHSF010000323          226 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.323, whole genome shotgun sequence.
ACCESSION   DAXHSF010000323 DAXHSF010000000
VERSION     DAXHSF010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..226
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.323"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
BASE COUNT           58 a           51 c           50 g           67 t
ORIGIN      
        1 gaaaccattt tatagtggat taacaaaaac cagtacggcg ttgcctcgcc ttagctcaaa
       61 gagaatgatt ctctaaggtg ctgaagcacg agtgaatcgg ttccgtatta tttgtactgt
      121 ctgcggcttc gtcgccttgt cctgattttt gttaatccac tatatccgat aagtttccgc
      181 accgacaaaa ctagattccc actttcgtgg gaatgacggg atgcag
//
LOCUS       DAXHSF010000324          219 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.324, whole genome shotgun sequence.
ACCESSION   DAXHSF010000324 DAXHSF010000000
VERSION     DAXHSF010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..219
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.324"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
BASE COUNT           65 a           46 c           51 g           57 t
ORIGIN      
        1 tttttggttt tctgttttcg cgggaataac ggaattttaa gttttaggaa tttgtcggaa
       61 aaacagaaat ccccccgccg tcattcccgc gcaggcggga atctagaaat ttaacgttgc
      121 ggtgatttat cggaaatgac tgaaactcaa cgaaccggat tcccactttc gcgggaatga
      181 caaagtggaa gttgcccgaa acttaaaaca agcgaaacc
//
LOCUS       DAXHSF010000325          217 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.325, whole genome shotgun sequence.
ACCESSION   DAXHSF010000325 DAXHSF010000000
VERSION     DAXHSF010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.325"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            complement(30..>217)
                     /locus_tag="ACFM8S_002095"
     CDS             complement(30..>217)
                     /locus_tag="ACFM8S_002095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6315089.1"
                     /translation="NYKVSLPPVKISLDIPNILCYTFQFVRHFCSSEEPNYTPTGKNG
                     QCFQWDFFGEIRHVAVG"
BASE COUNT           68 a           44 c           53 g           52 t
ORIGIN      
        1 ggcgcagcat ttagcagaaa taaaaaacct tatccgacag cgacatgacg aatttcccca
       61 aaaaaatccc actgaaagca ttgaccgttt ttccctgtgg gcgtatagtt cggttcttcg
      121 ctgctgcaga agtggcggac gaactgaaaa gtatagcaca gaatgttggg gatatcgaga
      181 gatatcttga caggcggaag agatacttta taattcg
//
LOCUS       DAXHSF010000326          216 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M05 240370
            SAMEA1706904-rid23332093.denovo.326, whole genome shotgun sequence.
ACCESSION   DAXHSF010000326 DAXHSF010000000
VERSION     DAXHSF010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706904
            Sequence Read Archive: ERR484094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378082.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:40:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,041
            Genes (coding)                    :: 1,936
            CDSs (with protein)               :: 1,936
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 40 of 105
            Pseudo Genes (incomplete)         :: 74 of 105
            Pseudo Genes (internal stop)      :: 10 of 105
            Pseudo Genes (multiple problems)  :: 18 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..216
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706904-rid23332093.denovo.326"
                     /strain="M05 240370"
                     /db_xref="taxon:487"
     gene            <1..>216
                     /locus_tag="ACFM8S_002096"
     CDS             <1..>216
                     /locus_tag="ACFM8S_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC6315090.1"
                     /translation="RVAYGHVKHQVHSVETKTTTVTSKPNGKNLKGGPVSSREPSKPP
                     YHESHSTSSLGLGVIAGVGFDITPKLT"
BASE COUNT           63 a           64 c           48 g           41 t
ORIGIN      
        1 gcgcgtcgcc tacggacacg ttaaacatca ggttcattcg gtggaaacaa aaaccacgac
       61 tgttacctct aaaccaaacg gaaaaaatct aaagggagga cctgtctcat caagagagcc
      121 cagcaaacct ccctatcacg aaagccacag caccagcagc ttgggtcttg gtgtcatcgc
      181 cggtgtcggt ttcgacatca cgcccaagct gacctt
//
